title: [Pathological Diagnoses and Whole-genome Sequence Analyses of the Jaagsiekte Sheep Retrovirus in Xinjiang, China].
abstract: To carry out pathologic diagnoses and whole-genome sequence analyses of the Jaagsiekte sheep retrovirus (JSRV) in Xinjiang, China, we first observed sheep suspected to have the JSRV. Then, the extracted virus suspension was observed by transmission electron microscopy (TEM). Total RNAs from lungs of JSRV-infected sheep were extracted and reverse-transcribed using a cDNA synthesis kit. Six pairs of primers were designed according to the exogenous reference virus strain (AF105220). Reverse transcription-polymerase chain reaction was carried out from JSRV-infected tissue, and the whole genome of the JSRV sequenced. Our results showed: flow of nasal fluid ("wheelbarrow test"); different sizes of adenoma lesions in the lungs; papillary hyperplasia of alveolar epithelial cells; alveolar cavity filled with macrophages; dissolute nuclei in central lesions. TEM revealed JSRV particles with a diameter of 88 nm to 125. 4 nm. The full-length of the viral genome sequence was 7456 bp. BLAST analyses showed nucleotide homology of 96% and 95% compared with that of the representative strain from the USA (AF105220) and UK (AF357971). Nucleotide homology was 89.8% and 89.9% compared with the endogenous Jaagsiekte sheep retrovirus, Inner Mongolia strain (DQ838493) and USA strain (EF680300). The specific pathogenic amino-acid sequence "YXXM" was found in the TM district, similar to the exogenous JSRV: this gene has been reported to be oncogenic. This is the first report of the complete genomic sequence of the exogenous JSRV from Xinjiang, and could lay the foundation for study of the biological characteristics and pathogenic mechanisms of the pulmonary adenomatosis virus in sheep.
authors.forename: Sufang
authors.initials: S
authors.lastname: Yang
authors.forename: Tian
authors.initials: T
authors.lastname: Liang
authors.forename: Qingliang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Dianqing
authors.initials: D
authors.lastname: Zhang
authors.lastname: Si Junqiang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.forename: Jinliang
authors.initials: J
authors.lastname: Sheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D016679:Genome, Viral; D022141:Jaagsiekte sheep retrovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008168:Lung / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011648:Pulmonary Adenomatosis, Ovine / Q000473:pathology / Q000821:virology*; D012756:Sheep; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence
chemical_list: D014764:Viral Proteins