title: [Expression of microRNA-29b in mice with amyotrophic lateral sclerosis].
abstract: OBJECTIVE
  To investigate microRNA-29b (miR-29b) expression in cerebral cortex, spinal cord, fore limb muscle, and serum of SOD1-G93A amyotrophic lateral sclerosis (ALS) mice, and to identify the biomarker and to assess diagnostic values for ALS.
  
  
  METHODS
  Cerebral cortex, spinal cord, fore limb muscle and serum from 16 SOD1-G93A ALS mice and 16 wild-type mice were taken and then microRNA extracted, detecting the expression of miR-29b by real-time quantitative polymerase chain reaction (RT-qPCR). The diagnostic performance of miR-29b for ALS was estimated by the receiver operating characteristic (ROC) curve.
  
  
  RESULTS
  The results from the validation indicated that the differences in miR-29b between the cerebral cortex of SOD1-G93A ALS and the healthy control subjects were statistically significant (P=0.001). Meanwhile, the expressions 8, 12, and 16 weeks later were higher than those of the controls (ALS vs.
  
  
  METHODS
  8 weeks, P=0.044; 12 weeks, P=0.018; 16 weeks, P=0.045). When the relative expression level of miR-29b was used to diagnose ALS in SOD1-G93A ALS mice, the area under the ROC (area under the curve, AUC) was 0.885, if the diagnostic threshold was set at 0.185 6, the sensitivity and specificity were 92.9% and 71.4%.
  
  
  CONCLUSIONS
  miR-29b may act as medical monitoring indices of ALS in early time.
authors.affiliation: Department of Neurology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cai
authors.affiliation: Department of Neurology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Fan
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C547014:MIRN29 microRNA, mouse; D035683:MicroRNAs; D013482:Superoxide Dismutase