title: A metabolomic study using HPLC-TOF/MS coupled with ingenuity pathway analysis: Intervention effects of Rhizoma Alismatis on spontaneous hypertensive rats.
abstract: Rhizoma Alismatis, an important crude herb component in traditional Chinese medicine, has been commonly used for treating hypertension, but its mechanism has not been clarified in level of metabolic. Therefore, in the present work, metabolomics study coupled with ingenuity pathway analysis (IPA) have been performed to explore the potential mechanism in anti-hypertensive effect of Rhizoma Alismatis. Serum samples from SHRs and WKYs were analyzed with the use of high performance liquid chromatography coupled with time of flight mass spectrometry detection (HPLC-TOF/MS) in positive polarity. The obtained data sets were statistically analyzed using univariate and multivariate statistical analyses including the principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) for pattern recognition and selection of significant metabolites. 12 potential biomarkers in rat serum were screened out from HMDB, METLIN and KEGG databases. Furthermore, IPA was introduced and Glycerophospholipid metabolism and Purine metabolism were filtered out as potential target pathways. This metabolomic approach coupled with IPA provided a feasible way to understand the therapeutic mechanism of Rhizoma Alismatis on spontaneous hypertensive rats.
authors.affiliation: Traditional Chinese Medicine Clinical Research Base for Hypertension of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Wenhua Xi Road, Jinan 250011, China; Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Chu
authors.affiliation: Experimental Center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Ju
authors.affiliation: Traditional Chinese Medicine Clinical Research Base for Hypertension of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Wenhua Xi Road, Jinan 250011, China; Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China. Electronic address: yunlun.lee@hotmail.com.
authors.forename: Yunlun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Experimental Center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Gong
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Yang
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis / Q000379:methods*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D027343:Rhizome; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal
keywords: High performance liquid chromatography coupled with time of flight mass spectrometry; Hypertension; Metabolomics; Rhizoma Alismatis; Traditional Chinese medicine