title: Heat induced aggregation of gold nanorods for rapid visual detection of lysozyme.
abstract: Gold nanorods have been nominated as propitious candidates for nanobiodiagnostic applications. Herein, a technique has been introduced for rapid visual detection of lysozyme, as its high level of excretion in biological fluids is a characteristic sign of leukemia and kidney disorders. Gold nanorods were biofunctionalized with lysozyme aptamer and characterized with UV-Visible and FTIR spectroscopy, zeta potential analyzer and transmission electron microscopy. Exposure of the nanoprobe to nano molar levels of lysozyme (20 nmol l(-1)) lead to dictated aggregation of the nanostructures at ambient temperature; which was significantly improved by heat induced morphological perturbations and rapid detection by the naked eye (down to pico molar level). Qualitative analysis of Acute myeloid leukemia, Acute lymphocytic leukemia and Lymphoma blood serums showed sensitivity and specificity of the fabricated aptasensor under both temperature conditions. This report encourages utilization of heat-induced aggregation of gold nanorods as a promising nanodiagnostic technique for the emerging nanotechnologies.
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Tohidi Moghadam
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: ranjbarb@modares.ac.ir.
authors.forename: Bijan
authors.initials: B
authors.lastname: Ranjbar
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007938:Leukemia / Q000097:blood / Q000201:enzymology; D057230:Limit of Detection; D009113:Muramidase / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry*; D009994:Osmolar Concentration; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D014867:Water; D006046:Gold; D009113:Muramidase
keywords: Aptasensor; Gold nanorods; Lab-in-a-vial; Morphological perturbations; Surface plasmon resonance