title: Identification of Vitis vinifera L. grape berry skin color mutants and polyphenolic profile.
abstract: A germplasm set of twenty-five grapevine accessions, forming eleven groups of possible berry skin color mutants, were genotyped with twelve microsatellite loci, being eleven of them identified as true color mutants. The polyphenolic profiling of the confirmed mutant cultivars revealed a total of twenty-four polyphenols, comprising non-colored compounds (phenolic acids, flavan-3-ols, flavonols and a stilbene) and anthocyanins. Results showed differences in the contribution of malvidin-3-O-glucoside to the characteristic Pinot Noir anthocyanins profile. Regarding the two Pique-Poul colored variants, the lighter variant was richer than the darker one in all classes of compounds, excepting anthocyanins. In Moscatel Galego Roxo the F3'H pathway seems to be more active than F3'5'H, resulting in higher amounts of cyanidin, precursor of the cyanidin derivatives. As far as we are aware, this is the first time that a relationship between the content of polyphenolic compounds is established in groups of grape berry skin color mutant cultivars.
authors.affiliation: Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicas (CITAB), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal; Centro de Biotecnología y Genómica de Plantas (CBGP), Campus de Montegancedo, Autovía M40, km38, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ferreira
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors.forename: Fátima
authors.initials: F
authors.lastname: Fernandes
authors.affiliation: Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.
authors.forename: Olinda
authors.initials: O
authors.lastname: Pinto-Carnide
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.affiliation: Centro de Química de Vila Real (CQ-VR), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal.
authors.forename: Virgílio
authors.initials: V
authors.lastname: Falco
authors.affiliation: Dpto. de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitária s/n, 28040 Madrid, Spain.
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Martín
authors.affiliation: Dpto. de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitária s/n, 28040 Madrid, Spain.
authors.forename: Jesús María
authors.initials: JM
authors.lastname: Ortiz
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Campus de Montegancedo, Autovía M40, km38, 28223 Pozuelo de Alarcón, Madrid, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Arroyo-García
authors.affiliation: REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
authors.affiliation: Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicas (CITAB), Universidade de Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal. Electronic address: icastro@utad.pt.
authors.forename: Isaura
authors.initials: I
authors.lastname: Castro
mesh_terms: D003116:Color; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D059808:Polyphenols / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*
chemical_list: D010636:Phenols; D059808:Polyphenols
keywords: Anthocyanins; Caftaric acid (PubChem CID: 6440397); Catechin (PubChem CID: 73160); Epigallocatechin gallate (PubChem CID: 65064); Gallic acid (PubChem CID: 370); Malvidin-3-O-glucoside (PubChem CID: 443652); Malvidin-3-O-p-coumaroylglucoside (PubChem CID: 44256988); Microsatellites; Non-colored phenolics; Peonidin-3-O-p-coumaroylglucoside (PubChem CID: 44256849); Quercetin-3-O-galactoside (PubChem CID: 5281643); Quercetin-3-O-glucoside (PubChem CID: 25203368); Resveratrol-3-O-glucoside (PubChem CID: 25579167); Skin color mutants; Vitis vinifera L.