title: Ectomycorrhizae of Tuber huidongense and T. liyuanum with Castanea mollissima and Pinus armandii.
abstract: Tuber huidongense and T. liyuanum are common commercial white truffles in China that belong to the Rufum and Puberulum groups of the genus Tuber, respectively. Their mycorrhizae were successfully synthesized with two native trees--Castanea mollissima and Pinus armandii--under greenhouse conditions. The identities of the mycorrhizae were confirmed through internal transcribed spacer (ITS) sequence analyses, and their morphological characteristics were described. All of the obtained mycorrhizae have an interlocking pseudoparenchymatous mantle, which is a typical feature of truffle mycorrhizae. The mycorrhizae of T. huidongense on the two trees have hyaline branched emanating hyphae, similar to the documented mycorrhizae of the Rufum group. The unramified, spiky, and hyaline cystidia on the mycorrhizae of T. liyuanum with both C. mollissima and P. armandii further confirmed that this characteristic is constant for the mycorrhizae of the Puberulum group. The successful mycorrhizal syntheses on the two nut-producing trees will be of economic importance in the cultivation of the two truffles.
authors.affiliation: College of Resource and Environment, Yunnan Agricultural University, Kunming, 650100, People's Republic of China.|Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
authors.forename: Shan-Ping
authors.initials: SP
authors.lastname: Wan
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Yu
authors.affiliation: College of Resource and Environment, Yunnan Agricultural University, Kunming, 650100, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.affiliation: New Zealand Institute for Plant and Food Research Limited, Christchurch, 8140, New Zealand.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China.
authors.forename: Pei-Gui
authors.initials: PG
authors.lastname: Liu
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China. xhwang@mail.kib.ac.cn.
authors.forename: Xiang-Hua
authors.initials: XH
authors.lastname: Wang
authors.affiliation: College of Resource and Environment, Yunnan Agricultural University, Kunming, 650100, People's Republic of China. zhengyi-64@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000166:cytology* / Q000235:genetics; D001483:Base Sequence; D002681:China; D004275:DNA, Ribosomal; D029962:Fagaceae / Q000382:microbiology*; D025301:Hyphae / Q000254:growth & development; D038821:Mycorrhizae / Q000145:classification* / Q000166:cytology* / Q000235:genetics; D010802:Phylogeny; D028223:Pinus / Q000382:microbiology*; D036226:Seedlings / Q000382:microbiology; D012639:Seeds / Q000382:microbiology; D017421:Sequence Analysis; D014197:Trees / Q000382:microbiology
chemical_list: D004275:DNA, Ribosomal
keywords: Mycorrhization; Puberulum group; Rufum group; White truffle