title: Decreased expression levels of cell cycle regulators and matrix metalloproteinases in melanoma from RET-transgenic mice by single irradiation of non-equilibrium atmospheric pressure plasmas.
abstract: Since effective therapies for melanoma with BRAF(V600E) mutation are being developed, interest has been shown in the development of therapies for melanoma without BRAF(V600E) mutation. Recently, interest has also been shown in medical application of non-nequilibrium atmospheric pressure plasmas (NEAPPs). We previously suggested that repeated NEAPP irradiation to spontaneously developed benign melanocytic tumors in RFP-RET-transgenic mice (RET-mice) not only suppresses tumor growth but also prevents malignant transformation. In this study, we first confirmed that transcript expression levels of tumor growth regulators (CyclinD1, D2, E1, E2, G2 and PCNA but not CyclinG1) and tumor invasion regulators [Matrix metalloproteinase (MMP)-2, -9 and -14 and melanoma cell adhesion molecule (MCAM)] in melanomas were significantly higher than those in benign melanocytic tumors in RET-mice. We then showed that transcript expression levels of CyclinE1, G1 and G2 and MMP-2 and -9 in melanomas from RET-mice were significantly decreased by single NEAPP irradiation, whereas transcript expression levels of CyclinD1, D2, E2, PCNA, MCAM and MMP-14 were comparable in untreated and NEAPP-treated melanomas. Since no Braf(V600E) mutation melanomas have been found in RET-mice, our results suggest that single NEAPP irradiation is a potential therapeutic tool for melanoma without BRAF(V600E) mutation through modulation of the expression levels of tumor growth and invasion regulators.
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan ; Department of Biomedical Sciences, Unit of Environmental Health Sciences, College of Life and Health Sciences, Chubu University Kasugai, Aichi 487-8501, Japan.
authors.forename: Machiko
authors.initials: M
authors.lastname: Iida
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Omata
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Nakano
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan ; Department of Biomedical Sciences, Unit of Environmental Health Sciences, College of Life and Health Sciences, Chubu University Kasugai, Aichi 487-8501, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yajima
authors.affiliation: Department of Pathology, Nagoya Daini Red Cross Hospital Showa-ku, Nagoya, Aichi 466-8650, Japan.
authors.forename: Toyonori
authors.initials: T
authors.lastname: Tsuzuki
authors.affiliation: Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishikawa
authors.affiliation: Department of Electrical Engineering and Computer Science, Graduate School of Engineering, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Hori
authors.affiliation: Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan ; Department of Biomedical Sciences, Unit of Environmental Health Sciences, College of Life and Health Sciences, Chubu University Kasugai, Aichi 487-8501, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
mesh_terms: D000818:Animals; D051929:CD146 Antigen / Q000235:genetics / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000532:radiotherapy*; D051379:Mice; D008822:Mice, Transgenic; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism / Q000532:radiotherapy*
chemical_list: D051929:CD146 Antigen; D016213:Cyclins; D020782:Matrix Metalloproteinases
keywords: Melanoma; invasion; non-equilibrium atmospheric pressure plasmas; tumor growth