title: Label-free Detection of Zn(2+) Based on G-quadruplex.
abstract: Herein, we developed a sensing strategy for the label-free detection of Zn(2+) based on G-quadruplex. In the absence of Zn(2+), there was a fluorescence enhancement of thioflavin T by interaction with human telomere sequence. On the addition of Zn(2+), Zn(2+) induced a more compact G-quadruplex structure to release thioflavin T, resulting in a fluorescence decrease. This simple "mix-then-detect" method gave the detection limit of 0.91 μM with linear dynamic ranges from 0 to 10 μM. Because it does not require the use of expensive and unstable DNAzyme systems, or need synthesis and modification of nanomaterials, this label-free biosensor is simple, fast, cost-effective and applicable for real samples taken from lake water.
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Shen
authors.forename: Kunchi
authors.initials: K
authors.lastname: Zhang
authors.forename: Jiming
authors.initials: J
authors.lastname: Hu
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D052160:Benzothiazoles; D015374:Biosensing Techniques / Q000379:methods*; D054856:G-Quadruplexes; D006801:Humans; D057230:Limit of Detection; D016615:Telomere / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D013844:Thiazoles; C009462:thioflavin T; D015032:Zinc