title: Methicillin-Resistant Staphylococcus aureus Grown on Vancomycin-Supplemented Screening Agar Displays Enhanced Biofilm Formation.
abstract: Brain heart infusion agar containing 3 mg/liter vancomycin (BHI-V3) was used to screen for heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA). There was markedly greater biofilm formation by isolates that grew on BHI-V3 than by strains that did not grow on BHI-V3. Increased biofilm formation by hVISA may be mediated by FnbA- and polysaccharide intercellular adhesin-dependent pathways, and upregulation of atlA and sarA may also contribute to enhanced biofilm formation by hVISA upon prolonged exposure to vancomycin.
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Chang
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China Bengbu Medical College, Bengbu, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Ding
authors.affiliation: School of Medicine, Shandong University, Jinan, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China Bengbu Medical College, Bengbu, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China.
authors.forename: Huaiwei
authors.initials: H
authors.lastname: Lu
authors.affiliation: Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei, China.
authors.forename: Jilong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Clinical Laboratory, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China xiaolingma@126.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ma
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013203:Staphylococcal Infections / Q000382:microbiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D012333:RNA, Messenger; C088021:SarA protein, bacterial; D015534:Trans-Activators; D014640:Vancomycin; D000362:Agar; D008712:Methicillin