title: Surface vs. solution hybridization: effects of salt, temperature, and probe type.
abstract: Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.
authors.affiliation: Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA. rl1306@nyu.edu.
authors.forename: Wanqiong
authors.initials: W
authors.lastname: Qiao
authors.affiliation: Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA. rl1306@nyu.edu.
authors.forename: Hao-Chun
authors.initials: HC
authors.lastname: Chiang
authors.affiliation: Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA. rl1306@nyu.edu.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.affiliation: Dept. of Chemical & Biomolecular Engineering, NYU Polytechnic School of Engineering, 6 MetroTech Center, Brooklyn, NY 11201, USA. rl1306@nyu.edu.
authors.forename: Rastislav
authors.initials: R
authors.lastname: Levicky
mesh_terms: D015335:Molecular Probes; D012492:Salts / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics
chemical_list: D015335:Molecular Probes; D012492:Salts; D012996:Solutions