title: Contribution of Invariant Natural Killer T Cells to Skin Wound Healing.
abstract: In the present study, we determined the contribution of invariant natural killer T (iNKT) cells to the skin wound healing process. In iNKT cell-deficient (Jα18KO) mice lacking iNKT cells, wound closure was significantly delayed compared with wild-type mice. Collagen deposition, expression of α-smooth muscle actin and CD31, and wound breaking strength were significantly attenuated in Jα18KO mice. The adoptive transfer of liver mononuclear cells from wild-type but not from Jα18KO or interferon (IFN)-γ gene-disrupted (IFN-γKO) mice resulted in the reversal of this impaired wound healing in Jα18KO mice. IFN-γ expression was induced in the wounded tissues, which was significantly decreased at 6, 12, and 24 hours, but increased on day 3 after wounding in Jα18KO mice. The main source of the late-phase IFN-γ production in Jα18KO mice were neutrophils rather than NK cells and T cells. Administration of α-galactosylceramide, an activator of iNKT cells, resulted in the acceleration of wound healing on day 3 in wild-type mice. This effect was not observed in IFN-γKO mice. These results indicate that iNKT cells play important roles in wound healing. The iNKT cell-induced IFN-γ production may regulate the wound healing process in the early phase.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tanno
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Masae
authors.initials: M
authors.lastname: Ritsu
authors.affiliation: Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Kanno
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Kamimatsuno
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takagi
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Miyasaka
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Imai
authors.affiliation: Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Maruyama
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan. Electronic address: tachi@med.tohoku.ac.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tachi
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D005699:Galactosylceramides / Q000494:pharmacology; D015870:Gene Expression / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000187:drug effects / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000276:immunology / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects / Q000276:immunology* / Q000502:physiology
chemical_list: D005699:Galactosylceramides; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; C493154:alpha-galactosylceramide; D015847:Interleukin-4; D007371:Interferon-gamma; D003094:Collagen