title: The Analysis of a Microbial Community in the UV/O3-Anaerobic/Aerobic Integrated Process for Petrochemical Nanofiltration Concentrate (NFC) Treatment by 454-Pyrosequencing.
abstract: In this study, high-throughput pyrosequencing was applied on the analysis of the microbial community of activated sludge and biofilm in a lab-scale UV/O3- anaerobic/aerobic (A/O) integrated process for the treatment of petrochemical nanofiltration concentrate (NFC) wastewater. NFC is a type of saline wastewater with low biodegradability. From the anaerobic activated sludge (Sample A) and aerobic biofilm (Sample O), 59,748 and 51,231 valid sequence reads were obtained, respectively. The dominant phylotypes related to the metabolism of organic compounds, polycyclic aromatic hydrocarbon (PAH) biodegradation, assimilation of carbon from benzene, and the biodegradation of nitrogenous organic compounds were detected as genus Clostridium, genera Pseudomonas and Stenotrophomonas, class Betaproteobacteria, and genus Hyphomicrobium. Furthermore, the nitrite-oxidising bacteria Nitrospira, nitrite-reducing and sulphate-oxidising bacteria (NR-SRB) Thioalkalivibrio were also detected. In the last twenty operational days, the total Chemical Oxygen Demand (COD) and Total Organic Carbon (TOC) removal efficiencies on average were 64.93% and 62.06%, respectively. The removal efficiencies of ammonia nitrogen and Total Nitrogen (TN) on average were 90.51% and 75.11% during the entire treatment process.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China; Tianjin Waterworks Group Co., Ltd., Tianjin, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: He
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: School of Energy and Civil Engineering, Harbin University of Commerce, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D044822:Biodiversity; D002849:Chromatography, Gas; D004785:Environmental Pollutants / Q000032:analysis; D005374:Filtration; D036103:Nanotechnology; D009930:Organic Chemicals / Q000032:analysis; D010126:Ozone / Q000032:analysis*; D010802:Phylogeny; D012111:Residence Characteristics; D017422:Sequence Analysis, DNA; D014466:Ultraviolet Rays; D014865:Waste Disposal, Fluid
chemical_list: D004785:Environmental Pollutants; D009930:Organic Chemicals; D010126:Ozone