title: Development of an Infarct Volume Algorithm to Correct for Brain Swelling After Ischemic Stroke in Rats.
abstract: The primary measure for experimental stroke studies, infarct volume, can be affected by brain swelling. The algorithm by Lin et al. was developed to correct for brain swelling, however, the correction is not adequate. This chapter presents a new infarct volume algorithm that more appropriately corrects for brain hemisphere volume changes (swelling and stunted growth). Fifty-one adult rats were sacrificed 24 h after middle cerebral artery occlusion (MCAO). Forty-four P10 rat pups were sacrificed 48 h after hypoxia-ischemia (HI). Infarct volumes for 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) stained brains were calculated using our algorithm and that of Lin and colleagues. For MCAO animals, the algorithm of Lin et al. computed smaller infarct volumes than those of our algorithm. For HI animals, Lin et al.'s algorithm's infarct volumes were greater than those of our algorithm. For sham animals, Lin et al.'s algorithm's computed infarct volumes were significantly different from those of our algorithm. Our algorithm produces a more robust estimation of infarct volume than Lin et al.'s algorithm because the effects of ipsilesional hemisphere volume changes are minimized. Herein, our algorithm yields an infarct volume that better corrects for brain swelling and stunted brain growth compared with the algorithm of Lin et al.
authors.affiliation: Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA, 92350, USA.
authors.forename: Devin W
authors.initials: DW
authors.lastname: McBride
authors.affiliation: Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA, 92350, USA.
authors.forename: Jiping
authors.initials: J
authors.lastname: Tang
authors.affiliation: Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA, 92350, USA. jhzhang@llu.edu.|Department of Neurosurgery, Loma Linda University School of Medicine, Loma Linda, CA, USA. jhzhang@llu.edu.
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000473:pathology*; D001929:Brain Edema / Q000473:pathology*; D020520:Brain Infarction / Q000473:pathology*; D004195:Disease Models, Animal; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke
keywords: Brain swelling; Experimental stroke; Infarct volume; Middle cerebral artery occlusion