title: Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian).
abstract: Lotus is an aquatic plant with high nutritional, ornamental and medical values. Its callus formation is crucial for germplasm innovation by genetic transformation. In this study, embryogenic callus was successfully induced on appropriate medium using cotyledons at 12days after pollination as explants. To dissect cellular dedifferentiation and callus formation processes at the proteome level, cotyledons before and tissues from 10 to 20days after induction were sampled for shotgun proteomic analysis. By applying multivariate statistics 91 proteins were detected as differentially regulated, and sorted into 6 functional groups according to MapMan ontology analysis. Most of these proteins were implicated in various metabolisms, demonstrating that plant cells underwent metabolism reprogramming during callus induction. 14.3% proteins were associated with stress and redox, indicating that the detached explants were subjected to a variety of stresses; 13.2% were cell and cell wall-related proteins, suggesting that these proteins played important roles in rapid cell division and proliferation. Some proteins were further evaluated at the mRNA levels by quantitative reverse transcription PCR analysis. In conclusion, the results contributed to further deciphering of molecular processes of cellular dedifferentiation and callus formation, and provided a reference data set for the establishment of transgenic transformation in lotus.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Moshan 430074, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Ecogenomics and Systems Biology, University of Vienna, Althanstr. 14, Vienna 1090, Austria.
authors.forename: Palak
authors.initials: P
authors.lastname: Chaturvedi
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Moshan 430074, China.
authors.forename: Jinlei
authors.initials: J
authors.lastname: Fu
authors.affiliation: College of Life science and technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Department of Ecogenomics and Systems Biology, University of Vienna, Althanstr. 14, Vienna 1090, Austria.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Weckwerth
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences, Moshan 430074, China. Electronic address: yangpf@wbgcas.cn.
authors.forename: Pingfang
authors.initials: P
authors.lastname: Yang
mesh_terms: D054337:Cell Dedifferentiation / Q000502:physiology*; D031653:Nelumbo / Q000166:cytology* / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018547:Plant Stems / Q000378:metabolism*; D010941:Plant Tumors; D020543:Proteome / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Callus induction; Cotyledons; Lotus; Quantitative proteomics