title: MiR-1271 Inhibits Ovarian Cancer Growth by Targeting Cyclin G1.
abstract: BACKGROUND
  Ovarian cancer is the most lethal gynecological malignant cancer in the female genital system. The dysfunction of miRNA contributes to ovarian cancer development.
  
  
  METHODS
  The miR-1271 level in ovarian cancer tissues and cells was assayed by qRT-PCR. The miR-1271 expression in cells was overexpressed by miRNA-mimic transfection and reduced by miRNA-antisense-oligonucleotide (ASO) transfection. Cell proliferation was analyzed by an MTT assay. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. The protein level was assayed by Western blotting.
  
  
  RESULTS
  The ovarian cancer tissue and cell lines showed low levels of miR-1271. Low levels of miR-1271 in ovarian cancer tissues were correlated with a low rate of patient survival, and the overexpression of miR-1271 inhibited the proliferation of ovarian cancer cells. The 3' UTR of cyclin G1 (CCNG1) was targeted by miR-1271.
  
  
  CONCLUSIONS
  Low levels of miR-1271 in ovarian cancer tissues promoted cancer cell growth. MiR-1271 may be a new predictor of prognosis in ovarian cancer. MiR-1271 exerted its role by targeting CCNG1.
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Lihong
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Rao
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yueyang, Hunan, China (mainland).
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Huiqin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Changju
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Gynaecology and Obstetrics, People's Hospital of Yuxi, Yuxi, Yunnan, China (mainland).
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D000465:Algorithms; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D049109:Cell Proliferation; D056766:Cyclin G1 / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism*; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D020413:3' Untranslated Regions; D015703:Antigens, CD; C534702:CCNG1 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D056766:Cyclin G1; C556309:MIRN1271 microRNA, human; D035683:MicroRNAs