title: Intranasal Osteopontin for Rodent Germinal Matrix Hemorrhage.
abstract: Germinal matrix hemorrhage (GMH) is the most common and devastating neurological problem of premature infants. Current treatment is largely ineffective and GMH has been nonpreventable. Osteopontin (OPN) is an endogenous protein that has been shown to be neuroprotective, however, it has not been tested in GMH. P7 neonatal rats were subjected to stereotactic ganglionic eminence collagenase infusion. Groups were as follows: (1) sham, (2) GMH + vehicle, (3) GMH + intranasal OPN. Seventy-two hours later, the animals were evaluated using righting reflex, blood-brain barrier (BBB) permeability by Evans blue dye leakage, brain water content, and hemoglobin assay. Intranasal OPN improved outcomes after GMH by attenuation of brain swelling, BBB function, re-bleeding, and neurological outcomes. OPN may play an important role in enhancing neuroprotective brain signaling following GMH. These observed effects may offer novel possibilities for therapy in this patient population.
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA.
authors.forename: Jay
authors.initials: J
authors.lastname: Malaguit
authors.affiliation: Departments of Neonatology, School of Medicine, Loma Linda, CA, USA.
authors.forename: Darlene
authors.initials: D
authors.lastname: Casel
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Dixon
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA.
authors.forename: Desislava
authors.initials: D
authors.lastname: Doycheva
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA.
authors.forename: Jiping
authors.initials: J
authors.lastname: Tang
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA.|Neurosurgery, School of Medicine, Loma Linda, CA, USA.
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Division of Physiology and Pharmacology, School of Medicine, Loma Linda, CA, USA. tlekic@llu.edu.|Department of Physiology, Loma Linda University School of Medicine, 11041 Campus Street, Risley Hall Rm 129, Loma Linda, CA, 92354, USA. tlekic@llu.edu.
authors.forename: Tim
authors.initials: T
authors.lastname: Lekic
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000187:drug effects*; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism; D020300:Intracranial Hemorrhages / Q000150:complications / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D053495:Osteopontin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006454:Hemoglobins; D053495:Osteopontin
keywords: Germinal matrix hemorrhage; Hydrocephalus; Neonatal rats; Neurological dysfunction; Osteopontin; Stroke, experimental