title: Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients.
abstract: Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles ≥ 50%, compared to the group with relative proportion of sdLDL particles < 50%. Plasma resistin correlated positively with creatinine (r = 0.456, P < 0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R(2) = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Joksić
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Miron
authors.initials: M
authors.lastname: Sopić
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Spasojević-Kalimanovska
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Gojković
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Zeljković
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Vekić
authors.affiliation: Institute for Cardiovascular Diseases, Clinical Center of Serbia, Belgrade, Serbia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Andjelkovic
authors.affiliation: Institute for Cardiovascular Diseases, Clinical Center of Serbia, Belgrade, Serbia.|School of Medicine University of Belgrade, Belgrade, Serbia.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Kalimanovska-Oštrić
authors.affiliation: Department of Medical Biochemistry, Faculty of Pharmacy, Belgrade, Serbia.
authors.forename: Zorana
authors.initials: Z
authors.lastname: Jelić-Ivanović
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D008078:Cholesterol, LDL / Q000097:blood* / Q000737:chemistry*; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000000981:diagnostic imaging / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D052243:Resistin / Q000097:blood* / Q000235:genetics*
chemical_list: D008078:Cholesterol, LDL; D012333:RNA, Messenger; D052243:Resistin
keywords: coronary artery disease; gene expression; human; resistin; small dense LDL