title: Cafestol, a Bioactive Substance in Coffee, Stimulates Insulin Secretion and Increases Glucose Uptake in Muscle Cells: Studies in Vitro.
abstract: Diet and exercise intervention can delay or prevent development of type-2-diabetes (T2D), and high habitual coffee consumption is associated with reduced risk of developing T2D. This study aimed to test whether selected bioactive substances in coffee acutely and/or chronically increase insulin secretion from β-cells and improve insulin sensitivity in skeletal muscle cells. Insulin secretion from INS-1E rat insulinoma cells was measured after acute (1-h) and long-term (72-h) incubation with bioactive substances from coffee. Additionally, we measured uptake of radioactive glucose in human skeletal muscle cells (SkMC) after incubation with cafestol. Cafestol at 10(-8) and 10(-6) M acutely increased insulin secretion by 12% (p < 0.05) and 16% (p < 0.001), respectively. Long-term exposure to 10(-10) and 10(-8) M cafestol increased insulin secretion by 34% (p < 0.001) and 68% (p < 0.001), respectively. Caffeic acid also increased insulin secretion acutely and chronically. Chlorogenic acid, trigonelline, oxokahweol, and secoisolariciresinol did not significantly alter insulin secretion acutely. Glucose uptake in SkMC was significantly enhanced by 8% (p < 0.001) in the presence of 10(-8) M cafestol. This newly demonstrated dual action of cafestol suggests that cafestol may contribute to the preventive effects on T2D in coffee drinkers and be of therapeutic interest.
authors.affiliation: Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark.
authors.forename: Fredrik Brustad
authors.initials: FB
authors.lastname: Mellbye
authors.affiliation: Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark.
authors.forename: Per Bendix
authors.initials: PB
authors.lastname: Jeppesen
authors.affiliation: Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark.
authors.forename: Kjeld
authors.initials: K
authors.lastname: Hermansen
authors.affiliation: Department of Endocrinology and Internal Medicine, Aarhus University Hospital , Tage-Hansens Gade 2, 8000 Aarhus C Denmark.
authors.forename: Søren
authors.initials: S
authors.lastname: Gregersen
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002072:Butylene Glycols / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000494:pharmacology; D003069:Coffee / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000517:prevention & control*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000276:immunology / Q000378:metabolism / Q000493:pharmacokinetics*; D006168:Guinea Pigs; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism*
chemical_list: D000470:Alkaloids; D002072:Butylene Glycols; D002109:Caffeic Acids; D003069:Coffee; D004224:Diterpenes; D007328:Insulin; D017705:Lignans; D002726:Chlorogenic Acid; C009560:trigonelline; C053400:cafestol; D005947:Glucose; C060283:secoisolariciresinol