title: The association of paraoxonase 1 gene L55M polymorphism with the extent and severity of coronary artery disease in the Turkish population and its dependence on gender.
abstract: OBJECTIVE
  Coronary artery disease (CAD) is a common, complex, and progressive disorder characterized by the accumulation of lipids and fibrous elements in the arteries. It is one of the leading causes of death in industrialized nations. Oxidative modification of low-density lipoprotein (LDL) in the arterial wall plays an important role in the initiation and progression of atherosclerosis. Paraoxonase1 (PON1) is involved in lipid metabolism and is believed to protect LDL oxidation. In our study, we aimed to clarify the relationship between PON1 gene L55M polymorphism and the extent and severity of CAD.
  
  
  METHODS
  In total, 114 patients (54 males, mean age: 56.7 ± 12.0 years; 60 females, mean age: 55.7 ± 13.2 years) with stable angina or angina equivalent symptoms were enrolled in this prospective study. Cardiological evaluation was performed with electrocardiogram and transthoracic echocardiogram. The presence of hypertension, dyslipidemia, diabetes, and smoking status were ascertained. The patients were grouped according to their Gensini scores and gender. Genetic analysis of the PON1 gene L55M polymorphism was performed by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  We determined that the LL genotype was more prevalent in patients with Gensini score higher than or equal to 20 (p=0.026) and that this correlated with severe atherosclerotic coronary artery lesions in both gender groups, reaching a statistical significance in the female subjects (p=0.038).
  
  
  CONCLUSIONS
  It was thought that the PON1 gene L55M polymorphism plays a significant role in CAD progression, especially in females.
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Kırıkkale University; Kırıkkale-Turkey. dsayin@yahoo.com.
authors.forename: Derya Beyza
authors.initials: DB
authors.lastname: Sayın Kocakap
authors.forename: Mehmet Tolga
authors.initials: MT
authors.lastname: Doğru
authors.forename: Vedat
authors.initials: V
authors.lastname: Şimşek
authors.forename: Feryal
authors.initials: F
authors.lastname: Çabuk
authors.forename: Nesligül
authors.initials: N
authors.lastname: Yıldırım
authors.forename: Yunus
authors.initials: Y
authors.lastname: Çelik
authors.forename: Sibel
authors.initials: S
authors.lastname: Alyılmaz Bekmez
authors.forename: Solmaz
authors.initials: S
authors.lastname: Erdem
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics*; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D004562:Electrocardiography; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D012307:Risk Factors; D012720:Severity of Illness Index; D012737:Sex Factors; D014421:Turkey; D044465:White People / Q000235:genetics
chemical_list: D043303:Aryldialkylphosphatase; C468406:PON1 protein, human