title: The quorum-sensing inhibiting effects of stilbenoids and their potential structure-activity relationship.
abstract: Stilbenoids, known an important phytoalexins in plants, were renowned for their beneficial effects on cardiovascular, neurological and hepatic systems. In the present study, quorum sensing inhibition activity of ten stilbenoids were tested using Chromobacterium violaceum CV026 as the bio-indicator strain and the structure-activity relationship was also investigated. Among them, resveratrol (1), piceatannol (2) and oxyresveratrol (3) showed potential anti-QS activities. At the sub-MIC concentrations, 1-3 demonstrated a statistically significant reduction of violacein in C. violaceum CV026 in a concentration dependent manner. Furthermore, the effects of 1-3 on QS regulated virulence factors in Pseudomonas aeruginosa PAO1 were also evaluated. Our results showed that the stilbenoids 1-3 can markedly decreased the production of pyocyanin and swarming motility of P. aeruginosa PAO1. Further transcriptome analyses showed that 1-3 suppressed the expression of QS-induced genes: lasR, lasI, rhlR and rhlI.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Ji-Yang
authors.initials: JY
authors.lastname: Sheng
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Tong-Tong
authors.initials: TT
authors.lastname: Chen
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Tan
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address: aiqunj302@mail.njust.edu.cn.
authors.forename: Ai-Qun
authors.initials: AQ
authors.lastname: Jia
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002861:Chromobacterium / Q000187:drug effects / Q000235:genetics; D005798:Genes, Bacterial / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics; D011710:Pyocyanine / Q000037:antagonists & inhibitors; D053038:Quorum Sensing / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D013267:Stilbenes; C034912:puag-haad; C041525:3,3',4,5'-tetrahydroxystilbene; D011710:Pyocyanine
keywords: Chromobacterium violaceum CV026; Pseudomonas aeruginosa PAO1; Quorum sensing inhibitors; Stilbenoids