title: Probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum modulates immunoglobulin levels and cytokines expression in whey proteins sensitised mice.
abstract: BACKGROUND
  Cow milk allergy is the most common food allergy in children. So far, no effective treatment is available to prevent or cure food allergy. This study investigated whether orally administrated probiotics could suppress sensitisation in whey proteins (WP)-induced allergy mouse model. Two types of probiotic Dahi were prepared by co-culturing Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC-86 and Lactococcus lactis ssp. lactis biovar diacetylactis NCDC-60) along with selected strain of Lactobacillus acidophilus LaVK2 and Bifidobacterium bifidum BbVK3. Mice were fed with probiotic Dahi (La-Dahi and LaBb-Dahi) from 7 days before sensitisation with WP, respectively, in addition to milk protein-free basal diet, and control group received no supplements.
  
  
  RESULTS
  Feeding of probiotic Dahi suppressed the elevation of whey proteins-specific IgE and IgG response of WP-sensitised mice. In addition, sIgA levels were significantly (P < 0.001) increased in intestinal fluid collected from mice fed with La-Dahi. Production of T helper (Th)-1 cell-specific cytokines, i.e. interferon-γ (IFN-γ), interleukin (IL)-12, and IL-10 increased, while Th2-specific cytokines, i.e. IL-4 decreased in the supernatant of cultured splenocytes collected from mice fed with probiotic Dahi as compared to the other groups. Moreover, the splenic mRNA levels of IFN-γ, interleukin-10 were found to be significantly increased, while that of IL-4 decreased significantly in La-Dahi groups, as compared to control groups.
  
  
  CONCLUSIONS
  Results of the present study indicate that probiotic Dahi skewed Th2-specific immune response towards Th1-specific response and suppressed IgE in serum. Collectively, this study shows the potential use of probiotics intervention in reducing the allergic response to whey proteins in mice. © 2015 Society of Chemical Industry.
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Umesh Kumar
authors.initials: UK
authors.lastname: Shandilya
authors.affiliation: UIET-Kurukshetra University, Kurukshetra, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kapila
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, 132001, India.
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Kansal
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D000069985:Bifidobacterium bifidum / Q000276:immunology*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000178:diet therapy / Q000517:prevention & control; D007136:Immunoglobulins / Q000097:blood*; D007422:Intestines / Q000276:immunology; D007779:Lactobacillus acidophilus / Q000276:immunology*; D013294:Lactococcus lactis / Q000254:growth & development; D008297:Male; D051379:Mice; D016269:Milk Hypersensitivity / Q000188:drug therapy / Q000382:microbiology; D019936:Probiotics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D013154:Spleen / Q000276:immunology; D000067816:Whey Proteins / Q000276:immunology / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007136:Immunoglobulins; D012333:RNA, Messenger; D000067816:Whey Proteins
keywords: Bifidobacterium bifidum; Lactobacillus acidophilus; milk allergy; probiotics