title: Mutations in the Arabidopsis Lst8 and Raptor genes encoding partners of the TOR complex, or inhibition of TOR activity decrease abscisic acid (ABA) synthesis.
abstract: The Target of Rapamycin (TOR) kinase regulates essential processes in plant growth and development by modulation of metabolism and translation in response to environmental signals. In this study, we show that abscisic acid (ABA) metabolism is also regulated by the TOR kinase. Indeed ABA hormone level strongly decreases in Lst8-1 and Raptor3g mutant lines as well as in wild-type (WT) Arabidopsis plants treated with AZD-8055, a TOR inhibitor. However the growth and germination of these lines are more sensitive to exogenous ABA. The diminished ABA hormone accumulation is correlated with lower transcript levels of ZEP, NCED3 and AAO3 biosynthetic enzymes, and higher transcript amount of the CYP707A2 gene encoding a key-enzyme in abscisic acid catabolism. These results suggest that the TOR signaling pathway is implicated in the regulation of ABA accumulation in Arabidopsis.
authors.affiliation: L.N. Gumilyov Eurasian National University, 2 Satpayev Str, 010008, Astana, Kazakhstan; IJPB, UMR 1318 INRA-AgroParisTech, ERL CNRS 3559, Saclay Plant Sciences, F-78026, Versailles, France.
authors.forename: Alena
authors.initials: A
authors.lastname: Kravchenko
authors.affiliation: IJPB, UMR 1318 INRA-AgroParisTech, ERL CNRS 3559, Saclay Plant Sciences, F-78026, Versailles, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Citerne
authors.affiliation: IJPB, UMR 1318 INRA-AgroParisTech, ERL CNRS 3559, Saclay Plant Sciences, F-78026, Versailles, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jéhanno
authors.affiliation: L.N. Gumilyov Eurasian National University, 2 Satpayev Str, 010008, Astana, Kazakhstan.
authors.forename: Rakhmetkazhi I
authors.initials: RI
authors.lastname: Bersimbaev
authors.affiliation: Forage Improvement, AgResearch, Private Bag 11008, Palmerston North, 4442, New Zealand.
authors.forename: Bruce
authors.initials: B
authors.lastname: Veit
authors.affiliation: IJPB, UMR 1318 INRA-AgroParisTech, ERL CNRS 3559, Saclay Plant Sciences, F-78026, Versailles, France. Electronic address: cmeyer@versailles.inra.fr.
authors.forename: Christian
authors.initials: C
authors.lastname: Meyer
authors.affiliation: IJPB, UMR 1318 INRA-AgroParisTech, ERL CNRS 3559, Saclay Plant Sciences, F-78026, Versailles, France; Sorbonne Universités, UPMC Univ Paris 06, UFR927, 4 Place Jussieu, F-75252, Paris, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Leprince
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D017343:Genes, Plant; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D015398:Signal Transduction
chemical_list: D000040:Abscisic Acid; D029681:Arabidopsis Proteins; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; C512171:TOR protein, Arabidopsis
keywords: ABA metabolism; Abscisic acid (ABA); Arabidopsis thaliana; TOR kinase