title: Functional role of regulatory T cells in B cell lymphoma and related mechanisms.
abstract: B cell lymphoma (BCL) has a higher degree of malignancy and complicated pathogenic mechanism. Regulatory T cells (Treg cells) are known to exert certain immune suppression functions, in addition to immune mediating effects. Recent studies have revealed the role of Treg cells in pathogenesis and progression of multiple malignant tumors. This study therefore investigated the functional role and related mechanism of Treg cells in BCL. A cohort of thirty patients who were diagnosed with BCL in our hospital between January 2013 and December 2014. Another thirty healthy individuals were recruited. Peripheral blood mononuclear cells (PBMCs) were separated and analyzed for the ratio of CD4+/CD25+ Treg cells. The mRNA expression levels of Foxp3, transforming growth factor (TGF)-β1 and interleukin (IL)-10 genes were quantified by real-time PCR, while their serum levels were determined by enzyme-linked immunosorbent assay (ELISA). Meanwhile all laboratory indexes for patients were monitored during the complete remission (CR) stage. BCL patients significantly elevated ratio of CD4+/CD25+ Treg cells, which were decreased at CR stage. mRNA levels of Foxp3, TGF-β1 and IL-10, in addition to protein levels of TGF-β1 and IL-10 were potentiated in lymphoma patients but decreased in CR patients (P<0.05 in all cases). CD4+/CD25+ Treg cells exert immune suppressing functions in BCL via regulating cytokines, thereby facilitating the pathogenesis and progression of lymphoma.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Ruixiang
authors.initials: R
authors.lastname: Xia
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Zhenqi
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ni
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Anhui Medical University Hefei, China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D055815:Young Adult
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10
keywords: B cell lymphoma; Foxp3 gene; regulatory T cells; transforming growth factor-β1 interleukin-10