title: In vitro study of bioactivity of homemade tissue-engineered periosteum.
abstract: This study was aimed to evaluate in vitro bioactivity of tissue-engineered periosteum (TEP) which was fabricated by coupling either rabbits' MSCs or osteodifferentiated MSCs (O-MSCs) with porcine small intestinal submucosa (SIS), and that produced two kinds of TEPs (M1=MSCs+SIS and M2=Osteoblast+SIS). The cell adherence was observed under scanning electronic microscopy (SEM). Cell proliferation was measured by 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl-2 H-tetrazoliumbromide (MTT) test. In vitro osteogenetic potential of TEPs was evaluated kinetically at 0, 5, 10 and 15 d by measuring alkaline phosphatase (ALP) activity, and semi-quantitative assay of osteocalcin and collagen type I expression with Western blot. SEM indicated that either MSCs or O-MSCs could adhere and survive on SIS, but MTT test revealed that SIS was more preferable for O-MSCs than MSCs in proliferative aspect. In contrast, ALP activity, osteocalcin and collagen type I expression of M2 were higher than M1 in general. Kinetically, osteocalcin and collagen type I expression demonstrated a continuous increase, while ALP activity of both groups was displayed an early peak around the fifth day, followed a decrease trend. In conclusion, TEP, especially M2, has an active osteogenesis and is promising for in vivo bone defect reparation via a supposed biomimetic procedure of intramembranous ossification.
authors.affiliation: Orthopaedic Department, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500, China. Electronic address: bonezl@sina.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Nephrology, Shanghai ZhouPu Hospital, Shanghai 201318, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Orthopaedic Institute, The Second Hospital of Lanzhou University, Lanzhou 730030, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Yu
authors.affiliation: Orthopaedic Institute, The Second Hospital of Lanzhou University, Lanzhou 730030, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Orthopaedic Department, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Orthopaedic Department, Jinshan Branch of the Sixth People's Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 201500, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010521:Periosteum / Q000166:cytology* / Q000502:physiology*; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
keywords: Mesenchymal stem cells; Periosteum; Small intestinal submucosa; Tissue engineering