title: Quantitative determination of estrone by liquid chromatography-tandem mass spectrometry in subcutaneous adipose tissue from the breast in postmenopausal women.
abstract: Estrone is the most abundant estrogen after the menopause. We developed a liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for determination of estrone in adipose tissue. Subcutaneous adipose tissue from the breast was collected during elective surgery in postmenopausal women undergoing mastectomy for treatment of breast cancer (n=13) or reduction mammoplasty (controls, n=11). Homogenized adipose tissue was extracted with organic solvents and the estrone fraction was purified by LH-20 column chromatography from the excess of lipids. The concentration of estrone was analyzed by LC-MS/MS. The method was accurate with an intra-assay variation of 8% and an interassay variation of 10%. The median concentration of estrone in subcutaneous adipose tissue from the breast did not differ between breast cancer and control women, 920 pmol/kg and 890 pmol/kg, respectively. In breast cancer patients but not in the controls, breast adipose tissue estrone levels correlated positively with the serum estrone concentration. In conclusion, the new method provides a reliable means to measure estrone concentrations in adipose tissue in postmenopausal women.
authors.affiliation: Folkhälsan Research Center, 00290 Helsinki, Finland; University of Helsinki and Helsinki University Central Hospital, Heart and Lung Center, 00290 Helsinki, Finland. Electronic address: veera.vihma@helsinki.fi.
authors.forename: Veera
authors.initials: V
authors.lastname: Vihma
authors.affiliation: Folkhälsan Research Center, 00290 Helsinki, Finland; University of Helsinki and Helsinki University Central Hospital, Heart and Lung Center, 00290 Helsinki, Finland.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Folkhälsan Research Center, 00290 Helsinki, Finland; University of Helsinki and Helsinki University Central Hospital, Obstetrics and Gynecology, 00290 Helsinki, Finland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Savolainen-Peltonen
authors.affiliation: Helsinki University Central Hospital, HUSLAB, 00290 Helsinki, Finland.
authors.forename: Ursula
authors.initials: U
authors.lastname: Turpeinen
authors.affiliation: Helsinki University Central Hospital, HUSLAB, 00290 Helsinki, Finland.
authors.forename: Esa
authors.initials: E
authors.lastname: Hämäläinen
authors.affiliation: Helsinki University Central Hospital, Breast Surgery Unit, 00290 Helsinki, Finland.
authors.forename: Marjut
authors.initials: M
authors.lastname: Leidenius
authors.affiliation: Folkhälsan Research Center, 00290 Helsinki, Finland; University of Helsinki and Helsinki University Central Hospital, Obstetrics and Gynecology, 00290 Helsinki, Finland.
authors.forename: Tomi S
authors.initials: TS
authors.lastname: Mikkola
authors.affiliation: Folkhälsan Research Center, 00290 Helsinki, Finland; University of Helsinki and Helsinki University Central Hospital, Heart and Lung Center, 00290 Helsinki, Finland.
authors.forename: Matti J
authors.initials: MJ
authors.lastname: Tikkanen
mesh_terms: D000368:Aged; D015992:Body Mass Index; D002853:Chromatography, Liquid / Q000379:methods*; D004970:Estrone / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D042361:Mammary Glands, Human / Q000378:metabolism; D008875:Middle Aged; D017698:Postmenopause / Q000378:metabolism*; D015203:Reproducibility of Results; D050151:Subcutaneous Fat / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004970:Estrone
keywords: Adipose tissue; Breast cancer; Estrogen; Estrone; Liquid chromatography–tandem mass spectrometry; Menopause