title: Electric field-mediated growth of osteoblasts - the significant impact of dynamic flow of medium.
abstract: The endogenous electric field plays an important role in accomplishing various functions including communication with the brain and with different parts of the physiological system, wound healing, and cellular functions. Furthermore, the endogenous electric field can be modified using the external electric field to induce changes in cell functionality. Given that the cells grow in contact with the dynamic flow of blood and nutrients, the objective of the study is to elucidate the effect of media flow (dynamic conditions) on osteoblast functions at a pulsed DC (direct current) electric field of strength of 0.5-1 V cm(-1) and compared with the static conditions (no flow of media and in the presence of an electric field). The electric field provided a guiding cue to cells to move towards the cathode. An interesting aspect of the electric field was the migration of cells towards the cathode with the axis parallel to the direction of the electric field such that the lamellipodia was aligned. Furthermore, there was an absence of membrane blebbing or necrosis at the cathode. However, cell growth and expression of proteins (actin and vinculin) were higher than the anode. In contrast, at the anode, while the cells were healthy, the cell growth was less such that the expression of vinculin was relatively low together with less densely packed actin stress fibers. It is underscored that the biological functionality is favorably altered in the presence of an electrical field under dynamic conditions with a consequent effect on cell proliferation, growth, and expression level of prominent proteins, actin and vinculin.
authors.affiliation: Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA. dmisra2@utep.edu.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA. dmisra2@utep.edu.
authors.forename: K C
authors.initials: KC
authors.lastname: Nune
authors.affiliation: Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USA. dmisra2@utep.edu.
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D004558:Electric Stimulation; D006801:Humans; D010006:Osteoblasts / Q000737:chemistry*; D011554:Pseudopodia / Q000737:chemistry*; D016596:Vinculin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000199:Actins; D016596:Vinculin