title: The Arabidopsis Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage.
abstract: The structure of the cell wall has a major impact on plant growth and development, and alteration of cell wall structural components is often detrimental to biomass production. However, the molecular mechanisms responsible for these negative effects are largely unknown. Arabidopsis (Arabidopsis thaliana) plants with altered pectin composition because of either the expression of the Aspergillus niger polygalacturonase II (AnPGII; 35S:AnPGII plants) or a mutation in the QUASIMODO2 (QUA2) gene that encodes a putative pectin methyltransferase (qua2-1 plants), display severe growth defects. Here, we show that expression of Arabidopsis PEROXIDASE71 (AtPRX71), encoding a class III peroxidase, strongly increases in 35S:AnPGII and qua2-1 plants as well as in response to treatments with the cellulose synthase inhibitor isoxaben, which also impairs cell wall integrity. Analysis of atprx71 loss-of-function mutants and plants overexpressing AtPRX71 indicates that this gene negatively influences Arabidopsis growth at different stages of development, likely limiting cell expansion. The atprx71-1 mutation partially suppresses the dwarf phenotype of qua2-1, suggesting that AtPRX71 contributes to the growth defects observed in plants undergoing cell wall damage. Furthermore, AtPRX71 seems to promote the production of reactive oxygen species in qua2-1 plants as well as plants treated with isoxaben. We propose that AtPRX71 contributes to strengthen cell walls, therefore restricting cell expansion, during normal growth and in response to cell wall damage.
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Sara
authors.initials: S
authors.lastname: Raggi
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Alberto
authors.identifier: http://orcid.org/0000-0002-8452-3380
authors.initials: A
authors.lastname: Ferrarini
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Massimo
authors.identifier: http://orcid.org/0000-0002-7100-4581
authors.initials: M
authors.lastname: Delledonne
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Christophe
authors.identifier: http://orcid.org/0000-0003-1637-4042
authors.initials: C
authors.lastname: Dunand
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Philippe
authors.initials: P
authors.lastname: Ranocha
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Giulia
authors.identifier: http://orcid.org/0000-0002-1707-5418
authors.initials: G
authors.lastname: De Lorenzo
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.).
authors.forename: Felice
authors.identifier: http://orcid.org/0000-0001-8141-5352
authors.initials: F
authors.lastname: Cervone
authors.affiliation: Institute Pasteur-Fondazione Cenci Bolognetti and Dipartimento di Biologia e Biotecnologie "Charles Darwin," Sapienza Università di Roma, 00185 Rome, Italy (S.R., G.D.L., F.C., S.F.);Dipartimento di Biotecnologie, Università degli Studi di Verona, 37134 Verona, Italy (A.F., M.D.);Université de Toulouse, Université Paul Sabatier, Unité Mixte de Recherche 5546, Laboratoire de Recherche en Sciences Végétales, F-31326 Castanet-Tolosan, France (C.D., P.R.); andCentre National de la Recherche Scientifique, Unité Mixte de Recherche 5546, F-31326 Castanet-Tolosan, France (C.D., P.R.) simone.ferrari@uniroma1.it.
authors.forename: Simone
authors.identifier: http://orcid.org/0000-0002-1389-2090
authors.initials: S
authors.lastname: Ferrari
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020171:Botrytis / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D009154:Mutation; D010368:Pectins / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D010368:Pectins; D002482:Cellulose; D010544:Peroxidases; C000597916:Prx71 protein, Arabidopsis