title: A newly isolated Pseudomonas putida S-1 strain for batch-mode-propanethiol degradation and continuous treatment of propanethiol-containing waste gas.
abstract: Pseudomonas putida S-1 was isolated from activated sludge. This novel strain was capable of degrading malodorous 1-propanethiol (PT). PT degradation commenced with no lag phase by cells pre-grown in nutrition-rich media, such as Luria-Bertani (LB), and PT-contained mineral medium at specific growth rates of 0.10-0.19 h(-1); this phenomenon indicated the operability of a large-scale cell culture. A possible PT degradation pathway was proposed on the basis of the detected metabolites, including dipropyl disulfide, 3-hexanone, 2-hexanone, 3-hexanol, 2-hexanol, S(0), SO4(2-), and CO2. P. putida S-1 could degrade mixed pollutants containing PT, diethyl disulfide, isopropyl alcohol, and acetaldehyde, and LB-pre-cultured cells underwent diauxic growth. Waste gas contaminated with 200-400 mg/m(3) PT was continuously treated by P. putida S-1 pre-cultured in LB medium in a completely stirred tank reactor. The removal efficiencies exceeded 88% when PT stream was mixed with 200 mg/m(3) isopropanol; by contrast, the removal efficiencies decreased to 60% as the empty bed residence time was shortened from 40 s to 20 s.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: cdz@zjut.edu.cn.
authors.forename: Dong-Zhi
authors.initials: DZ
authors.lastname: Chen
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Sun
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Han
authors.affiliation: College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Jie-Xu
authors.initials: JX
authors.lastname: Ye
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Jian-Meng
authors.initials: JM
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D019149:Bioreactors; D016958:Pseudomonas putida / Q000302:isolation & purification* / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013438:Sulfhydryl Compounds / Q000378:metabolism*
chemical_list: D012722:Sewage; D013438:Sulfhydryl Compounds; C030762:propanethiol
keywords: Biodegradation; Continuous treatment; Metabolite; Propanethiol; Waste gas