title: Buffalo Cheese Whey Proteins, Identification of a 24 kDa Protein and Characterization of Their Hydrolysates: In Vitro Gastrointestinal Digestion.
abstract: Milk whey proteins are well known for their high biological value and versatile functional properties, characteristics that allow its wide use in the food and pharmaceutical industries. In this work, a 24 kDa protein from buffalo cheese whey was analyzed by mass spectrometry and presented homology with Bos taurus beta-lactoglobulin. In addition, the proteins present in buffalo cheese whey were hydrolyzed with pepsin and with different combinations of trypsin, chymotrypsin and carboxypeptidase-A. When the TNBS method was used the obtained hydrolysates presented DH of 55 and 62% for H1 and H2, respectively. Otherwise for the OPA method the DH was 27 and 43% for H1 and H2, respectively. The total antioxidant activities of the H1 and H2 samples with and without previous enzymatic hydrolysis, determined by DPPH using diphenyl-p-picrylhydrazyl radical, was 4.9 and 12 mM of Trolox equivalents (TE) for H2 and H2Dint, respectively. The increased concentrations for H1 and H2 samples were approximately 99% and 75%, respectively. The in vitro gastrointestinal digestion efficiency for the samples that were first hydrolyzed was higher compared with samples not submitted to previous hydrolysis. After in vitro gastrointestinal digestion, several amino acids were released in higher concentrations, and most of which were essential amino acids. These results suggest that buffalo cheese whey is a better source of bioavailable amino acids than bovine cheese whey.
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP Univ EstadualPaulista, Departamento de Alimentos e Nutrição, Araraquara - SP, Brazil.
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Bassan
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP Univ EstadualPaulista, Departamento de Alimentos e Nutrição, Araraquara - SP, Brazil.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Goulart
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP Univ EstadualPaulista, Departamento de Alimentos e Nutrição, Araraquara - SP, Brazil.
authors.forename: Ana L M
authors.initials: AL
authors.lastname: Nasser
authors.affiliation: Instituto de Química, UNESP Univ EstadualPaulista, Departamento de Bioquímica e Química Tecnológica, Araraquara - SP, Brazil.
authors.forename: Thaís M S
authors.initials: TM
authors.lastname: Bezerra
authors.affiliation: Instituto de Química, UNESP Univ EstadualPaulista, Departamento de Bioquímica e Química Tecnológica, Araraquara - SP, Brazil.
authors.forename: Saulo S
authors.initials: SS
authors.lastname: Garrido
authors.affiliation: Universidade de São Paulo, Departamento de Biologia, Ribeirão Preto - SP, Brazil.
authors.forename: Cynthia B
authors.initials: CB
authors.lastname: Rustiguel
authors.affiliation: Universidade de São Paulo, Departamento de Biologia, Ribeirão Preto - SP, Brazil.
authors.forename: Luis H S
authors.initials: LH
authors.lastname: Guimarães
authors.affiliation: Faculdade de Ciências Farmacêuticas, UNESP Univ EstadualPaulista, Departamento de Alimentos e Nutrição, Araraquara - SP, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Monti
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002020:Buffaloes; D043422:Carboxypeptidases A / Q000737:chemistry; D002417:Cattle; D002611:Cheese / Q000032:analysis*; D002839:Chromans / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D041981:Gastrointestinal Tract / Q000378:metabolism; D006868:Hydrolysis; D007782:Lactoglobulins / Q000737:chemistry; D007785:Lactose / Q000737:chemistry; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry; D000067796:Whey / Q000378:metabolism*; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D007782:Lactoglobulins; D010455:Peptides; D010851:Picrates; D011492:Protein Hydrolysates; D000067816:Whey Proteins; C004931:1,1-diphenyl-2-picrylhydrazyl; D043422:Carboxypeptidases A; D002918:Chymotrypsin; D014357:Trypsin; D007785:Lactose; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid