title: Molecular identification of indigenous manganese solubilising bacterial biodiversity from manganese mining deposits.
abstract: Manganese (Mn) ranks twelfth among the most exuberant metal present in the earth's crust and finds its imperative application in the manufacturing steel, chemical, tannery, glass, and battery industries. Solubilisation of Mn can be performed by several bacterial strains which are useful in developing environmental friendly solutions for mining activities. The present investigation aims to isolate and characterize Mn solubilising bacteria from low grade ores from Sanindipur Manganese mine of Sundargh district in Odisha state of India. Four morphologically distinct bacterial strains showing visible growth on Mn supplemented plates were isolated. Mn solubilising ability of the bacterial strains was assessed by visualizing the lightening of the medium appearing around the growing colonies. Three isolates were gram negative and rod shaped while the remaining one was gram positive, coccobacilli. Molecular identification of the isolates was carried out by 16S rRNA sequencing and the bacterial isolates were taxonomically classified as Bacillus anthrasis MSB 2, Acinetobacter sp. MSB 5, Lysinibacillus sp. MSB 11, and Bacillus sp. MMR-1 using BLAST algorithm. The sequences were deposited in NCBI GenBank with the accession number KP635223, KP635224, KP635225 and JQ936966, respectively. Manganese solubilisation efficiency of 40, 96, 97.5 and 48.5% were achieved by MMR-1, MSB 2, MSB 5 and MSB 11 respectively. The efficiency of Mn solubilisation is suggested with the help of a pH variation study. The results are discussed in relation to the possible mechanisms involved in Manganese solubilisation efficiency of bacterial isolates.
authors.affiliation: Bioengineering & Biomineral Processing Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri Square, Bhubaneswar, India.
authors.forename: Shreya
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Bioengineering & Biomineral Processing Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri Square, Bhubaneswar, India.
authors.forename: Sansuta
authors.initials: S
authors.lastname: Mohanty
authors.affiliation: Bioengineering & Biomineral Processing Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri Square, Bhubaneswar, India.
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Nayak
authors.affiliation: Institute for Applied Environmental Biotechnology, Bhubaneswar, Odisha, India.
authors.forename: Lala B
authors.initials: LB
authors.lastname: Sukla
authors.affiliation: Bioengineering & Biomineral Processing Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri Square, Bhubaneswar, India.
authors.forename: Alok P
authors.initials: AP
authors.lastname: Das
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001406:Bacillaceae / Q000235:genetics; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007194:India; D008345:Manganese / Q000737:chemistry* / Q000378:metabolism*; D008906:Mining; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008345:Manganese
keywords: Bacteria; Isolation; Manganese; Molecular Identification; Solubilisation