title: Ultrasensitive immunoassay for CA125 detection using acid site compound as signal and enhancer.
abstract: A novel sensitive electrochemical immunosensor was proposed for detection of CA125 based on ferrocenecarboxylic acid (FA), HCl-doped polyaniline (H-PANI) and chitosan hydrochloride (CS-HCl), which were used as substrate materials to generate signal and to enlarge specific surface area. The composite of these three materials (acid center compound, FA@H-PANI@CS-HCl) can be connected by hydrogen (H) in each molecule. Ag@Co3O4 nanosheets were used in the immunosensor to amplify the antibody capacity and to enhance the sensitivity of the immunoassay. This kind of sensor was novel, concise and it is the first time to be used in the detection of CA125. The biosensor exhibited low detection limit (0.25 pg/mL) and wide linear range (0.001-25 ng/mL). The stability, selectivity and reproducibility were acceptable. Serum sample analysis proved that the proposed sensor owned well precision and it might be used in potential clinic testing application in the future.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Fan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: dubin61@gmail.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000097:blood* / Q000276:immunology; D048271:Chitosan / Q000737:chemistry; D003035:Cobalt / Q000737:chemistry; D055664:Electrochemical Techniques; D005296:Ferrous Compounds / Q000737:chemistry; D006801:Humans; D007118:Immunoassay; D008565:Membrane Proteins / Q000097:blood* / Q000276:immunology; D000075163:Metallocenes; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D000906:Antibodies; D018394:CA-125 Antigen; D005296:Ferrous Compounds; C451759:MUC16 protein, human; D008565:Membrane Proteins; D000075163:Metallocenes; D010087:Oxides; C416807:polyaniline; C032949:ferrocenecarboxylic acid; D003035:Cobalt; D012834:Silver; D048271:Chitosan; C060728:cobalt oxide
keywords: Acid center compound; Ag@Co(3)O(4); CA125; Immunosensor