title: Rhizophagus irregularis MUCL 41833 can colonize and improve P uptake of Plantago lanceolata after exposure to ionizing gamma radiation in root organ culture.
abstract: Long-lived radionuclides such as (90)Sr and (137)Cs can be naturally or accidentally deposited in the upper soil layers where they emit β/γ radiation. Previous studies have shown that arbuscular mycorrhizal fungi (AMF) can accumulate and transfer radionuclides from soil to plant, but there have been no studies on the direct impact of ionizing radiation on AMF. In this study, root organ cultures of the AMF Rhizophagus irregularis MUCL 41833 were exposed to 15.37, 30.35, and 113.03 Gy gamma radiation from a (137)Cs source. Exposed spores were subsequently inoculated to Plantago lanceolata seedlings in pots, and root colonization and P uptake evaluated. P. lanceolata seedlings inoculated with non-irradiated AMF spores or with spores irradiated with up to 30.35 Gy gamma radiation had similar levels of root colonization. Spores irradiated with 113.03 Gy gamma radiation failed to colonize P. lanceolata roots. P content of plants inoculated with non-irradiated spores or of plants inoculated with spores irradiated with up to 30.35 Gy gamma radiation was higher than in non-mycorrhizal plants or plants inoculated with spores irradiated with 113.03 Gy gamma radiation. These results demonstrate that spores of R. irregularis MUCL 41833 are tolerant to chronic ionizing radiation at high doses.
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium. dmkothamasi@cemde.du.ac.in.|Laboratory of Soil Biology and Microbial Ecology, Department of Environmental Studies, University of Delhi, Delhi, 110 007, India. dmkothamasi@cemde.du.ac.in.
authors.forename: David
authors.initials: D
authors.lastname: Kothamasi
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Jean
authors.initials: J
authors.lastname: Wannijn
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: May
authors.initials: M
authors.lastname: van Hees
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Robin
authors.initials: R
authors.lastname: Nauts
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Axel
authors.initials: A
authors.lastname: van Gompel
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vanhoudt
authors.affiliation: Earth and Life Institute, Applied Microbiology, Mycology, Mycothèque de l'Université catholique de Louvain (MUCL), Université catholique de Louvain, Croix du Sud 2, bte L7.05.06, B-1348, Louvain-la-Neuve, Belgium.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cranenbrouck
authors.affiliation: Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain, Croix du Sud 2, bte L7.05.06, B-1348, Louvain-la-Neuve, Belgium.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Declerck
authors.affiliation: Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium.
authors.forename: Hildegarde
authors.initials: H
authors.lastname: Vandenhove
mesh_terms: D001610:Beta Particles; D005720:Gamma Rays; D055137:Glomeromycota / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D038821:Mycorrhizae / Q000528:radiation effects; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D010943:Plantago / Q000378:metabolism* / Q000382:microbiology; D011839:Radiation, Ionizing; D036226:Seedlings / Q000382:microbiology; D012987:Soil; D013172:Spores, Fungal / Q000378:metabolism / Q000528:radiation effects; D013559:Symbiosis
chemical_list: D012987:Soil; D010758:Phosphorus
keywords: Gamma radiation; Plant P uptake; Plantago lanceolata; Rhizophagus irregularis; Root colonization; Spores