title: Crystallization and preliminary X-ray diffraction studies of frutalin, an α-D-galactose-specific lectin from Artocarpus incisa seeds.
abstract: Frutalin is an α-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and is a powerful tool for tumour biomarker discovery. The crystallization and preliminary X-ray diffraction analysis of this lectin, which was isolated from Artocarpus incisa seeds, are reported here. Frutalin was purified and submitted to mass-spectrometric analysis. Diverse masses at approximately 16 kDa were observed in the deconvoluted spectra, which support the presence of isoforms. The best frutalin crystals were grown within a week in 0.1 M citric acid pH 3.5 which contained 25% PEG 3350 as a precipitant at 293 K, and diffracted to a maximum resolution of 1.81 Å. The monoclinic crystals belonged to space group I2, with unit-cell parameters a = 76.17, b = 74.56, c = 118.98 Å, β = 96.56°. A molecular-replacement solution was obtained which indicated the presence of four monomers per asymmetric unit. Crystallographic refinement of the structure is in progress.
authors.affiliation: Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
authors.forename: Ana Cristina de Oliveira
authors.initials: AC
authors.lastname: Monteiro-Moreira
authors.affiliation: Physics Institute of São Carlos, São Paulo University, Avenida Trabalhador São-carlense 400, Parque Arnold Schimidt, 13566-590 São Carlos-SP, Brazil.
authors.forename: Humberto
authors.initials: H
authors.lastname: D'Muniz Pereira
authors.affiliation: Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
authors.forename: Antonio Eufrasio
authors.initials: AE
authors.lastname: Vieira Neto
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará (UFC), Campus do Pici, Bloco 907, 60451-970 Fortaleza-CE, Brazil.
authors.forename: Frederico Bruno
authors.initials: FB
authors.lastname: Mendes Batista Moreno
authors.affiliation: Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Duarte Pinto Lobo
authors.affiliation: Center of Experimental Biology (Nubex), University of Fortaleza (UNIFOR), 60811-905 Fortaleza-CE, Brazil.
authors.forename: Felipe Domingos
authors.initials: FD
authors.lastname: de Sousa
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará (UFC), Campus do Pici, Bloco 907, 60451-970 Fortaleza-CE, Brazil.
authors.forename: Renato de Azevedo
authors.initials: Rde A
authors.lastname: Moreira
mesh_terms: D031622:Artocarpus / Q000737:chemistry*; D003460:Crystallization; D005690:Galactose / Q000378:metabolism*; D037161:Galectins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry*; D013058:Mass Spectrometry; D012639:Seeds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D037161:Galectins; D037102:Lectins; C570811:frutalin; D005690:Galactose
keywords: Artocarpus incisa; frutalin; jacalin lectin-related family