title: Chitosan grafted low molecular weight polylactic acid for protein encapsulation and burst effect reduction.
abstract: Chitosan and chitosan-grafted polylactic acid as a matrix for BSA encapsulation in a nanoparticle structure were prepared through a polyelectrolyte complexation method with dextran sulfate. Polylactic acid was synthetized via a polycondensation reaction using the non-metal-based initiator methanesulfonic acid and grafted to the chitosan backbone by a coupling reaction, with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide as the condensing agent. The effect of concentration of the polymer matrix utilized herein on particle diameter, ζ-potential, encapsulation efficiency, and the release kinetic of the model protein bovine serum albumin at differing pH levels was investigated. The influence of pH and ionic strength on the behavior of the nanoparticles prepared was also researched. Results showed that grafting polylactic acid to chitosan chains reduced the initial burst effect in the kinetics of BSA release from the structure of the nanoparticles. Furthermore, a rise in encapsulation efficiency of the bovine serum albumin and diminishment in nanoparticle diameter were observed due to chitosan modification. The results suggest that both polymers actually show appreciable encapsulation efficiency; and release rate of BSA. CS-g-PLA is more suitable than unmodified CS as a carrier for controlled protein delivery.
authors.affiliation: Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic.
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Martino
authors.affiliation: Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kucharczyk
authors.affiliation: Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Hlavova 2030/8, Prague 2 128 43, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Zednik
authors.affiliation: Centre of Polymer Systems, University Institute, Tomas Bata University in Zlin, Tr. T. Bati 5678, 760 01 Zlin, Czech Republic. Electronic address: sedlarik@ft.utb.cz.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Sedlarik
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry*; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003692:Delayed-Action Preparations; D011091:Polyesters; D011108:Polymers; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; C033616:poly(lactide); D048271:Chitosan
keywords: 2-Hydroxypropanoic acid (PubChem CID: 612); Bovine serum albumin; Bovine serum albumin (PubChem SID: 56312734); Chitosan; Chitosan (PubChem CID: 71853); Controlled drug delivery; Nanoparticles; Polylactic acid