title: miR-27b overexpression improves mitochondrial function in a Sirt1-dependent manner.
abstract: Resveratrol improves mitochondrial function, and recent evidences demonstrate that miRNAs play important roles in certain effects of resveratrol. In the current study, we found that a microRNA, miR-27b, was significantly induced in a dose-dependent way in skeletal muscle and C2C12 myoblast treated with resveratrol. Our results showed that overexpression of miR-27b could mimic the effects of resveratrol on improving mitochondrial function and glucose uptake in skeletal muscle cells. Subsequently, we found that FOXO1 was a potential target of miR-27b, and the effects of resveratrol on mitochondrial function were significantly affected after inhibition of miR-27b. Moreover, the effects of miR-27b on mitochondrial function were lost after inhibition of Sirt1, although miR-27b and FOXO1 expression were not influenced. Taken together, these data suggested that overexpression of miR-27b could benefit mitochondrial function, while the effects of overexpressed miR-27b were Sirt1-dependent.
authors.affiliation: Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center for Animal & Poultry Science, Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimetal Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture, the Chinese Academy of Sciences, 644 Yuanda Second Road, Changsha, 410125, Hunan Province, People's Republic of China. xhzhou@isa.ac.cn.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory Department, Hunan Guangxiu Hospital, Changsha, 410000, Hunan, China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Zuo
authors.affiliation: Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center for Animal & Poultry Science, Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimetal Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture, the Chinese Academy of Sciences, 644 Yuanda Second Road, Changsha, 410125, Hunan Province, People's Republic of China. xinwu@isa.ac.cn.
authors.forename: Wu
authors.initials: W
authors.lastname: Xin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001786:Blood Glucose; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology*; D034622:RNA Interference; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology; D015430:Weight Gain
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D035683:MicroRNAs; C540674:Mirn27 microRNA, mouse; D013267:Stilbenes; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: Glucose uptake; Mitochondrial function; Resveratrol; Sirt1; mir-27b