title: Characteristic of oxidative metabolism in patients with chronic kidney disease depending on etiological nosology.
abstract: Development of chronic kidney disease (CKD) is accompanied by change in the oxidative metabolism. The aim of the study was to assess the parameters of oxidative stress in erythrocytes and plasma of patients with CKD 1 and 2 stages depending on the etiological nosology. Two groups were formed. 1(st) - patients with chronic glomerulonephritis (CG), CKD 1, 2. 2(nd) - patients with chronic pyelonephritis (CP), CKD 1, 2. The concentration of carbonyl derivatives was determined by the method of Levine R.L. Malondialdehyde (MDA) concentration - by the method of Goncharenko M.S. and Lapytova A.M. Median and quartiles of parameters were calculated. Statistically significant difference of data in the comparison groups were assessed using the Mann-Whitney test. In group of patients with CG there is the tendency to reduce the concentration of carbonyl derivatives in erythrocytes Md=12.861nmol/ml (12.468, 15.177) as compared with patients with CP Md=14.346nmol/ml (14.130, 14.537). Significant differences in this indicator in these groups were not detected, p=0.715. The concentration of MDA in erythrocytes in these groups varied in the opposite way. In patients with CG concentration of MDA in erythrocytes Md=5.513 µmol/ml (4.487, 6.282) was higher than this indicator in patients with CP Md=4.744 µmol/ml (3.974, 5.256), these changes were not significant, p=0.256. The concentrations of carbonyl derivatives and MDA in plasma in the compared groups were almost identical. In patients with CG concentration of carbonyl derivatives in plasma was Md=0.390nmol/ml (0.381, 0.528), the content of MDA Md=0.846 µmol/ml (0.714, 1.005). In patients with CP concentration of carbonyl derivatives in plasma was Md=0.424nmol/ml (0.312, 0.485) (when comparing 2 groups p=0.668), the content of MDA Md=0.820 µmol/ml (0.740, 0.979) (p=0.886). Thus, in patients with CKD 1, 2 depending on the etiological nosology features of oxidative metabolism were not found.
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.. Electronic address: kolesnikova.evgeniya@gmail.com.
authors.forename: Yevgeniya
authors.initials: Y
authors.lastname: Kolesnikova
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Demidchik
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.
authors.forename: Larissa
authors.initials: L
authors.lastname: Muravlyova
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.
authors.forename: Vilen
authors.initials: V
authors.lastname: Molotov-Luchanskiy
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Klyuyev
authors.affiliation: Karaganda State Medical University (Faculty of General Medicine), Department of Biochemistry, Kazakhstan.
authors.forename: Irina
authors.initials: I
authors.lastname: Beinikova