title: Maltose modified poly(propylene imine) dendrimers as potential carriers of nucleoside analog 5'-triphosphates.
abstract: Poly(propylene imine) (PPI) dendrimers contained surface maltose modification are proposed as drug carriers for nucleoside analog (NA) 5'-triphosphates. The aim of this study was to investigate the interactions between PPI dendrimers of 3rd (G3) or 4th (G4) generation and cytidine-5'-triphosphate (CTP) by Isothermal Titration Calorimetry method. CTP was used as a model molecule of pyrimidine nucleoside analog-cytarabine (ara-CTP) commonly used in leukemia treatment. Complexes of PPI dendrimers with NAs may help to overcome severe limitations of NAs associated with their low solubility and stability or resistance in cancer cells. In the present work, we evaluated stoichiometry and a mechanism of forming complexes between dendrimers and the nucleotide. Moreover, we examined the efficiency of complex formation in relation to dendrimer generations, a type of dendrimer modification with maltose residues and a type of solvent. It was observed that PPI dendrimers create complexes with CTP with high efficiency that makes them promising candidates for a drug delivery system.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland. Electronic address: aszulcab@gmail.com.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szulc
authors.affiliation: Department of Food Environmental and Nutritional Sciences, University of Milan, Celoria St. 2, 20133 Milan, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Signorelli
authors.affiliation: Department of Food Environmental and Nutritional Sciences, University of Milan, Celoria St. 2, 20133 Milan, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Schiraldi
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Str. 6, 01069 Dresden, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voit
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert-Maculewicz
authors.affiliation: Department of Food Environmental and Nutritional Sciences, University of Milan, Celoria St. 2, 20133 Milan, Italy.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Fessas
mesh_terms: D002151:Calorimetry / Q000379:methods; D003570:Cytidine Triphosphate / Q000008:administration & dosage*; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D008320:Maltose / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D012995:Solubility
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D011126:Polypropylenes; C443641:poly(propyleneimine); D003570:Cytidine Triphosphate; D008320:Maltose
keywords: Complexation; Cytidine 5′-triphosphate (CTP); Isothermal Titration Calorimetry (ITC); Nucleoside analog (NA); Poly(propylene imine) (PPI) dendrimer