title: Magnetic molecularly imprinted polydopamine nanolayer on multiwalled carbon nanotubes surface for protein capture.
abstract: A novel, facile and low cost process for imprinting protein on the surface of magnetic multiwalled carbon nanotubes (MMWNTs) was developed using human serum albumin (HSA) as the template and dopamine as the functional monomer. The magnetic imprinted polymers were characterized with transmission electron microscope (TEM), scanning electron microscope (SEM), Fourier-transform infrared spectrometry (FT-IR), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA) in detail. The maximum adsorption capacity of the magnetic imprinted polymers toward HSA was 66.23 mg g(-1) and it took 20 min to achieve the adsorption equilibrium. The magnetic imprinted polymers exhibited the specific selective adsorption toward HSA. Coupled with high performance liquid chromatography (HPLC) analysis, the magnetic imprinted polymers were used to solid-phase extract and detect HSA in urine samples successfully with the recoveries of 91.95-97.8%.
authors.affiliation: College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China.
authors.forename: Yuli
authors.initials: Y
authors.lastname: Yin
authors.affiliation: College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yan
authors.affiliation: College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China. Electronic address: zhaohuizhang77@163.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000419:Albuminuria / Q000652:urine; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry
chemical_list: D007211:Indoles; D037742:Nanotubes, Carbon; D011108:Polymers; D012709:Serum Albumin; C568283:polydopamine; D052203:Ferrosoferric Oxide
keywords: Human serum albumin; MWNTs; Magnetic solid phase extraction; Molecularly imprinted; Polydopamine