title: Untangling a Repetitive Amyloid Sequence: Correlating Biofilm-Derived and Segmentally Labeled Curli Fimbriae by Solid-State NMR Spectroscopy.
abstract: Curli are functional bacterial amyloids produced by an intricate biogenesis machinery. Insights into their folding and regulation can advance our understanding of amyloidogenesis. However, gaining detailed structural information of amyloids, and their tendency for structural polymorphisms, remains challenging. Herein we compare high-quality solid-state NMR spectra from biofilm-derived and recombinantly produced curli and provide evidence that they adopt a similar, well-defined β-solenoid arrangement. Curli subunits consist of five sequence repeats, resulting in severe spectral overlap. Using segmental isotope labeling, we obtained the unambiguous sequence-specific resonance assignments and secondary structure of one repeat, and demonstrate that all repeats are most likely structurally equivalent.
authors.affiliation: Laboratory of Macromolecular Interactions, Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Germany).
authors.forename: Tobias
authors.initials: T
authors.lastname: Schubeis
authors.affiliation: Laboratory of Macromolecular Interactions, Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Germany).
authors.forename: Puwei
authors.initials: P
authors.lastname: Yuan
authors.affiliation: Laboratory of Macromolecular Interactions, Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Germany).|Department of Physics, Faculty of Science, Suez University, Suez, 43533 (Egypt).
authors.forename: Mumdooh
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Laboratory of Macromolecular Interactions, Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Germany).|NMR-Supported Structural Biology, Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Strasse 10, 13125 Berlin (Germany).
authors.forename: Madhu
authors.initials: M
authors.lastname: Nagaraj
authors.affiliation: NMR-Supported Structural Biology, Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Strasse 10, 13125 Berlin (Germany).
authors.forename: Barth-Jan
authors.initials: BJ
authors.lastname: van Rossum
authors.affiliation: Laboratory of Macromolecular Interactions, Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig (Germany). christiane.ritter@helmholtz-hzi.de.
authors.forename: Christiane
authors.initials: C
authors.lastname: Ritter
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D018441:Biofilms; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000682:Amyloid
keywords: functional amyloid; intein; isotopic labeling; protein structures; solid-state NMR spectroscopy