title: Ex Vivo and In Vivo Lentivirus-Mediated Transduction of Airway Epithelial Progenitor Cells.
abstract: A key challenge in pulmonary gene therapy for cystic fibrosis is to provide long-term correction of the genetic defect. This may be achievable by targeting airway epithelial stem/progenitor cells with an integrating vector. Here, we evaluated the ability of a lentiviral vector, derived from the simian immunodeficiency virus and pseudotyped with F and HN envelope proteins from Sendai virus, to transduce progenitor basal cells of the mouse nasal airways. We first transduced basal cell-enriched cultures ex vivo and confirmed efficient transduction of cytokeratin-5 positive cells. We next asked whether progenitor cells could be transduced in vivo. We evaluated the transduction efficiency in mice pretreated by intranasal administration of polidocanol to expose the progenitor cell layer. Compared to control mice, polidocanol treated mice demonstrated a significant increase in the number of transduced basal cells at 3 and 14 days post vector administration. At 14 days, the epithelium of treated mice contained clusters (4 to 8 adjacent cells) of well differentiated ciliated, as well as basal cells suggesting a clonal expansion. These results indicate that our lentiviral vector can transduce progenitor basal cells in vivo, although transduction required denudation of the surface epithelium prior to vector administration.
authors.forename: Giulia
authors.initials: G
authors.lastname: Leoni
authors.forename: Marguerite Y
authors.initials: MY
authors.lastname: Wasowicz
authors.forename: Mario
authors.initials: M
authors.lastname: Chan
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Meng
authors.forename: Raymond
authors.initials: R
authors.lastname: Farley
authors.forename: Steven L
authors.initials: SL
authors.lastname: Brody
authors.forename: Makoto
authors.initials: M
authors.lastname: Inoue
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: National Heart and Lung Institute, Imperial College, London, United Kingdom, Emmanuel Kaye Building 1b, Manresa Rd, London, SW3 6LR. UK. e.alton@imperial.ac.uk.
authors.forename: Eric W F W
authors.initials: EW
authors.lastname: Alton
authors.forename: Uta
authors.initials: U
authors.lastname: Griesenbach
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000235:genetics / Q000628:therapy*; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000494:pharmacology; D020545:Respiratory Mucosa / Q000166:cytology*; D029082:Sendai virus / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D013234:Stem Cells / Q000166:cytology*; D014132:Trachea / Q000166:cytology*; D014161:Transduction, Genetic
chemical_list: D000077423:Polidocanol; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols