title: Expression and serological reactivity of hemorrhagic enteritis virus hexon protein.
abstract: The aim of this work was to express the recombinant hexon protein of the hemorrhagic enteritis virus, to establish the diagnostic value of this protein for serological detection of antibodies in turkey serum samples and to assess seroprevalence of the infection in the Czech Republic. The N' terminal part of the hexon protein was expressed in a bacterial expression system and used as an antigen in an ELISA test for the detection of hemorrhagic enteritis virus specific antibodies in turkey sera. Validation of the test was performed by comparison with a commercially available ELISA test. Serological reactivity was assessed on a panel of 126 turkey sera by a newly developed ELISA test. Serum samples were taken from turkey farms with the history of hemorrhagic enteritis virus infection, from farms with animals free of infection, and from turkey farms following vaccination. Both ELISA kits gave identical results (100 %) with the tested sera. ELISA based on the recombinant hexon protein thus proved useful and cheaper for detection of antibodies in turkey flocks infected with the hemorrhagic enteritis virus.
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1-3, 612 42, Brno, The Czech Republic. lobovad@vfu.cz.|CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, The Czech Republic. lobovad@vfu.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Lobová
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř. 1-3, 612 42, Brno, The Czech Republic.|CEITEC-Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, The Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Celer
mesh_terms: D000256:Adenoviridae / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014422:Turkeys
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C018315:hexon capsid protein, Adenovirus