title: In vitro evaluation of the hemostatic effectiveness of cryopreserved platelets.
abstract: BACKGROUND
  Cryopreserved platelet (CPP) concentrates exhibit a variety of morphologic and functional alterations that may affect the action of CPP with accelerated platelet (PLT) response and clotting. The objective of this study was to compare the in vitro hemostatic effect of CPP with fresh whole blood (WB) and standard 5-day PLT concentrates (PCs).
  
  
  METHODS
  WB collected from eight healthy donors was used to prepare fresh WB, PLT-depleted WB (TPN), and PLT-restored TPN using CPP (TPN-CPP) or PC (TPN-PC). Clot properties were evaluated with thromboelastometry (ROTEM); adhesion and aggregate formation under high shear (Impact-R); and PLT adhesion, aggregate formation, fibrin formation, and prothrombin activation under medium shear in a perfusion system.
  
  
  RESULTS
  TPN-CPP had faster clot initiation (ROTEM clot time--TPN-CPP 115 sec, WB 194 sec, TPN-PC 161 sec), and CPP contributes to a strong clot with PLT involvement (maximum clot firmness--TPN-CPP 32 mm, WB 62 mm, TPN-PC 59 mm). The Impact-R PLT-covered area with TPN-CPP was less than those of WB and PCs, but aggregate size was the same as WB. PLT coverage in perfusion studies was observed with TPN-CPP, although generally less than both WB and PC. Fibrin was deposited with CPP-restored samples, but did not exceed the level of WB.
  
  
  CONCLUSIONS
  CPPs present a phenotype supporting a moderate increase in the rate of clot formation, form stable PLT clots, and do not present a hypercoagulable phenotype during in vitro functional tests.
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Cid
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Ginés
authors.initials: G
authors.lastname: Escolar
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Galan
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: López-Vilchez
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Molina
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Maribel
authors.initials: M
authors.lastname: Díaz-Ricart
authors.affiliation: Department of Hemotherapy and Hemostasis, CDB, IDIBAPS, Hospital Clinic University of Barcelona, Barcelona, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Lozano
authors.affiliation: Center for Transfusion Medicine Research, Department of Pathology the Geisel School of Medicine at Dartmouth, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Dumont
mesh_terms: D001777:Blood Coagulation / Q000502:physiology; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000009:adverse effects*; D015925:Cryopreservation; D006487:Hemostasis / Q000502:physiology; D006490:Hemostatics; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D010973:Platelet Adhesiveness / Q000502:physiology; D010974:Platelet Aggregation / Q000502:physiology
chemical_list: D006490:Hemostatics