title: Lipid deposition in liver cells: The influence of short form augmenter of liver regeneration.
abstract: OBJECTIVE
  The short form augmenter of liver regeneration (sfALR) is a novel human hepatotrophic growth factor. The aim of this study was to investigate the potential role of sfALR in NAFLD.
  
  
  METHODS
  The free fatty acids (FFA) induced lipid accumulation in mouse liver parenchymal cells was examined by Oil Red O staining and triglyceride level determination. The cell cycle was determined by flow cytometry and the proliferation was assessed by CCK8. The expression levels of gfer, miR-122, srebp-1c, fas, dgat2, acc1 and Lrp1B were assessed by quantitative real-time PCR. Furthermore, the MAPK pathway was detected by western blot.
  
  
  RESULTS
  The results showed that sfALR could alleviate the lipid accumulation in mice both in vivo and in vitro. sfALR relieved the proliferation inhibition and G2 arrest of mouse liver parenchymal cells induced by FFAs. Free fatty acids affected gfer expression in a time-and dose-dependent way. And sfALR suppressed JNK activation, increased miR-122 level and reduced fatty acid synthesis-related gene expression.
  
  
  CONCLUSIONS
  These findings suggested that sfALR could alleviate the severity of fatty liver in mice.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing, P.R. China; Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing, P.R. China; National Engineering Research Center for Protein Drugs, Beijing, P.R. China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China. Electronic address: happyzhoufx@sina.com.
authors.forename: Fuxiang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Radiation Medicine, Beijing, P.R. China; National Engineering Research Center for Protein Drugs, Beijing, P.R. China. Electronic address: superyjt79@163.com.
authors.forename: Juntao
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D022781:Hepatocytes / Q000378:metabolism*; D050356:Lipid Metabolism; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000502:physiology; D065626:Non-alcoholic Fatty Liver Disease / Q000188:drug therapy / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000378:metabolism*; D012720:Severity of Illness Index
chemical_list: D035683:MicroRNAs; C544354:Mirn122 microRNA, mouse; D050862:Oxidoreductases Acting on Sulfur Group Donors; C551231:GFER protein, mouse