title: Selective Detection of Protein Homologues in Serum Using an OmpG Nanopore.
abstract: Outer membrane protein G is a monomeric β-barrel porin that has seven flexible loops on its extracellular side. Conformational changes of these labile loops induce gating spikes in current recordings that we exploited as the prime sensing element for protein detection. The gating characteristics, open probability, frequency, and current decrease, provide rich information for analyte identification. Here, we show that two antibiotin antibodies each induced a distinct gating pattern, which allowed them to be readily detected and simultaneously discriminated by a single OmpG nanopore in the presence of fetal bovine serum. Our results demonstrate the feasibility of directly profiling proteins in real-world samples with minimal or no sample pretreatment.
authors.affiliation: Molecular and Cellular Biology Program and †Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Monifa A
authors.initials: MA
authors.lastname: Fahie
authors.affiliation: Molecular and Cellular Biology Program and †Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Bib
authors.initials: B
authors.lastname: Yang
authors.affiliation: Molecular and Cellular Biology Program and †Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Martin
authors.initials: M
authors.lastname: Mullis
authors.affiliation: Molecular and Cellular Biology Program and †Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Holden
authors.affiliation: Molecular and Cellular Biology Program and †Department of Chemistry, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D001798:Blood Proteins / Q000032:analysis*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D058608:Nanopores; D018272:Porins / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001798:Blood Proteins; D029968:Escherichia coli Proteins; C114347:OmpG protein, E coli; D018272:Porins