title: Butelase-mediated synthesis of protein thioesters and its application for tandem chemoenzymatic ligation.
abstract: Using a recently discovered peptide ligase, butelase 1, we developed a novel method to access protein thioesters in good yield. We successfully combined it with native chemical ligation and sortase-mediated ligation in tandem for protein C-terminal labeling and dual-terminal labeling to exploit the orthogonality of these three ligation methods.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cao
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.
authors.forename: Giang K T
authors.initials: GK
authors.lastname: Nguyen
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.
authors.forename: James P
authors.initials: JP
authors.lastname: Tam
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551. jptam@ntu.edu.sg cfliu@ntu.edu.sg.
authors.forename: Chuan-Fa
authors.initials: CF
authors.lastname: Liu
mesh_terms: D004952:Esters; D010453:Peptide Synthases / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004952:Esters; D010940:Plant Proteins; D010453:Peptide Synthases