title: Generation of gene-target dogs using CRISPR/Cas9 system.
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Qingjian
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Key Laboratory of Model Animal for Disease Study of Ministry of Education, Model Animal Research Center, Nanjing University, Nanjing 210061, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Yunzhong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ouyang
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Long
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wei
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Jige
authors.initials: J
authors.lastname: Xin
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Bentian
authors.initials: B
authors.lastname: Zhao
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Lai
authors.affiliation: Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Ni
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Zhong
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Hu
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: He
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Quanmei
authors.initials: Q
authors.lastname: Yan
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Nana
authors.initials: N
authors.lastname: Fan
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
authors.forename: Guanguan
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.affiliation: Key Laboratory of Model Animal for Disease Study of Ministry of Education, Model Animal Research Center, Nanjing University, Nanjing 210061, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064113:CRISPR-Cas Systems; D004285:Dogs / Q000235:genetics*; D005091:Exons; D005260:Female; D055786:Gene Knockout Techniques; D006984:Hypertrophy / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009135:Muscular Diseases / Q000235:genetics; D055435:Myostatin / Q000235:genetics*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics
chemical_list: D055435:Myostatin; D000094704:RNA, Guide, CRISPR-Cas Systems