title: ShRNA-mediated silencing of the RFC3 gene suppress ovarian tumor cells proliferation.
abstract: Ovarian carcinoma is one of the most common and lethal malignancies in the world. Replication factor C (RFC) plays an important role in DNA replication, DNA damage repair, and checkpoint control during cell cycle progression in all eukaryotes. Our previous study found that one unit of RFC complex, RFC3, is over-expressed in ovarian tumor tissues. However, its role in the development of ovarian carcinoma remains unclear. Western blot and real-time RT-PCR analysis were used to measure the expression of RFC3 in ovarian cancer cells. Lentivirus-mediated RFC3-specific shRNA was used to knock down RFC3 expression in ovarian cancer cells. Furthermore, the effect of RFC3 on tumor cellular proliferation and growth were examined, respectively. The expression level of RFC3 was remarkably up-regulated in ovarian cancer OVCAR-3 cells. With MTS and cell growth assays, the viability and proliferation of RFC3 knocking-down OVCAR-3 cell line were shown to be effectively restrained. Down-regulation of RFC3 expression arrested the cell cycle of OVCAR-3 cell in the S-phase and induced apoptosis. This study suggests that RFC3 may play an important role in the the process of ovarian carcinoma, and that it may be a potential biological treatment target in the future.
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Shen
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Haijuan
authors.initials: H
authors.lastname: Shi
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Shuzhong
authors.initials: S
authors.lastname: Yao
authors.affiliation: The First Affiliated Hospital, Sun Yat-Sen University Guangzhou, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering; D051818:Replication Protein C / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C497562:RFC3 protein, human; D034741:RNA, Small Interfering; D051818:Replication Protein C
keywords: Ovarian carcinoma; apoptosis; cell cycle arrest; cell growth; cell proliferation; replication factor C (RFC)