title: Over-expression 5-aminolevulinic acid synthase 2 in nonerythroid cell may causes protoporphyrin IX accumulation.
abstract: Protoporphyrin IX (PpIX) has been used as an efficient sensitizer in photodynamic diagnose, photodynamic therapy, and sonodynamic therapy. The level of PpIX is very important for diagnose or therapy effects. 5-aminolevulinic acid synthase 2 (ALAS2) is the key enzyme upstream of PpIX synthesis. To increase PpIXaccumulation, ALAS2 overexppression transgenic mice were generated. Plasmid containing alas2 gene was transfected in colonic carcinoma cell lines. Both in tissues of transgenic mice and in colonic carcinoma cells, the amount of PpIXaccumulation did increased. At the meanwhile, level of heme, which down stream of PpIX had not been changed. Overexpression ALAS2 in nonerythriod cells may become a novel approach to cause PpIX accumulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Zou
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Yamagata University, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Nakajima
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Menghua
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Diao
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: zhoulingyun75@163.com.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: gaoxu6712@163.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
keywords: ALAS2; PpIX accumulation; nonerythriod; overexppression