title: Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction.
abstract: Reducing oxidative stress is crucial to prevent hypoxia-reoxygenation (H/R)-induced lung injury. Resveratrol has excellent antioxidant and anti-inflammatory effects, and this study investigated its role in H/R-induced type II pneumocyte dysfunction. H/R conditions increased expression of inflammatory cytokines including interleukin (IL)-1β (142.3 ± 21.2%, P < 0.05) and IL-6 (301.9 ± 35.1%, P < 0.01) in a type II alveolar epithelial cell line (A549), while the anti-inflammatory cytokine IL-10 (64.6 ± 9.8%, P < 0.05) and surfactant proteins (SPs) decreased. However, resveratrol treatment effectively inhibited these effects. H/R significantly activated an inflammatory transcription factor, nuclear factor (NF)-κB, while resveratrol significantly inhibited H/R-induced NF-κB transcription activities. To the best of our knowledge, this is the first study showing resveratrol-mediated reversal of H/R-induced inflammatory responses and dysfunction of type II pneumocyte cells in vitro. The effects of resveratrol were partially mediated by promoting SP expression and inhibiting inflammation with NF-κB pathway involvement. Therefore, our study provides new insights into mechanisms underlying the action of resveratrol in type II pneumocyte dysfunction.
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.
authors.forename: Po-Len
authors.initials: PL
authors.lastname: Liu
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.|Department of Pediatrics, Department of Internal Medicine, Department of Chest Surgery, Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Medical University Hospital , Kaohsiung 807, Taiwan.
authors.forename: Inn-Wen
authors.initials: IW
authors.lastname: Chong
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Lee
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.|Department of Pediatrics, Department of Internal Medicine, Department of Chest Surgery, Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Medical University Hospital , Kaohsiung 807, Taiwan.
authors.forename: Jong-Rung
authors.initials: JR
authors.lastname: Tsai
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Department of Internal Medicine, Department of Chest Surgery, Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Medical University Hospital , Kaohsiung 807, Taiwan.
authors.forename: Chong-Chao
authors.initials: CC
authors.lastname: Hsieh
authors.affiliation: Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University , Kaohsiung 801, Taiwan.
authors.forename: Hsuan-Fu
authors.initials: HF
authors.lastname: Kuo
authors.affiliation: Department of Intensive Care Medicine, Chi Mei Medical Center , Tainan 736, Taiwan.
authors.forename: Wei-Lun
authors.initials: WL
authors.lastname: Liu
authors.affiliation: Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University , Taichung 404, Taiwan.|Department of Medical Research, China Medical University Hospital , Taichung 404, Taiwan.|Department of Psychology, College of Medical and Health Science, Asia University , Taichung 413, Taiwan.
authors.forename: Yung-Hsiang
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Respiratory Therapy, Department of Fragrance and Cosmetic Science, College of Medicine, Kaohsiung Medical University , Kaohsiung 807, Taiwan.|Department of Pediatrics, Department of Internal Medicine, Department of Chest Surgery, Division of Cardiovascular Surgery, Department of Surgery, Kaohsiung Medical University Hospital , Kaohsiung 807, Taiwan.
authors.forename: Hsiu-Lin
authors.initials: HL
authors.lastname: Chen
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D055370:Lung Injury / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D013267:Stilbenes; D000077185:Resveratrol; D010100:Oxygen
keywords: hypoxia; inflammation; reoxygenation; resveratrol; surfactant