title: Expression of angiogenic factors and luteinizing hormone receptors in the corpus luteum of mares induced to ovulate with deslorelin acetate.
abstract: The effects of deslorelin acetate use in inducing ovulation need to be clarified to improve the results of equine embryo transfer. The mRNA abundance for angiogenic factors and LH receptor (LHR) in corpus luteum (CL) was studied in mares with natural (control group [CG]) and induced ovulation with deslorelin acetate (treatment group [TG]; follicles: ≥ 35 mm). Transrectal ultrasonography was used to verify the ovulation day, and on Days 4, 8, and 12 after ovulation (Day 0), CL samples were obtained through ultrasound-guided biopsy. The messenger RNA expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and LHR genes were analyzed by real-time polymerase chain reaction. A positive correlation was observed between VEGF and LHR (P < 0.00001, r = 0.78), and it was possible to detect higher LHR expression in the TG than in the CG on Day 4 (P < 0.05). Moreover, this expression was higher on Days 4 and 8 than on Day 12 in the TG. Basic fibroblast growth factor was also expressed in luteal tissue on all days for both groups; however, these differences were not significant. In conclusion, deslorelin acetate was effective for the induction of ovulation in mares, resulting in higher expression of LHR, especially on the fourth day after ovulation. In addition, VEGF expression was influenced by induced ovulation, with a lower level on Day 12, which is expected in nonpregnant mares.
authors.affiliation: Mauricio de Nassau University, Recife, Pernambuco, Brazil.
authors.forename: Victor N
authors.initials: VN
authors.lastname: Maia
authors.affiliation: Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: André M
authors.initials: AM
authors.lastname: Batista
authors.affiliation: Mauricio de Nassau University, Recife, Pernambuco, Brazil.
authors.forename: Sylvio
authors.initials: S
authors.lastname: Cunha Neto
authors.affiliation: Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Diogo M F
authors.initials: DM
authors.lastname: Silva
authors.affiliation: Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil.
authors.forename: Manoel
authors.initials: M
authors.lastname: Adrião
authors.affiliation: Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil. Electronic address: aurea.wischral@gmail.com.
authors.forename: Aurea
authors.initials: A
authors.lastname: Wischral
mesh_terms: D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006736:Horses / Q000502:physiology*; D010060:Ovulation / Q000502:physiology; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D012333:RNA, Messenger / Q000032:analysis; D011974:Receptors, LH / Q000235:genetics*; D017329:Triptorelin Pamoate / Q000031:analogs & derivatives* / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011974:Receptors, LH; D042461:Vascular Endothelial Growth Factor A; D017329:Triptorelin Pamoate; D016222:Fibroblast Growth Factor 2; C066438:deslorelin
keywords: Basic fibroblast growth factor; Equine; Ovulation inducer; Progesterone; Vascular endothelial growth factor