title: Overhauser Dynamic Nuclear Polarization Studies on Local Water Dynamics.
abstract: Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (<1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
authors.affiliation: Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, USA.
authors.forename: Ilia
authors.initials: I
authors.lastname: Kaminker
authors.affiliation: Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Barnes
authors.affiliation: Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, USA; Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, California, USA. Electronic address: songi@chem.ucsb.edu.
authors.forename: Songi
authors.initials: S
authors.lastname: Han
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D011506:Proteins; D012997:Solvents; D014867:Water
keywords: Hydration dynamics; Overhauser dynamic nuclear polarization; Protein hydration water measurements; Site-directed hydration dynamics; Site-directed spin labeling