title: Effect of the addition CNTs on performance of CaP/chitosan/coating deposited on magnesium alloy by electrophoretic deposition.
abstract: CaP/chitosan/carbon nanotubes (CNTs) coating on AZ91D magnesium alloy was prepared via electrophoretic deposition (EPD) followed by conversion in a phosphate buffer solution (PBS). The bonding between the layer and the substrate was studied by an automatic scratch instrument. The phase compositions and microstructures of the composite coatings were determined by using X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FTIR), Raman spectroscopy and scanning electron microscope (SEM). The element concentration and gentamicin concentration were respectively determined by inductively coupled plasma optical emission spectrometer (ICP-OES) test and ultraviolet spectrophotometer (UV). The cell counting kit (CCK) assay was used to evaluate the cytotoxicity of samples to SaOS-2 cells. The results showed that a few CNTs with their original tubular morphology could be found in the CaP/chitosan coating and they were beneficial for the crystal growth of phosphate and improvement of the coating bonding when the addition amount of CNTs in 500 ml of electrophoretic solution was from 0.05 g to 0.125 g. The loading amount of gentamicin increased and the releasing speed of gentamicin decreased after CNTs was added into the CaP/chitosan coating for immersion loading and EPD loading. The cell viability of Mg based CaP/chitosan/CNTs was higher than that of Mg based CaP/chitosan from 16 days to 90 days.
authors.affiliation: Department of Neuro Intern, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; Pharmaceutical Research Institute in Heilongjiang Province, Jiamusi University, Jiamusi 154007, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neuro Intern, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: wenzhaohui1968@163.com.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Wen
authors.affiliation: Department of Neuro Intern, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Neuro Intern, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. Electronic address: hydlgz1962@163.com.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Chemistry Engineering and Technology, Harbin Institute of Technology, Harbin 150001, China. Electronic address: changsd@hit.edu.cn.
authors.forename: Changsong
authors.initials: C
authors.lastname: Dai
mesh_terms: D000497:Alloys / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D005839:Gentamicins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008274:Magnesium / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D005839:Gentamicins; D037742:Nanotubes, Carbon; D048271:Chitosan; C020243:calcium phosphate; D008274:Magnesium
keywords: Bioactivity; Bonding ability; Carbon nanotubes; Gentamicin; Magnesium alloy