title: GPR17 expressing NG2-Glia: Oligodendrocyte progenitors serving as a reserve pool after injury.
abstract: In the adult brain NG2-glia continuously generate mature, myelinating oligodendrocytes. To which extent the differentiation process is common to all NG2-glia and whether distinct pools are recruited for repair under physiological and pathological conditions still needs clarification. Here, we aimed at investigating the differentiation potential of adult NG2-glia that specifically express the G-protein coupled receptor 17 (GPR17), a membrane receptor that regulates the differentiation of these cells at postnatal stages. To this aim, we generated the first BAC transgenic GPR17-iCreER(T2) mouse line for fate mapping studies. In these mice, under physiological conditions, GPR17(+) cells--in contrast to GPR17(-) NG2-glia--did not differentiate within 3 months, a peculiarity that was overcome after cerebral damage induced by acute injury or ischemia. After these insults, GPR17(+) NG2-glia rapidly reacted to the damage and underwent maturation, suggesting that they represent a 'reserve pool' of adult progenitors maintained for repair purposes.
authors.affiliation: Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, 80336, Germany.|Helmholtz Zentrum Munich, Institute for Stem Cell Research, Neuherberg, 85764, Germany.
authors.forename: Francesca
authors.initials: F
authors.lastname: Viganò
authors.affiliation: Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, 80336, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Schneider
authors.affiliation: Department of Biomolecular Sciences, University of Urbino, Urbino, 61029, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Cimino
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, 20133, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Bonfanti
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, 20133, Italy.|Centro Cardiologico Monzino, Milan, 20138, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Gelosa
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, 20133, Italy.|Centro Cardiologico Monzino, Milan, 20138, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Sironi
authors.affiliation: Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, 20133, Italy.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Abbracchio
authors.affiliation: Physiological Genomics, Biomedical Center, Ludwig-Maximilians University, Munich, 80336, Germany.|Helmholtz Zentrum Munich, Institute for Stem Cell Research, Neuherberg, 85764, Germany.|SYNERGY, Excellence Cluster of Systemic Neurology, Ludwig-Maximilians-University, Munich, 81377, Germany.
authors.forename: Leda
authors.initials: L
authors.lastname: Dimou
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism*; D001921:Brain / Q000473:pathology / Q000502:physiology / Q000503:physiopathology; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology*; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology*; D049109:Cell Proliferation / Q000502:physiology; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000473:pathology / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology; D009836:Oligodendroglia / Q000473:pathology / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D055757:SOXE Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D011509:Proteoglycans; D043562:Receptors, G-Protein-Coupled; D055757:SOXE Transcription Factors; D000099216:Chondroitin Sulfate Proteoglycan 4; C534979:GPR17 protein, mouse; C527924:Sox10 protein, mouse; C485184:enhanced green fluorescent protein
keywords: differentiation; ischemia; oligodendrocyte progenitors; oligodendrogenesis; repair