title: Technological advances in bovine mastitis diagnosis: an overview.
abstract: Bovine mastitis is an economic burden for dairy farmers and preventive control measures are crucial for the sustainability of any dairy business. The identification of etiological agents is necessary in controlling the disease, reducing risk of chronic infections and targeting antimicrobial therapy. The suitability of a detection method for routine diagnosis depends on several factors, including specificity, sensitivity, cost, time in producing results, and suitability for large-scale sampling of milk. This article focuses on current methodologies for identification of mastitis pathogens and for detection of inflammation, as well as the advantages and disadvantages of different methods. Emerging technologies, such as transcriptome and proteome analyses and nano- and microfabrication of portable devices, offer promising, sensitive methods for advanced detection of mastitis pathogens and biomarkers of inflammation. The demand for alternative, fast, and reliable diagnostic procedures is rising as farms become bigger. Several examples of technological and scientific advances are summarized which have given rise to more sensitive, reliable and faster diagnostic results.
authors.affiliation: Interdisciplinary Centre of Research in Animal Health (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal (Duarte, Bexiga)Institute for Systems and Computer Engineering-Microsystems and Nanotechnology (INESC-MN), Lisbon, Portugal (Duarte, Freitas)International Iberian Nanotechnology Laboratory (INL), Braga, Portugal (Freitas) admargduarte@fmv.ulisboa.pt.
authors.forename: Carla M
authors.initials: CM
authors.lastname: Duarte
authors.affiliation: Interdisciplinary Centre of Research in Animal Health (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal (Duarte, Bexiga)Institute for Systems and Computer Engineering-Microsystems and Nanotechnology (INESC-MN), Lisbon, Portugal (Duarte, Freitas)International Iberian Nanotechnology Laboratory (INL), Braga, Portugal (Freitas).
authors.forename: Paulo P
authors.initials: PP
authors.lastname: Freitas
authors.affiliation: Interdisciplinary Centre of Research in Animal Health (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal (Duarte, Bexiga)Institute for Systems and Computer Engineering-Microsystems and Nanotechnology (INESC-MN), Lisbon, Portugal (Duarte, Freitas)International Iberian Nanotechnology Laboratory (INL), Braga, Portugal (Freitas).
authors.forename: Ricardo
authors.initials: R
authors.lastname: Bexiga
mesh_terms: D000818:Animals; D002417:Cattle; D019937:Diagnostic Techniques and Procedures / Q000295:instrumentation / Q000662:veterinary*; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000276:immunology
keywords: Bovine mastitis; diagnostic methods; technological advances