title: Anti-Inflammatory Activity and Changes in Antioxidant Properties of Leaf and Stem Extracts from Vitex mollis Kunth during In Vitro Digestion.
abstract: Vitex mollis is used in traditional Mexican medicine for the treatment of some ailments. However, there are no studies on what happens to the anti-inflammatory activity or antioxidant properties and total phenolic content of leaves and stem extracts of Vitex mollis during the digestion process; hence, this is the aim of this work. Methanolic, acetonic, and hexanic extracts were obtained from both parts of the plant. Extract yields and anti-inflammatory activity (elastase inhibition) were measured. Additionally, changes in antioxidant activity (DPPH and ABTS) and total phenols content of plant extracts before and after in vitro digestion were determined. The highest elastase inhibition to prevent inflammation was presented by hexanic extracts (leaf = 94.63% and stem = 98.30%). On the other hand, the major extract yield (16.14%), antioxidant properties (ABTS = 98.51% and DPPH = 94.47% of inhibition), and total phenols (33.70 mg GAE/g of dried sample) were showed by leaf methanolic extract. Finally, leaf and stem methanolic extracts presented an antioxidant activity increase of 35.25% and 27.22%, respectively, in comparison to their initial values after in vitro digestion process. All samples showed a decrease in total phenols at the end of the digestion. These results could be the basis to search for new therapeutic agents from Vitex mollis.
authors.affiliation: División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega (CUCI), Universidad de Guadalajara (UdeG), Avenida Universidad 1115, Colonia Lindavista, 47820 Ocotlán, JAL, Mexico.
authors.forename: Juan Alfredo
authors.initials: JA
authors.lastname: Morales-Del-Rio
authors.affiliation: División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega (CUCI), Universidad de Guadalajara (UdeG), Avenida Universidad 1115, Colonia Lindavista, 47820 Ocotlán, JAL, Mexico.
authors.forename: Melesio
authors.initials: M
authors.lastname: Gutiérrez-Lomelí
authors.affiliation: División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega (CUCI), Universidad de Guadalajara (UdeG), Avenida Universidad 1115, Colonia Lindavista, 47820 Ocotlán, JAL, Mexico.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Robles-García
authors.affiliation: Coordinación de Genómica Alimentaria, Universidad de la Ciénega del Estado de Michoacán de Ocampo (UCM), Avenida Universidad 3000, Colonia Lomas de la Universidad, 59103 Sahuayo, MICH, Mexico.
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Aguilar
authors.affiliation: Unidad de Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ) AC, Avenida Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, Mexico.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Lugo-Cervantes
authors.affiliation: División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega (CUCI), Universidad de Guadalajara (UdeG), Avenida Universidad 1115, Colonia Lindavista, 47820 Ocotlán, JAL, Mexico.
authors.forename: Pedro Javier
authors.initials: PJ
authors.lastname: Guerrero-Medina
authors.affiliation: Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora (ITSON), 5 de Febrero 818 Sur, Colonia Centro, 85000 Ciudad Obregón, SON, Mexico.
authors.forename: Saul
authors.initials: S
authors.lastname: Ruiz-Cruz
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Rosales y Niños Héroes S/N, 83000 Hermosillo, SON, Mexico.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Cinco-Moroyoqui
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Rosales y Niños Héroes S/N, 83000 Hermosillo, SON, Mexico.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Wong-Corral
authors.affiliation: División de Desarrollo Biotecnológico, Centro Universitario de la Ciénega (CUCI), Universidad de Guadalajara (UdeG), Avenida Universidad 1115, Colonia Lindavista, 47820 Ocotlán, JAL, Mexico.
authors.forename: Carmen Lizette
authors.initials: CL
authors.lastname: Del-Toro-Sánchez