title: Distribution of IL-28B genotypes in patients with hepatitis C and healthy individuals in Jahrom city.
abstract: OBJECTIVE
  The purpose of this study was to compare the distribution of interleukin (IL)-28B genotypes between Iranian healthy individuals and patients with chronic hepatitis C based on the genotype.
  
  
  BACKGROUND
  Polymorphisms in the region of IL-28B gene have been identified as the strongest genetic pretreatment predictor of sustained virological response (SVR) in hepatitis C infection.
  
  
  METHODS
  In this study, 147 patients with chronic hepatitis C and 80 healthy individuals were included. The IL-28B rs12979860 and rs8099917 polymorphisms were genotyped by PCR-RFLP method and the frequency of IL-28B polymorphisms with respect to HCV genotypes was also determined.
  
  
  RESULTS
  The frequencies of rs12979860 TT, CC and CT genotypes in the chronic hepatitis C patients and healthy individuals were as follows: 10.8% vs. 11.3%, 38.7% vs. 46.2% and 50.3% vs. 42.5%. Also, the frequencies of rs8099917 TT, GG and GT genotypes in the chronic hepatitis C patients was 61.9%, 6.1% and 32% and in controls was 47.5%, 11.2% and 41.3%. The differences in the distribution of rs12979860 genotypes and alleles between HCV genotype 1 and HCV genotype 3a infected patients were statistically significant.
  
  
  CONCLUSIONS
  The rs12979860 C allele is the favorable allele for the spontaneous clearance of HCV. It seems that the impact of IL-28B polymorphism on the spontaneous clearance of HCV genotype 3 is more prominent than HCV genotype 1, which results in the observation of higher rs12979860 C allele frequency in chronic hepatitis C patients with HCV genotype 3 than HCV genotype 1.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Seyed Dawood
authors.initials: SD
authors.lastname: Mousavi Nasab
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Rasoul
authors.initials: R
authors.lastname: Baharlou
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran ; Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Piroozmand
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Toghyani
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Enayatollah
authors.initials: E
authors.lastname: Shadmand
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Fazel
authors.affiliation: Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Kaveh
authors.initials: K
authors.lastname: Sadeghi
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Seyed Mohammad Ali
authors.initials: SM
authors.lastname: Hashemi
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Shokouh
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Abulfazl
authors.initials: A
authors.lastname: Gheshlaghi
authors.affiliation: Proteomics Research Center, and Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Nayeb Ali
authors.initials: NA
authors.lastname: Ahmadi
authors.affiliation: Department of Microbiology, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ahmadi Vasmehjani
keywords: Hepatitis C; IL-28B Genotype; Jahrom; Polymorphism