title: Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats.
abstract: A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO3 H-APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kojima
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).|Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Hideo
authors.initials: H
authors.lastname: Takakura
authors.affiliation: Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).|PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 (Japan).
authors.forename: Mako
authors.initials: M
authors.lastname: Kamiya
authors.affiliation: Department of Organ Fabrication, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582 (Japan).
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).|PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 (Japan).
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.affiliation: Open Innovation Center for Drug Discovery, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan).
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan). uranokun@m.u-tokyo.ac.jp.|Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033 (Japan). uranokun@m.u-tokyo.ac.jp.|AMED CREST (Japan), Agency for Medical Research and Development, 1-7-1 Otemachi, Chiyoda-ku, Tokyo, 100-0004 (Japan). uranokun@m.u-tokyo.ac.jp.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
mesh_terms: D000818:Animals; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000008:administration & dosage / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry; D008163:Luminescent Measurements; D015394:Molecular Structure; D051381:Rats; D055647:Rats, Transgenic; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000378:metabolism*
chemical_list: D049408:Luminescent Agents; D017382:Reactive Oxygen Species; D008156:Luciferases
keywords: electron transfer; imaging agents; luminescence; membrane permeability; reactive oxygen species