title: Ring finger protein 166 potentiates RNA virus-induced interferon-β production via enhancing the ubiquitination of TRAF3 and TRAF6.
abstract: Host cells orchestrate the production of IFN-β upon detecting invading viral pathogens. Here, we report that Ring finger protein 166 (RNF166) potentiates RNA virus-triggered IFN-β production. Overexpression of RNF166 rather than its homologous proteins RNF114, RNF125, and RNF138, enhanced Sendai virus (SeV)-induced activation of the IFN-β promoter. Knockdown of endogenous RNF166, but not other RNFs, inhibited the IFN-β production induced by SeV and encephalomyocarditis virus. RNF166 interacted with TRAF3 and TRAF6. SeV-induced ubiquitination of TRAF3 and TRAF6 was suppressed when endogenous RNF166 rather than RNF114/138 was knocked down. These findings suggest that RNF166 positively regulates RNA virus-triggered IFN-β production by enhancing the ubiquitination of TRAF3 and TRAF6.
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yong-Kang
authors.initials: YK
authors.lastname: Yang
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Yang
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Zhong-He
authors.initials: ZH
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of The Ministry of Education, School of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Dan-Ying
authors.initials: DY
authors.lastname: Chen
mesh_terms: D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004686:Encephalomyocarditis virus / Q000502:physiology; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D029082:Sendai virus / Q000502:physiology; D015398:Signal Transduction; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics* / Q000378:metabolism; D048029:TNF Receptor-Associated Factor 6 / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D054875:Ubiquitination; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D034741:RNA, Small Interfering; D048008:TNF Receptor-Associated Factor 3; D048029:TNF Receptor-Associated Factor 6; C090656:TRAF3 protein, human; D016899:Interferon-beta; C477146:RNF114 protein, human; C551868:RNF125 protein, human; C577594:RNF138 protein, human; C000609855:RNF166 protein, human; D044767:Ubiquitin-Protein Ligases