title: Quantitative bioanalytical validation of fosfomycin in human whole blood with volumetric absorptive microsampling.
abstract: BACKGROUND
  Fosfomycin is an antibiotic of considerable interest for the treatment of infection by multidrug-resistant bacteria. Translating microsampling techniques into clinical PK studies may provide effective dosing information to improve patient outcomes and minimize the potential development of resistance.
  
  
  RESULTS
  Accuracy and precision results were within ±15%; the method was validated across the range of 5-2000 µg/ml of fosfomycin using volumetric absorptive microsampling (VAMS) devices.
  
  
  CONCLUSIONS
  The VAMS techniques provide acceptable validation data as assessed for lower limit of quantification, linearity, intra- and interday precision and accuracy, selectivity and matrix effects. Results from the recovery and stability studies suggest challenges remain for the analysis of fosfomycin in whole blood using VAMS.
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: Suzanne L
authors.initials: SL
authors.lastname: Parker
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Pharmacy, Royal Brisbane & Women's Hospital, Brisbane, Australia.
authors.forename: Jason A
authors.initials: JA
authors.lastname: Roberts
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.|Department of Intensive Care Medicine, Royal Brisbane & Women's Hospital, Brisbane, Australia.|Faculty of Health, Queensland University of Technology, Brisbane, Australia.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Lipman
authors.affiliation: Burns, Trauma & Critical Care Research Centre, The University of Queensland, Brisbane, Australia.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Wallis
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood* / Q000378:metabolism / Q000592:standards; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D059788:Dried Blood Spot Testing; D005578:Fosfomycin / Q000097:blood* / Q000378:metabolism / Q000592:standards; D006207:Half-Life; D006801:Humans; D011786:Quality Control; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D000900:Anti-Bacterial Agents; D005578:Fosfomycin