title: Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths.
abstract: The iC-GPC assay (iCubate, Huntsville, AL) provides a molecular option for the rapid, on-demand analysis of positive blood cultures. A preliminary evaluation of the iC-GPC assay using 203 clinical or seeded specimens demonstrated a sensitivity of 93.8% to 100% and a specificity of 98.0% to 100% for the identification of five Gram-positive bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) and three associated genetic resistance determinants (mecA, vanA, and vanB) in positive blood culture broths.
authors.affiliation: Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA Wisconsin Diagnostic Laboratory, Milwaukee, Wisconsin, USA bbuchan@mcw.edu.
authors.forename: Blake W
authors.identifier: http://orcid.org/0000-0002-4111-048X
authors.initials: BW
authors.lastname: Buchan
authors.affiliation: Wisconsin Diagnostic Laboratory, Milwaukee, Wisconsin, USA.
authors.forename: Garrett C
authors.initials: GC
authors.lastname: Reymann
authors.affiliation: Laboratory Alliance of Central New York, Liverpool, New York, USA Department of Pathology, SUNY Upstate Medical University, Syracuse, New York, USA.
authors.forename: Paul A
authors.identifier: http://orcid.org/0000-0002-5068-1137
authors.initials: PA
authors.lastname: Granato
authors.affiliation: Laboratory Alliance of Central New York, Liverpool, New York, USA.
authors.forename: Brenda R
authors.initials: BR
authors.lastname: Alkins
authors.affiliation: Tricore Reference Laboratory, Albuquerque, New Mexico, USA.
authors.forename: Patricia
authors.initials: P
authors.lastname: Jim
authors.affiliation: Tricore Reference Laboratory, Albuquerque, New Mexico, USA Department of Pathology, University of New Mexico, Albuquerque, New Mexico, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Young
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial; D005798:Genes, Bacterial; D005838:Genotype; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity