title: A new method for antimony speciation in plant biomass and nutrient media using anion exchange cartridge.
abstract: A selective separation method based on anion exchange cartridge was developed to determine antimony (Sb) speciation in biological matrices by graphite furnace atomic absorption spectrophotometry (GFAAS). The selectivity of the cartridge towards antimonite [Sb(III)] and antimonate [Sb(V)] reversed in the presence of deionized (DI) water and 2mM citric acid. While Sb(V) was retained by the cartridge in DI water, Sb(III) was retained in citric acid media. At pH 6, Sb(III) and Sb(V) formed Sb(III)- and Sb(V)-citrate complexes, but the cartridge had higher affinity towards the Sb(III)-citrate complex. Separation of Sb(III) was tested at various concentrations in fresh and spent growth media and plant tissues. Our results showed that cartridge-based Sb speciation was successful in plant tissues, which was confirmed by HPLC-ICP-MS. The cartridge retained Sb(III) and showed 92-104% Sb(V) recovery from arsenic hyperaccumulator Pteris vittata roots treated with Sb(III) and Sb(V). The cartridge procedure is an effective alternative for Sb speciation, offering low cost, reproducible results, and simple Sb analysis using GFAAS.
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Rujira
authors.initials: R
authors.lastname: Tisarum
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Ren
authors.affiliation: Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Dong
authors.affiliation: Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
authors.forename: Jason T
authors.initials: JT
authors.lastname: Lessl
authors.affiliation: State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Jiangsu 210046, China; Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA. Electronic address: lqma@ufl.edu.
authors.forename: Lena Q
authors.initials: LQ
authors.lastname: Ma
mesh_terms: D001151:Arsenic / Q000032:analysis* / Q000737:chemistry*; D018533:Biomass; D019343:Citric Acid / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D032495:Pteris / Q000737:chemistry* / Q000254:growth & development; D013054:Spectrophotometry, Atomic / Q000295:instrumentation*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D019343:Citric Acid; D006108:Graphite; D001151:Arsenic
keywords: Antimonate; Antimonite; Antimony; Solid phase extraction; Speciation