title: Normal human thyroid cells, BCPAP, and TPC-1 thyroid tumor cell lines display different profile in both basal and TNF-α-induced CXCL8 secretion.
abstract: CXCL8 is secreted by both normal human thyrocytes (NHT) and thyroid cancer cell lines. CXCL8 displays several tumor-promoting effects and recent evidences indicate that its concentrations within the tumor microenvironment can impact the clinical course of the malignancy. Aim of this study was to compare the basal secretion of CXCL8 among NHT and thyroid cancer cell lines (TPC-1 and BCPAP), and to assess the specific cell response to TNF-α in terms of CXCL8 secretion. NHT primary cultures, TPC-1 and BCPAP cell lines were cultured with or without TNF-α (0, 0.1, 1, 10, and 100 ng/ml). CXCL8 levels were measured in the cell supernatants after 24 h. In basal condition, significant differences in the mean levels of CXCL8 were observed among the three cell types: NHT (110.5 ± 56.2 pg/ml), TPC1 (467.4 ± 43.2 pg/ml), and BCPAP (1731.8 ± 493.3 pg/ml), (F = 35.06; p < 0.0001). TNF-α significantly and in a dose-response manner induced CXCL8 secretion in NHT (F = 25.53; p < 0.00001), TPC-1 (F = 13.38; p < 0.0001), and BCPAP (F = 9.88; p < 0.001) cells. The magnitude of the TNF-α effect (fold-increase vs. basal level of CXCL8) differed significantly among the three cell types (F = 10.47; p < 0.0001). BCPAP were identified as the cells showing the highest basal secretion of CXCL8 and the less responsive to TNF-α. NHT, TPC-1, and BCPAP display significant differences in the secretion of both basal and TNF-α-induced CXCL8 secretion. These results indicate that the mechanisms regulating the secretion of CXCL8 differ in tumor cells harboring different genetic alterations suggesting that specific strategies aimed at inhibiting CXCL8 secretion will be required.
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Coperchini
authors.affiliation: Allergy and Immunology Unit, Fondazione Salvatore Maugeri I.R.C.C.S., Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Pignatti
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Leporati
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Carbone
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Croce
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Magri
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy. luca.chiovato@fsm.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Chiovato
authors.affiliation: Unit of Internal Medicine and Endocrinology, Laboratory for Endocrine Disruptors Fondazione Salvatore Maugeri I.R.C.C.S., University of Pavia, Via Maugeri 10, 27100, Pavia, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Rotondi
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D000072637:Thyroid Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D013961:Thyroid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha
keywords: BCPAP; CXCL8; Primary cultures; TNF-α; TPC-1; Thyrocytes