title: Carvedilol Suppresses Apoptosis and Ion Channel Remodelling of HL-1 Cardiac Myocytes Expressing E334K cMyBPC.
abstract: BACKGROUND
  Besides its antiarrhythmic action, carvedilol has an activity to suppress cardiac tissue damage. However, it is unknown whether it has any effect on cellular apoptosis and ion channel remodelling.
  
  
  OBJECTIVE
  To know whether carvedilol has any effect on apoptosis and ion channel remodeling of HL-1 cells expressing E334K MyBPC, and comparing it with bisoprolol.
  
  
  METHODS
  We examined effects of carvedilol and bisoprolol on the levels of pro- and anti-apoptotic proteins and ion channels as well as apoptosis of HL-1 cells transfected with E334K MyBPC using Western blot and flow cytometry.
  
  
  RESULTS
  Carvedilol decreased the protein levels of p53, Bax and cytochrome c and increased that of Bcl-2 in HL-1 cells expressing E334K MyBPC. Bisoprolol failed to affect the protein levels. Both carvedilol and bisoprolol increased the protein levels of Cav1.2 but not that of Nav1.5. Carvedilol was stronger than bisoprolol at decreasing the number of annexin-V positive cells in HL-1 cells expressing E334K MyBPC.
  
  
  CONCLUSIONS
  Carvedilol suppressed apoptosis of HL-1 cells expressing E334K MyBPC through modification of pro- and anti-apoptotic proteins, whose was associated with an increase of Cav 1.2 protein expression.
authors.affiliation: Department of Anesthesiology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Endo
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: U
authors.initials: U
authors.lastname: Bahrudin
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Notsu
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tanno
authors.affiliation: Division of Organ Regeneration Surgery, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Onohara
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ikeda
authors.affiliation: Department of Pharmacology, Diponegoro University Faculty of Medicine, Semarang, Indonesia.
authors.forename: B
authors.initials: B
authors.lastname: Surastri
authors.affiliation: Division of Functional Genomics, Research Center for Bioscience and Technology, Tottori University, Yonago, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakayama
authors.affiliation: Department of Biological Regulation, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ninomiya
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shirayoshi
authors.affiliation: Department of Anesthesiology, Tottori University Faculty of Medicine, Yonago, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inagaki
authors.affiliation: Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University, Yonago, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.affiliation: Division of Regenerative Medicine and Therapeutics, Tottori University Graduate School of Medical Science, Yonago, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hisatome
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D017298:Bisoprolol / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism*; D000077261:Carvedilol; D002460:Cell Line; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D011412:Propanolamines / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D000099163:Myosin Binding Protein C
chemical_list: D017298:Bisoprolol; D002227:Carbazoles; D002352:Carrier Proteins; D000077261:Carvedilol; D007473:Ion Channels; D011412:Propanolamines; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D000099163:Myosin Binding Protein C