title: MicroRNA-34a inhibits tumor invasion and metastasis in gastric cancer by targeting Tgif2.
abstract: To study how miR-34a acts as a tumor suppressor in inhibiting the invasion and metastasis of the gastric cancer cells. First, real-time polymerase chain reaction (PCR) and western blot analysis were used to analyze the expression of miR-34a and Tgif2 in gastric cancer tissues and the adjacent normal tissues. Next, gastric cancer cells were transfected with miR-34a mimic and Tgif2 siRNA, respectively. After transfection, real-time PCR and western blot analysis were used to detect the relative Tgif2 expression level. Cell proliferation was monitored by the colorimetric water-soluble tetrazolium salt and apoptosis analysis was performed with Annexin-V-FITC Apoptosis Detection Kit I. The expression of miR-34a in the adjacent non-tumor tissues was higher than that in gastric cancer tissues, but Tgif2 was opposite. In gastric cancer cells transfected with miR-34a mimic/Tgif siRNA, Tgif2 expression was remarkably down-regulated. Cells transfected with miR-34a mimic/Tgif2 siRNA grew more slowly than the control groups. The percentage of apoptotic cells in gastric cancer cells transfected with miR-34a mimic/Tgif siRNA was much higher compared to the controls. Therefore, we concluded that miR-34a could inhibit tumor invasion and metastasis in gastric cancer by targeting Tgif2 and may be a novel therapeutic candidate for gastric cancer.
authors.affiliation: Department of General Surgery, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital Chengdu 610072, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University Chongqing 400016, China.
authors.forename: Qingha
authors.initials: Q
authors.lastname: Pu
authors.affiliation: Department of General Surgery, Wenchuan Country Hospital of Traditional Chinese Medicine Wenchuan 623000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Cui
authors.affiliation: Department of Blood Transfusion, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital Chengdu 610072, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection
chemical_list: D018398:Homeodomain Proteins; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; C414585:TGIF2 protein, human
keywords: Gastric cancer cells; MiR-34a; Tgif2; apoptosis; cell proliferation