title: Acidipila dinghuensis sp. nov., an acidobacterium isolated from forest soil.
abstract: An aerobic, chemoheterotrophic, non-motile, capsule-forming bacterium designated DHOF10T was isolated from a soil sample collected from the forest of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Strain DHOF10T was able to grow at pH 3.5-8.0 (optimum pH 4.0-4.5) and at 10-37 °C (optimum 28-37 °C). NaCl tolerance was up to 1.0 % (w/v). Major fatty acids consisted of iso-C15 : 0, C18 : 1ω9c and C16 : 1ω7c. The quinone was MK-8 and the DNA G+C content was 56.3 mol%. The polar lipids consisted of phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid, two unidentified aminophospholipids and two unidentified polar lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of genus Acidipila of the phylum Acidobacteria, with the highest 16S rRNA gene sequence similarity of 97.3 % to Acidipila rosea AP8T. On the basis of phylogenetic, phenotypic, physiological and chemotaxonomic distinctiveness, strain DHOF10T represents a novel species of the genus Acidipila, for which the name Acidipila dinghuensis sp. nov. is proposed. The type strain is DHOF10T ( = CGMCC 1.13007T = KCTC 42631T).
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Qiu
mesh_terms: D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8