title: Gold conjugate-based liposomes with hybrid cluster bomb structure for liver cancer therapy.
abstract: Hybrid drug delivery system containing both organic and inorganic nanocarriers is expected to achieve its complementary advantages for the aim of improving the performance of antineoplastic drugs in tumor therapy. Here we report the use of liposomes and gold nanoparticles to construct a liposome with a hybrid Cluster Bomb structure and discuss its unique multi-order drug release property for liver tumor treatment. A very simple method is used for the hybrid liposome preparation and involves mixing two solutions containing liposomes loaded with either non-covalent or covalent Paclitaxel (PTX, namely free PTX or PTX-conjugated GNPs, respectively) by different ratio of volume (25:75, 50:50, 25:75, v/v). Various mixed liposomes were tested to determine the optimal conditions for maximum drug delivery. The optimized liposome was then tested using xenograft Heps tumor-bearing mice and showed the best efficacy for chemotherapeutic inhibition of tumor at PTX liposome: PTX-conjugated GNP liposome of 25:75 ratio (v/v). This system allows for simple and easy preparation while providing a more accurate site- and time-release mode for tumor treatment using antitumor drugs.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
authors.forename: Ai-Yun
authors.initials: AY
authors.lastname: Liu
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Royal Military College of Canada, Kingston, Ontario, Canada.
authors.forename: Vishva
authors.initials: V
authors.lastname: Shah
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
authors.forename: Can
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biomedical Functional Materials, School of Sciences, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hong
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China. Electronic address: ayanju@163.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Ding
mesh_terms: D006046:Gold / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008113:Liver Neoplasms / Q000628:therapy*
chemical_list: D008081:Liposomes; D006046:Gold
keywords: Cluster bomb structure; Gold nanoparticles; Liposomes; Multi-order drug release; Reconstruction