title: Malachite green bioremoval by a newly isolated strain Citrobacter sedlakii RI11; enhancement of the treatment by biosurfactant addition.
abstract: Citrobacter sedlackii RI11, isolated from acclimated textile effluent after selective enrichment on synthetic dyes, was assessed for malachite green (MG) biotreatment potency. Results indicate that this bacterium has potential for use in effective treatment of MG contaminated wastewaters under shaking conditions at neutral and alkaline pH value, characteristic of typical textile effluents. Also, the newly isolated strain can tolerate higher doses of dye and decolorize up to 1,000 mg/l of dye. When used as microbial surfactant to enhance MG biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized the decolorization efficiency at an optimal concentration of biosurfactant of about 0.075%. Studies ensured that MG removal by this strain could be due to biodegradation and/or adsorption. Results on germination potencies of different seeds using the treated dyes under different conditions favor the use of SPB1 biosurfactant for the treatment of MG.
authors.affiliation: Unité 'Enzymes et Bioconversion', National School of Engineers of Sfax, Université de Sfax, BP W 3038 Sfax, Tunisia and Higher Institute of Biotechnology of Sfax, Université de Sfax, Tunisia E-mail: inesmnif2011@gmail.com.
authors.forename: Inès
authors.initials: I
authors.lastname: Mnif
authors.affiliation: Unité 'Enzymes et Bioconversion', National School of Engineers of Sfax, Université de Sfax, BP W 3038 Sfax, Tunisia and Higher Institute of Biotechnology of Sfax, Université de Sfax, Tunisia E-mail: inesmnif2011@gmail.com.
authors.forename: Raouia
authors.initials: R
authors.lastname: Fendri
authors.affiliation: Unité 'Enzymes et Bioconversion', National School of Engineers of Sfax, Université de Sfax, BP W 3038 Sfax, Tunisia and Higher Institute of Biotechnology of Sfax, Université de Sfax, Tunisia E-mail: inesmnif2011@gmail.com.
authors.forename: Dhouha
authors.initials: D
authors.lastname: Ghribi
mesh_terms: D000327:Adsorption; D001412:Bacillus subtilis / Q000737:chemistry; D001673:Biodegradation, Environmental; D002954:Citrobacter / Q000302:isolation & purification / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D055666:Lipopeptides / Q000737:chemistry*; D031224:Raphanus; D012394:Rosaniline Dyes / Q000378:metabolism* / Q000633:toxicity; D045868:Sorghum; D013501:Surface-Active Agents / Q000737:chemistry*; D013784:Textiles; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D004396:Coloring Agents; D055666:Lipopeptides; D012394:Rosaniline Dyes; D013501:Surface-Active Agents; C005095:malachite green