title: Red/NIR G-Quadruplex Sensing, Harvesting Blue Light by a Coumarin-Naphthalene Diimide Dyad.
abstract: A conceptually new light-up nucleic acid fluorescent probe resulting from the conjugation of a coumarin to a naphthalene diimide exhibits a single wavelength emission at 498 nm when free in solution and an additional red/NIR emission when bound to G-quadruplex DNA. The light-up response centred at 666 nm is highly specific for quadruplex DNA when compared to duplex DNA or to RNA quadruplexes.
authors.affiliation: Dipartimento di Chimica, Università di Pavia, V.le Taramelli 10, 27100, Pavia (Italy).
authors.forename: Michela
authors.initials: M
authors.lastname: Zuffo
authors.affiliation: Dipartimento di Chimica, Università di Pavia, V.le Taramelli 10, 27100, Pavia (Italy).
authors.forename: Filippo
authors.initials: F
authors.lastname: Doria
authors.affiliation: Dipartimento di Chimica, Università di Pavia, V.le Taramelli 10, 27100, Pavia (Italy).
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Spalluto
authors.affiliation: Department of Bioengineering, Imperial College London, Royal School of Mines building, South Kensington Campus, London SW7 2AZ (UK).
authors.forename: Sylvain
authors.initials: S
authors.lastname: Ladame
authors.affiliation: Dipartimento di Chimica, Università di Pavia, V.le Taramelli 10, 27100, Pavia (Italy). mauro.freccero@unipv.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Freccero
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D007094:Imides / Q000737:chemistry* / Q000378:metabolism; D008027:Light; D009281:Naphthalenes / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D007094:Imides; D009281:Naphthalenes; C542131:naphthalenediimide; D012313:RNA; D004247:DNA; C030123:coumarin
keywords: G-quadruplexes; dyes; fluorescent probes; naphthalene diimide; nucleic acids