title: Nanopore detection of double stranded DNA using a track-etched polycarbonate membrane.
abstract: We investigate the resistive-pulse sensing of 50-bp DNA using track-etched polycarbonate (PC) nanopores and show the translocation dynamics originating from the electrophoretic transport of DNAs. Conically shaped PC nanopore membranes have been prepared with asymmetric chemical etching technique. We show the potential and concentration dependence of DNA translocation through a PC nanopore. We find that the translocation of DNA scales linearly with both potential and concentration. Additionally, the threshold potential is determined to complete the translocation. Finally, by investigating the current-pulse amplitudes of nanopores with different tip sizes, we show that the nanopore size can be successfully used to distinguish the DNA molecules. These results suggest great promise for the sensing of short DNAs and understanding the dynamics of the translocation process using chemically-etched PC nanopores.
authors.affiliation: Istanbul Medeniyet University, Department of Chemistry, Uskudar 34700, Istanbul. Electronic address: kaan.kececi@medeniyet.edu.tr.
authors.forename: Kaan
authors.initials: K
authors.lastname: Kececi
authors.affiliation: Yildiz Technical University, Department of Chemistry, Esenler 34220, Istanbul.
authors.forename: Nevim
authors.initials: N
authors.lastname: San
authors.affiliation: Yildiz Technical University, Department of Chemistry, Esenler 34220, Istanbul.
authors.forename: Dila
authors.initials: D
authors.lastname: Kaya
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D008567:Membranes, Artificial; D058608:Nanopores; D011076:Polycarboxylate Cement / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D005561:Formates; D008567:Membranes, Artificial; D011076:Polycarboxylate Cement; C030544:formic acid; C005506:polycarbonate; D012972:Sodium Hydroxide; D011189:Potassium Chloride; D004247:DNA
keywords: Biosensing; Conically shaped nanopore; DNA; Resistive-pulse; Track-etched polymer membrane