title: Mitochondrial ascorbate-glutathione cycle and proteomic analysis of carbonylated proteins during tomato (Solanum lycopersicum) fruit ripening.
abstract: In non-photosynthetic tissues, mitochondria are the main source of energy and of reactive oxygen species. Accumulation of high levels of these species in the cell causes damage to macromolecules including several proteins and induces changes in different metabolic processes. Fruit ripening has been characterized as an oxidative phenomenon; therefore, control of reactive oxygen species levels by mitochondrial antioxidants plays a crucial role on this process. In this work, ascorbate-glutathione cycle components, hydrogen peroxide levels and the proteomic profile of carbonylated proteins were analyzed in mitochondria isolated from tomato (Solanum lycopersicum) fruit at two ripening stages. A significant increase on most ascorbate-glutathione cycle components and on carbonylated proteins was observed in mitochondria from breaker to light red stage. Enzymes and proteins involved in diverse cellular and mitochondrial metabolic pathways were identified among the carbonylated proteins. These results suggest that protein carbonylation is a post-translational modification involved in tomato fruit ripening regulation.
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico. Electronic address: omarlopezvidal@yahoo.com.mx.
authors.forename: O
authors.initials: O
authors.lastname: López-Vidal
authors.affiliation: Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Camejo
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Rivera-Cabrera
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Konigsberg
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico.
authors.forename: J M
authors.initials: JM
authors.lastname: Villa-Hernández
authors.affiliation: Cátedra Amelia Sámano Bishop, Universidad Autónoma Metropolitana, Colegio de Ciencias y Humanidades, Plantel Casa Libertad Universidad Autónoma de la Ciudad de México, Mexico.
authors.forename: J A
authors.initials: JA
authors.lastname: Mendoza-Espinoza
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico.
authors.forename: L J
authors.initials: LJ
authors.lastname: Pérez-Flores
authors.affiliation: Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Sevilla
authors.affiliation: Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Campus Universitario de Espinardo, 30100 Murcia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
authors.affiliation: Department of Health Sciences, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, 09340 Iztapalapa, México, D.F., Mexico. Electronic address: fdls@xanum.uam.mx.
authors.forename: F
authors.initials: F
authors.lastname: Díaz de León-Sánchez
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry*; D008928:Mitochondria / Q000737:chemistry*; D050050:Protein Carbonylation; D040901:Proteomics
chemical_list: D005978:Glutathione; D001205:Ascorbic Acid
keywords: ASC–GSH cycle; Carbonylated proteins; Reactive oxygen species (ROS); Ripening; Solanum lycopersicum