title: Quercetin protects HCT116 cells from Dichlorvos-induced   oxidative stress and apoptosis.
abstract: The present study was designed to assess the possible protective effects of Quercetin (QUER), a flavonoid with well-known pharmacological effects, against Dichlorvos (DDVP)-induced toxicity in vitro using HCT116 cells. The cytotoxicity was monitored by cell viability, reactive oxygen species (ROS) generation, anti-oxidant enzyme activities, malondialdehyde (MDA) production, and DNA fragmentation. The apoptosis was assessed through the measurement of the mitochondrial transmembrane potential (ΔΨm) and caspase activation. The results indicated that pretreatment of HCT116 cells with QUER, 2 h prior to DDVP exposure, significantly decreased the DDVP-induced cell death, inhibited the ROS generation, modulated the activities of catalase (CAT) and superoxide dismutase (SOD), and reduced the MDA level. The reductions in mitochondrial membrane potential, DNA fragmentation, and caspase activation were also attenuated by QUER. These findings suggest that dietary QUER can protect HCT116 cells against DDVP-induced oxidative stress and apoptosis.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.|Faculty of Sciences of Bizerte, Carthage University, Tunis, Tunisia.
authors.forename: Intidhar
authors.initials: I
authors.lastname: Ben Salem
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.|Faculty of Sciences of Bizerte, Carthage University, Tunis, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Boussabbeh
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Graiet
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Asma
authors.initials: A
authors.lastname: Rhouma
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia. salwaabid@yahoo.fr.
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Salwa
authors.initials: S
authors.lastname: Abid Essefi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004006:Dichlorvos / Q000633:toxicity*; D004789:Enzyme Activation / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007306:Insecticides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D004006:Dichlorvos; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase; D020169:Caspases