title: Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis).
abstract: An astroglial cell line was established from the brain of half smooth tongue sole (Cynoglossus semilaevis) and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein (GFAP), which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2-Mercaptoethanol (2-Me) and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number (2n=42). The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Tian-Zi
authors.initials: TZ
authors.lastname: Wang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, National Lab for Ocean Science and Technology, 266003, China.
authors.forename: Ai
authors.initials: A
authors.lastname: Sun
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, National Lab for Ocean Science and Technology, 266003, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Zhong-Kai
authors.initials: ZK
authors.lastname: Cui
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.chensl@ysfri.ac.cn.
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Chen
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. shazx@ysfri.ac.cn.
authors.forename: Zhen-Xia
authors.initials: ZX
authors.lastname: Sha
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D001921:Brain / Q000166:cytology*; D002417:Cattle; D018929:Cell Culture Techniques; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D003584:Cytological Techniques / Q000379:methods*; D005412:Flatfishes / Q000235:genetics*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D014162:Transfection
chemical_list: D005904:Glial Fibrillary Acidic Protein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Brain astroglial cel; Cynoglossus semilaevis; Half-smooth tongue sole