title: Degradation of Benzene by Pseudomonas veronii 1YdBTEX2 and 1YB2 Is Catalyzed by Enzymes Encoded in Distinct Catabolism Gene Clusters.
abstract: Pseudomonas veronii 1YdBTEX2, a benzene and toluene degrader, and Pseudomonas veronii 1YB2, a benzene degrader, have previously been shown to be key players in a benzene-contaminated site. These strains harbor unique catabolic pathways for the degradation of benzene comprising a gene cluster encoding an isopropylbenzene dioxygenase where genes encoding downstream enzymes were interrupted by stop codons. Extradiol dioxygenases were recruited from gene clusters comprising genes encoding a 2-hydroxymuconic semialdehyde dehydrogenase necessary for benzene degradation but typically absent from isopropylbenzene dioxygenase-encoding gene clusters. The benzene dihydrodiol dehydrogenase-encoding gene was not clustered with any other aromatic degradation genes, and the encoded protein was only distantly related to dehydrogenases of aromatic degradation pathways. The involvement of the different gene clusters in the degradation pathways was suggested by real-time quantitative reverse transcription PCR.
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Daiana
authors.initials: D
authors.lastname: de Lima-Morales
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Diego
authors.initials: D
authors.lastname: Chaves-Moreno
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Wos-Oxley
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Ruy
authors.initials: R
authors.lastname: Jáuregui
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
authors.forename: Ramiro
authors.initials: R
authors.lastname: Vilchez-Vargas
authors.affiliation: Microbial Interactions and Processes Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany dpi@helmholtz-hzi.de.
authors.forename: Dietmar H
authors.initials: DH
authors.lastname: Pieper
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001554:Benzene / Q000378:metabolism*; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010105:Oxygenases; D049308:Dioxygenases; C050303:isopropylbenzene dioxygenase; C439587:extradiol dioxygenase; D001554:Benzene