title: Multilocus sequence types of invasive pneumococcal isolates from Danish infants (0-90 days) 2003-2013.
abstract: BACKGROUND
  A pneumococcal conjugate vaccine (PCV) has been part of the Danish childhood immunization programme since October 2007. It is administered at the ages of 3, 5 and 12 months and healthy infants younger than 90 days are consequently not vaccinated. Initially the PCV-7 vaccine was used but this was replaced by the PCV-13 in April 2010. Vaccination coverage in Denmark is approximately 90%.The aim of this study was to present multilocus sequence typing (MLST) profiles of Streptococcus pneumoniae isolates from Danish infants (0-90 days) with invasive pneumococcal disease (IPD) in the period 2003-2013.
  
  
  RESULTS
  32 IPD isolates were investigated for their MLST profiles. The identified sequence types (STs) had previously been observed in other European countries. Among the clones were ST 306 (serotype 1), ST 180 (serotype 3) and ST 191 (serotype 7F).
  
  
  CONCLUSIONS
  The ST profile distribution in this study is similar to that observed in other European studies and show a variety of STs. Our data show that the majority of STs found in Denmark is also observed in other European countries, indicating that the IPD isolates were from clones generally circulating in Europe.
authors.affiliation: Neisseria and Streptococcus Reference Laboratory, Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. hcs@ssi.dk.
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Slotved
authors.affiliation: Neisseria and Streptococcus Reference Laboratory, Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. tid@ssi.dk.
authors.forename: Tine
authors.initials: T
authors.lastname: Dalby
authors.affiliation: Neisseria and Streptococcus Reference Laboratory, Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark. hof@ssi.dk.
authors.forename: Steen
authors.initials: S
authors.lastname: Hoffmann
mesh_terms: D002999:Clone Cells; D003718:Denmark / Q000453:epidemiology; D005260:Female; D000069443:Heptavalent Pneumococcal Conjugate Vaccine / Q000008:administration & dosage*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D032541:Mass Vaccination; D058885:Multilocus Sequence Typing; D011008:Pneumococcal Infections / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage*; D012189:Retrospective Studies; D065288:Serogroup; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics* / Q000276:immunology
chemical_list: C538862:13-valent pneumococcal vaccine; D000069443:Heptavalent Pneumococcal Conjugate Vaccine; D022242:Pneumococcal Vaccines