title: Lysosomes serve as a platform for hepatitis A virus particle maturation and nonlytic release.
abstract: Early studies on hepatitis A virus (HAV) in cell culture demonstrated the inclusion of several viral particles in an intracellular lipid-bilayer membrane. However, the origin of these virus-associated membranes and the mechanism for the non-lytic release of HAV into bile are still unknown. Analyzing the association of this virus with cell organelles, we found that newly synthesized HAV particles accumulate in lysosomal organelles and that lysosomal enzymes are involved in the maturation cleavage of the virion. Furthermore, by inhibiting the processes of fusion of lysosomes with the plasma membrane, we found that the nonlytic release of HAV from infected cells occurs via lysosome-related organelles.
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Nicole
authors.initials: N
authors.lastname: Seggewiß
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany.
authors.forename: Dajana
authors.initials: D
authors.lastname: Paulmann
authors.affiliation: Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany. dotzauer@uni-bremen.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dotzauer
mesh_terms: D056945:Hep G2 Cells; D006506:Hepatitis A / Q000201:enzymology* / Q000821:virology*; D030041:Hepatitis A virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008247:Lysosomes / Q000821:virology*; D014771:Virion / Q000235:genetics / Q000502:physiology; D057074:Virus Release