title: Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling.
abstract: On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, "frozen-hydrated" specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (~700,000 µm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.
authors.affiliation: Department of Materials Science and Engineering,Northwestern University,2220 Campus Drive,Evanston,IL 60208,USA.
authors.forename: Irene Y T
authors.initials: IY
authors.lastname: Chang
authors.affiliation: Department of Materials Science and Engineering,Northwestern University,2220 Campus Drive,Evanston,IL 60208,USA.
authors.forename: Derk
authors.initials: D
authors.lastname: Joester
keywords: cryo-planning; cryoSEM; freeze fracture; high pressure freezing; ultrastructure