title: Cyanobacterial composition and spatial distribution based on pyrosequencing data in the Gurbantunggut Desert, Northwestern China.
abstract: Cyanobacteria are the primary colonizers and form a dominant component of soil photosynthetic communities in biological soil crusts. They are crucial in improving soil environments, namely accumulating soil carbon and nitrogen. Many classical studies have examined cyanobacterial diversity in desert crusts, but relatively few comprehensive molecular surveys have been conducted. We used 454 pyrosequencing of 16S rRNA to investigate cyanobacterial composition and distribution on regional scales in the Gurbantunggut Desert. The relationship between cyanobacterial distribution and environmental factors was also explored. A total of 24,973 cyanobacteria partial 16S rRNA gene sequences were obtained, and 507OTUs were selected, as most OTUs had very few reads. Among these, 347 OTU sequences were of cyanobacteria origin, belonging to Oscillatoriales, Nostocales, Chroococcales, and uncultured cyanobacterium clone, respectively. Microcoleus vaginatus, Chroococcidiopsis spp. and M. steenstrupii were the dominant species in most areas of the Gurbantunggut Desert. Compared with other desert, the Gurbantunggut Desert differed in the prominence of Chroococcidiopsis spp. and lack of Pseudanabaenales. Species composition and abundance of cyanobacteria also showed distinct variations. Soil texture, precipitation, and nutrients and salt levels affected cyanobacterial distribution. Increased precipitation was helpful in improving cyanobacterial diversity. A higher content of coarse sand promoted the colonization and growth of Oscillatoriales and some phylotypes of Chroococcales. The fine-textured soil with higher nutrients and salts supported more varied populations of cyanobacteria, namely some heterocystous cyanobacteria. The results suggested that the Gurbantunggut Desert was rich in cyanobacteria and that precipitation was a primary regulating factor for cyanobacterial composition on a regional scale.
authors.affiliation: Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, CAS, Urumqi, Xinjiang, China.
authors.forename: Bingchang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Hydrobiology, CAS, Wuhan, Hubei, China.
authors.forename: Renhui
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Hydrobiology, CAS, Wuhan, Hubei, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, CAS, Urumqi, Xinjiang, China.
authors.forename: Yangui
authors.initials: Y
authors.lastname: Su
authors.affiliation: Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, CAS, Urumqi, Xinjiang, China.
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D044822:Biodiversity; D002681:China; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D003889:Desert Climate; D017753:Ecosystem; D004783:Environmental Microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil
keywords: Cyanobacteria; Environmental properties; Gurbantunggut Desert; Pyrosequencing; Species composition