title: Three-Dimensional Superlocalization Imaging of Gliding Mycoplasma mobile by Extraordinary Light Transmission through Arrayed Nanoholes.
abstract: In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding Mycoplasma mobile (M. mobile). The results analyzed with multiple M. mobile bacteria show individual characters and reveal that M. mobile undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision.
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Wonju
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Physics, Gakushuin University , Tokyo 171-8588, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kinosita
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Oh
authors.affiliation: Department of Physics, Gakushuin University , Tokyo 171-8588, Japan.
authors.forename: Nagisa
authors.initials: N
authors.lastname: Mikami
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Heejin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Biology, Graduate School of Science, Osaka City University , Osaka 558-8585, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyata
authors.affiliation: Department of Physics, Gakushuin University , Tokyo 171-8588, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nishizaka
authors.affiliation: School of Electrical and Electronic Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Kim
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D008027:Light; D009174:Mycoplasma / Q000502:physiology*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure
keywords: Mycoplasma mobile; bacterial motility; extraordinary optical transmission; far-field nanoscopy; molecular tracking; nanoaperture arrays; nanoscale sampling; super-resolution