title: Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
abstract: A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS). The results showed that the composition of RS changed significantly, the lignin and hemicellulose decreased while cellulose increased. The biological treatment with a bacterium Sphingobacterium sp. LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C. The saccharification of rice straw co-treated with bacterium Sphingobacterium sp. LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Si
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China. Electronic address: 61080510@qq.com.
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
authors.forename: Nianlei
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Guangxi Academy of Sciences, Nanning City, Guangxi Zhuang Autonomous Region 530007, China.
authors.forename: Deqiang
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
authors.forename: Yine
authors.initials: Y
authors.lastname: Liu
mesh_terms: D056804:Biofuels; D002241:Carbohydrates; D002482:Cellulose / Q000378:metabolism; D003080:Cold Temperature; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D012972:Sodium Hydroxide; D045249:Sphingobacterium / Q000378:metabolism*; D014508:Urea
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D011134:Polysaccharides; D012972:Sodium Hydroxide; C007916:hemicellulose; D014508:Urea; D002482:Cellulose; D008031:Lignin
keywords: Enzymatic hydrolysis; Lignocellulose pretreatment; NaOH/Urea; Rice straw; Sphingobacterium sp. LD-1