title: Role of AINTEGUMENTA-like gene NtANTL in the regulation of tobacco organ growth.
abstract: The Nicotiana tabacum AINTEGUMENTA-like gene (NtANTL), encoding one of AP2/ERF transcription factors, is a putative ortholog of the AtANT gene from Arabidopsis thaliana. In wild-type tobacco plants, the NtANTL gene was expressed in the actively dividing young flowers, shoot apices, and calluses, while the level of its mRNA increased considerably after treatment with exogenous 6-benzylaminopurine, indoleacetic acid and 24-epibrassinolide. We found a positive correlation among the expression levels of NtANTL, cyclin NtCYCD3;1 and cyclin-dependent kinase NtCDKB1-1 genes, suggesting possible molecular links between AINTEGUMENTA and cell cycle regulators in tobacco plants. However, no correlation was observed between NtANTL, NtCYCD3;1 and NtCDKB1-1 expression levels in response to NaCl and ABA. These observations indicate that the transcription factor NtANTL was not involved in the regulation of the cellular response to salinity nor did it affect the expression of NtCYCD3;1 and NtCDKB1-1 when tobacco plants were exposed to salt stress and ABA. In addition, we generated transgenic tobacco plants with both up-regulated and down-regulated expression of the NtANTL gene. Constitutive expression of the NtANTL gene contributed to an increase in the size of leaves and corolla of transgenic plants. Transgenic plants with reduced expression of the NtANTL gene had smaller leaves, flowers and stems, but showed a compensatory increase in the cell size of leaves and flowers. The results show the significance of the NtANTL gene for the control of organ growth by both cell division and expansion in tobacco plants.
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa 450054, Russia; Bashkir State University, Z. Validi str. 32, 450074 Ufa, Russia. Electronic address: kuluev@bk.ru.
authors.forename: Bulat
authors.initials: B
authors.lastname: Kuluev
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa 450054, Russia. Electronic address: avalbaev@yahoo.com.
authors.forename: Azamat
authors.initials: A
authors.lastname: Avalbaev
authors.affiliation: Bashkir State University, Z. Validi str. 32, 450074 Ufa, Russia.
authors.forename: Elina
authors.initials: E
authors.lastname: Nurgaleeva
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa 450054, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Knyazev
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa 450054, Russia.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Nikonorov
authors.affiliation: Institute of Biochemistry and Genetics, Ufa Research Centre, Russian Academy of Sciences, pr. Oktyabrya 71, Ufa 450054, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Chemeris
mesh_terms: D001593:Benzyl Compounds / Q000494:pharmacology*; D060406:Brassinosteroids / Q000494:pharmacology; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D048429:Cell Size; D015536:Down-Regulation; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified; D011687:Purines / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D001593:Benzyl Compounds; D060406:Brassinosteroids; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011687:Purines; D013260:Steroids, Heterocyclic; D014157:Transcription Factors; D012965:Sodium Chloride; C030737:indoleacetic acid; C480551:benzylaminopurine; C023623:brassinolide
keywords: AINTEGUMENTA-like; Cell number and size; Nicotiana tabacum L; Organ size