title: In vivo evaluation of injectable calcium phosphate cement composed of Zn- and Si-incorporated β-tricalcium phosphate and monocalcium phosphate monohydrate for a critical sized defect of the rabbit femoral condyle.
abstract: Zinc (Zn) enhances bone formation with mineralization and is an essential element of osteoblastic proliferation. Silicon (Si) is important in apatite formation coupled with the promotion of osteogenesis. The primary focus of this work was the assessment of the bone healing capacity of calcium phosphate cements (CPC) composed of Zn- and Si-incorporated β-tri calcium phosphate (TCP) and mono calcium phosphate mono hydrate (MCPM). Zn- and Si-incorporated β-TCP was synthesized through a sol gel process with varying amounts of Zn: (3, 6, or 9% w/w) and 15% w/w Si. Fabricated CPC samples were characterized by scanning electron microscopy, setting time, injectability, compressive strength and initial pH change with time. Compositional analysis and the effects of Zn and Si on cellular interaction were evaluated by energy dispersive X-ray spectroscopy mapping, viability determination and F-actin assay. The data were used to optimize the CPC formulation. The efficacy of bone healing was investigated via implantation into critical sized rabbit femoral condyle defects for 4 and 8 weeks. CPC cement with 6% (w/w) Zn content was the best candidate for faster bone healing (bone to tibial volume ratio in 8 weeks: 22.78% ± 0.02). Significantly faster degradation was also revealed. Bone healing was significantly delayed when CPC cement with 9% (w/w) Zn was used. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 260-271, 2017.
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong Dong, Cheonan, 330-090, South Korea.
authors.forename: Kallyanashis
authors.initials: K
authors.lastname: Paul
authors.affiliation: InoBone Co. Ltd., InoBone Corporate R&D Center, 408 Entrepreneurship Soonchunhyang University, Asan-si, South Korea.
authors.forename: Byung Yeol
authors.initials: BY
authors.lastname: Lee
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong Dong, Cheonan, 330-090, South Korea.
authors.forename: Celine
authors.initials: C
authors.lastname: Abueva
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong Dong, Cheonan, 330-090, South Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
authors.forename: Hwan Jun
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Surgery, College of Medicine, Soonchunhyang University Hospital, Cheonan, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Bae
authors.affiliation: Department of Regenerative Medicine, College of Medicine, Soonchunhyang University, 366-1 Ssangyong Dong, Cheonan, 330-090, South Korea.|Department of Regenerative Medicine, Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, 366-1 Ssangyong Dong, Cheonan, 330-090, South Korea.
authors.forename: Byong Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001843:Bone Cements / Q000737:chemistry / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D051379:Mice; D011817:Rabbits; D012825:Silicon / Q000737:chemistry / Q000494:pharmacology*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D015032:Zinc; D012825:Silicon
keywords: bone regeneration; calcium phosphate cement; silicon; zinc