title: Phenolic profile, antibacterial and cytotoxic properties of second grade date extract from Tunisian cultivars (Phoenix dactylifera L.).
abstract: The present study aimed to investigate the phenolic profile of second grade date extracts and evaluate their antimicrobial and cytotoxic activities with regard to some pathogenic microorganisms. Phenolic content was analyzed by HPLC. Antimicrobial activity was evaluated by the agar disk diffusion method, and in vitro cytotoxic activity was examined by cell proliferation assay. The results revealed that second grade dates presented three benzoic acids, five cinnamic acids and two flavonoids, with the predominance of ρ-coumaric acid (1998.80μg/100g). The antimicrobial activities showed that the date extracts were active against Gram (+) and Gram (-) bacteria, showing marked activity against Escherichia coli with an inhibition zone of 25mm. Cytotoxicity assays showed that the date extracts were able to inhibit the proliferation of HeLa cell lines. The results confirmed that the date extracts were rich in biologically active compounds that are highly valued in the functional food and nutraceutical industries.
authors.affiliation: Université de Sfax, Laboratoire Valorisation, Analyses et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, route de Soukra, 3038 Sfax, Tunisia. Electronic address: kchaouwissal@yahoo.fr.
authors.forename: Wissal
authors.initials: W
authors.lastname: Kchaou
authors.affiliation: Université de Sfax, Laboratoire Valorisation, Analyses et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, route de Soukra, 3038 Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Abbès
authors.affiliation: University of Sfax, Unité de recherche Biotechnologie et pathologies, Institut Supérieur de Biotechnologie de Sfax, Tunisia.
authors.forename: Riadh Ben
authors.initials: RB
authors.lastname: Mansour
authors.affiliation: Université de Liège, Unité de Technologie des Industries Agro-Alimentaires, Gembloux AgroBioTech, passage des Déportés 2, 5030 Gembloux, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Blecker
authors.affiliation: Université de Sfax, Laboratoire Valorisation, Analyses et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, route de Soukra, 3038 Sfax, Tunisia.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Attia
authors.affiliation: Université de Sfax, Laboratoire Valorisation, Analyses et Sécurité des Aliments, Ecole Nationale d'Ingénieurs de Sfax, route de Soukra, 3038 Sfax, Tunisia. Electronic address: besbes.s@voila.fr.
authors.forename: Souhail
authors.initials: S
authors.lastname: Besbes
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D010636:Phenols / Q000737:chemistry*; D065749:Phoeniceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Antimicrobial; Cytotoxicity; Date; HeLa cell lines; Pathogenic microorganisms; Phenolic profile