title: Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.
abstract: G protein-coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization.
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521.
authors.forename: Ilva E
authors.initials: IE
authors.lastname: Cabrera
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Itallia V
authors.initials: IV
authors.lastname: Pacentine
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Andrew
authors.initials: A
authors.lastname: Lim
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Nayeli
authors.initials: N
authors.lastname: Guerrero
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Krystofova
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521.
authors.forename: Liande
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Michkov
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521.
authors.forename: Jacqueline A
authors.initials: JA
authors.lastname: Servin
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Ahrendt
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Carrillo
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Liza M
authors.initials: LM
authors.lastname: Davidson
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Andrew H
authors.initials: AH
authors.lastname: Barsoum
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Jackie
authors.initials: J
authors.lastname: Cao
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Ronald
authors.initials: R
authors.lastname: Castillo
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Wan-Ching
authors.initials: WC
authors.lastname: Chen
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Alex
authors.initials: A
authors.lastname: Dinkchian
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kim
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Sho M
authors.initials: SM
authors.lastname: Kitada
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Taffani H
authors.initials: TH
authors.lastname: Lai
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Ashley
authors.initials: A
authors.lastname: Mach
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Cristin
authors.initials: C
authors.lastname: Malekyan
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Toua R
authors.initials: TR
authors.lastname: Moua
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Carlos Rojas
authors.initials: CR
authors.lastname: Torres
authors.affiliation: College of Natural and Agricultural Sciences, University of California, Riverside, California 92521.
authors.forename: Alaina
authors.initials: A
authors.lastname: Yamamoto
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, California 92521 Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, California 92521 Katherine.Borkovich@ucr.edu.
authors.forename: Katherine A
authors.initials: KA
authors.lastname: Borkovich
mesh_terms: D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D056726:Genetic Association Studies; D005810:Multigene Family; D009154:Mutation; D009492:Neurospora crassa / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D012100:Reproduction, Asexual / Q000235:genetics; D015398:Signal Transduction
chemical_list: D043562:Receptors, G-Protein-Coupled
keywords: G protein−coupled receptors; Heterotrimeric G proteins; filamentous fungi; functional genomics; signal transduction