title: Functional microarray analysis of differentially expressed genes in granulosa cells from women with polycystic ovary syndrome related to MAPK/ERK signaling.
abstract: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age. Although its aetiology and pathogenesis remain unclear, recent studies suggest that the dysfunction of granulosa cells may partly be responsible. This study aimed to use cDNA microarray technology to compare granulosa cell gene expression profiles in women with and without PCOS to identify genes that may be aetiologically implicated in the pathogenesis of PCOS. The study cohort included 12 women undergoing in vitro fertilization, six with PCOS and six without PCOS. Differential gene expression profiles were classified by post-analyses of microarray data, followed by western blot analyses to confirm the microarray data of selected genes. In total, 243 genes were differentially expressed (125 upregulated and 118 downregulated) between the PCOS and non-PCOS granulosa cells. These genes are involved in reproductive system development, amino acid metabolism and cellular development and proliferation. Comparative analysis revealed genes involved in the mitogen-activated protein kinase/extracellular regulated kinase (MAPK/ERK) signaling pathways. Western blot analyses confirmed that mitogen-activated protein kinase kinase kinase 4 and phospho-ERK1/2 were decreased in PCOS granulosa cells. This study identified candidate genes involved in MAPK/ERK signaling pathways that may influence the function of granulosa cells in PCOS.
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University Taipei, Taiwan.|Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Chen-Wei
authors.initials: CW
authors.lastname: Lan
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Mei-Jou
authors.initials: MJ
authors.lastname: Chen
authors.affiliation: Genome and Systems Biology Degree Program, National Taiwan University Taipei, Taiwan.
authors.forename: Kang-Yu
authors.initials: KY
authors.lastname: Tai
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Danny Cw
authors.initials: DC
authors.lastname: Yu
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Yu-Chieh
authors.initials: YC
authors.lastname: Yang
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Pey-Shynan
authors.initials: PS
authors.lastname: Jan
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.
authors.forename: Yu-Shih
authors.initials: YS
authors.lastname: Yang
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University Taipei, Taiwan.|Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.|Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan university Taipei, Taiwan.
authors.forename: Hsin-Fu
authors.initials: HF
authors.lastname: Chen
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University Taipei, Taiwan.|Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University Taipei, Taiwan.|Graduate Institute of Medical Genomics and Proteomics, College of Medicine, National Taiwan university Taipei, Taiwan.
authors.forename: Hong-Nerng
authors.initials: HN
authors.lastname: Ho
mesh_terms: D000328:Adult; D015415:Biomarkers; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System; D011085:Polycystic Ovary Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D015415:Biomarkers