title: Protective Effects of Tetramethylpyrazine on Cerebrovascular Regulations in Rats with Chronic Alcoholic Encephalopathy.
abstract: Recent studies showed that pathology of alcoholic encephalopathy was associated with cerebral vascular damage. TMP (tetramethyl- pyrazine) is widely used in the treatment of cerebrovascular diseases, however, it has not been reported whether TMP can relieve alcohol-induced cerebral vascular damages. The study was performed to investigate the learning and memory, cerebrovascular pathological changes and the expressions of vascular endothelial growth factor (VEGF) and serum levelsofendothelin-1 (ET-1) in the rat model of chronic alcoholic encephalopathy, and explore the effects of TMP intervention on alcoholic encephalopathy. In the present study, the rat model of chronic alcoholic encephalopathy was established by the gavage administration of alcohol; the learning and memory ability was tested by Morris water maze; the expression of VEGF was measured by RT-PCR and Western blot; and the serum levels of ET-1 was measured by radioimmunoassay. We found that alcohol intoxication impaired learning and memory, induced VEGF overexpression and increased ET 1 concentrations. TMP intervention improved learning abilities, increased the VEGF expression and reduced ET-1 level. These results indicate that TMP exhibits therapeutic effects on chronic alcoholic encephalopathy.
authors.affiliation: Department of Neurology, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurology, Shandong Qilu Hospital, Jinan 250012, Shandong, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of gynecology and obstetrics, Jinan Central Hospital Affiliated To Shandong University, Jinan 250013, Shandong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Emergency, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Neurology, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Guang Kun
authors.initials: GK
authors.lastname: Feng
authors.affiliation: Department of Neurology, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Yan Ling
authors.initials: YL
authors.lastname: Yin
authors.affiliation: Department of Neurology, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Yan Xia
authors.initials: YX
authors.lastname: Fan
authors.affiliation: Department of Emergency, Jinan Fourth People's Hospital, Jinan 250031, Shandong, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
mesh_terms: D020268:Alcohol-Induced Disorders, Nervous System / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D007858:Learning / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects; D011719:Pyrazines / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis; D014665:Vasodilator Agents / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D019332:Endothelin-1; D011719:Pyrazines; D042461:Vascular Endothelial Growth Factor A; D014665:Vasodilator Agents; C017953:tetramethylpyrazine