title: Preparation and evaluation of an Arg-Gly-Asp-modified chitosan/hydroxyapatite scaffold for application in bone tissue engineering.
abstract: Bone tissue engineering has become a promising method for the repair of bone defects, and the production of a scaffold with high cell affinity and osseointegrative properties is crucial for successful bone substitute. Chitosan (CS)/hydroxyapatite (HA) composite was prepared by in situ compositing combined with lyophilization, and further modified by arginine‑glycine‑aspartic acid (RGD) via physical adsorption. In order to evaluate the cell adhesion rate, viability, morphology, and alkaline phosphatase (ALP) activity, the RGD‑CS/HA scaffold was seeded with bone marrow stromal cells (BMSCs). The osseointegrative properties of the RGD‑CS/HA scaffold were evaluated by in vivo heterotopic ossification and in vivo bone defect repair. After 4 h culture with the RGD‑CS/HA scaffold, the adhesion rate of the BMSCs was 80.7%. After 3 days, BMSCs were fusiform in shape and evenly distributed on the RGD‑CS/HA scaffold. Formation of extracellular matrix and numerous cell‑cell interactions were observed after 48 h of culture, with an ALP content of 0.006 ± 0.0008 U/l/ng. Furthermore, the osseointegrative ability and biomechanical properties of the RGD‑CS/HA scaffold were comparable to that of normal bone tissue. The biocompatibility, cytocompatibility, histocompatibility and osseointegrative properties of the RGD‑CS/HA scaffold support its use in bone tissue engineering applications.
authors.affiliation: Department of Pathogenic Microorganisms, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Baolin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Orthopedics, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Bi
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Orthopedic Surgery, The First Hospital of Harbin City, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Qu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000138:chemical synthesis*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D002448:Cell Adhesion; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry*; D011817:Rabbits; D011859:Radiography; D051381:Rats; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase