title: Haemophilus influenzae isolates survive for up to 20 years at -70 °C in skim milk tryptone glucose glycerol broth (STGGB) if thawing is avoided during re-culture.
abstract: Haemophilus influenzae remains a major cause of disease worldwide requiring continued study. Recently, isolates of Streptococcus pneumoniae and Moraxella catarrhalis, but not H. influenzae, were reported to survive long-term ultra-freeze storage in STGGB. We show that nontypeable H. influenzae isolates survive for up to 20 years when thawing is avoided.
authors.affiliation: Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia. Electronic address: Kim.Hare@menzies.edu.au.
authors.forename: K M
authors.initials: KM
authors.lastname: Hare
authors.affiliation: Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia.
authors.forename: H C
authors.initials: HC
authors.lastname: Smith-Vaughan
authors.affiliation: Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Beissbarth
authors.affiliation: Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia.
authors.forename: A J
authors.initials: AJ
authors.lastname: Leach
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006193:Haemophilus influenzae / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D008892:Milk / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D011309:Preservation, Biological / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
chemical_list: D003470:Culture Media; D010461:Peptones; C001830:tryptones; D005947:Glucose
keywords: Haemophilus influenzae; Isolate survival; Long-term ultra-freeze storage