title: Suppressive Effects of Resveratrol Treatment on The Intrinsic Evoked Excitability of CA1 Pyramidal Neurons.
abstract: OBJECTIVE
  Resveratrol, a phytoalexin, has a wide range of desirable biological actions. Despite a growing body of evidence indicating that resveratrol induces changes in neu- ronal function, little effort, if any, has been made to investigate the cellular effect of res- veratrol treatment on intrinsic neuronal properties.
  
  
  METHODS
  This experimental study was performed to examine the acute effects of resveratrol (100 µM) on the intrinsic evoked responses of rat Cornu Ammonis (CA1) pyramidal neurons in brain slices, using whole cell patch clamp re- cording under current clamp conditions.
  
  
  RESULTS
  Findings showed that resveratrol treatment caused dramatic changes in evoked responses of pyramidal neurons. Its treatment induced a significant (P<0.05) increase in the after hyperpolarization amplitude of the first evoked action potential. Resveratrol-treated cells displayed a significantly broader action potential (AP) when compared with either control or vehicle-treated groups. In addition, the mean instantaneous firing frequency between the first two action potentials was significantly lower in resveratrol-treated neurons. It also caused a significant reduction in the time to maximum decay of AP. The rheobase current and the utilization time were both significantly greater following resveratrol treatment. Neurons exhibited a significantly depolarized voltage threshold when exposed to resveratrol.
  
  
  CONCLUSIONS
  Results provide direct electrophysiological evidence for the inhibitory effects of resveratrol on pyramidal neurons, at least in part, by reducing the evoked neural activity.
authors.affiliation: Neuroscience Research Center and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Neuroscience Research Center, Baqiyatallah (a.s.) University of Medical Sciences, Tehran, Iran.
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Meftahi
authors.affiliation: Department of Biology, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Ghotbedin
authors.affiliation: Shefa Neuroscience Research Center, Khatam Al Anbia Hospital, Tehran, Iran ; Department of Neuroscience, School of Advanced Medical Technology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Eslamizade
authors.affiliation: Neuroscience Research Center and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Hosseinmardi
authors.affiliation: Neuroscience Research Center and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mahyar
authors.initials: M
authors.lastname: Janahmadi
keywords: Action Potential; Electrophysiology; Neurons; Resveratrol; Whole Cell Patch Clamp