title: Harmonizing HeLa cell cytoskeleton behavior by multi-Ti oxide phased nanostructure synthesized through ultrashort pulsed laser.
abstract: Knowledge about cancer cell behavior on heterogeneous nanostructures is relevant for developing a distinct biomaterial that can actuate cancer cells. In this manuscript, we have demonstrated a harmonized approach of forming multi Ti-oxide phases in a nanostructure (MTOP nanostructure) for its unique cancer cell controlling behavior.Conventionally, single phases of TiO2 are used for targeted therapy and as drug carrier systems.In this research, we have shown a biomaterial that can control HeLa cells diligently using a combination of TiO, Ti3O and TiO2 phases when compared to fibroblast (NIH3T3) cells.MTOP-nanostructures are generated by varying the ionization energy in the vapor plume of the ultrashort pulse laser; this interaction with the material allows accurate tuning and composition of phases within the nanostructure. In addition, the lattice spacing of MTOP-nanostructures was analyzed as shown by HR-TEM investigations. An FESEM investigation of MTOP-nanostructures revealed a greater reduction of HeLa cells relative to fibroblast cells. Altered cell adhesion was followed by modulation of HeLa cell architecture with a significant reduction of actin stress fibers.The intricate combination of MTOP-nanostructures renders a biomaterial that can precisely alter HeLa cell but not fibroblast cell behavior, filling a void in the research for a biomaterial to modulate cancer cell behavior.
authors.affiliation: Ultrashort laser nano manufacturing research facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, Canada, M5B 2K3.
authors.forename: Chandramouli
authors.initials: C
authors.lastname: Chinnakkannu Vijayakumar
authors.affiliation: Ultrashort laser nano manufacturing research facility, Department of Mechanical and Industrial Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, Canada, M5B 2K3.
authors.forename: Krishnan
authors.initials: K
authors.lastname: Venkatakrishnan
authors.affiliation: Nano imaging lab, Department of Aerospace Engineering, Ryerson University, 350 Victoria Street, Toronto, ON, Canada, M5B 2K3.
authors.forename: Bo
authors.initials: B
authors.lastname: Tan
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D003599:Cytoskeleton / Q000187:drug effects / Q000528:radiation effects; D004337:Drug Carriers / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D004337:Drug Carriers; C009495:titanium dioxide; D014025:Titanium