title: Neutral community model explains the bacterial community assembly in freshwater lakes.
abstract: Over the past decade, neutral theory has gained attention and recognition for its capacity to explain bacterial community structure (BCS) in addition to deterministic processes. However, no clear consensus has been drawn so far on their relative importance. In a metacommunity analysis, we explored at the regional and local scale the effects of these processes on the bacterial community assembly within the water column of 49 freshwater lakes. The BCS was assessed using terminal restriction fragment length polymorphism (T-RFLP) of the 16S rRNA genes. At the regional scales, results indicated that the neutral community model well predicted the spatial community structure (R(2) mean = 76%) compared with the deterministic factors - which explained only a small fraction of the BCS total variance (less than 14%). This suggests that the bacterial compartment was notably driven by stochastic processes, through loss and gain of taxa. At the local scale, the bacterial community appeared to be spatially structured by stochastic processes (R(2) mean = 65%) and temporally governed by the water temperature, a deterministic factor, even if some bacterial taxa were driven by neutral dynamics. Therefore, at both regional and local scales the neutral community model appeared to be relevant in explaining the bacterial assemblage structure.
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France adelaide.roguet@gmail.com.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Roguet
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Grégory S
authors.initials: GS
authors.lastname: Laigle
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Therial
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Adèle
authors.initials: A
authors.lastname: Bressy
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Soulignac
authors.affiliation: Unité Molécules de Communication et Adaptation des Micro-organismes (UMR 7245), Sorbonne Université, Muséum National d'Histoire Naturelle, Case 39, 57 rue Cuvier, FR 75005 Paris, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Catherine
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (UMR 7618 [UPMC, UPEC, Paris Diderot, CNRS, IRD, INRA]), Université Pierre et Marie Curie, Bâtiment A, 7 quai St Bernard, FR 75005 Paris, France CEREEP-Ecotron Ile De France (UMS 3194 [CNRS, ENS]), Ecole Normale Supérieure, 78 rue du Château, 77140 St-Pierre-lès-Nemours, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Lacroix
authors.affiliation: Écologie Systématique Évolution, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91400, Orsay, France.
authors.forename: Ludwig
authors.initials: L
authors.lastname: Jardillier
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Bonhomme
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (UMR 7618 [UPMC, UPEC, Paris Diderot, CNRS, IRD, INRA]), Université Paris-Est Créteil, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Thomas Z
authors.initials: TZ
authors.lastname: Lerch
authors.affiliation: Laboratoire Eau Environnement et Systèmes Urbains (UMR MA 102), Université Paris-Est, AgroParisTech, Faculté des Sciences et Technologie, 61 avenue du Général de Gaulle, FR 94000 Créteil, France.
authors.forename: Françoise S
authors.initials: FS
authors.lastname: Lucas
mesh_terms: D001419:Bacteria / Q000145:classification*; D058491:Bacterial Load; D004269:DNA, Bacterial / Q000235:genetics; D005602:France; D006863:Hydrogen-Ion Concentration; D060106:Lakes / Q000382:microbiology*; D008954:Models, Biological; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: T-RFLP; bacterioplankton; community structure; deterministic factors; lake; neutral community model