title: Concentration-dependent effects of resveratrol and metabolites on the redox status of human erythrocytes in single-dose studies.
abstract: Dietary trans-resveratrol (RES) is rapidly metabolized into sulfated and glucuronated conjugates in humans. This study focused on the in vitro determination of the antioxidant capacity of RES and its main physiological metabolites and on its relevance in vivo. In vitro, RES, RES-3-O-sulfate (R3S) and 3-O-glucuronide (R3G) showed antioxidant activities at a concentration of 1mM when compared to Trolox using an assay in which the antioxidant inhibits iron-induced linoleic acid oxidation: 0.87±0.08mM Trolox equivalents (TE) for RES, 0.52±0.01mM TE for R3S and 0.36±0.02mM TE for R3G. At a concentration of 1μM, compounds promoted linoleic acid peroxidation (RES -0.30±0.09mM TE, R3S -0.48±0.05mM TE and R3G -0.57±0.07mM TE). To elucidate whether these effects were reflected in vivo, total antioxidant capacity, reactive oxygen species (ROS), conjugated fatty acid dienes (CD), superoxide dismutase (SOD) and catalase (CAT) activities were determined in human plasma and erythrocytes over 24h, after oral intake of either 0.05g RES as piceid or 5g RES. Oral administration of RES did not show an impact on total antioxidant capacity, ROS or CD. However, enzymatic activities of ROS scavenging SOD and CAT were significantly lower after high-dose compared to low-dose administration of RES (P<.03 and P<.01). In conclusion, in healthy subjects, neither 0.05g nor 5g RES changed blood oxidative state, although our in vitro data point to a prooxidative activity of low concentrations of RES and its metabolites, which could be important in vivo for individuals with compromised antioxidant defense capacity.
authors.affiliation: Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. Electronic address: marc.pignitter@univie.ac.at.
authors.forename: Marc
authors.initials: M
authors.lastname: Pignitter
authors.affiliation: Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. Electronic address: katharina.schueller@univie.ac.at.
authors.forename: Katharina
authors.initials: K
authors.lastname: Schueller
authors.affiliation: Deutsche Forschungsanstalt für Lebensmittelchemie, Lise-Meitner-Straße 34, 85354 Freising, Germany. Electronic address: a.burkon@institut-burkon.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Burkon
authors.affiliation: Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria.
authors.forename: Verena
authors.initials: V
authors.lastname: Knorr
authors.affiliation: Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. Electronic address: lauraesefelder@googlemail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Esefelder
authors.affiliation: Department of Clinical Pharmacology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Electronic address: daniel.doberer@meduniwien.ac.at.
authors.forename: Daniel
authors.initials: D
authors.lastname: Doberer
authors.affiliation: Department of Clinical Pharmacology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Electronic address: michael.wolzt@meduniwien.ac.at.
authors.forename: Michael
authors.initials: M
authors.lastname: Wolzt
authors.affiliation: Department of Nutritional and Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. Electronic address: veronika.somoza@univie.ac.at.
authors.forename: Veronika
authors.initials: V
authors.lastname: Somoza
mesh_terms: D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000097:blood; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D017382:Reactive Oxygen Species; D013267:Stilbenes; D000077185:Resveratrol
keywords: Antioxidant capacity; CAT; Human; ROS; Resveratrol; SOD