title: Localized control of oxidized RNA.
abstract: The oxidation of biological molecules by reactive oxygen species (ROS) can render them inactive or toxic. This includes the oxidation of RNA, which appears to underlie the detrimental effects of oxidative stress, aging and certain neurodegenerative diseases. Here, we investigate the management of oxidized RNA in the chloroplast of the green alga Chlamydomonas reinhardtii. Our immunofluorescence microscopy results reveal that oxidized RNA (with 8-hydroxyguanine) is localized in the pyrenoid, a chloroplast microcompartment where CO2 is assimilated by the Calvin cycle enzyme Rubisco. Results of genetic analyses support a requirement for the Rubisco large subunit (RBCL), but not Rubisco, in the management of oxidized RNA. An RBCL pool that can carry out such a 'moonlighting' function is revealed by results of biochemical fractionation experiments. We also show that human (HeLa) cells localize oxidized RNA to cytoplasmic foci that are distinct from stress granules, processing bodies and mitochondria. Our results suggest that the compartmentalization of oxidized RNA management is a general phenomenon and therefore has some fundamental significance.
authors.affiliation: Biology Department & Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke W, Montreal, Quebec, Canada H4B 1R6.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Biology Department & Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke W, Montreal, Quebec, Canada H4B 1R6.
authors.forename: James S
authors.initials: JS
authors.lastname: Dhaliwal
authors.affiliation: Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University, Centre de Recherche le CHU de Quebec, Quebec, Canada G1V 4G2.
authors.forename: Pauline
authors.initials: P
authors.lastname: Adjibade
authors.affiliation: Biology Department & Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke W, Montreal, Quebec, Canada H4B 1R6.
authors.forename: James
authors.initials: J
authors.lastname: Uniacke
authors.affiliation: Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University, Centre de Recherche le CHU de Quebec, Quebec, Canada G1V 4G2.
authors.forename: Rachid
authors.initials: R
authors.lastname: Mazroui
authors.affiliation: Biology Department & Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke W, Montreal, Quebec, Canada H4B 1R6 william.zerges@concordia.ca.
authors.forename: William
authors.initials: W
authors.lastname: Zerges
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D012313:RNA; D012273:Ribulose-Bisphosphate Carboxylase
keywords: Chloroplast; Oxidized RNA; RNA; Rubisco; Stress granule