title: Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs.
abstract: In this study, iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) (PHEMAGA/Fe(3+)) cryogel discs were prepared. The PHEMAGA/Fe(3+) cryogel discs were characterized by elemental analysis, scanning electron microscopy, Fourier transform infrared spectroscopy, swelling tests, and surface area measurements. The PHEMAGA/Fe(3+) cryogel discs had large pores ranging from 10 to 100 µm with a swelling degree of 9.36 g H2O/g cryogel. Effects of pH, temperature, initial catalase concentration, and flow rate on adsorption capacity of the PHEMAGA/Fe(3+) cryogel discs were investigated. Maximum catalase adsorption capacity (62.6 mg/g) was obtained at pH 7.0, 25°C, and 3 mg/ml initial catalase concentration. The PHEMAGA/Fe(3+) cryogel discs were also tested for the purification of catalase from rat liver. After tissue homogenization, purification of catalase was performed using the PHEMAGA/Fe(3+) cryogel discs and catalase was obtained with a yield of 54.34 and 16.67 purification fold.
authors.affiliation: a Biochemistry Division, Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.|b Nanotechnology and Nanomedicine Division , Institute of Science, Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Ilgım
authors.initials: I
authors.lastname: Göktürk
authors.affiliation: c Molecular Biology Division, Department of Biology , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Işık
authors.initials: I
authors.lastname: Perçin
authors.affiliation: a Biochemistry Division, Department of Chemistry , Hacettepe University , Beytepe , Ankara , Turkey.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000818:Animals; D002374:Catalase / Q000737:chemistry / Q000302:isolation & purification*; D059985:Cryogels; D018698:Glutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007502:Iron Chelating Agents / Q000737:chemistry*; D008099:Liver / Q000201:enzymology*; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D051381:Rats; D013696:Temperature
chemical_list: D059985:Cryogels; D007502:Iron Chelating Agents; D008689:Methacrylates; C115674:N-methacryloyl-glutamic acid; D018698:Glutamic Acid; C005044:hydroxyethyl methacrylate; D002374:Catalase
keywords: Catalase; IMAC; cryogel disc; enzyme; glutamic acid; purification