title: The effect of superoxide anion and hydrogen peroxide imbalance on prostate cancer: an integrative in vivo and in vitro analysis.
abstract: The epidemiological impact of SOD2 imbalance on prostate cancer (PC) risk associated with genetic variations has previously been studied. However, we found no previous studies clarifying the nature of SOD2 effects on prostate cancer. Here, we performed integrated in vivo and in vitro protocols that analyzed the association between Ala16Val-SOD2 polymorphism and prostate cancer aggressiveness at the time of diagnosis and evaluated the effect of the imbalance on PC proliferation using the DU-145 PC cell line treated with paraquat and porphyrin. In the pharmacological model, paraquat was used to increase superoxide anion levels and porphyrin was the SOD2 analog. The results confirmed the impact of superoxide-hydrogen peroxide imbalance on PC cell biology since porphyrin decreased cell proliferation and both treatments modulated antioxidant genes. Therefore, our results corroborate previous suggestions that alteration of redox status could be exploited therapeutically in the treatment of PC.
authors.affiliation: Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre, Rua Sarmento Leite, 245, Porto Alegre, RS, 90050-170, Brazil.
authors.forename: Maiquidieli Dal
authors.initials: MD
authors.lastname: Berto
authors.affiliation: Department of Basic Health Sciences, Federal University of Health Sciences of Porto Alegre, Rua Sarmento Leite, 245, Porto Alegre, RS, 90050-170, Brazil. claudia@ufcspa.edu.br.
authors.forename: Claudia Giuliano
authors.initials: CG
authors.lastname: Bica
authors.affiliation: Department of Health Sciences Center, Federal University of Santa Maria, Av Roraima 1000, Prédio 19, Santa Maria, 97105900, Brazil.
authors.forename: Gustavo Pereira
authors.initials: GP
authors.lastname: de Sá
authors.affiliation: Department of Health Sciences Center, Federal University of Santa Maria, Av Roraima 1000, Prédio 19, Santa Maria, 97105900, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Barbisan
authors.affiliation: Department of Health Sciences Center, Federal University of Santa Maria, Av Roraima 1000, Prédio 19, Santa Maria, 97105900, Brazil.
authors.forename: Verônica Farina
authors.initials: VF
authors.lastname: Azzolin
authors.affiliation: Cellular Genetics Laboratory, Department of Biological Sciences, Tokyo Metropolitan University, Minami-Osawa, Hachioji-shi, Tokyo, 192-0397, Japan.
authors.forename: Felipe
authors.initials: F
authors.lastname: Rogalski
authors.affiliation: Department of Health Sciences Center, Federal University of Santa Maria, Av Roraima 1000, Prédio 19, Santa Maria, 97105900, Brazil.
authors.forename: Marta Maria Medeiros Frescura
authors.initials: MM
authors.lastname: Duarte
authors.affiliation: Department of Health Sciences Center, Federal University of Santa Maria, Av Roraima 1000, Prédio 19, Santa Maria, 97105900, Brazil.
authors.forename: Ivana Beatrice Mânica
authors.initials: IB
authors.lastname: da Cruz
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction / Q000187:drug effects; D010269:Paraquat / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000235:genetics; D000099156:Superoxide Dismutase 2
chemical_list: D006861:Hydrogen Peroxide; D010269:Paraquat; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2
keywords: DU-145; Genotypes; Paraquat; Porphyrin; Prostate cancer; Superoxide manganese dependent