title: Enhancing Pharmacokinetics, Tumor Accumulation, and Antitumor Efficacy by Elastin-Like Polypeptide Fusion of Interferon Alpha.
abstract: Genetic fusion of elastin-like polypeptide (ELP) to the C-terminus of interferon alpha (IFN) generates a well-defined IFN-ELP fusion protein with high yield and well-retained bioactivity. The fusion protein significantly enhances pharmacokinetics, tumor accumulation, and antitumor efficacy of interferon alpha in a murine cancer model.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Gao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000493:pharmacokinetics* / Q000627:therapeutic use; D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D016898:Interferon-alpha / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000097:blood / Q000493:pharmacokinetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D016898:Interferon-alpha; D010455:Peptides; D011993:Recombinant Fusion Proteins; D004549:Elastin
keywords: cancer therapy; elastin-like polypeptides; interferon; pharmacokinetics; protein delivery