title: Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains.
abstract: In this study, we isolated an environmental clone of Ochrobactrum intermedium, strain 2745-2, from the formation water of Changqing oilfield in Shanxi, China, which can degrade crude oil. Strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °C and pH 5.5. We sequenced the genome and found a single chromosome of 4 800 175 bp, with a G+C content of 57.63%. Sixty RNAs and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, and metabolizing of crude oil. A comparative genomic analysis with related clinical strains (M86, 229E, and LMG3301(T)) showed that genes involved in virulence, disease, defense, phages, prophages, transposable elements, plasmids, and antibiotic resistance are also present in strain 2745-2.
authors.affiliation: Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing 100083, China.
authors.forename: Lu-jun
authors.initials: LJ
authors.lastname: Chai
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Xia-wei
authors.initials: XW
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing 100083, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Bei-wen
authors.initials: BW
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China.
authors.forename: Fu-chang
authors.initials: FC
authors.lastname: Shu
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China.
authors.forename: Zheng-liang
authors.initials: ZL
authors.lastname: Wang
authors.affiliation: Institute of Porous Flow & Fluid Mechanics, Chinese Academy of Sciences, Langfang 065007, China.
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Cui
authors.affiliation: Institute of Porous Flow & Fluid Mechanics, Chinese Academy of Sciences, Langfang 065007, China.
authors.forename: Han-ping
authors.initials: HP
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 257061, China.
authors.forename: Zhong-zhi
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing 100083, China.
authors.forename: Du-jie
authors.initials: DJ
authors.lastname: Hou
authors.affiliation: College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China.
authors.forename: Yue-hui
authors.initials: YH
authors.lastname: She
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D042041:Ochrobactrum / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010578:Petroleum / Q000382:microbiology*; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D010578:Petroleum
keywords: Comparative genome; Ochrobactrum intermedium; Oil degradation; Pathogen