title: Determination of the structure and morphology of gold nanoparticle-HSA protein complexes.
abstract: We propose a simple method to determine the structure and morphology of nanoparticle protein complexes. By combining a separation method with online size measurements, density measurements and circular dichroism, we could identify the number of proteins bound to each nanoparticle and their secondary structure changes in the complex. This method provides much-needed experimental information on the interaction of proteins with nanoparticles and on the behavior of nanoparticles in biological systems.
authors.affiliation: European Commission, Joint Research Centre, Institute for Health and Consumer Protection, I-21027, Ispra, VA, Italy. luigi.calzolai@jrc.ec.europa.eu.
authors.forename: Robin
authors.initials: R
authors.lastname: Capomaccio
authors.forename: Isaac Ojea
authors.initials: IO
authors.lastname: Jimenez
authors.forename: Pascal
authors.initials: P
authors.lastname: Colpo
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
authors.forename: Giacomo
authors.initials: G
authors.lastname: Ceccone
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.forename: Luigi
authors.initials: L
authors.lastname: Calzolai
mesh_terms: D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D006046:Gold