title: Endogenous and Exogenous Calcium Involved in the Betulin Production from Submerged Culture of Phellinus linteus Induced by Hydrogen Sulfide.
abstract: Using pharmacological and biochemical approaches, Ca(2+) involved in the betulin production in mycelia of Phellinus linteus induced by hydrogen sulfide (H2S) were investigated. The results showed that 2 mM H2S donor NaHS or 10 mM CaCl2 was found to enhance the betulin content in the mycelia of Phellinus to the maximum, which were 112.43 and 93.24% higher than that in the control, respectively. Further, NaHS and CaCl2 co-treatment also showed positive outcome, which were 128.95 or 24.52% higher than that in the control or NaHS treatment. At the same time, NaHS also enhanced the content of Ca(2+) and CaM. But, the above positive inductive effects for Ca(2+), CaM, and betulin production can be blocked with either Ca(2+) channel blocker (LaCl3, 2-aminoethoxydiphenyl borate) or Ca(2+) chelator (ethylenediaminetetraacetic acid (EDTA)). Among of them, betulin content was reduced 35.06% by NaHS and EGTA to the minimum, and this reduction could be reversed by the application of CaCl2 (NaHS + EGTA + CaCl2). From above results, it can be concluded that endogenous and exogenous calcium involved in the betulin production from submerged culture of P. linteus induced by hydrogen sulfide.
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, People's Republic of China. gzf325@126.com.|Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada. gzf325@126.com.
authors.forename: Guizhi
authors.identifier: http://orcid.org/0000-0002-2158-6712
authors.initials: G
authors.lastname: Fan
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Duan
authors.initials: D
authors.lastname: Jian
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D014315:Triterpenes / Q000378:metabolism*; D000094062:Betulinic Acid
chemical_list: D002118:Calcium; D006862:Hydrogen Sulfide; D014315:Triterpenes; C002503:betulin; D000094062:Betulinic Acid
keywords: Betulin; Ca2+; Hydrogen sulfide (H2S); Phellinus linteus; Submerged culture