title: Blood monitoring of perfluorocarbon compounds (F-tert-butylcyclohexane, perfluoromethyldecalin and perfluorodecalin) by headspace-gas chromatography-tandem mass spectrometry.
abstract: A headspace-gas chromatography-tandem mass spectrometry (HS-GC-MS/MS) method for the trace measurement of perfluorocarbon compounds (PFCs) in blood was developed. Due to oxygen carrying capabilities of PFCs, application to doping and sports misuse is speculated. This study was therefore extended to perform validation methods for F-tert-butylcyclohexane (Oxycyte(®)), perfluoro(methyldecalin) (PFMD) and perfluorodecalin (PFD). The limit of detection of these compounds was established and found to be 1.2 µg/mL blood for F-tert-butylcyclohexane, 4.9 µg/mL blood for PFMD and 9.6 µg/mL blood for PFD. The limit of quantification was assumed to be 12 µg/mL blood (F-tert-butylcyclohexane), 48 µg/mL blood (PFMD) and 96 µg/mL blood (PFD). HS-GC-MS/MS technique allows detection from 1000 to 10,000 times lower than the estimated required dose to ensure a biological effect for the investigated PFCs. Thus, this technique could be used to identify a PFC misuse several hours, maybe days, after the injection or the sporting event. Clinical trials with those compounds are still required to evaluate the validation parameters with the calculated estimations.
authors.affiliation: Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Giuliani
authors.affiliation: Swiss Laboratory for Doping Analyses University Centre of Legal Medicine Lausanne - Geneva, CH-1066 Epalinges, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Saugy
authors.affiliation: Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Augsburger
authors.affiliation: Forensic Toxicology and Chemistry Unit University Centre of Legal Medicine Lausanne - Geneva, CH-1011 Lausanne, Switzerland. Electronic address: vincent.varlet@chuv.ch.
authors.forename: V
authors.initials: V
authors.lastname: Varlet
mesh_terms: D003510:Cyclohexanes / Q000097:blood*; D005466:Fluorocarbons / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D003510:Cyclohexanes; D005466:Fluorocarbons; C054362:tert-butylcyclohexane; C008805:perfluorodecalin; C037464:perfluoromethyldecalin
keywords: F-tert-butylcyclohexane; Perfluoro(methyldecalin); Perfluorocarbons; Perfluorodecalin; Tandem mass spectrometry