title: Increased proportions of dendritic cells and recovery of IFNγ responses in HIV/HCV co-infected patients receiving ART.
abstract: Dendritic cell (DC) numbers and functions can be affected by HIV and HCV disease, but the effects of antiretroviral therapy (ART) on DC and the implications of these changes are unclear. We examined circulating DC in samples from Indonesian patients beginning ART with advanced HIV disease and documented mild/moderate HCV hepatitis. Frequencies of myeloid and plasmacytoid DC increased after 6 months on ART, but frequencies of DC producing IL-12 or IFNα following stimulation with TLR agonists (CL075, CpG) did not change. IFNγ responses to CL075, HCV and other antigens rose over this period. Hence increased IFNγ responses during ART may be associated with increased DC frequencies rather than changes in their functional capacity.
authors.affiliation: Research Centre of Virology and Cancer Pathobiology, Faculty of Medicine, University of Indonesia, Indonesia.
authors.forename: Henny
authors.initials: H
authors.lastname: Saraswati
authors.affiliation: School of Biomedical Science, Curtin University of Technology, Bentley, Australia; Department of Microbiology and Infectious Disease, Royal Perth Hospital, Australia. Electronic address: silvia.lee@curtin.edu.au.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lee
authors.affiliation: Stem Cell Unit, Lung Institute of Western Australia/Centre for Asthma, Allergy and Respiratory Research, School of Medicine and Pharmacology, The University of Western Australia, Perth, Australia.
authors.forename: Dino
authors.initials: D
authors.lastname: Tan
authors.affiliation: School of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Indonesia.
authors.forename: Evy
authors.initials: E
authors.lastname: Yunihastuti
authors.affiliation: School of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Indonesia.
authors.forename: Rino
authors.initials: R
authors.lastname: Gani
authors.affiliation: School of Biomedical Science, Curtin University of Technology, Bentley, Australia; School of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital, Indonesia.
authors.forename: Patricia
authors.initials: P
authors.lastname: Price
mesh_terms: D000293:Adolescent; D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D001170:Arthritis, Infectious / Q000276:immunology; D002478:Cells, Cultured; D060085:Coinfection; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D016174:Hepacivirus / Q000276:immunology*; D006526:Hepatitis C / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007214:Indonesia; D008297:Male; D008875:Middle Aged; D011804:Quinolines / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D055815:Young Adult
chemical_list: C551788:CL 075; D016207:Cytokines; D011804:Quinolines; D013844:Thiazoles
keywords: Antiretroviral therapy; Dendritic cells; HCV; HIV