title: MiR-455-3p regulates early chondrogenic differentiation via inhibiting Runx2.
abstract: The expression of miR-455-3p has been shown to be up-regulated in chondrogenesis of mesenchymal stem cell, but its role in different stages during chondrogenesis remains unknown. Here, we show that miR-455-3p is increased in ATDC5 cells from 0 d to 21 d, but rapidly decreases at 28 d, and a similar expression kinetic is detected in the development of mouse embryos. We show that miR-455-3p functions as an activator for early chondrogenic differentiation, most likely by inhibiting the expression of Runt-related transcription factor 2 (Runx2) as indicated by luciferase reporter assays. In conclusion, miR-455-3p may activate early chondrogenesis by directly targeting Runx2.
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Changhe
authors.initials: C
authors.lastname: Hou
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Fangang
authors.initials: F
authors.lastname: Meng
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Ziji
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Huang
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Weishen
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address: sysumf@126.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address: liaowmsysu@163.com.
authors.forename: Weiming
authors.initials: W
authors.lastname: Liao
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D020219:Chondrogenesis / Q000235:genetics*; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D013997:Time Factors
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C574091:MIRN455 microRNA, mouse; D035683:MicroRNAs
keywords: Chondrogenesis; MiR-455-3p; Runt-related transcription factor 2