title: Macrophage infiltration promotes invasiveness of breast cancer cells via activating long non-coding RNA UCA1.
abstract: There is now considerable evidence supporting the view that macrophage infiltration is playing a critical role in the proliferation and progression of breast cancer but the underlying molecular mechanisms remain largely unknown. To this end, using long non-coding RNA (lncRNA) expression profiling, we examined changes in lncRNA expression in breast cancer cells treated with conditioned medium (CM) from cultured human THP-1 macrophages. We found that treatment with macrophage CM induced the expression of numerous lncRNAs, including urothelial cancer associated 1 (UCA1). Knockdown of UCA1 using shRNA inhibited AKT phosphorylation and abolished invasiveness of tumor cells induced by macrophage CM. Consistent with these results; we further showed that UCA1 level was significantly enhanced in human primary breast tumors and correlated with advanced clinical stage, supporting its role in promoting carcinogenesis and progression of breast cancer. Together, these results suggest that macrophage could promote invasiveness of breast cancer cells by enhancing expression of lncRNA UCA1.
authors.affiliation: Lishui Central Hospital & Zhejiang University Lishui Hospital Lishui, Zhejiang, China.
authors.forename: Shuzheng
authors.initials: S
authors.lastname: Chen
authors.affiliation: Lishui Central Hospital & Zhejiang University Lishui Hospital Lishui, Zhejiang, China.
authors.forename: Chuxiao
authors.initials: C
authors.lastname: Shao
authors.affiliation: Lishui Central Hospital & Zhejiang University Lishui Hospital Lishui, Zhejiang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.affiliation: Lishui Central Hospital & Zhejiang University Lishui Hospital Lishui, Zhejiang, China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ji
authors.affiliation: Lishui Central Hospital & Zhejiang University Lishui Hospital Lishui, Zhejiang, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Lishui Center for Disease Control and Prevention Lishui, Zhejiang, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Lishui Center for Disease Control and Prevention Lishui, Zhejiang, China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D017077:Culture Media, Conditioned; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000473:pathology*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering
chemical_list: D017077:Culture Media, Conditioned; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; C515159:UCA1 RNA, human
keywords: Macrophage; UCA1; breast cancer