title: Fluorescence-based bioassays for the detection and evaluation of food materials.
abstract: We summarize here the recent progress in fluorescence-based bioassays for the detection and evaluation of food materials by focusing on fluorescent dyes used in bioassays and applications of these assays for food safety, quality and efficacy. Fluorescent dyes have been used in various bioassays, such as biosensing, cell assay, energy transfer-based assay, probing, protein/immunological assay and microarray/biochip assay. Among the arrays used in microarray/biochip assay, fluorescence-based microarrays/biochips, such as antibody/protein microarrays, bead/suspension arrays, capillary/sensor arrays, DNA microarrays/polymerase chain reaction (PCR)-based arrays, glycan/lectin arrays, immunoassay/enzyme-linked immunosorbent assay (ELISA)-based arrays, microfluidic chips and tissue arrays, have been developed and used for the assessment of allergy/poisoning/toxicity, contamination and efficacy/mechanism, and quality control/safety. DNA microarray assays have been used widely for food safety and quality as well as searches for active components. DNA microarray-based gene expression profiling may be useful for such purposes due to its advantages in the evaluation of pathway-based intracellular signaling in response to food materials.
authors.affiliation: Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Kasi-i, Higashi-ku, Fukuoka 813-8503, Japan. nishi@ip.kyusan-u.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nishi
authors.affiliation: Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Kasi-i, Higashi-ku, Fukuoka 813-8503, Japan. isobe@ip.kyusan-u.ac.jp.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Isobe
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. zhu-y@aist.go.jp.|Scinet Company, 4-21-12 Takanawa, Minato-ku, Tokyo 108-0074, Japan. zhu-y@aist.go.jp.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. kiyama.r@aist.go.jp.
authors.forename: Ryoiti
authors.initials: R
authors.lastname: Kiyama
mesh_terms: D001681:Biological Assay; D005456:Fluorescent Dyes; D005504:Food Analysis; D046228:Microarray Analysis; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes
keywords: bioassay; fluorescent dye; food study; microarray; signaling pathway