title: Role of adiponectin in sphingosine-1-phosphate induced airway hyperresponsiveness and inflammation.
abstract: Epidemiological data suggest that obesity represent an important risk factor for asthma, but the link between excess fat and airway hyperresponsiveness (AHR) and inflammation is not fully understood. Recently, a key role in physiopathologic conditions of lungs has been given to adiponectin (Acrp30). Acrp30 is one of the most expressed adipokines produced and secreted by adipose tissue, showing an intriguing relationship with metabolism of sphingolipids. Sphingosine-1-phosphate (S1P) has been proposed as an important inflammatory mediator implicated in the pathogenesis of airway inflammation and asthma. In the present study we analyze the effects of recombinant Acrp30 administration in an experimental model of S1P-induced AHR and inflammation. The results show that S1P is able to reduce endogenous Acrp30 serum levels and that recombinant Acrp30 treatment significantly reduce S1P-induced AHR and inflammation. Moreover, we observed a reduction of Adiponectin receptors (AdipoR1, AdipoR2 and T-cadherin) expression in S1P treated mice. Treatment with recombinant Acrp30 was able to restore Acrp30 serum levels and adiponectin receptors expression. These results could indicate the ability of S1P to modulate the Acrp30 action, by modulating not only the serum levels of the protein, but also its receptors. Taken together, these data suggest that adiponectin could represent a possible biomarker in obesity-associated asthma.
authors.affiliation: CEINGE-Advanced Biotechnology s.c.ar.l, Naples, Italy.
authors.forename: Ersilia
authors.initials: E
authors.lastname: Nigro
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Matteis
authors.affiliation: Department of Experimental Pharmacology, University Federico II of Naples, Naples, Italy.
authors.forename: Fiorentina
authors.initials: F
authors.lastname: Roviezzo
authors.affiliation: Department of Experimental Pharmacology, University Federico II of Naples, Naples, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mattera Iacono
authors.affiliation: CEINGE-Advanced Biotechnology s.c.ar.l, Naples, Italy; Department of Molecular Medicine and Medical Biotechnology, University Federico II of Naples, Naples, Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Scudiero
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spaziano
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Gioia
authors.initials: G
authors.lastname: Tartaglione
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Konrad
authors.initials: K
authors.lastname: Urbanek
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Filosa
authors.affiliation: CEINGE-Advanced Biotechnology s.c.ar.l, Naples, Italy; Department of Environmental Sciences and Technologies Biological and Pharmaceutical, Second University of Naples, Caserta, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: Daniele
authors.affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy. Electronic address: bruno.dagostino@unina2.it.
authors.forename: Bruno
authors.initials: B
authors.lastname: D'Agostino
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000378:metabolism*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D015820:Cadherins; C099972:H-cadherin; D018793:Interleukin-13; D008246:Lysophospholipids; D054419:Receptors, Adiponectin; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D015847:Interleukin-4; C060506:sphingosine 1-phosphate; D013110:Sphingosine
keywords: Adiponectin; Adiponectin receptors; Airway hyperresponsiveness; Inflammation; S1P; Sphingosine 1-phosphate (PubChem CID: 5283560)