title: Various instrumental approaches for determination of organic acids in wines.
abstract: Biosensors based on lactate oxidase, sarcosine oxidase and mixture of fumarase and sarcosine oxidase were used for monitoring of organic acids in wine samples. Additionally, tartaric acid was determined by modified colorimetric method based on formation of the vanadate-tartrate complex. The above mentioned methods were used for the analysis of 31 wine samples and obtained data were compared with the results from capillary electrophoresis as a basic standard method. This comparison showed a certain degree of correlation between biosensors and capillary electrophoresis. The provided information pointed to the potential uses of biosensors in the field of winemaking.
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic. Electronic address: zery@centrum.cz.
authors.forename: Jiri
authors.initials: J
authors.lastname: Zeravik
authors.affiliation: CEITEC - Central European Institute of Technology, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Fohlerova
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Miodrag
authors.initials: M
authors.lastname: Milovanovic
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Kubesa
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Zeisbergerova
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Lacina
authors.affiliation: Department of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade-Zemun, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petrovic
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Glatz
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Skladal
mesh_terms: D000143:Acids / Q000032:analysis*; D000818:Animals; D001407:Bacillus / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D003124:Colorimetry / Q000379:methods*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods; D005285:Fermentation; D005504:Food Analysis / Q000295:instrumentation* / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry; D008293:Malates / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000737:chemistry; D009206:Myocardium / Q000201:enzymology; D009930:Organic Chemicals / Q000032:analysis; D010376:Pediococcus / Q000201:enzymology; D011108:Polymers / Q000737:chemistry; D050879:Sarcosine Oxidase / Q000737:chemistry; D013552:Swine; D013644:Tartrates / Q000737:chemistry; D014920:Wine / Q000032:analysis*
chemical_list: D000143:Acids; D008293:Malates; D009930:Organic Chemicals; D011108:Polymers; D013644:Tartrates; D019344:Lactic Acid; C030298:malic acid; D006899:Mixed Function Oxygenases; C021497:lactate 2-monooxygenase; D050879:Sarcosine Oxidase; C029768:tartaric acid
keywords: Biosensor; Capillary electrophoresis; Carboxylic acids; Wine