title: Potential in two types of collagen scaffolds for urological tissue engineering applications - Are there differences in growth behaviour of juvenile and adult vesical cells?
abstract: The aging society has a deep impact on patient care in urology. The number of patients in need of partial or whole bladder wall replacement is increasing simultaneously with the number of cancer incidents. Therefore, urological research requires a model of bladder wall replacement in adult and elderly people. Two types of porcine collagen I/III scaffolds were used in vitro for comparison of cell growth of two different pig breeds at different growth stages. Scaffolds were characterised with scanning electron and laser scanning microscopy. Urothelial and detrusor smooth muscle cells were isolated from 15 adult Göttingen minipigs and 15 juvenile German Landrace pigs. Growth behaviour was examined in cell culture and seeded onto the collagen scaffolds via immunohistochemistry, two-photon laser scanning microscopy and a viability assay. The collagen scaffolds showed different structured surfaces which are appropriate for seeding of the two different cell types. Moisturisation of the scaffolds resulted in a change of the structure. Cell growth of German Landrace urothelial cells and smooth muscle cells was significantly higher than cell growth of the Göttingen minipig cells. Seeding of scaffolds with both cell types from both pig races was possible which could be shown by immunohistochemistry and two-photon laser scanning microscopy. Growth behaviour on the scaffolds was significantly increased for the German Landrace compared to Göttingen minipig. Nevertheless, seeding with the adult Göttingen minipig cells resulted in a closed layer on the surface and urothelial cells and smooth muscle cells showed increasing growth until day 14. The results show that these collagen scaffolds are adequate for the seeding with vesical cells. Moreover, they seem appropriate for the use as an in vitro model for the adult or elderly as the cells of the adult Göttingen minipig too, show good growth behaviour.
authors.affiliation: Department of Urology, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Leonhäuser
authors.affiliation: Interdisciplinary Center for Clinical Research IZKF Aachen, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Vogt
authors.affiliation: Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University Hospital, Aachen, Germany.
authors.forename: R H
authors.initials: RH
authors.lastname: Tolba
authors.affiliation: Department of Urology, RWTH Aachen University Hospital, Aachen, Germany jgrosse@ukaachen.de.
authors.forename: J O
authors.initials: JO
authors.lastname: Grosse
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D012685:Sepharose / Q000737:chemistry; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D001743:Urinary Bladder / Q000473:pathology; D019459:Urothelium / Q000378:metabolism
chemical_list: D003094:Collagen; D012685:Sepharose
keywords: German Landrace/Göttingen minipig; Urinary bladder; collagen scaffold; in vitro; juvenile vs. adult patient; large animal model; urothelial/smooth muscle cells