title: Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
abstract: Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells' genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.|Department of Biosciences and Informatics, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Ojima
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.|Department of Biosciences and Informatics, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Shibata
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.|Department of Biosciences and Informatics, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Saito
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.|Department of Research Team for Geriatric Medicine, Tokyo Metropolitan Institute of Gerontology, Tokyo, 173-0015, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyoda
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakajima
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Yamazaki-Inoue
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyagawa
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Jun-Ichiro
authors.initials: JI
authors.lastname: Fujimoto
authors.affiliation: Department of Biosciences and Informatics, Keio University, Kanagawa, 223-8522, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sato
authors.affiliation: National Research Institute for Child Health and Development, Tokyo, 157-8535, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics; D015415:Biomarkers; D002454:Cell Differentiation; D002460:Cell Line; D002853:Chromatography, Liquid; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D014644:Genetic Variation; D005838:Genotype; D006017:Glycolipids / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D053719:Tandem Mass Spectrometry
chemical_list: D000017:ABO Blood-Group System; D015415:Biomarkers; D006017:Glycolipids; D006028:Glycosphingolipids; D011134:Polysaccharides