title: CHI3L1 nuclear localization in monocyte derived dendritic cells.
abstract: Chitinase-3-like-1 protein (CHI3L1) is a glycosyl hydrolase (GH) highly expressed in a variety of inflammatory diseases at infectious and non-infectious etiology. CHI3L1 is produced by a wide variety of cells including monocyte-derived macrophages cell lines such as polarized M1 and M2 type macrophages, osteoclasts and Kupffer cells. In this study we have examined the expression of CHI3L1 during the differentiation and maturation of dendritic cells. Magnetically-isolated peripheral blood monocytes were differentiated toward immature DCs (iDC) and mature DCs (mDCs) through a combination of factors and cytokines. Our result showed, for the first time, that CHI3L1 is expressed during the process of differentiation and maturation of dendritic cells in time dependent manner. Furthermore, the CHI3L1 is evenly distributed in cytoplasm and in the nucleus of both the iDCs and mDCs. These results suggest that CHI3L1 may play crucial role in the DCs immunoresponse.
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy. Electronic address: mdirosa@unict.it.
authors.forename: Michelino
authors.initials: M
authors.lastname: Di Rosa
authors.affiliation: Division of Haematology, AOU "Policlinico-Vittorio Emanuele", University of Catania, Catania, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Tibullo
authors.affiliation: Department of Biological, Geological and Environmental Sciences, University of Catania, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Saccone
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.
authors.forename: Gisella
authors.initials: G
authors.lastname: Distefano
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.
authors.forename: Maria Sofia
authors.initials: MS
authors.lastname: Basile
authors.affiliation: Division of Haematology, AOU "Policlinico-Vittorio Emanuele", University of Catania, Catania, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Di Raimondo
authors.affiliation: Department of Biomedical and Biotechnology Sciences, University of Catania, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Malaguarnera
mesh_terms: D054392:Adipokines / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D000071451:Chitinase-3-Like Protein 1; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D037102:Lectins / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D061251:Primary Cell Culture; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
chemical_list: D054392:Adipokines; C084533:CHI3L1 protein, human; D000071451:Chitinase-3-Like Protein 1; C508598:IL4 protein, human; D037102:Lectins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: CHI3L1; Immunofluorescence; Monocyte derived dendritic cells; YKL40