title: [Significance of TSLC1 gene methylation and TSLC1 protein expression in the progression of cervical lesions].
abstract: OBJECTIVE
  To study the expression and significance of tumor suppressor in lung cancer 1 (TSLC1) gene methylation, the expression of TSLC1 protein in cervix cancer and precancerous lesions as well as their relationship with HR-HPV DNA infection.
  
  
  METHODS
  The clinicopathological data of 92 cases of different cervical lesions during March 2011 to August 2012 treated in our hospital were collected. There were pathologically confirmed 10 cases of normal cervix, 26 cases of cervical intraepithelial neoplasia (CIN) I, 20 cases of CIN II, 15 cases of CIN III, and 21 cases of cervical cancer. Methylation-specific polymerase chain reaction (MSP) was used to detect the TSLC1 gene methylation status in cervical lesions, immunohistochemistry (SP) was used to detect the expressions of TSLC1 protein in cervical lesions, and the second generation hybrid capture (HC2) method was used to detect the high-risk HPV in cervical lesions.
  
  
  RESULTS
  The expression rate of TSLC1 gene methylation in normal cervical tissue, CIN I, CIN II, CIN III and SCC were 10.0%, 30.8%, 55.0%, 60.0%, 66.7%, respectively, showing a statistically significant difference (P = 0.004). The positive expression rate of TSLC1 protein in normal cervical tissue, CIN I, CIN II, CIN III and SCC were 100.0%, 80.8%, 65.0%, 33.3%, and 23.8%, respectively, with a significant difference (P = 0.004). In the progression from CIN to invasive cervical cancer, there was no significant correlation between TSLC1 gene methylation and HR-HPV DNA infection (P = 0.919), TSLC1 protein expression and HR-HPV DNA infection (P = 0.664). The correlation analysis showed a negative correlation between TSLC1 gene methylation and TSLC1 protein expression (r = -0.674, P < 0.001).
  
  
  CONCLUSIONS
  TSLC1 gene promoter methylation may be an early event in the cervical carcinogenesis, become an early sensitive marker, and serve the early prevention and prognostic prediction for cervical cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School, Beijing 100853, China.
authors.forename: Xuye
authors.initials: X
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.forename: Yaling
authors.initials: Y
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Liwu
authors.initials: L
authors.lastname: Xie
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Xia
authors.forename: Jinsong
authors.initials: J
authors.lastname: Du
authors.forename: Linping
authors.initials: L
authors.lastname: Wei
authors.affiliation: Email: zlxy79680@126.com.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
mesh_terms: D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D018450:Disease Progression; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D008745:Methylation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism; D002578:Uterine Cervical Dysplasia / Q000235:genetics / Q000378:metabolism
chemical_list: C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins