title: Isolation and Characterization of an Endophytic Fungal Strain with Potent Antimicrobial and Termiticidal Activities From Port-Orford-Cedar.
abstract: Termites are responsible for an estimated US$1 billion annually in property damage, repairs, pest control, and prevention. There is an urgent need of finding a better alternative way to control and prevent termites. Port-Orford-Cedar (POC) has been known to have significant levels of natural durability and termiticidal activities due to its extractive contents. In this study, 25 endophytes including 22 fungal and 3 bacterial strains were isolated from the POC. Four strains, namely, HDZK-BYF21, HDZK-BYF1, HDZK-BYF2, and HDZK-BYB11, were chosen to test their termiticidal activities. The fermentation broth of strain HDZK-BYF21 displayed the potent antimicrobial and termiticidal activities. Morphological examination and 18 S rDNA sequence analysis demonstrated that strain HDZK-BYF21 belonged to the genus Aspergillus. This finding indicates the existence of an interesting chemical symbiosis between an endophytic fungus and its host. This is also the first report on endophytes isolated from the POC that may have potential termiticidal activities. Endophytes with termiticidal activities can be grown in bioreactor to provide an inexhaustible supply of bioactive compounds and thus can be exploited commercially.
authors.affiliation: School of Life Science, Heilongjiang University, Harbin 150080, China. School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Chung-Yun
authors.initials: CY
authors.lastname: Hse
authors.affiliation: Southern Research Station, Adhesive and Composite Laboratory, Pineville, LA 71360.
authors.forename: Todd
authors.initials: T
authors.lastname: Shupe
authors.affiliation: School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Mingjing
authors.initials: M
authors.lastname: Sun
authors.affiliation: School of Life Science, Heilongjiang University, Harbin 150080, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science, Heilongjiang University, Harbin 150080, China. zybin395@126.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000502:physiology*; D029779:Cupressaceae / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D060026:Endophytes / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000502:physiology; D007306:Insecticides / Q000494:pharmacology*; D020049:Isoptera / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D000890:Anti-Infective Agents; D004271:DNA, Fungal; D007306:Insecticides; D012337:RNA, Ribosomal, 18S
keywords: antitermiticidal activity; biocontrol; endophyte; fermentation metabolite; termite