title: Magnetic-graphene based molecularly imprinted polymer nanocomposite for the recognition of bovine hemoglobin.
abstract: The protein imprinted technique combining surface imprinting and nanomaterials has been an attractive strategy for recognition and rapid separation of proteins. In this work, magnetic-graphene (MG) was chosen as the supporting substrate for the magnetic nanomaterials, which served to absorb the targeting imprinting molecules, bovine hemoglobin (BHb). Acryl amide (AAm) with a high affinity to BHb and N,N'- methylenebisacrylamide (MBA) were selected as the functional monomer and cross-linking agent, respectively. After in-situ polymerization, the proposed magnetic-graphene based molecularly imprinted polymer (MG-MIP) was obtained with a further extraction step of imprinted BHb. Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), raman spectroscopy(RS), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM) were employed to characterize the resulted MG-MIP. The maximum adsorption capability (Qmax) was determined by Langmuir Isotherm Plots and was 186.73 mg/g for imprinted nanomaterials (MIP) with an imprinting factor of 1.96. The selectivity of MG-MIP was investigated by using several proteins that are different in molecular mass and isoelectric points as the reference. The results showed that the shape memory effect of imprinted cavities, the size of proteins and the charge effect of proteins were the major factors for the selective recognition. The proposed method was also employed to specifically capture BHb from a binary protein mixture.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China. Electronic address: wyzss@hnu.edu.cn.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Yanjin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Cenjin
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, College of Basic Medicine, Central South University, Changsha 410083, PR China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D006108:Graphite / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D054802:Molecular Imprinting; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D011108:Polymers; D006108:Graphite
keywords: Bovine hemoglobin; Graphene; Magnetic nanoparticle; Surface imprinting