title: Generation and Selection of Orf Virus (ORFV) Recombinants.
abstract: Orf virus (ORFV) is an epitheliotropic poxvirus, which belongs to the genus Parapoxvirus. Among them the highly attenuated, apathogenic strain D1701-V is regarded as a promising candidate for novel virus vector vaccines. Our recent work demonstrated that those ORFV-based recombinants were able to induce protective, long-lasting immunity in various hosts that are non-permissive for ORFV. In this chapter we describe procedures for the generation, selection, propagation, and titration of ORFV recombinants as well as transgene detection by PCR or immunohistochemical staining.
authors.affiliation: Institute of Immunology, Friedrich-Loeffler-Institute, Südufer 10, Island of Riems, Greifswald, Germany. achim.rziha@fli.bund.de.|Department of Immunology, Interfaculty Institute of Cell Biology, Eberhard Karls Universität, Auf der Morgenstelle 15, Tübingen, 72076, Germany. achim.rziha@fli.bund.de.
authors.forename: Hanns-Joachim
authors.initials: HJ
authors.lastname: Rziha
authors.affiliation: Institute of Immunology, Friedrich-Loeffler-Institute, Südufer 10, Island of Riems, Greifswald, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Rohde
authors.affiliation: Institute of Immunology, Friedrich-Loeffler-Institute, Südufer 10, Island of Riems, Greifswald, Germany.|Department of Immunology, Interfaculty Institute of Cell Biology, Eberhard Karls Universität, Auf der Morgenstelle 15, Tübingen, 72076, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Amann
mesh_terms: D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D009923:Orf virus / Q000235:genetics* / Q000276:immunology; D018157:Parapoxvirus / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines
keywords: Orf virus (ORFV); Parapoxvirus; Recombinant vector vaccine