title: Two Chitin Biosynthesis Pathway Genes in Bactrocera dorsalis (Diptera: Tephritidae): Molecular Characteristics, Expression Patterns, and Roles in Larval-Pupal Transition.
abstract: Glucose-6-phosphate isomerase (G6PI) and UDP-N-acetylglucosamine pyrophosphorylase (UAP), two key components in the chitin biosynthesis pathway, are critical for insect growth and metamorphosis. In this study, we identified the genes BdG6PI and BdUAP from the oriental fruit fly, Bactrocera dorsalis (Hendel). The open reading frames (ORFs) of BdG6PI (1,491 bp) and BdUAP (1,677 bp) encoded 496 and 558 amino acid residues, respectively. Multiple sequence alignments showed that BdG6PI and BdUAP had high amino acid sequence identity with other insect homologues. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated that BdG6PI was mainly expressed in the early stages of third-instar larvae and adults, while significantly higher expression of BdUAP was observed in adults. Both transcripts were expressed highly in the Malpighian tubules, but only slightly in the tracheae. The expression of both BdG6PI and BdUAP was significantly up-regulated by 20-hydroxyecdysone exposure and down-regulated by starvation. Moreover, injection of double-stranded RNAs of BdG6PI and BdUAP into third-instar larvae significantly reduced the corresponding gene expressions. Additionally, silencing of BdUAP resulted in 65% death and abnormal phenotypes of larvae, while silencing of BdG6PI had a slight effect on insect molting. These findings provide some data on the roles of BdG6PI and BdUAP in B. dorsalis and demonstrate the potential role for BdUAP in larval-pupal transition.
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China. College of Biology and Environmental Engineering, Guiyang University, Guiyang, Guizhou 550005, P. R. China. wangjinjun@swu.edu.cn.
authors.forename: Wen-Jia
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Yi-Bei
authors.initials: YB
authors.lastname: Wu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China. College of Biology and Environmental Engineering, Guiyang University, Guiyang, Guizhou 550005, P. R. China.
authors.forename: Kang-Kang
authors.initials: KK
authors.lastname: Xu
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Xie
authors.affiliation: Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, P. R. China. wangjinjun@swu.edu.cn.
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004441:Ecdysterone / Q000378:metabolism; D005508:Food Deprivation; D015870:Gene Expression; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008675:Metamorphosis, Biological; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010802:Phylogeny; D011679:Pupa / Q000235:genetics / Q000254:growth & development; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D033621:Tephritidae / Q000235:genetics* / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; D004441:Ecdysterone; D009713:Nucleotidyltransferases; C023226:UDPacetylglucosamine pyrophosphorylase; D005956:Glucose-6-Phosphate Isomerase
keywords: Bactrocera dorsalis; RNA interference; UDP-N-acetylglucosamine pyrophosphorylase; expression pattern; glucose-6-phosphate isomerase