title: Design, Synthesis, and Characterization of Small-Molecule Reagents That Cooperatively Provide Dual Readouts for Triaging and, When Necessary, Quantifying Point-of-Need Enzyme Assays.
abstract: A newly designed small molecule reagent provides both qualitative and quantitative readouts in assays that detect enzyme biomarkers. The qualitative readout enables rapid triaging of samples so that only samples that contain relevant concentrations of the target analyte must be quantified. The reagent is accessible in essentially three steps and 34% overall yield, is stable as a solid when heated to 44 °C for >1 month, and does not produce background signal when used in an assay. This paper describes the design and synthesis of the reagent, characterizes its response properties, and establishes the scope of its reactivity.
authors.affiliation: Department of Chemistry, Pennsylvania State University , University Park, Pennsylvania 16802, United States.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Brooks
authors.affiliation: Department of Chemistry, Pennsylvania State University , University Park, Pennsylvania 16802, United States.
authors.forename: Hemakesh
authors.initials: H
authors.lastname: Mohapatra
authors.affiliation: Department of Chemistry, Pennsylvania State University , University Park, Pennsylvania 16802, United States.
authors.forename: Scott T
authors.initials: ST
authors.lastname: Phillips
mesh_terms: D001681:Biological Assay; D003374:Coumarins / Q000737:chemistry*; D057075:Enzyme Assays; D005453:Fluorescence; D007202:Indicators and Reagents / Q000138:chemical synthesis* / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D003374:Coumarins; D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; C007638:ethanethiol