title: MicroRNA-219-5p functions as a tumor suppressor partially by targeting platelet-derived growth factor receptor alpha in colorectal cancer.
abstract: Platelet-derived growth factor receptor (PDGFR) signaling pathway was involved in the progress of colorectal cancer (CRC). By using the bioinformatic system online, we found that PDGFRα is a potential target of miR-219-5p. However, the expression pattern and underlying mechanisms of miR-219-5p had not been elucidated in CRC. Herein, we first evaluated the expression of miR-219-5p in tumor tissues by real-time polymerase chain reaction. Next, we confirmed that PDGFRα is the target of miR-219-5p by using luciferase report. And then, we investigated the biological functions of miR-219-5p in vitro in cell proliferation and apoptosis as well as cell cycle by gain and loss of function strategies. Data shown that miR-219-5p is down-regulated in CRC tissues compared with the corresponding matched normal tissues. PDGFRα was a direct target of miR-219-5p. Overexpression of miR-219-5p could inhibit cell proliferation, promote cell apoptosis and induce cell cycle arrest at the G1 phase. Furthermore, miR-219-5p suppressed the activation of the phosphatidylinositol 3-kinase/Akt signaling pathway and downregulated G1 cell-cycle-related protein cyclin D1, cyclin-dependent kinase (CDK) 4, and CDK6. Taken together, our results demonstrate that miR-219-5p functions as a tumor suppressor partially by targeting PDGFRα in colorectal cancer.
authors.forename: G B
authors.initials: GB
authors.lastname: Xiong
authors.forename: G N
authors.initials: GN
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Xiao
authors.forename: B Z
authors.initials: BZ
authors.lastname: Niu
authors.forename: H Z
authors.initials: HZ
authors.lastname: Qiu
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: G L
authors.initials: GL
authors.lastname: Lin
authors.forename: L
authors.initials: L
authors.lastname: You
authors.forename: H
authors.initials: H
authors.lastname: Shu
keywords: PDGFRα; PI3K/Akt.; colorectal cancer; miR-219-5p; microRNA