title: Anti-biofilm effect of nanometer scale silver (NmSAg) coatings on glass and polystyrene surfaces against P. mirabilis, C. glabrata and C. tropicalis strains.
abstract: OBJECTIVE
  Nowadays, in order to terminate biofilm associated infections, coating of particular biomaterial surfaces with particular substances, via some nanotechnological tools, is being applied. Therefore, in the present study, investigation of anti-biofilm effects of nanometer scale silver (NmSAg) coatings on glass and polystyrene surfaces against clinical strains of Proteus mirabilis, Candida glabrata and Candida tropicalis was aimed.
  
  
  METHODS
  In this study, glass and polystyrene slabs with 1.5 cm × 1.5 cm × 0.3 mm dimensions were cleaned by using surface plasma technology, covered with NmSAg by using a physical vapor deposition machine, and biofilm inhibition was determined by crystal violet binding assay.
  
  
  RESULTS
  According to our results, 32 nm of silver layer on a glass slab decreased biofilm formation of P. mirabilis strain to a maximum amount of 88.1% and caused 20.9% inhibition in biofilm formation of C. glabrata strain. On the other hand, NmS coating of Ag on a polystyrene slab caused 34.4% and 20% inhibitions, respectively, in biofilm formations of C. glabrata and C. tropicalis strains. Although biofilm inhibition of NmSAg layer on polystyrene slab was more (34.4%) than biofilm inhibition caused by NmSAg layer on glass slab (20.9%), C. glabrata strain's biofilm formation on uncoated glass slab was lower than both uncoated and NmSAg-coated polystyrene slabs.
  
  
  CONCLUSIONS
  Our results show that glass surfaces with NmSAg coatings can be used as a new surface material of various indwelling devices on which P. mirabilis colonizations frequently occur and in order to avoid C. glabrata-associated biofilm infections, it is more useful to choose a surface material of glass rather than choosing a surface material of polystyrene.
authors.affiliation: Biology Department, Biotechnology, Hacettepe University, Beytepe, Ankara - Turkey.
authors.forename: Gulcan
authors.initials: G
authors.lastname: Sahal
authors.affiliation: Chemical Engineering Department and Bioengineering Division, Centre for Bioengineering and Biyomedtek/Nanobiyomedtek Hacettepe University, Beytepe, Ankara - Turkey.
authors.forename: Behzad
authors.initials: B
authors.lastname: Nasseri
authors.affiliation: Biology Department, Biotechnology, Hacettepe University, Beytepe, Ankara - Turkey.
authors.forename: Isil Seyis
authors.initials: IS
authors.lastname: Bilkay
authors.affiliation: Chemical Engineering Department and Bioengineering Division, Centre for Bioengineering and Biyomedtek/Nanobiyomedtek Hacettepe University, Beytepe, Ankara - Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Piskin
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D041221:Candida glabrata / Q000187:drug effects / Q000502:physiology*; D041022:Candida tropicalis / Q000187:drug effects / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity; D011137:Polystyrenes / Q000737:chemistry*; D011513:Proteus mirabilis / Q000187:drug effects / Q000502:physiology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D012834:Silver