title: Down-expression of miR-152 lead to impaired anti-tumor effect of NK via upregulation of HLA-G.
abstract: It is known that chronic HBV infection (CHB) is the major risk factor for hepatocellular carcinoma (HCC) because CHB could not only cause liver tumorigenesis but also lead to change of local microenviroment and lower immune response to infected and cancerous cells (immune tolerance). Human leucocyte antigen-G (HLA-G) belongs to a non-classic MHC-I family and was considered to be an immune tolerance molecule, which could bind to immunosuppressive receptors of natural killer cell (NK) and T cells and trigger immunosuppressive signaling. Recently, numerous studies highlighted that microRNAs (miRNAs) were significantly differentially expressed in HCC tumorigenesis, and the expression was tissue-specific, indicating that miRNAs may cause great epigenetic changes in HCC tumorigenesis. In this study, we found that the expression of HLA-G was upregulated by hepatitis B virus (HBV) infection and miR-152; a HLA-G-targeting miRNA was downregulated by HBV infection. And high expression of HLA-G further suppressed NK against cancer cells, providing a new concept that miR-152 was involved in HBV-induced hepatocellular carcinoma.
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.|Department of Clinical Laboratory, Weifang People's Hospital, Shandong, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Bian
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.|Department of Clinical Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Yuanquan
authors.initials: Y
authors.lastname: Si
authors.affiliation: Department of Medicine, Shandong Provincial Chest Hospital, Jinan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Guixi
authors.initials: G
authors.lastname: Zheng
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China.
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan, China. yizhang@sdu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D059951:HLA-G Antigens / Q000235:genetics* / Q000378:metabolism; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D059951:HLA-G Antigens; C550856:MIRN152 microRNA, human; D035683:MicroRNAs
keywords: HBV; HLA-G; Immune tolerance; miR-152