title: Epithelial-mesenchymal transition in patients of pulmonary adenocarcinoma: correlation with cancer stem cell markers and prognosis.
abstract: Adenocarcinoma is the most common histologic type of non-small cell lung carcinomas. The existence of lung cancer stem cells (CSCs) and epithelial-mesenchymal transition (EMT) in human tissue is controversy. The aim of this study is to investigate the expression and clinical significance of CSCs and EMT markers and evaluate the correlation between the two in lung adenocarcinoma. A total of 97 cases comprise the tissue microarray from surgical resection for primary lung adenocarcinoma. Immunohistochemistry for ALDH1 and CD44 as CSC markers and E-cadherin, vimentin, fibronectin, SMA as EMT markers was performed. High ALDH1A1 expression was statistically associated with female gender (P=0.001), smoker (P=0.012), and high pT stages (P=0.046). High CD44 expression was statistically associated with female gender (P=0.008), non-smoker (P=0.000), and no pleural invasion (P=0.039). High expression of ALDH1 was associated with good overall survival (P=0.021). High expression of CD44 was correlated with both good overall survival (P=0.024) and disease-free survival (P=0.000). Vimentin expression was associated with pT stage (P=0.001) and pleural invasion (P=0.028). E-cadherin, fibronectin and SMA were not associated with clinicopathologic correlation and all EMT markers were not correlated with survival of lung adenocarcinoma. CSC markers expression was not related to EMT. Our results showed that the expression of CSCs was associated with a good prognosis in lung adenocarcinoma. The prognostic significance of EMT markers was skeptical in this study. There is a need for more research about CSC, EMT, and the relation between these two in human lung adenocarcinoma.
authors.affiliation: Department of Pathology, Catholic University of Daegu College of Medicine Daegu, Republic of Korea.
authors.forename: Woo Jung
authors.initials: WJ
authors.lastname: Sung
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Catholic University of Daegu College of Medicine Daegu, Republic of Korea.
authors.forename: Ki-Sung
authors.initials: KS
authors.lastname: Park
authors.affiliation: Department of Medical Statistics, Catholic University of Daegu College of Medicine Daegu, Republic of Korea.
authors.forename: Sang Gyu
authors.initials: SG
authors.lastname: Kwak
authors.affiliation: Department of Internal Medicine, Catholic University of Daegu College of Medicine Daegu, Republic of Korea.
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Hyun
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine Seoul, Republic of Korea.
authors.forename: Jae Seok
authors.initials: JS
authors.lastname: Jang
authors.affiliation: Department of Pathology, Catholic University of Daegu College of Medicine Daegu, Republic of Korea.
authors.forename: Kwan-Kyu
authors.initials: KK
authors.lastname: Park
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D000080924:Aldehyde Dehydrogenase 1 Family; D014408:Biomarkers, Tumor / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D050697:Retinal Dehydrogenase / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D005353:Fibronectins; D018960:Hyaluronan Receptors; D007527:Isoenzymes; D014746:Vimentin; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase
keywords: Lung cancer; adenocarcinoma; cancer stem cell; epithelial-mesenchymal transition; immunohistochemistry; prognosis