title: Common genetic polymorphisms in pre-microRNAs and risk of bladder cancer.
abstract: BACKGROUND
  At present, inconsistent association between single nucleotide polymorphism (SNP) in pre-miRNAs (hsa-mir-196a2 rs11614913 C/T, hsa-mir-499 rs3746444 A/G, and hsa-mir-146a rs2910164 C/G) and bladder cancer were obtained in limited studies. We performed a case-control study to test whether these three common polymorphisms are associated with bladder cancer. One hundred fifty-nine patients affected by bladder cancer and 298 unrelated healthy subjects were enrolled in the study.
  
  
  METHODS
  Using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP), genotypes of these three SNPs were determined, and their associations with bladder cancer, as well as with clinic pathological factors, and tumor progression were analyzed.
  
  
  RESULTS
  No association between bladder cancer risk and variant allele of hsa-mir-196a2 rs11614913 C/T, hsa-mir-499 rs3746444 A/G, or hsa-mir-146a rs2910164 C/G was observed. Heterozygous genotype (CT genotype) of rs11614913 was associated with a significantly decreased bladder cancer risk (P = 0.004, OR = 0.56, 95 % CI = 0.38-0.83). Further stratified analyses showed that rs2910164 is associated with the tumor stage in a recessive model and with metastasis in a dominant model (P = 0.012, OR = 0.20, 95 % CI = 0.05-0.72 and P = 0.04, OR = 2.63, 95 % CI = 1.03-6.67, respectively). No association between hsa-mir-499 rs3746444 A/G and bladder cancer was observed.
  
  
  CONCLUSIONS
  Our results suggested hsa-mir-196a2 rs11614913 C/T is associated with a significantly decreased risk of bladder cancer and hsa-mir-146a rs2910164 GG genotype is associated with clinical stage and metastasis in bladder cancer.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, 37# Guoxuexiang Street, Chengdu, 610041, PR China. dengshidiy@163.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Pathology, West China Second University Hospital, Chengdu, PR China. bearwin2000@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, 37# Guoxuexiang Street, Chengdu, 610041, PR China. lixianghuaxi@163.com.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, 37# Guoxuexiang Street, Chengdu, 610041, PR China. zhangpenghuaxi@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: C542542:MIRN146 microRNA, human; C542543:MIRN196 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs