title: Anti-inflammatory Activity of Constituents Isolated from Aerial Part of Angelica acutiloba Kitagawa.
abstract: Recently, the resources of medicinal plants have been exhausting. The root of Angelica acutiloba is one of the most important ingredients in Japanese Kampo medicine for the treatment of gynecological diseases. In our search for alternative medicinal plant resources of the root of A. acutiloba, we found that its aerial part has the anti-inflammatory potency as well as the root. Phytochemical investigation of the aerial part resulted in the isolation of four compounds including a new dimeric phthalide, namely tokiaerialide (2), along with Z-ligustilide (1), falcarindiol (3), and bergaptol (4). Next, we investigated the in vitro anti-inflammatory activity of 1-4 in lipopolysaccharide-stimulated RAW264 macrophages. Among the isolated compounds, 1 exhibited the most potent inhibition against lipopolysaccharide-induced production of prostaglandin E2 , nitric oxide, and pro-inflammatory cytokines (interleukin-6 and tumor necrosis factor-α). Compounds 3 and 4 also inhibited all inflammatory mediators, but their inhibitory abilities were weaker than those of 1. Furthermore, 1, 3, and 4 strongly also induced heme oxygenase-1. These results suggest that 1, 3, and 4 potentially exert anti-inflammatory activity, and the aerial part of A. acutiloba may be considered to be a useful medicinal resource for inflammatory diseases.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Uto
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.|School of Medicine and Pharmacy, Vietnam National University, 144 Xuan Thuy St., Cau Giay, Hanoi, Vietnam.
authors.forename: Nguyen Huu
authors.initials: NH
authors.lastname: Tung
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
authors.forename: Risa
authors.initials: R
authors.lastname: Taniyama
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
authors.forename: Tosihide
authors.initials: T
authors.lastname: Miyanowaki
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Morinaga
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D053280:Diynes / Q000302:isolation & purification / Q000494:pharmacology; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000494:pharmacology; D011564:Furocoumarins / Q000302:isolation & purification / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D064209:Phytochemicals / Q000302:isolation & purification / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D000067996:RAW 264.7 Cells; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D053280:Diynes; D005233:Fatty Alcohols; D011564:Furocoumarins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D064209:Phytochemicals; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C000608194:tokiaerialide; D009569:Nitric Oxide; C027820:ligustilide; C034379:falcarindiol; D051547:Heme Oxygenase-1; D015232:Dinoprostone; C080771:bergaptol; D015107:4-Butyrolactone
keywords: Angelica acutiloba; Z-ligustilide; aerial part; anti-inflammation; macrophages; tokiaerialide