title: Specific detection of the pesticide chlorpyrifos by a sensitive genetic-based whole cell biosensor.
abstract: The Sinorhizobium meliloti chpA promoter is highly induced in the presence of the pesticide chlorpyrifos (CPF) through the action of the transcriptional activator, ChpR. A whole-cell biosensor for the detection of CPF was developed and is composed of an Escherichia coli strain carrying a chpR expression vector and a chpA promoter-atsBA transcriptional fusion plasmid encoding sulfatase (atsA) and formylglycine generating enzyme (atsB) from Klebsiella sp. The sulfatase is posttranslationally activated by formylglycine generating enzyme (FGE) and then converts 4-methylumbelliferyl sulfate (4-MUS) to the fluorescent product, 4-methyllumbelliferone (4-MU). This biosensor system exhibited a linear response range from 25 to 500 nM CPF.
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand.
authors.forename: Wirongrong
authors.initials: W
authors.lastname: Whangsuk
authors.affiliation: Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand.
authors.forename: Sirinthra
authors.initials: S
authors.lastname: Thiengmag
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand.
authors.forename: James
authors.initials: J
authors.lastname: Dubbs
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology, Ministry of Education, Bangkok 10400, Thailand.
authors.forename: Skorn
authors.initials: S
authors.lastname: Mongkolsuk
authors.affiliation: Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand; Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology, Ministry of Education, Bangkok 10400, Thailand. Electronic address: suvit@cri.or.th.
authors.forename: Suvit
authors.initials: S
authors.lastname: Loprasert
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015374:Biosensing Techniques / Q000379:methods*; D004390:Chlorpyrifos / Q000032:analysis* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D007709:Klebsiella / Q000235:genetics; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects; D016962:Sinorhizobium meliloti / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010575:Pesticides; D004390:Chlorpyrifos
keywords: Aldehyde tag; Biosensor; Gene expression; Pesticide detection; Transcription factor