title: MiR-25 is up-regulated in non-small cell lung cancer and promotes cell proliferation and motility by targeting FBXW7.
abstract: Increasing evidence showed that miR-25 is involved in the carcinogenesis and progression of various human cancers, while its role in non-small cell lung cancer (NSCLC) is still unclear. Here, we found that miR-25 is significantly up-regulated in NSCLC tissue samples and cell lines. Inhibition of miR-25 remarkably suppressed cell proliferation, migration and invasion in NSCLC cells, whereas enforced expression of miR-25 significantly increased NSCLC cells proliferation, migration and invasion. Moreover, we identified F-box and WD repeat domain-containing 7 (FBXW7) as a direct target of miR-25 and overexpression of FBXW7 partially attenuates the oncogenic effect of miR-25 on NSCLC cells. In conclusion, miR-25 is up-regulated in NSCLC and promotes NSCLC cells proliferation and motility partially by targeting FBXW7. Our data suggest that miR-25 might serve as a potential therapeutic target for NSCLC treatment in the future.
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, China.
authors.forename: Jun-Biao
authors.initials: JB
authors.lastname: Hang
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, China.
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Che
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200025, China.
authors.forename: He-Cheng
authors.initials: HC
authors.lastname: Li
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D044783:F-Box Proteins / Q000235:genetics* / Q000378:metabolism; D000073979:F-Box-WD Repeat-Containing Protein 7; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; D044767:Ubiquitin-Protein Ligases
keywords: FBXW7; miR-25; motility; non-small cell lung cancer; proliferation