title: Cobalt doped proangiogenic hydroxyapatite for bone tissue engineering application.
abstract: The present study delineates the synthesis and characterization of cobalt doped proangiogenic-osteogenic hydroxyapatite. Hydroxyapatite samples, doped with varying concentrations of bivalent cobalt (Co(2+)) were prepared by the ammoniacal precipitation method and the extent of doping was measured by ICP-OES. The crystalline structure of the doped hydroxyapatite samples was confirmed by XRD and FTIR studies. Analysis pertaining to the effect of doped hydroxyapatite on cell cycle progression and proliferation of MG-63 cells revealed that the doping of cobalt supported the cell viability and proliferation up to a threshold limit. Furthermore, such level of doping also induced differentiation of the bone cells, which was evident from the higher expression of differentiation markers (Runx2 and Osterix) and better nodule formation (SEM study). Western blot analysis in conjugation with ELISA study confirmed that the doped HAp samples significantly increased the expression of HIF-1α and VEGF in MG-63 cells. The analysis described here confirms the proangiogenic-osteogenic properties of the cobalt doped hydroxyapatite and indicates its potential application in bone tissue engineering.
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Senthilguru
authors.initials: S
authors.lastname: Kulanthaivel
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
authors.forename: Bibhas
authors.initials: B
authors.lastname: Roy
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Tarun
authors.initials: T
authors.lastname: Agarwal
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Supratim
authors.initials: S
authors.lastname: Giri
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Krishna
authors.initials: K
authors.lastname: Pramanik
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Kunal
authors.initials: K
authors.lastname: Pal
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
authors.forename: Sirsendu S
authors.initials: SS
authors.lastname: Ray
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.affiliation: Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India. Electronic address: indraniliit@gmail.com.
authors.forename: Indranil
authors.initials: I
authors.lastname: Banerjee
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D003035:Cobalt; D017886:Durapatite
keywords: Angiogenesis; Bone tissue engineering; Cobalt; HIF-1α; Hydroxyapatite; VEGF