title: Triterpenoids and Flavonoids from the Leaves of Astragalus membranaceus and Their Inhibitory Effects on Nitric Oxide Production.
abstract: Four new cycloartane triterpenes, named huangqiyegenins V and VI and huangqiyenins K and L (1-4, resp.), together with nine known triterpenoids, 5-13, and eight flavonoids, 14-21, were isolated from a 70%-EtOH extract of Astragalus membranaceus leaves. The structures of the new compounds were elucidated by detailed spectroscopic analyses, and the compounds were identified as (9β,11α,16β,20R,24S)-11,16,25-trihydroxy-20,24-epoxy-9,19-cyclolanostane-3,6-dione (1), (9β,16β,24S)-16,24,25-trihydroxy-9,19-cyclolanostane-3,6-dione (2), (3β,6α,9β,16β,20R,24R)-16,25-dihydroxy-3-(β-D-xylopyranosyloxy)-20,24-epoxy-9,19-cyclolanostan-6-yl acetate (3), and (3β,6α,9β,16β,24E)-26-(β-D-glucopyranosyloxy)-16-hydroxy-3-(β-D-xylopyranosyloxy)-9,19-cyclolanost-24-en-6-yl acetate (4). All isolated compounds were evaluated for their inhibitory activities against LPS-induced NO production in RAW264.7 macrophage cells. Compounds 1-3, 14, 15, and 18 exhibited strong inhibition on LPS-induced NO release by macrophages with IC50 values of 14.4-27.1 μM.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).|Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Zhai
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).
authors.forename: Zhen-Ping
authors.initials: ZP
authors.lastname: Ma
authors.affiliation: College of Pharmacy, Harbin Medical University, Harbin 150081, P. R. China.|Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Chun-Juan
authors.initials: CJ
authors.lastname: Yang
authors.affiliation: Austar Pharma LLC, 18 Mayfield Ave, Edison, NJ 08837, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Pan
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Yu
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803).
authors.forename: Bing-You
authors.initials: BY
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, 24 Heping Road, Harbin 150040, P. R. China, (phone: +86-451-82193001; fax: +86-451-82110803). hxkuang@yahoo.com.
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis*; D018515:Plant Leaves / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D008070:Lipopolysaccharides; D014315:Triterpenes; D009569:Nitric Oxide
keywords: Astragalus membranaceus; Flavonoids; Nitric oxide; Triterpenoids