title: Dependency of Experimental Autoimmune Encephalomyelitis Induction on MOG35-55 Properties Modulating Matrix Metalloproteinase-9 and Interleukin-6.
abstract: Experimental autoimmune encephalomyelitis (EAE) is commonly induced with myelin oligodendrocyte glycoprotein (MOG)35-55; occasionally, EAE is not well induced despite MOG35-55 immunization. To confirm that EAE induction varies with difference in MOG35-55 properties, we compared three MOG35-55 from different commercial sources, which are MOG-A, MOG-B, and MOG-C. The peptides induced EAE disease with 100, 40, and 20 % incidence, respectively. Compared with others, MOG-A showed higher peptide purity (99.2 %) and content (92.2 %) and presented a sheet shape with additional sodium and chloride chemical elements. In MOG-A-treated group, MMP-9 activity and IL-6 levels were considerably higher than the other groups in CNS tissues, and significantly increased VCAM-1, IFN-γ, and decreased IL-4 were also shown compared to MOG-B- and/or MOG-C-treated group. In conclusion, the immunological and toxicological changes by the difference in MOG35-55 properties modulate EAE induction, and MOG35-55 which affects MMP-9 activity and IL-6 levels may be the most effective EAE-inducing antigen. This study can be potentially applied by researchers using MOG35-55 peptide and manufacturers for MOG35-55 synthesis.
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea.|Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.|Department of Biological Chemistry, Korea University of Science and Technology, Daejeon, 34113, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Seo
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea.|Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.|Department of Biological Chemistry, Korea University of Science and Technology, Daejeon, 34113, Korea.
authors.forename: Mahbub
authors.initials: M
authors.lastname: Hasan
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea.|Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Joon-Seung
authors.initials: JS
authors.lastname: Han
authors.affiliation: Advanced Analysis Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Nak-Kyoon
authors.initials: NK
authors.lastname: Kim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Kang Mi
authors.initials: KM
authors.lastname: Lee
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Ju-Hyung
authors.initials: JH
authors.lastname: Park
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Ho Jun
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Son
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea.
authors.forename: Jaeick
authors.initials: J
authors.lastname: Lee
authors.affiliation: Toxicology Laboratory, Doping Control Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea. oskwon@kist.re.kr.|Doping Control Center, Korea Institute of Science and Technology, Seoul, 02792, Korea. oskwon@kist.re.kr.|Department of Biological Chemistry, Korea University of Science and Technology, Daejeon, 34113, Korea. oskwon@kist.re.kr.
authors.forename: Oh-Seung
authors.initials: OS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology
chemical_list: D015850:Interleukin-6; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; C445081:myelin oligodendrocyte glycoprotein (35-55); D020780:Matrix Metalloproteinase 9
keywords: EAE; IL-6; Induction; MMP-9; MOG35-55; Property