title: Levels of antimüllerian hormone in serum during the normal menstrual cycle.
abstract: OBJECTIVE
  To determine whether levels of antimüllerian hormone (AMH) in serum vary during the normal menstrual cycle, using the most recently developed immunoassay method.
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  Local community.
  
  
  METHODS
  Women with normal menstrual cycles and between the ages of 18 and 45 years were recruited (n = 45). Blood samples were collected on 5 days within each cycle: two in the follicular phase and three after confirmed ovulation. Exclusion criteria were anovulatory cycles, incomplete sample collection, insufficient blood volume, or non-Caucasian ethnicity.
  
  
  METHODS
  None.
  
  
  METHODS
  Serum samples were tested for levels of AMH using a new immunoassay method (Ansh Labs). The effects of body mass index (BMI) and smoking on serum AMH levels were considered.
  
  
  RESULTS
  Serum AMH levels varied significantly during the menstrual cycle, with the highest levels in the follicular phase. When the analysis was stratified by age, AMH variation during the menstrual cycle was significant only for women older than 30 years. Serum AMH levels were not significantly altered by BMI or smoking.
  
  
  CONCLUSIONS
  The new AMH immunoassay revealed a follicular phase rise in serum levels, particularly in women over the age of 30 years. This is consistent with other reports finding an interaction of menstrual cycle variation in AMH and chronological age. Nonetheless, the extent of variation is small, and sampling on any day of the menstrual cycle is expected to adequately reflect ovarian reserve.
  
  
  BACKGROUND
  NCT01337999.
authors.affiliation: Department of Pathology and Laboratory Medicine and Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island; Center for Biomarkers and Emerging Technology, Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island. Electronic address: gmesserlian@wihri.org.
authors.forename: Geralyn
authors.initials: G
authors.lastname: Lambert-Messerlian
authors.affiliation: Center for Reproduction and Infertility, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island.
authors.forename: Beth
authors.initials: B
authors.lastname: Plante
authors.affiliation: Department of Pathology and Laboratory Medicine and Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island; Center for Biomarkers and Emerging Technology, Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Eklund
authors.affiliation: Division of Research, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island.
authors.forename: Christina
authors.initials: C
authors.lastname: Raker
authors.affiliation: Program in Women's Oncology, Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island; Center for Biomarkers and Emerging Technology, Department of Obstetrics and Gynecology, Women and Infant's Hospital, Alpert Medical School at Brown University, Providence, Rhode Island.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Moore
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D054304:Anti-Mullerian Hormone / Q000097:blood*; D015992:Body Mass Index; D005260:Female; D005498:Follicular Phase / Q000097:blood; D006801:Humans; D007118:Immunoassay; D008183:Luteal Phase / Q000097:blood; D008597:Menstrual Cycle / Q000097:blood*; D008875:Middle Aged; D011446:Prospective Studies; D012907:Smoking / Q000097:blood; D013997:Time Factors; D055815:Young Adult
chemical_list: D054304:Anti-Mullerian Hormone
keywords: Endocrinology; follicle development; luteal phase; müllerian inhibitory substance; ovarian reserve