title: MiR-29 Induces K562 Cell Apoptosis by Down-Regulating FoxM1.
abstract: BACKGROUND
  Leukemia seriously threatens human life and health. MicroRNAs can regulate cell growth, proliferation, and death. This article investigated the role of miR-29 on regulating leukemia cell growth, proliferation, and apoptosis.
  
  
  METHODS
  miR-29 and scramble miRNA were transfected to K562 cells. MTT assay, colony formation assay, caspase-3 activity detection, and flow cytometry were applied to test miR-29 effect on cell growth, proliferation, and apoptosis. Western blot was used to detect Forkhead box protein M1 (FoxM1) protein expression. After we transfected miR-29, K562 cells were transfected with FoxM1 siRNA to test cell apoptosis.
  
  
  RESULTS
  K562 cell growth and proliferation were inhibited after transfection with miR-29. Apoptosis phenome and caspase-3 activation were observed. FoxM1 level decreased. SiRNA FoxM1 enhanced miR-29-induced K562 cell apoptosis. FoxM1 overexpression suppressed miR-26-induced K562 cell apoptosis.
  
  
  CONCLUSIONS
  MiR-29 restrained K562 cell growth and proliferation. MiR-29 induced K562 cell apoptosis through down-regulating FoxM1.
authors.affiliation: Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors.forename: Hua
authors.initials: H
authors.lastname: Zhong
authors.affiliation: Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, China (mainland).
authors.forename: Ankui
authors.initials: A
authors.lastname: Jia
authors.affiliation: 0.
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Mo
authors.affiliation: Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang, Henan, China (mainland).
authors.forename: Chenguang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation; D015536:Down-Regulation; D004789:Enzyme Activation; D005434:Flow Cytometry; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C505407:CASP3 protein, human; D053148:Caspase 3