title: Antiviral activity of doxycycline against vesicular stomatitis virus in vitro.
abstract: Doxycycline (Dox) is a tetracycline derivative with broad-spectrum antimicrobial activities that is used as an effector substance in inducible gene-expression systems. We investigated the antiviral activity of Dox against vesicular stomatitis virus (VSV) infection in cultured H1299 cells. Dox at concentrations of 1.0-2.0 μg ml(-1) significantly inhibited VSV replication and the VSV-induced cytopathic effect in dose-dependent manners, suggesting that Dox may have broader activity in inhibiting viral replication, in addition to its well-defined bacteriostatic activity. Dox exerted its antiviral effect at the early-mid stage of VSV infection, suggesting that it did not interfere with VSV infectivity, adsorption, or entry into target cells. These results indicate that Dox can inhibit VSV infection and may therefore have potential applications for the treatment of viral infections.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Zhuan-Chang
authors.initials: ZC
authors.lastname: Wu
authors.affiliation: College of Life Science, Linyi University, Shuangling Road, Linyi City, Shandong 276005, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Jian-Chao
authors.initials: JC
authors.lastname: Wei
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Li
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Shao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Li
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Shi
authors.affiliation: Key Laboratory of Animal Diseases Diagnosis and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Qiu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue road, Shanghai 200241, PR China zhiyongma@shvri.ac.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ma
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004318:Doxycycline / Q000494:pharmacology*; D058492:Drug Repositioning; D006801:Humans; D008826:Microbial Sensitivity Tests; D018116:Vesiculovirus / Q000187:drug effects* / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004318:Doxycycline
keywords: antiviral activity; doxycycline; vesicular stomatitis virus