title: MiR-429 regulates gastric cancer cell invasiveness through ZEB proteins.
abstract: MicroRNAs (miRNAs) play an essential role in the tumorigenesis of gastric carcinoma (GC). MiR-429 has been recently reported to inhibit GC growth; however, whether miR-429 may also regulate the invasion of GC cells is unknown. Here, we showed that miR-429 levels were significantly decreased and ZEB1 and ZEB2 levels were significantly increased in GC specimens, compared to the paired adjacent non-tumor gastric tissue. Moreover, the levels of miR-429 and ZEB1 or ZEB2 inversely correlated in GC specimens. Bioinformatics analyses showed that miR-429 targeted the 3'-untranslated region of both ZEB1 and ZEB2 mRNA to inhibit their translation, which was confirmed by luciferase reporter assay. Overexpression of miR-429 inhibited ZEB1/2-mediated cell invasiveness, while depletion of miR-429 augmented it. Together, our data suggest that miR-429 suppression in GC promotes ZEB1/2-mediated cancer cell invasion.
authors.affiliation: Department of General Surgery, The Bethune International Peace Hospital, 398 Zhongshan West Road, Shijiazhuang, 050082, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Pathology, The Bethune International Peace Hospital, Shijiazhuang, 050082, China.
authors.forename: Jie
authors.initials: J
authors.lastname: An
authors.affiliation: Department of General Surgery, The Bethune International Peace Hospital, 398 Zhongshan West Road, Shijiazhuang, 050082, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of General Surgery, The Bethune International Peace Hospital, 398 Zhongshan West Road, Shijiazhuang, 050082, China. hou64huichi@163.com.
authors.forename: Huichi
authors.initials: H
authors.lastname: Hou
keywords: Gastric carcinoma (GC); ZEB1; ZEB2; miR-429