title: Detection of cytoplasmic proteins from Helicobacter pylori in Colony Lift Immunoassay.
abstract: Use of the Colony Lift Immunoassay has been described for several Gram negative bacteria of medical interest. In all cases detection was limited to the use of antibodies against outer membrane proteins. Here we describe the adaptation of this method for detection of the cytoplasmic CagA toxin from Helicobacter pylori.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany.
authors.forename: Diana F
authors.initials: DF
authors.lastname: Rojas-Rengifo
authors.affiliation: Molecular Diagnostics and Bioinformatics Laboratory, Biological Sciences Department, Los Andes University, Carrera 1 # 18A-10, Bogotá, Colombia.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Jaramillo
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Haas
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstrasse 9a, D-80336 München, Germany. Electronic address: jimenez@mvp.uni-muenchen.de.
authors.forename: Luisa F
authors.initials: LF
authors.lastname: Jiménez-Soto
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay / Q000379:methods*
chemical_list: D001426:Bacterial Proteins
keywords: CLI; Colony blot; Cytoplasmic proteins; Helicobacter pylori; Immunoassay