title: Genome scale engineering techniques for metabolic engineering.
abstract: Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization, and a synthesis step that fuels additional hypothesis generation. This cycle mirrors the Design-Build-Test-Learn cycle followed throughout various engineering fields that has recently become a defining aspect of synthetic biology. This review will attempt to summarize recent genome-scale design, build, test, and learn technologies and relate their use to a range of metabolic engineering applications.
authors.affiliation: Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80309, United States. Electronic address: rongming.liu@colorado.edu.
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, United States. Electronic address: marcelo.bassalo@colorado.edu.
authors.forename: Marcelo C
authors.initials: MC
authors.lastname: Bassalo
authors.affiliation: Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80309, United States. Electronic address: ramsey.zeitoun@colorado.edu.
authors.forename: Ramsey I
authors.initials: RI
authors.lastname: Zeitoun
authors.affiliation: Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80309, United States. Electronic address: rtg@colorado.edu.
authors.forename: Ryan T
authors.initials: RT
authors.lastname: Gill
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D016678:Genome / Q000235:genetics*; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods*; D058615:Synthetic Biology / Q000639:trends*
chemical_list: D004247:DNA
keywords: Design-Build-Test-Learn; Genome scale engineering; Metabolic engineering; Synthetic biology