title: Jasmonate signalling drives time-of-day differences in susceptibility of Arabidopsis to the fungal pathogen Botrytis cinerea.
abstract: The circadian clock, an internal time-keeping mechanism, allows plants to anticipate regular changes in the environment, such as light and dark, and biotic challenges such as pathogens and herbivores. Here, we demonstrate that the plant circadian clock influences susceptibility to the necrotrophic fungal pathogen, Botrytis cinerea. Arabidopsis plants show differential susceptibility to B. cinerea depending on the time of day of inoculation. Decreased susceptibility after inoculation at dawn compared with night persists under constant light conditions and is disrupted in dysfunctional clock mutants, demonstrating the role of the plant clock in driving time-of-day susceptibility to B. cinerea. The decreased susceptibility to B. cinerea following inoculation at subjective dawn was associated with faster transcriptional reprogramming of the defence response with gating of infection-responsive genes apparent. Direct target genes of core clock regulators were enriched among the transcription factors that responded more rapidly to infection at subjective dawn than subjective night, suggesting an influence of the clock on the defence-signalling network. In addition, jasmonate signalling plays a crucial role in the rhythmic susceptibility of Arabidopsis to B. cinerea with the enhanced susceptibility to this pathogen at subjective night lost in a jaz6 mutant.
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Ingle
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Stoker
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Wendy
authors.initials: W
authors.lastname: Stone
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Nicolette
authors.initials: N
authors.lastname: Adams
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Rob
authors.initials: R
authors.lastname: Smith
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, EX4 4QD, UK.
authors.forename: Murray
authors.initials: M
authors.lastname: Grant
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Carré
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, 7701, South Africa.
authors.forename: Laura C
authors.initials: LC
authors.lastname: Roden
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.|Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Denby
mesh_terms: D017360:Arabidopsis / Q000378:metabolism / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000235:genetics; D020171:Botrytis / Q000472:pathogenicity*; D057906:Circadian Clocks; D003517:Cyclopentanes / Q000378:metabolism*; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C554742:JAZ6 protein, Arabidopsis; D054883:Oxylipins; D012097:Repressor Proteins; C011006:jasmonic acid
keywords: Arabidopsis thaliana; Botrytis cinerea; circadian clock; defence gene expression; defence response; disease resistance; jasmonate; plant-pathogen interaction