title: MicroRNA-128b suppresses tumor growth and promotes apoptosis by targeting A2bR in gastric cancer.
abstract: MicroRNAs (miRNAs) play crucial roles in the development and progression of human cancers, including gastric cancer (GC). The discovery of miRNAs may provide a new and powerful tool for studying the mechanism, diagnosis, and treatment of GC. In this study, we aimed to investigate the role and mechanism of miR-128b in the development and progression of GC. Quantitative real-time PCR (qRT-PCR) was used to measure the expression level of miR-128b in GC tissues and cell lines. We found that miR-128b was significantly down-regulated in GC tissues and cell lines. In addition, over-expression of miR-128b inhibited GC cell proliferation, migration and invasion of GC cells in vitro. Gain-of-function in vitro experiments further showed that the miR-128b mimic significantly promoted GC cell apoptosis. Subsequent dual-luciferase reporter assay identified one of the proto-oncogene A2bR as direct target of miR-128b. Therefore, our results indicate that miR-128b is a proto-oncogene miRNA that can suppresses GC proliferation and migration through down-regulation of the oncogene gene A2bR. Taken together, our results indicate that miR-128b could serve as a potential diagnostic biomarker and therapeutic option for human GC in the near future.
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710068, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710068, China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710068, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zong
authors.affiliation: Department of General Surgery, The Third Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710068, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710068, China.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: fisrstsxianghe@163.com.
authors.forename: Shuixiang
authors.initials: S
authors.lastname: He
mesh_terms: D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics; D043704:Receptor, Adenosine A2B / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C000715470:MAS1 protein, human; C545189:MIRN128 microRNA, human; D035683:MicroRNAs; D000090063:Proto-Oncogene Mas; D043704:Receptor, Adenosine A2B
keywords: A2bR; Apoptosis; Gastric cancer; Proliferation; miR-128b