title: Neuroprotective effect of methylprednisolone combined with placenta-derived mesenchymal stem cell in rabbit model of spinal cord injury.
abstract: The aim of this study was to assess the ability of the combination treatment of methylprednisolone (MP) and placenta-derived mesenchymal stem cells (PDMSCs) in a rabbit model of spinal cord injury (SCI). Rabbits were randomly divided into four groups: group 1 (control), group 2 (MP), group 3 (PDMSCs) and group 4 (MP + PDMSCs). In all groups, the spinal cord injury model was created by the weight drop method. Levels of malondialdehyde (MDA), myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined by kit. Histopathological examination was also performed. Neurological evaluation was carried out with the Tarlov scoring system. The results showed both MP and PDMSCs had neuroprotective effects, and combining the administration of MP with PDMSCs was shown a significant effect on the recovery of neurological function. Therefore, the combined use of MP and PDMSCs can be used as a potential therapeutic method for SCI.
authors.affiliation: Department of Spine Surgery, Yantai Affiliated Hospital of Binzhou Medical University Yantai 264100, China.
authors.forename: Jiang-Wei
authors.initials: JW
authors.lastname: Tan
authors.affiliation: Department of Anesthesiology, Yantaishan Hospital Yantai 264000, China.
authors.forename: Kai-Yan
authors.initials: KY
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Oncology, Yantaishan Hospital Yantai 264000, China.
authors.forename: Guang-Jun
authors.initials: GJ
authors.lastname: Liao
authors.affiliation: Department of Spine Surgery, Yantai Affiliated Hospital of Binzhou Medical University Yantai 264100, China.
authors.forename: Fang-Min
authors.initials: FM
authors.lastname: Chen
authors.affiliation: Department of Pediatric Orthopedics, Yantaishan Hospital Yantai 264000, China.
authors.forename: Ming-Zhang
authors.initials: MZ
authors.lastname: Mu
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D008775:Methylprednisolone / Q000627:therapeutic use*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011817:Rabbits; D020127:Recovery of Function / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D018696:Neuroprotective Agents; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008775:Methylprednisolone
keywords: Neuroprotective; methylprednisolone; placenta-derived mesenchymal stem cell; spinal cord injury