title: Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.
abstract: Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae as a model organism, we developed a system that recapitulates and arrests ribosome assembly at early stages, thus providing in vivo snapshots of nascent preribosomal particles. Here we report the stage-specific order in which 70 ribosome-assembly factors associate with preribosomal RNA domains, thereby forming the 6-MDa small-subunit processome.
authors.affiliation: Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, New York, USA.|Tri-Institutional Training Program in Chemical Biology, Rockefeller University, New York, New York, USA.
authors.forename: Malik
authors.initials: M
authors.lastname: Chaker-Margot
authors.affiliation: Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, New York, USA.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Hunziker
authors.affiliation: Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, New York, USA.
authors.forename: Jonas
authors.initials: J
authors.lastname: Barandun
authors.affiliation: Proteomics Resource Center, Rockefeller University, New York, New York, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Dill
authors.affiliation: Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, New York, USA.
authors.forename: Sebastian
authors.identifier: http://orcid.org/0000-0002-9373-4737
authors.initials: S
authors.lastname: Klinge
mesh_terms: D046911:Macromolecular Substances / Q000378:metabolism; D008954:Models, Biological; D001678:Organelle Biogenesis; D012335:RNA, Ribosomal / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*
chemical_list: D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins