title: Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses.
abstract: Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
authors.affiliation: Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. vgtran@wisc.edu.|Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA. vgtran@wisc.edu.
authors.forename: Vy
authors.initials: V
authors.lastname: Tran
authors.affiliation: Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. dspoole@wisc.edu.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Poole
authors.affiliation: Carbone Cancer Center, University of Wisconsin Madison, Madison, WI 53706, USA. jjjeffery@wisc.edu.
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Jeffery
authors.affiliation: GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. sheahan@email.unc.edu.
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Sheahan
authors.affiliation: GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. donald.r.creech@gsk.com.
authors.forename: Donald
authors.initials: D
authors.lastname: Creech
authors.affiliation: PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. Aleksey.Yevtodiyenko@perkinelmer.com.
authors.forename: Aleksey
authors.initials: A
authors.lastname: Yevtodiyenko
authors.affiliation: GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. andy.j.peat@gsk.com.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Peat
authors.affiliation: PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. Kevin.Francis@perkinelmer.com.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Francis
authors.affiliation: GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. shihyun.k.you@gsk.com.
authors.forename: Shihyun
authors.initials: S
authors.lastname: You
authors.affiliation: Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. amehle@wisc.edu.
authors.forename: Andrew
authors.initials: A
authors.lastname: Mehle
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D009980:Influenza A virus / Q000254:growth & development*; D008163:Luminescent Measurements / Q000379:methods; D008807:Mice, Inbred BALB C; D064847:Multimodal Imaging / Q000379:methods*; D061848:Optical Imaging / Q000379:methods; D009976:Orthomyxoviridae Infections / Q000188:drug therapy / Q000473:pathology* / Q000821:virology*; D013194:Staining and Labeling / Q000379:methods; D016896:Treatment Outcome; D019562:Viral Load; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D000998:Antiviral Agents
keywords: influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus