title: Osteogenesis of human adipose-derived stem cells on poly(dopamine)-coated electrospun poly(lactic acid) fiber mats.
abstract: Electrospinning is a versatile technique to generate large quantities of micro- or nano-fibers from a wide variety of shapes and sizes of polymer. The aim of this study is to develop functionalized electrospun nano-fibers and use a mussel-inspired surface coating to regulate adhesion, proliferation and differentiation of human adipose-derived stem cells (hADSCs). We prepared poly(lactic acid) (PLA) fibers coated with polydopamine (PDA). The morphology, chemical composition, and surface properties of PDA/PLA were characterized by SEM and XPS. PDA/PLA modulated hADSCs' responses in several ways. Firstly, adhesion and proliferation of hADSCs cultured on PDA/PLA were significantly enhanced relative to those on PLA. Increased focal adhesion kinase (FAK) and collagen I levels and enhanced cell attachment and cell cycle progression were observed upon an increase in PDA content. In addition, the ALP activity and osteocalcin of hADSCs cultured on PDA/PLA were significantly higher than seen in those cultured on a pure PLA mat. Moreover, hADSCs cultured on PDA/PLA showed up-regulation of the ang-1 and vWF proteins associated with angiogenesis differentiation. Our results demonstrate that the bio-inspired coating synthetic degradable PLA polymer can be used as a simple technique to render the surfaces of synthetic biodegradable fibers, thus enabling them to direct the specific responses of hADSCs.
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan. Electronic address: chichang31@thu.edu.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan.
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Fu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D042683:Angiopoietin-1 / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D042683:Angiopoietin-1; D000900:Anti-Bacterial Agents; D007211:Indoles; D011091:Polyesters; D011108:Polymers; C568283:polydopamine; D014841:von Willebrand Factor; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: Angiogenic; Dopamine; Nanofiber; Osteogenic; Poly(lactic acid); Tissue engineering