title: Biological interaction of thiamine with lysozyme using binding capacity concept and molecular docking.
abstract: The binding of thiamine (vitamin B1) on lysozyme has been examined at various ionic strengths of phosphate buffer (pH 6.9), various pH values, and various protein concentrations at 25°C using thiamine selective membrane electrode. This method is faster and more precise than equilibrium dialysis technique which can obtain sufficient and accurate data for binding analysis. The values of Hill equation parameters were estimated for each set using binding capacity concept and used for calculation of intrinsic binding affinity. The results represent two binding sets for thiamine on lysozyme at various experimental conditions.
authors.affiliation: a Medical Laser Research Center , ACECR , Tehran , Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Hosseinzadeh
authors.affiliation: a Medical Laser Research Center , ACECR , Tehran , Iran.
authors.forename: Khatereh
authors.initials: K
authors.lastname: Khorsandi
authors.affiliation: b Institute of Biochemistry and Biophysics , University of Tehran , Tehran , Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Sheikh-Hasani
authors.affiliation: b Institute of Biochemistry and Biophysics , University of Tehran , Tehran , Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khatibi
mesh_terms: D001665:Binding Sites; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013329:Structure-Activity Relationship; D013831:Thiamine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D008024:Ligands; D009113:Muramidase; D013831:Thiamine
keywords: binding capacity; drug selective electrode; lysozyme; molecular docking; thiamine