title: Enhancing acetone biosynthesis and acetone-butanol-ethanol fermentation performance by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae integrated with exogenous acetate addition.
abstract: Acetone is the major by-product in ABE fermentations, most researches focused on increasing butanol/acetone ratio by decreasing acetone biosynthesis. However, economics of ABE fermentation industry strongly relies on evaluating acetone as a valuable platform chemical. Therefore, a novel ABE fermentation strategy focusing on bio-acetone production by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae with exogenous acetate addition was proposed. Experimental and theoretical analysis revealed the strategy could, enhance C. acetobutylicum survival oriented amino acids assimilation in the cells; control NADH regeneration rate at moderately lower level to enhance acetone synthesis but without sacrificing butanol production; enhance the utilization ability of C. acetobutylicum on glucose and direct most of extra consumed glucose into acetone/butanol synthesis routes. By implementing the strategy using synthetic or acetate fermentative supernatant, acetone concentrations increased to 8.27-8.55g/L from 5.86g/L of the control, while butanol concentrations also elevated to the higher levels of 13.91-14.23g/L from 11.63g/L simultaneously.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Luo
authors.affiliation: China Shijiazhuang Pharmaceutical Group Co., Ltd., Shijiazhuang 050038, China.
authors.forename: Laibing
authors.initials: L
authors.lastname: Ge
authors.affiliation: China Shijiazhuang Pharmaceutical Group Co., Ltd., Shijiazhuang 050038, China.
authors.forename: Jingshu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: zpshi@jiangnan.edu.cn.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Shi
mesh_terms: D020001:1-Butanol / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000096:Acetone / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D018920:Coculture Techniques; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D009243:NAD / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000085:Acetates; D009243:NAD; D000096:Acetone; D000431:Ethanol; D020001:1-Butanol
keywords: ABE fermentation; Acetate; Acetone bio-synthesis; Amino acids; NADH