title: Rapid detection of Cu(2+) by a paper-based microfluidic device coated with bovine serum albumin (BSA)-Au nanoclusters.
abstract: In this work, bovine serum albumin (BSA)-Au nanoclusters were used to coat a paper-based microfluidic device. This device acted as a Cu(2+) biosensor that showed fluorescence quenching on detection of copper ions. The detection limit of this sensor could be adjusted by altering the water absorbing capacity of the device. Qualitative and semi-quantitative results could be obtained visually without the aid of any advanced instruments. This sensor could test Cu(2+) rapidly with high specificity and sensitivity, which would be useful for point-of-care testing (POCT).
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University; Shanghai Suxin Biotech. Co., Ltd, Shanghai, P.R. China. jlkong@fudan.edu.cn and Shanghai Suxin Biotech. Co., Ltd, Shanghai, 200443, P.R. China.
authors.forename: Xueen
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China. jlkong@fudan.edu.cn.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China. jlkong@fudan.edu.cn.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China. jlkong@fudan.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China. jlkong@fudan.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
authors.affiliation: Department of Chemistry and Department of laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, P.R. China. jlkong@fudan.edu.cn.
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002413:Cations, Divalent / Q000032:analysis; D002417:Cattle; D003300:Copper / Q000032:analysis*; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010209:Paper; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D002413:Cations, Divalent; D012710:Serum Albumin, Bovine; D006046:Gold; D003300:Copper