title: Lack of Cytosolic Carboxypeptidase 1 Leads to Subfertility due to the Reduced Number of Antral Follicles in pcd3J-/- Females.
abstract: Females homozygous for the Purkinje cell degeneration mutation (pcd) are fertile, although the success rate is much lower than in the wild type. We performed detailed analysis of reproductive abnormalities of pcd females. The number of oocytes produced following exogenous gonadotropin treatment was much lower in pcd3J-/- females than in pcd3J+/+ females. Furthermore, the estrous cyclicity of pcd3J-/- females according to the appearance of the vagina was almost undetectable comparing to that of the wild type. Histological analyses and follicle counting of 4- and 8-week-old pcd3J-/- ovaries showed an increase in the number of secondary follicles and a decrease in the number of antral follicles, indicating that AGTPBP1/ CCP1 plays an important role in the development of secondary follicles into antral follicles. Consistent with a previous analysis of the pcd cerebellum, pcd3J-/- ovaries also showed a clear increase in the level of polyglutamylation. Gene expression analysis showed that both oocytes and cumulus cells express CCP1. However, Ccp4 and CCP6, which can compensate the function of CCP1, were not expressed in mouse ovaries. Failure of microtubule deglutamylation did not affect the structure and function of the meiotic spindle in properly aligning chromosomes in the center of the nucleus during meiosis in pcd3J-/- females. We also showed that the pituitary-derived growth and reproduction-related endocrine system functions normally in pcd3J-/- mice. The results of this study provide insight into additional functions of CCP1, which cannot be fully explained by the side chain deglutamylation of microtubules alone.
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Ning
authors.initials: N
authors.lastname: Song
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Nameun
authors.initials: N
authors.lastname: Kim
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Hojun
authors.initials: H
authors.lastname: Choi
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Hyo-Im
authors.initials: HI
authors.lastname: Chun
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Min-Hee
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Colleges of Veterinary Medicine, Konkuk University, Seoul, South Korea.
authors.forename: Kunho
authors.initials: K
authors.lastname: Seo
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Nagasundarapandian
authors.initials: N
authors.lastname: Soundrarajan
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Jeong-Tae
authors.initials: JT
authors.lastname: Do
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.affiliation: College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, P.R. China.
authors.forename: Zhao-Jia
authors.initials: ZJ
authors.lastname: Ge
authors.affiliation: Department of Animal Biotechnology, Konkuk University, Seoul, South Korea.
authors.forename: Chankyu
authors.initials: C
authors.lastname: Park
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics / Q000378:metabolism; D054885:Cumulus Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005298:Fertility; D019204:GTP-Binding Proteins / Q000172:deficiency*; D008540:Meiosis; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes / Q000378:metabolism* / Q000473:pathology; D011689:Purkinje Cells / Q000378:metabolism / Q000473:pathology; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase / Q000172:deficiency*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism
chemical_list: D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase; C420042:Agtpbp1 protein, mouse; D019204:GTP-Binding Proteins