title: Studying the Translatome with Polysome Profiling.
abstract: Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Via delle Regole 101, 38123, Mattarello, Trento, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Zuccotti
authors.affiliation: Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, Via delle Regole 101, 38123, Mattarello, Trento, Italy. angelika.modelska@unitn.it.
authors.forename: Angelika
authors.initials: A
authors.lastname: Modelska
mesh_terms: D016678:Genome; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D011132:Polyribosomes / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011506:Proteins; D012333:RNA, Messenger
keywords: Polysome; Profiling; Protein isolation; RNA isolation; Sucrose gradient; Transcriptome; Translational control; Translatome