title: The change of microbial community from chlorinated solvent-contaminated groundwater after biostimulation using the metagenome analysis.
abstract: The compositions of bacterial community in one site contaminated with PCE/TCE after the slow polycolloid-releasing substrate (SPRS) (contained vegetable oil, cane molasses, and surfactants) addition were analyzed. Results show that SPRS caused a rapid enhancement of reductive dechlorination of TCE. The transformation of PCE/TCE into ethene was observed after 20 days of operation. To compare the change of bacterial communities before and after SPRS addition, 16S rRNA amplicon sequencing using the metagenome analysis was performed. Results demonstrated the detection of the increased amounts of Dehalogenimonas by 2.2-fold, Pseudomonas by 3.4-fold and Sulfuricurvum by 4-fold with the analysis of the ribosomal database project (RDP). Metagenomic DNA was extracted from PCE/TCE-contaminated groundwater after SPRS addition, and subjected to sequencing. Results obtained from metagenomic sequencing indicate that genes from Dehalococcoides mccartyi was ranked as the second abundant bacteria among all of the detected bacteria via the analysis of the lowest common ancestor (LCA). Abundance of these bacterial groups, as shown above suggests their role in TCE biodegradation. Functional analysis of the metagenome, with the specific reference to chloroalkane and chloroalkene degradation, revealed the presence of some genes responsible for TCE biodegradation. Overall, results of this study provided new insights for a better understanding of the potential of biostimulation on TCE-contaminated sites.
authors.affiliation: Institute of Environmental Engineering, National Sun Yat-Sen University, no. 70, Lienhai Rd., Kaohsiung 80424, Taiwan.
authors.forename: Chih-Ming
authors.initials: CM
authors.lastname: Kao
authors.affiliation: Department of Life Sciences, National Central University, no. 300, Jhingda Rd., Jhongli City, Taoyuan 32001, Taiwan.
authors.forename: Hung-Yu
authors.initials: HY
authors.lastname: Liao
authors.affiliation: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, no. 135, Yuantung Rd., Jhongli City, Taoyuan 32003, Taiwan.
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Chien
authors.affiliation: Graduate Institute of Statistics, National Central University, no. 300, Jhingda Rd., Jhongli City, Taoyuan 32001, Taiwan.
authors.forename: Yi-Kuan
authors.initials: YK
authors.lastname: Tseng
authors.affiliation: Department of Parasitology, Chang Gung University, no.259, Wenhua 1st Rd., Guishan Dis., Taoyuan City 33302, Taiwan.
authors.forename: Petrus
authors.initials: P
authors.lastname: Tang
authors.affiliation: Jeptro Technology Co., Ltd., no. 211, 23F-1, Jhongjheng 4th Rd., Kaohsiung 801, Taiwan.
authors.forename: Chih-En
authors.initials: CE
authors.lastname: Lin
authors.affiliation: Department of Life Sciences, National Central University, no. 300, Jhingda Rd., Jhongli City, Taoyuan 32001, Taiwan. Electronic address: osycchna@ksts.seed.net.tw.
authors.forename: Ssu-Ching
authors.initials: SC
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D060587:Groundwater / Q000382:microbiology*; D056186:Metagenomics; D064307:Microbiota / Q000187:drug effects*; D014241:Trichloroethylene / Q000633:toxicity*
chemical_list: D014241:Trichloroethylene
keywords: Dechlorination; Metagenome; Microbial community; Tetrachloroethane; Trichloroethene