title: Green synthesis of silver nanoparticles using Coffea arabica seed extract and its antibacterial activity.
abstract: A novel green source was opted to synthesize silver nanoparticles using dried roasted Coffea arabica seed extract. Bio-reduction of silver was complete when the mixture (AgNO3+extract) changed its color from light to dark brown. UV-vis spectroscopy result showed maximum adsorption at 459 nm, which represents the characteristic surface plasmon resonance of nanosilver. X-ray crystal analysis showed that the silver nanoparticles are highly crystalline and exhibit a cubic, face centered lattice with characteristic (111), (200), (220) and (311) orientations. Particles exhibit spherical and ellipsoidal shaped structures as observed from TEM. Composition analysis obtained from SEM-EDXA confirmed the presence of elemental signature of silver. FTIR results recorded a downward shift of absorption bands between 800-1500 cm(-1) indicting the formation of silver nanoparticles. The mean particle size investigated using DLS was found to be in between 20-30 nm respectively. Anti-bacterial activity of silver nanoparticles on E. coli and S. aureus demonstrated diminished bacterial growth with the development of well-defined inhibition zones.
authors.affiliation: Center for Nanoscience and Technology, IST, JNTUH, Kukatpally, Hyderabad, 500085 AP, India; Centre for Knowledge Management of Nanoscience and Technology, 12-5-32/8, Vijayapuri Colony, Tarnaka, 500017 AP, India; Department of Mechanical Engineering, College of Engineering, Kyung Hee University, 446-701 Yongin, Republic of Korea. Electronic address: vivekdhand2012@gmail.com.
authors.forename: Vivek
authors.initials: V
authors.lastname: Dhand
authors.affiliation: Center for Nanoscience and Technology, IST, JNTUH, Kukatpally, Hyderabad, 500085 AP, India.
authors.forename: L
authors.initials: L
authors.lastname: Soumya
authors.affiliation: Department of Physics, Gitam Institute of Technology, Gitam University, Vishakapatnam, 530045 Andhra Pradesh, India.
authors.forename: S
authors.initials: S
authors.lastname: Bharadwaj
authors.affiliation: Center for Nanoscience and Technology, IST, JNTUH, Kukatpally, Hyderabad, 500085 AP, India.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Chakra
authors.affiliation: Centre for Knowledge Management of Nanoscience and Technology, 12-5-32/8, Vijayapuri Colony, Tarnaka, 500017 AP, India. Electronic address: deepikabhatt@gmail.com.
authors.forename: Deepika
authors.initials: D
authors.lastname: Bhatt
authors.affiliation: I &PC Division, IICT, Tarnaka, Hyderabad 500007, India.
authors.forename: B
authors.initials: B
authors.lastname: Sreedhar
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D040503:Coffea / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver
keywords: Antibacterial activity; Coffee; Seed extract; Silver nanoparticles; UV–vis spectroscopy