title: Wheat TaSP gene improves salt tolerance in transgenic Arabidopsis thaliana.
abstract: A novel salt-induced gene with unknown functions was cloned through analysis of gene expression profile of a salt-tolerant wheat mutant RH8706-49 under salt stress. The gene was named Triticum aestivum salt-related protein (TaSP) and deposited in GenBank (Accession No. KF307326). Quantitative polymerase chain reaction (qPCR) results showed that TaSP expression was induced under salt, abscisic acid (ABA), and polyethylene glycol (PEG) stresses. Subcellular localization revealed that TaSP was mainly localized in cell membrane. Overexpression of TaSP in Arabidopsis could improve salt tolerance of 35S::TaSP transgenic Arabidopsis. 35S::TaSP transgenic Arabidopsis lines after salt stress presented better physiological indexes than the control group. In the non-invasive micro-test (NMT), an evident Na(+) excretion was observed at the root tip of salt-stressed 35S::TaSP transgenic Arabidopsis. TaSP promoter was cloned, and its beta-glucuronidase (GUS) activities before and after ABA, salt, cold, heat, and salicylic acid (SA) stresses were determined. Full-length TaSP promoter contained ABA and salt response elements.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China. Electronic address: maxiaoli04@163.com.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China. Electronic address: cuiweina102@163.com.
authors.forename: Weina
authors.initials: W
authors.lastname: Cui
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China; College of Clinical Medicine, North China University of Science and Technology, Tangshan 063000, People's Republic of China. Electronic address: liangwenji_2005@163.com.
authors.forename: Wenji
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China. Electronic address: huangzhanjing@mail.hebtu.edu.cn.
authors.forename: Zhanjing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011092:Polyethylene Glycols / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D055049:Salt Tolerance; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014908:Triticum / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000040:Abscisic Acid
keywords: GUS dyeing; NMT; Salt tolerance; Wheat; qPCR