title: Bacillus depressus sp. nov., isolated from soil of a sunflower field.
abstract: A Gram-stain positive, rod-shaped, endospore-forming and aerobic bacterium, designated BZ1(T), was isolated from a soil sample collected from a sunflower field in Wuyuan county, Inner Mongolia, China. On the basis of 16S rRNA gene sequence analysis, the isolate was found to be a member of the genus Bacillus and the close phylogenetic relatives to be Bacillus gottheilii WCC 4585(T), Bacillus oceanisediminis H2(T), Bacillus mesonae FJAT-13985(T) and Bacillus horneckiae DSM 23495(T) with 98.3, 98.1, 98.0 and 97.6 % sequence similarity, respectively. Strain BZ1(T) was found to grow at 6-40 °C (optimum 30-33 °C), pH 6.0-9.0 (optimum pH 7.0) and 0-5.5 % (w/v) NaCl (optimum 0.5 %). The cell wall diamino acid of the peptidoglycan of strain BZ1(T) was identified as meso-diaminopimelic acid and the predominant respiratory quinone as MK-7. The major cellular fatty acids were found to be iso-C15:0, anteiso-C15:0 and iso-C14:0, and the polar lipids to consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The novel strain was found to have a DNA G + C content 44.5 mol%. DNA-DNA hybridization with closely related strains was low. Based on phenotypic, phylogenetic and chemotaxonomic results, it is concluded that strain BZ1(T) represents a novel species within the genus Bacillus, for which we propose the name Bacillus depressus sp. nov. The type strain is BZ1(T) (= CGMCC 1.15124(T) = KCTC 33643(T)).
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Xuexin
authors.initials: X
authors.lastname: Wei
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Xin
authors.affiliation: China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xin
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Tianying
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China. zhangjianli@bit.edu.cn.
authors.forename: Jianli
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016227:Benzoquinones / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000737:chemistry; D020404:Glycerophospholipids / Q000737:chemistry; D006368:Helianthus / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D016227:Benzoquinones; D004269:DNA, Bacterial; D005227:Fatty Acids; D020404:Glycerophospholipids; D010457:Peptidoglycan; C004532:quinone; D003960:Diaminopimelic Acid
keywords: 16S rRNA; Bacillus depressus sp. nov; Polyphasic taxonomy; Soil