title: Detection of Epstein-Barr virus in different sources of materials from patients with oral lichen planus: a case-control study.
abstract: OBJECTIVE
  To detect the presence of Epstein-Barr virus (EBV) DNA in different sources of materials from a matched group of patients with oral lichen planus (OLP) and a group of people without OLP lesions, and to correlate the presence of virus with epidemiological variables of the groups studied.
  
  
  METHODS
  Fresh tissue samples, saliva, exfoliated cells and plasma of 24 patients with OLP lesions (cases) and 17 patients without OLP lesions (controls) were collected. EBV was detected by nested PCR.
  
  
  RESULTS
  Viral positivity was obtained in 62.5% of tissue samples; in 70.8% of exfoliated cell samples; in 33.3% of blood plasma samples and in 75% of saliva samples in the cases; and in 35.3% of tissue samples; 82.4% of exfoliated cell samples; in 47.1% of blood plasma samples and in 64.7% of saliva samples in the controls. There was a predominance of women in both groups. Variables not atrophic-erosive were most affected by EBV.
  
  
  CONCLUSIONS
  No relationship between EBV and OLP was found. However, all sources tested in this study were considered suitable for the detection of viruses.
authors.affiliation: Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
authors.forename: Rúbia da Rocha
authors.initials: Rda R
authors.lastname: Vieira
authors.affiliation: Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
authors.forename: Lígia Lavezo
authors.identifier: http://orcid.org/0000-0002-2871-9346
authors.initials: LL
authors.lastname: Ferreira
authors.affiliation: Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
authors.forename: Éder Ricardo
authors.initials: ÉR
authors.lastname: Biasoli
authors.affiliation: Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
authors.forename: Daniel Galera
authors.initials: DG
authors.lastname: Bernabé
authors.affiliation: Animal Health and Production, Araçatuba School of Veterinary Medicine, Araçatuba, São Paulo, Brazil.
authors.forename: Cáris Maroni
authors.initials: CM
authors.lastname: Nunes
authors.affiliation: Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, Araçatuba, São Paulo, Brazil.
authors.forename: Glauco Issamu
authors.initials: GI
authors.lastname: Miyahara
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology*; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D017676:Lichen Planus, Oral / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D004279:DNA, Viral
keywords: EBV; MOLECULAR BIOLOGY; ORAL PATHOLOGY