title: EphB3 receptors function as dependence receptors to mediate oligodendrocyte cell death following contusive spinal cord injury.
abstract: We demonstrate that EphB3 receptors mediate oligodendrocyte (OL) cell death in the injured spinal cord through dependence receptor mechanism. OLs in the adult spinal cord express EphB3 as well as other members of the Eph receptor family. Spinal cord injury (SCI) is associated with tissue damage, cellular loss and disturbances in EphB3-ephrinB3 protein balance acutely (days) after the initial impact creating an environment for a dependence receptor-mediated cell death to occur. Genetic ablation of EphB3 promotes OL survival associated with increased expression of myelin basic protein and improved locomotor function in mice after SCI. Moreover, administration of its ephrinB3 ligand to the spinal cord after injury also promotes OL survival. Our in vivo findings are supported by in vitro studies showing that ephrinB3 administration promotes the survival of both oligodendroglial progenitor cells and mature OLs cultured under pro-apoptotic conditions. In conclusion, the present study demonstrates a novel dependence receptor role of EphB3 in OL cell death after SCI, and supports further development of ephrinB3-based therapies to promote recovery.
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsenkina
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.|Department of Biology, Drexel University, Philadelphia, PA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ricard
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.
authors.forename: E
authors.initials: E
authors.lastname: Runko
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Quiala-Acosta
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mier
authors.affiliation: The Miami Project to Cure Paralysis, The Department of Neurosurgery, Miller School of Medicine, The University of Miami, Miami, FL, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Liebl
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D036389:Ephrin-B3 / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D018345:Mice, Knockout; D009836:Oligodendroglia / Q000502:physiology*; D036223:Receptor, EphB3 / Q000502:physiology*; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology
chemical_list: D036389:Ephrin-B3; D036223:Receptor, EphB3