title: Reducing the infectivity and richness of ectomycorrhizal fungi in a calcareous Quercus ilex forest through soil preparations for truffle plantation establishment: A bioassay study.
abstract: In the early years of a black truffle plantation, the field proliferation of the nursery-inoculated fungi can be hampered by native ectomycorrhizal fungi colonising the seedling roots. Reducing the soil ectomycorrhizal infectivity in the planting hole before introducing the inoculated seedling could be an effective strategy to reduce this problem. Three bioassays were conducted to evaluate the impact of several soil preparations on the ectomycorrhizal infectivity and richness of a Quercus ilex soil in a truffle-producing region. Microwaves, quicklime, and acetic acid significantly decreased the percent root colonisation and morphotype richness of the native ectomycorrhizal fungi. However, they also decreased seedling survival or growth. Peracetic acid, hydrogen peroxide, and sodium hypochlorite did not show a significant negative effect on the soil ectomycorrhizal community. The results support the potential of soil preparation for reducing the ectomycorrhizal infectivity of forest soils, thus being a promising strategy to reduce the early colonisation by native fungi in truffle plantations. However, the indications of damage to the seedling development must be addressed.
authors.affiliation: Fundación Centro de Estudios Ambientales del Mediterráneo (CEAM), C/Charles Darwin 14, Parque Tecnológico, 46980 Paterna, Valencia, Spain. Electronic address: sergigarciabarreda@gmail.com.
authors.forename: Sergi
authors.initials: S
authors.lastname: Garcia-Barreda
authors.affiliation: Fundación Centro de Estudios Ambientales del Mediterráneo (CEAM), C/Charles Darwin 14, Parque Tecnológico, 46980 Paterna, Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Molina-Grau
authors.affiliation: ETS Ingeniería Agronómica y del Medio Natural, Universitat Politècnica de València, Camino de Vera s/n, 46021 Valencia, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Reyna
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D001203:Ascomycota / Q000254:growth & development*; D017610:Calcium Compounds / Q000633:toxicity; D004203:Disinfection / Q000379:methods*; D065928:Forests; D008872:Microwaves; D038821:Mycorrhizae / Q000187:drug effects* / Q000528:radiation effects*; D010087:Oxides / Q000633:toxicity; D063245:Plant Development / Q000187:drug effects / Q000528:radiation effects; D018517:Plant Roots / Q000382:microbiology*; D029963:Quercus / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology* / Q000528:radiation effects; D012988:Soil Microbiology
chemical_list: D017610:Calcium Compounds; D010087:Oxides; C016538:lime; D019342:Acetic Acid
keywords: Acetic acid; Ectomycorrhizal infectivity; Liming; Microwaves