title: Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel.
abstract: Dipeptides and their derivatives have attracted tremendous attention owning to their excellent abilities of self-assemble assembling into various structures which have great potentials for applications in biology and/or nanotechnology. In the present study, we dedicate to fabricate a rigid and structure controllable Fmoc-FF/SA composite hydrogel. We found that the modified dipeptide, fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) can self-assemble into rigid hydrogels with structures of nanowires, layered thin films or honeycombs as the change of sodium alginate (SA) concentration. Meanwhile, CD-spectroscopy demonstrated that SA appeared to control the process, but it did not change the arrangement of the Fmoc-FF peptide. Our results demonstrated that the formed hydrogel showed physical and chemical stability as well as possessing good biocompatibility. Rheological measurements showed that the addition of SA could improve the stability of the hydrogel. Cell viability assay revealed that the Fmoc-FF and Fmoc-FF/SA hydrogels are both beneficial for cell proliferation in-vitro. Our results indicated that the fabricated Fmoc-FF/SA composite hydrogels could be used in tissue engineering and drug delivery in the future.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gong
authors.affiliation: Institute for Health Research and Policy, London Metropolitan University, London N78 DB, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Branford-White
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: Changzhou Institute of Engineering Technology, Changzhou 213164, PR China.
authors.forename: Shubai
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Quan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, PR China. Electronic address: niehuali@dhu.edu.cn.
authors.forename: Huali
authors.initials: H
authors.lastname: Nie
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: lzhu@dhu.edu.cn.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000464:Alginates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004151:Dipeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005449:Fluorenes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012212:Rheology / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D013818:Thermogravimetry
chemical_list: D000464:Alginates; D004151:Dipeptides; D005449:Fluorenes; D006603:Hexuronic Acids; C000609769:fluorenyl-9-methoxycarbonyl diphenylalanine; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid
keywords: Hydrogel; Peptide; Self-assembly; Sodium alginate; Structure controllable