title: Reduced expression of exocytotic proteins caused by anti-cholinesterase pesticides in Brachionus calyciflorus (Rotifera: Monogononta).
abstract: The organophosphate and carbamate pesticides methyl-parathion and carbaryl have a common action mechanism: they inhibit acetylcholinesterase enzyme by blocking the transmission of nerve impulses. However, they can alter the expression of exocytotic membrane proteins (SNARE), by modifying release of neurotransmitters and other substances. This study evaluated the adverse effects of the pesticides methyl-parathion and carbaryl on expression of SNARE proteins: Syntaxin-1, Syntaxin-4 and SNAP-23 in freshwater rotifer Brachionus calyciflorus. Protein expression of these three proteins was analyzed before and after exposure to these two pesticides by Western Blot. The expression of Syntaxin-1, Syntaxin-4 and SNAP-23 proteins in B. calyciflorussignificantly decreases with increasing concentration of either pesticides. This suggests that organophosphates and carbamates have adverse effects on expression of membrane proteins of exocytosis by altering the recognition, docking and fusion of presynaptic and vesicular membranes involved in exocytosis of neurotransmitters. Our results demonstrate that the neurotoxic effect of anticholinesterase pesticides influences the interaction of syntaxins and SNAP-25 and the proper assembly of the SNARE complex.
authors.affiliation: División de Estudios de Posgrado e Investigación, Instituto Tecnológico de Boca del Río, MX.
authors.forename: I A
authors.initials: IA
authors.lastname: Pérez-Legaspi
authors.affiliation: Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, MX.
authors.forename: R
authors.initials: R
authors.lastname: Rico-Martínez
authors.affiliation: Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, MX.
authors.forename: J L
authors.initials: JL
authors.lastname: Quintanar
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D007306:Insecticides / Q000494:pharmacology*; D008743:Methyl Parathion / Q000494:pharmacology*; D050765:Qa-SNARE Proteins / Q000378:metabolism; D012403:Rotifera / Q000187:drug effects* / Q000201:enzymology; D050827:Syntaxin 1 / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D050765:Qa-SNARE Proteins; D050827:Syntaxin 1; D008743:Methyl Parathion; D012721:Carbaryl