title: An In Vitro Investigation of Platelet-Rich Plasma-Gel as a Cell and Growth Factor Delivery Vehicle for Tissue Engineering.
abstract: Platelet-rich plasma (PRP) has been used for different applications in human and veterinary medicine. Many studies have shown promising therapeutic effects of PRP; however, there are still many controversies regarding its composition, properties, and clinical efficacy. The aim of this study was to evaluate the influence of different platelet concentrations on the rheological properties and growth factor (GF) release profile of PRP-gels. In addition, the viability of incorporated bone marrow-derived human mesenchymal stem cells (MSCs) was investigated. PRP (containing 1000 × 10(3), 2000 × 10(3), and 10,000 × 10(3) platelets/μL) was prepared from human platelet concentrates. Platelet activation and gelification were achieved by addition of human thrombin. Viscoelastic properties of PRP-gels were evaluated by rheological studies. The release of GFs and inflammatory proteins was measured using a membrane-based protein array and enzyme-linked immunosorbent assay. MSC viability and proliferation in PRP-gels were assessed over 7 days by cell viability staining. Cell proliferation was examined using DNA quantification. Regardless of the platelet content, all tested PRP-gels showed effective cross-linking. A positive correlation between protein release and the platelet concentration was observed at all time points. Among the detected proteins, the chemokine CCL5 was the most abundant. The greatest release appeared within the first 4 h after gelification. MSCs could be successfully cultured in PRP-gels over 7 days, with the highest cell viability and DNA content found in PRP-gels with 1000 × 10(3) platelets/μL. The results of this study suggest that PRP-gels represent a suitable carrier for both cell and GF delivery for tissue engineering. Notably, a platelet concentration of 1000 × 10(3) platelets/μL appeared to provide the most favorable environment for MSCs. Thus, the platelet concentration is an important consideration for the clinical application of PRP-gels.
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Jagoda M
authors.initials: JM
authors.lastname: Jalowiec
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Matteo
authors.initials: M
authors.lastname: D'Este
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Jennifer Jane
authors.initials: JJ
authors.lastname: Bara
authors.affiliation: 2 Biologie Fonctionnelle et Adaptative, CNRS UMR 8251, Université Paris Diderot Paris 7 , Paris, France .
authors.forename: Jessica
authors.initials: J
authors.lastname: Denom
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Ursula
authors.initials: U
authors.lastname: Menzel
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Mauro
authors.initials: M
authors.lastname: Alini
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Sophie
authors.initials: S
authors.lastname: Verrier
authors.affiliation: 1 AO Research Institute Davos , Davos, Switzerland .
authors.forename: Marietta
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis; D002470:Cell Survival; D002478:Cells, Cultured; D004058:Diffusion; D004343:Drug Implants / Q000008:administration & dosage / Q000138:chemical synthesis*; D055119:Elastic Modulus; D005782:Gels / Q000737:chemistry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000737:chemistry; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds; D014783:Viscosity
chemical_list: D001672:Biocompatible Materials; D004343:Drug Implants; D005782:Gels; D036341:Intercellular Signaling Peptides and Proteins