title: Nano interfaced biosensor for detection of choline in triple negative breast cancer cells.
abstract: Choline, a type of Vitamin B, is an important nutrient in the human body and is involved in key metabolic pathways. Abnormal levels of choline leads to diseased conditions. The levels of choline and its associated compounds are found to be elevated in triple negative breast cancer (TNBC) patients. The choline level ranges from 0.4 to 4.9mmol/kg in TNBC. Thus the detection of choline levels in cells can aid in diagnosing breast cancer. The present work aims to develop a nano-interfaced electrochemical biosensor for the rapid detection of choline in cancer cells. For electrochemical detection, glassy carbon electrode coated with a zinc oxide nano-interface was used as the working electrode. Zinc oxide synthesized by hydrothermal method was characterized using SEM and XRD. The choline oxidase (ChOx) enzyme was immobilized on the nano-interface by drop-casting. Choline oxidase (ChOx) converts choline to betaine and H2O2 in the presence of oxygen. The H2O2 produced was determined amperometrically. The amount of H2O2 produced is directly proportional to concentration of choline present. The sensitivity, selectivity, stability and concentration studies were carried out and quantification of choline in TNBC was also carried out. The results demonstrate that this biosensor has the potential to be developed as a clinical tool for breast cancer detection.
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur 613 401, India; School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Vignesh
authors.initials: V
authors.lastname: Thiagarajan
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur 613 401, India; School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Sasya
authors.initials: S
authors.lastname: Madhurantakam
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur 613 401, India; School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur 613 401, India; School of Electrical & Electronics Engineering, SASTRA University, Thanjavur 613 401, India.
authors.forename: John Bosco
authors.initials: JB
authors.lastname: Balaguru Rayappan
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), SASTRA University, Thanjavur 613 401, India; School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India. Electronic address: umakrishnan@sastra.edu.
authors.forename: Uma
authors.initials: U
authors.lastname: Maheswari Krishnan
mesh_terms: D015374:Biosensing Techniques; D002794:Choline / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D005260:Female; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties; D064726:Triple Negative Breast Neoplasms / Q000737:chemistry* / Q000175:diagnosis*; D014407:Tumor Cells, Cultured; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D002794:Choline; D015034:Zinc Oxide
keywords: Biosensor; Choline; Nano interface; Triple negative breast cancer