title: Induction of pluripotency in human umbilical cord mesenchymal stem cells in feeder layer-free condition.
abstract: Induced Pluripotent Stem Cells (iPSCs) has been produced by the reprogramming of several types of somatic cells through the expression of different sets of transcription factors. This study consists of a technique to obtain iPSCs from human umbilical cord mesenchymal stem cells (UC-MSCs) in a feeder layer-free process using a mini-circle vector containing defined reprogramming genes, Lin28, Nanog, Oct4 and Sox2. The human MSCs transfected with the minicircle vector were cultured in iPSCs medium. Human embryonic stem cell (ESC)-like colonies with tightly packed domelike structures appeared 7-10 days after the second transfection. In the earliest stages, the colonies were green fluorescence protein (GFP)-positive, while upon continuous culture and passage, genuine hiPSC clones expressing GFP were observed. The induced cells, based on the ectopic expression of the pluripotent markers, exhibited characteristics similar to the embryonic stem cells. These iPSCs demonstrated in vitro capabilities for differentiation into the three main embryonic germ layers by embryoid bodies formation. There was no evidence of transgenes integration into the genome of the iPSCs in this study. In conclusion, this method offers a means of producing iPSCs without viral delivery that could possibly overcome ethical concerns and immune rejection in the use of stem cells in medical applications.
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Faculty of Science, University of Manitoba, Winnipeg, Canada.
authors.forename: Nasibeh
authors.initials: N
authors.lastname: Daneshvar
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: rasedee@vet.upm.edu.com.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Rasedee
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Fatemeh Tash
authors.initials: FT
authors.lastname: Shamsabadi
authors.affiliation: German Cancer Research Center, Department of Virus-Associated Tumours (F100), Im Neuenheimer Feld 242, DH-69120 Heidelberg, Germany.
authors.forename: Hassan
authors.initials: H
authors.lastname: Moeini
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Mehrboud
authors.affiliation: Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Faculty of Veterinary Medicine, University of Sulaimany, Sulaimany City, Iraq.
authors.forename: Heshu Sulaiman
authors.initials: HS
authors.lastname: Rahman
authors.affiliation: Faculty of Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mohadeseh Hashem
authors.initials: MH
authors.lastname: Boroojerdi
authors.affiliation: Department of Biomedical Science, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shalini
authors.initials: S
authors.lastname: Vellasamy
mesh_terms: D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology*; D061252:Feeder Cells / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
keywords: Embryoid bodies; Induced Pluripotent Stem Cells (iPSCs); Umbilical cord mesenchymal stem cells