title: Anatomical and Topochemical Aspects of Japanese beech (Fagus crenata) Cell Walls After Treatment with the Ionic Liquid, 1-Ethylpyridinium Bromide.
abstract: Changes in the ultrastructure and chemical components, and their distribution in Japanese beech (Fagus crenata), during the ionic liquid 1-ethylpyridinium bromide ([EtPy][Br]) treatment were examined at the cellular level by light microscopy, scanning electron microscopy, and confocal Raman microscopy. Each of the tissues, including wood fibers, vessels and parenchyma cells treated with [EtPy][Br] showed specific morphological characteristics. Furthermore, lignin can be preferentially liquefied and eluted in [EtPy][Br] from the cell walls when compared to polysaccharides. However, the delignification was heterogeneous on the cell walls as lignin maintained a relatively high-concentration at the compound middle lamella, cell corners, inner surface of the secondary wall, and pits after [EtPy][Br] treatment.
authors.affiliation: Division of Environmental Sciences,Graduate School of Life and Environmental Sciences,Kyoto Prefectural University,Hangi-cho,Shimogamo,Sakyo-ku,Kyoto 606-8522,Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kanbayashi
authors.affiliation: Division of Environmental Sciences,Graduate School of Life and Environmental Sciences,Kyoto Prefectural University,Hangi-cho,Shimogamo,Sakyo-ku,Kyoto 606-8522,Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyafuji
keywords: 1-ethylpyridinium bromide; Japanese beech; cell wall; ionic liquid; wood