title: Opposing intrinsic temporal gradients guide neural stem cell production of varied neuronal fates.
abstract: Neural stem cells show age-dependent developmental potentials, as evidenced by their production of distinct neuron types at different developmental times. Drosophila neuroblasts produce long, stereotyped lineages of neurons. We searched for factors that could regulate neural temporal fate by RNA-sequencing lineage-specific neuroblasts at various developmental times. We found that two RNA-binding proteins, IGF-II mRNA-binding protein (Imp) and Syncrip (Syp), display opposing high-to-low and low-to-high temporal gradients with lineage-specific temporal dynamics. Imp and Syp promote early and late fates, respectively, in both a slowly progressing and a rapidly changing lineage. Imp and Syp control neuronal fates in the mushroom body lineages by regulating the temporal transcription factor Chinmo translation. Together, the opposing Imp/Syp gradients encode stem cell age, specifying multiple cell fates within a lineage.
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Ching-Po
authors.initials: CP
authors.lastname: Yang
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Sugino
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA. Departments of Bioengineering, Electrical Engineering, and Computer Science, and Biophysics Graduate Program, University of California Berkeley, 408C Stanley Hall, Berkeley, CA, USA.
authors.forename: Chi-Cheng
authors.initials: CC
authors.lastname: Fu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Xiaohao
authors.initials: X
authors.lastname: Yao
authors.affiliation: Departments of Bioengineering, Electrical Engineering, and Computer Science, and Biophysics Graduate Program, University of California Berkeley, 408C Stanley Hall, Berkeley, CA, USA.
authors.forename: Luke P
authors.initials: LP
authors.lastname: Lee
authors.affiliation: Howard Hughes Medical Institute, Janelia Research Campus, 19700 Helix Drive, Ashburn, VA, USA. leet@janelia.hhmi.org.
authors.forename: Tzumin
authors.initials: T
authors.lastname: Lee
mesh_terms: D000818:Animals; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development*; D024521:Mushroom Bodies / Q000166:cytology / Q000254:growth & development; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D017423:Sequence Analysis, RNA
chemical_list: C515167:Chinmo protein, Drosophila; D029721:Drosophila Proteins; C498778:Imp protein, Drosophila; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C000602460:Syp protein, Drosophila