title: The conservation and signatures of lincRNAs in Marek's disease of chicken.
abstract: Long intergenic non-coding RNAs (lincRNAs) associated with a number of cancers and other diseases have been identified in mammals, but they are still formidable to be comprehensively identified and characterized. Marek's disease (MD) is a T cell lymphoma of chickens induced by Marek's disease virus (MDV). Here, we used a MD chicken model to develop a precise pipeline for identifying lincRNAs and to determine the roles of lincRNAs in T cell tumorigenesis. More than 1,000 lincRNA loci were identified in chicken bursa. Computational analyses demonstrated that lincRNAs are conserved among different species such as human, mouse and chicken. The putative lincRNAs were found to be associated with a wide range of biological functions including immune responses. Interestingly, we observed distinct lincRNA expression signatures in bursa between MD resistant and susceptible lines of chickens. One of the candidate lincRNAs, termed linc-satb1, was found to play a crucial role in MD immune response by regulating a nearby protein-coding gene SATB1. Thus, our results manifested that lincRNAs may exert considerable influence on MDV-induced T cell tumorigenesis and provide a rich resource for hypothesis-driven functional studies to reveal genetic mechanisms underlying susceptibility to tumorigenesis.
authors.affiliation: Department of Animal &Avian Sciences, University of Maryland, College Park, MD 20742, USA.
authors.forename: Yanghua
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Animal &Avian Sciences, University of Maryland, College Park, MD 20742, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Animal &Avian Sciences, University of Maryland, College Park, MD 20742, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhan
authors.affiliation: USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.
authors.forename: Huanmin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Han
authors.affiliation: Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Gangqing
authors.initials: G
authors.lastname: Hu
authors.affiliation: Systems Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD, 21201, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Animal &Avian Sciences, University of Maryland, College Park, MD 20742, USA.
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Song
mesh_terms: D000818:Animals; D002645:Chickens; D060467:Disease Resistance; D004198:Disease Susceptibility; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology; D025922:Mardivirus / Q000502:physiology; D008380:Marek Disease / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics; D051379:Mice; D005810:Multigene Family; D011201:Poultry Diseases / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D062085:RNA, Long Noncoding / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D018398:Homeodomain Proteins; D036961:Matrix Attachment Region Binding Proteins; D062085:RNA, Long Noncoding; C088144:HoxA protein