title: Novel candidate genes for alcoholism--transcriptomic analysis of prefrontal medial cortex, hippocampus and nucleus accumbens of Warsaw alcohol-preferring and non-preferring rats.
abstract: OBJECTIVE
  Animal models provide opportunity to study neurobiological aspects of human alcoholism. Changes in gene expression have been implicated in mediating brain functions, including reward system and addiction. The current study aimed to identify genes that may underlie differential ethanol preference in Warsaw High Preferring (WHP) and Warsaw Low Preferring (WLP) rats.
  
  
  METHODS
  Microarray analysis comparing gene expression in nucleus accumbens (NAc), hippocampus (HP) and medial prefrontal cortex (mPFC) was performed in male WHP and WLP rats bred for differences in ethanol preference.
  
  
  RESULTS
  Differential and stable between biological repeats expression of 345, 254 and 129 transcripts in NAc, HP and mPFC was detected. Identified genes and processes included known mediators of ethanol response (Mx2, Fam111a, Itpr1, Gabra4, Agtr1a, LTP/LTD, renin-angiotensin signaling pathway), toxicity (Sult1c2a, Ces1, inflammatory response), as well as genes involved in regulation of important addiction-related brain systems such as dopamine, tachykinin or acetylcholine (Gng7, Tac4, Slc5a7).
  
  
  CONCLUSIONS
  The identified candidate genes may underlie differential ethanol preference in an animal model of alcoholism.
  
  
  CONCLUSIONS
  Names of genes are written in italics, while names of proteins are written in standard font. Names of human genes/proteins are written in all capital letters. Names of rodent genes/proteins are written in capital letter followed by small letters.
authors.affiliation: Department of Animal Behaviour, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzebiec, Poland.
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Stankiewicz
authors.affiliation: Software Department, Faculty of Computer Science, Bialystok University of Technology, 15-351 Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Goscik
authors.affiliation: Department of Pharmacology and Physiology of the Nervous System, Institute of Psychiatry and Neurology, 02-957 Warsaw, Poland.
authors.forename: Wanda
authors.initials: W
authors.lastname: Dyr
authors.affiliation: Department of Animal Behaviour, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, 05-552 Jastrzebiec, Poland.
authors.forename: Grzegorz R
authors.initials: GR
authors.lastname: Juszczak
authors.affiliation: First Department of Neurology, Institute of Psychiatry and Neurology, 02-957 Warsaw, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Ryglewicz
authors.affiliation: Department of Animal and Human Physiology, Faculty of Biology, University of Gdansk, 80-308 Gdansk, Poland; Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, LA71130, USA. Electronic address: artur.swiergiel@biol.ug.edu.pl.
authors.forename: Artur H
authors.initials: AH
authors.lastname: Swiergiel
authors.affiliation: Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Wieczorek
authors.affiliation: Department of Pharmacology and Physiology of the Nervous System, Institute of Psychiatry and Neurology, 02-957 Warsaw, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Stefanski
mesh_terms: D000437:Alcoholism / Q000235:genetics*; D000818:Animals; D002755:Choice Behavior; D004195:Disease Models, Animal; D015870:Gene Expression / Q000235:genetics; D020869:Gene Expression Profiling; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009714:Nucleus Accumbens / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
keywords: Alcohol non-preferring WLP rats; Alcohol-preferring WHP rats; Ethanol; Gene expression profiling; Limbic system; Microarray