title: Fast vaporization solid phase microextraction and ion mobility spectrometry: A new approach for determination of creatinine in biological fluids.
abstract: In this work a rapid and simple method for creatinine determination in urine and plasma samples based on aqueous derivatization of creatinine and complete vaporization of sample (as low as 10 µL), followed by ion mobility spectrometry analysis has been proposed. The effect of four important parameters (extraction temperature, total volume of solution, desorption temperature and extraction time) on ion mobility signal has been studied. Under the optimized conditions, the quantitative response of ion mobility spectrometry for creatinine was linear in the range of 0-500 mg L(-1) with a detection limit of 0.6 mg L(-1) in urine and 0-250 mg L(-1) with a detection limit of 2.6 mg L(-1) in plasma sample. The limit of quantitation of creatinine was 2.1 mg L(-1) and 8.7 mg L(-1) in urine and plasma samples, respectively. The relative standard deviation of the method was found to be 13%. The method was successfully applied to the analysis of creatinine in biological samples, showing recoveries from 92% to 104% in urine and 101-110% in plasma samples.
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University G.C., Tehran, Islamic Republic of Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Jafari
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University G.C., Tehran, Islamic Republic of Iran. Electronic address: h-ebrahim@sbu.ac.ir.
authors.forename: Homeira
authors.initials: H
authors.lastname: Ebrahimzadeh
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University G.C., Tehran, Islamic Republic of Iran.
authors.forename: Mohamma Hossein
authors.initials: MH
authors.lastname: Banitaba
mesh_terms: D003404:Creatinine / Q000097:blood* / Q000652:urine*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D010087:Oxides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D052617:Solid Phase Microextraction; D013057:Spectrum Analysis / Q000379:methods; D014835:Volatilization
chemical_list: D010087:Oxides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006108:Graphite; D003404:Creatinine
keywords: Cold fiber solid phase microextraction; Creatinine; Extraction theory; Fast vaporization; Ion mobility spectrometry; Method development