title: Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique.
abstract: Traditional maceration method was used for the extraction of polyphenols from chokeberry (Aronia melanocarpa) dried fruit, and the effects of several extraction parameters on the total phenolics and anthocyanins contents were studied. Various solvents, particle size, solid-solvent ratio and extraction time have been investigated as independent variables in two level factorial design. Among examined variables, time was not statistically important factor for the extraction of polyphenols. The optimal extraction conditions were maceration of 0.75mm size berries by 50% ethanol, with solid-solvent ratio of 1:20, and predicted values were 27.7mgGAE/g for total phenolics and 0.27% for total anthocyanins. Under selected conditions, the experimental total phenolics were 27.8mgGAE/g, and total anthocyanins were 0.27%, which is in agreement with the predicted values. In addition, a complementary quantitative analysis of individual phenolic compounds was performed using HPLC method. The study indicated that maceration was effective and simple technique for the extraction of bioactive compounds from chokeberry fruit.
authors.affiliation: Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
authors.forename: Nada
authors.initials: N
authors.lastname: Ćujić
authors.affiliation: Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Šavikin
authors.affiliation: Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia. Electronic address: tjankovic@mocbilja.rs.
authors.forename: Teodora
authors.initials: T
authors.lastname: Janković
authors.affiliation: Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Pljevljakušić
authors.affiliation: Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
authors.forename: Gordana
authors.initials: G
authors.lastname: Zdunić
authors.affiliation: Faculty of Pharmacy, Department of Pharmaceutical Technology, University of Belgrade, Vojvode Stepe 459, 11000 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Ibrić
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D046936:Photinia / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis*
chemical_list: D000872:Anthocyanins; D010636:Phenols; D059808:Polyphenols
keywords: Aronia melanocarpa; Extraction; Factorial design; HPLC; Maceration; Polyphenols