title: Differential mesocorticolimbic responses to palatable food in binge eating prone and binge eating resistant female rats.
abstract: Binge eating is a key symptom of many eating disorders (e.g. binge eating disorder, bulimia nervosa, anorexia nervosa binge/purge type), yet the neurobiological underpinnings of binge eating are poorly understood. The mesocorticolimbic reward circuit, including the nucleus accumbens and the medial prefrontal cortex, is likely involved because this circuit mediates the hedonic value and incentive salience of palatable foods (PF). Here we tested the hypothesis that higher propensity for binge eating is associated with a heightened response (i.e., Fos induction) of the nucleus accumbens and medial prefrontal cortex to PF, using an animal model that identifies binge eating prone (BEP) and binge eating resistant (BER) rats. Forty adult female Sprague-Dawley rats were given intermittent access to PF (high fat pellets) 3×/week for 3 weeks. Based on a pattern of either consistently high or consistently low PF consumption across these feeding tests, 8 rats met criteria for categorization as BEP, and 11 rats met criteria for categorization as BER. One week after the final feeding test, BEP and BER rats were either exposed to PF in their home cages or were given no PF in their home cages for 1h prior to perfusion, leading to three experimental groups for the Fos analysis: BEPs given PF, BERs given PF, and a No PF control group. The total number of Fos-immunoreactive (Fos-ir) cells in the nucleus accumbens core and shell, and the cingulate, prelimbic, and infralimbic regions of the medial prefrontal cortex was estimated by stereological analysis. PF induced higher Fos expression in the nucleus accumbens shell and core and in the prelimbic and infralimbic cortex of BEP rats compared to No PF controls. Throughout the nucleus accumbens and medial prefrontal cortex, PF induced higher Fos expression in BEP than in BER rats, even after adjusting for differences in PF intake. Differences in the neural activation pattern between BEP and BER rats were more robust in prefrontal cortex than in nucleus accumbens. These data confirm that PF activates brain regions responsible for encoding the incentive salience and hedonic properties of PF, and suggest that binge eating proneness is associated with enhanced responses to PF in brain regions that exert executive control over food reward.
authors.affiliation: Neuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, United States. Electronic address: sincla61@msu.edu.
authors.forename: Elaine B
authors.initials: EB
authors.lastname: Sinclair
authors.affiliation: Department of Psychology, University of Nevada, Las Vegas, 4505 S. Maryland Parkway, Las Vegas, NV 89154-5030, United States.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Culbert
authors.affiliation: Neuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, United States.
authors.forename: Dana R
authors.initials: DR
authors.lastname: Gradl
authors.affiliation: Department of Pharmacology, Howard University College of Medicine, 520 W Street, NW Suite 3408, Washington, DC 20059, United States.
authors.forename: Kimberlei A
authors.initials: KA
authors.lastname: Richardson
authors.affiliation: Department of Psychology, Michigan State University, 316 Physics Road, East Lansing, MI 48824, United States.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Klump
authors.affiliation: Neuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, United States.
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Sisk
mesh_terms: D000821:Animal Feed; D000818:Animals; D056912:Binge-Eating Disorder / Q000503:physiopathology*; D004195:Disease Models, Animal; D004435:Eating / Q000502:physiology; D005247:Feeding Behavior / Q000502:physiology; D005260:Female; D005502:Food; D020022:Genetic Predisposition to Disease; D007150:Immunohistochemistry; D009714:Nucleus Accumbens / Q000503:physiopathology*; D017397:Prefrontal Cortex / Q000503:physiopathology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012201:Reward; D013045:Species Specificity
chemical_list: D016760:Proto-Oncogene Proteins c-fos
keywords: Binge eating; Eating disorders; Medial prefrontal cortex; Nucleus accumbens; Palatable food; Reward