title: Use of a miniature laboratory fresh cheese model for investigating antimicrobial activities.
abstract: Hispanic-style fresh cheeses, such as queso fresco, have relatively low salt content, high water activity, and near neutral pH, which predisposes them to growth of Listeria monocytogenes. Biosafety constraints limit the incorporation of L. monocytogenes into cheeses manufactured via traditional methods in challenge studies, so few have focused on in situ testing of novel antimicrobials in fresh cheeses. We have developed a modular, miniaturized laboratory-scale queso fresco model for testing the incorporation of novel antilisterials. We have demonstrated the assessment of the antilisterials nisin and ferulic acid, alone and in combination, at various levels. Our results support the inhibitory effects of ferulic acid in cheese, against both L. monocytogenes and its common surrogate Listeria innocua, and we provide preliminary evaluation of its consumer acceptability.
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801.
authors.forename: M L
authors.initials: ML
authors.lastname: Van Tassell
authors.affiliation: Programa de Posgrado en Alimentos del Centro de la República, Universidad Autónoma de Querétaro, Querétaro, QRO 76010, México.
authors.forename: L A
authors.initials: LA
authors.lastname: Ibarra-Sánchez
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801.
authors.forename: S R
authors.initials: SR
authors.lastname: Takhar
authors.affiliation: Programa de Posgrado en Alimentos del Centro de la República, Universidad Autónoma de Querétaro, Querétaro, QRO 76010, México.
authors.forename: S L
authors.initials: SL
authors.lastname: Amaya-Llano
authors.affiliation: Department of Food Science and Human Nutrition, University of Illinois, Urbana 61801. Electronic address: mille216@illinois.edu.
authors.forename: M J
authors.initials: MJ
authors.lastname: Miller
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002611:Cheese / Q000032:analysis / Q000382:microbiology*; D003373:Coumaric Acids / Q000494:pharmacology; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development*; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D003373:Coumaric Acids; D009561:Nisin; C004999:ferulic acid
keywords: Listeria monocytogenes; ferulic acid; minimum inhibitory concentration; nisin; queso fresco cheese