title: Protective effect of Rhodiola rosea polysaccharides on cryopreserved boar sperm.
abstract: Cryopreservation brings sublethal damage to sperm, resulting in reduced fertile life of sperm. Rhodiola rosea polysaccharides (RPs) have antiviral, antioxidant and antitumor activities. In the present study, the cryoprotective effect of RPs on boar sperm quality parameters after frozen-thawed process was investigated. Boar sperm was cryopreserved in the extender with RPs added at concentrations of 0 (used as control), 2, 4, 6, 8 and 10mg/L and their effects on the quality of frozen-thawed boar sperm were assessed. Addition of RPs significantly improved sperm motility, mitochondrial activity, acrosomal integrity, plasma membrane integrity, superoxide dismutase and glutathione peroxidase activity and decreased sperm malonaldehyde level (p<0.05). The results indicated that the addition of RPs to the freezing extender decreased the cryodamage to the boar sperm.
authors.affiliation: Center for Reproduction and Genetics, Suzhou Hospital affiliated to Nanjing Medical University, 26 Daoqian Street, Suzhou 215002, China; Department of Urology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Shen-Min
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Center for Reproduction and Genetics, Suzhou Hospital affiliated to Nanjing Medical University, 26 Daoqian Street, Suzhou 215002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Urology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Duan-Gai
authors.initials: DG
authors.lastname: Wen
authors.affiliation: Department of Urology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Hou
authors.affiliation: Center for Reproduction and Genetics, Suzhou Hospital affiliated to Nanjing Medical University, 26 Daoqian Street, Suzhou 215002, China. Electronic address: lihaiboszh@163.com.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Li
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D029771:Rhodiola; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine
chemical_list: D003451:Cryoprotective Agents; D011134:Polysaccharides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Boar sperm; Cryopreservation; Polysaccharides