title: Validity and reliability of autofluorescence-based quantification method of dental plaque.
abstract: BACKGROUND
  The aim of this study was to evaluate validity and reliability of autofluorescence-based plaque quantification (APQ) method.
  
  
  METHODS
  The facial surfaces of 600 sound anterior teeth of 50 subjects were examined. The subjects received dental plaque examination using Turesky modified Quigley Hein plaque index (QHI) and Silness & Löe plaque index (SLI). The autofluorescence images were taken before the plaque examination with Quantitative Light-induced Fluorescence-Digital, and plaque percent index (PPI) was calculated. Correlation between two existing plaque indices and the PPI of the APQ method was evaluated to find which level of plaque redness on tooth (ΔR) by the APQ method shows the highest correlation. The area under the ROC curve (AUC) analysis and intra- and inter-examiner reliability tests were performed.
  
  
  RESULTS
  The PPIΔR20 of the APQ method showed a moderate correlation with two existing plaque indices (rho of QHI=0.48, SLI=0.51). This methodology fell in the fair category and it had an excellent reliability. The APQ method also showed possibility to detect heavy plaque with fair validity.
  
  
  CONCLUSIONS
  The APQ method demonstrated excellent reliability, and fair validity, compared with 2 conventional indices. The plaque quantification described has the potential to be used in clinical evaluation of oral hygiene procedures.
authors.affiliation: Department of Preventive Dentistry & Public Oral Health, Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea; Department of Dental Hygiene, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Han
authors.affiliation: Department of Preventive Dentistry & Public Oral Health, Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Department of Preventive Dentistry & Public Oral Health, Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Hae-Youn
authors.initials: HY
authors.lastname: Ko
authors.affiliation: Department of Preventive Dentistry & Public Oral Health, Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Kwon
authors.affiliation: Department of Preventive Dentistry & Public Oral Health, Oral Science Research Institute, Brain Korea 21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea. Electronic address: drkbi@yuhs.ac.
authors.forename: Baek-Il
authors.initials: BI
authors.lastname: Kim
mesh_terms: D000328:Adult; D003753:Dental Health Surveys / Q000295:instrumentation*; D003773:Dental Plaque / Q000503:physiopathology*; D003774:Dental Plaque Index; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D061848:Optical Imaging / Q000295:instrumentation*; D012372:ROC Curve; D015203:Reproducibility of Results
keywords: Dental plaque; Fluorescence; QLF; Quantification