title: Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications.
abstract: Monodisperse poly(glycidyl methacrylate) (PGMA) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. The effects of various reaction parameters on the particle properties were investigated. The particle size was controlled in the range of 350-420 nm. To introduce carboxyl groups, the PGMA nanospheres were hydrolyzed and oxidized with KMnO4. Subsequently, the enzyme horseradish peroxidase (HRP) and the electron mediator thionine were covalently attached to the PGMA nanospheres to obtain an antibody indicator suitable for enzyme-based electrochemical immunosensors. Combined HRP and thionine binding to the nanospheres had beneficial effects for the labeling efficiency and at the same time prevented the formation of soluble electron mediators.
authors.affiliation: Institute of Macromolecular Chemistry AS CR , Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Beata
authors.initials: B
authors.lastname: Zasonská
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice , Studentská 573, 532 10 Pardubice, Czech Republic.|Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice , Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Čadková
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice , Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Aneta
authors.initials: A
authors.lastname: Kovářová
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice , Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bílková
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice , Studentská 573, 532 10 Pardubice, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Korecká
authors.affiliation: Institute of Macromolecular Chemistry AS CR , Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004583:Electrons; D004800:Enzymes, Immobilized / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006868:Hydrolysis; D008855:Microscopy, Electron, Scanning; D054118:Nanospheres / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D010100:Oxygen / Q000737:chemistry; D010316:Particle Size; D010640:Phenothiazines / Q000737:chemistry*; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000906:Antibodies; D004800:Enzymes, Immobilized; D010640:Phenothiazines; D011109:Polymethacrylic Acids; D011137:Polystyrenes; C042535:polyglycidyl methacrylate; D006735:Horseradish Peroxidase; D010100:Oxygen; C009469:thionine
keywords: biosensor; electrochemistry; glycidyl methacrylate; horseradish peroxidase; nanospheres; thionine