title: Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens.
abstract: The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D(3) Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.
authors.affiliation: Laboratory Alliance of Central New York, Liverpool, New York, USA SUNY Upstate Medical University, Syracuse, New York, USA PaulGranatoPhD@lacny.com.
authors.forename: Paul A
authors.identifier: http://orcid.org/0000-0002-5068-1137
authors.initials: PA
authors.lastname: Granato
authors.affiliation: Laboratory Alliance of Central New York, Liverpool, New York, USA.
authors.forename: Brenda R
authors.initials: BR
authors.lastname: Alkins
authors.affiliation: Cleveland Clinic, Cleveland, Ohio, USA.
authors.forename: Belinda
authors.initials: B
authors.lastname: Yen-Lieberman
authors.affiliation: Penn State Hershey Medical Center, Hershey, Pennsylvania, USA.
authors.forename: Wallace H
authors.initials: WH
authors.lastname: Greene
authors.affiliation: The Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Connolly
authors.affiliation: The Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Blake W
authors.identifier: http://orcid.org/0000-0002-4111-048X
authors.initials: BW
authors.lastname: Buchan
authors.affiliation: The Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Ledeboer
mesh_terms: D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009092:Mucous Membrane / Q000821:virology*; D012680:Sensitivity and Specificity; D012867:Skin / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods*