title: Miniaturization of High-Throughput Epigenetic Methyltransferase Assays with Acoustic Liquid Handling.
abstract: Epigenetics continues to emerge as an important target class for drug discovery and cancer research. As programs scale to evaluate many new targets related to epigenetic expression, new tools and techniques are required to enable efficient and reproducible high-throughput epigenetic screening. Assay miniaturization increases screening throughput and reduces operating costs. Echo liquid handlers can transfer compounds, samples, reagents, and beads in submicroliter volumes to high-density assay formats using only acoustic energy-no contact or tips required. This eliminates tip costs and reduces the risk of reagent carryover. In this study, we demonstrate the miniaturization of a methyltransferase assay using Echo liquid handlers and two different assay technologies: AlphaLISA from PerkinElmer and EPIgeneous HTRF from Cisbio.
authors.affiliation: Labcyte Inc., Sunnyvale, CA, USA bedwards@labcyte.com.
authors.forename: Bonnie
authors.initials: B
authors.lastname: Edwards
authors.affiliation: Labcyte Inc., Sunnyvale, CA, USA.
authors.forename: John
authors.initials: J
authors.lastname: Lesnick
authors.affiliation: Labcyte Inc., Sunnyvale, CA, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Cisbio Bioassays, Bedford, MD, USA.
authors.forename: Nga
authors.initials: N
authors.lastname: Tang
authors.affiliation: BMG LABTECH, Cary, NC, USA.
authors.forename: Carl
authors.initials: C
authors.lastname: Peters
mesh_terms: D000162:Acoustics; D020811:Biomedical Technology / Q000379:methods*; D057890:Epigenomics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D008780:Methyltransferases / Q000032:analysis*; D008904:Miniaturization / Q000379:methods*; D012996:Solutions
chemical_list: D012996:Solutions; D008780:Methyltransferases
keywords: ADE (acoustic droplet ejection); AlphaLISA; HTRF; epigenetics; liquid handling; methyltransferase