title: Transcriptional Profiling of Resistant and Susceptible Buffalograsses in Response to Blissus occiduus (Hemiptera: Blissidae) Feeding.
abstract: Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with susceptible control plants. Defense-related transcripts, including two peroxidase genes, two catalase genes, several cytochrome P450 transcripts, a glutathione s-transferase, and a WRKY gene were upregulated within the Prestige transcriptome in response to chinch bug feeding. The majority of observed transcripts with oxidoreductase activity, including nine peroxidase genes and a catalase gene, were downregulated in 378 in response to initial chinch bug feeding. The observed difference in transcript expression between these two buffalograss genotypes provides insight into the mechanism(s) of resistance, specifically buffalograss tolerance to chinch bug feeding.
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE 68583.
authors.forename: Crystal
authors.initials: C
authors.lastname: Ramm
authors.affiliation: Department of Biology, University of Nebraska, Omaha, NE 68182.
authors.forename: Michael
authors.initials: M
authors.lastname: Wachholtz
authors.affiliation: Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583.
authors.forename: Keenan
authors.initials: K
authors.lastname: Amundsen
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE 68583.
authors.forename: Teresa
authors.initials: T
authors.lastname: Donze
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE 68583. thengmoss2@unl.edu.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Heng-Moss
authors.affiliation: Department of Biology, University of Nebraska, Kearney, NE 68449.
authors.forename: Paul
authors.initials: P
authors.lastname: Twigg
authors.affiliation: Grain, Forage and Bioenergy Research Unit, USDA-ARS & Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Palmer
authors.affiliation: Grain, Forage and Bioenergy Research Unit, USDA-ARS & Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583.
authors.forename: Gautam
authors.initials: G
authors.lastname: Sarath
authors.affiliation: Department of Entomology, University of Nebraska, Lincoln, NE 68583.
authors.forename: Fred
authors.initials: F
authors.lastname: Baxendale
mesh_terms: D000818:Animals; D020387:Food Chain; D006430:Hemiptera / Q000502:physiology*; D060434:Herbivory; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D010940:Plant Proteins
keywords: buffalograss; catalase; chinch bug; peroxidase; transcriptome