title: Microtubule-driven nuclear rotations promote meiotic chromosome dynamics.
abstract: At the onset of meiosis, each chromosome needs to find its homologue and pair to ensure proper segregation. In Drosophila, pairing occurs during the mitotic cycles preceding meiosis. Here we show that germ cell nuclei undergo marked movements during this developmental window. We demonstrate that microtubules and Dynein are driving nuclear rotations and are required for centromere pairing and clustering. We further found that Klaroid (SUN) and Klarsicht (KASH) co-localize with centromeres at the nuclear envelope and are required for proper chromosome motions and pairing. We identified Mud (NuMA in vertebrates) as co-localizing with centromeres, Klarsicht and Klaroid. Mud is also required to maintain the integrity of the nuclear envelope and for the correct assembly of the synaptonemal complex. Our findings reveal a mechanism for chromosome pairing in Drosophila, and indicate that microtubules, centrosomes and associated proteins play a crucial role in the dynamic organization of chromosomes inside the nucleus.
authors.affiliation: Department of Genetics and Developmental Biology, Institut Curie, F-75248 Paris, France.|CNRS UMR3215, Inserm, U934 F-75248 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Christophorou
authors.affiliation: Department of Genetics and Developmental Biology, Institut Curie, F-75248 Paris, France.|CNRS UMR3215, Inserm, U934 F-75248 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rubin
authors.affiliation: Laboratoire Physico-Chimie, Institut Curie, F-75248 Paris, France.|CNRS UMR 168, UPMC, F-75248 Paris, France.
authors.forename: Isabelle
authors.identifier: http://orcid.org/0000-0003-2343-3784
authors.initials: I
authors.lastname: Bonnet
authors.affiliation: Department of Genetics and Developmental Biology, Institut Curie, F-75248 Paris, France.|CNRS UMR3215, Inserm, U934 F-75248 Paris, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Piolot
authors.affiliation: Department of Genetics and Developmental Biology, Institut Curie, F-75248 Paris, France.|CNRS UMR3215, Inserm, U934 F-75248 Paris, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Arnaud
authors.affiliation: Department of Genetics and Developmental Biology, Institut Curie, F-75248 Paris, France.|CNRS UMR3215, Inserm, U934 F-75248 Paris, France.
authors.forename: Jean-René
authors.initials: JR
authors.lastname: Huynh
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D018385:Centrosome / Q000378:metabolism; D023902:Chromosome Pairing; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008540:Meiosis; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D034622:RNA Interference; D012399:Rotation; D013573:Synaptonemal Complex; D059008:Time-Lapse Imaging / Q000379:methods
chemical_list: D029721:Drosophila Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C411670:Mud protein, Drosophila; D009419:Nerve Tissue Proteins; C401521:klar protein, Drosophila; C554441:koi protein, Drosophila; D004398:Dyneins