title: Single-cell epigenomics: techniques and emerging applications.
abstract: Epigenomics is the study of the physical modifications, associations and conformations of genomic DNA sequences, with the aim of linking these with epigenetic memory, cellular identity and tissue-specific functions. While current techniques in the field are characterizing the average epigenomic features across large cell ensembles, the increasing interest in the epigenetics within complex and heterogeneous tissues is driving the development of single-cell epigenomics. We review emerging single-cell methods for capturing DNA methylation, chromatin accessibility, histone modifications, chromosome conformation and replication dynamics. Together, these techniques are rapidly becoming a powerful tool in studies of cellular plasticity and diversity, as seen in stem cells and cancer.
authors.affiliation: Human Molecular Genetics and Biochemistry, Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.|Functional Genomics and Childhood Leukemia Research Section, Cancer Research Center, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat-Gan 52621, Israel.|Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot 7610001, Israel.
authors.forename: Omer
authors.initials: O
authors.lastname: Schwartzman
authors.affiliation: Department of Computer Science and Applied Mathematics, and Department of Biological Regulation, Weizmann Institute, Rehovot 7610001, Israel.
authors.forename: Amos
authors.initials: A
authors.lastname: Tanay
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly; D019175:DNA Methylation; D004261:DNA Replication; D057890:Epigenomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006657:Histones; D006801:Humans; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones