title: Computational elucidation of potential antigenic CTL epitopes in Ebola virus.
abstract: Cell-mediated immunity is important for the control of Ebola virus infection. We hypothesized that those HLA A0201 and HLA B40 restricted epitopes derived from Ebola virus proteins, would mount a good antigenic response. Here we employed an immunoinformatics approach to identify specific 9mer amino acid which may be capable of inducing a robust cell-mediated immune response in humans. We identified a set of 28 epitopes that had no homologs in humans. Specifically, the epitopes derived from NP, RdRp, GP and VP40 share population coverage of 93.40%, 84.15%, 74.94% and 77.12%, respectively. Based on the other HLA binding specificity and population coverage, seven novel promiscuous epitopes were identified. These 7 promiscuous epitopes from NP, RdRp and GP were found to have world-wide population coverage of more than 95% indicating their potential significance as useful candidates for vaccine design. Epitope conservancy analysis also suggested that most of the peptides are highly conserved (100%) in other virulent Ebola strain (Mayinga-76, Kikwit-95 and Makona-G3816- 2014) and can therefore be further investigated for their immunological relevance and usefulness as vaccine candidates.
authors.affiliation: Department of Bioinformatics, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
authors.forename: Manas R
authors.initials: MR
authors.lastname: Dikhit
authors.affiliation: Department of Biotechnology and Department of Pharmacoinformatics, National Institutes of Pharmaceutical Education and Research, Hajipur 844102, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology and Department of Pharmacoinformatics, National Institutes of Pharmaceutical Education and Research, Hajipur 844102, India; Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
authors.lastname: Vijaymahantesh
authors.affiliation: Institute for Protein Research, Osaka University, Suita 5650871, Japan.
authors.forename: Bikash R
authors.initials: BR
authors.lastname: Sahoo
authors.affiliation: Department of Bioinformatics, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India; Department of Biotechnology and Department of Pharmacoinformatics, National Institutes of Pharmaceutical Education and Research, Hajipur 844102, India.
authors.forename: Rani
authors.initials: R
authors.lastname: Mansuri
authors.affiliation: Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Amit
authors.affiliation: Department of Bioinformatics, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India; Department of Biotechnology and Department of Pharmacoinformatics, National Institutes of Pharmaceutical Education and Research, Hajipur 844102, India.
authors.forename: Md
authors.initials: M
authors.lastname: Yousuf Ansari
authors.affiliation: Department of Bioinformatics, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
authors.forename: Ganesh C
authors.initials: GC
authors.lastname: Sahoo
authors.affiliation: Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India.
authors.forename: Sanjiva
authors.initials: S
authors.lastname: Bimal
authors.affiliation: Dept. of Molecular Parasitology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India. Electronic address: drpradeep.das@gmail.com.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Das
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology*; D029043:Ebolavirus / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019142:Hemorrhagic Fever, Ebola / Q000235:genetics / Q000276:immunology* / Q000821:virology; D006801:Humans; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D011487:Protein Conformation; D040901:Proteomics / Q000379:methods; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen; D015235:HLA-B Antigens; D010455:Peptides
keywords: CD8+ T cell; Ebola virus; Epitope; Immunoinformatics; MHC class I