title: Electrochemical preparation of a molecularly imprinted polypyrrole modified pencil graphite electrode for the determination of phenothiazine in model and real biological samples.
abstract: A sensitive electrochemical sensor for determination of phenothiazine (PTZ) was introduced based on molecularly imprinted polymer (MIP) film. A computational study was performed to evaluate the template-monomer geometry and interaction energy in the prepolymerization mixture. The electrode was prepared during electropolymerization of pyrrole (Py) on a pencil graphite electrode (PGE) by cyclic voltammetry (CV) technique. The quantitative measurements were performed using differential pulse voltammetry (DPV) in Britton-Robinson (BR) buffer solutions using 60% (v/v) acetonitrile-water (ACN/H2O) binary solvent. The effect of important parameters like pH, monomer concentration, number of cycles, etc on the efficiency of MIP electrode was optimized and the calibration curve was plotted at optimal conditions. Two dynamic linear ranges of 1-300 µmol L(-1) and 0.5-10 mmol L(-1) were observed. The detection limit (based on S/N=3) of PTZ was obtained 3×10(-7) mol L(-1). The MIP/PGE has been successfully applied as a selective sensor for fast and accurate determination of PTZ in some model and real biological samples.
authors.affiliation: Department of Chemistry, Payame Noor University (PNU), Mashhad, Iran; Department of Chemistry, Payame Noor University, P.B. 19395-4697, Tehran 19569, Iran. Electronic address: aziz_nezhadali@pnu.ac.ir.
authors.forename: Azizollah
authors.initials: A
authors.lastname: Nezhadali
authors.affiliation: Department of Chemistry, Payame Noor University, P.B. 19395-4697, Tehran 19569, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Rouki
authors.affiliation: Department of Chemical and Petroleum Engineering , Sharif University of Technology, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nezhadali
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D055664:Electrochemical Techniques; D004566:Electrodes; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D054802:Molecular Imprinting; D010640:Phenothiazines / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017747:Seafood / Q000032:analysis; D012756:Sheep
chemical_list: D000981:Antiprotozoal Agents; D010640:Phenothiazines; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006108:Graphite; C031637:phenothiazine
keywords: Electropolymerization; Molecularly imprinted polymer; Pencil graphite electrode; Phenothiazine (PTZ); Polypyrrole