title: Biodegradation of Azo Dye Disperse Orange S-RL by a Newly Isolated Strain Acinetobacter sp. SRL8.
abstract: The strain SRL8, which could decolorize the azo dye disperse orange S-RL (S-RL), was first isolated from sludge and identified as Acinetobacter sp. through physiobiochemical identification and 16S rRNA gene sequences. The effects of temperature, pH, dye concentration, O2, and glucose concentration on S-RL decolorization by the strain SRL8 were studied. The optimal conditions were 30 °C, pH 7.0, 4g·L(-1) of inoculation (wet cells), and microaerophilic incubation. The decolorization percentage for S-RL by the strain SRL8 could reach 90.2% under optimal conditions. The strain SRL8 was highly tolerant to the azo dye SRL up to 300 mg·L(-1) and it had a broad decolorizing spectrum. According to the Monod equation, kinetic parameters of decolorization by SRL8 were calculated. The vmax and Km were 5.57×10(-3) h(-1) and 14.53 mg·L(-1), respectively.
authors.affiliation: Laboratory of Applied Microbiology, Changzhou University, Changzhou, China, 213164, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Cai
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Ma
authors.forename: Jinyan
authors.initials: J
authors.lastname: Cai
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhu
authors.forename: Guanghua
authors.initials: G
authors.lastname: Yang
authors.forename: Xiyue
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000150:Acinetobacter / Q000378:metabolism* / Q000648:ultrastructure; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D015394:Molecular Structure; D014874:Water Pollutants, Chemical
chemical_list: D001391:Azo Compounds; C000602573:Disperse Orange S-RL; D014874:Water Pollutants, Chemical