title: Expression pattern of invasion-related molecules in the peritumoral brain.
abstract: OBJECTIVE
  The effectiveness of therapy of intracerebral neoplasms is mainly influenced by the invasive behaviour of the tumour. The peritumoral invasion depends on the interaction between the tumour cells and the extracellular matrix (ECM) of the surrounding brain. The invading tumour cells induce change in the activity of proteases, synthases and expression of ECM-components. These alterations in the peritumoral ECM are in connection to the highly different invasiveness of gliomas and metastatic brain tumours. To understand the fairly modified invasive potential of anaplastic intracerebral tumours of different origin, the effect of tumour on the peritumoral ECM and alterations of invasion related ECM components in the peritumoral brain were evaluated.
  
  
  METHODS
  For this reason the mRNA expression of 19 invasion-related molecules by quantitative reverse transcriptase polymerase chain reaction was determined in normal brain tissue (Norm), in the peritumoral brain tissue of glioblastoma (peri-GBM) and of intracerebral adenocarcinoma metastasis (peri-Met). To evaluate the translational expression of the investigated molecules protein levels were determined by targeted proteomic methods.
  
  
  RESULTS
  Establishing the invasion pattern of the investigated tissue samples 8 molecules showed concordant difference at mRNA and protein levels in the peri-GBM and peri-Met, 11 molecules in the peri-Met and normal brain and 12 in the peri-GBM and normal brain comparison.
  
  
  CONCLUSIONS
  Our results bring some ECM molecules into focus that probably play key role in arresting tumour cell invasion around the metastatic tumour, and also in the lack of impeding tumour cell migration in case of glioblastoma.
authors.affiliation: Department of Neurosurgery, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary. Electronic address: neurosurgery.debrecen@freemail.hu.
authors.forename: Álmos
authors.initials: Á
authors.lastname: Klekner
authors.affiliation: Department of Neurosurgery, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Hutóczki
authors.affiliation: Department of Neurosurgery, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Virga
authors.affiliation: Department of Neurosurgery, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Reményi-Puskár
authors.affiliation: Department of Oncology, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Tóth
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Beáta
authors.initials: B
authors.lastname: Scholtz
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Csősz
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Gergő
authors.initials: G
authors.lastname: Kalló
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Steiner
authors.affiliation: Division of Neuropathology, Institute of Pathology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Hortobágyi
authors.affiliation: Department of Neurosurgery, University of Debrecen, Clinical Center, Nagyerdei krt. 98, Debrecen 4032, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Bognár
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D015870:Gene Expression; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D009362:Neoplasm Metastasis / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger
keywords: Brain metastasis; Extracellular matrix; Gene expression; Glioblastoma; Invasion; Peritumoral brain