title: Dynamic modeling of in vitro lipid digestion: individual fatty acid release and bioaccessibility kinetics.
abstract: The aim of this study was to gain knowledge about the role of triacylglycerol (TAG) composition in fatty acids (FA) of o/w emulsions on both the pancreatic lipolysis kinetics and the bioaccessibility of released products (i.e. contained within the bile salt micellar phase). A mathematical model was developed and its predictions were compared to a set of experimental data obtained during an in vitro digestion of a whey protein stabilized emulsion. Modeling results show that FA residues of TAG were hydrolyzed at specific rates, inducing different bioaccessibility kinetics. The estimated lipolysis rate constants of the studied FA (C8:0, C10:0≫C18:1 n-9≫C12:0>C14:0>C16:0≈C16:1 n-7>C22:6 n-3) were in close agreement with the available literature on the substrate specificity of pancreatic lipase. Results also suggest that lipolysis products are very rapidly solubilized in the bile salt mixed micelles with no fractionation according to the FA carbon chain.
authors.affiliation: INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France; AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France.
authors.forename: T M
authors.initials: TM
authors.lastname: Giang
authors.affiliation: INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France; AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France.
authors.forename: S
authors.initials: S
authors.lastname: Gaucel
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France.
authors.forename: P
authors.initials: P
authors.lastname: Brestaz
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Anton
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44300 Nantes, France.
authors.forename: A
authors.initials: A
authors.lastname: Meynier
authors.affiliation: INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France; AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France.
authors.forename: I C
authors.initials: IC
authors.lastname: Trelea
authors.affiliation: INRA, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France; AgroParisTech, UMR782 Génie et Microbiologie des Procédés Alimentaires, F-78850 Thiverval Grignon, France. Electronic address: steven.le-feunteun@grignon.inra.fr.
authors.forename: S
authors.initials: S
authors.lastname: Le Feunteun
mesh_terms: D004063:Digestion; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis; D008954:Models, Biological; D008962:Models, Theoretical; D010179:Pancreas / Q000201:enzymology; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D014280:Triglycerides; D008049:Lipase
keywords: DHA; Digestion; Emulsion; Pancreatic lipase; Simulation; Substrate specificity