title: Separation of capsaicin from capsaicinoids by macroporous resin adsorption chromatography.
abstract: The aim of present study is to develop an efficient and low-cost method for capsaicin production isolated from capsaicinoids by macroporous resin adsorption chromatography. HZ816 resin has shown the best adsorption and desorption capacities for capsaicin among other resins. To optimize the operating parameters for separation, initial concentration, diameter-to-height ratio, mobile phase ratio, and crystallization method were investigated. When capsaicinoids solution (5 g/L) was loaded onto the column (diameter-to-height ratio = 1:12) with ethanol/1% w/w NaOH (4:6, v/v) as the mobile phase, capsaicin was purified most effectively. By using acid neutralization as the crystallization method, the purity of capsaicin improved from 90.3 to 99.5% with 82.3% yield. In conclusion, this study provides a simple and low-cost method for the industrial-scale production of high-purity capsaicin.
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Changxia
authors.initials: C
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Ruican
authors.initials: R
authors.lastname: Liu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Chunqiao
authors.initials: C
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D002211:Capsaicin / Q000737:chemistry / Q000302:isolation & purification*; D002617:Chemical Industry / Q000379:methods*; D002853:Chromatography, Liquid; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D012117:Resins, Synthetic; D002211:Capsaicin
keywords: Adsorption chromatography; Capsaicin; Capsaicinoids; Chili; Macroporous resin