title: Fast and simple MEKC sweeping method for determination of thiosulfate in urine.
abstract: A new method for determination of thiosulfate in human urine has been developed and validated. Analytical procedure is very simple and consists of only few steps: derivatization of thiosulfate with 2-chloro-1-methylquinolinium tetrafluoroborate, centrifugation of a mixture, separation of so-formed derivative by micellar electrokinetic chromatography with sweeping and UV detection at 375 nm. A fused-silica capillary with an inlet to detector length of 51.5 cm and a total length of 60 cm (75 μm id) was served as a separation column. The separation voltage of 20.5 kV (∼160 mA) and buffer solution consisting of 0.055 mol/L sodium phosphate (pH 8), 25% acetonitrile, and 0.035 mol/L sodium dodecyl sulfate were found to be the most suitable conditions for the effective separation. The limit of quantification for thiosulfate was 4 μmol/L urine. The method was validated and calibrated for thiosulfate in the range of 4-64 μmol/L (R(2) = 0.9997). The relative standard deviation of the points of the calibration curve varied from 1.2 to 4.8% RSD.
authors.affiliation: Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Kubalczyk
authors.affiliation: Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Chwatko
authors.affiliation: Department of Environmental Chemistry, Faculty of Chemistry, University of Łódź, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Głowacki
mesh_terms: D000328:Adult; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D013885:Thiosulfates / Q000652:urine*
chemical_list: D013885:Thiosulfates
keywords: Derivatization; MEKC; Sweeping; Thiosulfate; Urine