title: Ethylene and 1-MCP regulate major volatile biosynthetic pathways in apple fruit.
abstract: The effects of ethylene and 1-methylcyclopropene (1-MCP) on apple fruit volatile biosynthesis and gene expression were investigated. Statistical analysis identified 17 genes that changed significantly in response to ethylene and 1-MCP treatments. Genes encoding branched-chain amino acid aminotransferase (BCAT), aromatic amino acid aminotransferase (ArAT) and amino acid decarboxylases (AADC) were up-regulated during ripening and further enhanced by ethylene treatment. Genes related to fatty acid synthesis and metabolism, including acyl-carrier-proteins (ACPs), malonyl-CoA:ACP transacylase (MCAT), acyl-ACP-desaturase (ACPD), lipoxygenase (LOX), hydroperoxide lyase (HPL), alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC2), β-oxidation, acyl-CoA synthetase (ACS), enoyl-CoA hydratase (ECHD), acyl-CoA dehydrogenase (ACAD), and alcohol acyltransferases (AATs) also increased during ripening and in response to ethylene treatment. Allene oxide synthase (AOS), alcohol dehydrogenase 1 (ADH1), 3-ketoacyl-CoA thiolase and branched-chain amino acid aminotransferase 2 (BCAT2) decreased in ethylene-treated fruit. Treatment with 1-MCP and ethylene generally produced opposite effects on related genes, which provides evidence that regulation of these genes is ethylene dependent.
authors.affiliation: College of Horticulture, South China Agriculture University, Guangzhou, China.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Agriculture and Agri-Food Canada, AFHRC, Kentville, Nova Scotia B4N 1J5, Canada. Electronic address: jun.song@agr.gc.ca.
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Horticulture, South China Agriculture University, Guangzhou, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Du
authors.affiliation: Agriculture and Agri-Food Canada, AFHRC, Kentville, Nova Scotia B4N 1J5, Canada.
authors.forename: Charles
authors.initials: C
authors.lastname: Forney
authors.affiliation: Agriculture and Agri-Food Canada, AFHRC, Kentville, Nova Scotia B4N 1J5, Canada.
authors.forename: Leslie
authors.initials: L
authors.lastname: Campbell-Palmer
authors.affiliation: Agriculture and Agri-Food Canada, AFHRC, Kentville, Nova Scotia B4N 1J5, Canada.
authors.forename: Sherry
authors.initials: S
authors.lastname: Fillmore
authors.affiliation: Agriculture and Agri-Food Canada, PARC, Summerland, British Columbia V0H 1Z0, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Wismer
authors.affiliation: College of Horticulture, South China Agriculture University, Guangzhou, China.
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D053898:Biosynthetic Pathways; D003521:Cyclopropanes / Q000737:chemistry*; D005030:Ethylenes / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression; D027845:Malus / Q000737:chemistry*
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D005227:Fatty Acids; C036216:ethylene; C412563:1-methylcyclopropene
keywords: Branched amino acids; Fatty acid metabolism; Fruit ripening; Gene expression; Malus domestica