title: Copper oxide nanoparticles aggravate airway inflammation and mucus production in asthmatic mice via MAPK signaling.
abstract: Copper oxide nanoparticles (CuONPs), metal oxide nanoparticles were used in multiple applications including wood preservation, antimicrobial textiles, catalysts for carbon monoxide oxidation and heat transfer fluid in machines. We investigated the effects of CuONPs on the respiratory system in Balb/c mice. In addition, to investigate the effects of CuONPs on asthma development, we used a murine model of ovalbumin (OVA)-induced asthma. CuONPs markedly increased airway hyper-responsiveness (AHR), inflammatory cell counts, proinflammatory cytokines and reactive oxygen species (ROS). CuONPs induced airway inflammation and mucus secretion with increases in phosphorylation of the MAPKs (Erk, JNK and p38). In the OVA-induced asthma model, CuONPs aggravated the increased AHR, inflammatory cell count, proinflammatory cytokines, ROS and immunoglobulin E induced by OVA exposure. In addition, CuONPs markedly increased inflammatory cell infiltration into the lung and mucus secretions, and MAPK phosphorylation was elevated compared to OVA-induced asthmatic mice. Taken together, CuONPs exhibited toxicity on the respiratory system, which was associated with the MAPK phosphorylation. In addition, CuONPs exposure aggravated the development of asthma. We conclude that CuONPs exposure has a potential toxicity in humans with respiratory disease.
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .|b College of Life Science and Biotechnology, Korea University , Seoul , Republic of Korea , and.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: c College of Veterinary Medicine, Chonnam National University , Gwangju , Republic of Korea.
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Lee
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .
authors.forename: Na-Rae
authors.initials: NR
authors.lastname: Shin
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .
authors.forename: Chan-Mi
authors.initials: CM
authors.lastname: Jeon
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.affiliation: c College of Veterinary Medicine, Chonnam National University , Gwangju , Republic of Korea.
authors.forename: Je-Won
authors.initials: JW
authors.lastname: Ko
authors.affiliation: c College of Veterinary Medicine, Chonnam National University , Gwangju , Republic of Korea.
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.affiliation: c College of Veterinary Medicine, Chonnam National University , Gwangju , Republic of Korea.
authors.forename: In-Sik
authors.initials: IS
authors.lastname: Shin
authors.affiliation: a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology , Ochang-eup, Cheongwon-gu , Chungju-si, Chungbuk , Republic of Korea .
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid; D002452:Cell Count; D003300:Copper / Q000737:chemistry / Q000633:toxicity*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000139:chemically induced*; D008168:Lung / Q000378:metabolism / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009093:Mucus / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010047:Ovalbumin; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D012130:Respiratory Hypersensitivity / Q000139:chemically induced
chemical_list: D016207:Cytokines; D017382:Reactive Oxygen Species; D007073:Immunoglobulin E; D003300:Copper; D010047:Ovalbumin; D020928:Mitogen-Activated Protein Kinases; D020780:Matrix Metalloproteinase 9; C000520:cuprous oxide
keywords: Asthma; MAPKs; copper oxide nanoparticle; respiratory system