title: Characterization and Quantification by LC-MS/MS of the Chemical Components of the Heating Products of the Flavonoids Extract in Pollen Typhae for Transformation Rule Exploration.
abstract: The Traditional Chinese Medicine herbs Pollen Typhae and Pollen Typhae Carbonisatus have been used as a hemostatic medicine promoting blood clotting for thousands of years. In this study, a reliable, highly sensitive method based on LC-MS/MS has been developed for differentiation of the heating products of total flavonoids in Pollen Typhae (FPT-N). Twenty three peaks were detected and 18 peaks have been structurally identified by comparing retention times, high resolution mass spectrometry data, and fragment ions with those of the reference substances and/or literature data. Additionally, 15 compounds have been quantified by multiple reaction monitoring in the negative ionization mode. It was found that the contents of the characterized compounds differed greatly from each other in FPT-N samples. Among them, the content of huaicarbon B significantly increased at first, while it decreased after heating for 25 min, which could be considered as the characteristic component for distinguishing FPT-N. The present study provided an approach to rapidly distinguish the differences of FPT-N samples. In addition, the actively summarized characteristic fragmentation might help deducing the structure of unknown flavonols compounds. Furthermore, transformation rules of flavonoids during the heating process in carbonisatus development could contribute to hemostatic therapeutic component exploration.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. cyqzs88@163.com.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yuhongli76@126.com.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. yuhongli76@126.com.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China. yuhongli76@126.com.
authors.forename: Hongli
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. whao5795@163.com.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China. whao5795@163.com.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210023, China. whao5795@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. pan_yaozong@126.com.
authors.forename: Yaozong
authors.initials: Y
authors.lastname: Pan
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. wjwkl@126.com.
authors.forename: Kuilong
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. yangpingctt@126.com.
authors.forename: Yangping
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. didiaodexiaojuren@163.com.
authors.forename: Chengchao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D006358:Hot Temperature; D015394:Molecular Structure; D011058:Pollen / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D032401:Typhaceae / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids
keywords: Pollen Typhae; chemical transformation; flavonoids; heating products; liquid chromatography-tandem mass spectroscopy