title: Ameliorative effect of naringin against doxorubicin-induced acute cardiac toxicity in rats.
abstract: BACKGROUND
  Doxorubicin (Dox) is one of the most active chemotherapeutic agents used to treat various types of cancers. Its clinical utility is compromised due to fatal cardiac toxicity characterized by an irreversible cardiomyopathy.
  
  
  OBJECTIVE
  This study evaluates the cardioprotective potential of naringin (NR) against Dox-induced acute cardiac toxicity in rats.
  
  
  METHODS
  Male Wistar rats were randomly divided into five groups. NR (50 and 100 mg/kg) was administered intraperitoneally (i.p.) daily from 0 to 14 d. Doxorubicin (15 mg/kg, i.p.) was given as a single dose on the 10th day. On the 14th day, all animals were sacrificed and oxidative stress parameters that include malondialdehyde (MDA), glutathione (GSH) level, superoxide dismutase (SOD), catalase (CAT) activities, and all mitochondrial complexes (I-IV) activities were evaluated along with histopathological studies of the heart.
  
  
  RESULTS
  Doxorubicin-induced cardiotoxicity was confirmed by increased (p < 0.05) MDA, decreased (p < 0.05) GSH levels, SOD, and CAT activities, mitochondrial complexes (I-IV) activities in the heart tissue. NR (100 mg/kg) showed cardioprotection as evident from significant decreased MDA (p < 0.001) level, raised (p < 0.001) GSH level, SOD and CAT activities and increased mitochondrial complexes I (p < 0.01), II (p < 0.001), III (p < 0.001), and IV (p < 0.05) activities. Further, Dox-induced cardiotoxicity was confirmed by histopathological studies. These obtained results indicated the protective role of NR against Dox-induced cardiac toxicity in rats.
  
  
  CONCLUSIONS
  NR can be used in combination with Dox due to its high cardioprotective effect against Dox-induced cardiomyopathy.
authors.affiliation: a Pharmacology Research Laboratory, Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) , New Delhi , India .
authors.forename: Mohit
authors.initials: M
authors.lastname: Kwatra
authors.affiliation: a Pharmacology Research Laboratory, Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) , New Delhi , India .
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
authors.affiliation: b Department of Pharmacology and Toxicology , National Institute of Pharmaceutical Education and Research Guwahati , Guwahati , Assam , India .
authors.forename: Ashok
authors.initials: A
authors.lastname: Jangra
authors.affiliation: c Department of Toxicology and Cancer Biology , College of Medicine, University of Kentucky , Lexington , KY , USA .
authors.forename: Murli
authors.initials: M
authors.lastname: Mishra
authors.affiliation: b Department of Pharmacology and Toxicology , National Institute of Pharmaceutical Education and Research Guwahati , Guwahati , Assam , India .
authors.forename: Sahabuddin
authors.initials: S
authors.lastname: Ahmed
authors.affiliation: d Department of Pharmacology , Bharati Vidyapeeth University, Poona College of Pharmacy , Erandwane , Pune , Maharashtra , India , and.
authors.forename: Pinaki
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: a Pharmacology Research Laboratory, Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) , New Delhi , India .
authors.forename: Divya
authors.initials: D
authors.lastname: Vohora
authors.affiliation: a Pharmacology Research Laboratory, Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University) , New Delhi , India .|e Department of Pharmacology , Gulf Medical University , Ajman , United Arab Emirates.
authors.forename: Razia
authors.initials: R
authors.lastname: Khanam
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D066126:Cardiotoxicity / Q000188:drug therapy* / Q000378:metabolism; D004317:Doxorubicin / Q000633:toxicity*; D044950:Flavanones / Q000494:pharmacology / Q000627:therapeutic use*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000903:Antibiotics, Antineoplastic; D044950:Flavanones; D004317:Doxorubicin; C005274:naringin
keywords: Mitochondrial complexes; oxidative stress; reactive oxygen species