title: Downregulation of miR-329 promotes cell invasion by regulating BRD4 and predicts poor prognosis in hepatocellular carcinoma.
abstract: Increasing evidence indicates that abnormal microRNA (miRNA) expression is related to hepatocellular carcinoma (HCC) development. Our study aimed to elucidate the essential role of miR-329 in HCC progression. Real-time PCR was used to analyze miR-329 and bromodomain containing 4 (BRD4) expression in HCC samples (n = 135). Cell Counting Kit-8 (CCK-8) and flow cytometric analysis were used to investigate cell proliferation and apoptosis. The transwell assay was used to examine the cell invasive ability. The regulation mechanism was confirmed by luciferase reporter and western blot assays. Kaplan-Meier analysis was used to detect the function of miR-329 on the prognosis of HCC patients. miR-329 was decreased in HCC samples and was related to tumor development. Furthermore, miR-329 significantly regulated cell invasion by targeting BRD4 but had no effect on cell proliferation and apoptosis. Moreover, downregulation of miR-329 predicted poor prognosis of HCC patients. miR-329 could control cell invasion via regulating BRD4 expression and may be a prognostic marker in HCC.
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of B-Ultrasound Room, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of B-Ultrasound Room, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Operating Theater, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Orthopedic, Affiliated Yixing Hospital of Jiangsu University, Yixing, 214200, People's Republic of China. staff528@yxph.com.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018797:Cell Cycle Proteins; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D011237:Predictive Value of Tests; D011379:Prognosis; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000092468:Bromodomain Containing Proteins
chemical_list: D018797:Cell Cycle Proteins; D035683:MicroRNAs; D009687:Nuclear Proteins; D014157:Transcription Factors; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins; C584396:MIRN329 microRNA, human
keywords: BRD4; HCC; Invasion; Prognosis; miR-329