title: Antibiotic Resistance Patterns and Genetic Diversity in Clinical Isolates of Pseudomonas aeruginosa Isolated From Patients of a Referral Hospital, Isfahan, Iran.
abstract: BACKGROUND
  Pseudomonas aeruginosa is a well-known opportunistic pathogen, which affects hospitalized patients in different wards due to its natural resistance to drugs.
  
  
  OBJECTIVE
  The purpose of the current study was to determine the antibiotic susceptibility profiles and genetic relatedness in P. aeruginosa isolated from patients admitted to a referral hospital in Isfahan, Iran.
  
  
  METHODS
  Out of 150 analyzed samples, 54 P. aeruginosa isolates were recovered and were subjected to antibiotic resistance patterns and genetic diversity determination by Kirby-Bauer's disk diffusion method and RAPD-PCR, respectively.
  
  
  RESULTS
  The highest percentage of resistance was observed against ceftazidime and imipenem with 30 (55.6%) isolates; meanwhile all isolates were sensitive to polymyxin B. Twenty-eight (51.8%) isolates revealed resistance to all applied antibiotics. RAPD-PCR (Random Amplified Polymorphic DNA- Polymerase Chain Reaction) results showed 54 unique genotypes, which were divided into 39 clusters.
  
  
  CONCLUSIONS
  Although different source of P. aeruginosa may involve in patient colonization, genetically related strains were isolated from different wards and or the same ward of the hospital. Our results pointed to the restriction of currently used antibiotics in studied hospital. We hope that our results cast light on the control and transmission of the infection in the investigated hospital.
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Vaez
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Faghri
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Nasr Esfahani
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Sharareh
authors.initials: S
authors.lastname: Moghim
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Fazeli
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Sedighi
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR Iran.
authors.forename: Hajieh
authors.initials: H
authors.lastname: Ghasemian Safaei
keywords: Antibiotic Resistance; Pseudomonas aeruginosa; RAPD-PCR