title: Development of a Sustainable Procedure for the Recovery of Hydroxytyrosol from Table Olive Processing Wastewater Using Adsorption Resin Technology and Centrifugal Partition Chromatography.
abstract: The present endeavor aims to establish a novel procedure, applicable to the extraction and isolation of hydroxytyrosol from table olive processing wastewater. A two-step chromatographic separation is presented using non-ionic absorbent resin for the recovery of its phenolic content, followed by purification of hydroxytyrosol with centrifugal partition chromatography. Two table olive processing wastewaters, obtained from Kalamon and Amfissis olive varieties, were used. In the extracts obtained after resin treatment, the hydroxytyrosol content was determined by high-performance liquid chromatography with diode-array detection to be 4.05% and 10.10%, respectively. The extract from Amfissis table olive processing wastewater was further processed with preparative centrifugal partition chromatography for the purification of hydroxytyrosol. High-performance liquid chromatography analysis revealed that the isolated compound was >95% purity.
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Nikos
authors.initials: N
authors.lastname: Xynos
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Dennis
authors.initials: D
authors.lastname: Abatis
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Argyropoulou
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Polychronopoulos
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Nektarios
authors.initials: N
authors.lastname: Aligiannis
authors.affiliation: Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Alexios-Leandros
authors.initials: AL
authors.lastname: Skaltsounis
mesh_terms: D000327:Adsorption; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007475:Ion Exchange Resins; D015394:Molecular Structure; D031658:Olea / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D011137:Polystyrenes; D011145:Polyvinyls; D012997:Solvents; D062065:Wastewater; D018508:Water Purification
chemical_list: D007475:Ion Exchange Resins; D010626:Phenylethyl Alcohol; D010936:Plant Extracts; D011137:Polystyrenes; D011145:Polyvinyls; D012997:Solvents; D062065:Wastewater; C005975:3,4-dihydroxyphenylethanol; C012101:XAD-4 resin