title: Postsynaptic Potentiation in Mouse Motor Synapses Induced by ATP Accumulation in Synaptic Cleft.
abstract: In mouse motor synapses, a non-selective purinoceptor antagonist suramin increased the quantum content of endplate potentials (EPP) without changing the time course of synaptic potentials. An ectonucleotidase inhibitor ARL 67156 had no effect on the amplitude and quantum content of EPP and miniature endplate potentials (mEPP) evoked by single stimuli, but significantly prolonged their duration. Long-term high-frequency stimulation of the nerve in the presence of ARL 67156 persistently increased the amplitude and duration of EPP during the train of impulses, but did not change their quantum content. ATP-γ-S, a non-hydrolyzed ATP analogue, significantly increased the amplitudes and prolonged the rising and falling phases of EPP and mEPP. The ATP-induced postsynaptic potentiation in neuromuscular transmission can result from the increase in ATP content and its longer presence in the synaptic cleft.
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia. balezina@mail.ru.
authors.forename: P O
authors.initials: PO
authors.lastname: Bogacheva
authors.affiliation: Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: O P
authors.initials: OP
authors.lastname: Balezina
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D004558:Electric Stimulation; D051379:Mice; D055369:Miniature Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D009045:Motor Endplate / Q000187:drug effects* / Q000502:physiology; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D059227:Postsynaptic Potential Summation / Q000187:drug effects* / Q000502:physiology; D058914:Purinergic Antagonists / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000378:metabolism; D013498:Suramin / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D046509:Tissue Culture Techniques
chemical_list: C092431:6-N,N-diethyl-beta,gamma-dibromomethylene-D-ATP; D058914:Purinergic Antagonists; D011983:Receptors, Purinergic; C022571:adenosine 5'-O-(3-thiotriphosphate); D013498:Suramin; D000255:Adenosine Triphosphate; D009708:Nucleotidases
keywords: ATP; ATP-γ-S; ectonucleotidase; endplate potentials; motor synapse