title: Expression of angiotensin II receptors in the caprine ovary and improvement of follicular viability in vitro.
abstract: This study aimed to evaluate mRNA levels of angiotensin II (ANG II) receptors (AGTR1 and AGTR2) in caprine follicles and to investigate the influence of ANG II on the viability and in vitro growth of preantral follicles. Real-time polymerase chain reaction (PCR) was used to quantify AGTR1 and AGTR2 mRNA levels in the different follicular stages. For culture, caprine ovaries were collected, cut into 13 fragments and then either directly fixed for histological and ultrastructural analysis (fresh control) or placed in culture for 1 or 7 days in α-minumum essential medium plus (α-MEM+) with 0, 1, 5, 10, 50 or 100 ng/ml ANG II. Then, the fragments were destined to morphological, viability and ultrastructural analysis. The results showed that primordial follicles had higher levels of AGTR1 and AGTR2 mRNA than secondary follicles. Granulosa/theca cells from antral follicles had higher levels of AGTR1 mRNA than their respective cumulus-oocyte complex (COCs). After 7 days of culture, ANG II (10 or 50 ng/ml) maintained the percentages of normal follicles compared with α-MEM+. Fluorescence and ultrastructural microscopy confirmed follicular integrity in ANG II (10 ng/ml). In conclusion, a high expression of AGTR1 and AGTR2 is observed in primordial follicles. Granulosa/theca cells from antral follicles had higher levels of AGTR1 mRNA. Finally, 10 ng/ml ANG II maintained the viability of caprine preantral follicles after in vitro culture.
authors.affiliation: Programa de Pós-Graduação em Ciências Veterinárias (PPGCV).Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA).Universidade Estadual do Ceará (UECE).Av. Paranjana,1700,Campus do Itaperi. Fortaleza,CEBrasilCEP 60740 903.
authors.forename: J B
authors.initials: JB
authors.lastname: Bruno
authors.affiliation: Institute for Technology and Research,Tiradentes University,Aracaju-SE,Brazil.
authors.forename: I B
authors.initials: IB
authors.lastname: Lima-Verde
authors.affiliation: Institute of Health Sciences,University of International Integration Lusophone African-Brazilian,Acarape-CE,Brazil.
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.affiliation: Faculty of Veterinary Medicine,LAMOFOPA,PPGCV,State University of Ceara,Fortaleza-CE,Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.affiliation: Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of São Francisco Valley,Petrolina-PE,Brazil.
authors.forename: M H T
authors.initials: MH
authors.lastname: Matos
authors.affiliation: Faculty of Veterinary Medicine,LAMOFOPA,PPGCV,State University of Ceara,Fortaleza-CE,Brazil.
authors.forename: L R
authors.initials: LR
authors.lastname: Faustino
authors.affiliation: Laboratory of Ultrastructure, CPqAM/Fiocruz,Federal University of Pernambuco,Recife-PE,Brazil.
authors.forename: M A M
authors.initials: MA
authors.lastname: Donato
authors.affiliation: Laboratory of Ultrastructure, CPqAM/Fiocruz,Federal University of Pernambuco,Recife-PE,Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Peixoto
authors.affiliation: Faculty of Veterinary Medicine,LAMOFOPA,PPGCV,State University of Ceara,Fortaleza-CE,Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Biotechnology Nucleus of Sobral (NUBIS),Federal University of Ceara,Sobral-CE,Brazil.
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.affiliation: Faculty of Veterinary Medicine,LAMOFOPA,PPGCV,State University of Ceara,Fortaleza-CE,Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002470:Cell Survival / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D006041:Goats; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism* / Q000648:ultrastructure; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D014662:Vasoconstrictor Agents / Q000494:pharmacology
chemical_list: D020033:Protein Isoforms; D011945:Receptors, Angiotensin; D014662:Vasoconstrictor Agents; D000804:Angiotensin II
keywords: Angiotensin II; Caprine; Culture; Ovary; Preantral follicles