title: Analysis of Salmonella PhoP/PhoQ regulation by dimethyl-SRM-based quantitative proteomics.
abstract: SRM (selected reaction monitoring), a tandem mass spectrometry-based method characterized by high repeatability and accuracy, is an effective tool for the quantification of predetermined proteins. In this study, we built a time-scheduled dimethyl-SRM method that can provide the precise relative quantification of 92 proteins in one run. By applying this method to the Salmonella PhoP/PhoQ two-component system, we found that the expression of selected PhoP/PhoQ-activated proteins in response to Mg(2+) concentrations could be divided into two distinct patterns. For the time-course SRM experiment, we found that the dynamics of the selected PhoP/PhoQ-activated proteins could be divided into three distinct patterns, providing a new clue regarding PhoP/PhoQ activation and regulation. Moreover, the results for iron homeostasis proteins in response to Mg(2+) concentrations revealed that the PhoP/PhoQ two-component system may serve as a repressor for iron uptake proteins. And ribosomal protein levels clearly showed a response to different Mg(2+) concentrations and to time.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Mao-Tian
authors.initials: MT
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Qiang-Qiang
authors.initials: QQ
authors.lastname: Han
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Hao-Long
authors.initials: HL
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China. Electronic address: guol@whu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015153:Blotting, Western; D019295:Computational Biology / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D008274:Magnesium / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism; D060066:Protein Interaction Maps / Q000187:drug effects; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012475:Salmonella / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; C079792:PhoQ protein, Bacteria; D020543:Proteome; C061064:PhoP protein, Bacteria; D008274:Magnesium
keywords: Mg(2+) concentrations; SRM; Salmonella PhoP/PhoQ; Time course