title: Determination of endocrine disruptors and endogenic androgens and estrogens in rat serum by high-performance liquid chromatography-tandem mass spectrometry.
abstract: To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to extract and concentrate the analytes: bisphenol A, atrazine, vinclozolin metabolite, testosterone, androstenedione, estrone, estradiol, estrone-sulfate and glucuronide and estradiol-sulfate and glucuronide. The analyses were then performed by high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray ionisation in positive and negative modes. The protocol based on SPE was validated using the ICH/2005 guidelines. The validation demonstrated good performance in terms of linearity (R(2)>0.99), recovery (71-90%) and repeatability (relative standard deviation: 1-18%). The method was sensitive with LOQ comprised between 0.1 and 0.4 ng/ml for androgens and between 0.098 and 10.2 ng/ml for estrogens. The results obtained on the serum of rats exposed to the targeted endocrine disruptors showed the suitability of this analytical strategy.
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: M
authors.initials: M
authors.lastname: Tournier
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: C
authors.initials: C
authors.lastname: Pouech
authors.affiliation: Experimental Toxicology Unit, INERIS, Parc Technologique ALATA, F-60550 Verneuil-en-Halatte, France.
authors.forename: N
authors.initials: N
authors.lastname: Quignot
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: F
authors.initials: F
authors.lastname: Lafay
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: L
authors.initials: L
authors.lastname: Wiest
authors.affiliation: Experimental Toxicology Unit, INERIS, Parc Technologique ALATA, F-60550 Verneuil-en-Halatte, France.
authors.forename: E
authors.initials: E
authors.lastname: Lemazurier
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France.
authors.forename: C
authors.initials: C
authors.lastname: Cren-Olivé
authors.affiliation: Université de Lyon, Institut des Sciences Analytiques, UMR5280-CNRS, Université de Lyon, Université Lyon 1, ENS-Lyon, 5 rue de la Doua, 69100 Villeurbanne, France. Electronic address: emmanuelle.vulliet@isa-lyon.fr.
authors.forename: E
authors.initials: E
authors.lastname: Vulliet
mesh_terms: D000728:Androgens / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D052244:Endocrine Disruptors / Q000097:blood*; D004967:Estrogens / Q000097:blood*; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D000728:Androgens; D052244:Endocrine Disruptors; D004967:Estrogens
keywords: Endocrine disruptors; HPLC–MS/MS; Rat serum; Solid-phase extraction; Steroids; Sulfate and glucuronide conjugates