title: Collagen-containing scaffolds enhance attachment and proliferation of non-cultured bone marrow multipotential stromal cells.
abstract: Large bone defects are ideally treated with autografts, which have many limitations. Therefore, osteoconductive scaffolds loaded with autologous bone marrow (BM) aspirate are increasingly used as alternatives. The purpose of this study was to compare the growth of multipotential stromal cells (MSCs) from unprocessed BM on a collagen-containing bovine bone scaffold (Orthoss(®) Collagen) with a non-collagen-containing bovine bone scaffold, Orthoss(®) . Another collagen-containing synthetic scaffold, Vitoss(®) was included in the comparison. Colonization of scaffolds by BM MSCs (n = 23 donors) was evaluated using microscopy, colony forming unit-fibroblast assay and flow-cytometry. The number of BM MSCs initially attached to Orthoss(®) Collagen and Vitoss(®) was similar but greater than Orthoss(®) (p = 0.001 and p = 0.041, respectively). Furthermore, the number of MSCs released from Orthoss(®) Collagen and Vitoss(®) after 2-week culture was also higher compared to Orthoss(®) (p = 0.010 and p = 0.023, respectively). Interestingly, collagen-containing scaffolds accommodated larger numbers of lymphocytic and myelomonocytic cells. Additionally, the proliferation of culture-expanded MSCs on Orthoss(®) collagen and Vitoss(®) was greater compared to Orthoss(®) (p = 0.047 and p = 0.004, respectively). Collectively, collagen-containing scaffolds were superior in supporting the attachment and proliferation of MSCs when they were loaded with unprocessed BM aspirates. This highlights the benefit of collagen incorporation into bone scaffolds for use with autologous bone marrow aspirates as autograft substitutes.
authors.affiliation: Academic Unit of Musculoskeletal Disease, Leeds Institute of Rheumatic and Musculoskeletal Medicine, St. James University Hospital, University of Leeds, LS9 7TF, Leeds, United Kingdom.|NIHR, Leeds Biomedical Research Unit, Chapel Allerton Hospital, University of Leeds, LS7 4SA, Leeds, United Kingdom.
authors.forename: Jehan J
authors.initials: JJ
authors.lastname: El-Jawhari
authors.affiliation: Academic Unit of Musculoskeletal Disease, Leeds Institute of Rheumatic and Musculoskeletal Medicine, St. James University Hospital, University of Leeds, LS9 7TF, Leeds, United Kingdom.
authors.forename: Clara
authors.initials: C
authors.lastname: Sanjurjo-Rodríguez
authors.affiliation: Academic Unit of Musculoskeletal Disease, Leeds Institute of Rheumatic and Musculoskeletal Medicine, St. James University Hospital, University of Leeds, LS9 7TF, Leeds, United Kingdom.|NIHR, Leeds Biomedical Research Unit, Chapel Allerton Hospital, University of Leeds, LS7 4SA, Leeds, United Kingdom.
authors.forename: Elena
authors.initials: E
authors.lastname: Jones
authors.affiliation: Academic Unit of Musculoskeletal Disease, Leeds Institute of Rheumatic and Musculoskeletal Medicine, St. James University Hospital, University of Leeds, LS9 7TF, Leeds, United Kingdom.|NIHR, Leeds Biomedical Research Unit, Chapel Allerton Hospital, University of Leeds, LS7 4SA, Leeds, United Kingdom.
authors.forename: Peter V
authors.initials: PV
authors.lastname: Giannoudis
mesh_terms: D064591:Allografts; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D003094:Collagen; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D054457:Tissue Scaffolds
chemical_list: D003094:Collagen
keywords: bone graft substitutes; bone marrow (BM); collagen; multipotential stromal cells (MSCs); scaffolds