title: Physico-chemical characterization of asolectin-genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability.
abstract: In this study, the interaction between soy isoflavone genistein and asolectin liposomes was investigated by monitoring the effects of isoflavone on lipidic hydration, mobility, location and order. These properties were analyzed by the following techniques: horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), low-field (1)H nuclear magnetic resonance (NMR), high-field (31)P NMR, zeta potential, differential scanning calorimetry (DSC) and UV-vis spectroscopy. The antioxidant and antitumoral activities of the genistein liposomal system were also studied. The genistein saturation concentration in ASO liposomes corresponded to 484 μM. HATR-FTIR results indicated that genistein influences the dynamics of the lipidic phosphate, choline, carbonyl and acyl chain methylenes groups. At the lipid polar head, HATR-FTIR and (31)P NMR results showed that the isoflavone reduces the hydration degree of the phosphate group, as well as its mobility. Genistein ordered the lipid interfacial carbonyl group, as evidenced by the HATR-FTIR bandwidth analysis. This ordering effect was also observed in the lipidic hydrophobic region, by HATR-FTIR, NMR, DSC and turbidity responses. At the saturation concentration, liposome-loaded genistein inhibits the lipid peroxidation induced by hydroxyl radical in 90.9%. ASO liposome-loaded genistein at 100 μM decreased C6 glioma cell viability by 57% after 72 h of treatment. Results showed an increase of the genistein in vitro activities after its incorporation in liposomes. The data described in this work will contribute to a better understanding of the interaction between genistein and a natural-source membrane and of its influence on isoflavone biological activities. Furthermore, the antitumoral results showed that genistein-based liposomes, which contain natural-sourced lipids, may be promising as a drug delivery system to be used in the glioma therapy.
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos.
authors.forename: Carla Roberta
authors.initials: CR
authors.lastname: Lopes de Azambuja
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos.
authors.forename: Lurdiane Gomes
authors.initials: LG
authors.lastname: dos Santos
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos.
authors.forename: Marisa Raquel
authors.initials: MR
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos.
authors.forename: Renan Ferreira Meneses
authors.initials: RF
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal Comparada; Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Campus Universitário, 96010-900 Pelotas, RS, Brazil.
authors.forename: Elita Ferreira
authors.initials: EF
authors.lastname: da Silveira
authors.affiliation: Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Campus Universitário, 96010-900 Pelotas, RS, Brazil.
authors.forename: Juliana Hofstatter
authors.initials: JH
authors.lastname: Azambuja
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos.
authors.forename: Alex F C
authors.initials: AF
authors.lastname: Flores
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal Comparada.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Horn
authors.affiliation: Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal do Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande, RS 96203-900, Brazil.
authors.forename: Cristiana Lima
authors.initials: CL
authors.lastname: Dora
authors.affiliation: Programa de Pós-Graduação em Ciências Fisiológicas, Fisiologia Animal Comparada; Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal do Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande, RS 96203-900, Brazil.
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Muccillo-Baisch
authors.affiliation: Departamento de Ciências Básicas da Saúde (DCBS), Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.affiliation: Centro de Biotecnologia, Universidade Federal de Pelotas, Campus Universitário, 96010-900 Pelotas, RS, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: da Silva Pinto
authors.affiliation: Departamento de Química, Universidade Federal de Santa Catarina, 88040-900 Trindade, Florianópolis, SC, Brazil.
authors.forename: Alexandre Luís
authors.initials: AL
authors.lastname: Parize
authors.affiliation: Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos. Electronic address: vrlima23@hotmail.com.
authors.forename: Vânia Rodrigues
authors.initials: VR
authors.lastname: de Lima
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019833:Genistein / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005910:Glioma / Q000188:drug therapy; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D008081:Liposomes; D010713:Phosphatidylcholines; C024990:asolectin; D019833:Genistein
keywords: Asolectin liposomes; DSC; Genistein; NMR; Spectroscopy study