title: Isolation and characterization of bacteriophage SPI1, which infects the activated-sludge-foaming bacterium Skermania piniformis.
abstract: Foaming in activated sludge plants is a worldwide problem commonly caused by proliferation of bacteria of the order Corynebacteriales. These include Skermania piniformis, a filamentous bacterium that has been documented to be a major cause of foaming globally, and particularly in Australian treatment plants. Phage SPI1 is the first phage that was isolated and shown to infect this organism. It targets seven of the nine strains of S. piniformis held in our culture collection, but none of the other 73 mycolata strains of different genera, mostly isolated from wastewater, against which it was tested. Phage SPI1 is a member of the family Siphoviridae and has a circularly permuted dsDNA genome of 55,748 bp with a G+C content of 67.8 mol %. It appears to be obligatorily lytic, with no evidence of genes related to a lysogenic mode of existence.
authors.affiliation: La Trobe Institute of Molecular Sciences, Bendigo, VIC, 3550, Australia.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Dyson
authors.affiliation: La Trobe Institute of Molecular Sciences, Bendigo, VIC, 3550, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Tucci
authors.affiliation: Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, 3086, Australia.
authors.forename: R J
authors.initials: RJ
authors.lastname: Seviour
authors.affiliation: Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, VIC, 3086, Australia. steve.petrovski@latrobe.edu.au.
authors.forename: S
authors.initials: S
authors.lastname: Petrovski
mesh_terms: D000192:Actinomycetales / Q000821:virology*; D001315:Australia; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*
chemical_list: D012722:Sewage
keywords: Activated sludge foaming; Bacteriophage; Bio-control; PTLO; Phage; Phage therapy; Skermania piniformis; Wgs