title: Expression and clinical significance of long non-coding RNA HNF1A-AS1 in human gastric cancer.
abstract: BACKGROUND
  Increasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in the occurrence and development of human cancers, including gastric cancer (GC). However, the functional and clinical significance of lncRNAs are still poorly understood.
  
  
  METHODS
  In this study, the expression of LncRNA HNF1A antisense RNA 1 (HNF1A-AS1) was first examined by lncRNAs microarray analysis in 6 GC tissues, and was then further verified by real-time quantitative reverse transcription PCR (qRT-PCR) both in 3 GC cell lines and 161 cases of GC tissues. We also evaluated the association between HNF1A-AS1 expression and clinicopathological features of patients with GC.
  
  
  RESULTS
  LncRNAs microarray analysis results exhibited that HNF1A-AS1 was downregulated in GCs tissues (mean fold change 2.06, p < 0.05), which was further confirmed by qRT-PCR. The results from qRT-PCR showed that the expression of HNF1A-AS1 was not only downregulated in three GC cell lines (AGS, BGC-823, and MKN-45) relative to that in a normal gastric mucosal epithelial cell line (GES-1), but also decreased in GC tissues relative to that in paired adjacent non-neoplastic tissues (low expression, 94 of 161; low expression rate, 58.38%). Furthermore, low HNF1A-AS1 expression was associated with tumor size/diameter (p = 0.005, multivariate analysis), levels of serum carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9), and RRM1 expression in tissue samples (p = 0.028, p = 0.009, and p = 0.006, respectively).
  
  
  CONCLUSIONS
  Taken together, our data indicate that lncRNA HNF1A-AS1 may be a regulator of GC, and thus, it may have potential as a novel biomarker and treatment target for this type of cancer.
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. dangyuan830307@hotmail.com.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. fhlan@mail.fjmu.edu.cn.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Lan
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. 798951352@qq.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. posuly@sina.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. 2444734512@qq.com.
authors.forename: Youdong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Pathology, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. yuyinghao0808@126.com.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. fzptwk@xmu.edu.cn.
authors.forename: Lie
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. flyfishwang@hotmail.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Experimental Medicine, Fuzhou General Hospital (Dongfang Hospital), 156 North Xi-er Huan Road, Fuzhou City, Fujian Province, 350025, China. huangqj100@126.com.
authors.forename: Qiaojia
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005500:Follow-Up Studies; D005753:Gastric Mucosa / Q000378:metabolism*; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000037:antagonists & inhibitors* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C496487:HNF1A protein, human; D051538:Hepatocyte Nuclear Factor 1-alpha; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D034741:RNA, Small Interfering