title: An aqueous platinum nanotube based fluorescent immuno-assay for porcine reproductive and respiratory syndrome virus detection.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) has been a significant pathogen towards global swine industry upon its emergence in the late 1980s and since then has exemplified a rapidly evolving, widely spreading pathogen. It is urgently important to develop a simple, rapid and cost effective method to detect this pathogen when virus outbreaks. In the present work, it was found that virus antibody modified platinum nanotubes (Pt-Ab) could act as a superquencher to CdTe:Zn(2+) quantum dots (CdTe:Zn(2+) QDs) fluorescence by Stern-Volmer constants nearly 10(9) M(-1) without any aggregation, the CdTe:Zn(2+) QDs fluorescence will recover as the Pt-Ab goes away by antibody and antigen interaction when virus was added into the probe solution, releasing CdTe:Zn(2+) QDs from the surface of Pt-Ab. By the recovery fluorescence intensity, it can realize qualitative and quantitative detection of PRRSV. This method gives a fast response to PRRSV concentration and provides a sensitive detection limit (2.4 ng/mL). Moreover, it can be applied in infected porcine serum samples and obtain satisfied results.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Cai
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: hyhan@mail.hzau.edu.cn.
authors.forename: Heyou
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry; D005240:Feasibility Studies; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D043942:Nanotubes / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000302:isolation & purification*; D045663:Quantum Dots / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013552:Swine; D013691:Tellurium / Q000737:chemistry; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
chemical_list: D000914:Antibodies, Viral; D019187:Cadmium Compounds; D014867:Water; D010984:Platinum; D015032:Zinc; D013691:Tellurium; C028337:cadmium telluride
keywords: Fluorescence; PRRSV detection; PtNTs; Quantum dot