title: Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody.
abstract: We report the crystal structure of the M2 ectodomain (M2e) in complex with a monoclonal antibody that binds the amino terminus of M2. M2e extends into the antibody binding site to form an N-terminal β-turn near the bottom of the paratope. This M2e folding differs significantly from that of M2e in complex with an antibody that binds another part of M2e. This suggests that M2e can adopt at least two conformations that can elicit protective antibodies.
authors.affiliation: Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Ki Joon
authors.initials: KJ
authors.lastname: Cho
authors.affiliation: Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Bert
authors.initials: B
authors.lastname: Schepens
authors.affiliation: Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Kristof
authors.initials: K
authors.lastname: Moonens
authors.affiliation: Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Lei
authors.initials: L
authors.lastname: Deng
authors.affiliation: Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
authors.forename: Walter
authors.initials: W
authors.lastname: Fiers
authors.affiliation: Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Han
authors.initials: H
authors.lastname: Remaut
authors.affiliation: Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium xavier.saelens@vib-ugent.be.
authors.forename: Xavier
authors.initials: X
authors.lastname: Saelens
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000914:Antibodies, Viral / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D018360:Crystallography, X-Ray; D006801:Humans; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D011487:Protein Conformation; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D000086082:Viroporin Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins