title: Effect of Sulfated Polysaccharides from Brown Algae on Apoptosis of Human Peripheral Blood Lymphocytes.
abstract: We studied the influence of fucoidans from brown algae Fucus evanescens, Laminaria cichorioides and Laminaria japonica on apoptosis of human peripheral blood lymphocytes. It was demonstrated that fucoidans induced lymphocyte apoptosis, increased the proportion of cells with low mitochondrial transmembrane potential, and inhibit expression of Bcl-xL gene in blood lymphocytes. These findings suggest that lymphocyte apoptosis induced by the analyzed sulfated polysaccharides is mediated by mitochondrial pathway.
authors.affiliation: G. P. Somov Research Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Sciences, Vladivostok, Russia. angazha@mail.ru.
authors.forename: A K
authors.initials: AK
authors.lastname: Gazha
authors.affiliation: G. P. Somov Research Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: T S
authors.initials: TS
authors.lastname: Zaporozhets
authors.affiliation: G. P. Somov Research Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Kuznetsova
authors.affiliation: G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Zvyaguintseva
authors.affiliation: G. P. Somov Research Institute of Epidemiology and Microbiology, Siberian Division of the Russian Academy of Sciences, Vladivostok, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Besednova
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D044645:Fucus / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007795:Laminaria / Q000737:chemistry*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D061251:Primary Cell Culture; D013431:Sulfates; D051020:bcl-X Protein / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: C494811:BCL2L1 protein, human; D011134:Polysaccharides; D013431:Sulfates; D051020:bcl-X Protein
keywords: apoptosis; fucoidan; mitochondrial membrane potential; periperal blood lymphocytes