title: Effects of nanocrystalline PbSe on hematopoietic system and bone marrow micronucleus rate of rats.
abstract: OBJECTIVE
  To investigate the effect of lead selenide nanocrystals on hematopoietic system and bone marrow micronucleus rate of rats.
  
  
  METHODS
  Specific pathogen free SD rats were randomly divided into 4 groups (8 rats in each group), and injected with of 0 (control group), 10 (low dose group), 20 (middle dose group), 30 mg/kg (high dose group) nanocrystalline PbSe, respectively. Seven weeks after injection, the blood was taken from rats for routine index detection; the number of micronucleus cells per 1000 polychromatic erythrocyte from bone marrow was counted.
  
  
  RESULTS
  White blood cell (WBC), lymphocyte (LYM) count in low dose group rats, and WBC, LYM, granulocyte (GRN), monocytes (MOD) counts in high dose group significantly increased compared to those of control group. LYM% ratio decreased while GRN% ratio increased along with the increase of exposure dosage. Compared with those of the control group, levels of erythrocyte mean corpuscular volume (MCV) in low dose group, hemoglobin (HGB), red blood cell specific volume (HCT), MCV in middle dose group and red blood cell (RBC), HGB, HCT, MCV in high dose group, were markedly decreased. Red blood cell distribution width (RDW), blood platelet (PLT) levels in three exposure groups of were higher than those in control group. Marrow micronucleus test results showed that, the micronucleus rate rise in mid dose and high dose group compared with the control group, suggesting that nanocrystalline PbSe has genetic toxicity on rats.
  
  
  CONCLUSIONS
  Nano PbSe can lead to changes in blood routine index and bone marrow micronucleus rate, and its toxicity was positively related to the dosage.
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liang
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
authors.forename: Qingzhao
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
authors.forename: Zongkai
authors.initials: Z
authors.lastname: Yue
authors.affiliation: School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China. Electronic address: zhengying0918@aliyun.com.
authors.forename: Guoying
authors.initials: G
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006413:Hematopoietic System / Q000187:drug effects*; D007267:Injections; D007854:Lead / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D015162:Micronucleus Tests; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D018036:Selenium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*
chemical_list: D018036:Selenium Compounds; C088065:lead selenide; D007854:Lead
keywords: Blood system; Bone marrow micronucleus rate; Nanocrystalline PbSe; Rat