title: Current status and recent advantages in derivatization procedures in human doping control.
abstract: Derivatization is one of the most important steps during sample preparation in doping control analysis. Its main purpose is the enhancement of chromatographic separation and mass spectrometric detection of analytes in the full range of laboratory doping control activities. Its application is shown to broaden the detectable range of compounds, even in LC-MS analysis, where derivatization is not a prerequisite. The impact of derivatization initiates from the stage of the metabolic studies of doping agents up to the discovery of doping markers, by inclusion of the screening and confirmation procedures of prohibited substances in athlete's urine samples. Derivatization renders an unlimited number of opportunities to advanced analyte detection.
authors.affiliation: Laboratory of Biopharmaceutics & Pharmacokinetics, Faculty of Pharmacy, National & Kapodistrian University of Athens, Greece.|Anti-Doping Lab Qatar, Sports City Road, Aspire Zone, P.O. Box 27775, Doha, Qatar.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Athanasiadou
authors.affiliation: Doping Control Laboratory of Athens, Olympic Athletic Center of Athens (OAKA), 15123 Maroussi, Greece.
authors.forename: Polyxeni
authors.initials: P
authors.lastname: Kiousi
authors.affiliation: Doping Control Laboratory of Athens, Olympic Athletic Center of Athens (OAKA), 15123 Maroussi, Greece.
authors.forename: Nasia
authors.initials: N
authors.lastname: Kioukia-Fougia
authors.affiliation: Doping Control Laboratory of Athens, Olympic Athletic Center of Athens (OAKA), 15123 Maroussi, Greece.
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Lyris
authors.affiliation: Doping Control Laboratory of Athens, Olympic Athletic Center of Athens (OAKA), 15123 Maroussi, Greece.
authors.forename: Yiannis S
authors.initials: YS
authors.lastname: Angelis
mesh_terms: D000081:Acetamides / Q000737:chemistry; D058666:Adrenergic beta-2 Receptor Agonists / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004300:Doping in Sports; D005463:Fluoroacetates / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D059627:Liquid Phase Microextraction; D052616:Solid Phase Extraction; D013256:Steroids / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine*; D053719:Tandem Mass Spectrometry; D014297:Trimethylsilyl Compounds / Q000737:chemistry
chemical_list: D000081:Acetamides; D058666:Adrenergic beta-2 Receptor Agonists; D005463:Fluoroacetates; D013256:Steroids; D014297:Trimethylsilyl Compounds; C086665:N-methyl-N-(trimethylsilyl)trifluoroacetamide