title: Overexpression of Three Glucosinolate Biosynthesis Genes in Brassica napus Identifies Enhanced Resistance to Sclerotinia sclerotiorum and Botrytis cinerea.
abstract: Sclerotinia sclerotiorum and Botrytis cinerea are notorious plant pathogenic fungi with an extensive host range including Brassica crops. Glucosinolates (GSLs) are an important group of secondary metabolites characteristic of the Brassicales order, whose degradation products are proving to be increasingly important in plant protection. Enhancing the defense effect of GSL and their associated degradation products is an attractive strategy to strengthen the resistance of plants by transgenic approaches. We generated the lines of Brassica napus with three biosynthesis genes involved in GSL metabolic pathway (BnMAM1, BnCYP83A1 and BnUGT74B1), respectively. We then measured the foliar GSLs of each transgenic lines and inoculated them with S. sclerotiorum and B. cinerea. Compared with the wild type control, over-expressing BnUGT74B1 in B. napus increased the aliphatic and indolic GSL levels by 1.7 and 1.5 folds in leaves respectively; while over-expressing BnMAM1 or BnCYP83A1 resulted in an approximate 1.5-fold higher only in the aliphatic GSL level in leaves. The results of plant inoculation demonstrated that BnUGT74B1-overexpressing lines showed less severe disease symptoms and tissue damage compared with the wild type control, but BnMAM1 or BnCYP83A1-overexpressing lines showed no significant difference in comparison to the controls. These results suggest that the resistance to S. sclerotiorum and B. cinerea in B. napus could be enhanced through tailoring the GSL profiles by transgenic approaches or molecular breeding, which provides useful information to assist plant breeders to design improved breeding strategies.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Department of Plant Sciences, University of California Davis, One Shield Avenue, Davis, CA 95616, United States of America.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dongxin
authors.initials: D
authors.lastname: Huai
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Plant Sciences, University of California Davis, One Shield Avenue, Davis, CA 95616, United States of America; DynaMo Center of Excellence, Copenhagen Plant Science Centre, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kliebenstein
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D001203:Ascomycota / Q000472:pathogenicity; D020171:Botrytis / Q000472:pathogenicity; D029688:Brassica napus / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D005961:Glucosinolates / Q000378:metabolism; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000235:genetics; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis* / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D030821:Plants, Genetically Modified
chemical_list: D029681:Arabidopsis Proteins; D005961:Glucosinolates; D003577:Cytochrome P-450 Enzyme System; C094565:CYP83A1 protein, Arabidopsis; D005964:Glucosyltransferases; C499436:UDP-glucose thiohydroximate S-glucosyltransferase UGT74B1, Arabidopsis; D007652:Oxo-Acid-Lyases; C446529:methylthioalkylmalate synthase 1