title: A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells.
abstract: Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, a crucial epigenetic modifier, is deregulated in cancer and subjected to a variety of post-translational modifications. Here, we describe the generation of a new monoclonal antibody that specifically recognizes a novel highly dynamic prophase phosphorylation of serine 406-HDAC1, providing a powerful tool for detecting early mitotic cells.
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|b Present addresses: Fondazione Umberto Veronesi ; Piazza Velasca 5; 20121 Milano , Italy.|i These authors equally contributed to the work.
authors.forename: Chiara V
authors.initials: CV
authors.lastname: Segré
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|c Department of Chemistry and Biochemistry ; University of California; Los Angeles ; Los Angeles , CA 90095 , USA.|i These authors equally contributed to the work.
authors.forename: Silvia
authors.initials: S
authors.lastname: Senese
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|i These authors equally contributed to the work.
authors.forename: Sara
authors.initials: S
authors.lastname: Loponte
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|d Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology ; Cambridge , USA.
authors.forename: Stefano
authors.initials: S
authors.lastname: Santaguida
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|e IFOM; the FIRC Institute for Molecular Oncology Foundation ; via Adamello 16; 20139 Milan , Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Soffientini
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.|f Chromatography and Mass Spectrometry Division; Thermo Fisher Scientific ; 1400 Northpoint Parkway; Suite 10; West Palm Beach ; Fl 33407 , USA.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Grigorean
authors.affiliation: g Consortium for Genomic Technologies - COGENTECH ; via Adamello 16; 20139 Milan , Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Cinquanta
authors.affiliation: g Consortium for Genomic Technologies - COGENTECH ; via Adamello 16; 20139 Milan , Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ossolengo
authors.affiliation: h Max F. Perutz Laboratories; Medical University of Vienna ; Vienna Biocenter , Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Seiser
authors.affiliation: a Department of Experimental Oncology ; European Institute of Oncology ; Via Adamello 16; 20139 Milan , Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Chiocca
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D056284:Histone Deacetylase 1 / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011418:Prophase
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D010750:Phosphoproteins; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1
keywords: HDAC1; HDAC2; HDACs; antibody; cell cycle; checkpoint; mitosis; phosphorylation; post-translational modifications; prophase