title: Multi-copy venom genes hidden in de novo transcriptome assemblies, a cautionary tale with the snakelocks sea anemone Anemonia sulcata (Pennant, 1977).
abstract: Using a partial transcriptome of the snakelocks anemone (Anemonia sulcata) we identify toxin gene candidates that were incorrectly assembled into several Trinity components. Our approach recovers hidden diversity found within some toxin gene families that would otherwise go undetected when using Trinity, a widely used program for venom-focused transcriptome reconstructions. Unidentified hidden transcripts may significantly impact conclusions made regarding venom composition (or other multi-copy conserved genes) when using Trinity or other de novo assembly programs.
authors.affiliation: The Ohio State University, Department of Evolution, Ecology, and Organismal Biology, 1315 Kinnear Road, Columbus, OH 43215, USA. Electronic address: jason507@gmail.com.
authors.forename: Jason
authors.initials: J
authors.lastname: Macrander
authors.affiliation: The Ohio State University, Department of Evolution, Ecology, and Organismal Biology, 1315 Kinnear Road, Columbus, OH 43215, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Broe
authors.affiliation: The Ohio State University, Department of Evolution, Ecology, and Organismal Biology, 1315 Kinnear Road, Columbus, OH 43215, USA.
authors.forename: Marymegan
authors.initials: M
authors.lastname: Daly
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018628:Gene Dosage; D020869:Gene Expression Profiling / Q000379:methods*; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D016013:Likelihood Functions; D008387:Marine Toxins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012615:Sea Anemones / Q000235:genetics*; D016415:Sequence Alignment; D012984:Software
chemical_list: D008387:Marine Toxins
keywords: Next generation sequencing; Sea anemone venom; Sodium channel toxins; Trinity