title: Immunomodulatory Effects of Psyllium Extract on Helicobacter pylori Interaction With Gastric Epithelial Cells.
abstract: Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psyllium husk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori Human gastric adenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P < .001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori-stimulated IL-8 was also (P < .001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P < .0001) cagA-positive H pylori-induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P = .0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent.
authors.affiliation: Aga Khan University, Karachi, Pakistan javed.yakoob@aku.edu.
authors.forename: Javed
authors.initials: J
authors.lastname: Yakoob
authors.affiliation: Aga Khan University, Karachi, Pakistan.
authors.forename: Wasim
authors.initials: W
authors.lastname: Jafri
authors.affiliation: Aga Khan University, Karachi, Pakistan.
authors.forename: Malik Hassan
authors.initials: MH
authors.lastname: Mehmood
authors.affiliation: Aga Khan University, Karachi, Pakistan.
authors.forename: Zaigham
authors.initials: Z
authors.lastname: Abbas
authors.affiliation: Aga Khan University, Karachi, Pakistan.
authors.forename: Kanwal
authors.initials: K
authors.lastname: Tariq
mesh_terms: D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects*; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011620:Psyllium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D016328:NF-kappa B; D011620:Psyllium
keywords: Helicobacter pylori; NF-κB; gastric epithelial cells; interleukin-8; psyllium