title: Glutathione S-Transferase Gene Polymorphisms: Modulator of Genetic Damage in Gasoline Pump Workers.
abstract: This study investigated genetic damage in gasoline pump workers using the cytokinesis blocked micronucleus (CBMN) assay. Blood and urine samples were collected from 50 gasoline pump workers and 50 control participants matched with respect to age and other confounding factors except for exposure to benzene through gasoline vapors. To determine the benzene exposure, phenol was analyzed in urinary samples of exposed and control participants. Urinary mean phenol level was found to be significantly high (P < 0.05) in exposed workers. The CBMN frequency was found to be significantly higher in gasoline pump workers (6.70 ± 1.78) when compared to control individuals (2.20 ± 0.63; P < 0.05). We also investigated influence of polymorphisms of GSTM1, GSTT1, and GSTP1 genes on CBMN frequency. The individuals having GSTM1 and GSTT1 null genotypes had significantly higher frequency of CBMN (P < 0.05). Our study indicates that chronic and long-term exposure of gasoline vapors can increase genotoxic risk in gasoline pump workers.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Kanu
authors.initials: K
authors.lastname: Priya
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India ayadav@kuk.ac.in.
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Gulati
authors.affiliation: Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Aggarwal
authors.affiliation: Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
mesh_terms: D000328:Adult; D000395:Air Pollutants, Occupational / Q000633:toxicity*; D001554:Benzene / Q000633:toxicity; D005260:Female; D005742:Gasoline / Q000633:toxicity*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009153:Mutagens / Q000633:toxicity*; D016273:Occupational Exposure / Q000009:adverse effects*; D010636:Phenols / Q000633:toxicity / Q000652:urine; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors
chemical_list: D000395:Air Pollutants, Occupational; D005742:Gasoline; D007527:Isoenzymes; D009153:Mutagens; D010636:Phenols; D005982:Glutathione Transferase; D001554:Benzene
keywords: CBMN; GSTM1; GSTP1; GSTT1; gasoline pump workers; genetic polymorphism