title: Enhanced apatite-forming ability and antibacterial activity of porous anodic alumina embedded with CaO-SiO2-Ag2O bioactive materials.
abstract: In this study, to provide porous anodic alumina (PAA) with bioactivity and anti-bacterial properties, sol-gel derived bioactive CaO-SiO2-Ag2O materials were loaded onto and into PAA nano-pores (termed CaO-SiO2-Ag2O/PAA) by a sol-dipping method and subsequent calcination of the gel-glasses. The in vitro apatite-forming ability of the CaO-SiO2-Ag2O/PAA specimens was evaluated by soaking them in simulated body fluid (SBF). The surface microstructure and chemical property before and after soaking in SBF were characterized. Release of ions into the SBF was also measured. In addition, the antibacterial properties of the samples were tested against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The results showed that CaO-SiO2-Ag2O bioactive materials were successfully decorated onto and into PAA nano-pores. In vitro SBF experiments revealed that the CaO-SiO2-Ag2O/PAA specimens dramatically enhanced the apatite-forming ability of PAA in SBF and Ca, Si and Ag ions were released from the samples in a sustained and slow manner. Importantly, E. coli and S. aureus were both killed on the CaO-SiO2-Ag2O/PAA (by 100%) samples compared to PAA controls after 3 days of culture. In summary, this study demonstrated that the CaO-SiO2-Ag2O/PAA samples possess good apatite-forming ability and high antibacterial activity causing it to be a promising bioactive coating candidate for implant materials for orthopedic applications.
authors.affiliation: Key Lab of Eco-Textile, Ministry of Education, Donghua University, North Renmin Road 2999, Shanghai 201620, PR China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: synicn@dhu.edu.cn.
authors.forename: Siyu
authors.initials: S
authors.lastname: Ni
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Pengan
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Pathophysiology, Wenzhou Medical University, Wenzhou, 325027, PR China.
authors.forename: Shirong
authors.initials: S
authors.lastname: Ni
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hong
authors.affiliation: Department of Chemical Engineering, College of Engineering, Northeastern University, Boston, MA, 02115, USA; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001031:Apatites / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D017610:Calcium Compounds / Q000737:chemistry*; D004566:Electrodes; D004926:Escherichia coli / Q000187:drug effects / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010087:Oxides / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D018030:Silver Compounds / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D001031:Apatites; D001672:Biocompatible Materials; D017610:Calcium Compounds; D007477:Ions; D010087:Oxides; D018030:Silver Compounds; D012822:Silicon Dioxide; C040225:disilver oxide; C016538:lime; D000537:Aluminum Oxide
keywords: Antibacterial; Bioactive; Bone-like apatite; CaO–SiO(2); Porous anodic alumina; Silver