title: Endothelial Cell Viability of Donor Corneas Preserved in Eusol-C Corneal Storage Medium.
abstract: OBJECTIVE
  To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium.
  
  
  METHODS
  Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process.
  
  
  RESULTS
  Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm² at the beginning of the preservation (range, 1361-2899 cells/mm²). Donor endothelial cell density was between 1500 to 2000 cells/mm² in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm² on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day.
  
  
  CONCLUSIONS
  Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.
authors.affiliation: From the Izmir Bozyaka Training and Research Hospital, Department of Ophthalmology, Saim Çıkrıkçı Cd. No:59 Bozyaka, Izmir, Turkey.
authors.forename: Bora
authors.initials: B
authors.lastname: Yüksel
authors.forename: Umut Duygu
authors.initials: UD
authors.lastname: Uzunel
authors.forename: Tuncay
authors.initials: T
authors.lastname: Küsbeci
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002470:Cell Survival / Q000187:drug effects; D003080:Cold Temperature; D016039:Corneal Transplantation / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology / Q000637:transplantation; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000473:pathology / Q000637:transplantation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D013997:Time Factors; D020858:Tissue and Organ Harvesting / Q000379:methods*; D055815:Young Adult
chemical_list: C000621852:Eusol-C; D019188:Organ Preservation Solutions