title: Phosphorylated Peptides from Antarctic Krill (Euphausia superba) Prevent Estrogen Deficiency Induced Osteoporosis by Inhibiting Bone Resorption in Ovariectomized Rats.
abstract: In the current study, we investigated the improvement of phosphorylated peptides from Antarctic krill Euphausia superba (PP-AKP) on osteoporosis in ovariectomized rats. PP-AKP was supplemented to ovariectomized Sprague-Dawley rats for 90 days. The results showed that PP-AKP treatment remarkably prevented the reduction of bone mass and improved cancellous bone structure and biochemical properties. PP-AKP also significantly decreased serum contents of tartrate-resistant acid phosphatase (TRACP), cathepsin K (Cath-k), matrix metalloproteinases-9 (MMP-9), deoxypyridinoline (DPD), C-terminal telopeptide of collagen I (CTX-1), Ca, and P. Mechanism investigation revealed that PP-AKP significantly increased the osteoprotegerin (OPG)/receptor activator of nuclear factor κB ligand (RANKL) ratio in mRNA expression, protein expression, and serum content. Further research suggested that NF-κB signaling pathways were inhibited by suppressing the mRNA and protein expressions of nuclear factor of activated T-cells (NFATc1) and tumor necrosis factor receptor-associated factor 6 (TRAF6), diminishing the mRNA expression and phosphorylation of nuclear factor κB p65 (NF-κB p65), three key transcription factors in NF-κB pathways. These results suggest that PP-AKP can improve osteoporosis by inhibiting bone resorption via suppressing the activation of osteoclastogenesis related NF-κB pathways.
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Xia
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Tian
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China , Qingdao, Shandong Province 266003, China.
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000864:Antarctic Regions; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004967:Estrogens / Q000172:deficiency*; D033201:Euphausiacea / Q000737:chemistry*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D010052:Ovariectomy; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D050071:Bone Density Conservation Agents; D004967:Estrogens; D007527:Isoenzymes; D010455:Peptides; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D020780:Matrix Metalloproteinase 9
keywords: antarctic krill; bone mass; bone resorption; phosphorylated peptides; receptor activator of nuclear factor κB