title: Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma.
abstract: Primary squamous cell carcinoma of the thyroid is a rare and aggressive type of neoplasm, which is routinely treated with surgery; however, despite this, survival time is not commonly more than six months. Thus, the aim of the present study was to determine the efficacy of pseudolaric acid (PAB) as a therapeutic agent. PAB is an antitubulin agent, and in the present study, inhibition of the SW579 thyroid squamous cell carcinoma cell line by PAB was investigated. PAB was found to inhibit SW579 cell growth in a time‑ and dose‑dependent manner via interference in α‑tubulin polymerization. However, the inhibitory role of PAB in SW579 cells was not predominantly due to apoptosis, but was due to the cytostatic status resulting from cell cycle arrest. The present study proposes that this is the underlying mechanism of the antitumor properties of PAB. During cytostatis, autophagy was activated to sustain cell survival and SW579 cell migration was inhibited. Nuclear p53 expression was observed to be reduced, however the role of reduced p53 requires further investigation. Therefore, PAB induced cytostasis, which inhibited SW579 cell growth and therefore may function as an antitubulin therapeutic agent.
authors.affiliation: Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Peiyou
authors.initials: P
authors.lastname: Ren
authors.affiliation: Medicinal Chemistry, College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin 130000, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Cancer Center, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, College of Pharmacy, Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Bianbian
authors.initials: B
authors.lastname: Xue
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Dai
authors.affiliation: Acupuncture Department, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin 130000, P.R. China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Yu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D059565:G2 Phase Cell Cycle Checkpoints; D006801:Humans; D013964:Thyroid Neoplasms / Q000188:drug therapy*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D004224:Diterpenes; D014404:Tubulin; C058391:pseudolaric acid B