title: A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction.
abstract: The main function of annexin A1 (ANXA1), a member of the annexin superfamily, is to bind to cellular membranes in a Ca(2+)-dependent manner. In skeletal muscle, ANXA1 is thought to be involved in the repair of damaged membrane tissue and in the migration of muscle cells. We hypothesized that ANXA1 is one of the myokines secreted during muscle contractions to accelerate the repair of cell damage after contraction. Here we performed cell contractions by electric pulse stimulation; the results revealed that a fragmented form of ANXA1 was cleaved by calpain and selectively secreted from skeletal muscle cells by contraction. We therefore realized that muscle-contraction-induced calpain-dependent ANXA1 fragmentation has a wound-healing effect on damaged cells. This suggested that not the intact form but rather fragmented ANXA1 is a contraction-induced myokine.
authors.affiliation: Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa, 252-0880, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tamura
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Fumika
authors.initials: F
authors.lastname: Motai
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ito
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Kaede
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Manabe
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 1-1, Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan. fujiin@tmu.ac.jp.
authors.forename: Nobuharu L
authors.initials: NL
authors.lastname: Fujii
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D004560:Electricity; D013058:Mass Spectrometry; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D017305:Annexin A1
keywords: Annexin A1; C2C12 myotubes; Contraction; Secretion