title: Antioxidant Role for Lipid Droplets in a Stem Cell Niche of Drosophila.
abstract: Stem cells reside in specialized microenvironments known as niches. During Drosophila development, glial cells provide a niche that sustains the proliferation of neural stem cells (neuroblasts) during starvation. We now find that the glial cell niche also preserves neuroblast proliferation under conditions of hypoxia and oxidative stress. Lipid droplets that form in niche glia during oxidative stress limit the levels of reactive oxygen species (ROS) and inhibit the oxidation of polyunsaturated fatty acids (PUFAs). These droplets protect glia and also neuroblasts from peroxidation chain reactions that can damage many types of macromolecules. The underlying antioxidant mechanism involves diverting PUFAs, including diet-derived linoleic acid, away from membranes to the core of lipid droplets, where they are less vulnerable to peroxidation. This study reveals an antioxidant role for lipid droplets that could be relevant in many different biological contexts.
authors.affiliation: The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Bailey
authors.affiliation: Academic Unit of Clinical & Experimental Sciences, Faculty of Medicine, Sir Henry Wellcome Laboratories, Southampton General Hospital, Southampton SO16 6YD, UK.
authors.forename: Grielof
authors.initials: G
authors.lastname: Koster
authors.affiliation: National Resource for Imaging Mass Spectroscopy, Harvard Medical School and Brigham and Women's Hospital, Cambridge, MA 02139, USA.
authors.forename: Christelle
authors.initials: C
authors.lastname: Guillermier
authors.affiliation: The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: Elizabeth M A
authors.initials: EM
authors.lastname: Hirst
authors.affiliation: The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK.
authors.forename: James I
authors.initials: JI
authors.lastname: MacRae
authors.affiliation: National Resource for Imaging Mass Spectroscopy, Harvard Medical School and Brigham and Women's Hospital, Cambridge, MA 02139, USA.
authors.forename: Claude P
authors.initials: CP
authors.lastname: Lechene
authors.affiliation: Academic Unit of Clinical & Experimental Sciences, Faculty of Medicine, Sir Henry Wellcome Laboratories, Southampton General Hospital, Southampton SO16 6YD, UK.
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Postle
authors.affiliation: The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK. Electronic address: alex.gould@crick.ac.uk.
authors.forename: Alex P
authors.initials: AP
authors.lastname: Gould
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D049109:Cell Proliferation; D004330:Drosophila / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D055153:Stem Cell Niche / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D010100:Oxygen