title: Bacterial community structures of deep-sea water investigated by molecular biological techniques.
abstract: The aim of the present study was to investigate the bacterial community structures of deep-sea water (DSW) and surface seawater (SSW) samples in Japan by molecular biological techniques. DGGE analyses and pyrosequencing analysis revealed that bacterial community structures of DSW were diverse and differed from those of SSW. This is the first report on the horizontal variation of bacterial community structures of DSW throughout Japan. In addition, pyrosequencing analysis revealed that the number of phyla in DSW was larger than that in SSW, and specific phyla, such as Firmicutes and Planctomycetes, were characterized by a higher proportion of the bacterial community structure in DSW than in SSW. Taken together, these results indicate that a variety of bacteria that are specifically adapted to the DSW environments can be expected to be found in DSW, and DSW would thus be a potential resource for novel or unique microorganisms and compounds.
authors.affiliation: Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Terahara
authors.affiliation: Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan; DHC Corporation, 2-7-1, Minami-azabu, Minato-ku, Tokyo 106-8571, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, 5180, Kurokawa, Imizu-shi, Toyama 939-0398, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
authors.affiliation: Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: imada@kaiyodai.ac.jp.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Imada
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D007564:Japan; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Bacterial community structures; Deep-sea water (DSW); Denaturing gradient gel electrophoresis (DGGE); Pyrosequencing analysis