title: Genetic diversity, virulence potential and antimicrobial susceptibility of Listeria monocytogenes recovered from different sources in India.
abstract: Listeria monocytogenes isolates (n = 36) recovered from human and animal clinical cases and foods from different geographical regions of India were characterized using multiplex PCR-based serotyping, pulsed field gel electrophoresis (PFGE), in vitro and in vivo pathogenicity tests and antibiogram profiling. Multiplex PCR-based serotyping distributed L. monocytogenes isolates into 3 serogroups, of which 91.67% belonged to 4b, 4d, 4e serogroup, followed by 5.56% to 1/2a, 3a and 2.78% to 1/2b, 3b serogroups. PFGE analysis using ApaI and AscI restriction enzymes revealed 17 pulsotypes among 36 L. monocytogenes isolates with 6 major clusters having similar fingerprint profile within their cluster and 11 unique fingerprint profiles. Interestingly, PFGE analysis inferred that foods of animal origin could be a significant source of infection for spread of listeriosis among human populations. Furthermore, on comparison of in vitro and in vivo pathogenicity tests, an overall good correlation was observed between hemolytic titer assay and chick embryo inoculation test as most of the isolates with a hemolytic titer of ≥ 16 were found to be lethal to chick embryo. All the isolates were found to be susceptible to tested antimicrobials except for one animal isolate which showed resistance towards co-trimoxazole.
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Negi
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Jess
authors.initials: J
authors.lastname: Vergis
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Deepthi
authors.initials: D
authors.lastname: Vijay
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dhaka
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: S V S
authors.initials: SV
authors.lastname: Malik
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: ICAR Research Complex for Goa, Old Goa, 403 402, India.
authors.forename: Krupali V
authors.initials: KV
authors.lastname: Poharkar
authors.affiliation: ICAR Research Complex for Goa, Old Goa, 403 402, India.
authors.forename: Swapnil P
authors.initials: SP
authors.lastname: Doijad
authors.affiliation: ICAR Research Complex for Goa, Old Goa, 403 402, India.
authors.forename: Sukhadeo B
authors.initials: SB
authors.lastname: Barbuddhe
authors.affiliation: Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, 211 007, India.
authors.forename: Pramod W
authors.initials: PW
authors.lastname: Ramteke
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India deepak.rawool@yahoo.com.
authors.forename: Deepak B
authors.initials: DB
authors.lastname: Rawool
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002642:Chick Embryo; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004195:Disease Models, Animal; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D014644:Genetic Variation; D005838:Genotype; D006461:Hemolysis; D006801:Humans; D007194:India; D008089:Listeria monocytogenes / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D008088:Listeriosis / Q000382:microbiology* / Q000662:veterinary*; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D065288:Serogroup; D016019:Survival Analysis; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors
keywords: Listeria monocytogenes; PFGE; antimicrobials; genetic diversity; listeriosis; serotyping