title: [THE CHROMOGENIC SYNTHETIC MEDIUM "KLEBSIELLA 5-ASK CHROM-C" FOR ISOLATION AND IDENTIFICATION OF KLEBSIELLAE].
abstract: The chromogenic synthetic medium "Klebsiella 5-ASK CHROM-C was developedfor isolation and identification of klebsiellae of species of K. pneumoniae subsp. pneumoniae K. oxytoca, K. mobilis according chromogenic reaction to enzyme 5-aminosalycilate decarboxylase as a unique marker of genus Klebsiella. The L-proline and L-calcium glutamate are used as a source of nitrogen and carbon in medium. The consistency of composition of growth medium that ensure its regularity. The diagnostic sensitivity of chromogenic medium is 95.3 ± 1.7%; diagnostic specificity is 100%; analytical sensitivity is 1-2 colony-forming units per ml-1. The identification of Klebsiella is achieved simultaneously with their isolation during 24 = 48 hours. The test of 5-ASK decarboxylase using two chromogenic mediums "Klebsiella 5-ASK CHROM-C" permits identifying additionally K. pneumoniae subsp, ozaenae, K .pneumoniae subsp. Rhinoscleromatis.
authors.forename: E P
authors.initials: EP
authors.lastname: Sivolodskii
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D002863:Chromogenic Compounds / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry; D007709:Klebsiella / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D011392:Proline / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D002863:Chromogenic Compounds; D003470:Culture Media; D018698:Glutamic Acid; D011392:Proline; D002262:Carboxy-Lyases