title: Phylogenetic diversity of culturable endophytic fungi in Dongxiang wild rice (Oryza rufipogon Griff), detection of polyketide synthase gene and their antagonistic activity analysis.
abstract: The biodiversity of plant endophytic fungi is enormous, numerous competent endophytic fungi are capable of providing different forms of fitness benefits to host plants and also could produce a wide array of bioactive natural products, which make them a largely unexplored source of novel compounds with potential bioactivity. In this study, we provided a first insights into revealing the diversity of culturable endophytic fungi in Dongxiang wild rice (Oryza rufipogon Griff.) from China using rDNA-ITS phylogenetic analysis. Here, the potential of fungi in producing bioactive natural products was estimated based on the beta-ketosynthase detected in the polyketide synthase (PKS) gene cluster and on the bioassay of antagonistic activity against two rice phytopathogens Thanatephorus cucumeris and Xanthomonas oryzae. A total of 229 endophytic fungal strains were validated in 19 genera. Among the 24 representative strains, 13 strains displayedantagonistic activity against the phytopathogens. Furthermore, PKS genes were detected in 9 strains, indicating their potential for synthesising PKS compounds. Our study confirms the phylogenetic diversity of endophytic fungi in O. rufipogon G. and highlights that endophytic fungi are not only promising resources of biocontrol agents against phytopathogens of rice plants, but also of bioactive natural products and defensive secondary metabolites.
authors.affiliation: Key Lab of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Bo Liang
authors.initials: BL
authors.lastname: Gao
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China; Science and Technology College, Jiangxi University of Traditional Chinese Medicine, Nanchang 330025, China.
authors.forename: Xi Xi
authors.initials: XX
authors.lastname: Li
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Zhi Bin
authors.initials: ZB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Ri Ming
authors.initials: RM
authors.lastname: Yan
authors.affiliation: Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Hui Lin
authors.initials: HL
authors.lastname: Yang
authors.affiliation: Key Lab of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China; Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang 330022, China. Electronic address: zhudu12@163.com.
authors.forename: Du
authors.initials: D
authors.lastname: Zhu
mesh_terms: D002681:China; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D005658:Fungi / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D056265:Microbial Interactions; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D048630:Polyketide Synthases
keywords: Antagonistic activity; Fungal diversity; Isolation; Molecular identification; Phylogenetic analysis; Polyketides