title: Study of the boron levels in serum after implantation of different ratios nano-hexagonal boron nitride-hydroxy apatite in rat femurs.
abstract: Boron and its derivatives are effective in bone recovery and osteointegration. However, increasing the boron levels in body liquids may cause toxicity. The aim of our study is to investigate serum boron levels using ICP-MS after implantation of different ratios of nano-hBN-HA composites in rat femurs. All rats were (n=126) divided into five experimental groups (n=24) and one healthy group (6 rats); healthy (Group1), femoral defect + %100 HA (Group2), femoral defect + %2.5 hBN + %97.5 HA (Group3), femoral defect + %5 hBN + %95 HA (Group4), femoral defect + %10 hBN + %90 HA (Group5), femoral defect + %100 hBN (Group6). The femoral defect was created in the distal femur (3mm drill-bit). Each implant group was divided into four different groups (n=24) also 6 rats sacrificed for each groups in one week intervals during four weeks. In our results; at 1, 2, 3, and 4 weeks after implantation near bone tissue, serum levels of boron were evaluated using ICP-MS. We demonstrated that neither short-term nor long-term implantation of hBN-HA composite resulted in statistically increased serum boron levels in experimental groups compared to healthy group. In conclusion, this study investigated the implant material produced form hBN-HA for the first time. Our data suggest that hBN is a new promising target for biomaterial and implant bioengineers.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey. Electronic address: alptugatila@yahoo.com.
authors.forename: Alptug
authors.initials: A
authors.lastname: Atila
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum 25240, Turkey.
authors.forename: Zekai
authors.initials: Z
authors.lastname: Halici
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum 25240, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Cadirci
authors.affiliation: Department of Pharmacology and Toxicology, School of Veterinary Medicine, Ataturk University, Erzurum 25240, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Karakus
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey.
authors.forename: Saziye Sezin
authors.initials: SS
authors.lastname: Palabiyik
authors.affiliation: Department of Material Science and Engineering, Faculty of Engineering, Anadolu University, Eskisehir 26555, Turkey.
authors.forename: Nuran
authors.initials: N
authors.lastname: Ay
authors.affiliation: Sabancı University Nanotechnology Research and Application Center (SUNUM), Istanbul 34956, Turkey.
authors.forename: Feray
authors.initials: F
authors.lastname: Bakan
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul 34755, Turkey.
authors.forename: Sahin
authors.initials: S
authors.lastname: Yilmaz
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001895:Boron / Q000097:blood* / Q000378:metabolism; D001896:Boron Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D005269:Femur / Q000601:surgery*; D053761:Nanocomposites / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D001896:Boron Compounds; C017282:boron nitride; D001895:Boron
keywords: Boron; Femur; ICP-MS; Nano-hexagonal boron nitride; Rat; Serum