title: Novel qPCR systems for olive (Olea europaea L.) authentication in oils and food.
abstract: The traceability of olive oil is an unresolved issue that remains a challenge. In this field, DNA-based techniques are very powerful tools for discrimination that are less negatively influenced by environmental conditions than other techniques. More specifically, quantitative real time PCR (qPCR) achieves a high degree of sensitivity, although the DNA that it can directly isolate from these oils presents drawbacks. Our study reports the analysis of eight systems, in order to determine their suitability for olive detection in oil and oil-derived foodstuffs. The eight systems were analyzed on the basis of their sensitivity and specificity in the qPCR assay, their relative sensitivity to olive DNA detection and DNA mixtures, their sensitivity and specificity to olive in vegetable oils and the detection of olive in commercial products. The results show that the PetN-PsbM system, designed in this study, is a suitable and reliable technique in relation to olive oil and olive ingredients in both food authentication and food safety processes.
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Ramos-Gómez
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: María D
authors.initials: MD
authors.lastname: Busto
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain.
authors.forename: Silvia M
authors.initials: SM
authors.lastname: Albillos
authors.affiliation: Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos, s/n, 09001 Burgos, Spain. Electronic address: nortega@ubu.es.
authors.forename: Natividad
authors.initials: N
authors.lastname: Ortega
mesh_terms: D003314:Corn Oil / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005229:Fatty Acids, Monounsaturated / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D031658:Olea / Q000235:genetics*; D000069463:Olive Oil / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D000074262:Rapeseed Oil; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012715:Sesame Oil / Q000032:analysis; D013024:Soybean Oil / Q000032:analysis; D000074242:Sunflower Oil
chemical_list: D018744:DNA, Plant; D005229:Fatty Acids, Monounsaturated; D000069463:Olive Oil; D010938:Plant Oils; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D013024:Soybean Oil; D003314:Corn Oil; D012715:Sesame Oil
keywords: Authentication; Olive oil; qPCR