title: Flow Cytometry-Based Assessment of Antibody Function Against Malaria Pre-erythrocytic Infection.
abstract: The development of new interventional strategies against pre-erythrocytic malaria is hampered by the lack of standardized approaches to assess inhibition of sporozoite infection of hepatocytes. The following methodology, based on flow cytometry, can be used to quantitatively assess P. falciparum sporozoite infection in vitro in medium throughput. In addition to assessing the efficacy of antibodies, this assay has a wide variety of applications for investigating basic science questions about the malaria liver stage. This approach is easily applied in a variety of laboratory settings, assesses the functionality of antibody responses against malaria sporozoites, and can be adapted for the limited quantities of sample which are typically available from clinical investigations.
authors.affiliation: Center for Infectious Disease Research, 307 Westlake Ave. N, Suite 500, Seattle, WA, 98109, USA.
authors.forename: Alyse N
authors.initials: AN
authors.lastname: Douglass
authors.affiliation: Center for Infectious Disease Research, 307 Westlake Ave. N, Suite 500, Seattle, WA, 98109, USA.
authors.forename: Peter G
authors.initials: PG
authors.lastname: Metzger
authors.affiliation: Center for Infectious Disease Research, 307 Westlake Ave. N, Suite 500, Seattle, WA, 98109, USA.
authors.forename: Stefan H I
authors.initials: SH
authors.lastname: Kappe
authors.affiliation: Center for Infectious Disease Research, 307 Westlake Ave. N, Suite 500, Seattle, WA, 98109, USA. alexis.kaushansky@cidresearch.org.
authors.forename: Alexis
authors.initials: A
authors.lastname: Kaushansky
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D017780:Malaria Vaccines / Q000276:immunology* / Q000627:therapeutic use; D016778:Malaria, Falciparum / Q000276:immunology* / Q000517:prevention & control; D008967:Molecular Biology / Q000379:methods; D010963:Plasmodium falciparum / Q000276:immunology / Q000472:pathogenicity; D034101:Sporozoites / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D017780:Malaria Vaccines
keywords: Flow cytometry; Liver; Malaria; Sporozoite