title: Short-term hyperprolactinemia decreases allergic inflammatory response of the lungs.
abstract: OBJECTIVE
  Prolactin is a major immunomodulator. The present study evaluated the effects of short-term hyperprolactinemia induced by domperidone before ovalbumin antigenic challenge on the lung's allergic inflammatory response.
  
  
  METHODS
  To induce hyperprolactinemia, domperidone was injected in rats at a dose of 5.1mg·kg(-1) per day, i.p., for 5days from 10th to 14th day after OVA immunization. Total and differential leukocyte counts from bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood were analyzed. The percentages of mucus and collagen production were evaluated. Levels of corticosterone and prolactin in serum, interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor α (TNF-α) in lung explants supernatants were measured and interferon gamma (IFN-γ) in bronchiolar lavage cells suspensions (BAL) was measured.
  
  
  RESULTS
  The rats that were subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bronchoalveolar lavage, cellularity decrease in femoral marrow lavage fluid, a lower percentage of mucus, and an increase in lung IL-4, IL-6, IL-10, TNF-α and IFN-γ expression.
  
  
  CONCLUSIONS
  Hyperprolactinemia induced before antigenic challenge decreased allergic lung inflammation. These data suggest that prolactin may play a role in the pathophysiology of asthma. The present study demonstrates a prospective beneficial side effect of domperidone for asthmatic patients.
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil; Facultad de Ciencias Agropecuarias y Recursos Naturales, Programa de Medicina Veterinaria y Zootecnia, Universidad de los Llanos, Villavicencio, Colombia.
authors.forename: Julieta E
authors.initials: JE
authors.lastname: Ochoa-Amaya
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Eduardo K
authors.initials: EK
authors.lastname: Hamasato
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Carla N
authors.initials: CN
authors.lastname: Tobaruela
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Nicolle
authors.initials: N
authors.lastname: Queiroz-Hazarbassanov
authors.affiliation: Laboratório de Neuroendocrinologia da Reprodução, Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, São Paulo, SP, Brazil.
authors.forename: Janete A
authors.initials: JA
authors.lastname: Anselmo Franci
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: João
authors.initials: J
authors.lastname: Palermo-Neto
authors.affiliation: Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde, Universidade Nove de Julho, São Paulo, Brazil.
authors.forename: Flavia R
authors.initials: FR
authors.lastname: Greiffo
authors.affiliation: Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde, Universidade Nove de Julho, São Paulo, Brazil.
authors.forename: Auriléia Aparecida
authors.initials: AA
authors.lastname: de Britto
authors.affiliation: Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde, Universidade Nove de Julho, São Paulo, Brazil.
authors.forename: Rodolfo Paula
authors.initials: RP
authors.lastname: Vieira
authors.affiliation: Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde, Universidade Nove de Julho, São Paulo, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Ligeiro de Oliveira
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Cristina de O
authors.initials: Cde O
authors.lastname: Massoco
authors.affiliation: Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: lfelicio@usp.br.
authors.forename: Luciano F
authors.initials: LF
authors.lastname: Felicio
mesh_terms: D000818:Animals; D001249:Asthma / Q000097:blood / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000097:blood / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006966:Hyperprolactinemia / Q000097:blood / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011388:Prolactin / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D016207:Cytokines; D011388:Prolactin
keywords: Asthma; Domperidone; Lung; Prolactin