title: Different gene expression profiles between normal and thermally selected strains of rainbow trout, Oncorhynchus mykiss, as revealed by comprehensive transcriptome analysis.
abstract: A high-temperature selected (HT) strain of rainbow trout was established from the Donaldson (DS) strain by traditional selective breeding in Japan. The aim of this study is to investigate genes related to upper temperature tolerance in this strain utilizing next generation sequencer (NGS), and to establish comprehensive and comparable datasets in brain, liver, muscle, heart and gill tissues between the HT and DS strains. After assembling, clustering and filtering, 242,530 contigs were obtained. Among them, 7624 transcripts had at least 10 counts in expression analysis in all tissues and used as references. BLASTX homology search showed that 7329 transcripts matched with known genes. Compared to the DS strain, the HT strain expressed 90, 775, 349, 188 and 194 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in the case of fish before heat-exposure treatment. Meanwhile, the HT strain expressed 292, 363, 433, 322 and 211 genes 2 folds or more in brain, liver, muscle, heart, and gill, respectively in case of fish after heat-exposure treatment. Many of heat shock protein family genes and transcription factor AP-1 related genes were highly expressed in all tissues of the HT strain compared with the DS strain. These results suggest that these genes play key roles in upper temperature tolerance. These comprehensive and comparable datasets will offer broad visions for upper temperature tolerance in fish species.
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Engkong
authors.initials: E
authors.lastname: Tan
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
authors.forename: Shigeharu
authors.initials: S
authors.lastname: Kinoshita
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwano-ha, Kashiwa, Chiba 277-8561, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Kobayashi Branch, Miyazaki Prefectural Fisheries Research Institute, Kobayashi, Miyazaki 886-0005, Japan.
authors.forename: Toshinao
authors.initials: T
authors.lastname: Ineno
authors.affiliation: Kobayashi Branch, Miyazaki Prefectural Fisheries Research Institute, Kobayashi, Miyazaki 886-0005, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tamaki
authors.affiliation: Kobayashi Branch, Miyazaki Prefectural Fisheries Research Institute, Kobayashi, Miyazaki 886-0005, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Kera
authors.affiliation: Nikko Station, National Research Institute of Aquaculture, Fisheries Research Agency, Tochigi 321-1661, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Muto
authors.affiliation: Nikko Station, National Research Institute of Aquaculture, Fisheries Research Agency, Tochigi 321-1661, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yada
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Hatsukaichi, Hiroshima 739-0452, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Kitamura
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan. Electronic address: asakawa@mail.ecc.u-tokyo.ac.jp.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Asakawa
authors.affiliation: School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan. Electronic address: swatabe@kitasato-u.ac.jp.
authors.forename: Shugo
authors.initials: S
authors.lastname: Watabe
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D017686:Oncorhynchus mykiss / Q000235:genetics*; D059467:Transcriptome
keywords: Heat shock protein; High-temperature selected strain; Next generation sequencing; RNAseq; Transcription factor AP-1