title: Genetic diversity for mycorrhizal symbiosis and phosphate transporters in rice.
abstract: Phosphorus (P) is a major plant nutrient and developing crops with higher P-use efficiency is an important breeding goal. In this context we have conducted a comparative study of irrigated and rainfed rice varieties to assess genotypic differences in colonization with arbuscular mycorrhizal (AM) fungi and expression of different P transporter genes. Plants were grown in three different soil samples from a rice farm in the Philippines. The data show that AM symbiosis in all varieties was established after 4 weeks of growth under aerobic conditions and that, in soil derived from a rice paddy, natural AM populations recovered within 6 weeks. The analysis of AM marker genes (AM1, AM3, AM14) and P transporter genes for the direct Pi uptake (PT2, PT6) and AM-mediated pathway (PT11, PT13) were largely in agreement with the observed root AM colonization providing a useful tool for diversity studies. Interestingly, delayed AM colonization was observed in the aus-type rice varieties which might be due to their different root structure and might confer an advantage for weed competition in the field. The data further showed that P-starvation induced root growth and expression of the high-affinity P transporter PT6 was highest in the irrigated variety IR66 which also maintained grain yield under P-deficient field conditions.
authors.affiliation: Southern Cross Plant Science, Southern Cross University, PO Box 57 Lismore NSW 2480, Australia.
authors.forename: Kwanho
authors.initials: K
authors.lastname: Jeong
authors.affiliation: International Rice Research Institute (IRRI), Crop and Environmental Sciences Division, 7777 Metro Manila, Philippines.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mattes
authors.affiliation: International Rice Research Institute (IRRI), Crop and Environmental Sciences Division, 7777 Metro Manila, Philippines.
authors.forename: Sheryl
authors.initials: S
authors.lastname: Catausan
authors.affiliation: Seoul National University, College of Agriculture and Life Sciences, Plant Genomics and Breeding Institute, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors.forename: Joong Hyoun
authors.initials: JH
authors.lastname: Chin
authors.affiliation: University of Cambridge, Department of Plant Sciences, Downing Street, Cambridge, CB2, 3EA, UK.
authors.forename: Uta
authors.initials: U
authors.lastname: Paszkowski
authors.affiliation: Australian Center For Plant Functional Genomics (ACPFG), Adelaide, SA 5064, Australia.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Heuer
mesh_terms: D000383:Agriculture; D018556:Crops, Agricultural / Q000235:genetics / Q000382:microbiology; D014644:Genetic Variation; D005838:Genotype; D038821:Mycorrhizae; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D028061:Phosphate Transport Proteins / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism; D012987:Soil; D013045:Species Specificity; D013559:Symbiosis
chemical_list: D028061:Phosphate Transport Proteins; D010710:Phosphates; D012987:Soil
keywords: Mycorrhiza; P deficiency tolerance; phosphate transporters; rice; roots