title: Characterization of a new aryl-alcohol oxidase secreted by the phytopathogenic fungus Ustilago maydis.
abstract: The discovery of novel fungal lignocellulolytic enzymes is essential to improve the breakdown of plant biomass for the production of second-generation biofuels or biobased materials in green biorefineries. We previously reported that Ustilago maydis grown on maize secreted a diverse set of lignocellulose-acting enzymes including hemicellulases and putative oxidoreductases. One of the most abundant proteins of the secretome was a putative glucose-methanol-choline (GMC) oxidoreductase. The phylogenetic prediction of its function was hampered by the few characterized members within its clade. Therefore, we cloned the gene and produced the recombinant protein to high yield in Pichia pastoris. Functional screening using a library of substrates revealed that this enzyme was able to oxidize several aromatic alcohols. Of the tested aryl-alcohols, the highest oxidation rate was obtained with 4-anisyl alcohol. Oxygen, 1,4-benzoquinone, and 2,6-dichloroindophenol can serve as electron acceptors. This GMC oxidoreductase displays the characteristics of an aryl-alcohol oxidase (E.C.1.1.3.7), which is suggested to act on the lignin fraction in biomass.
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Couturier
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Mathieu
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
authors.forename: Ai
authors.initials: A
authors.lastname: Li
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Navarro
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|CNRS, UMR7257 Architecture et Fonction des Macromolécules Biologiques, F-13288, Marseille, France.|INRA, USC1408 Architecture et Fonction des Macromolécules Biologiques, F-13288, Marseille, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Drula
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Haon
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France.
authors.forename: Sacha
authors.initials: S
authors.lastname: Grisel
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, 1190, Vienna, Austria.
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.affiliation: INRA, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France. jean-guy.berrin@univ-amu.fr.|Marseille Université, UMR1163 BBF, Polytech'Marseille, F-13288, Marseille, France. jean-guy.berrin@univ-amu.fr.|INRA, Laboratoire de Biodiversité et Biotechnologie Fongique, Marseille, France. jean-guy.berrin@univ-amu.fr.
authors.forename: Jean-Guy
authors.initials: JG
authors.lastname: Berrin
mesh_terms: D015086:2,6-Dichloroindophenol / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D016227:Benzoquinones / Q000378:metabolism; D018533:Biomass; D004579:Electron Transport; D008031:Lignin / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010802:Phylogeny; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014588:Ustilago / Q000201:enzymology* / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D011994:Recombinant Proteins; C036909:lignocellulose; D008031:Lignin; D015086:2,6-Dichloroindophenol; D000429:Alcohol Oxidoreductases; C077759:aryl-alcohol oxidase; D010100:Oxygen
keywords: AA3; Aryl-alcohol oxidase; Biorefinery; CAZy; Lignin; Ustilago maydis