title: Sensitive determination of kaempferol using carbon dots as a fluorescence probe.
abstract: In this study, a new sensitive and convenient method for the determination of kaempferol (Kae) based on the fluorescence quenching of fluorescent carbon dots (C-dots) was developed. The C-dots were prepared by simply mixing acetic acid, water and diphosphorus pentoxide. This green synthesis approach proceeds rapidly and gives large quantities of C-dots. The fluorescence of the C-dots decreased obviously with the increase of Kae concentration. The effect of other interfering substances on the fluorescence intensity of C-dots showed low interference. Under the optimum conditions, a linear correlation was established between fluorescence intensity ratio Fo/F and the concentration of Kae in the range of 3.5-49 µM with a detection limit (S/N=3) of 38.4 nM. The proposed method has been successfully applied to determination of Kae in xindakang tablets and human serum samples with recovery in the range of 94.6-109.0%. The C-dots could be a promising fluorescence probe for the detection of Kae owing to its low-cost production, easy operation, low cytotoxicity, and excellent biocompatibility.
authors.affiliation: School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, PR China; College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, PR China; College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China. Electronic address: feng-feng64@263.net.
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, and Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, PR China.
authors.forename: Man Chin
authors.initials: MC
authors.lastname: Paau
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, and Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, and Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China.
authors.forename: Zezhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Bai
authors.affiliation: College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, PR China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, and Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, PR China. Electronic address: mmfchoi@gmail.com.
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D044949:Kaempferols / Q000032:analysis* / Q000097:blood / Q000737:chemistry*; D007700:Kinetics; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D044949:Kaempferols; C006552:kaempferol
keywords: Carbon dots; Fluorescence probe and kaempferol