title: Maslinic Acid Protected PC12 Cells Differentiated by Nerve Growth Factor against β-Amyloid-Induced Apoptosis.
abstract: β-Amyloid peptide (Abeta) was used to induce apoptosis in PC12 cells differentiated by nerve growth factor, and the protective activities of maslinic acid (MA) at 2-16 μM were examined. Abeta treatment lowered Bcl-2 expression, raised Bax expression, and decreased cell viability. MA pretreatments decreased Bax expression, raised the Bcl-2/Bax ratio, and increased cell viability. MA pretreatments retained glutathione content and decreased subsequent Abeta-induced release of reactive oxygen species, tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. Abeta treatment up-regulated protein expression of p47(phox), gp91(phox), mitogen-activated protein kinase, advanced glycation end product receptor (RAGE), and nuclear factor-κ B (NF-κB). MA pretreatments at 2-16 μM suppressed the expression of proteins including gp91(phox), p47(phox), p-p38, and NF-κB p65, at 4-16 μM down-regulated RAGE and NF-κB p50 expression, and at 8 and 16 μM reduced p-ERK1/2 expression. These novel findings suggest that maslinic acid is a potent compound against Abeta-induced cytotoxicity.
authors.affiliation: School of Medicine, China Medical University , Taichung City, Taiwan.|Department of Neurology, China Medical University Hospital , Taichung City, Taiwan.
authors.forename: Yu-wan
authors.initials: YW
authors.lastname: Yang
authors.affiliation: Department of Nutrition, China Medical University , Taichung City, Taiwan.
authors.forename: Chia-wen
authors.initials: CW
authors.lastname: Tsai
authors.affiliation: Department of Health and Nutrition Biotechnology, Asia University , Taichung City, Taiwan.
authors.forename: Mei-chin
authors.initials: MC
authors.lastname: Mong
authors.affiliation: Department of Nutrition, China Medical University , Taichung City, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University , Taichung City, Taiwan.
authors.forename: Mei-chin
authors.initials: MC
authors.lastname: Yin
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002477:Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D016229:Amyloid beta-Peptides; D015850:Interleukin-6; D016328:NF-kappa B; D020932:Nerve Growth Factor; D017382:Reactive Oxygen Species; D014315:Triterpenes; D048051:p38 Mitogen-Activated Protein Kinases; C412811:maslinic acid; D009828:Oleanolic Acid
keywords: Abeta; NADPH oxidase; RAGE; maslinic acid