title: Cloning of the short-tailed Gyeongju Donggyeong dog via SCNT: conserving phenotypic inheritance.
abstract: Somatic cell nuclear transfer is a useful tool to maintain genetic information of animals. The Gyeongju Donggyeong dog is a breed registered as natural monument in Korea. The unique feature of the Donggyeong dog is its tail, as the Donggyeong dog can be classified as either short tailed or tailless. The aim of this study was to preserve the Donggyeong dog's unique feature by cloning. Fibroblasts were obtained from a short-tailed Donggyeong dog. In vivo matured oocytes were enucleated, microinjected with a donor cell and fused electrically. Reconstructed embryos were transferred to six recipient dogs. One surrogate became pregnant, and one short-tailed Donggyeong dog was delivered. This study demonstrated that the phenotype of the Donggyeong dog could be conserved by somatic cell nuclear transfer.
authors.affiliation: Department of Theriogenology and Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea, 151-742.
authors.forename: Yoo Bin
authors.initials: YB
authors.lastname: Choi
authors.forename: Geon A
authors.initials: GA
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Oh
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Jo
authors.forename: Erif Maha Nugraha
authors.initials: EM
authors.lastname: Setyawan
authors.forename: Seok Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Byeong Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D019976:Cloning, Organism / Q000662:veterinary*; D004285:Dogs; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005347:Fibroblasts; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary