title: Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.
abstract: A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.
authors.affiliation: Okazaki Institute for Integrative Bioscience, Institute for Molecular Science, National Institutes of Natural Sciences, Aichi, 444-8787, Japan. iino@ims.ac.jp.|Department of Functional Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies (SOKENDAI), Kanagawa, 240-0193, Japan. iino@ims.ac.jp.
authors.forename: Ryota
authors.initials: R
authors.lastname: Iino
authors.affiliation: Technical Division, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Shouichi
authors.initials: S
authors.lastname: Sakakihara
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Matsumoto
authors.affiliation: Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Nishino
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004361:Drug Tolerance / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D055616:Microtechnology / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D059010:Single-Cell Analysis / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Drug tolerance; Microdevice; Microdroplet array; Microfabrication; Micropipette; Optical microscopy; Persister; Pseudomonas aeruginosa; Single-cell analysis