title: Gomisin J Inhibits Oleic Acid-Induced Hepatic Lipogenesis by Activation of the AMPK-Dependent Pathway and Inhibition of the Hepatokine Fetuin-A in HepG2 Cells.
abstract: The aim of our study is to investigate the molecular mechanism of gomisin J from Schisandra chinensis on the oleic acid (OA)-induced lipid accumulation in HepG2 cells. Gomisin J attenuated lipid accumulation in OA-induced HepG2 cells. It also suppressed the expression of lipogenic enzymes and inflammatory mediators and increased the expression of lipolytic enzymes in OA-induced HepG2 cells. Furthermore, the use of specific inhibitors and fetuin-A siRNA and liver kinase B1 (LKB1) siRNA transfected cells demonstrated that gomisin J regulated lipogenesis and lipolysis via inhibition of fetuin-A and activation of an AMP-activated protein kinase (AMPK)-dependent pathway in HepG2 cells. Our results showed that gomisin J suppressed lipid accumulation by regulating the expression of lipogenic and lipolytic enzymes and inflammatory molecules through activation of AMPK, LKB1, and Ca(2+)/calmodulin-dependent protein kinase II and inhibition of fetuin-A in HepG2 cells. This suggested that gomisin J has potential benefits in treating nonalcoholic fatty liver disease.
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon 210-340, Korea.
authors.forename: Myungsuk
authors.initials: M
authors.lastname: Kim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon 210-340, Korea.|Department of Chemistry, Gangneung-Wonju National University , Gangneung, Gangwon-do 210-702, Korea.
authors.forename: Sue Ji
authors.initials: SJ
authors.lastname: Lim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon 210-340, Korea.
authors.forename: Hee-Ju
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon 210-340, Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute , Gangwon 210-340, Korea.
authors.forename: Chu Won
authors.initials: CW
authors.lastname: Nho
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D060749:alpha-2-HS-Glycoprotein / Q000235:genetics / Q000378:metabolism*
chemical_list: D017705:Lignans; D010936:Plant Extracts; D011083:Polycyclic Compounds; D060749:alpha-2-HS-Glycoprotein; D019301:Oleic Acid; D055372:AMP-Activated Protein Kinases; C082091:gomisin J
keywords: AMPK; CaMKII; LKB1; fetuin-A; gomisin J