title: Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for SiRNA Delivery.
abstract: Engineered PEG-cleavable catiomers based on poly-L-lysine have been developed as nonviral gene vectors, which have been found to be one of important methods to balance "PEG dilemma." In this protocol, we aim at the standardization of the method and procedure of PEG-cleavable catiomers. Major steps including ring-opening polymerization (ROP) of ε-benzyloxycarbonyl-L-lysine N-carboxyanhydride (zLL-NCA) monomers to yield PEG-cleavable polylysine, examination on bio-stability and bio-efficacy of its gene complexes are described.
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Dong
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Cai
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China.
authors.forename: Tianbin
authors.initials: T
authors.lastname: Ren
authors.affiliation: Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science (iNANO), Tongji University School of Medicine, Shanghai, China. yongyong_li@tongji.edu.cn.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Li
mesh_terms: D001692:Biological Transport; D002460:Cell Line; D004220:Disulfides / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D004355:Drug Stability; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D058105:Polymerization; D034741:RNA, Small Interfering / Q000737:chemistry*
chemical_list: D004220:Disulfides; D004337:Drug Carriers; D034741:RNA, Small Interfering; D011107:Polylysine; D011092:Polyethylene Glycols
keywords: Cleavable PEGylation; Disulfide -bridged; Gene delivery; Nonviral gene vector; Poly-L-lysine (PLL)