title: The in vitro effect of Lambda-cyhalothrin on quality and antioxidant responses of rainbow trout Oncorhynchus mykiss spermatozoa.
abstract: There is little information in the scientific literature about effect of in vitro exposure of fish spermatozoa to pesticides. In vitro effect of Lambda-cyhalothrin (LCT) on sperm quality and oxidative stress has not been fully explored yet. The effects of LCT, which is a synthetic pyrethroid insecticide, on quality and oxidative stress of spermatozoa were investigated in vitro due to extensively use to control a wide range of insect pests in agriculture, public health, and homes and gardens. To explore the potential in vitro toxicity of LCT, fish spermatozoa were incubated with different concentrations of LCT (0.6, 1.2 and 2.4 μg/L) for 2h. Reduced glutathione (GSH), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in spermatozoa were analyzed for determination of oxidant and antioxidant balance. Our results indicated that the percentage and duration of sperm motility significantly decreased with exposure to LCT. Activity of GSH-Px and MDA (P<0.05) and GSH levels (P<0.05) increased in a concentration-dependent manner while CAT activity decreased (P<0.05). In conclusion, the oxidant and antioxidant status and sperm quality were affected by increasing concentrations of LCT.
authors.affiliation: Tunceli University, Fisheries Faculty, 62000 Tunceli, Turkey. Electronic address: filizkutluyer@hotmail.com.
authors.forename: Filiz
authors.initials: F
authors.lastname: Kutluyer
authors.affiliation: Tunceli University, Faculty of Engineering, Department of Food Engineering, 62000 Tunceli, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Erişir
authors.affiliation: Fırat University, Faculty of Veterinary, Department of Basic Sciences, Elazığ, Turkey.
authors.forename: Fulya
authors.initials: F
authors.lastname: Benzer
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Department of Biology, 48000 Muğla, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Öğretmen
authors.affiliation: Muğla Sıtkı Koçman University, Faculty of Science, Department of Biology, 48000 Muğla, Turkey.
authors.forename: Burak Evren
authors.initials: BE
authors.lastname: İnanan
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009570:Nitriles / Q000633:toxicity*; D017686:Oncorhynchus mykiss / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects*; D011722:Pyrethrins / Q000633:toxicity*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione; C037304:cyhalothrin
keywords: Lambda-cyhalothrin; Oncorhynchus mykiss; Oxidative stress indices; Rainbow trout; Spermatozoa