title: Optimisation of pressurised water extraction of polysaccharides from blackcurrant and its antioxidant activity.
abstract: Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was investigated using a response surface methodology (RSM). The optimal conditions for PWE were: time 51min, pressure 1.6MPa, and temperature 52°C. Under these conditions, the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68±0.12%, which closely agreed with the predicted value (11.77%). After preliminary purification with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose, arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39 and its molecular weight was 1.49×10(4)kDa. The antioxidant activity of RNLP I was evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP I showed strong DPPH and superoxide radical scavenging activities and reducing power.
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Cai
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: yangyu_002@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Gaijie
authors.initials: G
authors.lastname: Liu
mesh_terms: D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D008970:Molecular Weight; D009005:Monosaccharides; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011312:Pressure; D031965:Ribes; D013055:Spectrophotometry, Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water
keywords: 1,1-Diphenyl-1-picrylhydrazyl (PubChem CID: 2735032); Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Blackcurrant; Chemical characterisation; Phenanthroline (PubChem CID: 1318); Polysaccharides; Pressurised water extraction; Pyrogallol (PubChem CID: 1057); Response surface methodology; Trifluoroacetic acid (PubChem CID: 6422); d-Galacturonic acid (PubChem CID: 84740); d-Glucose (PubChem CID: 5793)