title: Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles.
abstract: In the protein separation, adsorption and desorption of target protein have been using different buffer condition. Different buffer will change the structure and activity of target protein in some cases. This work describes the use of different wavelength light for remote regulation of adsorption and desorption of target protein in the same buffer solutions. A dynamic system that captured and released protein in response to light is reported. Matrix gold nanoparticles and light-responsive affinity ligand comprising thrombin aptamer (APT15), polyethylene glycol linker, and azobenzene-modified complementary sequence were used. UV light induced a trans-cis isomerization of the azobenzene that destabilized the duplex of aptamer and azobenzene-modified complementary sequence, resulting in thrombin binding to aptamer sequence. Visible light irradiation resulted in DNA duplex rehybridization and thrombin released. Our work demonstrates that different light wavelengths effectively regulated the adsorption and desorption of thrombin in the same buffer, and this system also can capture and release prothrombin from plasma with different wavelength light. Furthermore, this method can be widely applied to a variety of different protein separation process.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiemiao
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: lryang@ipe.ac.cn.
authors.forename: Liangrong
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: Liang
authors.affiliation: Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Graduate University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Dong
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China; Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: hzliu@ipe.ac.cn.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001391:Azo Compounds / Q000737:chemistry; D001483:Base Sequence; D002417:Cattle; D006046:Gold / Q000737:chemistry*; D007536:Isomerism; D053768:Metal Nanoparticles / Q000737:chemistry*; D011516:Prothrombin / Q000737:chemistry / Q000378:metabolism; D013917:Thrombin / Q000737:chemistry* / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D052157:Aptamers, Nucleotide; D001391:Azo Compounds; D006046:Gold; D011516:Prothrombin; D013917:Thrombin; C009850:azobenzene
keywords: Aptamer; Capture; Photoresponsive; Release; Thrombin