title: Modulation of keratin in adhesion, proliferation, adipogenic, and osteogenic differentiation of porcine adipose-derived stem cells.
abstract: Recently, keratin attracts tremendous interest because of its intrinsic ability to interact with different cells. It has the potential to serve as a controllable extracellular matrix protein that can be used to demonstrate cell mechanism and cell-matrix interaction. However, there have been relatively few studies on the effects of keratin on stem cells. In the present work, we study the effects of human keratin on porcine adipose-derived stem cells (pASCs) and a series of selective cell lines: 3T3 fibroblasts, Madin-Darby canine kidney (MDCK) cells, and MG63 osteoblasts. Relative to un-treated culture plate, our results showed that keratin coating substrates promote cell adhesion and proliferation to above cell lines. Keratin also improved pASCs adhesion, proliferation, and enhanced cell viability. Evaluation of genetic markers showed that adipogenic and osteogenic differentiations of pASCs can be successfully induced, thus demonstrating that keratin did not influence the stemness of pASCs. Furthermore, keratin improved adipogenic differentiations of pASCs in terms of up-regulations in lipoprotein lipase, peroxisome proliferator-activated receptor gamma, and CCAAT-enhancer-binding protein alpha. The osteogenic markers type I collagen, runt-related transcription factor 2, and vitamin D receptor were also upregulated when pASCs cultured on keratin substrates. Therefore, keratin can serve as a biological derived material for surface modification and scaffold fabrication for biomedical purpose. The combination of keratin with stem cells may be a potential candidate for tissue repair in the field of regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 180-192, 2017.
authors.affiliation: Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, 10617, Taiwan.
authors.forename: Yen-Lin
authors.initials: YL
authors.lastname: Wu
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, 10617, Taiwan.
authors.forename: Che-Wei
authors.initials: CW
authors.lastname: Lin
authors.affiliation: Department of Surgery, National Taiwan University Hospital, Taipei, 10031, Taiwan.
authors.forename: Nai-Chen
authors.initials: NC
authors.lastname: Cheng
authors.affiliation: School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, 11031, Taiwan.|Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, 11031, Taiwan.
authors.forename: Kai-Chiang
authors.initials: KC
authors.lastname: Yang
authors.affiliation: Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei, 10617, Taiwan.
authors.forename: Jiashing
authors.initials: J
authors.lastname: Yu
mesh_terms: D016475:3T3 Cells; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004285:Dogs; D007633:Keratins / Q000737:chemistry / Q000494:pharmacology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013552:Swine
chemical_list: D007633:Keratins
keywords: adhesion; differentiation; keratin; porcine adipose stem cell