title: [The anti-inflammatory effects of heme oxygenase-1 on emphysema model].
abstract: OBJECTIVE
  The effects of HO-1 on smoke-induced emphysema were tested and the mechanisms were explored in a smoking rat model.
  
  
  METHODS
  In this study, rats were either exposed or sham-exposed to cigarette smoke for emphysema modeling. Hemin were injected during this period to induce HO-1. Subsequently, emphysema development, inflammatory cells and cytokine levels were analyzed.
  
  
  RESULTS
  Smoke exposure induced emphysema [MLI: (34.0 ± 2.2) µm, MAN: 2.3 ± 0.5, MAA: (0.5 ± 0.1) µm²], increased the inflammatory cells count [total number: (41.0 ± 13.9) × 10⁴/ml, Neutrophil: (25.0 ± 9.8) × 10⁴/ml, Macrophage: (7.2 ± 2.8) × 10⁴/ml] and inflammatory cytokine levels especially IL-17 [serum: (33 ± 8.4) pg/ml, lung tissue homogenate: (79 ± 7) pg/ml, BALF: (39 ± 8) pg/ml], IL-8 [serum: (181 ± 51) pg/ml, BALF: (162 ± 79) pg/ml] and TNF-α [serum: (607 ± 85) pg/ml, BALF: (504 ± 223) pg/ml]. HO-1 reduced the severity of emphysema [MLI: (28.6 ± 1.1) µm, MAN: (2.7 ± 0.7), MAA: (0.4 ± 0.1) µm²]; total number: (17.6 ± 5.6) × 10²/ml, Neutrophil: (10.2 ± 3.6) × 10²/ml, Macrophage: (2.6 ± 1.1) × 10²/ml, suppressed the secretion of IL-17 [serum: (23.9 ± 2.4) pg/ml, lung tissue homogenate: (69.2 ± 3.0) pg/ml, BALF: (17.8 ± 5.3) pg/ml], IL-8 [serum:(103 ± 50) pg/ml, BALF: (114 ± 35) pg/ml] and TNF-α [serum: (423 ± 48) pg/ml, BALF: (216 ± 134) pg/ml] and prevented emphysema development.
  
  
  CONCLUSIONS
  HO-1 has anti-inflammatory effects and can prevent smoke-induced emphysema development.
authors.affiliation: Pediatrics Department, Shanxi Medical University, 030001, Pneumology Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wei
authors.affiliation: Pediatrics Department, Shanxi Medical University, 030001, Pneumology Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Pediatrics Department, Shanxi Medical University, 030001, Pneumology Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, China; Email: ljqhx@sina.com.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D016207:Cytokines; D004646:Emphysema; D051547:Heme Oxygenase-1; D020381:Interleukin-17; D016209:Interleukin-8; D008168:Lung; D008264:Macrophages; D009504:Neutrophils; D011656:Pulmonary Emphysema; D051381:Rats; D012906:Smoke; D012907:Smoking; D014026:Nicotiana; D014409:Tumor Necrosis Factor-alpha
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D016209:Interleukin-8; D012906:Smoke; D014409:Tumor Necrosis Factor-alpha; D051547:Heme Oxygenase-1