title: Bioactivity of Essential Oil of Zingiber purpureum Rhizomes and Its Main Compounds against Two Stored Product Insects.
abstract: The insecticidal and repellent activities of the essential oil extracted from Zingiber purpureum Roscoe rhizomes were evaluated against Tribolium castaneum (Herbst) and Lasioderma serricorne (L.) adults. During our screening program for agrochemicals from Chinese medicinal herbs and wild plants, the essential oil of Z. purpureum rhizomes was found to possess strong contact toxicity against T. castaneum and L. serricorne adults, with LD50 values of 39.0 and 16.3 µg per adult, respectively, and also showed strong fumigant toxicity against the two grain storage insects with LC50 values of 13.6 and 9.3 mg/liter of air, respectively. The essential oil obtained by hydrodistillation was investigated by gas chromatography-mass spectrometry. The main components of the essential oil were identified to be sabinene (48.1%), terpinen-4-ol (25.1%), and γ-terpinene (6.7%), followed by α-terpinene (4.3%), β-thujene (3.4%), and α-phellandrene (2.7%). Sabinene, terpinen-4-ol, and γ-terpinene were separated and purified by silica gel column chromatography and preparative thin-layer chromatography. Terpinen-4-ol showed the strongest contact toxicity against T. castaneum and L. serricorne (LD50=19.7 and 5.4 µg per adult, respectively) and also the strongest fumigant toxicity against T. castaneum and L. serricorne (LC50=3.7 and 1.3 mg/liter of air, respectively). Otherwise, sabinene and terpinen-4-ol were strongly repellent against T. castaneum as well as the essential oil, while γ-terpinene exhibited weaker repellency against T. castaneum compared with the positive control, DEET (N, N-diethyl-3-methylbenzamide). Moreover, only the essential oil exhibited strong repellency against L. serricorne, the three compounds exhibited weaker repellency against L. serricorne relative to DEET.
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700.
authors.forename: C X
authors.initials: CX
authors.lastname: You
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700.
authors.forename: K
authors.initials: K
authors.lastname: Yang
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. Technical Center of China Tobacco Guangxi Industrial Co., Ltd., Nanning, China, 530001.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700.
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700.
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Department of Entomology, China Agricultural University, Haidian District, Beijing, China, 100193.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijng, China, 100700. dushushan@bnu.edu.cn.
authors.forename: S S
authors.initials: SS
authors.lastname: Du
authors.affiliation: Analytical and Testing Center, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Deng
authors.affiliation: Analytical and Testing Center, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Geng
authors.affiliation: Analytical and Testing Center, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, China,100875.
authors.forename: J
authors.initials: J
authors.lastname: Han
mesh_terms: D000818:Animals; D001517:Coleoptera; D007306:Insecticides; D009822:Oils, Volatile; D010936:Plant Extracts / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D007306:Insecticides; D009822:Oils, Volatile; D010936:Plant Extracts
keywords: Lasioderma serricorne; Tribolium castaneum; Zingiber purpureum; insecticidal activity; repellency