title: A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system.
abstract: We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min(-1) and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea. ahjeong.son@gmail.com.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea. ahjeong.son@gmail.com.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Lim
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea. ahjeong.son@gmail.com.
authors.forename: Ahjeong
authors.initials: A
authors.lastname: Son
authors.affiliation: School of Electrical Engineering, Korea University, Seoul, Republic of Korea. chuabeelee@gmail.com.
authors.forename: Beelee
authors.initials: B
authors.lastname: Chua
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification*; D058491:Bacterial Load / Q000295:instrumentation*; D004867:Equipment Design; D010126:Ozone / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D012967:Sodium Dodecyl Sulfate; D010126:Ozone