title: Evaluation of feed grade sodium bisulfate impact on gastrointestinal tract microbiota ecology in broilers via a pyrosequencing platform.
abstract: The gastrointestinal microbial community in broiler chickens consists of many different species of bacteria, and the overall microbiota can vary from bird to bird. To control pathogenic bacteria in broilers and improve gut health, numerous potential dietary amendments have been used. In this study, we used a pyrosequencing platform to evaluate the effect of sodium bisulfate on microbiota of the crop, cecum, and ileum of broiler chickens grown over several weeks. The diversity information in each digestive organ sample exhibited considerable variation and was clearly separable, suggesting distinct bacterial populations. Although no apparent microbial clustering occurred between the control and the dietary treatments, we did observe shifts in overall microbiota populations in the crop, ileum, and ceca as well as changes in specific microorganisms such as Bacteroides, Clostridium, and Lactobacillus species that were identified as birds became older.
authors.affiliation: Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville.
authors.forename: Si Hong
authors.initials: SH
authors.lastname: Park
authors.affiliation: MR DNA (Molecular Research LP), Shallowater, TX.
authors.forename: Scot E
authors.initials: SE
authors.lastname: Dowd
authors.affiliation: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA, Agricultural Research Service, College Station, TX.
authors.forename: Jack L
authors.initials: JL
authors.lastname: McReynolds
authors.affiliation: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA, Agricultural Research Service, College Station, TX.
authors.forename: James A
authors.initials: JA
authors.lastname: Byrd
authors.affiliation: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, USDA, Agricultural Research Service, College Station, TX.
authors.forename: David J
authors.initials: DJ
authors.lastname: Nisbet
authors.affiliation: Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville sricke@uark.edu.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002432:Cecum / Q000187:drug effects / Q000382:microbiology; D002645:Chickens / Q000378:metabolism* / Q000382:microbiology; D003426:Crop, Avian / Q000187:drug effects / Q000382:microbiology; D004269:DNA, Bacterial; D004032:Diet / Q000662:veterinary; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D007082:Ileum / Q000187:drug effects / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; C012036:sodium sulfate
keywords: broiler chicken; microbiota; pyrosequencing; sodium bisulfate