title: CaNa2EDTA chelation attenuates cell damage in workers exposed to lead--a pilot study.
abstract: Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa2EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa2EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 ± 1.2 years were administered five-day CaNa2EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDH1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa2EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. Electronic address: andrea@pharmacy.bg.ac.rs.
authors.forename: A
authors.initials: A
authors.lastname: Čabarkapa
authors.affiliation: Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia. Electronic address: sborozan@vet.bg.ac.rs.
authors.forename: S
authors.initials: S
authors.lastname: Borozan
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. Electronic address: lada@pharmacy.bg.ac.rs.
authors.forename: L
authors.initials: L
authors.lastname: Živković
authors.affiliation: ICTM Department of Chemistry, University of Belgrade, Belgrade, Serbia. Electronic address: srdjanst@chem.bg.ac.rs.
authors.forename: S
authors.initials: S
authors.lastname: Stojanović
authors.affiliation: Institute of Occupational Health of Serbia "Dr Dragomir Karajović", Belgrade, Serbia. Electronic address: mirmilcab@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Milanović-Čabarkapa
authors.affiliation: Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research "Vinca", University of Belgrade, Serbia. Electronic address: vladanbajic@gmail.com.
authors.forename: V
authors.initials: V
authors.lastname: Bajić
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. Electronic address: bilja22@pharmacy.bg.ac.rs.
authors.forename: B
authors.initials: B
authors.lastname: Spremo-Potparević
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D043303:Aryldialkylphosphatase / Q000097:blood; D002614:Chelating Agents / Q000494:pharmacology*; D015913:Chelation Therapy / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007854:Lead / Q000097:blood / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D010865:Pilot Projects
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D007527:Isoenzymes; D007854:Lead; D004492:Edetic Acid; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: CaNa(2)EDTA chelation; Genotoxicity; Lead exposure; Oxidative damage; Trace elements