title: Simultaneous detection of multiple mycotoxins in broiler feeds using a liquid chromatography tandem-mass spectrometry.
abstract: Mycotoxins are secondary fungal metabolites that are typically present in grain and feed ingredients used for animal feeds. An analytical method using LC-ESI-MS/MS was developed to quantify nine mycotoxins, consisting of aflatoxin B1 (AFB1), AFB2, AFG1, AFG2, T-2 toxin, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA) and ochratoxin A (OTA) in broiler feeds. In total, 100 samples of broiler feeds were collected from poultry farms in Central Thailand. The survey found that AFB1 and ZEA were the most prevalent mycotoxins in the feed samples at percentages of 93% and 63%, respectively. The limit of detections (LODs) of investigated mycotoxins was 0.20-0.78 ng/g. AFB2, DON, AFG1, NIV and T-2 toxin were also detectable at low contamination levels with percentages of 20%, 9%, 7%, 5% and 1%, respectively, whereas OTA and AFG2 were not detected in any of the feed samples. These results suggest that there is a very low level of risk of the exposure to mycotoxins in feeds obtained from broiler farms in Central Thailand.
authors.affiliation: Interdisciplinary Graduate Program in Agricultural Biotechnology, Graduate School, Kasetsart University, KamphaengSaen Campus, Nakhon, Pathom 73140, Thailand.
authors.forename: Jutamart
authors.initials: J
authors.lastname: Kongkapan
authors.forename: Saranya
authors.initials: S
authors.lastname: Poapolathep
authors.forename: Supaporn
authors.initials: S
authors.lastname: Isariyodom
authors.forename: Susumu
authors.initials: S
authors.lastname: Kumagai
authors.forename: Amnart
authors.initials: A
authors.lastname: Poapolathep
mesh_terms: D000383:Agriculture; D000821:Animal Feed; D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013785:Thailand
chemical_list: D009183:Mycotoxins