title: Visualization of cancer-related chemical components in mouse pancreas tissue by tapping-mode scanning probe electrospray ionization mass spectrometry.
abstract: Mass spectrometry imaging is an informative approach for the comprehensive analysis of multiple components inside biological specimens. We used novel tapping-mode scanning probe electrospray ionization mass spectrometry method to visualize cancer-related chemical components in the mouse pancreas tissue section at a sampling pitch of 100 µm. Positive ion mode measurements from m/z 100 to 1500 resulted in the visualization of multiple components that are tentatively assigned as polyamines, lipids and proteins. Their signal intensities inside the cancerous and the non-cancerous regions were found to be significantly different by the two-sample t-test.
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan.|Department of Chemistry, Graduate School of Science, Osaka University, 1-1, Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Otsuka
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan.
authors.forename: Shuya
authors.initials: S
authors.lastname: Satoh
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Naito
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Kyogaku
authors.affiliation: Frontier Research Center, Canon Inc., 30-2 Shimomaruko 3-chome, Ohta-ku, Tokyo, 146-8501, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D010179:Pancreas / Q000737:chemistry*; D010190:Pancreatic Neoplasms / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor
keywords: ambient ionization; biomarker discovery; cancer tissues; cancer-related proteins; mass spectrometry imaging