title: Theranostic, pH-Responsive, Doxorubicin-Loaded Nanoparticles Inducing Active Targeting and Apoptosis for Advanced Gastric Cancer.
abstract: This study developed a kind of magnetic-polymer nanocarrier with folate receptor-targeting and pH-sensitive multifunctionalities to carry doxorubicin (DOX) for treatment of advanced gastric cancer (AGC). Folate-conjugated, pH-sensitive, amphiphilic poly(β-aminoester) self-assembled with hydrophobic oleic acid-modified iron oxide nanoparticles, and the resulting hydrophobic interaction area is a reservoir for lipophilic DOX (F-P-DOX). Confocal microscopy illustrated that F-P-DOX treatment could keep higher DOX accumulation in cells than P-DOX (without folate conjugation), and therefore get a higher efficiency of DOX internalization at pH 6.5 than at pH 7.4. Electron microscope characterization and real-time polymerase chain reaction revealed cell apoptosis promoted by F-P-DOX. The better efficacy of F-P-DOX on GC than free DOX and P-DOX was determined by MTT assay and xenograft model. Moreover, the accumulation of F-P-DOX in the tumor site was detected by magnetic resonance imaging (MRI). All those observations suggest F-P-DOX could be a promising theranostic candidate for AGC treatment.
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University , Guangzhou 510515, China.
authors.forename: Huanrong
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Mechanical Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Children's Hospital Research Institute of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University , Guangzhou 510515, China.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Mechanical Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Shao
authors.affiliation: Department of Biosystem Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Oncology, Nanfang Hospital, Southern Medical University , Guangzhou 510515, China.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Mechanical Engineering, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Department of Biochemistry and Medical Genetics, University of Manitoba , Winnipeg Manitoba R3T 2N2, Canada.|Children's Hospital Research Institute of Manitoba , Winnipeg, Manitoba R3E 3P4, Canada.
authors.forename: Malcolm M Q
authors.initials: MM
authors.lastname: Xing
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000628:therapy*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D018450:Disease Progression; D004317:Doxorubicin / Q000494:pharmacology*; D004337:Drug Carriers; D065546:Drug Liberation; D005290:Ferric Compounds / Q000737:chemistry; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D059346:Magnets; D008297:Male; D051379:Mice; D008819:Mice, Nude; D019301:Oleic Acid / Q000737:chemistry; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*; D000068936:Theranostic Nanomedicine / Q000379:methods*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D019301:Oleic Acid; D004317:Doxorubicin; D005492:Folic Acid