title: Outbreak of chickenpox in a Union Territory of North India.
abstract: OBJECTIVE
  Primary infection with a varicella-zoster virus (VZV) leads to chickenpox. Though the incidence of the disease has decreased in many developed countries due to the introduction of the varicella vaccine, outbreaks continue to occur in developing countries.
  
  
  METHODS
  The present study reports an outbreak of varicella in an urbanised village in the vicinity of Chandigarh City in North India in November 2013. The outbreak was confirmed by the detection of VZV IgM antibodies in serum samples of clinically suspected patients. Vesicular fluid samples were collected from 8 patients with active lesions and tested for VZV DNA by polymerase chain reaction. Blood samples were also collected from 17 healthy controls residing in the same locality and tested for the presence of VZV IgM and IgG antibodies.
  
  
  RESULTS
  A total of 18 cases occurred, and the majority of them (67%) were <15 years of age. Of 17 samples collected from patients with the clinically suspected disease, 13 (76.5%) showed the presence of VZV IgM antibodies. Of the healthy controls, 6 were VZV IgM positive and 4 of them developed symptomatic disease on follow-up. VZV DNA was positive in 5/8 (62.5%) of the patients. In one patient, VZV DNA was detected in the absence of an IgM antibody response.
  
  
  CONCLUSIONS
  The introduction of varicella vaccine in the universal immunisation programme of India may help to prevent these outbreaks; however, the cost-benefit analysis needs to be carried out before making such policies.
authors.affiliation: Department of Virology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: M P
authors.initials: MP
authors.lastname: Singh
authors.forename: C
authors.initials: C
authors.lastname: Chandran
authors.forename: A
authors.initials: A
authors.lastname: Sarwa
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Gupta
authors.forename: A
authors.initials: A
authors.lastname: Raj
authors.forename: R K
authors.initials: RK
authors.lastname: Ratho
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002644:Chickenpox / Q000453:epidemiology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007194:India / Q000453:epidemiology; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M