title: Differential proteomics to explore the inhibitory effects of acidic, slightly acidic electrolysed water and sodium hypochlorite solution on Vibrio parahaemolyticus.
abstract: Slightly acidic electrolysed water (SlAEW) and acidic electrolysed water (AEW) have been demonstrated to effectively inactivate food-borne pathogens. However, the underlying mechanism of inactivation remains unknown. Therefore, in this study, a differential proteomic platform was used to investigate the bactericidal mechanism of SlAEW, AEW, and sodium hypochlorite (NaOCl) solutions against Vibrio parahaemolyticus. The upregulated proteins after SlAEW, AEW, and NaOCl treatments were identified as outer membrane proteins K and U. The downregulated proteins after the SlAEW, AEW, and NaOCl treatments were identified as adenylate kinase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and enolase, all of which are responsible for energy metabolism. Protein synthesis-associated proteins were downregulated and identified as elongation factor Tu and GAPDH. The inhibitory effects of SlAEW and AEW solutions against V. parahaemolyticus may be attributed to the changes in cell membrane permeability, protein synthesis activity, and adenosine triphosphate (ATP) biosynthesis pathways such as glycolysis and ATP replenishment.
authors.affiliation: Department of Food Science, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan, ROC. Electronic address: tychen@mail.ntou.edu.tw.
authors.forename: Tai-Yuan
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Department of Food Science, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.
authors.forename: Shu-Hao
authors.initials: SH
authors.lastname: Kuo
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, ROC.
authors.forename: Shui-Tein
authors.initials: ST
authors.lastname: Chen
authors.affiliation: Department of Food Science, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202, Taiwan, ROC.
authors.forename: Deng-Fwu
authors.initials: DF
authors.lastname: Hwang
mesh_terms: D004572:Electrolysis; D040901:Proteomics / Q000379:methods*; D012973:Sodium Hypochlorite / Q000378:metabolism*; D014736:Vibrio parahaemolyticus / Q000737:chemistry*; D014867:Water / Q000378:metabolism*
chemical_list: D014867:Water; D012973:Sodium Hypochlorite
keywords: Acidic electrolysed water; Bactericidal mechanism; Proteomic; Slightly acidic electrolysed water; V. parahaemolyticus