title: Improving blood-compatibility via surface heparin-immobilization based on a liquid crystalline matrix.
abstract: Blood compatibility is of considerable importance in developing medical materials and devices that are in contact with blood. In this work, we successfully developed a novel liquid crystalline heparin-immobilized material (Hep-OPPC) by two-step modification for further improvement of hydrophilicity and hemocompatibility of the liquid crystalline hydroxypropyl cellulose ester (OPCL). The results showed that Hep-immobilization on the OPCL led to dramatic changes in the surface morphology and crystallinity, whereas, the Hep-OPPCs also maintained the liquid crystalline feature at room temperature after heparinization. Furthermore, the hemocompatibility of the Hep-OPPCs was markedly enhanced at low levels of hemolysis assay (HR) with unimpaired erythrocytomorphology, significantly lower concentrations of C3a in blood plasma and remarkable increases in plasma re-calcification time (PRT). This suggests that the heparinized surface could restrict the transformation of fibrinogen with less activation of the intrinsic coagulation system. Moreover, the activated partial thromboplastin time (APTT) and prothrombin time (PT) values of the Hep-OPPCs with low heparin density could also be prolonged in this study suggesting that the liquid crystal feature of the matrix might be blocking the clotting factors. We concluded that the heparin-immobilized liquid crystalline material has the potential to be used in blood-contact materials.
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Jupeng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Shenyu
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: The First Affiliated Hospital, Jinan University, Guangzhou 510632, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Medicine, Jinan University, Guangzhou 510632, PR China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital, Jinan University, Guangzhou 510632, PR China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Zha
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China. Electronic address: tumei@jnu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Tu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D003167:Complement Activation / Q000187:drug effects; D004903:Erythrocyte Aggregation / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000737:chemistry*; D050866:Liquid Crystals / Q000737:chemistry*; D010973:Platelet Adhesiveness / Q000187:drug effects; D010979:Platelet Function Tests; D011817:Rabbits; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D006493:Heparin
keywords: Blood compatibility; Heparin; Liquid crystal; Surface modification