title: The Association between Human Leukocyte Antigen Class II DR3-DQ2 Haplotype and Type 1 Diabetes in Children of the East Azerbaijan State of Iran.
abstract: BACKGROUND
  Type 1 diabetes mellitus (T1D) is an autoimmune disease. Several associations between human leukocyte antigen (HLA) complex and T1D were found in various populations. Associations with various HLA types depend on the investigated populations. However, such associations have not yet been investigated in the East Azerbaijan state of Iran with Turkish ethnicity.
  
  
  OBJECTIVE
  The aims of the current study was to describe T1D genetic susceptibility conferred by HLA class II alleles (DRB1*0301, DQA1*0501 and DQB1*0201) and to determine haplotype frequencies among T1D patients.
  
  
  METHODS
  This study was a case-control study. The number of samples was determined using the Cochran formula. Eighty unrelated T1D patients, including 42 (52.5%) females and 38 (47.5%) males, were randomly recruited from the East Azerbaijan state of Iran. Typing of HLA was performed by polymerase chain reaction-sequence-specific priming (PCR-SSP) on DNA extracted from peripheral blood mononuclear cells of 80 unrelated patients and 80 unrelated healthy control donors, who were selected randomly. For haplotype analysis, the logistic regression model was performed that allows joint estimation of Single-nucleotide polymorphisms (SNPs) via haplotypes.
  
  
  RESULTS
  The frequency of drb1*0301 (82.5% vs. 11.3%), dqa1*0501 (82.5% vs. 36.3%) and dqb1*0201 (81.3% vs. 35%) were significantly higher among patients compared with that of healthy subjects.
  
  
  CONCLUSIONS
  Our investigation demonstrated that there is a highly significant association between the studied alleles and T1D. It can be construed that haplotype HLA-DR3-DQ2 has a very modest effect with respect to the risk of T1D.
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Mansoori Derakhshan
authors.affiliation: Department of Biological Science, Ahar Branch, Islamic Azad University, Ahar, IR Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Zeinali Sehrig
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Sohrabi
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Siamak
authors.initials: S
authors.lastname: Shiva
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Baradaran
authors.affiliation: Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Shekari Khaniani
keywords: Genotype; HLA-DQB1; HLA-DRB1; Haplotype