title: Disseminated mycobacteriosis manifesting as paraplegia in two Parma wallabies (Macropus parma) naturally exposed to Mycobacterium avium.
abstract: Two captive female Parma wallabies (Macropus parma) died after a history of flaccid paraplegia. On postmortem examination, granulomatous and suppurative osteomyelitis involving the left ischium and the lumbosacral region, with meningeal extension at the cauda equina, and caseonecrotic mastitis were the most significant changes. Multiple small nodules in the liver and spleen, and an enlargement of some lymph nodes with central caseous necrosis were also observed. Microscopically, a disseminated granulomatous inflammation with numerous multinucleate giant cells was seen. Numerous acid-fast bacilli were detected in macrophages, in multinucleated giant cells, and free in the central necrosis and suppurative exudate. After culture, polymerase chain reaction assays were carried out to detect the 65-kDa heat shock protein (Hsp65) and insertion sequences (IS)1245 and IS900. The causative agent was identified as Mycobacterium avium subsp. avium.
authors.affiliation: Department of Pathology (Robveille, Gaide, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, FranceAnimal Models for Comparative Oncology Research (AMaROC; Albaric, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, France jerome.abadie@oniris-nantes.fr.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Robveille
authors.affiliation: Department of Pathology (Robveille, Gaide, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, FranceAnimal Models for Comparative Oncology Research (AMaROC; Albaric, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Albaric
authors.affiliation: Department of Pathology (Robveille, Gaide, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, FranceAnimal Models for Comparative Oncology Research (AMaROC; Albaric, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gaide
authors.affiliation: Department of Pathology (Robveille, Gaide, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, FranceAnimal Models for Comparative Oncology Research (AMaROC; Albaric, Abadie), Nantes-Atlantic College of Veterinary Medicine and Food Sciences (ONIRIS), LUNAM University, Site de la Chantrerie, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Abadie
mesh_terms: D000818:Animals; D005260:Female; D005602:France; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D007614:Macropodidae; D009162:Mycobacterium avium / Q000302:isolation & purification*; D010264:Paraplegia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013154:Spleen / Q000473:pathology; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
keywords: Granulomatous mastitis; Mycobacterium avium; osteomyelitis; paralysis; wallaby