title: Inactivation of Fam20B in the dental epithelium of mice leads to supernumerary incisors.
abstract: Tooth formation is tightly regulated by epithelial-mesenchymal interactions via hierarchic cascades of signaling molecules. The glycosaminoglycan (GAG) chains covalently attached to the core protein of proteoglycans (PGs) provide docking sites for signaling molecules and their receptors during the morphogenesis of tissues and organs. Although PGs are believed to play important roles in tooth formation, little is known about their exact functions in this developmental process and the relevant molecular basis. Family with sequence similarity member 20-B (FAM20B) is a newly identified kinase that phosphorylates the xylose in the common linkage region connecting the GAG with the protein core of PGs. The phosphorylation of xylose is essential for elongation of the common linkage region and the subsequent GAG assembly. In this study, we generated a Fam20B-floxed allele in mice and found that inactivating Fam20B in the dental epithelium leads to supernumerary maxillary and mandibular incisors. This finding highlights the pivotal role of PGs in tooth morphogenesis and opens a new window for understanding the regulatory mechanism of PG-mediated signaling cascades during tooth formation.
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.|Department of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Pan
authors.initials: P
authors.lastname: Ma
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Xiudong
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Derrick M
authors.initials: DM
authors.lastname: Crawford
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX, USA.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D004848:Epithelium; D007180:Incisor; D051379:Mice; D009805:Odontogenesis; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor); D011506:Proteins / Q000378:metabolism*; D011509:Proteoglycans
chemical_list: C509695:FAM20B protein, mouse; D011506:Proteins; D011509:Proteoglycans; D017853:Phosphotransferases (Alcohol Group Acceptor)
keywords: glycosaminoglycan; kinase; proteoglycan; supernumerary teeth; tooth development