title: A Microplate-Based System as In Vitro Model of Biofilm Growth and Quantification.
abstract: We describe a 96-well microtiter plate-based system as an in vitro model for biofilm formation and quantification. Although in vitro assays are artificial systems and thus significantly differ from in vivo conditions, they represent an important tool to evaluate biofilm formation and the effect of compounds on biofilms. Stainings to evaluate the amount of biomass (crystal violet staining) and the number of metabolically active cells (resazurin assay) are discussed and specific attention is paid to the use of this model to quantify persisters in sessile populations.
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Vandecandelaere
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.
authors.forename: Heleen
authors.initials: H
authors.lastname: Van Acker
authors.affiliation: Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium. Tom.Coenye@UGent.be.
authors.forename: Tom
authors.initials: T
authors.lastname: Coenye
mesh_terms: D001422:Bacterial Adhesion / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development*; D018929:Cell Culture Techniques / Q000379:methods*; D005840:Gentian Violet / Q000494:pharmacology; D010078:Oxazines / Q000494:pharmacology; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D005840:Gentian Violet
keywords: Biofilms; Crystal violet staining; In vitro model system; Microtiter plate; Persisters; Resazurin assay