title: Lysosome-associated membrane glycoprotein (LAMP)--preliminary study on a hidden antigen target for vaccination against schistosomiasis.
abstract: Our previously reported gene atlasing of schistosome tissues revealed transcripts that were highly enriched in the digestive tract of Schistosoma mansoni. From these, we selected two candidates, Sm-LAMP and Sm-NPC2 for testing as vaccine targets. The two molecules were selected on the basis of relatively high expression in the gastrodermis, their potentially important biological function, divergence from homologous molecules of the host and possible apical membrane expression in the gastrodermis. Bacterially expressed recombinant peptides corresponding to regions excluding trans-membrane domains of the selected vaccine targets were used in blinded vaccine trials in CBA mice using alum-CpG as adjuvant. Vaccine trials using the recombinant insoluble Sm-LAMP protein showed 16-25% significant reduction in total worm burden. Faecal egg count reduction was 52% and 60% in two trials, respectively, with similar results for the solubly expressed protein. Liver egg burden was reduced significantly (20% and 38%) with an insoluble recombinant Sm-LAMP in two trials, but not with the soluble recombinant form. Parasite fecundity was not affected by either Sm-LAMP protein preparations in the trials. It is concluded that Sm-LAMP may provide limited protection towards S. mansoni infections but could be used in combination with other vaccine candidates, to provide more comprehensive protection.
authors.affiliation: School of Veterinary Sciences, The University of Queensland, Gatton Campus, Gatton Qld, 4343, Australia.|QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Sujeevi S K
authors.initials: SS
authors.lastname: Nawaratna
authors.affiliation: QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Geoffrey N
authors.initials: GN
authors.lastname: Gobert
authors.affiliation: QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Charlene
authors.initials: C
authors.lastname: Willis
authors.affiliation: QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Jason
authors.initials: J
authors.lastname: Mulvenna
authors.affiliation: Structural Chemistry Program, Eskitis Institute, Griffith University, Brisbane, Qld 4111, Australia.|Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hofmann
authors.affiliation: QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Donald P
authors.initials: DP
authors.lastname: McManus
authors.affiliation: School of Veterinary Sciences, The University of Queensland, Gatton Campus, Gatton Qld, 4343, Australia.|QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, Qld, 4006, Australia.
authors.forename: Malcolm K
authors.initials: MK
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D051907:Lysosomal Membrane Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D059208:Parasite Load; D010802:Phylogeny; D021381:Protein Transport; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000469:parasitology / Q000517:prevention & control*
chemical_list: D000947:Antigens, Helminth; D051907:Lysosomal Membrane Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins