title: Biosensor for label-free DNA quantification based on functionalized LPGs.
abstract: A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.
authors.affiliation: University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal.
authors.forename: Helena M R
authors.initials: HM
authors.lastname: Gonçalves
authors.affiliation: University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal.
authors.forename: Luis
authors.initials: L
authors.lastname: Moreira
authors.affiliation: University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI- Biosystems & Integrative Sciences Institute, Campo Grande, Lisboa, Portugal.
authors.forename: Leonor
authors.initials: L
authors.lastname: Pereira
authors.affiliation: INESC TEC, Rua do Campo Alegre n. 687, 4169-007 Porto, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Jorge
authors.affiliation: INESC TEC, Rua do Campo Alegre n. 687, 4169-007 Porto, Portugal.
authors.forename: Carlos
authors.initials: C
authors.lastname: Gouveia
authors.affiliation: University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI- Biosystems & Integrative Sciences Institute, Campo Grande, Lisboa, Portugal.
authors.forename: Paula
authors.initials: P
authors.lastname: Martins-Lopes
authors.affiliation: University of Trás-os-Montes and Alto Douro, P.O. Box 1013, 5000-911 Vila Real, Portugal; INESC TEC, Rua do Campo Alegre n. 687, 4169-007 Porto, Portugal. Electronic address: jraf@utad.pt.
authors.forename: José R A
authors.initials: JR
authors.lastname: Fernandes
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods; D057230:Limit of Detection; D008958:Models, Molecular
chemical_list: D004247:DNA
keywords: Biosensing; DNA; Detection; Label-free; Quantification