title: [Expression of osteopontin splice variant and its clinical significance in gastric cancer].
abstract: OBJECTIVE
  To investigate the expression of osteopontin (OPN) splice variant mRNA, including the three isoforms OPN-A, OPN-B, and OPN-C, to explore its correlation with clinicopathologic features in gastric cancer, and to elucidate their role in tumor invasion and distant metastasis of gastric cancer.
  
  
  METHODS
  The expression of OPN-A, OPN-B and OPN-C mRNA were detected by real-time reverse transcriptase-polymerase chain reaction in 66 gastric cancer tissues. The relationship between the expression of OPN-A, OPN-B and OPN-C mRNA and clinicopathologic features of gastric cancer was analyzed.
  
  
  RESULTS
  The expression of OPN-C mRNA in the gastric cancer tissue was 3.21-fold higher than that in peritumoral mucosal tissue, showing a significant difference between them (P < 0.001). OPN-C mRNA expression was correlated with the depth of tumor invasion, tumor diameter, lymph node meatastasis, distant meatastasis and had no correlation with differentiation grades. The low expression of OPN-C mRNA was correlated with long survival benefit (P = 0.03). The expression of OPN-A and OPN-B mRNA had no significant relationship with clinicopathologic features of gastric cancer.
  
  
  CONCLUSIONS
  One of the isoform of osteopontin (OPN) OPN-C mRNA is overexpressed in gastric cancer. The overexpression of OPN-C mRNA may reflect the progression and is associated with the prognosis of gastric cancer. OPN-C mRNA may have value as a prognostic biomarker in gastric cancer. However, the expression of OPN-A and OPN-B are not correlated with the progression and metastasis of gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Shandong Tumor Hospital, Jinan 250117, China.
authors.forename: Xianjun
authors.initials: X
authors.lastname: Sun
authors.forename: Longgang
authors.initials: L
authors.lastname: Wang
authors.forename: Wenhong
authors.initials: W
authors.lastname: Hou
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Li
authors.forename: Liqing
authors.initials: L
authors.lastname: Liu
authors.forename: Wenshu
authors.initials: W
authors.lastname: Zuo
authors.affiliation: Email: sdyujinming@126.com.
authors.forename: Jinming
authors.initials: J
authors.lastname: Yu
mesh_terms: D018450:Disease Progression; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008198:Lymph Nodes; D008207:Lymphatic Metastasis; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics*; D053495:Osteopontin / Q000235:genetics*; D011379:Prognosis; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
chemical_list: D009363:Neoplasm Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D053495:Osteopontin