title: A clinicopathological study of peripheral lymph nodes in HIV-infected patients with special reference to CD4+ T-cell counts: Experience from a tertiary care institution in Darjeeling (India).
abstract: BACKGROUND
  HIV/AIDS is a major health burden worldwide. India bears the third highest HIV-patients load globally. In the Darjeeling district, HIV-prevalence is >1% with very little known about the profile of HIV-lymphadenopathy. The aim of this study was to identify the different causes of peripheral lymphadenopathy among HIV-infected patients in this region, correlate them with CD4+ T-cell counts and formulate some common clinico-haematological parameters as potential predictors of CD4+ T-cell count.
  
  
  METHODS
  In the present study, 76 cases were evaluated. Fine Needle Aspiration Cytology (FNAC) was performed as an out-patient procedure in the Department of Pathology. Smears were stained routinely with Haematoxylin-Eosin and Leishman stains. ZN stains were done when indicated by the cytological findings. Immediate CD4+ T-cell count was obtained by referring the patients to the Anti-retroviral therapy centre.
  
  
  RESULTS
  Cytological diagnoses included tuberculosis (82.9%), reactive hyperplasia (6.6%), nonspecific granulomatous lesions (3.9%), non-Hodgkin lymphoma (2.6%), histoplasmosis (2.6%) and simultaneous filariasis with toxoplasmosis (1.3%). Statistically, the opportunistic infections and lymphomas significantly concurred with a CD4+ T-cell count <350/μl. Likewise, the number of enlarged lymph nodes and absolute lymphocyte count (ALC) were found to be useful predictors of CD4+ T-cell counts.
  
  
  CONCLUSIONS
  Lymph node cytology in HIV-infected patients is essential to identify opportunistic infections from neoplastic lesions and; to enable therapeutic strategies. Correlation of lesions with mean CD4+ T-cell count predicts personal immunity, stage of disease and disease activity. Furthermore, enlarged lymph node numbers and ALC can be surrogate markers of CD4+ T-cell count for monitoring the severity of the immune suppression in under-resourced countries like India.
authors.affiliation: Department of Pathology, North Bengal Medical College & Hospital, India.
authors.forename: Rupali
authors.initials: R
authors.lastname: Mandal
authors.affiliation: Department of Pathology, North Bengal Medical College & Hospital, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Mondal
authors.affiliation: Department of General Medicine, North Bengal Medical College & Hospital, India.
authors.forename: Saikat
authors.initials: S
authors.lastname: Datta
authors.affiliation: Department of Pathology, North Bengal Medical College & Hospital, India.
authors.forename: Indranil
authors.initials: I
authors.lastname: Chakrabarti
authors.affiliation: Department of Pathology, North Bengal Medical College & Hospital, India.
authors.forename: Amita
authors.initials: A
authors.lastname: Giri
authors.affiliation: Department of Pathology, North Bengal Medical College & Hospital, India.
authors.forename: Bidyut Krishna
authors.initials: BK
authors.lastname: Goswami
mesh_terms: D000386:AIDS-Related Complex / Q000473:pathology*; D017088:AIDS-Related Opportunistic Infections / Q000473:pathology*; D018791:CD4 Lymphocyte Count; D005260:Female; D006801:Humans; D007194:India; D008198:Lymph Nodes / Q000473:pathology*; D008206:Lymphatic Diseases / Q000473:pathology*; D008297:Male; D062606:Tertiary Care Centers
keywords: CD4+ T-cell count; FNAC; HIV; Peripheral lymphadenopathy; absolute lymphocyte count