title: Ultrasound-Assisted Extraction of Pristimerin from Celastrus orbiculatus Using Response Surface Methodology.
abstract: This paper describes the ultrasound-assisted extraction (UAE) of pristimerin from Celastrus orbiculatus. Methanol was the most effective for pristimerin extraction, followed by ethanol, ethyl acetate, n-butanol, and water. To optimize the conditions, the Box-Behnken design, a widely used form of response surface methodology, was used to investigate the effects of parameters on UAE. Several variables, such as extraction time, ultrasonic power, extraction temperature, and solvent-to-solid (S/S) ratio were investigated. The highest extraction yield of 1.843 mg/g was obtained using methanol under optimal conditions with an extraction time of 40 min, ultrasonic power of 105 W, an S/S ratio of 40 mL/g, and an extraction temperature of 52°C. The experimental values under optimal conditions agreed well with the predicted values, suggesting that UAE has good potential as an extraction method for pristimerin from C. orbiculatus.
authors.affiliation: College of Pharmacy, Guangdong Pharmaceutical University.
authors.forename: Gong
authors.initials: G
authors.lastname: Fang
authors.forename: Guocheng
authors.initials: G
authors.lastname: Li
authors.forename: Chaohai
authors.initials: C
authors.lastname: Pang
authors.forename: Wenxi
authors.initials: W
authors.lastname: Li
authors.forename: Dingyong
authors.initials: D
authors.lastname: Wang
authors.forename: Chunxia
authors.initials: C
authors.lastname: Liu
mesh_terms: D031247:Celastrus / Q000737:chemistry*; D015394:Molecular Structure; D053978:Pentacyclic Triterpenes; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents; D014315:Triterpenes / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D053978:Pentacyclic Triterpenes; D012997:Solvents; D014315:Triterpenes; C050414:celastrol