title: Roles of macrophage stimulating protein and tyrosine kinase receptor RON in smoke-induced airway inflammation of rats.
abstract: OBJECTIVE
  To investigate the roles of macrophage stimulating protein (MSP) and its tyrosine kinase receptor RON in smoke-induced airway inflammation of rats.
  
  
  METHODS
  Inhalation of combustion smoke was administered in rats to induce airway inflammation. Alveolar macrophages (AM) of healthy and smoking rats were isolated at different time points, cultured and then treated with different concentrations of MSP for 24 h.
  
  
  RESULTS
  When compared with healthy rats, MSP increased in the serum and bronchoalveolar lavage fluid (BALF) of smoking rats in a time dependent manner. In smoking rats, the RON expression in the lung and AM was higher than in healthy rats, and these increases were time dependent. MSP stimulated the production of malondialdehyde (MDA) and reduced superoxide dismutase (SOD) activity in rat AM cells in a dose dependent manner. MSP also stimulated the release of inflammatory factors TNF-α, IL-8, IL-1β and IL-10 in rat AM in a dose-dependent manner. Moreover, at the same MSP concentration, the contents of MDA, TNF-α, IL-8 and IL-1β in the AM of smoking rates were higher than in healthy rats, while the IL-10 content and SOD activity were lower than in healthy rats.
  
  
  CONCLUSIONS
  MSP and its receptor RON are involved in the smoke-induced airway inflammation in rats via promoting AM to release inflammatory cytokines and inducing the increase of oxygen free radical.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Changyan
authors.initials: C
authors.lastname: Leng
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of North Sichuan Medical College Nanchong, Sichuan 637000, China.
authors.forename: Qinyao
authors.initials: Q
authors.lastname: Jia
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D007150:Immunohistochemistry; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D011014:Pneumonia / Q000378:metabolism / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015208:Smoke Inhalation Injury / Q000378:metabolism / Q000473:pathology*
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C082332:macrophage stimulating protein; D017228:Hepatocyte Growth Factor; C080835:RON protein; D020794:Receptor Protein-Tyrosine Kinases
keywords: Macrophage stimulating protein; alveolar macrophage; inflammatory factor