title: Fast frame scanning camera system for light-sheet microscopy.
abstract: In the interest of improving the temporal resolution for light-sheet microscopy, we designed a fast frame scanning camera system that incorporated a galvanometer scanning mirror into the imaging path of a home-built light-sheet microscope. This system transformed a temporal image sequence to a spatial one so that multiple images could be acquired during one exposure period. The improvement factor of the frame rate was dependent on the number of sub-images that could be tiled on the sensor without overlapping each other and was therefore a trade-off with the image size. As a demonstration, we achieved 960 frames/s (fps) on a CCD camera that was originally capable of recording images at only 30 fps (full frame). This allowed us to observe millisecond or sub-millisecond events with ordinary CCD cameras.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.forename: Baoli
authors.initials: B
authors.lastname: Yao
authors.forename: Runze
authors.initials: R
authors.lastname: Li
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Yang
authors.forename: Tong
authors.initials: T
authors.lastname: Peng
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Dan
authors.initials: D
authors.lastname: Dan
authors.forename: Tong
authors.initials: T
authors.lastname: Ye
mesh_terms: D000818:Animals; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007909:Lenses; D008027:Light; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D059629:Signal-To-Noise Ratio
chemical_list: D005456:Fluorescent Dyes