title: Isolation, Identification, and Sequencing of a Goose-Derived Newcastle Disease Virus and Determination of Its Pathogenicity.
abstract: In August 2010, geese in the Meihekou area of Jilin province in China were found to be infected by a pathogen that caused a disease similar to Newcastle disease. To determine the causative agent of the infections, a virus was isolated from liver tissues of infected geese, followed by a pathogenicity determination. The isolated virus was named NDV/White Goose/China/Jilin(Meihekou)/MHK-1/2010. Specific primers were designed to amplify the whole genome of the MHK-1 virus, followed by sequencing and splicing of the entire genome. Sequencing and phylogenetic analysis of MHK-1 showed that the isolate was a virulent strain of Newcastle disease virus. The MHK-1 genome is 15,192 nucleotides long, and it belongs to the class II branch of Newcastle disease viruses, as evidenced by the amino acid sequence (112R-R-Q-K-R-F117) of the F protein. The hemagglutinin titer was 1:128 to 1:512. The chicken embryo mean death time, the intracerebral pathogenicity index, and the median lethal dose of chicken embryos of MHK-1 were 43 hr, 1.63, and 10(9)/ml, respectively, which revealed that the newly isolated MHK-1 strain is strongly pathogenic to geese.
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Chen
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Zi-Bing
authors.initials: ZB
authors.lastname: Li
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Gui-Xue
authors.initials: GX
authors.lastname: Hu
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Song-Zhi
authors.initials: SZ
authors.lastname: Gu
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ying
authors.affiliation: A Department of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China.|C Department of Bioengineering, College of Life Science and Technology, Dalian University, Dalian, Liaoning 116622, China.
authors.forename: Feng-Shan
authors.initials: FS
authors.lastname: Gao
mesh_terms: D000818:Animals; D046090:Anseriformes; D002642:Chick Embryo; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D010802:Phylogeny; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D014764:Viral Proteins
keywords: Newcastle disease virus; geese; genome; isolation; pathogenicity