title: Facile synthesis of novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds for secretome study.
abstract: Novel magnetic silica nanoparticles functionalized with layer-by-layer detonation nanodiamonds (dNDs) were prepared by coating single submicron-size magnetite particles with silica and subsequently modified with dNDs. The resulting layer-by-layer dND functionalized magnetic silica microspheres (Fe3O4@SiO2@[dND]n) exhibit a well-defined magnetite-core-silica-shell structure and possess a high content of magnetite, which endow them with high dispersibility and excellent magnetic responsibility. Meanwhile, dNDs are known for their high affinity and biocompatibility towards peptides or proteins. Thus, a novel convenient, fast and efficient pretreatment approach of low-abundance peptides or proteins was successfully established with Fe3O4@SiO2@[dND]n microspheres. The signal intensity of low-abundance peptides was improved by at least two to three orders of magnitude in mass spectrometry analysis. The novel microsphere also showed good tolerance to salt. Even with a high concentration of salt, peptides or proteins could be isolated effectively from samples. Therefore, the convenient and efficient enrichment process of this novel layer-by-layer dND-functionalized microsphere makes it a promising candidate for isolation of protein in a large volume of culture supernatant for secretome analysis. In the application of Fe3O4@SiO2@[dND]n in the secretome of hepatoma cells, 1473 proteins were identified and covered a broad range of pI and molecular weight, including 377 low molecular weight proteins.
authors.affiliation: Department of Chemistry & Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China. weiliming@fudan.edu.cn pyyang@fudan.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Haojie
authors.initials: H
authors.lastname: Lu
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Zhou
authors.forename: Liming
authors.initials: L
authors.lastname: Wei
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D019295:Computational Biology; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D058612:Nanodiamonds / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D058612:Nanodiamonds; D011506:Proteins; D012822:Silicon Dioxide