title: [DISTRIBUTION OF TWO-LOCUS HAPLOTYPES OF MICROBIAL COMPONENT SENSOR GENES TLR1 AND TLR6 IN MAJOR POPULATIONS OF SOUTH URALS].
abstract: OBJECTIVE
  Evaluate the fraction of various TLR1-TLR6 haplotypes in populations of Russians Bashkir and Nagaybak of Chelyabinsk Region.
  
  
  METHODS
  Potential donors of stem cells from Chelyabinsk Region Station of Blood Transfusion registry were included into the study and split into 3 populations: Russians (81), Bashkir (78) and Nagaybak (84). Genotyping by 2 polymorphisms of TLR1 and TLR6 genes was carried out in all the 3 groups. Point polymorphism of TLR1 gene 1805T>G was determined by polymorphism analysis of length of restriction fragments, and polymorphism of TLR6 gene 745C>T by PCR using sequence-specific primers.
  
  
  RESULTS
  TLR1 1805*G-TLR6 745*T haplotype occurs in population of Russians (42%) and relatively rare--among Bashkir (17%). An inverse picture is observed for TLR1 1805*T-TLR6 745*C haplotype: a more frequent spread among Bashkir (65%) and relatively rare occurrence in Russians (23%). Frequencies of the mentioned haplotypes, that occupy intermediate position compared with corresponding parameters for populations of Russians and Bashkir, were detected for Nagaybak, that, probably, reflects complex pathways of settling of their ancestors and effects of other non-adaptation factors.
  
  
  CONCLUSIONS
  Frequencies of TLR1-TLR6 two-locus haplotypes in major populations of South Urals were determined for the first time. Further studies in this field will allow better understanding of features of immune response and sensitivity to infections in various populations.
authors.forename: A L
authors.initials: AL
authors.lastname: Burmistrova
authors.forename: A V
authors.initials: AV
authors.lastname: Evdokimov
authors.forename: D S
authors.initials: DS
authors.lastname: Stashkevich
authors.forename: Yu Yu
authors.initials: YY
authors.lastname: Filippova
authors.forename: T A
authors.initials: TA
authors.lastname: Suslova
mesh_terms: D005260:Female; D056426:Genetic Loci; D006239:Haplotypes; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012426:Russia / Q000208:ethnology; D051194:Toll-Like Receptor 1 / Q000235:genetics*; D051216:Toll-Like Receptor 6 / Q000235:genetics*
chemical_list: C495368:TLR6 protein, human; D051194:Toll-Like Receptor 1; D051216:Toll-Like Receptor 6