title: Genetic discovery for oil production and quality in sesame.
abstract: Oilseed crops are used to produce vegetable oil. Sesame (Sesamum indicum), an oilseed crop grown worldwide, has high oil content and a small diploid genome, but the genetic basis of oil production and quality is unclear. Here we sequence 705 diverse sesame varieties to construct a haplotype map of the sesame genome and de novo assemble two representative varieties to identify sequence variations. We investigate 56 agronomic traits in four environments and identify 549 associated loci. Examination of the major loci identifies 46 candidate causative genes, including genes related to oil content, fatty acid biosynthesis and yield. Several of the candidate genes for oil content encode enzymes involved in oil metabolism. Two major genes associated with lignification and black pigmentation in the seed coat are also associated with large variation in oil content. These findings may inform breeding and improvement strategies for a broad range of oilseed crops.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Xin
authors.identifier: http://orcid.org/0000-0003-2294-3256
authors.initials: X
authors.lastname: Wei
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Kunyan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Linhai
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Li
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhu
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Danlin
authors.initials: D
authors.lastname: Fan
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Luohe Academy of Agricultural Sciences, Luohe 462300, China.
authors.forename: Xianmei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Tang
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Zhou
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Chuanrang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Luohe Academy of Agricultural Sciences, Luohe 462300, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
authors.forename: Ruichun
authors.initials: R
authors.lastname: Zhong
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Tian
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Ziruo
authors.initials: Z
authors.lastname: Wen
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Weng
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: National Center for Gene Research, Collaborative Innovation Center for Genetics and Development, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture of People's Republic of China, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.
authors.forename: Xiurong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017343:Genes, Plant; D055106:Genome-Wide Association Study; D008969:Molecular Sequence Data; D012639:Seeds / Q000378:metabolism; D012715:Sesame Oil / Q000096:biosynthesis*; D031685:Sesamum / Q000235:genetics* / Q000378:metabolism
chemical_list: D012715:Sesame Oil