title: Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
abstract: To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G. The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88. Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC). Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively. The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Central Hospital of Taian, Taian, Shandong 271000, PR China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Meng
authors.affiliation: Shandong Institutes of Standardization, Ji'nan, Shandong 250000, PR China.
authors.forename: Guoyin
authors.initials: G
authors.lastname: Zhai
authors.affiliation: Academy of Agricultural Sciences, Shandong 271018, PR China.
authors.forename: Yongheng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: jia__le@126.com.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D009005:Monosaccharides / Q000737:chemistry; D020940:Reishi / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D009005:Monosaccharides
keywords: Antioxidant activity; Ganoderma lucidum polysaccharide; Spent mushroom compost