title: Degradation and interconversion of plant pteridines during sample preparation and ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: The degradation and interconversion of a selected set of pterins (dihydroneopterin, hydroxymethyldihydropterin, dihydroxanthopterin, neopterin, hydroxymethylpterin, xanthopterin, 6-formylpterin, 6-carboxypterin and pterin), spiked to charcoal-treated potato and Arabidopsis thaliana matrix was investigated, together with their relative recovery in potato and A. thaliana. As a result, a matrix-specific procedure for the ultra-high performance liquid chromatography-tandem mass spectrometry based determination of 6 aromatic pterins (neopterin, hydroxymethylpterin, xanthopterin, 6-formylpterin, 6-carboxypterin and pterin) is proposed: 1.5ml of an N2-flushed, alkaline (pH=10) extraction solvent is added to 200mg of plant sample. After boiling and homogenization, the samples are incubated: Arabidopsis samples for 30min at room temperature, while shaking, and potato samples for 2h at 37°C (applying a dienzyme treatment with α-amylase and protease). After a final boiling step, the samples are ultrafiltrated and resulting extracts are analyzed by UHPLC-MS/MS.
authors.affiliation: Laboratory of Toxicology, Department of Bioanalysis, Ghent University, Ottergemsesteenweg 460, B-9000 Gent, Belgium. Electronic address: Jeroen.VanDaele@UGent.be.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Van Daele
authors.affiliation: Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: Dieter.Blancquaert@UGent.be.
authors.forename: Dieter
authors.initials: D
authors.lastname: Blancquaert
authors.affiliation: Laboratory of Toxicology, Department of Bioanalysis, Ghent University, Ottergemsesteenweg 460, B-9000 Gent, Belgium. Electronic address: Filip.Kiekens@UGent.be.
authors.forename: Filip
authors.initials: F
authors.lastname: Kiekens
authors.affiliation: Laboratory of Functional Plant Biology, Department of Physiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: Dominique.VanDerStraeten@UGent.be.
authors.forename: Dominique
authors.initials: D
authors.lastname: Van Der Straeten
authors.affiliation: Laboratory of Toxicology, Department of Bioanalysis, Ghent University, Ottergemsesteenweg 460, B-9000 Gent, Belgium. Electronic address: Willy.Lambert@UGent.be.
authors.forename: Willy E
authors.initials: WE
authors.lastname: Lambert
authors.affiliation: Laboratory of Toxicology, Department of Bioanalysis, Ghent University, Ottergemsesteenweg 460, B-9000 Gent, Belgium. Electronic address: Christophe.Stove@UGent.be.
authors.forename: Christophe P
authors.initials: CP
authors.lastname: Stove
mesh_terms: D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D011621:Pteridines / Q000737:chemistry* / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D011621:Pteridines
keywords: (U)HPLC–MS/MS; 6-Carboxypterin (PubChem CID: 70361); 6-Formylpterin (PubChem CID: 150847); 6-Hydroxymethyl-7,8-dihydropterin (PubChem CID: 218); 6-Hydroxymethylpterin (PubChem CID: 69736); 7,8-Dihydroneopterin (PubChem CID: 65074); 7,8-Dihydroxanthopterin (PubChem CID: 135959); Arabidopsis; Degradation; Interconversion; Neopterin (PubChem CID: 444632); Potato; Pterin (PubChem CID: 73000); Pterins; Stability; Xanthopterin (PubChem CID: 8397)