title: Interaction between ORF24 and ORF34 in the Kaposi's Sarcoma-Associated Herpesvirus Late Gene Transcription Factor Complex Is Essential for Viral Late Gene Expression.
abstract: Transcription of herpesviral late genes is stimulated after the onset of viral DNA replication but otherwise restricted. Late gene expression in gammaherpesviruses requires the coordination of six early viral proteins, termed viral transactivation factors (vTFs). Here, we mapped the organization of this protein complex for Kaposi's sarcoma-associated herpesvirus. Disruption of this complex via point mutation of the interaction interface between the open reading frame 24 (ORF24) and ORF34 vTFs ablated both late gene expression and viral replication.
authors.affiliation: Division of Infectious Diseases and Immunity, School of Public Health, University of California, Berkeley, California, USA.
authors.forename: Zoe H
authors.initials: ZH
authors.lastname: Davis
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Hesser
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.
authors.forename: Jimin
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California, USA Department of Plant & Microbial Biology, University of California, Berkeley, California, USA Howard Hughes Medical Institute, University of California, Berkeley, California, USA glaunsinger@berkeley.edu.
authors.forename: Britt A
authors.initials: BA
authors.lastname: Glaunsinger
mesh_terms: D002460:Cell Line; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D055503:Protein Multimerization; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D050505:Mutant Proteins; D014764:Viral Proteins