title: Amoeboid movement in protozoan pathogens.
abstract: Entamoeba histolytica, the causative agent of amoebiasis, is a protozoan parasite characterised by its amoeboid motility, which is essential to its survival and invasion of the human host. Elucidating the molecular mechanisms leading to invasion of human tissues by E. histolytica requires a quantitative understanding of how its cytoskeleton deforms and tailors its mode of migration to the local microenvironment. Here we review the wide range of methods available to extract biophysical information from amoeboid cells, from interventional techniques to computational modelling approaches, and discuss how recent developments in bioimaging and bioimage informatics can complement our understanding of cellular morphodynamics at the intracellular level.
authors.affiliation: Institut Pasteur, Bioimage Analysis Unit, Department of Cell Biology & Infection, Paris, France; CNRS UMR 3691 "Pathological and Physiological Cell Dynamics", Paris, France. Electronic address: adufour@pasteur.fr.
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Dufour
authors.affiliation: Institut Pasteur, Bioimage Analysis Unit, Department of Cell Biology & Infection, Paris, France; CNRS UMR 3691 "Pathological and Physiological Cell Dynamics", Paris, France. Electronic address: jcolivo@pasteur.fr.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Olivo-Marin
authors.affiliation: Institut Pasteur, Cell Biology of Parasitism Unit, Department of Cell Biology & Infection, Paris, France; INSERM U786, Paris, France. Electronic address: nguillen@pasteur.fr.
authors.forename: Nancy
authors.initials: N
authors.lastname: Guillen
mesh_terms: D000562:Amebiasis / Q000469:parasitology*; D003198:Computer Simulation; D004748:Entamoeba histolytica / Q000502:physiology*; D006790:Host-Parasite Interactions; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008858:Microscopy, Phase-Contrast; D008954:Models, Biological; D009068:Movement / Q000502:physiology
keywords: Amoeboid movement; Bioimage informatics; Biophysical measurements; Entamoeba histolytica