title: [INTRA-ERYTHROCYTE INVASION OF ESCHERICHIA COLI STRAINS WITH VARIOUS LEVELS OF ANTI-HEMOGLOBIN ACTIVITY IN EXPERIMENT].
abstract: OBJECTIVE
  Study of intra-erythrocyte invasion of Escherichia coli strains with various levels of anti-hemoglobin activity on a model of generalized infection.
  
  
  METHODS
  Experiments were carried out on 72 male mice. Generalized infection was induced by intravenous administration of E. coli strain day culture suspension with various values of hemolytic and anti-hemoglobin activity. The levels of hemoglobin in animal blood was determined by hemoglobin-cyanide method. Intra-erythrocyte invasion of E.coli was studied by using laser scanning confocal microscopy (LSCM).
  
  
  RESULTS
  The largest decline (p < 0.05) of hemoglobin levels was detected in animals, infected with E. coli strains with a high levels of antiHbA and HA (58.3 ± 0.6 g/l and 69.7 ± 1.1 g/l). Using LSCM, microogranisms with a high levels of antiHbA were established to penetrate erythrocytes more frequently (70 ± 3.4%), than with a low (5.5 ± 1.7%).
  
  
  CONCLUSIONS
  The largest frequency of bacteria detection in erythrocytes and the highest decline of blood hemoglobin levels were noted during infection of animals with E. coli strains with high anti-hemoglobin activity.
authors.forename: E A
authors.initials: EA
authors.lastname: Schuplova
authors.forename: S B
authors.initials: SB
authors.lastname: Fadeev
authors.forename: O V
authors.initials: OV
authors.lastname: Bukharin
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D004926:Escherichia coli / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000473:pathology; D006454:Hemoglobins / Q000378:metabolism*; D008297:Male; D051379:Mice
chemical_list: D006454:Hemoglobins