title: Open Questions on the Origin of Eukaryotes.
abstract: Despite recent progress, the origin of the eukaryotic cell remains enigmatic. It is now known that the last eukaryotic common ancestor was complex and that endosymbiosis played a crucial role in eukaryogenesis at least via the acquisition of the alphaproteobacterial ancestor of mitochondria. However, the nature of the mitochondrial host is controversial, although the recent discovery of an archaeal lineage phylogenetically close to eukaryotes reinforces models proposing archaea-derived hosts. We argue that, in addition to improved phylogenomic analyses with more comprehensive taxon sampling to pinpoint the closest prokaryotic relatives of eukaryotes, determining plausible mechanisms and selective forces at the origin of key eukaryotic features, such as the nucleus or the bacterial-like eukaryotic membrane system, is essential to constrain existing models.
authors.affiliation: Unité d'Ecologie, Systématique et Evolution, Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud/Paris-Saclay, AgroParisTech, 91405 Orsay, France. Electronic address: puri.lopez@u-psud.fr.
authors.forename: Purificación
authors.initials: P
authors.lastname: López-García
authors.affiliation: Unité d'Ecologie, Systématique et Evolution, Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud/Paris-Saclay, AgroParisTech, 91405 Orsay, France.
authors.forename: David
authors.initials: D
authors.lastname: Moreira
mesh_terms: D001105:Archaea; D005075:Biological Evolution; D056890:Eukaryota / Q000145:classification*; D005057:Eukaryotic Cells; D008928:Mitochondria; D010802:Phylogeny; D013559:Symbiosis
keywords: archaea; eukaryogenesis; membrane; metabolism; origin of nucleus; symbiosis; syntrophy