title: Resistance Selection and Characterization of Chlorantraniliprole Resistance in Plutella xylostella (Lepidoptera: Plutellidae).
abstract: The diamondback moth, Plutella xylostella (L.), is considered one of the most damaging lepidopteran pests, and it has developed resistance to all conventional insecticide classes in the field. Chlorantraniliprole is the first commercial insecticide that belongs to the new chemical class of diamide insecticides. But, P. xylostella have already shown resistance to chlorantraniliprole in China. After 52 generations of selection with chlorantraniliprole, ∼48.17-fold resistance was observed. The resistant strain showed cross-resistance to flubendiamide (7.29-fold), abamectin (6.11-fold), and cyantraniliprole (3.31-fold). Quantitative real-time polymerase chain reaction analysis showed that the expression of the ryanodine receptor gene was higher in the resistant strain than that in the susceptible strain. Enzyme assays indicated that cytochrome P450 activity in the resistant strain was 4.26 times higher compared with the susceptible strain, whereas no difference was seen for glutathione-S-transferase and esterase. Moreover, the toxicity of chlorantraniliprole in the resistant strain could be synergized by piperonyl butoxide, but not by diethyl maleate, and S,S,S-tributyl phosphorothioate. These results can serve as an important base for guiding the use of insecticide in field and delaying the development of pests that are resistant to the insecticides.
authors.affiliation: Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Plant Protection, Cotton Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, People's Republic of China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Yu-Bo
authors.initials: YB
authors.lastname: Ning
authors.affiliation: Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China.
authors.forename: Kang
authors.initials: K
authors.lastname: Qiao
authors.affiliation: Department of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, People's Republic of China. kywang@sdau.edu.cn.
authors.forename: Kai-Yun
authors.initials: KY
authors.lastname: Wang
mesh_terms: D000818:Animals; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole
keywords: Plutella xylostella; chlorantraniliprole; resistance mechanism; ryanodine receptor