title: Antioxidant and anti-ageing activities of citrus-based juice mixture.
abstract: The production of excessive reactive oxygen species by exposure to oxidative stress and solar radiation are primary factors in skin damage. We examined the effects of a citrus-based juice mixture and its bioactive compounds on antioxidant and anti-ageing activities in human dermal fibroblasts and hairless mice via the regulation of antioxidant enzymes and the mitogen-activated protein kinase pathway. The citrus-based juice mixture reduced H2O2-induced cell damage and intracellular reactive oxygen species production in human dermal fibroblasts. Citrus-based juice mixture pretreatment suppressed the activation of the H2O2-mediated mitogen-activated protein kinase pathway by activating the expression of activator protein 1 and matrix metalloproteinases. Moreover, it increased the expression levels of antioxidant enzymes such as glutathione reductase, catalase and manganese superoxide dismutase. In addition, oral administration of the citrus-based juice mixture decreased skin thickness and wrinkle formation and increased collagen content on an ultraviolet light B-exposed hairless mouse. These results indicate that the citrus-based juice mixture is a potentially healthy beverage for the prevention of oxidative stress-induced premature skin ageing.
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Dan-Bi
authors.initials: DB
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Gi-Hae
authors.initials: GH
authors.lastname: Shin
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Young-Hyun
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jin-Ha
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jong Seok
authors.initials: JS
authors.lastname: Lee
authors.affiliation: EBO Co., LTD., Cheongju 361-748, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Song
authors.affiliation: Department of Biology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choe
authors.affiliation: Hurum Central Research Institute, Hurum Co., Ltd., Ochang 363-880, Republic of Korea.
authors.forename: In-Jae
authors.initials: IJ
authors.lastname: Park
authors.affiliation: Hurum Central Research Institute, Hurum Co., Ltd., Ochang 363-880, Republic of Korea. Electronic address: dusvnd608@hurum.co.kr.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Cho
authors.affiliation: Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: loh99@kangwon.ac.kr.
authors.forename: Ok-Hawn
authors.initials: OH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000975:Antioxidants; D002957:Citrus / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D015595:Skin Aging
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide
keywords: Anti-ageing; Antioxidant; Citrus-based juice mixture; Hairless mice; Human dermal fibroblasts