title: A spectrometric method for hydrogen peroxide concentration measurement with a reusable and cost-efficient sensor.
abstract: In this study we developed a low cost sensor for measuring the concentration of hydrogen peroxide (H₂O₂) in liquids utilizing a spectrometric method. The sensor was tested using various concentrations of a peroxidase enzyme immobilized on a glass substrate. H₂O₂ can be catalyzed by peroxidase and converted into water and oxygen. The reagent 4-amino-phenazone takes up oxygen together with phenol to form a colored product that has absorption peaks at 510 nm and 450 nm. The transmission intensity is strongly related to the hydrogen peroxide concentration, so can be used for quantitative analysis. The measurement range for hydrogen peroxide is from 5 × 10(-)⁵% to 1 × 10(-3)% (0.5 ppm to 10 ppm) and the results show high linearity. This device can achieve a sensitivity and resolution of 41,400 (photon count/%) and 3.49 × 10(-5)% (0.35 ppm), respectively. The response time of the sensor is less than 3 min and the sensor can be reused for 10 applications with similar performance.
authors.affiliation: Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan. cchsu@saturn.yzu.edu.tw.
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan. s1010756@mail.yzu.edu.tw.
authors.forename: Yuan-Rong
authors.initials: YR
authors.lastname: Lo
authors.affiliation: Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan. s1020759@mail.yzu.edu.tw.
authors.forename: Yu-Chian
authors.initials: YC
authors.lastname: Lin
authors.affiliation: Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan. s1020729@mail.yzu.edu.tw.
authors.forename: Yi-Cen
authors.initials: YC
authors.lastname: Shi
authors.affiliation: Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan. s1010716@mail.yzu.edu.tw.
authors.forename: Pang-Lung
authors.initials: PL
authors.lastname: Li
mesh_terms: D002138:Calibration; D017743:Equipment Reuse; D006861:Hydrogen Peroxide / Q000032:analysis*; D057230:Limit of Detection; D055095:Optics and Photonics / Q000295:instrumentation* / Q000379:methods*; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D006861:Hydrogen Peroxide
keywords: hydrogen peroxide; optical sensor; spectrometry