title: Methyloterrigena soli gen. nov., sp. nov., a methanol-utilizing bacterium isolated from chloroethylene-contaminated soil.
abstract: A Gram-stain-negative, aerobic, short rod-shaped and motile bacterial strain, designated M48T, was isolated from a soil sample contaminated with chloroethylene compounds. Strain M48T was able to biodegrade methanol as a sole carbon source. Based on 16S rRNA gene sequences, strain M48T was closely related to Devosia chinhatensis IPL18T, Devosia insulae DS-56T, Vasilyevaea enhydra 9bT and Devosia psychrophila Cr7-05T, showing sequence similarities of 96.0, 95.8, 95.6 and 95.5 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M48T formed a distinct lineage within the family Hyphomicrobiaceae of the class Alphaproteobacteria. Strain M48T contains Q-10 as the predominant ubiquinone, and C18 : 1ω7c 11-metyl, C16 : 0, C19 : 0 cyclo ω8c and C18 : 1ω7c as the major fatty acids. The polar lipid profile includes diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and dimannosyldiacylglycerol. The DNA G+C content of strain M48T was 60.3 mol%. Based on phenotypic, phylogenetic and chemotaxonomic data, strain M48T represents a novel species of a new genus within the family Hyphomicrobiaceae, for which the name Methyloterrigena soli gen. nov., sp. nov. is proposed. The type strain of the type species is M48T ( = KEMB 224-262T = JCM 30821T).
authors.affiliation: Department of Bioengineering, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
authors.forename: Hyun-Sook
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon 443-760, Republic of Korea.
authors.forename: Sang-Seob
authors.initials: SS
authors.lastname: Lee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041921:Hyphomicrobiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry; D014752:Vinyl Chloride
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014451:Ubiquinone; C024989:coenzyme Q10; D014752:Vinyl Chloride; D000432:Methanol