title: The role of human papillomavirus in head and neck squamous cell carcinoma: A case control study on a southern Chinese population.
abstract: HPV plays a role in the development of a portion of head and neck squamous cell carcinoma (HNSCC), but only limited information on its role in southern Chinese population is available. A multicenter case-control study was conducted. HPV type, viral integration, E6/7 mRNA expression status, and TP53 mutation were determined. A total of 228 HNSCC were recruited including 137 (60.1%) oral SCC, 34 (14.9%) oropharyngeal SCC, 31 (13.6%) laryngeal SCC, 21 (9.2%) hypopharyngeal SCC, and 5 (2.2%) lip and paranasal sinus SCC. High-risk HPV infection was found in 7.5% (17/228) of HNSCC, but only a small proportion of samples had evidence of viral integration (5.3%, 12/228) or E6/7 mRNA expression (4.4%, 10/228). HPV infection with oncogenic phenotype (integration and E6/7 mRNA expression) was significantly more common in oropharyngeal SCC than controls (9/34, 26.5% vs. 0/42, 0.0%, P < 0.001). Smoking showed a significant association with HNSCC, oropharyngeal SCC, and laryngeal SCC. TP53 mutation was associated with HNSCC (P < 0.001). Older age, TP53 mutation, and HPV16 infection with oncogenic phenotypes were independently associated factors for HNSCC with odds ratios of 1.03 (1.02-1.05), 3.38 (1.71-6.66), and 9.19 (1.13-74.68), respectively. High-risk HPV infection of head and neck mucosa is not uncommon in the Hong Kong population. This study found that 26-30% of oropharyngeal carcinoma was associated with HPV infection, mostly HPV16, and that smoking which predisposes to TP53 mutations was another important risk factor.
authors.affiliation: School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Josette S Y
authors.initials: JS
authors.lastname: Chor
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Alexander C
authors.initials: AC
authors.lastname: Vlantis
authors.affiliation: Department of Surgery, United Christian Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Tam-Lin
authors.initials: TL
authors.lastname: Chow
authors.affiliation: Department of Dentistry, United Christian Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Siu-Chung
authors.initials: SC
authors.lastname: Fung
authors.affiliation: Department of Surgery, Kwong Wah Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Fiona Y H
authors.initials: FY
authors.lastname: Ng
authors.affiliation: Department of Surgery, Queen Elizabeth Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Chi-Hung
authors.initials: CH
authors.lastname: Lau
authors.affiliation: Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Amy B W
authors.initials: AB
authors.lastname: Chan
authors.affiliation: Department of Pathology, Queen Elizabeth Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Luen-Cheung
authors.initials: LC
authors.lastname: Ho
authors.affiliation: Department of Surgery, North District Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Wing-Hang
authors.initials: WH
authors.lastname: Kwong
authors.affiliation: Department of Pathology, United Christian Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Mandy N S
authors.initials: MN
authors.lastname: Fung
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.|Department of Otorhinolaryngology, Head and Neck Surgery, Yan Chai Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Eddy W H
authors.initials: EW
authors.lastname: Lam
authors.affiliation: Department of Surgery, Princess Margaret Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Kwong-Lin
authors.initials: KL
authors.lastname: Mak
authors.affiliation: School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Holly C Y
authors.initials: HC
authors.lastname: Lam
authors.affiliation: Department of Surgery, United Christian Hospital, Hong Kong Special Administrative Region, China.
authors.forename: Amy S Y
authors.initials: AS
authors.lastname: Kok
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Wendy C S
authors.initials: WC
authors.lastname: Ho
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Apple C M
authors.initials: AC
authors.lastname: Yeung
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Chan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D006258:Head and Neck Neoplasms / Q000453:epidemiology / Q000821:virology*; D006723:Hong Kong / Q000453:epidemiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D012307:Risk Factors; D012907:Smoking; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D016662:Virus Integration; D055815:Young Adult
chemical_list: D009856:Oncogene Proteins, Viral; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: China; HPV; Hong Kong; head and neck cancer; oral cavity cancer; oropharyngeal cancer