title: Colorimetric and Fluorometric Assays for Dopamine with a Wide Concentration Range Based on Fe-MIL-88NH2 Metal-organic Framework.
abstract: Metal-organic framework Fe-MIL-88NH2 as a dual colorimetric and fluorometric sensor has been designed for a wide range of dopamine quantitative detection. It is easy to implement the assay for visual detection of dopamine based on restraining the color change of the 3,3',5,5'-tetramethylbenzidine-H2O2 system that is catalyzed by Fe-MIL-88NH2 with intrinsic peroxidase-like catalytic activity. The linear range is from 50 nM to 30 μM. In addition, the Fe-MIL-88NH2 can exhibit a dramatic decrease of its fluorescent intensity when exposed to dopamine, which may be attributed to the electron transfer from the Fe-MIL-88NH2 to the oxidized dopamine-quinone. The linear response range is from 30 μM to 4 mM. Meanwhile, both colorimetric and fluorometric methods exhibit higher selectivity for DA over a number of possible interfering substances. Furthermore, the proposed method has been successfully applied to detect DA in human serum samples, which suggests its great potential for analytical applications.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Li
mesh_terms: D001560:Benzidines / Q000737:chemistry; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D004298:Dopamine / Q000032:analysis* / Q000097:blood; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D009942:Organometallic Compounds / Q000737:chemistry*
chemical_list: D001560:Benzidines; D009942:Organometallic Compounds; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D007501:Iron; D004298:Dopamine