title: First-Pass Angiography in Mice Using FDG-PET: A Simple Method of Deriving the Cardiovascular Transit Time Without the Need of Region-of-Interest Drawing.
abstract: In this study, we developed a simple and robust semi-automatic method to measure the right ventricle to left ventricle (RV-to-LV) transit time (TT) in mice using 2-[18F]fluoro-2-deoxy-D-glucose (FDG) positron emission tomography (PET). The accuracy of the method was first evaluated using a 4-D digital dynamic mouse phantom. The RV-to-LV TTs of twenty-nine mouse studies were measured using the new method and compared to those obtained from the conventional ROI-drawing method. The results showed that the new method correctly separated different structures (e.g., RV, lung, and LV) in the PET images and generated corresponding time activity curve (TAC) of each structure. The RV-to-LV TTs obtained from the new method and ROI method were not statistically different (P = 0.20; r = 0.76). We expect that this fast and robust method is applicable to the pathophysiology of cardiovascular diseases using small animal models such as rats and mice.
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA, ( cwu@mednet.ucla.edu ).
authors.forename: Hsiao-Ming
authors.initials: HM
authors.lastname: Wu
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Kreissl
authors.affiliation: UCLA-DOE Institute for Molecular Medicine, Los Angeles, CA 90095 USA and also with the Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Heinrich R
authors.initials: HR
authors.lastname: Schelbert
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Ladno
authors.affiliation: Department of Neurosurgery, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Prins
authors.affiliation: Department of Biomathematics, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Kooresh
authors.initials: K
authors.lastname: Shoghi-Jadid
authors.affiliation: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Arion
authors.initials: A
authors.lastname: Chatziioannou
authors.affiliation: UCLA-DOE Institute for Molecular Medicine, Los Angeles, CA 90095 USA and also with the Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Phelps
authors.affiliation: UCLA-DOE Institute for Molecular Medicine, Los Angeles, CA 90095 USA and also with the Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095 USA.
authors.forename: Sung-Cheng
authors.initials: SC
authors.lastname: Huang
keywords: Cardiovascular transit time; first-pass angiography; mice; positron emission tomography