title: Efficacy of triptolide on the apoptosis of tonsillar mononuclear cells from patients with IgA nephropathy.
abstract: OBJECTIVE
  This study aimed to evaluate the extent of apoptosis of tonsillar mononuclear cells (TMCs) derived from patients with IgA nephropathy (IgAN) and the effects of triptolide (TP) on the apoptosis of these TMCs.
  
  
  METHODS
  TMCs were isolated from tonsillar tissues of patients with IgAN or chronic tonsillitis (control group). Rates of TMCs apoptosis were measured by annexin V-fluorescein isocyanate (FITC)/propidium iodide (PI)-labeled flow cytometry (FCM). Expression levels of Bcl-2 family proteins were quantified by immunohistochemistry of fixed tonsillar sections and Western blot analyzes of TMCs lysates. TMCs from IgAN patients were treated 10, 20, or 30 ng/mL TP for 24 h and then evaluated for viability by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, for the percentage of apoptotic cells by FCM, and for the relative expression levels of Bcl-2 family proteins by Western blot analysis.
  
  
  RESULTS
  Compared to TMCs from the control group, TMCs from the IgAN group demonstrated lower rates of apoptosis, higher expression levels of the anti-apoptosis proteins Bcl-2 and Bcl-xL, and lower expression levels of the pro-apoptosis protein Bax. Treatment of IgAN patient-derived TMCs with 10, 20, or 30 ng/mL TP for 24 h suppressed the viability and promoted the apoptosis of TMCs in a dose-dependent manner. Western blot analysis revealed a TP dose-dependent decrease in Bcl-2 and Bcl-xL expression levels, in parallel with increased Bax protein levels.
  
  
  CONCLUSIONS
  TMCs from IgAN patients may be in a state of inhibited apoptosis mediated by Bcl-2 family proteins, which may be reversed by TP treatment.
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Yan
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Youming
authors.initials: Y
authors.lastname: Peng
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Chan
authors.initials: C
authors.lastname: Liu
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Muyao
authors.initials: M
authors.lastname: Ye
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Peng
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Liyu
authors.initials: L
authors.lastname: He
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Wang
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: a Department of Nephrology , The Second Xiangya Hospital, Central South University, Hunan Key Lab of Kidney Disease and Blood Purification , People's Republic of China and.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
authors.affiliation: b Department of Hematology/Institute of Molecular Hematology , the Second XiangYa Hospital, Central South University , People's Republic of China.
authors.forename: Guangsen
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D004852:Epoxy Compounds / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005922:Glomerulonephritis, IGA / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014066:Palatine Tonsil / Q000276:immunology* / Q000378:metabolism; D010616:Phenanthrenes / Q000494:pharmacology / Q000627:therapeutic use*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D055815:Young Adult
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; D007166:Immunosuppressive Agents; D010616:Phenanthrenes; D019253:Proto-Oncogene Proteins c-bcl-2; C001899:triptolide
keywords: Apoptosis; Bcl-2; IgA nephropathy; tonsil; triptolide