title: Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.
abstract: We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. The limit of detection of this LFA was 0.13 ng/mL IgE, ∼100 times lower than those of previously reported IgE assays.
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.
authors.forename: Meena
authors.initials: M
authors.lastname: Adhikari
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Strych
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Jinsu
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.
authors.forename: Heather
authors.initials: H
authors.lastname: Goux
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.
authors.forename: Sagar
authors.initials: S
authors.lastname: Dhamane
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.
authors.forename: Mohan-Vivekanandan
authors.initials: MV
authors.lastname: Poongavanam
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Anna E V
authors.initials: AE
authors.lastname: Hagström
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Katerina
authors.initials: K
authors.lastname: Kourentzi
authors.affiliation: Department of Chemical & Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Jacinta C
authors.initials: JC
authors.lastname: Conrad
authors.affiliation: Department of Biology & Biochemistry, University of Houston , Houston, Texas 77004, United States.|Department of Chemical & Biomolecular Engineering, University of Houston , Houston, Texas 77204, United States.|Centro de Biotecnología FEMSA, Tecnológico de Monterrey , Monterrey, Nuevo León, Mexico.
authors.forename: Richard C
authors.initials: RC
authors.lastname: Willson
mesh_terms: D052158:Aptamers, Peptide / Q000032:analysis* / Q000737:chemistry*; D017104:Bacteriophage M13 / Q000737:chemistry*; D001681:Biological Assay; D007073:Immunoglobulin E / Q000032:analysis*; D046915:Penicillin-Binding Proteins / Q000032:analysis*; D013211:Staphylococcus aureus / Q000737:chemistry
chemical_list: D052158:Aptamers, Peptide; D046915:Penicillin-Binding Proteins; D007073:Immunoglobulin E