title: Evaluating Nuclear Factor NF-κB Activation following Bone Trauma: A Pilot Study in a Wistar Rats Model.
abstract: The present study investigated the moment of peak NF-kB activation and its dissipation in the cortical bone in the femur of Wistar rat stimulated by surgical trauma. Sixty-five Wistar rats were divided into 13 groups (n = 5 per group): eight experimental groups (expG 1-8) divided based on the euthanasia time point (zero, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h and 24 h) and five sham control groups (conG 1-5) killed at zero, 1 h, 2 h, 4 h and 6 h, respectively. A 1.8-mm-diameter defect was generated 0.5 mm from the femur proximal joint using a round bur to induce the surgical trauma. Overall, the activation peak of NF-κB in the cortical bone was 6 h (expG5 group) independent of the evaluated position; this peak was significantly different compared to those in the other groups (p < 0.05). The surgical trauma resulted in a spread of immune markings throughout the cortical bone with an accentuation in the knee region. The present study provides the first evidence that the NF-κB activation peak was established after 6 hours in the cortical bone of Wistar rats. The signs from a surgical trauma can span the entire cortical bone and are not limited to the damaged region.
authors.affiliation: Anatomy Department, Biomedical Science Institute, Universidade de São Paulo, São Paulo, Brazil; Implantology Department, São Leopoldo Mandic, Campinas, Brasil.
authors.forename: Marcos Barbosa
authors.initials: MB
authors.lastname: Salles
authors.affiliation: Biotecnos Research Center, Santa Maria, Rio Grande do Sul, Brazil; Catholic University of Uruguay, Montevideo, Uruguay; Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos, SP, Brazil.
authors.forename: Sergio Alexandre
authors.initials: SA
authors.lastname: Gehrke
authors.affiliation: Department of Periodontology and Oral Implantology, Dental Research Division, University of Guarulhos, Guarulhos, SP, Brazil.
authors.forename: Jamil Awad
authors.initials: JA
authors.lastname: Shibli
authors.affiliation: Anatomy Department, Biomedical Science Institute, Universidade de São Paulo, São Paulo, Brazil; Orthopedy Department, Ernst Moritz Arndt University, Greifswald, Germany.
authors.forename: Sergio
authors.initials: S
authors.lastname: Allegrini
authors.affiliation: Anatomy Department, Biomedical Science Institute, Universidade de São Paulo, São Paulo, Brazil; Implantology Department, São Leopoldo Mandic, Campinas, Brasil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Yoshimoto
authors.affiliation: Anatomy Department, Biomedical Science Institute, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: König
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D005269:Femur / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000473:pathology; D007718:Knee Injuries / Q000235:genetics* / Q000473:pathology; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014947:Wounds and Injuries / Q000235:genetics* / Q000473:pathology
chemical_list: D016328:NF-kappa B