title: Gelatin blending and sonication of chitosan nanofiber mats produce synergistic effects on hemostatic functions.
abstract: To improve the hemostatic function of chitosan nanofiber mats, we studied the synergetic effects of gelatin blending and porosity control. Gelatin-blended-chitosan (Chi-Gel) nanofiber mats were evaluated with respect to surface morphology, mechanical properties and wettability, and functionally tested in a blood clotting study. The blood clotting efficiency of Chi-Gel nanofiber mats using rabbit whole blood in vitro was superior to that of chitosan nanofibers. Moreover, Chi-Gel nanofiber mats with enlarged porosity, produced by ultra-sonication, showed improved blood clotting efficiency, cell viability and cell infiltration compared with non-sonicated Chi-Gel nanofiber mats. Field-emission scanning electron microscopy revealed a richer density of platelets on sonicated nanofiber mats than on non-sonicated nanofiber mats after 3 min of blood clotting. The proliferation of human dermal fibroblast cells on sonicated Chi-Gel nanofiber mats using the DNA assay was higher than that on non-sonicated chitosan nanofiber mats after 7 days of culture. Confocal z-stack images showed that sonicated Chi-Gel nanofiber mats with high porosity supported active cell migration and infiltration into the 3-dimensional nanofiber mats. These results suggest that hydrophilic gelatin blending and sonication of chitosan nanofiber mats yields synergistic effects that not only improve hemostatic function but also promote wound repair.
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul 139-240, Republic of Korea.
authors.forename: Bon Kang
authors.initials: BK
authors.lastname: Gu
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul 139-240, Republic of Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul 139-240, Republic of Korea.
authors.forename: Min Sup
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul 139-240, Republic of Korea.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Food and Microbial Technology, Seoul Women's University, Seoul 139-774, Republic of Korea.
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul 139-240, Republic of Korea. Electronic address: chkim@kcch.re.kr.
authors.forename: Chun-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D004357:Drug Synergism; D005347:Fibroblasts; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology*; D006454:Hemoglobins / Q000737:chemistry; D006490:Hemostatics / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D013010:Sonication
chemical_list: D001672:Biocompatible Materials; D006454:Hemoglobins; D006490:Hemostatics; D005780:Gelatin; D048271:Chitosan
keywords: Gelatin-blended-chitosan nanofiber mats; Hemostatics; Sonication