title: Characterization of inner membrane protein YciB in Escherichia coli: YciB interacts with cell elongation and division proteins.
abstract: The function of inner membrane protein YciB in Escherichia coli has not been identified. In this study, the membrane topology of the protein that contains five transmembrane domains was clarified. YciB was found to interact with various proteins involved in cell elongation and cell division using a bacterial two-hybrid system. It was also found that the deletion mutant of yciB is susceptible to the low osmolarity. These observations together with previous reports indicate that YciB is involved in synthesis of the cell envelope by interacting with cell elongation and cell division complexes.
authors.affiliation: Central laboratory, Maternity and Child Healthcare Hospital, Ailong Road, 518172, Shenzhen, China.|Department of Biology, Faculty of Science, Kobe University, Rokko, Kobe, Hyogo 657-8501, Japan.
authors.forename: Gaochi
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Biology, Faculty of Science, Kobe University, Rokko, Kobe, Hyogo 657-8501, Japan.
authors.forename: Noor Afiza
authors.initials: NA
authors.lastname: Badaluddin
authors.affiliation: Department of Biology, Faculty of Science, Kobe University, Rokko, Kobe, Hyogo 657-8501, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Kitakawa
mesh_terms: D002455:Cell Division / Q000502:physiology*; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D054730:Protein Interaction Domains and Motifs / Q000235:genetics*; D017384:Sequence Deletion / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C043426:inner membrane protein, E coli
keywords: Escherichia coli; YciB; membrane topology; two-hybrid analysis