title: NMR-based metabolic profiling in healthy individuals overfed different types of fat: links to changes in liver fat accumulation and lean tissue mass.
abstract: BACKGROUND
  Overeating different dietary fatty acids influence the amount of liver fat stored during weight gain, however, the mechanisms responsible are unclear. We aimed to identify non-lipid metabolites that may differentiate between saturated (SFA) and polyunsaturated fatty acid (PUFA) overfeeding using a non-targeted metabolomic approach. We also investigated the possible relationships between plasma metabolites and body fat accumulation.
  
  
  METHODS
  In a randomized study (LIPOGAIN study), n=39 healthy individuals were overfed with muffins containing SFA or PUFA. Plasma samples were precipitated with cold acetonitrile and analyzed by nuclear magnetic resonance (NMR) spectroscopy. Pattern recognition techniques were used to overview the data, identify variables contributing to group classification and to correlate metabolites with fat accumulation.
  
  
  RESULTS
  We previously reported that SFA causes a greater accumulation of liver fat, visceral fat and total body fat, whereas lean tissue levels increases less compared with PUFA, despite comparable weight gain. In this study, lactate and acetate were identified as important contributors to group classification between SFA and PUFA (P<0.05). Furthermore, the fat depots (total body fat, visceral adipose tissue and liver fat) and lean tissue correlated (P(corr)>0.5) all with two or more metabolites (for example, branched amino acids, alanine, acetate and lactate). The metabolite composition differed in a manner that may indicate higher insulin sensitivity after a diet with PUFA compared with SFA, but this needs to be confirmed in future studies.
  
  
  CONCLUSIONS
  A non-lipid metabolic profiling approach only identified a few metabolites that differentiated between SFA and PUFA overfeeding. Whether these metabolite changes are involved in depot-specific fat storage and increased lean tissue mass during overeating needs further investigation.
authors.affiliation: Department Medicinal Chemistry; Analytical Pharmaceutical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Elmsjö
authors.affiliation: Department of Public Health and Caring Sciences; Clinical Nutrition and Metabolism, Uppsala University, Uppsala, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Rosqvist
authors.affiliation: Department Medicinal Chemistry; Analytical Pharmaceutical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: M K R
authors.initials: MK
authors.lastname: Engskog
authors.affiliation: Department Medicinal Chemistry; Analytical Pharmaceutical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Haglöf
authors.affiliation: Department of Radiology, Oncology and Radiation Sciences; Radiology, Uppsala University, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Kullberg
authors.affiliation: Department of Public Health and Caring Sciences; Clinical Nutrition and Metabolism, Uppsala University, Uppsala, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Iggman
authors.affiliation: Department of Radiology, Oncology and Radiation Sciences; Radiology, Uppsala University, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Johansson
authors.affiliation: Department of Radiology, Oncology and Radiation Sciences; Radiology, Uppsala University, Uppsala, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Ahlström
authors.affiliation: Department Medicinal Chemistry; Analytical Pharmaceutical Chemistry, Uppsala University, Uppsala, Sweden.|Medical Product Agency, Uppsala, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Arvidsson
authors.affiliation: Department of Public Health and Caring Sciences; Clinical Nutrition and Metabolism, Uppsala University, Uppsala, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Risérus
authors.affiliation: Department Medicinal Chemistry; Analytical Pharmaceutical Chemistry, Uppsala University, Uppsala, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Pettersson