title: Is Peroxiredoxin II's peroxidase activity strongly inhibited in human erythrocytes?
abstract: H2O2 elimination in human erythrocytes is mainly carried out by catalase (Cat), glutathione peroxidase (GPx1) and the more recently discovered peroxiredoxin 2 (Prx2). However, the contribution of Prx2 to H2O2 consumption is still unclear. Prx2's high reactivity with H2O2 (kPrx2=10×10(7) M(-1)s(-1), kCat =7×10(7) M(-1)s(-1), kGPx1 =4×10(7) M(-1)s(-1)) and high abundance ([Prx2]= 570µM, [Cat]= 32µM, [GPx1]= 1µM) suggest that under low H2O2 supply rates it should consume >99% of the H2O2. However, extensive evidence indicates that in intact erythrocytes Prx2 contributes no more than Cat to H2O2 consumption. In order for this to be attained, Prx2's effective rate constant with H2O2would have to be just ~10(5) M(-1)s(-1), much lower than that determined in multiple experiments with the purified proteins. Nevertheless, nearly all Prx2 is oxidized within 1min of exposing erythrocytes to a H2O2 bolus, which is inconsistent with an irreversible inhibition. A mathematical model of the H2O2 metabolism in human erythrocytes [Benfeitas et al. (2014) Free Radic. Biol. Med.] where Prx2 either has a low kPrx2 or is subject to a strong (>99%) but readily reversible inhibition achieves quantitative agreement with detailed experimental observations of the responses of the redox status of Prx2 in human erythrocytes and suggests functional advantages of this design (see companion abstract). By contrast, a variant where Prx2 is fully active with kPrx2=10(8) M(-1)s(-1) shows important qualitative discrepancies. Altogether, these results suggest that Prx2's peroxidase activity is strongly inhibited in human erythrocytes. We acknowledge fellowship SFRH/BD/51199/2010, grants PEst-C/SAU/LA0001/2013-2014, PEst-OE/QUI/UI0612/2013, PEst-OE/QUI/UI0313/2014, and FCOMP-01-0124-FEDER-020978 (PTDC/QUI-BIQ/119657/2010) co-financed by FEDER through the COMPETE program and by FCT.
authors.affiliation: CNC (Center for Neuroscience and Cell Biology), University of Coimbra, Portugal. Electronic address: rui.benfeitas@gmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Benfeitas
authors.affiliation: CNC (Center for Neuroscience and Cell Biology), University of Coimbra, Portugal.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Selvaggio
authors.affiliation: DQB (Centro de Química e Bioquímica), Departamento de Química e Bioquímica, Portugal.
authors.forename: Fernando
authors.initials: F
authors.lastname: Antunes
authors.affiliation: CNC (Center for Neuroscience and Cell Biology), University of Coimbra, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Coelho
authors.affiliation: CNC (Center for Neuroscience and Cell Biology), University of Coimbra, Portugal.
authors.forename: Armindo
authors.initials: A
authors.lastname: Salvador