title: A model microfluidics-based system for the human and mouse retina.
abstract: The application of microfluidics technologies to the study of retinal function and response holds great promise for development of new and improved treatments for patients with degenerative retinal diseases. Restoration of vision via retinal transplantation therapy has been severely limited by the low numbers of motile cells observed post transplantation. Using modern soft lithographic techniques, we have developed the μRetina, a novel and convenient biomimetic microfluidics device capable of examing the migratory behavior of retinal lineage cells within biomimetic geometries of the human and mouse retina. Coupled computer simulations and experimental validations were used to characterize and confirm the formation of chemical concentration gradients within the μRetina, while real-time images within the device captured radial and theta cell migration in response to concentration gradients of stromal derived factor (SDF-1), a known chemoattractant. Our data underscore how the μRetina can be used to examine the concentration-dependent migration of retinal progenitors in order to enhance current therapies, as well as develop novel migration-targeted treatments.
authors.affiliation: Department of Biomedical Engineering, City College of New York, New York, NY, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Mishra
authors.affiliation: Department of Biomedical Engineering, City College of New York, New York, NY, USA.
authors.forename: Ankush
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Department of Biology, Lehman College, New York, NY, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Redenti
authors.affiliation: Department of Biomedical Engineering, City College of New York, New York, NY, USA. vazquez@ccny.cuny.edu.
authors.forename: Maribel
authors.initials: M
authors.lastname: Vazquez
mesh_terms: D000818:Animals; D032701:Biomimetics / Q000295:instrumentation; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000737:chemistry; D019295:Computational Biology; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation*; D008954:Models, Biological; D015203:Reproducibility of Results; D012160:Retina / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D054377:Chemokine CXCL12
keywords: Diffusion; Migration; Progenitor; Retina; SDF-1