title: Phenolic compounds: Strong inhibitors derived from lignocellulosic hydrolysate for 2,3-butanediol production by Enterobacter aerogenes.
abstract: Lignocellulosic biomass are attractive feedstocks for 2,3-butanediol production due to their abundant supply and low price. During the hydrolysis of lignocellulosic biomass, various byproducts are formed and their effects on 2,3-butanediol production were not sufficiently studied compared to ethanol production. Therefore, the effects of compounds derived from lignocellulosic biomass (weak acids, furan derivatives and phenolics) on the cell growth, the 2,3-butanediol production and the enzymes activity involved in 2,3-butanediol production were evaluated using Enterobacter aerogenes ATCC 29007. The phenolic compounds showed the most toxic effects on cell growth, 2,3-butanediol production and enzyme activity, followed by furan derivatives and weak acids. The significant effects were not observed in the presence of acetic acid and formic acid. Also, feasibility of 2,3-butanediol production from lignocellulosic biomass was evaluated using Miscanthus as a feedstock. In the fermentation of Miscanthus hydrolysate, 11.00 g/L of 2,3-butanediol was obtained from 34.62 g/L of reducing sugar. However, 2,3-butanediol was not produced when the concentration of total phenolic compounds in the hydrolysate increased to more than 1.5 g/L. The present study provides useful information to develop strategies for biological production of 2,3-butanediol and to establish biorefinery for biochemicals from lignocellulosic biomass.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Ju Hun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Sung Bong
authors.initials: SB
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Ja Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea.
authors.forename: Hah Young
authors.initials: HY
authors.lastname: Yoo
authors.affiliation: Department of Chemical Engineering, Kwangwoon University, Seoul, Republic of Korea. chpark@kw.ac.kr.
authors.forename: Chulhwan
authors.initials: C
authors.lastname: Park
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea. kimsw@korea.ac.kr.
authors.forename: Seung Wook
authors.initials: SW
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D002072:Butylene Glycols / Q000378:metabolism*; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D005663:Furans / Q000633:toxicity; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D056804:Biofuels; D002072:Butylene Glycols; D005663:Furans; D010636:Phenols; C036909:lignocellulose; C026978:2,3-butylene glycol; D008031:Lignin
keywords: 2,3-butanediol; Enterobacter aerogenes; Inhibitory effects; Lignocellulosic hydrolysate; Miscanthus