title: Integration of a miniature quartz crystal microbalance with a microfluidic chip for amyloid beta-Aβ42 quantitation.
abstract: A miniature quartz crystal microbalance (mQCM) was integrated with a polydimethylsiloxane (PDMS) microfluidic device for on-chip determination of amyloid polypeptide-Aβ42. The integration techniques included photolithography and plasma coupling. Aβ42 antibody was immobilized on the mQCM surface using a cross-linker method, and the resonance frequency of mQCM shifted negatively due to antibody-antigen binding. A linear range from 0.1 µM to 3.2 µM was achieved. By using matrix elimination buffer, i.e., matrix phosphate buffer containing 500 µg/mL dextran and 0.5% Tween 20, Aβ42 could be successfully detected in the presence of 75% human serum. Additionally, high temperature treatments at 150 °C provided a valid method to recover mQCM, and PDMS-mQCM microfluidic device could be reused to some extent. Since the detectable Aβ42 concentration could be as low as 0.1 µM, which is close to cut-off value for Alzheimer patients, the PDMS-mQCM device could be applied in early Alzheimer's disease diagnosis.
authors.affiliation: Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. taowenyan2002@gmail.com.|College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China. taowenyan2002@gmail.com.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Tao
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. xieqj@hunnu.edu.cn.
authors.forename: Qingji
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. wang8641@hotmail.com.
authors.forename: Hairui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. smke@szu.edu.cn.
authors.forename: Shanming
authors.initials: S
authors.lastname: Ke
authors.affiliation: Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. lin.peng@szu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: Shenzhen Key Laboratory of Special Functional Materials & Shenzhen Engineering Laboratory for Advance Technology of Ceramics, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. zengxier@szu.edu.cn.|College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060, China. zengxier@szu.edu.cn.
authors.forename: Xierong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D016229:Amyloid beta-Peptides / Q000097:blood*; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010446:Peptide Fragments / Q000097:blood*; D058525:Quartz Crystal Microbalance Techniques / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D016229:Amyloid beta-Peptides; D004129:Dimethylpolysiloxanes; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); C013830:baysilon
keywords: amyloid polypeptide–Aβ42; integration; miniature quartz crystal microbalance; polydimethylsiloxane