title: Immunoproteomic Profiling of Antiviral Antibodies in New-Onset Type 1 Diabetes Using Protein Arrays.
abstract: The rapid rise in the incidence of type 1 diabetes (T1D) suggests the involvement of environmental factors including viral infections. We evaluated the association between viral infections and T1D by profiling antiviral antibodies using a high-throughput immunoproteomics approach in patients with new-onset T1D. We constructed a viral protein array comprising the complete proteomes of seven viruses associated with T1D and open reading frames from other common viruses. Antibody responses to 646 viral antigens were assessed in 42 patients with T1D and 42 age- and sex-matched healthy control subjects (mean age 12.7 years, 50% males). Prevalence of antiviral antibodies agreed with known infection rates for the corresponding virus based on epidemiological studies. Antibody responses to Epstein-Barr virus (EBV) were significantly higher in case than control subjects (odds ratio 6.6; 95% CI 2.0-25.7), whereas the other viruses showed no differences. The EBV and T1D association was significant in both sex and age subgroups (≤12 and >12 years), and there was a trend toward early EBV infections among the case subjects. These results suggest a potential role for EBV in T1D development. We believe our innovative immunoproteomics platform is useful for understanding the role of viral infections in T1D and other disorders where associations between viral infection and disease are unclear.
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Bian
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Garrick
authors.initials: G
authors.lastname: Wallstrom
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Amy
authors.initials: A
authors.lastname: Davis
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Jin
authors.initials: J
authors.lastname: Park
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Andrea
authors.initials: A
authors.lastname: Throop
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Jason
authors.initials: J
authors.lastname: Steel
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL.
authors.forename: Clive
authors.initials: C
authors.lastname: Wasserfall
authors.affiliation: Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL.
authors.forename: Desmond
authors.initials: D
authors.lastname: Schatz
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL.
authors.forename: Mark
authors.initials: M
authors.lastname: Atkinson
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ Joshua.LaBaer@asu.edu ji.qiu@asu.edu.
authors.forename: Ji
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ Joshua.LaBaer@asu.edu ji.qiu@asu.edu.
authors.forename: Joshua
authors.initials: J
authors.lastname: LaBaer
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology; D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003384:Coxsackievirus Infections / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology* / Q000821:virology; D020077:Endogenous Retroviruses / Q000276:immunology; D029822:Enterovirus B, Human / Q000276:immunology; D020031:Epstein-Barr Virus Infections; D005260:Female; D005968:Glutamate Decarboxylase / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D057166:High-Throughput Screening Assays; D006801:Humans; D008297:Male; D009107:Mumps / Q000276:immunology; D009109:Mumps virus / Q000276:immunology; D040081:Protein Array Analysis; D040901:Proteomics; D012401:Rotavirus / Q000276:immunology; D012400:Rotavirus Infections / Q000276:immunology; D012409:Rubella / Q000276:immunology; D012412:Rubella virus / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D055815:Young Adult; D000075703:Zinc Transporter 8
chemical_list: D000914:Antibodies, Viral; D001323:Autoantibodies; D027682:Cation Transport Proteins; C096322:ICA512 autoantibody; C492522:SLC30A8 protein, human; D014764:Viral Proteins; D000075703:Zinc Transporter 8; D005968:Glutamate Decarboxylase