title: HPIP promotes thyroid cancer cell growth, migration and EMT through activating PI3K/AKT signaling pathway.
abstract: Hematopoietic pre-B cell leukemia transcription factor (PBX)-interacting protein (HPIP), a co-repressor for the transcription factor PBX, is a nucleo-cytoplasmic shuttling protein. Increasing evidence suggests that HPIP is an oncogene which is frequently overexpressed in many human carcinomas. However, the role of HPIP in thyroid carcinoma is still unclear. Therefore, in this study, we investigated the role of HPIP in thyroid carcinoma, and explored the underling mechanism. We found that the expression of HPIP is upregulated in thyroid carcinoma cell lines. Knockdown of HPIP inhibits thyroid carcinoma cell proliferation, migration/invasion and epithelial-mesenchymal transition (EMT). HPIP knockdown also reduces thyroid tumor growth in nude mice. Furthermore, knockdown of HPIP significantly inhibits the expression of phosphorylated PI3K and AKT in thyroid carcinoma cells. Taken together, these results suggest that knockdown of HPIP inhibits the proliferation, migration and EMT by suppressing the PI3K/AKT pathway, and HPIP may be a potential therapeutic target for the treatment of thyroid carcinoma.
authors.affiliation: Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China. Electronic address: wang_shunchang@126.com.
authors.forename: Shun-chang
authors.initials: SC
authors.lastname: Wang
authors.affiliation: Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
authors.forename: Da-sen
authors.initials: DS
authors.lastname: Chai
authors.affiliation: Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
authors.forename: Chuan-bo
authors.initials: CB
authors.lastname: Chen
authors.affiliation: Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
authors.forename: Zheng-ye
authors.initials: ZY
authors.lastname: Wang
authors.affiliation: Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D000818:Animals; D002277:Carcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008819:Mice, Nude; D010641:Phenotype; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden
chemical_list: C469750:HCFC1R1 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt
keywords: Epithelial–mesenchymal transition (EMT); Hematopoietic pre-B cell leukemia transcription factor (PBX)-interacting protein (HPIP); Migration; Proliferation; Thyroid carcinoma