title: Humanized Mouse Models to Study Cell-Mediated Immune Responses to Liver-Stage Malaria Vaccines.
abstract: Malaria vaccine development is hampered by the lack of small animal models that recapitulate human immune responses to Plasmodium falciparum. We review the burgeoning literature on humanized mice for P. falciparum infection, including challenges in engraftment of human immune cells, hepatocytes, and erythrocytes. Recent advances in immune-compromised mouse models and stem cell technology have already enabled proof of concept that the entire parasite life cycle can be sustained in a murine model and that adaptive human immune responses to several parasite stages can be measured. Nonetheless, optimization is needed to achieve a reproducible and relevant murine model for malaria vaccine development. This review is focused on the complexities of T cell development in a mouse humanized with both a lymphoid system and hepatocytes. An understanding of this will facilitate the use of humanized mice in the development of liver-stage vaccines.
authors.affiliation: Institute for Glycomics, Griffith University, Queensland, Australia; Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada. Electronic address: michael.good@griffith.edu.au.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Good
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada; Department of Pediatrics, Division of Infectious Diseases, University of Alberta, Edmonton, Canada; School of Public Health, University of Alberta, Edmonton, Canada.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Hawkes
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada; School of Public Health, University of Alberta, Edmonton, Canada; Provincial Laboratory for Public Health, Edmonton, Canada.
authors.forename: Stephanie K
authors.initials: SK
authors.lastname: Yanow
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007111:Immunity, Cellular; D008099:Liver / Q000469:parasitology; D017780:Malaria Vaccines; D016778:Malaria, Falciparum / Q000276:immunology*; D051379:Mice; D010963:Plasmodium falciparum / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D017780:Malaria Vaccines