title: Time-lapse contact microscopy of cell cultures based on non-coherent illumination.
abstract: Video microscopy offers outstanding capabilities to investigate the dynamics of biological and pathological mechanisms in optimal culture conditions. Contact imaging is one of the simplest imaging architectures to digitally record images of cells due to the absence of any objective between the sample and the image sensor. However, in the framework of in-line holography, other optical components, e.g., an optical filter or a pinhole, are placed underneath the light source in order to illuminate the cells with a coherent or quasi-coherent incident light. In this study, we demonstrate that contact imaging with an incident light of both limited temporal and spatial coherences can be achieved with sufficiently high quality for most applications in cell biology, including monitoring of cell sedimentation, rolling, adhesion, spreading, proliferation, motility, death and detachment. Patterns of cells were recorded at various distances between 0 and 1000 μm from the pixel array of the image sensors. Cells in suspension, just deposited or at mitosis focalise light into photonic nanojets which can be visualised by contact imaging. Light refraction by cells significantly varies during the adhesion process, the cell cycle and among the cell population in connection with every modification in the tridimensional morphology of a cell.
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Gabriel
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Dorothée
authors.initials: D
authors.lastname: Balle
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bigault
authors.affiliation: CEA, Léti-DOPT, F-38000 Grenoble, France.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Pornin
authors.affiliation: CEA, Léti-DOPT, F-38000 Grenoble, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Gétin
authors.affiliation: CEA, Léti-DTBS, F-38000 Grenoble, France.
authors.forename: François
authors.initials: F
authors.lastname: Perraut
authors.affiliation: IAB, CRI INSERM/UJF U823, 38706 La Tronche, France.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Block
authors.affiliation: CEA, IBS, F-38000 Grenoble, France.
authors.forename: François
authors.initials: F
authors.lastname: Chatelain
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Picollet-D'hahan
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Gidrol
authors.affiliation: CEA, iRTSV-BGE, F-38000 Grenoble, France.|INSERM, BGE, F-38000 Grenoble, France.|Université Grenoble Alpes, iRTSV-BGE, F-38000 Grenoble, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Haguet
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D018715:Microscopy, Video / Q000379:methods*; D059008:Time-Lapse Imaging / Q000379:methods*