title: [A Large Number of Circulating Tumor Cells(CTCs)Can Be Isolated from Samples Obtained by Using Leukapheresis Procedures].
abstract: We hypothesized that a large number of circulating tumor cells(CTCs)may be isolated from samples obtained by using the leukapheresis procedures that are utilized to collect peripheral blood mononuclear cells for dendritic cell vaccine therapy. We utilized the CellSearch System to determine the number of CTCs in samples obtained by using leukapheresis in 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In all patients, a large number of CTCs were isolated. The mean number of CTCs per tumor was 17.1(range 10-34)in colorectal cancer, 10.0(range 2-27)in breast cancer, and 24.0(range 2-42)in gastric cancer. We succeeded in culturing the isolated CTCs from 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In conclusion, compared to conventional methods, a large number of CTCs can be obtained by using leukapheresis procedures. The molecular analyses of the CTCs isolated by using this method should be promising in the development of personalized cancer treatments.
authors.affiliation: Dept. of Gastrointestinal Pediatric Surgery, Tokyo Medical University.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Soya
authors.forename: Jyunichi
authors.initials: J
authors.lastname: Taguchi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nagakawa
authors.forename: Osamu
authors.initials: O
authors.lastname: Takahashi
authors.forename: Norimasa
authors.initials: N
authors.lastname: Sandoh
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kasuya
authors.forename: Naoki
authors.initials: N
authors.lastname: Umeda
authors.forename: Masato
authors.initials: M
authors.lastname: Okamoto
authors.forename: Shunichi
authors.initials: S
authors.lastname: Tsujitani
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tsuchida
mesh_terms: D000368:Aged; D001943:Breast Neoplasms / Q000473:pathology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000473:pathology; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D013274:Stomach Neoplasms / Q000473:pathology