title: MicroRNA-590 is an EMT-suppressive microRNA involved in the TGFβ signaling pathway.
abstract: Over the last few decades, the epithelial-to-mesenchymal transition (EMT) has been identified as being involved in a number of aspects of physiological processes and various pathological events, including embryonic development and renal fibrosis. Transforming growth factor‑β receptor 2 (TGFβR2) is a widely studied gene, which fulfils a vital role in the TGFβ signaling pathway and exerts a crucial function in the progression of EMT. Previous studies demonstrated that the dysregulation of microRNAs (miRNAs) is considered to be associated with the EMT process. However, the precise functional involvement of miRNAs in EMT remains to be fully elucidated. In the present study, the level of miR‑590 was decreased in an EMT model in vitro and in vivo. Furthermore, the overexpression of miR‑590 inhibited EMT by upregulating the epithelial marker, E‑cadherin, and downregulating the mesenchymal markers, laminin, α‑smooth muscle actin (α‑SMA) and collagen, in the human kidney 2 (HK2) cell line. Furthermore, TGFβR2 was negatively regulated by miR‑590. In addition, performing a knockdown of TGFβR2 with small‑interfering RNA had an effect similar to miR‑590 on EMT in the HK2 cell line, whereas the transfection of pCMV‑tag2B‑TGFβR2 reversed the effect of miR‑590 on EMT in HK2 cells. Taken together, the present study demonstrated that miR-590 is a novel EMT-suppressive microRNA, which targets TGFβR2.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Tianming
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Xianggui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Ni
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics Designated by Chinese Ministry of Education, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, P.R. China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Weng
mesh_terms: D000818:Animals; D002460:Cell Line; D058750:Epithelial-Mesenchymal Transition; D057809:HEK293 Cells; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C568221:MIRN590 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II