title: Electrochemical & osteoblast adhesion study of engineered TiO2 nanotubular surfaces on titanium alloys.
abstract: TiO2 nanotubes were grafted on the surface of cpTi, Ti6Al4V and Ti6Al4V-ELI with the aim to provide a new podium for human pre-osteoblast cell (MC3T3) adhesion and proliferation. The surface morphology and chemistry of these alloys were examined with scanning electron microscopy and energy dispersive x-ray spectroscopy. TiO2 nanotubes were further characterized by cyclic potentiodynamic polarization tests and electrochemical impedance spectroscopy. The vertically aligned nanotubes were subjected to pre-osteoblast cell proliferation in order to better understand cell-material interaction. The study demonstrated that these cells interact differently with nanotubes of different titanium alloys. The significant acceleration in the growth rate of pre-osteoblast cell adhesion and proliferation is also witnessed. Additionally, the cytotoxicity of the leached metal ions was evaluated by using a tetrazolium-based bio-assay, MTS. Each group of data was operated for p<0.05, concluded one way ANOVA to investigate the significance difference.
authors.affiliation: School of Engineering and Technology, Central Michigan University, Mt. Pleasant, MI, USA.
authors.forename: Zia Ur
authors.initials: ZU
authors.lastname: Rahman
authors.affiliation: School of Engineering and Technology, Central Michigan University, Mt. Pleasant, MI, USA. Electronic address: haide1w@cmich.edu.
authors.forename: Waseem
authors.initials: W
authors.lastname: Haider
authors.affiliation: Department of Mechanical Engineering, University of Texas-Pan American, Edinburg, TX, USA.
authors.forename: Luis
authors.initials: L
authors.lastname: Pompa
authors.affiliation: Department of Metallurgy & Materials Engineering, CEET, University of the Punjab, 54590 Lahore, Pakistan; Department of Materials Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
authors.forename: K M
authors.initials: KM
authors.lastname: Deen
mesh_terms: D000497:Alloys; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D055664:Electrochemical Techniques; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; D014025:Titanium
keywords: Electrochemical; Impedance spectroscopy; Nanotubes; Osteoblast; Potentiodynamic