title: Magnetic bead-quantum dot assay for detection of a biomarker for traumatic brain injury.
abstract: Current diagnostic methods for traumatic brain injury (TBI), which accounts for 15% of all emergency room visits, are limited to neuroimaging modalities. The challenges of accurate diagnosis and monitoring of TBI have created the need for a simple and sensitive blood test to detect brain-specific biomarkers. Here we report on an assay for detection of S100B, a putative biomarker for TBI, using antibody-conjugated magnetic beads for capture of the protein, and antibody-conjugated quantum dots for optical detection. From Western Blot, we show efficient antigen capture and concentration by the magnetic beads. Using magnetic bead capture and quantum dot detection in serum samples, we show a wide detection range and detection limit below the clinical cut-off level.
authors.affiliation: Department of Materials Science and Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA. searson@jhu.edu.
authors.forename: Chloe
authors.initials: C
authors.lastname: Kim
authors.forename: Peter C
authors.initials: PC
authors.lastname: Searson
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000097:blood*; D001930:Brain Injuries / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008280:Magnetics; D045663:Quantum Dots / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D001426:Bacterial Proteins; D015415:Biomarkers; C034284:IgG Fc-binding protein, Streptococcus; D011994:Recombinant Proteins; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human