title: Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit.
abstract: In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
authors.affiliation: Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Yan-li
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Protected Horticulture, Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Zhi-he
authors.initials: ZH
authors.lastname: Wang
mesh_terms: D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism; D005638:Fruit / Q000502:physiology*; D018551:Solanum lycopersicum / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*; D014018:Tissue Distribution; D043324:beta-Fructofuranosidase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D013395:Sucrose; D043324:beta-Fructofuranosidase
keywords: Fruit development; Invertase inhibitor; Overexpression; Solanum lycopersicum; Transient transformation system