title: Activity of dental pulp cells in semisolid 3D cultures initiated by transforming growth factor-β1 and bone morphogenetic protein 2, 4.
abstract: The intention of this study was to investigate the effect of modified 3D culture conditions on dental pulp cells (DPCs). DPCs were isolated from extracted primary molar, premolar, and wisdom teeth. Tooth samples were divided into three groups as control group; plated into methyl cellulose medium without any supplementation, growth factor (GF) group; supplemented with bone morphogenetic proteins (BMP2, BMP4), transforming growth factor&mdash;&#946;1 (TGF&mdash;&#946;1) and growth factor+conditioned medium (GF+CM) group; supplemented with both growth factors and pulp conditioned medium. The DPCs were tested for colony forming ability, proliferation capacity and morphology. The highest colony forming ability was detected in the GF and GF+CM groups of DPCs isolated from wisdom teeth. The proliferation capacity was higher in GF+CM group of DPCs isolated from primary molars, and in GF and GF+CM groups of DPCs isolated from wisdom teeth. Scanning electron microscope (SEM) observation of the wisdom teeth samples showed cell&mdash;cell interactions in the GF and GF+CM groups. Our results indicate that growth factors and pulp conditioned medium in methyl cellulose culture created proper environment to follow the behavior of dental cells three&mdash;dimensionally.
authors.affiliation: Harran University Department of Biology, Science Art Faculty Sanliurfa Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Suzergoz
authors.affiliation: Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Sepet
authors.affiliation: Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
authors.forename: A P
authors.initials: AP
authors.lastname: Erdem
authors.affiliation: Istanbul University Department of Immunology, Institute of Experimental Medicine Istanbul Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Cinar
authors.affiliation: Istanbul University Department of Pedodontics, Dentistry Faculty Istanbul Turkey.
authors.forename: G
authors.initials: G
authors.lastname: Ikikarakayali
authors.affiliation: Istanbul University Department of Oral Surgery, Dentistry Faculty Istanbul Turkey.
authors.forename: M A
authors.initials: MA
authors.lastname: Erdem
authors.affiliation: Istanbul University Department of Immunology, Institute of Experimental Medicine Istanbul Turkey ogurol&commat;istanbul.edu.tr.
authors.forename: A O
authors.initials: AO
authors.lastname: Gurol
mesh_terms: D000293:Adolescent; D000328:Adult; D001641:Bicuspid / Q000166:cytology*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002450:Cell Communication / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003114:Colony-Forming Units Assay; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third / Q000166:cytology*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D055815:Young Adult
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D053773:Transforming Growth Factor beta1