title: TransgeneOmics--A transgenic platform for protein localization based function exploration.
abstract: The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Hasse
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Germany.
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Germany.
authors.forename: Mihail
authors.initials: M
authors.lastname: Sarov
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D023281:Genomics / Q000379:methods*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D019076:Transgenes
chemical_list: D018076:DNA, Complementary; D005456:Fluorescent Dyes
keywords: BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering