title: Cooccurrence of Multiple AmpC β-Lactamases in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis in Tunisia.
abstract: Over a period of 40 months, plasmid-mediated AmpC β-lactamases were detected in Tunis, Tunisia, in 78 isolates (0.59%) of Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. In 67 isolates, only one ampC gene was detected, i.e., blaCMY-2-type (n = 33), blaACC (n = 23), blaDHA (n = 6) or blaEBC (n = 5). Multiple ampC genes were detected in 11 isolates, with the following distribution: blaMOX-2, blaFOX-3, and blaCMY-4/16 (n = 6), blaFOX-3 and blaMOX-2 (n = 3), and blaCMY-4 and blaMOX-2 (n = 2). A great variety of plasmids carrying these genes was found, independently of the species and the bla gene. If the genetic context of blaCMY-2-type is variable, that of blaMOX-2, reported in part previously, is unique and that of blaFOX-3 is unique and new.
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Tunis, Tunisie.
authors.forename: Thouraya
authors.initials: T
authors.lastname: Chérif
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Tunis, Tunisie CHU Charles Nicolle, Service de Microbiologie, Tunis, Tunisia.
authors.forename: Mabrouka
authors.initials: M
authors.lastname: Saidani
authors.affiliation: Sorbonne Université, UPMC Université Paris 06 CR7, Paris, France INSERM U1135, CIMI, Team E13, Paris, France AP-HP, Groupe Hospitalier des Hôpitaux Universitaires de l'Est Parisien, Département de Bactériologie, Paris, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Decré
authors.affiliation: Université de Tunis El Manar, Faculté de Médecine de Tunis, LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Tunis, Tunisie CHU Charles Nicolle, Service de Microbiologie, Tunis, Tunisia.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Boutiba-Ben Boubaker
authors.affiliation: Sorbonne Université, UPMC Université Paris 06 CR7, Paris, France INSERM U1135, CIMI, Team E13, Paris, France AP-HP, Groupe Hospitalier des Hôpitaux Universitaires de l'Est Parisien, Département de Bactériologie, Paris, France guillaume.arlet@tnn.aphp.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010406:Penicillins / Q000494:pharmacology; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D014416:Tunisia / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010406:Penicillins; D037521:Virulence Factors; C048795:AmpC beta-lactamases; D001618:beta-Lactamases