title: Development of liposomes entrapped in alginate beads for the treatment of colorectal cancer.
abstract: BACKGROUND
  Folic Acid conjugated liposomes encapsulating Oxaliplatin (L-OHP) were entrapped in alginate beads and further coated with Eudragit-S-100 for effective delivery to colon tumors.
  
  
  METHODS
  Liposomes were prepared by cast film method and folic acid was coupled on the surface of liposomes. They were further entrapped in alginate beads which were Eudragit coated for degradation in the colonic region. The prepared beads were characterized for shape and surface morphology, percentage entrapment efficiency and drug release studies. The in vitro drug release was investigated using a USP dissolution paddle type apparatus in different simulated gastrointestinal fluids. In vivo studies of the beads containing free drug, folic acid coupled and uncoupled liposomes bearing L-OHP was administered orally at the dose of 10mg L-OHP/kg body weight to tumor bearing NUDE/SCID mice.
  
  
  RESULTS
  γ-Scintigraphic study showed that Eudragit coated alginate beads entered into the colon of Balb/c mice between 4.20 and 4.50h after oral administration. In vivo data showed that folic acid coupled liposomes entrapped in alginate beads delivered 2.82 ± 0.58 and 21.52 ± 2.76 μg L-OHP/g tissues in the colon and tumor after 12h, reflecting its targeting potential to colon and tumor.
  
  
  CONCLUSIONS
  The results clearly demonstrate that Eudragit coated calcium alginate beads bearing folic acid coupled liposome can be used as a prospective carrier for drug delivery to colon specific tumor.
authors.affiliation: Pharmaceutics Research Laboratory, Shri Ram Institute of Technology, Faculty of Pharmacy, Jabalpur, MP, India. Electronic address: drdivyabansal1126@gmail.com.
authors.forename: Divya
authors.initials: D
authors.lastname: Bansal
authors.affiliation: Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya, Sagar, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Gulbake
authors.affiliation: Department of Medicine, Bundelkhand Medical College, Sagar, MP, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Tiwari
authors.affiliation: Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya, Sagar, India.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Jain
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible; D015179:Colorectal Neoplasms / Q000188:drug therapy; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding; D016503:Drug Delivery Systems; D065546:Drug Liberation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D051379:Mice; D008863:Microspheres; D009944:Organoplatinum Compounds / Q000008:administration & dosage; D000077150:Oxaliplatin; D010316:Particle Size; D011109:Polymethacrylic Acids; D014018:Tissue Distribution
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D006603:Hexuronic Acids; D008081:Liposomes; D009944:Organoplatinum Compounds; D011109:Polymethacrylic Acids; D000077150:Oxaliplatin; C038300:methylmethacrylate-methacrylic acid copolymer; D020723:Glucuronic Acid
keywords: Alginate beads; Colon cancer; Folic acid; Liposomes; Oxaliplatin