title: Separation of turkey lactate dehydrogenase isoenzymes using isoelectric focusing technique.
abstract: Native polyacrylamide gel electrophoresis at pH 8.8 did not allow to separate lactate dehydrogenase (LDH) isoenzymes of turkey origin. Five electrophoretically distinguishable forms of the enzyme were detected in serum and tissues of turkey using IEF technique in a pH range of 3-9. Generally, three different groups were seen: (i) those having an anodic domination (heart, kidney, pancreas, and erythrocytes) with mainly LDH-1 fraction, (ii) those having a cathodic domination (breast muscle and serum) with prevalence of LDH-5, and (iii) those with a more uniform distribution (liver, spleen, lung, and brain). The specific enzyme activity was the highest in the breast muscle, followed by heart muscle, and brain. Low activities were detected in serum, kidney, and liver.
authors.affiliation: Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy, Košice, Slovakia.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Heinová
authors.affiliation: Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy, Košice, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Kostecká
authors.affiliation: Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Košice, Slovakia.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Csank
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000302:isolation & purification; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D000080864:Lactate Dehydrogenase 5; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D014422:Turkeys / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; C409667:lactate dehydrogenase 1
keywords: Birds; Isoelectric focusing; Lactate dehydrogenase isoenzymes; Mammals; Turkey