title: Spatial light modulator phase mask implementation of wavefront encoded 3D computational-optical microscopy.
abstract: Spatial light modulator (SLM) implementation of wavefront encoding enables various types of engineered point-spread functions (PSFs), including the generalized-cubic and squared-cubic phase mask wavefront encoded (WFE) PSFs, shown to reduce the impact of sample-induced spherical aberration in fluorescence microscopy. This investigation validates dynamic experimental parameter variation of these WFE-PSFs. We find that particular design parameter bounds exist, within which the divergence of computed and experimental WFE-PSFs is of the same order of magnitude as that of computed and experimental conventional PSFs, such that model-based approaches for solving the inverse imaging problem can be applied to a wide range of SLM-WFE systems. Interferometric measurements were obtained to evaluate the SLM implementation of the numeric mask. Agreement between experiment and theory in terms of a wrapped phase, 0-2π, validates the phase mask implementation and allows characterization of the SLM response. These measurements substantiate experimental practice of computational-optical microscope imaging with an SLM-engineered PSF.
authors.forename: Sharon V
authors.initials: SV
authors.lastname: King
authors.forename: Ana
authors.initials: A
authors.lastname: Doblas
authors.forename: Nurmohammed
authors.initials: N
authors.lastname: Patwary
authors.forename: Genaro
authors.initials: G
authors.lastname: Saavedra
authors.forename: Manuel
authors.initials: M
authors.lastname: Martínez-Corral
authors.forename: Chrysanthe
authors.initials: C
authors.lastname: Preza