title: The Skewness of Alpha Beta T Cell Receptors in Peripheral Blood of the Patients with Type 1 Diabetes.
abstract: OBJECTIVE
  To detect the skewness of TCR Vα and TCR Vβ of patients with type 1 diabetes (T1D).
  
  
  METHODS
  The heparinized venous blood was collected from ten patients with T1D. The peripheral blood lymphocytes (PBL) were isolated and used to extract mRNA. Reverse amplyfication was performed for cDNA synthesis. The skewness of TCR Vα and Vβ was detected with real-time florescence quantitative polymerase chain reaction (RQ-PCR) and analyzed by DNA melting-curve analysis technique, respectively.
  
  
  RESULTS
  Among the TCR Vα genes, the skewness frequency rate (SFR) of Vα22 was 30%; both of Vα5 and Vα24 were 20%; Vα28 was the only restricted-clone gene with the SFR of 10%. In all the Vβ genes, Vβ7 and Vβ17 were the the highest expression genes, and their SFRs were both 60%. Vβ11 was near them with the SFR of 40%; the restricted clonal genes were Vβ18 and Vβ20, their SFRs were 10% and 20%, respectivley.
  
  
  CONCLUSIONS
  There are skewd genes in TCR Vα and TCR Vβ, which are probably relative to the onset of T1D.
authors.affiliation: Medical Laboratory, Affilated Hospital of Jining Medical University, Jining, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Nursing Department, Affilated Hospital of Jining Medical University, Jining, China.
authors.forename: C
authors.initials: C
authors.lastname: Kong
authors.affiliation: Medical Laboratory, Affilated Hospital of Jining Medical University, Jining, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Department of Pharmacology, The First People's Hospital of Jining City, Jining, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Medical Laboratory, Affilated Hospital of Jining Medical University, Jining, China.
authors.forename: C
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Stomatology, Affiliated Hospital of Jining Medical University, Jining, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D054504:Precursor Cells, T-Lymphoid / Q000276:immunology / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood / Q000235:genetics / Q000276:immunology
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta