title: [Expression of FBXW7 in esophageal squamous cell carcinoma and its clinical significance].
abstract: OBJECTIVE
  To evaluate the expression of FBXW7 in esophageal squamous cell carcinoma (ESCC) and to explore the correlation of FBXW7 expression with clinicopathologic features and prognosis of ESCC.
  
  
  METHODS
  Ninety cases of ESCC and twenty cases of tumor-adjacent normal tissues and forty intraepithelial neoplasia tissues were detected by immunohistochemistry methods. The expression of FBXW7 in 40 surgical ESCC tissues and tumor-adjacent normal tissues was detected by Western blotting and RT-PCR. The relationship between the expression of FBXW7, clinicopathological characteristics and prognosis was analyzed.
  
  
  RESULTS
  The positive rate of FBXW7 protein was significantly lower in the ESCC and high-grade intraepithelial neoplasia tissues than in the tumor-adjacent normal tissues and low grade intraepithelial neoplasia tissues (40.0% and 33.3% vs. 85.0% and 77.3%, χ² = 21.923, P < 0.001). The FBXW7 protein was down-regulated in ESCC tissues (P < 0.05), whereas the FBXW7 mRNA was down-regulated in ESCC tissues compared with that in the paracancerous tissues. The expression of FBXW7 was significantly correlated with TNM stage, degree of differentiation, invasion depth and lymph node metastasis (χ² =9.643, 14.908, 16.294, 10.222, respectively, P = 0.002, 0.001, 0.000, 0.001). In the ninety patients, the 5-year survival rates of cases with positive and negative expression of FBXW7 protein was 67.6% and 39.3%, respectively (χ² =6.699, P = 0.01).
  
  
  CONCLUSIONS
  The results of this study demonstrate that FBXW7 expression is significantly declined in ESCC and high grade intraepithelial neoplasia tissues, and is closely correlated with poor prognosis of this disease. FBXW7 as a tumor suppressor gene may play an important role in the carcinogenesis, development and metastasis of ESCC. These results suggest that FBXW7 may become a valuable marker for the severity and prognosis in ESCC.
authors.affiliation: Department of Gastroenterology, Tongling People's Hospital, Tongling 244000, China; Email: china_yuhai@126.com.
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Tingsheng
authors.initials: T
authors.lastname: Ling
authors.forename: Ruihua
authors.initials: R
authors.lastname: Shi
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Shu
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Zhuli
authors.initials: Z
authors.lastname: Tan
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics*; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D000077277:Esophageal Squamous Cell Carcinoma; D044783:F-Box Proteins / Q000235:genetics*; D000073979:F-Box-WD Repeat-Containing Protein 7; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger; D015996:Survival Rate; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; D012333:RNA, Messenger; D044767:Ubiquitin-Protein Ligases