title: Effect of the co-culture of human bone marrow mesenchymal stromal cells with human umbilical vein endothelial cells in vitro.
abstract: Mesenchymal stem cells (MSCs) give origin to the marrow tromal environment that supports hematopoiesis. These cells present a wide range of differentiation potentials and a complex relationship with hematopoietic stem cells (HSCs) and endothelial cells. In addition to bone marrow (BM), MSCs can be obtained from other sites in the adult or the fetus. Recent studies have shown that cocultured endothelial cells and osteoblasts are mutually promotive in bone tissues repair. In this study, we observed the effects of coculture of endothelial cells and osteoblasts at different ratios on vasculogenesis and bone formation, and we found that angiogenic effect is more effective when endothelial cells are cocultured with osteoblasts at the ratio of 4:1, and osteogenic effect is more effective at the ratio of 1:4. It is concluded that the co-culture of human bone marrow mesenchymal stromal cells with human umbilical vein endothelial cells could be a promising culture system for bone tissue engineering applications.
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Qiwang
authors.initials: Q
authors.lastname: Lin
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Linhui
authors.initials: L
authors.lastname: Wang
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Bai
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Menglin
authors.initials: M
authors.lastname: Hu
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Jianling
authors.initials: J
authors.lastname: Mo
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Haixin
authors.initials: H
authors.lastname: He
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.|b The Third Clinical School, Guangzhou Medical University , People's Republic of China.
authors.forename: Aiju
authors.initials: A
authors.lastname: Lou
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.
authors.forename: Hongpu
authors.initials: H
authors.lastname: Zhao
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: a Institute of Orthopedics and Traumatology, the Third Affiliated Hospital, Guangzhou Medical University , People's Republic of China , and.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D049109:Cell Proliferation; D018920:Coculture Techniques / Q000379:methods*; D004396:Coloring Agents / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D004396:Coloring Agents; D042461:Vascular Endothelial Growth Factor A; D000469:Alkaline Phosphatase
keywords: Endothelial cells; MSCs; osteoblasts