title: Identification of a soluble leptin receptor in crucian carp with different binding affinity to leptin-a and leptin-b.
abstract: Soluble leptin receptor (sLepR) is the main leptin-binding protein in plasma and contributes to activation of circulating leptin. In this study, we identified a sLepR in plasma of crucian carp (Carassius carassius) using a pull-down assay, and the interaction of sLepR with its ligand is confirmed by a cross-linking study. In addition, we found that leptin-a has higher affinity than leptin-b for sLepR. According to our knowledge, this is the first experimental report about the main ligand of sLepR in teleost.
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Xie
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
authors.forename: Saifan
authors.initials: S
authors.lastname: Huang
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.
authors.forename: Xiaopin
authors.initials: X
authors.lastname: Guo
authors.affiliation: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China. Electronic address: sky109@zjnu.edu.cn.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D002347:Carps / Q000097:blood / Q000378:metabolism*; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D011485:Protein Binding; D054411:Receptors, Leptin / Q000737:chemistry* / Q000378:metabolism*; D012995:Solubility; D013379:Substrate Specificity
chemical_list: D029941:Fish Proteins; D020738:Leptin; D054411:Receptors, Leptin
keywords: Crucian carp; Leptin-a; Leptin-b; Soluble leptin receptor; Teleost