title: Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients.
abstract: BACKGROUND
  Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples.
  
  
  METHODS
  In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.
  
  
  RESULTS
  Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18).
  
  
  CONCLUSIONS
  Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.
authors.affiliation: Oral Health Sciences, University of Washington, Box 357475, 1959 NE Pacific St., Seattle, WA, 98195, USA. jhtdang@gmail.com.
authors.forename: Juliet
authors.initials: J
authors.lastname: Dang
authors.affiliation: University of Washington, 1959 NE Pacific St., Seattle, WA, 98195, USA. qf@uw.edu.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Seattle Cancer Care Alliance, 825 Eastlake Ave E, Seattle, WA, 98109, USA. kdeaton@uw.edu.
authors.forename: Keith D
authors.initials: KD
authors.lastname: Eaton
authors.affiliation: University of Washington, 1959 NE Pacific St., Seattle, WA, 98195, USA. hjang324@gmail.com.
authors.forename: Hona
authors.initials: H
authors.lastname: Jang
authors.affiliation: Pathology, University of Washington, Seattle, WA, USA. nbk@uw.edu.|Harborview Medical Center, 908 Jefferson, Seattle, WA, 98104, USA. nbk@uw.edu.
authors.forename: Nancy B
authors.initials: NB
authors.lastname: Kiviat
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D009067:Mouthwashes; D009959:Oropharyngeal Neoplasms / Q000821:virology; D030361:Papillomavirus Infections / Q000175:diagnosis
chemical_list: D004279:DNA, Viral; D009067:Mouthwashes