title: Characterization and Biological Evaluation of Diterpenoids from Casearia graveolens.
abstract: Biologically active substances that promote the neurite outgrowth of nerve cells against neuron degeneration may be useful for the treatment of Alzheimer's disease. In a continuing search for bioactive compounds from plants, an ethyl acetate-soluble extract of the twigs of Casearia graveolens showed moderate stimulatory activity of neurite outgrowth from PC12 cells. Further investigation to obtain bioactive compounds led to the isolation of 10 new clerodane diterpenoids, graveopenes A-J (1-10). Their structures including absolute configurations were elucidated based on analysis of their NMR spectroscopic data and experimental and calculated ECD spectra. Compounds 3-6 and 8 were shown to stimulate NGF-mediated neurite outgrowth from PC12 cells.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Harbin University of Commerce , Harbin 150076, People's Republic of China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Ji
authors.forename: Xiaocong
authors.initials: X
authors.lastname: Sun
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Cao
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University , Sendai 980-8578, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ohizumi
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D037821:Casearia / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D020932:Nerve Growth Factor / Q000187:drug effects; D016501:Neurites / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane; D004365:Drugs, Chinese Herbal; D020932:Nerve Growth Factor