title: On-line Ultrasound-Assisted Dispersive Micro-Solid-Phase Extraction Based on Amino Bimodal Mesoporous Silica Nanoparticles for the Preconcentration and Determination of Cadmium in Human Biological Samples.
abstract: On-line ultrasound-assisted dispersive micro-solid-phase extraction (USA-DμSPE) has been developed for preconcentration and separation of trace amounts of Cd(II) ions in 0.5 mL of human biological samples. In a syringe with a nylon membrane, new synthetic bulky amino bimodal mesoporous silica nanoparticles (NH2-UVM7) were dispersed as a nanoadsorbent in 5 mL of diluted serum sample (1:10), and after ultrasonic shaking, the liquid phase was separated from the solid phase. At the optimized pH, the chemical and physical adsorption of cadmium ions occurred, respectively, based on complexation with amine groups of UVM7 (Cd:NH2-UVM7) and silica nanoparticles. The analyte was then back-extracted from the sorbent with nitric acid solution (0.2 M), and its concentration was determined by electrothermal atomic absorption spectrometry (ETAAS). Under the optimized conditions, the linear range, limit of detection (LOD), and preconcentration factor (PF) were obtained as 0.01-0.56 μg L(-1), 0.002 μg L(-1), and 25, respectively. The adsorption capacity of NH2-UVM7 was found to be 108.6 mg g(-1) of cadmium. The validation of the methodology was performed by the human standard reference material (HSRM).
authors.affiliation: Occupational and Environmental Health Research Center (OEHRC), Iranian Petroleum Industry Health Research Institute (IPIHRI), Tehran, 1485733111, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Shirkhanloo
authors.affiliation: Department of Chemistry, Semnan University, Semnan, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Falahnejad
authors.affiliation: Department of Chemistry, Semnan University, Semnan, Iran. hzmousavi@semnan.ac.ir.
authors.forename: H
authors.initials: H
authors.lastname: Zavvar Mousavi
mesh_terms: D000327:Adsorption; D002104:Cadmium / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020407:Internet; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D052616:Solid Phase Extraction; D013054:Spectrophotometry, Atomic; D013499:Surface Properties; D000069453:Ultrasonic Waves
chemical_list: D002104:Cadmium; D012822:Silicon Dioxide
keywords: Amino bimodal mesoporous silica nanoparticles; Cadmium; Electrothermal atomic absorption spectrometry; Human biological samples; Ultrasound-assisted dispersive micro-solid-phase extraction