title: Construction of a gE-Deleted Pseudorabies Virus and Its Efficacy to the New-Emerging Variant PRV Challenge in the Form of Killed Vaccine.
abstract: The new-emerging PRV variants plague the vaccinated pigs and caused huge economic loss to local pig industry in China since 2011. The current commercial PRV vaccines cannot provide complete protection as the new-emerging PRV variants are antigenically different from the classical viruses. It is urgent to develop more safe and effective PRV vaccines based on the current circulating field isolates. In this study, a gE gene-deleted PRV based on the PRV HN1201, a representative PRV variant, was generated and the efficacy was tested on 3-week-old pigs in the form of killed vaccine. After fatal PRV HN1201 challenge, all vaccinated pigs survived without showing any clinical symptoms, but all unvaccinated pigs exhibited pseudorabies-specific respiratory and neurological signs with 100% mortality rate within 6 days after infection. The vaccinated pigs developed high level of gB and neutralizing antibodies after vaccination which may correlate to the protection provided by vaccine. Therefore, this gE gene-deleted PRV could be a promising vaccine candidate for the control of currently epidemic pseudorabies in China.
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Tongyan
authors.initials: T
authors.lastname: Wang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Yang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Sun
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Chaoling
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Yan
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Linghua
authors.initials: L
authors.lastname: Guo
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Gao
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Lilin
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Li
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Tan
authors.affiliation: National Research Center for Veterinary Medicine, Road Cuiwei, High-Tech District, Luoyang 471003, China ; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Kegong
authors.initials: K
authors.lastname: Tian
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D017353:Gene Deletion; D011557:Pseudorabies / Q000235:genetics* / Q000276:immunology*; D013552:Swine / Q000276:immunology / Q000821:virology; D014611:Vaccination / Q000379:methods; D015164:Vaccines, Inactivated / Q000235:genetics* / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology; D017201:Virus Shedding / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C089580:glycoprotein E, Suid herpesvirus 1