title: PCR array and protein array studies demonstrate that IL-1β (interleukin-1β) stimulates the expression and secretion of multiple cytokines and chemokines in human adipocytes.
abstract: The role of IL-1β in regulating the expression and secretion of cytokines and chemokines by human adipocytes was examined. Adipocytes were incubated with human IL-1β for 4 or 24 h. The expression of a panel of 84 cytokine/chemokine genes was probed using PCR arrays. IL-1β stimulated the expression of >30 cytokine/chemokine genes on the arrays; 15 showed >100-fold increases in mRNA at 4 or 24 h including CSF3, CXCL1, CXCL2, CXCL12 and IL8. CSF3 exhibited a 10,000-fold increase in mRNA at 4 h. ADIPOQ was among the genes whose expression was inhibited. Protein arrays were used to examine the secretion of cytokines/chemokines from adipocytes. IL-1β stimulated the secretion of multiple cytokines/chemokines including MCP-1, IL-8, IP-10, MIP-1α and MCP-4. The most responsive was IP-10, which exhibited a 5000-fold increase in secretion with IL-1β. IL-1β is likely to play a substantial role in stimulating the inflammatory response in human adipocytes in obesity.
authors.affiliation: a Zoology Department , College of Science, King Saud University , Riyadh , Saudi Arabia .|b Central Laboratory College of Science, King Saud University , Riyadh , Saudi Arabia , and.
authors.forename: Suliman Y
authors.initials: SY
authors.lastname: Alomar
authors.affiliation: c Clore Laboratory, Buckingham Institute for Translational Medicine, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Zaibi
authors.affiliation: c Clore Laboratory, Buckingham Institute for Translational Medicine, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Malgorzata A
authors.initials: MA
authors.lastname: Kępczyńska
authors.affiliation: c Clore Laboratory, Buckingham Institute for Translational Medicine, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Gentili
authors.affiliation: a Zoology Department , College of Science, King Saud University , Riyadh , Saudi Arabia .
authors.forename: Afrah
authors.initials: A
authors.lastname: Alkhuriji
authors.affiliation: a Zoology Department , College of Science, King Saud University , Riyadh , Saudi Arabia .
authors.forename: Lamjad
authors.initials: L
authors.lastname: Mansour
authors.affiliation: b Central Laboratory College of Science, King Saud University , Riyadh , Saudi Arabia , and.
authors.forename: Javid Ahmed
authors.initials: JA
authors.lastname: Dar
authors.affiliation: a Zoology Department , College of Science, King Saud University , Riyadh , Saudi Arabia .|c Clore Laboratory, Buckingham Institute for Translational Medicine, University of Buckingham , Buckingham , United Kingdom.
authors.forename: Paul
authors.initials: P
authors.lastname: Trayhurn
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D040081:Protein Array Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018925:Chemokines; D016207:Cytokines; D053583:Interleukin-1beta; D012333:RNA, Messenger
keywords: Adipose tissue; gene expression; inflammation; obesity