title: Bio-mediated synthesis, characterization and cytotoxicity of gold nanoparticles.
abstract: We report here a "green" approach for the synthesis of gold nanoparticles (GNPs) in which the Mentha piperita extract was applied for the bioreduction of chloroauric acid and the stabilization of the formed nanostructures. The obtained GNPs were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy (TEM). The reduction of gold ions with the plant extract leads to the production of nanoparticles with various shapes (spherical, triangular and hexagonal) and sizes (from 10 to 300 nm). The kinetics of the reaction was monitored and various conditions of the synthesis were investigated. As a result, we established protocols optimized towards the synthesis of nanospheres and nanoprisms of gold. The cytotoxic effect of the obtained gold nanoparticles was studied by performing MTT assay, which showed lower cytotoxicity of the biosynthesized GNPs compared to gold nanorods synthesized using the usual seed-mediated growth. The results suggest that the synthesis using plant extracts may be a useful method to produce gold nanostructures for various biological and medical applications.
authors.affiliation: Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland. katarzyna.matczyszyn@pwr.edu.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Klekotko
authors.affiliation: Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland. katarzyna.matczyszyn@pwr.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Matczyszyn
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Siednienko
authors.affiliation: Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland. katarzyna.matczyszyn@pwr.edu.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Olesiak-Banska
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pawlik
authors.affiliation: Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland. katarzyna.matczyszyn@pwr.edu.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Samoc
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002712:Chlorides / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D017612:Gold Compounds / Q000737:chemistry; D055772:Green Chemistry Technology; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D036142:Mentha piperita / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002712:Chlorides; D017612:Gold Compounds; D010936:Plant Extracts; D006046:Gold; C024568:gold tetrachloride, acid