title: Physicochemical properties and bioactivity of freeze-cast chitosan nanocomposite scaffolds reinforced with bioactive glass.
abstract: Chitosan based nanocomposite scaffolds were prepared by freeze casting method through blending constant chitosan concentration with different portions of synthesized bioactive glass nanoparticles (BGNPs). Transmission Electron Microscopy (TEM) image showed that the particles size of bioactive glass (64SiO2.28CaO.8P2O5) prepared by sol-gel method was approximately less than 20 nm. Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray Diffraction (XRD) analysis showed proper interfacial bonding between BGNPs and chitosan polymers. Scanning Electron Microscopy (SEM) images depicted a unidirectional structure with homogenous distribution of BGNPs among chitosan matrix associated with the absence of pure chitosan scaffold's wall pores after addition of only 10 wt.% BGNPs. As the BGNP content increased from 0 to 50 wt.%, the compressive strength and compressive module values increased from 0.034 to 0.419 MPa and 0.41 to 10.77 MPa, respectively. Biodegradation study showed that increase in BGNP content leads to growth of weight loss amount. The in vitro biomineralization studies confirmed the bioactive nature of all nanocomposites. Amount of 30 wt.% BGNPs represented the best concentration for absorption capacity and bioactivity behaviors.
authors.affiliation: Department of Nanotechnology & Advanced Materials, Materials & Energy Research Center, Karaj, P.O. Box: 13145-1659, Iran. Electronic address: m.pourhaghgouy@merc.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Pourhaghgouy
authors.affiliation: Department of Nanotechnology & Advanced Materials, Materials & Energy Research Center, Karaj, P.O. Box: 13145-1659, Iran. Electronic address: a-zamanian@merc.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Zamanian
authors.affiliation: Department of Orthopedic Surgery, AJA University of Medical Sciences, Tehran, Iran. Electronic address: moshahrezaee@yahoo.com.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Shahrezaee
authors.affiliation: Department of Nanotechnology & Advanced Materials, Materials & Energy Research Center, Karaj, P.O. Box: 13145-1659, Iran. Electronic address: miladpourbaghi@gmail.com.
authors.forename: Milad Pourbaghi
authors.initials: MP
authors.lastname: Masouleh
mesh_terms: D017610:Calcium Compounds / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D005615:Freezing; D005782:Gels / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D017553:Phosphorus Compounds / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D017610:Calcium Compounds; D005782:Gels; D010087:Oxides; D017553:Phosphorus Compounds; C012500:phosphorus pentoxide; D012822:Silicon Dioxide; D048271:Chitosan; C016538:lime
keywords: Bioactive glass nanoparticles; Bone tissue engineering; Chitosan; Freeze casting; Nanocomposite; Scaffold