title: Genome-scale reconstruction of Salinispora tropica CNB-440 metabolism to study strain-specific adaptation.
abstract: The first manually curated genome-scale metabolic model for Salinispora tropica strain CNB-440 was constructed. The reconstruction enables characterization of the metabolic capabilities for understanding and modeling the cellular physiology of this actinobacterium. The iCC908 model was based on physiological and biochemical information of primary and specialised metabolism pathways. The reconstructed stoichiometric matrix consists of 1169 biochemical conversions, 204 transport reactions and 1317 metabolites. A total of 908 structural open reading frames (ORFs) were included in the reconstructed network. The number of gene functions included in the reconstructed network corresponds to 20% of all characterized ORFs in the S. tropica genome. The genome-scale metabolic model was used to study strain-specific capabilities in defined minimal media. iCC908 was used to analyze growth capabilities in 41 different minimal growth-supporting environments. These nutrient sources were evaluated experimentally to assess the accuracy of in silico growth simulations. The model predicted no auxotrophies for essential amino acids, which was corroborated experimentally. The strain is able to use 21 different carbon sources, 8 nitrogen sources and 4 sulfur sources from the nutrient sources tested. Experimental observation suggests that the cells may be able to store sulfur. False predictions provided opportunities to gain new insights into the physiology of this species, and to gap fill the missing knowledge. The incorporation of modifications led to increased accuracy in predicting the outcome of growth/no growth experiments from 76 to 93%. iCC908 can thus be used to define the metabolic capabilities of S. tropica and guide and enhance the production of specialised metabolites.
authors.affiliation: Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering, CeBiB, University of Chile, Beauchef 850, Santiago, Chile.
authors.forename: C A
authors.initials: CA
authors.lastname: Contador
authors.affiliation: Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering, CeBiB, University of Chile, Beauchef 850, Santiago, Chile.
authors.forename: V
authors.initials: V
authors.lastname: Rodríguez
authors.affiliation: Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering, CeBiB, University of Chile, Beauchef 850, Santiago, Chile.
authors.forename: B A
authors.initials: BA
authors.lastname: Andrews
authors.affiliation: Department of Chemical Engineering and Biotechnology, Centre for Biotechnology and Bioengineering, CeBiB, University of Chile, Beauchef 850, Santiago, Chile. juasenjo@ing.uchile.cl.
authors.forename: J A
authors.initials: JA
authors.lastname: Asenjo
mesh_terms: D000220:Adaptation, Biological; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods; D008848:Micromonosporaceae / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D010641:Phenotype; D015203:Reproducibility of Results
keywords: Genome-scale metabolic reconstruction; Metabolic capabilities; Salinispora tropica; Strain adaptation