title: Brain Natriuretic Peptide Stimulates Lipid Metabolism through Its Receptor NPR1 and the Glycerolipid Metabolism Pathway in Chicken Adipocytes.
abstract: Brain natriuretic peptide (BNP) is related to lipid metabolism in mammals, but its effect and the molecular mechanisms underlying it in chickens are incompletely understood. We found that the level of natriuretic peptide precursor B (NPPB, which encodes BNP) mRNA expression in high-abdominal-fat chicken groups was significantly higher than that of low-abdominal-fat groups. Partial correlations indicated that changes in the weight of abdominal fat were positively correlated with NPPB mRNA expression level. In vitro, compared with the control group, preadipocytes with NPPB interference showed reduced levels of proliferation, differentiation, and glycerin in media. Treatments of cells with BNP led to enhanced proliferation and differentiation of cells and glycerin concentration, and mRNA expression of its receptor natriuretic peptide receptor 1 (NPR1) was upregulated significantly. In cells exposed to BNP, 482 differentially expressed genes were identified compared with controls without BNP. Four genes known to be related to lipid metabolism (diacylglycerol kinase; lipase, endothelial; 1-acylglycerol-3-phosphate O-acyltransferase 1; and 1-acylglycerol-3-phosphate O-acyltransferase 2) were enriched in the glycerolipid metabolism pathway and expressed differentially. In conclusion, BNP stimulates the proliferation, differentiation, and lipolysis of preadipocytes through upregulation of the levels of expression of its receptor NPR1 and key genes enriched in the glycerolipid metabolic pathway.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|Institute of Poultry Science, Chinese Academy of Agriculture Sciences , Jiangsu 225125, P. R. China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|State Key Laboratory of Animal Nutrition , Beijing 100193, P. R. China.
authors.forename: G P
authors.initials: GP
authors.lastname: Zhao
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|State Key Laboratory of Animal Nutrition , Beijing 100193, P. R. China.
authors.forename: R R
authors.initials: RR
authors.lastname: Liu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|State Key Laboratory of Animal Nutrition , Beijing 100193, P. R. China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Li
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|State Key Laboratory of Animal Nutrition , Beijing 100193, P. R. China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Zheng
authors.affiliation: Institute of Poultry Science, Chinese Academy of Agriculture Sciences , Jiangsu 225125, P. R. China.
authors.forename: S F
authors.initials: SF
authors.lastname: Li
authors.affiliation: Institute of Poultry Science, Chinese Academy of Agriculture Sciences , Jiangsu 225125, P. R. China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Institute of Poultry Science, Chinese Academy of Agriculture Sciences , Jiangsu 225125, P. R. China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhao
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing 100193, P. R. China.|State Key Laboratory of Animal Nutrition , Beijing 100193, P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Wen
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002645:Chickens; D006017:Glycolipids / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D008066:Lipolysis; D053858:Metabolic Networks and Pathways; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D030161:Avian Proteins; D006017:Glycolipids; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A