title: Detection of Aeromonas hydrophila DNA oligonucleotide sequence using a biosensor design based on Ceria nanoparticles decorated reduced graphene oxide and Fast Fourier transform square wave voltammetry.
abstract: A new strategy was introduced for ssDNA immobilization on a modified glassy carbon electrode. The electrode surface was modified using polyaniline and chemically reduced graphene oxide decorated cerium oxide nanoparticles (CeO2NPs-RGO). A single-stranded DNA (ssDNA) probe was immobilized on the modified electrode surface. Fast Fourier transform square wave voltammetry (FFT-SWV) was applied as detection technique and [Ru(bpy)3](2+/3+) redox signal was used as electrochemical marker. The hybridization of ssDNA with its complementary target caused a dramatic decrease in [Ru(bpy)3](2+/3+) FFT-SW signal. The proposed electrochemical biosensor was able to detect Aeromonas hydrophila DNA oligonucleotide sequence encoding aerolysin protein. Under optimal conditions, the biosensor showed excellent selectivity toward complementary sequence in comparison with noncomplementary and two-base mismatch sequences. The dynamic linear range of this electrochemical DNA biosensor for detecting 20-mer oligonucleotide sequence of A. hydrophila was from 1 × 10(-15) to 1 × 10(-8) mol L(-1). The proposed biosensor was successfully applied for the detection of DNA extracted from A. hydrophila in fish pond water up to 0.01 μg mL(-1) with RSD of 5%. Besides, molecular docking was applied to consider the [Ru(bpy)3](2+/3+) interaction with ssDNA before and after hybridization.
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran.
authors.forename: Safiye
authors.initials: S
authors.lastname: Jafari
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran; Biosensor Research Center, Endocrinology & Metabolism Molecular and Cellular Research Institute, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: faridbodf@khayam.ut.ac.ir.
authors.forename: Farnoush
authors.initials: F
authors.lastname: Faridbod
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran; Biosensor Research Center, Endocrinology & Metabolism Molecular and Cellular Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Norouzi
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran.
authors.forename: Amin Shiralizadeh
authors.initials: AS
authors.lastname: Dezfuli
authors.affiliation: School of Chemistry, Damghan University, Damghan, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Ajloo
authors.affiliation: Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mohammadipanah
authors.affiliation: Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran; Biosensor Research Center, Endocrinology & Metabolism Molecular and Cellular Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques; D002563:Cerium / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D005583:Fourier Analysis; D006108:Graphite / Q000737:chemistry*; D062105:Molecular Docking Simulation; D053758:Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D004269:DNA, Bacterial; D010087:Oxides; D002563:Cerium; C030583:ceric oxide; D006108:Graphite
keywords: Aeromonas hydrophila; Biosensor; Cerium oxide; Fast Fourier transforms; Graphene oxide; Square wave voltammetry