title: The role of N-Acetylcysteine in preventing cyclophosphamide-induced gonadotoxicity: An experimental study in rats.
abstract: This study assessed whether NAC could prevent cyclophosphamide (CY)-induced damage, by measuring the anti-Müllerian hormone (AMH) levels. Forty-eight Sprague-Dawley female rats were divided into four groups: CY + NAC, CY, NAC and control, each including 12 rats. There was no significant difference among the 24-h AMH values of the groups (p = 0.452), whereas a significant difference was found in terms of 72-h values (p = 0.003). Paired comparisons revealed no significant difference between CY and CY + NAC (p>0.699) and NAC (p = 0.065) groups regarding 72-h AMH values. However, AMH concentrations of the CY group at 72 hours were significantly lower than those of the control group (p = 0.015). AMH concentrations of the CY + NAC group at 72 hours were also significantly lower than those of the NAC group (p = 0.002) and the control group (p = 0.002). The AMH levels of CY and CY + NAC groups at 72 hours were significantly lower than those at 24 hours. The 24-h and 72-h AMH levels in the NAC and control groups were similar. In the present study, a single dose of NAC failed to prevent the cytotoxic effects of CY.
authors.affiliation: a Department of Gynecology and Obstetrics Istanbul , Necmi Ayanoğlu Silivri State Hospital , Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Unal
authors.affiliation: b Department of Gynecology and Obstetrics Istanbul , Istanbul University Cerrahpasa Faculty of Medicine , Turkey.
authors.forename: M Aytac
authors.initials: MA
authors.lastname: Yuksel
authors.affiliation: c Department of Gynecology and Obstetrics , Istanbul Training and Research Hospital , Istanbul , Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Boran
authors.affiliation: d Department of Gynecology and Obstetrics , Okmeydanı Training and Research Hospital , Istanbul , Turkey.
authors.forename: I Temel
authors.initials: IT
authors.lastname: Yuksel
authors.affiliation: e Department of Gynecology and Obstetrics , Namik Kemal University Faculty of Medicine , Tekirdag , Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Abali
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D018906:Antineoplastic Agents, Alkylating / Q000009:adverse effects*; D003520:Cyclophosphamide / Q000009:adverse effects*; D004353:Drug Evaluation, Preclinical; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D007247:Infertility, Female / Q000139:chemically induced / Q000517:prevention & control*; D065851:Ovarian Reserve / Q000187:drug effects; D017207:Rats, Sprague-Dawley
chemical_list: D018906:Antineoplastic Agents, Alkylating; D016166:Free Radical Scavengers; D054304:Anti-Mullerian Hormone; D003520:Cyclophosphamide; D005978:Glutathione; D000111:Acetylcysteine
keywords: N-Acetylcysteine; anti-Müllerian hormone; cyclophosphamide; gonadotoxicity