title: CBLB502, an agonist of Toll-like receptor 5, has antioxidant and scavenging free radicals activities in vitro.
abstract: The bacterial protein flagellin is the known agonist of Toll-like receptor 5 (TLR5). It has been reported that CBLB502, a novel agonist of TLR5 derived from Salmonella flagellin, could reduce radiation toxicity in mouse and primate models, protect mice from dermatitis and oral mucositis caused by radiation, inhibit acute renal ischemic failure, and inhibit the growth of A549 lung cancer cell. The property of CBLB502 is able to bind to TLR5 and activates NF-κB signaling. In this study, we investigated the antioxidant potential and free radicals scavenging properties of CBLB502 in vitro. Interestingly, we found that CBLB502 has a direct and distinct antioxidant capacity and can efficiently scavenge a variety of free radicals, including superoxide anion, hydroxyl radical, and ABTS cation (ABTS(+)). Through wave scanning and kinetic evaluation of scavenging ABTS(+), we found that the ABTS(+) scavenging process of CBLB502 is relatively slow, and the ABTS(+) scavenging activity of CBLB502 has a consistently kinetics characteristics. In conclusion, our results suggested that CBLB502 has antioxidant and scavenging free radicals activities in vitro. It is implied that CBLB502 might partially promote the beneficial protective effect through its scavenging free radicals.
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Li
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Ge
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Wang
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Ophthalmology, General Hospital of Chinese PLA, Beijing 100853, China. Electronic address: 2468li@sina.com.
authors.forename: Zhaoyan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China. Electronic address: zhangcg@bmi.ac.cn.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D003001:Cloning, Molecular; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005609:Free Radicals / Q000037:antagonists & inhibitors; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D051198:Toll-Like Receptor 5 / Q000819:agonists*
chemical_list: D000975:Antioxidants; C528306:CBLB502; D016166:Free Radical Scavengers; D005609:Free Radicals; D010455:Peptides; D017382:Reactive Oxygen Species; D051198:Toll-Like Receptor 5
keywords: Antioxidant; CBLB502; Kinetics; Scavenging free radical; Toll-like receptor 5 agonist