title: Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels.
abstract: Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. Hypothesizing that a scaffold-free TEBV may be advantageous, we constructed a tubular structure (1 mm i.d.) from aligned human mesenchymal cell sheets (hMSC) as the wall and human endothelial progenitor cell (hEPC) coating as the lumen. The burst pressure of the scaffold-free TEBV was above 200 mmHg after three weeks of sequential culture in a rotating wall bioreactor and perfusion at 6.8 dynes/cm(2). The interwoven organization of the cell layers and extensive extracellular matrix (ECM) formation of the hMSC-based TEBV resembled that of native blood vessels. The TEBV exhibited flow-mediated vasodilation, vasoconstriction after exposure to 1 μM phenylephrine and released nitric oxide in a manner similar to that of porcine femoral vein. HL-60 cells attached to the TEBV lumen after TNF-α activation to suggest a functional endothelium. This study demonstrates the potential of a hEPC endothelialized hMSC-based TEBV for drug screening.
authors.affiliation: Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 130-650, Korea.
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.|Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: HaYeun
authors.initials: H
authors.lastname: Ji
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.|Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Zaozao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.|Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Hon
authors.initials: H
authors.lastname: Fai Chan
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Leigh
authors.initials: L
authors.lastname: Atchison
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Bruce
authors.initials: B
authors.lastname: Klitzman
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: George
authors.initials: G
authors.lastname: Truskey
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.|Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong
mesh_terms: D000818:Animals; D019149:Bioreactors; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D005109:Extracellular Matrix; D005268:Femoral Vein / Q000166:cytology / Q000378:metabolism; D018922:HL-60 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis / Q000378:metabolism; D010477:Perfusion; D010656:Phenylephrine / Q000494:pharmacology; D011312:Pressure; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D014409:Tumor Necrosis Factor-alpha; D010656:Phenylephrine; D009569:Nitric Oxide