title: Proton Channel Activity of Influenza A Virus Matrix Protein 2 Contributes to Autophagy Arrest.
abstract: Influenza A virus infection can arrest autophagy, as evidenced by autophagosome accumulation in infected cells. Here, we report that this autophagosome accumulation can be inhibited by amantadine, an antiviral proton channel inhibitor, in amantadine-sensitive virus infected cells or cells expressing influenza A virus matrix protein 2 (M2). Thus, M2 proton channel activity plays a role in blocking the fusion of autophagosomes with lysosomes, which might be a key mechanism for arresting autophagy.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong, China.
authors.forename: Yizhong
authors.initials: Y
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China chen_ling@gibh.ac.cn li_chufang@163.com.
authors.forename: Chufang
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong, China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Jiashun
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong, China.
authors.forename: Caijun
authors.initials: C
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong, China chen_ling@gibh.ac.cn li_chufang@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D001343:Autophagy; D054884:Host-Pathogen Interactions; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D011522:Protons; D014763:Viral Matrix Proteins / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D011522:Protons; D014763:Viral Matrix Proteins; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins