title: Rediscovery of Trypanosoma (Pycnomonas) suis, a tsetse-transmitted trypanosome closely related to T. brucei.
abstract: The African tsetse-transmitted trypanosomes are considered to be a well-known group of parasitic protozoa, but in 2008 a novel and distinctive trypanosome related to Trypanosoma brucei was discovered among tsetse isolates from Msubugwe in Tanzania. The host range, distribution and potential pathogenicity of this new trypanosome remain to be elucidated; such studies would be facilitated by a sensitive and specific identification method. Here, we identified two highly repetitive elements in the genome of the new trypanosome: a 177 bp repeat, which was located predominantly on the highly abundant minichromosomes, and a 138 bp repeat, which was widely dispersed in the genome. A PCR test based on each repeat was specific for the new trypanosome and sensitive to <0.1 trypanosome equivalent. These PCR tests were used to identify trypanosomes in archival pig blood smears from the 1950's, confirming the identity of the Msubugwe trypanosome as Trypanosoma (Pycnomonas) suis. We also present data on the molecular karyotype and spliced leader (SL, miniexon) repeat of the new trypanosome, both of which distinguish T. suis from other, better-known African tsetse-transmitted trypanosomes. The rediscovery of T. suis opens new lines of research into the evolution and biology of the African trypanosomes.
authors.affiliation: School of Biological Sciences, Life Sciences Building, University of Bristol, Tyndall Avenue, Bristol BS8 1TQ, U.K.
authors.forename: Rachel
authors.initials: R
authors.lastname: Hutchinson
authors.affiliation: School of Biological Sciences, Life Sciences Building, University of Bristol, Tyndall Avenue, Bristol BS8 1TQ, U.K.. Electronic address: w.gibson@bristol.ac.uk.
authors.forename: Wendy
authors.initials: W
authors.lastname: Gibson
mesh_terms: D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan; D008969:Molecular Sequence Data; D020038:RNA, Spliced Leader; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D013636:Tanzania / Q000453:epidemiology; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000145:classification* / Q000235:genetics; D014353:Trypanosomiasis, African / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission*; D014370:Tsetse Flies / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D020038:RNA, Spliced Leader
keywords: Archival DNA; Minichromosome; Salivarian trypanosomes; Satellite DNA; Species specific identification; Spliced leader