title: MicroRNA-204 inhibits cell proliferation in T-cell acute lymphoblastic leukemia by down-regulating SOX4.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a group of small non-coding RNAs that play important roles in the pathogenesis of human diseases by negatively regulating gene expression. The aim of this study was to explore the effect of miR-204 on cell proliferation migration and invasion in T-cell acute lymphoblastic leukaemia (T-ALL).
  
  
  METHODS
  miR-204 expression was determined in bone marrow samples from 32 leukemia patients and 32 healthy controls by quantitative real-time PCR (qRT-PCR). The effect of miR-204 on cell proliferation was evaluated by CCK8 assay, cell migration and invasion were evaluated by transwell migration and invasion assays, In addition, the regulation of SOX4 by miR-204 was evaluated by luciferase reporter assay and western blot.
  
  
  RESULTS
  our results revealed that miR-204 was low expressed in T-ALL. Cell proliferation assay showed that the cell proliferation ability was inhibited by miR-204 mimics. Moreover, migration and invasion assay suggested that overexpression of miR-204 could significantly suppressed the migration and invasion ability of T-ALL cells. Luciferase reporter assay confirmed that miR-204 directly bound to the 3' untranslated region of SOX4, and western blot suggested that miR-204 inhibited the expression of SOX4 at the protein levels.
  
  
  CONCLUSIONS
  Our findings indicated that miR-204 negatively regulates SOX4 and inhibited proliferation, migration and invasion of T-ALL cell lines. Thus, miR-204 might represent a potential therapeutic target for T-ALL intervention.
authors.affiliation: Department of Hematology, Xinxiang Central Hospital Xinxiang 453000, Henan Province, China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Yin
authors.affiliation: Department of Hematology, Xinxiang Central Hospital Xinxiang 453000, Henan Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liang
authors.affiliation: Department of Hematology, Xinxiang Central Hospital Xinxiang 453000, Henan Province, China.
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Zhan
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D055751:SOXC Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*
chemical_list: C549404:MIRN204 microRNA, human; D035683:MicroRNAs; C083431:SOX4 protein, human; D055751:SOXC Transcription Factors
keywords: SOX4; T-cell acute lymphoblastic leukaemia; miR-204