title: Submicron polycaprolactone particles as a carrier for imaging contrast agent for in vitro applications.
abstract: Fluorescent materials have recently attracted considerable attention due to their unique properties and high performance as imaging agent in biomedical fields. Different imaging agents have been encapsulated in order to restrict its delivery to a specific area. In this study, a fluorescent contrast agent was encapsulated for in vitro application by polycaprolactone (PCL) polymer. The encapsulation was performed using modified double emulsion solvent evaporation technique with sonication. Fluorescent nanoparticles (20 nm) were incorporated in the inner aqueous phase of double emulsion. A number of samples were fabricated using different concentrations of fluorescent contrast agent. The contrast agent-containing submicron particle was characterized by a zetasizer for average particle size, SEM and TEM for morphology observations and fluorescence spectrophotometer for encapsulation efficiency. Moreover, contrast agent distribution in the PCL matrix was determined by confocal microscopy. The incorporation of contrast agent in different concentrations did not affect the physicochemical properties of PCL particles and the average size of encapsulated particles was found to be in the submicron range.
authors.affiliation: University of Lyon, University Lyon-1, CNRS, UMR 5007, LAGEP- CPE, 43 bd 11 Novembre 1918, Villeurbanne, Lyon F-69622, France.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Iqbal
authors.affiliation: Laboratory of Engineering and Cutaneous Biology, INSERM UMR 1098, Medicine and Pharmaceutical University, 19 rue Ambroise Paré, 25000 Besançon, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Robin
authors.affiliation: Laboratory of Engineering and Cutaneous Biology, INSERM UMR 1098, Medicine and Pharmaceutical University, 19 rue Ambroise Paré, 25000 Besançon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Humbert
authors.affiliation: Laboratory of Engineering and Cutaneous Biology, INSERM UMR 1098, Medicine and Pharmaceutical University, 19 rue Ambroise Paré, 25000 Besançon, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Viennet
authors.affiliation: University of Lyon, University Lyon-1, CNRS, UMR 5007, LAGEP- CPE, 43 bd 11 Novembre 1918, Villeurbanne, Lyon F-69622, France.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Agusti
authors.affiliation: University of Lyon, University Lyon-1, CNRS, UMR 5007, LAGEP- CPE, 43 bd 11 Novembre 1918, Villeurbanne, Lyon F-69622, France.
authors.forename: Hatem
authors.initials: H
authors.lastname: Fessi
authors.affiliation: University of Lyon, University Lyon-1, CNRS, UMR 5007, LAGEP- CPE, 43 bd 11 Novembre 1918, Villeurbanne, Lyon F-69622, France. Electronic address: elaissari@lagep.univ-lyon1.fr.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Elaissari
mesh_terms: D003287:Contrast Media / Q000008:administration & dosage*; D004337:Drug Carriers; D006801:Humans; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011091:Polyesters / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone
keywords: Contrast agent; Encapsulation; Fluorescent imaging; Morphology; Skin penetration; Submicron particles size