title: Rotavirus VP7 epitope chimeric proteins elicit cross-immunoreactivity in guinea pigs.
abstract: VP7 of group A rotavirus (RVA) contains major neutralizing epitopes. Using the antigenic protein VP6 as the vector, chimeric proteins carrying foreign epitopes have been shown to possess good immunoreactivity and immunogenicity. In the present study, using modified VP6 as the vector, three chimeric proteins carrying epitopes derived from VP7 of RVA were constructed. The results showed that the chimeric proteins reacted with anti-VP6 and with SA11 and Wa virus strains. Antibodies from guinea pigs inoculated with the chimeric proteins recognized VP6 and VP7 of RVA and protected mammalian cells from SA11 and Wa infection in vitro. The neutralizing activities of the antibodies against the chimeric proteins were significantly higher than those against the vector protein VP6F. Thus, development of chimeric vaccines carrying VP7 epitopes using VP6 as a vector could be a promising alternative to enhance immunization against RVAs.
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Bingxin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming, 650500, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Pan
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Wenyue
authors.initials: W
authors.lastname: Xia
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China. chenyd@imbcams.com.cn.
authors.forename: Yuanding
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D015870:Gene Expression; D005822:Genetic Vectors; D006168:Guinea Pigs; D008253:Macaca mulatta; D009500:Neutralization Tests; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D012400:Rotavirus Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C094777:SA-11 antigen, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus
keywords: VP6; VP7 epitope chimeric vaccine; foreign epitope presenting vector; neutralizing activity; rotavirus