title: Expression of RNAs Coding for Metal Transporters in Blood of Patients with Huntington's Disease.
abstract: Recent studies have demonstrated elevated levels of iron (Fe) in brains of patients with Huntington's disease (HD). Striatal cells carrying mutated Huntingtin presented increased sensitivity to cadmium (Cd) toxicity, decreased sensitivity to manganese (Mn) toxicity and deficits in Mn uptake. The hypothesis arose that the observed alterations result from the altered expression and/or activity of proteins engaged in the transport of these metals, that is: transferrin (TF), transferrin receptor (TFR), divalent metal transporter 1 (DMT1) and ZIP8 protein. Here we examined the expression levels of genes encoding these proteins in blood of HD patients and control subjects. A decreasing tendency in the level of TF transcript and increasing tendency of SLC11A2 mRNA encoding DMT1 was observed in the blood of HD patients compared to the control subjects, but neither attained statistical significance. No changes were found in the levels of TFRC coding for TFR and SLC39A8 coding for ZIP8 between HD patients and controls. The results indicate that HD-associated changes in metal homeostasis occur are not related to mechanisms other than the expression level of the here analyzed metal transporters.
authors.affiliation: Department of Neurotoxicology, Mossakowski Medical Research Centre Polish Academy of Sciences, 5 Pawińskiego Str., 02-106, Warsaw, Poland. mszeliga@imdik.pan.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Szeliga
authors.affiliation: Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, 166 Nowoursynowska Str., 02-787, Warsaw, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Różycka
authors.affiliation: Department of Molecular Biology, University of Gdańsk, 59 Wita Stwosza Str., 80-308, Gdańsk, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Jędrak
authors.affiliation: Department of Molecular Biology, University of Gdańsk, 59 Wita Stwosza Str., 80-308, Gdańsk, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Barańska
authors.affiliation: Department of Neurology, The Wolski Hospital im Dr Anny Gostyńskiej, 17 Kasprzaka Str., 01-211, Warsaw, Poland.|Department of Neurology, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Janik
authors.affiliation: Department of Neurology, Medical University of Warsaw, 1 Banacha Str., 02-097, Warsaw, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Jamrozik
authors.affiliation: Department of Neurotoxicology, Mossakowski Medical Research Centre Polish Academy of Sciences, 5 Pawińskiego Str., 02-106, Warsaw, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Albrecht
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000097:blood* / Q000235:genetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics*; D008670:Metals / Q000378:metabolism*; D008875:Middle Aged; D012313:RNA / Q000097:blood*
chemical_list: D026901:Membrane Transport Proteins; D008670:Metals; D012313:RNA
keywords: DMT1; Huntington’s disease; Metal transporter; TF; TFR; ZIP8