title: Effects of sialidase NEU1 siRNA on proliferation, apoptosis, and invasion in human ovarian cancer.
abstract: Ovarian cancer is one of the most common malignancies encountered in the world. In ovarian cancer tissues of patients, NEU1 was expressed in a higher level than that in adjacent normal tissues. In this research, we aimed to elucidate the role of NEU1 siRNA on proliferation, apoptosis, and invasion of OVCAR3 and SKOV3 cells which expressed NEU1 notably. By cell viability assay and flow cytometry method, we found that NEU1 siRNA effectively inhibited the cancer proliferation, arrested cells cycle at G0/G1 phase, and induced apoptosis when compared to the Mock group. Result of transwell assay showed that invasion of cells in OVCAR3 and SKOV3 treated with NEU1 siRNA were suppressed significantly. Gene set enrichment analysis showed that lysosome and oxidative phosphorylation related signal pathway were associated with the NEU1 expression. In addition, Western blot revealed that expressions of Cln3 and Cln5 were depressed, and ATP5B and ATP5J expressions were also reduced. In conclusion, NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment.
authors.affiliation: Department of Obstetrics and Gynaecology, Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen, Guangdong, 518133, People's Republic of China.
authors.forename: Li-rong
authors.initials: LR
authors.lastname: Ren
authors.affiliation: Department of Obstetrics and Gynaecology, Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen, Guangdong, 518133, People's Republic of China.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shu-ying
authors.initials: SY
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynaecology, Shenzhen Baoan Maternal and Child Health Hospital, Shenzhen, Guangdong, 518133, People's Republic of China. yuanfangzhu31@163.com.|Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China. yuanfangzhu31@163.com.
authors.forename: Yuan-fang
authors.initials: YF
authors.lastname: Zhu
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Wen-juan
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shan-yu
authors.initials: SY
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Nanchang University, 17 Yongwaizheng Street, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009439:Neuraminidase / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000473:pathology*; D011247:Pregnancy; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; C486385:NEU1 protein, human; D009439:Neuraminidase
keywords: Apoptosis; Invasion; NEU1; Ovarian cancer; Proliferation