title: L-periaxin interacts with S-periaxin through its PDZ domain.
abstract: Periaxin was first identified as a protein in myelinating Schwann cells through a screen of novel cytoskeleton-associated proteins in peripheral nerve myelination. The periaxin gene encodes two isoforms, namely, L- and S-periaxin, which are 1461 and 147 residues in size, respectively. Several loss-of-function mutations linked to autosomal recessive Dejerine-Sottas neuropathy and demyelinating Charcot-Marie-Tooth disease in periaxin have been described. In this study, the colocolization of L- and S-periaxin in the cytoplasm of RSC96 cells was found by immunofluorescence assays. The interaction between these two isoforms was confirmed by co-immunoprecipitation, fluorescence complementation experiment, and GST pull-down assay. Results also showed that the two periaxin isoforms interacted in the cytoplasm through the PDZ domain, and their interaction prevented the homodimerization of L-periaxin. S-periaxin may regulate the function of L-periaxin in Schwann cells.
authors.affiliation: Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, PR China. Electronic address: yangyenan@foxmail.com.
authors.forename: Yenan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, PR China. Electronic address: yaweishi@sxu.edu.cn.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D054731:PDZ Domains; D020033:Protein Isoforms / Q000378:metabolism; D055503:Protein Multimerization; D051381:Rats; D012583:Schwann Cells / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D020033:Protein Isoforms; C086745:periaxin
keywords: BiFC; Interaction; L-periaxin; PDZ domain; S-periaxin