title: BacMam Platform for Vaccine Antigen Delivery.
abstract: Recombinant baculo viruses based on Autographa californica multiple nuclear polyhedrosis virus carrying vertebrate cell active expression cassettes, so-called BacMam viruses, are increasingly used as gene delivery vectors for vaccination of animals against pathogens. Different approaches for generation of BacMams exist and a variety of transfer vectors to improve target protein expression in vivo have been constructed. Here we describe a use of transfer vector which contains an insect cell-restricted expression cassette for the green fluorescent protein and thus enables easy monitoring of BacMam virus rescue, fast plaque purification of recombinants and their convenient titer determination and which has been proven to be efficacious for gene delivery in vaccination/challenge experiments.
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany. guenther.keil@fli.bund.de.|Institut für Virusdiagnostik, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany. guenther.keil@fli.bund.de.
authors.forename: Günther M
authors.initials: GM
authors.lastname: Keil
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.|Institut für Virusdiagnostik, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.
authors.forename: Reiko
authors.initials: R
authors.lastname: Pollin
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.|Institut für Virusdiagnostik, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Müller
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.|Institut für Virusdiagnostik, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Giesow
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.|Institut für Virusdiagnostik, Friedrich-Loeffler-Instiut, Südufer 10, Greifswald, 17493, Insel Riems, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Schirrmeier
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D016367:Baculoviridae / Q000235:genetics* / Q000276:immunology; D015967:Gene Expression Regulation, Viral; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D009036:Moths / Q000166:cytology / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014161:Transduction, Genetic; D014162:Transfection; D014612:Vaccines / Q000235:genetics* / Q000276:immunology
chemical_list: D000941:Antigens; D014612:Vaccines; D049452:Green Fluorescent Proteins
keywords: BacMam technology; Baculo virus transfer plasmids; Green fluorescent protein expression; In vitro and in vivo transduction