title: Analgesic effects of NB001 on mouse models of arthralgia.
abstract: Our previous studies have demonstrated the critical roles of calcium-stimulated adenylyl cyclase 1 (AC1) in the central nervous system in chronic pain. In the present study, we examined the analgesic effects of NB001, a selective inhibitor of AC1, on animal models of ankle joint arthritis and knee joint arthritis induced by complete Freund's adjuvant injection. NB001 treatment had no effect on joint edema, stiffness, and joint destruction. Furthermore, the treatment failed to attenuate the disease progression of arthritis. However, NB001 treatment (3 mg/kg) significantly weakened joint pain-related behavior in the mouse models of ankle joint arthritis and knee joint arthritis. Results indicated that NB001 exhibited an analgesic effect on the animal models of arthritis but was not caused by anti-inflammatory activities.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Department of Orthopedics, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, Nanjing, 210002, China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Xin-shang
authors.initials: XS
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Guo
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.|Department of Pharmacy, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China. minggao@fmmu.edu.cn.
authors.forename: Ming-gao
authors.initials: MG
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an, 710032, China. liushb1974@aliyun.com.
authors.forename: Shui-bing
authors.initials: SB
authors.lastname: Liu
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D018771:Arthralgia / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D001522:Behavior, Animal; D004195:Disease Models, Animal; D005620:Freund's Adjuvant; D006930:Hyperalgesia / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology; D007249:Inflammation / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D007719:Knee Joint / Q000187:drug effects / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D010146:Pain / Q000150:complications / Q000188:drug therapy / Q000473:pathology; D012684:Sensory Thresholds
chemical_list: C557530:5-((2-(6-Amino-9H-purin-9-yl) ethyl) amino)-1-pentanol; D000700:Analgesics; D000255:Adenosine Triphosphate; D005620:Freund's Adjuvant