title: Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis.
abstract: Jianpi Huayu Decoction (JHD), a Chinese medicine formula, is a typical prescription against multiple tumors in the clinical treatment, which can raise quality of life and decrease complications. The aim of this study is to assess the efficacy of JHD against human colorectal carcinoma cells (SW480) and explore its mechanism. MTT assay showed that JHD decreased the cellular viability of SW480 cells in dose-dependent and time-dependent manner. Flow cytometry analysis revealed that JHD induced G0/G1-phase cell cycle arrest in SW480 cells and had a strong apoptosis-inducing effect on SW480 cells. Meanwhile it enhanced the expression of p27, cleaved PARP, cleaved caspase-3, and Bax and decreased the levels of PARP, caspase-3, Bcl-2, CDK2, CDK4, CDK6, cyclin D1, cyclin D2, cyclin D3, and cyclin E1, which was evidenced by RT-qPCR and Western blot analysis. In conclusion, these results indicated that JHD inhibited proliferation in SW480 cells by inducing G0/G1-phase cell cycle arrest and apoptosis, providing a practicaltherapeutic strategy against colorectal cancer.
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Song-Yang
authors.initials: SY
authors.lastname: Xi
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Yu-Hao
authors.initials: YH
authors.lastname: Teng
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Jie-Ping
authors.initials: JP
authors.lastname: Li
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China ; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Shen-Lin
authors.initials: SL
authors.lastname: Liu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zou
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, China.
authors.forename: Rui-Ping
authors.initials: RP
authors.lastname: Wang