title: [Changes of the Expression of Brain Derived Neurotrophic Factors in Rats Trachea Induced by Acrolein Exposure].
abstract: OBJECTIVE
  To investigate expressional changes of brain derived neurotrophic factor (BDNF) in the trachea of rats with acrolein inhalation.
  
  
  METHODS
  Twenty two SD rats were divided into 2 groups: the rats in experimental group were subjected to acrolein inhalation for the induce of trachea inflammatory injury, while the rats with saline (NS) inhalation were as control. All the rats were sacrificed in 1,3,6 weeks after acrolein (n = 11 at each time point) or saline inhalation (n = 11 at each time point), the samples of trachea epithelium were harvested. The immunohistochemistry and in situ hybridization was performed to detect the location of BDNF protein and mRNA in trachea. The expression of BDNF mRNA in the trachea tissues were determined by RT-PCR.
  
  
  RESULTS
  There are positive cells in epithelium of trachea for BDNF protein and mRNA, with cytoplasm staining. The expression of BDNF mRNA in the trachea was increased at 1 week after acrolein inhalation (P < 0.05, vs. control group), then decreased along with the time and reached to the same level as control group at 3 weeks, then last to 6 weeks (P > 0.05).
  
  
  CONCLUSIONS
  The inflammatory injury in trachea induced by acrolein exposure could be associated with the increased expression of BDNF. BDNF may be one of the crucial inflammatory factors in the process of inflammatory reaction in trachea with acrolein stimulation.
authors.forename: Bing
authors.initials: B
authors.lastname: Yuan
authors.forename: Rui-an
authors.initials: RA
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Xu
authors.forename: Qi-qin
authors.initials: QQ
authors.lastname: Dan
authors.forename: Yun-hui
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000171:Acrolein / Q000494:pharmacology*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D000171:Acrolein