title: T-CELL RESPONSES ASSESSED USING IGRA AND TST ARE NOT CORRELATED WITH AFB GRADE AND CHEST RADIOGRAPH IN PULMONARY TUBERCULOSIS PATIENTS.
abstract: A definitive marker determining the bacillary load of Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB), and hence disease severity, is required for patient monitoring and management. In this study, the association among T-cell responses based on the interferon-gamma release assay (IGRA) and the tuberculin skin test (TST), the sputum acid-fast bacilli (AFB) grade and types of radiological lesions were analyzed in new cases of pulmonary TB patients (n = 54) at Srinagarind Hospital, Khon Kaen, Thailand between September 1, 2012 and March 31, 2014. It was found that infiltrative and cavitary lesions from chest radiographs were associated with high sputum AFB grade (p = 0.048). T-cell responses from both IGRA and TST were not correlated with sputum AFB grade. Neither IGRA nor TST was correlated with the bacillary load as defined by AFB grade and chest radiographs. Patients with cavitary lesions on chest radiographs tended to have high IFN-γ concentrations and large TST indurations. In addition, TB patients with previous BCG vaccination showed significantly higher IFN-γ induction compared to the non-vaccinated group (p = 0.001). This study showed T-cell responses based on both IGRA and TST were not correlated with AFB grade and chest radiograph. In areas of high rates of BCG vaccination, as in Thailand, the BCG may affect IGRA and TST interpretations.
authors.forename: Kiatichai
authors.initials: K
authors.lastname: Faksri
authors.forename: Wipa
authors.initials: W
authors.lastname: Reechaipichitkul
authors.forename: Wilailuk
authors.initials: W
authors.lastname: Pimrin
authors.forename: Janpen
authors.initials: J
authors.lastname: Bourpoern
authors.forename: Supapim
authors.initials: S
authors.lastname: Prompinij
mesh_terms: D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods / Q000706:statistics & numerical data*; D005260:Female; D006801:Humans; D059425:Interferon-gamma Release Tests / Q000706:statistics & numerical data*; D008297:Male; D008875:Middle Aged; D013902:Radiography, Thoracic / Q000706:statistics & numerical data*; D013183:Sputum / Q000382:microbiology; D013785:Thailand; D014374:Tuberculin Test / Q000706:statistics & numerical data; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology* / Q000473:pathology*