title: Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging.
abstract: In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 µg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 µg/mL. High concentrations (≥250 µg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 µg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 µM, reaching 80% inhibition at 125 µM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Ehn-Kyoung
authors.initials: EK
authors.lastname: Choi
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Guo
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, Korea.
authors.forename: Jae-Kwon
authors.initials: JK
authors.lastname: Choi
authors.affiliation: Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Korea.
authors.forename: Su-Kil
authors.initials: SK
authors.lastname: Jang
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Kyungha
authors.initials: K
authors.lastname: Shin
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Ye-Seul
authors.initials: YS
authors.lastname: Cha
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Choi
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Da-Woom
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Central Research Institute, Dr. Chung's Food Co. Ltd., Cheongju, Korea.
authors.forename: Yoon-Bok
authors.initials: YB
authors.lastname: Lee
authors.affiliation: Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, Korea.
authors.forename: Seong-So
authors.initials: SS
authors.lastname: Joo
authors.affiliation: College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: Yun-Bae
authors.initials: YB
authors.lastname: Kim
keywords: Rosa hybrida; elastase; matrix metalloproteinase-1; tyrosinase; white rose petal extract