title: Coculture of Primary Motor Neurons and Schwann Cells as a Model for In Vitro Myelination.
abstract: A culture system that can recapitulate myelination in vitro will not only help us better understand the mechanism of myelination and demyelination, but also find out possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible myelination culture system using mouse motor neurons (MNs) and Schwann cells (SCs). Dissociated motor neurons are plated on a feeder layer of SCs, which interact with and wrap around the axons of MNs as they differentiate in culture. In our MN-SC coculture system, MNs survived over 3 weeks and extended long axons. Both viability and axon growth of MNs in the coculture were markedly enhanced as compared to those of MN monoculture. Co-labeling of myelin basic proteins (MBPs) and neuronal microtubules revealed that SC formed myelin sheaths by wrapping around the axons of MNs. Furthermore, using the coculture system we found that treatment of an antioxidant substance coenzyme Q10 (Co-Q10) markedly facilitated myelination.
authors.affiliation: Center for Bionics, Korea Institute of Science and Technology (KIST), Seoul, Korea.|Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.
authors.forename: Sujin
authors.initials: S
authors.lastname: Hyung
authors.affiliation: Center for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul, Korea.|Department of Neuroscience, Korea University of Science and Technology, Daejeon, Korea.
authors.forename: Bo
authors.initials: B
authors.lastname: Yoon Lee
authors.affiliation: Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
authors.affiliation: Center for Bionics, Korea Institute of Science and Technology (KIST), Seoul, Korea.
authors.forename: Jinseok
authors.initials: J
authors.lastname: Kim
authors.affiliation: Center for Neuroscience, Korea Institute of Science and Technology (KIST), Seoul, Korea.|Department of Neuroscience, Korea University of Science and Technology, Daejeon, Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Hur
authors.affiliation: Center for Bionics, Korea Institute of Science and Technology (KIST), Seoul, Korea.
authors.forename: Jun-Kyo
authors.initials: JK
authors.lastname: Francis Suh
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D018920:Coculture Techniques; D061252:Feeder Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D008954:Models, Biological; D009046:Motor Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D055757:SOXE Transcription Factors / Q000235:genetics / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D015415:Biomarkers; C499047:Mbp protein, mouse; D004676:Myelin Basic Protein; D055757:SOXE Transcription Factors; C527924:Sox10 protein, mouse; D014451:Ubiquinone; C024989:coenzyme Q10