title: Characterization of plasma protein binding dissociation with online SPE-HPLC.
abstract: A novel parameter of relative recovery (R re) was defined and determined by online SPE-HPLC to characterize plasma protein binding (PPB) kinetics of highly plasma binding drugs. The proportional relationship of R re with k off of PPB has been established with a new SPE model. A rapid, easy to use method could potentially be used to categorize PK properties of the drug candidates in the decision process of drug discovery and development.
authors.affiliation: College of Pharmacy &State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, P.R.China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, P.R.China.|Tianjin International Joint Academy of Biomedicine, Tianjin 300457, P.R.China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Pharmacy &State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, P.R.China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, P.R.China.|Tianjin International Joint Academy of Biomedicine, Tianjin 300457, P.R.China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Mathematical Sciences, Nankai University, Tianjin 300071, P.R.China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Pharmacy &State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, P.R.China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, P.R.China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: College of Pharmacy &State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, P.R.China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, P.R.China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yin
mesh_terms: D001665:Binding Sites; D001798:Blood Proteins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D001798:Blood Proteins; D004364:Pharmaceutical Preparations