title: Enhancement of Cellulase and Xylanase Production Using pH-Shift and Dissolved Oxygen Control Strategy with Streptomyces griseorubens JSD-1.
abstract: In this study, the production of cellulase and xylanase by Streptomyces griseorubens JSD-1 was improved by integrating the pH-shift and dissolved oxygen (DO)-constant control strategies. The pH-shift control strategy was carried out by analyzing the specific cell growth rate (μ) and specific enzyme formation rate (Q p) of S. griseorubens JSD-1. The pH was controlled at 8.0 during the first 48 h to maintain high cell growth, which then shifted to 7.5 after 48 h to improve the production of cellulase and xylanase. Using this method, the maximum activities of cellulase, xylanase, and filter paper enzyme (FPase) increased by 47.9, 29.5, and 113.6 %, respectively, compared to that obtained without pH control. On the basis of pH-shift control, the influence of DO concentrations on biomass and enzyme production was further investigated. The maximum production of cellulase, xylanase, and FPase reached 114.38 ± 0.96 U mL(-1), 330.57 ± 2.54 U mL(-1), and 40.11 ± 0.38 U mL(-1), which were about 1.6-fold, 0.6-fold, and 3.2-fold higher than that of neutral pH without DO control conditions. These results supplied a functional approach for improving cellulase and xylanase production.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Luo
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China.
authors.forename: Yuee
authors.initials: Y
authors.lastname: Zhi
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People's Republic of China. peizhousjtu@163.com.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People's Republic of China. peizhousjtu@163.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhou
mesh_terms: D019149:Bioreactors; D002480:Cellulase / Q000096:biosynthesis*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism*; D012995:Solubility; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002480:Cellulase; D043364:Endo-1,4-beta Xylanases; D010100:Oxygen
keywords: Cellulase; Dissolved oxygen; Kinetic parameters; Xylanase; pH control