title: Cartilage Oligomeric Matrix Protein-Angiopoietin-1 Has a Protective Effect of Vascular Endothelial Barrier in Rat With Acute Necrotizing Pancreatitis.
abstract: OBJECTIVE
  To investigate the protective effect of angiopoietin-1 (Ang-1) from capillary endothelial damage in rats with acute necrotizing pancreatitis (ANP).
  
  
  METHODS
  96 male Sprague-Dawley rats were randomly averaged and divided into control group, ANP group, Si-Ang-1 group, and COMP (cartilage oligomeric matrix protein)-Ang-1 group. Animals were killed at 6, 12, and 24 hours after molding. Levels of serum amylase, porcine endothelin 1, C-reactive protein, and Ang-1 were detected; histopathological changes in the pancreas were observed; capillary permeability and Ang-1 expression of the pancreatic tissue were detected by Evans Blue extravasation assay, immunohistochemistry, Western blot, and quantitative polymerase chain reaction.
  
  
  RESULTS
  (1) Levels of serum amylase, C-reactive protein, and porcine endothelin-1 increased and level of Ang-1 decrease in the ANP group and Si-Ang-1 group compared with the control group, whereas COMP-Ang-1 group could improve the changes. (2) The order of pancreas pathological changes (mild to severe) is: control group, COMP-Ang-1 group, ANP group, and Si-Ang-1 group. (3) Capillary permeability of the pancreatic tissue in the COMP-Ang-1 group was lower than that in the ANP group. (4) Ang-1 mRNA and protein expression in the COMP-Ang-1 group was significantly higher than in the ANP group.
  
  
  CONCLUSIONS
  COMP-Ang-1 can upregulate the expression of Ang-1 protein to promote angiogenesis and improve early inflammatory and pathological damage in ANP group.
authors.affiliation: From the *Department of General Surgery and †Laboratory Center, Putuo Hospital, Shanghai University of Traditional Chinese Medicine Shanghai, China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Chen
authors.forename: Ping-Ting
authors.initials: PT
authors.lastname: Kong
authors.forename: Hong-Chang
authors.initials: HC
authors.lastname: Li
authors.forename: Xin-Juan
authors.initials: XJ
authors.lastname: Fan
authors.forename: Jia-Min
authors.initials: JM
authors.lastname: Tu
authors.forename: Jin-Kun
authors.initials: JK
authors.lastname: Xie
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Tian
authors.forename: Li-Yun
authors.initials: LY
authors.lastname: Pan
authors.forename: Teng
authors.initials: T
authors.lastname: Chen
authors.forename: Yi-Jun
authors.initials: YJ
authors.lastname: Cao
authors.forename: Pei-Hao
authors.initials: PH
authors.lastname: Yin
authors.forename: Wen
authors.initials: W
authors.lastname: Peng
authors.forename: Dian-Xu
authors.initials: DX
authors.lastname: Feng
mesh_terms: D000681:Amylases / Q000097:blood; D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D042683:Angiopoietin-1 / Q000097:blood / Q000235:genetics; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002097:C-Reactive Protein / Q000378:metabolism; D002199:Capillary Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D008297:Male; D018919:Neovascularization, Physiologic; D010179:Pancreas / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D043925:Angiogenesis Inducing Agents; D042683:Angiopoietin-1; C467629:Angpt1 protein, rat; D000893:Anti-Inflammatory Agents; C515576:COMP-Ang1 fusion protein; D019332:Endothelin-1; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D002097:C-Reactive Protein; D000681:Amylases