title: Competitive immunochromatographic assay for leptosperin as a plausible authentication marker of manuka honey.
abstract: Manuka honey is known as one of the premium honeys because of its unique property: a potent antibacterial activity. Leptosperin, methyl syringate 4-O-β-d-gentiobioside, has been specifically identified in manuka honey. Because leptosperin is relatively stable under warmer conditions, measuring leptosperin levels may be applied to authenticate manuka honey. In this study, an immunochromatographic separation and quantification of leptosperin techniques have been developed. The concentration of leptosperin measured by immunochromatography was significantly correlated with the concentration measured by high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA). Because the immunochromatographic method is rapid and reliable, it could be applied to on-site quality control or inspection of honey samples by a beekeeper, a manufacturer, an inspector, a retailer, or a consumer.
authors.affiliation: School of Human Science and Environment, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan; Research Institute for Food and Nutritional Sciences, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan. Electronic address: yojikato@shse.u-hyogo.ac.jp.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Kato
authors.affiliation: School of Human Science and Environment, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Araki
authors.affiliation: School of Human Science and Environment, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Juri
authors.affiliation: School of Human Science and Environment, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan; Research Institute for Food and Nutritional Sciences, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan.
authors.forename: Akari
authors.initials: A
authors.lastname: Ishisaka
authors.affiliation: Graduate School of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja-shi, Okayama 719-1197, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nitta
authors.affiliation: Faculty of Health and Nutrition, Shubun University, 6, Nikkocho, Ichinomiya, Aichi 491-0938, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Niwa
authors.affiliation: School of Human Science and Environment, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan; Research Institute for Food and Nutritional Sciences, University of Hyogo, 1-1-12, Shinzaike-honcho, Himeji, Hyogo 670-0092, Japan.
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Kitamoto
authors.affiliation: Healthcare Systems, Inc., 2-22-8, Chikusa-ku, Nagoya, Aichi 464-0858, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takimoto
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006027:Glycosides / Q000737:chemistry*; D006722:Honey / Q000032:analysis*; D031649:Leptospermum / Q000737:chemistry*; D011786:Quality Control
chemical_list: D006027:Glycosides
keywords: Glycoside; Immunochromatography; Leptospermum; Quality control