title: Chemical strategies and tandem mass spectrometry for the detection and identification of protein modifications by electrophilic lipoxidation products.
abstract: The post-translational modification of proteins by electrophilic oxylipids is emerging as an important mechanism that contributes to the complexity of proteomes. Originally considered solely as markers of oxidative insult, more recently the modifications of proteins by lipid peroxidation products are being recognized as having roles in cell signaling with relevance to redox homeostasis, adaptive response and inflammatory resolution. We report on mass spectrometry-based methods in conjunction with chemoselective strategies that are capable of detecting, identifying and characterizing protein adducts of lipoxidation products in biological samples with high complexity.
authors.affiliation: Oregon State University, Corvallis, Oregon, USA.
authors.forename: Claudia
authors.initials: C
authors.lastname: Maier