title: Neuropilin-2 genomic elements drive cre recombinase expression in primitive blood, vascular and neuronal lineages.
abstract: We have established a novel Cre mouse line, using genomic elements encompassing the Nrp2 locus, present within a bacterial artificial chromosome clone. By crossing this Cre driver line to R26R LacZ reporter mice, we have documented the temporal expression and lineage traced tissues in which Cre is expressed. Nrp2-Cre drives expression in primitive blood cells arising from the yolk sac, venous and lymphatic endothelial cells, peripheral sensory ganglia, and the lung bud. This mouse line will provide a new tool to researchers wishing to study the development of various tissues and organs in which this Cre driver is expressed, as well as allow tissue-specific knockout of genes of interest to study protein function. This work also presents the first evidence for expression of Nrp2 protein in a mesodermal progenitor with restricted hematopoietic potential, which will significantly advance the study of primitive erythropoiesis. genesis 53:709-717, 2015. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Centre for Cancer Biology and University of South Australia, Frome Road, Adelaide, South Australia, 5000, Australia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Wiszniak
authors.affiliation: Centre for Cancer Biology and University of South Australia, Frome Road, Adelaide, South Australia, 5000, Australia.
authors.forename: Michaela
authors.initials: M
authors.lastname: Scherer
authors.affiliation: Centre for Cancer Biology and University of South Australia, Frome Road, Adelaide, South Australia, 5000, Australia.
authors.forename: Hayley
authors.initials: H
authors.lastname: Ramshaw
authors.affiliation: Centre for Cancer Biology and University of South Australia, Frome Road, Adelaide, South Australia, 5000, Australia.
authors.forename: Quenten
authors.initials: Q
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D019070:Cell Lineage; D047108:Embryonic Development / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology; D004912:Erythrocytes / Q000378:metabolism; D005724:Ganglia / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D019426:Integrases / Q000096:biosynthesis*; D008822:Mice, Transgenic; D008954:Models, Biological; D009432:Neural Crest / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology; D039943:Neuropilin-2 / Q000235:genetics*; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D039943:Neuropilin-2; C045073:Cre recombinase; D019426:Integrases
keywords: Cre; Nrp2; enhancer; erythrocyte; neural crest; neuropilin; vascular