title: Potentiometric chemosensor for neopterin, a cancer biomarker, using an electrochemically synthesized molecularly imprinted polymer as the recognition unit.
abstract: With an established procedure of molecular imprinting, a synthetic polymer receptor for the neopterin cancer biomarker was devised and used as a recognition unit of a potentiometric chemosensor. For that, bis-bithiophene derivatized with cytosine and bithiophene derivatized with boronic acid were used as functional monomers. The open-circuit potential (OCP) based transduction under flow-injection analysis conditions (FIA) determined neopterin in the concentration range of 0.15-2.5mM with the 22 µM limit of detection (LOD) and 7.01(±0.15) mVmM(-1) sensitivity indicating its potential suitability in clinical analysis applications. The molecularly imprinted polymer (MIP) film showed an appreciable apparent imprinting factor of ~6. The chemosensor successfully discriminated the interferences including the 6-biopterin and pterin structural analogs of neopterin as well as glucose and creatinine. Moreover, it determined neopterin in synthetic serum samples.
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. Electronic address: psharma@ichf.edu.pl.
authors.forename: Piyush Sindhu
authors.initials: PS
authors.lastname: Sharma
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wojnarowicz
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Sosnowska
authors.affiliation: Dipartimento di Scienza e Alta Tecnologia, Università degli Studi dell'Insubria, Via Valleggio 11, 22100 Como, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Benincori
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Noworyta
authors.affiliation: Department of Chemistry, University of North Texas, Denton, 1155, Union Circle, #305070, TX 76203-5017, USA. Electronic address: Francis.Dsouza@unt.edu.
authors.forename: Francis
authors.initials: F
authors.lastname: D'Souza
authors.affiliation: Department of Physical Chemistry of Supramolecular Complexes, Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland; Faculty of Mathematics and Natural Sciences, School of Science, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego 1/3, 01-815 Warsaw, Poland. Electronic address: wkutner@ichf.edu.pl.
authors.forename: Wlodzimierz
authors.initials: W
authors.lastname: Kutner
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D003217:Conductometry / Q000295:instrumentation*; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D054802:Molecular Imprinting / Q000379:methods*; D019798:Neopterin / Q000097:blood*; D011108:Polymers / Q000737:chemistry*; D011199:Potentiometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D014408:Biomarkers, Tumor; D011108:Polymers; D019798:Neopterin
keywords: Cancer biomarker; Molecularly imprinted polymer; Neopterin; Open-circuit potential; Potentiometric chemosensor