title: Enhanced visible light photocatalytic inactivation of Escherichia coli using silver nanoparticles as photocatalyst.
abstract: The silver nanoparticles (AgNPs) were green synthesized using Cirsium arvense plant extract as a reducing and stabilizing agent, with superior photo inactivation activity against Escherichia coli (E. coli). The synthesized AgNPs had crystalline structure and were characterized by UV-vis spectroscopy, XRD, HRTEM, SEM, EDX and FT-IR. The formation of nanoparticles was observed at different pH and different plant extract concentrations and it was found that at higher pH (pH>6) and at lower concentration (10 mL), the reducing and stabilizing efficiency of plant extract was increased. The synthesized AgNPs had small size (<15 nm) and spherical shape. The AgNPs were evaluated for antibacterial activity against E. coli. Before transferring it to antibacterial activity, it was placed under visible light for 120 min. The same experiment was performed in dark as a control medium. The photo irradiated AgNPs were observed to be more effective against E. coli. The results showed, that the diameter of zone of inhibition of visible light irradiated AgNPs against E. coli was 23 (±0.5)mm and in dark was 11 (±0.4)mm.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China; Institute of Chemical Sciences, Gomal University D.I.Khan, KP, Pakistan.
authors.forename: Kamran
authors.initials: K
authors.lastname: Tahir
authors.affiliation: Institute of Chemical Sciences, Gomal University D.I.Khan, KP, Pakistan.
authors.forename: Sadia
authors.initials: S
authors.lastname: Nazir
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address: bsli@mail.buct.edu.cn.
authors.forename: Baoshan
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Arif Ullah
authors.initials: AU
authors.lastname: Khan
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Zia Ul Haq
authors.initials: ZU
authors.lastname: Khan
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmad
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Qudrat Ullah
authors.initials: QU
authors.lastname: Khan
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Yunchen
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D031180:Cirsium / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010936:Plant Extracts; D012834:Silver
keywords: Antibacterial activity; Cirsium arvense; Escherichia coli; Green synthesis; Nanoparticles