title: A gold nanocluster-based sensor for sensitive uric acid detection.
abstract: In this report, we developed a highly sensitive and selective sensor for the detection of uric acid. Gold nanoclusters were synthesized with bovine serum albumin as the template material. Under the catalysis of urate oxidase, hydrogen peroxide was generated, which quenched the fluorescence from the gold nanoclusters. Furthermore, excessive iodide was found capable of enhancing this quenching effect, which significantly improved the sensitivity of the detection. Under an optimized condition, the extent of quenching was found linearly related to uric acid concentration in the range of 0.7-80 μM, and uric acid as low as 120 nM could be detected. With simple dilutions, blood samples could be analyzed, and satisfactory recoveries were obtained.
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Xu
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Li
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Tu
authors.affiliation: The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou College of Chemistry, Chemical Engineering and Materials Science, Suzhou University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China. Electronic address: yanjl@suda.edu.cn.
authors.forename: Jilin
authors.initials: J
authors.lastname: Yan
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007454:Iodides / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis / Q000097:blood* / Q000737:chemistry
chemical_list: D007454:Iodides; D014527:Uric Acid; D006046:Gold; D006861:Hydrogen Peroxide; D014503:Urate Oxidase
keywords: Fluorescence quenching; Gold nanoclusters; Hydrogen peroxide; Iodide; Urate oxidase; Uric acid