title: Alterations of Ca(v)1.2 and 5-hydroxytryptamine in rat hearts after positional asphyxia.
abstract: We investigated alterations of cardiac Ca(v)1.2 and 5-hydroxytryptamine (5-HT) associated with positional asphyxia. Male rats were divided into five groups: a control group with no restraint, group 1 restrained for 1 h, group 2 restrained for 2 h, group 3 restrained for 4 h, and group 4 restrained for 8 h. The rats that were restrained for 8 h ultimately suffered fatal asphyxia. After the restraint periods, the rats were sacrificed and immunohistochemistry was performed to evaluate the expressions of Ca(v)1.2 and 5-HT in the heart. Sections were analyzed by digital image analysis. Cardiac expression of Ca(v)1.2 and 5-HT proteins were significantly decreased by positional asphyxia in the rat, shown by integrated optical density (IOD) compared to controls. Our findings indicate that Ca(v)1.2 and 5-HT alterations could cause abnormal cardiac function, and the proteins investigated here may be useful for investigating the mechanisms underlying positional asphyxia.
authors.affiliation: a Library of Dali University , Dali, Yunnan Province , China.
authors.forename: X-F
authors.initials: XF
authors.lastname: Li
authors.affiliation: b Department of Pathology , School of Basic Medical Sciences, Dali University , Yunnan Province , China.
authors.forename: Q-Y
authors.initials: QY
authors.lastname: Huang
mesh_terms: D000818:Animals; D001237:Asphyxia / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000009:adverse effects*; D012701:Serotonin / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D020746:Calcium Channels, L-Type; C419385:L-type calcium channel alpha(1C); D012701:Serotonin
keywords: 5-HT; 5-hydroxytryptamine; Ca (v)1.2; positional asphyxia