title: Taxonomic profiling of bacterial community structure from coastal sediment of Alang-Sosiya shipbreaking yard near Bhavnagar, India.
abstract: The Alang-Sosiya shipbreaking yard (ASSBY) is considered the largest of its kind in the world, and a major source of anthropogenic pollutants. The aim of this study was to investigate the impact of shipbreaking activities on the bacterial community structure with a combination of culture-dependent and culture-independent approaches. In the culture-dependent approach, 200 bacterial cultures were isolated and analyzed by molecular fingerprinting and 16S ribosomal RNA (r-RNA) gene sequencing, as well as being studied for degradation of polycyclic aromatic hydrocarbons (PAHs). In the culture-independent approach, operational taxonomic units (OTUs) were related to eight major phyla, of which Betaproteobacteria (especially Acidovorax) was predominantly found in the polluted sediments of ASSBY and Gammaproteobacteria in the pristine sediment sample. The statistical approaches showed a significant difference in the bacterial community structure between the pristine and polluted sediments. To the best of our knowledge, this is the first study investigating the effect of shipbreaking activity on the bacterial community structure of the coastal sediment at ASSBY.
authors.affiliation: Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India. Electronic address: patelva1986@gmail.com.
authors.forename: Vilas
authors.initials: V
authors.lastname: Patel
authors.affiliation: Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune 411 007, Maharashtra, India.
authors.forename: Hitendra
authors.initials: H
authors.lastname: Munot
authors.affiliation: Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India.
authors.forename: Varun
authors.initials: V
authors.lastname: Shah
authors.affiliation: Molecular Biology Unit, National Center for Cell Science, Ganeshkhind, Pune 411 007, Maharashtra, India.
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
authors.affiliation: Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India. Electronic address: datta_madamwar@yahoo.com.
authors.forename: Datta
authors.initials: D
authors.lastname: Madamwar
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics; D001673:Biodegradation, Environmental; D019015:Geologic Sediments / Q000382:microbiology*; D007194:India; D007221:Industry; D059013:Microbial Consortia / Q000235:genetics; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D012336:RNA, Ribosomal, 16S; D012767:Ships; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical
keywords: 16S rRNA gene; Alang–Sosiya shipbreaking yard; Bacterial diversity; Proteobacteria