title: Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes in Thai adults with chronic periodontitis.
abstract: OBJECTIVE
  To investigate the distribution of Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes among isolates from Thai chronic periodontitis patients.
  
  
  METHODS
  Forty-four adult Thai periodontitis patients were assessed by a full mouth recording for CAL, PPD, and BOP. Seventy-nine strains of A. actinomycetemcomitans were isolated from deep pockets on selective TSBV agar and 17 strains were isolated from shallow pockets. The strains were serotyped using PCR and subtyped using DGGE.
  
  
  RESULTS
  The prevalence of A. actinomycetemcomitans was 84.1%. Non-serotypeable A. actinomycetemcomitans strains occurred equally frequent as serotypeable (54.5%); serotype a 18.2%, serotype c 15.9%, serotype e 9.1%, and serotype f 11.4%. Serotype b and d were not detected. A JP2 like strain but serotyped as c was isolated from two patients, and another two strains showed an 886bp insertion on the ltx promoter of their A. actinomycetemcomitans isolates. DGGE typing disclosed 16 different subtypes among the non-serotypeable strains. Two of them (NS1 and NS2) were more common (12.7 and 10.1%) among the strains than the other 14 subtypes (˂5.1%). Most patients showed only one subtype (32.4%) but 29.7% had 2 and 3 different subtypes while 8.1% revealed 4 subtypes in one and the same deep pocket.
  
  
  CONCLUSIONS
  This study showed a greater subtype diversity of A. actinomycetemcomitans predominated by non-serotypeable strains than previously reported in an adult Thai population. It was also revealed for the first time that isolates with a 530bp deletion or 886bp insertion of the ltx promoter were serotyped as serotype c.
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand.
authors.forename: Nuntiya
authors.initials: N
authors.lastname: Pahumunto
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand.
authors.forename: Praphansri
authors.initials: P
authors.lastname: Ruangsri
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand.
authors.forename: Mutita
authors.initials: M
authors.lastname: Wongsuwanlert
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, Thailand.
authors.forename: Supatcharin
authors.initials: S
authors.lastname: Piwat
authors.affiliation: Department of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Dahlen
authors.affiliation: Common Oral Diseases and Epidemiology Research Center and the Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat Yai 90112, Thailand. Electronic address: rawee.t@psu.ac.th.
authors.forename: Rawee
authors.initials: R
authors.lastname: Teanpaisan
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification*; D044466:Asian People / Q000235:genetics; D055113:Chronic Periodontitis / Q000235:genetics / Q000382:microbiology*; D058645:Denaturing Gradient Gel Electrophoresis; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D065288:Serogroup; D012703:Serotyping
keywords: Aggregatibacter actinomycetemcomitans; Chronic periodontitis; DGGE; Leukotoxin gene; Serotype; Subtype