title: A Novel Staphylococcal Cassette Chromosome mec Type XI Primer for Detection of mecC-Harboring Methicillin-Resistant Staphylococcus aureus Directly from Screening Specimens.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) screening using real-time PCR has decreased in sensitivity due to the emergence of variant staphylococcal cassette chromosome mec element (SCCmec) types. We have designed and validated a novel SCCmec XI primer, which enables for the first time the rapid detection of mecC-harboring MRSA directly from nasopharyngeal swabs without prior cultivation.
authors.affiliation: Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Petersdorf
authors.affiliation: Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Herma
authors.affiliation: Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Rosenblatt
authors.affiliation: Robert Koch Institute, Department of Infectious Diseases, National Centre for Staphylococci and Enterococci, Wernigerode, Gemany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Layer
authors.affiliation: Institute of Medical Microbiology and Hospital Hygiene, University Clinic of Heinrich-Heine University Düsseldorf, Düsseldorf, Germany birgit.henrich@uni-duesseldorf.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Henrich
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009305:Nasopharynx / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D017931:DNA Primers