title: Three plasma metabolite signatures for diagnosing high altitude pulmonary edema.
abstract: High-altitude pulmonary edema (HAPE) is a potentially fatal condition, occurring at altitudes greater than 3,000 m and affecting rapidly ascending, non-acclimatized healthy individuals. However, the lack of biomarkers for this disease still constitutes a bottleneck in the clinical diagnosis. Here, ultra-high performance liquid chromatography coupled with Q-TOF mass spectrometry was applied to study plasma metabolite profiling from 57 HAPE and 57 control subjects. 14 differential plasma metabolites responsible for the discrimination between the two groups from discovery set (35 HAPE subjects and 35 healthy controls) were identified. Furthermore, 3 of the 14 metabolites (C8-ceramide, sphingosine and glutamine) were selected as candidate diagnostic biomarkers for HAPE using metabolic pathway impact analysis. The feasibility of using the combination of these three biomarkers for HAPE was evaluated, where the area under the receiver operating characteristic curve (AUC) was 0.981 and 0.942 in the discovery set and the validation set (22 HAPE subjects and 22 healthy controls), respectively. Taken together, these results suggested that this composite plasma metabolite signature may be used in HAPE diagnosis, especially after further investigation and verification with larger samples.
authors.affiliation: Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Guangguo
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of outpatient, No. 22 Hospital of PLA, Geermu 816000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Li
authors.affiliation: State key laboratory of Medical Genetics and school of life sciences, central south university, changsha, 430013, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Medical Teaching, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
mesh_terms: D000328:Adult; D000532:Altitude Sickness / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers; D001784:Blood Gas Analysis; D001794:Blood Pressure; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006339:Heart Rate; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood* / Q000175:diagnosis*; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D012372:ROC Curve
chemical_list: D015415:Biomarkers