title: Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells.
abstract: BACKGROUND
  Primitive endoderm is a cell lineage segregated from the epiblast in the blastocyst and gives rise to parietal and visceral endoderm. Sox7 is a member of the SoxF gene family that is specifically expressed in primitive endoderm in the late blastocyst, although its function in this cell lineage remains unclear.
  
  
  RESULTS
  Here we characterize the function of Sox7 in primitive endoderm differentiation using mouse embryonic stem (ES) cells as a model system. We show that ectopic expression of Sox7 in ES cells has a marginal effect on triggering differentiation into primitive endoderm-like cells. We also show that targeted disruption of Sox7 in ES cells does not affect differentiation into primitive endoderm cells in embryoid body formation as well as by forced expression of Gata6.
  
  
  CONCLUSIONS
  These data indicate that Sox7 function is supplementary and not essential for this differentiation from ES cells.
authors.affiliation: Laboratory for Pluripotent cell studies, RIKEN, Centre for Developmental Biology, 2-2-3, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. mk704@cam.ac.uk.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kinoshita
authors.affiliation: Laboratory for Pluripotent cell studies, RIKEN, Centre for Developmental Biology, 2-2-3, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. d56t10@hotmail.co.jp.|Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan. d56t10@hotmail.co.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shimosato
authors.affiliation: Laboratory for Pluripotent cell studies, RIKEN, Centre for Developmental Biology, 2-2-3, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. m-yamane@cdb.riken.jp.
authors.forename: Mariko
authors.initials: M
authors.lastname: Yamane
authors.affiliation: Laboratory for Pluripotent cell studies, RIKEN, Centre for Developmental Biology, 2-2-3, Minatojima-Minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. niwa@kumamoto-u.ac.jp.|Laboratory for Development and Regenerative Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunokicho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan. niwa@kumamoto-u.ac.jp.|Present address: Department of Pluripotent Stem Cell Biology, Institure of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto, 860-0811, Japan. niwa@kumamoto-u.ac.jp.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053595:Embryonic Stem Cells / Q000378:metabolism*; D004707:Endoderm / Q000166:cytology / Q000196:embryology*; D050991:GATA4 Transcription Factor / Q000378:metabolism; D050993:GATA6 Transcription Factor / Q000378:metabolism; D024002:HMGB Proteins / Q000378:metabolism; D051379:Mice; D055760:SOXF Transcription Factors / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C484171:Gata4 protein, mouse; C494760:Gata6 protein, mouse; D024002:HMGB Proteins; D055760:SOXF Transcription Factors; C511606:Sox17 protein, mouse; C102515:Sox7 protein, mouse