title: ROCK inhibitor Y27632 promotes proliferation and diminishes apoptosis of marmoset induced pluripotent stem cells by suppressing expression and activity of caspase 3.
abstract: Previous studies reported that Rho-associated kinase inhibitor Y27632 markedly diminishes human embryonic stem cell and induced pluripotent stem cell (iPSC) dissociation-induced apoptosis and increases cloning efficiency in a feeder-free culture system. However, the mechanisms by which Y27632 protects pluripotent stem cells from apoptosis remain unknown. In the present study, we tested the effects of Y27632 on single dissociated marmoset iPSCs in a feeder-free culture. The results showed that Y27632 promoted the number of cells proliferating after passage by single-cell dissociation in a dose-dependent manner. The Rho-associated kinase inhibitor Y27632 markedly increased the cloning efficiency of marmoset iPSCs without affecting their karyotype and the expression of pluripotency markers. Meanwhile, Y27632 markedly diminished apoptosis of the marmoset iPSCs under even more severe conditions by suppressing the expression and activity of caspase 3. Taken together, the present results suggest that this reagent is effective in improving the cultural system of primate iPSCs.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China; Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia, China.
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shu
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia, China.
authors.forename: Chengwen
authors.initials: C
authors.lastname: He
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia, China.
authors.forename: Yujiong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Ou
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China; Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, College of Life Science, Ningxia University, Yinchuan, Ningxia, China. Electronic address: wuyh77@zstu.edu.cn.
authors.forename: Yulong
authors.initials: Y
authors.lastname: He
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002144:Callithrix; D053148:Caspase 3 / Q000378:metabolism*; D061945:Caspase Inhibitors; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells; D004791:Enzyme Inhibitors / Q000494:pharmacology; D059785:Karyotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects*; D011725:Pyridines / Q000494:pharmacology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases; D053148:Caspase 3
keywords: Apoptosis; Caspase 3; Induced pluripotent stem cell; Marmoset; Rho-associated kinase inhibitor Y27632