title: Spin Labeling Studies of Transmembrane Signaling and Transport: Applications to Phototaxis, ABC Transporters and Symporters.
abstract: Membrane proteins still represent a major challenge for structural biologists. This chapter will focus on the application of continuous wave and pulsed EPR spectroscopy on spin-labeled membrane proteins. Site-directed spin labeling EPR spectroscopy has evolved as a powerful tool to study the structure and dynamics of proteins, especially membrane proteins, as this method works largely independently of the size and complexity of the biological system under investigation. This chapter describes applications of this technique to three different systems: the archaeal photoreceptor/-transducer complex SRII/HtrII as an example for transmembrane signaling and two transport systems, the histidine ATP-binding cassette transporter HisQMP, and the sodium-proline symporter PutP.
authors.affiliation: Physics Department, University of Osnabrück, Barbarastr. 7, Osnabrück, Germany.
authors.forename: Johann P
authors.initials: JP
authors.lastname: Klare
authors.affiliation: Physics Department, University of Osnabrück, Barbarastr. 7, Osnabrück, Germany. Electronic address: hsteinho@uni-osnabrueck.de.
authors.forename: Heinz-Jürgen
authors.initials: HJ
authors.lastname: Steinhoff
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000378:metabolism*; D026921:Amino Acid Transport Systems, Neutral / Q000737:chemistry / Q000378:metabolism*; D001105:Archaea / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D019843:Archaeal Proteins / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001692:Biological Transport; D002465:Cell Movement; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D008027:Light; D008958:Models, Molecular; D011487:Protein Conformation; D012486:Salmonella typhimurium / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D013113:Spin Labels; D027981:Symporters / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D026921:Amino Acid Transport Systems, Neutral; D019843:Archaeal Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C434451:HtrII protein, Natronobacterium pharaonis; D013113:Spin Labels; D027981:Symporters; C060609:PutP protein, E coli
keywords: EPR; Electron paramagnetic resonance spectroscopy; SDSL; Site-directed spin labeling; Transmembrane signaling; Transmembrane transport; Transport proteins