title: Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for SiRNA Delivery.
abstract: The expression of a gene can be specifically downregulated by small interfering RNA (SiRNA). Modified carbon nanotubes (CNT) can be used to protect SiRNA and facilitate its entry into cells. Regardless of that, simple and efficient functionalization of CNT is lacking. Effective SiRNA delivery can be carried out using non-covalently functionalized CNT, where non-covalent (versus covalent) functionalization is simpler and more expeditious. Non-covalently functionalized single walled carbon nanotubes (SWCNT) that include a lipopolymer are described here. Polyethylenimine (PEI) conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG) was generated and the products used to disperse CNT to form DSPE-PEG-PEI/CNT (DGI/C), an agent capable of facilitating SiRNA delivery to cells in vitro and organs and cells in vivo.
authors.affiliation: Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada.|London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada.
authors.forename: King Sun
authors.initials: KS
authors.lastname: Siu
authors.affiliation: Institute of Immunotherapy, Nanchang University and Jiangxi Academy of Medical Sciences, Nanchang, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada.|London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada.
authors.forename: Xiufen
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada.|London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Koropatnick
authors.affiliation: Department of Pathology, Surgery, and Oncology, University of Western Ontario, Windermere Road, University Hospital C8-111, London, ON, N6A 5A5, Canada. weiping.min@uwo.ca.|London Regional Cancer Program, Lawson Health Research Institute, London, ON, Canada. weiping.min@uwo.ca.|Institute of Immunotherapy, Nanchang University and Jiangxi Academy of Medical Sciences, Nanchang, China. weiping.min@uwo.ca.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Min
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D014162:Transfection
chemical_list: D004337:Drug Carriers; D037742:Nanotubes, Carbon; D010714:Phosphatidylethanolamines; D034741:RNA, Small Interfering; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
keywords: Carbon nanotube s; Gene silencing; PEG; PEI; Polyethylene glycol; Polyethylenimine; RNAi; SiRNA delivery