title: Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development.
abstract: In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.
authors.affiliation: School of Food Science and Engineering, Qilu University of Technology, Jinan 250014, China. sgjsyh@163.com.
authors.forename: Guijin
authors.initials: G
authors.lastname: Sun
authors.affiliation: Biology Institute, Shandong Academy of Sciences, Jinan 250014, China. hliukch@sdas.org.
authors.forename: Kechun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Biology Institute, Shandong Academy of Sciences, Jinan 250014, China. xuewang1978@126.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Qilu University of Technology, Jinan 250014, China. sbchen2007@163.com.
authors.forename: Xiuhe
authors.initials: X
authors.lastname: Liu
authors.affiliation: Biology Institute, Shandong Academy of Sciences, Jinan 250014, China. qiuxiahe1980@126.com.
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan. cdhsiao@cycu.edu.tw.
authors.forename: Chung-Der
authors.initials: CD
authors.lastname: Hsiao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019040:E-Selectin / Q000235:genetics / Q000276:immunology; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D047108:Embryonic Development; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D006410:Hematopoiesis / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007249:Inflammation; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D015027:Zebrafish / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D029961:Zebrafish Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D019040:E-Selectin; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D029961:Zebrafish Proteins
keywords: E-selectin; expression; identification; lipopolysaccharide; zebrafish