title: MiR-30a Inhibits the Epithelial--Mesenchymal Transition of Podocytes through Downregulation of NFATc3.
abstract: MicroRNAs (miRNAs) possess an important regulating effect among numerous renal diseases, while their functions in the process of epithelial-to-mesenchymal transition (EMT) after podocyte injury remain unclear. The purpose of our study is to identify the potential functions of miR-30a in EMT of podocytes and explore the underlying mechanisms of miR-30a in the impaired podocytes. The results revealed that downregulation of miR-30a in podocyte injury animal models and patients, highly induced the mesenchymal markers of EMT including Collagen I, Fibronectin and Snail. Furthermore, overexpression of miR-30a enhances epithelial markers (E-cadherin) but diminished mesenchymal markers (Collagen I, Fibronectin and Snail) in podocytes. In addition, we established miR-30a target NFATc3, an important transcription factor of Non-canonical Wnt signaling pathway. More importantly, our findings demonstrated that the augmentation of miR-30a level in podocytes inhibits the nuclear translocation of NFATc3 to protect cytoskeleton disorder or rearrangement. In summary, we uncovered the protective function of miR30a targeting NFATc3 in the regulation of podocyte injury response to EMT.
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. pengrui911@foxmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. shmily900519@foxmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouyuru93@sina.com.|The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouyuru93@sina.com.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The First Hospital of Xi'an, Xi'an 710002, China. zhaoya2508@sina.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lqianyin@gmail.com.
authors.forename: Qianyin
authors.initials: Q
authors.lastname: Li
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. dongshengni@outlook.com.|The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. dongshengni@outlook.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Ni
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. hyx_zuichu@outlook.com.|The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. hyx_zuichu@outlook.com.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lys960110@sina.com.|The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lys960110@sina.com.
authors.forename: Yaoshui
authors.initials: Y
authors.lastname: Long
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. keithljn@sina.cn.|The College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. keithljn@sina.cn.
authors.forename: Jianing
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhongshilyu@sina.com.
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Lyu
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhaominmao@sina.com.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Mao
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yyokyy1126@hotmail.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lyhuang0603@sina.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. zhaohui@cuhk.edu.hk.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Core Facility of Experiments Training, Chongqing Medical University, Chongqing 400016, China. geli@cqmu.edu.cn.
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.affiliation: The Divsion of Molecular Nephrology and the Creative Training Center for Undergraduates, the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouqin@cqmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D002460:Cell Line; D024042:Collagen Type I / Q000096:biosynthesis; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics / Q000502:physiology*; D005353:Fibronectins / Q000096:biosynthesis; D015972:Gene Expression Regulation, Neoplastic; D005923:Glomerulosclerosis, Focal Segmental / Q000473:pathology; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D050778:NFATC Transcription Factors / Q000096:biosynthesis* / Q000378:metabolism; D050199:Podocytes / Q000378:metabolism*; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000096:biosynthesis; D060449:Wnt Signaling Pathway / Q000502:physiology
chemical_list: D015820:Cadherins; D024042:Collagen Type I; D005353:Fibronectins; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D050778:NFATC Transcription Factors; C494008:NFATC3 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors
keywords: epithelial-to-mesenchymal transition (EMT); microRNA-30a (miR-30a); nuclear factor of activated T cells 3 (NFATc3); podocyte