title: [Effects of resveratrol on hippocampal astrocytes and expression of TNF-α in Alzheimer's disease model rate].
abstract: OBJECTIVE
  To explore the effects of resveratrol on astrocyte and TNF-α in hippocampus of Alzheimer's disease (AD) model rats.
  
  
  METHODS
  Sixty rats were randomly divided into six groups: sham control group, model group, resveratrol 20, 40, 80 mg/kg group, and estradiol valerate group (0.8 mg/kg). The model of AD was established by ovariectomy combined injection of D-galactose (100 mg/kg). Twelve weeks later, the heart perfusion in vivo was done and then the hippocampus was fixed. Additionally, the changes of hippocampal astrocytes and TNF-α expression were detected by immunohistochemistry.
  
  
  RESULTS
  The levels of glial fibrillary acidic protein (GFAP) and TNF-α in the model group were significantly higher than those of the sham control group (P < 0.01). No marked difference in the production of GFAP was observed between the resveratrol 20 mg/kg group and the model group (P > 0.05). However, the resveratrol 40, 80 mg/kg and estradiol valerate treated groups showed a decrease in the expression of GFAP compared with the model group (P < 0.01). Moreover, with the increasing of resveratrol concentration, the expression of GFAP decreased gradually. The levels of TNF-α decreased markedly in Res 20, 40, 80 mg/kg and estradiol valerate group compared with the model group (P < 0.01).
  
  
  CONCLUSIONS
  These results suggest that the activation of astrocytes and the secretion of TNF-α can be inhibited by Res in AD rats.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Cheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000473:pathology; D004195:Disease Models, Animal; D004958:Estradiol / Q000031:analogs & derivatives; D005260:Female; D005690:Galactose; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010052:Ovariectomy; D051381:Rats; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D004958:Estradiol; D000077185:Resveratrol; D005690:Galactose