title: Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch.
abstract: Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able to allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold can generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations and M2 polarized macrophages that together lead to a local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in a xenogeneic setting, but it remains unclear whether it can be therapeutic in a model of muscle diseases. We demonstrated here for the first time that orthotopic transplantation of a decellularized diaphragmatic muscle from wild animals promoted tissue functional recovery in an established atrophic mouse model. In particular, ECM supported a local immunoresponse activating a pro-regenerative environment and stimulating host muscle progenitor cell activation and migration. These results indicate that acellular scaffolds may represent a suitable regenerative medicine option for improving performance of diseased muscles.
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy. Electronic address: m.piccoli@irpcds.org.
authors.forename: M
authors.initials: M
authors.lastname: Piccoli
authors.affiliation: Stem Cells & Regenerative Medicine Section, Developmental Biology & Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital, London, United Kingdom. Electronic address: l.urbani@ucl.ac.uk.
authors.forename: L
authors.initials: L
authors.lastname: Urbani
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy.
authors.forename: M E
authors.initials: ME
authors.lastname: Alvarez-Fallas
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Franzin
authors.affiliation: Department of Cardiac, Thoracic and Vascular Sciences, University of Padua, Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Dedja
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Bertin
authors.affiliation: Department of Medicine, University of Padua, Padua, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Zuccolotto
authors.affiliation: Veneto Institute of Oncology IOV - IRCCS, Padua, Italy; Department of Surgery, Oncology and Gastroenterology, University of Padua, Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rosato
authors.affiliation: Department of Industrial Engineering, University of Padua, Padua, Italy; Centre for Mechanics of Biological Materials, University of Padua, Padua, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Pavan
authors.affiliation: Department of Industrial Engineering, University of Padua, and Venetian Institute of Molecular Medicine, Padua, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Elvassore
authors.affiliation: Stem Cells & Regenerative Medicine Section, Developmental Biology & Cancer Programme, UCL Institute of Child Health and Great Ormond Street Hospital, London, United Kingdom. Electronic address: Paolo.DeCoppi@gosh.nhs.uk.
authors.forename: P
authors.initials: P
authors.lastname: De Coppi
authors.affiliation: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy. Electronic address: m.pozzobon@irpcds.org.
authors.forename: M
authors.initials: M
authors.lastname: Pozzobon
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000502:physiology*; D005109:Extracellular Matrix; D051379:Mice; D054457:Tissue Scaffolds
keywords: Decellularization; Extracellular matrix; Regenerative medicine; Skeletal muscle