title: The aPKC/Par3/Par6 Polarity Complex and Membrane Order Are Functionally Interdependent in Epithelia During Vertebrate Organogenesis.
abstract: The differential distribution of lipids between apical and basolateral membranes is necessary for many epithelial cell functions, but how this characteristic membrane organization is integrated within the polarity network during ductal organ development is poorly understood. Here we quantified membrane order in the gut, kidney and liver ductal epithelia in zebrafish larvae at 3-11 days post fertilization (dpf) with Laurdan 2-photon microscopy. We then applied a combination of Laurdan imaging, antisense knock-down and analysis of polarity markers to understand the relationship between membrane order and apical-basal polarity. We found a reciprocal relationship between membrane order and the cell polarity network. Reducing membrane condensation by exogenously added oxysterol or depletion of cholesterol reduced apical targeting of the polarity protein, aPKC. Conversely, using morpholino knock down in zebrafish, we found that membrane order was dependent upon the Crb3 and Par3 polarity protein expression in ductal epithelia. Hence our data suggest that the biophysical property of membrane lipid packing is a regulatory element in apical basal polarity.
authors.affiliation: School of Medical Sciences, ARC Centre for Advanced Molecular Imaging and Australian Centre for NanoMedicine, The University of New South Wales, Australia.|Present address: Department of Chemistry and Medical Analysis, Faculty of Science, Al-Balqa' Applied University, Al-Salt, 19117, Jordan.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abu-Siniyeh
authors.affiliation: Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, UK.
authors.forename: Dylan M
authors.initials: DM
authors.lastname: Owen
authors.affiliation: School of Medical Sciences, ARC Centre for Advanced Molecular Imaging and Australian Centre for NanoMedicine, The University of New South Wales, Australia.
authors.forename: Carola
authors.initials: C
authors.lastname: Benzing
authors.affiliation: Brain and Mind Research Institute, Sydney Medical School and Department of Health Sciences, University of Sydney, Australia.
authors.forename: Silke
authors.initials: S
authors.lastname: Rinkwitz
authors.affiliation: Brain and Mind Research Institute, Sydney Medical School and Department of Health Sciences, University of Sydney, Australia.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Becker
authors.affiliation: Department of Immunology, Genetics, and Pathology, Uppsala University, Sweden.
authors.forename: Arindam
authors.initials: A
authors.lastname: Majumdar
authors.affiliation: School of Medical Sciences, ARC Centre for Advanced Molecular Imaging and Australian Centre for NanoMedicine, The University of New South Wales, Australia.
authors.forename: Katharina
authors.initials: K
authors.lastname: Gaus
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D004848:Epithelium / Q000196:embryology / Q000378:metabolism*; D008565:Membrane Proteins; D038081:Organogenesis; D011493:Protein Kinase C / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D029961:Zebrafish Proteins; C513143:crb3a protein, zebrafish; C486818:pard3ab protein, zebrafish; D002784:Cholesterol; D011493:Protein Kinase C
keywords: apical and basolateral membranes; epithelial cell; lipid phases; membrane organization; polarity proteins; zebrafish larvae