title: Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant.
abstract: The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. Here, we show the first proteome study of a marine fungus. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of the wild type strain and its mutant. For this purpose, an optimised protein extraction protocol was established. In total, 4759 proteins were identified. The central metabolic pathway of strain LF580 was mapped using the KEGG pathway analysis and GO annotation. Employing iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to limited nutrient availability in the wild type strain due to a strong pellet formation. This information can be applied for optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum.
authors.affiliation: Research Unit Marine Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
authors.forename: Annemarie
authors.initials: A
authors.lastname: Kramer
authors.affiliation: Centre for Clinical Proteomics, Department for Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark.
authors.forename: Hans Christian
authors.initials: HC
authors.lastname: Beck
authors.affiliation: Department for Botany and Molecular Biology, Institute of Botany, Christian-Albrechts University of Kiel, Kiel, Germany.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Centre for Clinical Proteomics, Department for Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark.
authors.forename: Lars Peter
authors.initials: LP
authors.lastname: Kristensen
authors.affiliation: Research Unit Marine Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Imhoff
authors.affiliation: Research Unit Marine Ecology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Labes
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D059001:Aquatic Organisms / Q000378:metabolism*; D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D047630:Depsipeptides / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000302:isolation & purification; D063990:Gene Ontology; D008954:Models, Biological; D009154:Mutation / Q000235:genetics*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides; D005656:Fungal Proteins; D020543:Proteome