title: Comparison of outcomes obtained in murine local lymph node assays using CBA/J or CBA/Ca mice.
abstract: CBA/J and CBA/Ca mice are the recommended strains for local lymph node assays (LLNAs). Here, we report quantitative and qualitative comparisons between both mouse strains to provide useful information for the strain selection of sensitization testing. LLNA was conducted, in accordance with Organisation for Economic Co-operation and Development Test Guideline No. 429, with CBA/J and CBA/Ca mice using five chemicals including typical contact sensitizers and non-sensitizers: 2,4-dinitrochlorobenzene (DNCB), isoeugenol, α-hexylcinnamic aldehyde (HCA), propylene glycol (PG), and hexane; then outcomes were compared based on the raw data (disintegrations per minute, DPM), stimulation index (SI) values, EC3 values and positive/negative decisions. Although a significant difference was noted between DPM values derived from each strain of mice, SI values exhibited no considerable difference. The EC3 values for DNCB in CBA/J and CBA/Ca mice were 0.04 and 0.03, those for isoeugenol were 1.4 and 0.9, and those for HCA were 7.7 and 6.0, respectively. All EC values derived from each test system were almost equivalent and were within the range of acceptance criteria of the ICCVAM performance standard for LLNA. Positive/negative outcomes for all test chemicals were consistent. In conclusion, no considerable differences were observed in the final outcomes derived from CBA/J and CBA/Ca mice in LLNA. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute (CERI), 1600, Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama, 345-0043, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Maeda
authors.affiliation: Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute (CERI), 1600, Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama, 345-0043, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Hirosaki
authors.affiliation: Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute (CERI), 1600, Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama, 345-0043, Japan.
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yakata
authors.affiliation: Chemicals Assessment and Research Center, Chemicals Evaluation and Research Institute (CERI), 1600, Shimotakano, Sugito-machi, Kitakatsushika-gun, Saitama, 345-0043, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takeyoshi
mesh_terms: D000818:Animals; D004137:Dinitrochlorobenzene / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005054:Eugenol / Q000031:analogs & derivatives / Q000633:toxicity; D005069:Evaluation Studies as Topic; D005260:Female; D006586:Hexanes / Q000633:toxicity; D023441:Local Lymph Node Assay; D008198:Lymph Nodes / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D019946:Propylene Glycol / Q000633:toxicity; D018675:Toxicity Tests; D016896:Treatment Outcome
chemical_list: D004137:Dinitrochlorobenzene; D006586:Hexanes; D005054:Eugenol; C036643:isoeugenol; D019946:Propylene Glycol
keywords: CBA/Ca; CBA/J; LLNA; OECD TG429; skin sensitization