title: Characterization and Comparison of Canine Multipotent Stromal Cells Derived from Liver and Bone Marrow.
abstract: Liver-derived multipotent stromal cells (L-MSCs) may prove preferable for treatment strategies of liver diseases, in comparison to the widely studied bone marrow-derived MSCs (BM-MSCs). Canines are a large animal model, in which the pathologies of liver diseases are similar to man. This study further promotes the implementation of canine models in MSC-based treatments of liver diseases. L-MSCs were characterized and compared to BM-MSCs from the same individual. Both cell types demonstrated a spindle-shaped fibroblast-like morphology, possessed the same growth potential, and demonstrated similar immunomodulation gene expression of CD274, PTGS-1, and PTGS-2. Marked differences in cell surface markers, CD105 and CD146, distinguished these two cell populations, and L-MSCs retained a liver-specific imprinting, observed by expression of CK18 and CK19. Finally, both populations differentiated toward the osteogenic and adipogenic lineage; however, L-MSCs failed to differentiate into the chondrogenic lineage. In conclusion, characterization of canine L-MSCs and BM-MSCs demonstrated that the two cell type populations are highly comparable. Although it is still unclear which cell source is preferred for clinical application in liver treatment strategies, this study provides a foundation for future controlled studies with MSC therapy in various liver diseases in dogs before their application in man.
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .|2 Department of Visceral and Transplantation Surgery, Swiss Hepato-Pancreato-Biliary Center, University Hospital , Zurich, Switzerland .
authors.forename: Ermanno
authors.initials: E
authors.lastname: Malagola
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Michelle
authors.initials: M
authors.lastname: Teunissen
authors.affiliation: 3 Department of Surgery, Erasmus MC-University Medical Center , Rotterdam, the Netherlands .
authors.forename: Luc J W
authors.initials: LJ
authors.lastname: van der Laan
authors.affiliation: 3 Department of Surgery, Erasmus MC-University Medical Center , Rotterdam, the Netherlands .
authors.forename: Monique M A
authors.initials: MM
authors.lastname: Verstegen
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Baukje A
authors.initials: BA
authors.lastname: Schotanus
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Frank G
authors.initials: FG
authors.lastname: van Steenbeek
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Louis C
authors.initials: LC
authors.lastname: Penning
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Monique E
authors.initials: ME
authors.lastname: van Wolferen
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Marianna A
authors.initials: MA
authors.lastname: Tryfonidou
authors.affiliation: 1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University , Utrecht, the Netherlands .
authors.forename: Bart
authors.initials: B
authors.lastname: Spee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D002478:Cells, Cultured; D004285:Dogs; D008099:Liver / Q000378:metabolism*; D039902:Multipotent Stem Cells / Q000166:cytology*