title: Screening and characterization of early diagnostic antigens in excretory-secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics.
abstract: The excretory-secretory (ES) antigens from Trichinella spiralis muscle larvae are the most commonly used diagnostic antigens for trichinellosis, but specific IgG antibodies were not detected in early stage of infection. The aim of this study was to identify early diagnostic antigens from ES proteins of intestinal infective larvae (IIL), the first invasive stage of T. spiralis. Six bands (92, 52, 45, 35, 32, and 29 kDa) of IIL ES proteins were recognized by infection sera in Western blotting as early as 10 days post infection. Total of 54 T. spiralis proteins in six bands were identified by shotgun LC-MS/MS, 30 proteins were annotated, and 27 had hydrolase activity. Several proteins (serine protease, putative trypsin, deoxyribonuclease II family protein, etc.) could be considered as the potential early diagnostic antigens for trichinellosis. Our study provides new insights for screening early diagnostic antigens from intestinal worms of T. spiralis.
authors.forename: Ruo Dan
authors.initials: RD
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.forename: Jiang Yang
authors.initials: JY
authors.lastname: Duan
authors.forename: Li Ang
authors.initials: LA
authors.lastname: Wang
authors.forename: Jie Feng
authors.initials: JF
authors.lastname: Li
authors.forename: Chun Ying
authors.initials: CY
authors.lastname: Liu
authors.forename: Ge Ge
authors.initials: GG
authors.lastname: Sun
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism; D007814:Larva / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000378:metabolism / Q000469:parasitology; D040901:Proteomics; D057057:Serine Proteases / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D057057:Serine Proteases