title: Identification of two isoforms of CYP4 in Marsupenaeus japonicus and their mRNA expression profile response to benzo[a]pyrene.
abstract: CYP4 enzymes are essential components of cellular detoxification systems and play important roles in monitoring persistent organic pollutants in marine environments. However, there are few studies on CYP4 in shrimp. In this study, two CYP4 isoforms, CYP4V28 and CYP4V29, were cloned from Marsupenaeus japonicus for the first time, and the tissue distributions and mRNA expression profile in response to benzo[a]pyrene (B[a]P) were analyzed by quantitative real-time PCR (QRT-PCR). The full lengths of CYP4V28 and CYP4V29 were 1771 bp and 1647 bp respectively, with deduced amino acid sequences of 511 and 515 amino acids. The two CYP4s were predominantly expressed in the hepatopancreas and weakly expressed in other six tested tissues. As demonstrated by QRT-PCR, the mRNA levels of the two CYP4s show both a time- and dose-dependent response to B[a]P. The mRNA expression levels of CYP4V28 and CYP4V29 peaked at 12 h and 6 h respectively, and the peak level exhibited a tendency of positive correlation with the concentration of B[a]P. This study provides clues for further elucidating the function and regulation mechanisms of the two CYP4s in M. japonicas and evaluating of the biomarker potential of the two CYP4 isoforms.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Jinbin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China. Electronic address: maoyong@xmu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Mao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Zhuangzhuang
authors.initials: Z
authors.lastname: Shi
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring; D009928:Organ Specificity; D033561:Penaeidae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D060829:Arthropod Proteins; D015415:Biomarkers; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene
keywords: Benzo[a]pyrene; Biomarker; CYP4; Detoxification; Marsupenaeus japonicus; PAHs