title: Membrane Feeding Assay to Determine the Infectiousness of Plasmodium vivax Gametocytes.
abstract: The evaluation of Plasmodium vivax gametocyte infectiousness by the membrane feeding assay is herein described. While P. vivax cannot be cultured and different parasite isolates may infect mosquitoes at different rates, the protocol described in this chapter identifies critical parameters to be considered when performing the assay such as methods for the preparation of the mosquitoes, the size of the blood cup, and the blood volume used. In previous studies the data have shown that the membrane feeding assay is useful for studies of parasite biology, and the effects of transmission blocking drugs and vaccines.
authors.affiliation: Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. jetsumnon.pra@mahidol.edu.
authors.forename: Jetsumon
authors.initials: J
authors.lastname: Sattabongkot
authors.affiliation: Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Chalermpon
authors.initials: C
authors.lastname: Kumpitak
authors.affiliation: Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Kirakorn
authors.initials: K
authors.lastname: Kiattibutr
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000469:parasitology*; D009033:Culicidae / Q000469:parasitology; D005854:Germ Cells / Q000378:metabolism; D006801:Humans; D016780:Malaria, Vivax / Q000378:metabolism / Q000469:parasitology* / Q000635:transmission; D008967:Molecular Biology / Q000379:methods*; D010966:Plasmodium vivax / Q000378:metabolism / Q000472:pathogenicity*
keywords: Direct skin feed assay (DFA); Gametocyte infectiousness; Membrane feeding assay (MFA); Plasmodium vivax; Transmission blocking drugs; Transmission blocking vaccines