title: Role of Protein Kinase Akt Activation in Protective Effect of Ganglioside GM1 on PC12 Cells Exposed to H2O2.
abstract: Micro- and nanomolar concentrations of ganglioside GM1 improved viability of neuronal PC12 cells under conditions of oxidative stress and reduced H2O2-induced ROS accumulation in these cells. These effects were more pronounced at micromolar concentrations. GM1 in concentrations of 100 nM and 10 μM significantly and substantially increased basal activity of protein kinase B (Akt) (the level of phosphorylated Akt form), but had virtually no effect on its expression in PC12 cells. In the presence of PI3K inhibitor LY294002 preventing protein kinase Akt activation, the protective effect of GM1 significantly decreased. These findings suggest that activation of protein kinase Akt by GM1 contributes to improvement of PC12 cell viability by this ganglioside.
authors.affiliation: Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: I O
authors.initials: IO
authors.lastname: Zakharova
authors.affiliation: Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Sokolova
authors.affiliation: Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Akhmetshina
authors.affiliation: Laboratory of Comparative Chemistry, I. M. Sechenov Institute of Evolutional Physiology and Biochemistry of Russian Academy of Sciences, St. Petersburg, Russia. avrova@iephb.ru.
authors.forename: N F
authors.initials: NF
authors.lastname: Avrova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002867:Chromones / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D016716:PC12 Cells; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000819:agonists* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002867:Chromones; D005677:G(M1) Ganglioside; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D009025:Morpholines; D018696:Neuroprotective Agents; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D017382:Reactive Oxygen Species; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
keywords: PC12 cells; formation of reactive oxygen species; ganglioside GM1; protective effect; protein kinase B (Akt)