title: A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architecture.
abstract: Chromatin architecture determines transcriptional accessibility to DNA and consequently gene expression levels in response to developmental and environmental stimuli. Recently, chromatin remodelers such as SWI/SNF complexes have been recognized as key regulators of chromatin architecture. To gain insight into the function of these complexes during root development, we have analyzed Arabidopsis knock-down lines for one sub-unit of SWI/SNF complexes: BAF60. Here, we show that BAF60 is a positive regulator of root development and cell cycle progression in the root meristem via its ability to down-regulate cytokinin production. By opposing both the deposition of active histone marks and the formation of a chromatin regulatory loop, BAF60 negatively regulates two crucial target genes for cytokinin biosynthesis (IPT3 and IPT7) and one cell cycle inhibitor (KRP7). Our results demonstrate that SWI/SNF complexes containing BAF60 are key factors governing the equilibrium between formation and dissociation of a chromatin loop controlling phytohormone production and cell cycle progression.
authors.affiliation: Institut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, France.
authors.forename: Teddy
authors.initials: T
authors.lastname: Jégu
authors.affiliation: Institut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Domenichini
authors.affiliation: Institut des Sciences du Végétal, UPR2355 CNRS, Saclay Plant Sciences, Gif-sur-Yvette, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Blein
authors.affiliation: Institut des Sciences du Végétal, UPR2355 CNRS, Saclay Plant Sciences, Gif-sur-Yvette, France.
authors.forename: Federico
authors.initials: F
authors.lastname: Ariel
authors.affiliation: Institut des Sciences du Végétal, UPR2355 CNRS, Saclay Plant Sciences, Gif-sur-Yvette, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Christ
authors.affiliation: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology KAUST, Thuwal, Saudi Arabia.
authors.forename: Soon-Kap
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Institut des Sciences du Végétal, UPR2355 CNRS, Saclay Plant Sciences, Gif-sur-Yvette, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Crespi
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA/AgroParisTech, Versailles, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Boutet-Mercey
authors.affiliation: Institut Jean-Pierre Bourgin, UMR1318 INRA/AgroParisTech, Versailles, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Mouille
authors.affiliation: Pôle de Biologie Cellulaire, Imagif, Centre de Recherche de Gif, CNRS, IFR87, Gif-sur-Yvette, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Bourge
authors.affiliation: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology KAUST, Thuwal, Saudi Arabia.
authors.forename: Heribert
authors.initials: H
authors.lastname: Hirt
authors.affiliation: Institut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Bergounioux
authors.affiliation: Institut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Raynaud
authors.affiliation: Institut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, France; Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology KAUST, Thuwal, Saudi Arabia.
authors.forename: Moussa
authors.initials: M
authors.lastname: Benhamed
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003583:Cytokinins / Q000096:biosynthesis*; D018744:DNA, Plant / Q000235:genetics; D044127:Epigenesis, Genetic; D056426:Genetic Loci / Q000235:genetics; D006657:Histones / Q000378:metabolism; D018519:Meristem / Q000254:growth & development
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D003583:Cytokinins; D018744:DNA, Plant; D006657:Histones; C587774:KRP7 protein, Arabidopsis; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase