title: A General Method for Measuring Persister Levels in Escherichia coli Cultures.
abstract: Genetically homogeneous bacterial cultures contain persisters, cells that are not killed by bactericidal antibiotics. These cells are suggested to be involved in the establishment of chronic infections. Persister levels depend on growth conditions. Here, we discuss the parameters that have to be considered when measuring persister levels and provide a sample protocol to do it.
authors.affiliation: Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.
authors.forename: Niilo
authors.initials: N
authors.lastname: Kaldalu
authors.affiliation: Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.
authors.forename: Arvi
authors.initials: A
authors.lastname: Jõers
authors.affiliation: Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia.
authors.forename: Henri
authors.initials: H
authors.lastname: Ingelman
authors.affiliation: Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia. Tanel.Tenson@ut.ee.
authors.forename: Tanel
authors.initials: T
authors.lastname: Tenson
mesh_terms: D000667:Ampicillin / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018929:Cell Culture Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D024841:Fluoroquinolones / Q000627:therapeutic use; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000667:Ampicillin
keywords: Ampicillin; Antibiotic; Antibiotic tolerance; Fluoroquinolones; Persister