title: Impact of buserelin acetate or hCG administration on day 5 post-ovulation on subsequent luteal profile and conception rate in Murrah buffalo (Bubalus bubalis).
abstract: The present study aimed to establish the impact of buserelin acetate or hCG administration on day 5 post-ovulation on subsequent luteal profile and conception rate in buffalo. The buffalo (n=45) were subjected to an estrous synchronization protocol (synthetic analog of PGF2α administered, through intramuscular route, 11 days apart), followed by artificial insemination (AI) during mid to late estrus. On day 5 post-ovulation, buffalo were administered (i.m.) normal saline (Control, n=14), buserelin acetate (20μg, d5-BA, n=14) or human chorionic gonadotropin (3000IU, d5-hCG, n=17). Ovarian ultrasonography was conducted on the day of induced estrus and on days 0, 5, 12, 16 and 21 post-ovulation to assess preovulatory follicle or corpus luteum (CL) diameter. Also, on these days, jugular vein blood sampling was conducted for the estimation of plasma progesterone. First service conception rate was greater (χ(2)=5.18, P>0.05) in d5-BA and d5-hCG groups (71.4% and 47.1%, respectively) as compared to control (28.6%). Both treatment groups had a greater (P<0.05) CL diameter and plasma progesterone during the post-treatment period in comparison to that control treatment group. Treatment-induced accessory CL formation was observed in 92.9% and 76.5% buffalo of d5-BA and d5-hCG groups, respectively. In conclusion, buserelin acetate and hCG administration on day 5 post-ovulation leads to accessory CL formation that may have a role in enhancing conception rate.
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India. Electronic address: dranandpandey@gmail.com.
authors.forename: A K
authors.initials: AK
authors.lastname: Pandey
authors.affiliation: Department of Veterinary and Animal Husbandry Extension Education, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India.
authors.forename: G S
authors.initials: GS
authors.lastname: Dhaliwal
authors.affiliation: Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India.
authors.forename: S P S
authors.initials: SP
authors.lastname: Ghuman
authors.affiliation: Central Institute for Research on Goat, Makhdoom, Farah, Mathura 281122 Uttar Pradesh, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D002020:Buffaloes; D002064:Buserelin / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000187:drug effects* / Q000502:physiology; D004973:Estrus Synchronization; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000000981:diagnostic imaging / Q000502:physiology; D011374:Progesterone / Q000097:blood; D014463:Ultrasonography
chemical_list: D006063:Chorionic Gonadotropin; D011374:Progesterone; D002064:Buserelin
keywords: Buffalo; Corpus luteum; Gonadotropins; Preovulatory follicle; Progesterone