title: Metabolomic profiling of permethrin-treated Drosophila melanogaster identifies a role for tryptophan catabolism in insecticide survival.
abstract: Insecticides and associated synergists are rapidly losing efficacy in target insect pest populations making the discovery of alternatives a priority. To discover novel targets for permethrin synergists, metabolomics was performed on permethrin-treated Drosophila melanogaster. Changes were observed in several metabolic pathways including those for amino acids, glycogen, glycolysis, energy, nitrogen, NAD(+), purine, pyrimidine, lipids and carnitine. Markers for acidosis, ammonia stress, oxidative stress and detoxification responses were also observed. Many of these changes had not been previously characterized after permethrin exposure. From the altered pathways, tryptophan catabolism was selected for further investigation. The knockdown of some tryptophan catabolism genes (vermilion, cinnabar and CG6950) in the whole fly and in specific tissues including fat body, midgut and Malpighian tubules using targeted RNAi resulted in altered survival phenotypes against acute topical permethrin exposure. The knockdown of vermilion, cinnabar and CG6950 in the whole fly also altered survival phenotypes against chronic oral permethrin, fenvalerate, DDT, chlorpyriphos and hydramethylnon exposure. Thus tryptophan catabolism has a previously uncharacterized role in defence against insecticides, and shows that metabolomics is a powerful tool for target identification in pesticide research.
authors.affiliation: Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Brinzer
authors.affiliation: Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
authors.forename: Louise
authors.initials: L
authors.lastname: Henderson
authors.affiliation: Zoetis Inc., Kalamazoo, MI, USA.
authors.forename: Alan A
authors.initials: AA
authors.lastname: Marchiondo
authors.affiliation: Zoetis Inc., Kalamazoo, MI, USA.
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Woods
authors.affiliation: Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
authors.forename: Shireen A
authors.initials: SA
authors.lastname: Davies
authors.affiliation: Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK. Electronic address: Julian.dow@glasgow.ac.uk.
authors.forename: Julian A T
authors.initials: JA
authors.lastname: Dow
mesh_terms: D000818:Animals; D004390:Chlorpyrifos; D003634:DDT; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D007305:Insecticide Resistance; D007306:Insecticides; D055442:Metabolome; D009570:Nitriles; D026023:Permethrin; D011722:Pyrethrins; D011744:Pyrimidinones; D034622:RNA Interference; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism*
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D011744:Pyrimidinones; D026023:Permethrin; D014364:Tryptophan; D003634:DDT; C041642:hydramethylnon; D004390:Chlorpyrifos; C017690:fenvalerate
keywords: Drosophila melanogaster; Insecticide; Metabolomics; Permethrin; RNAi; Tryptophan catabolism