title: Photochemically modified diamond-like carbon surfaces for neural interfaces.
abstract: Diamond-like carbon (DLC) was modified using a UV functionalization method to introduce surface-bound amine and aldehyde groups. The functionalization process rendered the DLC more hydrophilic and significantly increased the viability of neurons seeded to the surface. The amine functionalized DLC promoted adhesion of neurons and fostered neurite outgrowth to a degree indistinguishable from positive control substrates (glass coated with poly-L-lysine). The aldehyde-functionalized surfaces performed comparably to the amine functionalized surfaces and both additionally supported the adhesion and growth of primary rat Schwann cells. DLC has many properties that are desirable in biomaterials. With the UV functionalization method demonstrated here it may be possible to harness these properties for the development of implantable devices to interface with the nervous system.
authors.affiliation: Department of Materials Science and Engineering, Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Hopper
authors.affiliation: Department of Materials Science and Engineering, Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Dugan
authors.affiliation: Department of Materials Science and Engineering, Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Gill
authors.affiliation: School of Chemistry, University of Bristol, Bristol BS8 1TS, UK.
authors.forename: E M
authors.initials: EM
authors.lastname: Regan
authors.affiliation: Department of Materials Science and Engineering, Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
authors.forename: J W
authors.initials: JW
authors.lastname: Haycock
authors.affiliation: New Jersey Neuroscience Institute at JFK Medical Center, 65 James Street, Edison, NJ, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kelly
authors.affiliation: School of Chemistry, University of Bristol, Bristol BS8 1TS, UK.
authors.forename: P W
authors.initials: PW
authors.lastname: May
authors.affiliation: Department of Materials Science and Engineering, Kroto Research Institute, The University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK. Electronic address: f.claeyssens@sheffield.ac.uk.
authors.forename: F
authors.initials: F
authors.lastname: Claeyssens
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000588:Amines / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018130:Diamond / Q000737:chemistry* / Q000633:toxicity; D008297:Male; D051379:Mice; D058117:Neural Prostheses; D055668:Photochemical Processes; D011474:Prosthesis Design; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000187:drug effects*
chemical_list: D000447:Aldehydes; D000588:Amines; D001672:Biocompatible Materials; D018130:Diamond
keywords: Aldehyde; Amine; Diamond-like carbon; Neurons; Schwann cells