title: Proteomics to Understand the Degenerative Matter.
abstract: No matter what we do, eat or practice - sooner or later we all will start loosing our abilities to control our bodily functions, maintain muscle control and innervations. Most of us will grow old and degrade by natural causes, while some of us unfortunately will be affected by fast or slow progressive diseases or even have accidents with traumas that will initiate dramatic degenerative effects. We have recently demonstrated that a shotgun proteomic approach coupled with relative quantitative dimethyl labeling (DML) method can analyze the global changes in the human brain and skeletal muscle proteome. Very limited amount of material (biopsy) is needed for comparison. The underlying objective of our studies are to identify potential protein biomarkers related to the development of degeneration but also to be able to get in detail structural characteristics of protein modifications that are initiated by disease. These proteins are involved in several biological processes including fundamental brain function, muscle development and contraction, metabolic process, enzyme activity, glycolysis, regulation of apoptosis and transport activity. We have identified a number of different factors behind this phenomenon that also reveals new knowledge about the natural aging.
authors.affiliation: BMC and SciLife Lab, Uppsala University (Department of Chemistry), Analytical Chemistry, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist