title: Measuring levels of biogenic amines and their metabolites in rat brain tissue using high-performance liquid chromatography with photodiode array detection.
abstract: We developed a method to detect biogenic amines and their metabolites in rat brain tissue using simultaneous high-performance liquid chromatography and a photodiode array detection. Measurements were made using a Hypersil Gold C-18 column (250 × 2.1 mm, 5 µm). The mobile phase was 5 mM perchloric acid containing 5 % acetonitrile. The correlation coefficient was 0.9995-0.9999. LODs (S/N = 3) and LOQs (S/N = 10) were as follows: dopamine 0.4 and 1.3 pg, 3, 4-dihydroxyphenylacetic acid 8.4 and 28.0 pg, serotonin 0.4 and 1.3 pg, 5-hydroxyindolacetic acid 3.4 and 11.3 pg, and homovanillic acid 8.4 and 28.0 pg. This method does not require derivatization steps, and is more sensitive than the widely used HPLC-UV method.
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, South Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Gu
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, South Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Jeon
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, South Korea.
authors.forename: Myung Sook
authors.initials: MS
authors.lastname: Oh
authors.affiliation: Department of Oriental Pharmaceutical Sciences, Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130-701, South Korea. seonhong@khu.ac.kr.
authors.forename: Seon-Pyo
authors.initials: SP
authors.lastname: Hong
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000032:analysis* / Q000378:metabolism*
chemical_list: D001679:Biogenic Amines; D012701:Serotonin; D004298:Dopamine
keywords: Brain tissue; Direct method; Dopamine; HPLC–PDA; Serotonin; Simultaneous determination