title: Abalone Protein Hydrolysates: Preparation, Angiotensin I Converting Enzyme Inhibition and Cellular Antioxidant Activity.
abstract: Abalone protein was hydrolyzed by enzymatic hydrolysis and the optimal enzyme/substrate (E/S) ratios were determined. Abalone protein hydrolysates (APH) produced by Protamex at E/S ratio of 1:100 showed angiotensin I converting enzyme inhibitory activity with IC50 of 0.46 mg/mL, and APH obtained by Flavourzyme at E/S ratio of 1:100 possessed the oxygen radical absorbance capacity value of 457.6 μM trolox equivalent/mg sample. Flavourzyme abalone protein hydrolysates (FAPH) also exhibited H2O2 scavenging activity with IC50 of 0.48 mg/mL and Fe(2+) chelating activity with IC50 of 2.26 mg/mL as well as high reducing power. FAPH significantly (P<0.05) protected H2O2-induced hepatic cell damage in cultured hepatocytes, and the cell viability was restored to 90.27% in the presence of FAPH. FAPH exhibited 46.20% intracellular ROS scavenging activity and 57.89% lipid peroxidation inhibition activity in cultured hepatocytes. Overall, APH may be useful as an ingredient for functional foods.
authors.affiliation: School of Food Technology and Nutrition, Chonnam National University, Jeonnam 59626, Korea.
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Marine-Bio Convergence Science, Pukyong National University, Busan 48513, Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
authors.affiliation: Korea Abalone Laboratory, Jeonnam 58275, Korea.
authors.forename: Joung-Youl
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: School of Food Technology and Nutrition, Chonnam National University, Jeonnam 59626, Korea ; Division of Food and Nutrition, Chonnam National University, Gwangju 61186, Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
keywords: abalone; angiotensin I converting enzyme; antioxidant; enzymatic hydrolysis