title: Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum.
abstract: A rational design of squaraine dyes with lipophilic and zwitterionic groups tunes cell entry, allowing for selective far-red/near-infrared imaging of plasma membrane vs. endoplasmic reticulum. They exhibit up to 110-fold fluorescence enhancement in biomembranes and enable cellular imaging at 1 nM concentration, which make them the brightest membrane probes to date.
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401, Illkirch, France. andrey.klymchenko@unistra.fr mayeul.collot@unistra.fr.
authors.forename: Mayeul
authors.initials: M
authors.lastname: Collot
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401, Illkirch, France. andrey.klymchenko@unistra.fr mayeul.collot@unistra.fr.
authors.forename: Rémy
authors.initials: R
authors.lastname: Kreder
authors.affiliation: The State Scientific Institution "Institute for Single Crystals" of the National Academy of Sciences of Ukraine, 60, Lenin Ave., Kharkiv, 61001, Ukraine.
authors.forename: Anatoliy L
authors.initials: AL
authors.lastname: Tatarets
authors.affiliation: The State Scientific Institution "Institute for Single Crystals" of the National Academy of Sciences of Ukraine, 60, Lenin Ave., Kharkiv, 61001, Ukraine.
authors.forename: Leonid D
authors.initials: LD
authors.lastname: Patsenker
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401, Illkirch, France. andrey.klymchenko@unistra.fr mayeul.collot@unistra.fr.
authors.forename: Yves
authors.initials: Y
authors.lastname: Mely
authors.affiliation: Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401, Illkirch, France. andrey.klymchenko@unistra.fr mayeul.collot@unistra.fr.
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Klymchenko
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D003503:Cyclobutanes / Q000138:chemical synthesis / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D010636:Phenols / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D003503:Cyclobutanes; D005456:Fluorescent Dyes; D010636:Phenols; C480596:squaraine