title: Dual emission fluorescent silver nanoclusters for sensitive detection of the biological coenzyme NAD+/NADH.
abstract: Fluorescent silver nanoclusters (Ag NCs) displaying dual-excitation and dual-emission properties have been developed for the specific detection of NAD(+) (nicotinamide adenine dinucleotide, oxidized form). With the increase of NAD(+) concentrations, the longer wavelength emission (with the peak at 550 nm) was gradually quenched due to the strong interactions between the NAD(+) and Ag NCs, whereas the shorter wavelength emission (peaking at 395 nm) was linearly enhanced. More important, the dual-emission intensity ratio (I395/I550), fitting by a single-exponential decay function, can efficiently detect various NAD(+) levels from 100 to 4000 μM, as well as label NAD(+)/NADH (reduced form of NAD) ratios in the range of 1-50.
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Kehan
authors.initials: K
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Mengfang
authors.initials: M
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Sanjun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China. Electronic address: hfpan@phy.ecnu.edu.cn.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Pan
authors.affiliation: Tongji Hospital Affiliated to Tongji University, Shanghai, 200065, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai, 200062, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
mesh_terms: D003067:Coenzymes / Q000032:analysis*; D057075:Enzyme Assays / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009243:NAD / Q000032:analysis*; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D003067:Coenzymes; D009243:NAD; D012834:Silver
keywords: Biological coenzyme NAD(+); Dual-emission fluorescent silver nanoclusters; Dual-emission intensity ratio; NAD(+)/NADH ratios