title: Horseradish Peroxidase-Mediated, Iodide-Catalyzed Cascade Reaction for Plasmonic Immunoassays.
abstract: This report outlines an enzymatic cascade reaction for signal transduction and amplification for plasmonic immunoassays by using horseradish peroxidase (HRP)-mediated aggregation of gold nanoparticles (AuNPs). HRP-catalyzed oxidation of iodide and iodide-catalyzed oxidation of cysteine is employed to modulate the plasmonic signals of AuNPs. It agrees well with the current immunoassay platforms and allows naked-eye readout with enhanced sensitivity, which holds great promise for applications in resource-constrained settings.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology , Beijing 100190, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Yunlei
authors.initials: Y
authors.lastname: Xianyu
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D006046:Gold / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D007454:Iodides / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D007454:Iodides; D006046:Gold; D006735:Horseradish Peroxidase