title: Marking Drosophila suzukii (Diptera: Drosophilidae) With Rubidium or 15N.
abstract: Drosophila suzukii Matsumura (Diptera: Drosophilidae) has caused significant economic damage to berry and stone fruit production regions. Markers that are systemic in plants and easily transferred to target organisms are needed to track D. suzukii exploitation of host resources and trophic interactions. High and low concentrations of the trace element, rubidium (Rb), and the stable isotope, 15N, were tested to mark D. suzukii larvae feeding on fruits of enriched strawberry plants grown in containers under greenhouse conditions. Fly marker content and proportion of flies marked 1, 7, and 14 d after emergence from enriched fruits and fly dry mass were analyzed. Nearly 100% of the flies analyzed 14 d after emerging from 15N-enriched plants were marked, whereas only 30-75% and 0-3% were marked 14 d after emerging from high and low Rb concentration plants, respectively. Rapid Rb decay, strong 15N persistence, and the economics of using these markers in the field to elucidate D. suzukii pest ecology are discussed.
authors.affiliation: Department of Horticulture, Oregon State University, 4017 Ag. and Life Sciences Bldg., Corvallis, OR 97331. jimmyklick@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Klick
authors.affiliation: Department of Horticulture, North Willamette Research and Extension Center, Oregon State University, 15210 NE Miley Rd., Aurora, OR 97002.
authors.forename: W Q
authors.initials: WQ
authors.lastname: Yang
authors.affiliation: USDA-ARS, Horticultural Crops Research Unit, 3420 NW Orchard Ave., Corvallis, OR 97330. Current address: DuPont Pioneer, 7300 NW 62nd Ave., PO Box 1004, Johnston, IA 50131.
authors.forename: D J
authors.initials: DJ
authors.lastname: Bruck
mesh_terms: D000818:Animals; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D020387:Food Chain; D031985:Fragaria / Q000502:physiology*; D007300:Insect Control / Q000379:methods*; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism*
chemical_list: D009587:Nitrogen Isotopes; D012413:Rubidium; D009584:Nitrogen
keywords: 15N; insect marking; rubidium; spotted wing drosophila; strawberry