title: Protein and bacterial interactions with nanostructured polymer coatings.
abstract: Adsorption of proteins and adhesion of bacteria to a surface is affected by chemical and physical interactions. In this study, polymer coatings and their ability to adsorb avidin and Staphylococcus aureus were investigated. The surface chemistry and topography of the polymer coatings was modified by changing the weight ratio of the hydrophobic polystyrene (PS) and the hydrophilic acrylonitrile butadiene styrene (ABS) components in the polymer blend. Avidin adsorbed less to the ABS phase compared with the PS phase. The side-on orientation of avidin on the ABS surface, however, resulted in a higher specific binding of biotinylated bovine serum albumin. Steric effects and hydrophobic protein-surface interactions decreased the activity of avidin on the PS phase. The increased hydrophobicity and roughness of the polymer coatings enhanced the adhesion of S. aureus. The avidin-coated latex surface with 55% relative surface coverage of the PS phase showed anti-microbial behavior.
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland. Electronic address: helka.juvonen@abo.fi.
authors.forename: Helka
authors.initials: H
authors.lastname: Juvonen
authors.affiliation: Faculty of Science and Engineering, Pharmaceutical Sciences Laboratory, Åbo Akademi University, Tykistökatu 6A, FI-20520 Turku, Finland.
authors.forename: Terhi
authors.initials: T
authors.lastname: Oja
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Anni
authors.initials: A
authors.lastname: Määttänen
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Jawad
authors.initials: J
authors.lastname: Sarfraz
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Emil
authors.initials: E
authors.lastname: Rosqvist
authors.affiliation: BioMediTech, Molecular Biotechnology, FI-33014, University of Tampere, Finland.
authors.forename: Tiina A
authors.initials: TA
authors.lastname: Riihimäki
authors.affiliation: Centre for Functional Materials, Laboratory of Paper Coating and Converting, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Martti
authors.initials: M
authors.lastname: Toivakka
authors.affiliation: BioMediTech, Molecular Biotechnology, FI-33014, University of Tampere, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Kulomaa
authors.affiliation: Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Pharmaceutical Biology, FI-00014, University of Helsinki, Finland.
authors.forename: Pia
authors.initials: P
authors.lastname: Vuorela
authors.affiliation: Faculty of Science and Engineering, Pharmaceutical Sciences Laboratory, Åbo Akademi University, Tykistökatu 6A, FI-20520 Turku, Finland; Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Pharmaceutical Biology, FI-00014, University of Helsinki, Finland.
authors.forename: Adyary
authors.initials: A
authors.lastname: Fallarero
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Jouko
authors.initials: J
authors.lastname: Peltonen
authors.affiliation: Centre for Functional Materials, Laboratory of Physical Chemistry, Åbo Akademi University, Porthaninkatu 3, FI-20500 Turku, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Ihalainen
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry*; D049329:Nanostructures; D011108:Polymers / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D011108:Polymers
keywords: Adhesion; Adsorption; Avidin; Polymer coating; Staphylococcus aureus; Surface modification