title: Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar.
abstract: Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity (r⩾0.9994) and afforded low limits of detection ranging from 1.55 to 10.17μgL(-1) for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% (n=6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.
authors.affiliation: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China.
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Ni
authors.affiliation: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China.
authors.forename: Binhe
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. Electronic address: wnn8511@163.com.
authors.forename: Nani
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Lishui University, Lishui 323000, China.
authors.forename: Muhua
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 317000,China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China.
authors.forename: Jiajie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China. Electronic address: zhuyan@zju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D057048:Plant Nectar / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D009005:Monosaccharides; D009844:Oligosaccharides; D057048:Plant Nectar
keywords: Column-switching; Honeydew; Ion chromatography; Nectar; Sugars