title: Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation.
abstract: OBJECTIVE
  To investigate the effect of the Rho-kinase inhibitor, Y27632, on pig corneal endothelial cell (pCEC) culture, and on inflammation and immune regulation of the responses of human cells to pCECs.
  
  
  METHODS
  pCECs were cultured with/without Y27632 to assess cell proliferation and in vitro wound healing assay. The level of MCP-1 and VEGF in pCECs stimulated with human TNF-α were measured. Proliferation of human PBMCs stimulated with pCECs, and cytokine production in human T cells, and monocyte migration after stimulation were investigated.
  
  
  RESULTS
  Y27632 promoted pCEC proliferation, prevented pCEC death, and enhanced in vitro wound healing. After stimulation, there were significantly lower levels of MCP-1 and VEGF measured in pCECs cultured with Y27632, and significantly reduced human PBMC proliferation, cytokine production, and monocyte migration.
  
  
  CONCLUSIONS
  The application of the Rho-kinase inhibitor will be beneficial when culturing pCECs, and may provide a novel therapy to reduce inflammation after corneal xenotransplantation.
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: Whayoung
authors.initials: W
authors.lastname: Lee
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Miyagawa
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: Cassandra
authors.initials: C
authors.lastname: Long
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: David K C
authors.initials: DK
authors.lastname: Cooper
authors.affiliation: a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hara
mesh_terms: D000577:Amides / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004728:Endothelium, Corneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation / Q000276:immunology*; D007634:Keratitis / Q000276:immunology; D009000:Monocytes / Q000502:physiology; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D051996:Transcription Factor RelA / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D016207:Cytokines; D004791:Enzyme Inhibitors; D011725:Pyridines; D051996:Transcription Factor RelA; D042461:Vascular Endothelial Growth Factor A; C108830:Y 27632; D054460:rho-Associated Kinases
keywords: Corneal endothelial cell; Rho-kinase; immune response; inflammation; pig; xenotransplantation