title: A highly sensitive fluorescence turn-on platform with silver nanoparticles aptasening for human platelet-derived growth factor-BB.
abstract: In this paper, we demonstrated a simple and highly sensitive fluorescence platform for protein detection. Silver nanoparticles (AgNPs) worked as carriers and quenchers for FAM labeled aptamers (FAM-apt). Biotin labeled aptamers (Bio-apt), FAM-apt functionalized AgNPs (Ag-FAM-apt), and a target protein, human platelet-derived growth factor-BB (PDGF-BB) could form a sandwich-type complex. Once the etching solvents were added, AgNPs were dissolved and the fluorescence resonance energy transfer (FRET) between AgNPs and FAM was broken. FAM-apt were no longer quenched and released into the solution in the 96-well microplates, so the fluorescence signal would turn from "off" state to "on" state. This method had possessed several advantages: Firstly, increased specificity which was contributed by the sandwich binding of aptamers; Secondly, quenching ability of AgNPs which was utilized to make signal turn-on; Thirdly, high throughout in which 96 samples could be detected simultaneously. The results showed a linear relationship between fluorescence intensity and PDGF-BB concentration (10 ng mL(-1)-100 ng mL(-1)), and the detection limit was 7 ng mL(-1). This simple and sensitive method would have a promising future for development and application.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, China. Electronic address: lihui@nju.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing 210093, China. Electronic address: xudanke@nju.edu.cn.
authors.forename: Danke
authors.initials: D
authors.lastname: Xu
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry*; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis* / Q000378:metabolism; D012834:Silver / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D012997:Solvents; D000077214:Becaplermin; D012834:Silver
keywords: Aptamer; Etching solvents; Fluorescence turn-on; Platelet-derived growth factor-BB; Silver nanoparticles