title: Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray.
abstract: OBJECTIVE
  To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN).
  
  
  METHODS
  MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription-polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs' functions were predicted through coexpressed mRNA annotations.
  
  
  RESULTS
  A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription-polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes.
  
  
  CONCLUSIONS
  This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.
authors.affiliation: The Second Affiliated Hospital of Nanjing Medical University, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Rui
authors.affiliation: The Second Affiliated Hospital of Nanjing Medical University, China.
authors.forename: Zipeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: The Second Affiliated Hospital of Nanjing Medical University, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: The Second Affiliated Hospital of Nanjing Medical University, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: He
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D008198:Lymph Nodes / Q000737:chemistry* / Q000473:pathology*; D008207:Lymphatic Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: Colorectal cancer; Long noncoding RNA; Lymph node metastasis; Microarray