title: Effects of flow on LOX-1 and oxidized low-density lipoprotein interactions in brain endothelial cell cultures.
abstract: Fluid shear stress and uptake of oxidized low-density lipoprotein (ox-LDL) into the vessel wall both contribute to atherosclerosis, but the relationship between shear stress and ox-LDL uptake is unclear. We examined the effects of flow, induced by orbital rotation of bEnd.3 brain endothelial cell cultures for 1 wk, on ox-LDL receptor (LOX-1) protein expression, ox-LDL uptake and ox-LDL toxicity. Orbitally rotated cultures showed no changes in LOX-1 protein expression, ox-LDL uptake or ox-LDL toxicity, compared to stationary cultures. Flow alone does not modify ox-LDL/LOX-1 signaling in bEnd.3 brain endothelial cells in vitro, suggesting that susceptibility of atheroprone vascular sites to lipid accumulation is not due solely to effects of altered flow on endothelium.
authors.affiliation: Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Mao
authors.affiliation: Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Xie
authors.affiliation: Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA. Electronic address: dgreenberg@buckinstitute.org.
authors.forename: David A
authors.initials: DA
authors.lastname: Greenberg
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D002537:Intracranial Arteriosclerosis / Q000378:metabolism / Q000503:physiopathology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D051127:Scavenger Receptors, Class E / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D008077:Lipoproteins, LDL; C495138:Olr1 protein, mouse; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein
keywords: Atherosclerosis; Brain endothelial cell; Fluid shear stress; Ox-LDL receptor (LOX-1); Oxidized low-density lipoprotein (ox-LDL)