title: Protective effect of diet supplemented with rice prolamin extract against DNCB-induced atopic dermatitis in BALB/c mice.
abstract: BACKGROUND
  Rice prolamin has been reported to possess antioxidative, anti-inflammatory and immune-promoting properties. This study is aimed to examine the protective effects of dietary rice prolamin extract (RPE) against dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in mice.
  
  
  METHODS
  BALB/c mice were fed diet supplemented with 0-0.1 % RPE for 6 weeks. For the last 2 weeks, 1 % or 0.2 % DNCB was applied repeatedly to the back skin of mice to induce AD-like lesions. Following AD induction, the severity of skin lesions was examined macroscopically and histologically. In addition, the serum levels of IgE, IgG1 and IgG2a were determined by ELISA, and the mRNA expression of IL-4 and IFN-γ in the skin was determined by real-time PCR.
  
  
  RESULTS
  Dietary RPE suppressed the clinical symptoms of DNCB-induced dermatitis as well as its associated histopathological changes such as epidermal hyperplasia and infiltration of mast cells and eosinophils in the dermis. RPE treatment also suppressed the DNCB-induced increase in transepidermal water loss. Dietary RPE inhibited the DNCB-induced enhancement of serum IgE and IgG1 levels, whereas it increased the serum IgG2a level in DNCB-treated mice. In addition, dietary RPE upregulated the IFN-γ mRNA expression and downregulated the IL-4 mRNA expression in the skin of DNCB-treated mice.
  
  
  CONCLUSIONS
  The above results suggest that dietary RPE exerts a protective effect against DNCB-induced AD in mice via upregulation of Th1 immunity and that RPE may be useful for the treatment of AD.
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Hyun-Joong
authors.initials: HJ
authors.lastname: Yoon
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Jang
authors.affiliation: Research Center for Oral Disease Regulation of the Aged (MRC), College of Dentistry, Chosun University, Gwangju, South Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Dong-Up
authors.initials: DU
authors.lastname: Song
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Kwang-Il
authors.initials: KI
authors.lastname: Nam
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Choon-Sang
authors.initials: CS
authors.lastname: Bae
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Seong-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea.
authors.forename: Seung-Rock
authors.initials: SR
authors.lastname: Lee
authors.affiliation: COTDE Inc. 19-3, Ugakgol-gil, Susin-myeon, Cheonan-si, Chungcheongnam-do, 330-882, South Korea.
authors.forename: Chang-Sub
authors.initials: CS
authors.lastname: Ku
authors.affiliation: COTDE Inc. 19-3, Ugakgol-gil, Susin-myeon, Cheonan-si, Chungcheongnam-do, 330-882, South Korea.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Jang
authors.affiliation: Chonnam University Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea. bwahn@jnu.ac.kr.
authors.forename: Bong-Whan
authors.initials: BW
authors.lastname: Ahn
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D003876:Dermatitis, Atopic / Q000097:blood / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012275:Oryza; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D055315:Prolamins / Q000494:pharmacology / Q000627:therapeutic use*; D012867:Skin / Q000187:drug effects*
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D055315:Prolamins