title: Eri silkworm (Samia ricini), a non-mulberry host system for AcMNPV mediated expression of recombinant proteins.
abstract: The baculovirus expression system (BVES) based on Autographa californica nucleopolyhedrovirus (AcMNPV) is widely used for the expression of eukaryotic proteins. Several insect cells/larvae that are permissive to AcMNPV have been routinely used as hosts to express heterologous proteins. Domesticated Eri silkworm (Samia ricini), reared in many parts of India, Japan and China, is a non-mulberry silkworm. The present study shows that the Eri silkworm larvae are susceptible to intra-haemocoelical inoculation of AcMNPV. The virus replicates in the larva, as indicated by an increased viral loads in the haemolymph upon injection of a recombinant AcMNPV carrying green fluorescent protein gene. The virus showed localized replication in different tissues including the fat body, haemocytes, tracheal matrix and in the Malphigian tubules. The larval system was successfully used to express heterologous protein, by infecting with a recombinant AcMNPV carrying the 3ABC coding sequence of foot-and-mouth disease virus (FMDV). The study shows that the Eri silkworm larva can be a potential alternative bioreactor, for scaling up of the recombinant proteins employing the baculovirus system.
authors.affiliation: ICAR - Indian Veterinary Research Institute, Hebbal, Bengaluru 560024, India. Electronic address: madhu.hosa@gmail.com.
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Hosamani
authors.affiliation: ICAR - Indian Veterinary Research Institute, Hebbal, Bengaluru 560024, India.
authors.forename: Suresh H
authors.initials: SH
authors.lastname: Basagoudanavar
authors.affiliation: ICAR - Indian Veterinary Research Institute, Hebbal, Bengaluru 560024, India.
authors.forename: B P
authors.initials: BP
authors.lastname: Sreenivasa
authors.affiliation: National Institute of Animal Health, 3-1-5 Kan-non-dai, Tsukuba, Ibaraki 305-0856, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Inumaru
authors.affiliation: ICAR-National Bureau of Agricultural Insect Resources, Hebbal, Bengaluru 560024, India.
authors.forename: Chandish R
authors.initials: CR
authors.lastname: Ballal
authors.affiliation: ICAR - Indian Veterinary Research Institute, Hebbal, Bengaluru 560024, India.
authors.forename: Ramamurthy
authors.initials: R
authors.lastname: Venkataramanan
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism; D019149:Bioreactors; D012831:Bombyx / Q000378:metabolism*; D002460:Cell Line; D005216:Fat Body / Q000378:metabolism; D007814:Larva / Q000821:virology; D031625:Morus / Q000378:metabolism*; D017924:Nucleopolyhedroviruses / Q000254:growth & development / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000941:Antigens; D011994:Recombinant Proteins
keywords: AcMNPV; Baculovirus; Bioreactor; Eri silkworm (Samia ricini); FMDV 3ABC; Infection