title: Evaluation of hydrogel matrices for vessel bioplotting: Vascular cell growth and viability.
abstract: Developing matrices biocompatible with vascular cells is one of the most challenging tasks in tissue engineering. Here, we compared the growth of vascular cells on different hydrogels as potential materials for bioplotting of vascular tissue. Formulations containing alginate solution (Alg, 2%, w/v) blended with protein solutions (silk fibroin, gelatin, keratin, or elastin) at 1% w/v were prepared. Human umbilical vein endothelial cells (ECs), smooth muscle cells (SMCs), and fibroblasts were cultivated on hydrogels for 7 days. Cell number and morphology was visualised using fluorescent staining at day 3 and 7. Cell metabolic activity was analysed using WST assay. Compared to pure Alg, Alg/keratin, Alg/gelatin and Alg/silk fibroin provided superb surfaces for ECs, supporting their attachment, growth, spreading and metabolic activity. SMCs showed best colonization and growth on Alg/silk fibroin and Alg/keratin hydrogels, whereas on elastin-containing hydrogels, cell clustering was observed. Fibroblasts growth was enhanced on Alg/elastin, and strongly improved on silk fibroin- and keratin-containing hydrogels. In contrast to the previous studies with alginate dialdehyde-gelatin crosslinked gels, Alg/gelatin blend hydrogels provided a less favourable scaffold for fibroblasts. Taken together, the most promising results were obtained with silk fibroin- and keratin-containing hydrogels, which supported the growth of all types of vascular cells. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 577-585, 2016.
authors.affiliation: Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Foundation-endowed Professorship for Nanomedicine, ENT Department, University Hospital Erlangen, Germany.|Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
authors.forename: Raminder
authors.initials: R
authors.lastname: Singh
authors.affiliation: Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Bapi
authors.initials: B
authors.lastname: Sarker
authors.affiliation: Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Raquel
authors.initials: R
authors.lastname: Silva
authors.affiliation: Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Detsch
authors.affiliation: Department of Cardiology and Angiology, University Hospital Erlangen, Erlangen, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dietel
authors.affiliation: Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Foundation-endowed Professorship for Nanomedicine, ENT Department, University Hospital Erlangen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
authors.affiliation: Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.affiliation: Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Foundation-endowed Professorship for Nanomedicine, ENT Department, University Hospital Erlangen, Germany.
authors.forename: Iwona
authors.initials: I
authors.lastname: Cicha
mesh_terms: D000464:Alginates / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001808:Blood Vessels / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid
keywords: alginate-protein hydrogels; biocompatibility; fibroblasts; scaffold endothelialisation; smooth muscle cells