title: A disposable electrochemical sensor based on protein G for High-Density Lipoprotein (HDL) detection.
abstract: In this work, two biosensors were developed for the detection of High-Density Lipoproteins (HDL) particles, which are biomarkers inversely correlated with cardiovascular risk and which represent therapeutic targets for atherosclerosis. The electrochemical properties of the grafted antibody on interdigitated gold electrode were achieved by Impedance Spectroscopy (IS). The used deposition method was based on oriented antibody Anti-ApoA1 with an intermediate thin layer of protein G. The developed biosensor was able to detect both native plasma HDL and reconstituted HDL (rHDL) particles respectively with the detection limit of 50n g/mL and 1 ng/mL, respectively. Dynamic contact angle and atomic force microscopy were used. The developed biosensors are able to differentiate the HDL particles according to their differences in size and interactions with the immobilized antibody.
authors.affiliation: Carthage University, National Institute of Applied Science and Technology, Physics Department and Instrumentation, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia.
authors.forename: H
authors.initials: H
authors.lastname: Chammem
authors.affiliation: Carthage University, National Institute of Applied Science and Technology, Physics Department and Instrumentation, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Hafaid
authors.affiliation: Carthage University, National Institute of Applied Science and Technology, Physics Department and Instrumentation, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Bohli
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, Laboratoire des Sciences des Procédés et des Matériaux, CNRS (UPR 3407), F-93430 Villetaneuse, France.
authors.forename: A
authors.initials: A
authors.lastname: Garcia
authors.affiliation: Inserm U1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, CHU -CIC de La Réunion, Saint-Denis, France.
authors.forename: O
authors.initials: O
authors.lastname: Meilhac
authors.affiliation: Carthage University, National Institute of Applied Science and Technology, Physics Department and Instrumentation, Centre Urbain Nord, Bp676, 1080 Charguia Cedex, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Abdelghani
authors.affiliation: INSERM U1148, LVTS, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, Villetaneuse, France. Electronic address: laurence.mora@univ-paris13.fr.
authors.forename: L
authors.initials: L
authors.lastname: Mora
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000941:Antigens / Q000276:immunology; D016632:Apolipoprotein A-I / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000097:blood / Q000276:immunology; D018625:Microscopy, Atomic Force; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: C505693:APOA1 protein, human; D055910:Antibodies, Immobilized; D000941:Antigens; D016632:Apolipoprotein A-I; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D008075:Lipoproteins, HDL; D012710:Serum Albumin, Bovine; D006046:Gold
keywords: AFM; Contact angle; HDL; Impedance spectroscopy.; Protein G