title: FTO polymorphism, cardiorespiratory fitness, and obesity in Brazilian youth.
abstract: The aim of the present study was to evaluate the relationship between the rs9939609 fat mass and obesity-associated (FTO) polymorphism and cardiorespiratory fitness (CRF) with overweight/obesity outcomes in youth.
  
  
  
  This study included 420 youths, comprising 211 boys and 209 girls aged 7-17. Overweight/obesity were evaluated by body mass index (BMI), waist circumference (WC), and the percentage of fat (PF) according to two skinfold thickness measurements. Genotyping of the rs9939609 polymorphism was conducted using real-time Polymerase Chain Reaction (PCR) utilizing TaqMan(®) probes, and CRF was evaluated through a 9-minute run/walk test, categorized as fit or unfit. Logistic regression was utilized to evaluate a possible association between the polymorphism and CRF, with three obesity indicators evaluated.
  
  
  
  Individuals with the genotype risk (AA) of FTO polymorphism rs9939609 showed higher prevalence of overweight/obesity, as evaluated by BMI (OR: 3.21; CI: 1.71-6.05), WC (OR: 2.59; CI: 1.35-4.97), and PF (OR: 2.59; CI: 1.36-4.92). Additionally, students with the AA genotype in the unfit model had a significant odds ratio for obesity (OR: 4.40; CI: 1.83-10.61 for BMI; OR: 3.54; CI: 1.58-7.96 for WC), whereas we did not observe associations between the AA genotype with BMI and WC using the fit model. Conversely, PF was associated with the AA genotype only in the fit model (OR: 3.24; CI: 1.26-8.34).
  
  
  
  This study demonstrated that the rs9939609 (FTO) polymorphism showed a relationship with obesity in the population studied and an interaction with CRF. Students with low levels of CRF and the AA genotype have a higher risk of being overweight/obese. This association was not found in students with higher levels of CRF. Am. J. Hum. Biol. 28:381-386, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Physical Education and Health, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Cézane Priscila
authors.initials: CP
authors.lastname: Reuter
authors.affiliation: Department of Biology and Pharmacy, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Andréia
authors.initials: A
authors.lastname: Rosane De Moura Valim
authors.affiliation: Department of Physical Education, Physical Education School, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, 90690-200, Brazil.
authors.forename: Anelise Reis
authors.initials: AR
authors.lastname: Gaya
authors.affiliation: Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Tássia Silvana
authors.initials: TS
authors.lastname: Borges
authors.affiliation: Academic of Pharmacy, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Elisa Inês
authors.initials: EI
authors.lastname: Klinger
authors.affiliation: Department of Biology and Pharmacy, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Lia Gonçalves
authors.initials: LG
authors.lastname: Possuelo
authors.affiliation: Department of Physical Education and Health, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Silvia Isabel Rech
authors.initials: SI
authors.lastname: Franke
authors.affiliation: Department of Molecular Biology and Biotechnology, Center for Biotechnology, Post-Graduation Program in Cellular and Molecular Biology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Lívia
authors.initials: L
authors.lastname: Kmetzsch
authors.affiliation: Department of Molecular Biology and Biotechnology, Center for Biotechnology, Post-Graduation Program in Cellular and Molecular Biology, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Marilene Henning
authors.initials: MH
authors.lastname: Vainstein
authors.affiliation: Department of Biology and Pharmacy, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Daniel
authors.initials: D
authors.lastname: Prá
authors.affiliation: Department of Physical Education and Health, Postgraduate Program in Health Promotion, University of Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, 96815-900, Brazil.
authors.forename: Miria Suzana
authors.initials: MS
authors.lastname: Burgos
mesh_terms: D000293:Adolescent; D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO / Q000235:genetics* / Q000378:metabolism; D001938:Brazil; D000072599:Cardiorespiratory Fitness / Q000502:physiology*; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D063766:Pediatric Obesity / Q000453:epidemiology* / Q000235:genetics / Q000503:physiopathology; D011110:Polymorphism, Genetic; D015995:Prevalence
chemical_list: D000071516:Alpha-Ketoglutarate-Dependent Dioxygenase FTO; C526492:FTO protein, human