title: Isolation and identification of bat viruses closely  related to human, porcine and mink  orthoreoviruses.
abstract: Bats have been identified as natural reservoirs of many viruses, including reoviruses. Recent studies have demonstrated the interspecies transmission of bat reoviruses to humans. In this study, we report the isolation and molecular characterization of six strains of mammalian orthoreovirus (MRV) from Hipposideros and Myotis spp. These isolates were grouped into MRV serotype 1, 2 or 3 based on the sequences of the S1 gene, which encodes the outer coat protein s1. Importantly, we found that three of six bat MRV strains shared high similarity with MRVs isolated from diseased minks, piglets or humans based on the S1 segment, suggesting that interspecies transmission has occurred between bats and humans or animals. Phylogenetic analyses based on the 10 segments showed that the genomic segments of these bat MRVs had different evolution lineages, suggesting that these bat MRVs may have arisen through reassortment of MRVs of different origins.
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Xing-Lou
authors.initials: XL
authors.lastname: Yang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tan
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Chu-Ming
authors.initials: CM
authors.lastname: Luo
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Mei-Niang
authors.initials: MN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Xing-Yi
authors.initials: XY
authors.lastname: Ge
authors.affiliation: Guangdong Entomological Institute, Guangzhou, PR China.
authors.forename: Li-Biao
authors.initials: LB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, PR China.
authors.forename: Zheng-Li
authors.initials: ZL
authors.lastname: Shi
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D002685:Chiroptera / Q000821:virology*; D004197:Disease Reservoirs / Q000821:virology; D006801:Humans; D008907:Mink / Q000821:virology*; D030102:Orthoreovirus, Mammalian / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012088:Reoviridae Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D065288:Serogroup; D013552:Swine / Q000821:virology*