title: Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin.
abstract: The skin is a rich source of readily accessible stem cells. The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolation from skin; however, none of them afforded simultaneous isolation of more than one population. Herein, we describe the simultaneous isolation and characterization of three stem cell populations from the dermis and epidermis of murine skin, namely Epidermal Stem Cells (EpiSCs), Skin-derived Precursors (SKPs) and Mesenchymal Stem Cells (MSCs). The simultaneous isolation was possible through a simple protocol based on culture selection techniques. These cell populations are shown to be capable of generating chondrocytes, adipocytes, osteocytes, terminally differentiated keratinocytes, neurons and glia, rendering this protocol suitable for the isolation of cells for tissue replenishment and cell based therapies. The advantages of this procedure are far-reaching since the skin is not only the largest organ in the body, but also provides an easily accessible source of stem cells for autologous graft.
authors.affiliation: Núcleo de Terapia Celular e Molecular (NUCEL/NETCEM), Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, 05360-130 SP, Brasil; Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, São Paulo, 05508-000 SP, Brasil.
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Forni
authors.affiliation: Núcleo de Terapia Celular e Molecular (NUCEL/NETCEM), Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, 05360-130 SP, Brasil.
authors.forename: Aline
authors.initials: A
authors.lastname: Ramos Maia Lobba
authors.affiliation: Núcleo de Terapia Celular e Molecular (NUCEL/NETCEM), Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, 05360-130 SP, Brasil.
authors.forename: Alexandre Hamilton
authors.initials: AH
authors.lastname: Pereira Ferreira
authors.affiliation: Núcleo de Terapia Celular e Molecular (NUCEL/NETCEM), Departamento de Clínica Médica, Faculdade de Medicina, Universidade de São Paulo, São Paulo, 05360-130 SP, Brasil; Instituto de Química, Departamento de Bioquímica, Universidade de São Paulo, São Paulo, 05508-000 SP, Brasil.
authors.forename: Mari Cleide
authors.initials: MC
authors.lastname: Sogayar
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D024141:Collagen Type IV / Q000494:pharmacology; D020405:Dermis / Q000166:cytology; D000078404:Epidermal Cells; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008807:Mice, Inbred BALB C; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014404:Tubulin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D024141:Collagen Type IV; D005904:Glial Fibrillary Acidic Protein; D014404:Tubulin; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases