title: Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis.
abstract: This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.
authors.forename: P K
authors.initials: PK
authors.lastname: Balne
authors.forename: S
authors.initials: S
authors.lastname: Basu
authors.forename: S
authors.initials: S
authors.lastname: Rath
authors.forename: M R
authors.initials: MR
authors.lastname: Barik
authors.affiliation: Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India.
authors.forename: S
authors.initials: S
authors.lastname: Sharma
mesh_terms: D003365:Costs and Cost Analysis; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014392:Tuberculosis, Ocular / Q000175:diagnosis*