title: A small-scale experiment of using phage-based probiotic dietary supplement for prevention of E. coli traveler's diarrhea.
abstract: Traveler's diarrhea (TD) is caused by Escherichia coli in 30% of cases. We have developed a phage cocktail for prophylaxis of TD caused by E.coli, Shigella flexneri, Shigella sonnei, Salmonella enterica, Listeria monocytogenes or Staphylococcus aureus, and investigated its effectiveness against infection caused by the non-pathogenic Lac (-) strain of E.coli K12 C600 in animal and human trials. On the 6th day of both animal and human trials E. coli K12 C600 strain was detected in titer of 104CFU/g of mice feces and 106CFU/g of human feces in the control (untreated) groups, while it was not detected in the samples of either of the study (phage-treated) groups. These results have great significance because the original coliphages included in the cocktail have a broad host-range including ETEC, EAEC and EHEC strains which cause severe cases of TD.
authors.affiliation: Bphage LLC ; Moscow, Russia ; Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Aleshkin
authors.affiliation: Bphage LLC ; Moscow, Russia ; Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: E O
authors.initials: EO
authors.lastname: Rubalskii
authors.affiliation: State Research Center for Applied Microbiology & Biotechnology ; Obolensk , Moscow region, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Volozhantsev
authors.affiliation: State Research Center for Applied Microbiology & Biotechnology ; Obolensk , Moscow region, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Verevkin
authors.affiliation: State Research Center for Applied Microbiology & Biotechnology ; Obolensk , Moscow region, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Svetoch
authors.affiliation: Bphage LLC ; Moscow, Russia ; Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Kiseleva
authors.affiliation: Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Bochkareva
authors.affiliation: Bphage LLC ; Moscow, Russia ; Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: O Yu
authors.initials: OY
authors.lastname: Borisova
authors.affiliation: Bphage LLC ; Moscow, Russia ; State Research Center for Applied Microbiology & Biotechnology ; Obolensk , Moscow region, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Popova
authors.affiliation: State Research Center for Applied Microbiology & Biotechnology ; Obolensk , Moscow region, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Bogun
authors.affiliation: Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.
authors.forename: S S
authors.initials: SS
authors.lastname: Afanas'ev
keywords: EAEC; EHEC; ETEC; phage prophylaxis; phage-based probiotic dietary supplement; traveler's diarrhea