title: [Effect of HIF-1α Gene Silence on Biological Characteristics of Human Colon Cancer Cells SW480].
abstract: OBJECTIVE
  To investigate changes in proliferation and apoptosis of human colon cancer SW480 cells after silencing hypoxia inducible factor-lα (HIF-1α) expression.
  
  
  METHODS
  siRNA interference technology was performed to silence the expression of HIF-1α using lipofectamine mediation to transfect siRNA into human colon cancer SW480 cells. The siRNA interfered SW480 cells were compared with a negative control group, an empty vector group, and a blank control group. Real-time PCR and Western blot were used to measure the expressions of HIF-lα protein and mRNA. MTT and flow cytometry (FCM) were used to evaluate the apoptosis and proliferation of SW480 cells.
  
  
  RESULTS
  The interfered SW480 cells had a higher level of silence of HIF-lα mRNA (> 80%) compared with those of in the three control groups (P < 0.05). Higher levels of apoptosis and lower levels of proliferation were detected in the interfered cells compared with those in the control groups (P < 0.01).
  
  
  CONCLUSIONS
  HIF-lα silencing promotes apoptosis and inhibits the proliferation of SW480 cells in vitro.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Lang-song
authors.initials: LS
authors.lastname: Hao
authors.forename: Jia
authors.initials: J
authors.lastname: Shi
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D034741:RNA, Small Interfering