title: The fluorescence detection of glutathione by ∙OH radicals' elimination with catalyst of MoS2/rGO under full spectrum visible light irradiation.
abstract: In this study, a new method for the detection of glutathione (GSH) was designed based on the ∙OH radicals' elimination system due to the reducing ability of GSH for the first time. Fluorescence method with terephthalic acid (TA) as the probe was employed for the quantification of ∙OH radicals' production and elimination. Experimental conditions of ∙OH radicals' production were optimized in detail, and ∙OH radicals were found to be efficiently produced by the excellent catalysis performance of MoS2/rGO under full spectrum visible light irradiation. The introduction of GSH make fluorescent intensity decrease due to the elimination of ∙OH radicals. For the present fluorescence based GSH sensor, a wide detection range of 60.0-700.0 µM and excellent selectivity have been achieved. Furthermore, it has been successfully employed for the determination of GSH in commercial drug tablets and human serum.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Weiguang
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
authors.forename: Dongxue
authors.initials: D
authors.lastname: Han
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Lingnan
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China. Electronic address: tswu@ciac.ac.cn.
authors.forename: Tongshun
authors.initials: T
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
authors.forename: Li
authors.initials: L
authors.lastname: Niu
mesh_terms: D002384:Catalysis; D004220:Disulfides / Q000737:chemistry* / Q000528:radiation effects; D005453:Fluorescence; D005978:Glutathione / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D006108:Graphite / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry*; D008027:Light; D008982:Molybdenum / Q000737:chemistry* / Q000528:radiation effects; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry* / Q000528:radiation effects; D010795:Phthalic Acids / Q000737:chemistry; D013607:Tablets / Q000737:chemistry
chemical_list: D004220:Disulfides; D010087:Oxides; D010795:Phthalic Acids; D013607:Tablets; D017665:Hydroxyl Radical; C011363:terephthalic acid; D006108:Graphite; D008982:Molybdenum; D005978:Glutathione; C082964:molybdenum disulfide
keywords: Fluorescent assay; Glutathione; MoS(2)/rGO; ∙OH radical