title: Dengue-3 Virus Entry into Vero Cells: Role of Clathrin-Mediated Endocytosis in the Outcome of Infection.
abstract: The endocytic uptake and intracellular trafficking for penetration of DENV-3 strain H-87 into Vero cells was analyzed by using several biochemical inhibitors and dominant negative mutants of cellular proteins. The results presented show that the infective entry of DENV-3 into Vero cells occurs through a non-classical endocytosis pathway dependent on low pH and dynamin, but non-mediated by clathrin. After uptake, DENV-3 transits through early endosomes to reach Rab 7-regulated late endosomes, and according with the half-time for ammonium chloride resistance viral nucleocapsid is released into the cytosol approximately at 12 min post-infection. Furthermore, the influence of the clathrin pathway in DENV-3 infective entry in other mammalian cell lines of human origin, such as A549, HepG2 and U937 cells, was evaluated demonstrating that variable entry pathways are employed depending on the host cell. Results show for the first time the simultaneous coexistence of infective and non -infective routes for DENV entry into the host cell, depending on the usage of clathrin-mediated endocytosis.
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; IQUIBICEN, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
authors.forename: Luana E
authors.initials: LE
authors.lastname: Piccini
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Viviana
authors.initials: V
authors.lastname: Castilla
authors.affiliation: Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; IQUIBICEN, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
authors.forename: Elsa B
authors.initials: EB
authors.lastname: Damonte
mesh_terms: D000330:Aedes; D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D002966:Clathrin / Q000378:metabolism*; D003715:Dengue / Q000473:pathology / Q000821:virology*; D003716:Dengue Virus / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D056945:Hep G2 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D011379:Prognosis; D020298:U937 Cells; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D002966:Clathrin