title: A new kaurane diterpenoid from Isodon inflexus.
abstract: A new 7,20-epoxy kaurane diterpenoid, 15-acetyldemethylkamebacetal A (1) and six known kaurane diterpenoids (2-7) were isolated from the aerial parts of Isodon inflexus in nuclear transcription factor-κB (NF-κB)-dependent reporter gene assay-guided fractionation. Their chemical structures were determined on the basis of extensive spectroscopic analysis (UV, IR, MS, 1D- and 2D-NMR) and comparison with literature data. The isolated compounds were evaluated for their inhibitory effects on TNF-α-induced NF-κB activation, and all compounds exhibited NF-κB inhibitory activities with IC50 values ranging from 1.91 to 20.15 μM.
authors.affiliation: a Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy , Yanbian University , Yanji , P.R. China.|b Molecular Medicine Research Center , Yanbian University , Yanji , P.R. China.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Xu
authors.affiliation: a Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy , Yanbian University , Yanji , P.R. China.
authors.forename: Xing-Fu
authors.initials: XF
authors.lastname: Cai
authors.affiliation: a Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy , Yanbian University , Yanji , P.R. China.|b Molecular Medicine Research Center , Yanbian University , Yanji , P.R. China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Jin
authors.affiliation: b Molecular Medicine Research Center , Yanbian University , Yanji , P.R. China.
authors.forename: Jung Joon
authors.initials: JJ
authors.lastname: Lee
mesh_terms: D045786:Diterpenes, Kaurane / Q000032:analysis*; D017930:Genes, Reporter / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D037602:Isodon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D016328:NF-kappa B / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
chemical_list: C000611780:15-acetyldemethylkamebacetal A; D045786:Diterpenes, Kaurane; D016328:NF-kappa B; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: Isodon inflexus; NF-κB; kaurane diterpenoid; structure elucidation