title: Hapten-Binding Bispecific Antibodies for the Targeted Delivery of SiRNA and SiRNA-Containing Nanoparticles.
abstract: Hapten-binding bispecific antibodies (bsAbs) are effective and versatile tools for targeting diverse payloads, including siRNAs, to specific cells and tissues. In this chapter, we provide examples for successful SiRNA delivery using this powerful targeting platform. We further provide protocols for designing and producing bsAbs, for combining bsAbs with SiRNA into functional complexes, and achieving specific mRNA knockdown in cells by using these functional complexes.
authors.affiliation: Roche Pharma Research & Early Development, Large Molecule Research, Roche Innovation Center Penzberg, Nonnenwald 2, 82377, Penzberg, Germany.
authors.forename: Irmgard S
authors.initials: IS
authors.lastname: Thorey
authors.affiliation: Roche Pharma Research & Early Development, Large Molecule Research, Roche Innovation Center Penzberg, Nonnenwald 2, 82377, Penzberg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Grote
authors.affiliation: Roche Pharma Research & Early Development, Large Molecule Research, Roche Innovation Center Penzberg, Nonnenwald 2, 82377, Penzberg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Mayer
authors.affiliation: Roche Pharma Research & Early Development, Large Molecule Research, Roche Innovation Center Penzberg, Nonnenwald 2, 82377, Penzberg, Germany. ulrich.brinkmann@roche.com.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Brinkmann
mesh_terms: D018033:Antibodies, Bispecific / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004337:Drug Carriers / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors / Q000235:genetics; D006241:Haptens / Q000276:immunology*; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D018033:Antibodies, Bispecific; D004337:Drug Carriers; D006241:Haptens; D034741:RNA, Small Interfering
keywords: Bispecific antibodies; Dynamic polyconjugates; Fab; Hapten; IgG; Lipid nanoparticles; Recombinant antibody expression; SiRNA delivery; Targeted delivery; scFv