title: S100β Levels in CSF of Nonambulatory Dogs with Intervertebral Disk Disease Treated with Electroacupuncture.
abstract: The aim of the study was to investigate S100β levels in the cerebrospinal fluid of nonambulatory dogs with intervertebral disk disease treated with electroacupuncture: 10 dogs with thoracolumbar disk extrusion graded 3 to 5 (EA group) and 7 dogs without neurologic dysfunction (control group). All dogs regained ambulation. S100β was detected by Western blot analysis where EA group dogs were evaluated at two time points (M1 = before EA and M2 = when the dogs return ambulation) and at one time point from control group. In EA group dogs M1-S100β levels were significantly higher than in control group. EA group dogs were divided into subgroups A (n = 7-early motor recovery; 6.7 ± 7.8 days) and B (n = 3-late motor recovery; 76 ± 17.0 days). M1-S100β levels were similar between subgroups A and B. However, M2-S100β levels were significantly higher in subgroup B than in subgroup A. An elevated S100β levels were observed in dogs with late motor recovery. S100β may be associated with neuroplasticity following spinal cord injuries with intervertebral disk extrusion. Further studies with larger numbers of subjects and control group with affected dogs are necessary to investigate the relationship between neurotrophic factors and electroacupuncture stimulation.
authors.affiliation: Department of Surgery, School of Veterinary Medicine and Animal Science, The University of São Paulo, 05508-270 São Paulo, SP, Brazil.
authors.forename: Ayne
authors.initials: A
authors.lastname: Murata Hayashi
authors.affiliation: Department of Surgery, School of Veterinary Medicine and Animal Science, The University of São Paulo, 05508-270 São Paulo, SP, Brazil.
authors.forename: Ana Carolina Brandão Campos
authors.initials: AC
authors.lastname: Fonseca Pinto
authors.affiliation: Department of Surgery, School of Veterinary Medicine and Animal Science, The University of São Paulo, 05508-270 São Paulo, SP, Brazil.
authors.forename: Silvia Renata Gaido
authors.initials: SR
authors.lastname: Cortopassi
authors.affiliation: Center of Mathematics, Computation and Cognition, The Federal University of ABC, 09210-170 Santo André, SA, Brazil.
authors.forename: Valdecir
authors.initials: V
authors.lastname: Marvulle
authors.affiliation: Department of Neurology, School of Medicine, The University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Jessica
authors.initials: J
authors.lastname: Ruivo Maximino
authors.affiliation: Department of Neurology, School of Medicine, The University of São Paulo, 01246-903 São Paulo, SP, Brazil.
authors.forename: Gerson
authors.initials: G
authors.lastname: Chadi
authors.affiliation: Department of Surgery, School of Veterinary Medicine and Animal Science, The University of São Paulo, 05508-270 São Paulo, SP, Brazil.
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Matera