title: Effect of β-cyclodextrin on the internalization of nanoparticles into intestine epithelial cells.
abstract: The influence of β-cyclodextrin on the interaction and internalization of PLGA nanoparticles into intestine epithelial cells was assessed. For this purpose β-cyclodextrin was adsorbed on PLGA nanoparticles. Interaction of nanoparticles with Caco-2 cells, determined by fluorescence, was expressed as the number of particles per cell. Confocal microscopy confirmed the localization of the particles in the cell monolayer. The results showed that adsorption of β-cyclodextrin on the surface of PLGA nanoparticles reduces interaction with mucin, enhancing in this way the internalization into the Caco-2 cells.
authors.affiliation: División de Estudios de Posgrado (Tecnología Farmacéutica), Universidad Nacional Autónoma de México, Mexico.
authors.forename: Lorena
authors.initials: L
authors.lastname: García-González
authors.affiliation: UIMEIP-Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Mexico.
authors.forename: Lilián
authors.initials: L
authors.lastname: Yépez-Mulía
authors.affiliation: División de Estudios de Posgrado (Tecnología Farmacéutica), Universidad Nacional Autónoma de México, Mexico. Electronic address: ganemq@hotmail.com.
authors.forename: Adriana
authors.initials: A
authors.lastname: Ganem
mesh_terms: D001692:Biological Transport; D018938:Caco-2 Cells; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000166:cytology; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D009077:Mucins / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D047392:beta-Cyclodextrins / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D009077:Mucins; D047392:beta-Cyclodextrins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C031215:betadex
keywords: Caco-2 cells; Nanoparticles; PLGA; β-Cyclodextrin