title: Templated polymers enable selective capture and release of lysophosphatidic acid in human plasma via optimization of non-covalent binding to functional monomers.
abstract: The first solid phase extraction materials for selective lysophosphatidic acid (LPA) enrichment from human plasma are described. Molecularly imprinted polymers were designed, synthesized and evaluated as cartridge fillings. They enabled a relatively rapid and simple extraction protocol for LPA without any need for multiple liquid-liquid extraction steps. The five major subspecies of lysophosphatidic acid are readily separated from all other native plasma phospholipids, including those well-known to interfere with LPA quantitation, such as phosphatidylcholine and lysophosphatidylcholine. Outstanding LPA purity is obtained via these solid phase materials in a tandem extraction setup.
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Jialu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Martha
authors.initials: M
authors.lastname: Sibrian-Vazquez
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Escobedo
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Chu
authors.affiliation: Women and Infants Hospital, Brown University, Providence, RI 02905, USA.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Moore
authors.affiliation: Department of Chemistry, Portland State University, Portland, OR 97201, USA. strongin@pdx.edu.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Strongin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D059625:Liquid-Liquid Extraction; D008246:Lysophospholipids / Q000097:blood* / Q000302:isolation & purification*; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D008246:Lysophospholipids; D011108:Polymers; C032881:lysophosphatidic acid