title: The autophagy GABARAPL1 gene is epigenetically regulated in breast cancer models.
abstract: BACKGROUND
  The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer.
  
  
  METHODS
  In this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications.
  
  
  RESULTS
  Our data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression.
  
  
  CONCLUSIONS
  Our work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells.
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. eric.hervouet@univ-fcomte.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Hervouet
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. taupin.aurore@gmail.com.
authors.forename: Aurore
authors.initials: A
authors.lastname: Claude-Taupin
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. thierry.gauthier59@hotmail.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Gauthier
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. valerie.perez@univ-fcomte.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Perez
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. annick.fraichard@univ-fcomte.fr.
authors.forename: Annick
authors.initials: A
authors.lastname: Fraichard
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. padami@univ-fcomte.fr.
authors.forename: Pascale
authors.initials: P
authors.lastname: Adami
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. gilles.despouy@univ-fcomte.fr.
authors.forename: Gilles
authors.initials: G
authors.lastname: Despouy
authors.affiliation: Department of Pathology, University Hospital Jean-Minjoz, 25030, Besançon, France. fmonnien@chu-besancon.fr.
authors.forename: Franck
authors.initials: F
authors.lastname: Monnien
authors.affiliation: Department of Pathology, University Hospital Jean-Minjoz, 25030, Besançon, France. mp1algros@chu-besancon.fr.
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Algros
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. michele.jouvenot@univ-fcomte.fr.
authors.forename: Michèle
authors.initials: M
authors.lastname: Jouvenot
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. regis.delage-mourroux@univ-fcomte.fr.
authors.forename: Régis
authors.initials: R
authors.lastname: Delage-Mourroux
authors.affiliation: Université de Franche-Comté, Laboratoire de Biochimie, EA3922 « Estrogènes, Expression Génique et Pathologies du Système Nerveux Central », SFR IBCT FED4234, UFR Sciences et Techniques, 16 route de Gray, 25030, Besançon Cedex, France. michael.boyer-guittaut@univ-fcomte.fr.
authors.forename: Michaël
authors.initials: M
authors.lastname: Boyer-Guittaut
mesh_terms: D000107:Acetylation; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D001343:Autophagy / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D057890:Epigenomics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; C430503:GABARAPL1 protein, human; D006657:Histones; D008869:Microtubule-Associated Proteins