title: Recognition of Streptococcus pseudoporcinus Colonization in Women as a Consequence of Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Group B Streptococcus Identification.
abstract: During a 14-month period of using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for group B streptococcus (GBS) identification, we recovered 32 (1%) Streptococcus pseudoporcinus isolates from 3,276 GBS screening cultures from female genital sources (25 isolates from pregnant women and 7 from nonpregnant women). An additional two S. pseudoporcinus isolates were identified from a urine culture and a posthysterectomy wound culture. These isolates were found to cross-react with three different GBS antigen agglutination kits, PathoDx (Remel) (93%), Prolex (Pro-Lab Diagnostics) (38%), and Streptex (Remel) (53%). New approaches to bacterial identification in routine clinical microbiology laboratories may affect the prevalence of S. pseudoporcinus.
authors.affiliation: Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand nsuwantarat@gmail.com.
authors.forename: Nuntra
authors.identifier: http://orcid.org/0000-0002-9356-2592
authors.initials: N
authors.lastname: Suwantarat
authors.affiliation: Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Maureen
authors.initials: M
authors.lastname: Grundy
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Mayer
authors.initials: M
authors.lastname: Rubin
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Renee
authors.initials: R
authors.lastname: Harris
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Jo-Anne
authors.initials: JA
authors.lastname: Miller
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Romagnoli
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Hanlon
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Tsigereda
authors.initials: T
authors.lastname: Tekle
authors.affiliation: Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Ellis
authors.affiliation: Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Frank R
authors.initials: FR
authors.lastname: Witter
authors.affiliation: Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland, USA.
authors.forename: Karen C
authors.initials: KC
authors.lastname: Carroll
mesh_terms: D000293:Adolescent; D000328:Adult; D000372:Agglutination Tests; D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis*; D013291:Streptococcus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification*; D055815:Young Adult