title: Modification of Bacterial Cellulose with Organosilanes to Improve Attachment and Spreading of Human Fibroblasts.
abstract: Bacterial Cellulose (BC) synthesized byAcetobacter xylinumhas been a promising candidate for medical applications. Modifying BC to possess the properties needed for specific applications has been reported. In this study, BCs functionalized by organosilanes were hypothesized to improve the attachment and spreading of Normal Human Dermal Fibroblast (NHDF). The BC gels obtained from biosynthesis were dried by either ambient-air drying or freeze drying. The surfaces of those dried BCs were chemically modified by grafting methyl terminated octadecyltrichlorosilane (OTS) or amine terminated 3-aminopropyltriethoxysilane (APTES) to expectedly increase hydrophobic or electrostatic interactions with NHDF cells, respectively. NHDF cells improved their attachment and spreading on the majority of APTES-modified BCs (∼70-80% of area coverage by cells) with more rapid growth (∼2.6-2.8× after incubations from 24 to 48h) than on tissue culture polystyrene (∼2×); while the inverse results (< 5% of area coverage and stationary growth) were observed on the OTS-modified BCs. For organosilane modified BCs, the drying method had no effect onin vitrocell attachment/spreading behaviors.
authors.affiliation: Department of Chemical Engineering, Chulalongkorn University, Bangkok 10330, Thailand ; Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325-3906, United States.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Taokaew
authors.affiliation: Department of Chemical Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Muenduen
authors.initials: M
authors.lastname: Phisalaphong
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325-3906, United States.
authors.forename: Bi-Min Zhang
authors.initials: BZ
authors.lastname: Newby
keywords: Bacterial cellulose; Fibroblasts; Modification; Organosilanes