title: MiR-451, a potential prognostic biomarker and tumor suppressor for gastric cancer.
abstract: BACKGROUND
  The expression of microRNA-451 (miR-451) and its association with clinical pathological factors in GC remain still unclear. The purpose of this study was to investigate if miR-451 is a potential prognostic biomarker and tumor suppressor for gastric cancer.
  
  
  METHODS
  Real-time quantitative RT-PCR (qRT-PCR) was applied to detect miR-451 expression in GC cell lines and primary tumor and paired non-cancerous tissues. The association of miR-451 expression with clinicopathological factors and prognosis was statistically analyzed.
  
  
  RESULTS
  We found that miR-451 showed decreased expression in GC tissues and cell lines, and its down-regulation tended to be positively correlated with lymphatic metastasis, clinical staging and shorter overall survival of patients. In addition, forced expression of miR-451 in BGC-823 and MKN-45 cells did not impact on cell proliferation, but did reduce migration and invasion rates in BGC-823 cells.
  
  
  CONCLUSIONS
  Our findings indicated that miR-451 may act as a novel prognostic marker and potential therapeutic target in human GC.
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
authors.forename: Zhongxue
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
authors.forename: Zhonghou
authors.initials: Z
authors.lastname: Rong
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
authors.forename: Wenmao
authors.initials: W
authors.lastname: Geng
authors.affiliation: Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Road, Jinan 250021, Shandong Province, China.
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C531201:MIRN451 microRNA, human; D035683:MicroRNAs
keywords: MiR-451; gastric cancer; metastasis; prognosis