title: Structural elucidation of polysaccharide fractions from the brown alga Coccophora langsdorfii and in vitro investigation of their anticancer activity.
abstract: Laminaran, fucoidan, and alginate were isolated from the brown alga Coccophora langsdorfii collected in the Japan Sea. The structural characteristics of polysaccharides were investigated by NMR spectroscopy. The laminaran was determined as β-d-glucan, which consisted of 80% of 1,3- and 20% of 1,6-linked residues and was terminated with mannitol. The alginate was a guluronic acid-rich polysaccharide (M/G=0.85). Fucoidan, sulfated α-l-fucan, contained a linear backbone of alternating (1→3)- and (1→4)- linked α-l-fucopyranose residues with sulfate at C2 and C4 of (1→3)-α-l-fucopyranose residues. Anticancer activity of this fucoidan was investigated in comparison with activity of fucoidan having similar linear backbone from the brown alga Fucus evanescens. The fucoidan from C. langsdorfii significantly inhibited colony formation of SK-MEL-5 and SK-MEL-28 melanoma cells (the percentage of inhibition was 28 and 76, respectively) and weakly inhibited colony formation of breast adenocarcinoma cells MDA-MB-231 (the percentage of inhibition was about 5). Similar results were obtained for fucoidan from F. evanescens; the percentage of inhibition of colony formation of SK-MEL-5 and SK-MEL-28 melanoma cells was 54 and 56, respectively. The inhibition of colony formation of breast adenocarcinoma cells MDA-MB-231 was weak. We suppose that other sulfated and partially acetylated fucoidans consisting of (1→3)- and (1→4)-linked α-l-fucopyranose residues may suppress progression of melanoma cell colony formation similar to fucoidans of C. langsdorfii and F. evanescens.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Laboratory of Enzyme Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 159, Prospect 100-let Vladivostoku 690022, Russia. Electronic address: tatyanaimbs@mail.ru.
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Imbs
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Laboratory of Enzyme Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 159, Prospect 100-let Vladivostoku 690022, Russia.
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Ermakova
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Laboratory of Enzyme Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 159, Prospect 100-let Vladivostoku 690022, Russia.
authors.forename: Olesya S
authors.initials: OS
authors.lastname: Malyarenko Vishchuk
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Laboratory of Enzyme Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 159, Prospect 100-let Vladivostoku 690022, Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Isakov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Laboratory of Enzyme Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, Vladivostok, 159, Prospect 100-let Vladivostoku 690022, Russia.
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis; D000459:Phaeophyceae; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
chemical_list: D000464:Alginates; D000970:Antineoplastic Agents; D005936:Glucans; D006603:Hexuronic Acids; D009005:Monosaccharides; D011134:Polysaccharides; D020723:Glucuronic Acid; C008247:laminaran; C007789:fucoidan
keywords: Alginate; Anticancer activity; Cocophora langsdorfii; Fucoidan; Laminaran