title: Effects of Resveratrol on Receptor for Advanced Glycation End Products (RAGE) Expression and Oxidative Stress in the Liver of Rats with Type 2 Diabetes.
abstract: Incidence of diabetes mellitus is dramatically growing in the world. Oxidative stress, advanced glycation end products (AGEs) and receptor for AGE (RAGE) play key role in the pathogenesis of diabetes. Little is known about resveratrol effects on the liver. We hypothesize that resveratrol may exert a hepatoprotective effect in diabetic rats. Male rats with diabetes were treated with or without resveratrol at 1, 5 and 10 mg/kg body weight for 30 days. Total AGEs and malondialdehyde (MDA) levels in liver tissues were determined by spectrofluorimetric methods. Total antioxidant capacity and total oxidant contents in the liver and glucose in plasma were measured by a colorimetric assay. Expression of RAGE was assayed in liver of all animals using quantitative polymerase chain reaction. In liver tissue extract of resveratrol-treated rats with diabetes, MDA levels, total oxidant, plasma glucose and expression of RAGE were significantly reduced compared to the untreated group. Moreover, total antioxidant levels were significantly increased in treated rats. There was no significant difference in AGE contents among all groups. These results revealed that resveratrol had beneficial effects on the liver by extenuating oxidative stress and down regulation of RAGE expression.
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khazaei
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Karimi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Sheikh
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Goodarzi
authors.affiliation: Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Iraj
authors.initials: I
authors.lastname: Khodadadi
authors.affiliation: Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Heresh
authors.initials: H
authors.lastname: Moridi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D000067759:Receptor for Advanced Glycation End Products / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C000593874:Ager protein, rat; D000975:Antioxidants; D001786:Blood Glucose; D000067759:Receptor for Advanced Glycation End Products; D013267:Stilbenes; D008315:Malondialdehyde; D000077185:Resveratrol
keywords: RAGE; diabetes; liver; oxidative stress; resveratrol