title: Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro.
abstract: Hydrogen sulfide, one of three known gasotransmitters, is involved in physiological processes, including reproductive functions. Oocyte maturation and surrounding cumulus cell expansion play an essential role in female reproduction and subsequent embryonic development. Although the positive effects of exogenous hydrogen sulfide on maturing oocytes are well known, the role of endogenous hydrogen sulfide, which is physiologically released by enzymes, has not yet been described in oocytes. In this study, we observed the presence of Cystathionine β-Synthase (CBS), Cystathionine γ-Lyase (CTH) and 3-Mercaptopyruvate Sulfurtransferase (3-MPST), hydrogen sulfide-releasing enzymes, in porcine oocytes. Endogenous hydrogen sulfide production was detected in immature and matured oocytes as well as its requirement for meiotic maturation. Individual hydrogen sulfide-releasing enzymes seem to be capable of substituting for each other in hydrogen sulfide production. However, meiosis suppression by inhibition of all hydrogen sulfide-releasing enzymes is not irreversible and this effect is a result of M-Phase/Maturation Promoting Factor (MPF) and Mitogen-Activated Protein Kinase (MAPK) activity inhibition. Futhermore, cumulus expansion expressed by hyaluronic acid (HA) production is affected by the inhibition of hydrogen sulfide production. Moreover, quality changes of the expanded cumuli are indicated. These results demonstrate hydrogen sulfide involvement in oocyte maturation as well as cumulus expansion. As such, hydrogen sulfide appears to be an important cell messenger during mammalian oocyte meiosis and adequate cumulus expansion.
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic. Electronic address: nevoral@af.czu.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Nevoral
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Žalmanová
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Zámostná
authors.affiliation: Research Institute of Animal Production, Prague, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Kott
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kučerová-Chrpová
authors.affiliation: Université Lille1, Sciences et Technologies, Team Régulation des Signaux de Division, UMR 8576 CNRS, Villeneuve d'Ascq Cedex, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Bodart
authors.affiliation: Université Lille1, Sciences et Technologies, Team Régulation des Signaux de Division, UMR 8576 CNRS, Villeneuve d'Ascq Cedex, France.
authors.forename: Armance
authors.initials: A
authors.lastname: Gelaude
authors.affiliation: Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.
authors.forename: Radek
authors.initials: R
authors.lastname: Procházka
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Chemistry, Prague, Czech Republic.
authors.forename: Matyáš
authors.initials: M
authors.lastname: Orsák
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Chemistry, Prague, Czech Republic.
authors.forename: Miloslav
authors.initials: M
authors.lastname: Šulc
authors.affiliation: Charles University in Prague, Faculty of Medicine in Pilsen, Biomedical Centre, Pilsen, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Klein
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Dvořáková
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Weingartová
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Aurélia
authors.initials: A
authors.lastname: Víghová
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hošková
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Krejčová
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Jílek
authors.affiliation: Research Institute of Animal Production, Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Petr
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry; D006862:Hydrogen Sulfide / Q000378:metabolism*; D007150:Immunohistochemistry; D009865:Oocytes / Q000201:enzymology / Q000254:growth & development*; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine / Q000254:growth & development / Q000502:physiology*
chemical_list: D006820:Hyaluronic Acid; D006862:Hydrogen Sulfide
keywords: Cumulus expansion; GVBD; Gasotransmitter; Hydrogen sulfide; Meiotic maturation; Oocyte