title: Identification and characterization of a cytotoxic polysaccharide from the flower of Abelmoschus manihot.
abstract: A low molecular weight polysaccharide was identified from the flower of Abelmoschus manihot (Linn.) Medicus, a popular herbal medicine also known as Flos A. manihot. The polysaccharide, AMPS-a, was obtained from the ethanol-extracted debris of Flos A. manihot by successive purification through DEAE-cellulose-52 and Sephadex G-100 column. AMPS-a has an estimated molecular weight of 8.8×10(3)Da and is mainly composed of β-d-glucose, α-d-mannose, α-d-galactose and α-l-fucose at a molar ratio of 1.00:0.91:2.14:1.09. The preliminary structural features were studied by hydrolysis-methylation analysis, GC-MS, FT-IR and (1)H and (13)C NMR spectroscopy. AMPS-a contains a backbone composed of repeating units of →6)α-d-Galp-(1→6)α-d-Manp-(1→6)α-d-Galp-(1→ with β-d-Glcp (1→3)α-Fucp-(1→ branching at O-3 of mannose. AMPS-a exhibited potent inhibitory effects on the proliferation of hepatic (SMMC-7721, HepG2) and gastric (MGC-803, MKN-45) cancer cells in vitro, and the sugar branches were indispensible for the cytotoxicity. Our work contributes to the first report of a novel cytotoxic polysaccharide from Flos A. manihot that may provide a potential source of anti-tumor agent.
authors.affiliation: Department of Pharmacy, Nanjing University of Chinese Medicine Affiliated Hospital, Nanjing 210029, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Nanjing University of Chinese Medicine Affiliated Hospital, Nanjing 210029, PR China. Electronic address: liuzh1008@126.com.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Suqian First Hospital, Suqian 223800, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
authors.forename: Liling
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
authors.forename: Xingmiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Pharmacy, Nanjing University of Chinese Medicine Affiliated Hospital, Nanjing 210029, PR China. Electronic address: qfss1024@126.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Qian
mesh_terms: D028483:Abelmoschus / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides
keywords: Cytotoxicity; Flos Abelmoschus manihot; Polysaccharide; Structural features