title: proBDNF Attenuates Hippocampal Neurogenesis and Induces Learning and Memory Deficits in Aged Mice.
abstract: Mature brain-derived neurotrophic factor has shown promotive effect on neural cells in rodents, including neural proliferation, differentiation, survival, and synaptic formation. Conversely, the precursor of brain-derived neurotrophic factor (proBDNF) has been emerging as a differing protein against its mature form, for its critical role in aging process and neurodegenerative diseases. In the present study, we investigated the role of proBDNF in neurogenesis in the hippocampal dentate gyrus of aged mice and examined the changes in mice learning and memory functions. The results showed that the newborn cells in the hippocampus revealed a significant decline in proBDNF-treated group compared with bovine serum albumin group, but an elevated level in anti-proBDNF group. During the maturation period, no significant change was observed in the proportions of phenotype of the newborn cells among the three groups. In water maze, proBDNF-treated mice had poorer scores in place navigation test and probe test, compared with those from any other group. Thus, we conclude that proBDNF attenuates neurogenesis in the hippocampus and induces the deficits in learning and memory functions of aged mice.
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.|Department of Neurology, PLA 123 Hospital, 1052 Yanshan Road, Yuhui District, Bengbu, 233000, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medicine, Third Military Medical University, Chongqing, 400042, China.
authors.forename: Cheng-Ren
authors.initials: CR
authors.lastname: Li
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Jiao
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China.
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Neurology, Daping Hospital, Third Military Medical University, 10 Changjiang Branch Road, Yuzhong District, Chongqing, 400042, China. xzq881@163.com.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xu
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000633:toxicity*; D001973:Bromodeoxyuridine / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000187:drug effects*; D007859:Learning Disabilities / Q000139:chemically induced*; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced*; D051379:Mice; D008810:Mice, Inbred C57BL; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D011498:Protein Precursors / Q000633:toxicity*; D013550:Swimming / Q000523:psychology; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C499047:Mbp protein, mouse; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; C426674:brain-derived neurotrophic factor precursor; D001973:Bromodeoxyuridine
keywords: Cognitive impairment; Morris water maze; Neurodegenerative disease; Neurogenesis; proBDNF