title: Continuous synthesis of hexanal by immobilized hydroperoxide lyase in packed-bed reactor.
abstract: This study aimed to develop an optimal continuous procedure of immobilized hydroperoxide lyase (HPL)-catalyzed synthesis of hexanal. A central composite design was used to study the combined effect of substrate concentration and the residence time of the reactant on hexanal concentration. The optimum conditions for hexanal synthesis included a 13-HPOD concentration of 43.54 mM and a residence time of 60.99 min. The maximum hexanal concentration was 3560 ± 130 mg/L when 16 U of immobilized HPLwas used. Furthermore, the stability of immobilized HPL was significantly improved in the packed-bed reactor, as evidenced by the slowed enzyme inactivation and prolonged operation time. The immobilized HPL remained activity until 40 mL substrate solution flowed past the packed-bed reactor. The catalyst productivity of hexanal in the packed-bed reactor was 5.35 ± 0.34 mg/U, much higher than that in the batch stirred reactor. This study was greatly meaningful for providing a green method to the large-scale production of hexanal.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yufei
authors.initials: Y
authors.lastname: Hua
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism*; D000447:Aldehydes / Q000138:chemical synthesis*; D019149:Bioreactors; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry
chemical_list: D000447:Aldehydes; D004800:Enzymes, Immobilized; D003577:Cytochrome P-450 Enzyme System; C010463:n-hexanal; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase