title: Determination of Angiotensin-(1-7) with HPLC/Fluorescence-Detection.
abstract: Angiotensin-(1-7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it is now under investigation in combination with antioxidants as a reperfusion solution. The combination showed impressive effects on isolated hearts of male Wistar rats after induced ischemia. In this work a high performance liquid chromatography method with fluorescence detection was developed for the first time for in-process measurements as well as for stability tests of the peptide in the novel antioxidant-containing Karal® solution. For fluorescence detection of angiotensin-(1-7) fluorescamine as derivatization dye was applied. Under optimized conditions the method showed linearity over the range of 50 to 5000 ng/mL with R(2) of 0.9988 and an overall precision better than 5.0 %. LOD and LOQ were determined to be in the femtomol range on column. It was found that stability of angiotensin-(1-7) could be significantly improved in the antioxidant containing preparation compared to aqueous solutions.
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, 8010, Graz, Austria.
authors.forename: Miriam
authors.initials: M
authors.lastname: Russ
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, 8010, Graz, Austria.
authors.forename: Susanne
authors.initials: S
authors.lastname: Hauser
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, 8010, Graz, Austria.
authors.forename: Reinhold
authors.initials: R
authors.lastname: Wintersteiger
authors.affiliation: Institute of Physiological Chemistry, Medical University Graz, Harrachgasse 21, 8010, Graz, Austria.
authors.forename: Joachim
authors.initials: J
authors.lastname: Greilberger
authors.affiliation: Division of Transplantation Surgery, Medical University Graz, Auenbruggerplatz 29, 8036, Graz, Austria.
authors.forename: Michaela
authors.initials: M
authors.lastname: Andrä
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Schubertstraße 1, 8010, Graz, Austria. astrid.ortner@uni-graz.at.
authors.forename: Astrid
authors.initials: A
authors.lastname: Ortner
mesh_terms: D000803:Angiotensin I / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D008297:Male; D008856:Microscopy, Fluorescence; D008968:Molecular Conformation; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010446:Peptide Fragments; D000803:Angiotensin I; C118790:angiotensin I (1-7)
keywords: Angiotensin-(1–7); Fluorescamine; Fluorescence detection; HPLC; Stability