title: Cellulose Acetate Membrane Electrophoresis Based Urinary Proteomics for the Identification of Characteristic Proteins.
abstract: BACKGROUND
  Analysis of urinary proteins using cellulose acetate membrane electrophoresis (CAME) is a useful and challenging method for the recognition of damaged sites in the kidney. However, protein content of each CAME fraction is still not completely understood.
  
  
  METHODS
  In this study, an effective method of protein extraction from each band fractionated by CAME was established, which enabled us to examine the extracted proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry.
  
  
  RESULTS
  Proteins were extracted from the gel and analyzed by mass spectrometry. In all, 31 proteins were identified, including 20 urinary proteins that were newly identified in the CAME-based analysis.
  
  
  CONCLUSIONS
  This methodology was useful for identifying the proteins responsible for creating unique bands on CAME in a urine sample of a patient with drug-induced interstitial nephritis. These findings provide in-depth characterization of urinary protein contents in each CAME fraction.
authors.affiliation: Faculty of Health Science Technology, Bunkyo Gakuin University, Tokyo, Japan. anak@bgu.ac.jp.
authors.forename: Aki
authors.initials: A
authors.lastname: Nakayama
authors.affiliation: Department of Health Sciences, Saitama Prefectural University, Saitama, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kubota
authors.affiliation: Division of Clinical Nephrology and Rheumatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Sakatsume
authors.affiliation: Department of Pharmacology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Katayama
authors.affiliation: Faculty of Health Science Technology, Bunkyo Gakuin University, Tokyo, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Kanamori
authors.affiliation: Division of Health Care Science, Graduate School of Health Care Science, Bunkyo Gakuin University, Tokyo, Japan.
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Shiba
authors.affiliation: Faculty of Health Science Technology, Bunkyo Gakuin University, Tokyo, Japan.|Division of Health Care Science, Graduate School of Health Care Science, Bunkyo Gakuin University, Tokyo, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Iijima
mesh_terms: D001706:Biopsy; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D009395:Nephritis, Interstitial / Q000652:urine*; D011507:Proteinuria / Q000652:urine*; D020543:Proteome / Q000378:metabolism*
chemical_list: D020543:Proteome
keywords: SDS-PAGE; cellulose acetate membrane electrophoresis; kidney; proteome; urinary protein