title: High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool.
abstract: In wheat, a lack of genetic diversity between breeding lines has been recognized as a significant block to future yield increases. Species belonging to bread wheat's secondary and tertiary gene pools harbour a much greater level of genetic variability, and are an important source of genes to broaden its genetic base. Introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat, but this approach has been hampered by a lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphisms that can be used to genotype hexaploid wheat and to identify and track introgressions from a variety of sources. We have validated these markers using an ultra-high-density Axiom(®) genotyping array to characterize a range of diploid, tetraploid and hexaploid wheat accessions and wheat relatives. To facilitate the use of these, both the markers and the associated sequence and genotype information have been made available through an interactive web site.
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Mark O
authors.initials: MO
authors.lastname: Winfield
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Allen
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Burridge
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Gary L A
authors.initials: GL
authors.lastname: Barker
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Harriet R
authors.initials: HR
authors.lastname: Benbow
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Wilkinson
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Jane
authors.initials: J
authors.lastname: Coghill
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Christy
authors.initials: C
authors.lastname: Waterfall
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Davassi
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Geoff
authors.initials: G
authors.lastname: Scopes
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Ali
authors.initials: A
authors.lastname: Pirani
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Teresa
authors.initials: T
authors.lastname: Webster
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Fiona
authors.initials: F
authors.lastname: Brew
authors.affiliation: Affymetrix UK Ltd, High Wycombe, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Bloor
authors.affiliation: School of Biosciences, Sutton Bonington, Leicestershire, UK.
authors.forename: Julie
authors.initials: J
authors.lastname: King
authors.affiliation: John Innes Centre, Norwich Research Park, Norwich, Norfolk, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: West
authors.affiliation: John Innes Centre, Norwich Research Park, Norwich, Norfolk, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Griffiths
authors.affiliation: School of Biosciences, Sutton Bonington, Leicestershire, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: King
authors.affiliation: The John Bingham Laboratory, NIAB, Cambridge, UK.
authors.forename: Alison R
authors.initials: AR
authors.lastname: Bentley
authors.affiliation: Life Sciences, University of Bristol, Bristol, UK.
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Edwards
mesh_terms: D001947:Breeding; D005788:Gene Pool; D005819:Genetic Markers; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D060005:Genotyping Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: genotyping array; next-generation sequencing; secondary and tertiary gene pools; single nucleotide polymorphism; wheat; wheat progenitors