title: OsGF14b Positively Regulates Panicle Blast Resistance but Negatively Regulates Leaf Blast Resistance in Rice.
abstract: Although 14-3-3 proteins have been reported to be involved in responses to biotic stresses in plants, their functions in rice blast, the most destructive disease in rice, are largely unknown. Only GF14e has been confirmed to negatively regulate leaf blast. We report that GF14b is highly expressed in seedlings and panicles during blast infection. Rice plants overexpressing GF14b show enhanced resistance to panicle blast but are susceptible to leaf blast. In contrast, GF14b-silenced plants show increased susceptibility to panicle blast but enhanced resistance to leaf blast. Yeast one-hybrid assays demonstrate that WRKY71 binds to the promoter of GF14b and modulates its expression. Overexpression of GF14b induces expression of jasmonic acid (JA) synthesis-related genes but suppresses expression of salicylic acid (SA) synthesis-related genes. In contrast, suppressed GF14b expression causes decreased expression of JA synthesis-related genes but activation of SA synthesis-related genes. These results suggest that GF14b positively regulates panicle blast resistance but negatively regulates leaf blast resistance, and that GF14b-mediated disease resistance is associated with the JA- and SA-dependent pathway. The different functions for 14-3-3 proteins in leaf and panicle blast provide new evidence that leaf and panicle blast resistance are controlled by different mechanisms.
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: 3 Guangdong Key Laboratory of New Technology in Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences;
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Yang
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Shaohong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Junliang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: 3 Guangdong Key Laboratory of New Technology in Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences;
authors.forename: Aiqing
authors.initials: A
authors.lastname: Feng
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Tifeng
authors.initials: T
authors.lastname: Yang
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Xinxue
authors.initials: X
authors.lastname: Mao
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Jingfang
authors.initials: J
authors.lastname: Dong
authors.affiliation: 3 Guangdong Key Laboratory of New Technology in Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences;
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: 4 Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines; and.
authors.forename: Hei
authors.initials: H
authors.lastname: Leung
authors.affiliation: 5 Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80537-1177, U.S.A.
authors.forename: Jan E
authors.initials: JE
authors.lastname: Leach
authors.affiliation: 1 Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou 510640, China;|2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D020868:Gene Silencing; D020022:Genetic Predisposition to Disease; D012275:Oryza / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D020156:Salicylic Acid