title: Studying the Conformation of a Silaffin-Derived Pentalysine Peptide Embedded in Bioinspired Silica using Solution and Dynamic Nuclear Polarization Magic-Angle Spinning NMR.
abstract: Smart materials are created in nature at interfaces between biomolecules and solid materials. The ability to probe the structure of functional peptides that engineer biogenic materials at this heterogeneous setting can be facilitated tremendously by use of DNP-enhanced solid-state NMR spectroscopy. This sensitive NMR technique allows simple and quick measurements, often without the need for isotope enrichment. Here, it is used to characterize a pentalysine peptide, derived from a diatom's silaffin protein. The peptide accelerates the formation of bioinspired silica and gets embedded inside the material as it is formed. Two-dimensional DNP MAS NMR of the silica-bound peptide and solution NMR of the free peptide are used to derive its secondary structure in the two states and to pinpoint some subtle conformational changes that the peptide undergoes in order to adapt to the silica environment. In addition, interactions between abundant lysine residues and silica surface are identified, and proximity of other side chains to silica and to neighboring peptide molecules is discussed.
authors.affiliation: Department of Chemistry, Bar Ilan University , Ramat Gan 52900, Israel.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Geiger
authors.affiliation: Department of Chemistry, Bar Ilan University , Ramat Gan 52900, Israel.
authors.forename: Hugo E
authors.initials: HE
authors.lastname: Gottlieb
authors.affiliation: Leibniz Institute für Molekulare Pharmakologie (FMP), NMR Supported Structural Biology , Robert-Roessle-Str. 10, Berlin 13125, Germany.
authors.forename: Ümit
authors.initials: Ü
authors.lastname: Akbey
authors.affiliation: Leibniz Institute für Molekulare Pharmakologie (FMP), NMR Supported Structural Biology , Robert-Roessle-Str. 10, Berlin 13125, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
authors.affiliation: Department of Chemistry, Bar Ilan University , Ramat Gan 52900, Israel.
authors.forename: Gil
authors.initials: G
authors.lastname: Goobes