title: Role of TM4SF1 in regulating breast cancer cell migration and apoptosis through PI3K/AKT/mTOR pathway.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of transmembrane-4-l-six-family-1 (TM4SF1) on breast cancer cell line MDA-MB-231 invasion and apoptosis and its mechanism through PI3K/AKT/mTOR pathway.
  
  
  METHODS
  siRNA-TM4SF1 and pcDNA-TM4SF1 plasma were constructed and then transfected into MDA-MB-231 cells respectively. Real time (RT)-PCR was used to measure the mRNA expression of TM4SF1 in each group. Also, matrigel method and Annexin V-FITC were used to detect the effect of TM4SF1 expression on MDA-MB-231 cell migration and apoptosis respectively. Besides, western blotting analyze was used to assay the effects of TM4SF1 expression on PI3K/AKT/mTOR pathway associated proteins expressions.
  
  
  RESULTS
  The results showed that after being transfected with siRNA-TM4SF1, TM4SF1 expression was significantly declined, while it was significantly increased after cells were transfected with pcDNA-TM4SF1 (P<0.05). Compared with the controls, TM4SF1 overexpression significantly contributed MDA-MB-231 cell migration but decreased apoptotic cells (P<0.05), which were opposite to the results when TM4SF1 was sliced in cells. Moreover, TM4SF1 slicing significantly decreased the expressions of phosphorylated (p)-AKT, p-mTOR, and p-P70 (P<0.05).
  
  
  CONCLUSIONS
  Our study suggested that TM4SF1 may be a therapeutic target for breast cancer treatment and may loan insight into the mechanisms behind the development and metastasis of advanced breast cancer.
authors.affiliation: Department of General Surgery, No. 2 People's Hospital of Wuxi Jiangsu, 214000, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of General Surgery, No. 2 People's Hospital of Wuxi Jiangsu, 214000, China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of General Surgery, No. 2 People's Hospital of Wuxi Jiangsu, 214000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of General Surgery, No. 2 People's Hospital of Wuxi Jiangsu, 214000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.affiliation: Department of General Surgery, No. 2 People's Hospital of Wuxi Jiangsu, 214000, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wang
mesh_terms: D000954:Antigens, Surface / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014162:Transfection
chemical_list: D000954:Antigens, Surface; D009363:Neoplasm Proteins; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C074075:TM4SF1 protein, human
keywords: Breast cancer; PI3K/AKT/mTOR pathway; cell apoptosis; cell migration; transmembrane-4-l-six-family-1 (TM4SF1)