title: Identification of Specific Variations in a Non-Motile Strain of Cyanobacterium Synechocystis sp. PCC 6803 Originated from ATCC 27184 by Whole Genome Resequencing.
abstract: Cyanobacterium Synechocystis sp. PCC 6803 is a widely used model organism in basic research and biofuel biotechnology application. Here, we report the genomic sequence of chromosome and seven plasmids of a glucose-tolerant, non-motile strain originated from ATCC 27184, GT-G, in use at Guangzhou. Through high-throughput genome re-sequencing and verification by Sanger sequencing, eight novel variants were identified in its chromosome and plasmids. The eight novel variants, especially the five non-silent mutations might have interesting effects on the phenotype of GT-G strains, for example the truncated Sll1895 and Slr0322 protein. These resequencing data provide background information for further research and application based on the GT-G strain and also provide evidence to study the evolution and divergence of Synechocystis 6803 globally.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510641 Guangzhou, China. dingql21@126.com.
authors.forename: Qinglong
authors.initials: Q
authors.lastname: Ding
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510641 Guangzhou, China. chengu@scut.edu.cn.
authors.forename: Gu
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510641 Guangzhou, China. wang.yulingwyl@163.com.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510641 Guangzhou, China. fewd304@scut.edu.cn.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D046939:Synechocystis / Q000145:classification* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial
keywords: Synechocystis sp. PCC 6803; deletion; insertion; single nucleotide polymorphism; whole genome resequencing