title: Evaluation of Objective Vitritis Grading Method Using Optical Coherence Tomography: Influence of Phakic Status and Previous Vitrectomy.
abstract: OBJECTIVE
  To evaluate a proposed method for objective measurement of vitreous inflammation using a spectral-domain optical coherence tomography (SD OCT) device in a large cohort of uveitis eyes, including pseudophakic eyes and vitrectomized eyes.
  
  
  METHODS
  Retrospective, observational cohort study.
  
  
  METHODS
  One hundred five uveitis eyes (105 patients) with different vitreous haze score grades according to standardized protocols and corresponding SD OCT images (Cirrus HD-OCT; Carl Zeiss Meditec, Dublin, California, USA) were included. Clinical data recorded included phakic status, previous vitreoretinal surgery, and anterior chamber (AC) cells and flare. SD OCT images were analyzed using custom software that provided absolute measurements of vitreous (VIT) and retinal pigment epithelium (RPE) signal intensities, which were compared to generate a relative optical density ratio with arbitrary units (VIT/RPE-relative intensity) and compared to VHS.
  
  
  RESULTS
  VIT/RPE-relative intensity showed a significant positive correlation with vitreous haze score (r = 0.535, P < .001) that remained significant after adjusting for factors governing media clarity, such as AC cells, AC flare, and phakic status (R(2)-adjusted = 0.424, P < .001). Significant differences were also observed between the different vitreous haze score groups (P < .001). Preliminary observation did not observe differences in VIT/RPE-relative intensity values between phakic and pseudophakic eyes (0.3522 vs 0.3577, P = .48) and between nonvitrectomized and vitrectomized eyes (0.3540 vs 0.3580, P = .52), overall and respectively for each vitreous haze score subgroup.
  
  
  CONCLUSIONS
  VIT/RPE-relative intensity values provide objective measurements of vitreous inflammation employing an SD OCT device. Phakic status and previous vitrectomy surgery do not appear to influence these values, although these preliminary findings need validation in future studies.
authors.affiliation: Institut Clínic d'Oftalmologia, Hospital Clinic, Barcelona, Spain; National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom; School of Clinical Sciences, Bristol Eye Hospital, University of Bristol, Bristol, United Kingdom. Electronic address: jzarranz@hotmail.com.
authors.forename: Javier
authors.initials: J
authors.lastname: Zarranz-Ventura
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom.
authors.forename: Pearse A
authors.initials: PA
authors.lastname: Keane
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom.
authors.forename: Dawn A
authors.initials: DA
authors.lastname: Sim
authors.affiliation: Institut Clínic d'Oftalmologia, Hospital Clinic, Barcelona, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: Llorens
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom.
authors.forename: Adnan
authors.initials: A
authors.lastname: Tufail
authors.affiliation: Doheny Eye Institute, University of California Los Angeles, Los Angeles, California.
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Sadda
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom; School of Clinical Sciences, Bristol Eye Hospital, University of Bristol, Bristol, United Kingdom.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Dick
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom; School of Clinical Sciences, Bristol Eye Hospital, University of Bristol, Bristol, United Kingdom.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Lee
authors.affiliation: National Institute for Health Research Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital National Health Service Foundation Trust and University College London Institute of Ophthalmology, London, United Kingdom.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pavesio
authors.affiliation: Queen Elizabeth Hospital Birmingham, University Hospitals Birmingham National Health Service Foundation Trust, Birmingham, United Kingdom; Academic Unit of Ophthalmology, University of Birmingham, Birmingham, United Kingdom; Birmingham & Midland Eye Centre, Sandwell and West Birmingham National Health Service Trust, Birmingham, United Kingdom.
authors.forename: Alastair K
authors.initials: AK
authors.lastname: Denniston
authors.affiliation: Institut Clínic d'Oftalmologia, Hospital Clinic, Barcelona, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Adan
mesh_terms: D000328:Adult; D000867:Anterior Chamber / Q000473:pathology; D005128:Eye Diseases / Q000145:classification* / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000145:classification / Q000175:diagnosis; D007908:Lens, Crystalline / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D019591:Pseudophakia / Q000503:physiopathology; D055213:Retinal Pigment Epithelium / Q000473:pathology; D012189:Retrospective Studies; D041623:Tomography, Optical Coherence / Q000379:methods*; D014605:Uveitis / Q000150:complications*; D014792:Visual Acuity; D014821:Vitrectomy; D014822:Vitreous Body / Q000473:pathology*