title: The Sertoli cell hormones inhibin-B and anti Müllerian hormone have different patterns of secretion in prepubertal cryptorchid boys.
abstract: The Sertoli-cells produce inhibin-B and Anti-Müllerian-Hormone (AMH). Much is still unknown about these hormones in prepubertal cryptorchids. The Sertoli-cells are mandatory for germ cell development. The aim of the study was to investigate if there are differences in secretion pattern of Sertoli-cell hormones and their gonadotropin feed-back mechanisms.
  
  
  METHODS
  Included were 94 prepubertal cryptorchid boys 0.5-13.1years with measurements of serum-inhibin-B, Anti-Müllerian-Hormone (AMH), Luteinizing Hormone (LH) and Follicle Stimulation Hormone (FSH). The serum values were measured using commercially available kits. The hormonal values were related to age-matched normal values. Testicular biopsy was taken at orchiopexy.
  
  
  RESULTS
  Inhibin-B positively correlated to AMH for 1-13year-old patients (p<0.0001), but not for 0.5-1year-old patients (p=0.439). For 0.5-1year-old patients inhibin-B-values tended to decrease (p=0.055), in contrast to AMH-values (p=0.852). LH was elevated more often than FSH (p=0.014). FSH and LH were positively associated in patients both 0.5-1year (p=0.042) and 1-13years of age (p<0.0001). LH correlated positively to inhibin- B (p=0.001). In contrast, FSH did not correlate to inhibin-B or AMH (p=0.755 and p=0.528). The number of A-dark spermatogonia per tubular transverse section was positively correlated to inhibin-B serum level.
  
  
  CONCLUSIONS
  Our new finding of an association between LH and inhibin-B in infancy of cryptorchid boys may be essential for the transformation of gonocytes to A-dark spermatogonia. Previously, LH associated to inhibin-B was described in early puberty only. During the first year of life inhibin-B values decreased faster than AMH. The AMH-levels may just reflect the increased Sertoli cell number that occurs during the first 3months of life.
authors.affiliation: The Department of Pediatric Surgery, Rigshospitalet, DK-2100 Copenhagen; Section of Endocrinology, Department of Pediatrics, Hvidovre University Hospital, Copenhagen; Faculty of Health Science, University of Copenhagen.
authors.forename: Dina
authors.initials: D
authors.lastname: Cortes
authors.affiliation: Department of Pathology, Rigshospitalet, Copenhagen.
authors.forename: Erik
authors.initials: E
authors.lastname: Clasen-Linde
authors.affiliation: Douglas Stephens Surgical Research Laboratory, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Paediatric Urology, Royal Children's Hospital, Melbourne, Australia; Department of Paediatrics, University of Melbourne.
authors.forename: John M
authors.initials: JM
authors.lastname: Hutson
authors.affiliation: Douglas Stephens Surgical Research Laboratory, Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: Ruili
authors.initials: R
authors.lastname: Li
authors.affiliation: The Department of Pediatric Surgery, Rigshospitalet, DK-2100 Copenhagen; Faculty of Health Science, University of Copenhagen. Electronic address: Jorgen.Mogens.Thorup@regionh.dk.
authors.forename: Jorgen
authors.initials: J
authors.lastname: Thorup
mesh_terms: D000293:Adolescent; D000367:Age Factors; D054304:Anti-Mullerian Hormone / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003456:Cryptorchidism / Q000378:metabolism* / Q000601:surgery; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006801:Humans; D007223:Infant; D007265:Inhibins / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D056126:Orchiopexy; D012708:Sertoli Cells / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C422364:inhibin B; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Anti-Müllerian hormone; Cryptorchidism; Follicle stimulation hormone; Inhibin-B; Luteinizing hormone; Sertoli cells