title: Probing the nitrite and nitric oxide reductase activity of cbb3 oxidase: resonance Raman detection of a six-coordinate ferrous heme-nitrosyl species in the binuclear b3/CuB center.
abstract: In this work we report the first spectroscopic evidence demonstrating that cbb3 oxidase catalyzes the reduction of nitrite to nitrous oxide under reducing anaerobic conditions. The reaction proceeds through the formation of a ferrous six-coordinate heme b3-nitrosyl species that has been characterized by resonance Raman spectroscopy.
authors.affiliation: University of Cyprus, Department of Chemistry, PO Box 20537, 1678 Nicosia, Cyprus. effiep@ucy.ac.cy.
authors.forename: Andreas
authors.initials: A
authors.lastname: Loullis
authors.forename: Eftychia
authors.initials: E
authors.lastname: Pinakoulaki
mesh_terms: D003300:Copper / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D009569:Nitric Oxide / Q000737:chemistry*; D009573:Nitrites / Q000737:chemistry*; D009609:Nitrous Oxide / Q000737:chemistry*; D010084:Oxidation-Reduction; D044223:Pseudomonas stutzeri / Q000201:enzymology; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: D009573:Nitrites; D009569:Nitric Oxide; D006418:Heme; D003300:Copper; D007501:Iron; C414443:cbb3 oxidase; D003576:Electron Transport Complex IV; D009609:Nitrous Oxide; D003545:Cysteine