title: Chimeric Pestivirus Experimental Vaccines.
abstract: Chimeric pestiviruses have shown great potential as marker vaccine candidates against pestiviral infections. Exemplarily, we describe here the construction and testing of the most promising classical swine fever vaccine candidate "CP7_E2alf" in detail. The description is focused on classical cloning technologies in combination with reverse genetics.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald, Insel Riems, 17493, Germany.
authors.forename: Ilona
authors.initials: I
authors.lastname: Reimann
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald, Insel Riems, 17493, Germany. sandra.blome@fli.bund.de.
authors.forename: Sandra
authors.initials: S
authors.lastname: Blome
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald, Insel Riems, 17493, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology* / Q000627:therapeutic use; D006691:Classical Swine Fever / Q000517:prevention & control* / Q000821:virology; D007273:Injections, Intramuscular; D010576:Pestivirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D013552:Swine; D014611:Vaccination / Q000379:methods* / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000276:immunology / Q000627:therapeutic use; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000914:Antibodies, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
keywords: CP7_E2alf; Chimeric pestivirus; Cloning; Construction; Marker vaccine candidate; Reverse genetics