title: Screening of the chemical composition and bioactivity of Waldheimia glabra (Decne.) Regel essential oil.
abstract: BACKGROUND
  This research aimed at improving knowledge as to the chemical composition and the antibacterial and anti-cancer activities of the essential oil of Waldheimia glabra, a wild plant from the Himalayan Mountains.
  
  
  RESULTS
  The results obtained by GC-MS showed that spathulenol, 9-tetradecenol, thujopsene, α-thujone, santolina alcohol and terpinen-4-ol were the main constituents of Waldheimia glabra essential oil. These results were confirmed by HS-SPME GC-MS analysis that also reported high amounts of artemisia alcohol and camphor. Disc diffusion assay suggested a mild antibacterial activity against both Escherichia coli and Staphylococcus aureus. Finally, a dose-response correlation was observed between Waldhemia glabra essential oil concentration and viability of human breast adenocarcinoma cells MDA-MB-231 and MCF-7.
  
  
  CONCLUSIONS
  Together with the GC-MS method, HS-SPME GC-MS proved to be a reliable technique to characterise the chemical composition of essential oil obtained from aromatic plants. Further studies will focus on W. glabra phytochemicals and their biological activity, in order to support traditional uses of the plant. © 2015 Society of Chemical Industry.
authors.affiliation: Centre for Applied Studies in the Sustainable Management and Protection of the Mountain Environment-Ge.S.Di.Mont, University of Milan, Via Morino 8, 25048, Edolo, Brescia, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Manzo
authors.affiliation: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Loana
authors.initials: L
authors.lastname: Musso
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Panseri
authors.affiliation: Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, University of Milan, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iriti
authors.affiliation: Department of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Dallavalle
authors.affiliation: Department of Health Sciences, University of Piemonte Orientale 'A. Avogadro', Via Solaroli 17, 28100, Novara, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Catalano
authors.affiliation: Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Scarì
authors.affiliation: Centre for Applied Studies in the Sustainable Management and Protection of the Mountain Environment-Ge.S.Di.Mont, University of Milan, Via Morino 8, 25048, Edolo, Brescia, Italy.|Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, University of Milan, Via Celoria 2, 20133, Milan, Italy.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Giorgi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019306:Artemisia / Q000737:chemistry; D019659:Asteraceae / Q000737:chemistry*; D000081008:Bicyclic Monoterpenes; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013729:Terpenes / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C034019:terpinenol-4; C013258:spathulenol; C005790:beta-thujone; C433908:thujopsene
keywords: Waldheimia glabra (Decne.) Regel; anti-cancer activity; antibacterial activity; essential oils