title: Effects of fed-batch and continuous fermentations on human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors.
abstract: Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. This study was undertaken to evaluate fed-batch and continuous fermentations for the human lysozyme production in biofilm reactor. Results showed that addition of lactose the mid-log phase to make the concentration back to the initial level generates higher lysozyme production (177 U/ml) compared with lactose addition in late-log phase (174 U/ml) (p < 0.05). Moreover, fed-batch fermentation with glucose as initial carbon source and continuous addition of lactose with 0.6 ml/min for 10 h demonstrated significantly higher lysozyme production (187 U/ml) compared to the batch fermentation (173 U/ml) (p < 0.05). In continuous fermentation, biofilm reactor provided significantly higher productivity (7.5 U/ml/h) compared to the maximum productivity in suspended cell bioreactor (4 U/ml/h), because the biofilm reactor provided higher cell density at higher dilution rate compared to suspended cell reactor (p < 0.05).
authors.affiliation: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
authors.forename: Duygu
authors.initials: D
authors.lastname: Ercan
authors.affiliation: Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. demirci@psu.edu.|The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, PA, 16802, USA. demirci@psu.edu.
authors.forename: Ali
authors.initials: A
authors.lastname: Demirci
mesh_terms: D018441:Biofilms; D018533:Biomass; D019149:Bioreactors; D005285:Fermentation; D006801:Humans; D007716:Kluyveromyces / Q000378:metabolism*; D009113:Muramidase / Q000096:biosynthesis*
chemical_list: D009113:Muramidase
keywords: Biofilm reactor; Continuous; Fed-batch; Human lysozyme; Plastic composite support; Suspended cell reactor