title: Analytical fingerprint and chemometrics as phytochemical composition control tools in food supplement analysis: characterization of raspberry bud preparations of different cultivars.
abstract: BACKGROUND
  The raspberry, Rubus idaeus L., provides several plant parts (as buds) used for food supplements. The aim of this research was to establish a technique for chemical composition control of R. idaeus herbal preparations, using chromatographic methods. These methods allowed us to identify and quantify the main phytochemicals, obtaining a specific phytochemical fingerprint (phytocomplex). Combined with two different chemometric methods - clustering analysis and principal component analysis - the raspberry bud extracts of the different cultivars were efficiently characterized.
  
  
  RESULTS
  Rubus idaeus buds were identified as a rich source of anti-inflammatory and antioxidant compounds: organic acids, vitamins and catechins were found to be the most discriminating variables by chemometric techniques to differentiate raspberry cultivars. In particular, catechins (13.25%) and flavonols (8.71%) were the most important polyphenolic classes, followed by cinnamic and benzoic acids.
  
  
  CONCLUSIONS
  This study developed a useful tool for R. idaeus extract phytochemical characterization that could be applied also for differentiation and composition control of other herbal preparations. © 2015 Society of Chemical Industry.
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, 10095, Grugliasco, (TO), Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Donno
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, 10095, Grugliasco, (TO), Italy.
authors.forename: Gabriele L
authors.initials: GL
authors.lastname: Beccaro
authors.affiliation: Agroscope, Institute for Plant Production Sciences, 1964, Conthey, Switzerland.
authors.forename: Christoph
authors.initials: C
authors.lastname: Carlen
authors.affiliation: Agroscope, Institute for Plant Production Sciences, 1964, Conthey, Switzerland.
authors.forename: André
authors.initials: A
authors.lastname: Ançay
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, 10095, Grugliasco, (TO), Italy.
authors.forename: Alessandro K
authors.initials: AK
authors.lastname: Cerutti
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, 10095, Grugliasco, (TO), Italy.
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Mellano
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, Università degli Studi di Torino, 10095, Grugliasco, (TO), Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Bounous
mesh_terms: D000143:Acids / Q000032:analysis; D000893:Anti-Inflammatory Agents / Q000032:analysis; D000975:Antioxidants / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002845:Chromatography / Q000379:methods; D019587:Dietary Supplements / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D028321:Plant Preparations / Q000032:analysis*; D065856:Rubus / Q000737:chemistry*; D014815:Vitamins / Q000032:analysis
chemical_list: D000143:Acids; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts; D028321:Plant Preparations; D014815:Vitamins; D002392:Catechin
keywords: HPLC; Rubus idaeus; bioactive compounds; chemometrics; traceability