title: Activity of cholinesterases, pyruvate kinase and adenosine deaminase in rats experimentally infected by Fasciola hepatica: Influences of these enzymes on inflammatory response and pathological findings.
abstract: The aim of this study was to investigate acetylcholinesterase (AChE) in total blood and liver tissue; butyrylcholinesterase (BChE) in serum and liver tissue; adenosine deaminase (ADA) in serum and liver tissue; and pyruvate kinase (PK) in liver tissue of rats experimentally infected by Fasciola hepatica. Animals were divided into two groups with 12 animals each, as follows: group A (uninfected) and group B (infected). Samples were collected at 20 (A1 and B1;n=6 each) and 150 (A2 and B2; n=6 each) days post-infection (PI). Infected animals showed an increase in AChE activity in whole blood and a decrease in AChE activity in liver homogenates (P<0.05) at 20 and 150 days PI. BChE and PK activities were decreased (P<0.05) in serum and liver homogenates of infected animals at 150 days PI. ADA activity was decreased in serum at 20 and 150 days PI, while in liver homogenates it was only decreased at 150 days PI (P<0.05). Aspartate aminotransferase and alanine aminotransferase activities in serum were increased (P<0.05), while concentrations of total protein and albumin were decreased (P<0.05) when compared to control. The histological analysis revealed fibrous perihepatitis and necrosis. Therefore, we conclude that the liver fluke is associated with cholinergic and purinergic dysfunctions, which in turn may influence the pathogenesis of the disease.
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Ricardo E
authors.initials: RE
authors.lastname: Mendes
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Claiton I
authors.initials: CI
authors.lastname: Schwertz
authors.affiliation: Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil.
authors.forename: Neuber J
authors.initials: NJ
authors.lastname: Lucca
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Diessica
authors.initials: D
authors.lastname: Dalenogare
authors.affiliation: Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Guilherme V
authors.initials: GV
authors.lastname: Bochi
authors.affiliation: Department of Clinical and Toxicological Analysis, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Rafael N
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Maria R C
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: Laboratory of Nanosciences, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
authors.forename: Virginia C
authors.initials: VC
authors.lastname: Rech
authors.affiliation: Laboraroty of General Biochemistry and Microorganisms, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Jeandre A
authors.initials: JA
authors.lastname: Jaques
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil. Electronic address: aleksandro_ss@yahoo.com.br.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism*; D000818:Animals; D002802:Cholinesterases / Q000378:metabolism*; D004195:Disease Models, Animal; D005210:Fasciola hepatica; D005211:Fascioliasis / Q000201:enzymology* / Q000473:pathology*; D007249:Inflammation / Q000201:enzymology / Q000473:pathology; D008297:Male; D011770:Pyruvate Kinase / Q000378:metabolism*; D051381:Rats; D013053:Spectrophotometry
chemical_list: D011770:Pyruvate Kinase; D002802:Cholinesterases; D000243:Adenosine Deaminase
keywords: AChE; ADA; BChE; Fasciola hepatica; Hepatic function; PK