title: Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis.
abstract: ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans.
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ikeda
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kajiwara
authors.affiliation: Lipid Biology Laboratory and RIKEN, Saitama, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Iwamoto
authors.affiliation: Lipid Biology Laboratory and RIKEN, Saitama, Japan.
authors.forename: Asami
authors.initials: A
authors.lastname: Makino
authors.affiliation: Lipid Biology Laboratory and RIKEN, Saitama, Japan.
authors.forename: Toshihide
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuta
authors.affiliation: Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Funato
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005816:Genetic Complementation Test; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis*; D006706:Homeostasis; D006801:Humans; D050356:Lipid Metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C467736:ARV1 protein, S cerevisiae; C553399:ARV1 protein, human; D002352:Carrier Proteins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: GPI anchor; arv1Δ mutant; complementation; human ARV1; yeast