title: Proteomic analysis of a segregant population reveals candidate proteins linked to mealiness in peach.
abstract: Peaches are stored at low temperatures to delay ripening and increase postharvest life. However some varieties are susceptible to chilling injury,which leads to fruit mealiness, browning and flesh bleeding. In order to identify potentialmarkers associated with chilling injury,we performed proteomic analyses on a segregating population with contrasting susceptibility to chilling-induced mealiness. Chilling-induced mealiness was assessed by measuring juiciness in fruits that have been stored in cold and then allowed to ripen. Fruitmesocarp and leaf proteome from contrasting segregants were analyzed using 2-DE gels. Comparison of protein abundance between segregants revealed 133 spots from fruit mesocarp and 36 from leaf. Thirty four fruit mesocarp proteins were identified from these spots. Most of these proteins were related to ethylene synthesis, ABA response and stress response. Leaf protein analyses identified 22 proteins, most of which related to energy metabolism. Some of the genes that code for these proteins have been previously correlated with chilling injury through transcript analyses and co-segregation with mealiness QTLs. The results from this study, further deciphers the molecular mechanisms associated with chilling response in peach fruit, and identifies candidate proteins linked to mealiness in peach which may be used as putative markers for this trait.
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile. Electronic address: amiyasaka@unab.cl.
authors.forename: Andréa Miyasaka
authors.initials: AM
authors.lastname: Almeida
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Urra
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile.
authors.forename: Carol
authors.initials: C
authors.lastname: Moraga
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile.
authors.forename: Marcela
authors.initials: M
authors.lastname: Jego
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Flores
authors.affiliation: INTA-Universidad de Chile, Santiago, Chile.
authors.forename: Lee
authors.initials: L
authors.lastname: Meisel
authors.affiliation: FONDAP-Center of Genome Regulation (CGR), Santiago, Chile; INTA-Universidad de Chile, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: González
authors.affiliation: Departamento de Producción Agrícola, Universidad de Chile, Casilla, 1004 Santiago, Chile.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Infante
authors.affiliation: Instituto de Investigaciones Agropecuarias, INIA-La Platina, Santa Rosa 11610, Santiago, Chile.
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Defilippi
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile.
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Campos-Vargas
authors.affiliation: Universidad Andrés Bello, Fac. Ciencias Biológicas, Centro de Biotecnología Vegetal, República 217, Santiago, Chile; FONDAP-Center of Genome Regulation (CGR), Santiago, Chile.
authors.forename: Ariel
authors.initials: A
authors.lastname: Orellana
mesh_terms: D057773:Cold Shock Proteins and Peptides / Q000378:metabolism*; D058639:Cold-Shock Response / Q000502:physiology*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D000068239:Prunus persica / Q000378:metabolism*
chemical_list: D057773:Cold Shock Proteins and Peptides; D010940:Plant Proteins; D020543:Proteome
keywords: Chilling injury; Cold; Molecular markers; Postharvest; Proteome