title: Transcriptomic and Proteomic Data Integration and Two-Dimensional Molecular Maps with Regulatory and Functional Linkages: Application to Cell Proliferation and Invasion Networks in Glioblastoma.
abstract: Glioblastoma multiforme (GBM), the most aggressive primary brain tumor, is characterized by high rates of cell proliferation, migration, and invasion. New therapeutic strategies and targets are being continuously explored with the hope for better outcome. By overlaying transcriptomic and proteomic data from GBM clinical tissues, we identified 317 differentially expressed proteins to be concordant with the messenger RNAs (mRNAs). We used these entities to generate integrated regulatory information at the level of microRNAs (miRNAs) and their mRNA and protein targets using prediction programs or experimentally verified miRNA target mode in the miRWalk database. We observed 60% or even more of the miRNA-target pairs to be consistent with experimentally observed inverse expression of these molecules in GBM. The integrated view of these regulatory cascades in the contexts of cell proliferation and invasion networks revealed two-dimensional molecular interactions with regulatory and functional linkages (miRNAs and their mRNA-protein targets in one dimension; multiple miRNAs associated in a functional network in the second dimension). A total of 28 of the 35 differentially expressed concordant mRNA-protein entities represented in the proliferation network, and 51 of the 59 such entities represented in the invasion network, mapped to altered miRNAs from GBM and conformed to an inverse relationship in their expression. We believe the two-dimensional maps of gene expression changes enhance the strength of the discovery datasets derived from omics-based studies for their applications in GBM as well as tumors in general.
authors.affiliation: Institute of Bioinformatics , International Tech Park, Bangalore 560066, India.|Manipal University , Madhav Nagar, Manipal 576104, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Gupta
authors.affiliation: Institute of Bioinformatics , International Tech Park, Bangalore 560066, India.|Manipal University , Madhav Nagar, Manipal 576104, India.
authors.forename: Savita
authors.initials: S
authors.lastname: Jayaram
authors.affiliation: Neuro-Oncology, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Narayana Health , Bangalore 560099, India.
authors.forename: Divijendra Natha
authors.initials: DN
authors.lastname: Reddy
authors.affiliation: Institute of Bioinformatics , International Tech Park, Bangalore 560066, India.
authors.forename: Ravindra Varma
authors.initials: RV
authors.lastname: Polisetty
authors.affiliation: Institute of Bioinformatics , International Tech Park, Bangalore 560066, India.|Neuro-Oncology, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Narayana Health , Bangalore 560099, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sirdeshmukh
mesh_terms: D049109:Cell Proliferation; D016543:Central Nervous System Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D011506:Proteins; D012333:RNA, Messenger
keywords: 2D molecular maps; cell invasion; cell proliferation; glioblastoma; microRNA; proteomics; transcriptomics