title: Mouse superkiller-2-like helicase DDX60 is dispensable for type I IFN induction and immunity to multiple viruses.
abstract: IFN-α/β allow cells to fight virus infection by inducing the expression of many genes that encode effectors of antiviral defense. One of these, the Ski2-like DExH-box helicase DDX60, was recently implicated in resistance of human cells to hepatitis C virus, as well as in induction of IFN-α/β by retinoic acid inducible gene 1-like receptors (RLRs) that detect the presence of RNA viruses in a cell-intrinsic manner. Here, we sought to investigate the role of DDX60 in IFN-α/β induction and in resistance to virus infection. Analysis of fibroblasts and myeloid cells from Ddx60-deficient mice revealed no impairment in IFN-α/β production in response to RLR agonists, RNA viruses, or other stimuli. Moreover, overexpression of DDX60 did not potentiate IFN induction and DDX60 did not interact with RLRs or capture RLR agonists from virally infected cells. We also failed to identify any impairment in Ddx60-deficient murine cells or mice in resistance to infection with influenza A virus, encephalomyocarditis virus, Sindbis virus, vaccinia virus, or herpes simplex virus-1. These results put in question the reported role of DDX60 as a broad-acting positive regulator of RLR responses and hint at the possibility that it may function as a restriction factor highly specific for a particular virus or class of viruses.
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Delphine
authors.initials: D
authors.lastname: Goubau
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Annemarthe G
authors.initials: AG
authors.lastname: van der Veen
authors.affiliation: Bioinformatics, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London, UK.
authors.forename: Probir
authors.initials: P
authors.lastname: Chakravarty
authors.affiliation: Molecular Oncology Group, Lady Davis Institute-Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
authors.forename: Rongtuan
authors.initials: R
authors.lastname: Lin
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Rogers
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Jan
authors.initials: J
authors.lastname: Rehwinkel
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Safia
authors.initials: S
authors.lastname: Deddouche
authors.affiliation: Transgenic Services, The Francis Crick Institute, Clare Hall Laboratory, Potters Bar, Herts, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: Rosewell
authors.affiliation: Vaccine & Gene Therapy Institute of Florida, Port Saint Lucie, FL, USA.
authors.forename: John
authors.initials: J
authors.lastname: Hiscott
authors.affiliation: Immunobiology Laboratory, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, UK.
authors.forename: Caetano
authors.initials: C
authors.lastname: Reis e Sousa
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D053487:DEAD-box RNA Helicases / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis*; D051379:Mice; D051193:Toll-Like Receptors / Q000502:physiology; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D016207:Cytokines; D007370:Interferon Type I; D051193:Toll-Like Receptors; C561889:DDX60 protein, mouse; D053487:DEAD-box RNA Helicases
keywords: DDX60 ⋅ Innate immunity ⋅ Interferon ⋅ RIG-I-like helicases