title: MicroRNA-506 suppresses tumor proliferation and metastasis in colon cancer by directly targeting the oncogene EZH2.
abstract: Increasing evidence reveals that aberrant expression of microRNA contributes to the development and progression of colon cancer, but the roles of microRNA-506 (miR-506) in colon cancer remain elusive. Here, we demonstrated that miR-506 was down-regulated in colon cancer tissue and cells and that miR-506 expression was inversely correlated with EZH2 expression, tumor size, lymph node invasion, TNM stage and metastasis. A high level of miR-506 identified patients with a favorable prognosis. In vitro and in vivo experiments confirmed that miR-506 inhibits the proliferation and metastasis of colon cancer, and a luciferase reporter assay confirmed that EZH2 is a direct and functional target of miR-506 via the 3'UTR of EZH2. The restoration of EZH2 expression partially reversed the proliferation and invasion of miR-506-overexpressing colon cancer cells. Moreover, we confirmed that the miR-506-EZH2 axis inhibits proliferation and metastasis by activating/suppressing specific downstream tumor-associated genes and the Wnt/β-catenin signaling pathway. Taking together, our study sheds light on the role of miR-506 as a suppressor for tumor growth and metastasis and raises the intriguing possibility that miR-506 may serve as a new potential marker for monitoring and treating colon cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, 410008, PR China.|Department of Oncological Surgery, Affiliated Hospital of Xuzhou Medical College, 221000, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, Third Xiangya Hospital, Central South University, 410008, PR China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Lin
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, 410008, PR China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Liao
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, 410008, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Gastrointestinal Surgery, Guizhou Provincial People's Hospital, 550000, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Zhenran
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Xingsi
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Yangchao
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology / Q000628:therapy; D000071221:Enhancer of Zeste Homolog 2 Protein; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D063151:Polycomb Repressive Complex 2 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D013997:Time Factors; D014162:Transfection; D016896:Treatment Outcome; D060449:Wnt Signaling Pathway
chemical_list: D014408:Biomarkers, Tumor; C561508:MIRN506 microRNA, human; D035683:MicroRNAs; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2
keywords: EZH2; colon cancer; metastasis; miR-506; proliferation