title: Purification, Visualization, and Molecular Signature of Neural Stem Cells.
abstract: Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1 as a novel marker for NSCs and show that it can be used along with Lewis-X (LeX) to yield a highly purified population of NSCs. Using time-lapse microscopy, we are able to follow NSCs forming neurospheres, allowing their visualization. Finally, using single-cell polymerase chain reaction (PCR), we determine the molecular signature of NSCs. The single-cell PCR data suggest that along with the Notch and Shh pathways, the Hippo pathway plays an important role in NSC activity.
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Yuan Hong
authors.initials: YH
authors.lastname: Yu
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Gunaseelan
authors.initials: G
authors.lastname: Narayanan
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Shvetha
authors.initials: S
authors.lastname: Sankaran
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Srinivas
authors.initials: S
authors.lastname: Ramasamy
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Shi Yu
authors.initials: SY
authors.lastname: Chan
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Shuping
authors.initials: S
authors.lastname: Lin
authors.affiliation: 2 Bioinformatics Laboratory , Singapore Immunology Network, Singapore, Singapore .
authors.forename: Jinmiao
authors.initials: J
authors.lastname: Chen
authors.affiliation: 2 Bioinformatics Laboratory , Singapore Immunology Network, Singapore, Singapore .
authors.forename: Henry
authors.initials: H
authors.lastname: Yang
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Hariharan
authors.initials: H
authors.lastname: Srivats
authors.affiliation: 1 Neural Stem Cell Laboratory, Institute of Medical Biology , Singapore, Singapore .
authors.forename: Sohail
authors.initials: S
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation; D002478:Cells, Cultured; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D015415:Biomarkers