title: Developmental fluoxetine and prenatal stress effects on serotonin, dopamine, and synaptophysin density in the PFC and hippocampus of offspring at weaning.
abstract: Selective serotonin reuptake inhibitor medication exposure during the perinatal period can have a long term impact in adult offspring on neuroplasticity and the serotonergic system, but the impact of these medications during early development is poorly understood. The aim of this study was to determine the effects of developmental exposure to the SSRI, fluoxetine, on the serotonergic system, dopaminergic system, and synaptophysin density in the prefrontal cortex and hippocampus, as well as number of immature neurons in the dentate gyrus, in juvenile rat offspring at weaning. To model aspects of maternal depression, prenatal restraint stress was used. Sprague-Dawley rat offspring were exposed to either prenatal stress and/or fluoxetine. Main findings show that developmental fluoxetine exposure to prenatally stressed offspring decreased 5-HT and 5-HIAA levels and altered the dopaminergic system in the hippocampus. Prenatal stress, regardless of fluoxetine, increased synaptophysin density in the PFC. This work indicates that early exposure to maternal stress and SSRI medication can alter brain monoamine levels and synaptophysin density in offspring at weaning.
authors.affiliation: Department of Biological Sciences, Ohio University, Athens, OH.
authors.forename: Mary
authors.initials: M
authors.lastname: Gemmel
authors.affiliation: School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.
authors.forename: Ine
authors.initials: I
authors.lastname: Rayen
authors.affiliation: Department of Biological Sciences, Ohio University, Athens, OH.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Lotus
authors.affiliation: School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.
authors.forename: Eva
authors.initials: E
authors.lastname: van Donkelaar
authors.affiliation: School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands.
authors.forename: Harry W
authors.initials: HW
authors.lastname: Steinbusch
authors.affiliation: Department of Biomedical Sciences, Ohio University, Athens, OH.
authors.forename: Sonsoles
authors.initials: S
authors.lastname: De Lacalle
authors.affiliation: Department of Pharmacology, Medical School, University of Athens, Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Kokras
authors.affiliation: Department of Pharmacology, Medical School, University of Athens, Athens, Greece.
authors.forename: Christina
authors.initials: C
authors.lastname: Dalla
authors.affiliation: Department of Biological Sciences, Ohio University, Athens, OH. j.pawluski@gmail.com.|School for Mental Health and Neuroscience, Maastricht University, Maastricht, Netherlands. j.pawluski@gmail.com.|University of Rennes 1, IRSET-INSERM U1085, Rennes Cedex, France. j.pawluski@gmail.com.
authors.forename: Jodi L
authors.initials: JL
authors.lastname: Pawluski
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005473:Fluoxetine / Q000009:adverse effects*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000009:adverse effects*; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism*; D016708:Synaptophysin / Q000187:drug effects / Q000378:metabolism*; D014886:Weaning
chemical_list: D004298:Dopamine; D005473:Fluoxetine; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D016708:Synaptophysin
keywords: SSRI; antidepressants; development; dopamine; lactation; maternal depression; neurogenesis; neurotransmitters; prenatal stress; rat; serotonin; weaning