title: [Establishment and evaluation of a novel mouse model of orthotopic colon cancer in the mesenteric triangle of the cecum].
abstract: OBJECTIVE
  To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum.
  
  
  METHODS
  Twenty SPF 8-week old BALB/c mice (male:female = 1:1) were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26. WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model of orthotopic colon cancer.
  
  
  RESULTS
  Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice.
  
  
  CONCLUSIONS
  The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.
authors.affiliation: Department of Hematology, Zhongda Hospital of Southeast University, Nanjing 210009, China.
authors.forename: Xiangfeng
authors.initials: X
authors.lastname: He
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Song
authors.initials: S
authors.lastname: Wen
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Sun
authors.forename: Guojie
authors.initials: G
authors.lastname: Ling
authors.forename: Kang
authors.initials: K
authors.lastname: Shen
authors.forename: Chunlei
authors.initials: C
authors.lastname: Peng
authors.affiliation: Email: bachen@seu.edu.cn.
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
authors.affiliation: Email: ywk_wjh@163.com.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000556:secondary; D000818:Animals; D002430:Cecal Neoplasms / Q000473:pathology*; D002432:Cecum; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005240:Feasibility Studies; D005260:Female; D008113:Liver Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation / Q000379:methods