title: Prevalence of canine coronavirus (CCoV) in dog in Japan: detection of CCoV RNA and retrospective serological analysis.
abstract: We collected rectal swabs from dogs in Japan during 2011 to 2014, and canine coronavirus (CCoV) nucleocapsid gene was detected by RT-PCR. The relationship between CCoV infection and the manifestation of diarrhea symptoms was investigated, and a correlation was noted (df=1, χ(2)=8.90, P<0.005). The types of CCoV detected in samples from CCoV-infected dogs were CCoV-I in 88.9% and CCoV-II in 7.4%, respectively. We retrospectively investigated the seroprevalence of CCoV-I in dogs in Japan during 1998 to 2006. The sera were tested with a neutralizing antibody test. In the absence of CCoV-I laboratory strain, we used feline coronavirus (FCoV)-I that shares high sequence homology in the S protein with CCoV-I. 77.7% of the sera were positive for neutralizing anti-FCoV-I antibodies.
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.forename: Saya
authors.initials: S
authors.lastname: Yamashita
authors.forename: Michiko
authors.initials: M
authors.lastname: Murata-Ohkubo
authors.forename: Kumi
authors.initials: K
authors.lastname: Satoh
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Doki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D018352:Coronavirus Infections / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D000086462:Coronavirus Nucleocapsid Proteins; D017939:Coronavirus, Canine / Q000235:genetics; D004283:Dog Diseases / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D019590:Nucleocapsid Proteins / Q000235:genetics; D012367:RNA, Viral; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012189:Retrospective Studies
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral