title: Resveratrol induces ordered domains formation in biomembranes: Implication for its pleiotropic action.
abstract: Resveratrol is a polyphenol compound with great value in cancer therapy, cardiovascular protection, and neurodegenerative disorders. The mechanism by which resveratrol exerts such pleiotropic effects is not yet clear and there is a huge need to understand the influence of this compound on the regulation of lipid domains formation on membrane structure. The aim of the present study was to reveal potential molecular interactions between resveratrol and lipid rafts found in cell membranes by means of Förster resonance energy transfer, DPH fluorescence quenching, and triton X-100 detergent resistance assay. Liposomes composed of egg phosphatidylcholine, cholesterol, and sphingomyelin were used as model membranes. The results revealed that resveratrol induces phase separation and formation of liquid-ordered domains in bilayer structures. The formation of such tightly packed lipid rafts is important for different signal transduction pathways, through the regulation of membrane-associating proteins, that can justify several pharmacological activities of this compound.
authors.affiliation: UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Ana Rute
authors.initials: AR
authors.lastname: Neves
authors.affiliation: UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Nunes
authors.affiliation: UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal. Electronic address: shreis@ff.up.pt.
authors.forename: Salette
authors.initials: S
authors.lastname: Reis
mesh_terms: D000818:Animals; D002645:Chickens; D002784:Cholesterol / Q000737:chemistry; D003497:Cyclic N-Oxides / Q000737:chemistry; D004161:Diphenylhexatriene / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects*; D017830:Octoxynol / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D000077185:Resveratrol; D013109:Sphingomyelins / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D013267:Stilbenes; D004161:Diphenylhexatriene; D017830:Octoxynol; D002784:Cholesterol; D000077185:Resveratrol; C003959:TEMPO
keywords: Cholesterol; FRET; Lipid rafts; Liposomes; Sphingomyelin; Triton X-100