title: Collagen films with stabilized liquid crystalline phases and concerns on osteoblast behaviors.
abstract: To duplicate collagen's in vivo liquid crystalline (LC) phase and investigate the relationship between the morphology of LC collagen and osteoblast behavior, a self-assembly method was introduced for preparing collagen films with a stabilized LC phase. The LC texture and topological structure of the films before and after stabilization were observed with polarizing optical microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The relationship between the collagen films and osteoblast behavior was studied with the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide method, proliferation index detection, alkaline phosphatase measurements, osteocalcin assay, inverted microscopy, SEM observation, AFM observation, and cytoskeleton fluorescence staining. The results showed that the LC collagen film had continuously twisting orientations in the cholesteric phase with a typical series of arced patterns. The collagen fibers assembled in a well-organized orientation in the LC film. Compared to the non-LC film, the LC collagen film can promote cell proliferation, and increase ALP and osteocalcin expression, revealing a contact guide effect on osteoblasts.
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Min
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China. Electronic address: tjiaoyp@jnu.edu.cn.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Materials Science and Engineering, Jinan University, 510630, China. Electronic address: tcrz9@jnu.edu.cn.
authors.forename: Changren
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D050866:Liquid Crystals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024042:Collagen Type I; D015675:Osteocalcin; D000469:Alkaline Phosphatase
keywords: Collagen; Contact guide; Liquid crystal phase; Osteoblasts; Topological structure