title: Supplementation with difructose anhydride III promotes passive calcium absorption in the small intestine immediately after calving in dairy cows.
abstract: The incidence of hypocalcemia increases in high-parity dairy cows because resorption of bone Ca is delayed in these animals, and they appear to have a reduced ability to absorb Ca from the intestine during the early postpartum period. Difructose anhydride (DFA) III has been shown to promote the absorption of intestinal Ca via a paracellular pathway. However, past studies have not reported this effect in peripartum dairy cows. Therefore, we investigated the effect of DFA III supplementation on Ca metabolism during the peripartum period to determine whether DFA III promotes intestinal Ca absorption via this route. Seventy-four multiparous Holstein cows were separated into DFA and control groups based on their parity and body weight. The feed of the DFA group was supplemented with 40g/d of DFA III from -14 to 6d relative to calving. The control group did not receive DFA III. At calving (0h relative to calving), serum Ca declined below 9mg/dL in both groups. However, serum Ca concentrations were greater in the DFA group than in the control group at 6, 12, 24, and 48h relative to calving, and the time required for serum Ca to recover to 9mg/dL during the postpartum period was shorter in the high-parity cows in the DFA group than in those in the control group. Parathyroid hormone concentrations increased immediately after calving in both groups and were greater in the control group than in the DFA group at 12 and 24h relative to calving. Serum 1,25-dihydroxyvitamin D concentrations increased at 0 and 12h relative to calving in both groups and were higher in the control group than in the DFA group at 72h relative to calving. Serum concentrations of the bone-resorption marker cross-linked N-telopeptide of type I collagen (NTX) were not different between the groups during peripartum period, and serum NTX in all cows was lower at 0, 6, 12, 24, 48, and 72h relative to calving than at -21, 4, and 5d relative to calving. Thus, DFA treatment induced faster recovery of serum Ca, although bone resorption was restrained. In conclusion, DFA III promotes intestinal passive Ca absorption via the paracellular pathway during the early postpartum period; this absorption is unaffected by aging.
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, 020-8550, Japan; Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan. Electronic address: teramu@nitten.co.jp.
authors.forename: M
authors.initials: M
authors.lastname: Teramura
authors.affiliation: United Graduate School of Agricultural Sciences, Iwate University, Morioka, Iwate, 020-8550, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Wynn
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Reshalaitihan
authors.affiliation: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Kyuno
authors.affiliation: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.affiliation: Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Hokkaido, 080-0831, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohtani
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Kawashima
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hanada
mesh_terms: D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002136:Calcium, Dietary; D002417:Cattle / Q000502:physiology*; D024042:Collagen Type I / Q000097:blood; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004187:Disaccharides / Q000008:administration & dosage*; D005260:Female; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000378:metabolism; D010281:Parathyroid Hormone / Q000097:blood; D010455:Peptides / Q000097:blood; D058725:Peripartum Period / Q000502:physiology; D049590:Postpartum Period; D011247:Pregnancy
chemical_list: D002136:Calcium, Dietary; D024042:Collagen Type I; D004187:Disaccharides; D010281:Parathyroid Hormone; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; C087758:difructose anhydride III; D002118:Calcium
keywords: calcium; dairy cow; difructose anhydride (DFA) III; hypocalcemia; paracellular pathway