title: Protein-Protein Multilayer Oil-in-Water Emulsions for the Microencapsulation of Flaxseed Oil: Effect of Whey and Fish Gelatin Concentration.
abstract: The impact of whey protein isolate (WPI) and fish gelatin (FG) deposited sequentially at concentrations of 0.1, 0.5, and 0.75% on the surface of primary oil-in-water emulsions containing 5% flaxseed oil stabilized with either 0.5% fish gelatin or whey protein, respectively, was investigated. The results revealed that the adsorption of WPI/FG or FG/WPI complexes to the emulsion interface led to the formation of oil-in-water (o/w) emulsions with different stabilities and different protection degrees of the flaxseed oil. Deposition of FG on the WPI primary emulsion increased the particle size (from 0.53 to 1.58 μm) and viscosity and decreased electronegativity (from -23.91 to -11.15 mV) of the complexes. Different trends were noted with the deposition of WPI on the FG primary emulsion, resulting in decreasing particle size and increasing electronegativity and viscosity to a lower extent. Due to the superior tension-active property of WPI, the amount of protein load in the WPI primary emulsion as well as in WPI/FG complex was significantly higher than the FG counterparts. A multilayer emulsion made with 0.5% WPI/0.75% FG exhibited the lowest oxidation among all of the multilayered emulsions tested (0.32 ppm of hexanal) after 21 days, likely due to the charge effect of FG that may prevent pro-oxidant metals to interact with the flaxseed oil.
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fustier
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Allaoua
authors.initials: A
authors.lastname: Achouri
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Taherian
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Britten
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Marylène
authors.initials: M
authors.lastname: Pelletier
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Hassan
authors.initials: H
authors.lastname: Sabik
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Villeneuve
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Mondor
mesh_terms: D000818:Animals; D004339:Drug Compounding / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry; D029941:Fish Proteins / Q000737:chemistry*; D005399:Fishes; D005780:Gelatin / Q000737:chemistry*; D008043:Linseed Oil / Q000737:chemistry*; D010316:Particle Size; D014783:Viscosity; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D004655:Emulsions; D029941:Fish Proteins; D000067816:Whey Proteins; D008043:Linseed Oil; D005780:Gelatin
keywords: flax oil; gelatin; lipid oxidation; multilayered oil-in-water emulsions; whey protein