title: Anti-platelet effects of nimesulide in isoproterenol-induced myocardial ischaemia and infarction in rabbits.
abstract: BACKGROUND
  The present study was designed to determine the effects of cyclooxygenase-2 (COX-2) inhibitor-nimesulide on platelet activating factor (PAF) and arachidonic acid (AA)-induced platelet aggregation during myocardial infarction (Ml) and ischaemia in rabbits.
  
  
  METHODS
  Rabbits were divided into three groups; group I served as control and contains normal rabbits that received saline, group II rabbits received saline before induction of Ml, and group Ill rabbits received nimesulide (25 mg/kg) before Ml induction. Myocardial infarction was induced by isoproterenol (ISP) (65 mg/kg) and confirmed by biochemical, electrophysiological and histopathological methods. Platelet aggregation was induced with arachidonic acid (AA) and platelet activating factor (PAF), and monitored by using light transmission aggregometery.
  
  
  RESULTS
  When platelets were challenged with two different aggregating agents, nimesulide-treated infarcted rabbits showed resistance to platelet aggregation while non-treated infarcted rabbits were found prone to aggregation with both aggregating agents (P < 0.05).
  
  
  CONCLUSIONS
  These results suggest platelet inhibitory effects of nimesulide during experimental ischaemia and infarction in rabbits.
authors.forename: Sagheer
authors.initials: S
authors.lastname: Ahmed
authors.forename: Saima
authors.initials: S
authors.lastname: Gul
authors.forename: Hawa
authors.initials: H
authors.lastname: Ze Jaafar
authors.forename: Marius
authors.initials: M
authors.lastname: Moga
authors.forename: Muhammad
authors.initials: M
authors.lastname: Zia-Ul-Haq
authors.forename: Lorena
authors.initials: L
authors.lastname: Dima
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007545:Isoproterenol / Q000494:pharmacology*; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D010972:Platelet Activating Factor / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010979:Platelet Function Tests / Q000379:methods; D011817:Rabbits; D013449:Sulfonamides / Q000494:pharmacology*; D013566:Sympathomimetics / Q000494:pharmacology; D016896:Treatment Outcome
chemical_list: D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D013449:Sulfonamides; D013566:Sympathomimetics; D007545:Isoproterenol; C012655:nimesulide