title: A novel sensitive cell-based Love Wave biosensor for marine toxin detection.
abstract: A novel HepG2 cell-based biosensor using Love Wave sensor was developed to implement the real-time and sensitive detection of a diarrheic shellfish poisoning (DSP) toxin, Okadaic acid (OA). Detachable Love Wave sensor unit and miniaturized 8-channel recording instrument were designed for the convenient experimental preparation and sensor response signal measurement. The Love Wave sensor, whose synchronous frequency is around 160 MHz, was fabricated with ST-cut quartz substrate. To establish a cell-based biosensor, HepG2 cells as sensing elements were cultured onto the Love Wave sensor surface, and the cell attachment process was recorded by this biosensor. Results showed this sensor could monitor the cell attachment process in real time and response signals were related to the initial cell seeding densities. Furthermore, cell-based Love Wave sensor was treated with OA toxin. This biosensor presented a good performance to various OA concentrations, with a wide linear detection range (10-100 μg/L). Based on the ultrasensitive acoustic wave platform, this cell-based biosensor will be a promising tool for real-time and convenient OA screening.
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Jiaru
authors.initials: J
authors.lastname: Fang
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zou
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Yingchang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Lang
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Gao
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China. Electronic address: huning@zju.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Biosensor National Special Lab, Key Lab for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, 310027 Hangzhou, China. Electronic address: cnpwang@zju.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002448:Cell Adhesion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004867:Equipment Design; D019544:Equipment Failure Analysis; D056945:Hep G2 Cells; D006801:Humans; D008387:Marine Toxins / Q000032:analysis* / Q000633:toxicity; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D019319:Okadaic Acid / Q000032:analysis / Q000633:toxicity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018675:Toxicity Tests / Q000295:instrumentation*
chemical_list: D008387:Marine Toxins; D019319:Okadaic Acid
keywords: Cell attachment; Cell-based biosensor; Love Wave sensor; Okadaic acid (OA)