title: [EXPRESSIONS OF CARTILAGE DEGENERATIVE RELATED GENES AND microRNAs IN TORN MENISCUS].
abstract: OBJECTIVE
  To investigate the expressions of cartilage degenerative related genes in meniscus, and to evaluate the potential effect of meniscal damage on cartilage degeneration, and to analyze the relationship between microRNAs (miRNAs) expression and cartilage degeneration.
  
  
  METHODS
  Meniscal tissue was collected from 5 patients undergoing partial meniscectomy between September 2012 and October 2013 (experimental group), and normally meniscal tissue without tearing from amputees was used as controls (control group). Pathological changes of menisci were observed; and real-time fluorescent quatitative PCR was performed to examine the relative expression levels of cartilage degenerative related genes and miRNAs: Aggrecan (ACAN), type X collagen (COL10A1), matrix metalloproteinases 13 (MMP-13), CCAAT enhancer binding protein β (CEBP-β), a disintegrin and metalloproteinase with thrombospondinmotif 5 (ADAMTS-5), miR-193b, miR-92a, and miR-455-3p in meniscus.
  
  
  RESULTS
  There were varying degrees of degenerative pathological changes in torn meniscus of experimental group. Compared with normal meniscus of control group, the expression of ACAN was decreased, while the expressions of COL10A1, CEBP-β, ADAMTS-5, and MMP-13 were increased in torn meniscus of experimental group; and significant difference was found (P < 0.05) except ACAN and MMP-13 (P > 0.05). The expressions of miR-92a, miR-455-3p, and miR-193b in torn meniscus of experimental group were significantly higher than those in normal meniscus of control group (P < 0.05).
  
  
  CONCLUSIONS
  Meniscal tissue has the intrinsic tendency of degeration after meniscus tear. The torn meniscus has greater stimulative impact on cartilage degeneration than normally morphological meniscus without tearing. The cartilage degenerative related miRNAs, including miR-193b, miR-92a, and miR-455-3p may contribute to the up-regulation of osteoarthritis.
authors.forename: Yi
authors.initials: Y
authors.lastname: Long
authors.forename: Aishan
authors.initials: A
authors.lastname: He
authors.forename: Zhiqi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Fangang
authors.initials: F
authors.lastname: Meng
authors.forename: Changhe
authors.initials: C
authors.lastname: Hou
authors.forename: Ziji
authors.initials: Z
authors.lastname: Zhang
authors.forename: Guangxin
authors.initials: G
authors.lastname: Huang
authors.forename: Weiming
authors.initials: W
authors.lastname: Liao
mesh_terms: D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D024121:Collagen Type X; D005786:Gene Expression Regulation; D006801:Humans; D007270:Injections, Intra-Articular; D007718:Knee Injuries; D007719:Knee Joint; D008297:Male; D053509:Matrix Metalloproteinase 13; D008592:Menisci, Tibial / Q000187:drug effects* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010003:Osteoarthritis / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012421:Rupture; D000070600:Tibial Meniscus Injuries; D015854:Up-Regulation; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D053674:Aggrecans; D024121:Collagen Type X; D035683:MicroRNAs; D053509:Matrix Metalloproteinase 13