title: Metabolite Profile of Salidroside in Rats by Ultraperformance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry and High-Performance Liquid Chromatography Coupled with Quadrupole-Linear Ion Trap Mass Spectrometry.
abstract: In the present work, the salidroside metabolite profile in rat urine was investigated, and subsequently the metabolic pathways of salidroside were proposed. After administrations of salidroside at an oral dose of 100 or 500 mg/kg, rat urine samples were collected and pretreated with methanol to precipitate the proteins. The pretreated samples were analyzed by an Acquity ultraperformance liquid chromatography (UPLC) coupled with an HSS T3 column and detected by quadrupole time-of-flight mass spectrometry (Q-TOF-MS) or high-performance liquid chromatography coupled with hybrid triple-quadrupole linear ion trap mass spectrometry (HPLC/Q-trap-MS). A total of eight metabolites were detected and identified on the basis of the characteristics of their protonated ions in the urine samples. The results elucidated that salidroside was metabolized via glucuronidation, sulfation, deglycosylation, hydroxylation, methylation, and dehydroxylation pathways in vivo.
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.|Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150001, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Han
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Yan
authors.affiliation: Alkali Soil Natural Environmental Science Center, Northeast Forestry University , Harbin 150040, China.|Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education , Harbin 150040, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000652:urine*; D008297:Male; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000652:urine*; D051381:Rats; D017208:Rats, Wistar; D029771:Rhodiola / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010636:Phenols; C009172:rhodioloside
keywords: HPLC/Q-trap-MS; UPLC/Q-TOF-MS; metabolic pathways; metabolites; salidroside