title: Electrochemical Detection of Platinum(IV) Prodrug Satraplatin in Serum.
abstract: We report the design and fabrication of a reagentless and reusable electrochemical sensor for detection of satraplatin (SAT), a platinum(IV) prodrug. The detection strategy is based on the electrocatalytic reaction between the Pt(IV) center of SAT and surface-immobilized methylene blue. We systematically evaluated the effect of passivating diluent chain length on the overall sensor performance. Our results show that the use of a shorter diluent like 2-mercaptoethanol is more advantageous than using a longer and more passivating diluent such as 6-mercapto-1-hexanol. Independent of the use of cyclic voltammetry or chronoamperometry as the sensor interrogation technique, all three sensors, each passivated with a different alkanethiol diluent, have been demonstrated to be sensitive; the limit of detection is in the range of 1-10 μM. They are also highly specific and do not respond to Pt(II) drugs such as cisplatin and carboplatin. More importantly, they are selective enough to be employed directly in 50% serum. This sensing strategy has potential applications in clinical pharmacokinetics studies.
authors.affiliation: 651 Hamilton Hall, University of Nebraska-Lincoln , Lincoln, Nebraska 68588-0304, United States.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 651 Hamilton Hall, University of Nebraska-Lincoln , Lincoln, Nebraska 68588-0304, United States.
authors.forename: Rebecca Y
authors.initials: RY
authors.lastname: Lai
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D057230:Limit of Detection; D009944:Organoplatinum Compounds / Q000097:blood*; D011355:Prodrugs / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D011355:Prodrugs; C081294:satraplatin