title: Laminin therapy for the promotion of muscle regeneration.
abstract: Muscle regeneration is essentially due to activation of satellite cells, which can be isolated and amplified ex vivo, thus representing good candidates for cell therapy. Accumulating data show that the local microenvironment plays a major role during muscle regeneration. In the satellite cell niche, a major extracellular matrix protein is laminin. Human myoblasts transplanted into immunodeficient mice are preferentially located in laminin-enriched areas. Additionally, laminin-111 enhances myoblast proliferation in vitro and increases expression of the α7β1 integrin-type laminin receptor. Intramuscular injection of laminin-111 ameliorates muscular pathology in mdx mice, protecting muscle fibers from damage. Moreover, transplantation of human myoblasts with laminin-111 into Rag/mdx immunodeficient recipients improved efficacy of myoblast transplantation, increasing the number of human dystrophin-positive myofibres. Taken together, these data strongly indicate that exogenous laminin can ameliorate the regeneration process in different models of muscular dystrophies and can be instrumental for improving cell therapy aiming at repairing the degeneration/regeneration process in skeletal muscle.
authors.affiliation: Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Fiocruz/Inserm/UPMC International Associated Laboratory on Cell Therapy and Immunotherapy, Pierre and Marie Curie University, Sorbonne Universities, Paris, France.
authors.forename: Ingo
authors.initials: I
authors.lastname: Riederer
authors.affiliation: Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Fiocruz/Inserm/UPMC International Associated Laboratory on Cell Therapy and Immunotherapy, Pierre and Marie Curie University, Sorbonne Universities, Paris, France; Program on Cancer Research, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
authors.forename: Adriana Cesar
authors.initials: AC
authors.lastname: Bonomo
authors.affiliation: Fiocruz/Inserm/UPMC International Associated Laboratory on Cell Therapy and Immunotherapy, Pierre and Marie Curie University, Sorbonne Universities, Paris, France; Center of Research in Myology, Pierre and Marie Curie University, Sorbonne Universities, Paris, France; INSERM U974, Paris, France; CNRS-FRE 3617, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Mouly
authors.affiliation: Laboratory on Thymus Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; Fiocruz/Inserm/UPMC International Associated Laboratory on Cell Therapy and Immunotherapy, Pierre and Marie Curie University, Sorbonne Universities, Paris, France. Electronic address: savino@fiocruz.br.
authors.forename: Wilson
authors.initials: W
authors.lastname: Savino
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D006801:Humans; D007797:Laminin / Q000378:metabolism* / Q000494:pharmacology; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology; D012038:Regeneration / Q000187:drug effects
chemical_list: D007797:Laminin
keywords: Laminin; Laminin therapy; Muscle regeneration; Muscular disease; Myoblasts transplantation