title: [EFFECTS OF LEUCOCYTE- AND PLATELET-RICH PLASMA ON OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN TREATING AVASCULAR NECROSIS OF FEMORAL HEAD IN RABBITS].
abstract: OBJECTIVE
  To evaluate the effect of leucocyte- and platelet-rich plasma (L-PRP) on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in treating avascular necrosis of the femoral head (ANFH) in rabbits.
  
  
  METHODS
  Twenty-four New Zealand white rabbits (4-6 months old, both genders, weighing 2.0-3.0 kg) were used for the establishment of bilateral ANFH models and divided into 4 groups (n = 6). BMSCs were isolated from the bone marrow of iliac crest, cultured and identified. L-PRP was prepared by Landesberg method. Core decompression only (group A), core decompression and L-PRP implantation (group B), core decompression and BMSCs implantation (group C), and core decompression and implantation of BMSCs and L-PRP were performed in 4 groups. To evaluate bone formation and remodeling of the defects, X-ray photography was taken at 2, 4, and 8 weeks postoperatively. The modified Lane-Sandhu scoring system was used to evaluate the bone formation. Two rabbits were sacrificed at 2, 4, 8 weeks after operation to harvest the specimens for histological observation, new blood vessel count and new bone area ratio.
  
  
  RESULTS
  The observations of radiology and histology displayed different degrees of bone regeneration at bone defect sites in each group. At 2, 4, and 8 weeks postoperatively, the results of Lane-Sandhu X-ray photography scoring, new blood vessel count, and new bone area ratio showed that groups C and D were significantly better than groups A and B, group D was significantly better than group C. and group B was significantly better than group A (P < 0.05).
  
  
  CONCLUSIONS
  These findings demonstrate that L-PRP can promote osteogenic differentiation of BMSCs in treating ANFH in rabbits, and core decompression associated with BMSCs and L-PRP is an effective and feasible method to treat ANFH.
authors.forename: Jialiu
authors.initials: J
authors.lastname: Fang
authors.forename: Zongsheng
authors.initials: Z
authors.lastname: Yin
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Weilu
authors.initials: W
authors.lastname: Gao
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D001861:Bone Regeneration; D001842:Bone and Bones; D002454:Cell Differentiation; D002478:Cells, Cultured; D005271:Femur Head Necrosis / Q000628:therapy; D006412:Hematopoietic Stem Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D011817:Rabbits