title: Reporting a new siderophore based Ca(2+) selective chemosensor that works as a staining agent in the live organism Artemia.
abstract: A Ca(2+)-specific chemosensor involving acyclic non-ether and non-carboxylato-type metal chelating ligands is rare. The tetradentate OONO artificial receptor, HL, possessing a sulfur-containing intermediate siderophore aeruginic acid, tethered to a rhodamine 6G based signalling unit in a single molecule has been synthesized. The fluoroionophore required excitation in the visible wavelength (510 nm) and showed highly selective and sensitive detection of Ca(2+) ions in 100% water solution in HEPES buffer at physiological pH (7.4). The probe HL, with LOD as low as 70 nM, behaves reversibly and showed nearly 17-fold enhanced selectivity for Ca(2+) over other cell abundant alkali and alkaline metal ions such as Na(+), K(+), Li(+), and Mg(2+) without any intervention. Job's plot, (1)H NMR titration and ESI-MS data provided corroborative evidence in support of 1 : 1 association between HL and Ca(2+). From a wide range of transition and heavy metal ions series, HL also binds Cu(2+). However, the use of l-cysteine removes the interference from Cu(2+) and results in highly selective detection specificity of HL for Ca(2+). As a reversible "off-on-off" fluorescent chemosensor, it is possible to detect Ca(2+) at as low as 5 μM in the midgut region of the gastrointestinal tract of the live animal Artemia, a brine shrimp.
authors.affiliation: Analytical Discipline and Centralized Instrument Facility (AD&CIF), CSIR-CSMCRI, Bhavnagar, Gujarat, India. pbchatterjee@csmcri.org icpbc2008@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Raju
authors.affiliation: Analytical Discipline and Centralized Instrument Facility (AD&CIF), CSIR-CSMCRI, Bhavnagar, Gujarat, India. pbchatterjee@csmcri.org icpbc2008@gmail.com.
authors.forename: Ratish R
authors.initials: RR
authors.lastname: Nair
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-CSMCRI, Bhavnagar, Gujarat, India. shaldar@csmcri.org.
authors.forename: Ishan H
authors.initials: IH
authors.lastname: Raval
authors.affiliation: Marine Biotechnology and Ecology Division, CSIR-CSMCRI, Bhavnagar, Gujarat, India. shaldar@csmcri.org and Academy of Scientific and Innovative Research (AcSIR), CSIR-CSMCRI, Bhavnagar, Gujarat, India.
authors.forename: Soumya
authors.initials: S
authors.lastname: Haldar
authors.affiliation: Analytical Discipline and Centralized Instrument Facility (AD&CIF), CSIR-CSMCRI, Bhavnagar, Gujarat, India. pbchatterjee@csmcri.org icpbc2008@gmail.com and Academy of Scientific and Innovative Research (AcSIR), CSIR-CSMCRI, Bhavnagar, Gujarat, India.
authors.forename: Pabitra B
authors.initials: PB
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D001156:Artemia / Q000737:chemistry* / Q000648:ultrastructure; D002118:Calcium / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry; D002413:Cations, Divalent / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D061848:Optical Imaging; D012235:Rhodamines / Q000737:chemistry*; D017262:Siderophores / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D012235:Rhodamines; D017262:Siderophores; D013844:Thiazoles; C026188:rhodamine 6G; D002118:Calcium