title: Alternative Splicing in Next Generation Sequencing Data of Saccharomyces cerevisiae.
abstract: mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systematic analysis of mRNA sequencing data. We find evidence of a pervasive use of alternative splice sites and detect several novel introns both within and outside protein coding regions. We also find a predominance of alternative splicing on the 3' side of introns, a finding which is consistent with existing knowledge on conservation of exon-intron boundaries in S. cerevisiae. Some of the alternatively spliced transcripts allow for a translation into different protein products.
authors.affiliation: Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Schreiber
authors.affiliation: Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany.
authors.forename: Gergely
authors.initials: G
authors.lastname: Csaba
authors.affiliation: Department Chemie, Technische Universität München, Garching, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Haslbeck
authors.affiliation: Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Zimmer
mesh_terms: D017398:Alternative Splicing; D059014:High-Throughput Nucleotide Sequencing; D007438:Introns; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome