title: Ultra-sensitive immunosensor for detection of hepatitis B surface antigen using multi-functionalized gold nanoparticles.
abstract: The signal amplification for analytical purposes has considerable potential in detecting trace levels of analytes for clinical, security or environmental applications. In the present report a strategy based on a sandwich type immunoassay system was designed for the detection of hepatitis B surface antigen which exploits the specific affinity interaction between streptavidin and biotin recognition systems. The method involves the specific coupling of multi-functionalized gold nanoparticles (bearing biotin and luminol molecules) to the streptavidin modified by secondary antibody. The chemiluminescent signal is produced by the gold nanoparticles in the presence of HAuCl4 as catalyst and hydrogen peroxide as oxidant. The immunosensor was able to detect hepatitis B surface antigen in the linear concentration range from 1.7 to 1920 pg mL(-1) and the detection limit of 0.358 pg mL(-1), at signal/noise = 3.
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 131451384, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Shourian
authors.affiliation: Laboratory of Microanalysis, Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 131451384, Tehran, Iran; Nanobiomedicine Center of Excellence, Nanoscience and Nanotechnology Research Center, University of Tehran, Tehran, Iran. Electronic address: ghourchian@ut.ac.ir.
authors.forename: H
authors.initials: H
authors.lastname: Ghourchian
authors.affiliation: Pishtaz Teb Zaman Co., Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Boutorabi
mesh_terms: D002384:Catalysis; D002712:Chlorides / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D017612:Gold Compounds / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D002712:Chlorides; D017612:Gold Compounds; D006514:Hepatitis B Surface Antigens; D006046:Gold; C024568:gold tetrachloride, acid; D006861:Hydrogen Peroxide
keywords: Biotin; Chemiluminescence; Gold nanoparticles; Hepatitis B surface antigen; Immunoassay; Streptavidin