title: Engineered nanoconstructs for the multiplexed and sensitive detection of high-risk pathogens.
abstract: Many countries categorize the causative agents of severe infectious diseases as high-risk pathogens. Given their extreme infectivity and potential to be used as biological weapons, a rapid and sensitive method for detection of high-risk pathogens (e.g., Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Vaccinia virus) is highly desirable. Here, we report the construction of a novel detection platform comprising two units: (1) magnetic beads separately conjugated with multiple capturing antibodies against four different high-risk pathogens for simple and rapid isolation, and (2) genetically engineered apoferritin nanoparticles conjugated with multiple quantum dots and detection antibodies against four different high-risk pathogens for signal amplification. For each high-risk pathogen, we demonstrated at least 10-fold increase in sensitivity compared to traditional lateral flow devices that utilize enzyme-based detection methods. Multiplexed detection of high-risk pathogens in a sample was also successful by using the nanoconstructs harboring the dye molecules with fluorescence at different wavelengths. We ultimately envision the use of this novel nanoprobe detection platform in future applications that require highly sensitive on-site detection of high-risk pathogens.
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr.
authors.forename: Youngmin
authors.initials: Y
authors.lastname: Seo
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr.
authors.forename: Ji-eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr.
authors.forename: Yoon
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr and Department of Bionano Engineering, Hanyang University ERICA, Ansan 426-791, Korea.
authors.forename: Kwan Hong
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr.
authors.forename: Jangsun
authors.initials: J
authors.lastname: Hwang
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr and Department of Bionano Engineering, Hanyang University ERICA, Ansan 426-791, Korea.
authors.forename: Jongwook
authors.initials: J
authors.lastname: Hong
authors.affiliation: School of Integrative Engineering, Chung-Ang University, Seoul 156-756, Korea. jonghchoi@hanyang.ac.kr.
authors.forename: Hansoo
authors.initials: H
authors.lastname: Park
authors.affiliation: Department of Bionano Technology, Graduate School, Hanyang University, Seoul 133-791, Korea. heyshoo@cau.ac.kr and Department of Bionano Engineering, Hanyang University ERICA, Ansan 426-791, Korea.
authors.forename: Jonghoon
authors.initials: J
authors.lastname: Choi
mesh_terms: D001052:Apoferritins / Q000737:chemistry*; D001419:Bacteria; D045663:Quantum Dots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014616:Vaccinia virus
chemical_list: D001052:Apoferritins