title: Novel polydopamine imprinting layers coated magnetic carbon nanotubes for specific separation of lysozyme from egg white.
abstract: Novel core-shell nanocomposites, consisting of magnetic carbon nanotubes (MCNTs) core surrounded by a thin polydopamine (PDA) imprinting shell for specific recognition of lysozyme (Lyz), were fabricated for the first time. The obtained products were characterized and the results showed that the PDA layer was successfully attached onto the surface of MCNTs and the corresponding thickness of imprinting layer was just about 10nm which could enable the template access the recognition cavities easily. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results indicated that the obtained imprinted polymers showed fast kinetic and high affinity towards Lyz and could be used to specifically separate Lyz from real egg white. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption-regeneration cycles. Easy preparation, rapid separation, high binding capacity, and satisfactory selectivity for the template protein make this polymer attractive in biotechnology and biosensors.
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: ruixiagao@mail.xjtu.edu.cn.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Gao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Dechun
authors.initials: D
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an 710049, China; College of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, China. Electronic address: tyh57@mail.xjtu.edu.cn.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D004529:Egg White / Q000737:chemistry*; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D059346:Magnets / Q000737:chemistry*; D054802:Molecular Imprinting; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D007211:Indoles; D037742:Nanotubes, Carbon; D011108:Polymers; C568283:polydopamine; D009113:Muramidase
keywords: Lysozyme; Magnetic carbon nanotubes; Polydopamine; Surface imprinting