title: Persisters: Methods for Isolation and Identifying Contributing Factors--A Review.
abstract: Persister cells are phenotypic variants surviving a lethal dose of antibiotic, sufficient to kill the bulk of an exponential phase population. In this chapter we summarize current techniques to isolate persisters and discuss limitations associated with identifying mechanisms of persister formation.
authors.affiliation: Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Rowe
authors.affiliation: Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Conlon
authors.affiliation: Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA.
authors.forename: Iris
authors.initials: I
authors.lastname: Keren
authors.affiliation: Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA. k.lewis@neu.edu.
authors.forename: Kim
authors.initials: K
authors.lastname: Lewis
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007239:Infections / Q000188:drug therapy / Q000235:genetics / Q000382:microbiology*; D050296:Microbial Viability / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic; Biofilm; Hip mutant; Toxin-antitoxin (TA) modules