title: Label-free Quantitative Analysis of Changes in Broiler Liver Proteins under Heat Stress using SWATH-MS Technology.
abstract: High temperature is one of the key environmental stressors affecting broiler production efficiency and meat yield. Knowledge of broiler self-regulation mechanisms under heat stress is important for the modern scale of poultry breeding. In the present study, the SWATH strategy was employed to investigate the temporal response of the broiler liver to heat stress. A total of 4,271 proteins were identified and used to generate a reference library for SWATH analysis. During this analysis, 2,377 proteins were quantified, with a coefficient of variation ≤25% among technical and biological replicates. A total of 257 proteins showed differential expression between the control and heat stressed groups. Consistent results for 26 and 5 differential proteins were validated respectively by MRM and western blotting quantitative analyses. Bioinformatics analysis suggests that the up- and down-regulation of these proteins appear involved in the following three categories of cellular pathways and metabolisms: 1) inhibit the ERK signaling pathway; 2) affect broiler liver lipid and amino acid metabolism; 3) induce liver cell immune responses to adapt to the high temperatures and reduce mortality. The study reported here provides an insight into broiler self-regulation mechanisms and shed light on the improved broiler adaptability to high-temperature environments.
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Xiangfang
authors.initials: X
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Qingshi
authors.initials: Q
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: Institute of Biotechnology, Cornell University, Ithaca, NY 14853-2703, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Hongfu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Minhong
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D018869:Heat-Shock Response / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D020543:Proteome