title: Anticonvulsant and behavioral effects observed in mice following treatment with an ester derivative of ferulic acid: Isopentyl ferulate.
abstract: The objective of this study was to evaluate the potential anticonvulsant effect of isopentyl ferulate, a new ester derived from ferulic acid in mice (Mus musculus) subjected to two models of induced seizures. According to the results obtained, the IF at doses of 25, 50 and 75 mg/kg (i.p.) showed protective effect against induced seizures by pilocarpine (400 mg/kg, i.p.) and pentylenetetrazole (70 mg/kg, i.p.). In the two animal models of seizures, the pretreatment of the IF (25, 50 and 75 mg/kg) with flumazenil blocked the anticonvulsant effect, suggesting that the mechanism of action of this ester derived of ferulic acid may be related to activity in the benzodiazepine-binding site of the GABAA receptor (γ-aminobutyric acid, type A). In addition to the anticonvulsant effect, behavioral changes as neurotoxicity indication were assessed by using the rota rod and open field tests. The results obtained showed that the IF (25, 50 and 75 mg/kg) does not induce significant changes in locomotor activity and motor coordination when compared with the control group, unlike the results presented by diazepam. Thus, these results demonstrate a new pharmacological knowledge of IF with potential application against epileptic seizures. However, further studies are needed to elucidate other neurobiological mechanisms underlying epilepsy.
authors.affiliation: Post-Graduation Program in Pharmacology, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil.
authors.forename: Keylla C
authors.initials: KC
authors.lastname: Machado
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil.
authors.forename: George Laylson S
authors.initials: GL
authors.lastname: Oliveira
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil.
authors.forename: Kátia C
authors.initials: KC
authors.lastname: Machado
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil.
authors.forename: Md Torequl
authors.initials: MT
authors.lastname: Islam
authors.affiliation: Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil.
authors.forename: Antonio Luiz G
authors.initials: AL
authors.lastname: Junior
authors.affiliation: Department of Pharmaceutical Science, Federal University of Paraíba, CEP 58.051-900, João Pessoa, Paraíba, Brazil.
authors.forename: Damião P
authors.initials: DP
authors.lastname: De Sousa
authors.affiliation: Post-Graduation Program in Pharmacology, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil; Laboratory of Research in Experimental Neurochemistry of Post-Graduation Program in Pharmaceutical Sciences, Federal University of Piauí, CEP 64.049-550, Teresina, Piauí, Brazil. Electronic address: rivelilson@pq.cnpq.br.
authors.forename: Rivelilson M
authors.initials: RM
authors.lastname: Freitas
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D001522:Behavior, Animal / Q000187:drug effects*; D003373:Coumaric Acids / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D010433:Pentylenetetrazole / Q000009:adverse effects; D010862:Pilocarpine / Q000009:adverse effects; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology
chemical_list: D000927:Anticonvulsants; D003373:Coumaric Acids; C000607202:isopentyl ferulate; D010862:Pilocarpine; C004999:ferulic acid; D010433:Pentylenetetrazole
keywords: Anticonvulsant effect; Epileptic seizures; Isopentyl ferulate; Locomotor activity; Motor coordination