title: In Vitro Studies of Membrane Permeability Induced by Amyloidogenic Polypeptides Using Large Unilamellar Vesicles.
abstract: The process of amyloid formation is cytotoxic and contributes to a wide range of human diseases, but the mechanisms of amyloid-induced cytotoxicity are not well understood. It has been proposed that amyloidogenic peptides exert their toxic effects by damaging membranes. Membrane disruption is clearly not the only mechanism of toxicity, but the literature suggests that loss of membrane integrity may be a contributing factor. In this chapter we describe the measurement of in vitro membrane leakage induced by amyloidogenic proteins via the use of model vesicles. We use islet amyloid polypeptide (IAPP, amylin) as an example, but the methods are general.
authors.affiliation: Structural Biology Program, Kimmel Center for Biology and Medicine at the Skirball Institute, New York University School of Medicine, New York, NY, USA. Ping.Cao@med.nyu.edu.
authors.forename: Ping
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of Chemistry, Stony Brook University, 647 Chemistry, Stony Brook, NY, 11794-3400, USA. daniel.raleigh@stonybrook.edu.|Graduate Program in Biochemistry and Structural Biology, Stony Brook University, Stony Brook, NY, 11794-3400, USA. daniel.raleigh@stonybrook.edu.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Raleigh
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D058227:Amyloidogenic Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002463:Cell Membrane Permeability / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007515:Islets of Langerhans / Q000737:chemistry / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D058227:Amyloidogenic Proteins; D058228:Islet Amyloid Polypeptide; D053835:Unilamellar Liposomes
keywords: Amyloid; Cytotoxicity; Islet amyloid polypeptide; Membrane disruption; Membrane leakage