title: Endothelium adjustments to acute resistance exercise are intensity-dependent in healthy animals.
abstract: OBJECTIVE
  We evaluated the acute effects of different intensities of resistance exercise over endothelium-dependent vasodilatation, eNOSser1177 phosphorylation level and endothelial production of NO in superior mesenteric artery of healthy rats.
  
  
  METHODS
  Groups: control (Ct), resistance exercise in the intensities of 30% (Ex30%), 50% (Ex50%) and 70% (Ex70%) of the maximal load established by the maximal repetition test (1RM). Exercise protocol: 15 sets of 10 repetitions. The rings of mesenteric artery were mounted in an isometric system or were prepared for further implementation of Western blot and DAF-FM techniques.
  
  
  RESULTS
  The maximal response of the relaxation induced by insulin was not altered in the animals of the Ex30% group when compared to the Ct group. However, the animals of the Ex50% and Ex70% groups presented an increase in this response when compared to the Ct group. The eNOSser1177 phosphorylation levels showed an increase in Ex50% and Ex70% groups when compared to the Ct (1.6-fold and 3.3-fold, respectively). In the endothelial production of NO, it was observed that the Ex30% group did not show alteration in the NO production when compared to the Ct group. On the other hand, the animals exercised in the Ex50% and Ex70% groups showed increase in the NO synthesis when compared to the animals in the Ct group.
  
  
  CONCLUSIONS
  Our results suggest that the magnitude of these vascular endothelium adjustments is strongly related to the increase of the resistance exercise intensity from the intensity of 50% of 1 RM.
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Marcelo Mendonça
authors.initials: MM
authors.lastname: Mota
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Thássio Ricardo Ribeiro
authors.initials: TR
authors.lastname: Mesquita
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Tharciano Luiz Teixeira
authors.initials: TL
authors.lastname: Braga da Silva
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Milene Tavares
authors.initials: MT
authors.lastname: Fontes
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Sandra
authors.initials: S
authors.lastname: Lauton Santos
authors.affiliation: Department of Pharmacology, Federal University of Minas Gerais, Minas Gerais, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: dos Santos Aggum Capettini
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Itamar Couto
authors.initials: IC
authors.lastname: Guedes de Jesus
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans Júnior
authors.affiliation: Laboratory of Translational Physiology, Nove de Julho University, São Paulo, Brazil.
authors.forename: Kátia
authors.initials: K
authors.lastname: De Angelis
authors.affiliation: Department of Physical Education, Federal University of Sergipe, Sergipe, Brazil.
authors.forename: Rogério Brandão
authors.initials: RB
authors.lastname: Wichi
authors.affiliation: Department of Physiology, Federal University of Sergipe, Sergipe, Brazil. Electronic address: marcio@infonet.com.br.
authors.forename: Márcio Roberto Viana
authors.initials: MR
authors.lastname: Santos
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis*; D010766:Phosphorylation / Q000502:physiology; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation / Q000502:physiology*
chemical_list: D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
keywords: ENOS; Nitric oxide; Vasorelaxation