title: Biotin-conjugated fusogenic liposomes for high-quality cell purification.
abstract: Purification of defined cell populations from mixed primary cell sources is essential for many biomedical and biotechnological applications but often very difficult to accomplish due to missing specific surface markers. In this study, we developed a new approach for efficient cell population separation based on the specific membrane fusion characteristics of distinct cell types upon treatment with fusogenic liposomes. When such liposomes are conjugated with biotin, specific cell populations can be efficiently surface functionalized by biotin after liposomal treatment while other populations remain unlabeled. Due to the high affinity of biotin for avidin-like proteins, biotin functionalized cells are ideal targets for conjugation of e.g. avidin tagged magnetic beads, fluorophores or antibodies with bioanalytical relevance. Here, based on the differential biotinylation of distinct cell populations high quality separation of cardiac fibroblasts from myocytes, and cerebromicrovascular endothelial cells from fibroblasts was successfully established.
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Hersch
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Wolters
authors.affiliation: Reynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center, Oklahoma, USA.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Ungvari
authors.affiliation: Reynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center, Oklahoma, USA.
authors.forename: Tripti
authors.initials: T
authors.lastname: Gautam
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Dhruva
authors.initials: D
authors.lastname: Deshpande
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Merkel
authors.affiliation: Reynolds Oklahoma Center on Aging, Donald W. Reynolds Department of Geriatric Medicine, University of Oklahoma Health Sciences Center, Oklahoma, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Csiszar
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
authors.affiliation: Institute of Complex Systems, ICS-7: Biomechanics, Forschungszentrum Jülich GmbH, Jülich, Germany a.csiszar@fz-juelich.de.
authors.forename: Agnes
authors.initials: A
authors.lastname: Csiszár
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods; D001710:Biotin / Q000737:chemistry / Q000493:pharmacokinetics*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D018189:Immunomagnetic Separation / Q000379:methods*; D008081:Liposomes / Q000737:chemistry*; D008561:Membrane Fusion / Q000502:physiology*; D032342:Muscle Cells / Q000166:cytology*; D058726:Nanoconjugates / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008081:Liposomes; D058726:Nanoconjugates; D001710:Biotin
keywords: Plasma membrane functionalization; cell purification; cell separation; cell surface biotinylation; fusogenic liposomes