title: Pharmacokinetics of irisolidone and its main metabolites in rat plasma determined by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
abstract: Irisolidone, a major isoflavone found in Pueraria lobata flowers, exhibits a wide spectrum of bioactivities, while its metabolic pathways and the pharmacokinetics of its metabolites in vivo have not been investigated yet. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) method was employed to investigate the metabolic pathways of irisolidone and the pharmacokinetics of its main metabolites in rats, after a single 100mg/kg oral dose of irisolidone. Protein precipitation method was used to prepare plasma samples. A total of 15 metabolites included irisolidone were detected and tentatively identified based on the mass spectral fragmentation patterns, elution order or confirmed using available reference standards. The pharmacokinetics of the main metabolites included three glucuronide metabolites tectorigenin-7-O-glucuronide (Te-7G), 6-hydroxybiochanin A-6-O-glucuronide (6-OH-BiA-6G), irisolidone-7-O-glucuronide (Ir-7G), and three sulfate metabolite tectorigenin-7-O-sulfate-4'-O-sulfate (Te-7S-4'S), tectorigenin-7-O-sulfate (Te-7S) and irisolidone-7-O-sulfate (Ir-7S), and aglycone tectorigenin (Te), and irisolidone (Ir) were evaluated. The plasma concentrations reached maximal values of 0.297μmol/L at 10.3h for Te-7S-4'S, 0.199μmol/L at 21.7h for Te-7G, 0.154μmol/L at 8.00h for Te-7S, 4.10μmol/L at 15.3h for 6-OH-BiA-6G, 10.7μmol/L at 9.71h for Ir-7G, 0.918μmol/L at 11.3h for Te, 0.150μmol/L at 8.67h for Ir-7S, and 0.843μmol/L at 9.67h for Ir, respectively. Since the total plasma concentrations of conjugated metabolites were much higher than that of the irisolidone aglycone, an extensive phase II metabolism plays an important role in the pharmacokinetics of irisolidone in vivo.
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China; Department of Translational Medicine, Yancheng Institute of Health Scinces, 263 Jiefang Road, Yancheng 224005, China.
authors.forename: Guozhe
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Qi
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Liangyu
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kano
authors.affiliation: Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China. Electronic address: yuandan_kampo@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D016014:Linear Models; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005419:Flavonoids; C423902:irisolidone
keywords: Irisolidone; Metabolites; Plasma pharmacokinetics; Rat; UHPLC/Q-TOF MS