title: Anti-Inflammatory Constituents from Bidens frondosa.
abstract: A new polyacetylene glucoside (3E,5E,11E)-tridecatriene-7,9-diyne-1,2,13-triol-2-O-β-D-glucopyranoside (1), a new phenylpropanoid glucoside 2'-butoxyethylconiferin (2), and a new flavonoid glycoside 8,3',4'-trihydroxyflavone-7-O-(6''-O-p-coumaroyl)-β-D-glucopyranoside (3), have been isolated from Bidens frondosa together with fifty-three known compounds 4-56. The structures of these compounds were established by spectroscopic methods. mainly ESIMS, 1D- and 2D-NMR spectroscopic data. and comparison with literature data. Compounds 1-34, 36, 39, 43, 47, 51, and 52 were tested for inhibition of nuclear factor kappa B (NF-κB) in 293-NF-κB-luciferase report cell line induced by lipopolysaccharide (LPS), and compounds 1, 2, 3, 9, 15, 21, 24 and 51 were tested for the production of TNF-α, IL-1β, IL-6, IL-10 in RAW 264.7 macrophages induced by LPS. In conclusion, the isolated compounds 1, 2, 3, 9, 15, 21, 24 and 51 exhibited significant activity in anti-inflammatory activity assays.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. ycxxxj@163.com.|College of Chemical and Biological Engineering, Yichun University, Jiangxi 336000, China. ycxxxj@163.com.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Le
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. lwqp@163.com.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Lu
authors.affiliation: College of Chemical and Biological Engineering, Yichun University, Jiangxi 336000, China. ycxxxj@163.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. wuzhijun999@sina.com.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. chenwansheng@smmu.edu.cn.
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031176:Bidens / Q000737:chemistry*; D002460:Cell Line; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D053279:Polyynes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000093242:Interleukin-6 Inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D005960:Glucosides; D016753:Interleukin-10; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008156:Luciferases; D016328:NF-kappa B; D010666:Phenylpropionates; D010936:Plant Extracts; D053279:Polyynes; D014409:Tumor Necrosis Factor-alpha; D000093242:Interleukin-6 Inhibitors
keywords: Bidens frondosa; anti-inflammatory activity; flavonoid glycoside; phenylpropanoid glucoside; polyacetylene glucoside