title: Mycobacterium tuberculosis strains modify granular enzyme secretion and apoptosis of human neutrophils.
abstract: Mycobacterium tuberculosis has evolved to employ multiple strategies to avoid an efficient host immune response. Accordingly, enzymes are important antimycobacterial elements and apoptosis decides the fate of any cell. Hence, we carried out this study to discern the amplitude of two clinical stains (S7 & S10) and a laboratory strain (H37Rv) of M. tuberculosis in modifying the release of lytic enzymes and apoptosis of neutrophils from healthy volunteers and pulmonary tuberculosis patients. We detected reduced levels of elastase in neutrophils from pulmonary tuberculosis patients. The laboratory strain H37Rv is found to increase the release of elastase and myeloperoxidase in neutrophils from both the groups. This strain is more efficient compared to clinical strains in inducing late apoptosis/necrosis in neutrophils. Our results proclaim the susceptibility of neutrophils in responding to infection with H37Rv. Also, at the functional level, neutrophils undergo changes related to release of enzymes after acquiring tuberculosis.
authors.affiliation: Department of Immunology, National Institute for Research in Tuberculosis, Chetput, Chennai 600031, India.
authors.forename: J Nancy
authors.initials: JN
authors.lastname: Hilda
authors.affiliation: Institute for Thoracic Medicine, Chetput, Chennai 600031, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Narasimhan
authors.affiliation: Department of Immunology, National Institute for Research in Tuberculosis, Chetput, Chennai 600031, India. Electronic address: sulochanadas@gmail.com.
authors.forename: Sulochana D
authors.initials: SD
authors.lastname: Das
mesh_terms: D000328:Adult; D017209:Apoptosis; D016022:Case-Control Studies; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D019272:Leukocyte Elastase / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D061251:Primary Cell Culture; D013482:Superoxide Dismutase / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014397:Tuberculosis, Pulmonary / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology / Q000473:pathology
chemical_list: D013482:Superoxide Dismutase; D019272:Leukocyte Elastase; D013755:Tetradecanoylphorbol Acetate
keywords: Apoptosis; Elastase; H37Rv; Myeloperoxidase; Neutrophil