title: Antioxidant comparative effects of two grape pomace Mexican extracts from vineyards on erythrocytes.
abstract: Maceration and Soxhlet methods were used to obtain methanol extracts from a Mexican grape (Ruby Cabernet) pomace and the biological activity and phenolic profiles were compared. The antioxidant capacity was used to evaluate the mechanism of action, using a physiological model (erythrocytes) of damage induced by AAPH-generated free radicals. The extract obtained by maceration presented a total phenolic content twice the one obtained using the Soxhlet method. It also contained the most potent antioxidants, reducing anisotropy in the presence of AAPH to the levels of untreated cells, restoring membrane fluidity, preventing the morphological changes, as demonstrated by scanning electron microscopy (SEM), and providing protection against protein oxidation at the higher concentration. Our work showed that both extracts presented significant antioxidant activity through positive interactions with the lipid bilayer.
authors.affiliation: Universidad Autónoma de Nuevo León (UANL), Facultad de Ciencias Biológicas, Departamento de Química, Laboratorio de Química Analítica, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico; Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXII s/n, 08028 Barcelona, Spain. Electronic address: ledygarcia@ub.edu.
authors.forename: Ledy
authors.initials: L
authors.lastname: García-Becerra
authors.affiliation: Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXII s/n, 08028 Barcelona, Spain. Electronic address: montsemitjans@ub.edu.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Mitjans
authors.affiliation: Universidad Autónoma de Nuevo León (UANL), Facultad de Ciencias Biológicas, Departamento de Química, Laboratorio de Química Analítica, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico. Electronic address: catalinarivas@yahoo.com.mx.
authors.forename: Catalina
authors.initials: C
authors.lastname: Rivas-Morales
authors.affiliation: Universidad Autónoma de Nuevo León (UANL), Facultad de Ciencias Biológicas, Departamento de Química, Laboratorio de Química Analítica, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico. Electronic address: lajulis_2000@yahoo.com.mx.
authors.forename: Julia
authors.initials: J
authors.lastname: Verde-Star
authors.affiliation: Universidad Autónoma de Nuevo León (UANL), Facultad de Ciencias Biológicas, Departamento de Química, Laboratorio de Química Analítica, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León C.P. 66451, Mexico. Electronic address: azucenaoranday@hotmail.com.
authors.forename: Azucena
authors.initials: A
authors.lastname: Oranday-Cárdenas
authors.affiliation: Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXII s/n, 08028 Barcelona, Spain. Electronic address: mpvinardellmh@ub.edu.
authors.forename: Pilar
authors.initials: P
authors.lastname: Vinardell María
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D004912:Erythrocytes / Q000187:drug effects*; D005609:Free Radicals; D008800:Mexico; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D010636:Phenols; D010936:Plant Extracts
keywords: AAPH; Membrane alterations; Mexican grape pomace