title: Improvement in the amine glass platform by bubbling method for a DNA microarray.
abstract: A glass platform with high sensitivity for sexually transmitted diseases microarray is described here. An amino-silane-based self-assembled monolayer was coated on the surface of a glass platform using a novel bubbling method. The optimized surface of the glass platform had highly uniform surface modifications using this method, as well as improved hybridization properties with capture probes in the DNA microarray. On the basis of these results, the improved glass platform serves as a highly reliable and optimal material for the DNA microarray. Moreover, in this study, we demonstrated that our glass platform, manufactured by utilizing the bubbling method, had higher uniformity, shorter processing time, lower background signal, and higher spot signal than the platforms manufactured by the general dipping method. The DNA microarray manufactured with a glass platform prepared using bubbling method can be used as a clinical diagnostic tool.
authors.affiliation: Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Jee
authors.affiliation: Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Ji Hyeong
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Yoon
mesh_terms: D000588:Amines / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D012821:Silanes / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
chemical_list: D000588:Amines; D012821:Silanes
keywords: DNA microarray; bubbling method; glass platform; self-assambled monolayer