title: Filterability of freshly-collected sickle erythrocytes under venous oxygen pressure without exposure to air.
abstract: We previously found that blood samples collected from steady-state patients with sickle cell disease (SCD) without exposure to air contain a new type of reversibly sickled cells (RSCs) with blunt edges at a level of as high as 78%. Since partial oxygenation of once-deoxygenated sickled cells with pointy edges to near venous oxygen pressure generates similar sickled cells with blunt edges in vitro, we named them as partially oxygenated sickled cells (POSCs). On the other hand, partial deoxygenation of once-oxygenated SS cells to venous oxygen pressure generates partially deoxygenated sickled cells (PDSCs) with pointy edges. In this study, we obtained blood samples from 6 steady-state patients with SCD under venous oxygen pressure without exposure to air, subjected them to various oxygenation/deoxygenation/reoxygenation cycles, and studied their filterability through a membrane filter with pore diameter of 3μm, the theoretical minimum diameter of a capillary. Our results indicated that discocytes, POSCs with blunt edges, and irreversibly sickled cells could deform and pass through the filter, while PDSCs with pointy edges were rigid and could not. The filterability of SS cells seems to be related to the length and amount of deoxy-hemoglobin S fibers in the cells.
authors.affiliation: Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
authors.forename: Siddharth
authors.initials: S
authors.lastname: Shah
authors.affiliation: Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
authors.forename: Rhonda Graves
authors.initials: RG
authors.lastname: Acholonu
authors.affiliation: Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.
authors.forename: Kwaku
authors.initials: K
authors.lastname: Ohene-Frempong
authors.affiliation: Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA. Electronic address: siddharth.shah@uphs.upenn.edu.
authors.forename: Toshio
authors.initials: T
authors.lastname: Asakura
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood* / Q000378:metabolism*; D001784:Blood Gas Analysis; D004913:Erythrocytes, Abnormal / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D010100:Oxygen / Q000378:metabolism*
chemical_list: D010100:Oxygen
keywords: Filterability; Partially deoxygenated sickled cells; Partially oxygenated sickled cells; Sickle cell disease