title: Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene.
abstract: Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qi-chao
authors.initials: QC
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming-hong
authors.initials: MH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xian-wen
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Chao-yang
authors.initials: CY
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhi-cheng
authors.initials: ZC
authors.lastname: Shen
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004361:Drug Tolerance; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods; D005998:Glycine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism*; D007313:Insecta / Q000502:physiology*; D007306:Insecticides / Q000378:metabolism; D012275:Oryza / Q000187:drug effects / Q000469:parasitology / Q000502:physiology*; D010572:Pest Control, Biological / Q000379:methods; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000469:parasitology / Q000502:physiology; D014162:Transfection / Q000379:methods*; D000097797:Glyphosate
chemical_list: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004731:Endotoxins; D005998:Glycine; D006460:Hemolysin Proteins; D007306:Insecticides; D000097797:Glyphosate; C036483:T-DNA; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bt gene stacking; Bt resistance management; EPSPS; Transgenic rice