title: Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?
abstract: In this study Ocimum basilicum L. (OB) and Ocimum tenuiflorum L. (OT) in vitro culture standardisation for increasing eugenol distribution, in comparison to their respective field grown parts was carried out. Eugenol was quantified using an optimised HPLC method and its relation with the total phenolic content (TPC) was measured. In vitro grown leaves and somatic embryos, of both OB and OT were found to contain similar quantities of eugenol (85μg/g approximately), higher than OB and OT field-grown leaves (30.2μg/g and 25.1μg/g respectively). It was also determined that in vitro grown leaves were richer in TPC than the field-grown intact organs. Results demonstrated the prominence of in vitro cultures for eugenol extraction. This study underlines that important food flavouring metabolites (e.g. vanillin, vanillic acids) might be produced, via the eugenol pathway, in Ocimum species that may be a good potential source of eugenol.
authors.affiliation: Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India.
authors.forename: Karuppiah
authors.initials: K
authors.lastname: Bhuvaneshwari
authors.affiliation: Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India; Korean Ginseng Center and Ginseng Resource Bank, Kyung Hee University, Yongin 449-701, Republic of Korea.
authors.forename: Ananda
authors.initials: A
authors.lastname: Gokulanathan
authors.affiliation: Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India.
authors.forename: Malayandi
authors.initials: M
authors.lastname: Jayanthi
authors.affiliation: Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India.
authors.forename: Vaithiyanathan
authors.initials: V
authors.lastname: Govindasamy
authors.affiliation: Department of Science, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Milella
authors.affiliation: Department of Computer Engineering, Kyung Hee University, Yongin 449-701, Republic of Korea.
authors.forename: Sungyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Korean Ginseng Center and Ginseng Resource Bank, Kyung Hee University, Yongin 449-701, Republic of Korea. Electronic address: dcyang@khu.ac.kr.
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
authors.affiliation: Plant Metabolic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamilnadu 641 046, India. Electronic address: girijabiotech@yahoo.co.in.
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Girija
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005054:Eugenol / Q000737:chemistry*; D066298:In Vitro Techniques; D018649:Ocimum basilicum / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D005054:Eugenol
keywords: Eugenol; In vitro; Ocimum basilicum; Ocimum tenuiflorum; RP-HPLC; Total phenolic content