title: Label-free electrochemical immunosensor based on cerium oxide nanowires for Vibrio cholerae O1 detection.
abstract: This paper developed a label-free immunosensor based on cerium oxide nanowire for Vibrio cholerae O1 detection application. The CeO2 nanowires were synthesized by hydrothermal reaction. The immobilization of Anti-V. cholerae O1 onto CeO2 nanowire-deposited sensor was performed via an amino ester, which was created by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and sulfo-N-hydroxysuccinimide. The electrochemical responses of the immunosensor were studied by electrochemical impedance spectroscopy with [Fe (CN) 6] (3-/4-) as redox probe. A linear response in electron transfer resistance for cell of V. cholerae O1 concentration was found in the range of 1.0 × 10(2)CFU/mL to 1.0 × 10(4)CFU/mL. The detection limit of the immunosensor was 1.0 × 10(2)CFU/mL. The immunosensor sensitivity was 56.82 Ω/CFU · mL(-1). Furthermore, the parameters affecting immunosensor response were also investigated, as follows: pH value, immunoreaction time, incubation temperature, and anti-V. cholerae O1 concentration.
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam. Electronic address: phuongdinhtam@gmail.com.
authors.forename: Phuong Dinh
authors.initials: PD
authors.lastname: Tam
authors.affiliation: Advanced Institute for Science and Technology (AIST), Hanoi University of Science and Technology, Viet Nam. Electronic address: thang.caoxuan@hust.edu.vn.
authors.forename: Cao Xuan
authors.initials: CX
authors.lastname: Thang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D002234:Carbodiimides / Q000737:chemistry; D002563:Cerium / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D053770:Nanowires / Q000737:chemistry*; D013388:Succinimides / Q000737:chemistry; D013696:Temperature; D041241:Vibrio cholerae O1 / Q000302:isolation & purification*
chemical_list: D055910:Antibodies, Immobilized; D002234:Carbodiimides; D013388:Succinimides; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D002563:Cerium; C030583:ceric oxide; C001426:N-hydroxysuccinimide
keywords: Antibody; Antigen; CeO(2); Immunosensor; Nanowire