title: Electrospun PDLLA/PLGA composite membranes for potential application in guided tissue regeneration.
abstract: With the aim to explore a membrane system with appropriate degradation rate and excellent cell-occlusiveness for guided tissue regeneration (GTR), a series of poly(D, L-lactic acid) (PDLLA)/poly(D, L-lactic-co-glycolic acid) (PLGA) (100/0, 70/30, 50/50, 30/70, 0/100, w/w) composite membranes were fabricated via electrospinning. The fabricated membranes were evaluated by morphological characterization, water contact angle measurement and tensile test. In vitro degradation was characterized in terms of the weight loss and the morphological change. Moreover, in vitro cytologic research revealed that PDLLA/PLGA composite membranes could efficiently inhibit the infiltration of 293 T cells. Finally, subcutaneous implant test on SD rat in vivo showed that PDLLA/PLGA (70/30, 50/50) composite membranes could function well as a physical barrier to prevent cellular infiltration within 13 weeks. These results suggested that electrospun PDLLA/PLGA (50/50) composite membranes could serve as a promising barrier membrane for guided tissue regeneration due to suitable biodegradability, preferable mechanical properties and excellent cellular shielding effects.
authors.affiliation: School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China.
authors.forename: Ershuai
authors.initials: E
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China.
authors.forename: Chuanshun
authors.initials: C
authors.lastname: Zhu
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Suhua
authors.initials: S
authors.lastname: Li
authors.affiliation: Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China. Electronic address: yaofanglian@tju.edu.cn.
authors.forename: Fanglian
authors.initials: F
authors.lastname: Yao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D048091:Guided Tissue Regeneration; D057809:HEK293 Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Electrospinning; Guided tissue regeneration; Microfiber membranes; Poly(d,l-lactic-co-glycolic acid) (PLGA); Poly(dl-lactic acid) (PDLLA)