title: Agglutination Assays of the Plasmodium falciparum-Infected Erythrocyte.
abstract: The agglutination assay is used to determine the ability of antibodies to recognize parasite variant antigens on the surface of Plasmodium falciparum-infected erythrocytes. In this technique, infected erythrocytes are selectively labelled with a DNA-binding fluorescent dye and mixed with antibodies of interest to allow antibody-surface antigen binding. Recognition of surface antigens by the antibodies can result in the formation of agglutinates containing multiple parasite-infected erythrocytes. These can be viewed and quantified using a fluorescence microscope.
authors.affiliation: KEMRI-Wellcome Trust Research Programme, 230-80108, Kilifi, Kenya.|Centre for Tropical Medicine, Nuffield Department of Medicine, Oxford University, Oxford, OX3 7LJ, UK.
authors.forename: Joshua
authors.initials: J
authors.lastname: Tan
authors.affiliation: KEMRI-Wellcome Trust Research Programme, 230-80108, Kilifi, Kenya. PBull@kemri-wellcome.org.|Centre for Tropical Medicine, Nuffield Department of Medicine, Oxford University, Oxford, OX3 7LJ, UK. PBull@kemri-wellcome.org.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Bull
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000097:blood / Q000302:isolation & purification; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000469:parasitology; D005434:Flow Cytometry; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface
keywords: Agglutination; Antibody; Infected erythrocytes; Malaria; Naturally acquired immunity; PfEMP1; Plasmodium falciparum; Variant surface antigens