title: [Protective effect of phloroglucinol on renal ischemia and reperfusion injury].
abstract: OBJECTIVE
  To investigate the effect and mechanisms of Phloroglucinol (PG) on renal ischemia and reperfusion injury (IRI).
  
  
  METHODS
  Forty-eight male Wistar rats were divided into 3 groups (16 rats per group): sham operated, saline-treated I/R (I/R), and PG-treated I/R (PG). I/R model: After removing the right kidney, renal I/R injury was induced by clamping the left renal artery for 45 min followed by reperfusion. The rats were administered with PG (30 mg/kg, intraperitoneally) or saline 15 min before renal ischemia. The blood and kidneys were harvested 6 and 24 h after reperfusion. Renal function and histologic changes of serum creatinine (SCr) and blood urea nitrogen(BUN)were assessed. Malondialdehyde (MDA),catalase (CAT),superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px)were measured. Nuclear factor-kapa B (NF-κB) and caspase-3 in the kidneys were also measured.
  
  
  RESULTS
  SCr and BUN were (103.9±10.4) μmol/L and (15.2±1.0) mmol/L in I/R group, and (81.8±13.4) μmol/L and (11.5±1.2) mmol/L in PG group 6 h after reperfusion. SCr and BUN were (154.9±12.1) μmol/L and (28.1±1.4) mmol/L in I/R group, (103.8±5.9) μmol/L and (16.0±1.0) mmol/L in PG group 24 h after reperfusion.PG treatment significantly attenuated renal dysfunction and histologic damage caused by I/R injury (P<0.05).The I/R-induced elevation in kidney MDA level decreased, where as reduced kidney SOD,CAT and GSH-Px were increased. What is more, the apoptotic tubular cells, the levels of active caspase-3,and active nuclear factor kappa B dramatically decreased after PG treatment.
  
  
  CONCLUSIONS
  PG protects murine kidney I/R injury by suppressing oxidative stress, inflammation, and cell apoptosis.
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Hong-xian
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yun-peng
authors.initials: YP
authors.lastname: Wang
authors.affiliation: Special Medical Department, General Navy Hospital, Beijing 100048, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Hong
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Tian
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Lu-lin
authors.initials: LL
authors.lastname: Ma
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy*; D000818:Animals; D017209:Apoptosis; D001806:Blood Urea Nitrogen; D053148:Caspase 3 / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000473:pathology*; D007677:Kidney Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D010696:Phloroglucinol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D008315:Malondialdehyde; D010696:Phloroglucinol; D002374:Catalase; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3