title: Down-regulation of microRNA152 is associated with the diagnosis and prognosis of patients with osteosarcoma.
abstract: Potential values of microRNA152 (miR-152) as a serum diagnostic and prognostic biomarker have not been determined in human osteosarcoma. By detecting the expression of miR-152 among 80 osteosarcoma patients, 20 periostitis patients and 20 healthy individuals using qRT-PCR, we aimed to explore the clinical significance of miR-152 in osteosarcoma patients. The expression of miR-152 was significantly decreased in patients with osteosarcoma compared to patients with periostitis (P<0.01) and healthy controls (P<0.01). The relationship between clinicopathologic characteristics and miR-152 was analyzed by chi-square test. The outcome indicated that miR-152 might be linked with the development of osteosarcoma. Moreover, the receiver operating characteristic (ROC) curve was performed to estimate the diagnostic value of miR-152. The result demonstrated that miR-152 might be a promising diagnostic marker of osteosarcoma with an AUC of 0.956, combing with 92.5% specificity and 96.2% sensitivity. The relationship between miR-152 and overall survival of osteosarcoma patients was analyzed by Kaplan-Meier curve and log rank test. As a result, the survival time of patients with low miR-152 expression was significantly shorter than those with high miR-152 expression (P<0.001). Then Cox regression analysis was used to estimate the prognostic value of miR-152 in osteosarcoma. The outcomes showed that low miR-152 expression (P=0.004) might be a potential independent prognostic marker for osteosarcoma patients. These findings suggested that down-regulation of miR-152 could be considered as a predictor for diagnosis and prognosis of osteosarcoma patients.
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Nai-Guo
authors.initials: NG
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Bing-Yi
authors.initials: BY
authors.lastname: Tan
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
authors.forename: Ze-Nong
authors.initials: ZN
authors.lastname: Yuan
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D011379:Prognosis; D015996:Survival Rate; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C550856:MIRN152 microRNA, human; D035683:MicroRNAs
keywords: diagnosis; miR-152; osteosarcoma; prognosis