title: Qualitative and Quantitative Analysis of the Major Constituents in Shexiang Tongxin Dropping Pill by HPLC-Q-TOF-MS/MS and UPLC-QqQ-MS/MS.
abstract: Shexiang Tongxin dropping pill (STP) is a traditional Chinese medicine formula that consists of total saponins of ginseng, synthetic Calculus bovis, bear gall, Venenum bufonis, borneol and Salvia miltiorrhiza. STP has been widely used in China and Southeast Asia for the treatment of cardiovascular diseases. In this study, a qualitative analytical method using high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry was developed for identification of the major constituents in STP. Based on the retention time and MS spectra, 41 components were identified by comparison with reference compounds and literature data. Moreover, using ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode, we quantified 13 of the identified constituents (ginsenoside Rg1, ginsenoside Rk3, cinobufagin, arenobufagin, bufalin, resibufogenin, tanshinone IIA, taurine, tauroursodeoxycholic acid, taurocholic acid, cholic acid, deoxycholic acid, and chenodeoxycholic acid). These results suggest that this new approach is applicable for the routine analysis and quality control of STP products and provides fundamental data for further in vivo pharmacokinetical studies.
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. cdx1125@126.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. cdx1125@126.com.
authors.forename: Daxin
authors.initials: D
authors.lastname: Chen
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. lisa3350@163.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. lisa3350@163.com.
authors.forename: Shan
authors.initials: S
authors.lastname: Lin
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. yaoxuexuwei@163.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. hmq1115@126.com.
authors.forename: Mingqing
authors.initials: M
authors.lastname: Huang
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. jianfengchu@126.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. jianfengchu@126.com.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Chu
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. ybxwdj@hotmail.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. ybxwdj@hotmail.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. jiumaolin@hotmail.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. jiumaolin@hotmail.com.
authors.forename: Jiumao
authors.initials: J
authors.lastname: Lin
authors.affiliation: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. pjunlab@hotmail.com.|Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China. pjunlab@hotmail.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
mesh_terms: D001647:Bile Acids and Salts / Q000737:chemistry / Q000302:isolation & purification*; D002018:Bufanolides / Q000737:chemistry / Q000302:isolation & purification*; D002316:Cardiotonic Agents / Q000737:chemistry*; D002318:Cardiovascular Diseases / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013654:Taurine / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D001647:Bile Acids and Salts; D002018:Bufanolides; D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; C000600920:shexiang tongxin; D013654:Taurine
keywords: Shexiang Tongxin dropping pill; liquid chromatography; quadrupole time-of-flight tandem mass spectrometry; triple-quadrupole tandem