title: A novel dendritic-cell-targeting DNA vaccine for hepatitis B induces T cell and humoral immune responses and potentiates the antivirus activity in HBV transgenic mice.
abstract: Strategies for inducing an effective immune response following vaccination have focused on targeting antigens to dendritic cells (DCs) through the DC-specific surface molecule DEC-205. The immunogenicity and efficacy of DNA vaccination can also be enhanced by fusing the encoded antigen to single-chain antibodies directed against DEC-205. Here, we investigated this promising approach for its enhancement of hepatitis B virus (HBV)-specific cellular and humoral immune responses and its antiviral effects in HBV transgenic mice. A plasmid DNA vaccine encoding mouse DEC-205 single-chain fragment variable (mDEC-205-scFv) linked with the hepatitis B surface antigen (HBsAg) was constructed. Vaccination with this fusion DNA vaccine in HBV transgenic mice induced robust antiviral T cell and antibody immunity against HBsAg. The levels of serum-circulating HBsAg and the HBV DNA copy number were downregulated by the induction of a higher HBsAg-specific response. Thus, in this study, we demonstrated the therapeutic efficacy of the novel mDEC-205-scFv-fused DNA vaccine in a mouse model of immune-tolerant, chronic HBV infection.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Debin
authors.initials: D
authors.lastname: Yu
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Shi
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Hongge
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Nuoting
authors.initials: N
authors.lastname: Wu
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Cai
authors.affiliation: Zhejiang Normal University-Jinhua People's Hospital Joint Center for Biomedical Research, Jinhua, China. Electronic address: zoulibo1969@163.com.
authors.forename: Libo
authors.initials: L
authors.lastname: Zou
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China. Electronic address: Jobzou@163.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zou
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D057809:HEK293 Cells; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000235:genetics / Q000276:immunology; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology*; D037181:Lectins, C-Type / Q000235:genetics / Q000276:immunology; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C093569:DEC-205 receptor; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D057127:Single-Chain Antibodies; D019444:Vaccines, DNA
keywords: DC-targeting; DNA vaccine; Hepatitis B; Vaccine