title: Development of minimal fermentation media supplementation for ethanol production using two Saccharomyces cerevisiae strains.
abstract: Ethanol production by fermentation is strongly dependent on media composition. Specific nutrients, such as trace elements, vitamins and nitrogen will affect the physiological state and, consequently, the fermentation performance of the micro-organism employed. The purpose of this study has been to assess the highest ethanol production by a minimal medium, instead of the more complex nutrients supplementation used during alcoholic fermentation. All fermentation tests were carried out using a microwell plate reader to monitor the processes. Two Saccharomyces cerevisiae strains (NCYC 2826 and NCYC 3445) were tested using three nitrogen sources, supplied with different vitamin and salts. The results show that solutions made of urea phosphate, KCl, MgSO4·7H2O, Ca-panthothenate, biotin allowed an ethanol yield of 22.9 and 23.4 g/L for strain NCYC 2826 and NCYC 3445, respectively, representing 90 and 92% of the theoretical yield. All tests were carried out using glucose as common reference carbon source.
authors.affiliation: a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Tropea
authors.affiliation: b Institute of Food Research , Norwich Research Park , Norwich , UK.
authors.forename: David
authors.initials: D
authors.lastname: Wilson
authors.affiliation: a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Cicero
authors.affiliation: a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
authors.forename: Angela G
authors.initials: AG
authors.lastname: Potortì
authors.affiliation: a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
authors.forename: Giovanna L
authors.initials: GL
authors.lastname: La Torre
authors.affiliation: a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Dugo
authors.affiliation: c School of Biological Sciences , University of East Anglia , Norwich , UK.
authors.forename: David
authors.initials: D
authors.lastname: Richardson
authors.affiliation: b Institute of Food Research , Norwich Research Park , Norwich , UK.
authors.forename: Keith W
authors.initials: KW
authors.lastname: Waldron
mesh_terms: D000645:Ammonium Sulfate / Q000378:metabolism; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012492:Salts / Q000032:analysis / Q000378:metabolism; D014508:Urea / Q000378:metabolism; D014815:Vitamins / Q000032:analysis / Q000378:metabolism
chemical_list: D003470:Culture Media; D012492:Salts; D014815:Vitamins; D000431:Ethanol; D014508:Urea; D005947:Glucose; D009584:Nitrogen; D000645:Ammonium Sulfate
keywords: Ethanol; Saccharomyces cerevisiae; alcohol production; fermentation media; urea phosphate; yeast