title: In vitro studies of serum albumin interaction with poly(D,L-lactide) nanospheres loaded by hydrophobic cargo.
abstract: The various polymer-based nanocarriers are very attractive for in vitro and in vivo bioapplications. A new type of a promising drug delivery systems for cancer tissues-poly(D,L-lactide) nanospheres stabilized with Cremophor EL and loaded with hydrophobic cyanines (IR-780 or ZnPc) or curcumin (CUR) were fabricated by the nanoprecipitation method. The Cremophor EL/PLA/water nanospheres demonstrated regular shape, low polydispersity (PdI<0.3) and high entrapment efficiency of selected cargo (over 90%). The size of those nanoconstructs below 130 nm are in the desired nanocarriers size range for tumor delivery. Low level of in vitro drug release from loaded nanospheres after long-time storage indicates their good stability. The half-life of nanocarriers in the circulation, and their biodistribution after parenteral administration are associated with the ability of plasma proteins adsorption. For these reasons the affinity of obtained nanospheres for albumin as a major plasma protein was in vitro investigated. The binding of nanocarrier containing cyanine IR-780 with albumin immobilized in the wells of polystyrene plate occurred with lower efficiency than analogs loaded with ZnPc or CUR. Similar relationships were observed after UV-vis spectra analysis of nanospheres in the presence of albumin at various protein concentrations.
authors.affiliation: Department of Medical Biochemistry, Medical University of Wroclaw, Chalubinskiego 10, 50-368 Wroclaw, Poland. Electronic address: jadwiga.pietkiewicz@umed.wroc.pl.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Pietkiewicz
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Kazimiera A
authors.initials: KA
authors.lastname: Wilk
authors.affiliation: Department of Organic and Pharmaceutical Technology, Faculty of Chemistry, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Bazylińska
mesh_terms: D000818:Animals; D002417:Cattle; D003474:Curcumin / Q000008:administration & dosage / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D007208:Indocyanine Green / Q000008:administration & dosage / Q000378:metabolism; D054118:Nanospheres / Q000008:administration & dosage / Q000378:metabolism*; D010316:Particle Size; D011091:Polyesters / Q000008:administration & dosage / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D012710:Serum Albumin, Bovine; C033616:poly(lactide); D003474:Curcumin; D007208:Indocyanine Green
keywords: Colloidal stability; Nanosphere-serum albumin complexes; Polymeric nanocarriers