title: Integrated method of thermosensitive triblock copolymer-salt aqueous two phase extraction and dialysis membrane separation for purification of lycium barbarum polysaccharide.
abstract: A polymer-salt aqueous two-phase system (ATPS) consisting of thermosensitive copolymer ethylene-oxide-b-propylene-oxide-b-ethylene-oxide (EOPOEO) and NaH2PO4 was employed in deproteinization for lycium barbarum polysaccharide (LBP). The effects of salt type and concentration, EOPOEO concentration, amount of crude LBP solution and temperature were studied. In the primary extraction process, LBP was preferentially partitioned to the bottom (salt-rich) phase with high recovery ratio of 96.3%, while 94.4% of impurity protein was removed to the top (EOPOEO-rich) phase. Moreover, the majority of pigments could be discarded to top phase. After phase-separation, the LBP in the bottom phase was further purified by dialysis membrane to remove salt and other small molecular impurities. The purity of LBP was enhanced to 64%. Additionally, the FT-IR spectrum was used to identify LBP. EOPOEO was recovered by a temperature-induced separation, and reused in a new ATPS. An ideal extraction and recycle result were achieved.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China. Electronic address: yunwang@ujs.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Hu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Ni
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Tang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Zhenjiang Entry-exit Inspection Quarantine Bureau, State Key Laboratory of Food Additive and Condiment Testing, 84 Dongwu Road, Zhenjiang 212013, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D032305:Lycium / Q000737:chemistry*; D008567:Membranes, Artificial; D010710:Phosphates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D011506:Proteins / Q000302:isolation & purification*; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D004365:Drugs, Chinese Herbal; D008567:Membranes, Artificial; C116176:PEO-PPO-PEO; D010710:Phosphates; D011409:Propylene Glycols; D011506:Proteins; D012996:Solutions; C500223:lycium barbarum polysaccharide; D011092:Polyethylene Glycols; C018279:sodium phosphate
keywords: Aqueous two phase system; Lycium barbarum polysaccharide; Purification; Recycling; Thermosensitive polymer