title: Belliella buryatensis sp. nov., isolated from alkaline lake water.
abstract: Two bacterial isolates from water of the alkaline brackish Lake Solenoe (Buryatia, Russia), 2C and 5CT, were characterized by using a polyphasic taxonomic approach. The strains were small, non-motile, Gram-stain-negative rods that formed small orange-red colonies on the surface of marine agar. Studies based on 16S rRNA gene sequences showed that the strains were related closely to Belliella pelovolcani CC-SAL-25T (98.7 % sequence similarity). The G+C content of the DNA was 38-40 mol%. DNA-DNA hybridization values between strains 2C and 5CT and B. pelovolcani CC-SAL-25T were 56-58 mol%. A menaquinone with seven isoprene units (MK-7) was the major respiratory quinone. The fatty acid profiles were slightly different from that of B. pelovolcani CC-SAL-25T. The novel strains could be distinguished from the phylogenetically closest species B. pelovolcani CC-SAL-25T based on matrix-assisted laser desorption ionization time-of-flight mass spectra of whole cells and a range of physiological and biochemical characteristics. The data obtained suggest that strains 2C and 5CT represent a novel species of the genus Belliella, for which the name Belliella buryatensis sp. nov. is proposed. The type strain is 5CT ( = VKM B-2724T = KCTC 32194T).
authors.affiliation: Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 6, Sakhyanovoy str., Ulan-Ude, 670047, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Kozyreva
authors.affiliation: Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 6, Sakhyanovoy str., Ulan-Ude, 670047, Russia.
authors.forename: Darya
authors.initials: D
authors.lastname: Egorova
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 13, Goleva str., Perm, 614081, Russia.
authors.forename: Lyudmila
authors.initials: L
authors.lastname: Anan'ina
authors.affiliation: Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 13, Goleva str., Perm, 614081, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Plotnikova
authors.affiliation: All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Ariskina
authors.affiliation: All-Russian Collection of Microorganisms (VKM), G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Prisyazhnaya
authors.affiliation: Baikal Institute of Nature Management, Siberian Branch, Russian Academy of Sciences, 6, Sakhyanovoy str., Ulan-Ude, 670047, Russia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Radnaeva
authors.affiliation: Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, 6, Sakhyanovoy str., Ulan-Ude, 670047, Russia.
authors.forename: Bair
authors.initials: B
authors.lastname: Namsaraev
mesh_terms: D000468:Alkalies; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000468:Alkalies; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7