title: A SERS protocol as a potential tool to access 6-mercaptopurine release accelerated by glutathione-S-transferase.
abstract: The developed method for monitoring GST, an important drug metabolic enzyme, could greatly facilitate researches on relative biological fields. In this work, we have developed a SERS technique to monitor the absorbance behaviour of 6-mercaptopurine (6-MP) and its glutathione-S-transferase (GST)-accelerated glutathione (GSH)-triggered release behaviour on the surface of gold nanoflowers (GNFs), using the GNFs as excellent SERS substrates. The SERS signal was used as an indicator of absorbance or release of 6-MP on the gold surface. We found that GST can accelerate GSH-triggered release behaviour of 6-MP from the gold surface. We speculated that GST catalyzes nucleophilic GSH to competitively bind with the electrophilic substance 6-MP. Experimental results have proved that the presented SERS protocol can be utilized as an effective tool for accessing the release of anticancer drugs.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Qingran
authors.initials: Q
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. wqian@seu.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Qian
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D003692:Delayed-Action Preparations / Q000737:chemistry*; D065546:Drug Liberation; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D015122:Mercaptopurine / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D000964:Antimetabolites, Antineoplastic; D003692:Delayed-Action Preparations; D006046:Gold; D015122:Mercaptopurine; D005982:Glutathione Transferase; D005978:Glutathione