title: In Vitro Models for the Study of the Intracellular Activity of Antibiotics.
abstract: Intracellular bacteria are poorly responsive to antibiotic treatment. Pharmacological studies are thus needed to determine which antibiotics are most potent or effective against intracellular bacteria as well as to explore the reasons for poor bacterial responsiveness. An in vitro pharmacodynamic model is described, consisting of (1) phagocytosis of pre-opsonized bacteria by eukaryotic cells; (2) elimination of non-internalized bacteria with gentamicin; (3) incubation of infected cells with antibiotics; and (4) determination of surviving bacteria by viable cell counting and normalization of the counts based on sample protein content.
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.|Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Julien M
authors.initials: JM
authors.lastname: Buyck
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.|GSK Biologicals, Rixensart, Belgium.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lemaire
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.|Department of Microbiology, Hospital Clínico Universitario Lozano Blesa, Zaragoza, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Seral
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Ahalieyah
authors.initials: A
authors.lastname: Anantharajah
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Peyrusson
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Tulkens
authors.affiliation: Pharmacologie cellulaire et moléculaire, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium. francoise.vanbambeke@uclouvain.be.|Pharmacologie cellulaire et molÕculaire, Louvain Drug Research Institute, Avenue E. Mounier 73 B1.73.05, Brussels, 1200, Belgium. francoise.vanbambeke@uclouvain.be.
authors.forename: Françoise
authors.initials: F
authors.lastname: Van Bambeke
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects / Q000382:microbiology; D005839:Gentamicins / Q000494:pharmacology*; D009000:Monocytes / Q000382:microbiology; D010587:Phagocytosis / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D005839:Gentamicins
keywords: Antibiotic; Efficacy; Gentamicin; Intracellular infection; Opsonization; Phagocytosis; Pharmacodynamics; Relative potency