title: Apoptotic effects of salinomycin on human ovarian cancer cell line (OVCAR-3).
abstract: In this study, we studied the apoptotic and cytotoxic effects of salinomycin on human ovarian cancer cell line (OVCAR-3) as salinomycin is known as a selectively cancer stem cell killer agent. We used immortal human ovarian epithelial cell line (IHOEC) as control group. Ovarian cancer cells and ovarian epithelial cells were treated by different concentrations of salinomycin such as 0.1, 1, and 40 μM and incubated for 24, 48, and 72 h. Dimethylthiazol (MTT) cell viability assay was performed to determine cell viability and toxicity. On the other hand, the expression levels of some of the apoptosis-related genes, namely anti-apoptotic Bcl-2, apoptotic Bax, and Caspase-3 were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, Caspase-3 protein level was also determined. As a result, we concluded that incubation of human OVCAR-3 by 0.1 μM concentration of salinomycin for 24 h killed 40 % of the cancer cells by activating apoptosis but had no effect on normal cells. The apoptotic Bax gene expression was upregulated but anti-apoptotic Bcl-2 gene expression was downregulated. Active Caspase-3 protein level was increased significantly (p < 0.05).
authors.affiliation: Health Science Institute, Biochemistry, Hacettepe University, Ankara, Turkey. fuat_kaplan@hotmail.com.
authors.forename: Fuat
authors.initials: F
authors.lastname: Kaplan
authors.affiliation: Medical Biology Department, Faculty of Medicine, Ankara University, Ankara, Turkey. fteksen58@windowslive.com.
authors.forename: Fulya
authors.initials: F
authors.lastname: Teksen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011714:Pyrans / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D000095662:Polyether Polyketides
chemical_list: D000900:Anti-Bacterial Agents; D051017:Apoptosis Regulatory Proteins; D053148:Caspase 3; D019253:Proto-Oncogene Proteins c-bcl-2; D011714:Pyrans; D051028:bcl-2-Associated X Protein; C010327:salinomycin; D000095662:Polyether Polyketides
keywords: Apoptosis; Cell viability; Ovarian cancer; Salinomycin