title: Preparation of Polyion Complex Micelles Using Block Copolymers for SiRNA Delivery.
abstract: Polyion complex (PIC) micelles can be prepared through the spontaneous assembly of cationic block copolymers with oppositely charged short interfering RNAs (SiRNAs). Their core-shell architectures offer a delivery platform for vulnerable SiRNA, improving their biological activities for medicinal applications such as tumor-targeted therapy. Here, we report a protocol for the preparation of SiRNA-loaded PIC micelles using a poly(ethylene glycol)-block-poly(aspartamide) derivative, providing the physicochemical criteria for well-defined micellar formulation. In addition, we describe protocols for a stability assay for SiRNA-loaded PIC micelles in the presence of serum using fluorescence correlation spectroscopy and a luciferase assay for cultured cancer cells stably expressing luciferase, thus providing the biological criteria for further medicinal applications.
authors.affiliation: Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.|Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
authors.forename: Meng
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Kanjiro
authors.initials: K
authors.lastname: Miyata
authors.affiliation: Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. kataoka@bmw.t.u-tokyo.ac.jp.|Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. kataoka@bmw.t.u-tokyo.ac.jp.|Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan. kataoka@bmw.t.u-tokyo.ac.jp.|Kawasaki Institute of Industry Promotion, 66-20 Horikawa-cho, Saiwai-ku, Kawasaki, 212-0013, Japan. kataoka@bmw.t.u-tokyo.ac.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kataoka
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D006367:HeLa Cells; D006801:Humans; D008823:Micelles; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics; D014162:Transfection
chemical_list: D004337:Drug Carriers; D008823:Micelles; D011108:Polymers; D034741:RNA, Small Interfering
keywords: Block copolymer; Fluorescence correlation spectroscopy; Polyion complex micelle; RNAi; SiRNA delivery