title: The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay.
abstract: The quantification of single cell interferon-gamma (IFN-γ) release for assessing cellular immune responses using the Enzyme-linked immunospot (ELISPOT) assay is an invaluable technique in immunology. Peripheral blood mononuclear cells (PBMC) are stimulated in vitro with recombinant proteins, peptides and recently whole malaria organisms. Stimulation may be short term (20-36 h) or long term (cultured ELISpot, up to 7 days). ELISpot is also able to quantify other cytokines secreted by antigen-specific T-cells, such as interleukin-2, interleukin-5, and other interleukins. ELISpot is playing an important role especially in vaccine research studies.
authors.affiliation: Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA. martha.sedegah@med.navy.mil.
authors.forename: Martha
authors.initials: M
authors.lastname: Sedegah
mesh_terms: D000953:Antigens, Protozoan / Q000276:immunology; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D006801:Humans; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism*; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D015848:Interleukin-5 / Q000276:immunology / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000469:parasitology; D059010:Single-Cell Analysis; D013601:T-Lymphocytes / Q000276:immunology / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan; C508599:IL5 protein, human; D007376:Interleukin-2; D015848:Interleukin-5; D007371:Interferon-gamma
keywords: Cytokines; ELISPOT; IFN-γ; Interferon-gamma; PBMC; Peripheral blood mononuclear cells; T cells