title: Ultrasensitive detection of amifostine and alkaline phosphatase based on the growth of CdS quantum dots.
abstract: In this study, we reported a simple and sensitive fluorescence nanosensor for rapid detection of amifostine and alkaline phosphatase (ALP). The novel nanosensor was based on the fluorescence "turn on-off" of CdS quantum dots (QDs). Firstly, Cd(2+) cation could react with S(2-) anion to generate fluorescent CdS QDs in the presence of amifostine. The fluorescence (FL) intensity of amifostine-capped CdS QDs (Amifostine-CdS QDs) was increased with the increasing amounts of amifostine, and could be used for amifostine detection. However, amifostine could be converted to 2-(3-aminopropylamino) ethanethiol (WR1065) in the presence of ALP based on the dephosphorylation of ALP. Under the optimum conditions, the affinity of WR1065 to CdS QDs was weaker than that of amifostine. Therefore the new generation of WR1065-CdS QDs would reduce the FL intensity with the increase of ALP concentration, and the fluorescence of CdS QDs was turn off. The metabolic process of amifostine in the presence of alkaline phosphatase could be also studied via the change of FL intensity of CdS QDs. The present method was cost-effective, convenient, and does not require any complicated synthetic procedures.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Weidan
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D004999:Amifostine / Q000032:analysis* / Q000737:chemistry; D019187:Cadmium Compounds / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D057230:Limit of Detection; D008624:Mercaptoethylamines / Q000737:chemistry; D036103:Nanotechnology / Q000295:instrumentation*; D010756:Phosphoric Acids / Q000737:chemistry; D010766:Phosphorylation; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013440:Sulfides / Q000737:chemistry*; D013997:Time Factors
chemical_list: D019187:Cadmium Compounds; D008624:Mercaptoethylamines; D010756:Phosphoric Acids; D013440:Sulfides; C034939:cadmium sulfide; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; C030242:phosphoric acid; D000469:Alkaline Phosphatase; D004999:Amifostine
keywords: Alkaline phosphatase; Amifostine; CdS quantum dots; Fluorescence detection