title: Optimizing the spectrofluorimetric determination of cefdinir through a Taguchi experimental design approach.
abstract: The aim of this work is to optimize a spectrofluorimetric method for the determination of cefdinir (CFN) using the Taguchi method. The proposed method is based on the oxidative coupling reaction of CFN and cerium(IV) sulfate. The quenching effect of CFN on the fluorescence of the produced cerous ions is measured at an emission wavelength (λ(em)) of 358 nm after excitation (λ(ex)) at 301 nm. The Taguchi orthogonal array L9 (3(4)) was designed to determine the optimum reaction conditions. The results were analyzed using the signal-to-noise (S/N) ratio and analysis of variance (ANOVA). The optimal experimental conditions obtained from this study were 1 mL of 0.2% MBTH, 0.4 mL of 0.25% Ce(IV), a reaction time of 10 min and methanol as the diluting solvent. The calibration plot displayed a good linear relationship over a range of 0.5-10.0 µg/mL. The proposed method was successfully applied to the determination of CFN in bulk powder and pharmaceutical dosage forms. The results are in good agreement with those obtained using the comparison method. Finally, the Taguchi method provided a systematic and efficient methodology for this optimization, with considerably less effort than would be required for other optimizations techniques.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura City, Egypt.
authors.forename: Noura Hemdan
authors.initials: NH
authors.lastname: Abou-Taleb
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura City, Egypt.
authors.forename: Dalia Rashad
authors.initials: DR
authors.lastname: El-Wasseef
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura City, Egypt.
authors.forename: Dina Tawfik
authors.initials: DT
authors.lastname: El-Sherbiny
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura City, Egypt.
authors.forename: Saadia Mohamed
authors.initials: SM
authors.lastname: El-Ashry
mesh_terms: D000077525:Cefdinir; D002511:Cephalosporins / Q000032:analysis*; D002563:Cerium / Q000737:chemistry*; D005453:Fluorescence; D015394:Molecular Structure; D012107:Research Design; D013050:Spectrometry, Fluorescence; D013431:Sulfates / Q000737:chemistry*
chemical_list: D002511:Cephalosporins; D013431:Sulfates; C107379:cerium sulfate; D002563:Cerium; D000077525:Cefdinir
keywords: MBTH; Taguchi orthogonal array; cefdinir; signal-to-noise ratio; spectrofluorimetry