title: Protective effect of rosmarinic acid against oxidative stress biomarkers in liver and kidney of strepotozotocin-induced diabetic rats.
abstract: In the present study, we investigated the efficiency of rosmarinic acid (RA) in preventing the alteration of oxidative parameters in the liver and kidney of diabetic rats induced by streptozotocin (STZ). The animals were divided into six groups (n = 8): control, ethanol, RA 10 mg/kg, diabetic, diabetic/ethanol, and diabetic/RA 10 mg/kg. After 3 weeks of treatment, we found that TBARS levels in liver and kidney were significantly increased in the diabetic/saline group and the administration of RA prevented this increase in the liver and kidney (P < 0.05). Diabetes caused a significant decrease in the activity of superoxide dismutase (SOD) and catalase (CAT) in the diabetes/saline group (P < 0.05). However, the treatment with 10 mg/kg RA (antioxidant) prevented this alteration in SOD and CAT activity in the diabetic RA group (P < 0.05). In addition, RA reverses the decrease in ascorbic acid and non-protein-thiol (NPSH) levels in diabetic rats. The treatment with RA also prevented the decrease in the Delta-aminolevulinic acid dehydratase (ALA-D) activity in the liver and kidney of diabetic rats. Furthermore, RA did not have any effect on glycemic levels. These results indicate that RA effectively reduced the oxidative stress induced by STZ, suggesting that RA is a potential candidate for the prevention and treatment of pathological conditions in diabetic models.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mushtaq
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil. roberta.schmatz@ibiruba.ifrs.edu.br.|Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, IFRS-Câmpus Ibirubá, 98200-000, Ibirubá, Brazil. roberta.schmatz@ibiruba.ifrs.edu.br.
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.affiliation: Department of Biotechnology, University of Science and Technology, Bannu, Khyber Pakhtunkhwa, Pakistan.
authors.forename: Mushtaq
authors.initials: M
authors.lastname: Ahmed
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Luciane Belmonte
authors.initials: LB
authors.lastname: Pereira
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Pauline
authors.initials: P
authors.lastname: da Costa
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Karine Paula
authors.initials: KP
authors.lastname: Reichert
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Diéssica
authors.initials: D
authors.lastname: Dalenogare
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Luana Paula
authors.initials: LP
authors.lastname: Pelinson
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Juliano Marchi
authors.initials: JM
authors.lastname: Vieira
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Lizielle Souza
authors.initials: LS
authors.lastname: de Oliveira
authors.affiliation: Department of NEUROFARBA, University of Florence, Via Ugo Schiff 6, Sesto F.no (Firenze), 50019, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mulinacci
authors.affiliation: Department of NEUROFARBA, University of Florence, Via Ugo Schiff 6, Sesto F.no (Firenze), 50019, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Bellumori
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil.
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900, Santa Maria, RS, Brazil. mariachitolina@gmail.com.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Schetinger
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose; D002934:Cinnamates / Q000494:pharmacology* / Q000627:therapeutic use; D053630:Depsides / Q000494:pharmacology* / Q000627:therapeutic use; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017208:Rats, Wistar; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid; D015415:Biomarkers; D001786:Blood Glucose; D002934:Cinnamates; D053630:Depsides; D008315:Malondialdehyde; D013311:Streptozocin; D013482:Superoxide Dismutase; D017392:Thiobarbituric Acid Reactive Substances; D000097123:Rosmarinic Acid
keywords: Oxidative stress; Rosmarinic acid; Strepotozotocin