title: [Study on Microbial Diversity of Peri-implantitis Subgingival by High-throughput Sequencing].
abstract: OBJECTIVE
  To study microbial diversity of peri-implantitis subgingival with high-throughput sequencing, and investigate microbiological etiology of peri-implantitis.
  
  
  METHODS
  Subgingival plaques were sampled from the patients with peri-implantitis (D group) and non-peri-implantitis subjects (N group). The microbiological diversity of the subgingival plaques was detected by sequencing V4 region of 16S rRNA with Illumina Miseq platform. The diversity of the community structure was analyzed using Mothur software.
  
  
  RESULTS
  A total of 156 507 gene sequences were detected in nine samples and 4 402 operational taxonomic units (OTUs) were found. Selenomonas, Pseudomonas, and Fusobacterium were dominant bacteria in D group, while Fusobacterium, Veillonella and Streptococcus were dominant bacteria in N group. Differences between peri-implantitis and non-peri-implantitis bacterial communities were observed at all phylogenetic levels by LEfSe, which was also found in PcoA test.
  
  
  CONCLUSIONS
  The occurrence of peri-implantitis is not only related to periodontitis pathogenic microbe, but also related with the changes of oral microbial community structure. Treponema, Herbaspirillum, Butyricimonas and Phaeobacte may be closely related to the occurrence and development of peri-implantitis.
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Li
authors.forename: Shao-guo
authors.initials: SG
authors.lastname: Wang
authors.forename: Yue-hong
authors.initials: YH
authors.lastname: Li
authors.forename: Dong-xiang
authors.initials: DX
authors.lastname: Tu
authors.forename: Shi-yun
authors.initials: SY
authors.lastname: Liu
authors.forename: Hong-bing
authors.initials: HB
authors.lastname: Nie
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Li
authors.forename: Ju-mei
authors.initials: JM
authors.lastname: Zhang
mesh_terms: D001419:Bacteria / Q000145:classification*; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D005673:Fusobacterium; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D057873:Peri-Implantitis / Q000382:microbiology*; D010518:Periodontitis; D010802:Phylogeny; D011549:Pseudomonas; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020357:Selenomonas; D017422:Sequence Analysis, DNA; D013291:Streptococcus; D014208:Treponema
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S