title: Comparative histological study on wound healing on rat's skin treated with Mitomycin C or Clobetasol propionate.
abstract: OBJECTIVE
  To compare histologically the action of Mitomycin C and that of Clobetasol propionate for surgical wound healing in rats.
  
  
  METHODS
  A circular skin fragment was surgically removed from 57 Wistar rats. Twenty-two animals were treated with Mitomycin C with topical medication in a single dose, 22 with Clobetasol propionate with a cream medication once a day for 15 days and 13 did not receive any medication. The animals were euthanized 30 and 60 days, and the scars subjected to histological examination.
  
  
  RESULTS
  The histological analysis on the samples did not show statistically significant differences regarding the quantities of fibroblasts, fibrocytes and vascular proliferation in the three groups, in the evaluations after 30 and 60 days. In the treated groups with Mitomycin C and Clobetasol there was a decrease in collagen concentration over the 30-day period and an increase in collagen concentration over the 60-day period, in comparison with the control group.
  
  
  CONCLUSIONS
  The actions of Mitomycin C and Clobetasol were equivalent and not interfere in fibroplasias and in angiogenesis. Both drugs initially cause a decrease in collagen over a 30-day period and an increase over a 60-day period, demonstrating a delay in the wound healing.
authors.affiliation: Department of Morphology, Santa Casa School of Medical Sciences of Sao Paulo, Universidade de Sao Paulo, BR.
authors.forename: Maria de Fátima Pereira de
authors.initials: Mde F
authors.lastname: Carvalho
authors.affiliation: Department of Otorhinolaryngology, Santa Casa School of Medical Sciences of Sao Paulo, USP, Sao Paulo, SP, BR.
authors.forename: Celina Siqueira Barbosa
authors.initials: CS
authors.lastname: Pereira
authors.affiliation: Research and Teaching Institute, Hospital de Câncer de Barretos, BR.
authors.forename: José Humberto
authors.initials: JH
authors.lastname: Fregnani
authors.affiliation: Department of Morphology, Santa Casa School of Medical Sciences of Sao Paulo, USP, Sao Paulo, SP, BR.
authors.forename: Fernando de Andrade Quintanilha
authors.initials: Fde A
authors.lastname: Ribeiro
mesh_terms: D000287:Administration, Topical; D000477:Alkylating Agents / Q000627:therapeutic use*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002990:Clobetasol / Q000627:therapeutic use*; D003094:Collagen / Q000032:analysis / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005938:Glucocorticoids / Q000627:therapeutic use*; D008297:Male; D016685:Mitomycin / Q000627:therapeutic use*; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000477:Alkylating Agents; D005938:Glucocorticoids; D016685:Mitomycin; D003094:Collagen; D002990:Clobetasol