title: Fabrication of Gold Nanoparticles/Graphene-PDDA Nanohybrids for Bio-detection by SERS Nanotechnology.
abstract: In this research, graphene nanosheets were functionalized with cationic poly (diallyldimethylammonium chloride) (PDDA) and citrate-capped gold nanoparticles (AuNPs) for surface-enhanced Raman scattering (SERS) bio-detection application. AuNPs were synthesized by the traditional citrate thermal reduction method and then adsorbed onto graphene-PDDA nanohybrid sheets with electrostatic interaction. The nanohybrids were subject to characterization including X-ray diffraction (XRD), transmission electron microscopy (TEM), zeta potential, and X-ray photoelectron spectroscopy (XPS). The results showed that the diameter of AuNPs is about 15-20 nm immobilized on the graphene-PDDA sheets, and the zeta potential of various AuNPs/graphene-PDDA ratio is 7.7-38.4 mV. Furthermore, the resulting nanohybrids of AuNPs/graphene-PDDA were used for SERS detection of small molecules (adenine) and microorganisms (Staphylococcus aureus), by varying the ratios between AuNPs and graphene-PDDA. AuNPs/graphene-PDDA in the ratio of AuNPs/graphene-PDDA = 4:1 exhibited the strongest SERS signal in SERS detection of adenine and S. aureus. Thus, it is promising in the application of rapid and label-free bio-detection of bacteria or tumor cells.
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan. D10104806@mail.ntust.edu.tw.
authors.forename: Andreas H H
authors.initials: AH
authors.lastname: Mevold
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan. mazerious@gmail.com.
authors.forename: Wei-Wu
authors.initials: WW
authors.lastname: Hsu
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan. d10004810@mail.ntust.edu.tw.
authors.forename: Andri
authors.initials: A
authors.lastname: Hardiansyah
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan. lyhuang0123@gmail.com.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Huang
authors.affiliation: Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, 106, Taiwan. myang@mail.ntust.edu.tw.
authors.forename: Ming-Chien
authors.initials: MC
authors.lastname: Yang
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. tyliu0322@gmail.com.
authors.forename: Ting-Yu
authors.initials: TY
authors.lastname: Liu
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. u01187039@mail2.mcut.edu.tw.
authors.forename: Tzu-Yi
authors.initials: TY
authors.lastname: Chan
authors.affiliation: Department of Materials Engineering, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. u01187102@mail2.mcut.edu.tw.
authors.forename: Kuan-Syun
authors.initials: KS
authors.lastname: Wang
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 106, Taiwan. kizukisu@gmail.com.
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Su
authors.affiliation: Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 106, Taiwan. rujong@ntu.edu.tw.
authors.forename: Ru-Jong
authors.initials: RJ
authors.lastname: Jeng
authors.affiliation: Center for Condensed Matter Sciences, National Taiwan University, Taipei, 10617, Taiwan. jkwang@ntu.edu.tw.|Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 10617, Taiwan. jkwang@ntu.edu.tw.
authors.forename: Juen-Kai
authors.initials: JK
authors.lastname: Wang
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 10617, Taiwan. ylwang@pub.iams.sinica.edu.tw.|Department of Physics, National Taiwan University, Taipei, 10617, Taiwan. ylwang@pub.iams.sinica.edu.tw.
authors.forename: Yuh-Lin
authors.initials: YL
authors.lastname: Wang
keywords: Bio-detection; Gold nanoparticles; Graphene; Surface-enhanced Raman scattering