title: The occurrence of cervical cancer in Uygur women in Xinjiang Uygur Autonomous Region is correlated to microRNA-146a and ethnic factor.
abstract: OBJECTIVE
  The present study is to investigate the effect of microRNA-146a (miR-146a) and ethnic factor in the occurrence of cervical cancer in Uygur women in Xinjiang Uygur Autonomous Region.
  
  
  METHODS
  A total of 620 pieces of cervical tissues were obtained between September 2010 and September 2013, including 208 cases of cervicitis, 207 cases of cervical intraepithelial neoplasia, and 205 cases of cervical cancer. The relative expression of miR-146a in tissues was measured using quantitative real-time polymerase chain reaction. Polymerase chain reaction - restriction fragment length polymorphism was used to determine the genotypes of miR-146a (rs2910164). Differences between two groups and multiple groups were compared using t-test and one-factor analysis of variance, respectively. Comparison of genotype compositions and genetic balance examinations were performed using χ(2) test.
  
  
  RESULTS
  Uygur women had earlier age of marriage, more times of pregnancy, and more childbirths than Han women. The miR-146a (rs2910164) genotype composition was significantly different between Uygur and Han, with the ratio of GG genotype in Uygur being higher than that in Han. Logistic regression analysis showed that miR-146a (rs2910164) genotypes were significantly correlated to ethnic factor and tumor sizes. The expression of miR-146a was elevated in cervical intraepithelial neoplasia and cervical cancer, especially for Uygur women, with the GG genotype being the most highly expressed.
  
  
  CONCLUSIONS
  The miR-146a (rs2910164) polymorphism is significantly correlated to ethnic factor and tumor diameters. miR-146a has differential expression in cervical tissues. Allele G of miR-146a (rs2910164) is related to the high expression of miR-146a, and the progression of cervical cancer.
authors.affiliation: Department of Gynecology, State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University Urumqi, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University Urumqi, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Pathology, The First Affiliated Hospital of Xinjiang Medical University Urumqi, P. R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Gynecology, State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University Urumqi, P. R. China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gynecology, State Key Lab Incubation Base of Xinjiang Major Diseases Research, The First Affiliated Hospital of Xinjiang Medical University Urumqi, P. R. China.
authors.forename: Cailing
authors.initials: C
authors.lastname: Ma
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005006:Ethnicity; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D002583:Uterine Cervical Neoplasms / Q000208:ethnology* / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000208:ethnology* / Q000235:genetics
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs
keywords: Cervical cancer; Uygur; microRNA-146a; rs2910164