title: Secondary bile acids effects in colon pathology. Experimental mice study.
abstract: OBJECTIVE
  To assess whether deoxycholic acid (DOC) and lithocholic acid (LCA) administered in a period of six months in a concentration of 0.25% may have a carcinogenic role in mice colon.
  
  
  METHODS
  The study used C57BL6 female mice divided into four groups. The control group received a balanced diet and the others received diets supplemented with 0.25% DOC, 0.25% LCA and 0.125% DOC+0.125% LCA, respectively. After euthanasia, the lesions found in the resected gastrointestinal tracts were stained with hematoxylin-eosin and examined microscopically.
  
  
  RESULTS
  No gastrointestinal tract changes were observed in the control group, while hyperplastic Peyer's patches in the small intestine, flat adenomas with mild dysplasia and chronic colitis at the level of the colon were found in all three test groups. The colonic lesions prevailed in the proximal colon. The highest number of flat adenoma lesions (8), hyperplasia of Peyer's patches (25) and chronic colitis (2) were found in mice fed with diet and LCA.
  
  
  CONCLUSIONS
  Precancerous or cancerous pathological lesions could not be identified. Instead, adenomatous colonic injuries occurred in a shorter period of time (six months), compared to the reported data.
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Claudiu
authors.initials: C
authors.lastname: Saracut
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Calin
authors.initials: C
authors.lastname: Molnar
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Cristian
authors.initials: C
authors.lastname: Russu
authors.affiliation: Department of Pharmaceutical Technology, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Todoran
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Medicine and Pharmacy Cluj Napoca, RO.
authors.forename: Laurian
authors.initials: L
authors.lastname: Vlase
authors.affiliation: Department of Pathology, University of Medicine and Pharmacy Cluj Napoca, RO.
authors.forename: Sabin
authors.initials: S
authors.lastname: Turdean
authors.affiliation: Department of Epidemiology, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Septimiu
authors.initials: S
authors.lastname: Voidazan
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy Tg-Mures, RO.
authors.forename: Constantin
authors.initials: C
authors.lastname: Copotoiu
mesh_terms: D000236:Adenoma / Q000139:chemically induced; D000818:Animals; D001647:Bile Acids and Salts / Q000633:toxicity*; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D002756:Cholagogues and Choleretics / Q000633:toxicity*; D003092:Colitis / Q000139:chemically induced; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced; D003840:Deoxycholic Acid / Q000633:toxicity*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry; D005260:Female; D008095:Lithocholic Acid / Q000633:toxicity*; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000187:drug effects; D013997:Time Factors
chemical_list: D001647:Bile Acids and Salts; D002273:Carcinogens; D002756:Cholagogues and Choleretics; D003840:Deoxycholic Acid; D008095:Lithocholic Acid