title: DNA Vaccines: Experiences in the Swine Model.
abstract: DNA vaccination is one of the most fascinating vaccine-strategies currently in development. Two of the main advantages of DNA immunization rely on its simplicity and flexibility, being ideal to dissect both the immune mechanisms and the antigens involved in protection against a given pathogen. Here, we describe several strategies used to enhance the immune responses induced and the protection afforded by experimental DNA vaccines tested in swine and provide with very basic protocol describing the generation and in vivo application of a prototypic DNA vaccine. Only time will tell the last word regarding the definitive implementation of DNA vaccination in the field.
authors.affiliation: UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain. francesc.accensi@uab.cat.|Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, Bellaterra, Barcelona, 08193, Spain. francesc.accensi@uab.cat.
authors.forename: Francesc
authors.initials: F
authors.lastname: Accensi
authors.affiliation: IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rodríguez
authors.affiliation: IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
authors.forename: Paula L
authors.initials: PL
authors.lastname: Monteagudo
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D017951:Antigen Presentation / Q000276:immunology*; D013552:Swine / Q000276:immunology / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000906:Antibodies; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: APC; Antibodies; Antigen presentation; Cytotoxic T-cell responses (CTL); DNA vaccine; ELI; Electroporation; Genetic adjuvant; Swine; Veterinary virology