title: Fluorescent labeling reliably identifies Chlamydia trachomatis in living human endometrial cells and rapidly and accurately quantifies chlamydial inclusion forming units.
abstract: Chlamydia replication requires host lipid acquisition, allowing flow cytometry to identify Chlamydia-infected cells that accumulated fluorescent Golgi-specific lipid. Herein, we describe modifications to currently available methods that allow precise differentiation between uninfected and Chlamydia trachomatis-infected human endometrial cells and rapidly and accurately quantify chlamydial inclusion forming units.
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA. Electronic address: vicettimiguel.1@osu.edu.
authors.forename: Rodolfo D
authors.initials: RD
authors.lastname: Vicetti Miguel
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Henschel
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
authors.forename: Fiorela C
authors.initials: FC
authors.lastname: Dueñas Lopez
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
authors.forename: Nirk E
authors.initials: NE
authors.lastname: Quispe Calla
authors.affiliation: Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH 43210, USA; Department of Obstetrics & Gynecology, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Cherpes
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000254:growth & development*; D004717:Endometrium / Q000166:cytology / Q000382:microbiology*; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes
keywords: Ceramide; Chlamydia trachomatis; Flow cytometry; Human endometrial cells; Inclusion forming units