title: Whole blood EBV-DNA predicts outcome in diffuse large B-cell lymphoma.
abstract: An association between Epstein-Barr Virus (EBV) infection and lymphoproliferative diseases has been reported with EBV + diffuse large B cell-lymphoma (DLBCL) of the elderly described as a distinct entity. In a cohort of 218 human immunodeficiency virus (HIV)-negative patients with diffuse large B-cell lymphomas, we detected EBV-DNA in 25% of whole blood (WB) samples at diagnosis. Presence and viral load in WB, mononuclear cells or plasma did not predict the presence of EBV in the tumor biopsy. Positive Hepatitis C virus (HCV) serology was associated with a higher frequency of EBV in WB. Patients with EBV-DNA in WB had a significantly shorter progression-free (p = 0.02) and overall survival (p = 0.05) after immunochemotherapy with R-CHOP (Rituximab, Cyclophosphamide, Doxorubicin, Vincristine, Prednisolone). We conclude that detection of EBV in WB is not a surrogate marker for EBV-association in diffuse large B-cell lymphoma, however it associates with worse outcome.
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Tisi
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Elisa
authors.initials: E
authors.lastname: Cupelli
authors.affiliation: b Institutes of Microbiology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Rosaria
authors.initials: R
authors.lastname: Santangelo
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Elena
authors.initials: E
authors.lastname: Maiolo
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Eleonora
authors.initials: E
authors.lastname: Alma
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Manuela
authors.initials: M
authors.lastname: Giachelia
authors.affiliation: c Institutes of Pathological Anatomy , Catholic University S. Cuore , Rome , Italy , and.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Martini
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Silvia
authors.initials: S
authors.lastname: Bellesi
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Alò
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Voso
authors.affiliation: d Department of Internal Medicine , Catholic University S. Cuore , Rome , Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pompili
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Leone
authors.affiliation: c Institutes of Pathological Anatomy , Catholic University S. Cuore , Rome , Italy , and.
authors.forename: Luigi Maria
authors.initials: LM
authors.lastname: Larocca
authors.affiliation: a Institutes of Hematology , Catholic University S. Cuore , Rome , Italy .
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohaus
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D058846:Antibodies, Monoclonal, Murine-Derived / Q000627:therapeutic use; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D003520:Cyclophosphamide / Q000627:therapeutic use; D004279:DNA, Viral; D004317:Doxorubicin / Q000627:therapeutic use; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000276:immunology / Q000821:virology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology* / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011241:Prednisone / Q000627:therapeutic use; D011379:Prognosis; D000069283:Rituximab; D016896:Treatment Outcome; D014750:Vincristine / Q000627:therapeutic use; D019562:Viral Load; D055815:Young Adult
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D004279:DNA, Viral; C571759:R-CHOP protocol; D000069283:Rituximab; D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D011241:Prednisone
keywords: Diffuse large B-cell lymphoma; EBV; prognosis