title: Effect of Surface Chemical Functionalities on Collagen Deposition by Primary Human Dermal Fibroblasts.
abstract: Surface modification has been identified as an important technique that could improve the response of the body to implanted medical devices. Collagen production by fibroblasts is known to play a vital role in wound healing and device fibrous encapsulation. However, how surface chemistry affects collagen I and III deposition by these cells has not been systematically studied. Here, we report how surface chemistry influences the deposition of collagen I and III by primary human dermal fibroblasts. Amine (NH3), carboxyl acid (COOH), and hydrocarbon (CH3) surfaces were generated by plasma deposition. This is a practically relevant tool to deposit a functional coating on any type of substrate material. We show that fibroblasts adhere better and proliferate faster on amine-rich surfaces. In addition, the initial collagen I and III production is greater on this type of coating. These data indicates that surface modification can be a promising route for modulating the rate and level of fibrous encapsulation and may be useful in informing the design of implantable biomedical devices to produce more predictable clinical outcomes.
authors.forename: Akash
authors.initials: A
authors.lastname: Bachhuka
authors.affiliation: Experimental Therapeutics Laboratory, Sansom Institute and Hanson Institute, School of Pharmacy and Medical Science, University of South Australia , Adelaide, South Australia 5000, Australia.
authors.forename: John
authors.initials: J
authors.lastname: Hayball
authors.forename: Louise E
authors.initials: LE
authors.lastname: Smith
authors.forename: K
authors.initials: K
authors.lastname: Vasilev
mesh_terms: D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D024061:Collagen Type III / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D010949:Plasma / Q000737:chemistry; D012867:Skin / Q000737:chemistry; D013499:Surface Properties / Q000187:drug effects; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III
keywords: collagen I; collagen III; fibroblasts; plasma polymerization; surface chemistry