title: Catechin-7-O-β-D-glucopyranoside isolated from the seed of Phaseolus calcaratus Roxburgh ameliorates experimental colitis in rats.
abstract: The seeds of Phaseolus calcaratus Roxburgh (PHCR) are common legumes that comprise part of the daily diet in Chinese and Korean culture. Recent findings highlight anti-inflammatory and anti-septic potentials of catechin-7-O-β-D-glucopyranoside (CGP) isolated from PHCR seeds. We investigated the intestinal anti-inflammatory activity and associated mechanisms of CGP using a rat model of trinitrobenzenesulfonic acid (TNBS)-induced colitis. Oral treatment with CGP (10mg/kg body weight) suppressed body weight loss and intestinal inflammatory damages in TNBS-induced colitic rats. This treatment reduced myeloperoxidase activity and malondialdehyde level, but increased glutathione level in the TNBS colitic rats. CGP treatment also inhibited the TNBS-mediated increases in nitric oxide synthase, cyclooxygenase-2, interleukin-1β, tumor necrosis factor-α, intercellular adhesion molecule-1, and monocyte chemotactic protein-1 proteins or mRNA levels. This inhibition was accompanied by the increased mRNA levels of mucins MUC2 and MUC3. The CGP treatment prevented phosphorylation of p38 mitogen-activated protein kinase, IκB-α, and DNA-nuclear factor-κB binding, all of which were increased in the inflamed colons of TNBS-treated rats. Furthermore, oral administration with a crude PHCR butanol extract (100mg/kg body weight) which contains 1.5% of CGP showed intestinal anti-inflammatory potentials similar to that of CGP. Collectively, our current findings suggest that CGP or CGP-containing PHCR seeds may have favorable effects on intestinal inflammatory diseases.
authors.affiliation: Department of Bioactive Material Sciences, Institute of Molecular Biology and Genetics, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju 561-756, South Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Kook
authors.affiliation: Grassland and forage division, National Institute of Animal Science, RDA, Cheonan 330-801, South Korea.
authors.forename: Ki Choon
authors.initials: KC
authors.lastname: Choi
authors.affiliation: Department of Pharmaceutical Engineering, Konyang University, Nonsan 320-711, South Korea.
authors.forename: Seong-Wan
authors.initials: SW
authors.lastname: Cho
authors.affiliation: Center for Health Care Technology Development, HanPoong Pharmaceutical Co. Ltd., Jeonju 561-201, South Korea.
authors.forename: Hyoung-Kwon
authors.initials: HK
authors.lastname: Cho
authors.affiliation: Department of Oriental Medicine Materials, Dongsin University, Naju 520-714, South Korea.
authors.forename: Kyung Dong
authors.initials: KD
authors.lastname: Lee
authors.affiliation: Department of Bioactive Material Sciences, Institute of Molecular Biology and Genetics, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju 561-756, South Korea. Electronic address: leejc88@jbnu.ac.kr.
authors.forename: Jeong-Chae
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000627:therapeutic use; D003424:Crohn Disease / Q000139:chemically induced* / Q000188:drug therapy*; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000302:isolation & purification / Q000627:therapeutic use*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009077:Mucins / Q000096:biosynthesis; D009195:Peroxidase / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005960:Glucosides; D009077:Mucins; D012333:RNA, Messenger; C556021:catechin 7-O-glucopyranoside; D008315:Malondialdehyde; D002392:Catechin; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D005978:Glutathione
keywords: Catechin-7-O-β-d-glucopyranoside; Intestinal anti-inflammation; Phaseolus calcaratus R; Ulcerative colitis