title: Electrochemical monitoring of the interaction between Temozolamide and nucleic acids by using disposable pencil graphite electrodes.
abstract: Temozolomide (TMZ) is an anticancer drug used for the treatment of adult brain tumour and skin cancer. The biomolecular interaction between TMZ and DNA was investigated for the first time in this study using disposable pencil graphite electrodes (PGEs) in combination with electrochemical techniques. The surface confined interactions between TMZ and different type of nucleic acids were performed. Before/after surface confined interaction process, the oxidation signals of TMZ, guanine and adenine were measured using differential pulse voltammetry (DPV) and PGE and accordingly, the changes at the oxidation signals were evaluated. The detection limit (DL) was also estimated based on the oxidation signal of TMZ. The interaction of TMZ with single stranded poly [A], poly [G], or double stranded poly [A]-poly[T] and poly [G]-poly[C] was also explored. Moreover, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were utilized for detection the interaction between TMZ and DNA. The features of this single-use electrochemical sensor was discussed in comparison to other reports that were developed for TMZ detection.
authors.affiliation: Faculty Of Pharmacy, Analytical Chemistry Department, Ege University, 35100 Bornova, Izmir, Turkey; The Institute Of Natural And Applied Sciences, Biomedical Technologies Department, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Altay
authors.affiliation: Faculty Of Pharmacy, Analytical Chemistry Department, Ege University, 35100 Bornova, Izmir, Turkey; The Institute Of Natural And Applied Sciences, Biotechnology Department, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Eksin
authors.affiliation: Faculty Of Pharmacy, Analytical Chemistry Department, Ege University, 35100 Bornova, Izmir, Turkey; The Institute Of Natural And Applied Sciences, Biotechnology Department, Ege University, 35100 Bornova, Izmir, Turkey.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Congur
authors.affiliation: Faculty Of Pharmacy, Analytical Chemistry Department, Ege University, 35100 Bornova, Izmir, Turkey; The Institute Of Natural And Applied Sciences, Biomedical Technologies Department, Ege University, 35100 Bornova, Izmir, Turkey; The Institute Of Natural And Applied Sciences, Biotechnology Department, Ege University, 35100 Bornova, Izmir, Turkey. Electronic address: arzum.erdem@ege.edu.tr.
authors.forename: Arzum
authors.initials: A
authors.lastname: Erdem
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism*; D003362:Cost-Benefit Analysis; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004209:Disposable Equipment; D004563:Electrochemistry / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D000077204:Temozolomide
chemical_list: D000970:Antineoplastic Agents; D006108:Graphite; D003606:Dacarbazine; D004247:DNA; D000077204:Temozolomide
keywords: Cyclic voltammetry; DNA biosensor; Differential pulse voltammetry; Electrochemical impedance spectroscopy; Pencil graphite electrode; Temozolomide