title: Construction and Application of Newcastle Disease Virus-Based Vector Vaccines.
abstract: Paramyxoviruses are able to stably express a wide-variety of heterologous antigens at relatively high levels in various species and are consequently considered as potent gene delivery vehicles. A single vaccination is frequently sufficient for the induction of robust humoral and cellular immune responses. Here we provide detailed methods for the construction and application of Newcastle disease virus (NDV)-based vector vaccines. The in silico design and in vitro rescue as well as the in vivo evaluation of NDV based vectors are described in this chapter.
authors.affiliation: Department of Virology, Central Veterinary Institute, Wageningen University and Research Centre, Houtribweg 39, Lelystad, 8221 RA, The Netherlands. paul.wichgersschreur@wur.nl.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Wichgers Schreur
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology; D003198:Computer Simulation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007111:Immunity, Cellular / Q000235:genetics* / Q000276:immunology; D009521:Newcastle Disease / Q000517:prevention & control* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D011200:Poultry / Q000821:virology; D059386:Reverse Genetics; D014611:Vaccination / Q000379:methods* / Q000662:veterinary
chemical_list: D015478:Antigens, Heterophile
keywords: LaSota; Lentogenic; Newcastle disease virus; Paramyxovirus; Reverse genetics; Vaccine; Vector