title: Investigation of plant latices of Asteraceae and Campanulaceae regarding proteolytic activity.
abstract: Occurrence of plant latices is widespread, there are more than 40 families of plants characterized to establish lactiferous structures. The appearance of hydrolytic active proteins, incorporated in latices is already characterized, and hydrolytic active proteins are considerable, and for several plant families, the occurrence of hydrolytic active proteins is already specified e.g. Apocynaceae Juss., Caricaceae Dumort, Euphorbiaceae Juss., Moraceae Gaudich and Papaveraceae Juss. In our investigation, focused on latex bearing plants of order Asterales, Asteraceae and Campanulaceae in particular. The present outcomes represent a comprehensive study, relating to the occurrence of proteolytic active enzymes of order Asterales for the first time. 131 different species of Asteraceae and Campanulaceae were tested, and the appearance of plant latex proteases were determined in different quantities. Proteolytic activity was investigated by inhibitory studies and determination of residual activity in the following, enable us to characterize the proteases. Most of the considered species exhibit a serine protease activity and a multiplicity of species exhibited two or more subclasses of proteases.
authors.affiliation: Institute of Pharmacy, Freie Universitaet, Berlin, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Sytwala
authors.affiliation: Institute of Pharmacy, Freie Universitaet, Berlin, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Domsalla
authors.affiliation: Institute of Pharmacy, Freie Universitaet, Berlin, Germany. Electronic address: melzig@zedat.fu-berlin.de.
authors.forename: Matthias F
authors.initials: MF
authors.lastname: Melzig
mesh_terms: D019659:Asteraceae / Q000201:enzymology*; D029422:Campanulaceae / Q000201:enzymology*; D007840:Latex / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism
chemical_list: D007840:Latex; D010940:Plant Proteins; D010447:Peptide Hydrolases
keywords: Asteraceae; Campanulaceae; Plant latex; Proteolytic activity