title: Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability.
authors.affiliation: Université de Lorraine, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; INRA, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France.
authors.forename: Annabelle
authors.initials: A
authors.lastname: Thibessard
authors.affiliation: Université de Lorraine, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; INRA, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Bertrand
authors.affiliation: Université de Lorraine, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; INRA, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; Ecole Polytechnique Fédérale de Lausanne, Laboratory of Protein Engineering, EPFL SB ISIC LIP1, BCH 4303 (Bât. BCH), CH-1015 Lausanne, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Hiblot
authors.affiliation: Université de Lorraine, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; INRA, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Piotrowski
authors.affiliation: Université de Lorraine, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France; INRA, Dynamique des Génomes et Adaptation Microbienne, UMR 1128, Vandœuvre-lès-Nancy, F-54506, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Leblond
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D003227:Conjugation, Genetic / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D018628:Gene Dosage / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013302:Streptomyces / Q000235:genetics*; D013883:Thiostrepton / Q000096:biosynthesis; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015534:Trans-Activators; C485184:enhanced green fluorescent protein; C089891:tipA protein, Streptomyces; D049452:Green Fluorescent Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; D013883:Thiostrepton
keywords: Cloning; Escherichia coli; Streptomyces; Toxic; tipA promoter