title: U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA.
abstract: We studied the intramitochondrial localization of several multiprotein complexes involved in U-insertion/deletion RNA editing in trypanosome mitochondria. The editing complexes are located in one or two antipodal nodes adjacent to the kinetoplast DNA (kDNA) disk, which are distinct from but associated with the minicircle catenation nodes. In some cases the proteins are in a bilateral sheet configuration. We also found that mitoribosomes have a nodal configuration. This type of organization is consistent with evidence for protein and RNA interactions of multiple editing complexes to form an ~40S editosome and also an interaction of editosomes with mitochondrial ribosomes.
authors.affiliation: Department of Gerontology, Davis School of Gerontology, University of Southern California, Los Angeles, CA 90089, United States; Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Wong
authors.affiliation: Multispan Inc., Hayward, CA 94544, United States; Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States.
authors.forename: Katelynn
authors.initials: K
authors.lastname: Kazane
authors.affiliation: Department of Biology, University of California - Riverside, Riverside, CA 92521, United States.
authors.forename: Dmitri A
authors.initials: DA
authors.lastname: Maslov
authors.affiliation: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA 92521, United States.
authors.forename: Kestrel
authors.initials: K
authors.lastname: Rogers
authors.affiliation: Department of Molecular and Cell Biology, Boston University School of Dental Medicine, Boston, MA 02118, United States.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Aphasizhev
authors.affiliation: Department of Microbiology, Immunology and Molecular Genetics, Geffen School of Medicine at UCLA, Los Angeles, CA 90095, United States. Electronic address: larrys3255@gmail.com.
authors.forename: Larry
authors.initials: L
authors.lastname: Simpson
mesh_terms: D018105:DNA, Kinetoplast / Q000378:metabolism*; D007891:Leishmania / Q000201:enzymology* / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D000069396:Mitochondrial Ribosomes / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D017393:RNA Editing
chemical_list: D018105:DNA, Kinetoplast; D046912:Multiprotein Complexes
keywords: Antipodal node; Editing; Kinetoplast; Leishmania; RNA; Trypanosome