title: Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner.
abstract: Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various cells. Poly(P) concentrations of 1 and 0.5 mg/mL yielded high levels of mineralization in ROS17/2.8 osteoblast cells. Similarly, Poly(P) induced mineralization in cell types expressing alkaline phosphatase (ALP), namely, ATDC5 and MC3T3-E1, but not in CHO, C3H10T1/2, C2C12, and 3T3-L1 cells. Furthermore, forced expression of ALP caused Poly(P)-induced mineralization in CHO cells. These results suggest that ALP determines Poly(P)-induced mineralization in a cell-type independent manner.
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Mikami
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan. tsuda.hiromasa@nihon-u.ac.jp.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tsuda
authors.affiliation: Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Akiyama
authors.affiliation: Department of Oral Anatomy, Aichi-Gakuin University School of Dentistry, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Honda
authors.affiliation: Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Shimizu
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komiyama
mesh_terms: D041721:3T3-L1 Cells; D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D016466:CHO Cells; D002113:Calcification, Physiologic / Q000187:drug effects* / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009928:Organ Specificity / Q000187:drug effects / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*; D011122:Polyphosphates / Q000494:pharmacology*; D051381:Rats
chemical_list: D011122:Polyphosphates; D000469:Alkaline Phosphatase
keywords: Alkaline phosphatase; Mineralization; Polyphosphate