title: [Tumor angiogenesis promoted by fusion of glioma stem/progenitor cells with bone marrow mesenchymal stem cells].
abstract: OBJECTIVE
  The aim of this study was to clarify whether the fusion of bone marrow mesenchymal stem cells (MSCs) with tumor cells can promote tumor angiogensis.
  
  
  METHODS
  Human glioma stem/progenitor cells (GSPCs) (SU3 cells) were transfected with red fluorescent protein (RFP) gene. Bone marrow mesenchymal stem cells (MSCs) were harvested from nude mice with whole-body green fluorescent protein (GFP) gene expression. Then the two kinds of cells were co-cultured in vitro. At the same time SU3-RFP was transplanted into the brain of GFP-expressing nude mice to establish xenograft tumors. The co-cultured cells, GFP/RFP double positive (yellow) cells and blood vessels obtained from the xenograft tumors were observed under fluorescent microscope and laser scanning confocal microscope.
  
  
  RESULTS
  After five passages in vitro, MSCs maintained the proliferative activity and highly expressed CD105. CD105 was also expressed in the femurs of GFP-expressing nude mice, tumor cells, blood vessels of SU3 xenograft tumors, and clinical malignant gliomas. When MSCs were co-cultured with SU3-RFP, the ratio of yellow cells co-expressing RFP and GFP was significantly increased after extended time and continuous passages. According to the flow cytometry, yellow cells co-expressing RFP and GFP were 83.7% of the cultured cells. In tissue slices of the xenograft tumors, bundles of yellow vessel-like structure and cross-sectioned yellow vascular wall structures including vascular wall stroma cells were observed with RFP and GFP expression, and were identified as de novo formed vessels derived from fusion of MSCs with SU3-RFP cells.
  
  
  CONCLUSIONS
  Cell fusion occurs between tumor cells and host MSCs and it promotes tumor angiogenesis.
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Zhao
authors.forename: Xingliang
authors.initials: X
authors.lastname: Dai
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoci
authors.initials: X
authors.lastname: Rong
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.forename: Qilong
authors.initials: Q
authors.lastname: Wang
authors.forename: Qin
authors.initials: Q
authors.lastname: Rui
authors.forename: Aidong
authors.initials: A
authors.lastname: Wang
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Email: szlq006@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology*; D002450:Cell Communication; D002459:Cell Fusion; D002478:Cells, Cultured; D005910:Glioma; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins; D059630:Mesenchymal Stem Cells; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009369:Neoplasms; D009389:Neovascularization, Pathologic; D013234:Stem Cells; D014162:Transfection; D014183:Transplantation, Heterologous; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein