title: A fast isocratic liquid chromatography method for the quantification of xanthophylls and their stereoisomers.
abstract: A fast isocratic liquid chromatography method was developed for the simultaneous quantification of eight xanthophylls (13-Z-lutein, 13'-Z-lutein, 13-Z-zeaxanthin, all-E-lutein, all-E-zeaxanthin, all-E-canthaxanthin, all-E-β-apo-8'-carotenoic acid ethyl ester and all-E-β-apo-8'-carotenal) within 12 min, compared to 90 min by the conventional high-performance liquid chromatography method. The separation was achieved on a YMC C30 reversed-phase column (100 mm x 2.0 mm; 3 μm) operated at 20°C using a methanol/tert-butyl methyl ether/water solvent system at a flow rate of 0.8 mL/min. The method was successfully applied to quantify lutein and zeaxanthin stereoisomers in egg yolk, raw and cooked spinach, and a dietary supplement. The method can be used for the rapid analysis of xanthophyll isomers in different food products and for quality control purposes.
authors.affiliation: Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Chamila
authors.initials: C
authors.lastname: Nimalaratne
authors.affiliation: Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Daise
authors.initials: D
authors.lastname: Lopes-Lutz
authors.affiliation: Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, Edmonton, Alberta, Canada.|Department of Nutritional and Food Sciences, University of Bonn, Bonn, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schieber
authors.affiliation: Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D002853:Chromatography, Liquid; D019587:Dietary Supplements / Q000032:analysis; D004530:Egg Yolk / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D018724:Spinacia oleracea / Q000737:chemistry; D013237:Stereoisomerism; D013997:Time Factors; D024341:Xanthophylls / Q000032:analysis* / Q000737:chemistry*
chemical_list: D024341:Xanthophylls
keywords: Geometric isomers; Isocratic elution; Xanthophylls