title: Differences in the Elemental Isotope Definition May Lead to Errors in Modern Mass-Spectrometry-Based Proteomics.
abstract: The elemental isotope definition used to calculate the theoretical masses and isotope distribution of (bio)molecules is considered to be a fixed, universal standard in mass-spectrometry-based proteomics. However, this is an incorrect assumption. In view of the ongoing advances in mass spectrometry technology, and in particular the ever-increasing mass precision, the elemental isotope definition and its variations should be taken into account. We illustrate the effect of the elemental isotope uncertainty on the theoretical and experimental masses with theoretical calculations and examples.
authors.affiliation: Interuniversity Institute of Biostatistics and statistical Bioinformatics, Hasselt University , 3590 Diepenbeek, Belgium.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Claesen
authors.forename: Frederik
authors.initials: F
authors.lastname: Lermyte
authors.forename: Frank
authors.initials: F
authors.lastname: Sobott
authors.affiliation: Interuniversity Institute of Biostatistics and statistical Bioinformatics, Hasselt University , 3590 Diepenbeek, Belgium.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Burzykowski
authors.affiliation: Interuniversity Institute of Biostatistics and statistical Bioinformatics, Hasselt University , 3590 Diepenbeek, Belgium.|Applied Bio and Molecular Systems, VITO , 2400 Mol, Belgium.
authors.forename: Dirk
authors.initials: D
authors.lastname: Valkenborg
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000032:analysis; D004602:Elements; D006736:Horses; D007553:Isotope Labeling; D007554:Isotopes; D013058:Mass Spectrometry; D008970:Molecular Weight; D009211:Myoglobin / Q000032:analysis; D040901:Proteomics / Q000592:standards*
chemical_list: D001059:Apoproteins; D004602:Elements; D007554:Isotopes; D009211:Myoglobin; C010857:apomyoglobin