title: 3-Bromopyruvate inhibits cell proliferation and induces apoptosis in CD133+ population in human glioma.
abstract: The study was aimed to investigate the role of 3-bromopyruvate in inhibition of CD133+ U87 human glioma cell population growth. The results demonstrated that 3-bromopyruvate inhibited the viability of both CD133+ and parental cells derived from U87 human glioma cell line. However, the 3-bromopyruvate-induced inhibition in viability was more prominent in CD133+ cells at 10 μM concentration after 48 h. Treatment of CD133+ cells with 3-bromopyruvate caused reduction in cell population and cell size, membrane bubbling, and degradation of cell membranes. Hoechst 33258 staining showed condensation of chromatin material and fragmentation of DNA in treated CD133+ cells after 48 h. 3-Bromopyruvate inhibited the migration rate of CD133+ cells significantly compared to the parental cells. Flow cytometry revealed that exposure of CD133+ cells to 3-bromopyruvate increased the cell population in S phase from 24.5 to 37.9 % with increase in time from 12 to 48 h. In addition, 3-bromopyruvate significantly enhanced the expression of Bax and cleaved caspase 3 in CD133+ cells compared to the parental cells. Therefore, 3-bromopyruvate is a potent chemotherapeutic agent for the treatment of glioma by targeting stem cells selectively.
authors.affiliation: Clinical Laboratory, Wendeng Central Hospital of Weihai, Weihai, Shandong, 264423, China.
authors.forename: Dong-Qiang
authors.initials: DQ
authors.lastname: Xu
authors.affiliation: Clinical Laboratory, Liaocheng People's Hospital of Taishan Medical University, 67 Dongchangxi Road, Dongchang District, Liaocheng, Shandong, 252000, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Tan
authors.affiliation: Clinical Laboratory, Yi Cheng District People's Hospital of Zao Zhuang, Zaozhuang, Shandong, 277300, China.
authors.forename: Bao-Wei
authors.initials: BW
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory, Yucheng People's Hospital, Yucheng, Shandong, 251200, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, 250021, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Clinical Laboratory, Liaocheng People's Hospital of Taishan Medical University, 67 Dongchangxi Road, Dongchang District, Liaocheng, Shandong, 252000, China. sunsjssh6@hotmail.com.
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Sun
authors.affiliation: Clinical Laboratory, Liaocheng People's Hospital of Taishan Medical University, 67 Dongchangxi Road, Dongchang District, Liaocheng, Shandong, 252000, China. caoyinguang63@hotmail.com.|Shandong University School of Medicine, Jinan, Shandong, 250012, China. caoyinguang63@hotmail.com.
authors.forename: Yin-Guang
authors.initials: YG
authors.lastname: Cao
mesh_terms: D000071916:AC133 Antigen / Q000032:analysis*; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D053148:Caspase 3 / Q000502:physiology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D011773:Pyruvates / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000502:physiology
chemical_list: D000071916:AC133 Antigen; C494826:BAX protein, human; C000606023:PROM1 protein, human; D011773:Pyruvates; D051028:bcl-2-Associated X Protein; C017092:bromopyruvate; D053148:Caspase 3
keywords: 3-Bromopyruvate; Apoptosis; Cell cycle; Glioma; Inhibition; Stem cells