title: [Quantity and function of regulatory B cells of the patients with immune thrombocytopenia].
abstract: OBJECTIVE
  To explore the quantity and function of regulatory B cells (Bregs) in peripheral blood (PB) of the patients with immune thrombocytopenia (ITP).
  
  
  METHODS
  A total of 67 ITP patients (35 patients were newly diagnosed, 32 patients were in remission) were collected from November 2013 to October 2014 in our hospital. And 30 normal controls were enrolled in this study. Their PB Bregs (CD19⁺CD24(hi)CD38(hi) cells) and related molecules interleukin-10 (IL-10) in Bregs were detected by flow cytometry (FCM). Correlation between Bregs and clinical parameters of ITP patients was analysed.
  
  
  RESULTS
  The percentage of Bregs in CD19⁺ cells of newly diagnosed ITP patients (3.09% ± 2.57%) was significantly lower than that of remitted ITP patients (7.78% ± 6.59%, P = 0.002) and controls (5.42% ± 3.31%, P = 0.003). The percentage of IL-10⁺ cells in Bregs of newly diagnosed ITP patients (32.30% ± 12.71%) was significantly lower than that of remitted ITP patients (71.73% ± 18.08%, P = 0.000) and controls (51.01% ± 17.08%, P = 0.026). And the percentage of IL-10⁺ cells in Bregs of remitted ITP patients was significantly higher than that of controls (P = 0.026). The level of Bregs of newly diagnosed ITP patients had significantly positive correlation with myeloid dendritic cells (mDC) (r = 0.644, P = 0.003) and PB platelet count (r = 0.327, P = 0.006).
  
  
  CONCLUSIONS
  Bregs involved in the pathogenesis of ITP might be a potential therapeutic target of this disease.
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China; Email: quwentj923@sina.com.
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Yihao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Shaoxue
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Huaquan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Guojin
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Xing
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Zonghong
authors.initials: Z
authors.lastname: Shao
mesh_terms: D060151:B-Lymphocytes, Regulatory; D003713:Dendritic Cells; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10; D016553:Purpura, Thrombocytopenic, Idiopathic
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10