title: Development of N-ferrocenyl(benzoyl)amino-acid esters stationary phase for high performance liquid chromatography.
abstract: A new stationary phase for high-performance liquid chromatography was prepared by covalently bonding N-ferrocenyl(benzoyl)amino-acid esters (L(1)) onto silica gel using 3-aminopropyltriethoxysilane as coupling reagent. The structure of new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. The chromatographic behavior of the phase was illustrated in reversed-phase (RP) and normal-phase (NP) modes using polycyclic aromatic hydrocarbons (PAHs), aromatics positional isomers, amines, 5-nitroimidazoles, organophosphorus pesticides and phenols as probes. Multiple mechanisms including hydrophobic, hydrogen bonding, π-π, dipole-dipole, charge-transfer and acid-base equilibrium interactions are involved. Thus, multi-interaction mechanisms and mixed-mode separation of the new phase can very likely guarantee its excellent chromatographic performance for the analysis of complex samples. The L(1) AminoSil column was successfully employed for the analysis of organophosphorus pesticides in vegetable.
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Metallurgical and Chemical Department, Jiyuan Vocational and Technical College, Jiyuan 459000, PR China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Li
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Cao
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Lijun
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: yuajuan@zzu.edu.cn.
authors.forename: Ajuan
authors.initials: A
authors.lastname: Yu
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: zsszz@126.com.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
authors.forename: Yangjie
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification*; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000596:Amino Acids; D004952:Esters; D009930:Organic Chemicals; D011437:Propylamines; D012821:Silanes; D012822:Silicon Dioxide; C477625:amino-propyl-triethoxysilane
keywords: Ferrocene stationary phase; High performance liquid chromatography; Organophosphorus pesticide; Retention mechanism