title: CXCR3 May Help Regulate the Inflammatory Response in Acute Lung Injury via a Pathway Modulated by IL-10 Secreted by CD8 + CD122+ Regulatory T Cells.
abstract: The aim of this study is to investigate the role of CXCR3 and IL-10 in lipopolysaccharide (LPS)-induced acute lung injury (ALI). ALI was induced by LPS injection (10 mg/kg) via the tail vein in C57BL/6 mice. Mice were sacrificed after 2 or 12 h to examine the levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) and histopathologic assessments. At 12 h after LPS injection, mice exhibited more severe lung infiltration by CD8+ T cell and less infiltration by CD8+CD122+ regulatory T cells than at 2 h after LPS challenge or in the control (mice not exposed to LPS). At 12 h, IFN-γ, CXCR3, and CXCL10 were significantly higher in the lungs. IL-10 in the lungs was significantly lower. CXCR3 may help to recruit CD8+ T cells and promotes IFN-γ and CXCL10 release. Such effects could be inhibited by IL-10 secreted by CD8+CD122+ regulatory T cells.
authors.affiliation: Department of Geriatrics, Renmin Hospital of Wuhan University, 238, Jiefang Road, Wucang, Wuhan, 430060, China. bywaters@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Nie
authors.affiliation: Clinical Laboratory Department, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Geriatrics, Renmin Hospital of Wuhan University, 238, Jiefang Road, Wucang, Wuhan, 430060, China.
authors.forename: Gangyan
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Geriatrics, Renmin Hospital of Wuhan University, 238, Jiefang Road, Wucang, Wuhan, 430060, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology / Q000473:pathology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D054357:Chemokine CXCL10 / Q000276:immunology* / Q000378:metabolism; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology* / Q000378:metabolism; D053646:Interleukin-2 Receptor beta Subunit / Q000378:metabolism*; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D054367:Receptors, CXCR3 / Q000276:immunology*
chemical_list: D054357:Chemokine CXCL10; C485433:Cxcl10 protein, mouse; C515188:Cxcr3 protein, mouse; C556062:IL10 protein, mouse; C508022:Il2rb protein, mouse; D053646:Interleukin-2 Receptor beta Subunit; D008070:Lipopolysaccharides; D054367:Receptors, CXCR3; D016753:Interleukin-10; D007371:Interferon-gamma
keywords: CD8 + CD122+ regulatory T cells; CXCR3; acute lung injury; interleukin-10