title: Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates.
abstract: The realization of polymer conjugate-based RNA delivery as a clinical modality requires the development and optimization of novel formulations. Although many literature examples of polymer conjugate-based SiRNA delivery systems exist, the protocols described herein represent a robust and facile way of screening any poly(amine)-based polymer system for SiRNA delivery. In this chapter, we describe the synthetic methods used to prepare poly(amide) polymers using a controlled polymerization method, as well as the preparation of the resulting targeted SiRNA polymer conjugates. In addition, detailed methods are provided for the characterization of the biodegradable poly(peptides) as well as the polymer conjugate that ensues.
authors.affiliation: Pharmaceutical Sciences and Clinical Supply, Merck Research Laboratories, 770 Sumneytown Pike, 4, West Point, PA, 19486, USA. stephanie_barrett@merck.com.
authors.forename: Stephanie E
authors.initials: SE
authors.lastname: Barrett
authors.affiliation: Discovery Chemistry, Merck Research Laboratories, 2000 Galloping Hill Road, Kenilworth, NJ, 07033, USA.
authors.forename: Erin N
authors.initials: EN
authors.lastname: Guidry
mesh_terms: D000588:Amines / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D009952:Ornithine / Q000737:chemistry; D010649:Phenylalanine / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000378:metabolism; D013388:Succinimides / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry
chemical_list: D000588:Amines; D004337:Drug Carriers; D009757:Nylons; D034741:RNA, Small Interfering; D013388:Succinimides; D013440:Sulfides; D011092:Polyethylene Glycols; D010649:Phenylalanine; C039011:N-hydroxysuccinimide S-acetylthioacetate; D009952:Ornithine
keywords: Biodegradable polymers; Polyamide; Polymer conjugates; SiRNA delivery; SiRNA protocols; Targeted delivery