title: Innate Reverse Transcriptase Activity of DNA Polymerase for Isothermal RNA Direct Detection.
abstract: RNA detection has become one of the most robust parts in molecular biology, medical diagnostics and drug discovery. Conventional RNA detection methods involve an extra reverse transcription step, which limits their further application for RNA rapid detection. We herein report a novel finding that Bst and Klenow DNA polymerases possess innate reverse transcriptase activities, so that the reverse transcription step and next amplification reaction can be combined to one step in isothermal RNA detection. We have demonstrated that Bst and Klenow DNA polymerases could be successfully used to reverse transcribe RNA within 125-nt length by real time RT-PCR and polyacrylamide gel electrophoresis (PAGE). Our findings will spur the development of a myriad of simple and easy to use RNA detection technologies for isothermal RNA direct detection. This will just meet the future needs of bioanalysis and clinical diagnosis to RNA rapid detection in POC settings and inspection and quarantine.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, PR China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Shen
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, PR China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Niu
authors.affiliation: Shandong Provincial Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology , Qingdao 266042, PR China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D012313:RNA / Q000032:analysis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013696:Temperature
chemical_list: D012313:RNA; D004259:DNA-Directed DNA Polymerase