title: Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.
abstract: The potential of RNA interference (RNAi)-based gene therapy has been demonstrated in many studies. However, clinical application of this technology has been hampered by a paucity of efficient and safe methods of delivering the RNAi activators. Prolonged transgene expression and improved safety of helper-dependent adenoviral vectors (HD AdVs) makes them well suited to delivery of engineered artificial intermediates of the RNAi pathway. Also, AdVs' natural hepatotropism makes them potentially useful for liver-targeted gene delivery. HD AdVs may be used for efficient delivery of cassettes encoding short hairpin RNAs and artificial primary microRNAs to the mouse liver. Methods for the characterization of HD AdV-mediated delivery of hepatitis B virus-targeting RNAi activators are described here.
authors.affiliation: Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Health Sciences Faculty, University of the Witwatersrand, Private Bag 3, Johannesburg, WITS 2050, South Africa.
authors.forename: Carol
authors.initials: C
authors.lastname: Crowther
authors.affiliation: Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Health Sciences Faculty, University of the Witwatersrand, Private Bag 3, Johannesburg, WITS 2050, South Africa.
authors.forename: Betty
authors.initials: B
authors.lastname: Mowa
authors.affiliation: Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Health Sciences Faculty, University of the Witwatersrand, Private Bag 3, Johannesburg, WITS 2050, South Africa. Patrick.Arbuthnot@wits.ac.za.
authors.forename: Patrick
authors.initials: P
authors.lastname: Arbuthnot
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D016503:Drug Delivery Systems / Q000009:adverse effects / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism*; D023281:Genomics; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000502:physiology; D022781:Hepatocytes / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000502:physiology / Q000821:virology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D016207:Cytokines; D006514:Hepatitis B Surface Antigens; D035683:MicroRNAs; D001616:beta-Galactosidase
keywords: Adenoviral vectors; Immunocytochemistry; Northern blot; Q-PCR; RNA interference; X-gal staining; microRNA