title: Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease.
abstract: An abasic site modified fluorescent probe has been developed which enabled the rapid discrimination of stable single mismatched base pairs by lambda exonuclease with remarkably high discrimination factors (447 for T:G and 238 for A:G). This method allowed sensitive detection of single nucleotide variation at very low abundances (0.02-0.05% mutant-to-wild type).
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.
authors.forename: Tongbo
authors.initials: T
authors.lastname: Wu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.
authors.forename: Feidan
authors.initials: F
authors.lastname: Gu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry; D053614:Janus Kinase 2 / Q000235:genetics; D015336:Molecular Probe Techniques; D017354:Point Mutation; D051981:Uracil-DNA Glycosidase / Q000235:genetics
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D005663:Furans; D053614:Janus Kinase 2; D005090:Exodeoxyribonucleases; D051981:Uracil-DNA Glycosidase