title: Complete chloroplast genomes of two Miscanthus species.
abstract: The complete chloroplast (cp) genomes of two Miscanthus species, M. sinensis and M. sacchariflorus, were sequenced and investigated for genes, genome size variation, and polymorphisms. There are 170 genes in both cp genomes, consisting of 122 mRNA genes (84 protein-coding genes and 38 hypothetical genes), 40 tRNA genes, and 8 rRNA genes. The cp genome contains two inverted repeat (IR) regions, separated by large single copy (LSC) region and small single copy (SSC) region. Indels were responsible for 40 bp difference in cp genome size in two species. In addition, we established phylogenetic relationship with other monocot cp genomes, and estimated divergence time. The two Miscanthus species clustered together among other C4monocot species and the divergence time of two Miscanthus species was approximately 0.5 1-0.84 Mya.
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Gyoungju
authors.initials: G
authors.lastname: Nah
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Im
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Lim
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Kyunghee
authors.initials: K
authors.lastname: Kim
authors.affiliation: b College of Agriculture and Life Sciences, National Instrumentation Center for Environment Management (NICEM), Seoul National University , Seoul , Korea , and.
authors.forename: Ah Young
authors.initials: AY
authors.lastname: Choi
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Yook
authors.affiliation: c Biological and Genetic Resources Assessment Division, National Institute of Biological Resources , Incheon , Korea.
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.affiliation: c Biological and Genetic Resources Assessment Division, National Institute of Biological Resources , Incheon , Korea.
authors.forename: Changmu
authors.initials: C
authors.lastname: Kim
authors.affiliation: a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
authors.forename: Do-Soon
authors.initials: DS
authors.lastname: Kim
mesh_terms: D001482:Base Composition / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D005075:Biological Evolution; D002736:Chloroplasts / Q000235:genetics; D018742:DNA, Chloroplast / Q000235:genetics; D023061:Gene Order; D050259:Genes, Mitochondrial / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D016678:Genome / Q000235:genetics; D054628:Genome, Chloroplast / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D008928:Mitochondria / Q000235:genetics; D010802:Phylogeny; D006109:Poaceae / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018742:DNA, Chloroplast
keywords: Chloroplast genome; divergence time, Miscanthus; next-generation sequencing; phylogeny