title: Aluminum trichloride impairs bone and downregulates Wnt/β-catenin signaling pathway in young growing rats.
abstract: Aluminum (Al) can accumulate in bone and cause bone diseases. Few studies have investigated molecular mechanism of Al-induced bone diseases. Thus, in this study, rats were orally exposed to 0 (control group) and 0.4 g/L aluminum trichloride (AlCl3) (treatment group) for 30, 60, 90 or 120 days, respectively. The Al content of femora and serum, bone histological structure, bone mineral density (BMD) of the distal and proximal femoral metaphysis and Wnt/β-catenin signaling pathway (the mRNA expressions of Wnt3a, Fzd2, LRP-5, β-catenin, Tcf4, cyclin D1 and c-Myc, the protein levels of Wnt3a and β-catenin, the activities of Fzd2 and LRP-5) in rat femora were determined on day 30, 60, 90 or 120, respectively. The results showed that the Al contents of femora and serum were increased, the BMD of the distal and proximal femoral metaphysis were decreased, the femora histological structure were disrupted, the mRNA expressions of Wnt3a, Fzd2, LRP-5, β-catenin, Tcf4, cyclin D1 and c-Myc, the protein levels of Wnt3a and β-catenin, the activities of Fzd2 and LRP-5 were all decreased in the treatment group compared with the control group with time prolonged. These results indicated that AlCl3 impaired femora by inhibiting the Wnt/β-catenin signaling pathway in young growing rats.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Animal Biology, The University of Western Australia, 35 Stirling Highway Crawley, WA 6009, Australia.
authors.forename: Shimin
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Feibo
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Che
authors.affiliation: Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China.
authors.forename: Yanzhu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China. Electronic address: liyanfei@neau.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Harbin Pharmaceutical Group Bio-vaccine Limited Liability Company, Harbin 150069, China.
authors.forename: Chuanyi
authors.initials: C
authors.lastname: Pan
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Harbin Pharmaceutical Group Bio-vaccine Limited Liability Company, Harbin 150069, China.
authors.forename: Wannan
authors.initials: W
authors.lastname: Liang
mesh_terms: D000535:Aluminum / Q000097:blood; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002712:Chlorides / Q000378:metabolism / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D017607:Aluminum Compounds; D002712:Chlorides; D012333:RNA, Messenger; D051153:Wnt Proteins; D051176:beta Catenin; D000077410:Aluminum Chloride; D000535:Aluminum
keywords: Aluminum trichloride; Femora; Rat; Wnt/β-catenin signaling pathway