title: Evaluation of Chicken IgY Generated Against Canine Parvovirus Viral-Like Particles and Development of Enzyme-Linked Immunosorbent Assay and Immunochromatographic Assay for Canine Parvovirus Detection.
abstract: Immunoglobulin Y (IgY) antibodies were generated against canine parvovirus virus-like particles (CPV-VLPs) antigen using chickens. Anti-CPV-VLPs-IgY was extracted from hen egg yolk and used for developing enzyme-linked immunosorbent assay (ELISA) and immunochromatographic assay (ICA) for the detection of CPV in dog feces. The cutoff negative values for anti-CPV-VLPs-IgY were determined using negative fecal samples (already confirmed by polymerase chain reaction [PCR]). In both ELISA and ICA, there was no cross-reaction with other diarrheal pathogens. Thirty-four fecal samples were collected from dogs with diarrhea, of which 26.47% were confirmed as CPV-positive samples by PCR, while 29.41% and 32.35% of the samples were found to be positive by ELISA and ICA, respectively. The developed ELISA and ICA exhibited 97.06% and 94.12% conformity with PCR. Higher sensitivity and specificity were observed for IgY-based ELISA and ICA. Thus, they could be suitable for routine use in the diagnosis of CPV in dogs.
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, People's Republic of China .
authors.forename: Jinxin
authors.initials: J
authors.lastname: He
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, People's Republic of China .
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: 2 Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, China .
authors.forename: Shiqi
authors.initials: S
authors.lastname: Sun
authors.affiliation: 1 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, People's Republic of China .
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D007136:Immunoglobulins / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins; D058425:Vaccines, Virus-Like Particle