title: Promotion of acute-phase skin wound healing by Pseudomonas aeruginosa C4-HSL.
abstract: A Pseudomonas aeruginosa quorum-sensing system, which produces N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL) and N-butanoyl-l-homoserine lactone (C4-HSL), regulates the virulence factors. In our previous study, 3-oxo-C12-HSL, encoded by lasI gene, was shown to promote wound healing. However, the effect of C4-HSL, encoded by rhlI gene, remains to be elucidated. We addressed the effect of C4-HSL on wounds in P. aeruginosa infection. Wounds were created on the backs of Sprague-Dawley SD rats, and P. aeruginosa PAO1 (PAO1) or its rhlI deletion mutant (ΔrhlI) or lasI deletion mutant (ΔlasI) was inoculated onto the wound. Rats were injected intraperitoneally with anti-C4-HSL antiserum or treated with C4-HSL at the wound surface. PAO1 inoculation led to significant acceleration of wound healing, which was associated with neutrophil infiltration and TNF-α synthesis. These responses were reversed, except for TNF-α production, when ΔrhlI was inoculated instead of PAO1 or when rats were co-treated with PAO1 and anti-C4-HSL antiserum. In contrast, the healing process and neutrophil infiltration, but not TNF-α synthesis, were accelerated when C4-HSL was administered in the absence of PAO1. This acceleration was not affected by anti-TNF-α antibody. These results suggest that C4-HSL may be involved in the acceleration of acute wound healing in P. aeruginosa infection by modifying the neutrophilic inflammation.
authors.affiliation: Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Kanno
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawakami
authors.affiliation: Laboratory of Organic Chemistry, School of Pharmacy, Nihon University, Chiba, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Miyairi
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Tanno
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Kamimatsuno
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takagi
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Miyasaka
authors.affiliation: Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Microbiology and Infection Control Sciences, Kyoto Pharmaceutical University, Kyoto, Japan.
authors.forename: Naomasa
authors.initials: N
authors.lastname: Gotoh
authors.affiliation: Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Maruyama
authors.affiliation: Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tachi
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D001707:Biopsy, Needle; D004195:Disease Models, Animal; D005260:Female; D006714:Homoserine / Q000031:analogs & derivatives / Q000494:pharmacology; D007150:Immunohistochemistry; D009504:Neutrophils / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*; D014947:Wounds and Injuries / Q000473:pathology* / Q000628:therapy*
chemical_list: C109860:N-(3-oxododecanoyl)homoserine lactone; C092312:N-butyrylhomoserine lactone; D014409:Tumor Necrosis Factor-alpha; C088386:homoserine lactone; D006714:Homoserine; D015107:4-Butyrolactone
keywords: N-butanoyl-l-homoserine lactone; Pseudomonas aeruginosa; Quorum sensing; Wound healing