title: Are there any effects of chronic carbon monoxide exposure on argyrophilic nucleolar-organizing region-associated protein synthesis in rat myocardium?
abstract: The aims of the study are to detect whether there are any possible effects of chronic carbon monoxide (CO) exposure on the argyrophilic nucleolar-organizing region (AgNOR)-associated protein synthesis and evaluate any possible relationship between the amount of AgNOR protein and the level of myocardial injury also and between AgNOR and histopathological evaluation methods. Adult male albino Wistar rats (n = 18) were randomly divided into three groups (groups A, B, and C). Group A served as control, while groups B and C were rats exposed to CO gas chronically (1000 and 3000 ppm CO concentration with a flow rate of 4 L/min for 30 min/day for 7 days, respectively). Total AgNOR area/nuclear area (TAA/NA) and the mean AgNOR numbers for each myocyte nucleus were determined. There were significant differences among all groups for TAA/NA ratio. These differences were not significant for mean AgNOR numbers. According to the histopathological evaluation scores, there were significant differences between the groups. The differences were significant among the groups for loss of sarcomere pattern. A strong positive correlation between histopathological injury scores and TAA/NA ratio was found (Rsq = 0.48; p = 0.002), however, the correlation was not significant for mean AgNOR numbers (Rsq = 0.08; p = 0.25). In conclusion, TAA/NA ratio can be used as an indicator for obtaining information about the level of myocardial damage instead of histopathological evaluation scores.
authors.affiliation: Department of Emergency Medicine, Duzce University School of Medicine, Duzce, Turkey a_saritas_@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Saritas
authors.affiliation: Department of Emergency Medicine, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Gunes
authors.affiliation: Department of Anatomy, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Colakoglu
authors.affiliation: Department of Medical Genetics, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: R
authors.initials: R
authors.lastname: Eroz
authors.affiliation: Department of Emergency Medicine, Ataturk University School of Medicine, Erzurum, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Akoz
authors.affiliation: Department of Pathology, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Oktay
authors.affiliation: Department of Radiology, Duzce State Hospital, Duzce, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Buyukkaya
authors.affiliation: Department of Emergency Medicine, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Kandis
authors.affiliation: Department of Pediatric Surgery, Duzce University School of Medicine, Duzce, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Ozkan
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear / Q000096:biosynthesis*; D015415:Biomarkers / Q000378:metabolism; D002248:Carbon Monoxide / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D019570:Inhalation Exposure / Q000009:adverse effects*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D009697:Nucleolus Organizer Region / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D012518:Sarcomeres / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D034961:Antigens, Nuclear; D015415:Biomarkers; C051963:nucleolar organizer region associated proteins; D002248:Carbon Monoxide
keywords: AgNOR; Chronic carbon monoxide intoxication; myocardium