title: Molecular identification and phylogenetic analysis of Metarhabditis blumi (Nematoda: Rhabditida).
abstract: The species Metarhabditis blumi was diagnosed for the first time in Brazil using a molecular assay. Parasites isolated from two cattle herds from the state of Minas Gerais, southeastern Brazil, were classified as M. blumi according to their D2/D3 expansion fragments of the 28S rDNA sequence, which had a high degree of homology (100% similarity) with a M. blumi sequence deposited in GenBank (EU195965.1). The phylogenetic tree comparison also confirmed grouping of the populations evaluated in the current study as M. blumi.
authors.affiliation: Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil. Electronic address: patriciavbossi@gmail.com.
authors.forename: Patrícia Vieira
authors.initials: PV
authors.lastname: Bossi
authors.affiliation: Biological Institute, Campinas, São Paulo, Brazil.
authors.forename: Erika Aparecida
authors.initials: EA
authors.lastname: Consoli
authors.affiliation: Biological Institute, Campinas, São Paulo, Brazil.
authors.forename: Juliana Magrinelli Osório
authors.initials: JM
authors.lastname: Rosa
authors.affiliation: Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Luiza Bossi
authors.initials: LB
authors.lastname: Leite
authors.affiliation: Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Romário Cerqueira
authors.initials: RC
authors.lastname: Leite
authors.affiliation: Biological Institute, Campinas, São Paulo, Brazil.
authors.forename: Claudio Marcelo Gonçalves
authors.initials: CM
authors.lastname: de Oliveira
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D010802:Phylogeny; D018510:RNA, Helminth / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D017168:Rhabditida / Q000235:genetics*; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D018510:RNA, Helminth; D012339:RNA, Ribosomal, 28S
keywords: 28S rDNA; Cattle; Nematodes; Parasitic otitis