title: MicroRNA-126 regulates migration and invasion of gastric cancer by targeting CADM1.
abstract: OBJECTIVE
  The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of gastric cancer (GC). We here aimed to investigate the mechanism of microRNAs in the regulation of GC pathogenesis.
  
  
  METHODS
  Transwell chambers (8-μM pore size; Costar) were used in the in vitro migration and in vision assay. Dual luciferase reporter gene construct and dual luciferase reporter assay to identify the target of miR-126. CADM1 expression was evaluated by immunohistochemical staining. The clinical manifestations, treatments and survival were collected for statistical analysis.
  
  
  RESULTS
  Inhibition of miR-126 effectively reduced migration and invasion of gastric cancer cell lines. Bioinformatics and luciferase reporter assay revealed that miR-126 specifically targeted the 3'UTR of cell adhesion molecule 1 (CADM1) and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of GC cell lines. Furthermore, in tumor tissues obtained from gastric cancer patients, the expression of miR-126 was negatively correlated with CADM1 and the high expression of miR-126 combined with low expression of CADM1 might serve as a risk factor for stage1 gastric cancer patients.
  
  
  CONCLUSIONS
  Our study showed that miR-126, by down-regulation CADM1, enhances migration and invasion in GC cells.
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Zhengzhou University China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of General Surgery, First Renmin Hospital of Shangqiu Henan Province, China.
authors.forename: Ruoming
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Cancer, First Renmin Hospital of Shangqiu Henan Province, China.
authors.forename: Tengteng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Surgery, First Affiliated Hospital of Zhengzhou University China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Dong
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C527111:MIRN126 microRNA, human; D035683:MicroRNAs
keywords: Gastric cancer (GC); cell adhesion molecule 1 (CADM1); invasion; microRNA-126