title: Multiplex-PCR for Identification of Two Species in Genus Hishimonus (Hemiptera: Cicadellidae) in Jujube Orchards.
abstract: The insect family Cicadellidae includes economically important vectors of plant pathogens. Hishimonus sellatus (Uhler) transmits jujube witches'-broom (JWB). Currently, H. sellatus and Hishimonus lamellatus Cai et Kuoh are observed to co-occur at the same locality on jujube. H. lamellatus is now suspected to be a JWB vector. As such, correct identification of Hishimonus species present in vineyards is essential for epidemiological surveys. However, traditional identification of Hishimonus by morphology is limited to the adult male. We provide a comprehensive description of morphological and molecular tools for discriminating between H. sellatus and H. lamellatus, for use in identification and monitoring of the two Hishimonus species and studies of their plant hosts. A rapid and inexpensive method is introduced to identify H. sellatus and H. lamellatus occurring in jujube orchards. This method is based on amplification of mitochondrial cytochrome oxidase I (COI) gene, using PCR with multiplexed, species-specific primers. The reliability of this new method has been tested on different populations from different sites in Beijing region of China.
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Shaodong
authors.initials: S
authors.lastname: Hao
authors.affiliation: Beijing Forest Protection Station,Beijing 100029, China.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: Beijing Forest Protection Station,Beijing 100029, China.
authors.forename: Wanqiang
authors.initials: W
authors.lastname: Tao
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China. jinzhw9276@163.com.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Pinggu Branch School of Beijing Agricultural Broadcast Television College, Beijing 101200, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Minzhao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Li
authors.initials: L
authors.lastname: Guo
authors.affiliation: Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Shi
mesh_terms: D000818:Animals; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D006430:Hemiptera / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D031957:Ziziphus / Q000254:growth & development
chemical_list: D019476:Insect Proteins; D024101:Mitochondrial Proteins; D003576:Electron Transport Complex IV
keywords: Hishimonus lamellatus; Hishimonus sellatus; multiplex-PCR; jujube