title: Yeasts from native Brazilian Cerrado plants: Occurrence, diversity and use in the biocontrol of citrus green mould.
abstract: Yeasts are some of the most important postharvest biocontrol agents. Postharvest oranges frequently deteriorate due to green mould (Penicillium digitatum), which causes significant losses. The aims of this study were to determine the composition and diversity of yeasts on plants of the Brazilian Cerrado and to explore their potential for inhibiting citrus green mould. Leaves and fruit of Byrsonima crassifolia and Eugenia dysenterica were collected from Cerrado conservation areas, and thirty-five yeasts were isolated and identified by sequencing the D1-D2 domain of the rDNA large subunit (26S). The isolates represented the Aureobasidium, Meyerozyma, Candida, and Pichia genera. Three isolates identified as Aureobasidium pullulans exhibited potential for the control of P. digitatum in both in vitro and in vivo tests; these isolates reduced the incidence of disease and increased the storage time of fruit. Aureobasidium. pullulans has immense potential for the biological control of filamentous fungi.
authors.affiliation: Departamento de Fitopatologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, 70910-900, Brazil. Electronic address: eugeniosperandio@gmail.com.
authors.forename: Eugenio Miranda
authors.initials: EM
authors.lastname: Sperandio
authors.affiliation: Departamento de Fitopatologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, 70910-900, Brazil. Electronic address: helson@unb.br.
authors.forename: Helson Mario
authors.initials: HM
authors.lastname: Martins do Vale
authors.affiliation: Departamento de Fitopatologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, 70910-900, Brazil. Electronic address: gamm.bio@gmail.com.
authors.forename: Geisianny Augusta Monteiro
authors.initials: GAM
authors.lastname: Moreira
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D001938:Brazil; D002957:Citrus / Q000382:microbiology*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D000068856:Eugenia / Q000382:microbiology*; D006801:Humans; D029581:Malpighiaceae / Q000382:microbiology*; D056265:Microbial Interactions; D008969:Molecular Sequence Data; D010407:Penicillium / Q000254:growth & development*; D010572:Pest Control, Biological / Q000379:methods; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D018515:Plant Leaves / Q000382:microbiology; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
keywords: Aureobasidium pullulans; Penicillium digitatum; Postharvest diseases; Yeast diversity