title: Heat shock proteins and proteasomal degradation in normal and tumor cells.
abstract: Proteasomal degradation of oxidized proteins is a crucial mechanism to prevent the accumulation of cellular damage. The removal of the damage is generally a required process for healthy organisms to keep the integrity while in cancer cells the situation may be different. In normal conditions, cancer cells have higher proteasome activity compared to normal cells. During cancer treatment, cellular damage by chemotherapy is an expected process to be able to kill the tumor cells. And the accumulation of this damage accompanied by the decrease in protein repair and removal systems may increase the efficacy of the cancer therapy. Heat shock proteins (Hsp) as molecular chaperones are involved in the folding, activation and assembly of a variety of proteins. Among these Hsp40, Hsp70 and Hsp90 are believed to act as a chaperone system to regulate the proteasomal degradation. In this study, we tested the role of heat stress response on the proteasomal degradation of oxidized proteins. We used two different cell lines to observe the difference in normal and tumor cells. First the effect of heat stress (42°C, 1h) were tested in terms of protein oxidation tested by protein carbonyl formation and proteasomal degradation. The results were extremely different in normal fibroblast cells and hippocampal tumor cells. In the same direction, the expressions of Hsp40, Hsp70 and Hsp90 were affected in a different manner in two cell lines, will be discussed in detail. Supported by TUBITAK COST-CM1001-110S281.
authors.affiliation: Marmara University (Genetic and Metabolic Diseases Research and Investigation Center), Department of Biochemistry, Turkey. Electronic address: betulkarademir@marmara.edu.tr.
authors.forename: Betul
authors.initials: B
authors.lastname: Karademir
authors.affiliation: Marmara University (Genetic and Metabolic Diseases Research and Investigation Center), Department of Biochemistry, Turkey.
authors.forename: Perinur
authors.initials: P
authors.lastname: Bozaykut
authors.affiliation: Marmara University (Genetic and Metabolic Diseases Research and Investigation Center), Department of Biochemistry, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Kartal Ozer