title: Flavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier.
abstract: A novel rod-shaped, Gram-stain-negative, non-gliding and aerobic strain surrounded by a multilayer capsule, designated 4-T-2T, was isolated from a till sample of Collins glacier front, Antarctica. The bacterium formed yellow, circular, convex and smooth colonies. Growth occurred at 4-28 °C (optimum18-20 °C), at pH 7.0-10.0 (optimum pH 9.0) and with 0-1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4-T-2T belonged to the genus Flavobacterium. Strain 4-T-2T shared the highest 16S rRNA gene sequence similarity with the type strain of Flavobacterium algicola (96.7 %). The DNA G+C content of strain 4-T-2T was 36.2 mol%. The only menaquinone was MK-6. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C16 : 0 and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0). Polar lipid profile consisted phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and one unidentified lipid. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 4-T-2T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium collinsense sp. nov. is proposed. The type strain is 4-T-2T ( = CCTCC AB 2014004T = LMG 28257T).
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xulu
authors.initials: X
authors.lastname: Chang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Qiu
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Lvzhi
authors.initials: L
authors.lastname: Ren
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Qu
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Sangsang
authors.initials: S
authors.lastname: Deng
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Xuyang
authors.initials: X
authors.lastname: Da
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Chengxiang
authors.initials: C
authors.lastname: Fang
authors.affiliation: China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Peng
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6