title: Impact of starch content on protein adsorption characteristics in amphiphilic hybrid graft copolymers.
abstract: Amphiphilic hybrid graft copolymers were synthesized using a graft-to methodology and their protein adsorption profiles studied. Three different hydrophilic side chains were studied: hydroxypropylated high amylose starch, maltodextrin, and polyethylene glycol (PEG). In the high amylose starch compositions, there was a pronounced decrease in protein adsorption with increasing polysaccharide content. As the starch content in the graft copolymers increased from 10 wt% to 53 wt%, BSA protein adsorption decreased by 83% whereas fibrinogen adsorption was reduced by 40%. Comparisons between the starch-containing hybrid polymers and their respective hydrophobic urethane-linked polyesters were also made. Hybrid 53, containing 53 wt% starch, showed a 85% reduction in BSA adsorption and 51% reduction in fibrinogen relative to their urethane-linked polyester backbone controls. Grafting branched high amylopectin-derived maltodextrin to the synthetic polymer backbones also conferred modest protein resistance to the hydrophobic backbone polymer. Lastly, it was found that a high amylose graft structure provided comparable, if not slightly more effective, protein resistance compared to a similarly constructed PEG-containing amphiphilic copolymer.
authors.affiliation: Department of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, United States.
authors.forename: Arijit
authors.initials: A
authors.lastname: Sengupta
authors.affiliation: Department of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, United States.
authors.forename: Allison R
authors.initials: AR
authors.lastname: Linehan
authors.affiliation: Department of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, United States. Electronic address: piovine@sandiego.edu.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Iovine
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D011092:Polyethylene Glycols; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols; D013213:Starch
keywords: Graft copolymer; Protein adsorption; Starch