title: Quantitative Proteomics Analysis of Herbaceous Peony in Response to Paclobutrazol Inhibition of Lateral Branching.
abstract: Herbaceous peony (Paeonia lactiflora Pall.) is an emerging high-grade cut flower worldwide, which is usually used in wedding bouquets and known as the "wedding flower". However, abundant lateral branches appear frequently in some excellent cultivars, and a lack of a method to remove Paeonia lactiflora lateral branches other than inefficient artificial methods is an obstacle for improving the quality of its cut flowers. In this study, paclobutrazol (PBZ) application was found to inhibit the growth of lateral branches in Paeonia lactiflora for the first time, including 96.82% decreased lateral bud number per branch, 77.79% and 42.31% decreased length and diameter of lateral branches, respectively, declined cell wall materials and changed microstructures. Subsequently, isobaric tag for relative and absolute quantitation (iTRAQ) technology was used for quantitative proteomics analysis of lateral branches under PBZ application and control. The results indicated that 178 differentially expressed proteins (DEPs) successfully obtained, 98 DEPs were up-regulated and 80 DEPs were down-regulated. Thereafter, 34 candidate DEPs associated with the inhibited growth of lateral branches were screened according to their function and classification. These PBZ-stress responsive candidate DEPs were involved in eight biological processes, which played a very important role in the growth and development of lateral branches together with the response to PBZ stress. These results provide a better understanding of the molecular theoretical basis for removing Paeonia lactiflora lateral branches using PBZ application.
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. dqzhao@yzu.edu.cn.
authors.forename: Daqiu
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. gong.sai.jie@163.com.
authors.forename: Saijie
authors.initials: S
authors.lastname: Gong
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. haozhaojunko@126.com.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. jsmeng@yzu.edu.cn.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Meng
authors.affiliation: Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. taojun@yzu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Tao
mesh_terms: D001692:Biological Transport / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology; D035264:Flowers / Q000254:growth & development*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D029596:Paeonia / Q000254:growth & development*; D040901:Proteomics; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D007210:Indoleacetic Acids; D014230:Triazoles; C053370:paclobutrazol; C030737:indoleacetic acid
keywords: herbaceous peony; iTRAQ; paclobutrazol application; proteomics analysis