title: A Novel Point-of-Care BioNanoSensor for Rapid HIV Detection and Treatment Monitoring.
abstract: We report here a new diagnostic approach to the direct detection of HIV in blood or other body fluids that is rapid, sensitive and potentially applicable in a point-of-care setting. The approach follows on the development of a novel BioNanoSensor (BNS) device that utilizes piezoelectric technology to detect the presence of the HIV surface glycoprotein gp120 in a nanoscale format. The detection range of the BNS device for the biomarker gp120 displayed a low-end sensitivity of 6.5×104HIV viral particles/ml, while using a small fluid sample (5 µl) and with a reaction time of less then 30 seconds. Performance of this device indicated that the BNS has utility for direct detection of HIV particles prior to, and independent from, antibody formation. Accordingly, this device holds utility to monitor the status of HIV infection both early after exposure to virus as well as during chronic HIV infection. The BNS parameters of small sample volume, compact device size, and detection sensitivity indicate that the BNS is potentially useful in the point-of-care and/or home setting for monitoring decisions regarding HIV treatment on a real-time basis.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, USA.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rozmyslowicz
authors.affiliation: School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, USA.
authors.forename: Johann
authors.initials: J
authors.lastname: deSa
authors.affiliation: School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, USA.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lec
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, USA.
authors.forename: Glen N
authors.initials: GN
authors.lastname: Gaulton
keywords: BioNanoSensors; Electronic Measurement System; HIV detection; HIV treatment monitoring; HIV-1 infection; Piezoelectric high frequency technology; Polyclonal sheep anti-HIV-1 gp120