title: SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.
abstract: SiRNA therapeutics promise a future where any target in the transcriptome could be potentially addressed. However, the delivery of SiRNAs and targeting of particular cell types or organs are major challenges. A novel, efficient, and safe delivery system for promising the introduction of SiRNAs into particular cell types within living organisms is of great significance. Our previous studies have proved that recombinant protein (MSTN) and exogenous gene (EGFP) as vaccines, and furthermore functional CD40 shRNA expression can be delivered into dendritic cells (DCs) in mouse by oral administration of recombinant yeast (Saccharomyces cerevisiae). Here, we describe the details of the promising and innovative approach based on oral administration of recombinant yeast that allows in vivo-targeted delivery of functional SiRNA to murine intestinal DCs.
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Zhongtian
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.|Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi, 030006, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China. zhangzhy@nwsuaf.edu.cn.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002469:Cell Separation; D004274:DNA, Recombinant / Q000235:genetics*; D003713:Dendritic Cells / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D007422:Intestines / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014162:Transfection
chemical_list: D004274:DNA, Recombinant; D034741:RNA, Small Interfering
keywords: Dendritic cell s; Oral administration; S. cerevisiae; SiRNA; Targeted delivery; Yeast