title: An optimised perfusion technique for extracting murine gastric leukocytes.
abstract: The stomach is a difficult tissue to analyse by flow cytometry, largely due to the difficulty of isolating viable leukocytes. Here we present the re-optimization of a perfusion technique that compares favourably against two other methods of enzymatic digestion for the release of gastric leukocytes. We believe that this technique could greatly assist the analysis of immune cells basally present in the murine stomach and that infiltrate during infection or disease.
authors.affiliation: Mucosal Immunology, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia. Electronic address: garrett.ng@mcri.edu.au.
authors.forename: Garrett Z
authors.initials: GZ
authors.lastname: Ng
authors.affiliation: Mucosal Immunology, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Centre for Animal Biotechnology, School of Veterinary and Agricultural Science, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Sutton
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010477:Perfusion / Q000379:methods*; D013270:Stomach / Q000166:cytology* / Q000276:immunology*
keywords: Flow cytometry; Leukocytes; Stomach