title: Resonance light scattering determination of 6-mercaptopurine coupled with HPLC technique.
abstract: A simple, fast, costless, sensitive and selective method of resonance light scattering coupled with HPLC was established for the determination of 6-mercaptopurine in human urine sample. In a Britton-Robinson buffer solution of pH5.5, the formation of coordination complex between 6-mercaptopurine and metal palladium (II) led to enhance the RLS intensity of the system. The RLS signal was detected by fluorescence detector at λ(ex)=λ(em)=315 nm. The analytical parameters were provided by the coupled system, the linear of 6-mercaptopurine response from 0.0615 to 2.40 μg L(-1) and the limit of detection (S/N=3) was 0.05 μg L(-1). The presented method has been applied to determine 6-mercaptopurine in human urine samples which obtained satisfactory results. Moreover, the reaction mechanism and possible reasons for enhancement of RLS were fully discussed.
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, Beibei District, Chongqing, PR China.
authors.forename: Ai Ping
authors.initials: AP
authors.lastname: Li
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, Beibei District, Chongqing, PR China. Electronic address: hxpengjd@swu.edu.cn.
authors.forename: Jing Dong
authors.initials: JD
authors.lastname: Peng
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, Beibei District, Chongqing, PR China.
authors.forename: MingQiong
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Chemistry and Chemical Engineering, Southwest University, Beibei District, Chongqing, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D000067493:Dynamic Light Scattering / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D015122:Mercaptopurine / Q000652:urine*; D008958:Models, Molecular
chemical_list: D000964:Antimetabolites, Antineoplastic; D015122:Mercaptopurine
keywords: 6-mercaptopurine; HPLC; Resonance light scattering