title: Ebolavirus Glycoprotein Directs Fusion through NPC1+ Endolysosomes.
abstract: Ebolavirus, a deadly hemorrhagic fever virus, was thought to enter cells through endolysosomes harboring its glycoprotein receptor, Niemann-Pick C1. However, an alternate model was recently proposed in which ebolavirus enters through a later NPC1-negative endosome that contains two-pore Ca(2+) channel 2 (TPC2), a newly identified ebolavirus entry factor. Here, using live cell imaging, we obtained evidence that in contrast to the new model, ebolavirus enters cells through endolysosomes that contain both NPC1 and TPC2.
authors.affiliation: Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Simmons
authors.affiliation: Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Ryan S
authors.initials: RS
authors.lastname: D'Souza
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
authors.forename: Margarida
authors.initials: M
authors.lastname: Ruas
authors.affiliation: Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
authors.forename: Antony
authors.initials: A
authors.lastname: Galione
authors.affiliation: Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Casanova
authors.affiliation: Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA jw7g@virginia.edu.
authors.forename: Judith M
authors.initials: JM
authors.lastname: White
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D029043:Ebolavirus / Q000502:physiology*; D011992:Endosomes / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008853:Microscopy; D008954:Models, Biological; D000077296:Niemann-Pick C1 Protein; D014759:Viral Envelope Proteins / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D015220:Calcium Channels; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; C106881:NPC1 protein, human; D000077296:Niemann-Pick C1 Protein; C530224:TPCN2 protein, human; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus