title: [Measurement of S-adenosylmethionine and S-adenosylhomocysteine in mice liver with high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a method for determination of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) simultaneously in mice liver by reversed-phase high performance liquid chromatography (HPLC).
  
  
  METHODS
  Mice livers were weighed, emulsified quickly by handheld homogenate, four times the volume of 0.6 mol/L HCLO4 added for ultrasonic, and the hydrolysate was adjusted to centrifuged and filtrated with a membrane. The supernatants were separatedonVenusil MP-C18 column (250 mm x 4.6 mm, 5 µm) at 30 °C with a mobile phase of 50 mmol/L NaH2PO4, 10 mmol/L C7H15NaO3S and methanol, a flow rate of 1.0 ml/min and UV detection at 254 nm.
  
  
  RESULTS
  The SAM and SAH in the corresponding concentration range showed a good linear relation with its peak area, correlation coefficient ( r > 0. 9990 ) , recovery was 92.20%-101.38%, RSD was 2.88%-6.78%. The average within-day precision of SAM and SAH was 4.14% and 3.71%, and the average day to day precision was 7.51% and 9.54%. The content of SAM and SAH in mice liver was 3.14-6.09 mg/L (31.44-60.98 nmol/g wet weight) and 1.29-3.10 mg/L (13.38-32.17 nmol/g wet weight) respectively.
  
  
  CONCLUSIONS
  The validated method is simple, rapid accurate and reliable to the determination of SAM and SAH in mice liver.
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Wu
authors.forename: Shijing
authors.initials: S
authors.lastname: Zhao
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: Jian'gang
authors.initials: J
authors.lastname: Zhao
authors.forename: Guowei
authors.initials: G
authors.lastname: Huang
mesh_terms: D000818:Animals; D001835:Body Weight; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005374:Filtration; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry*; D051379:Mice; D012435:S-Adenosylhomocysteine / Q000097:blood*; D012436:S-Adenosylmethionine / Q000097:blood*; D014465:Ultrasonics
chemical_list: D007202:Indicators and Reagents; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine