title: Poly(3-Hydroxybutyrate) Production in Repeated fed-Batch with Cell Recycle Using a Medium with low Carbon Source Concentration.
abstract: Among approaches applied to obtain high productivity and low production costs in bioprocesses are high cell density and the use of low cost substrates. Usually low cost substrates, as waste/agroindustrial residues, have low carbon concentration, which leads to a difficulty in operating bioprocesses. Real time control of process for intracellular products is also difficult. The present study proposes a strategy of repeated fed-batch with cell recycle to attain high cell density of Cupriavidus necator and high poly(3-hydroxybutyrate) (P(3HB)) productivity, using a substrate with low carbon source concentration (90 g l(-1)). Also, the use of the oxygen uptake rate data was pointed out as an on line solution for process control, once P(3HB) is an intracellular product. The results showed that total biomass (X), residual biomass (Xr) and P(3HB) values at the end of the culture were 61.6 g l(-1), 19.3 g l(-1) and 42.4 g l(-1) respectively, equivalent to 68.8 % of P(3HB) in the cells, and P(3HB) productivity of 1.0 g l(-1) h(-1). Therefore, the strategy proposed was efficient to achieve high productivity and high polymer content from a medium with low carbon source concentration.
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), PO box 6192, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: Jaciane Lutz
authors.initials: JL
authors.lastname: Ienczak
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
authors.forename: Mélodi
authors.initials: M
authors.lastname: Schmidt
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
authors.forename: Luci Kelin
authors.initials: LK
authors.lastname: Quines
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
authors.forename: Kellen
authors.initials: K
authors.lastname: Zanfonato
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), PO box 6192, Campinas, SP, CEP 13083-970, Brazil.
authors.forename: José Geraldo
authors.initials: JG
authors.lastname: da Cruz Pradella
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
authors.forename: Willibaldo
authors.initials: W
authors.lastname: Schmidell
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil. gmf.aragao@gmail.com.
authors.forename: Glaucia Maria Falcao
authors.initials: GM
authors.lastname: de Aragao
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D020118:Cupriavidus necator / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D007700:Kinetics; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Cell recycle; Cross flow filtration; Low carbon source concentration; Poly(3-hydroxybutyrate); Repeated fed-batch.