title: Green synthesis and characterization of silver nanoparticles using Artemisia absinthium aqueous extract--A comprehensive study.
abstract: Unlike chemical synthesis, biological synthesis of nanoparticles is gaining tremendous interest, and plant extracts are preferred over other biological sources due to their ample availability and wide array of reducing metabolites. In this project, we investigated the reducing potential of aqueous extract of Artemisia absinthium L. for synthesizing silver nanoparticles (AgNPs). Optimal synthesis of AgNPs with desirable physical and biological properties was investigated using ultra violet-visible spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM) and energy-dispersive X-ray analysis (EDX). To determine their appropriate concentrations for AgNP synthesis, two-fold dilutions of silver nitrate (20 to 0.62 mM) and aqueous plant extract (100 to 0.79 mg ml(-1)) were reacted. The results showed that silver nitrate (2mM) and plant extract (10 mg ml(-1)) mixed in different ratios significantly affected size, stability and yield of AgNPs. Extract to AgNO3 ratio of 6:4v/v resulted in the highest conversion efficiency of AgNO3 to AgNPs, with the particles in average size range of less than 100 nm. Furthermore, the direct imaging of synthesized AgNPs by TEM revealed polydispersed particles in the size range of 5 to 20 nm. Similarly, nanoparticles with the characteristic peak of silver were observed with EDX. This study presents a comprehensive investigation of the differential behavior of plant extract and AgNO3 to synthesize biologically stable AgNPs.
authors.affiliation: Mid-Florida Research and Education Center and Department of Plant Pathology, University of Florida/Institute of Food and Agricultural Sciences, 2725 Binion Rd., Apopka, FL 32703, United States.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ali
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL 32816, United States.
authors.forename: Bosung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemistry, University of Central Florida, Orlando, FL 32816, United States; College of Science and Liberal Arts, New Jersey Institute of Technology, Newark, NJ 07102, United States.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Belfield
authors.affiliation: Mid-Florida Research and Education Center and Department of Plant Pathology, University of Florida/Institute of Food and Agricultural Sciences, 2725 Binion Rd., Apopka, FL 32703, United States.
authors.forename: David
authors.initials: D
authors.lastname: Norman
authors.affiliation: Mid-Florida Research and Education Center and Department of Plant Pathology, University of Florida/Institute of Food and Agricultural Sciences, 2725 Binion Rd., Apopka, FL 32703, United States.
authors.forename: Mary
authors.initials: M
authors.lastname: Brennan
authors.affiliation: Mid-Florida Research and Education Center and Department of Plant Pathology, University of Florida/Institute of Food and Agricultural Sciences, 2725 Binion Rd., Apopka, FL 32703, United States. Electronic address: gsali@ufl.edu.
authors.forename: Gul Shad
authors.initials: GS
authors.lastname: Ali
mesh_terms: D031171:Artemisia absinthium / Q000737:chemistry*; D055772:Green Chemistry Technology / Q000379:methods*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet; D055672:Static Electricity; D017186:Titrimetry
chemical_list: D010936:Plant Extracts; D012834:Silver
keywords: AgNPs; Artemisia; DLS; EDX