title: Reduction of D-lactate content in sauerkraut using starter cultures of recombinant Leuconostoc mesenteroides expressing the ldhL gene.
abstract: The D-form of lactate, which causes metabolic stress upon excessive dietary intake, is mainly produced by Leuconostoc sp., the predominant species in sauerkraut. To shift the metabolic flux of d-lactate from pyruvate to l-lactate, we expressed the l-lactate dehydrogenase (ldhL) gene in Leuconostoc mesenteroides ATCC 8293. The ldhL gene from Lactobacillus plantarum was introduced into L. mesenteroides using the shuttle vectors pLeuCM and pLeuCM42. To elevate the expression level of ldhL in L. mesenteroides, the nucleotides for pyruvate kinase promoter were fused to ldhL and cloned into above vectors to construct pLC18pkL and pLC42pkL. As results, introduction of pLC42pkL in L. mesenteroides significantly improved both l-LDH activity and l-lactate productivity during fermentation, decreasing the d-/l-lactate ratio. When used as a starter culture for sauerkraut fermentation, recombinant L. mesenteroides harboring pLC42pkL increased l-lactate concentration and decreased d-lactate concentration compared to the wild type strain. We newly developed a recombinant L. mesenteroides which has high l-lactate dehydrogenase activity and applied this strain to minimize the harmful effect of d-lactate during the sauerkraut fermentation. To the best of our knowledge, we demonstrate for the first time the effective use of recombinant Leuconostoc sp. for quality improvement of fermented foods.
authors.affiliation: Department of Food Science, Yanbian University, Yanji, Jilin 133002, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 361-763, South Korea.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 361-763, South Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Moon
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 361-763, South Korea.
authors.forename: Seung Kee
authors.initials: SK
authors.lastname: Cho
authors.affiliation: Department of Milk Processing Research Team, Korea Yakult, Youngin 446-901, South Korea.
authors.forename: Yu Jin
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 361-763, South Korea.
authors.forename: Soo Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 361-763, South Korea. Electronic address: namsoo@cbnu.ac.kr.
authors.forename: Nam Soo
authors.initials: NS
authors.lastname: Han
mesh_terms: D001937:Brassica / Q000382:microbiology; D005285:Fermentation; D005516:Food Microbiology; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology / Q000235:genetics; D007934:Leuconostoc / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D064688:Metabolic Flux Analysis; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase
keywords: Lactic acid bacteria; Leuconostoc; Sauerkraut; d-/l-Lactate; d-/l-Lactate dehydrogenase