title: High-throughput optofluidic particle profiling with morphological and chemical specificity.
abstract: We present a method for high-throughput optofluidic particle analysis that provides both the morphological and chemical profiles of individual particles in a large heterogeneous population. This method is based on an integration of a time-stretch optical microscope with a submicrometer spatial resolution of 780 nm and a three-color fluorescence analyzer on top of an inertial-focusing microfluidic device. The integrated system can perform image- and fluorescence-based screening of particles with a high throughput of 10,000 particles/s, exceeding previously demonstrated imaging particle analyzers in terms of specificity without sacrificing throughput.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ugawa
authors.forename: Cheng
authors.initials: C
authors.lastname: Lei
authors.forename: Taisuke
authors.initials: T
authors.lastname: Nozawa
authors.forename: Takuro
authors.initials: T
authors.lastname: Ideguchi
authors.forename: Dino
authors.initials: D
authors.lastname: Di Carlo
authors.forename: Sadao
authors.initials: S
authors.lastname: Ota
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ozeki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Goda
mesh_terms: D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy; D055620:Optical Phenomena; D013050:Spectrometry, Fluorescence