title: Surface Enhanced Raman Scattering Detection of Cancer Biomarkers with Bifunctional Nanocomposite Probes.
abstract: This report describes new findings of an investigation of a bifunctional nanocomposite probe for the detection of cancer biomarkers, demonstrating the viability of magnetic focusing and SERS detection in a microfluidic platform. The nanocomposite probe consists of a magnetic nickel-iron core and a gold shell. Upon bioconjugation, the nanoprobes are magnetically focused on a specific spot in a microfluidic channel, enabling an enrichment of "hot spots" for surface enhanced Raman scattering detection of the targeted carcinoembryonic antigen. The detection sensitivity, with a limit of detection of ∼0.1 pM, is shown to scale with the magnetic focusing time and the nanoparticle size. The latter is also shown to exhibit an excellent agreement between the experimental data and the theoretical simulation. Implications of the findings to the development of rapid and sensitive microfluidic detection of cancer biomarkers are also discussed.
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Zakiya
authors.initials: Z
authors.lastname: Skeete
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Shiyao
authors.initials: S
authors.lastname: Shan
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Shan
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Chemistry, State University of New York at Potsdam , Potsdam, New York 13676, United States.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kurzatkowska
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Quang Minh
authors.initials: QM
authors.lastname: Ngo
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Polina
authors.initials: P
authors.lastname: Holubovska
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Jin
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Chemistry, State University of New York at Potsdam , Potsdam, New York 13676, United States.
authors.forename: Maria
authors.initials: M
authors.lastname: Hepel
authors.affiliation: Department of Chemistry, State University of New York at Binghamton , Binghamton, New York 13902, United States.
authors.forename: Chuan-Jian
authors.initials: CJ
authors.lastname: Zhong
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D013059:Spectrum Analysis, Raman
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes