title: Genetic variability in the natural populations of Lasioderma serricorne (F.) (Coleoptera: Anobiidae), detected by RAPD markers and by esterase isozymes.
abstract: Lasioderma serricorne (F.) is a small cosmopolitan beetle regarded as a destructive pest of several stored products such as grains, flour, spices, dried fruit and tobacco. Chemical insecticides are one of the measures used against the pest. However, intensive insecticide use has resulted in the appearance of resistant insect populations. Therefore, for the elaboration of more effective control programs, it is necessary to know the biological aspects of L. serricorne. Among these aspects, the genetic variability knowledge is very important and may help in the development of new control methods. The objective of this study was to evaluate the genetic variability of 11 natural populations of L. serricorne collected respectively in three and four towns in the states of Paraná and São Paulo, Brazil, using 20 primers random amplified polymorphic DNA (RAPD) and polymorphisms of esterases. These primers produced 352 polymorphic bands. Electrophoretic analysis of esterases allowed the identification of four polymorphic loci (Est-2, Est-4, Est-5 and Est-6) and 18 alleles. Results show that populations are genetically differentiated and there is a high level of genetic variability within populations. The high degree of genetic differentiation is not directly correlated to geographical distance. Thus, our data indicate that movement of infested commodities may contribute to the dissemination of L. serricorne, facilitating gene flow.
authors.affiliation: Department of Cell Biology and Genetics,State University of Maringá,87020-900 Maringá,Paraná,Brazil.
authors.forename: T
authors.initials: T
authors.lastname: Coelho-Bortolo
authors.affiliation: Department of Cell Biology and Genetics,State University of Maringá,87020-900 Maringá,Paraná,Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Mangolin
authors.affiliation: Department of Cell Biology and Genetics,State University of Maringá,87020-900 Maringá,Paraná,Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Lapenta
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics*; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers; D014644:Genetic Variation; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers; D019476:Insect Proteins; D007527:Isoenzymes; D004950:Esterases
keywords: Lasioderma serricorne; genetic structure; genetic variability; polymorphism