title: Evaluation of Effective MMP Inhibitors from Eight Different Brown Algae in Human Fibrosarcoma HT1080 Cells.
abstract: Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that have important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Marine plants are on the rise for their potential to provide natural products that exhibit remarkable health benefits. In this context, brown algae species have been of much interest in the pharmaceutical field with reported instances of isolation of bioactive compounds against tumor growth and MMP activity. In this study, eight different brown algae species were harvested, and their extracts were compared in regard to their anti-MMP effects. According to gelatin zymography results, Ecklonia cava, Ecklonia bicyclis, and Ishige okamurae showed higher inhibitory effects than the other samples on MMP-2 and -9 activity at the concentrations of 10, 50, and 100 μg/mL. However, only I. okamurae was able to regulate the MMP activity through the expression of MMP and tissue inhibitor of MMP observed by mRNA levels. Overall, brown algae species showed to be good sources for anti-MMP agents, while I. okamurae needs to be further studied for its potential to yield pharmaceutical molecules that can regulate MMP-activity through cellular pathways as well as enzymatic inhibition.
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Korea.
authors.forename: Min Joo
authors.initials: MJ
authors.lastname: Bae
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Korea ; Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, Busan 46958, Korea.
authors.forename: Fatih
authors.initials: F
authors.lastname: Karadeniz
authors.affiliation: Department of Organic Material Science and Engineering, Pusan National University, Busan 46241, Korea.
authors.forename: Byul-Nim
authors.initials: BN
authors.lastname: Ahn
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Korea.
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
keywords: MMP-2; TIMP-2; brown algae; matrix metalloproteinase (MMP)-9; tissue inhibitor of MMP (TIMP)-1