title: UCP1 -3826 A>G polymorphism affects weight, fat mass, and risk of type 2 diabetes mellitus in grade III obese patients.
abstract: OBJECTIVE
  We investigated whether or not the UCP1 -3826 A>G polymorphism is associated with obesity and related metabolic disorders in grade III obese patients.
  
  
  METHODS
  150 obese patients (body mass index ≥35 kg/m(2)) who were candidates for bariatric surgery were studied. Weight (kg), body mass index (kg/m(2)); fat free mass (kg), fat mass (kg), energy intake (kcal), level of physical activity, plasma levels of glucose, total cholesterol, low-density lipoprotein, high-density lipoprotein (HDL), triacylglycerols, and the prevalence of comorbidities associated with obesity were collected from medical records. Polymorphism rs1800592 genotyping was performed through allelic discrimination method in real time polymerase chain reaction using the TaqMan predesigned SNP Genotyping Assays kits. The t test was done to determine if genotypes of each polymorphism are associated with anthropometric and body composition variables. Linear regression models were used for age, sex, height, physical activity, and energy intake in weight and body composition variations (P < 0.05).
  
  
  RESULTS
  Among these 150 individuals (47.2 ± 10.5 y, 80% women) the distribution of AA, AG, and GG was 41.3%, 45.3%, and 13.4%, respectively. Weight and body fat were lower in individuals who were carriers of a mutated allele G. It was observed that mutated homozygotes (GG) had a lower frequency of type 2 diabetes mellitus compared with those of wild allele (AA+AG).
  
  
  CONCLUSIONS
  UCP1 -3826 A>G polymorphism is associated with weight, body fat mass, and risk of type 2 diabetes mellitus in obese individuals candidates for bariatric surgery.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Carolina Ferreira
authors.initials: CF
authors.lastname: Nicoletti
authors.affiliation: Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Ana Paula Rus Perez
authors.initials: AP
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Maria Jose Franco
authors.initials: MJ
authors.lastname: Brochado
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Bruno Parenti
authors.initials: BP
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Marcela Augusta de Souza
authors.initials: MA
authors.lastname: Pinhel
authors.affiliation: Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Julio Sergio
authors.initials: JS
authors.lastname: Marchini
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Jose Ernesto
authors.initials: JE
authors.lastname: dos Santos
authors.affiliation: Department of Surgery and Anatomy, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Wilson
authors.initials: W
authors.lastname: Salgado Junior
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Wilson Araujo
authors.initials: WA
authors.lastname: Silva Junior
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. Electronic address: carla@fmrp.usp.br.
authors.forename: Carla Barbosa
authors.initials: CB
authors.lastname: Nonino
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D001835:Body Weight / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics*; D009765:Obesity / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1
keywords: Bariatric surgery; Obesity; Type II diabetes mellitus; UCP1 -3826 A>G polymorphism; Uncoupling proteins 1