title: On the role of a Lipid-Transfer Protein. Arabidopsis ltp3 mutant is compromised in germination and seedling growth.
abstract: Plant Lipid-Transfer Proteins (LTPs) exhibit the ability to reversibly bind/transport lipids in vitro. LTPs have been involved in diverse physiological processes but conclusive evidence on their role has only been presented for a few members, none of them related to seed physiology. Arabidopsis seeds rely on storage oil breakdown to supply carbon skeletons and energy for seedling growth. Here, Arabidopsis ltp3 mutant was analyzed for its ability to germinate and for seedling establishment. Ltp3 showed delayed germination and reduced germination frequency. Seedling growth appeared reduced in the mutant but this growth restriction was rescued by the addition of an exogenous carbon supply, suggesting a defective oil mobilization. Lipid breakdown analysis during seedling growth revealed a differential profile in the mutant compared to the wild type. The involvement of LTP3 in germination and seedling growth and its relationship with the lipid transfer ability of this protein is discussed.
authors.affiliation: a Instituto de Investigaciones Biológicas; Universidad Nacional de Mar del Plata - CONICET ; Mar del Plata, Argentina.
authors.forename: Luciana A
authors.initials: LA
authors.lastname: Pagnussat
authors.affiliation: a Instituto de Investigaciones Biológicas; Universidad Nacional de Mar del Plata - CONICET ; Mar del Plata, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Oyarburo
authors.affiliation: b Centro de Biotecnología Advanta Semillas S.A.I.C. ; Balcarce, Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Cimmino
authors.affiliation: a Instituto de Investigaciones Biológicas; Universidad Nacional de Mar del Plata - CONICET ; Mar del Plata, Argentina.
authors.forename: Marcela L
authors.initials: ML
authors.lastname: Pinedo
authors.affiliation: a Instituto de Investigaciones Biológicas; Universidad Nacional de Mar del Plata - CONICET ; Mar del Plata, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: de la Canal
mesh_terms: D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000378:metabolism*; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D018525:Germination; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism*; D036226:Seedlings / Q000254:growth & development* / Q000378:metabolism
chemical_list: D052179:Antigens, Plant; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D005227:Fatty Acids; D010940:Plant Proteins; C511920:lipid transfer proteins, plant
keywords: lipid transfer protein; oilseed; seed germination; seedling growth