title: Delivery of Small Interfering RNAs to Cells via Exosomes.
abstract: Exosomes are small membrane bound vesicles between 30 and 100 nm in diameter of endocytic origin that are secreted into the extracellular environment by many different cell types. Exosomes play a role in intercellular communication by transferring proteins, lipids, and RNAs to recipient cells.Exosomes from human cells could be used as vectors to provide cells with therapeutic RNAs. Here we describe how exogenous small interfering RNAs may successfully be introduced into various kinds of human exosomes using electroporation and subsequently delivered to recipient cells. Methods used to confirm the presence of siRNA inside exosomes and cells are presented, such as flow cytometry, confocal microscopy, and Northern blot.
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Gothenburg, Sweden.
authors.forename: Jessica
authors.initials: J
authors.lastname: Wahlgren
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Gothenburg, Sweden.
authors.forename: Luisa
authors.initials: L
authors.lastname: Statello
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Gothenburg, Sweden.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Skogberg
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Gothenburg, Sweden.
authors.forename: Esbjörn
authors.initials: E
authors.lastname: Telemo
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Guldhedsgatan 10A, 413 46, Gothenburg, Sweden. hadi.valadi@gu.se.
authors.forename: Hadi
authors.initials: H
authors.lastname: Valadi
mesh_terms: D058621:Blood Buffy Coat / Q000166:cytology; D015152:Blotting, Northern; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002469:Cell Separation; D016503:Drug Delivery Systems / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D055354:Exosomes / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering
keywords: Electroporation; Exosome delivery; Exosome uptake; Exosomes; Extracellular vesicles; Plasma exosomes; Post-transcriptional gene silencing; siRNA delivery