title: Short-term sleep deprivation disrupts the molecular composition of ionotropic glutamate receptors in entorhinal cortex and impairs the rat spatial reference memory.
abstract: Numerous studies reported that sleep deprivation (SD) causes impairment in spatial cognitive performance. However, the molecular mechanisms affected by SD underlying this behavioral phenomenon remain elusive. Here, we focused on the entorhinal cortex (EC), the gateway of the hippocampus, and investigated how SD affected the subunit expression of AMPARs and NMDARs, the main ionotropic glutamategic receptors serving a pivotal role in spatial cognition. In EC, we found 4h SD remarkably reduced surface expression of GluA1, while there was an increase in the surface expression of GluA2 and GluA3. As for NMDARs, SD with short duration significantly reduced the surface expression levels of GluN1 and GluN2B without effect on the GluN2A. In parallel with the alterations in AMPARs and NMDARs, we found the 4h SD impaired rat spatial reference memory as assessed by Morris water maze task. Overall, these data indicate that brief SD differently affects the AMPAR and NMDAR subunit expressions in EC and might consequently disrupt the composition and functional properties of these receptors.
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Meilan
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China. Electronic address: jialiwang@aliyun.com.
authors.forename: Jiali
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Physiology, Collaborative Innovation Center for Brain Science, Third Military Medical University, Chongqing 400038 PR China. Electronic address: zhianhu@aliyun.com.
authors.forename: Zhian
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000818:Animals; D019921:Biotinylation; D015153:Blotting, Western; D004195:Disease Models, Animal; D004644:Emotions / Q000502:physiology; D018728:Entorhinal Cortex / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D008569:Memory Disorders / Q000209:etiology / Q000503:physiopathology*; D009483:Neuropsychological Tests; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012892:Sleep Deprivation / Q000503:physiopathology* / Q000523:psychology; D065852:Spatial Memory / Q000502:physiology*
chemical_list: D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate
keywords: Entorhinal cortex; Ionotropic glutamate receptor; Morris water maze; Sleep deprivation