title: Polyacrylic acids-bovine serum albumin complexation: Structure and dynamics.
abstract: The study of the mixture of BSA with polyacrylic acids at different masses versus pH allowed highlighting the existence of two regimes of weak and strong complexation. These complexes were studied in diluted regime concentration, by turbidimetry, dynamic light scattering (DLS), zeta-potential measurements and nuclear magnetic resonance (NMR). We have followed the pH effect on the structure and properties of the complex. This allowed refining the interpretation of the phase diagram and understanding the observed phenomena. The NMR measurements allowed probing the dynamics of the constituents versus the pH. The computational method was used to precisely determine the electrostatic potential of BSA and how the polyelectrolyte binds to it at different pH.
authors.affiliation: Labeoratoire de Physique de la Matière Molle et Modélisation Electromagnétique, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Université de Tunis El Manar, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Othman
authors.affiliation: Labeoratoire de Physique de la Matière Molle et Modélisation Electromagnétique, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Université de Tunis El Manar, Tunisia. Electronic address: aschi13@yahoo.fr.
authors.forename: Adel
authors.initials: A
authors.lastname: Aschi
authors.affiliation: Labeoratoire de Physique de la Matière Molle et Modélisation Electromagnétique, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Université de Tunis El Manar, Tunisia.
authors.forename: Abdelhafidh
authors.initials: A
authors.lastname: Gharbi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D000067493:Dynamic Light Scattering; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D055672:Static Electricity; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D012710:Serum Albumin, Bovine; C006903:carbopol 940
keywords: Complex phase; Dynamic light scattering; Electrostatic potential; Nuclear magnetic resonance; Turbidity