title: A Comparative Study on the Population Fitness of Three Strains of Nilaparvata lugens (Hemiptera: Delphacidae) Differ in Eye Color-Related Genes.
abstract: The brown planthopper, Nilaparvata lugens (Stål), is a destructive insect pest on rice throughout Asia. As a visible genetic marker, red eye mutant colony of brown planthopper is a valuable material. Here, we established the near-isogenic lines, NIL-BB and NIL-rr, through mating red eye females to brown eye brothers for eight successive generations. Biological experiments showed that NIL-BB had big fitness cost; however, NIL-rr had comparable survival and fertility parameters with BB, a normal laboratory brown planthopper strain. Significantly lower number eggs per female and egg hatchability were the key factors resulting in big fitness cost of NIL-BB. The population trend indexes of BB, NIL-rr, and NIL-BB were 52.18, 43.80, and 4.19, respectively. Real-time PCR study suggested that the poorer fertility of NIL-BB was not mediated by the differential expression of genes relating to oogenesis. The stronger fitness of NIL-rr compared with NIL-BB may be caused by the eye mutant gene or its closely linked genes having stronger compensation ability for reproduction. The comparable fitness of NIL-rr with BB indicated that NIL-rr may be used in field research. The NIL-BB strain with significantly declined fecundity and survival ability can be used as study model for the signal pathways relating to fecundity.
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, China. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China. jincaiwu1952@sina.com.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China.
authors.forename: Baojun
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310000, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
authors.affiliation: School of Plant Protection, Yangzhou University, Yangzhou 225009, China. jincaiwu1952@sina.com.
authors.forename: Jincai
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D054910:Compound Eye, Arthropod / Q000502:physiology*; D005260:Female; D056084:Genetic Fitness; D006430:Hemiptera / Q000235:genetics / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D015993:Life Tables; D008297:Male; D010858:Pigmentation
chemical_list: D019476:Insect Proteins
keywords: Nilaparvata lugens; expression level; eye color; near-isogenic line; population fitness