title: Proteomic identification of alpha-2-HS-glycoprotein as a plasma biomarker of hypopharyngeal squamous cell carcinoma.
abstract: Hypopharyngeal squamous cell carcinoma (HSCC) has very poor prognosis compared with other head and neck squamous cell carcinomas. Late-stage diagnosis of HSCC increases mortality. Therefore, more effective biomarkers for early diagnosis of HSCC are necessary. Unfortunately, appropriate biomarkers for clinical diagnosis and prognosis have not been identified yet. However, recent progresses in quantitative proteomics have offered opportunities to identify plasma proteins as biomarkers for HSCC. In the present study, plasma samples were analyzed by two-dimensional differential gel electrophoresis (2D-DIGE), and differentially expressed proteins were identified by matrix assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 26 proteins representing 12 unique gene products were identified. The up-regulation proteins were alpha-2-HS-glycoprotein (AHSG), complement C4-B, haptoglobin, C-reactive protein, and ceruloplasmin, whereas the down-regulation proteins were serum albumin, angiotensinogen, alpha-1-antichymotrypsin, Ig gamma-3 chain C region, fibrinogen gamma chain, apolipoprotein A-I, and Ig kappa chain C region. Among all the differentially expressed proteins, AHSG was validated by western blot and ELISA. The results were consistent with the data from 2D-DIGE, further suggesting that AHSG may be employed as a potential biomarker for the early diagnosis of HSCC. In summary, this study was the first to use 2D-DIGE and MALDI-TOF/TOF platform to identify the potential plasma biomarkers for HSCC. The plasma AHSG showed great potential for HSCC screening.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China ; Department of Otolaryngology-Head and Neck Surgery, The Fifth People's Hospital of Dongguan Dongguan 523905, Guangdong, China.
authors.forename: Jun-Zheng
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Shui-Wang
authors.initials: SW
authors.lastname: Hu
authors.affiliation: Department of Head and Neck Surgery and Oncology Plastic Surgery, The Affiliated Cancer Hospital of Xiangya Medical School, Central South University Changsha 410013, Hunan, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Peng
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Huai-Hong
authors.initials: HH
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanfang Hospital, Southern Medical University Guangzhou 510515, Guangdong, China.
authors.forename: Xiang-Ping
authors.initials: XP
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis*; D015536:Down-Regulation; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000097:blood / Q000175:diagnosis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation; D060749:alpha-2-HS-Glycoprotein / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D060749:alpha-2-HS-Glycoprotein
keywords: AHSG; Hypopharyngeal squamous cell carcinoma; biomarker; differential in-gel electrophoresis; plasma; proteomics