title: Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment.
abstract: This paper describes the generation of "click-crosslinkable" and "photodegaradable" gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were created by micropatterned light irradiation; the minimum resolution of micropatterning was 10-μm widths for line patterns and 20-μm diameters for circle patterns. Cells were successfully encapsulated in the hydrogels without any loss of viability across a wide concentration range of crosslinker. In contrast, an activated-ester-type photocleavable crosslinker, which we previously used to prepare photodegradable gelatin hydrogels, induced a decrease in cell viability at crosslinker concentrations greater than 1.8 mM. We also observed morphology alteration and better growth of cancer cells in the click-crosslinked photodegradable gelatin hydrogels that included matrigel than in the absence of matrigel. We also demonstrated micropatterning of the hydrogels encapsulating cells and optical cell separation. Both of the cells that remained in the non-irradiated area and the cells collected from the irradiated area maintained their viability.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Tamura
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Fumiki
authors.initials: F
authors.lastname: Yanagawa
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sugiura
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takagi
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Kimio
authors.initials: K
authors.lastname: Sumaru
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Department of Life Science and Biotechnology, Tsukuba, 605-8565, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kanamori
mesh_terms: D001386:Azides / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D057930:Click Chemistry / Q000379:methods*; D003094:Collagen; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004338:Drug Combinations; D005780:Gelatin / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D007797:Laminin; D008027:Light; D008956:Models, Chemical; D015394:Molecular Structure; D010782:Photolysis / Q000528:radiation effects; D011092:Polyethylene Glycols / Q000737:chemistry; D011509:Proteoglycans; D013388:Succinimides / Q000737:chemistry
chemical_list: D001386:Azides; D003432:Cross-Linking Reagents; D004338:Drug Combinations; D020100:Hydrogels; D007797:Laminin; D011509:Proteoglycans; D013388:Succinimides; C059485:matrigel; D011092:Polyethylene Glycols; D005780:Gelatin; D003094:Collagen; C001426:N-hydroxysuccinimide