title: Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice.
abstract: Jasmonic acid (JA) and related metabolites play a key role in plant defense and growth. JA carboxyl methyltransferase (JMT) may be involved in plant defense and development by methylating JA to methyl jasmonate (MeJA) and thus influencing the concentrations of JA and related metabolites. However, no JMT gene has been well characterized in monocotyledon defense and development at the molecular level. After we cloned a rice JMT gene, OsJMT1, whose encoding protein was localized in the cytosol, we found that the recombinant OsJMT1 protein catalyzed JA to MeJA. OsJMT1 is up-regulated in response to infestation with the brown planthopper (BPH; Nilaparvata lugens). Plants in which OsJMT1 had been overexpressed (oe-JMT plants) showed reduced height and yield. These oe-JMT plants also exhibited increased MeJA levels but reduced levels of herbivore-induced JA and jasmonoyl-isoleucine (JA-Ile). The oe-JMT plants were more attractive to BPH female adults but showed increased resistance to BPH nymphs, probably owing to the different responses of BPH female adults and nymphs to the changes in levels of H2 O2 and MeJA in oe-JMT plants. These results indicate that OsJMT1, by altering levels of JA and related metabolites, plays a role in regulating plant development and herbivore-induced defense responses in rice.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.|Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jiancai
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Han
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yonggen
authors.initials: Y
authors.lastname: Lou
mesh_terms: D000085:Acetates / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D060434:Herbivory / Q000235:genetics / Q000502:physiology*; D007532:Isoleucine / Q000031:analogs & derivatives / Q000378:metabolism; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology* / Q000254:growth & development* / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C532883:jasmonoyl-isoleucine; D007532:Isoleucine; C011006:jasmonic acid; C072239:methyl jasmonate; D008780:Methyltransferases; C425043:jasmonic acid carboxyl methyltransferase
keywords: Herbivore-induced plant defense; Nilaparvata lugens; jasmonic acid; jasmonic acid carboxyl methyltransferase; jasmonoyl-isoleucine; methyl jasmonate; rice