title: Comparing Viability of Periodontal Ligament Stem Cells Isolated From Erupted and Impacted Tooth Root.
abstract: OBJECTIVE
  The aim of the study was to compare the viability of periodontal ligament-derived stem/progenitor cells (PDLSCs) from 2 different sources.
  
  
  METHODS
  Periodontal ligament (PDL) tissue was obtained from 20 surgically extracted human third molars and 20 healthy premolars extracted for orthodontic reasons. Periodontal ligament-derived stem/progenitor cells were isolated from 2 different PDL tissue sources and characterized by colony forming unit assay, cell surface marker characterizations, and their osteogenic differentiation potential. To determine cell viability within 2 groups, the colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) metabolic activity assay was used. Data were statistically analyzed using independent t-test by SPSS 16 software (SPSS Inc, Chicago, IL).
  
  
  RESULTS
  According to the MTT assay, the mean viability rate ± standard deviation of PDLSCs in the impacted third molar sample cells was 0.355 ± 0.411 and for erupted premolar sample cells was 0.331 ± 0.556. Based on One-Sample Kolmogorov-Smirnov test, P value for impacted and erupted teeth was 0.954 and 0.863, respectively. No statistical difference was seen between 2 groups. (P value > 0.05) CONCLUSIONS: Our results demonstrated that if surgical aseptic technique is a method employed to maintain asepsis, PDLSCs obtained from impacted and erupted tooth root would have the same viability rate.
authors.affiliation: *Department of Oral and Maxillofacial Surgery, Dental Faculty, Shahid Beheshti University of Medical Science, Tehran †Department of Anatomy and Embryology, Medical Faculty, Hamadan University of Medical Sciences ‡Department of Biostatistics and Epidemiology, Research Center for Modeling of Noncommunicable Diseases, Public Health Faculty, Hamadan University of Medical Sciences, Hamadan §Department of Oral and Maxillofacial Surgery, Maxillofacial Diseases Research Center, Dental Faculty, Mashhad University of Medical Science, Mashhad ||Department of Oral and Maxillofacial Surgery, Dental Faculty, Kurdestan University of Medical Science, Sanandaj ¶Private Practice, Hamadan, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Dalband
authors.forename: Iraj
authors.initials: I
authors.lastname: Amiri
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Soltanian
authors.forename: Mehdi
authors.initials: M
authors.lastname: Gholami
authors.forename: Adell
authors.initials: A
authors.lastname: Khayati
authors.forename: Vahid
authors.initials: V
authors.lastname: Keshmirypor
mesh_terms: D000293:Adolescent; D000328:Adult; D001641:Bicuspid / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D048430:Cell Shape; D002470:Cell Survival / Q000502:physiology; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology; D008964:Molar, Third / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000502:physiology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014078:Tooth Eruption / Q000502:physiology; D014092:Tooth Root / Q000166:cytology*; D014095:Tooth, Impacted / Q000473:pathology*; D055815:Young Adult
chemical_list: D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue