title: Antifungal susceptibility and molecular typing of 115 Candida albicans isolates obtained from vulvovaginal candidiasis patients in 3 Shanghai maternity hospitals.
abstract: In our multicenter study, we studied the distribution of Candida species in vulvovaginal candidiasis patients and investigated antifungal susceptibility profile and genotype of Candida albicans in vaginal swab. A total of 115 Candida albicans strains were detected in 135 clinical isolates. Minimum inhibitory concentration determinations showed that 83% and 81% of the 115 Candida albicans strains were susceptible to fluconazole and voriconazole. Randomly amplified polymorphic DNA analysis (RAPD) was applied to identify clonally related isolates from different patients at the local level. All tested strains were classified into genotype A (77.4%), genotype B (18.3%), and genotype C (4.3%). Genotype A was further classified into five subtypes and genotype B into two subtypes.Candida albicans was the dominant pathogen of vulvovaginal candidiasis, the majority belonging to genotype A in this study. Exposure to azoles is a risk factor for the emergence of azole resistance among Candida albicans isolated from VVC patients.
authors.affiliation: Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China ycmzh2012@163.com.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ying
authors.affiliation: Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China.
authors.forename: Hongju
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, International Peace Maternity and Child Health Hospital, No. 910 Hengshan Road, Shanghai, 200030, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Clinical Laboratory, Shanghai First Maternity and Infant Hospital, No. 536 Changle Road, Shanghai, 200040, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Clinical Laboratory, Obstetrics and Gynecology Hospital of Fudan University, No. 419 Fangxie Road, Shanghai, 200011, China.
authors.forename: Chaoyan
authors.initials: C
authors.lastname: Yue
mesh_terms: D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology*; D002681:China; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D006771:Hospitals, Maternity; D006801:Humans; D008826:Microbial Sensitivity Tests; D019105:Random Amplified Polymorphic DNA Technique; D012189:Retrospective Studies
keywords: Candida albicans; gene homology; random amplified polymorphic DNA