title: Sonic Hedgehog Promotes Neurite Outgrowth of Primary Cortical Neurons Through Up-Regulating BDNF Expression.
abstract: Sonic hedgehog (Shh), a secreted glycoprotein factor, can activate the Shh pathway, which has been implicated in neuronal polarization involving neurite outgrowth. However, little evidence is available about the effect of Shh on neurite outgrowth in primary cortical neurons and its potential mechanism. Here, we revealed that Shh increased neurite outgrowth in primary cortical neurons, while the Shh pathway inhibitor (cyclopamine, CPM) partially suppressed Shh-induced neurite outgrowth. Similar results were found for the expressions of Shh and Patched genes in Shh-induced primary cortical neurons. Moreover, Shh increased the levels of brain-derived neurotrophic factor (BDNF) not only in lysates and in culture medium but also in the longest neurites of primary cortical neurons, which was partially blocked by CPM. In addition, blocking of BDNF action suppressed Shh-mediated neurite elongation in primary cortical neurons. In conclusion, these findings suggest that Shh promotes neurite outgrowth in primary cortical neurons at least partially through modulating BDNF expression.
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Cui
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China. zhang66xj@126.com.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China. zhang66xj@126.com.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China. zhang66xj@126.com.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China. zhang66xj@126.com.
authors.forename: Xiangjian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China.
authors.forename: Junna
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory for Neurology, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, Shijiazhuang, 050000, Hebei, People's Republic of China.|Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang, 050000, Hebei, People's Republic of China.
authors.forename: Yanzhao
authors.initials: Y
authors.lastname: Xie
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002540:Cerebral Cortex / Q000166:cytology*; D053823:Hedgehog Proteins / Q000378:metabolism* / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D000072079:Patched Receptors; D061251:Primary Cell Culture; D011956:Receptors, Cell Surface / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D053823:Hedgehog Proteins; D000072079:Patched Receptors; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; C465570:Shh protein, mouse
keywords: Brain-derived neurotrophic factor; Cortical neuron; Neurite outgrowth; Sonic hedgehog