title: Increased susceptibility of CD4+ T cells from elderly individuals to HIV-1 infection and apoptosis is associated with reduced CD4 and enhanced CXCR4 and FAS surface expression levels.
abstract: BACKGROUND
  Elderly HIV-1 infected individuals progress to AIDS more frequently and rapidly than people becoming infected at a young age. To identify possible reasons for these differences in clinical progression, we performed comprehensive phenotypic analyses of CD4+ T cells from uninfected young and elderly individuals, and examined their susceptibility to HIV-1 infection and programmed death.
  
  
  RESULTS
  Peripheral blood mononuclear cells (PBMCs) from older people contain an increased percentage of central memory and Th17 CD4+ T cells that are main target cells of HIV-1 and strongly reduced proportions of naïve T cells that are poorly susceptible to HIV-1. Unstimulated T cells from elderly individuals expressed higher levels of activation markers, death receptors, and the viral CXCR4 co-receptor than those from young individuals but responded poorly to stimulation. CD4+ T cells from older individuals were highly susceptible to CXCR4- and CCR5-tropic HIV-1 infection but produced significantly lower quantities of infectious virus than cells from young individuals because they were highly prone to apoptosis and thus presumably had a very short life span. The increased susceptibility of T cells from the elderly to HIV-1 infection correlated directly with CXCR4 and inversely with CD4 expression. The levels of apoptosis correlated with the cell surface expression of FAS but not with the expression of programmed death receptor 1 (PD1) or tumor necrosis factor-related apoptosis-inducing ligand (TRAIL).
  
  
  CONCLUSIONS
  Increased levels of activated and highly susceptible HIV-1 target cells, reduced CD4 and enhanced CXCR4 cell surface expression, together with the high susceptibility to FAS-induced programmed cell death may contribute to the rapid CD4+ T cell depletion and accelerated clinical course of infection in elderly HIV-1-infected individuals.
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, 89081, Ulm, Germany. anke.heigele@uni-ulm.de.
authors.forename: Anke
authors.initials: A
authors.lastname: Heigele
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, 89081, Ulm, Germany. simone.joas@uni-ulm.de.
authors.forename: Simone
authors.initials: S
authors.lastname: Joas
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, 89081, Ulm, Germany. kerstin.regensburger@uni-ulm.de.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Regensburger
authors.affiliation: Institute of Molecular Virology, Ulm University Medical Center, 89081, Ulm, Germany. frank.kirchhoff@uni-ulm.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Kirchhoff
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D017209:Apoptosis; D015704:CD4 Antigens / Q000235:genetics*; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology* / Q000821:virology*; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D007156:Immunologic Memory; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics*; D015398:Signal Transduction; D055815:Young Adult; D019014:fas Receptor / Q000235:genetics*
chemical_list: D015704:CD4 Antigens; C491901:FAS protein, human; D008562:Membrane Glycoproteins; D061026:Programmed Cell Death 1 Receptor; D019718:Receptors, CXCR4; D019014:fas Receptor