title: Effect of Protonation on the Secondary Structure and Orientation of Plant Light-Harvesting Complex II Studied by PM-IRRAS.
abstract: The major light-harvesting pigment-protein complex of photosystem II, LHCII, has a crucial role in the distribution of the light energy between the two photosystems, the efficient light capturing and protection of the reaction centers and antennae from overexcitation. In this work direct structural information on the effect of LHCII protonation, which mimics the switch from light-harvesting to photoprotective state of the protein, was revealed by polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS). PM-IRRAS on LHCII monolayers verified that the native helical structure of the protein is preserved in both partly deprotonated (pH 7.8, LHCII) and protonated (pH 5.2, p-LHCII) states. At low surface pressure, 10 mN/m, the orientation of the α-helices in these two LHCII states is different-tilted (θ ≈ 40°) in LHCII and nearly vertical (θ ≈ 90°) in p-LHCII monolayers; the partly deprotonated complex is more hydrophilic than the protonated one and exhibits stronger intertrimer interactions. At higher surface pressure, 30 mN/m, which is typical for biological membranes, the protonation affects neither the secondary structure nor the orientation of the transmembrane α-helices (tilted ∼45° relative to the membrane surface in both LHCII states) but weakens the intermolecular interactions within and/or between the trimers.
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Tonya D
authors.initials: TD
authors.lastname: Andreeva
authors.affiliation: CBMN-Univ. Bordeaux, UMR 5248 , Allée Geoffroy Saint Hilaire, 33600 Pessac, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Castano
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Sashka
authors.initials: S
authors.lastname: Krumova
authors.affiliation: CBMN-Univ. Bordeaux, UMR 5248 , Allée Geoffroy Saint Hilaire, 33600 Pessac, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Lecomte
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences , Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Stefka G
authors.initials: SG
authors.lastname: Taneva
mesh_terms: D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence
chemical_list: D045342:Light-Harvesting Protein Complexes; D045332:Photosystem II Protein Complex