title: Arabidopsis Yak1 protein (AtYak1) is a dual specificity protein kinase.
abstract: Yak1 is a member of dual-specificity Tyr phosphorylation-regulated kinases (DYRKs) that are evolutionarily conserved. The downstream targets of Yak1 and their functions are largely unknown. Here, a homologous protein AtYAK1 was identified in Arabidopsis thaliana and the phosphoprotein profiles of the wild type and an atyak1 mutant were compared on two-dimensional gel following Pro-Q Diamond phosphoprotein gel staining. Annexin1, Annexin2 and RBD were phosphorylated at serine/threonine residues by the AtYak1 kinase. Annexin1, Annexin2 and Annexin4 were also phosphorylated at tyrosine residues. Our study demonstrated that AtYak1 is a dual specificity protein kinase in Arabidopsis that may regulate the phosphorylation status of the annexin family proteins.
authors.affiliation: Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia; Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia. Electronic address: dongjin.kim@kaust.edu.sa.
authors.forename: Dongjin
authors.initials: D
authors.lastname: Kim
authors.affiliation: Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia; Center for Desert Agriculture, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia.
authors.forename: Valentine Otang
authors.initials: VO
authors.lastname: Ntui
authors.affiliation: Cambridge Systems Biology Centre, Department of Biochemistry, The Sanger Building, Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: Nianshu
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia. Electronic address: liming.xiong@kaust.edu.sa.
authors.forename: Liming
authors.initials: L
authors.lastname: Xiong
mesh_terms: D017302:Annexins / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D009154:Mutation; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: D017302:Annexins; D029681:Arabidopsis Proteins; D010750:Phosphoproteins; D013912:Threonine; D014443:Tyrosine; D012694:Serine; D017346:Protein Serine-Threonine Kinases; C000605774:Yak1 protein, Arabidopsis
keywords: Dual specificity protein kinase; Phosphopeptide; Phosphorylation; Serine/threonine protein kinase; Tyrosine protein kinase