title: The Organophosphate Degradation (opd) Island-borne Esterase-induced Metabolic Diversion in Escherichia coli and Its Influence on p-Nitrophenol Degradation.
abstract: In previous studies of the organophosphate degradation gene cluster, we showed that expression of an open reading frame (orf306) present within the cluster in Escherichia coli allowed growth on p-nitrophenol (PNP) as sole carbon source. We have now shown that expression of orf306 in E. coli causes a dramatic up-regulation in genes coding for alternative carbon catabolism. The propionate, glyoxylate, and methylcitrate cycle pathway-specific enzymes are up-regulated along with hca (phenylpropionate) and mhp (hydroxyphenylpropionate) degradation operons. These hca and mhp operons play a key role in degradation of PNP, enabling E. coli to grow using it as sole carbon source. Supporting growth experiments, PNP degradation products entered central metabolic pathways and were incorporated into the carbon backbone. The protein and RNA samples isolated from E. coli (pSDP10) cells grown in (14)C-labeled PNP indicated incorporation of (14)C carbon, suggesting Orf306-dependent assimilation of PNP in E. coli cells.
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Deviprasanna
authors.initials: D
authors.lastname: Chakka
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Ramurthy
authors.initials: R
authors.lastname: Gudla
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Madikonda
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Emmanuel Vijay Paul
authors.initials: EV
authors.lastname: Pandeeti
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Parthasarathy
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
authors.forename: Aparna
authors.initials: A
authors.lastname: Nandavaram
authors.affiliation: From the Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India sdsl@uohyd.ernet.in.
authors.forename: Dayananda
authors.initials: D
authors.lastname: Siddavattam
mesh_terms: D002244:Carbon / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D004950:Esterases / Q000378:metabolism*; D005798:Genes, Bacterial; D009596:Nitrophenols / Q000378:metabolism*; D010755:Organophosphates / Q000378:metabolism*; D010666:Phenylpropionates / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D009596:Nitrophenols; D010755:Organophosphates; D010666:Phenylpropionates; D002244:Carbon; D004950:Esterases; C024836:4-nitrophenol
keywords: Escherichia coli (E. coli); biodegradation; gene expression; gene knock-out; metabolic regulation; microarray