title: Gene expression and functional characterization of serum amyloid P component 2 in rock bream, Oplegnathus fasciatus.
abstract: Mammalian serum amyloid P component (SAP) recognizes a wide range of exogenous pathogenic substances and activates a complementary pathway leading to pathogen clearance. To determine the potential roles of SAP in the fish immune system, SAP (RbSAP2) gene was cloned from ESTs analysis of rock bream (Oplegnathus fasciatus), which consisted of a signal peptide and pentraxin domain. Phylogenetic analysis revealed that the RbSAP2 gene was classified with other known fish SAPs. RbSAP2 was highly expressed in the liver of healthy rock bream. Overall, pathogen exposure led to an induction of RbSAP2 in the liver and spleen, although this effect was not observed in the spleen following infection with Edwardsiella tarda. A high concentration of recombinant RbSAP2 (rRbSAP2) showed lower growth Streptococcus iniae than control in the absence of Ca(2+), whereas E. tarda growth was decreased by high concentration of rRbSAP in the presence of the Ca(2+). These results suggest that RbSAP plays an important role in the immune response against invading pathogens.
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research & Development Institute, 216 Gijanghaean-Ro, Gijang-Eup, Gijang-Gun, Busan, 619-705, Republic of Korea.
authors.forename: Seong Don
authors.initials: SD
authors.lastname: Hwang
authors.affiliation: Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Jin-Sol
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Jo
authors.affiliation: Aquaculture Industry Division, East Sea Fisheries Research Institute, National Fisheries Research & Development Institute, 482 Sacheonhaean-Ro Yeongok-Myeon, Gangneung, Gangwon, 210-861, Republic of Korea.
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Kim
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research & Development Institute, 216 Gijanghaean-Ro, Gijang-Eup, Gijang-Gun, Busan, 619-705, Republic of Korea.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Cho
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research & Development Institute, 216 Gijanghaean-Ro, Gijang-Eup, Gijang-Gun, Busan, 619-705, Republic of Korea.
authors.forename: Bo Young
authors.initials: BY
authors.lastname: Jee
authors.affiliation: Aquatic Life Disease Control Division, National Fisheries Research & Development Institute, 216 Gijanghaean-Ro, Gijang-Eup, Gijang-Gun, Busan, 619-705, Republic of Korea.
authors.forename: Myoung-Ae
authors.initials: MA
authors.lastname: Park
authors.affiliation: Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary / Q000821:virology; D020609:Edwardsiella tarda / Q000254:growth & development / Q000502:physiology; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007113:Immunity, Innate; D007497:Iridoviridae / Q000502:physiology; D010473:Perciformes; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D000683:Serum Amyloid P-Component / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary; D013291:Streptococcus / Q000254:growth & development / Q000502:physiology; D015854:Up-Regulation
chemical_list: D029941:Fish Proteins; D011994:Recombinant Proteins; D000683:Serum Amyloid P-Component
keywords: Edwardsiella tarda; Oplegnathus fasciatus; Pentraxin; Red seabream iridovirus; SAP; Streptococcus iniae