title: Characteristics of human cell line, F2N78, for the production of recombinant antibody in fed-batch and perfusion cultures.
abstract: A human hybrid cell line, F2N78, was developed by somatic fusion of HEK293 and Namalwa cells for the production recombinant biopharmaceutical proteins. In this study, we performed perfusion culture to verify its potential in culture process used for human cell expression platform. Cell viability could be maintained over 90% and high viable cell density was obtained at higher than 1.0 × 10(7) cells/mL by bleeding process in perfusion culture. The cells were adapted well in both culture modes, but there were apparent differences in protein quality. Compared to fed-batch culture, degalactosylated forms such as G0F and G0 as well as Man5 showed no significant increases in perfusion culture. In terms of charge variants, acidic peaks increased, whereas main peaks constantly decreased according to the length of culture period in both methods.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, South Korea.
authors.forename: Joon Serk
authors.initials: JS
authors.lastname: Seo
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Byung Sub
authors.initials: BS
authors.lastname: Min
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Young-Bum
authors.initials: YB
authors.lastname: Kwon
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Jong-Moon
authors.initials: JM
authors.lastname: Cho
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Keun-Hee
authors.initials: KH
authors.lastname: Park
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Yae Ji
authors.initials: YJ
authors.lastname: Yang
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Ki Eun
authors.initials: KE
authors.lastname: Maeng
authors.affiliation: R&D Center, Celltrion, Incheon 406-840, South Korea.
authors.forename: Shin-Jae
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Biological Engineering, Inha University, Incheon 402-751, South Korea. Electronic address: kimdi@inha.ac.kr.
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
mesh_terms: D000906:Antibodies / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002459:Cell Fusion; D002460:Cell Line; D002470:Cell Survival; D057809:HEK293 Cells; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000378:metabolism*; D010477:Perfusion; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins
keywords: F2N78 cells; Fed-batch culture; Glycosylation; Human cells; Perfusion culture