title: Artificial miRNA-mediated down-regulation of two monolignoid biosynthetic genes (C3H and F5H) cause reduction in lignin content in jute.
abstract: Artificial microRNAs (amiRNA) provide a new feature in the gene silencing era. Concomitantly, reducing the amount of lignin in fiber-yielding plants such as jute holds significant commercial and environmental potential, since this amount is inversely proportional to the quality of the fiber. The present study aimed at reducing the lignin content in jute, by introducing amiRNA based vectors for down-regulation of two monolignoid biosynthetic genes of jute, coumarate 3-hydroxylase (C3H) and ferulate 5-hydroxylase (F5H). The transgenic lines of F5H-amiRNA and C3H-amiRNA showed a reduced level of gene expression, which resulted in about 25% reduction in acid insoluble lignin content for whole stem and 12-15% reduction in fiber lignin as compared to the non-transgenic plants. The results indicate successful F5H-amiRNA and C3H-amiRNA transgenesis for lignin reduction in jute. This is likely to have far-reaching commercial implications and economic acceleration for jute producing countries.
authors.affiliation: Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh.
authors.forename: Farhana
authors.initials: F
authors.lastname: Shafrin
authors.affiliation: International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 11006, India.
authors.forename: Sudhanshu Sekhar
authors.initials: SS
authors.lastname: Das
authors.affiliation: International Center for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 11006, India. neeti@icgeb.res.in.
authors.forename: Neeti
authors.initials: N
authors.lastname: Sanan-Mishra
authors.affiliation: Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh. haseena@du.ac.bd.
authors.forename: Haseena
authors.initials: H
authors.lastname: Khan
mesh_terms: D001483:Base Sequence; D032381:Corchorus / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation; D020868:Gene Silencing; D017343:Genes, Plant; D005813:Genes, Synthetic; D005818:Genetic Engineering; D008031:Lignin / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant; D008031:Lignin
keywords: Gene silencing; Jute; Lignin; amiRNA