title: Sperm cryopreservation of lane snapper Lutjanus synagris (Linnaeus, 1758).
abstract: This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C -min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Secretaria da Agricultura e Abastecimento, Ubatuba, SP, BR.
authors.forename: E G
authors.initials: EG
authors.lastname: Sanches
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Secretaria da Agricultura e Abastecimento, Ubatuba, SP, BR.
authors.forename: I R
authors.initials: IR
authors.lastname: Oliveira
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, Secretaria da Agricultura e Abastecimento, Ubatuba, SP, BR.
authors.forename: P C S
authors.initials: PC
authors.lastname: Serralheiro
authors.affiliation: Departamento de Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
authors.forename: V R
authors.initials: VR
authors.lastname: Cerqueira
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D010473:Perciformes / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects*; D013997:Time Factors
chemical_list: D003451:Cryoprotective Agents