title: Poly(ADP-ribosyl)ation as a new posttranslational modification of YB-1.
abstract: Multifunctional Y-box binding protein 1 (YB-1) is actively studied as one of the components of cellular response to genotoxic stress. However, the precise role of YB-1 in the process of DNA repair is still obscure. In the present work we report for the first time new posttranslational modification of YB-1 - poly(ADP-ribosyl)ation, catalyzed by one of the main regulatory enzymes of DNA repair - poly(ADP-ribose)polymerase 1 (PARP1) in the presence of model DNA substrate carrying multiple DNA lesions. Therefore, poly(ADP-ribosyl)ation of YB-1 catalyzed with PARP1, can be stimulated by damaged DNA. The observed property of YB-1 underlines its ability to participate in the DNA repair by its involvement in the regulatory cascades of DNA repair.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia.
authors.forename: Elizaveta E
authors.initials: EE
authors.lastname: Alemasova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia.
authors.forename: Pavel E
authors.initials: PE
authors.lastname: Pestryakov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Sukhanova
authors.affiliation: Institute of Protein Research, RAS, Pushchino, Moscow Region, 142290, Russia; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 829, Université d'Evry Val d'Essonne, Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, Evry, 91025, France.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kretov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia.
authors.forename: Nina A
authors.initials: NA
authors.lastname: Moor
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 829, Université d'Evry Val d'Essonne, Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, Evry, 91025, France.
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Curmi
authors.affiliation: Institute of Protein Research, RAS, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Lev P
authors.initials: LP
authors.lastname: Ovchinnikov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia. Electronic address: lavrik@niboch.nsc.ru.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D004247:DNA / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D004260:DNA Repair; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D009154:Mutation; D009243:NAD / Q000378:metabolism; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D051840:Y-Box-Binding Protein 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; C427203:calf thymus DNA; C499320:PARP1 protein, human; C000611446:PARP2 protein, S cerevisiae; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases
keywords: AP endonuclease 1 (APE1); Multiple DNA lesions; Poly(ADP-ribose) (PAR; Poly(ADP-ribose)polymerase 1 (PARP1); Y-box binding protein 1 (YB-1); pADPr)