title: Long-Range Regulation of V(D)J Recombination.
abstract: Given their essential role in adaptive immunity, antigen receptor loci have been the focus of analysis for many years and are among a handful of the most well-studied genes in the genome. Their investigation led initially to a detailed knowledge of linear structure and characterization of regulatory elements that confer control of their rearrangement and expression. However, advances in DNA FISH and imaging combined with new molecular approaches that interrogate chromosome conformation have led to a growing appreciation that linear structure is only one aspect of gene regulation and in more recent years, the focus has switched to analyzing the impact of locus conformation and nuclear organization on control of recombination. Despite decades of work and intense effort from numerous labs, we are still left with an incomplete picture of how the assembly of antigen receptor loci is regulated. This chapter summarizes our advances to date and points to areas that need further investigation.
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, USA.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Proudhon
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, USA.
authors.forename: Bingtao
authors.initials: B
authors.lastname: Hao
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, USA.
authors.forename: Ramya
authors.initials: R
authors.lastname: Raviram
authors.affiliation: Institut Curie, CNRS UMR3215, INSERM U934, Paris, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Chaumeil
authors.affiliation: Department of Pathology, New York University School of Medicine, New York, USA. Electronic address: jane.skok@nyumc.org.
authors.forename: Jane A
authors.initials: JA
authors.lastname: Skok
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D011401:Promoter Regions, Genetic; D060152:V(D)J Recombination
chemical_list: D007143:Immunoglobulin Heavy Chains
keywords: ATM; Allelic exclusion; CTCF; Homologous pairing; Nuclear organization; Pericentromeric heterochromatin; RAG; V(D)J recombination