title: Epigenetic silencing of the MUPCDH gene as a possible prognostic biomarker for cyst growth in ADPKD.
abstract: Although autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disease, and is characterized by the formation of multiple fluid-filled cysts, which results in renal failure, early diagnosis and treatment of ADPKD have yet to be defined. Herein, we observed that the promoter region of the gene encoding mucin-like protocadherin (MUPCDH) was hypermethylated in the renal tissue of patients with ADPKD compared to non-ADPKD controls. Inversely, MUPCDH was significantly repressed in ADPKD, especially in cyst-lining cells. Our results indicate that aberrant methylation of MUPCDH promoter CpG islands may be negatively correlated with reduced expression level of MUPCDH and that this contributes to abnormal cell proliferation in ADPKD. It suggests that methylation status of MUPCDH promoter can be used as a novel epigenetic biomarker and a therapeutic target in ADPKD.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Yu Mi
authors.initials: YM
authors.lastname: Woo
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Branch of Cancer Genomics, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do, Korea.
authors.forename: Jung-Ah
authors.initials: JA
authors.lastname: Hwang
authors.affiliation: Department of Internal Medicine, Eulji General Hospital, Seoul, 139-892, Korea.
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Hwang
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Sunyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Park
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Hyun Kyung
authors.initials: HK
authors.lastname: Kong
authors.affiliation: Division of Nephrology, Armed Forces Capital Hospital, Seongnam, Korea.
authors.forename: Hayne Cho
authors.initials: HC
authors.lastname: Park
authors.affiliation: Branch of Cancer Genomics, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do, Korea.
authors.forename: Yeon-Su
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Seoul, 140-742, Korea.
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000070585:Cadherin Related Proteins; D015820:Cadherins / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000235:genetics*; D020868:Gene Silencing; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D052177:Kidney Diseases, Cystic / Q000235:genetics*; D016891:Polycystic Kidney, Autosomal Dominant / Q000235:genetics*; D011379:Prognosis; D015203:Reproducibility of Results; D018570:Risk Assessment / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: C413024:CDHR5 protein, human; D000070585:Cadherin Related Proteins; D015820:Cadherins; D005819:Genetic Markers