title: Plant-Based Vaccine Antigen Production.
abstract: The transient and stable expression of potentially therapeutic proteins in plants is a promising tool for the efficient production of vaccines and antibodies at low cost connected with a practically unlimited scale-up. To achieve these goals, two major challenges, inadequate production levels and non-scalable purification technologies, have to be overcome. Here we present and discuss protocols enabling to perform influenza vaccine production by transient expression in tobacco plants, to perform analytical experiments as Western blot, ELISA, and hemagglutination assays and to purify the antigens by classical affinity chromatography and scalable membrane-based Inverse Transition Cycling.
authors.affiliation: Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Stadt Seeland OT, Gatersleben, 06466, Germany.|Department of Plant Cell Biotechnology, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
authors.forename: Hoang Trong
authors.initials: HT
authors.lastname: Phan
authors.affiliation: Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Stadt Seeland OT, Gatersleben, 06466, Germany. conradu@ipk-gatersleben.de.
authors.forename: Udo
authors.initials: U
authors.lastname: Conrad
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000472:pathogenicity; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines
keywords: ELPylation; Hemagglutinin trimer; Influenza vaccine; Membrane-based inverse transition cycling; Plant-derived hemagglutinin