title: Evaluation of the toxicity of α-(phenylselanyl) acetophenone in mice.
abstract: Selenium is an essential micronutrient with several biological roles in the human body, but supra nutritional consumption can cause toxic effects. The potential deleterious effects of organoselenium compounds are controversial. The compound α-(phenylselanyl) acetophenone (PSAP) exhibits antioxidant, antidepressant-like and glutathione peroxidase-like activity, which makes important the elucidation of any toxic effects. Hence, the present study aims to investigate the in vitro toxicity of PSAP in Chinese Hamster ovary cells (through MTT assay) and analyse its genotoxicity using the comet assay in mice leukocytes after acute or chronic treatments, alongside with biochemical analyses. Our results demonstrate that the oral administration of PSAP in acute (1, 5, 10, 50, 200 mg/kg) and chronic (1, 10, 50, 200 mg/kg) doses did not cause genotoxicity. The compound presented cytotoxic effect in the MTT assay just at 500 μM after 24 h of administration and at 250 and 500 μM after 48 and 72 h of administration. According to biochemical analysis, PSAP presented a minor toxic effect by altering δ-ALA-D activity in liver and catalase activity in kidney at the highest tested concentration. Taking together, these data indicate that PSAP has low toxic effects after chronic administration in mice.
authors.affiliation: Centro de Desenvolvimento Tecnológico, Unidade Biotecnologia, Grupo de pesquisa em Neurobiotecnologia, Universidade Federal de Pelotas, UFPel, Pelotas, RS, Brazil.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Casaril
authors.affiliation: Centro de Desenvolvimento Tecnológico, Unidade Biotecnologia, Grupo de pesquisa em Neurobiotecnologia, Universidade Federal de Pelotas, UFPel, Pelotas, RS, Brazil; Laboratório de Síntese Orgânica Limpa, Universidade Federal de Pelotas, UFPel Pelotas, RS, Brazil.
authors.forename: Débora Martins
authors.initials: DM
authors.lastname: Martinez
authors.affiliation: Laboratório de Síntese Orgânica Limpa, Universidade Federal de Pelotas, UFPel Pelotas, RS, Brazil.
authors.forename: Vanesssa Gentil
authors.initials: VG
authors.lastname: Ricordi
authors.affiliation: Laboratório de Síntese Orgânica Limpa, Universidade Federal de Pelotas, UFPel Pelotas, RS, Brazil.
authors.forename: Diego
authors.initials: D
authors.lastname: Alves
authors.affiliation: Laboratório de Síntese Orgânica Limpa, Universidade Federal de Pelotas, UFPel Pelotas, RS, Brazil.
authors.forename: Eder João
authors.initials: EJ
authors.lastname: Lenardão
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Eduarda
authors.initials: E
authors.lastname: Schultze
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Laboratório de Genômica Funcional, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Fabiana Kömmling
authors.initials: FK
authors.lastname: Seixas
authors.affiliation: Centro de Desenvolvimento Tecnológico, Unidade Biotecnologia, Grupo de pesquisa em Neurobiotecnologia, Universidade Federal de Pelotas, UFPel, Pelotas, RS, Brazil. Electronic address: luciellisavegnago@yahoo.com.br.
authors.forename: Lucielli
authors.initials: L
authors.lastname: Savegnago
mesh_terms: D000098:Acetophenones / Q000008:administration & dosage / Q000633:toxicity*; D000284:Administration, Oral; D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000633:toxicity*; D016466:CHO Cells; D002374:Catalase; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D003412:Cricetulus; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000633:toxicity*; D000623:Porphobilinogen Synthase / Q000378:metabolism; D018570:Risk Assessment
chemical_list: D000098:Acetophenones; D000928:Antidepressive Agents; D000975:Antioxidants; D016566:Organoselenium Compounds; C578833:alpha-(phenylselanyl)acetophenone; D002374:Catalase; D000623:Porphobilinogen Synthase
keywords: Cytotoxicity; Genotoxicity; Organoselenium; Selenium