title: Genetic characterization and improved genotyping of the dysferlin-deficient mouse strain Dysf (tm1Kcam).
abstract: BACKGROUND
  Mouse models of dysferlinopathies are valuable tools with which to investigate the pathomechanisms underlying these diseases and to test novel therapeutic strategies. One such mouse model is the Dysf (tm1Kcam) strain, which was generated using a targeting vector to replace a 12-kb region of the dysferlin gene and which features a progressive muscular dystrophy. A prerequisite for successful animal studies using genetic mouse models is an accurate genotyping protocol. Unfortunately, the lack of robustness of currently available genotyping protocols for the Dysf (tm1Kcam) mouse has prevented efficient colony management. Initial attempts to improve the genotyping protocol based on the published genomic structure failed. These difficulties led us to analyze the targeted locus of the dysferlin gene of the Dysf (tm1Kcam) mouse in greater detail.
  
  
  METHODS
  In this study we resequenced and analyzed the targeted locus of the Dysf (tm1Kcam) mouse and developed a novel PCR protocol for genotyping.
  
  
  RESULTS
  We found that instead of a deletion, the dysferlin locus in the Dysf (tm1Kcam) mouse carries a targeted insertion. This genetic characterization enabled us to establish a reliable method for genotyping of the Dysf (tm1Kcam) mouse, and thus has made efficient colony management possible.
  
  
  CONCLUSIONS
  Our work will make the Dysf (tm1Kcam) mouse model more attractive for animal studies of dysferlinopathies.
authors.affiliation: Neuromuscular Research Group, Departments of Neurology and Biomedicine, University and University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Wiktorowicz
authors.affiliation: Neuromuscular Research Group, Departments of Neurology and Biomedicine, University and University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Jochen
authors.initials: J
authors.lastname: Kinter
authors.affiliation: Howard Hughes Medical Institute, Departments of Molecular Physiology and Biophysics, Neurology, and Internal Medicine, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242 USA.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kobuke
authors.affiliation: Howard Hughes Medical Institute, Departments of Molecular Physiology and Biophysics, Neurology, and Internal Medicine, The University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242 USA.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Campbell
authors.affiliation: Neuromuscular Research Group, Departments of Neurology and Biomedicine, University and University Hospital Basel, Petersgraben 4, 4031 Basel, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Sinnreich
keywords: Dysferlin mouse models; Dysferlinopathies; Genotyping protocol; Knock-out; Targeted deletion