title: De novo transcriptome profiling of highly purified human lymphocytes primary cells.
abstract: To help better understand the role of long noncoding RNAs in the human immune system, we recently generated a comprehensive RNA-seq data set using 63 RNA samples from 13 subsets of T (CD4(+) naive, CD4(+) TH1, CD4(+) TH2, CD4(+) TH17, CD4(+) Treg, CD4(+) TCM, CD4(+) TEM, CD8(+) TCM, CD8(+) TEM, CD8(+) naive) and B (B naive, B memory, B CD5(+)) lymphocytes. There were five biological replicates for each subset except for CD8(+) TCM and B CD5(+) populations that included 4 replicates. RNA-Seq data were generated by an Illumina HiScanSQ sequencer using the TruSeq v3 Cluster kit. 2.192 billion of paired-ends reads, 2×100 bp, were sequenced and after filtering a total of about 1.7 billion reads were mapped. Using different de novo transcriptome reconstruction techniques over 500 previously unknown lincRNAs were identified. The current data set could be exploited to drive the functional characterization of lincRNAs, identify novel genes and regulatory networks associated with specific cells subsets of the human immune system.
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Raoul J P
authors.identifier: 0000-0002-2123-6536
authors.initials: RJ
authors.lastname: Bonnal
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Ranzani
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Arrigoni
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Curti
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Panzeri
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Gruarin
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy ; Department of Clinical Sciences and Community Health, Università degli Studi di Milano , Via Festa del Perdono 7, Milano 20122, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Abrignani
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy.
authors.forename: Grazisa
authors.identifier: 0000-0001-6361-311X
authors.initials: G
authors.lastname: Rossetti
authors.affiliation: Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi' , Via F. Sforza 35, Milan 20122, Italy ; Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano , Via Festa del Perdono 7, Milano 20122, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Pagani
mesh_terms: D016175:B-Lymphocyte Subsets; D020869:Gene Expression Profiling; D006801:Humans; D062085:RNA, Long Noncoding; D016176:T-Lymphocyte Subsets; D059467:Transcriptome
chemical_list: D062085:RNA, Long Noncoding