title: Expression of Glutamate and Glutamine Transporter Proteins in Neurovascular Unit Cells In Vitro.
abstract: Glutamine transporter protein SLC1A5 and glutamate transporter protein EAAT2 responsible for cell-cell communication and energetic coupling were studied using in vitro model of multicellular neurovascular unit consisting of astrocytes, neurons, and endotheliocytes under standard conditions and during chemical hypoxia in vitro. Hypoxic damage to the neurovascular unit cells increased the number of SLC1A5-expressing cells and reduced the number of EAAT2-expressing astrocytes. Metabolic uncoupling in the neurovascular unit cells under hypoxic conditions resulted from abnormal expression of glutamine and glutamate transporter proteins, which is indicative of impaired glutamine and glutamate transport.
authors.affiliation: V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Morgun
authors.affiliation: V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia. natalya.kuvacheva@gmail.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Kuvacheva
authors.affiliation: V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: E D
authors.initials: ED
authors.lastname: Khilazheva
authors.affiliation: V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: T E
authors.initials: TE
authors.lastname: Taranushenko
authors.affiliation: V. F. Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Salmina
mesh_terms: D026962:Amino Acid Transport System ASC / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001692:Biological Transport; D001921:Brain; D002450:Cell Communication; D002454:Cell Differentiation; D015687:Cell Hypoxia; D004622:Embryo, Mammalian; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D019807:Iodoacetic Acid / Q000494:pharmacology; D015778:Minor Histocompatibility Antigens; D008954:Models, Biological; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats
chemical_list: D026962:Amino Acid Transport System ASC; D027342:Excitatory Amino Acid Transporter 2; D015778:Minor Histocompatibility Antigens; C493395:Slc1a2 protein, rat; C498849:Slc1a5 protein, rat; D005973:Glutamine; D018698:Glutamic Acid; D019807:Iodoacetic Acid
keywords: cell–cell interactions; hypoxia; neurovascular unit; transport proteins