title: A Butter Aroma Recombinate Activates Human Class-I Odorant Receptors.
abstract: With ∼400 olfactory G protein-coupled receptors (GPCR), humans sensitively perceive ∼230 key aroma compounds as best natural agonists of ∼10000 food volatiles. An understanding of odorant coding, thus, critically depends on the knowledge about interactions of key food aroma chemicals and their mixtures with their cognate receptors. Genetically designed test cell systems enable the screening, deorphaning, and characterization of single odorant receptors (OR). This study shows for the food aroma-specific and quantitative butter aroma recombinate, and its single components, specific in vitro class-I OR activity patterns, as well as the activation of selected OR in a concentration-dependent manner. Recently, chemosensory receptors, especially class-I OR, were demonstrated to be expressed on blood leukocytes, which may encounter foodborne aroma compounds postprandially. This study shows that butter aroma recombinate induced chemotaxis of isolated human neutrophils in a defined gradient, and in a concentration-dependent and pertussis toxin-sensitive manner, suggesting at least a GPCR-mediated activation of blood leukocytes by key food odorants.
authors.affiliation: Deutsche Forschungsanstalt fuer Lebensmittelchemie Leibniz Institut, Lise-Meitner-Strasse 34, 85354 Freising, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Geithe
authors.affiliation: Deutsche Forschungsanstalt fuer Lebensmittelchemie Leibniz Institut, Lise-Meitner-Strasse 34, 85354 Freising, Germany.
authors.forename: Gaby
authors.initials: G
authors.lastname: Andersen
authors.affiliation: Deutsche Forschungsanstalt fuer Lebensmittelchemie Leibniz Institut, Lise-Meitner-Strasse 34, 85354 Freising, Germany.
authors.forename: Agne
authors.initials: A
authors.lastname: Malki
authors.affiliation: Deutsche Forschungsanstalt fuer Lebensmittelchemie Leibniz Institut, Lise-Meitner-Strasse 34, 85354 Freising, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Krautwurst
mesh_terms: D002079:Butter / Q000032:analysis*; D005421:Flavoring Agents / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D009812:Odorants / Q000032:analysis; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D012903:Smell
chemical_list: D005421:Flavoring Agents; D018035:Receptors, Odorant; D002079:Butter
keywords: GPCR; chemoreception; chemotaxis; key food odorants; neutrophils