title: Implementation of Hadamard encoding for rapid multisample analysis in liquid chromatography.
abstract: Multiplexing based on pseudo-binary modulation sequences is known to increase the signal-to-noise ratio. In this work, Hadamard transform multiplexing is used in high-performance liquid chromatography to increase the sample throughput. Using structured modulation sequences to encode and control sample injections in combination with a fitting algorithm to deconvolute the complex data allowed us to evaluate convoluted chromatograms of up to 128 samples containing three and five analytes, respectively, with good accuracy (<2% deviation). In comparison to conventional high-performance liquid chromatography the analysis time could be reduced by 30 and 55%, respectively.
authors.affiliation: Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
authors.forename: Alexander F
authors.initials: AF
authors.lastname: Siegle
authors.affiliation: Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Trapp
keywords: Hadamard transformation; High-throughput screening; Liquid chromatography; Multiplexing