title: Purification and molecular characterization of a novel mannose-specific lectin from Dioclea reflexa hook seeds with inflammatory activity.
abstract: A novel lectin present in Dioclea reflexa seeds (DrfL) was discovered and described in this study. DrfL was purified in a single step by affinity chromatography in a Sephadex G-50 column. The lectin strongly agglutinated rabbit erythrocytes and was inhibited by α-methyl-D-mannoside, D-mannose, and D-glucose. The hemagglutinating activity of DrfL is optimum at pH 5.0-7.0, stable up to 50 °C, and dependent on divalent cations. Similar to other lectins of the subtribe Diocleinae, the analysis by mass spectrometry indicated that DrfL has three chains (α, β, and γ) with masses of 25,562, 12,874, and 12,706 Da, respectively, with no disulfide bonds or glycosylation. DrfL showed inflammatory activity in the paw edema model and exhibited low cytotoxicity against Artemia sp.
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Vanir R
authors.initials: VR
authors.lastname: Pinto-Junior
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Jorge L A
authors.initials: JL
authors.lastname: Correia
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Ronniery I
authors.initials: RI
authors.lastname: Pereira
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Francisco N
authors.initials: FN
authors.lastname: Pereira-Junior
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Mayara Q
authors.initials: MQ
authors.lastname: Santiago
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Vinicius J S
authors.initials: VJ
authors.lastname: Osterne
authors.affiliation: Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Juliana C
authors.initials: JC
authors.lastname: Madeira
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: João B
authors.initials: JB
authors.lastname: Cajazeiras
authors.affiliation: Laboratório de Espectrometria de Massas aplicado a Proteínas (LEMAP), Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Celso S
authors.initials: CS
authors.lastname: Nagano
authors.affiliation: Laboratório de Biologia Molecular Estrutural e Oncogenética (LBMEO), Department of Molecular Biology, Federal University of Paraíba, João Pessoa, PB, Brazil.
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.affiliation: Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Ana M S
authors.initials: AM
authors.lastname: Assreuy
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Kyria S
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas (Biomol-Lab), Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D052598:Dioclea / Q000737:chemistry*; D004487:Edema / Q000139:chemically induced*; D004912:Erythrocytes / Q000187:drug effects; D006384:Hemagglutination / Q000187:drug effects; D018836:Inflammation Mediators / Q000302:isolation & purification / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology*; D051379:Mice; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D011817:Rabbits
chemical_list: D018836:Inflammation Mediators; D037121:Plant Lectins; D008358:Mannose
keywords: Dioclea reflexa; ESI mass spectrometry; inflammatory activity; lectin