title: Comparative study of the efficacy of decellularization treatment of allogenic and xenogeneic nerves as nerve conduits.
abstract: The objective of this study was to compare the results of allogenic and xenogeneic nerve grafts that were treated using decellularization. The sciatic nerves of Sprague-Dawley rats and the median nerves of Japanese white rabbits were decellularized with sodium dodecyl sulfate and Triton X-100 and examined with a scanning electron microscope and immunofluorescence staining. A bridge-graft into the sciatic nerve in Wistar rats was performed with the decellularized nerves (10 mm in length for short-term evaluation; 15 mm in length for long-term evaluation). As a control, an isograft was performed. The specimens were harvested at 4 weeks postoperatively and prepared for immunohistochemistry. Function, electrophysiological and histomorphological analyses were performed to evaluate nerve recovery at 24 weeks postoperatively. The 3-dimensional structure of the basal lamina column, on which the cell adhesion molecules were integrated, was preserved through the decellularization protocols. Limited ED1-positive macrophage invasion was observed, and abundant NF 160-positive axons, which were accompanied by S-100-positive Schwann cells, penetrated through the implanted nerves. The sciatic nerve function and electrophysiological and histomorphological analyses suggest that the xenogeneic nerve graft was statistically indistinguishable from the allogenic nerve graft but slightly inferior to the isograft in supporting the axonal regeneration and functional recovery.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo, 101-0062, Japan.|Department of Orthopaedic Surgery, Sakurakai Hospital, 2-11-8 Senju Sakuragi, Adachi-ku, Tokyo, 120-0045, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Itoh
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takakuda
mesh_terms: D064591:Allografts; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000473:pathology; D064596:Isografts; D008297:Male; D008475:Median Nerve / Q000378:metabolism* / Q000473:pathology / Q000601:surgery* / Q000637:transplantation; D009416:Nerve Regeneration; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds
keywords: allograft; decellularization; nerve regeneration; xenograft