title: New valid spectrofluorimetric method for determination of selected cephalosporins in different pharmaceutical formulations using safranin as fluorophore.
abstract: A new validated spectrofluorimetric method has been developed for the determination of some cephalosporins namely; cefepime, cefaclor, cefadroxil, cefpodoxime and cefexime. The method was based on the reaction of these drugs with safranin in slightly alkaline medium (pH 8.0), to form ion-association complexes. The fluorescent products were extracted into chloroform and their fluorescence intensities were measured at 544-565 nm after excitation at 518-524 nm. The reaction conditions influencing the product formation and stability were investigated and optimized. The relative fluorescence intensity was proportional to the drug concentration in the linear ranges of 0.15-1.35, 0.35-1.25, 0.35-1.25, 0.20-1.44 and 0.20-1.25 μg/mL for cefepime, cefaclor, cefadroxil, cefpodoxime proxetil and cefexime, respectively. The detection limits were 40, 100, 100, 60 and 70 ng/mL, respectively. The performance of the developed method was evaluated in terms of Student's t-test and variance ratio F-test to find out the significance of proposed methods over the reference spectrophotometric method. Various pharmaceutical formulations were successfully analyzed using the proposed method and the results were in good agreement with those of the previously reported methods.
authors.affiliation: Anal. Chem. Dept., Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Sayed M
authors.initials: SM
authors.lastname: Derayea
authors.affiliation: Pharmaceutical Analysis Dept., Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
authors.forename: Hytham M
authors.initials: HM
authors.lastname: Ahmed
authors.affiliation: Pharm. Chem. Dept., Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Osama H
authors.initials: OH
authors.lastname: Abdelmageed
authors.affiliation: Anal. Chem. Dept., Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Haredy
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D002021:Buffers; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D004304:Dosage Forms; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D057230:Limit of Detection; D010619:Phenazines / Q000737:chemistry*; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D002511:Cephalosporins; D004304:Dosage Forms; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D010619:Phenazines; D012997:Solvents; C009195:safranine T
keywords: Cephalosporin; Pharmaceutical analysis; Safranin; Spectrofluorimetry