title: Quantitative Measurements of Type I and Type II Persisters Using ScanLag.
abstract: The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.
authors.affiliation: The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Levin-Reisman
authors.affiliation: The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. nathalieqb@phys.huji.ac.il.
authors.forename: Nathalie Q
authors.initials: NQ
authors.lastname: Balaban
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006801:Humans
chemical_list: D000900:Anti-Bacterial Agents
keywords: Automatic imaging; Bacterial growth; High-throughput measurements; Lag; Persisters; ScanLag; Stationary phase; Type I persistence; Type II persistence