title: Effects of Different Amounts of Supplemental Selenium and Vitamin E on the Incidence of Retained Placenta, Selenium, Malondialdehyde, and Thyronines Status in Cows Treated with Prostaglandin F2α for the Induction of Parturition.
abstract: The incidence of retained placenta (RP) in cows increases in cases of parturition induced by prostaglandin F2α . We analyzed the effects of different doses of supplemental selenium and vitamin E on the incidence of RP, blood selenium, plasma thyronines, and malondialdehyde concentration. Thirty-three clinically healthy, multiparous Holstein-Frisian cows were assigned to 3 groups and supplemented with a single intramuscular injection of sodium selenite (SS) and tocopherol acetate (TAc) between days 250 to 255 of gestation: control-unsupplemented; group A-10 mg SS + 400 mg TAc; group B-20 mg SS + 800 mg TAc. Parturition was induced using PGF2α not before day 275 of gestation. The RP incidence was reduced from 66.7% in the control to 38.2 and 30.8% in groups A and B, respectively. Blood selenium and glutathione peroxidase activity in treated groups were significantly higher compared to control, with no significant difference between groups A and B. Plasma malondialdehyde in group B was significantly lower than that in control and group A, while thyronines levels were not affected. Comparison of RP and non-RP cows, independently of supplement treatment, revealed higher blood selenium and glutathione peroxidase activity and lower MDA and thyroxine in non-RP animals, while triiodothyronine level did not differ.
authors.affiliation: Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
authors.forename: Ivan B
authors.initials: IB
authors.lastname: Jovanović
authors.affiliation: Veterinary Clinic VELVET, Kaplarova 24a, 19350 Knjaževac, Serbia.
authors.forename: Miljan
authors.initials: M
authors.lastname: Veličković
authors.affiliation: Department for Reproduction, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Vuković
authors.affiliation: Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Milanović
authors.affiliation: Department for Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
authors.forename: Olivera
authors.initials: O
authors.lastname: Valčić
authors.affiliation: Department for Pathophysiology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Gvozdić