title: A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase.
abstract: A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK. h.c.hailes@ucl.ac.uk.
authors.forename: D
authors.initials: D
authors.lastname: Baud
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK. h.c.hailes@ucl.ac.uk.
authors.forename: N
authors.initials: N
authors.lastname: Ladkau
authors.affiliation: Almac, Department of Biocatalysis and Isotope Chemistry, 20 Seagoe Industrial Estate, Craivagon, N. Ireland BT63 5QD, UK.
authors.forename: T S
authors.initials: TS
authors.lastname: Moody
authors.affiliation: Department of Biochemical Engineering, University College London, Gordon Street, London WC1H 0AH, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Ward
authors.affiliation: Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, UK. h.c.hailes@ucl.ac.uk.
authors.forename: H C
authors.initials: HC
authors.lastname: Hailes
mesh_terms: D003124:Colorimetry / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D057230:Limit of Detection; D000637:Transaminases / Q000032:analysis*
chemical_list: D000637:Transaminases