title: Conditions optimising on the yield of biomass, total lipid, and valuable fatty acids in two strains of Skeletonema menzelii.
abstract: Polyunsaturated fatty acids (PUFA) and eicosapentaenoic acid (EPA) are essential for the health of aquaculture organisms and human beings. A total of 9 species of diatoms were screened and two strains of Skeletonema menzelii were selected for further study due to their high growth rates, PUFA and EPA contents. The culture conditions for the yield of biomass, total lipid, EPA and PUFA were optimised. The quickest growth rates (0.28-0.41d(-1)) were achieved with moderate supplement of nitrogen, phosphorus, iron and silicon. The accumulation of total lipid (17.85-22.70% of dry weight) benefitted from deprivation of nitrogen, phosphorus and silicon, but a moderate iron supplement. Highest PUFA (38.26-50.48% of total fatty acids) or EPA (14.26-18.39% of total fatty acids) contents were observed under high nitrogen and phosphorus supplement, moderate available iron but deprivation of silicon. These findings make tangible contributions to culture S. menzelii for commercial production of PUFA or EPA.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Xiamin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Qingxi
authors.initials: Q
authors.lastname: Han
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Xiuzhi
authors.initials: X
authors.lastname: Gao
authors.affiliation: School of Marine Science and Technology, Ridley Building, Newcastle University, Newcastle upon Tyne NE1 7RU, England, UK. Electronic address: g.gao@ncl.ac.uk.
authors.forename: Guang
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D018533:Biomass; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D006801:Humans
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D015118:Eicosapentaenoic Acid
keywords: Docosahexaenoic acid (PubChem CID: 445580); Eicosapentaenoic acid (EPA); Eicosapentaenoic acid (PubChem CID: 446284); Growth; Polyunsaturated fatty acids (PUFA); Skeletonema menzelii; Total lipid