title: Identification of Quantitative Trait Loci Associated with the Skeletal Deformity LSK complex in Gilthead Seabream (Sparus aurata L.).
abstract: Morphological abnormalities, especially skeletal deformities, are some of the most important problems affecting gilthead seabream (Sparus aurata L.) aquaculture industry. In this study, a QTL analysis for LSK complex deformity in gilthead seabream is reported. LSK complex is a severe deformity consisting of a consecutive repetition of three vertebral deformities: lordosis, scoliosis, and kyphosis. Seventy-eight offspring from six breeders from a mass-spawning were analyzed: five full-sibling families, three maternal, and two paternal half-sibling families. They had shown a significant association with the LSK complex prevalence in a previous segregation analysis. Fish were genotyped using a set of multiplex PCRs (ReMsa1-13), which includes 106 microsatellite markers. Two methods were used to perform the QTL analysis: a linear regression with the GridQTL software and a linear mixed model with the Qxpak software. A total of 18 QTL were identified. Four of them (QTLSK3, 6, 12, and 14), located in LG5, 8, 17, and 20, respectively, were the most solid ones. These QTL were significant at genome level and showed an extremely large effect (>35%) with both methods. Markers close to the identified QTL showed a strong association with phenotype. Two of these molecular markers (DId-03-T and Bt-14-F) were considered as potential linked-to-this-deformity markers. The detection of these QTL supposes a critical step in the implementation of marker-assisted selection in this species, which could decrease the incidence of this deformity and other related deformities. The identification of these QTL also represents a major step towards the study of the etiology of skeletal deformities in this species.
authors.forename: Davinia
authors.initials: D
authors.lastname: Negrín-Báez
authors.forename: Davinia
authors.initials: D
authors.lastname: Negrín-Báez
authors.forename: Silvia T
authors.initials: ST
authors.lastname: Rodríguez-Ramilo
authors.forename: Juan M
authors.initials: JM
authors.lastname: Afonso
authors.forename: María J
authors.initials: MJ
authors.lastname: Zamorano
mesh_terms: D000820:Animal Diseases; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D005393:Fish Diseases / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D021541:Sea Bream / Q000002:abnormalities* / Q000235:genetics*; D013121:Spinal Curvatures / Q000235:genetics / Q000662:veterinary*