title: Determination of MICs of levofloxacin for Mycobacterium tuberculosis with gyrA mutations.
abstract: The purpose of the present study was to correlate gyrA mutations found in Mycobacterium tuberculosis isolates using the GenoType(®) MTBDRsl assay with minimum inhibitory concentrations of the fluoroquinolone levofloxacin (LVX). Of 123 archived clinical M. tuberculosis isolates evaluated, 93 isolates had an Ala90Val, Ser91Pro, Asp94Ala, Asn/Tyr, Gly or His mutation and 30 were wild-type. Phenotypically, gyrA mutations Ala90Val, Ser91Pro or Asp94Ala showed a low level of resistance to LVX, while Asp94Asn/Tyr, Asp94Gly or Asp94His mutations had high-level resistance.
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: P
authors.initials: P
authors.lastname: Kambli
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: K
authors.initials: K
authors.lastname: Ajbani
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: C
authors.initials: C
authors.lastname: Nikam
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: A
authors.initials: A
authors.lastname: Khillari
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: A
authors.initials: A
authors.lastname: Shetty
authors.affiliation: Section of Pulmonology, Department of Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: Z
authors.initials: Z
authors.lastname: Udwadia
authors.affiliation: Department of Medicine, University of California, San Diego, San Diego, California, USA.
authors.forename: S B
authors.initials: SB
authors.lastname: Georghiou
authors.affiliation: Department of Medicine, University of California, San Diego, San Diego, California, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Rodwell
authors.affiliation: Department of Medicine, University of California, San Diego, San Diego, California, USA.
authors.forename: A
authors.initials: A
authors.lastname: Catanzaro
authors.affiliation: Section of Microbiology, Department of Laboratory Medicine, PD Hinduja National Hospital and Medical Research Centre, Mumbai, India.
authors.forename: C
authors.initials: C
authors.lastname: Rodrigues
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D064704:Levofloxacin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D014376:Tuberculosis / Q000188:drug therapy / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D064704:Levofloxacin; D027081:DNA Gyrase