title: Human polyomavirus type six in respiratory samples from hospitalized children with respiratory tract infections in Beijing, China.
abstract: BACKGROUND
  HPyV6 is a novel human polyomavirus (HPyV), and neither its natural history nor its prevalence in human disease is well known. Therefore, the epidemiology and phylogenetic status of HPyV6 must be systematically characterized.
  
  
  METHODS
  The VP1 gene of HPyV6 was detected with an established TaqMan real-time PCR from nasopharyngeal aspirate specimens collected from hospitalized children with respiratory tract infections. The HPyV6-positive specimens were screened for other common respiratory viruses with real-time PCR assays.
  
  
  RESULTS
  The prevalence of HPyV6 was 1.7 % (15/887), and children ≤ 5 years of age accounted for 80 % (12/15) of cases. All 15 HPyV6-positive patients were coinfected with other respiratory viruses, of which influenza virus A (IFVA) (8/15, 53.3 %) and respiratory syncytial virus (7/15, 46.7 %) were most common. All 15 HPyV6-positive patients were diagnosed with lower respiratory tract infections, and their viral loads ranged from 1.38 to 182.42 copies/μl nasopharyngeal aspirate specimen. The most common symptoms were cough (100 %) and fever (86.7 %). The complete 4926-bp genome (BJ376 strain, GenBank accession number KM387421) was amplified and showed 100 % identity to HPyV6 strain 607a.
  
  
  CONCLUSIONS
  The prevalence of HPyV6 was 1.7 % in nasopharyngeal aspirate specimens from hospitalized children with respiratory tract infections, as analyzed by real-time PCR. Because the coinfection rate was high and the viral load low, it was not possible to establish a correlation between HPyV6 and respiratory diseases.
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China. zhengwenzhi432@126.com.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Zheng
authors.affiliation: Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, 95 Yong An St., Xi-Cheng District, Beijing, 100050, China. weitl1973@sina.com.
authors.forename: Tian-Li
authors.initials: TL
authors.lastname: Wei
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China. mfl0729@126.com.
authors.forename: Fen-Lian
authors.initials: FL
authors.lastname: Ma
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China. yuanalice2014@163.com.
authors.forename: Wu-Mei
authors.initials: WM
authors.lastname: Yuan
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China. zq781116@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, 95 Yong An St., Xi-Cheng District, Beijing, 100050, China. zhangyaxin0@yeah.net.
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, 95 Yong An St., Xi-Cheng District, Beijing, 100050, China. cuihong100@sina.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Cui
authors.affiliation: Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China. zhenglishu2000@sina.com.
authors.forename: Li-Shu
authors.initials: LS
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000068476:Beijing / Q000453:epidemiology; D002648:Child; D002673:Child, Hospitalized; D002675:Child, Preschool; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D009975:Orthomyxoviridae; D010802:Phylogeny; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012136:Respiratory Syncytial Viruses; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral