title: First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens.
abstract: In this study, we isolated and characterized programmed cell death10 (PDCD10), which is known to be related to apoptosis, from rock bream (Oplegnathus fasciatus). The full-length rock bream PDCD10 (RbPDCD10) cDNA (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. Furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other PDCD10 sequences tested. RbPDCD10 was significantly expressed in the liver, RBC (red blood cell), gill, intestine, trunk kidney and spleen. RbPDCD10 gene expression was also examined in several tissues, including the kidney, spleen, liver, and gill, under bacterial and viral challenges. Generally, all of the examined tissues from the fish that were infected with Edwardsiella tarda and the red sea bream iridovirus (RSIV) exhibited significant up-regulations of RbPDCD10 expression compared to the controls. However, RbPDCD10 expression exhibited dramatic down-regulations in all of the examined tissues following injections of Streptococcus iniae, which is major bacterial pathogen that is responsible for mass mortality in rock bream. Our results revealed that rock bream PDCD10 may be involved in the apoptotic regulation of rock bream immune responses.
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Ju-Won
authors.initials: JW
authors.lastname: Kim
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Jeong
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Jin-Sol
authors.initials: JS
authors.lastname: Bae
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Cho
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Jee-Youn
authors.initials: JY
authors.lastname: Hwang
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Mun-Gyeong
authors.initials: MG
authors.lastname: Kwon
authors.affiliation: Pathology Division, National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea.
authors.forename: Jung Soo
authors.initials: JS
authors.lastname: Seo
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea.
authors.forename: Gun-Wook
authors.initials: GW
authors.lastname: Baeck
authors.affiliation: Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, 38 Cheondaegukchi-Gil, Tongyeong, Gyeongnam 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.
authors.forename: Chan-Il
authors.initials: CI
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D004266:DNA Virus Infections / Q000276:immunology*; D020609:Edwardsiella tarda / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005399:Fishes / Q000276:immunology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D006801:Humans; D007109:Immunity / Q000235:genetics; D007497:Iridoviridae / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics; D013290:Streptococcal Infections / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D029941:Fish Proteins; D008565:Membrane Proteins; C495390:PDCD10 protein, human; D011518:Proto-Oncogene Proteins; D029961:Zebrafish Proteins; C542753:pdcd10a protein, zebrafish
keywords: Edwardsiella tarda; Programmed cell death10; Red sea bream iridovirus; Rock bream; Streptococcus iniae