title: Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No.1.
abstract: For transferring the event-specific PCR methods of genetically modified papaya Huanong No.1 to other laboratories, we validated the previous developed PCR assays of Huanong No.1 according to the international standard organization (ISO) guidelines. A total of 11 laboratories participated and returned their test results in this trial. In qualitative PCR assay, the high specificity and limit of detection as low as 0.1% was confirmed. For the quantitative PCR assay, the limit of quantification was as low as 25 copies. The quantitative biases among ten blind samples were within the range between 0.21% and 10.04%. Furthermore, the measurement uncertainty of the quantitative PCR results was calculated within the range between 0.28% and 2.92% for these ten samples. All results demonstrated that the Huanong No.1 qualitative and quantitative PCR assays were creditable and applicable for identification and quantification of GM papaya Huanong No.1 in further routine lab analysis.
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
authors.forename: Jiaojun
authors.initials: J
authors.lastname: Wei
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China. Electronic address: hyle@sjtu.edu.cn.
authors.forename: Huangying
authors.initials: H
authors.lastname: Le
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
authors.forename: Aihu
authors.initials: A
authors.lastname: Pan
authors.affiliation: Institute of Agriculture Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Xu
authors.affiliation: Agro-biotech Research Center, Jinlin Academy of Agricultural Sciences, Changchun 130124, People's Republic of China.
authors.forename: Feiwu
authors.initials: F
authors.lastname: Li
authors.affiliation: GMO Detection Laboratory, Shanghai Entry-Exit Inspection and Quarantine Bureau, 1208 Minsheng Road, Shanghai 200135, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Quan
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Guo
authors.affiliation: National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. Electronic address: yylltt@sjtu.edu.cn.
authors.forename: Litao
authors.initials: L
authors.lastname: Yang
mesh_terms: D029441:Carica / Q000737:chemistry*; D018744:DNA, Plant / Q000032:analysis* / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D018744:DNA, Plant
keywords: GM papaya Huanong No.1; Method validation; Qualitative and quantitative PCR