title: Interleukin-10 gene promoter region polymorphisms are not associated with BCG osteitis in vaccinated infants.
abstract: METHODS
  Complications arising from bacille Calmette-Guérin (BCG) vaccination were recorded in a national register in Finland until 1988. In the period 1960-1988, 222 patients suffered from BCG osteitis.
  
  
  OBJECTIVE
  To evaluate whether single nucleotide polymorphisms (SNPs) in the promoter region of the gene encoding interleukin 10 (IL-10) are associated with BCG osteitis after vaccination in neonates.
  
  
  METHODS
  Blood samples of 132 former BCG osteitis patients now aged 21-49 years were analysed in a controlled study for IL10 rs1800896 (-1082G/A), rs1800871 (-819C/T), rs1800872 (-592C/A) and rs1800890 (-3575T/A) polymorphisms.
  
  
  RESULTS
  The frequencies of genotypes of IL10 rs1800896, rs1800871, rs1800872 and rs1800890, the frequencies of variant genotypes and the frequencies of major or minor alleles did not differ between patients and controls. Furthermore, the frequencies of the eight possible combinations of the three IL10 alleles located close to each other (IL10 rs1800896, IL10 rs1800871 and IL10 rs1800872) were surprisingly similar.
  
  
  CONCLUSIONS
  Our results suggest that polymorphisms of the IL-10 encoding gene do not play a central role in the development of complications due to BCG vaccination, although the IL10 gene, especially IL10 rs1800896 (-1082G/A) polymorphism, is known to be associated with tuberculosis risk in Europeans and North Americans.
authors.affiliation: Center for Child Health Research, University of Tampere and University Hospital, Tampere, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Pöyhönen
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Teräsjärvi
authors.affiliation: Department of Paediatrics, Seinäjoki Central Hospital, Seinäjoki, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Nuolivirta
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Vuononvirta
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Gröndahl-Yli-Hannuksela
authors.affiliation: Department of Paediatrics, University Hospital, Kuopio, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Kröger
authors.affiliation: School of Health Sciences, University of Tampere, Tampere, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Huhtala
authors.affiliation: Department of Paediatrics and Adolescent Medicine, University of Turku and Turku University Hospital, Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Mertsola
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; Department of Clinical Microbiology, University of Eastern Finland, Kuopio, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Ilonen
authors.affiliation: Department of Paediatrics and Adolescent Medicine, University of Turku and Turku University Hospital, Turku, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Peltola
authors.affiliation: Center for Child Health Research, University of Tampere and University Hospital, Tampere, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Korppi
authors.affiliation: Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland; Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
authors.forename: Q
authors.initials: Q
authors.lastname: He
mesh_terms: D000328:Adult; D000483:Alleles; D001500:BCG Vaccine / Q000008:administration & dosage / Q000009:adverse effects*; D015331:Cohort Studies; D005260:Female; D005387:Finland; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010000:Osteitis / Q000139:chemically induced* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011446:Prospective Studies; D012306:Risk; D055815:Young Adult
chemical_list: D001500:BCG Vaccine; D016753:Interleukin-10