title: Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze.
abstract: Ixodes ricinus immunosuppressor (Iris) is a tick salivary gland protein derived from I. ricinus. In this study, Iris homolog was identified in the salivary glands of Ixodes persulcatus, which is the specific vector of the Lyme disease agent in Japan. The homolog was named Ipis-1. To investigate the function of Ipis-1, we prepared a recombinant Ipis-1 expressed in COS-7 cells as a rabbit IgG Fc-fused protein (Ipis-1-Ig). Cell proliferation assay and IFN-γ ELISA showed that Ipis-1-Ig inhibits the proliferation and IFN-γ production of bovine peripheral blood mononuclear cells (PBMCs). Notably, Ipis-1-Ig inhibited the cell proliferation and production of IFN-γ in bovine PBMCs even when CD14(+) cells were depleted, suggesting that Ipis could directly interact with T cells and inhibit their functions. In conclusion, Ipis could contribute to the establishment of environments suitable for tick blood feeding and pathogen transmission by suppressing the function of immune cells.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Kochi
authors.initials: K
authors.lastname: Toyomane
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. Electronic address: konnai@vetmed.hokudai.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Ayano
authors.initials: A
authors.lastname: Niwa
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Naftaly
authors.initials: N
authors.lastname: Githaka
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Isezaki
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Hokkaido Institute of Public Health, Sapporo, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Takano
authors.affiliation: National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Ando
authors.affiliation: National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kawabata
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Murata
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002417:Cattle; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology; D018884:Ixodes / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008969:Molecular Sequence Data; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D015843:Serpins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D012471:Salivary Proteins and Peptides; D015843:Serpins
keywords: Cell proliferation; IFN-γ; Immunosuppressant; Ipis; Ixodes persulcatus