title: Influence of Iron Supplementation on DMT1 (IRE)-induced Transport of Lead by Brain Barrier Systems in vivo.
abstract: OBJECTIVE
  To investigate the potential involvement of DMT1 (IRE) protein in the brain vascular system in vivo during Pb exposure.
  
  
  METHODS
  Three groups of male Sprague-Dawley rats were exposed to Pb in drinking water, among which two groups were concurrently administered by oral gavage once every other day as the low and high Fe treatment group, respectively, for 6 weeks. At the same time, the group only supplied with high Fe was also set as a reference. The animals were decapitated, then brain capillary-rich fraction was isolate from cerebral cortex. Western blot method was used to identify protein expression, and RT-PCR to detect the change of the mRNA.
  
  
  RESULTS
  Pb exposure significantly increased Pb concentrations in cerebral cortex. Low Fe dose significantly reduced the cortex Pb levels, However, high Fe dose increased the cortex Pb levels. Interestingly, changes of DMT1 (IRE) protein in brain capillary-rich fraction were highly related to the Pb level, but those of DMT1 (IRE) mRNA were not significantly different. Moreover, the consistent changes in the levels of p-ERK1/2 or IRP1 with the changes in the levels of DMT1 (IRE).
  
  
  CONCLUSIONS
  These results suggest that Pb is transported into the brain through DMT1 (IRE), and the ERK MAPK pathway is involved in DMT1 (IRE)-mediated transport regulation in brain vascular system in vivo.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Dai Zhi
authors.initials: DZ
authors.lastname: An
authors.affiliation: Beijing Vocational College of Agriculture, Beijing 102442, China.
authors.forename: Jun Tao
authors.initials: JT
authors.lastname: Ai
authors.affiliation: Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Hong Juan
authors.initials: HJ
authors.lastname: Fang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Ru Bao
authors.initials: RB
authors.lastname: Sun
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Li Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D027682:Cation Transport Proteins / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D019587:Dietary Supplements; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007501:Iron / Q000008:administration & dosage* / Q000378:metabolism; D007854:Lead / Q000008:administration & dosage / Q000493:pharmacokinetics*; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D027682:Cation Transport Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D007501:Iron; D007854:Lead; D012333:RNA, Messenger; D000099236:Solute Carrier Family 11, Member 2
keywords: Blood-brain barrier; Divalent metal transporter 1; Iron; Lead; MAPK pathway