title: Identification of kinases, phosphatases, and phosphorylation sites in human and porcine spermatozoa.
abstract: Multiple inter-connected signaling pathways, involving kinases and phosphatases, form a framework that controls sperm motility, function, and fertilizing ability. Methods that give a broad view of the proteomic landscape may prove valuable in uncovering new crosstalk connections, as well as in discovering new proteins within this regulatory framework. A multi-immunoblotting strategy was utilized to evaluate this concept on human and porcine spermatozoa samples. In human and porcine spermatozoa, a diversity of kinases were identified including protein kinase A (PKA), protein kinase B (PKB), isoforms of protein kinase C (PKC), calmodulin-dependent kinases (CAMK), casein kinase (CK), and isoforms of glycogen synthase kinase (GSK3). Several phosphatases, such as protein phosphatase (PP)-1, PP2A, PP2C, and mitogen activated protein kinase (MAPK) phosphatase (MKP-1), were identified in human spermatozoa. The phosphorylation epitopes recognized belonged to members of the MAPK family, in addition to α and β isoforms of GSK3 and cAMP response element binding protein (CREB). Proteomic approaches that allow a broad view may aid in understanding the crosstalk between signaling systems in spermatozoal physiology.
authors.affiliation: a Endocrine Physiology Laboratory, AVS Department, Clemson University , Clemson , SC , USA.
authors.forename: Brett R
authors.initials: BR
authors.lastname: Lackey
authors.affiliation: a Endocrine Physiology Laboratory, AVS Department, Clemson University , Clemson , SC , USA.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Gray
mesh_terms: D000818:Animals; D006801:Humans; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology*; D013552:Swine
chemical_list: D010770:Phosphotransferases; D010744:Phosphoric Monoester Hydrolases
keywords: Kinase; phosphatase; phosphorylation; spermatozoa