title: Preanalytical, analytical, gestational and pediatric aspects of the S100B immuno-assays.
abstract: BACKGROUND
  Traumatic brain injury management is a tricky issue in children and pregnant women (due to adverse effects of computer tomography). To facilitate management, we report the main analytical performances and reference ranges for blood tests for the well-established S100B biomarker in under-16 children on a DiaSorin® Liaison XL analyzer and in pregnant women on DiaSorin® Liaison XL and Roche Diagnostics® Cobas e411 analyzers.
  
  
  METHODS
  Serum S100B concentrations were determined by chemiluminescent immunoassay on a DiaSorin® analyzer in a population of 409 healthy children aged 0-16 years and on DiaSorin®/Roche Diagnostics® instruments in a population of 50 pregnant women (one blood sample for each trimester). The analytical performances of both instruments and the influence of blood cells and skin pigmentation on the assay were also studied.
  
  
  RESULTS
  For children, four age-groups emerged, i.e. 0-3 months (mean: 0.97 μg/L; standard deviation (SD): 0.36; 95th percentile: 1.55), 4-9 months (mean: 0.58 μg/L; SD: 0.30; 95th: 1.18), 10-24 months (mean: 0.31 μg/L; SD: 0.12; 95th: 0.54) and 2-16 years (mean: 0.20 μg/L; SD: 0.07; 95th: 0.32). For pregnant women, serum S100B concentrations were similar to defined ranges for adults and not significantly different between trimesters on DiaSorin® (p=0.652)/Roche Diagnostics® (p=0.877) analyzers. We also found S100B expression (protein, total mRNA) in lymphocytes, an influence of skin pigmentation, and good analytical performances for both instruments.
  
  
  CONCLUSIONS
  Data provided here is useful for interpreting serum S100B test results, in terms of preanalytical conditions, analytical performances, pediatric and pregnancy' environment.
authors.forename: Damien
authors.initials: D
authors.lastname: Bouvier
authors.forename: Thomas
authors.initials: T
authors.lastname: Duret
authors.forename: Paul
authors.initials: P
authors.lastname: Rouzaire
authors.forename: Matthieu
authors.initials: M
authors.lastname: Jabaudon
authors.forename: Marion
authors.initials: M
authors.lastname: Rouzaire
authors.forename: Céline
authors.initials: C
authors.lastname: Nourrisson
authors.forename: Céline
authors.initials: C
authors.lastname: Bourgne
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.forename: Bertrand
authors.initials: B
authors.lastname: Evrard
authors.forename: Vincent
authors.initials: V
authors.lastname: Sapin
mesh_terms: D000293:Adolescent; D000070642:Brain Injuries, Traumatic / Q000097:blood / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000592:standards; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D012015:Reference Standards; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D064568:S100 Calcium Binding Protein beta Subunit; C579160:S100B protein, human