title: A sensitive electrochemical DNA biosensor for antineoplastic drug 5-fluorouracil based on glassy carbon electrode modified with poly(bromocresol purple).
abstract: This paper describes an electrochemical sensor for the first time based on poly(bromocresol purple) (P(BCP)) developed to observe the interaction between 5-fluorouracil (5-FU) and fish sperm double strand DNA (dsDNA). The P(BCP) film was electrosynthesized by cyclic voltammetry method on the glassy carbon electrode (GCE). The dsDNA was electrochemically immobilized on the surface of P(BCP) modified GCE and the DNA biosensor was prepared. The interaction mechanism of dsDNA with 5-FU was investigated by differential pulse voltammetry using this biosensor. A decrease in the guanine oxidation peak current of the biosensor was observed after the interaction of dsDNA and 5-FU in 0.5 mol L(-1) acetate buffer (pH 4.8) containing 0.02 mol L(-1) NaCl. The accumulation time and dsDNA concentration were optimized to obtain the best peak current response. Under optimum conditions, the linear response on the guanine signal decreasing curve was observed in the 5-FU concentration range of 1.0-50 mg L(-1). The interaction mechanism between dsDNA and 5-FU was further investigated by UV-vis spectroscopy and viscometer. The results reveal that intercalation is the primary mode of interaction between 5-FU and dsDNA.
authors.affiliation: Dumlupınar University, Faculty of Science and Arts, Department of Biochemistry, Kütahya, Turkey. Electronic address: derya.kzeybek@dpu.edu.tr.
authors.forename: Derya
authors.initials: D
authors.lastname: Koyuncu Zeybek
authors.affiliation: Dumlupınar University, Faculty of Science and Arts, Department of Biochemistry, Kütahya, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Demir
authors.affiliation: Dumlupınar University, Faculty of Science and Arts, Department of Chemistry, Kütahya, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Zeybek
authors.affiliation: Ankara University, Faculty of Science, Department of Chemistry, Ankara, Turkey.
authors.forename: Şule
authors.initials: Ş
authors.lastname: Pekyardımcı
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D001962:Bromcresol Purple / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D005472:Fluorouracil / Q000032:analysis* / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D001962:Bromcresol Purple; D006147:Guanine; D002244:Carbon; D004247:DNA; D005472:Fluorouracil
keywords: 5-fluorouracil; DNA biosensor; DNA–drug interaction; Poly(bromocresol purple); intercalation