title: Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay.
abstract: 5,10,15,20-Tetraphenyl-21H,23H-porphine cobalt flat stacking on the reduced graphene oxide with platinum nanoparticles (PtNPs/CoTPP/rGO) were first synthesized and functionalized with monoclonal rabbit anti-aflatoxin B1 antibody (anti-AFB1) for highly efficient electrochemical immunoassay of aflatoxin B1 (AFB1) in this work. Transmission electron microscopy (TEM), atomic force microscope (AFM) and spectral techniques were employed to characterize the PtNPs/CoTPP/rGO hybrids. Using anti-AFB1-conjugated PtNPs/CoTPP/rGO as the signal-transduction tag, a novel non-enzymatic electrochemical immunosensing system was designed for detection of target AFB1 on the AFB1-bovine serum albumin-functionalized sensing interface. Experimental results revealed that the designed immunoassay could exhibit good electrochemical responses for target analyte and allowed the detection of AFB1 at a concentration as low as 5.0 pg mL(-1) (5.0 ppt). Intra- and inter-assay coefficients of variation were below 10%. Importantly, the methodology was further validated for analyzing naturally contaminated or spiked blank peanut samples with consistent results obtained by AFB1 ELISA kit, thus providing a promising approach for quantitative monitoring of organic pollutants.
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education &Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shu
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education &Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education &Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
authors.forename: Qiaohua
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education &Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
authors.forename: Junyang
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education &Fujian Province), Institute of Nanomedicine and Nanobiosensing, Department of Chemistry, Fuzhou University, Fuzhou 350108, P.R. China.
authors.forename: Dianping
authors.initials: D
authors.lastname: Tang
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis; D003035:Cobalt / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008839:Microelectrodes; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004800:Enzymes, Immobilized; D058726:Nanoconjugates; D010087:Oxides; D011166:Porphyrins; D003035:Cobalt; D010984:Platinum; D006108:Graphite; D009195:Peroxidase