title: Rapana thomasiana hemocyanin modified with ionic liquids with enhanced anti breast cancer activity.
abstract: This is the first study on the surface modification of a hemocyanin from marine snail Rapana thomasiana (RtH) with series of imidazolium-based amino acid ionic liquids [emim][AA]. We monitored the induced by [emim][AA] conformational changes in RtH molecule and evaluated the effect of these ionic liquids (ILs) on the protein thermal stability. The cytotoxicity of all obtained RtH-[emim][AA] complexes was assessed toward breast cancer cells (MCF-7) and murine fibroblasts (3T3). As a whole, even small amounts of the tested ILs altered the secondary structure of RtH. The thermal denaturation of RtH in presence of [emim][AA] displayed multi-component transitions, which were shifted toward lower temperatures in comparison to those estimated for the native RtH. The profiles of the RtH-IL calorimetric curves show a clear dependence on the structure of the added salts. In addition, all RtH-[emim][AA] complexes exhibited an enhanced antiprofilerative activity of toward MCF-7 cells in comparison to that of the native RtH. The best results are observed for RtH-[emim][Leu], RtH-[emim][Trp] or RtH-[emim][Ile], which applied in concentration of 700 μg/mL inhibited the MCF-7 cell viability (for 24h) by 66, 63 and 53%, respectively. In addition, these IL-RtH complexes were less cytotoxic to 3T3 cells, i.e. they exhibited some cell specificity.
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9, 1113 Sofia, Bulgaria. Electronic address: maiag@orgchm.bas.bg.
authors.forename: Maya
authors.initials: M
authors.lastname: Guncheva
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9, 1113 Sofia, Bulgaria.
authors.forename: Krasimira
authors.initials: K
authors.lastname: Paunova
authors.affiliation: Institute of Organic Chemical Technology, West Pomeranian University of Technology Szczecin, Pulaski Str. 10, 70-322 Szczecin, Poland; Department of Inorganic and Analytical Chemistry, West Pomeranian University of Technology Szczecin, Piastow 42, 71-065, Szczecin, Poland.
authors.forename: Paula
authors.initials: P
authors.lastname: Ossowicz
authors.affiliation: Department of Inorganic and Analytical Chemistry, West Pomeranian University of Technology Szczecin, Piastow 42, 71-065, Szczecin, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Rozwadowski
authors.affiliation: Institute of Organic Chemical Technology, West Pomeranian University of Technology Szczecin, Pulaski Str. 10, 70-322 Szczecin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Janus
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9, 1113 Sofia, Bulgaria.
authors.forename: Krassimira
authors.initials: K
authors.lastname: Idakieva
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Svetla
authors.initials: S
authors.lastname: Todinova
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9, 1113 Sofia, Bulgaria.
authors.forename: Yuliana
authors.initials: Y
authors.lastname: Raynova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Veselina
authors.initials: V
authors.lastname: Uzunova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Sonia
authors.initials: S
authors.lastname: Apostolova
authors.affiliation: Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., bl. 21, 1113 Sofia, Bulgaria.
authors.forename: Rumiana
authors.initials: R
authors.lastname: Tzoneva
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 9, 1113 Sofia, Bulgaria.
authors.forename: Denitsa
authors.initials: D
authors.lastname: Yancheva
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001943:Breast Neoplasms; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D049851:Gastropoda; D006433:Hemocyanins / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D052578:Ionic Liquids / Q000138:chemical synthesis / Q000737:chemistry*; D061986:MCF-7 Cells; D051379:Mice; D055550:Protein Stability; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000970:Antineoplastic Agents; D052578:Ionic Liquids; D006433:Hemocyanins