title: In vitro and in vivo biological activities of anthocyanins from Nitraria tangutorun Bobr. fruits.
abstract: Anthocyanins are the main compounds in Nitraria tangutorun Bobr. The enrichment and purification of anthocyanins on macroporous resins were investigated. Regarding anthocyanin purification, static adsorption and desorption were studied. The optimal experimental conditions were the following: resin type: X-5; static adsorption time: 6h; desorption solution: ethanol-water-HCl (80:19:1, V/V/V; pH 1); desorption time: 40min. Furthermore, the in vitro and in vivo biological activities of the anthocyanins were evaluated. The anthocyanins showed ideal scavenging effects on free radicals in vitro, especially on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl free radical (OH). In the animal experiment, blood lipid metabolism of hyperlipidemia rats was regulated by anthocyanin contents. The superoxide dismutase (SOD) activity and the total antioxidant capacity (TAC) of hyperlipidemia rats were also improved by anthocyanins. These results showed that anthocyanins from N. tangutorun Bobr. fruits had potential biological activities in vivo as well as in vitro.
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: Qinghai Red Cross Hospital, Xining 810000, China.
authors.forename: Chenxi
authors.initials: C
authors.lastname: Ding
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiulong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Wencong
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China.
authors.forename: Honglun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China. Electronic address: baibochina@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China; Qinghai Nationalities University, Xining 810007, China; Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province, Xining 810001, China. Electronic address: cxding@nwipb.cas.cn.
authors.forename: Chenxu
authors.initials: C
authors.lastname: Ding
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005609:Free Radicals / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D006949:Hyperlipidemias / Q000188:drug therapy* / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D028461:Zygophyllaceae / Q000737:chemistry*
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D017665:Hydroxyl Radical; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl; D013482:Superoxide Dismutase
keywords: Anthocyanins; Antioxidant activities; Enrichment; In vitro and in vivo; Nitraria tangutorun Bobr.