title: Chemopreventive potential of fungal taxol against 7, 12-dimethylbenz[a]anthracene induced mammary gland carcinogenesis in Sprague Dawley rats.
abstract: Breast cancer is the second most prevalent cancer and foremost global public health problem. The present study was designed to appraise the chemopreventive potential of fungal taxol against 7,12-dimethylbenz[a]anthracene (DMBA) induced mammary gland carcinogenesis in Sprague Dawley rats. After 90 days of tumor induction, fungal and authentic taxol were given intraperitoneally once in a week for four weeks. Infrared thermal imaging analysis, serum biochemical parameters such as lipid peroxidase (LPO), creatinine, enzymic and non enzymic antioxidants, liver markers tests such as alanine transaminase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglycerides (TG) and lipoproteins was analysed. In addition, histopathological observation (breast, kidney and liver), immunohistochemical analysis (p53 and Her2/neu) and western blotting experiments (bcl-2, bax and caspase-9) were performed both in control and experimental animals. In thermal imaging, decreased temperature was observed in rat treated with fungal and authentic taxol when compared to tumor induced rats. The significant decrease in LPO, creatinine, ALT, AST, TC, TG, lipoproteins and increase in enzymic, non-enzymic antioxidants were exemplified in serum of treated groups. Further histopathology, immunohistochemical and western blot analysis (bax, cas-9 and bcl-2) of apoptotic markers in breast tissues clearly showed the anti-carcinogenic property of fungal taxol. Our findings implement that fungal taxol is a potential chemo preventive agent against DMBA induced mammary gland carcinogenesis.
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Kathamuthu
authors.initials: K
authors.lastname: Gokul Raj
authors.affiliation: Department of Radiology, Sree Lakshmi Narayana Institute of Medical Sciences (SLIMS), Puducherry 605502, India.
authors.forename: Ranganathan
authors.initials: R
authors.lastname: Chidambaram
authors.affiliation: Department of Biochemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Krishnamoorthy
authors.initials: K
authors.lastname: Varunkumar
authors.affiliation: Department of Biochemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Vilwanathan
authors.initials: V
authors.lastname: Ravikumar
authors.affiliation: Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India. Electronic address: an_pandi@rediffmail.com.
authors.forename: Mohan
authors.initials: M
authors.lastname: Pandi
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D001940:Breast / Q000473:pathology; D063646:Carcinogenesis / Q000187:drug effects*; D053453:Caspase 9 / Q000378:metabolism; D005260:Female; D005658:Fungi / Q000737:chemistry; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy*; D017239:Paclitaxel / Q000494:pharmacology* / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D053453:Caspase 9; D017239:Paclitaxel; D019253:Proto-Oncogene Proteins c-bcl-2; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C524056:Erbb2 protein, mouse
keywords: Breast cancer; DMBA; Fungal taxol; Thermal imaging