title: A mini-review on Sirtuin activity assays.
abstract: Sirtuins belong to the class III histone deacetylase family with NAD-dependent protein lysine deacylase activities, which has been shown to play important roles in many physiological processes, including the regulation of transcription, DNA repair, metabolism and protein secretion. Small molecules that can modulate Sirtuin activity are important for fundamental studies of Sirtuin biology and drug development. High-throughput assays to screen for small molecule modulators of Sirtuins have been approved to be very useful tools for the development of therapeutics and research tools targeting Sirtuins. In this review, we will summarize the reported assays (during 2000-2015) for Sirtuins and discuss how these assays show different performances for the possibility of screening Sirtuin modulators and the application of high-throughput format screening.
authors.affiliation: School of Basic Medical Science, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Shanggao
authors.initials: S
authors.lastname: Liao
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Lan
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Guizhou Medical University, Guiyang, Guizhou 550004 China; Engineering Research Center for the Development and Application of Ethnic Medicine and TCM, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550004 China. Electronic address: binhe@gmc.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: He
mesh_terms: D000818:Animals; D004260:DNA Repair / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D037761:Sirtuins / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D037761:Sirtuins
keywords: Assays; Deacetylation; Deacylation; Histone deacetylase; Sirtuin