title: Methicillin-Resistant Staphylococcus aureus USA300 Latin American Variant in Patients Undergoing Hemodialysis and HIV Infected in a Hospital in Bogotá, Colombia.
abstract: We aimed to determine the prevalence of MRSA colonization and examine the molecular characteristics of colonizing isolates in patients receiving hemodialysis and HIV-infected in a Colombian hospital. Patients on hemodialysis and HIV-infected were prospectively followed between July 2011 and June 2012 in Bogota, Colombia. Nasal and axillary swabs were obtained and cultured. Colonizing S. aureus isolates were identified by standard and molecular techniques. Molecular typing was performed by using pulse-field gel electrophoresis and evaluating the presence of lukF-PV/lukS-PV by PCR. A total of 29% (n = 82) of HIV-infected and 45.5% (n = 15) of patients on hemodialysis exhibited S. aureus colonization. MSSA/MRSA colonization was observed in 28% and 3.6% of the HIV patients, respectively and in 42.4% and 13.3% of the hemodialysis patients, respectively. Staphylococcal cassette chromosome mec typing showed that four MRSA isolates harbored the type IV cassette, and one type I. In the hemodialysis group, two MRSA isolates were classified as belonging to the USA300-LV genetic lineage. Conversely, in the HIV infected group, no colonizing isolates belonging to the USA300-Latin American Variant (UDA300-LV) lineage were identified. Colonizing isolates recovered from the HIV-infected group belonged to the prevalent hospital-associated clones circulating in Latin America (Chilean [n = 1] and Pediatric [n = 2]). The prevalence of MRSA colonization in the study groups was 3.6% (HIV) and 13.3% (hemodialysis). Surveillance programs should be implemented in this group of patients in order to understand the dynamics of colonization and infection in high-risk patients.
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Marylin
authors.initials: M
authors.lastname: Hidalgo
authors.affiliation: Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia.
authors.forename: Lina P
authors.initials: LP
authors.lastname: Carvajal
authors.affiliation: Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Rincón
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Álvaro A
authors.initials: ÁA
authors.lastname: Faccini-Martínez
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Alba A
authors.initials: AA
authors.lastname: Tres Palacios
authors.affiliation: Dirección de Vigilancia y análisis de Riesgo en Salud Pública, Instituto Nacional de Salud, Bogotá, Colombia.
authors.forename: Marcela
authors.initials: M
authors.lastname: Mercado
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Palomá
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Leidy X
authors.initials: LX
authors.lastname: Rayo
authors.affiliation: Department of Microbiology, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Acevedo
authors.affiliation: Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia.
authors.forename: Jinnethe
authors.initials: J
authors.lastname: Reyes
authors.affiliation: Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia; Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, Texas, United States of America.
authors.forename: Diana
authors.initials: D
authors.lastname: Panesso
authors.affiliation: Hospital Universitario San Ignacio, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Paola
authors.initials: P
authors.lastname: García-Padilla
authors.affiliation: Hospital Universitario San Ignacio, Pontificia Universidad Javeriana, Bogotá, Colombia.
authors.forename: Carlos
authors.initials: C
authors.lastname: Alvarez
authors.affiliation: Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, Colombia; Department of Internal Medicine, Division of Infectious Diseases, University of Texas Medical School at Houston, Houston, Texas, United States of America.
authors.forename: Cesar A
authors.initials: CA
authors.lastname: Arias
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000235:genetics*; D003105:Colombia; D005260:Female; D015658:HIV Infections / Q000453:epidemiology / Q000382:microbiology* / Q000628:therapy; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D008875:Middle Aged; D015995:Prevalence; D006435:Renal Dialysis; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D007956:Leukocidins; C108483:leukocidin S-component protein, Staphylococcus; C074300:LukF protein, Staphylococcus aureus