title: In Vitro and In Vivo Tumor Targeted Photothermal Cancer Therapy Using Functionalized Graphene Nanoparticles.
abstract: Despite the tremendous progress that photothermal therapy (PTT) has recently achieved, it still has a long way to go to gain the effective targeted photothermal ablation of tumor cells. Driven by this need, we describe a new class of targeted photothermal therapeutic agents for cancer cells with pH responsive bioimaging using near-infrared dye (NIR) IR825, conjugated poly(ethylene glycol)-g-poly(dimethylaminoethyl methacrylate) (PEG-g-PDMA, PgP), and hyaluronic acid (HA) anchored reduced graphene oxide (rGO) hybrid nanoparticles. The obtained rGO nanoparticles (PgP/HA-rGO) showed pH-dependent fluorescence emission and excellent near-infrared (NIR) irradiation of cancer cells targeted in vitro to provide cytotoxicity. Using intravenously administered PTT agents, the time-dependent in vivo tumor target accumulation was exactly defined, presenting eminent photothermal conversion at 4 and 8 h post-injection, which was demonstrated from the ex vivo biodistribution of tumors. These tumor environment responsive hybrid nanoparticles generated photothermal heat, which caused dominant suppression of tumor growth. The histopathological studies obtained by H&E staining demonstrated complete healing from malignant tumor. In an area of limited successes in cancer therapy, our translation will pave the road to design stimulus environment responsive targeted PTT agents for the safe eradication of devastating cancer.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Kim
authors.affiliation: School of Pharmacy, Sungkyunkwan University , 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 305-701, Republic of Korea.
authors.forename: Shazid Md
authors.initials: SM
authors.lastname: Sharker
authors.affiliation: School of Pharmacy, Sungkyunkwan University , 300 Cheoncheon-dong, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Insik
authors.initials: I
authors.lastname: In
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D004285:Dogs; D006108:Graphite / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D061985:Madin Darby Canine Kidney Cells; D008689:Methacrylates / Q000737:chemistry / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053761:Nanocomposites / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000628:therapy*; D010789:Phototherapy / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics; D019265:Spectroscopy, Near-Infrared; D014018:Tissue Distribution
chemical_list: D001672:Biocompatible Materials; D008689:Methacrylates; D011092:Polyethylene Glycols; D006108:Graphite; D006820:Hyaluronic Acid; C049840:2-(dimethylamino)ethyl methacrylate