title: In Vitro Efficacy of Doripenem against Pseudomonas aeruginosa and Acinetobacter baumannii by E-Test.
abstract: OBJECTIVE
  To assess the in vitro efficacy of doripenem against Pseudomonas aeruginosa and Acinetobacter baumannii using Epsilometer strips.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  Department of Microbiology, Army Medical College, Rawalpindi and National University of Sciences and Technology, Islamabad, from May 2014 to September 2014.
  
  
  METHODS
  A total of 60 isolates of Acinetobacter baumannii and Pseudomonas aeruginosa collected from various clinical samples received from Military Hospital were included in the study. The specimens were inoculated onto blood, MacConkey and chocolate agars. The isolates were identified using Gram staining, motility, catalase test, oxidase test and API 20NE (Biomeriux, France). Organisms identified as Acinetobacter baumannii and Pseudomonas aeruginosa were included in the study. Bacterial suspensions equivalent to 0.5 McFarland turbidity standard of the isolates were prepared and applied on Mueller Hinton agar. Epsilometer strip was placed in the center of the plate and incubated for 18-24 hours. Minimum Inhibitory Concentration (MIC) was taken to be the point where the epsilon intersected the E-strip. MIC of all the isolates was noted.
  
  
  RESULTS
  For Pseudomonas aeruginosa isolates, MIC(50) was 12 µg/mL and MIC(90) was 32 µg/mL. For Acinetobacter baumannii MIC(50) and MIC(90) was 32 µg/mL.
  
  
  CONCLUSIONS
  Doripenem is no more effective against Pseudomonas aeruginosa and Acinetobacter baumannii in our setting.
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Mehreen
authors.initials: M
authors.lastname: Gilani
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Tehmina
authors.initials: T
authors.lastname: Munir
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Mahwish
authors.initials: M
authors.lastname: Latif
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Sabahat
authors.initials: S
authors.lastname: Rehman
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Maliha
authors.initials: M
authors.lastname: Ansari
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Amira
authors.initials: A
authors.lastname: Hafeez
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Sara
authors.initials: S
authors.lastname: Najeeb
authors.affiliation: Department of Microbiology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Nadia
authors.initials: N
authors.lastname: Saad
authors.affiliation: Department of Pathology, Army Medical College, Rawalpindi, (National University of Sciences and Technology), Islamabad.
authors.forename: Mehwish
authors.initials: M
authors.lastname: Gilani
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015780:Carbapenems / Q000494:pharmacology* / Q000627:therapeutic use; D003430:Cross-Sectional Studies; D000077726:Doripenem; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D000077726:Doripenem