title: Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.
abstract: Probiotics exhibit beneficial functions for host homeostasis maintenance. We herein investigated the mechanism by which Lactobacillus brevis-derived poly P exhibited a beneficial function. Immunostaining indicated that poly P was captured in the plasma membrane via integrin β1 in Caco2/bbe cells. The uptake of poly P was reduced by the inhibition of integrin β1 as well as caveolin-1, a major component of lipid rafts. The function of poly P, including the induction of HSP27 and enhancement of the intestinal barrier function, was suppressed by the inhibition of caveolin-1, illustrating that the function of poly P was mediated by the endocytic pathway. High-throughput sequencing revealed that poly P induced tumor necrosis factor alpha-induced protein 3, which contributes to cytoprotection, including upregulation of the intestinal barrier function. The present study demonstrates a novel host-probiotic interaction through the uptake of bacterial substance into host cells, which is distinct from pattern recognition receptor pathways.
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan. Electronic address: fjym@asahikawa-med.ac.jp.
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Fujiya
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Konishi
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ueno
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Kashima
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Junpei
authors.initials: J
authors.lastname: Sasajima
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Moriichi
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Ikuta
authors.affiliation: Department of Gastroenterology, International Health and Science University Hospital, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tanabe
authors.affiliation: Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, Japan; Department of Gastroenterology, International Health and Science University Hospital, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kohgo
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D022461:Caveolins / Q000378:metabolism*; D004705:Endocytosis; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011122:Polyphosphates / Q000494:pharmacology*; D019936:Probiotics / Q000737:chemistry*
chemical_list: D022461:Caveolins; D011122:Polyphosphates
keywords: Host-probiotic interaction; Polyphosphate; Probiotics