title: Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis.
abstract: Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. This suggests that expression of these β-1,3-glucanases is potentially controlled by a single regulatory mechanism.
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Niu
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Wenming
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Mingmei
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Yuanjing
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Pei
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Life Science, Nanjing Normal University , 1 Wenyuan Road, Xianlin University Park, Nanjing, 210023 People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yuan
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D001329:Autolysis; D048690:Fruiting Bodies, Fungal / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation
chemical_list: D005656:Fungal Proteins; D006026:Glycoside Hydrolases
keywords: Coprinopsis cinerea; fruiting body; pileus autolysis; pileus expansion; β-1,3-glucoside hydrolase