title: In Vivo Screening of Traditional Medicinal Plants for Neuroprotective Activity against Aβ42 Cytotoxicity by Using Drosophila Models of Alzheimer's Disease.
abstract: Alzheimer's disease (AD) is the most common neurodegenerative disorder, characterized by progressive neuronal loss with amyloid β-peptide (Aβ) plaques. Despite several drugs currently used to treat AD, their beneficial effects on AD progress remains under debate. Here, we established a rapid in vivo screening system using Drosophila AD models to assess the neuroprotective activities of medicinal plants that have been used in traditional Chinese medicine. Among 23 medicinal plants tested, the extracts from five plants, Coriandrum sativum, Nardostachys jatamansi, Polygonum multiflorum (P. multiflorum), Rehmannia glutinosa, and Sorbus commixta (S. commixta), showed protective effects against the Aβ42 neurotoxicity. We further characterized the neuroprotective activity of ethanol extracts from P. multiflorum and S. commixta. Aβ42-expressing flies that we used showed AD neurological phenotypes, such as decreased survival and motility and increased cell death and reactive oxygen species level. However, feeding these flies extracts from P. multiflorum or S. commixta showed strong suppression of such phenotypes. Similar results were observed in human cells, so that the treatment of P. multiflorum and S. commixta extracts increased the viability of Aβ-treated SH-SY5Y cells. Moreover, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, one of the main constituents of P. multiflorum, also showed similar protective activity against Aβ42 cytotoxicity in both Drosophila and human cells. Taken together, our results suggest that both P. multiflorum and S. commixta have therapeutic potential for the treatment of neurodegenerative diseases, such as AD.
authors.affiliation: Department of Oriental Medicine, Dongguk University.
authors.forename: Quan Feng
authors.initials: QF
authors.lastname: Liu
authors.forename: Jang Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Soojin
authors.initials: S
authors.lastname: Lee
authors.forename: Yoon Ki
authors.initials: YK
authors.lastname: Hong
authors.forename: Soojin
authors.initials: S
authors.lastname: Hwang
authors.forename: Youngje
authors.initials: Y
authors.lastname: Oh
authors.forename: Kyungho
authors.initials: K
authors.lastname: Lee
authors.forename: Hye Sup
authors.initials: HS
authors.lastname: Yun
authors.forename: Im-Soon
authors.initials: IS
authors.lastname: Lee
authors.forename: Songhee
authors.initials: S
authors.lastname: Jeon
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Koo
authors.forename: Kyoung Sang
authors.initials: KS
authors.lastname: Cho
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D000818:Animals; D028526:Coriandrum / Q000737:chemistry; D004195:Disease Models, Animal; D004330:Drosophila; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000068676:Fallopia multiflora / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D032404:Nardostachys / Q000737:chemistry; D018696:Neuroprotective Agents / Q000494:pharmacology*; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D031994:Sorbus / Q000737:chemistry
chemical_list: D016564:Amyloid beta-Protein Precursor; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents