title: Multiscale evaluation of cellular adhesion alteration and cytoskeleton remodeling by magnetic bead twisting.
abstract: Cellular adhesion forces depend on local biological conditions meaning that adhesion characterization must be performed while preserving cellular integrity. We presently postulate that magnetic bead twisting provides an appropriate stress, i.e., basically a clamp, for assessment in living cells of both cellular adhesion and mechanical properties of the cytoskeleton. A global dissociation rate obeying a Bell-type model was used to determine the natural dissociation rate ([Formula: see text]) and a reference stress ([Formula: see text]). These adhesion parameters were determined in parallel to the mechanical properties for a variety of biological conditions in which either adhesion or cytoskeleton was selectively weakened or strengthened by changing successively ligand concentration, actin polymerization level (by treating with cytochalasin D), level of exerted stress (by increasing magnetic torque), and cell environment (by using rigid and soft 3D matrices). On the whole, this multiscale evaluation of the cellular and molecular responses to a controlled stress reveals an evolution which is consistent with stochastic multiple bond theories and with literature results obtained with other molecular techniques. Present results confirm the validity of the proposed bead-twisting approach for its capability to probe cellular and molecular responses in a variety of biological conditions.
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France. daniel.isabey@inserm.fr.
authors.forename: Daniel
authors.initials: D
authors.lastname: Isabey
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.|APHP, Groupe Hospitalier H. Mondor A. Chenevier, Service des Explorations Fonctionnelles, 51, Avenue du Maréchal de Lattre de Tassigny, 94010, Créteil Cedex, France.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Pelle
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.
authors.forename: Sofia
authors.initials: S
authors.lastname: André Dias
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Bottier
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.
authors.forename: Ngoc-Minh
authors.initials: NM
authors.lastname: Nguyen
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.|Physique de la Matière Condensée, Ecole Polytechnique, CNRS, 91128, Palaiseau, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Filoche
authors.affiliation: Inserm, U955, Équipe 13, Biomécanique and Appareil Respiratoire: une approche multi-échelle, UMR S955, CNRS, ERL 7240, Université Paris Est, UPEC, 8, rue du Général Sarrail, 94010, Créteil Cedex, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Louis
mesh_terms: D000072283:A549 Cells; D002448:Cell Adhesion; D003599:Cytoskeleton / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008280:Magnetics / Q000379:methods*; D008863:Microspheres; D008954:Models, Biological
keywords: Clamp; Dissociation rate; Integrin-RGD binding; Multiple bonds; Viscoelastic model