title: Synergistic Effect of Combining Plutella xylostella Granulovirus and Bacillus thuringiensis at Sublethal Dosages on Controlling of Diamondback Moth (Lepidoptera: Plutellidae).
abstract: Plutella xylostella granulovirus (PxGV) and Bacillus thuringiensis (Bt) are both entomo-pathogens to the diamondback moth, Plutella xylostella (L.). The purpose of the present study was to measure the effect of the combination of PxGV and Bt at sublethal dosages on the development and mortality of diamondback moth in a laboratory setting. Bt and PxGV exhibited synergistic effect on diamondback moth larval mortality and effectively controlled diamondback moth populations with low dose combination treatment. The combination of three parts per million Bt and 1.3 × 10(3) occlusion bodies per milliliter of PxGV revealed a higher larval mortality compared with the treatment of Bt or PxGV alone. Combination of Bt and PxGV at sublethal concentrations also increased larval duration, reduced oviposition and decreased adult longevity remarkably, resulting in the lowest population trend index among the treatments. The results suggested that the combination of Bt and PxGV at sublethal dosages might provide a valuable way to improve the control efficacy of diamondback moth compared with treatment of Bt or PxGV alone.
authors.affiliation: Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou 225008, P. R. China. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P. R. China. bio-xj@163.com.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Han
authors.affiliation: Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou 225008, P. R. China. College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, P. R. China.
authors.forename: Chuanming
authors.initials: C
authors.lastname: Li
authors.affiliation: Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou 225008, P. R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou 225008, P. R. China. bio-xj@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000502:physiology*; D025722:Granulovirus / Q000502:physiology*; D007814:Larva / Q000254:growth & development / Q000382:microbiology / Q000821:virology; D009036:Moths / Q000254:growth & development / Q000382:microbiology* / Q000821:virology; D010572:Pest Control, Biological
keywords: Bacillus thuringiensis; Plutella xylostella granulovirus; diamondback moth