title: A biomimetic enzyme modified electrode for H2O2 highly sensitive detection.
abstract: An efficient catalyst based on artificial bionic peroxidase was synthesized for electrocatalysis. A poly(ethyleneimine)/Au nanoparticle composite (PEI-AuNP) was prepared and it was then linked to hemin via a coupling reaction between carboxyl groups in hemin and amino groups in PEI without the activation of a carboxyl group by carbodiimide. Fourier transform infrared (FTIR) spectroscopy verified the formation of amido bonds within the structure. The presence of AuNPs contributed greatly in establishing the amido bonds within the composite. Transmission electron microscopy (TEM) and UV-visible spectroscopy were also used to characterize the PEI-AuNP-hemin catalyst. PEI-AuNP-hemin exhibited intrinsic peroxidase-like catalytic activities. The PEI-AuNP-hemin deposited on a glass carbon electrode had strong sensing for H2O2 with a well-defined linear relationship between the amperometric response and H2O2 concentration in the range from 1 μM to 0.25 mM. The detection limit was 0.247 nM with a high sensitivity of 0.347 mA mM(-1) cm(-2). The peroxidase-like catalytic activity of PEI-AuNP-hemin is discussed in relation to its microstructure. The study suggests that PEI-AuNP-hemin may have promising application prospects in biocatalysis and bioelectronics.
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Jinming
authors.initials: J
authors.lastname: Kong
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Xuehua
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Hu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Hu
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Sailan
authors.initials: S
authors.lastname: Shui
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, P. R. China.
authors.forename: Lianzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, P. R. China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com.
authors.forename: Huifang
authors.initials: H
authors.lastname: Xie
authors.affiliation: School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China. j.kong@njust.edu.cn xueji@usf.edu thwang56@126.com and Chemistry Department, College of Arts and Sciences, University of South Florida, East Fowler Ave, Tampa, Florida 33620-4202, USA.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chemicobiology and Functional Materials Institute, School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China.
authors.forename: Tianhe
authors.initials: T
authors.lastname: Wang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry; D032701:Biomimetics / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D006427:Hemin; D006046:Gold; D011094:Polyethyleneimine; D006861:Hydrogen Peroxide; D009195:Peroxidase