title: Ultra-trace electrochemical impedance determination of bovine serum albumin by a two dimensional silica network citrate-capped gold nanoparticles modified gold electrode.
abstract: In this work, a gold electrode (GE) was modified by coating with two dimensional silica network/citrate capped gold nanoparticles-poly(diallyldimethylammonium chloride) (GE-TDSN-CGNP-PDDA) for ultra-sensitive determination of Bovine Serum Albumin (BSA). After covalently binding of a silica network (in two-dimensional form) on the surface of a gold electrode, via twice in situ hydrolysis of 3-mercaptopropyl-tri-ethoxysilane, citrate capped gold nanoparticles (CGNP) were chemically adsorbed on the silica cage. Subsequently, PDDA was bonded to CGNP via electrostatic interaction of positively charged polymer and negatively charged stabilizer of CGNP. Analytical properties of GE-TDSN-CGNP-PDDA were studied by Electrochemical Impedance Spectroscopy (EIS). The detection limit for measured BSA was found to be 8.4×10(-13) mol L(-1) and the measuring linear concentration range of the proposed sensor was 9.9×10(-12)-1.6×10(-10) mol L(-1) of BSA. In addition, GE-TDSN-CGNP-PDDA exhibited good stability with high selectivity and was applied for determination of BSA in some samples with satisfactory results.
authors.affiliation: Department of Chemistry, Faculty of Science, Lorestan University Kamalvand, 68137-17133 Khorramabad, Iran. Electronic address: a.yari@ymail.com.
authors.forename: Abdollah
authors.initials: A
authors.lastname: Yari
authors.affiliation: Department of Chemistry, Faculty of Science, Lorestan University Kamalvand, 68137-17133 Khorramabad, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Saeidikhah
mesh_terms: D000818:Animals; D002417:Cattle; D019343:Citric Acid / Q000737:chemistry*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D017646:Organosilicon Compounds; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013997:Time Factors
chemical_list: D017646:Organosilicon Compounds; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D012821:Silanes; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012710:Serum Albumin, Bovine; D019343:Citric Acid; D006046:Gold; D012822:Silicon Dioxide; C102833:(3-mercaptopropyl)trimethoxysilane
keywords: Albumin; Bovine; Gold electrode; Impedance spectroscopy; Nanoparticles