title: Expression of Tim-3 in gastric cancer tissue and its relationship with prognosis.
abstract: As a negative regulatory molecule, T-cell immunoglobulin-and mucin domain-3 (Tim-3) plays a crucial role in the tumor immunological tolerance. In the present study, we aimed to determine the Tim-3 expression in gastric cancer tissue and its relationship with clinicopathological parameters and prognosis. The Tim-3 expression was assessed in 52 gastric cancer specimens and 15 gastritis tissues by flow cytometry, and gastritis tissues served as the control. As a result, we found that the Tim-3 expressions on CD4(+)T cells and CD8(+)T cells in gastric cancer tissue was significantly higher than those in gastritis tissue (P=0.022, P=0.047, respectively). The median expression level of Tim-3 on CD4(+)T cells were significantly correlated with clinicopathological parameters, such as tumor size, lymph node metastasis, the depth of tumor invasion and TNM staging (P=0.042, P=0.026, P=0.001, P=0.003, respectively), while it was not correlated with sex, age and histological subtype (all P>0.05). In CD8(+)T cells, the Tim-3 expression was relevant to tumor invasion and TNM staging (P=0.035, P=0.017, respectively), while it was irrelevant to other clinicopathological parameters (all P>0.05). Additionally, Kaplan-Meier survival curves showed that the median overall survival time of patients with lower Tim-3 expression was greater than that of patients with higher Tim-3 expression in CD4(+)T cells and CD8(+)T cells (χ(2)=18.036, P<0.001 and χ(2)=18.036, P<0.001, respectively). Moreover, the multivariate analysis revealed that the Tim-3 expression and TNM stage were independent prognostic factors for gastric cancer patients (P=0.029, P=0.043 and P=0.003, respectively). These results suggest that Tim-3 played an important role in the development and progression of gastric cancer, and it could be used as an independent prognostic factor for gastric cancer patients.
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Gui
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ji
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Fang
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Lujun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Liangrong
authors.initials: L
authors.lastname: Shi
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Changping
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University 185 Juqian Street, Changzhou 213003, P. R. China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D018450:Disease Progression; D005260:Female; D005756:Gastritis / Q000378:metabolism* / Q000473:pathology; D000072597:Hepatitis A Virus Cellular Receptor 2; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012737:Sex Factors; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C448908:HAVCR2 protein, human; D000072597:Hepatitis A Virus Cellular Receptor 2; D008565:Membrane Proteins
keywords: T-cell immunoglobulin-and mucin domain-3 (Tim-3); flow cytometry; gastric cancer; prognosis