title: A rapid high-performance liquid chromatography-tandem mass spectrometry assay for unambiguous detection of different milk species employed in cheese manufacturing.
abstract: The aim of the study was to investigate the possibility to differentiate the 4 most important species in Italian dairy industry (cow, buffalo, sheep, and goat), applying a bottom-up proteomic approach to assess the milk species involved in cheese production. Selective peptides were detected in milk to use as markers in cheese products. Trypsin-digested milk samples of cow, sheep, goat, and buffalo, analyzed by HPLC-tandem mass spectrometry provided species-specific peptides, some of them recognized by Mascot software (Matrix Science Ltd., Boston, MA) as derived from well-known species specific proteins. A multianalyte multiple reaction monitoring method, built with these specific peptides, was successfully applied to cheeses with different composition, showing high specificity in detection of species involved. Neither aging nor production method seemed to affect the response, demonstrating that chosen peptides well act as species markers for dairy products.
authors.affiliation: Real aromi SpA, via Lungofino 3, Città S. Angelo (Pe), Italy 65013.
authors.forename: Nadia
authors.initials: N
authors.lastname: Bernardi
authors.affiliation: Real aromi SpA, via Lungofino 3, Città S. Angelo (Pe), Italy 65013.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Benetti
authors.affiliation: Department of Food Safety, Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via Salvemini 1, Perugia, Italy 06126.
authors.forename: Naceur M
authors.initials: NM
authors.lastname: Haouet
authors.affiliation: Faculty of BioSciences and Technologies for Agriculture Food and Environment, University of Teramo, Italy via C. Lerici 1, Mosciano S.A., Italy 64023.
authors.forename: Manuel
authors.initials: M
authors.lastname: Sergi
authors.affiliation: Faculty of BioSciences and Technologies for Agriculture Food and Environment, University of Teramo, Italy via C. Lerici 1, Mosciano S.A., Italy 64023.
authors.forename: Lisa
authors.initials: L
authors.lastname: Grotta
authors.affiliation: Faculty of BioSciences and Technologies for Agriculture Food and Environment, University of Teramo, Italy via C. Lerici 1, Mosciano S.A., Italy 64023.
authors.forename: Sonia
authors.initials: S
authors.lastname: Marchetti
authors.affiliation: Faculty of BioSciences and Technologies for Agriculture Food and Environment, University of Teramo, Italy via C. Lerici 1, Mosciano S.A., Italy 64023.
authors.forename: Federica
authors.initials: F
authors.lastname: Castellani
authors.affiliation: Faculty of BioSciences and Technologies for Agriculture Food and Environment, University of Teramo, Italy via C. Lerici 1, Mosciano S.A., Italy 64023. Electronic address: gmartino@unite.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Martino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002020:Buffaloes; D002417:Cattle; D002611:Cheese / Q000032:analysis / Q000145:classification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D007558:Italy; D008892:Milk / Q000737:chemistry / Q000145:classification*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D040901:Proteomics; D012756:Sheep; D034561:Sheep, Domestic; D013045:Species Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D010455:Peptides
keywords: HPLC-MS/MS; animal species; cheese; milk; peptides; trypsin