title: Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines.
abstract: Replication-deficient adenoviruses are potent vaccine development platforms used extensively for human and animal candidate vaccines, largely due to their very good safety and immunogenicity profile. In this chapter we describe a method that can be used in any laboratory for the scalable production of replication-deficient adenovirus vector vaccines to GLP for preclinical studies in animal models, including definitive experimental studies in large target animal species for veterinary applications. We use human adenovirus serotype 5 (HAdV5) as an example, but the method can be easily adapted for use with other adenovirus serotypes from different species of origin.
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ, UK. susan.morris@ndm.ox.ac.uk.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Morris
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ, UK.
authors.forename: Alison V
authors.initials: AV
authors.lastname: Turner
authors.affiliation: The Clinical Biomanufacturing Facility, University of Oxford, Churchill Drive, Headington, Oxford, OX3 7JT, UK.
authors.forename: Nicola
authors.initials: N
authors.lastname: Green
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ, UK.
authors.forename: George M
authors.initials: GM
authors.lastname: Warimwe
mesh_terms: D062705:Adenovirus Vaccines / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors / Q000276:immunology / Q000627:therapeutic use; D016679:Genome, Viral; D057809:HEK293 Cells; D006801:Humans; D014611:Vaccination / Q000379:methods*; D014779:Virus Replication / Q000235:genetics
chemical_list: D062705:Adenovirus Vaccines; D000914:Antibodies, Viral
keywords: Adenovirus serotype 5 viral vector; HEK293 cells; Seed stocks; Transfection