title: In vitro anti-HIV-1 activity of fucoidan from Sargassum swartzii.
abstract: Sargassum swartzii, a marine brown algae with wide range of biological properties belongs to the family Sargassaceae. Bioactive fucoidan fractions (CFF, FF1 and FF2) were isolated from S. swartzii and characterized by linear gradient anion-exchange chromatography and FT-IR. The characterized fucoidan fractions contained mainly sugars, sulfate and uronic acid. In the present study, anti-HIV-1 property of the fucoidan fractions was investigated. Fraction FF2 was found to exhibit significant anti-HIV-1 activity at concentrations of 1.56 and 6.25 μg/ml as observed by >50% reduction in HIV-1 p24 antigen levels and reverse transcriptase activity. Fucoidan fractions have no cytotoxic effects on PBMCs at the concentration range of 1.56-1000 μg/ml. These results suggest that fucoidan fractions could have inhibitory activity against HIV and has potential as an anti-HIV-1 agent.
authors.affiliation: Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai, TN, India.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Dinesh
authors.affiliation: Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai, TN, India. Electronic address: thangam56@gmail.com.
authors.forename: Thangam
authors.initials: T
authors.lastname: Menon
authors.affiliation: Division of HIV/AIDS, Department of Clinical Research, National Institute for Research in Tuberculosis (NIRT), Chennai, TN, India.
authors.forename: Luke E
authors.initials: LE
authors.lastname: Hanna
authors.affiliation: Department of Botany, Annamalai University, Annamalai Nagar, TN, India.
authors.forename: V
authors.initials: V
authors.lastname: Suresh
authors.affiliation: Centre for Advanced Studies in Botany, University of Madras, Chennai, TN, India.
authors.forename: M
authors.initials: M
authors.lastname: Sathuvan
authors.affiliation: Centre for Drug Discovery and Development, Sathyabama University, Chennai 600119, India.
authors.forename: M
authors.initials: M
authors.lastname: Manikannan
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry* / Q000494:pharmacology*; D002478:Cells, Cultured; D016655:HIV Core Protein p24 / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019380:Anti-HIV Agents; D016655:HIV Core Protein p24; D011134:Polysaccharides; C514799:p24 protein, Human Immunodeficiency Virus Type 1; C007789:fucoidan; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase
keywords: Fucoidan; HIV-1; Reverse transcriptase; Sargassum swartzii