title: Evaluation of antifertility effect of gel formulation containing sperm immobilizing factor: In vitro and in vivo studies.
abstract: Sexually active women often seek protection against unplanned pregnancies. Latter can be effectively controlled by consistent use of spermicides during each coital act. However, side effects associated with the use of available synthetic spermicidal agents have directed the interest towards identifying newer and safer agents. Present studies were undertaken to formulate a vaginal contraceptive gel, containing sperm immobilizing factor (SIF) isolated from Staphylococcus aureus using 1% w/v Carbopol. SIF loaded gel formulation was characterized for various in vitro parameters i.e. pH, spreadability, texture profiling, rheological properties, and in vitro release studies. The prepared formulation was found to possess significant spreading properties, gel firmness and strength, and released about 80% of SIF within 30min. Latter can completely immobilize human spermatozoa within 20s, at a dose of 200μg/ml. SIF in the proposed gel formulation showed 100% contraceptive effect when used at amount as low as of 10μg, thus confirming the possibility to develop it as a potent vaginal contraceptive for future use.
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh, India.
authors.forename: Sonia
authors.initials: S
authors.lastname: Gupta
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
authors.forename: Indu Pal
authors.initials: IP
authors.lastname: Kaur
authors.affiliation: Department of Microbiology, Panjab University, Chandigarh, India. Electronic address: satishvijay11@yahoo.com.
authors.forename: Vijay
authors.initials: V
authors.lastname: Prabha
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D065546:Drug Liberation; D005260:Female; D005298:Fertility / Q000187:drug effects*; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D012212:Rheology; D013078:Sperm Immobilizing Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013211:Staphylococcus aureus; D014622:Vaginal Creams, Foams, and Jellies / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000180:Acrylic Resins; D013078:Sperm Immobilizing Agents; D014622:Vaginal Creams, Foams, and Jellies; C006912:carboxypolymethylene
keywords: Ammonium sulfate (PubChem CID: 6097028); Carbopol; Carbopol (PubChem CID: 6581); Contraception; Controlled release; Diethylaminoethyl-cellulose (PubChem CID: 16211032); Disodium hydrogen phosphate (PubChem CID: 24203); Sodium chloride (PubChem CID: 5234); Sodium dihydrogen orthophosphate (PubChem CID: 23672064); Sperm immobilization; Staphylococcus aureus