title: Molecular "light switch" [Ru(phen)2dppzidzo](2+) monitoring the aggregation of tau.
abstract: Monitoring the aggregation of the tau protein is a key protocol for elucidating the pathogenic mechanism of Alzheimer's disease. In the present article, [Ru(phen)2dppzidzo](2+), a "light switch" ruthenium(ii) complex, was presented as a new monitoring probe for the aggregation of a tau R3 peptide, the third repeat unit of the tau microtubule-binding domain. Having little impact on the aggregation process, large fixed Stokes shift and small background luminescence made the complex a better probe for monitoring the aggregation process and quantitatively detecting tau filaments compared to thioflavin S, a commonly used fluorescent dye for staining neurofibrillary tangles and monitoring tau aggregation. Furthermore, a long luminescence lifetime of this complex could also expand its potential usage in the detection of tau filaments in the presence of short-lived fluorescent backgrounds.
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Xing
authors.initials: X
authors.lastname: Gao
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Huang
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Jun-Liang
authors.initials: JL
authors.lastname: Yao
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Shi
authors.affiliation: Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China. wangli@tongji.edu.cn tmyao@tongji.edu.cn.
authors.forename: Tian-Ming
authors.initials: TM
authors.lastname: Yao
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D052160:Benzothiazoles; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008027:Light; D049408:Luminescent Agents / Q000737:chemistry* / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D066329:Protein Aggregates; D066263:Protein Aggregation, Pathological / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism; D016875:tau Proteins / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D049408:Luminescent Agents; D009942:Organometallic Compounds; D066329:Protein Aggregates; C466600:Ru(phen)(2)dipyrido(3,2-a-2',3'-c)phenazine; D013844:Thiazoles; D016875:tau Proteins; C009462:thioflavin T