title: Establishment of monoclonal HCC cell lines with organ site-specific tropisms.
abstract: BACKGROUND
  Organ site-specific metastasis is an ominous feature for most poor-prognostic hepatocellular carcinoma (HCC) patients. Cancer cell lines and animal models are indispensable for investigating the molecular mechanisms of organ specific tropism. However, till now, little is known about the drivers in HCC metastatic tropism, and also no effective way has been developed to block the process of tropistic metastasis.
  
  
  METHODS
  In this study, we established several monoclonal HCC cell lines from HCCLM3-RFP together with their xenograft models, and then analyzed their metastatic potentials and tropisms using in-vitro and in-vivo assays, and finally elucidated the driving forces of HCC tropistic metastases.
  
  
  RESULTS
  Six monoclonal cell lines with different organ site-specific tropism were established successfully. SPARC, VCAM1 and ANGPTL4 were found positively correlated with the potentials of lung metastasis, while ITGA1 had a positive relation to lymph node metastasis of enterocoelia.
  
  
  CONCLUSIONS
  By our powerful platforms, HCC metastatic tropisms in clinic could be easily mimicked and recapitulated for exploring the bilateral interactions between tumor and its microenvironment, elucidating the drivers of HCC metastatic tropisms, and testing anti-cancer effects of newly developed agent in pre-clinical stage.
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. wanjinliang01@163.com.|Department of Oncology, Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, 256603, People's Republic of China. wanjinliang01@163.com.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Wan
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. wenduo@outlook.com.
authors.forename: Duo
authors.initials: D
authors.lastname: Wen
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. dong2044@sohu.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Dong
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. judytangjun@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. loveliudongli@163.com.
authors.forename: Dongli
authors.initials: D
authors.lastname: Liu
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. lizzy0714@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. drtaozhh@126.com.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. gao.dongmei@zs-hospital.sh.cn.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Gao
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. sun.huichuan@zs-hospital.sh.cn.
authors.forename: Huichuan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Cancer Research Institute, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Central South University, Changsha, Hunan, China. ycao98@vip.sina.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. jiafan99@yahoo.com.|Institute of Biomedical Sciences of Fudan University, Shanghai, 200032, China. jiafan99@yahoo.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.affiliation: Liver Cancer Institute and Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, 200032, China. wu.weizhong@zs-hospital.sh.cn.|Liver Cancer Institute and Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. wu.weizhong@zs-hospital.sh.cn.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Wu
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002999:Clone Cells; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D064593:Heterografts; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis