title: Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination.
abstract: Particulate material is more efficient in eliciting immune responses. Here we describe the production of microspheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes by means of IC-Tagging, for their use as vaccines.
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Barreiro-Piñeiro
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Menaya-Vargas
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.|Department of Biochemistry, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
authors.forename: Alberto
authors.initials: A
authors.lastname: Brandariz-Núñez
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
authors.forename: Iria
authors.initials: I
authors.lastname: Otero-Romero
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Lostalé-Seijo
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Benavente
authors.affiliation: Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain. jose.martinez.costas@usc.es.
authors.forename: José M
authors.initials: JM
authors.lastname: Martínez-Costas
mesh_terms: D000818:Animals; D001717:Birds / Q000276:immunology / Q000821:virology; D000939:Epitopes / Q000276:immunology*; D005453:Fluorescence; D008863:Microspheres; D030101:Orthoreovirus, Avian / Q000187:drug effects* / Q000472:pathogenicity; D014611:Vaccination / Q000379:methods*; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000939:Epitopes; D017361:Viral Nonstructural Proteins
keywords: Adjuvants; Avian reovirus; IC-Tagging; Microspheres; Particulate material; Vaccines