title: Comparing the Primary and Recall Immune Response Induced by a New EV71 Vaccine Using Systems Biology Approaches.
abstract: Three inactivated EV71 whole-virus vaccines have completed Phase III clinical trials in mainland China, with high efficacy, satisfactory safety, and sustained immunogenicity. However, the molecular mechanisms how this new vaccine elicit potent immune response remain poorly understood. To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. The results showed that primary and recall response to EV71 antigen have both activated an IRF7 regulating type I interferon and antiviral immune response network. However, up-regulated genes involved in T cell activation regulated by IRF1, inflammatory response, B-cell activation and humoral immune response were only observed in recall response. The specific secretion of IL-10 in primary response and IL-2,IP-10,CCL14a, CCL21 in recall response was consistent with the activation of immune response process found in genes. Furthermore, the expression of MX1 and secretion of IP-10 in recall response were strongly correlated with NTAb level at 180d after vaccination (r = 0.81 and 0.99). In summary, inflammatory response, adaptive immune response and a stronger antiviral response were indentified in recall response.
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, P.R.China.
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Qunying
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Pan
authors.initials: P
authors.lastname: Chen
authors.affiliation: Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu, P.R.China.
authors.forename: Fengcai
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Miao
authors.initials: M
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Jilin University, Changchun, Jilin, P.R.China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Zhenglun
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Division of Hepatitis Virus Vaccines, National Institutes for Food and Drug Control, Beijing, P.R.China.
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002675:Child, Preschool; D017326:Clinical Trials, Phase III as Topic; D016000:Cluster Analysis; D016207:Cytokines / Q000378:metabolism; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007109:Immunity / Q000235:genetics; D007156:Immunologic Memory / Q000235:genetics; D007223:Infant; D016032:Randomized Controlled Trials as Topic; D015203:Reproducibility of Results; D049490:Systems Biology / Q000379:methods; D059467:Transcriptome; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D016207:Cytokines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines