title: Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on PLGA/Chitosan Electrospun Nano-Biocomposite.
abstract: OBJECTIVE
  In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated.
  
  
  METHODS
  In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and prolif- eration rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively.
  
  
  RESULTS
  H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05).
  
  
  CONCLUSIONS
  We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering.
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Razavi
authors.affiliation: Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Karbasi
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Morshed
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Zarkesh Esfahani
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Golozar
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Vaezifar
keywords: Chitosan; Mesenchymal Stem Cell; PLGA; Scaffold