title: Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
abstract: A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography. PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid. Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid. Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units. The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: He
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: An-Qiang
authors.initials: AQ
authors.lastname: Zhang
authors.affiliation: Hangzhou Baishanzu Biotech Co., Ltd., Hangzhou 310052, China.
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: Wang
authors.affiliation: Hangzhou Baishanzu Biotech Co., Ltd., Hangzhou 310052, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qu
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Guang-Lin
authors.initials: GL
authors.lastname: Li
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Li
authors.affiliation: Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address: sun_pl@zjut.edu.cn.
authors.forename: Pei-Long
authors.initials: PL
authors.lastname: Sun
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002849:Chromatography, Gas; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005947:Glucose; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D055492:Polyporus / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D005947:Glucose
keywords: Antioxidant activity; Polyporus umbellatus; Polysaccharide; Structure elucidation