title: Protein and cholesterol electrophoresis of plasma samples from captive cownose ray (Rhinoptera bonasus).
abstract: Our study was undertaken to assess the application of semiautomated methods available at the reference laboratory level for the evaluation of plasma protein and cholesterol via electrophoresis in samples from cownose rays (Rhinoptera bonasus). Three groups of animals were assessed: clinically normal, clinically abnormal, and parasitized with leeches. As reported previously, the albumin band was negligible; the protein electrophoretograms were dominated by a large beta-globulin fraction. While the group of samples from the leech-parasitized rays did not show any large differences, the abnormal group exhibited significantly elevated total solids and cholesterol levels. The latter was related to a significant increase in very low density lipoprotein levels. The results demonstrate the potential application of these laboratory methods in quantitation of plasma proteins and cholesterol fractions in subclass Elasmobranchii.
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field) ccray@med.miami.edu.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Cray
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Marilyn
authors.initials: M
authors.lastname: Rodriguez
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Cara
authors.initials: C
authors.lastname: Field
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Alexa
authors.initials: A
authors.lastname: McDermott
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Lynda
authors.initials: L
authors.lastname: Leppert
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Tonya
authors.initials: T
authors.lastname: Clauss
authors.affiliation: Division of Comparative Pathology, Department of Pathology and Laboratory Medicine, University of Miami Miller School of Medicine, Miami, FL (Cray, Rodriquez, Bossart)Georgia Aquarium, Atlanta, GA (McDermott, Leppert, Clauss, Bossart)The Marine Mammal Center, Sausolito, CA (Field).
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Bossart
mesh_terms: D000818:Animals; D001797:Blood Protein Electrophoresis / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D001798:Blood Proteins / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood*; D018940:Elasmobranchii / Q000097:blood*; D005260:Female; D008297:Male; D012016:Reference Values
chemical_list: D001798:Blood Proteins; D002784:Cholesterol
keywords: Cholesterol; elasmobranch; electrophoresis; lipoprotein; rays