title: Immobilized laccase-based biosensor for the detection of disubstituted methyl and methoxy phenols - application of Box-Behnken design with response surface methodology for modeling and optimization of performance parameters.
abstract: An amperometric principle-based biosensor, employing immobilized laccase enzyme from Trametes versicolor, was developed for the detection of disubstituted methyl and methoxy phenols. Three immobilization methods such as entrapment, cross-linking, and co-cross-linking, with bovine serum albumin (BSA) on nylon membrane have been compared. Among tested methods of immobilization, co-cross-linking method with BSA was superior to the other methods in terms of; sensitivity, limit of detection, response time, and operating stability. The increased sensitivity of the probe optimization of concentrations of laccase, BSA and glutaraldehyde can be achieved by, employing the Box-Behnken design of experiment.
authors.affiliation: a Department of Biotechnology Engineering , CMR Institute of Technology , Bangalore , India.|b Department of Chemistry , CMR Institute of Technology , Bangalore , India.
authors.forename: C
authors.initials: C
authors.lastname: Sarika
authors.affiliation: a Department of Biotechnology Engineering , CMR Institute of Technology , Bangalore , India.
authors.forename: K
authors.initials: K
authors.lastname: Rekha
authors.affiliation: b Department of Chemistry , CMR Institute of Technology , Bangalore , India.
authors.forename: B
authors.initials: B
authors.lastname: Narasimha Murthy
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D042845:Laccase / Q000737:chemistry*; D008956:Models, Chemical; D010636:Phenols / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D055454:Trametes / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D010636:Phenols; D012710:Serum Albumin, Bovine; D042845:Laccase
keywords: Amperometric; bovine serum albumin; co-cross-linking; nylon