title: Backbone tree for Chaetothyriales with four new species of Minimelanolocus from aquatic habitats.
abstract: We are studying the freshwater lignicolous fungi along a north-south latitudinal gradient in Asia. In this paper, fresh collections of Minimelanolocus from submerged wood in streams in Yunnan Province, China are characterised based on morphology and molecular phylogeny based on three rDNA regions: 18S (SSU), ITS1-5.8S-ITS2 (ITS) and 28S nuclear rDNA (LSU). The phylogenetic analysis of combined LSU and SSU sequence data and a separate analysis of ITS placed the isolates within the family Herpotrichiellaceae, order Chaetothyriales. An updated phylogenetic backbone tree for Chaetothyriales is provided with available ex-type and additional isolates. One of the isolates collected was identified as Minimelanolocus obscurus based on morphology and molecular data. Minimelanolocus aquaticus, M. asiaticus, M. curvatus and M. melanicus are described as new species considering the interspecific ITS variability and morphology. The phylogenetic placement of Minimelanolocus in Chaetothyriales is novel and provides new sequence data for the genus as a distinct lineage in Chaetothyriales. The conidial characters of all the known species in the genus are summarized. Descriptions and illustrations are provided for the five species of Minimelanolocus with notes on their taxonomy and phylogeny.
authors.affiliation: College of Basic Medicine, Dali University, Dali, Yunnan 671000, China; College of Agriculture and Biology, Dali University, Dali, Yunnan 671003, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
authors.forename: Dhanushka
authors.initials: D
authors.lastname: Udayanga
authors.affiliation: College of Basic Medicine, Dali University, Dali, Yunnan 671000, China; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
authors.forename: Zong-Long
authors.initials: ZL
authors.lastname: Luo
authors.affiliation: Institute of Biotechnology and Gerplamic Resources, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China.
authors.forename: Li-Jiao
authors.initials: LJ
authors.lastname: Chen
authors.affiliation: Faculty of Environmental Sciences & Engineering, Kunming University of Science & Technology, Kunming, Yunnan 650500, China.
authors.forename: De-Qun
authors.initials: DQ
authors.lastname: Zhou
authors.affiliation: College of Basic Medicine, Dali University, Dali, Yunnan 671000, China; Faculty of Environmental Sciences & Engineering, Kunming University of Science & Technology, Kunming, Yunnan 650500, China. Electronic address: suhongyan16@163.com.
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Su
authors.affiliation: Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Hyde
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D002681:China; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D005618:Fresh Water / Q000382:microbiology*; D014644:Genetic Variation; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S
keywords: Aquatic fungi; Black yeasts; Eurotiomycetes; Molecular phylogeny; Morphology