title: A Novel Pathogenesis-Related Class 10 Protein Gly m 4l, Increases Resistance upon Phytophthora sojae Infection in Soybean (Glycine max [L.] Merr.).
abstract: Phytophthora root and stem rot of soybean, caused by Phytophthora sojae (P. sojae), is a destructive disease in many soybean planting regions worldwide. In a previous study, an expressed sequence tag (EST) homolog of the major allergen Pru ar 1 in apricot (Prunus armeniaca) was identified up-regulated in the highly resistant soybean 'Suinong 10' infected with P. sojae. Here, the full length of the EST was isolated using rapid amplification of cDNA ends (RACE). It showed the highest homology of 53.46% with Gly m 4 after comparison with the eight soybean allergen families reported and was named Gly m 4-like (Gly m 4l, GenBank accession no. HQ913577.1). The cDNA full length of Gly m 4l was 707 bp containing a 474 bp open reading frame encoding a polypeptide of 157 amino acids. Sequence analysis suggests that Gly m 4l contains a conserved 'P-loop' (phosphate-binding loop) motif at residues 47-55 aa and a Bet v 1 domain at residues 87-120 aa. The transcript abundance of Gly m 4l was significantly induced by P. sojae, salicylic acid (SA), NaCl, and also responded to methyl jasmonic acid (MeJA) and ethylene (ET). The recombinant Gly m 4l protein showed RNase activity and displayed directly antimicrobial activity that inhibited hyphal growth and reduced zoospore release in P. sojae. Further analyses showed that the RNase activity of the recombinant protein to degrading tRNA was significantly affected in the presence of zeatin. Over-expression of Gly m 4l in susceptible 'Dongnong 50' soybean showed enhanced resistance to P. sojae. These results indicated that Gly m 4l protein played an important role in the defense of soybean against P. sojae infection.
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Sujie
authors.initials: S
authors.lastname: Fan
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Liangyu
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Collaborative Innovation Center of Grain Production Capacity Improvement in Heilongjiang Province, Harbin, 150086, Heilongjiang, People's Republic of China.
authors.forename: Junjiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Dong
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Xu
authors.affiliation: Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, 150030, Heilongjiang, People's Republic of China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Zhang
mesh_terms: D052179:Antigens, Plant / Q000096:biosynthesis*; D001483:Base Sequence; D060467:Disease Resistance; D008969:Molecular Sequence Data; D010838:Phytophthora; D010935:Plant Diseases / Q000382:microbiology*; D030262:Soybean Proteins / Q000096:biosynthesis*; D013025:Glycine max / Q000378:metabolism / Q000382:microbiology
chemical_list: D052179:Antigens, Plant; C530536:Gly m 4 allergen, Glycine max; D030262:Soybean Proteins