title: CXXC5 plays a role as a transcription activator for myelin genes on oligodendrocyte differentiation.
abstract: Myelination in corpus callosum plays important role for normal brain functions by transferring neurological information between various brain regions. However, the factors controlling expression of myelin genes in myelination are poorly understood. Here, CXXC5, a recently identified protein with CXXC-type zinc finger DNA binding motif, was characterized as a transcriptional activator of major myelin genes. We identified expression of CXXC5 expression was increased by Wnt/β-catenin signaling. CXXC5 specifically expressed in the white matter induced expression of myelin genes through the direct binding of CXXC DNA-binding motif of CXXC5 on the MBP promoter. During the differentiation of neural stem cells (NSCs) of CXXC5(-/-) mice, the expressions of myelin genes were simultaneously reduced. The CXXC5(-/-) mice exhibited severely reduction of myelin genes expression in corpus callosum as well as abnormalities in myelin structure. The disrupted structural integrity of myelin in the CXXC5(-/-) mice resulted in reduced electrical conduction amplitudes at corpus callosum. These findings indicate that the regulation of myelin genes expression by CXXC5 is important for forming myelin structure involved with axonal electrical signal transfer in the corpus callosum.
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Hyun-Yi
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Korea.
authors.forename: Jiso
authors.initials: J
authors.lastname: Hong
authors.affiliation: Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Korea.
authors.forename: Daesoo
authors.initials: D
authors.lastname: Kim
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Hyojung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Cheong
authors.affiliation: Department of Integrated OMICS For Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Yangsin
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Integrated OMICS For Biomedical Science, WCU Program of Graduate School, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Roth
authors.affiliation: Department of Pharmacology, Brain Research Institute, Brain Korea 21 Project for Medical Science, Severance Biomedical Science Institute, Yonsei University, College of Medicine, Seoul, 120-749, Korea.
authors.forename: Dong Goo
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, 609-735, Korea.
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.affiliation: Translational Research Center for Protein Function Control, Yonsei University, Seoul, 120-749, Korea.|Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea.
authors.forename: Kang-Yell
authors.initials: KY
authors.lastname: Choi
mesh_terms: D000200:Action Potentials / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D003337:Corpus Callosum / Q000254:growth & development / Q000378:metabolism; D004268:DNA-Binding Proteins; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation / Q000235:genetics*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism*; D009431:Neural Conduction / Q000235:genetics; D058953:Neural Stem Cells; D009836:Oligodendroglia / Q000502:physiology* / Q000648:ultrastructure; D014157:Transcription Factors; D060449:Wnt Signaling Pathway / Q000235:genetics; D060509:Wnt3A Protein / Q000494:pharmacology; D051176:beta Catenin / Q000378:metabolism
chemical_list: C000601091:CXXC5 protein, mouse; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D047908:Intracellular Signaling Peptides and Proteins; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein; C047516:Plp1 protein, mouse; D014157:Transcription Factors; D060509:Wnt3A Protein; C555511:Wnt3a protein, mouse; D051176:beta Catenin
keywords: CXXC5; Wnt/β-catenin signaling; neural stem cell; oligodendrocyte; transcription factor