title: Simultaneous separation of chlorinated/brominated dioxins, polychlorinated biphenyls, polybrominated diphenyl ethers and their methoxylated derivatives from hydroxylated analogues on molecularly imprinted polymers prior to gas/liquid chromatography and mass spectrometry.
abstract: Polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are important contaminants in the environment. In recent years also polybrominated dibenzo-p-dioxins/dibenzofurans (PBDD/Fs) have been reported as emerging environmental stressors. It has been suggested that hydroxylated polybrominated biphenyl ethers (OH-BDEs) are important - may be even the most important - precursors of brominated dioxins/furans. The aim of this study was to develop a robust, time-saving analytical procedure to collectively separate in animal tissues such compounds prior to determination of individual substances with a gas/liquid chromatograph coupled to an ion trap/time-of-flight mass spectrometer (GC/IT-MS or UPLC/TOF-MS). Two OH-CB/BDE separation methods including alkaline extraction and molecularly imprinted polymers (MIP) were compared. Performance of both methods was validated: absolute recovery values were in the 47-90% range depending on the compound, while recovery relative standard deviation was below 20% in most cases. Levels of the studied compounds have been assessed in some real life samples.
authors.affiliation: Department of Food Analysis, Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, Poland. Electronic address: roszko@ibprs.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Roszko
authors.affiliation: Department of Food Analysis, Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, Poland. Electronic address: szymczyk@ibprs.ple.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Szymczyk
authors.affiliation: Department of Food Analysis, Institute of Agricultural and Food Biotechnology, Rakowiecka 36, 02-532 Warsaw, Poland. Electronic address: jedrzejczak@ibprs.pl.
authors.forename: Renata
authors.initials: R
authors.lastname: Jędrzejczak
mesh_terms: D000818:Animals; D002645:Chickens; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D004147:Dioxins / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D048389:Gadus morhua; D055768:Halogenated Diphenyl Ethers / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D054879:Halogenation; D006900:Hydroxylation; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry; D054802:Molecular Imprinting; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000097:blood / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D004147:Dioxins; D055768:Halogenated Diphenyl Ethers; D011078:Polychlorinated Biphenyls
keywords: Brominated dioxins; Fractionation; Hydroxylated polibrominated diphenyl ethers; Molecularly imprinted polymers