title: [IMMUNE REGULATORY PROPERTIES OF BIFIDOBACTERIA METABOLITES DURING EUBIOSIS AND DYSBIOSIS OF THE HUMAN COLON].
abstract: OBJECTIVE
  Evaluate immune regulatory properties of bifidobacteria metabolites during eubiosis and dysbiosis of the human colon.
  
  
  METHODS
  Anti-cytokine activity of metabolites of bifidobacteria clinical strains and their ability to influence the production of pro- and anti-inflammatory cytokines (IL-10) by peripheral blood mononuclear cells of healthy humans was studied, taking into account microecological state of the human intestine. Determination of final concentration of cytokines in experimental and control samples was carried out by EIA.
  
  
  RESULTS
  Sensitive parameters, that are suitable for evaluation of stability of human intestine microsymbiocenosis, were detected. The level of microbial seeding, concentration of TNF-α and anti-lysozyme activity turned out to be informative for bifidobacteria in eubiosis conditions. The ability of bifidoflora metabolites to influence the production of pro-inflammatory cytokines (INF-γ, TNF-α, IL-8) by human mononuclears was a significant parameter during formation of 1 - 3 degree dysbiosis.
  
  
  CONCLUSIONS
  The maintenance of physiological state of intestine homeostasis is determined by immune regulatory properties of bifidobacteria metabolites, that is realized via their interaction with both cytokines (anti-cytokine activity) and production of cytokines by host immune cells (peripheral blood mononuclears).
authors.forename: O V
authors.initials: OV
authors.lastname: Bukharin
authors.forename: E V
authors.initials: EV
authors.lastname: Ivanova
authors.forename: N B
authors.initials: NB
authors.lastname: Perunova
authors.forename: I N
authors.initials: IN
authors.lastname: Chainikova
authors.forename: I A
authors.initials: IA
authors.lastname: Nikoforov
authors.forename: T A
authors.initials: TA
authors.lastname: Bondarenko
mesh_terms: D000293:Adolescent; D000328:Adult; D001644:Bifidobacterium / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003106:Colon / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D064806:Dysbiosis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D016207:Cytokines