title: Long non-coding RNA HOTAIR regulates cyclin J via inhibition of microRNA-205 expression in bladder cancer.
abstract: The level of microRNA-205 (miR-205) is commonly deregulated in a number of cancers. Through the screening of the microRNA expression profile in bladder cancer tissue and cell lines, we found that expression of miR-205 was significantly suppressed. In addition, the levels of miR-205 expression had a negative correlation with the degree of bladder cancer malignancy. However, the biological functions of miR-205 remained unclear. In this study, we have demonstrated that miR-205 had a role in the inhibition of proliferation, migration and invasion of bladder cancer cells. Moreover, we have identified cyclin J (CCNJ) gene, which is involved in cell cycle regulation, as a novel target for miR-205. Furthermore, a long non-coding RNA HOTAIR (HOX transcript antisense RNA) was observed to participate in the silencing of miR-205 in bladder cancer cells by breaking the balance of histone modification between H3K4me3 (histone H3 at lysine 4 methylation) and H3K27me3 on miR-205 promoter. This study elucidates an important role that miR-205 had in the regulation of proliferation, migration and invasion of bladder cancer cells, suggesting a potential therapeutic target for combating bladder cancer.
authors.affiliation: Department of Immunology, School of Basic Medical Science, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Immunology, School of Basic Medical Science, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: P
authors.initials: P
authors.lastname: Du
authors.affiliation: Department of Immunology, School of Basic Medical Science, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: W
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Experimental Teaching Center, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Du
authors.affiliation: Experimental Teaching Center, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Immunology, School of Basic Medical Science, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Immunology, School of Basic Medical Science, Binzhou Medical University, Yantai, Shandong, China.
authors.forename: T
authors.initials: T
authors.lastname: Hu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D002465:Cell Movement; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D062085:RNA, Long Noncoding / Q000502:physiology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D016213:Cyclins; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000589891:CCNJ protein, human; C561566:HOTAIR long untranslated RNA, human; C534425:MIR205, human