title: Biomimetic chimeric peptide-tethered hydrogels for human mesenchymal stem cell delivery.
abstract: Here, we report a chimeric peptide-tethered fibrin hydrogel scaffold for delivery of human mesenchymal stem cells (hMSC). Osteopontin-derived peptide (OP) was used as an hMSC-tethering moiety. OP showed hMSC adhesion properties and enhanced hMSC proliferation. A natural fibrin-binding protein-derived peptide (FBP) was tested for its ability to tether hMSC to the fibrin gel matrix. FBP loading on fibrin gels was 8.2-fold higher than that of a scrambled peptide (scFBP). FBP-loaded fibrin gels were retained at injection sites longer than scFBP-loaded fibrin gels, showing a 15.9-fold higher photon intensity of fluorescent FBP-grafted fibrin gels than fluorescent scFBP-loaded fibrin gels 48 h after injection. On the basis of the fibrin gel-binding properties of FBP and the hMSC-binding and proliferation-supporting properties of OP, we constructed chimeric peptides containing FBP and OP linked with a spacer (FBPsOP). Four days after transplantation, the survival of hMSC in FBPsOP-grafted fibrin gels was 3.9-fold higher than hMSC in fibrin gels alone. Our results suggest the potential of FBPsOP-grafted fibrin gels as a bioactive delivery system for enhanced survival of stem cells.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Daehak-dong, Gwanank-gu, Seoul 151-742, Republic of Korea.
authors.forename: Gayong
authors.initials: G
authors.lastname: Shim
authors.affiliation: Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science, Seoul National University, Daehak-dong, Gwanank-gu, Seoul, Republic of Korea.
authors.forename: Gunwoo
authors.initials: G
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Daehak-dong, Gwanank-gu, Seoul 151-742, Republic of Korea.
authors.forename: Junhyeok
authors.initials: J
authors.lastname: Choi
authors.affiliation: Translational Research Center and Inha University School of Medicine, Incheon, Republic of Korea; Inha Research Institute for Medical Sciences, Inha University School of Medicine, Incheon, Republic of Korea; Homeotherapy Co. Ltd., Incheon, Republic of Korea.
authors.forename: TacGhee
authors.initials: T
authors.lastname: Yi
authors.affiliation: Homeotherapy Co. Ltd., Incheon, Republic of Korea.
authors.forename: Yun Kyoung
authors.initials: YK
authors.lastname: Cho
authors.affiliation: Translational Research Center and Inha University School of Medicine, Incheon, Republic of Korea; Homeotherapy Co. Ltd., Incheon, Republic of Korea.
authors.forename: Sun Uk
authors.initials: SU
authors.lastname: Song
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Daehak-dong, Gwanank-gu, Seoul 151-742, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science, Seoul National University, Daehak-dong, Gwanank-gu, Seoul, Republic of Korea.
authors.forename: Youngro
authors.initials: Y
authors.lastname: Byun
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Daehak-dong, Gwanank-gu, Seoul 151-742, Republic of Korea. Electronic address: ohyk@snu.ac.kr.
authors.forename: Yu-Kyoung
authors.initials: YK
authors.lastname: Oh
mesh_terms: D000818:Animals; D032701:Biomimetics; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000008:administration & dosage*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010455:Peptides / Q000008:administration & dosage*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage
chemical_list: D020100:Hydrogels; D010455:Peptides; D011993:Recombinant Fusion Proteins
keywords: Biomimetic chimeric peptide; Cell delivery; Fibrin-binding protein-derived peptide; Human mesenchymal stem cells; Osteopontin-derived peptide