title: Pin1 facilitates NF-κB activation and promotes tumour progression in human hepatocellular carcinoma.
abstract: BACKGROUND
  NF-κB promotes HCC progression; however, therapies targeting NF-κB are not used due to severe adverse reactions. Pin1 is reported to induce tumour progression in vitro. However, the role of Pin1 in HCC is unclear. Moreover, little is known about the mechanism of Pin1-mediated NF-κB activation.
  
  
  METHODS
  Fresh surgical specimens were collected from 144 HCC patients. Pin1 and NF-κB-p65 expression was evaluated by immunohistochemistry and western blotting. NF-κB activation was assessed by EMSA.
  
  
  RESULTS
  Pin1 was increased in HCC compared to adjacent liver tissue. The multivariate analysis revealed that high Pin1 expression was an independent factor for poor prognosis. In HCC with high Pin1 expression, tumour size was larger and portal vein invasion was increased. Pin1 expression was correlated with phosphorylated (p-) NF-κB-p65(Thr254) and p-NF-κB-p65(Ser276), and thereby NF-κB activation. Pin1-induced NF-κB activation accelerated cell cycle progression, induced angiogenesis, and inhibited apoptosis. Pin1 knockdown in HCC cells inhibited the phosphorylation of NF-κB-p65(Ser276), and reduced NF-κB activation, which resulted in inhibiting tumour cell progression. When HCC cells were treated with the Pin1 inhibitors, p-NF-κB-p65(Ser276) expression and NF-κB activation was reduced, and cell proliferation was inhibited.
  
  
  CONCLUSIONS
  Pin1 is associated with aggressive tumour progression and poor prognosis in HCC by mediating NF-κB activation.
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Kimio
authors.initials: K
authors.lastname: Shinoda
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kuboki
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimizu
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohtsuka
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshitomi
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furukawa
authors.affiliation: Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba 260-0856, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D016328:NF-kappa B / Q000235:genetics*; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D019696:Peptidylprolyl Isomerase / Q000235:genetics*; D010766:Phosphorylation / Q000235:genetics; D011379:Prognosis; D051996:Transcription Factor RelA / Q000235:genetics
chemical_list: D016328:NF-kappa B; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D051996:Transcription Factor RelA; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase