title: Peritoneal Dialysis Catheter Increases Leukocyte Recruitment in the Mouse Parietal Peritoneum Microcirculation and Causes Fibrosis.
abstract: ♦
  
  
  BACKGROUND
  The objective of this study was to examine the effects of a conventional dialysis solution and peritoneal catheter on leukocyte-endothelial cell interactions in the microcirculation of the parietal peritoneum in a subacute peritoneal dialysis (PD) mouse model. ♦
  
  
  METHODS
  An intraperitoneal (IP) catheter with a subcutaneous injection port was implanted into mice and, after a 2-week healing period, the animals were injected daily for 6 weeks with a 2.5% dextrose solution. Intravital microscopy (IVM) of the parietal peritoneum microcirculation was performed 4 hours after the last injection of the dialysis solution. Leukocyte-endothelial cell interactions were quantified and compared with catheterized controls without dialysis treatment and naïve mice. ♦
  
  
  RESULTS
  The number of rolling and extravascular leukocytes along with peritoneal fibrosis and neovascularization were significantly increased in the catheterized animals compared with naïve mice but did not significantly differ between the 2 groups of catheterized animals with sham injections or dialysis solution treatment. ♦
  
  
  CONCLUSIONS
  The peritoneal catheter implant increased leukocyte rolling and extravasation, peritoneal fibrosis and vascularization in the parietal peritoneum independently from the dialysis solution treatment.
authors.affiliation: Medical Sciences Graduate Program, McMaster University, Hamilton, ON, Canada.
authors.forename: Paulina M
authors.initials: PM
authors.lastname: Kowalewska
authors.affiliation: Division of Nephrology, St. Joseph's Hospital Department of Medicine, McMaster University, Hamilton, ON, Canada.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Margetts
authors.affiliation: Thrombosis and Atherosclerosis Research Institute and the Department of Medicine, McMaster University, Hamilton, ON, Canada afoxrob@mcmaster.ca.
authors.forename: Alison E
authors.initials: AE
authors.lastname: Fox-Robichaud
mesh_terms: D000818:Animals; D002408:Catheters, Indwelling / Q000009:adverse effects*; D002634:Chemotaxis, Leukocyte; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation; D010530:Peritoneal Dialysis / Q000295:instrumentation*; D056627:Peritoneal Fibrosis / Q000276:immunology*; D010537:Peritoneum / Q000098:blood supply / Q000276:immunology*
keywords: Intravital microscopy; angiogenesis; collagen; elastic fibers; endothelium; foreign body; inflammation; mesothelium