title: Development of a rapid method for the quantification of cellulose in tobacco by (13)C CP/MAS NMR.
abstract: A method was developed for rapid quantitative determination of cellulose in tobacco by utilizing (13)C cross polarization magic angle spinning NMR spectroscopy ((13)C CP/MAS NMR). Sample powder was loaded into NMR rotor, which was customized rotor containing a matched silicon tube as an intensity reference. (13)C CP/MAS NMR spectra of tobacco samples were processed with spectral deconvolution to obtain the area of the C-1 resonance at 105.5ppm and the internal standard at 0ppm. The ratio between the area of 105.5ppm and 0ppm of a set of standard cellulose samples was used to construct a calibration curve. The cellulose content of a tobacco sample was determined by comparison of the ratio between the area of 105.5ppm and 0ppm to the calibration curve. Results of this developed method showed good agreement with those obtained from chemical analysis. The proposed method has such advantages of accuracy, quickness and efficiency, and could be an alternative to chemical analyses of cellulose.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230026, People's Republic of China.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD, 606 Huangshan Road, Hefei 230088, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD, 606 Huangshan Road, Hefei 230088, People's Republic of China. Electronic address: tianzfeng@126.com.
authors.forename: Zhenfeng
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD, 606 Huangshan Road, Hefei 230088, People's Republic of China.
authors.forename: Kaibo
authors.initials: K
authors.lastname: Chen
authors.affiliation: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD, 606 Huangshan Road, Hefei 230088, People's Republic of China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Ge
authors.affiliation: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD, 606 Huangshan Road, Hefei 230088, People's Republic of China.
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230026, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Tian
authors.affiliation: Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei 230026, People's Republic of China. Electronic address: yjun8202@ustc.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D002482:Cellulose / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D002482:Cellulose
keywords: (13)C CP/MAS NMR; Cellulose; Spectral deconvolution; Tobacco