title: Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
abstract: During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced. In this study, the wastewater generated during the canning of mushroom was analyzed. From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography. Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively. The monosaccharide composition analysis using gas chromatography (GC) showed that the four fractions were heteropolysaccharides and mainly composed of glucose. Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages. Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied. Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration. These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Huang
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Ou
authors.affiliation: School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.
authors.forename: Tai Wai David
authors.initials: TW
authors.lastname: Yew
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Jingna
authors.initials: J
authors.lastname: Liu
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Leng
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Su
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Jiaofen
authors.initials: J
authors.lastname: Lin
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Li
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian 363000, People's Republic of China. Electronic address: xmpyt@sina.com.
authors.forename: Yutian
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000364:Agaricus / Q000737:chemistry* / Q000302:isolation & purification; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000009:adverse effects; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D062065:Wastewater / Q000382:microbiology
chemical_list: D000410:Alanine Transaminase; D001219:Aspartate Aminotransferases; D002251:Carbon Tetrachloride; D062610:Fungal Polysaccharides; D009005:Monosaccharides; D020011:Protective Agents; D062065:Wastewater
keywords: A. bisporus; Hepatoprotective activity; Industrial wastewater; Polysaccharide