title: Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells.
abstract: Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel material and device for the delivery of biomacromolecules into live cells. We attribute the successful results to the unique features of the system, which include high-aspect-ratio, uniform nanoneedles laid across a 2D array, combined with an oscillatory feature, which together allow rapid, forcible and efficient insertion and protein release into thousands of cells simultaneously.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsumoto
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Ramachandra
authors.initials: R
authors.lastname: Rao Sathuluri
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kato
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Yaron R
authors.initials: YR
authors.lastname: Silberberg
authors.affiliation: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Ryuzo
authors.initials: R
authors.lastname: Kawamura
authors.affiliation: Department of Mechanical Engineering, Shizuoka University, 3-5-1 Johoku, Hamamatsu, Shizuoka, 432-8561, Japan.
authors.forename: Futoshi
authors.initials: F
authors.lastname: Iwata
authors.affiliation: Research Center for Ubiquitous MEMS and Micro Engineering, AIST, 1-2-1 Namiki, Tsukuba, Ibaraki 305-8564, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.|Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central5 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Chikashi
authors.initials: C
authors.lastname: Nakamura
mesh_terms: D018014:Gene Transfer Techniques / Q000295:instrumentation*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D009339:Needles; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*; D046888:Tissue Array Analysis / Q000295:instrumentation
chemical_list: D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C045073:Cre recombinase; D019426:Integrases; D012825:Silicon