title: Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules.
abstract: The subdivision of cell populations in compartments is a key event during animal development. In Drosophila, the gene apterous (ap) divides the wing imaginal disc in dorsal vs ventral cell lineages and is required for wing formation. ap function as a dorsal selector gene has been extensively studied. However, the regulation of its expression during wing development is poorly understood. In this study, we analyzed ap transcriptional regulation at the endogenous locus and identified three cis-regulatory modules (CRMs) essential for wing development. Only when the three CRMs are combined, robust ap expression is obtained. In addition, we genetically and molecularly analyzed the trans-factors that regulate these CRMs. Our results propose a three-step mechanism for the cell lineage compartment expression of ap that includes initial activation, positive autoregulation and Trithorax-mediated maintenance through separable CRMs.
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Dimitri
authors.initials: D
authors.lastname: Bieli
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Oguz
authors.initials: O
authors.lastname: Kanca
authors.affiliation: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Requena
authors.affiliation: Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland.
authors.forename: Fisun
authors.initials: F
authors.lastname: Hamaratoglu
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.
authors.forename: Daryl
authors.initials: D
authors.lastname: Gohl
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.
authors.forename: Paul
authors.initials: P
authors.lastname: Schedl
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Markus
authors.initials: M
authors.lastname: Affolter
authors.affiliation: Department of Biomedical Sciences, University of Minnesota Medical School. Duluth, Minnesota, United States of America.
authors.forename: Matthew
authors.initials: M
authors.lastname: Slattery
authors.affiliation: Biozentrum, University of Basel, Basel, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Müller
authors.affiliation: Departamento de Biología Molecular and Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Estella
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D060227:Imaginal Discs / Q000254:growth & development; D060850:LIM-Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D029721:Drosophila Proteins; C063459:En protein, Drosophila; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; C074459:ap protein, Drosophila