title: Antioxidant Action of Apelin-12 Peptide and Its Structural Analog In Vitro.
abstract: The effects of C-terminal fragment of natural peptide apelin-12 H-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe-OH (A12) and its structural analog H-(N(α)Me)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH (AI) on Cu(2+)-induced free radical oxidation of low-density lipoprotein in human blood plasma and activity of commercially available enzymes superoxide dismutase and catalase in a concentration range of 0.01-1 mM were examined. A12 and AI had no effect on superoxide dismutase and catalase activities during 24-h co-incubation with these enzymes at 4°C. When used in a concentration of 1 mM, A12 and AI decreased the maximum low-density lipoprotein oxidation rate by 51 and 47%, respectively, and lengthened the lag phase of low-density lipoprotein oxidation by 2.6 and 1.8 times, respectively, which confirmed their antioxidant potency.
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. newera@yandex.ru.
authors.forename: Y A
authors.initials: YA
authors.lastname: Pelogeykina
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: G G
authors.initials: GG
authors.lastname: Konovalova
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Pisarenko
authors.affiliation: Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: V Z
authors.initials: VZ
authors.lastname: Lankin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry*; D002374:Catalase / Q000737:chemistry*; D002417:Cattle; D057075:Enzyme Assays; D004912:Erythrocytes / Q000737:chemistry / Q000201:enzymology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques; D012996:Solutions; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D036341:Intercellular Signaling Peptides and Proteins; D008077:Lipoproteins, LDL; D012996:Solutions; C495200:apelin-12 peptide; D002374:Catalase; D013482:Superoxide Dismutase
keywords: apelin-12; catalase; low-density lipoprotein free radical oxidation; myocardial ischemia/reperfusion; superoxide dismutase