title: MicroRNA panels as disease biomarkers distinguishing hepatitis B virus infection caused hepatitis and liver cirrhosis.
abstract: An important unresolved clinical issue is to distinguish hepatitis B virus (HBV) infection caused chronic hepatitis and their corresponding liver cirrhosis (LC). Recent research suggests that circulating microRNAs are useful biomarkers for a wide array of diseases. We analyzed microRNA profiles in the plasmas of a total of 495 chronic hepatitis B (CHB) patients, LC patients and healthy donors and identified 10 miRNAs that were differentially expressed between CHB and LC patients. Our logistic models show that three panels of miRNAs have promising diagnostic performances in discriminating CHB from LC. Blinded tests were subsequently conducted to evaluate the diagnostic performances in clinical practice and a sensitivity of 85% and specificity of 70% have been achieved in separating CHB from LC pateints. The expression levels of some circulating miRNAs were significantly correlated with HBV DNA load and liver function, such as prothrombin activity (PTA) and levels of alanin aminotransferase (ALT), albumin (ALB) and cholinesterase (CHE). Our results provide important information for developing novel diagnostic tools for distinguishing chronic HBV hepatitis and their corresponding cirrhosis.
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, No.8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
authors.forename: Bo-Xun
authors.initials: BX
authors.lastname: Jin
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, No.8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Beijing QuantoBio Biotechnology Co. Ltd, No.1, Disheng East Road, Beijing Economic-Technological Development Area, Daxing District, Beijing, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Jin
authors.affiliation: Beijing QuantoBio Biotechnology Co. Ltd, No.1, Disheng East Road, Beijing Economic-Technological Development Area, Daxing District, Beijing, China.
authors.forename: Xiang-Ying
authors.initials: XY
authors.lastname: Sun
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, No.8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Qiao
authors.affiliation: Beijing QuantoBio Biotechnology Co. Ltd, No.1, Disheng East Road, Beijing Economic-Technological Development Area, Daxing District, Beijing, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Wei
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, No.8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Sun
authors.affiliation: Beijing QuantoBio Biotechnology Co. Ltd, No.1, Disheng East Road, Beijing Economic-Technological Development Area, Daxing District, Beijing, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Zhang
authors.affiliation: Beijing YouAn Hospital, Capital Medical University, No.8, Xitoutiao, Youanmenwai, Fengtai District, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002802:Cholinesterases / Q000097:blood; D004279:DNA, Viral / Q000097:blood / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D020869:Gene Expression Profiling; D006515:Hepatitis B virus / Q000502:physiology*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D008111:Liver Function Tests; D016015:Logistic Models; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011516:Prothrombin / Q000378:metabolism; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000378:metabolism; D019562:Viral Load
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D035683:MicroRNAs; D012709:Serum Albumin; D011516:Prothrombin; D000410:Alanine Transaminase; D002802:Cholinesterases