title: Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats.
abstract: Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequate Se diet and groups of WKY HSe and SHR HSe that received Se supplementation. The Se nutritional status was assessed by measuring whole blood glutathione peroxidase-1 (GPx-1) activity. Serum concentration of lipid hydroperoxides and serum level of antibodies against advanced glycation end products (anti-AGEs abs) were determined. Expression of GPx-1 and endothelial nitric oxide synthase (eNOS) were examined in aortic wall. Se supplementation significantly increased GPx-1 activity of whole blood and in the aortas of WKY and SHR. Decreased lipid peroxidation level, eNOS-3 expression in the aortic wall, and serum level of anti-AGEs abs were found in SHR HSe compared with SHR NSe. In conclusion, Se supplementation improved the redox status of the aortic wall in young SHR.
authors.affiliation: Department of Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Boryana
authors.initials: B
authors.lastname: Ruseva
authors.affiliation: Department of Biology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Milena
authors.initials: M
authors.lastname: Atanasova
authors.affiliation: Department of Common and Clinical Pathology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Reni
authors.initials: R
authors.lastname: Tsvetkova
authors.affiliation: Department of Common and Clinical Pathology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Betova
authors.affiliation: Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 73 Tsarigradsko Shose, 1113 Sofia, Bulgaria.
authors.forename: Margarita
authors.initials: M
authors.lastname: Mollova
authors.affiliation: Department of Biophysics, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Margarita
authors.initials: M
authors.lastname: Alexandrova
authors.affiliation: Central Clinical Laboratory of University Hospital, 8 Georgi Kochev, 5800 Pleven, Bulgaria.
authors.forename: Pavlina
authors.initials: P
authors.lastname: Laleva
authors.affiliation: Department of Pathological Physiology, Medical University, 1 Kliment Ohridski, 5800 Pleven, Bulgaria.
authors.forename: Aneliya
authors.initials: A
authors.lastname: Dimitrova
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019587:Dietary Supplements; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005979:Glutathione Peroxidase / Q000097:blood; D017127:Glycation End Products, Advanced / Q000276:immunology; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012643:Selenium / Q000494:pharmacology*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000906:Antibodies; D017127:Glycation End Products, Advanced; D008054:Lipid Peroxides; D005979:Glutathione Peroxidase; D052250:Nitric Oxide Synthase Type III; D012643:Selenium; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat