title: Structure of the novel monomeric glyoxalase I from Zea mays.
abstract: The glyoxalase system is ubiquitous among all forms of life owing to its central role in relieving the cell from the accumulation of methylglyoxal, a toxic metabolic byproduct. In higher plants, this system is upregulated under diverse metabolic stress conditions, such as in the defence response to infection by pathogenic microorganisms. Despite their proven fundamental role in metabolic stresses, plant glyoxalases have been poorly studied. In this work, glyoxalase I from Zea mays has been characterized both biochemically and structurally, thus reporting the first atomic model of a glyoxalase I available from plants. The results indicate that this enzyme comprises a single polypeptide with two structurally similar domains, giving rise to two lateral concavities, one of which harbours a functional nickel(II)-binding active site. The putative function of the remaining cryptic active site remains to be determined.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Gino L
authors.initials: GL
authors.lastname: Turra
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Romina B
authors.initials: RB
authors.lastname: Agostini
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), CC 31, B2700KXC Pergamino, Argentina.
authors.forename: Carolina M
authors.initials: CM
authors.lastname: Fauguel
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria (INTA), CC 31, B2700KXC Pergamino, Argentina.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Presello
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Carlos S
authors.initials: CS
authors.lastname: Andreo
authors.affiliation: Protein Crystallography Station, Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
authors.forename: Javier M
authors.initials: JM
authors.lastname: González
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
authors.forename: Valeria A
authors.initials: VA
authors.lastname: Campos-Bermudez
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D007791:Lactoylglutathione Lyase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009532:Nickel / Q000378:metabolism; D011487:Protein Conformation; D016415:Sequence Alignment; D003313:Zea mays / Q000737:chemistry* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D009532:Nickel; D007791:Lactoylglutathione Lyase
keywords: glyoxalase; maize; methylglyoxal