title: Dynamics of histone H3 acetylation in the nucleosome core during mouse pre-implantation development.
abstract: In mammals, the time period that follows fertilization is characterized by extensive chromatin remodeling, which enables epigenetic reprogramming of the gametes. Major changes in chromatin structure persist until the time of implantation, when the embryo develops into a blastocyst, which comprises the inner cell mass and the trophectoderm. Changes in DNA methylation, histone variant incorporation, and covalent modifications of the histones tails have been intensively studied during pre-implantation development. However, modifications within the core of the nucleosomes have not been systematically analyzed. Here, we report the first characterization and temporal analysis of 3 key acetylated residues in the core of the histone H3: H3K64ac, H3K122ac, and H3K56ac, all located at structurally important positions close to the DNA. We found that all 3 acetylations occur during pre-implantation development, but with different temporal kinetics. Globally, H3K64ac and H3K56ac were detected throughout cleavage stages, while H3K122ac was only weakly detectable during this time. Our work contributes to the understanding of the contribution of histone modifications in the core of the nucleosome to the "marking" of the newly established embryonic chromatin and unveils new modification pathways potentially involved in epigenetic reprogramming.
authors.affiliation: a Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM U964 , Université de Strasbourg, Illkirch, Cité Universitaire de Strasbourg , France.
authors.forename: Céline
authors.initials: C
authors.lastname: Ziegler-Birling
authors.affiliation: a Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM U964 , Université de Strasbourg, Illkirch, Cité Universitaire de Strasbourg , France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Daujat
authors.affiliation: a Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM U964 , Université de Strasbourg, Illkirch, Cité Universitaire de Strasbourg , France.
authors.forename: Robert
authors.initials: R
authors.lastname: Schneider
authors.affiliation: a Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM U964 , Université de Strasbourg, Illkirch, Cité Universitaire de Strasbourg , France.
authors.forename: Maria-Elena
authors.initials: ME
authors.lastname: Torres-Padilla
mesh_terms: D000107:Acetylation; D000818:Animals; D042002:Chromatin Assembly and Disassembly; D010064:Embryo Implantation; D044127:Epigenesis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D042421:Histone Code; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
chemical_list: D006657:Histones; D009707:Nucleosomes
keywords: Epigenetics; histone acetylation; lateral surface; mammalian embryo; nucleosome core