title: Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells.
abstract: Peripheral blood can provide valuable information on an individual's immune status. Cell-based assays typically target leukocytes and their products. Characterization of leukocytes from whole blood requires their separation from the far more numerous red blood cells.1 Current methods to classify leukocytes, such as recovery on antibody-coated beads or fluorescence-activated cell sorting require long sample preparation times and relatively large sample volumes.2 A simple method that enables the characterization of cells from a small peripheral whole blood sample could overcome limitations of current analytical techniques. We describe the development of a simple graphene oxide surface coated with single-domain antibody fragments. This format allows quick and efficient capture of distinct WBC subpopulations from small samples (∼30 μL) of whole blood in a geometry that does not require any specialized equipment such as cell sorters or microfluidic devices.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA).|Present address: Institute of Biomedical Engineering, National Chiao Tung University, Hsinchu 30010 (Taiwan).
authors.forename: Guan-Yu
authors.initials: GY
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Zeyang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA).
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Theile
authors.affiliation: Department of Materials Science and Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Neelkanth M
authors.initials: NM
authors.lastname: Bardhan
authors.affiliation: Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Priyank V
authors.initials: PV
authors.lastname: Kumar
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA).
authors.forename: Joao N
authors.initials: JN
authors.lastname: Duarte
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA).
authors.forename: Takeshi
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142 (USA).
authors.forename: Ali
authors.initials: A
authors.lastname: Rashidfarrokh
authors.affiliation: Department of Materials Science and Engineering, Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
authors.forename: Angela M
authors.initials: AM
authors.lastname: Belcher
authors.affiliation: Department of Biology, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142 (USA). ploegh@wi.mit.edu.
authors.forename: Hidde L
authors.initials: HL
authors.lastname: Ploegh
mesh_terms: D006108:Graphite / Q000097:blood / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D061905:Single-Domain Antibodies / Q000097:blood / Q000276:immunology*
chemical_list: D061905:Single-Domain Antibodies; D006108:Graphite
keywords: blood; cell detection; graphene oxide; single domain antibody; sortase