title: DNA methylation and detection of cervical cancer and precancerous lesions using molecular methods.
abstract: Cervical cancer is the third most common cancer disease affecting the female population, and a key factor in the development of the disease is the human papillomavirus infection (HPV). The disease is also impacted by epigenetic changes such as DNA methylation, which causes activation or exclusion of certain genes. The aim of our review is to summarize and compare the most common molecular methods for detection of methylated promoter regions in biomarkers occurring in cervical carcinoma and also show the importance of connections of HR-HPV testing with methylation analysis in patients with cervical intraepithelial neoplasia. Insight into genetic and epigenetic alterations associated with cervical cancer development can offer opportunities for the molecular biomarkers that can be useful for screening, diagnosis, and also as new ways of treatment of cervical cancer precursor lesions.
authors.affiliation: Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4 A, Martin, 036 01, Slovakia.
authors.forename: Sandra
authors.initials: S
authors.lastname: Mersakova
authors.affiliation: Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4 A, Martin, 036 01, Slovakia. marcela.nachajova@gmail.com.
authors.forename: Marcela
authors.initials: M
authors.lastname: Nachajova
authors.affiliation: Institute of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Szepe
authors.affiliation: Department of Gynecology and Obstetrics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4 A, Martin, 036 01, Slovakia.
authors.forename: Petra Sumichrastova
authors.initials: PS
authors.lastname: Kasajova
authors.affiliation: BioMed Martin-Division of Molecular Medicine, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
authors.forename: Erika
authors.initials: E
authors.lastname: Halasova
mesh_terms: D002277:Carcinoma / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D011230:Precancerous Conditions / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics / Q000378:metabolism*; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000235:genetics / Q000378:metabolism*
keywords: Cervical cancer; DNA; HPV; Methylation