title: Affinity based and molecularly imprinted cryogels: Applications in biomacromolecule purification.
abstract: The publications in macro-molecularly imprinted polymers have increased drastically in recent years with the development of water-based polymer systems. The macroporous structure of cryogels has allowed the use of these materials within different applications, particularly in affinity purification and molecular imprinting based methods. Due to their high selectivity, specificity, efficient mass transfer and good reproducibility, molecularly imprinted cryogels (MICs) have become attractive for researchers in the separation and purification of proteins. In this review, the recent developments in affinity based cryogels and molecularly imprinted cryogels in protein purification are reviewed comprehensively.
authors.affiliation: Hacettepe University, Department of Environmental Engineering, Ankara, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Andaç
authors.affiliation: DSM Food Specialties B.V., Delft, The Netherlands.
authors.forename: Igor Yu
authors.initials: IY
authors.lastname: Galaev
authors.affiliation: Hacettepe University, Department of Chemistry, Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D059985:Cryogels / Q000737:chemistry*; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D059985:Cryogels
keywords: Affinity purification; Biomacromolecules; Molecularly imprinted cryogels