title: Firpronil induced spermotoxicity is associated with oxidative stress, DNA damage and apoptosis in male rats.
abstract: The present study is the first to investigate and characterize the fipronil (FPN) induced spermotoxicity in male rats. Male rats were orally given FPN (2.5, 5.0 and 10 mg/kg/day) for 4 weeks. Epididymal sperms were collected and remaining testis was processed for histopathological evaluation. FPN treatment significantly reduced sperm density, motility, viability and per cent intact acrosome along with concomitant increase in spermatozoa abnormalities. Exposure of FPN caused excessive ROS generation, lipid peroxidation and alteration in mitochondrial membrane potential leading to apoptosis of spermatozoa in dose dependent manner. Higher FPN doses (5 and 10 mg/kg) markedly reduced the DNA integrity of spermatozoa. These data suggest that FPN causes male reproductive toxicity through oxidative stress induced DNA damage and apoptosis of spermatozoa.
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: Saleem
authors.initials: S
authors.lastname: Khan
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: M H
authors.initials: MH
authors.lastname: Jan
authors.affiliation: Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243122, India.
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar 243122, India. Electronic address: agtelang@rediffmail.com.
authors.forename: A G
authors.initials: AG
authors.lastname: Telang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002843:Chromatin / Q000378:metabolism; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism
chemical_list: D002843:Chromatin; D011720:Pyrazoles; D017382:Reactive Oxygen Species; C082360:fipronil
keywords: DNA damage; Fipronil; Lipid peroxidation; Reactive oxygen species; Spermotoxicity