title: Expression and characterization of a cold-adapted, thermotolerant and denaturant-stable GH5 endoglucanase Celal_2753 that withstands boiling from the psychrophilic bacterium Cellulophaga algicola IC166(T).
abstract: OBJECTIVE
  To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166(T).
  
  
  RESULTS
  Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 °C and pH 6. It showed 33 % of the maximum activity at 10 ºC. Its activity increased to 272-316 % in the presence of 0.25-2 M NaCl and KCl at 40 °C. Celal_2753 was stable in the presence of 10 % (v/v) Tween 20, 10 % (v/v) Triton X-100, 16 mM SDS, 6 M urea or 2 M guanidine hydrochloride. Celal_2753 that had been boiled for 5 min recovered 55 % of its initial activity by incubating at 30 °C for 60 min.
  
  
  CONCLUSIONS
  Because of its cold-adapted, thermotolerant and denaturant-stable properties, endoglucanase Celal_2753 is promising in detergent industry and bioethanol production.
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao, 266003, China.|Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, 5 Yushan Road, Qingdao, 266003, China.|School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao, 266003, China.|Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, 5 Yushan Road, Qingdao, 266003, China.|School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.
authors.forename: Wengong
authors.initials: W
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao, 266003, China. fhan@ouc.edu.cn.|Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, 5 Yushan Road, Qingdao, 266003, China. fhan@ouc.edu.cn.|School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China. fhan@ouc.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D003080:Cold Temperature; D003902:Detergents / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D041965:Flavobacteriaceae / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D011189:Potassium Chloride / Q000378:metabolism; D011489:Protein Denaturation / Q000187:drug effects* / Q000528:radiation effects*; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D003902:Detergents; D020536:Enzyme Activators; D012965:Sodium Chloride; D011189:Potassium Chloride; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium
keywords: Cellulophaga algicola; Cold-adaption; Denaturant-stability; Endoglucanase; Psychrophilic bacterium; Salt-activation; Thermotolerance