title: Enhancing the Quality of Antibodies to HIV-1 Envelope by GagPol-Specific Th Cells.
abstract: The importance of Fc-dependent effector functions of Abs induced by vaccination is increasingly recognized. However, vaccination of mice against HIV envelope (Env) induced a skewed Th cell response leading to Env-specific Abs with reduced effector function. To overcome this bias, GagPol-specific Th cells were harnessed to provide intrastructural help for Env-specific B cells after immunization with virus-like particles containing GagPol and Env. This led to a balanced Env-specific humoral immune response with a more inflammatory Fc glycan profile. The increased quality in the Ab response against Env was confirmed by FcγR activation assays. Because the Env-specific Th cell response was also biased in human vaccinees, intrastructural help is an attractive novel approach to increase the efficacy of prophylactic HIV Env-based vaccines and may also be applicable to other particulate vaccines.
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;
authors.forename: Michael
authors.initials: M
authors.lastname: Storcksdieck genannt Bonsmann
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;
authors.forename: Thomas
authors.identifier: http://orcid.org/0000-0001-6746-8650
authors.initials: T
authors.lastname: Niezold
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany; Universitätsklinikum Erlangen, Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91054, Germany;
authors.forename: Vladimir
authors.identifier: http://orcid.org/0000-0002-8600-252X
authors.initials: V
authors.lastname: Temchura
authors.affiliation: U.S. Military HIV Research Program, Silver Spring, MD 20910;
authors.forename: Franco
authors.initials: F
authors.lastname: Pissani
authors.affiliation: Department of Medical Microbiology and Hygiene, Institute of Virology, University Medical Center Freiburg, Freiburg 79106, Germany;
authors.forename: Katrin
authors.initials: K
authors.lastname: Ehrhardt
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and.
authors.forename: Eric P
authors.initials: EP
authors.lastname: Brown
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and.
authors.forename: Nana Yaw
authors.initials: NY
authors.lastname: Osei-Owusu
authors.affiliation: Ichor Medical Systems, Inc., San Diego, CA 92121.
authors.forename: Drew
authors.initials: D
authors.lastname: Hannaman
authors.affiliation: Department of Medical Microbiology and Hygiene, Institute of Virology, University Medical Center Freiburg, Freiburg 79106, Germany;
authors.forename: Hartmut
authors.initials: H
authors.lastname: Hengel
authors.affiliation: Thayer School of Engineering, Dartmouth College, Hanover, NH 03755; and.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Ackerman
authors.affiliation: U.S. Military HIV Research Program, Silver Spring, MD 20910;
authors.forename: Hendrik
authors.initials: H
authors.lastname: Streeck
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;
authors.forename: Ghulam
authors.initials: G
authors.lastname: Nabi
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany;
authors.forename: Matthias
authors.initials: M
authors.lastname: Tenbusch
authors.affiliation: Department of Molecular and Medical Virology, Ruhr-University Bochum, Bochum 44801, Germany; Universitätsklinikum Erlangen, Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91054, Germany; klaus.ueberla@fau.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Überla
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D057809:HEK293 Cells; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000276:immunology*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus