title: Genomic inbreeding coefficients based on the distribution of the length of runs of homozygosity in a closed line of Iberian pigs.
abstract: BACKGROUND
  The increasing availability of DNA markers provides new metrics of inbreeding based on single nucleotide polymorphisms (SNPs), i.e. molecular inbreeding or the proportion of runs of homozygosity (ROH), as alternatives to traditional pedigree-based inbreeding coefficients. However, none of these metrics incorporate the length of ROH as an indicator of recent inbreeding. Novel inbreeding coefficients that incorporate length of ROH as a random variable with an associated density are investigated.
  
  
  METHODS
  New inbreeding metrics based on the distribution of the length of ROH are proposed: (1) the Kolmolgorov-Smirnov test, (2) a function of the quantiles of the cumulative distribution function of an individual versus the population, and (3) fitting of an exponential distribution to ROH lengths (mean, variance, and the probability of drawing at random a ROH larger than a given threshold). The new inbreeding and pedigree-based metrics were compared using 217 sows of an Iberian line that belong to three groups: C1 (conservation), C2 (conservation derived from C1), and S (selected and derived from C1), with complete pedigrees and genotyped for 35,023 SNPs.
  
  
  RESULTS
  Correlations between pedigree-based and the new genomic inbreeding coefficients ranged from 0.22 to 0.72 but most ranged from 0.60 to 0.70. The correlation between quantile chromosomal inbreeding coefficients (using molecular information of just one chromosome at the time) and chromosomal length was 0.84 (SE = 0.14), supporting the hypothesis that these coefficients incorporate information on ROH length as an indication of recent inbreeding. Kolmogorov-Smirnov and exponential chromosomal inbreeding coefficients were also correlated with chromosomal length (0.57). Chromosome 1 had the largest quantile ROH inbreeding coefficient (largest ROH sizes), whereas chromosome 10 had the lowest (shortest ROH sizes). Selection for lean growth increased ROH-based inbreeding coefficients for group S when compared to unselected groups C1 and C2. At the chromosomal level, this comparison showed that the level of autozygosity and the length of ROH for most of the autosomes increased in the selection line.
  
  
  CONCLUSIONS
  Quantile and exponential probability inbreeding coefficients using ROH length as a random variable provide additional information about recent inbreeding compared to existing inbreeding coefficients such as molecular, pedigree-based or total ROH content inbreeding coefficients.
authors.affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña km 7, 28040, Madrid, Spain. gomez.luis@inia.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Gomez-Raya
authors.affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña km 7, 28040, Madrid, Spain. valdo@inia.es.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rodríguez
authors.affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña km 7, 28040, Madrid, Spain. barragan@inia.es.
authors.forename: Carmen
authors.initials: C
authors.lastname: Barragán
authors.affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra. de La Coruña km 7, 28040, Madrid, Spain. silio@inia.es.
authors.forename: Luis
authors.initials: L
authors.lastname: Silió
mesh_terms: D000465:Algorithms; D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics*; D006720:Homozygote; D007178:Inbreeding; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D013552:Swine / Q000235:genetics*