title: Suppression of Mammary Carcinogenesis Through Early Exposure to Dietary Lipotropes Occurs Primarily In Utero.
abstract: The study determined whether feeding during lactation affects the suppressive effect of maternal dietary lipotropes (i.e., methionine, choline, folate, and vitamin B12) on mammary carcinogenesis. Pregnant Sprague-Dawley rats were randomly allocated to the control diet during pregnancy and lactation (CC), lipotropes-fortified diet during pregnancy (LC), lipotropes-fortified diet during pregnancy plus lactation (LL), or lipotropes-fortified diet during lactation (CL). Randomly selected female offspring from each group were injected intraperitoneally with 50 mg/kg body weight of N-nitroso-N-methylurea at 50 days of age to induce mammary tumors. The LC and LL diets significantly increased tumor latency and survival (P < 0.05). Tumor volumes were significantly suppressed in LC and LL offspring as compared with the CC and CL pups (3759.1 ± 563.0 and 3603.7 ± 526.1 vs. 7465.0 ± 941.1 and 5219.3 ± 759.8 mm(3), respectively; P < 0.05). Both LC and LL lowered tumor multiplicity as compared with CC and CL (P < 0.05). The LC and LL diets repressed transcription of histone deacetylase (HDAC) 1 as well as total HDAC enzyme activity as compared with CC and CL diets (P < 0.05). Data suggest that the tumor suppressive effect of maternal dietary lipotropes is primarily in utero and may be linked to regulation of proteins involved in chromatin remodeling.
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Mabasa
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Kyongshin
authors.initials: K
authors.lastname: Cho
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Choi
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Courtney L
authors.initials: CL
authors.lastname: Crane
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Kwangbog
authors.initials: K
authors.lastname: Cho
authors.affiliation: b Department of Chemistry and Biochemistry , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Raushan K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: a Department of Animal Sciences , North Dakota State University , Fargo , North Dakota , USA.
authors.forename: Chung S
authors.initials: CS
authors.lastname: Park
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002794:Choline / Q000008:administration & dosage; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects*; D004032:Diet; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism; D007774:Lactation; D015674:Mammary Neoplasms, Animal / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D008431:Maternal-Fetal Exchange; D008715:Methionine / Q000008:administration & dosage; D008770:Methylnitrosourea / Q000008:administration & dosage; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014805:Vitamin B 12 / Q000008:administration & dosage
chemical_list: D008770:Methylnitrosourea; D005492:Folic Acid; D008715:Methionine; D006655:Histone Deacetylases; D002794:Choline; D014805:Vitamin B 12