title: A zinc finger protein that regulates oligodendrocyte specification, migration and myelination in zebrafish.
abstract: Precise control of oligodendrocyte migration and development is crucial for myelination of axons in the central nervous system (CNS), but important questions remain unanswered about the mechanisms controlling these processes. In a zebrafish screen for myelination mutants, we identified a mutation in zinc finger protein 16-like (znf16l). znf16l mutant larvae have reduced myelin basic protein (mbp) expression and reduced CNS myelin. Marker, time-lapse and ultrastructural studies indicated that oligodendrocyte specification, migration and myelination are disrupted in znf16l mutants. Transgenic studies indicated that znf16l acts autonomously in oligodendrocytes. Expression of Zfp488 from mouse rescued mbp expression in znf16l mutants, indicating that these homologs have overlapping functions. Our results defined the function of a new zinc finger protein with specific function in oligodendrocyte specification, migration and myelination in the developing CNS.
authors.affiliation: Department of Developmental Biology, Stanford University, CA 94305, USA.
authors.forename: Harwin
authors.initials: H
authors.lastname: Sidik
authors.affiliation: Department of Developmental Biology, Stanford University, CA 94305, USA wtalbot@stanford.edu.
authors.forename: William S
authors.initials: WS
authors.lastname: Talbot
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002352:Carrier Proteins / Q000235:genetics; D019070:Cell Lineage; D002465:Cell Movement; D002490:Central Nervous System / Q000196:embryology; D004268:DNA-Binding Proteins; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D009154:Mutation; D009186:Myelin Sheath / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology*; D012097:Repressor Proteins / Q000235:genetics; D015398:Signal Transduction; D013997:Time Factors; D014157:Transcription Factors; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C000607898:ZNF16l protein, zebrafish; D029961:Zebrafish Proteins; C474810:Zfp24 protein, mouse; C583020:Zfp488 protein, mouse; D049452:Green Fluorescent Proteins
keywords: Myelin; Oligodendrocyte; Zebrafish