title: Dressing with epigallocatechin gallate nanoparticles for wound regeneration.
abstract: Several reagents have been studied to overcome the problems encountered with antiseptic use, such as moderate cutaneous wound cytotoxicity and skin thinning. We successfully prepared a gelatin/chitosan/epigallocatechin gallate nanoparticle incorporated in a poly(γ-glutamic acid)/gelatin hydrogel, which comprised activated carbon fibers with gentamicin, to fabricate a sandwiched dressing to enhance wound regeneration. The inner layer of activated carbon fibers with gentamicin was designed to prevent bacterial infection, and the outer layer of gelatin/chitosan/epigallocatechin gallate nanoparticles incorporated in a poly(γ-glutamic acid)/gelatin hydrogel was designed to prevent inflammation and facilitate reepithelialization. An in vitro study demonstrated that the dressing effectively inhibited target microorganisms, and scanning electron microscope and confocal laser scanning microscope indicated that the nanoparticles were homogeneously dispersed and migrated into the hydrogel. The in vivo study reported that the sandwiched dressing, comprising the poly(γ-glutamic acid)/gelatin hydrogel, was easy to remove from the wound and facilitated wound tissue regeneration and accelerated healing process.
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Yu-Hsin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Bio-Medical Carbon Technology Co., Ltd., Taichung, Taiwan.
authors.forename: Jui-Hsiang
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Plastic Surgery, China Medical University Hospital, Taichung, Taiwan.|School of Medicine, China Medical University, Taichung, Taiwan.|Department of Plastic Surgery, Tainan Municipal An-Nan Hospital, Tainan, Taiwan.
authors.forename: Tzong-Shiun
authors.initials: TS
authors.lastname: Li
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
authors.forename: Shih-Hao
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Lab of Biomaterials, School of Chinese Medicine, China Medical University, Taichung, Taiwan.|Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung, Taiwan.
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
authors.affiliation: Bio-Medical Carbon Technology Co., Ltd., Taichung, Taiwan.
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Chung
authors.affiliation: Department of Materials Science and Engineering, Feng Chia University, Taichung, Taiwan.
authors.forename: Tse-Hao
authors.initials: TH
authors.lastname: Ko
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001458:Bandages; D001672:Biocompatible Materials / Q000494:pharmacology; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D008297:Male; D008422:Materials Testing; D053758:Nanoparticles; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D062326:Re-Epithelialization; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000473:pathology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005839:Gentamicins; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002392:Catechin; D005780:Gelatin; D048271:Chitosan; C045651:epigallocatechin gallate