title: Comparison of Field and Laboratory-Based Tests for Behavioral Response of Aedes aegypti (Diptera: Culicidae) to Repellents.
abstract: The repellent and irritant effects of three essential oils-clove, hairy basil, and sweet basil-were compared using an excito-repellency test system against an insecticide-resistant strain of Aedes aegypti (L.) females from Pu Teuy, Kanchanaburi Province. DEET was used as the comparison standard compound. Tests were conducted under field and controlled laboratory conditions. The most marked repellent effect (spatial noncontact assay) among the three test essential oils was exhibited by sweet basil, Ocimum basilicum L. (53.8% escaped mosquitoes in 30-min exposure period) under laboratory conditions while hairy basil, Ocimum americanum L. and clove, Syzygium aromaticum (L.) Merill et. L.M. Perry from laboratory tests and sweet basil from field tests were the least effective as repellents (0-14%). In contrast, the contact assays measuring combined irritancy (excitation) and repellency effects found the best contact irritant response to hairy basil and DEET in field tests, whereas all others in laboratory and field were relatively ineffective in stimulating mosquitoes to move out the test chambers (0-5.5%). All three essential oils demonstrated significant differences in behavioral responses between field and laboratory conditions, whereas there was no significant difference in contact and noncontact assays for DEET between the two test conditions (P > 0.05).
authors.affiliation: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. faasthc@ku.ac.th.
authors.forename: Sunaiyana
authors.initials: S
authors.lastname: Sathantriphop
authors.affiliation: Department of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kamphaeng Saen Campus, Kasetsart University, Nakhon Pathom 73140, Thailand.
authors.forename: Monthathip
authors.initials: M
authors.lastname: Kongmee
authors.affiliation: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Krajana
authors.initials: K
authors.lastname: Tainchum
authors.affiliation: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
authors.forename: Kornwika
authors.initials: K
authors.lastname: Suwansirisilp
authors.affiliation: Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Patumthani 12110, Thailand.
authors.forename: Unchalee
authors.initials: U
authors.lastname: Sanguanpong
authors.affiliation: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. Public Health & Malaria Control Department, International SOS, Kuala Kencana, Papua 99920, Indonesia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bangs
authors.affiliation: Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand. faasthc@ku.ac.th.
authors.forename: Theeraphap
authors.initials: T
authors.lastname: Chareonviriyaphap
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003671:DEET / Q000494:pharmacology; D005260:Female; D007302:Insect Repellents / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology*
chemical_list: D007302:Insect Repellents; D009822:Oils, Volatile; D003671:DEET
keywords: Aedes aegypti; DEET; essential oil; excito-repellency; response