title: Naringin suppresses the metabolism of A375 cells by inhibiting the phosphorylation of c-Src.
abstract: Elevation of glycolysis, increase in lactic acid production, and enhancement of mitochondrial biogenesis are all the changes of energy metabolism of melanoma cells. Melanoma cells' metabolism and energy production networks play an important role in cancer proliferation, survival, motility, invasiveness, metastasis, and angiogenesis. Since the Warburg theory was put forward in the 1930s, more researchers focus on finding new ways for effectively eliminating cancer cells by targeting their energy metabolism. In this study, we found naringin has the inhibitory effects on the glucose metabolism of A375 cells, a melanoma cell line, in a concentration-dependent manner. We also found that naringin could significantly reduce the phosphorylation of c-Src. In summary, we demonstrated that naringin inhibits the malignant phenotype of A375 cells by suppressing c-Src and its downstream signaling pathway. More importantly, we provide the novel mechanism that, as a natural inhibitor of c-Src, naringin could be an effective candidate for the treatment of melanoma.
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, People's Republic of China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Guo
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hui
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, People's Republic of China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Wang
authors.affiliation: Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, 83#Wenhua Road, Shenhe District, Shenyang, Liaoning, 110016, People's Republic of China. luzongzhengxing@sina.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Tao
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015153:Blotting, Western; D000081247:CSK Tyrosine-Protein Kinase; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects* / Q000235:genetics; D044950:Flavanones / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013963:Thyroid Hormones / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism*; D000097810:Thyroid Hormone-Binding Proteins
chemical_list: D000081247:CSK Tyrosine-Protein Kinase; D002352:Carrier Proteins; D044950:Flavanones; D005947:Glucose; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008565:Membrane Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D013963:Thyroid Hormones; D019061:src-Family Kinases; D000097810:Thyroid Hormone-Binding Proteins; C497442:HIF1A protein, human; C000631346:CSK protein, human; C005274:naringin
keywords: A375 cells; Metabolism; Naringin; Signaling pathway; c-Src