title: Fibronectin-binding protein TDE1579 affects cytotoxicity of Treponema denticola.
abstract: While FbpA, a family of bacterial fibronectin (FN) binding proteins has been studied in several gram-positive bacteria, the gram-negative Treponema denticola, an anaerobic periodontal pathogen, also has an overlooked fbp gene (tde1579). In this research, we confirm that recombinant Fbp protein (rFbp) of T. denticola binds human FN with a Kdapp of 1.5 × 10(-7) M and blocks the binding of T. denticola to FN in a concentration-dependent manner to a level of 42%. The fbp gene was expressed in T. denticola. To reveal the roles of fbp in T. denticola pathogenesis, an fbp isogenic mutant was constructed. The fbp mutant had 51% reduced binding ability to human gingival fibroblasts (hGF). When hGF were challenged with T. denticola, the fbp mutant caused less cell morphology change, had 50% reduced cytotoxicity to hGF, and had less influence on the growth of hGF cells.
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA. Electronic address: xux@uthscsa.edu.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA; Department of Periodontology, Tufts University School of Dental Medicine, Boston, MD, 02111, USA.
authors.forename: Bjorn
authors.initials: B
authors.lastname: Steffensen
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
authors.forename: Trista K
authors.initials: TK
authors.lastname: Robichaud
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
authors.forename: Margarita
authors.initials: M
authors.lastname: Mikhailova
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
authors.forename: Veronica
authors.initials: V
authors.lastname: Lai
authors.affiliation: Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Montgomery
authors.affiliation: Department of Developmental Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.
authors.forename: Lianrui
authors.initials: L
authors.lastname: Chu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D048373:Treponema denticola / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014211:Treponemal Infections / Q000378:metabolism* / Q000382:microbiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D005353:Fibronectins
keywords: Fbp; Fibronectin binding protein; Gene mutant; Periodontal disease; Treponema denticola