title: Melatonin Regulates Oxidative Stress Initiated by Freund's Complete Adjuvant.
abstract: Melatonin is a hormone with strong antioxidant properties. In this experiment, Freund's complete adjuvant was used as a stressogenic substance given to laboratory outbred mice, whereas melatonin was investigated as a protectant against the stressogenic effect. Levels of low molecular weight antioxidants, thiobarbituric acid reactive substances, and tumor necrosis factor α and activity of glutathione reductase were determined in blood from the animals. Surprisingly, melatonin was not involved in direct regulation of antioxidants, thiobarbituric acid reactive substances and tumor necrosis factor α. On the other hand, melatonin regulated glutathione reductase activity. We can conclude on regulation of metabolism caused by melatonin in the model. The effect was more important than the expected regulation of immunity and basal oxidative homeostasis.
authors.affiliation: Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic. miroslav.pohanka@gmail.com.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pohanka
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.|Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
authors.forename: Branislav
authors.initials: B
authors.lastname: Ruttkay-Nedecký
authors.affiliation: Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Fusek
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.|Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Adam
authors.affiliation: Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.|Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
authors.forename: René
authors.initials: R
authors.lastname: Kizek
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D008550:Melatonin / Q000494:pharmacology* / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000276:Adjuvants, Immunologic; D000975:Antioxidants; D005620:Freund's Adjuvant; D008550:Melatonin
keywords: Antioxidant; Epiphysis; Hormone; Melatonin; Oxidative stress; Reactive oxygen species