title: Leukemia Inhibitory Factor (LIF) Inhibition during Mid-Gestation Impairs Trophoblast Invasion and Spiral Artery Remodelling during Pregnancy in Mice.
abstract: The placenta forms the interface between the maternal and fetal circulation and is critical for the establishment of a healthy pregnancy. Trophoblast cell proliferation, migration and invasion into the endometrium are fundamental events in the initiation of placentation. Leukemia inhibitory factor (LIF) has been shown to promote trophoblast invasion in vitro, however its precise role in trophoblast invasion in vivo is unknown. We hypothesized that LIF would be required for normal trophoblast invasion and spiral artery remodeling in mice. Both LIF and its receptor (LIFRα) co-localized with cytokeratin-positive invasive endovascular extravillous trophoblasts (EVT) in mouse implantation sites during mid-gestation. Temporally blocking LIF action during specific periods of placental development via administration of our unique LIFRα antagonist, PEGLA, resulted in abnormal trophoblast invasion and impaired spiral artery remodeling compared to PEG control. PEGLA-treated mouse decidual vessels were characterized by retention of α-smooth muscle actin (αSMA)-positive vascular smooth muscle cells (VSMCs), while PEG control decidual vessels were remodelled by cytokeratin-positive trophoblasts. LIF blockade did not alter F4/80-positive decidual macrophage numbers between treatment groups, but resulted in down-regulation of decidual transcript levels of monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10), which are important immune cell activation factors that promote spiral artery remodeling during pregnancy. Our data suggest that LIF plays an important role in trophoblast invasion in vivo and may facilitate trophoblast-decidual-immune cell cross talk to enable adequate spiral artery remodeling.
authors.affiliation: The Hudson Institute, Melbourne, Australia; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Australia.
authors.forename: Amy
authors.initials: A
authors.lastname: Winship
authors.affiliation: The Hudson Institute, Melbourne, Australia.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Correia
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, The University of Melbourne, Melbourne, Australia.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhang
authors.affiliation: The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia; Department of Medical Biology, The University of Melbourne, Melbourne, Australia.
authors.forename: Nicos A
authors.initials: NA
authors.lastname: Nicola
authors.affiliation: The Hudson Institute, Melbourne, Australia; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Australia.
authors.forename: Evdokia
authors.initials: E
authors.lastname: Dimitriadis
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects* / Q000502:physiology*; D018932:Chemokine CCL2 / Q000235:genetics; D050822:Cytokine Receptor gp130 / Q000235:genetics; D003656:Decidua / Q000098:blood supply / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D016753:Interleukin-10 / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010929:Placentation / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011247:Pregnancy; D021381:Protein Transport / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053606:Receptors, OSM-LIF / Q000235:genetics / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology* / Q000187:drug effects*; D066253:Vascular Remodeling / Q000187:drug effects*
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; C494200:Il6st protein, mouse; D053605:Leukemia Inhibitory Factor; D012333:RNA, Messenger; D053606:Receptors, OSM-LIF; D016753:Interleukin-10; D050822:Cytokine Receptor gp130; D011092:Polyethylene Glycols