title: The role of host microfilaments and microtubules during opsonin-independent interactions of Cryptococcus neoformans with mammalian lung cells.
abstract: The purpose of this investigation was to characterise the interactions of Cryptococcus neoformans with mammalian host alveolar epithelial cells and alveolar macrophages, with emphasis on the roles of the cryptococcal capsule and the host cell cytoskeletons. The adherence and internalisation of C. neoformans into mammalian lung cells and the roles of host cell cytoskeletons in host-pathogen interactions were studied using in vitro models coupled with a differential fluorescence assay, fluorescence staining, immunofluorescence and drug inhibition of actin and microtubule polymerisation. Under conditions devoid of opsonin and macrophage activation, C. neoformans has a high affinity towards MH-S alveolar macrophages, yet associated poorly to A549 alveolar epithelial cells. Acapsular C. neoformans adhered to and internalised into the mammalian cells more effectively compared to encapsulated cryptococci. Acapsular C. neoformans induced prominent actin reorganisation at the host-pathogen interface in MH-S alveolar macrophages, but minimally affected actin reorganisation in A549 alveolar epithelial cells. Acapsular C. neoformans also induced localisation of microtubules to internalised cryptococci in MH-S cells. Drug inhibition of actin and microtubule polymerisation both reduced the association of acapsular C. neoformans to alveolar macrophages. The current study visualises and confirms the interactions of C. neoformans with mammalian alveolar cells during the establishment of infection in the lungs. The acapsular form of C. neoformans effectively adhered to and internalised into alveolar macrophages by inducing localised actin reorganisation, relying on the host's actin and microtubule activities.
authors.affiliation: School of Biosciences, Taylor's University, Subang, Selangor, Malaysia.
authors.forename: K K
authors.initials: KK
authors.lastname: Choo
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: P P
authors.initials: PP
authors.lastname: Chong
authors.affiliation: School of Biosciences, Taylor's University, Subang, Selangor, Malaysia.
authors.forename: A S H
authors.initials: AS
authors.lastname: Ho
authors.affiliation: School of Biosciences, Taylor's University, Subang, Selangor, Malaysia. PhelimVoonChen.Yong@taylors.edu.my.
authors.forename: P V C
authors.initials: PV
authors.lastname: Yong
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D003455:Cryptococcus neoformans / Q000502:physiology*; D004705:Endocytosis; D004847:Epithelial Cells / Q000382:microbiology / Q000502:physiology*; D062607:Fungal Capsules / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D008264:Macrophages / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*