title: Aquimarina hainanensis sp. nov., isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae.
abstract: One novel Gram-stain-negative, long rod-shaped, non-spore-forming, non-motile, non-flagellated and strictly aerobic strain, designated M124T, was isolated from diseased Pacific white shrimp Litopenaeus vannamei larvae. Growth occurred at 16-37 °C (optimum 28 °C), in the presence of 2-5 % (w/v) NaCl (optimum 3 %) and at pH 7-8 (optimum pH 7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M124T belonged to the genus Aquimarina and showed highest sequence similarity to Aquimarina penaei P3-1T (96.4 %). The dominant fatty acids of the isolate were iso-C15 : 0 and iso-C17 : 0 3-OH. The major polar lipids comprised phosphatidylethanolamine, one unknown aminolipid, three unknown phospholipids, two unknown glycolipids and one unknown polar lipid. The major respiratory quinone was menaquinone 6 (MK-6). The DNA G+C content of strain M124T was 33.7 mol%. Based on the polyphasic analyses in this study, strain M124T is considered to represent a novel species of the genus Aquimarina, for which the name Aquimarina hainanensis sp. nov. is proposed. The type strain is M124T ( = KCTC 42423T = MCCC 1K00498T).
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Tongwei Co., Ltd, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: Tongwei Co., Ltd, PR China.
authors.forename: Mingchao
authors.initials: M
authors.lastname: Yu
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D007814:Larva; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6