title: Sebocytes differentially express and secrete adipokines.
abstract: In addition to producing sebum, sebocytes link lipid metabolism with inflammation at a cellular level and hence, greatly resemble adipocytes. However, so far no analysis was performed to identify and characterize the adipocyte-associated inflammatory proteins, the members of the adipokine family in sebocytes. Therefore, we determined the expression profile of adipokines [adiponectin, interleukin (IL) 6, resistin, leptin, serpin E1, visfatin, apelin, chemerin, retinol-binding protein 4 (RBP4) and monocyte chemoattractant protein 1 (MCP1)] in sebaceous glands of healthy and various disease-affected (acne, rosacea, melanoma and psoriasis) skin samples. Sebaceous glands in all examined samples expressed adiponectin, IL6, resistin, leptin, serpin E1 and visfatin, but not apelin, chemerin, RBP4 and MCP1. Confirming the presence of the detected adipokines in the human SZ95 sebaceous gland cell line we further characterized their expression and secretion patterns under different stimuli mimicking bacterial invasion [by using Toll-like receptor (TLR)2 and 4 activators], or by 13-cis retinoic acid (13CRA; also known as isotretinoin), a key anti-acne agent. With the exception of resistin, the expression of all of the detected adipokines (adiponectin, IL6, leptin, serpin E1 and visfatin) could be further regulated at the level of gene expression, showing a close correlation with the secreted protein levels. Besides providing further evidence on similarities between adipocytes and sebocytes, our results strongly suggest that sebocytes are not simply targets of inflammation but may exhibit initiatory and modulatory roles in the inflammatory processes of the skin through the expression and secretion of adipokines.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.|MTA-DE, Public Health Research group of the Hungarian Academy of Sciences, Faculty of Public Health, University of Debrecen, Debrecen, Hungary.
authors.forename: Dóra
authors.initials: D
authors.lastname: Kovács
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Marianna
authors.initials: M
authors.lastname: Lovászi
authors.affiliation: Department of Biochemistry and Molecular Biology, Genomic Medicine and Bioinformatics Core Facility, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Szilárd
authors.initials: S
authors.lastname: Póliska
authors.affiliation: DE-MTA "Lendület" Cellular Physiology Research Group, Department of Physiology, University of Debrecen, Debrecen, Hungary.
authors.forename: Attila
authors.identifier: http://orcid.org/0000-0003-4122-5639
authors.initials: A
authors.lastname: Oláh
authors.affiliation: DE-MTA "Lendület" Cellular Physiology Research Group, Department of Physiology, University of Debrecen, Debrecen, Hungary.|Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Bíró
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Veres
authors.affiliation: Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany.
authors.forename: Christos C
authors.initials: CC
authors.lastname: Zouboulis
authors.affiliation: Unit of Dermatology and Venereology, Department of Medicine, Karolinska lnstitutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Mona
authors.initials: M
authors.lastname: Ståhle
authors.affiliation: MTA-DE, Public Health Research group of the Hungarian Academy of Sciences, Faculty of Public Health, University of Debrecen, Debrecen, Hungary.
authors.forename: Ralph
authors.initials: R
authors.lastname: Rühl
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Remenyik
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.|Unit of Dermatology and Venereology, Department of Medicine, Karolinska lnstitutet, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Dániel
authors.initials: D
authors.lastname: Törőcsik
mesh_terms: D017667:Adipocytes / Q000166:cytology; D054392:Adipokines / Q000378:metabolism*; D052242:Adiponectin / Q000378:metabolism; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000378:metabolism; D020738:Leptin / Q000378:metabolism; D050356:Lipid Metabolism; D052243:Resistin / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology*; D012629:Sebum / Q000378:metabolism; D012867:Skin / Q000378:metabolism
chemical_list: C499918:ADIPOQ protein, human; D054392:Adipokines; D052242:Adiponectin; C508600:IL6 protein, human; D015850:Interleukin-6; D020738:Leptin; C499624:RETN protein, human; D052243:Resistin
keywords: adipokine; inflammation; sebocyte