title: Characterization of microfouling and corrosive bacterial community of a firewater distribution system.
abstract: This investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. Conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. The isolates were characterized by 16S rRNA gene sequencing and the biofilm as well as the corrosion characteristics of the isolates were evaluated. Pseudomonas aeruginosa and Bacillus cereus were predominantly observed in all the samples analysed. Denaturing gradient gel electrophoresis (DGGE) was carried out for the various samples of firewater system (FWS) and the high intensity bands were sequenced to identify the predominant bacteria. Bacterial groups such as Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes were identified. Biofilm thickness was recorded using confocal scanning laser microscopy (CSLM). This was the first study to report Lysinibacillus fusiformis in a firewater system and its role in iron corrosion. Sulphidogenic bacteria Tissierella sp. and Clostridium bifermentans generated sulphides in the range of 400-900 ppm. Significant corrosion rates of carbon steel (CS) coupons were observed up to 4.3 mpy. C. bifermentans induced more localized corrosion in CS with a pit diameter of 50 μm. Overall, the data on the characterization of the fouling bacteria, their biofilm forming potential and subsequent metal deterioration studies supported in designing an effective water treatment program.
authors.affiliation: Department of Biotechnology, Pondicherry University, Puducherry 605 014, India. Electronic address: balaicon@gmail.com.
authors.forename: Balamurugan
authors.initials: B
authors.lastname: Palaniappan
authors.affiliation: Water & Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam 603 102, India. Electronic address: tsrmicro@gmail.com.
authors.forename: Subba Rao
authors.initials: SR
authors.lastname: Toleti
mesh_terms: D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D018441:Biofilms; D057219:Biofouling; D003343:Corrosion; D058645:Denaturing Gradient Gel Electrophoresis; D005390:Fires; D005618:Fresh Water / Q000382:microbiology*; D018613:Microscopy, Confocal; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013232:Steel / Q000737:chemistry; D018508:Water Purification / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S; D013232:Steel
keywords: Bacteria; Confocal scanning laser microscopy; Corrosion; Denaturing gradient gel electrophoresis; Fouling community; Freshwater