title: Comparative study between biopsy and brushing sampling methods for detection of human papillomavirus in oral and oropharyngeal cavity lesions.
abstract: BACKGROUND
  Many epidemiological studies have suggested that human papillomavirus (HPV), especially type 16, is involved in the genesis of squamous cell carcinoma of the oral cavity and oropharynx, especially in young, non-smoking patients; thus, its detection in lesions in this region is important.
  
  
  OBJECTIVE
  To clarify the capacity of the brushing sampling method to detect the presence of HPV in oral or oropharyngeal lesions through polymerase chain reaction (PCR) testing, and to compare the results with those obtained by biopsy.
  
  
  METHODS
  Prospective study of adult patients with oral or oropharyngeal lesions assessed by PCR, comparing biopsy specimens with samples obtained by the brushing method. The study was approved by the Research Ethics Committee of the institution.
  
  
  RESULTS
  A total of 35 sample pairs were analyzed, but 45.7% of the brushing samples were inadequate (16/35) and, thus, only 19 pairs could be compared. There was agreement of results in 94.7% (18/19) of the pairs, with HPV identified in 16 of them. HPV DNA was detected in 8.6% (3/35) of biopsy and 5.7% (2/35) of brushing samples.
  
  
  CONCLUSIONS
  There was no statistically significant difference between the two methods, but the brushing sampling method showed a higher number of inadequate samples, suggesting that it is an unreliable method for surveillance.
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil; Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Electronic address: mariseorl@globo.com.
authors.forename: Marise da Penha Costa
authors.initials: Mda P
authors.lastname: Marques
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil; Department of Otorhinolaryngology, Irmandade da Santa Casa de Misericórdia de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ivo
authors.initials: I
authors.lastname: Bussoloti Filho
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil; Faculdade de Medicina de Jundiaí, Jundiaí, SP, Brazil.
authors.forename: Lia Mara
authors.initials: LM
authors.lastname: Rossi
authors.affiliation: Instituto Nacional de Ciência e Tecnologia das Doenças Associadas ao Papilomavírus, São Paulo, SP, Brazil.
authors.forename: Maria Antonieta
authors.initials: MA
authors.lastname: Andreoli
authors.affiliation: Faculdade de Ciências Médicas, Santa Casa de São Paulo (FCMSCSP), São Paulo, SP, Brazil.
authors.forename: Natália Oliveira
authors.initials: NO
authors.lastname: Cruz
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy / Q000379:methods; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D061809:Human Papillomavirus DNA Tests; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000821:virology*; D009959:Oropharyngeal Neoplasms / Q000175:diagnosis / Q000821:virology*; D009960:Oropharynx / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral
keywords: Boca; Human papillomavirus DNA tests; Mouth; Orofaringe; Oropharynx; Polymerase chain reaction; Reação de polimerase em cadeia; Testes de DNA para papilomavírus humano