title: Parallel microscope-based fluorescence, absorbance and time-of-flight mass spectrometry detection for high performance liquid chromatography and determination of glucosamine in urine.
abstract: A parallel microscope-based laser-induced fluorescence (LIF), ultraviolet-visible absorbance (UV) and time-of-flight mass spectrometry (TOF-MS) detection for high performance liquid chromatography (HPLC) was achieved and used to determine glucosamine in urines. First, a reliable and convenient LIF detection was developed based on an inverted microscope and corresponding modulations. Parallel HPLC-LIF/UV/TOF-MS detection was developed by the combination of preceding Microscope-based LIF detection and HPLC coupled with UV and TOF-MS. The proposed setup, due to its parallel scheme, was free of the influence from photo bleaching in LIF detection. Rhodamine B, glutamic acid and glucosamine have been determined to evaluate its performance. Moreover, the proposed strategy was used to determine the glucosamine in urines, and subsequent results suggested that glucosamine, which was widely used in the prevention of the bone arthritis, was metabolized to urines within 4h. Furthermore, its concentration in urines decreased to 5.4mM at 12h. Efficient glucosamine detection was achieved based on a sensitive quantification (LIF), a universal detection (UV) and structural characterizations (TOF-MS). This application indicated that the proposed strategy was sensitive, universal and versatile, and it was capable of improved analysis, especially for analytes with low concentrations in complex samples, compared with conventional HPLC-UV/TOF-MS.
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China. Electronic address: bx@mail.ccnu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Nie
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Ren-Qiang
authors.initials: RQ
authors.lastname: Sun
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Yu-Jiao
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D005944:Glucosamine / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods
chemical_list: D005944:Glucosamine
keywords: Glucosamine; High performance liquid chromatography; Microscope-based fluorescence; Time-of-flight mass spectrometry; Ultraviolet–visible absorbance