title: Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry.
abstract: Labelling strategy plays an important role in mass spectrometry (MS) based glycan analysis due to the high hydrophilicity and low ionization efficiency of glycans. Ten hydrazino-s-triazine based labelling reagents were synthesized under facile and controllable conditions for highly sensitive liquid chromatography-electrospray mass spectrometry glycan analysis in this work. Attached to N-glycans through non-reductive reactions, these new labelling reagents were evaluated in aspect of the differently enhanced glycan response to mass spectrometry. Three of the ten labelling reagents demonstrated to be reliable and remarkable for glycan analysis with satisfactory linearity and lowered limits of detection using maltoheptaose (DP7) as model. Furthermore, the most optimal labelling reagent was taken as an example for highly sensitive profiling of N-linked glycans both cleaved from chicken avidin and glycoproteins in human serum, indicating prospective availability for these labelling reagents in frontier of glycomics researches.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Zhao
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Petrochemical Research Institute, Petrochina Company Limited, Beiwuchun Road, No. 25, Haidian District, Beijing 100083, China.
authors.forename: Jing-Xin
authors.initials: JX
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. Electronic address: zhouyl@pku.edu.cn.
authors.forename: Ying-Lin
authors.initials: YL
authors.lastname: Zhou
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, China. Electronic address: zxx@pku.edu.cn.
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D001360:Avidin / Q000737:chemistry; D002853:Chromatography, Liquid; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014227:Triazines / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D007202:Indicators and Reagents; D011134:Polysaccharides; D014227:Triazines; D001360:Avidin
keywords: Controllable synthesis; Glycan; Glycomics profiling; Labelling reagent; Liquid chromatography–electrospray mass spectrometry (LC–ESI-MS)