title: Rootstock-scion interaction affecting citrus response to CTV infection: a proteomic view.
abstract: Citrus tristeza virus (CTV) is the causal agent of various diseases with dramatic effects on citrus crops worldwide. Most Citrus species, grown on their own roots, are symptomless hosts for many CTV isolates. However, depending on different scion-rootstock combination, CTV infection should result in distinct syndromes, being 'tristeza' the more severe one, leading to a complete decline of the susceptible plants in a few weeks. Transcriptomic analyses revealed several genes involved either in defense response, or systemic acquired resistance, as well as transcription factors and components of the phosphorylation cascades, to be differentially regulated during CTV infection in Citrus aurantifolia species. To date little is known about the molecular mechanism of this host-pathogen interaction, and about the rootstock effect on citrus response to CTV infection. In this work, the response to CTV infection has been investigated in tolerant and susceptible scion-rootstock combinations by two-dimensional gel electrophoresis (2DE). A total of 125 protein spots have been found to be differently accumulated and/or phosphorylated between the two rootstock combinations. Downregulation in tolerant plants upon CTV infection was detected for proteins involved in reactive oxygen species (ROS) scavenging and defense response, suggesting a probable acclimation response able to minimize the systemic effects of virus infection. Some of these proteins resulted to be modulated also in absence of virus infection, revealing a rootstock effect on scion proteome modulation. Moreover, the phospho-modulation of proteins involved in ROS scavenging and defense response, further supports their involvement either in scion-rootstock crosstalk or in the establishment of tolerance/susceptibility to CTV infection.
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Laino
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per l'Agrumicoltura e le Colture Mediterranee, Acireale (CT), Italy.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Russo
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per l'Agrumicoltura e le Colture Mediterranee, Acireale (CT), Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Guardo
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per l'Agrumicoltura e le Colture Mediterranee, Acireale (CT), Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Reforgiato-Recupero
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Rice Research Unit, Vercelli, Italy.
authors.forename: Giampiero
authors.initials: G
authors.lastname: Valè
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Cattivelli
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Genomics Research Centre, Fiorenzuola d'Arda (PC), Italy.
authors.forename: Vita M C
authors.initials: VM
authors.lastname: Moliterni
mesh_terms: D002957:Citrus / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D017790:Closterovirus / Q000502:physiology*; D018556:Crops, Agricultural; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010750:Phosphoproteins / Q000378:metabolism; D024301:Plant Bark / Q000276:immunology / Q000378:metabolism / Q000821:virology; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D057865:Plant Immunity; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism; D018517:Plant Roots / Q000276:immunology / Q000378:metabolism / Q000821:virology; D017510:Protein Folding; D020543:Proteome; D040901:Proteomics; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome