title: Excretory/secretory proteome of 14-day schistosomula, Schistosoma japonicum.
abstract: Schistosomiasis remains a serious public health problem, with 200 million people infected and 779 million people at risk worldwide. The schistosomulum is the early stage of the complex lifecycle of Schistosoma japonicum in their vertebrate hosts, and is the main target of vaccine-induced protective immunity. Excretory/secretory (ES) proteins play a major role in host-parasite interactions and ES protein compositions of schistosomula of S. japonicum have not been characterized to date. In the present study, the proteome of ES proteins from 14 day schistosomula of S. japonicum was analyzed by liquid chromatography/tandem mass spectrometry and 713 unique proteins were finally identified. Gene ontology and pathway analysis revealed that identified proteins were mainly involved in carbohydrate metabolism, degradation, response to stimulus, oxidation-reduction, biological regulation and binding. Flow cytometry analysis demonstrated that thioredoxin peroxidase identified in this study had the effect on inhibiting MHCII and CD86 expression on LPS-activated macrophages. The present study provides insight into the growth and development of the schistosome in the final host and valuable information for screening vaccine candidates for schistosomiasis.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Fu
authors.affiliation: College of Animal Science and Technology, Henran University of Science and Technology, Luoyang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Henan Institute of Science and Technology, Xinxiang, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Han
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Han
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China.
authors.forename: Chuangang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China. Electronic address: hongyang_7@126.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. Electronic address: jjlin@shvri.ac.cn.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid; D019295:Computational Biology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D008070:Lipopolysaccharides / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism; D008297:Male; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D012549:Schistosoma japonicum / Q000378:metabolism*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000469:parasitology; D053719:Tandem Mass Spectrometry
chemical_list: D002241:Carbohydrates; D015801:Helminth Proteins; D008070:Lipopolysaccharides; D020543:Proteome; D011994:Recombinant Proteins
keywords: Excretory/secretory proteins; Macrophages; Proteome; Schistosoma japonicum; Schistosomula; Thioredoxin peroxidase