title: Analysis of Macrophage-Induced Salmonella Persisters.
abstract: A small subpopulation of non-replicating, multidrug-tolerant bacteria is present within clonal populations of many bacterial species. Known as persisters, these bacteria are probably the cause of relapsing infections such as typhoid fever. Formation of non-growing Salmonella persisters is stimulated by macrophage phagocytosis. This chapter outlines methods to identify and study persisters resulting from interactions between bacterial pathogens and their hosts. We use their antibiotic tolerance for isolation and enumeration and developed a method to study the heterogeneity of growth within clonal populations through single-cell analysis.
authors.affiliation: Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Armstrong Road, London, SW7 2AZ, UK.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Fisher
authors.affiliation: Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Armstrong Road, London, SW7 2AZ, UK.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Cheverton
authors.affiliation: Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, South Kensington Campus, Armstrong Road, London, SW7 2AZ, UK. s.helaine@imperial.ac.uk.
authors.forename: Sophie
authors.initials: S
authors.lastname: Helaine
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D018432:Drug Resistance, Multiple / Q000187:drug effects / Q000235:genetics*; D005453:Fluorescence; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D012475:Salmonella / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D059010:Single-Cell Analysis / Q000379:methods*; D013234:Stem Cells / Q000187:drug effects / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Bacterial pathogen; Fluorescence dilution; Macrophages; Persisters; Salmonella; Single-cell analysis