title: Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases.
abstract: A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic.
authors.forename: Agata
authors.initials: A
authors.lastname: Olszewska
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic.
authors.forename: Jitka
authors.initials: J
authors.lastname: Dadová
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Mačková
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, CZ-16610 Prague 6, Czech Republic; Department of Organic and Nuclear Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic. Electronic address: hocek@uochb.cas.cz.
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
mesh_terms: D004247:DNA / Q000032:analysis / Q000378:metabolism*; D053837:DNA Cleavage; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005437:Flucytosine / Q000737:chemistry; D005472:Fluorouracil / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004247:DNA; D005437:Flucytosine; D015252:Deoxyribonucleases, Type II Site-Specific; D005472:Fluorouracil
keywords: DNA; DNA polymerase; Modified nucleotides; Pyrimidine nucleosides; Restriction endonucleases