title: Photo-Activatable Substrates for Site-Specific Differentiation of Stem Cells.
abstract: In this report, a UV sensitive, PEGylated PFSSTKTC (Pro-Phe-Ser-Ser-Thr-Lys-Thr-Cys) peptide was modified on quartz substrate to investigate the spatial controlled differentiation of stem cells. This substrate could restrict the cell adhesion due to the steric hindrance of PEG shell. With UV irradiation, PFSSTKTC became exposed owing to the breakage of o-nitrobenzyl group with the detachment of PEG shell. The irradiation boundary on substrate was stable in the long term. The in vitro osteogenic differentiation results revealed that under the site-specific irradiation, the mesenchymal stem cells (MSCs) could specifically differentiate into osteoblast under the induction of PFSSTKTC peptide. This photoactivatable biomaterial shows great potential for region controllable and precise MSCs differentiation.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.|College of Science, Huazhong Agricultural University , Wuhan 430070, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Han
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Yin
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Jin-Xuan
authors.initials: JX
authors.lastname: Fan
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Feng-Yi
authors.initials: FY
authors.lastname: Cao
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University , Wuhan 430072, China.
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D009579:Nitrobenzoates / Q000737:chemistry / Q000494:pharmacology; D010006:Osteoblasts / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects* / Q000528:radiation effects; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: C518437:2-nitrobenzoate; D001672:Biocompatible Materials; D009579:Nitrobenzoates; D010455:Peptides
keywords: cell adhesion; osteogenic differentiation; photoactivatable; site-specific; stem cells