title: Ultrastructural and cytochemical characterization of T1 and T2 secretory bodies from the tegument of Echinostoma paraensei.
abstract: Trematodes are lined by a syncytial layer that is named the tegument and contains small mitochondria and two different kinds of secretory inclusions. The structure and size of these bodies differs among genera and species. In a previous study, we observed many secretory bodies in the tegument of Echinostoma paraensei and named these bodies the T1 and T2 secretory bodies. No previous studies analyzed the secretory bodies of trematodes from the genus Echinostoma. Thus, the aim of this work was to use electron microscopy and cytochemistry to characterize these secretory bodies and to provide a detailed ultrastructural and morphological picture of these bodies, which are found in the tegument of E. paraensei. After ultrastructural cytochemistry analysis, we showed that both the T1 and T2 secretory bodies of E. paraensei were formed by glycoconjugates. 3D reconstruction confirmed the ovoid form of T1 secretory bodies and the biconcave and thin form of T2 secretory bodies.
authors.affiliation: Pólo Barreto, Universidade Federal do Rio de Janeiro, Campus Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, Rio de Janeiro CEP: 27910-970, Brazil; Laboratório de Biologia de Helmintos Otto Wucherer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil. Electronic address: peraltagoncalves@gmail.com.
authors.forename: Júlia P
authors.initials: JP
authors.lastname: Gonçalves
authors.affiliation: Pólo Barreto, Universidade Federal do Rio de Janeiro, Campus Macaé, Avenida São José do Barreto, 764, São José do Barreto, Macaé, Rio de Janeiro CEP: 27910-970, Brazil.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Oliveira-Menezes
authors.affiliation: Laboratório de Biologia e Parasitologia de Mamíferos Silvestres e Reservatórios, Instituto Oswaldo Cruz, Av. Brasil 4365, 20010-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Maldonado Junior
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Instituto Nacional de Biologia Estrutural e Bioimagem-INBEB, and Centro Nacional de Biologia Estrutural e Bioimagem-CENABIO, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Técia M U
authors.initials: TM
authors.lastname: Carvalho
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Instituto Nacional de Biologia Estrutural e Bioimagem-INBEB, and Centro Nacional de Biologia Estrutural e Bioimagem-CENABIO, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, 21949-900 Rio de Janeiro, RJ, Brazil.
authors.forename: Wanderley
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000825:Animal Structures / Q000166:cytology / Q000648:ultrastructure; D000818:Animals; D004449:Echinostoma / Q000166:cytology* / Q000648:ultrastructure*; D006651:Histocytochemistry; D008854:Microscopy, Electron; D022142:Secretory Vesicles / Q000648:ultrastructure*
keywords: 3D modelling; Cytochemical; Echinostoma paraensei; Secretory bodies; Transmission electron microscopy; Ultrastructure