title: High prevalence of HPV59 in cytologically abnormal cervical samples.
abstract: BACKGROUND
  Cervical cancer is the second most common form of cancer among women. There are over 100 different types of human papillomavirus (HPV), 40 of which are frequently detected in anogenital mucosa. HPV is the primary etiological agent of cervical cancer and is present predominantly in cervical cancers. Thirteen commonly recognized high-risk genotypes have oncogenic potential. The most common high-risk HPV (hrHPV) genotypes in cervical cancer are HPV16 and HPV18, which have the greatest malignancies. The objective of this study was to determine the distribution of hrHPV types in patient samples received at the Mount Sinai Medical Center for routine cytology and HPV testing. In addition, the study compared the sensitivity of the Hybrid Capture 2 (HC2) and Roche Linear Array HPV Genotyping assays.
  
  
  METHODS
  Cytology specimens with abnormal diagnoses were tested for HPV by the Roche Linear Array HPV Genotyping and Digene Hybrid Capture 2 molecular systems.
  
  
  RESULTS
  The Roche Linear Array HPV Genotyping Assay was more sensitive than HC2. Additionally, specimens exhibited higher rates of HPV16, HPV51, and HPV59 infections than HPV16 and HPV18 infections.
  
  
  CONCLUSIONS
  The results demonstrate that geographical distribution of HPV genotypes may play an important role in clinical management of HPV infection, particularly when treating cervical dysplasia and recommending HPV vaccination.
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA. Electronic address: fei.ye@mssm.edu.
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Chan
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Josephine
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Shiquang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Mount Sinai School of Medicine, New York, NY 10029, USA.
authors.forename: Rhoda
authors.initials: R
authors.lastname: Sperling
authors.affiliation: Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA. Electronic address: david.zhang@mssm.edu.
authors.forename: David Y
authors.initials: DY
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000821:virology*
keywords: Cytological classifications; HPV genotype prevalence; HPV genotyping; Roche linear array