title: Bioinspired genotype-phenotype linkages: mimicking cellular compartmentalization for the engineering of functional proteins.
abstract: The idea of compartmentalization of genotype and phenotype in cells is key for enabling Darwinian evolution. This contribution describes bioinspired systems that use in vitro compartments-water-in-oil droplets and gel-shell beads-for the directed evolution of functional proteins. Technologies based on these principles promise to provide easier access to protein-based therapeutics, reagents for processes involving enzyme catalysis, parts for synthetic biology and materials with biological components.
authors.affiliation: Department of Biochemistry , University of Cambridge , 80 Tennis Court Road, Cambridge CB2 1GA , UK.
authors.forename: Liisa D
authors.initials: LD
authors.lastname: van Vliet
authors.affiliation: Department of Biochemistry , University of Cambridge , 80 Tennis Court Road, Cambridge CB2 1GA , UK.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Colin
authors.affiliation: Department of Biochemistry , University of Cambridge , 80 Tennis Court Road, Cambridge CB2 1GA , UK.
authors.forename: Florian
authors.initials: F
authors.lastname: Hollfelder
keywords: antibodies; bioinspiration; cell compartmentalization; emulsion droplets; microfluidics; protein engineering; synthetic biology