title: Integrated miRNA profiling and bioinformatics analyses reveal potential causative miRNAs in gastric adenocarcinoma.
abstract: Gastric cancer (GC) is one of the leading causes of cancer-related deaths throughout China and worldwide. The discovery of microRNAs (miRNAs) has provided a new opportunity for developing diagnostic biomarkers and effective therapeutic targets in GC. By performing microarray analyses of benign and malignant gastric epithelial cell lines (HFE145, NCI-N87, MKN28, RF1, KATO III and RF48), 16 significantly dysregulated miRNAs were found. 11 of these were validated by real-time qRT-PCR. Based on miRWalk online database scans, 703 potential mRNA targets of the 16 miRNAs were identified. Bioinformatic analyses suggested that these dysregulated miRNAs and their predicted targets were principally involved in tumor pathogenesis, MAPK signaling, and apoptosis. Finally, miRNA-gene network analyses identified miRNA-125b as a crucial miRNA in GC development. Taken together, these results develop a comprehensive expression and functional profile of differentially expressed miRNAs related to gastric oncogenesis. This profile may serve as a potential tool for biomarker and therapeutic target identification in GC patients.
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Shenzhen Key Laboratory of Translational Medicine of Tumor, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Department of Pathology, Wuhan University School of Basic Medical Sciences, Hubei, People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Shenzhen Key Laboratory of Micromolecule Innovatal Drugs, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Shenzhen Key Laboratory of Translational Medicine of Tumor, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Medicine/GI Division, Johns Hopkins University and Sidney Kimmel Cancer Center, Baltimore, MD, USA.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: School of Pharmacy, China Medical University, Shenyang, Liaoning, People's Republic of China.
authors.forename: Yudan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Xianling
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Mengting
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Zhenfu
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Shenzhen Key Laboratory of Translational Medicine of Tumor, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Center for High Performance Computing, Shenzhen Institutes of Advanced Technology, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Pathology, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.|Shenzhen Key Laboratory of Translational Medicine of Tumor, The Shenzhen University School of Medicine, Shenzhen, Guangdong, People's Republic of China.
authors.forename: Duo
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Medicine/GI Division, Johns Hopkins University and Sidney Kimmel Cancer Center, Baltimore, MD, USA.
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Meltzer
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D020022:Genetic Predisposition to Disease; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D010641:Phenotype; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: bioinformatics; gastric cancer; miRNA profiling