title: Activation of farnesoid X receptor downregulates monocyte chemoattractant protein-1 in murine macrophage.
abstract: Farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily, which plays important roles in bile acids/lipid homeostasis and inflammation. Monocyte chemoattractant protein-1 (MCP-1) contributes to macrophage infiltration into body tissues during inflammation. Here we investigated whether FXR can regulate MCP-1 expression in murine macrophage. FXR activation down regulate MCP-1 mRNA and protein levels in ANA-1 and Raw264.7 cells. Luciferase reporter assay, Gel shift and Chromatin immunoprecipitation assays have revealed that the activated FXR bind to the FXR element located in -738 bp ∼  -723 bp in MCP-1 promoter. These results suggested that FXR may serve as a novel target for regulating MCP-1 levels for the inflammation related diseases therapies.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Liangpeng
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Jiahe
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Chanjui
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Third Military Medical University, 400038, Chongqing, China. Electronic address: yujiang0207@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line / Q000187:drug effects; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D002635:Chenodeoxycholic Acid / Q000494:pharmacology; D015536:Down-Regulation; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011401:Promoter Regions, Genetic; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D000099150:Receptor, Farnesoid X-Activated
chemical_list: D018932:Chemokine CCL2; D002635:Chenodeoxycholic Acid; D018160:Receptors, Cytoplasmic and Nuclear; D000099150:Receptor, Farnesoid X-Activated; C491768:Ccl2 protein, mouse
keywords: Bile acids; Farnesoid X receptor; Inflammation; Macrophage; Monocyte chemoattractant protein-1