title: Identification of the Polyketide Biosynthetic Machinery for the Indolizidine Alkaloid Cyclizidine.
abstract: The cyclizidine biosynthetic gene cluster was identified from Streptomyces NCIB 11649, which revealed the polyketide biosynthetic machinery for cyclizidine alkaloid biosynthesis. Both in vivo mutagenesis study and in vitro biochemical analysis provided insight into the timing and mechanism of the biosynthetic enzymes that produce cyclizidine-type indolizidine alkaloids.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Berkeley, California 94720, United States.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Berkeley, California 94720, United States.
authors.forename: Seong Jong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Berkeley, California 94720, United States.
authors.forename: Joyce
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California , Berkeley, California 94720, United States.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D055808:Drug Discovery; D054836:Indolizidines / Q000737:chemistry*; D015394:Molecular Structure; D005810:Multigene Family; D061065:Polyketides / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000470:Alkaloids; D054836:Indolizidines; D061065:Polyketides; C557937:ent-cyclizidine