title: The relationship of oxidative stress markers and parameters of myocardial function in a rat model of cardiotoxicity.
abstract: Although a majority of studies related oxidative stress to cardiovascular diseases, the pathophysiological relevance has been remaining unknown. The aim of this study was to establish the relationship among different commonly used biomarkers of oxidative stress and cardiovascular dys/function in rats. A pathological state in many aspects similar to that of acute myocardial infarction was induced by administration of isoprenaline (100mg.kg(-1), s.c.) in Wistar:Han rats. Haemodynamic, biochemical and ECG parameters were measured in two sets of experiments: after 24hours and continuously during the first 2hours following the administration of isoprenaline. Serum cardiac troponin T (cTnT) correlated strongly with cardiac function, myocardial calcium levels, wet ventricles weight and relevant ECG parameters (T wave, R wave and J - junction - point amplitudes). However, only weak negative correlations were found for cTnT and total blood glutathione or serum vitamin C concentrations, while no significant associations were found with serum vitamin E and plasma TBARS. Although the oxidized form of glutathione correlated positively with heart rate, no correlation with the above-mentioned ECG parameters was found. However, correlations of in 8-isoprostane with both R wave and J-junction-point amplitudes were observed. Conclusively, more selective markers of oxidative stress may predict the functional status of the heart.
authors.affiliation: Charles University in Prague (Faculty of Pharmacy in Hradec Králové), Department of Pharmacology and Toxicology, Czech Republic. Electronic address: mladenkap@faf.cuni.cz.
authors.forename: Premysl
authors.initials: P
authors.lastname: Mladenka
authors.affiliation: Charles University in Prague (Faculty of Pharmacy in Hradec Králové), Department of Pharmacology and Toxicology, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Filipský
authors.affiliation: Charles University in Prague (Faculty of Pharmacy in Hradec Králové), Department of Pharmacology and Toxicology, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Ríha
authors.affiliation: Charles University in Prague (Faculty of Medicine in Hradec Králové), University Hospital Hradec Králové, Czech Republic.
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Vávrová
authors.affiliation: Charles University in Prague (Faculty of Medicine in Hradec Králové), University Hospital Hradec Králové, Czech Republic.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Holecková
authors.affiliation: Charles University in Prague (Faculty of Medicine in Hradec Králové), University Hospital Hradec Králové, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
authors.affiliation: Charles University in Prague (Faculty of Pharmacy in Hradec Králové), Department of Pharmacology and Toxicology, Czech Republic.
authors.forename: Radomír
authors.initials: R
authors.lastname: Hrdina