title: 2D-DIGE-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at Iitate village, Fukushima.
abstract: The present study continues our previous research on investigating the biological effects of low-level gamma radiation in rice at the heavily contaminated Iitate village in Fukushima, by extending the experiments to unraveling the leaf proteome. 14-days-old plants of Japonica rice (Oryza sativa L. cv. Nipponbare) were subjected to gamma radiation level of upto 4 µSv/h, for 72 h. Following exposure, leaf samples were taken from the around 190 µSv/3 d exposed seedling and total proteins were extracted. The gamma irradiated leaf and control leaf (harvested at the start of the experiment) protein lysates were used in a 2-D differential gel electrophoresis (2D-DIGE) experiment using CyDye labeling in order to asses which spots were differentially represented, a novelty of the study. 2D-DIGE analysis revealed 91 spots with significantly different expression between samples (60 positive, 31 negative). MALDI-TOF and TOF/TOF mass spectrometry analyses revealed those as comprising of 59 different proteins (50 up-accumulated, 9 down-accumulated). The identified proteins were subdivided into 10 categories, according to their biological function, which indicated that the majority of the differentially expressed proteins consisted of the general (non-energy) metabolism and stress response categories. Proteome-wide data point to some effects of low-level gamma radiation exposure on the metabolism of rice leaves.
authors.affiliation: a Research Reactor Institute; Kyoto University ; Osaka , Japan.|b Institute of Development; Aging and Cancer; Tohoku University ; Sendai , Japan.
authors.forename: Gohei
authors.initials: G
authors.lastname: Hayashi
authors.affiliation: c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.
authors.forename: Carlo F
authors.initials: CF
authors.lastname: Moro
authors.affiliation: d Department of Biology and Microbiology ; South Dakota State University ; SD USA.
authors.forename: Jai Singh
authors.initials: JS
authors.lastname: Rohila
authors.affiliation: c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.|e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.affiliation: f Environmental Stress Mechanisms Section; Center for Environmental Biology and Ecosystem Studies; National Institute for Environmental Studies ; Tsukuba, Ibaraki , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kubo
authors.affiliation: a Research Reactor Institute; Kyoto University ; Osaka , Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Imanaka
authors.affiliation: g Laboratory of International Epidemiology; Center for International Cooperation; Dokkyo Medical University ; Tochigi , Japan.
authors.forename: Shinzo
authors.initials: S
authors.lastname: Kimura
authors.affiliation: h 1372-7 Kusabana ; Tokyo , Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Ozawa
authors.affiliation: a Research Reactor Institute; Kyoto University ; Osaka , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukutani
authors.affiliation: i Department of Quantam Energy Applications ; Graduate School of Engineering; Hiroshima University ; Higashi-Hiroshima , Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Endo
authors.affiliation: j Office Brain ; Tokyo , Japan.
authors.forename: Katsuki
authors.initials: K
authors.lastname: Ichikawa
authors.affiliation: k Research Laboratory for Biotechnology and Biochemistry (RLABB) ; Kathmandu , Nepal.|l GRADE (Global Research Arch for Developing Education) Academy Pvt. Ltd ; Birgunj , Nepal.
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.affiliation: c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.|e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
authors.affiliation: m School of Pharmacy; Showa University ; Tokyo , Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Hori
authors.affiliation: b Institute of Development; Aging and Cancer; Tohoku University ; Sendai , Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukumoto
authors.affiliation: c Department of Anatomy ; School of Medicine; Showa University ; Tokyo , Japan.|e Global Research Center for Innovative Life Science; School of Pharmacy and Pharmaceutical Sciences; Hoshi University ; Tokyo , Japan.|n Faculty of Health and Sport Sciences & Tsukuba International Academy for Sport Studies (TIAS); University of Tsukuba ; Tsukuba , Ibaraki , Japan.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
mesh_terms: D015536:Down-Regulation / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D005720:Gamma Rays; D007564:Japan; D012275:Oryza / Q000378:metabolism* / Q000528:radiation effects; D018515:Plant Leaves / Q000378:metabolism* / Q000528:radiation effects*; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism* / Q000528:radiation effects; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D005456:Fluorescent Dyes; D010940:Plant Proteins; D020543:Proteome
keywords: 2D-DIGE; Fukushima; proteomics; radioactively contaminated soil; rice seedling