title: Functional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis.
abstract: ATP-binding cassette (ABC)-type ATPases are chemomechanical engines involved in diverse biological pathways. Recent genomic information reveals that ABC ATPase domains/subunits act not only in ABC transporters and structural maintenance of chromosome proteins, but also in iron-sulfur (Fe-S) cluster biogenesis. A novel type of ABC protein, the SufBCD complex, functions in the biosynthesis of nascent Fe-S clusters in almost all Eubacteria and Archaea, as well as eukaryotic chloroplasts. In this study, we determined the first crystal structure of the Escherichia coli SufBCD complex, which exhibits the common architecture of ABC proteins: two ABC ATPase components (SufC) with function-specific components (SufB-SufD protomers). Biochemical and physiological analyses based on this structure provided critical insights into Fe-S cluster assembly and revealed a dynamic conformational change driven by ABC ATPase activity. We propose a molecular mechanism for the biogenesis of the Fe-S cluster in the SufBCD complex.
authors.affiliation: From the Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka 560-0043, Japan, the Organization for Promotion of Tenure Track, University of Miyazaki, Miyazaki 889-1692, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Hirabayashi
authors.affiliation: the Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan.
authors.forename: Eiki
authors.initials: E
authors.lastname: Yuda
authors.affiliation: the Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tanaka
authors.affiliation: the Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
authors.forename: Sumie
authors.initials: S
authors.lastname: Katayama
authors.affiliation: the Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Iwasaki
authors.affiliation: the Rigaku Corporation, Tokyo 196-8666, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: the Institute for Protein Research, Osaka University, Osaka 565-0871, Japan.
authors.forename: Genji
authors.initials: G
authors.lastname: Kurisu
authors.affiliation: the Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina 29208, and.
authors.forename: F Wayne
authors.initials: FW
authors.lastname: Outten
authors.affiliation: the Division of Applied Chemistry, Graduate School of Engineering Osaka University, Osaka 565-0871, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuyama
authors.affiliation: the Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan, ytaka@molbiol.saitama-u.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: the Organization for Promotion of Tenure Track, University of Miyazaki, Miyazaki 889-1692, Japan, keiwada@med.miyazaki-u.ac.jp.
authors.forename: Kei
authors.initials: K
authors.lastname: Wada
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D053838:Scattering, Small Angle; D017386:Sequence Homology, Amino Acid; D014961:X-Ray Diffraction
chemical_list: D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; D007506:Iron-Sulfur Proteins
keywords: ABC ATPase; ABC transporter; ATPase; Suf machinery; conformational change; iron-sulfur protein; x-ray crystallography