title: Natural Killer Cells and Innate Interferon Gamma Participate in the Host Defense against Respiratory Vaccinia Virus Infection.
abstract: In establishing a respiratory infection, vaccinia virus (VACV) initially replicates in airway epithelial cells before spreading to secondary sites of infection, mainly the draining lymph nodes, spleen, gastrointestinal tract, and reproductive organs. We recently reported that interferon gamma (IFN-γ) produced by CD8 T cells ultimately controls this disseminated infection, but the relative contribution of IFN-γ early in infection is unknown. Investigating the role of innate immune cells, we found that the frequency of natural killer (NK) cells in the lung increased dramatically between days 1 and 4 postinfection with VACV. Lung NK cells displayed an activated cell surface phenotype and were the primary source of IFN-γ prior to the arrival of CD8 T cells. In the presence of an intact CD8 T cell compartment, depletion of NK cells resulted in increased lung viral load at the time of peak disease severity but had no effect on eventual viral clearance, disease symptoms, or survival. In sharp contrast, RAG(-/-) mice devoid of T cells failed to control VACV and succumbed to infection despite a marked increase in NK cells in the lung. Supporting an innate immune role for NK cell-derived IFN-γ, we found that NK cell-depleted or IFN-γ-depleted RAG(-/-) mice displayed increased lung VACV titers and dissemination to ovaries and a significantly shorter mean time to death compared to untreated NK cell-competent RAG(-/-) controls. Together, these findings demonstrate a role for IFN-γ in aspects of both the innate and adaptive immune response to VACV and highlight the importance of NK cells in T cell-independent control of VACV in the respiratory tract.
  
  
  OBJECTIVE
  Herein, we provide the first systematic evaluation of natural killer (NK) cell function in the lung after infection with vaccinia virus, a member of the Poxviridae family. The respiratory tract is an important mucosal site for entry of many human pathogens, including poxviruses, but precisely how our immune system defends the lung against these invaders remains unclear. Natural killer cells are a type of cytotoxic lymphocyte and part of our innate immune system. In recent years, NK cells have received increasing levels of attention following the discovery that different tissues contain specific subsets of NK cells with distinctive phenotypes and function. They are abundant in the lung, but their role in defense against respiratory viruses is poorly understood. What this study demonstrates is that NK cells are recruited, activated, and contribute to protection of the lung during a severe respiratory infection with vaccinia virus.
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Georges
authors.initials: G
authors.lastname: Abboud
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Vikas
authors.initials: V
authors.lastname: Tahiliani
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Pritesh
authors.initials: P
authors.lastname: Desai
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Varkoly
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: John
authors.initials: J
authors.lastname: Driver
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA.
authors.forename: Tarun E
authors.initials: TE
authors.lastname: Hutchinson
authors.affiliation: Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, Florida, USA ssalek@ufl.edu.
authors.forename: Shahram
authors.initials: S
authors.lastname: Salek-Ardakani
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000821:virology; D008212:Lymphocyte Depletion; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010053:Ovary / Q000821:virology; D012141:Respiratory Tract Infections / Q000276:immunology*; D016019:Survival Analysis; D014615:Vaccinia / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology*; D019562:Viral Load
chemical_list: D007371:Interferon-gamma