title: Guanine-rich DNA-based peroxidase mimetics for colorimetric assays of alkaline phosphatase.
abstract: DNA-based peroxidase mimetics are facilely constructed through Cu(II)-coordination with different oligonucleotides involving G20, C20, A20 and T20, respectively, with high peroxidase mimicking activity as well as high stability against proteins. Peroxidase-like activities of DNA-Cu(II) complexes are greatly associated with the sequence composition of DNA templates, which decrease in the following order: G20>C20>A20>T20. G20-Cu(II) complex ([Cu(2+)]/[base]=0.05) possesses the Km value of 0.257 mM toward 3,3',5,5'-tetramethylbenzidine and 102.3mM toward hydrogen peroxide at 25 °C. G20-Cu(II) complexes are employed to develop a colorimetric turn-on assay of alkaline phosphatase with high sensitivity and selectivity, on the basis of pyrophosphate-induced inhibition of their intrinsic peroxidase-like activities. The limit of detection is achieved as 0.84 U/L with the linear response region of 20-200 U/L. Such colorimetric assay system is probably applicable for the quantitative determination of ALP in biological fluids.
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Tianjin 300193, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Gu
authors.affiliation: Key Laboratory for Green Chemical Technology MOE, Tianjin University, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin 300072, People's Republic of China. Electronic address: fuyan@tju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry*; D003124:Colorimetry / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006147:Guanine / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006147:Guanine; D003300:Copper; D004247:DNA; D009195:Peroxidase; D000469:Alkaline Phosphatase
keywords: Alkaline Phosphatase; Colorimetry; DNA; Peroxidase