title: STIM1 is cleaved by calpain.
abstract: Store-operated calcium entry (SOCE) is a pathway that moves Ca(2+) across the plasma membrane and is mediated by two major proteins, STIM1 and Orai1. Here, we discovered that the cytoplasmic domain of STIM1 is a target for calpains, a family of Ca(2+)-activated proteases. We found that calpain cleavage of STIM1 serves to control its cellular abundance and was noticeably increased under conditions of cellular stress and apoptosis. Dysregulation of STIM1 levels has been reported to have human disease consequences and our results suggest a mechanism for controlling STIM1 abundance.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S7, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Prins
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2S7, Canada. Electronic address: marek.michalak@ualberta.ca.
authors.forename: Marek
authors.initials: M
authors.lastname: Michalak
mesh_terms: D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry* / Q000378:metabolism*; D002154:Calpain / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism*; D059748:Proteolysis; D013312:Stress, Physiological; D000071737:Stromal Interaction Molecule 1
chemical_list: D015220:Calcium Channels; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C103530:STIM1 protein, human; C100757:Stim1 protein, mouse; D000071737:Stromal Interaction Molecule 1; D002154:Calpain; D002118:Calcium
keywords: Calpain; Store-operated calcium entry; Stromal interaction molecule 1