title: Synthesis and Functional Reconstitution of Light-Harvesting Complex II into Polymeric Membrane Architectures.
abstract: One of most important processes in nature is the harvesting and dissipation of solar energy with the help of light-harvesting complex II (LHCII). This protein, along with its associated pigments, is the main solar-energy collector in higher plants. We aimed to generate stable, highly controllable, and sustainable polymer-based membrane systems containing LHCII-pigment complexes ready for light harvesting. LHCII was produced by cell-free protein synthesis based on wheat-germ extract, and the successful integration of LHCII and its pigments into different membrane architectures was monitored. The unidirectionality of LHCII insertion was investigated by protease digestion assays. Fluorescence measurements indicated chlorophyll integration in the presence of LHCII in spherical as well as planar bilayer architectures. Surface plasmon enhanced fluorescence spectroscopy (SPFS) was used to reveal energy transfer from chlorophyll b to chlorophyll a, which indicates native folding of the LHCII proteins.
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna (Austria).
authors.forename: Thomas
authors.initials: T
authors.lastname: Zapf
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna (Austria).
authors.forename: Cherng-Wen Darren
authors.initials: CW
authors.lastname: Tan
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna (Austria).
authors.forename: Tobias
authors.initials: T
authors.lastname: Reinelt
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna (Austria).
authors.forename: Christoph
authors.initials: C
authors.lastname: Huber
authors.affiliation: CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Keling Road 88, 215163 Suzhou (China).
authors.forename: Ding
authors.initials: D
authors.lastname: Shaohua
authors.affiliation: School of Biological Science, Nanyang Technological University, 60 Nanyang Drive, 637551 Singapore (Singapore).
authors.forename: Susana
authors.initials: S
authors.lastname: Geifman-Shochat
authors.affiliation: Institute of General Botany, Johannes Gutenberg University Mainz, Johannes-von-Müller-Weg 6, 55128 Mainz (Germany).
authors.forename: Harald
authors.initials: H
authors.lastname: Paulsen
authors.affiliation: Department for Nanobiotechnology, Institute of Synthetic Bioarchitectures, University of Natural Resources and Life Science, Muthgasse 11/2, 1190 Vienna (Austria). eva.sinner@boku.ac.at.
authors.forename: Eva-Kathrin
authors.initials: EK
authors.lastname: Sinner
mesh_terms: D002474:Cell-Free System; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D045342:Light-Harvesting Protein Complexes / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D045342:Light-Harvesting Protein Complexes; D008051:Lipid Bilayers; D011108:Polymers; D002734:Chlorophyll; D010447:Peptide Hydrolases
keywords: light-harvesting complexes; membrane models; membrane proteins; protein expression; synthetic polymer membranes