title: Reconstitution and clinical significance of T cell subsets in the early stage after related HLA-mismatched peripheral blood hematopoietic SCT without T-cell depletion in vitro.
abstract: Related HLA-haploidentical HSCT has been applied more and more recently, but the reconstitution of T lymphocyte subsets and its clinical significance in patients received related HLA-haploidentical non T-cell depleted in vitro high-dose peripheral blood hematopoietic SCT (RHNT-PSCT) are incompletely defined. In the present study of our RHNT-PSCT, we found that in non-aGVHD group, CD3(+) T lymphocyte recovered to normal levels gradually between 60 and 90 days, and the recovery of CD4(+) T lymphocyte was retarded significantly, CD4(+)/CD8(+) ratio was apparently inverted. Whereas, the ratio of CD4(+) CD25(+) Foxp3(+) Treg cells was significantly lower in aGVHD group than in healthy control group and non-aGVHD group, and also in grade III-IV aGVHD patients than in grade I-II aGVHD patients. Meanwhile, we observed the level of interleukin-10 (IL-10) gradually increased in serum of patients without aGVHD, but decreased in III-IV aGVHD patients significantly. Spearman correlation analysis showed that serum IL-10 level was negatively correlated with the grade of aGVHD. These results suggest that the reconstitution of peripheral blood T lymphocyte subsets is good, and dynamic detection of Treg cells and serum IL-10 level might predict aGVHD in the early stage after our RHNT-PSCT.
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Pang
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Duan
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qu
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Jianli
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Haizhou
authors.initials: H
authors.lastname: Cao
authors.affiliation: Institute of Hematology, Hematologic Disease Center, First Affiliated Hospital of Xinjiang Medical University Xinjiang Uygur Autonomous Region Research, Urumqi 830011, Xinjiang Uygur Autonomous Region, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D055815:Young Adult
chemical_list: D006680:HLA Antigens
keywords: Allo-HSCT; HLA-mismatched; acute GVHD; lymphocyte subsets; regulatory T-cell