title: Streptomonospora tuzyakensis sp. nov., a halophilic actinomycete isolated from saline soil.
abstract: A novel actinobacterium, designated strain BN506(T), was isolated from soil collected from Tuz (Salt) Lake, Konya, Turkey, and was characterised to determine its taxonomic position. The isolate was found to have morphological and chemotaxonomic properties associated with members of the genus Streptomonospora. The isolate was found to grow optimally at 37 °C and in the presence of 10 % (w/v) NaCl but not in the absence of NaCl. Phylogenetic analyses based on an almost-complete 16S rRNA gene sequences indicated that isolate is closely related to members of the genus Streptomonospora and forms a distinct phyletic line in the Streptomonospora phylogenetic tree. Strain BN506(T) is closely related to Streptomonospora halophila YIM 91355(T) (98.1 % sequence similarity). Sequence similarities with other type strains of the genus Streptomyces were lower than 98.0 %. The cell wall of the novel strain was found to contain meso-diaminopimelic acid. Whole cell hydrolysates were found to contain galactose, glucose and ribose. The predominant menaquinone was identified as MK-10(H8) (57.0 %). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and 10 methyl C18:0. Based on 16S rRNA gene sequence analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain BN506(T) was identified as a member of a novel species of the genus Streptomonospora, for which the name Streptomonospora tuzyakensis sp. nov. (type strain BN506(T) = DSM 45930(T) = KCTC 29210(T)) is proposed.
authors.affiliation: Biology Department, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Tatar
authors.affiliation: Biology Department, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Molecular Biology and Genetics Department, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: Inan
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Biology Department, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Belduz
authors.affiliation: Biology Department, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey. nsahin@omu.edu.tr.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014421:Turkey
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid
keywords: Nocardiopsaceae; Polyphasic taxonomy; Streptomonospora tuzyakensis