title: Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite-tussah silk fibroin nanoparticles for bone tissue engineering.
abstract: The bone is a composite of inorganic and organic materials and possesses a complex hierarchical architecture consisting of mineralized fibrils formed by collagen molecules and coated with oriented hydroxyapatite. To regenerate bone tissue, it is necessary to provide a scaffold that mimics the architecture of the extracellular matrix in native bone. Here, we describe one such scaffold, a nanostructured composite with a core made of a composite of hydroxyapatite and tussah silk fibroin. The core is encased in a shell of tussah silk fibroin. The composite fibers were fabricated by coaxial electrospinning using green water solvent and were characterized using different techniques. In comparison to nanofibers of pure tussah silk, composite notably improved mechanical properties, with 90-fold and 2-fold higher initial modulus and breaking stress, respectively, obtained. Osteoblast-like MG-63 cells were cultivated on the composite to assess its suitability as a scaffold for bone tissue engineering. We found that the fiber scaffold supported cell adhesion and proliferation and functionally promoted alkaline phosphatase and mineral deposition relevant for biomineralization. In addition, the composite were more biocompatible than pure tussah silk fibroin or cover slip. Thus, the nanostructured composite has excellent biomimetic and mechanical properties and is a potential biocompatible scaffold for bone tissue engineering.
authors.affiliation: Key Laboratory of Advanced Textile Composites, Ministry of Education, Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Shao
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China. Electronic address: hejianxin771117@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Acquired Immune Deficiency Syndrome Treatment and Research Center, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450007, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sang
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ding
authors.affiliation: Key Laboratory of Advanced Textile Composites, Ministry of Education, Institute of Textile Composites, Tianjin Polytechnic University, Tianjin 300387, China. Electronic address: chenli@tjpu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Cui
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
authors.forename: Kejing
authors.initials: K
authors.lastname: Li
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
authors.forename: Qiming
authors.initials: Q
authors.lastname: Han
authors.affiliation: College of Textiles, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Tan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D012831:Bombyx / Q000378:metabolism; D001842:Bone and Bones / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005349:Fibroins; D017886:Durapatite
keywords: Bone tissue engineering; Coaxial electrospinning; Hydroxyapatite; Tussah silk fibroin