title: Ribosome profiling reveals the what, when, where and how of protein synthesis.
abstract: Ribosome profiling, which involves the deep sequencing of ribosome-protected mRNA fragments, is a powerful tool for globally monitoring translation in vivo. The method has facilitated discovery of the regulation of gene expression underlying diverse and complex biological processes, of important aspects of the mechanism of protein synthesis, and even of new proteins, by providing a systematic approach for experimental annotation of coding regions. Here, we introduce the methodology of ribosome profiling and discuss examples in which this approach has been a key factor in guiding biological discovery, including its prominent role in identifying thousands of novel translated short open reading frames and alternative translation products.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.|Center for RNA Systems Biology, University of California, Berkeley, California 94720, USA.|California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, California 94158, USA.
authors.forename: Gloria A
authors.initials: GA
authors.lastname: Brar
authors.affiliation: Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94158, USA.|Center for RNA Systems Biology, University of California, Berkeley, California 94720, USA.|California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, California 94158, USA.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Weissman
mesh_terms: D001483:Base Sequence; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D014176:Protein Biosynthesis / Q000235:genetics*; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D011506:Proteins; D012333:RNA, Messenger