title: Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability.
abstract: OBJECTIVE
  To evaluate the degree of conversion (DC%), water sorption (WS), solubility (SO) and the resin-dentin bonding stability (μTBS) of experimental (EXP) etch-and-rinse adhesive systems containing MMP-inhibitors: Galardin-GAL, Batimastat-BAT, GM1489-GM1 and chlorhexidine diacetate-CHX.
  
  
  METHODS
  DC% was measured using FT-IR spectroscopy, while WS and SO were calculated based on ISO4049. Thirty-six human molars were wet ground until the occlusal dentin was exposed. The adhesive systems were applied and resin composite buildups were incrementally constructed. After 24 h immersion in distilled water at 37 °C, the specimens were cut into resin-dentin beams with a cross-sectional area of 1 mm(2). The μTBS was evaluated after 24 h, 6 months and 12 months of water storage at 37 °C. Adper Single Bond 2 (SB2) was used as a commercial control. The data were analyzed using ANOVA and Tukey's HSD test.
  
  
  RESULTS
  SB2 presented the highest DC% (p<0.05). CHX presented the lowest WS (p<0.05). GM1, GAL and BAT presented similar WS when compared with SB2 (p>0.05). SO was found to be not significant (p>0.05). All adhesive systems maintained μTBS stability after 6 months of water storage. Only BAT, GM1 and CHX maintained μTBs stability after 12 months of water storage.
  
  
  CONCLUSIONS
  The experimental adhesive systems with GM1489 and chlorhexidine diacetate presented the best physicochemical properties and preserved resin-dentin bonding stability after 12 months of water storage.
  
  
  CONCLUSIONS
  GM1489 could be suitable for inclusion as an MMP-inhibitor in etch-and-rinse adhesive systems to maintain resin-dentin bonding stability over time.
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: emsilva@vm.uff.br.
authors.forename: Eduardo Moreira
authors.initials: EM
authors.lastname: da Silva
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: ullmann_carol@hotmail.com.
authors.forename: Carolina Ullmann Fernandes
authors.initials: CU
authors.lastname: de Sá Rodrigues
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: marcosmatosuff@gmail.com.
authors.forename: Marcos Paulo
authors.initials: MP
authors.lastname: de Oliveira Matos
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: thaisrodrigues.90@hotmail.com.
authors.forename: Thais Rodrigues
authors.initials: TR
authors.lastname: de Carvalho
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: gbotelho@vm.uff.br.
authors.forename: Glauco Botelho
authors.initials: GB
authors.lastname: dos Santos
authors.affiliation: Analytical Laboratory of Restorative Biomaterials, School of Dentistry, Federal Fluminense University, Niterói, Rio de Janeiro, Brazil. Electronic address: camaral@vm.uff.br.
authors.forename: Cristiane Mariote
authors.initials: CM
authors.lastname: Amaral
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D008689:Methacrylates / Q000737:chemistry; D008963:Molar; D019279:Resin Cements / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; D019279:Resin Cements; D012117:Resins, Synthetic; D014867:Water; D002710:Chlorhexidine
keywords: Adhesive system; Degree of conversion; Dentin-bonding stability; MMP-inhibitors; Solubility; Sorption