title: Antifreeze and cryoprotective activities of ice-binding collagen peptides from pig skin.
abstract: A novel "hyperactive" ice-binding peptide from porcine collagen was prepared by alkaline protease hydrolysis and a series of column chromatography separations, and then its antifreeze and cryoprotective properties were reported. Using differential scanning calorimetry (DSC), the thermal hysteresis (TH) of ice-binding collagen peptides was closely related to their concentration and crystal fraction. Collagen hydrolysates with maximal TH were obtained by hydrolysis at pH 8.0, DH 15.0%, and 5% alkaline protease at 55°C. After purification by column chromatography, the AP-3 ice-binding collagen peptide (GLLGPLGPRGLL) with 1162.8Da molecular weights exhibited the highest TH (5.28°C), which can be classified as "hyperactive". Recrystallisation and melt-resistance of ice cream were improved by AP-3 ice-binding collagen peptide at 0.2% (w/v) in a similar manner to natural antifreeze proteins. Moreover, the addition of AP-3 collagen peptides in ice cream greatly elevated the glass transition temperature (Tg) to -17.64°C.
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China.
authors.forename: Yu Bing
authors.initials: YB
authors.lastname: Zhu
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China. Electronic address: xufei8135@126.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China.
authors.forename: Jing Song
authors.initials: JS
authors.lastname: Yu
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, P.O. Box 454, No. 516, Jungong Road, Shanghai 200093, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D003094:Collagen / Q000737:chemistry*; D003451:Cryoprotective Agents / Q000737:chemistry*; D010450:Endopeptidases / Q000737:chemistry; D006868:Hydrolysis; D007053:Ice; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D012867:Skin / Q000737:chemistry*; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D003451:Cryoprotective Agents; D007053:Ice; D010455:Peptides; D003094:Collagen; D010450:Endopeptidases; C087822:alkaline protease
keywords: Antifreeze protein; Crystallisation inhibition activity; Differential scanning calorimetry; Glass transition temperature; Ice-binding collagen peptides; Thermal hysteresis activity