title: Upregulated KLK10 inhibits esophageal cancer proliferation and enhances cisplatin sensitivity in vitro.
abstract: The kallikrein-related peptidase 10 (KLK10) gene has tumor-suppressive function in various types of human cancer. However, previous studies showed that KLK10 also acts as an oncogene and is upregulated in gastrointestinal tumors. The role of KLK10 in human esophageal cancer (EC) remains unclear. In the present study, the expression of KLK10 in human esophageal and non-esophageal cancer tissues was investigated by immunohistochemistry. Quantitative RT-PCR and western blot analysis were utilized to detect KLK10 mRNA and protein expression in human esophageal cancer cell lines (TE-1 and Eca-109). Small interference RNA was utilized to specifically knockdown KLK10 expression in Eca-109 and TE-1 cells. Cell proliferation, cell cycle analysis as well as CDDP-dependent apoptosis were determined using a CCK-8 assay and flow cytometry. The results showed that, KLK10 was positive in 67 out of 83 (80.72%) human EC and positive in 3 out of 11 (27.27%) normal tissues (P=0.001). The present study indicated that KLK10 potentially plays a crucial role in Eca-109 cell growth. Additionally, the downregulation of KLK10 induced S-phase arrest and promoted cisplatin-induced apoptosis. The resutls of the present study suggested that KLK10 is a promising novel marker for the diagnostic and therapeutic target of esophageal cancer.
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Fan
authors.affiliation: Department of Thoracic Surgery, Anshan City Cancer Hospital, Anshan, Liaoning 114000, P.R. China.
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Meng
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Luo
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Lv
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Mao
authors.affiliation: College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000201:enzymology*; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; C100936:KLK10 protein, human; D007610:Kallikreins; D002945:Cisplatin