title: Selective and Sensitive Sensing of Flame Retardant Chemicals Through Phage Display Discovered Recognition Peptide.
abstract: We report a highly selective and sensitive biosensor for the detection of an environmentally toxic molecule, decabrominated diphenyl ether (DBDE), one of the most common congeners of the polybrominated frame retardants (polybrominated diphenyl ether (PBDE)), using newly discovered DBDE peptide receptors integrated with carbon nanotube field-effect transistors (CNT-FET). The specific DBDE peptide receptor was identified using a high-throughput screening process of phage library display. The resulting binding peptide carries an interesting consensus binding pocket with two Trp-His/Asn-Trp repeats, which binds to the DBDE in a multivalent manner. We integrated the novel DBDE binding peptide onto the CNT-FET using polydiacetylene coating materials linked through cysteine-maleimide click chemistry. The resulting biosensor could detect the desired DBDE selectively with a 1 fM detection limit. Our combined approaches of selective receptor discovery, material nanocoating through click chemistry, and integration onto a sensitive CNT-FET electronic sensor for desired target chemicals will pave the way toward the rapid development of portable and easy-to-use biosensors for desired chemicals to protect our health and environment.
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Hyo-Eon
authors.initials: HE
authors.lastname: Jin
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Chris
authors.initials: C
authors.lastname: Zueger
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Chung
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Winnie
authors.initials: W
authors.lastname: Wong
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.|Department of Mechanical Engineering, Korea University , Seoul 136-713, Korea.
authors.forename: Byung Yang
authors.initials: BY
authors.lastname: Lee
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Seung-Wuk
authors.initials: SW
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques; D061505:Cell Surface Display Techniques; D057930:Click Chemistry; D055768:Halogenated Diphenyl Ethers / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D018000:Receptors, Peptide / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D055768:Halogenated Diphenyl Ethers; D037742:Nanotubes, Carbon; D010455:Peptides; D018000:Receptors, Peptide; C086401:pentabromodiphenyl ether
keywords: Phage display; biosensor; environmental toxicant; polybrominated diphenyl ether