title: Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine.
abstract: An immunochromatographic strip was developed for the serological detection of pseudorabies virus (PRV) in swine. In the strip, the expressed protein of gB, one of the glycoproteins of PRV, labeled with colloidal gold, was used as the detector; staphylococcal protein A and swine anti-pseudorabies virus antibody were blotted on nitrocellulose membrane for the test and control lines, respectively. The specificity of the strip was 98.1%, and the sensitivity of the strip with reference anti-PRV serum was 96.0%. Swine serum samples (296) were collected to evaluate the characteristics of the strip in comparison with an existing commercial kit. The agreement was 93.6%. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents, or equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Xiujie
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Yaning
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang) yang-suzhen@163.com zhanggaiping2003@163.com.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Yinbiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Qianyue
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Xuewu
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang).
authors.forename: Chengliu
authors.initials: C
authors.lastname: Guo
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang) yang-suzhen@163.com zhanggaiping2003@163.com.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary*; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification*; D011557:Pseudorabies / Q000175:diagnosis*; D011934:Reagent Strips / Q000737:chemistry; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
chemical_list: D000914:Antibodies, Viral; D011934:Reagent Strips
keywords: Enzyme-linked immunosorbent assay; immunochromatographic strip; pseudorabies virus; swine