title: Soybean Hydrophobic Protein is Present in a Matrix Secreted by the Endocarp Epidermis during Seed Development.
abstract: Hydrophobic protein from soybean (HPS) is present in soybean dust and is an allergen (Gly m 1) that causes asthma in allergic individuals. Past studies have shown that HPS occurs on the seed surface. To determine the microscopic localization of HPS during seed development, monoclonal antibodies to HPS were used to visualize the protein by fluorescence and transmission electron microscopy. Seed coat and endocarp sections were also examined for pectin, cellulose, callose, starch, and protein by histochemical staining. HPS is present in the endocarp epidermal cells at 18 to 28 days post anthesis. At later stages of seed development, HPS occurs in extracellular secretions that accumulate unevenly on the endocarp epidermis and seed surface. HPS is synthesized by the endocarp epidermis and deposited on the seed surface as part of a heterogeneous matrix.
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, N2L 3G1, Canada.
authors.forename: Daryl E
authors.initials: DE
authors.lastname: Enstone
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, N2L 3G1, Canada.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Peterson
authors.affiliation: Agriculture and Agri-Food Canada, London, N5V 4T3, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Gijzen
mesh_terms: D000485:Allergens / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002482:Cellulose / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005936:Glucans / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010368:Pectins / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013213:Starch / Q000378:metabolism
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D005936:Glucans; C051630:HPS protein, soybean; D010940:Plant Proteins; D010368:Pectins; D002482:Cellulose; D013213:Starch; C048306:callose