title: Magnetic Enzymatic Platform for Organophosphate Pesticide Detection Using Boron-doped Diamond Electrodes.
abstract: A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10(-9) to 10(-5) M. The estimated limits of detection based on IC10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10(-10) M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.
authors.affiliation: Nanobioelectronics & Biosensors Group, ICN2 - Institut Català de Nanociencia i Nanotecnologia (CERCA-CSIC-UAB).
authors.forename: Flavio
authors.initials: F
authors.lastname: Pino
authors.forename: Tribidasari A
authors.initials: TA
authors.lastname: Ivandini
authors.forename: Kazuya
authors.initials: K
authors.lastname: Nakata
authors.forename: Akira
authors.initials: A
authors.lastname: Fujishima
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Einaga
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000378:metabolism; D001895:Boron / Q000737:chemistry*; D004390:Chlorpyrifos / Q000032:analysis*; D002800:Cholinesterase Inhibitors / Q000032:analysis; D018130:Diamond / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D008863:Microspheres; D019809:Streptavidin / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D014867:Water; D001710:Biotin; D018130:Diamond; D019809:Streptavidin; D000110:Acetylcholinesterase; D004390:Chlorpyrifos; D001895:Boron