title: Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC.
abstract: BACKGROUND
  Corydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC.
  
  
  METHODS
  Xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats were used to evaluate the anti-inflammatory activity of CB. The macrophage binding with high-performance liquid chromatography (HPLC) analysis and HPLC-MS were established to screen the potential active compounds. ELISA kits were performed to measure the levels of IL-6, IL-10, TNF-α and NO in RAW 264.7 macrophages culture media.
  
  
  RESULTS
  The alkaloid extract of CB could inhibit significantly xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats. Two components binded to RAW 264.7 cell were identified as 12-hydroxycorynoline and corynoline. Bioassays demonstrated that these two compounds significantly inhibited LPS-induced IL-6, IL-10, TNF-α and NO levels.
  
  
  CONCLUSIONS
  The results suggest that corynoline and 12-hydroxycorynoline contribute to the anti-inflammatory effects of the alkaloid extract of CB. Our findings suggest that these two compounds can be used as candidate for anti-inflammatory drugs.
authors.affiliation: Post-doctoral Research Center, Nanjing University of Chinese Medicine & Jumpcan Pharmaceuticl Co.,Ltd, Jiangsu, Taizhou, 225441, P. R. China. 13961098521@163.com.|Nanjing University of Chinese Medicine, Jiangsu, Nanjing, 210046, P. R. China. 13961098521@163.com.
authors.forename: Zi-Bo
authors.initials: ZB
authors.lastname: Dong
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu, Nanjing, 210046, P. R. China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu, Nanjing, 210046, P. R. China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Zhao
authors.affiliation: Jumpcan Pharmaceuticl Co., Ltd, Jiangsu, Taizhou, 225441, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Jumpcan Pharmaceuticl Co., Ltd, Jiangsu, Taizhou, 225441, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Tian
authors.affiliation: Jumpcan Pharmaceuticl Co., Ltd, Jiangsu, Taizhou, 225441, P. R. China.
authors.forename: Ben
authors.initials: B
authors.lastname: Niu
authors.affiliation: Jumpcan Pharmaceuticl Co., Ltd, Jiangsu, Taizhou, 225441, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Qi
authors.affiliation: Post-doctoral Research Center, Nanjing University of Chinese Medicine & Jumpcan Pharmaceuticl Co.,Ltd, Jiangsu, Taizhou, 225441, P. R. China. wenmoxiushi@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.affiliation: Jumpcan Pharmaceuticl Co., Ltd, Jiangsu, Taizhou, 225441, P. R. China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Shao
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000032:analysis* / Q000494:pharmacology; D002351:Carrageenan / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031311:Corydalis / Q000737:chemistry*; D004487:Edema / Q000188:drug therapy*; D016753:Interleukin-10; D015850:Interleukin-6; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D013058:Mass Spectrometry; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide; D000067996:RAW 264.7 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000893:Anti-Inflammatory Agents; D001600:Berberine Alkaloids; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C487644:corynoline; D009569:Nitric Oxide; D002351:Carrageenan