title: Systems pharmacology-based dissection of mechanisms of Chinese medicinal formula Bufei Yishen as an effective treatment for chronic obstructive pulmonary disease.
abstract: The present work adopted a systems pharmacology-based approach to provide new insights into the active compounds and therapeutic targets of Bufei Yishen formula (BYF) for the treatment of chronic obstructive pulmonary disease (COPD). In addition, we established a rat model of cigarette smoke- and bacterial infection-induced COPD to validate the mechanisms of BYF action that were predicted in systems pharmacology study. The systems pharmacology model derived 216 active compounds from BYF and 195 potential targets related to various diseases. The compound-target network showed that each herbal drug in the BYF formula acted on similar targets, suggesting potential synergistic effects among these herbal drugs. The ClueGo assay, a Cytoscape plugin, revealed that most targets were related to activation of MAP kinase and matrix metalloproteinases. By using target-diseases network analysis, we found that BYF had great potential to treatment of multiple diseases, such as respiratory tract diseases, immune system, and cardiovascular diseases. Furthermore, we found that BYF had the ability to prevent COPD and its comorbidities, such as ventricular hypertrophy, in vivo. Moreover, BYF inhibited the inflammatory cytokine, and hypertrophic factors expression, protease-antiprotease imbalance and the collagen deposition, which may be the underlying mechanisms of action of BYF.
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.|Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
authors.forename: Yange
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.|Center of Bioinformatics, Northwest A &F University, Yangling, Shaanxi 712100, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D017380:Hypertrophy, Right Ventricular / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D009206:Myocardium / Q000473:pathology / Q000648:ultrastructure; D008517:Phytotherapy; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism
chemical_list: C574914:Bu-Fei-Yi-Shen; D016207:Cytokines; D004365:Drugs, Chinese Herbal; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9