title: Use of TD-GC-TOF-MS to assess volatile composition during post-harvest storage in seven accessions of rocket salad (Eruca sativa).
abstract: An important step in breeding for nutritionally enhanced varieties is determining the effects of the post-harvest supply chain on phytochemicals and the changes in VOCs produced over time. TD-GC-TOF-MS was used and a technique for the extraction of VOCs from the headspace using portable tubes is described. Forty-two compounds were detected; 39 were identified by comparison to NIST libraries. Thirty-five compounds had not been previously reported in Eruca sativa. Seven accessions were assessed for changes in headspace VOCs over 7days. Relative amounts of VOCs across 3 time points were significantly different - isothiocyanate-containing molecules being abundant on 'Day 0'. Each accession showed differences in proportions/types of volatiles produced on each day. PCA revealed a separation of VOC profiles according to the day of sampling. Changes in VOC profiles over time could provide a tool for assessment of shelf life.
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading, Berkshire RG6 6AP, UK. Electronic address: luke.bell@pgr.reading.ac.uk.
authors.forename: Luke
authors.initials: L
authors.lastname: Bell
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, PO Box 915, Cardiff, CF10 3TL, UK.
authors.forename: Natasha D
authors.initials: ND
authors.lastname: Spadafora
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, PO Box 915, Cardiff, CF10 3TL, UK.
authors.forename: Carsten T
authors.initials: CT
authors.lastname: Müller
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading, Berkshire RG6 6AP, UK; Centre for Food Security, University of Reading, Whiteknights, Reading, Berkshire RG6 6AP, UK.
authors.forename: Carol
authors.initials: C
authors.lastname: Wagstaff
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Main Building, PO Box 915, Cardiff, CF10 3TL, UK.
authors.forename: Hilary J
authors.initials: HJ
authors.lastname: Rogers
mesh_terms: D019607:Brassicaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry
keywords: (E)-2-pentenal (PubChem CID: 5364752); (E)-4-oxohex-2-enal (PubChem CID: 6365145); (E,E)-2,4-hexadienal (PubChem CID: 637564); 1-Isothiocyanato-3-methylbutane (PubChem CID: 79086); 1-Penten-3-ol (PubChem CID: 12020); 1-Penten-3-one (PubChem CID: 15394); 2-Methyl-2-butenal (PubChem CID: 5321950); Brassicaceae; Diplotaxis tenuifolia; Eruca sativa; Gas chromatography mass spectrometry; Hexyl-isothiocyanate (PubChem CID: 78120); Iberverin (PubChem CID: 62351); Isothiocyanates; Pentyl isothiocyanate (PubChem CID: 69415); Rocket salad; Rucola; Volatile organic chemicals