title: Centella asiatica Leaf Extract Protects Against Indomethacin-Induced Gastric Mucosal Injury in Rats.
abstract: The present study evaluated the protective effect of Centella asiatica (gotu kola) leaf extract (CAE) against indomethacin (IND)-induced gastric mucosal injury in rats. Gastric mucosal injury was induced by the oral administration of IND to the rats after a 24 h fast. CAE (50 or 250 mg/kg) or lansoprazole (a reference drug) was orally administrated 30 min before the IND administration, and 5 h later, the stomachs were removed to quantify the lesions. Orally administered CAE significantly reduced IND-induced gastric injury. The histopathological observations (hematoxylin-eosin and Periodic acid-Schiff staining) confirmed the protection against gastric mucosal injury. Also, CAE decreased the malondialdehyde content compared to the control group. Moreover, pretreatment with CAE resulted in a significant reduction in the elevated expression of tumor necrosis factor, Cyclooxygenase (COX)-2, and inducible nitric oxide synthase. These results suggested that CAE possesses gastroprotective effects against IND-induced gastric mucosal injury, which could be attributed to its ability to inhibit lipid peroxidation and stimulate gastric mucus secretion in the rat gastric mucosa.
authors.affiliation: 1 Department of New Drug Development, College of Medicine, Inha University Hospital , Incheon, Korea.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zheng
authors.affiliation: 1 Department of New Drug Development, College of Medicine, Inha University Hospital , Incheon, Korea.
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Choi
authors.affiliation: 2 National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital , Incheon, Korea.
authors.forename: Jae Min
authors.initials: JM
authors.lastname: Kim
authors.affiliation: 3 R&D Center , Dongkook Pharmaceutical, Seoul, Korea.
authors.forename: Kyung Hoi
authors.initials: KH
authors.lastname: Cha
authors.affiliation: 3 R&D Center , Dongkook Pharmaceutical, Seoul, Korea.
authors.forename: Kye Wan
authors.initials: KW
authors.lastname: Lee
authors.affiliation: 3 R&D Center , Dongkook Pharmaceutical, Seoul, Korea.
authors.forename: Yu Hwa
authors.initials: YH
authors.lastname: Park
authors.affiliation: 1 Department of New Drug Development, College of Medicine, Inha University Hospital , Incheon, Korea.|2 National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital , Incheon, Korea.
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Hong
authors.affiliation: 1 Department of New Drug Development, College of Medicine, Inha University Hospital , Incheon, Korea.|2 National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorder, Inha University Hospital , Incheon, Korea.|4 Division of Gastroenterology and Hepatology, Inha University Hospital , Incheon, Korea.
authors.forename: Don Haeng
authors.initials: DH
authors.lastname: Lee
mesh_terms: D000818:Animals; D028041:Centella / Q000737:chemistry*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000008:administration & dosage*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D051546:Cyclooxygenase 2; D007213:Indomethacin
keywords: Centella asiatica leaf extract; gastroprotection; indomethacin; lipid peroxidation