title: Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche.
abstract: Despite advances in our understanding of interactions between mouse hematopoietic stem cells (HSCs) and their niche, little is known about communication between human HSCs and the microenvironment. Using a xenotransplantation model and intravital imaging, we demonstrate that human HSCs display distinct motile behaviors to their hematopoietic progenitor cell (HPC) counterparts, and the same pattern can be found between mouse HSCs and HPCs. HSCs become significantly less motile after transplantation, while progenitor cells remain motile. We show that human HSCs take longer to find their niche than previously expected and suggest that the niche be defined as the position where HSCs stop moving. Intravital imaging is the only technique to determine where in the bone marrow stem cells stop moving, and future analyses should focus on the environment surrounding the HSC at this point.
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
authors.forename: Katie
authors.initials: K
authors.lastname: Foster
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
authors.forename: François
authors.initials: F
authors.lastname: Lassailly
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
authors.forename: Fernando
authors.initials: F
authors.lastname: Anjos-Afonso
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
authors.forename: Erin
authors.initials: E
authors.lastname: Currie
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
authors.forename: Kevin
authors.initials: K
authors.lastname: Rouault-Pierre
authors.affiliation: Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK. Electronic address: dominique.bonnet@crick.ac.uk.
authors.forename: Dominique
authors.initials: D
authors.lastname: Bonnet
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000502:physiology*; D055153:Stem Cell Niche