title: A transcriptional reference map of defence hormone responses in potato.
abstract: Phytohormones are involved in diverse aspects of plant life including the regulation of plant growth, development and reproduction, as well as governing biotic and abiotic stress responses. We have generated a comprehensive transcriptional reference map of the early potato responses to exogenous application of the defence hormones abscisic acid, brassinolides (applied as epibrassinolide), ethylene (applied as the ethylene precursor aminocyclopropanecarboxylic acid), salicylic acid and jasmonic acid (applied as methyl jasmonate). Of the 39000 predicted genes on the microarray, a total of 2677 and 2473 genes were significantly differentially expressed at 1 h and 6 h after hormone treatment, respectively. Specific marker genes newly identified for the early hormone responses in potato include: a homeodomain 20 transcription factor (DMG400000248) for abscisic acid; a SAUR gene (DMG400016561) induced in epibrassinolide treated plants; an osmotin gene (DMG400003057) specifically enhanced by aminocyclopropanecarboxylic acid; a gene weakly similar to AtWRKY40 (DMG402007388) that was induced by salicylic acid; and a jasmonate ZIM-domain protein 1 (DMG400002930) which was specifically activated by methyl jasmonate. An online database has been set up to query the expression patterns of potato genes represented on the microarray that can also incorporate future microarray or RNAseq-based expression studies.
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Lea
authors.initials: L
authors.lastname: Wiesel
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Jayne L
authors.initials: JL
authors.lastname: Davis
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Linda
authors.initials: L
authors.lastname: Milne
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Redondo Fernandez
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Miriam B
authors.initials: MB
authors.lastname: Herold
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Jill
authors.initials: J
authors.lastname: Middlefell Williams
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Jenny
authors.initials: J
authors.lastname: Morris
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Pete E
authors.initials: PE
authors.lastname: Hedley
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Brian
authors.initials: B
authors.lastname: Harrower
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Adrian C
authors.initials: AC
authors.lastname: Newton
authors.affiliation: The Division of Plant Sciences, College of Life Science, University of Dundee at the James Hutton Institute, Dundee, UK.
authors.forename: Paul R J
authors.initials: PRJ
authors.lastname: Birch
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Eleanor M
authors.initials: EM
authors.lastname: Gilroy
authors.affiliation: The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Ingo
authors.initials: I
authors.lastname: Hein
mesh_terms: D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D058977:Molecular Sequence Annotation; D010937:Plant Growth Regulators / Q000494:pharmacology*; D015203:Reproducibility of Results; D011198:Solanum tuberosum / Q000187:drug effects* / Q000235:genetics*; D059467:Transcriptome
chemical_list: D010937:Plant Growth Regulators