title: Endometrial cancer cells can express fibrinogen: Immunohistochemistry and RT-PCR analysis.
abstract: We investigated whether endometrial cancer (EC) cells can express fibrinogen. Consecutive patients treated for EC were enrolled (cases). A control group of women who had hysterectomy for benign conditions was identified in a case:control ratio of 4:1. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were performed to identify the presence of fibrinogen and the mRNA of its three chains (α, β, γ) in the tissue specimens from both cases and controls. Sixteen EC cases and 4 benign controls were included. Immunohistochemistry failed in one case of EC. In 12/15 (80%) cases versus 0 controls, a moderate-to-intense positivity for fibrinogen was observed (p = 0.09; OR: 32.1; 95%CI: 1.4-752.9). Six (37.5%) women among the cases versus 0 controls expressed RNA for at least one chain of fibrinogen (p = 0.25). All the cases (6/6, 100%) with positive RT-PCR had moderate-to-intense positive immunohistochemistry. Molecular and immunohistochemistry show that some cases of EC have the capability to express fibrinogen and the mRNA of at least one of its chains.
authors.affiliation: a Department of Obstetrics and Gynecology , University of Insubria, Del Ponte Hospital , Varese , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Uccella
authors.affiliation: a Department of Obstetrics and Gynecology , University of Insubria, Del Ponte Hospital , Varese , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cromi
authors.affiliation: b Department of Surgical and Morphological Sciences , University of Insubria , Varese , Italy.
authors.forename: D
authors.initials: D
authors.lastname: Vigetti
authors.affiliation: c Department of Pathology , University of Insubria , Varese , Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cimetti
authors.affiliation: b Department of Surgical and Morphological Sciences , University of Insubria , Varese , Italy.
authors.forename: S
authors.initials: S
authors.lastname: Deleonibus
authors.affiliation: a Department of Obstetrics and Gynecology , University of Insubria, Del Ponte Hospital , Varese , Italy.
authors.forename: J
authors.initials: J
authors.lastname: Casarin
authors.affiliation: b Department of Surgical and Morphological Sciences , University of Insubria , Varese , Italy.
authors.forename: A
authors.initials: A
authors.lastname: Passi
authors.affiliation: c Department of Pathology , University of Insubria , Varese , Italy.
authors.forename: C
authors.initials: C
authors.lastname: Riva
authors.affiliation: a Department of Obstetrics and Gynecology , University of Insubria, Del Ponte Hospital , Varese , Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ghezzi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005340:Fibrinogen
keywords: Endometrial cancer; Fibrinogen; Immunohistochemistry; Molecular analysis; RT-PCR; Translational research