title: Biomimetic Nanotubes Based on Cyclodextrins for Ion-Channel Applications.
abstract: Biomimetic membrane channels offer a great potential for fundamental studies and applications. Here, we report the fabrication and characterization of short cyclodextrin nanotubes, their insertion into membranes, and cytotoxicity assay. Mass spectrometry and high-resolution transmission electron microscopy were used to confirm the synthesis pathway leading to the formation of short nanotubes and to describe their structural parameters in terms of length, diameter, and number of cyclodextrins. Our results show the control of the number of cyclodextrins threaded on the polyrotaxane leading to nanotube synthesis. Structural parameters obtained by electron microscopy are consistent with the distribution of the number of cyclodextrins evaluated by mass spectrometry from the initial polymer distribution. An electrophysiological study at single molecule level demonstrates the ion channel formation into lipid bilayers, and the energy penalty for the entry of ions into the confined nanotube. In the presence of nanotubes, the cell physiology is not altered.
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Hajar
authors.initials: H
authors.lastname: Mamad-Hemouch
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Hassen
authors.initials: H
authors.lastname: Ramoul
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abou Taha
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bacri
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Huin
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Przybylski
authors.affiliation: Université de Cergy Pontoise, LAMBE, UMR CNRS 8587 , 2 avenue A. Chauvin, 95302 Cergy Pontoise, France.
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Oukhaled
authors.affiliation: Université de Cergy Pontoise, LAMBE, UMR CNRS 8587 , 2 avenue A. Chauvin, 95302 Cergy Pontoise, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Thiébot
authors.affiliation: LPN CNRS, UPR20 , 91460 Marcoussis, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Patriarche
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Jarroux
authors.affiliation: Université d'Evry-Val-d'Essonne, LAMBE, UMR CNRS 8587 , bd F. Mitterrand, 91025 Evry, France.
authors.forename: Juan
authors.initials: J
authors.lastname: Pelta
mesh_terms: D032701:Biomimetics; D003505:Cyclodextrins / Q000737:chemistry; D007473:Ion Channels / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D036103:Nanotechnology; D043942:Nanotubes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D007473:Ion Channels; D008051:Lipid Bilayers; D011108:Polymers
keywords: Cyclodextrin nanotubes; biomimetic ion channels; cytotoxicity assays; ion transport; lipid membrane; single molecule