title: Identifying RBP Targets with RIP-seq.
abstract: Throughout their lifetime RNA molecules interact with a variety of RNA-binding proteins (RBPs). RBPs control gene expression by regulating splicing, polyadenylation, editing, transport, stability, and translation of RNA. There are ~1500 RBPs encoded by the human genome and recent studies have detected ~1100 proteins directly interacting with polyadenylated RNA. Identifying the RNAs bound by RBPs will continue to provide important insights into the regulation of gene expression.
authors.affiliation: Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany.
authors.forename: Hans-Herman
authors.initials: HH
authors.lastname: Wessels
authors.affiliation: Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Hirsekorn
authors.affiliation: Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Ohler
authors.affiliation: Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125, Berlin, Germany. neelanjan.mukherjee@mdc-berlin.de.
authors.forename: Neelanjan
authors.initials: N
authors.lastname: Mukherjee
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012313:RNA
keywords: Immunoprecipitation; RNA-binding protein; RNA-seq; Ribonucleoprotein