title: Early downregulation of acute phase proteins after doxorubicin exposition in patients with breast cancer.
abstract: Chemotherapy remains the first-choice option for adjuvant therapy in breast cancer. Here, we investigated the impact of the first chemotherapic cycle of doxorubicin on the plasmatic-proteomic profiling of women diagnosed with breast cancer (n = 87). Blood samples were obtained from the same patient before and after doxorubicin infusion (1 h, 60 mg/m(2)) and processed for label-free LC-MS proteomic screening. A total of 80 proteins were downregulated after chemotherapy. In silico analysis revealed that the main biological process enrolled was inflammation and canonical pathways involving acute phase proteins. TNF-α, IL-1β, IL-12, TGF-β1, clusterin, and gelsolin were chosen as relevant for further validation. All selected targets presented reduced plasmatic levels after treatment. Our results indicate that doxorubicin downregulated acute phase proteins immediately after its infusion. Since such proteins are cancer promoting, its downregulation could support the effectiveness of doxorubicin along treatment.
authors.affiliation: Laboratório de Células Tronco, Instituto Nacional do Câncer, INCA, Rio de Janeiro, Brazil. carolpanis@sercomtel.com.br.|Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, UNIOESTE, Francisco Beltrão, Paraná, Brazil. carolpanis@sercomtel.com.br.
authors.forename: Carolina
authors.initials: C
authors.lastname: Panis
authors.affiliation: Laboratório de Células Tronco, Instituto Nacional do Câncer, INCA, Rio de Janeiro, Brazil.|Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil.
authors.forename: Luciana
authors.initials: L
authors.lastname: Pizzatti
authors.affiliation: Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, UNIOESTE, Francisco Beltrão, Paraná, Brazil.
authors.forename: Aedra Carla
authors.initials: AC
authors.lastname: Bufalo
authors.affiliation: Pontifícia Universidade Católica, PUC, Londrina, Paraná, Brazil.
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Herrera
authors.affiliation: Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, UNIOESTE, Francisco Beltrão, Paraná, Brazil.|Faculdade de Medicina, Universidade de São Paulo, USP, São Paulo, Brazil.
authors.forename: Vanessa Jacob
authors.initials: VJ
authors.lastname: Victorino
authors.affiliation: Laboratório de Fisiopatologia de Radicais Livres, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Cecchini
authors.affiliation: Laboratório de Células Tronco, Instituto Nacional do Câncer, INCA, Rio de Janeiro, Brazil.|INCT para o Controle do Câncer, Rio de Janeiro, Brazil.
authors.forename: Eliana
authors.initials: E
authors.lastname: Abdelhay
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000627:therapeutic use; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D002853:Chromatography, Liquid; D003198:Computer Simulation; D015536:Down-Regulation; D004317:Doxorubicin / Q000008:administration & dosage / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D020543:Proteome / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013997:Time Factors
chemical_list: D000209:Acute-Phase Proteins; D000903:Antibiotics, Antineoplastic; D020543:Proteome; D004317:Doxorubicin
keywords: Acute phase proteins; Breast cancer; Cytokines; Downregulation; Doxorubicin; Label-free proteomics