title: Haematococcus pluvialis soluble proteins: Extraction, characterization, concentration/fractionation and emulsifying properties.
abstract: A water-soluble matrix was extracted from green vegetative Haematococcus pluvialis through high-pressure cell disruption either at native pH (5.7) or with pH shifting to neutral (7). The resulting supernatant is mainly composed of carbohydrates and proteins, with the highest yield of proteins obtained at neutral pH (73±2% of total biomass proteins). The key emulsification properties of the proteins isolated in neutral supernatant (emulsification capacity (EC): 534±41mLoilg(-1) protein, emulsification stability (ES): 94±3% and emulsification activity index (EAI): 80±1m(2)g(-1)) were comparable to the native supernatant values (EC: 589±21mLoilg(-1) protein, ES: 84±3% and EAI: 75±1m(2)g(-1)). Confronted to sodium caseinate (EC: 664±30mLoilg(-1) protein, ES: 63±4%, and EAI: 56±4m(2)g(-1)) these results highlighted the strong potential of proteins isolated from H. pluvialis as emulsifier agent. Moreover, experiments have shown that the stability of emulsions obtained from supernatants is due to the proteins rather than the carbohydrates.
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière Cedex, France.
authors.forename: Fatou
authors.initials: F
authors.lastname: Ba
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière Cedex, France.
authors.forename: Alina Violeta
authors.initials: AV
authors.lastname: Ursu
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière Cedex, France. Electronic address: celine.laroche@univ-bpclermont.fr.
authors.forename: Céline
authors.initials: C
authors.lastname: Laroche
authors.affiliation: Clermont Université, ENSCCF, Institut Pascal UMR CNRS 6602, axe GePEB, 4 Avenue Blaise Pascal, TSA 60206, 63178 Aubière, France.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Djelveh
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D005591:Chemical Fractionation; D000460:Chlorophyta / Q000737:chemistry*; D045703:Emulsifying Agents / Q000737:chemistry / Q000494:pharmacology*; D004655:Emulsions / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D045703:Emulsifying Agents; D004655:Emulsions; D010940:Plant Proteins; D014867:Water
keywords: Emulsification; Haematococcus pluvialis; Proteins; Ultrafiltration