title: One step electrodeposition of dendritic gold nanostructures on β-lactoglobulin-functionalized reduced graphene oxide for glucose sensing.
abstract: Dendritic gold nanostructures (AuNDs) were successfully synthesized by one step electrodeposition on reduced graphene oxide (rGO) functionalized by a globular protein, β-lactoglobulin (BLG), for the first time. Owing to its sulfhydryl groups, water-soluble BLG-rGO provided a superb platform for the growth of AuNDs. Scanning electron microscopy, Raman spectroscopy and X-ray spectroscopy analysis were used to investigate the as prepared BLG-rGO-AuNDs nanocomposite. Electrocatalytic ability of the nanocomposite was evaluated by cyclic voltammetry and chronoamperometric method. In order to prove the superiority of BLG-rGO-AuNDs, we developed a novel glucose biosensor on the nanocomposite modified glassy carbon electrode (GCE) through a cross-linking method. The biosensor exhibited a remarkable sensitivity of 46.2 μA mM(-1) cm(-2), a wide linear range of 0.05-6 mM glucose, a low detection limit of 22.9 µM (S/N=3), and a rapid response time (within 6 s). The prepared biosensor also used to detect glucose in human serum and statistical analysis in the respect of reproducibility was done.
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Du
authors.affiliation: College of Life Science, Nankai University, Tianjin 300071, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Miao
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ma
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Weizhen
authors.initials: W
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Bingkai
authors.initials: B
authors.lastname: Han
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Weijin Road No. 94, Tianjin 300071, China. Electronic address: qiangchen@nankai.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004595:Electroplating; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*
chemical_list: D007782:Lactoglobulins; D010087:Oxides; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
keywords: Dendritic gold nanostructures; Electrodeposition; Glucose biosensor; Nanocomposite; β-Lactoglobulin-functionalized reduced graphene oxide