title: Arcticiflavibacter luteus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from intertidal sand.
abstract: A yellow-pigmented, rod-shaped, non-flagellated, aerobic and Gram-reaction-negative bacterium, designated strain SM1212T, was isolated from intertidal sand of Kongsfjorden, Svalbard. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1212T constituted a distinct lineage within the family Flavobacteriaceae. It shared highest 16S rRNA gene sequence similarities with the type strains of Bizionia echini (96.0 %), Lacinutrix jangbogonensis (95.8 %) and Psychroserpens damuponensis (95.7 %) and < 95.6 % sequence similarity with other recognized species in the family Flavobacteriaceae. The strain grew at 4-35 °C and with 0-6.0 % (w/v) NaCl. It hydrolysed gelatin, DNA, starch and Tween 80 but did not reduce nitrate to nitrite. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C15 : 1 G, anteiso-C15 : 1 A, iso-C15 : 0 3-OH, C17 : 0 2-OH and iso-C17 : 0 3-OH and the major respiratory quinone was menaquinone MK-6. Polar lipids included phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminophospholipid, three unidentified aminolipids and nine unidentified lipids. The genomic DNA G+C content of strain SM1212T was 36.6 mol%. On the basis of data from this polyphasic study, strain SM1212T represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Arcticiflavibacter luteus gen. nov., sp. nov. is proposed. The type strain of Arcticiflavibacter luteus is SM1212T ( = MCCC 1K00234T = KCTC 32514T).
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Beijing National Day School, Beijing 100039, PR China.
authors.forename: Xi-Ruo
authors.initials: XR
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
authors.forename: Hai-Nan
authors.initials: HN
authors.lastname: Su
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013538:Svalbard; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride; C042455:menaquinone 6