title: Immobilization of pectin depolymerising polygalacturonase using different polymers.
abstract: Polygalacturonase catalyses the hydrolysis of pectin substances and widely has been used in food and textile industries. In current study, different polymers such as calcium alginate beads, polyacrylamide gel and agar-agar matrix were screened for the immobilization of polygalacturonase through entrapment technique. Polyacrylamide gel was found to be most promising one and gave maximum (89%) immobilization yield as compared to agar-agar (80%) and calcium alginate beads (46%). The polymers increased the reaction time of polygalacturonase and polymers entrapped polygalacturonases showed maximum pectinolytic activity after 10 min of reaction as compared to free polygalacturonase which performed maximum activity after 5.0 min of reaction time. The temperature of polygalacturonase for maximum enzymatic activity was increased from 45°C to 50°C and 55°C when it was immobilized within agar-agar and calcium alginate beads, respectively. The optimum pH (pH 10) of polygalacturonase was remained same when it was immobilized within polyacrylamide gel and calcium alginate beads, but changed from pH 10 to pH 9.0 after entrapment within agar-agar. Thermal stability of polygalacturonase was improved after immobilization and immobilized polygalacturonases showed higher tolerance against different temperatures as compared to free enzyme. Polymers entrapped polygalacturonases showed good reusability and retained more than 80% of their initial activity during 2nd cycles.
authors.affiliation: Department of Chemistry, University of Turbat, Kech, Balochistan, Pakistan. Electronic address: rehman.haneef8@gmail.com.
authors.forename: Haneef
authors.initials: H
authors.lastname: Ur Rehman
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.affiliation: Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, Dir (Upper), KPK, Pakistan.
authors.forename: Muhammad Asif
authors.initials: MA
authors.lastname: Nawaz
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Asad
authors.initials: A
authors.lastname: Karim
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Maria
authors.initials: M
authors.lastname: Ghani
authors.affiliation: Faculty of Agriculture, Lasbela University of Agriculture, Water and Marine Sciences (LUAWMS), Uthal 90150, Balochistan, Pakistan.
authors.forename: Abdul Hameed
authors.initials: AH
authors.lastname: Baloch
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan.
authors.forename: Shah Ali
authors.initials: SA
authors.lastname: Ul Qader
mesh_terms: D000362:Agar / Q000737:chemistry; D000464:Alginates / Q000737:chemistry; D002118:Calcium / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D010368:Pectins / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D006603:Hexuronic Acids; D011108:Polymers; D010368:Pectins; D020723:Glucuronic Acid; D000362:Agar; D011096:Polygalacturonase; D002118:Calcium
keywords: Agar-agar matrix; Calcium alginate beads; Entrapment; Immobilization; Polyacrylamide gel; Polygalacturonase