title: Polyanionic Carboxyethyl Peptide Nucleic Acids (ce-PNAs): Synthesis and DNA Binding.
abstract: New polyanionic modifications of polyamide nucleic acid mimics were obtained. Thymine decamers were synthesized from respective chiral α- and γ-monomers, and their enantiomeric purity was assessed. Here, we present the decamer synthesis, purification and characterization by MALDI-TOF mass spectrometry and an investigation of the hybridization properties of the decamers. We show that the modified γ-S-carboxyethyl-T10 PNA forms a stable triplex with polyadenine DNA.
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia; Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Kirillova
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: Nataliya
authors.initials: N
authors.lastname: Boyarskaya
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: Andrey
authors.initials: A
authors.lastname: Dezhenkov
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia; Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.
authors.forename: Mariya
authors.initials: M
authors.lastname: Tankevich
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Prokhorov
authors.affiliation: Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia; Department of Structure-Functional Analysis of Biopolymers, Engelhardt Institute of Molecular Biology, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Varizhuk
authors.affiliation: Department of Biotechnology and Bionanotechnology, Moscow State University of Fine Chemical Technologies, Moscow, Russia.
authors.forename: Sergei
authors.initials: S
authors.lastname: Eremin
authors.affiliation: Department of Bioorganic Chemistry, Biology Faculty, Moscow State University, Moscow, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Esipov
authors.affiliation: Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.
authors.forename: Igor
authors.initials: I
authors.lastname: Smirnov
authors.affiliation: Department of Molecular Biology and Genetics, SRI of Physical-Chemical Medicine, Moscow, Russia.
authors.forename: Galina
authors.initials: G
authors.lastname: Pozmogova
mesh_terms: D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D009757:Nylons / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*
chemical_list: D004247:DNA; D009757:Nylons; D009841:Oligonucleotides; D010455:Peptides; D000071228:Polyelectrolytes; D011108:Polymers