title: Has-miR-30a regulates autophagic activity in cervical cancer upon hydroxycamptothecin exposure.
abstract: Cervical cancer is a leading cause of morbidity and mortality in women worldwide. Hydroxycamptothecin (HCPT) represents a new generation of antitumor agents targeting DNA topoisomerase I, which has been applied to treat various cancers with fewer side effects. Autophagy is emerging as an important biological mechanism in targeting human cancers, including cervical cancer. In this study, We have reported that HCPT could induce autophagy in Hela cells. Our investigation of the underlying mechanisms revealed that the decreased expression of miR-30a is involved in HCPT-induced autophagy. Futhermore, we showed that miR-30a could directly target a specific fragment in the 3' untranslated region of Beclin-1 as demonstrated by luciferase assay and overexpression of hsa-miR-30a by transfecting with miR-30a mimic could block HCPT-induced autophagic activity. It is the first time provide a deeper understanding of the mechanisms underlying cellular and molecular mechanisms by which HCPT affect cervical cancer.
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China; The Third Renmin Hospital of Xiantao City, Xiaotao, 433000 Hubei, PR China.
authors.forename: Gantao
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
authors.forename: Binshu
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China.
authors.forename: Limei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430062, Hubei, PR China. Electronic address: hongliwuhan@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Hong
mesh_terms: D020413:3' Untranslated Regions; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D001665:Binding Sites; D002166:Camptothecin / Q000494:pharmacology*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014162:Transfection; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; C546086:MIRN30b microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D002166:Camptothecin
keywords: Autophagy; Hydroxycamptothecin; miR-30a