title: Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Using Sol-Gel Based Microarray.
abstract: BACKGROUND
  Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance. In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV).
  
  
  METHODS
  The Hi3-1 assay was tested using four panels (Panel 1, n = 4,581 patient samples; Panel 2, n = 15 seroconversion samples; Panel 3, n = 4 performance samples; and Panel 4, n = 251 purchased positive control samples), and the results were collected by the Department of Laboratory Medicine, Korea University Medical College, Republic of Korea. The present study compares the sensitivity of the multiplex detection platform for both HIV and HCV using a sol-gel based microarray, which was based on a reference test (Architect HIV Ag/Ab Combo and Architect anti-HCV assays), in Korean patients.
  
  
  RESULTS
  The sensitivity of the multiplex detection platform for both HIV and HCV was 100%, and the specificity was 99.96% for HIV and 99.76% for HCV, which is equivalent to that of the reference test.
  
  
  CONCLUSIONS
  We have successfully applied a novel screening technology to multiplex HIV and HCV diagnoses in a blood bank screening test.
authors.affiliation: The Armed Forces Medical School, Daejeon, Republic of Korea ; Department of Laboratory Medicine, Kangwon National University College of Medicine, Chuncheon, Republic of Korea ; Department of Laboratory Medicine, Korea University Medical College, Seoul, Republic of Korea.
authors.forename: Seung Gyu
authors.initials: SG
authors.lastname: Yun
authors.affiliation: Department of Laboratory Medicine, Korea University Medical College, Seoul, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jang
authors.affiliation: Department of Laboratory Medicine, Korea University Medical College, Seoul, Republic of Korea.
authors.forename: Jong Han
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Korea University Medical College, Seoul, Republic of Korea.
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.affiliation: PCL, Inc., Seoul, Republic of Korea.
authors.forename: Jinhong
authors.initials: J
authors.lastname: Kim
authors.affiliation: PCL, Inc., Seoul, Republic of Korea.
authors.forename: Yeona
authors.initials: Y
authors.lastname: Ki
authors.affiliation: PCL, Inc., Seoul, Republic of Korea.
authors.forename: Minjoung
authors.initials: M
authors.lastname: Jo
authors.affiliation: PCL, Inc., Seoul, Republic of Korea ; Lab of Nanobiotechnology, Dongguk University, Seoul, Republic of Korea.
authors.forename: Soyoun
authors.initials: S
authors.lastname: Kim
mesh_terms: D000906:Antibodies / Q000276:immunology*; D001782:Blood Donors; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis / Q000821:virology; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D044367:Phase Transition; D056910:Republic of Korea
chemical_list: D000906:Antibodies