title: Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose.
abstract: 2-Butanone is an important commodity chemical of wide application in different areas. In this study, Klebsiella pneumoniae was engineered to directly produce 2-butanone from glucose by extending its native 2, 3-butanediol synthesis pathway. To identify the potential enzyme for the efficient conversion of 2, 3-butanediol to 2-butanone, we screened different glycerol dehydratases and diol dehydratases. By introducing the diol dehydratase from Lactobacillus brevis and deleting the ldhA gene encoding lactate dehydrogenase, the engineered K. pneumoniae was able to accumulate 246 mg/L of 2-butanone in shake flask. With further optimization of culture condition, the titer of 2-butanone was increased to 450 mg/L. This study lays the basis for developing an efficient biological process for 2-butanone production.
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Tsinghua Innovation Center in Dongguan, Dongguan 523808, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: He
authors.initials: H
authors.lastname: Sun
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Tsinghua Innovation Center in Dongguan, Dongguan 523808, China.
authors.forename: Dehua
authors.initials: D
authors.lastname: Liu
mesh_terms: D053898:Biosynthetic Pathways; D002074:Butanones / Q000378:metabolism*; D003038:Cobamides / Q000378:metabolism; D003681:Dehydration; D004789:Enzyme Activation; D005285:Fermentation; D017353:Gene Deletion; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism*; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods; D011408:Propanediol Dehydratase / Q000235:genetics / Q000378:metabolism
chemical_list: D002074:Butanones; D003038:Cobamides; C005222:methylethyl ketone; D006836:Hydro-Lyases; D011408:Propanediol Dehydratase; C020671:glycerol dehydratase; D005947:Glucose