title: Evaluation of Pregnancy Malaria Vaccine Candidates: The Binding Inhibition Assay.
abstract: The parasite-binding inhibition assay is designed to evaluate the acquisition of naturally acquired functional antibodies that block Plasmodium falciparum binding to endothelial or placental receptors. The assay is also used to assess functional activity by antibodies induced by immunization, for example antibodies raised against pregnancy malaria vaccine candidates like VAR2CSA. Here we describe a plate-based assay to measure the levels of adhesion-blocking antibodies. This assay format can be adapted to any lab that is minimally equipped for short-term parasite culture.
authors.affiliation: Center for Infectious Disease Research, Seattle, WA, 98109, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Saveria
authors.affiliation: Laboratory of Malaria Immunology and Vaccinology, NIAID, NIH, 12735 Twinbrook Parkway, Room 3W15, Rockville, MD, 20852, USA.
authors.forename: Patrick E
authors.initials: PE
authors.lastname: Duffy
authors.affiliation: Laboratory of Malaria Immunology and Vaccinology, NIAID, NIH, 12735 Twinbrook Parkway, Room 3W15, Rockville, MD, 20852, USA. michal.fried@nih.gov.
authors.forename: Michal
authors.initials: M
authors.lastname: Fried
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology* / Q000378:metabolism; D000953:Antigens, Protozoan / Q000276:immunology* / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D005260:Female; D006801:Humans; D017780:Malaria Vaccines / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology / Q000469:parasitology*; D010920:Placenta / Q000469:parasitology; D010963:Plasmodium falciparum / Q000378:metabolism / Q000472:pathogenicity; D011247:Pregnancy
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017780:Malaria Vaccines; C512601:VAR2CSA protein, Plasmodium falciparum
keywords: Binding inhibition assay; CSA; IgG purification; Plate-based assay