title: Colonic Lesions, Cytokine Profiles, and Gut Microbiota in Plasminogen-Deficient Mice.
abstract: Plasminogen-deficient (FVB/NPan-plg(tm1Jld), plg(tm1Jld)) mice, which are widely used as a wound-healing model, are prone to spontaneous rectal prolapses. The aims of this study were 1) to evaluate the fecal microbiome of plg(tm1Jld) mice for features that might contribute to the development of rectal prolapses and colonic inflammation and 2) to assess the relevance of the inflammatory phenotype to the variability in wound healing in this model. The (plgtm1Jld) mice exhibited delayed wound healing, and they could be divided into 3 distinct groups that differed according to the time until wound closure. Colonic lesions in plg(tm1Jld) mice, which were characterized by necrotizing ulcerations and cystically dilated glands, were restricted to the intermediate and distal parts of the colon. The cytokine profile was indicative of chronic tissue damage, but the genetic modification did not change the composition of the gut microbiota, and none of the clinical or biochemical parameters correlated with the gut microbiota composition.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark. billvestergaard@hotmail.com.
authors.forename: Bill
authors.initials: B
authors.lastname: Vestergaard
authors.affiliation: Department of Departments of Food Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Krych
authors.affiliation: Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Leif R
authors.initials: LR
authors.lastname: Lund
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Bettina P
authors.initials: BP
authors.lastname: Jørgensen
authors.affiliation: Department of Biology, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Lars
authors.initials: L
authors.lastname: Hansen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Henrik E
authors.initials: HE
authors.lastname: Jensen
authors.affiliation: Department of Departments of Food Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Dennis S
authors.initials: DS
authors.lastname: Nielsen
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Axel K
authors.initials: AK
authors.lastname: Hansen
mesh_terms: D000818:Animals; D003092:Colitis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome; D020022:Genetic Predisposition to Disease; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D009336:Necrosis; D010641:Phenotype; D010958:Plasminogen / Q000172:deficiency* / Q000235:genetics; D012005:Rectal Prolapse / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D010958:Plasminogen