title: Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche.
abstract: Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. In this study, we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote GSC progeny differentiation. Defective Wnt signaling causes the loss of the differentiation niche and the upregulated BMP signaling in differentiated GSC progeny, thereby disrupting germ cell differentiation. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.|Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, United States.
authors.forename: Su
authors.initials: S
authors.lastname: Wang
authors.affiliation: Center for Life Sciences, College of Life Sciences, School of Medical Sciences, Tsinghua University, Beijing, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Song
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.|Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, United States.
authors.forename: Xing
authors.initials: X
authors.lastname: Ma
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Center for Life Sciences, College of Life Sciences, School of Medical Sciences, Tsinghua University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Center for Life Sciences, College of Life Sciences, School of Medical Sciences, Tsinghua University, Beijing, China.
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Center for Life Sciences, College of Life Sciences, School of Medical Sciences, Tsinghua University, Beijing, China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Ni
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Malanowski
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Perera
authors.initials: P
authors.lastname: Anoja
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Jungeun
authors.initials: J
authors.lastname: Park
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.
authors.forename: Jeff
authors.initials: J
authors.lastname: Haug
authors.affiliation: Stowers Institute for Medical Research, Kansas City, United States.|Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, United States.
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004330:Drosophila; D029721:Drosophila Proteins / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005854:Germ Cells / Q000502:physiology*; D005982:Glutathione Transferase / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D010053:Ovary / Q000166:cytology; D010084:Oxidation-Reduction; D013234:Stem Cells / Q000502:physiology*; D051153:Wnt Proteins / Q000378:metabolism; D060449:Wnt Signaling Pathway; D051154:Wnt2 Protein / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D006023:Glycoproteins; D051153:Wnt Proteins; D051154:Wnt2 Protein; C454442:Wnt4 protein, Drosophila; D005982:Glutathione Transferase
keywords: D. melanogaster; Gst; Wnt signaling; developmental biology; differentiation niche; germ line stem cells; redox; stem cells