title: Comparison of Effects of Pulsed Electromagnetic Field Stimulation on Platelet-Rich Plasma and Bone Marrow Stromal Stem Cell Using Rat Zygomatic Bone Defect Model.
abstract: BACKGROUND
  Reconstruction of bone defects that occur because of certain reasons has an important place in plastic and reconstructive surgery. The objective of the treatments of these defects was to reinstate the continuity of tissues placed in the area in which the defect has occurred. In this experimental study, the effect of pulsed electromagnetic field stimulation on platelet-rich plasma (PRP) and bone marrow stromal cell, which propounded that they have positive impact on bone regeneration, was evaluated with the bone healing rate in the zygomatic bone defect model enwrapped with superficial temporal fascia.
  
  
  METHODS
  After creating a 4-mm defect on the zygomatic bone of the experiments, the defect was encompassed with a superficial temporal fascial flap and a nonunion model was created. After surgery, different combinations of the PRP, bone marrow stromal cell, and electromagnetic field applications were implemented on the defective area. All the experiments were subjected to bone density measurement.
  
  
  RESULTS
  The result revealed that the PRP and pulsed electromagnetic field implementation were rather a beneficial and an effective combination in terms of bone regeneration.
  
  
  CONCLUSIONS
  It was observed that the superficial temporal fascial flap used in the experiment was a good scaffold choice, providing an ideal bone regeneration area because of its autogenous, vascular, and 3-dimensional structures. As a result, it is presumed that this combination in the nonhealing bone defects is a rather useful treatment choice and can be used in a reliable way in clinical applications.
authors.affiliation: From the *Department of Plastic, Reconstructive and Aesthetic Surgery, Adana State Hospital, Adana; †Department of Plastic, Reconstructive and Aesthetic Surgery, Lutfi Kirdar Kartal Education and Training Hospital, Istanbul; Departments of ‡Plastic, Reconstructive and Aesthetic Surgery and Burn Center, §Biophysics, and ∥Biochemistry, Dicle University Medical Faculty, Diyarbakir; ¶Department of Biochemistry, Cizre State Hospital, Sirnak; and #Department of Biostatistics, Dicle University Medical Faculty, Diyarbakir, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Kapi
authors.forename: Mehmet
authors.initials: M
authors.lastname: Bozkurt
authors.forename: Caferi Tayyar
authors.initials: CT
authors.lastname: Selcuk
authors.forename: Mehmet Salih
authors.initials: MS
authors.lastname: Celik
authors.forename: Veysi
authors.initials: V
authors.lastname: Akpolat
authors.forename: Fatma Birgul
authors.initials: FB
authors.lastname: Isik
authors.forename: Beri Hocaoglu
authors.initials: BH
authors.lastname: Bozarslan
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Celik
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D003131:Combined Modality Therapy; D005205:Fascia / Q000637:transplantation*; D055909:Magnetic Field Therapy / Q000379:methods*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps / Q000637:transplantation*; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000502:physiology; D015050:Zygoma / Q000293:injuries* / Q000502:physiology / Q000601:surgery