title: A novel fluorescent reagent for recognition of triplex DNA with high specificity and selectivity.
abstract: A fluorescent agent (DMT) was screened for recognizing triplex DNA with a specific and selective characteristic, which was embedded into the triplex DNA structure. The triplex DNA was firstly formed by a complementary target sequence through two distinct and sequential events. The conditions including pH and hybridization time, fluorescent agent concentration and embedding time were optimized in the experiment. Under the optimum conditions, the fluorescence signal was enhanced up to 9-fold in comparison with the DMT embedding into the ssDNA, dsDNA and G-quadruplexes. Under the same fluorescence conditions, the changes of the fluorescence signal were also investigated by several kinds of base mismatched DNAs in the experiment. The results showed that our biosensor provided excellent discrimination efficiency toward the perfectly mismatched target DNA with no formation of triplex DNA. We preliminarily deduced the mechanism of the fluorescent reagent for recognizing triplex DNA with high specificity and selectivity.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com and Key Laboratory of Applied Organic Chemistry, College of Jiangxi Province Department of Chemistry, Shangrao Normal University, 334001, Jiangxi, China.
authors.forename: Zongbao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com.
authors.forename: Huimi
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Ma
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian 350002, China. zlan@fzu.edu.cn guonanchen@126.com.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; C051802:triplex DNA; D004247:DNA