title: In wild tobacco, Nicotiana attenuata, variation among bacterial communities of isogenic plants is mainly shaped by the local soil microbiota independently of the plants' capacity to produce jasmonic acid.
abstract: The phytohormone jasmonic acid (JA) plays a central role in defense against necrotrophic pathogens and herbivores in Nicotiana attenuata. Recently Santhanam et al.(1) showed that JA does not have a major role in shaping the root- and shoot associated bacterial communities, though a few taxa differed among control (empty vector, EV) plants and plants impaired in their capacity to produce JA (irAOC). In this addendum, we provide additional data showing that the composition of the plant bacterial communities is mainly shaped by tissue type. The qualitative data analysis revealed that at the order level, 5 bacterial OTUs formed a core community found in all tissues irrespective of genotypes, while 9 OTUs were different among roots and shoots. The heterogeneity among individual plants was high masking the potential genotype effect on bacterial communities. Using a culture-dependent approach, 3 of 18 bacterial taxa retrieved either only from one of the genotypes or from both had a growth promoting effect on EV and irAOC seedlings. The data suggest that the local soil niche in which the roots grows is a major driver of the variability in root bacterial communities recruited by different individuals, and the plant growth-promoting effects of some taxa are independent of the genotype.
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology ; Jena, Germany.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Santhanam
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology ; Jena, Germany.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Baldwin
authors.affiliation: Department of Molecular Ecology; Max Planck Institute for Chemical Ecology ; Jena, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Groten
keywords: Nicotiana attenuata; bacterial communities; jasmonic acid; leaf- and root-associated bacteria; opportunistic mutualism; plant growth promotion