title: Ghrelin modulates hypothalamic fatty acid-sensing and control of food intake in rainbow trout.
abstract: There is no information available on fish as far as the possible effects of ghrelin on hypothalamic fatty acid metabolism and the response of fatty acid-sensing systems, which are involved in the control of food intake. Therefore, we assessed in rainbow trout the response of food intake, hypothalamic fatty acid-sensing mechanisms and expression of neuropeptides involved in the control of food intake to the central treatment of ghrelin in the presence or absence of a long-chain fatty acid such as oleate. We observed that the orexigenic actions of ghrelin in rainbow trout are associated with changes in fatty acid metabolism in the hypothalamus and an inhibition of fatty acid-sensing mechanisms, which ultimately lead to changes in the expression of anorexigenic and orexigenic peptides resulting in increased orexigenic potential and food intake. Moreover, the response to increased levels of oleate of hypothalamic fatty acid-sensing systems (activation), expression of neuropeptides (enhanced anorexigenic potential) and food intake (decrease) were counteracted by the simultaneous treatment with ghrelin. These changes provide evidence for the first time in fish of a possible modulatory role of ghrelin on the metabolic regulation by fatty acid of food intake occurring in the hypothalamus.
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Velasco
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Librán-Pérez
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Otero-Rodiño
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: López-Patiño
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Míguez
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain.
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Cerdá-Reverter
authors.affiliation: Laboratorio de Fisioloxía AnimalDepartamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, SpainDepartamento de Fisiología de Peces y BiotecnologíaInstituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Castellón, Spain jsoengas@uvigo.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Soengas
mesh_terms: D000818:Animals; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D005227:Fatty Acids / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D054439:Ghrelin / Q000494:pharmacology*; D006706:Homeostasis / Q000502:physiology; D007031:Hypothalamus / Q000187:drug effects* / Q000502:physiology; D009479:Neuropeptides / Q000032:analysis / Q000235:genetics; D019301:Oleic Acid / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D005227:Fatty Acids; D054439:Ghrelin; D009479:Neuropeptides; D012333:RNA, Messenger; D019301:Oleic Acid
keywords: fatty acid; fish; food intake; ghrelin; hypothalamus; trout