title: Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching.
abstract: Vitellogenin (Vg) is a precursor of major egg storage protein, vitellin (Vt), and plays primary roles in reproduction of oviparous vertebrates and invertebrates. Chrysopa septempunctata Wesmael is an important and common predator of various insect pests. Here, we first cloned C. septempunctata Vg gene, CsVg. The complete CsVg cDNA was 5664 bp, which encodes an 1810-residues protein with a predicted molecular mass of 206.23 kDa. Expression profile revealed that CsVg mRNA first appeared on day 4 after emergence, maximally accumulated on day 10, and then declined gradually. RNAi mediated by injection of dsRNA depleted CsVg transcripts, significantly reduced egg-laying amount, and decreased egg hatching rate, suggesting that CsVg functions through effects on egg production and hatching in C. septempunctata.
authors.affiliation: Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Both authors contributed equally to this work.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Both authors contributed equally to this work.
authors.forename: J
authors.initials: J
authors.lastname: Mao
authors.affiliation: Key Laboratory for Biology of Plant Diseases and Insect Pests, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Zengfr@caas.net.cn.
authors.forename: F
authors.initials: F
authors.lastname: Zeng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D005298:Fertility; D020868:Gene Silencing; D007313:Insecta / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum / Q000502:physiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D014819:Vitellogenins
keywords: Chrysopa septempunctata; RNA interference; function; vitellogenin