title: Adsorption properties of macroporous adsorbent resins for separation of anthocyanins from mulberry.
abstract: In this study, the adsorption/desorption characteristics of mulberry anthocyanins (MA) on five types of macroporous resins (XAD-7HP, AB-8, HP-20, D-101 and X-5) were evaluated, XAD-7HP and AB-8 showed higher adsorption/desorption capacities. On the basis of static adsorption test, XAD-7HP and AB-8 resins were selected for kinetics, isotherms and thermodynamics. The adsorption mechanism indicated that the process was better explained by pseudo-first-order kinetics and the Langmuir isotherm model, and the thermodynamics tests showed that the processes were exothermic, spontaneous and thermodynamically feasible. Dynamic tests were performed on a column packed with XAD-7HP and AB-8, and breakthrough volume was reached at 15 and 14 bed volumes of MA solution, respectively. The purity of the fraction by 40% ethanol elution on XAD-7HP reached 93.6%, from which cyanidin-3-glucoside and cyanidin-3-rutinoside were identified by HPLC-ESI-MS/MS. The method could be used to prepare high purity anthocyanins from mulberry fruits as well as other plants.
authors.affiliation: School of the Environment and Safety, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of Medicine, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of the Environment and Safety, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Cobbina
authors.affiliation: School of the Environment and Safety, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: yangliuqing@ujs.edu.cn.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D000872:Anthocyanins / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D000872:Anthocyanins; D010936:Plant Extracts; D012116:Resins, Plant
keywords: Acetic acid (PubChem CID: 176); Adsorption mechanism; Ammonium sulfate (PubChem CID: 6097028); Cyanidin (PubChem CID: 128861); Cyanidin-3-glucoside (PubChem CID: 12303203); Cyanidin-3-rutinoside (PubChem CID: 29231); Ethanol (PubChem CID: 702); Hexose (PubChem CID: 169005); Identification; Macroporous resin; Methanol (PubChem CID: 887); Mulberry anthocyanins; Muriatic acid (PubChem CID: 313); Rutinose (PubChem CID: 441429); Separation