title: Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with fungal laccase.
abstract: An enzyme-based method for destaining polyacrylamide gels stained with Coomassie Brilliant Blue R-250 is described. Distilled water supplemented with diluted fermentation broth of a laccase-producing white-rot fungus, Cerrena sp., was used for gel destaining, and a clear gel background was obtained in 2 h at 37 °C. Sensitivity of protein detection was 10 ng. The method did not require organic solvents or changing the destaining solution. Due to simultaneous gel destaining and dye decolorization, the colorless destaining solution can be disposed of directly. Laccase destaining of polyacrylamide gels was simple, efficient, and environmentally friendly.
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian 350116, China; Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou, Fujian 350116, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian 350116, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian 350116, China; Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou, Fujian 350116, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ye
authors.affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian 350116, China; Fujian Key Laboratory of Marine Enzyme Engineering, Fuzhou, Fujian 350116, China. Electronic address: ljuan@fzu.edu.cn.
authors.forename: Juan
authors.initials: J
authors.lastname: Lin
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005658:Fungi / Q000201:enzymology; D042845:Laccase / Q000737:chemistry; D012394:Rosaniline Dyes / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D012394:Rosaniline Dyes; C016680:polyacrylamide gels; C024757:Coomassie brilliant blue R; D042845:Laccase
keywords: Coomassie Brilliant Blue; Destaining; Laccase; Polyacrylamide gel electrophoresis