title: Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells.
abstract: BACKGROUND
  During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was measured in hemolysates and not in individual RBCs. We hypothesized that analysis of G6PD activity in individual RBC identifies storage-dependent changes in G6PD function.
  
  
  METHODS
  Seven units of stored leukoreduced RBCs, stored in saline-adenine-glucose-mannitol, were sampled every week up to 6 weeks of storage. G6PD activity was determined with the cytofluorometric method and expressed as mean fluorescent intensity (MFI) per RBC.
  
  
  RESULTS
  During storage, G6PD activity decreased significantly. Mean MFI after 3 days of storage was 27.8 ± 8.8 and gradually decreased significantly to 18.0 ± 8.3 after 42 days.
  
  
  CONCLUSIONS
  G6PD activity decreases during storage of leukoreduced RBCs. Our results may form a new target to improve storage conditions of RBCs and subsequently improve the quality of transfusion products.
authors.affiliation: Laboratory of Experimental Intensive Care and Anesthesia/Intensive Care, Academic Medical Centre.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Peters
authors.affiliation: Department of Blood Cell Research, Sanquin Blood Bank, Amsterdam, the Netherlands.
authors.forename: Robin
authors.initials: R
authors.lastname: van Bruggen
authors.affiliation: Department of Blood Cell Research, Sanquin Blood Bank, Amsterdam, the Netherlands.|Department Product and Process Development, Sanquin Blood Bank, Amsterdam, the Netherlands.
authors.forename: Dirk
authors.initials: D
authors.lastname: de Korte
authors.affiliation: Department of Cell Biology and Histology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: Cornelis J F
authors.initials: CJ
authors.lastname: Van Noorden
authors.affiliation: Laboratory of Experimental Intensive Care and Anesthesia/Intensive Care, Academic Medical Centre.
authors.forename: Alexander P J
authors.initials: AP
authors.lastname: Vlaar
mesh_terms: D001793:Blood Preservation; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013997:Time Factors
chemical_list: D005954:Glucosephosphate Dehydrogenase