title: Fabrication and characterization of nanopore-interfaced nanochannel devices.
abstract: Nanofluidic devices combining nanochannels and nanopores may enable a range of novel applications in the field of single-molecule biosensing and manipulation. Here we combine classic lithographically based fabrication and electron beam milling to construct a device that integrates sealed transverse features, such as nanocavities and nanochannels, with embedded pores vertically intersecting the nanochannels. Using fluorescent microscopy, we demonstrate that DNA molecules can be introduced into the nanochannels and translated transversely across the embedded pore in an extended-conformation without undergoing cross-pore translocation. Upon application of a trans-pore voltage drop, the molecules will undergo cross-pore translocation into an adjoining macroscopic reservoir.
authors.affiliation: Dept. of Physics, McGill University, 3600 Rue University, Montreal QC H3A 2T8, Canada.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Zhang
authors.forename: Walter
authors.initials: W
authors.lastname: Reisner
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry*; D004560:Electricity; D044085:Microfluidics; D058608:Nanopores / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*
chemical_list: D004247:DNA