title: The effect of caffeic acid phenethyl ester (CAPE) on metabolic enzymes including acetylcholinesterase, butyrylcholinesterase, glutathione S-transferase, lactoperoxidase, and carbonic anhydrase isoenzymes I, II, IX, and XII.
abstract: Caffeic acid phenethyl ester (CAPE) is an active component of honeybee propolis extracts. Carbonic anhydrases (CAs, EC 4.2.1.1) are widespread and intensively studied metalloenzymes present in higher vertebrates including humans as many diverse isoforms. Acetylcholinesterase (AChE) is responsible for acetyl choline (ACh) hydrolysis and plays a fundamental role in nerve impulse transmission by terminating the action of the ACh neurotransmitter at cholinergic synapses and neuromuscular junctions. Butyrylcholinesterase (BChE) is another enzyme abundantly present in the liver and released into blood in a soluble form. Lactoperoxidase (LPO) is an enzyme involved in fighting pathogenic microorganisms whereas glutathione S-transferases (GSTs) are dimeric proteins present both in prokaryotic and eukaryotic organisms and involved in cellular detoxification mechanisms. In the present study, the inhibition effect of CAPE on human carbonic anhydrase (hCA) isoforms I, II, IX, and XII, AChE, BChE, LPO, and GST was evaluated. CAPE inhibited these enzymes with Kis in the range between micromolar to picomolar. The best inhibitory effect was observed against AChE and BChE.
authors.affiliation: a Department of Chemistry , Faculty of Science, Ataturk University , Erzurum , Turkey .|b Department of Zoology , College of Science, King Saud University , Riyadh , Saudi Arabia .
authors.forename: İlhami
authors.initials: İ
authors.lastname: Gülçin
authors.affiliation: c Dipartimento Di Chimica Ugo Schiff, Università Degli Studi Di Firenze , Firenze , Italy .
authors.forename: Andrea
authors.initials: A
authors.lastname: Scozzafava
authors.affiliation: c Dipartimento Di Chimica Ugo Schiff, Università Degli Studi Di Firenze , Firenze , Italy .|d Department of Neurofarba , Section of Pharmaceutical and Nutriceutical Sciences, Università Degli Studi Di Firenze , Florence , Italy .
authors.forename: Claudiu T
authors.initials: CT
authors.lastname: Supuran
authors.affiliation: e Central Researching Laboratory, Agri Ibrahim Cecen University , Agri , Turkey , and.
authors.forename: Hulya
authors.initials: H
authors.lastname: Akıncıoğlu
authors.affiliation: a Department of Chemistry , Faculty of Science, Ataturk University , Erzurum , Turkey .
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Koksal
authors.affiliation: f Health Services Vocational School, Igdır University , Igdır , Turkey.
authors.forename: Fikret
authors.initials: F
authors.lastname: Turkan
authors.affiliation: b Department of Zoology , College of Science, King Saud University , Riyadh , Saudi Arabia .
authors.forename: Saleh
authors.initials: S
authors.lastname: Alwasel
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D002091:Butyrylcholinesterase / Q000187:drug effects*; D002109:Caffeic Acids / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000187:drug effects*; D005982:Glutathione Transferase / Q000187:drug effects*; D007527:Isoenzymes / Q000187:drug effects*; D007784:Lactoperoxidase / Q000187:drug effects*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D002109:Caffeic Acids; D007527:Isoenzymes; D007784:Lactoperoxidase; D005982:Glutathione Transferase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002256:Carbonic Anhydrases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol
keywords: Acetylcholinesterase; butyrylcholinesterase; caffeic acid phenethyl ester; carbonic anhydrase; glutathione S-transferase; lactoperoxidase