title: Multiple resistance to ACCase and AHAS-inhibiting herbicides in shortawn foxtail (Alopecurus aequalis Sobol.) from China.
abstract: Shortawn foxtail (Alopecurus aequalis) is a troublesome grass weed infesting winter wheat and oilseed rape productions in China. Fenoxaprop-p-ethyl and mesosulfuron-methyl failed to control shortawn foxtail of AHSX-1 population collected from a wheat field in Shou County, Anhui province. Molecular analyses revealed that Asp2078Gly mutation of ACCase and Trp574Leu mutation of AHAS were present in plants of the AHSX-1 population. The homozygous plants were isolated and cultured until seed maturity. Whole-plant herbicide bioassays were conducted in the greenhouse using the purified seeds of F1 generation. Dose-response experiments showed that the AHSX-1 population has evolved a very high level resistance to fenoxaprop-p-ethyl (RI = 275) and mesosulfuron-methyl (RI = 788). To determine the sensitivity to other herbicides, assays were conducted at the single recommended rate of each herbicide. Based on the results, the AHSX-1 population was considered to be highly resistant to clodinafop-propargyl, pyroxsulam and flucarbazone-sodium, moderately or highly resistant to quizalofop-p-ethyl, clethodim, sethoxydim and pinoxaden, and susceptible to isoproturon and chlorotoluron. This is the first report of Asp2078Gly mutation in shortawn foxtail and the two robust dCAPS markers designed could quickly detect Asp2078 and Trp574 mutations in ACCase and AHAS gene of shortawn foxtail, respectively.
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Wenlei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Weitang
authors.initials: W
authors.lastname: Liu
authors.affiliation: College of Agronomy, Anhui Science and Technology University, Fengyang, Anhui 233100, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Long
authors.initials: L
authors.lastname: Du
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China. Electronic address: wangjx@sdau.edu.cn.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Wang
mesh_terms: D000095:Acetolactate Synthase / Q000235:genetics*; D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D002681:China; D003512:Cyclohexanones / Q000633:toxicity; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000633:toxicity*; D006574:Heterocyclic Compounds, 2-Ring / Q000633:toxicity; D009154:Mutation; D010080:Oxazoles / Q000633:toxicity; D011422:Propionates / Q000633:toxicity; D011725:Pyridines / Q000633:toxicity; D011810:Quinoxalines / Q000633:toxicity; D013453:Sulfonylurea Compounds / Q000633:toxicity
chemical_list: D003512:Cyclohexanones; D006540:Herbicides; D006574:Heterocyclic Compounds, 2-Ring; D010080:Oxazoles; D011422:Propionates; D011725:Pyridines; D011810:Quinoxalines; D013453:Sulfonylurea Compounds; C441919:clethodim; C049437:sethoxydim; C521745:mesosulfuron-methyl; C064835:quizalofop-ethyl; C416661:fenoxaprop ethyl; D000095:Acetolactate Synthase; D000103:Acetyl-CoA Carboxylase; C561036:clodinafop-propargyl; C542519:pinoxaden
keywords: ACCase gene; AHAS gene; Alopecurus aequalis; Multiple resistance; Mutation