title: Replacement of sugars to hydrogen production by Rhodobacter capsulatus using dark fermentation effluent as substrate.
abstract: Hydrogen is a promising alternative for the increased global energy demand since it has high energy density and is a clean fuel. The aim of this work was to evaluate the photo-fermentation by Rhodobacter capsulatus, using the dark fermentation effluent as substrate. Different systems were tested by changing the type of sugar in the dark fermentation, investigating the influence of supplementing DFE with sugar and adding alternate and periodically lactose and glucose throughout the process. The supplementation of the DFE with sugar resulted in higher H2 productivity and the replacement of the sugars repeatedly during the photo-fermentation process was important to maintain the cell culture active. By controlling the residual amount of sugar, bacteria inhibition was avoided; lactic acid, that was toxic to the biomass, was consumed and the metabolic route of butyric acid production was predominant. Under optimum conditions, the H2 productivity reached 208.40mmolH2/Ld in 52h.
authors.affiliation: School of Chemical Engineering, Federal University of Uberlandia, Av. Joao Naves de Avila 2121, Santa Monica 38408-144, Uberlandia/MG, Brazil.
authors.forename: Felipe Thales Moreira
authors.initials: FT
authors.lastname: Silva
authors.affiliation: School of Chemical Engineering, Federal University of Uberlandia, Av. Joao Naves de Avila 2121, Santa Monica 38408-144, Uberlandia/MG, Brazil.
authors.forename: Luiza Rojas
authors.initials: LR
authors.lastname: Moreira
authors.affiliation: School of Chemical Engineering, Federal University of Uberlandia, Av. Joao Naves de Avila 2121, Santa Monica 38408-144, Uberlandia/MG, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: de Souza Ferreira
authors.affiliation: School of Chemical Engineering, Federal University of Uberlandia, Av. Joao Naves de Avila 2121, Santa Monica 38408-144, Uberlandia/MG, Brazil. Electronic address: frxbatista@feq.ufu.br.
authors.forename: Fabiana Regina Xavier
authors.initials: FR
authors.lastname: Batista
authors.affiliation: School of Chemical Engineering, Federal University of Uberlandia, Av. Joao Naves de Avila 2121, Santa Monica 38408-144, Uberlandia/MG, Brazil.
authors.forename: Vicelma Luiz
authors.initials: VL
authors.lastname: Cardoso
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D020148:Butyric Acid / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000378:metabolism*; D062065:Wastewater
chemical_list: D056804:Biofuels; D020148:Butyric Acid; D005947:Glucose; D006859:Hydrogen; D019344:Lactic Acid; D007785:Lactose; D062065:Wastewater
keywords: Dark fermentation; Hybrid system; Hydrogen; Photo-fermentation; Rhodobacter capsulatus