title: Ferrocyanide-Ferricyanide Redox Couple Induced Electrochemiluminescence Amplification of Carbon Dots for Ultrasensitive Sensing of Glutathione.
abstract: Here we report a novel solid-state ECL sensor for ultrasensitive sensing of glutathione (GSH) based on ferrocyanide-ferricyanide redox couple (Fe(CN)6(3-/4-)) induced electrochemiluminescence (ECL) amplification of carbon dots (C-dots). The electropolymerization of C-dots and (11-pyrrolyl-1-yl-undecyl) triethylammonium tetrafluoroborate (A2) enabled immobilization of the hydrophilic C-dots on the surface of glassy carbon electrode (GCE) perfectly, while the excellent conductivity of polypyrrole was exploited to accelerate electron transfer between them. The Fe(CN)6(3-/4-) can expeditiously convert the C-dots and S2O8(2-) to C-dot(•-) and SO4(•-), respectively. High yields of the excited state C-dots (C-dots*) were obtained, and a ∼10-fold ECL amplification was realized. The C-dots* obtained through the recombination of electron-injected and hole-injected processes may be impeded due to the interference of GSH to K2S2O8. Therefore, the constructed sensor for GSH showed a detection limit down to 54.3 nM (S/N = 3) and a wide linear range from 0.1-1.0 μM with a correlation coefficient of 0.997.
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu Province 210094, China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Niu
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu Province 210094, China.
authors.forename: Rong-Hui
authors.initials: RH
authors.lastname: Zhu
authors.affiliation: University of Grenoble Alpes-CNRS, DCM UMR 5250, F-38000 Grenoble, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu Province 210094, China.
authors.forename: Xue-Ji
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Sino-French Laboratory of Biomaterials and Bioanalytical Chemistry, School of Environmental and Biological Engineering, Nanjing University of Science and Technology , Nanjing, Jiangsu Province 210094, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Shan
mesh_terms: D002244:Carbon / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005292:Ferricyanides / Q000737:chemistry*; D005295:Ferrocyanides / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D057230:Limit of Detection; D049449:Luminescence; D010084:Oxidation-Reduction
chemical_list: D005292:Ferricyanides; D005295:Ferrocyanides; C007931:hexacyanoferrate III; D002244:Carbon; C020354:hexacyanoferrate II; D005978:Glutathione