title: Coevolution Pattern and Functional Conservation or Divergence of miR167s and their targets across Diverse Plant Species.
abstract: microRNAs (miRNAs), a class of endogenously produced small non-coding RNAs of 20-21 nt length, processed from precursor miRNAs, regulate many developmental processes by negatively regulating the target genes in both animals and plants. The coevolutionary pattern of a miRNA family and their targets underscores its functional conservation or diversification. The miR167 regulates various aspects of plant development in Arabidopsis by targeting ARF6 and ARF8. The evolutionary conservation or divergence of miR167s and their target genes are poorly understood till now. Here we show the evolutionary relationship among 153 MIR167 genes obtained from 33 diverse plant species. We found that out of the 153 of miR167 sequences retrieved from the "miRBase", 27 have been annotated to be processed from the 3' end, and have diverged distinctively from the other miR167s produced from 5' end. Our analysis reveals that gma-miR167h/i and mdm-miR167a are processed from 3' end and have evolved separately, diverged most resulting in novel targets other than their known ones, and thus led to functional diversification, especially in apple and soybean. We also show that mostly conserved miR167 sequences and their target AUXIN RESPONSE FACTORS (ARFs) have gone through parallel evolution leading to functional diversification among diverse plant species.
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Suvakanta
authors.initials: S
authors.lastname: Barik
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Kumar
authors.affiliation: International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Shabari
authors.initials: S
authors.lastname: Sarkar Das
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Yadav
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Vibhav
authors.initials: V
authors.lastname: Gautam
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Archita
authors.initials: A
authors.lastname: Singh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Sharmila
authors.initials: S
authors.lastname: Singh
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067.
authors.forename: Ananda K
authors.initials: AK
authors.lastname: Sarkar
mesh_terms: D019295:Computational Biology / Q000379:methods; D030561:Databases, Nucleic Acid; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010944:Plants / Q000145:classification / Q000235:genetics*; D034622:RNA Interference; D012322:RNA Precursors; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics*; D015203:Reproducibility of Results; D012984:Software; D064878:Web Browser
chemical_list: D035683:MicroRNAs; D012322:RNA Precursors; D012333:RNA, Messenger; D018749:RNA, Plant