title: Characterization of Candida famata Isolated from Poultry Feces for Possible Probiotic Applications.
abstract: We studied here the yeast content of poultry feces, collected randomly from a French farm located in the north of the country. Thus, 81 yeast colonies were isolated and clustered into 22 distinct groups using the rep-PCR method. A single colony was taken from each group and identified using biochemical (ID 32C system) and molecular (sequencing of the D1/D2 domain of 26S rDNA and ITS1-5.8-ITS2 rDNA region) methods. Both methods led to the identification of Candida famata species. One isolate of C. famata strains, named strain Y5, was further studied for its cytotoxicity, adhesion, and surface properties, hemolytic activity, and its survival in simulated gastric and intestine environments. The data obtained advocate the probiotic potential of this isolate.
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université Lille 1 - Sciences et Technologies, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
authors.forename: Alaa
authors.initials: A
authors.lastname: Al-Seraih
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université d'Artois, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Flahaut
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université Lille 1 - Sciences et Technologies, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
authors.forename: François
authors.initials: F
authors.lastname: Krier
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université Lille 1 - Sciences et Technologies, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Cudennec
authors.affiliation: Laboratoire Régional de Recherche en Agroalimentaire et Biotechnologies: Institut Charles Viollette, Université Lille 1 - Sciences et Technologies, Cité scientifique, (Bat Polytech), Boulevard Paul Langevin, 59655, Villeneuve d'Ascq, France. djamel.drider@univ-lille1.fr.
authors.forename: Djamel
authors.initials: D
authors.lastname: Drider
mesh_terms: D000818:Animals; D002175:Candida / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D005243:Feces / Q000382:microbiology*; D005602:France; D006801:Humans; D011200:Poultry / Q000382:microbiology*; D019936:Probiotics
keywords: Candida famata; Poultry feces; Probiotics; Rep-PCR; Yeasts