title: Recent development of chemical derivatization in LC-MS for biomedical approaches.
abstract: LC-MS/MS is currently the most powerful system in biomedical analysis. At the same time, chemical derivatization is a useful technique to enhance the detection sensitivity of nonionizable or poorly ionizable molecules in LC-MS/MS. Derivatization improves the ionization efficiency, the chromatographic separation and/or the chemical stability. This article presents an overview of the recent development of chemical derivatization reagents and reactions for the quantitative analysis of xenobiotic and endogenous molecules such as pharmaceuticals, amino acids, peptides, proteins, steroids, biomarkers and industrial products by LC-MS.
authors.affiliation: Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai, Miyagi 981-8558, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Ohno
authors.affiliation: Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai, Miyagi 981-8558, Japan.
authors.forename: Kouwa
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000032:analysis* / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010455:Peptides / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D013256:Steroids / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D015262:Xenobiotics / Q000032:analysis / Q000737:chemistry
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins; D013256:Steroids; D015262:Xenobiotics