title: The transient expression of Klf4 and Klf5 during adipogenesis depends on GSK3β activity.
abstract: Adipogenesis is regulated by a complex cascade of transcriptional factors, among them KLF4. This factor was previously shown to be necessary for adipose differentiation. We found that GSK3β activity was required for Klf4 and Klf5 expression during adipogenesis. In addition, retinoic acid inhibited Klf4 and Klf5 expression but not that of Cebpb. Protein synthesis inhibition showed that the transient expression of Klf4, Cebpb and Klf5 during early adipogenesis seemed to require a yet unknown protein for their repression. We also found that Klf4 forced expression in 3T3-F442A cells cultured under non-adipogenic conditions did not induce adipogenesis, nor the expression of Cebpb or Klf5, a Cebpb target gene, showing that KLF4 was not sufficient for adipose differentiation to take place. This would suggest that a more complex combination of molecular pathways not yet understood, is involved during early adipogenesis.
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Carla
authors.initials: C
authors.lastname: Cervantes-Camacho
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Alicia
authors.initials: A
authors.lastname: Beltrán-Langarica
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Ann-Karoll
authors.initials: AK
authors.lastname: Ochoa-Uribe
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Meytha
authors.initials: M
authors.lastname: Marsch-Moreno
authors.affiliation: Instituto de Neurobiología; Universidad Nacional Autónoma de México ; Querétaro, México.
authors.forename: Jorge-Tonatiuh
authors.initials: JT
authors.lastname: Ayala-Sumuano
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Velez-delValle
authors.affiliation: Department of Cell Biology; Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional ; México City, Mexico.
authors.forename: Walid
authors.initials: W
authors.lastname: Kuri-Harcuch
keywords: Cebpb; GSK3; Klf4; Klf5; adipogenesis; differentiation; retinoic acid