title: MADS1, a novel MADS-box protein, is involved in the response of Nicotiana benthamiana to bacterial harpin(Xoo).
abstract: MADS-box transcription factor genes are well known for their role in floral organ and seed development. In this study, a novel MADS-box-containing gene, designated NbMADS1, was isolated from leaves of Nicotiana benthamiana. The full-length cDNA was 666 bp and encoded a putative polypeptide of 221 aa with a mass of 24.3 kDa. To assess the role of NbMADS1 in the defence response to bacterial harpin(Xoo), an elicitor of the hypersensitive response, a loss-of-function experiment was performed in N. benthamiana plants using virus-induced gene silencing. Analyses of electrolyte leakage revealed more extensive cell death in the control plants than in NbMADS1-silenced plants. The NbMADS1-silenced plants showed impaired harpin(Xoo)-induced stomatal closure, decreased harpin(Xoo)-induced production of hydrogen peroxide (H2O2) and nitric oxide (NO) in guard cells, and reduced harpin(Xoo)-induced resistance to Phytophthora nicotianae. The compromised stomatal closure observed in the NbMADS1-silenced plants was inhibited by the application of H2O2 and sodium nitroprusside (an NO donor). Taken together, these results demonstrate that the NbMADS1-H2O2-NO pathway mediates multiple harpin(Xoo)-triggered responses, including stomatal closure, hypersensitive cell death, and defence-related gene expression, suggesting that NbMADS1 plays an important role in regulating the response to harpin(Xoo) in N. benthamiana plants.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei 230036, PR China.
authors.forename: Huajian
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Teng
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China.
authors.forename: Jingang
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China.
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, PR China xbzheng@njau.edu.cn.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zheng
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D016923:Cell Death; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010838:Phytophthora / Q000502:physiology*; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054046:Plant Stomata / Q000502:physiology; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D014974:Xanthomonas / Q000235:genetics / Q000502:physiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018076:DNA, Complementary; D026161:MADS Domain Proteins; D010940:Plant Proteins
keywords: Elicitor; MADS-box; Nicotiana benthamiana; harpin; hypersensitive response; stomatal closure.