title: Antimalarial activity of 80 % methanolic extract of Brassica nigra (L.) Koch. (Brassicaceae) seeds against Plasmodium berghei infection in mice.
abstract: BACKGROUND
  Resistances to currently available drugs and insecticides, significant drug toxicities and costs and lack of vaccines currently complicated the treatment of malaria. A continued search for safe, effective and affordable plant-based antimalarial agents thus becomes crucial and vital in the face of these difficulties. The aim of the study was to evaluate the antimalarial activity of 80 % methanolic extract of the seeds of Brassica nigra against Plasmodium berghei infection in mice.
  
  
  METHODS
  Chloroquine sensitive Plasmodium berghei (ANKA strain) was used to test the antimalarial activity of the extract. In suppressive and prophylactic models, Swiss albino male mice were randomly grouped into five groups of five mice each. Group I mice were treated with the vehicle, group II, III and IV were treated with 100, 200, and 400 mg/kg of the extract, respectively and the last group (V) mice were treated with chloroquine (10 mg/kg). The level of parasitemia, survival time and variation in weight of mice were used to determine the antimalarial activity of the extract.
  
  
  RESULTS
  Chemosuppressive activities produced by the extract of the seeds of Brassica nigra were 21.88, 50.00 (P < 0.01) and 53.13 % (P < 0.01), while the chemoprophylactic activities were 17.42, 21.21 and 53.79 % (P < 0.05) at 100, 200 and 400 mg/kg of the extract, respectively as compared to the negative control. Mice treated with 200 and 400 mg/kg extract were significantly (P < 0.05) lived longer and gained weight as compared to negative control in 4-day suppressive test.
  
  
  CONCLUSIONS
  From this study, it can be concluded that the seed extract of Brassica nigra showed good chemosuppressive and moderate chemoprophylactic activities and the plant may contain biologically active principles which are relevant in the treatment and prophylaxis of malaria, thus supporting further studies of the plant for its active components.
authors.affiliation: Department of Pharmacy, Wollega University, Nekemte, Ethiopia.
authors.forename: Abrham Belachew
authors.initials: AB
authors.lastname: Muluye
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia. meshetie21@gmail.com.
authors.forename: Eshetie
authors.initials: E
authors.lastname: Melese
authors.affiliation: Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
authors.forename: Getnet Mequanint
authors.initials: GM
authors.lastname: Adinew
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D008288:Malaria / Q000188:drug therapy*; D008297:Male; D000432:Methanol; D051379:Mice; D009149:Mustard Plant / Q000737:chemistry*; D018512:Parasitemia / Q000188:drug therapy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D010962:Plasmodium berghei / Q000187:drug effects; D012639:Seeds / Q000737:chemistry
chemical_list: D000962:Antimalarials; D010936:Plant Extracts; D000432:Methanol