title: Rapeseed calcineurin B-like protein CBL4, interacting with CBL-interacting protein kinase CIPK24, modulates salt tolerance in plants.
abstract: Calcium is a ubiquitous intracellular secondary messenger in eukaryotes. Upon stress challenge, cytosolic Ca(2+) fluctuation could be sensed and bound by calcineurin B-like proteins (CBLs), which further regulate a group of Ser/Thr protein kinases called CBL-interacting protein kinases (CIPKs) to relay the signal and induce cellular responses. Although the CBL-CIPK network has been demonstrated to play crucial roles in plant development and responses to various environmental stresses in Arabidopsis, little is known about their function in rapeseed. In the present study, we characterized CBL4 gene from rapeseed. We found that CBL4 is localized at the plasma membrane and it interacted with CIPK24 in both yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. Unlike the orthologs in Arabidopsis, rapeseed CIPK24 did not interact with CBL10. Furthermore, expression of rapeseed CBL4 rescued the salt-sensitive phenotype of sos3-1 mutant and overexpression of rapeseed CBL4 in Arabidopsis showed enhanced tolerance of salt stress than wild-type. Overall, the results clarified the function of CBL4 in rapeseed.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: happywuzhenliu@163.com.
authors.forename: Wu-Zhen
authors.initials: WZ
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: dengmin@nwafu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 994051374@qq.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: yangwl@nwafu.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 513768220@qq.com.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: dhqing@nwafu.edu.cn.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: jiangyq@nwafu.edu.cn.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Jiang
mesh_terms: D000222:Adaptation, Physiological; D029741:Brassica rapa / Q000378:metabolism* / Q000502:physiology; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011485:Protein Binding; D012965:Sodium Chloride; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride
keywords: Brassica napus; CBL; CIPK; Calcium; Salt stress