title: The functions of a cucumber phospholipase D alpha gene (CsPLDα) in growth and tolerance to hyperosmotic stress.
abstract: Plant phospholipase D (PLD), which can hydrolyze membrane phospholipids to produce phosphatidic acid (PA), a secondary signaling molecule, has been proposed to function in diverse plant stress responses. In this research, a qRT-PCR analysis indicated that the expression of a cucumber phospholipase D alpha gene (CsPLDα) was induced by salt and drought stresses in the roots and leaves. To further study the roles of CsPLDα in regulating plant tolerance to salt, polyethylene glycol (PEG) and abscisic acid (ABA) stresses, transgenic tobacco plants constitutively overexpressing CsPLDα were produced. A qRT-PCR analysis showed that the CsPLDα transcript levels were high in transgenic tobacco lines, whereas no expression was found in wild type (WT) tobacco, indicating that CsPLDα was successfully transferred into the tobacco genome and overexpressed. Under normal conditions for 30 d, seeds of transgenic lines germinated neatly, and the seedlings were robust and bigger than WT plants. When treated with different concentrations of NaCl, PEG and ABA, germination rates and seedling sizes of the transgenic lines were significantly greater than WT. In addition, the germination times for transgenic lines were also remarkably shorter. Further studies indicated that transgenic lines had longer primary roots and more biomass accumulation than WT plants. The water loss in transgenic lines was also much lower than in WT. These findings suggest that the CsPLDα overexpression positively regulates plant tolerance to hyperosmotic stresses, and that CsPLDα is involved in the ABA regulation of stomatal closure and the alleviation of ABA inhibition on seed germination and seedling growth.
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Meili
authors.initials: M
authors.lastname: Huang
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Di
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Ji
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), Ministry of Agriculture, Tai'an 271018, PR China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), Ministry of Agriculture, Tai'an 271018, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Gong
authors.affiliation: College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an 271018, PR China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Huanghuai Region), Ministry of Agriculture, Tai'an 271018, PR China. Electronic address: beautyyfj@163.com.
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D018533:Biomass; D018553:Cucumis sativus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D018525:Germination; D009997:Osmotic Pressure; D010739:Phospholipase D / Q000235:genetics / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D054046:Plant Stomata / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D010739:Phospholipase D
keywords: ABA; CsPLDα; Osmotic stress; Salt stress; Transgenic tobacco