title: Comparison of two antifungal susceptibility testing of Candida sp. isolates using agar diffusion method: Neo-sensitabs® tablets and Bio-rad® disks.
abstract: BACKGROUND
  The aim of our study was to evaluate the concordance between the two antifungal susceptibility testing of Candida sp. isolates using agar diffusion method: Neo-Sensitabs(®) tablets and Bio-Rad(®) disks.
  
  
  METHODS
  This is a prospective study conducted in the Laboratory of Parasitology and Mycology of the Mohammed V military teaching hospital from February to August 2012. Upon receiving blood cultures and peripheral sites samples, the identification of Candida isolates performed using routine phenotypic standard tests and the realization of the antifungal susceptibility was carried out on Neo-sensitabs(®) tablets and Bio-Rad(®) disks.
  
  
  RESULTS
  A total of 38 Candida strains were isolated: 15 C. albicans (39%), 13 C. glabrata (34%), 5 C. tropicalis (13%), 4 C. krusei (11%) and 1 C. dubliniensis (3%). There were no significant difference (P>0.05) in susceptibility rate between both methods for all antifungal agents tested except for 5-fluorocytosine. The concordance percentage between two methods was 100% for amphotericin B, 97.4% for fluconazole, 94.7% for voriconazole and 73% for 5-fluorocytosine.
  
  
  CONCLUSIONS
  Both methods are easy to perform, rapid and cost effective. Our results showed the best agreement between the two methods for testing the susceptibility of Candida isolates to amphotericin B, fluconazole and voriconazole while for the 5-fluorocytosine, the concordance rate was low.
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco. Electronic address: uwije2020@yahoo.fr.
authors.forename: J
authors.initials: J
authors.lastname: Uwingabiye
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Iken
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: A G
authors.initials: AG
authors.lastname: Zohoun
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: L
authors.initials: L
authors.lastname: Boumhil
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: Z
authors.initials: Z
authors.lastname: Lemkhente
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: H
authors.initials: H
authors.lastname: Naoui
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: Bouchrik
authors.affiliation: Service de parasitologie-mycologie, faculté de médecine et de pharmacie, université Mohammed V, hôpital militaire d'instruction Mohammed V, Rabat, Morocco.
authors.forename: B
authors.initials: B
authors.lastname: Lmimouni
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D015725:Fluconazole / Q000494:pharmacology; D005437:Flucytosine / Q000494:pharmacology; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011446:Prospective Studies; D013607:Tablets; D065819:Voriconazole / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D013607:Tablets; D000666:Amphotericin B; D015725:Fluconazole; D005437:Flucytosine; D065819:Voriconazole
keywords: Agar diffusion method; Antifongiques; Antifungal agents; Antifungal susceptibility methods; Candida; Concordance; Méthode de diffusion en milieu gélosé; Méthodes de sensibilité aux antifongiques