Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice.
A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide-protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1-200 µg/mL in mouse plasma, spleen and lung samples with correlation coefficients greater than 0.995. The inter-day and intra-day precisions of the method were not more than 6.9%, and the relative recovery ranged from 93.7% to 106.4%. The concentration-time curve of LPPF in plasma following intravenous (i.v.) administration at 40 mg/kg body weight well fitted to a two-compartment model. LPPF rapidly eliminated from plasma according to the short half-lives (t1/2α=2.23 min, t1/2β=39.11 min) and mean retention times (MRT0-t=1.15 h, MRT0-∞=1.39 h). After administration over 5 to 360 min, the concentration of LPPF in spleen homogenate decreased from 7.41 to 3.68 µg/mL; the concentration in lung homogenate decreased from 9.08 to 3.40 µg/mL. On the other hand, the increasing concentration of LPPF fraction with low molecular weight in heart homogenate was observed.
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. minting1323@163.com.
Ting
T
Min
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. qiqijiayuguan@163.com.
Jie
J
Sun
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. yiy86@whpu.edu.cn.|Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China. yiy86@whpu.edu.cn.
Yang
Y
Yi
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. wanghongxunhust@163.com.|Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China. wanghongxunhust@163.com.
Hong-Xun
HX
Wang
Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangzhou 510610, China. hf1311@163.com.
Fei
F
Hang
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. aywlingyun@126.com.
You-Wei
YW
Ai
College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China. wanglimeiyx@163.com.|Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China. wanglimeiyx@163.com.
Li-Mei
LM
Wang
D000818:Animals; D002850:Chromatography, Gel; D005638:Fruit / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D010940:Plant Proteins / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D029631:Sapindaceae / Q000737:chemistry*
D046911:Macromolecular Substances; D010940:Plant Proteins; D011134:Polysaccharides
HPSEC-FD; distribution; longan pulp; pharmacokinetics; polysaccharide-protein complex