Twenty-Four Hour Ex Vivo Culture of Celiac Duodenal Biopsies.
Organ culture is a valuable technique in celiac disease research. It provides the opportunity to examine interactions between different cell types during the disease process without the need for invasive in vivo studies. Biopsies are maintained in an oxygen-rich environment, in contact with, but not submerged in, culture medium. A very straightforward and successful method of organ culture is described here.
Immunology Department, Trinity College Dublin, College Green, Dublin 2, Ireland. cooperse@tcd.ie.
Sarah E J
SE
Cooper
Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland. shwilson@tcd.ie.
Sharon
S
Wilson
Immunology Department, Trinity College Dublin, College Green, Dublin 2, Ireland. con.feighery@tcd.ie.|Immunology Department, St James Hospital, Dublin, Ireland. con.feighery@tcd.ie.
Conleth F
CF
Feighery
D001706:Biopsy; D002446:Celiac Disease / Q000175:diagnosis* / Q000473:pathology; D004386:Duodenum / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques
Biopsy; Celiac disease; Culture; Duodenal; Ex vivo; Prolamins; Small bowel