The protective role of DJ-1 in ultraviolet-induced damage of human skin: DJ-1 levels in the stratum corneum as an indicator of antioxidative defense.
DJ-1 is a multifunctional protein associated with Parkinson's disease and plays a significant role in protecting nerve cells from oxidative stress. DJ-1 is expressed in the skin, although its function there is unknown. In this study, we investigated DJ-1 function in keratinocytes. DJ-1 was induced by H2O2 exposure and UV irradiation in keratinocytes. DJ-1 knockdown with small interfering RNA (siRNA) increased reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release after UVB irradiation, suggesting that DJ-1 reduces ROS and might protect skin cells from UV damage in vitro. To investigate the in vivo role of DJ-1 in the skin, we determined DJ-1 levels in human stratum corneum samples obtained by the tape-stripping method. DJ-1 levels in the stratum corneum (scDJ-1) correlated with total antioxidant capacity. We also examined the effect of scDJ-1 on changes in skin after UVB irradiation. DJ-1 was elevated in SC from the upper arm 1 to 2 weeks after UVB irradiation. One day after UVB irradiation, L* (brightness) and a* (redness) values, indicators of skin color, were altered regardless of scDJ-1 expression. However, these values recovered more quickly in subjects with high scDJ-1 expression than in those with low scDJ-1 expression. These data suggest that DJ-1 in skin plays a significant role in protection against UV radiation and oxidative stress, and that DJ-1 levels in the SC might be an indicator of antioxidative defense against UV-induced damage.
Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan. shiishiwata@fancl.co.jp.
Shioji
S
Ishiwatari
Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan.
Minako
M
Takahashi
Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan.
Chie
C
Yasuda
Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan.
Maho
M
Nakagawa
Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
Yoshiro
Y
Saito
Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan.
Noriko
N
Noguchi
Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan.
Shoko
S
Matsukuma
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D019610:Cytoprotection / Q000502:physiology; D004817:Epidermis / Q000473:pathology / Q000528:radiation effects*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D000071617:Protein Deglycase DJ-1; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012880:Skin Pigmentation; D014466:Ultraviolet Rays / Q000009:adverse effects*; D055815:Young Adult
D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D034741:RNA, Small Interfering; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1
DJ-1; Stratum corneum; Tape stripping; Total antioxidant capacity