Epsilon glutathione transferases possess a unique class-conserved subunit interface motif that directly interacts with glutathione in the active site.
Epsilon class glutathione transferases (GSTs) have been shown to contribute significantly to insecticide resistance. We report a new Epsilon class protein crystal structure from Drosophila melanogaster for the glutathione transferase DmGSTE6. The structure reveals a novel Epsilon clasp motif that is conserved across hundreds of millions of years of evolution of the insect Diptera order. This histidine-serine motif lies in the subunit interface and appears to contribute to quaternary stability as well as directly connecting the two glutathiones in the active sites of this dimeric enzyme.
Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore 138673.
Jantana
J
Wongsantichon
Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore 138673 Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117597.
Robert C
RC
Robinson
Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand albertketterman@yahoo.com.
Albert J
AJ
Ketterman
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001665:Binding Sites; D020134:Catalytic Domain / Q000235:genetics; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology*; D005978:Glutathione / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation
D029721:Drosophila Proteins; C000609410:GSTE6 protein, Drosophila; D005982:Glutathione Transferase; D005978:Glutathione
GSTE6; dimer interface; epsilon crystal structure; insect glutathione transferase; protein structure