Synthesis and mechanical evaluation of Sr-doped calcium-zirconium-silicate (baghdadite) and its impact on osteoblast cell proliferation and ALP activity.
For the first time the successful preparation of Sr doped baghdadite (Ca3-x Sr x ZrSi2O9 x  =  0.1 and 0.75) is shown. Sr-doped as well as pure baghdadite are prepared via a versatile solid-state synthesis and conventional sintering at 1400 °C. XRD measurements and crystal structure refinements reveal that a substitution of Ca atoms with Sr and a high purity (>99%) is achieved. The physical, mechanical, and biological properties of these novel bioceramics are presented in relation to the dopant concentration. Incorporating Sr into the baghdadite crystal caused only minor changes to the grain size and the mechanical properties. The characteristic strength ranges from 145 to 168 MPa and a Weibull modulus of 4.9 to 9.2 is observed. Other mechanical properties like fracture toughness and hardness vary from 1.23  ±  0.07 MPam(0.5) to 1.31  ±  0.12 MPam(0.5) and 7.3  ±  0.6 GPa to 8.0  ±  0.7 GPa, respectively. The in vitro cellular response of human osteoblasts showed an increase in the cell proliferation and a significantly higher alkaline phosphatase (ALP) activity with an increase in the Sr content. From the improved biological properties and the suitable mechanical performance we conclude that this material is a highly promising candidate for bone replacement material and bioactive implant coatings.
Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany.
Thomas C
TC
Schumacher
Alieh
A
Aminian
Eike
E
Volkmann
Hanna
H
Lührs
Dawid
D
Zimnik
Dennis
D
Pede
Werner
W
Wosniok
Laura
L
Treccani
Kurosch
K
Rezwan
D000469:Alkaline Phosphatase / Q000378:metabolism*; D018786:Bone Substitutes / Q000138:chemical synthesis*; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival; D002516:Ceramics / Q000737:chemistry*; D019245:Compressive Strength; D055119:Elastic Modulus; D004789:Enzyme Activation; D006244:Hardness; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D017640:Silicates / Q000737:chemistry*; D013314:Stress, Mechanical; D013324:Strontium / Q000737:chemistry*
D018786:Bone Substitutes; C000592768:Ca(3)ZrSi(2)O(9); D017640:Silicates; D000469:Alkaline Phosphatase; D013324:Strontium