Biological properties of Alsidium corallinum and its potential protective effects against damage caused by potassium bromate in the mouse liver.
In the course of searching for hepatoprotective agents from natural sources, the protective effect of chemical constituents of the marine red alga Alsidium corallinum (A. corallinum) against potassium bromate (KBrO3)-induced liver damage in adult mice was investigated. The in vitro antioxidant and antibacterial properties of A. corallinum were firstly investigated. Then, A. corallinum was tested in vivo for its potential protective effects against damage caused by KBrO3 in mice models divided into four groups: controls, KBrO3, KBrO3 + A. corallinum, and A. corallinum. Our results demonstrated the rich composition of A. corallinum in antioxidant compounds like phenolics, flavonoids, anthocyanins, polysaccharides, chlorophyll and carotenoids. Its antioxidant activity was also confirmed using β-carotene bleaching by linoleic acid assay, reducing sugar test and trolox equivalent antioxidant capacity. The ethanolic extract of A. corallinum also showed good inhibition of the tested bacteria. The coadministration of the red alga associated to the KBrO3 alleviated hepatotoxicity as monitored by the improvement of hepatic oxidative stress biomarkers and plasma biochemical parameters, when compared to the KBrO3-treated mice. These results were confirmed by the improvement of histological and molecular changes. Treatment with A. corallinum prevented liver damage induced by KBrO3, thus protecting the body against free radicals and reducing inflammation and hypercholesterolemia risks.
Laboratory of Pharmacology UR/12 ES-13, Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisia.
Hajer
H
Ben Saad
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, Sfax University, BP3038-1173, Sfax, Tunisia.
Nadia
N
Kharrat
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, Sfax University, BP3038-1173, Sfax, Tunisia.
Najeh
N
Krayem
Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University, Sfax, Tunisia.
Ons
O
Boudawara
Anatomopathology Laboratory, CHU Habib Bourguiba, Sfax University, Sfax, Tunisia.
Tahia
T
Boudawara
Life Sciences Department, Animal Physiology Laboratory, Sfax Faculty of Sciences, Sfax University, BP1171, 3000, Sfax, Tunisia. najiba.zeghal@tunet.tn.
Najiba
N
Zeghal
Life Sciences Department, Animal Physiology Laboratory, Sfax Faculty of Sciences, Sfax University, BP1171, 3000, Sfax, Tunisia.
Ibtissem
I
Ben Amara
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D001959:Bromates / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000461:Rhodophyta / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D015415:Biomarkers; D001959:Bromates; D005419:Flavonoids; D008055:Lipids; D020011:Protective Agents; C019536:potassium bromate
Alsidium corallinum; Antioxidant and antibacterial activities; Gene expression; Genotoxicity; Potassium bromate