Surface modification via strain-promoted click reaction facilitates targeted lentiviral transduction.
As a result of their ability to integrate into the genome of both dividing and non-dividing cells, lentiviruses have emerged as a promising vector for gene delivery. Targeted gene transduction of specific cells and tissues by lentiviral vectors has been a major goal, which has proven difficult to achieve. We report a novel targeting protocol that relies on the chemoselective attachment of cancer specific ligands to unnatural glycans on lentiviral surfaces. This strategy exhibits minimal perturbation on virus physiology and demonstrates remarkable flexibility. It allows for targeting but can be more broadly useful with applications such as vector purification and immunomodulation.
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
Yanjie
Y
Chu
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
Yoon Hyeun
YH
Oum
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA; Institute of Chemical Biology and Drug Discovery, State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA. Electronic address: isaac.carrico@stonybrook.edu.
Isaac S
IS
Carrico
D002460:Cell Line; D057930:Click Chemistry / Q000379:methods*; D005453:Fluorescence; D005818:Genetic Engineering / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000821:virology; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods*; D014161:Transduction, Genetic / Q000379:methods*
Bioorthogonal chemistry; Cancer; Gene therapy; Lentivirus; Metabolic labeling; Sialic acid; Surface engineering; Targeting; Tumor angiogenesis; Unnatural glycans