Soil restoration with organic amendments: linking cellular functionality and ecosystem processes.
A hot topic in recent decades, the application of organic amendments to arid-degraded soils has been shown to benefit microbially-mediated processes. However, despite the importance of soils for global sustainability, a gap has not been addressed yet in soil science: is there any connection between ecosystem-community processes, cellular functionality, and microbial lifestyles (i.e. oligotrophy-copiotrophy) in restored soils? Together with classical ecosystem indicators (fatty-acids, extracellular-enzyme activities, basal respiration), state-of-the-art metaproteomics was applied to fill this gap in a model-restoration experiment initiated 10-years ago by the addition of sewage-sludge and compost. Organic amendment strongly impacted ecosystem processes. Furthermore, the type of material used induced differences in the cellular functionalities through variations in the percentages of proteins involved in translation, transcription, energy production and C-fixation. We conclude that the long-term impact of organic restoration goes beyond ecosystem processes and affects cellular functionalities and phyla-lifestyles coupled with differences in microbial-community structures.
Department of Soil and Water Conservation, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus Universitario de Espinardo, Aptdo. de Correos 164, Espinardo 30100 Murcia, Spain.|Department of Agroforestry Technology and Science and Genetics, School of Advanced Agricultural Engineering, Castilla La Mancha University, Campus Universitario s/n, 02071 Albacete, Spain.
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Bastida
Functional Genomics Center Zurich, ETH Zurich/University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
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Selevsek
Department of Soil and Water Conservation, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus Universitario de Espinardo, Aptdo. de Correos 164, Espinardo 30100 Murcia, Spain.
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Torres
Department of Soil and Water Conservation, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus Universitario de Espinardo, Aptdo. de Correos 164, Espinardo 30100 Murcia, Spain.
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Hernández
Department of Soil and Water Conservation, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, Campus Universitario de Espinardo, Aptdo. de Correos 164, Espinardo 30100 Murcia, Spain.
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García
D017753:Ecosystem; D052918:Environmental Restoration and Remediation; D004798:Enzymes; D040901:Proteomics; D012722:Sewage; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
D004798:Enzymes; D012722:Sewage; D012987:Soil