Kit ligand promotes the transition from primordial to primary follicles after in vitro culture of ovine ovarian tissue.
This study evaluated the effects of kit ligand (KL) on the morphology and development of ovine preantral follicles (fresh control) and after 7 days of in vitro culture in α-Minimal Essential Medium (α-MEM; control medium) or the presence of KL (1, 10, 50, 100 or 200 ng/ml). There was an increase in the percentage of primary follicles at the concentration of 100 ng/ml KL, compared with the fresh control, control medium (α-MEM) and the other KL concentrations. Follicle diameter was significantly higher than the control medium only at concentrations of 50 and 100 ng/ml KL. In conclusion, 100 ng/ml KL promoted the transition from primordial to primary follicles (follicular activation) after in vitro culture of ovine ovarian tissue.
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
A Y P
AY
Cavalcante
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
B B
BB
Gouveia
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
R S
RS
Barberino
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
T L B G
TL
Lins
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
L P
LP
Santos
Nucleus of Biotechnology Applied to Ovarian Follicle Development,Federal University of San Francisco Valley,Petrolina,PE,Brazil.
R J S
RJ
Gonçalves
Institute of Health Sciences,University of International Integration Lusophone African-Brazilian,Acarape,CE,Brazil.
J J H
JJ
Celestino
Universidade Federal do Vale do São Francisco (UNIVASF),Campus de Ciências Agrárias.Colegiado de Medicina Veterinária - Laboratório de Biologia Celular,Citologia e Histologia,Rodovia BR 407,Km 12,Lote 543 - Projeto de Irrigação Nilo Coelho - S/N,C1,CEP: 56300-990,Petrolina,PE,Brasil.
M H T
MH
Matos
D000818:Animals; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D009930:Organic Chemicals / Q000737:chemistry / Q000494:pharmacology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D012756:Sheep; D019089:Stem Cell Factor / Q000494:pharmacology*; D013997:Time Factors; D046509:Tissue Culture Techniques
D003470:Culture Media; D009930:Organic Chemicals; D019089:Stem Cell Factor; C420642:alpha minimal essential medium
Activation; Kit ligand; Oocyte; Ovary; Sheep