Microscopic Observation Drug Susceptibility Assay for Rapid Diagnosis of Lymph Node Tuberculosis and Detection of Drug Resistance.
In this study, 132 patients with lymphadenopathy were investigated. Fifty-two (39.4%) were diagnosed with tuberculosis (TB). The microscopic observation drug susceptibility (MODS) assay provided rapid (13 days), accurate diagnosis (sensitivity, 65.4%) and reliable drug susceptibility testing (DST). Despite its lower sensitivity than that of other methods, its faster results and simultaneous DST are advantageous in resource-poor settings, supporting the incorporation of MODS into diagnostic algorithms for extrapulmonary TB.
Department of Medical Microbiology, St. George's Hospital, London, United Kingdom Infectious Diseases and Immunity, Imperial College London, London, United Kingdom dannikirwan@yahoo.com.
Daniela E
DE
Kirwan
Infectious Diseases and Immunity, Imperial College London, London, United Kingdom Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
Cesar
C
Ugarte-Gil
Department of International Health, Johns Hopkins University, Baltimore, Maryland, USA Laboratorio de Investigación en Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Lima, Peru.
Robert H
RH
Gilman
Laboratorio de Investigación en Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Lima, Peru.
Luz
L
Caviedes
Department of Cellular Pathology, Barts Health NHS Trust, London, United Kingdom.
Hasan
H
Rizvi
Infectious Diseases and Tropical Medicine Unit, Hospital Nacional Dos de Mayo, Lima, Peru Universidad Nacional Mayor de San Marcos, Lima, Peru Universidad de San Martin de Porres, Lima, Peru.
Eduardo
E
Ticona
Infectious Diseases and Tropical Medicine Unit, Hospital Nacional Dos de Mayo, Lima, Peru.
Gonzalo
G
Chavez
Department of Pulmonology, Hospital Daniel Alcides Carrión, Callao, Peru.
José Luis
JL
Cabrera
Universidad Nacional Mayor de San Marcos, Lima, Peru Infectious Diseases Unit, Hospital Nacional Arzobispo Loayza, Lima, Peru.
Eduardo D
ED
Matos
Infectious Diseases and Immunity, and Wellcome Trust Centre for Global Health Research, Imperial College London, London, United Kingdom IFHAD: Innovation for Health and Development, Universidad Peruana Cayetano Heredia, Lima, Peru.
Carlton A
CA
Evans
Laboratorio de Investigación en Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, Lima, Peru TB Centre, London School of Hygiene & Tropical Medicine, London, United Kingdom.
David A J
DA
Moore
Infectious Diseases and Immunity, Imperial College London, London, United Kingdom.
Jon S
JS
Friedland
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008853:Microscopy / Q000379:methods*; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013997:Time Factors; D014388:Tuberculosis, Lymph Node / Q000175:diagnosis*; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis*; D055815:Young Adult