Transcriptional regulation of dimethyl sulfoxide respiration in a haloarchaeon, Haloferax volcanii.
The halophilic euryarchaeon Haloferax volcanii can grow anaerobically by DMSO respiration. DMSO reductase was induced by DMSO respiration not only under anaerobic growth conditions but also in denitrifying cells of H. volcanii. Deletion of the dmsR gene, encoding a putative regulator for the DMSO reductase, resulted in the loss of anaerobic growth by DMSO respiration. Reporter experiments revealed that only the anaerobic condition was essential for transcription of the dmsEABCD genes encoding DMSO reductase and that transcription was enhanced threefold by supplementation of DMSO. In the ∆dmsR mutant, transcription of the dmsEABCD genes induced by the anaerobic condition was not enhanced by DMSO, suggesting that DmsR is a DMSO-responsive regulator. Transcriptions of the dmsR and mgd genes for Mo-bisMGD biosynthesis were regulated in the same manner as the dmsEABCD genes. These results suggest that the genetic regulation of DMSO respiration in H. volcanii is controlled by at least two systems: one is the DMSO-responsive DmsR, and the other is an unknown anaerobic regulator.
Department of Environment and Energy Systems, Graduate School of Science and Technology, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka, 422-8529, Japan.
Qiuzi
Q
Qi
Department of Biological Science, Faculty of Science, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka, 422-8529, Japan.
Yoshiyasu
Y
Ito
Research Institute of Green Science and Technology, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka, 422-8529, Japan.
Katsuhiko
K
Yoshimatsu
Department of Environment and Energy Systems, Graduate School of Science and Technology, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka, 422-8529, Japan. sbtfuji@ipc.shizuoka.ac.jp.|Department of Biological Science, Faculty of Science, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka, 422-8529, Japan. sbtfuji@ipc.shizuoka.ac.jp.
Taketomo
T
Fujiwara
D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism; D019069:Cell Respiration; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019848:Gene Expression Regulation, Archaeal; D019622:Haloferax volcanii / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D019843:Archaeal Proteins; D007506:Iron-Sulfur Proteins; D010088:Oxidoreductases; C045664:dimethyl sulfoxide reductase; D004121:Dimethyl Sulfoxide
Anaerobic respiration; DMSO reductase; DmsR; Haloferax volcanii; Transcription regulator