DNA-based identification of Peucedanum ostruthium specimens and detection of common adulterants by high-resolution melting curve analysis.
Masterwort (Peucedanum ostruthium, syn. Imperatoria ostruthium, Apiaceae) is an old economic plant in Alpine countries cultivated as ornamental plant and used for spirits and in folk medicine. P. ostruthium is a species that has often been confused with related Apiaceae species or morphologically similar roots or tubers resulting in products of minor quality. Masterwort can be distinguished from other Apiaceae species by nrDNA (ITS1 and ITS2). The analysed chloroplast markers (trnK 5' intron, trnT-trnL, and psbA-trnH), however, showed no species-specific mutations. With the application of two primer pairs amplifying parts of ITS and developed for high-resolution melting curve analysis (HRM) the target species was distinguishable from the other Peucedanum and Apiaceae species of our reference set. A multiplex PCR/HRM was developed to detect adulterations with Gentiana spp., Aconitum napellus and Veratrum album.
Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: Corinna.Schmiderer@vetmeduni.ac.at.
Corinna
C
Schmiderer
Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: Joana.Ruzicka@vetmeduni.ac.at.
Joana
J
Ruzicka
Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: Johannes.Novak@vetmeduni.ac.at.
Johannes
J
Novak
D019661:Apiaceae / Q000145:classification* / Q000235:genetics; D018742:DNA, Chloroplast / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D018517:Plant Roots / Q000235:genetics; D013045:Species Specificity
D018742:DNA, Chloroplast; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer
Apiaceae; DNA-based authentication; HRM; Identification; Imperatoria ostruthium; Peucedanum ostruthium