Realtime (31)P NMR Investigation on the Catalytic Behavior of the Enzyme Adenylate kinase in the Matrix of a Switchable Ionic Liquid.
The integration of highly efficient enzymatic catalysis with the solvation properties of ionic liquids for an environmentally friendly and efficient use of raw materials such as wood requires fundamental knowledge about the influence of relevant ionic liquids on enzymes. Switchable ionic liquids (SIL) are promising candidates for implementation of enzymatic treatments of raw materials. One industrially interesting SIL is constituted by monoethanol amine (MEA) and 1,8-diazabicyclo-[5.4.0]-undec-7-ene (DBU) formed with sulfur dioxide (SO2) as the coupling media (DBU-SO2-MEASIL). It has the ability to solubilize the matrix of lignocellulosic biomass while leaving the cellulose backbone intact. Using a novel (31)P NMR-based real-time assay we show that this SIL is compatible with enzymatic catalysis because a model enzyme, adenylate kinase, retains its activity in up to at least 25 wt % of DBU-SO2-MEASIL. Thus this SIL appears suitable for, for example, enzymatic degradation of hemicellulose.
Department of Chemistry, Umeå University, Linnaeus väg 10, 901 87, Umeå, Sweden.
Per
P
Rogne
Department of Chemistry, Umeå University, Linnaeus väg 10, 901 87, Umeå, Sweden.
Tobias
T
Sparrman
Department of Chemistry, Umeå University, Linnaeus väg 10, 901 87, Umeå, Sweden.
Ikenna
I
Anugwom
Department of Chemistry, Umeå University, Linnaeus väg 10, 901 87, Umeå, Sweden.|Laboratory of Industrial Chemistry and Reaction Engineering, Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Åbo-Turku, 20500, Finland.
Jyri-Pekka
JP
Mikkola
Department of Chemistry, Umeå University, Linnaeus väg 10, 901 87, Umeå, Sweden. magnus.wolf-watz@umu.se.
Magnus
M
Wolf-Watz
D000263:Adenylate Kinase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D018533:Biomass; D052578:Ionic Liquids / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D012995:Solubility
D052578:Ionic Liquids; C036909:lignocellulose; D008031:Lignin; D000263:Adenylate Kinase
NMR spectroscopy; cellulose; enzymes; green chemistry; ionic liquids