A new method to effectively and rapidly generate neurons from SH-SY5Y cells.
It is well known that neurons differentiated from SH-SY5Y cells can serve as cell models for neuroscience research; i.e., neurotoxicity and tolerance to morphine in vitro. To differentiate SH-SY5Y cells into neurons, RA (retinoic acid) is commonly used to produce the inductive effect. However, the percentage of neuronal cells produced from SH-SY5Y cells is low, either from the use of RA treatment alone or from the combined application of RA and other chemicals. In the current study, we used CM-hNSCs (conditioned medium of human neural stem cells) as the combinational inducer with RA to prompt neuronal differentiation of SH-SY5Y cells. We found that neuronal differentiation was improved and that neurons were greatly increased in the differentiated SH-SY5Y cells using a combined treatment of CM-hNSCs and RA compared to RA treatment alone. The neuronal percentage was higher than 80% (about 88%) on the 3rd day and about 91% on the 7th day examined after a combined treatment with CM-hNSCs and RA. Cell maturation and neurite growth of these neuronal cells were also improved. In addition, the use of CM-hNSCs inhibited the apoptosis of RA-treated SH-SY5Y cells in culture. We are the first to report the use of CM-hNSCs in combination with RA to induce neuronal differentiation of RA-treated SH-SY5Y cells. Our method can rapidly and effectively promote the neuronal production of SH-SY5Y cells in culture conditions.
Department of Critical Care Medicine, Qilu Hospital of Shandong University, Shandong University, Jinan, Shandong 250012, China; Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
HongNa
H
Yang
Department of Critical Care Medicine, Qilu Hospital of Shandong University, Shandong University, Jinan, Shandong 250012, China.
Jing
J
Wang
Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
JinHua
J
Sun
R & D, Cell and Tissue Bank of Shandong Province, Jinan, Shandong 250000, China.
XiaoDun
X
Liu
Department of Anatomy, Capital Medical University, Beijing 100069, China.
Wei-Ming
WM
Duan
Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA. Electronic address: tqu@uic.edu.
TingYu
T
Qu
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D017077:Culture Media, Conditioned; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology
D017077:Culture Media, Conditioned; D014212:Tretinoin
CM-hNSCs; Neurons; RA; SH-SY5Y cells; β-tubulin III