Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models.
Traditional cancer treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to tumor hypoxia. While bacterial cancer therapy has the potential to overcome this problem, it comes with the risk of toxicity and infection. To circumvent these issues, this paper investigates the anti-tumor effects of non-viable bacterial derivatives of Clostridium sporogenes. These non-viable derivatives are heat-inactivated C. sporogenes bacteria (IB) and the secreted bacterial proteins in culture media, known as conditioned media (CM). In this project, the effects of IB and CM on CT26 and HCT116 colorectal cancer cells were examined on a 2-Dimensional (2D) and 3-Dimensional (3D) platform. IB significantly inhibited cell proliferation of CT26 to 6.3% of the control in 72 hours for the 2D monolayer culture. In the 3D spheroid culture, cell proliferation of HCT116 spheroids notably dropped to 26.2%. Similarly the CM also remarkably reduced the cell-proliferation of the CT26 cells to 2.4% and 20% in the 2D and 3D models, respectively. Interestingly the effect of boiled conditioned media (BCM) on the cells in the 3D model was less inhibitory than that of CM. Thus, the inhibitive effect of inactivated C. sporogenes and its conditioned media on colorectal cancer cells is established.
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Madhura Satish
MS
Bhave
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Ammar Mansoor
AM
Hassanbhai
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Padmaja
P
Anand
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Kathy Qian
KQ
Luo
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Swee Hin
SH
Teoh
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology; D001691:Biological Therapy / Q000379:methods*; D018929:Cell Culture Techniques / Q000379:methods; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology; D003013:Clostridium / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000382:microbiology* / Q000628:therapy*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D045325:HCT116 Cells; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D018874:Spheroids, Cellular / Q000187:drug effects / Q000382:microbiology / Q000502:physiology
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned