PFTK1 Promotes Gastric Cancer Progression by Regulating Proliferation, Migration and Invasion.
PFTK1, also known as PFTAIRE1, CDK14, is a novel member of Cdc2-related serine/threonine protein kinases. Recent studies show that PFTK1 is highly expressed in several malignant tumors such as hepatocellular carcinoma, esophageal cancer, breast cancer, and involved in regulation of cell cycle, tumors proliferation, migration, and invasion that further influence the prognosis of tumors. However, the expression and physiological significance of PFTK1 in gastric cancer remain unclear. In this study, we analyzed the expression and clinical significance of PFTK1 by Western blot in 8 paired fresh gastric cancer tissues, nontumorous gastric mucosal tissues and immunohistochemistry on 161 paraffinembedded slices. High PFTK1 expression was correlated with the tumor grade, lymph node invasion as well as Ki-67. Through Cell Counting Kit (CCK)-8 assay, flow cytometry, colony formation, wound healing and transwell assays, the vitro studies demonstrated that PFTK1 overexpression promoted proliferation, migration and invasion of gastric cancer cells, while PFTK1 knockdown led to the opposite results. Our findings for the first time supported that PFTK1 might play an important role in the regulation of gastric cancer proliferation, migration and would provide a novel promising therapeutic strategy against human gastric cancer.
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu, China.
Lei
L
Yang
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu, China.
Jia
J
Zhu
Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
Hua
H
Huang
Department of Pathology, Nantong first people's hospital, Nantong, Jiangsu, China.
Qichang
Q
Yang
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu, China.
Jing
J
Cai
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu, China.
Qiuhong
Q
Wang
Department of Pathogen Biology, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong 226001, Jiangsu, China.
Junya
J
Zhu
Department of Pathogen Biology, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong 226001, Jiangsu, China.
Mengting
M
Shao
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu, China.
Jinzhang
J
Xiao
Department of Pathology, Nantong first people's hospital, Nantong, Jiangsu, China.
Jie
J
Cao
Department of Pathogen Biology, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong 226001, Jiangsu, China.
Xiaodan
X
Gu
Department of Pathogen Biology, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong 226001, Jiangsu, China.
Shusen
S
Zhang
Department of Pathogen Biology, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong 226001, Jiangsu, China.
Yingying
Y
Wang
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering; D013270:Stomach / Q000473:pathology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000401:mortality / Q000473:pathology*
D034741:RNA, Small Interfering; C515076:CDK14 protein, human; D018844:Cyclin-Dependent Kinases