Development of prototypes of bioactive packaging materials based on immobilized bacteriophages for control of growth of bacterial pathogens in foods.
Due to lack of adequate control methods to prevent contamination in fresh produce and growing consumer demand for natural products, the use of bacteriophages has emerged as a promising approach to enhance safety of these foods. This study sought to control Listeria monocytogenes in cantaloupes and RTE meat and Escherichia coli O104:H4 in alfalfa seeds and sprouts under different storage conditions by using specific lytic bacteriophage cocktails applied either free or immobilized. Bacteriophage cocktails were introduced into prototypes of packaging materials using different techniques: i) immobilizing on positively charged modified cellulose membranes, ii) impregnating paper with bacteriophage suspension, and iii) encapsulating in alginate beads followed by application of beads onto the paper. Phage-treated and non-treated samples were stored for various times and at temperatures of 4°C, 12°C or 25°C. In cantaloupe, when free phage cocktail was added, L. monocytogenes counts dropped below the detection limit of the plating technique (<1 log CFU/g) after 5 days of storage at both 4°C and 12°C. However, at 25°C, counts below the detection limit were observed after 3 and 6h and a 2-log CFU/g reduction in cell numbers was seen after 24h. For the immobilized Listeria phage cocktail, around 1-log CFU/g reduction in the Listeria count was observed by the end of the storage period for all tested storage temperatures. For the alfalfa seeds and sprouts, regardless of the type of phage application technique (spraying of free phage suspension, bringing in contact with bacteriophage-based materials (paper coated with encapsulated bacteriophage or impregnated with bacteriophage suspension)), the count of E. coli O104:H4 was below the detection limit (<1 log CFU/g) after 1h in seeds and about a 1-log cycle reduction in E. coli count was observed on the germinated sprouts by day 5. In ready-to-eat (RTE) meat, LISTEX™ P100, a commercial phage product, was able to significantly reduce the growth of L. monocytogenes at both storage temperatures, 4°C and 10°C, for 25 days regardless of bacteriophage application format (immobilized or non-immobilized (free)). In conclusion, the developed phage-based materials demonstrated significant antimicrobial effect, when applied to the artificially contaminated foods, and can be used as prototypes for developing bioactive antimicrobial packaging materials capable of enhancing the safety of fresh produce and RTE meat.
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Ayesha
A
Lone
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada; Department of Microbiology, Faculty of Science, Ain Shams University, Egypt. Electronic address: hanany@alumni.uoguelph.ca.
Hany
H
Anany
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Mohammed
M
Hakeem
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Louise
L
Aguis
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Anne-Claire
AC
Avdjian
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Marina
M
Bouget
Département de chimie, Université de Montréal, CP6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
Arash
A
Atashi
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Luba
L
Brovko
Département de chimie, Université de Montréal, CP6128 Succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada.
Dominic
D
Rochefort
Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
Mansel W
MW
Griffiths
D000464:Alginates; D061046:Biological Control Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D028463:Cucumis melo / Q000382:microbiology; D004926:Escherichia coli / Q000254:growth & development* / Q000821:virology; D005506:Food Contamination / Q000517:prevention & control*; D018857:Food Packaging / Q000379:methods*; D061353:Food Storage / Q000379:methods*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000821:virology; D008460:Meat / Q000382:microbiology; D000455:Medicago sativa / Q000382:microbiology; D017900:Myoviridae / Q000378:metabolism*; D013696:Temperature
D000464:Alginates; D061046:Biological Control Agents; D006603:Hexuronic Acids; D020723:Glucuronic Acid
Bacteriophages; Biocontrol; E. coli O104:H4; Immobilization; Listeria monocytogenes; RTE food