Development of ELISA and Colloidal Gold-PAb Conjugate-Based Immunochromatographic Assay for Detection of Abrin-a.
When abrin-a was combined with several polyclonal antibodies (PAb), the detection limit could be increased. In this way, a monoclonal antibody (capture) and polyclonal antibody (detection) sandwich enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-PAb conjugate-based immunochromatographic assay for detection of abrin-a were developed. The ELISA had a detection limit of 3.9 ng/mL for abrin-a in standard solution and 7.8 ng/mL in soybean milk, and was more sensitive than polyclonal antibody (capture) and monoclonal antibody (detection) ELISA, which had a detection limit of 15.6 ng/mL. The test strip had a detection range of 50 to 500 ng/mL for abrin-a and a detection limit in standard solution or soybean milk samples of 50 ng/mL. However, the test strip had a reduced detection capability compared with a colloidal gold-monoclonal antibody conjugate-based immunochromatographic assay test strip, which had a lower detection limit of 10 ng/mL. The developed ELISAs and test strip show the specificity towards abrin-a and have no cross-reactivity towards abrin-b, -c, -d, ricin, or the agglutinins from either castor beans or rosary peas.
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Chuang
C
Xu
2 College of Animal Science and Veterinary Medicine, Jilin University , Changchun, China .
Xiaobing
X
Li
2 College of Animal Science and Veterinary Medicine, Jilin University , Changchun, China .
Guowen
G
Liu
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Chuchu
C
Xu
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Cheng
C
Xia
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Ling
L
Wu
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Hongyou
H
Zhang
1 College of Animal Science And Veterinary Medicine, Heilongjiang Bayi Agricultural University , China .
Wei
W
Yang
D000036:Abrin / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D017979:Gold Colloid / Q000276:immunology*; D057230:Limit of Detection; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000276:immunology
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D000036:Abrin