EDIN-B Promotes the Translocation of Staphylococcus aureus to the Bloodstream in the Course of Pneumonia.
It is crucial to define risk factors that contribute to host invasion by Staphylococcus aureus. Here, we demonstrate that the chromosomally encoded EDIN-B isoform from S. aureus contributes to the onset of bacteremia during the course of pneumonia. Deletion of edinB in a European lineage community-acquired methicillin resistant S. aureus (CA-MRSA) strain (ST80-MRSA-IV) dramatically decreased the frequency and magnitude of bacteremia in mice suffering from pneumonia. This deletion had no effect on the bacterial burden in both blood circulation and lung tissues. Re-expression of wild-type EDIN-B, unlike the catalytically inactive mutant EDIN-R185E, restored the invasive characteristics of ST80-MRSA-IV.
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. johan_courjon@hotmail.fr.
Johan
J
Courjon
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. munro@unice.fr.
Patrick
P
Munro
CIRI, International Center for Infectiology Research, Inserm U1111, Université Lyon 1, Ecole Normale Supérieure de Lyon, CNRS UMR5308, 7 Rue Guillaume Paradin, Lyon 69372, France. yvonne.benito@chu-lyon.fr.
Yvonne
Y
Benito
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. Orane.Visvikis@unice.fr.
Orane
O
Visvikis
CIRI, International Center for Infectiology Research, Inserm U1111, Université Lyon 1, Ecole Normale Supérieure de Lyon, CNRS UMR5308, 7 Rue Guillaume Paradin, Lyon 69372, France. coralie.bouchiat@chu-lyon.fr.
Coralie
C
Bouchiat
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. boyerl@unice.fr.
Laurent
L
Boyer
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. doye@unice.fr.
Anne
A
Doye
CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille 13385, France. Hubert.Lepidi@ap-hm.fr.
Hubert
H
Lepidi
CNRS UMR 7278, IRD198, INSERM U1095, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille 13385, France. eric.ghigo@univ-amu.fr.
Eric
E
Ghigo
Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes 30029, France. jphlavigne@hotmail.com.
Jean-Philippe
JP
Lavigne
CIRI, International Center for Infectiology Research, Inserm U1111, Université Lyon 1, Ecole Normale Supérieure de Lyon, CNRS UMR5308, 7 Rue Guillaume Paradin, Lyon 69372, France. francois.vandenesch@univ-lyon1.fr.
François
F
Vandenesch
INSERM U1065, Equipe Labellisée Ligue Contre le Cancer, Centre Méditerranéen de Médecine Moléculaire (C3M), Université de Nice Sophia-Antipolis, Nice 06204, France. Lemichez@unice.fr.
Emmanuel
E
Lemichez
D000818:Animals; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics*; D018988:Bacterial Translocation / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D014774:Virulence
D001426:Bacterial Proteins; C067120:epidermal cell differentiation inhibitor, Staphylococcus aureus
bacteremia; methicillin-resistant Staphylococcus aureus; toxins; virulence factor