Chemokine receptor expression on monocytes from healthy individuals.
Chronic immune mediated inflammation is characterized by continuous chemokine mediated recruitment and activation of pro-inflammatory cells, monocytes in particular. We believe that an evaluation of the recruitment profile of monocytes during healthy condition is essential for the understanding of cellular response in disease. For this, we have established normal reference values and 95% confidence intervals for receptor expression of 20 chemokine receptors on monocyte subsets; classical (CD14+ CD16−), non-classical (CD14+ CD16+) and HLA-DRhi monocytes from 20 healthy controls using flow cytometry. We demonstrate significant differences in the chemokine receptor expression profiles and high correlation between fraction of cells and level of expression. This is the first global approach to provide a platform for comparable evaluation of cell recruitment during normal and under inflammatory conditions. This will be useful when exploring chemokine–chemokine receptor interactions, inhibition of chemokine signaling and selective removal of migrating cells, which are new treatment strategies in immune mediated diseases.
Department of Medicine, Translational Immunology Unit, Karolinska Institutet, Stockholm, Sweden.
Katarina Glise
KG
Sandblad
Department of Medicine, Translational Immunology Unit, Karolinska Institutet, Stockholm, Sweden; ITH, Karolinska University Hospital, Stockholm, Sweden.
Petra
P
Jones
ITH, Karolinska University Hospital, Stockholm, Sweden.
Martina Jones
MJ
Kostalla
Department of Medicine, Translational Immunology Unit, Karolinska Institutet, Stockholm, Sweden.
Ludvig
L
Linton
Department of Medicine, Translational Immunology Unit, Karolinska Institutet, Stockholm, Sweden; ITH, Karolinska University Hospital, Stockholm, Sweden.
Hans
H
Glise
Department of Medicine, Translational Immunology Unit, Karolinska Institutet, Stockholm, Sweden. Electronic address: ola.winqvist@karolinska.se.
Ola
O
Winqvist
D000328:Adult; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D054388:Receptors, CCR / Q000378:metabolism; D054387:Receptors, CXCR / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism*
D054388:Receptors, CCR; D054387:Receptors, CXCR; D019707:Receptors, Chemokine
Chemokine receptor; Immune mediated disease; Monocytes; Reference values