Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India.
A taxonomic description of bacteria was deduced from 5.78 Mb metagenomic sequence retrieved from Tulsi Shyam hot spring, India using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome contained 10,893 16S rDNA sequences that were analyzed by MG-RAST server to generate the comprehensive profile of bacteria. Metagenomic data are available at EBI under EBI Metagenomics database with accession no. ERP009559. Metagenome sequences represented the 98.2% bacteria origin, 1.5% of eukaryotic and 0.3% were unidentified. A total of 16 bacterial phyla demonstrating 97 families and 287 species were revealed in the hot spring metagenome. Most abundant phyla were Firmicutes (65.38%), Proteobacteria (21.21%) and unclassified bacteria (10.69%). Whereas, Peptostreptococcaceae (37.33%), Clostridiaceae (23.36%), and Enterobacteriaceae (16.37%) were highest reported families in metagenome. Ubiquitous species were Clostridium bifermentans (17.47%), Clostridium lituseburense (13.93%) and uncultured bacterium (10.15%). Our data provide new information on hot spring bacteria and shed light on their abundance, diversity, distribution and coexisting organisms.
Department of Microbiology, Shree Ramkrishna Institute of Computer Education and Applied Sciences, Surat, India ; Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, India.
Anjana
A
Ghelani
Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, India.
Rajesh
R
Patel
Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, India.
Amitsinh
A
Mangrola
Department of Biotechnology, Veer Narmad South Gujarat University, Surat, India.
Pravin
P
Dudhagara
Bacterial diversity; Cultivation-independent; Hot spring; Metagenome; Tulsi Shyam; bTEFAP