Selecting molecular therapeutic drug targets based on the expression profiles of intrahepatic cholangiocarcinomas and miRNA-mRNA regulatory networks.
The incidence of intrahepatic cholangiocarcinoma (ICC) is increasing yearly, making it the second most common carcinoma after hepatocellular carcinoma among primary malignant liver tumors. Integrated miRNA and mRNA analysis is becoming more frequently used in antitumor ICC treatment. However, this approach generates vast amounts of data, which leads to difficulties performing comprehensive analyses to identify specific therapeutic drug targets. In this study, we provide an in-depth analysis of ICC function, identifying potential highly potent antitumor drugs for antitumor therapy. Two sets of whole genome expression profiles were obtained from the Gene Expression Omnibus (GEO) database. Using modular bioinformatic analysis, six core functional modules were identified for ICC. Based on a Fisher's test of the Cmap small molecule drug database, 65 drug components were identified that regulated the genes of these six core modules. Literature mining was then used to identify 15 new potential antitumor drugs.
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Boshi
B
Sun
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Changming
C
Xie
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Tongsen
T
Zheng
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Dalong
D
Yin
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Jiabei
J
Wang
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Yingjian
Y
Liang
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Yuejin
Y
Li
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Guangchao
G
Yang
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Huawen
H
Shi
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Tiemin
T
Pei
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Jihua
J
Han
Key Laboratory of Hepatosplenic Surgery, Chinese Ministry of Education, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.
Lianxin
L
Liu
D001650:Bile Duct Neoplasms / Q000188:drug therapy / Q000235:genetics*; D018281:Cholangiocarcinoma / Q000188:drug therapy / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D053263:Gene Regulatory Networks / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D054852:Small Molecule Libraries / Q000494:pharmacology
D035683:MicroRNAs; D012333:RNA, Messenger; D054852:Small Molecule Libraries