Mass-scale donor red cell genotyping using real-time array technology.
Blood centers are in the unique position to evaluate large numbers of blood donations for antigen-negative blood types. The limitations with the use of hemagglutination, however, can be circumvented with red cell genotyping. The reagents used for genotyping are synthesized and can be designed for any of the known blood group antigen single nucleotide polymorphisms that are associated with blood group antigen expression. There is interest in the application of mass-scale red cell genotyping of blood donors to find rare phenotypes and rare combinations of antigens. When performed on donors who are predicted to donate again after testing, integrating the genotype information with existing donor data and demographics provides the blood center with real-time information to identify the common clinically relevant blood group antigens demanded by hospital transfusion services. This review outlines a red cell genotype methodology using TaqMan chemistry and existing algorithms and data handling to gain the full value of mass-scale red cell genotyping of blood donors.
PhD, FCSMLS(D) (corresponding author), Director, Immunohematology and Transfusion Services, Diagnostic Laboratories, Blood Center of Wisconsin, and Senior Investigator, Blood Research Institute.
Gregory A
GA
Denomme
Manager, Immunohematology and Transfusion Services, Blood Center of Wisconsin, 638 North 18th Street, P.O. Box 2178, Milwaukee, WI 53201-2178.
Michael J
MJ
Schanen
D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D060005:Genotyping Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
D001789:Blood Group Antigens