Antimicrobial Activity Evaluation on Silver Doped Hydroxyapatite/Polydimethylsiloxane Composite Layer.
The goal of this study was the preparation, physicochemical characterization, and microbiological evaluation of novel hydroxyapatite doped with silver/polydimethylsiloxane (Ag:HAp-PDMS) composite layers. In the first stage, the deposition of polydimethylsiloxane (PDMS) polymer layer on commercially pure Si disks has been produced in atmospheric pressure corona discharges. Finally, the new silver doped hydroxyapatite/polydimethylsiloxane composite layer has been obtained by the thermal evaporation technique. The Ag:HAp-PDMS composite layers were characterized by various techniques, such as Scanning Electron Microscopy (SEM), Glow Discharge Optical Emission Spectroscopy (GDOES), and X-ray photoelectron spectroscopy (XPS). The antimicrobial activity of the Ag:HAp-PDMS composite layer was assessed against Candida albicans ATCC 10231 (ATCC-American Type Culture Collection) by culture based and confirmed by SEM and Confocal Laser Scanning Microscopy (CLSM) methods. This is the first study reporting the antimicrobial effect of the Ag:HAp-PDMS composite layer, which proved to be active against Candida albicans biofilm embedded cells.
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania.
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Ciobanu
National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania.
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Groza
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania.
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Iconaru
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania ; Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG1, 077125 Magurele, Romania.
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Popa
Horiba Jobin Yvon SAS, 16-18 Rue du Canal, 91165 Longjumeau Cedex, France.
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Chapon
Microbiology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania ; Earth, Environmental and Life Sciences Section, Research Institute of the University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania.
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Chifiriuc
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.
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Hristu
Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.
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Stanciu
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania.
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Negrila
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania.
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Ghita
National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania.
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Ganciu
National Institute for Materials Physics, P.O. Box MG 07, 077125 Magurele, Romania.
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D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
D000890:Anti-Infective Agents; D004129:Dimethylpolysiloxanes; D012834:Silver; C013830:baysilon; D017886:Durapatite