Expression and Role of CD166 in the Chronic Kidney Disease.
BACKGROUND
CD166, an adhesion molecule of the immunoglobulin superfamily, is one of the crucial effectors that traffic lymphocytes into tissues. Till now, the expression and role of CD166 in the chronic kidney disease remains unknown.


OBJECTIVE
In the present study, we are to examine the expression of CD166 in the chronic kidney disease, and to explore its function with CD4+ T cells.


METHODS
CD166 expression was tested by Flow Cytometry (FACS) in the primary macrophages stimulated with LPS. In vivo, the expression of CD166 and CD4 were examined in the kidney tissues of adriamycin-induced nephropathy (AN) mice by immnohistochemistry. Macrophages and lymphocytes were co-cultured, the interaction between CD166 and CD4 was tested by immunofluorescent staining. Furthermore, the effects of CD166 on the activation and proliferation of T cells were explored.


RESULTS
In this study, CD166 expression was found to be upregulated on activated macrophages and glomerular endothelia in the adriamycin-induced nephropathy (AN) mice and CD4+ T cells were increased with CD166 expression in the AN mice. The interaction between macrophages and CD4+ T cells indicated that CD166 played a key role in the recruitment of lymphocytes in the chronic kidney disease, and neither proliferation nor activation of T cells was affected by CD166.


CONCLUSIONS
CD166 expressed on macrophages and endothelia in AN kidney, and the function was related to the recruitment of CD4+ T cells into inflamed kidney, indicating that CD166 may be a potential target for reducing the inflammatory infiltrates in the chronic kidney disease.
Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Yan
Y
Sun
Centre for Transplantation and Renal Research, University of Sydney at Westmead Millennium Institute, Sydney, Australia.
Yiping
Y
Wang
Centre for Transplantation and Renal Research, University of Sydney at Westmead Millennium Institute, Sydney, Australia.
Qi
Q
Cao
The High Tech Centre, Westmead Millennium Institute, University of Sydney Westmead, Sydney, Australia.
Hong
H
Yu
Centre for Transplantation and Renal Research, University of Sydney at Westmead Millennium Institute, Sydney, Australia.
Dong
D
Zheng
Centre for Transplantation and Renal Research, University of Sydney at Westmead Millennium Institute, Sydney, Australia.
Ya
Y
Wang
Centre for Transplantation and Renal Research, University of Sydney at Westmead Millennium Institute, Sydney, Australia.
David C H
DC
Harris
Activated-Leukocyte Cell Adhesion Molecule; Chronic Kidney Disease; Lymphocytes