Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties.
In this study, we compared the amino acid, organic acid and sugar profiles of 3 different varieties of dry beans (black bean [BB], dark red bean [DRB], and cranberry bean [CB]). The efficiency of the 2 commonly used extraction solvents (water and methanol:chloroform:water [2.5:1:1, v/v/v/]) for cultivar differentiation based on their metabolic profile was also investigated. The results showed that the BB contained the highest concentration of amino acids followed by DRB and CB samples. Phenylalanine, a precursor for the biosynthesis of phenolic secondary metabolites was detected at low concentration in CB samples and correlated with the reduced anthocyanins content in CB extract as documented in the published literature. Comparing the extractability of 2 extraction solvents, methanol:chloroform:water (2.5:1:1, v/v/v/) showed higher recoveries of amino acids from 3 beans, whereas, sugars were extracted in higher concentration with water. Analytically, gas chromatography detected sugars (9), amino acids (11), and organic acids (3) in a single run after derivatization of the extracts. In comparison, ion chromatography detected only sugars in a single run without any derivatization step with the tested procedure. Bean samples are better differentiated by the sugar content extracted with water as compared to the aqueous organic solvent extracts using partial least-square discriminant analysis.
Food Composition Methods Development Lab, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Beltsville, Md., 20705, U.S.A.
K M Maria
KM
John
Food Composition Methods Development Lab, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Dept. of Agriculture, Beltsville, Md., 20705, U.S.A.
Devanand
D
Luthria
D000143:Acids / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D006801:Humans; D027805:Phaseolus / Q000737:chemistry* / Q000145:classification; D010636:Phenols / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013045:Species Specificity
D000143:Acids; D000596:Amino Acids; D002241:Carbohydrates; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents
amino acid; dry beans; gas and ion chromatography mass spectrometry; metabolic profiles; sugars