The protective role of montelukast against intestinal ischemia-reperfusion injury in rats.
Several drugs are effective in attenuating intestinal ischemia-reperfusion injury (IRI); however little is known about the effect of montelukast. Fifty rats were randomly assigned to 3 groups: model group (operation with clamping), sham group (operation without clamping), and study group (operation with clamping and 0.2, 2 and 20 mg/kg montelukast pretreatment). Intestinal ischemia-reperfusion was performed by occlusion (clamping) of the arteria mesenterica anterior for 45 min, followed by 24 h reperfusion. Intestinal IRI in the model group led to severe damage of the intestinal mucosa, liver and kidney. The Chiu scores of the intestines from the study group (2 and 20 mg/kg) were lower than that of the model group. Intestinal IRI induced a marked increase in CysLTR1, Caspase-8 and -9 expression in intestine, liver and kidney, which were markedly reduced by preconditioning with 2 mg/kg montelukast. Preconditioning with 2 g/kg montelukast significantly attenuated hepatic tissue injury and kidney damage, and decreased plasma interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels in plasma after intestinal IRI. In conclusion, preconditioning with montelukast could attenuate intestinal IRI and the subsequent systemic inflammatory response in rats.
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Shenbao
S
Wu
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Xuxing
X
Zhu
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Zhonghai
Z
Jin
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Xiuping
X
Tong
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Liqin
L
Zhu
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Xiaofei
X
Hong
Department of Gastroenterology, the Affiliated Yiwu Hospital of Wenzhou Medical College, Yiwu 322000, PR China.
Xianfei
X
Zhu
Department of Gastroenterology, the Affiliated Jiangyin Hospital of Nantong University Medical School, Jiangyin 214400, PR China.
Pengfei
P
Liu
Department of Gastroenterology, the Affiliated Jiangyin Hospital of Nantong University Medical School, Jiangyin 214400, PR China.
Weidong
W
Shen
D000085:Acetates / Q000494:pharmacology / Q000627:therapeutic use*; D000209:Acute-Phase Proteins / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D003521:Cyclopropanes; D015850:Interleukin-6 / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D018077:Receptors, Leukotriene / Q000235:genetics / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D013440:Sulfides; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D000085:Acetates; D000209:Acute-Phase Proteins; D003521:Cyclopropanes; D015850:Interleukin-6; C097278:Lcn2 protein, rat; D000071068:Lipocalin-2; D054834:Lipocalins; D020011:Protective Agents; D011518:Proto-Oncogene Proteins; D011804:Quinolines; D012333:RNA, Messenger; D018077:Receptors, Leukotriene; D013440:Sulfides; D014409:Tumor Necrosis Factor-alpha; C412375:cysteinyl leukotriene receptor 2; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D053181:Caspase 8; D053453:Caspase 9; C093875:montelukast