[Immobilization of Estrogen-degrading Bacteria to Remove the 17β-estradiol and Diethylstilbestrol from Polluted Water and Cow Dung].
Estradiol (E2) and diethylstilbestrol (DES) can be enriched in sewage and cow dung, posing serious threats to human and ecological health. Strain Rhodococcus sp. JX-2 and strain Serratia sp. S, which could degrade 17β-estradiol and diethylstilbestrol, respectively, were immobilized by alginate and then added into sewage and cow dung to remove E2 and DES. The immobilization was determined by orthogonal experiment, and the removal of E2 and DES from sewage and cow dung was compared between treatments of immobilized bacteria, free bacteria and control without bacteria. The influencing factors including inoculation amount, pH value, moisture content, turning time on the removal of E2 and DES were investigated. The optimal conditions of JX-2 and S immobilization were as follows: Strain JX-2: strain S (V/V) 1: 1, alginate concentration 5%, calcium chloride concentration 4%, bacteria-cement ratio 1 : 2. The immobilized strains removed 99. 42% and 84. 59% of the 2 mg.L-1 E2 and DES under laboratory conditions, respectively. The optimal conditions for E2 and DES removal from water by the immobilized strains were as follows: 300 g.L-1 inoculation volume of immobilized strains and pH 5. 0-6. 0. Immobilized bacteria could completely remove DES and remove 95. 85% of E2 from water. The optimal conditions for E2 and DES removal from cow dung by the immobilized strains were: inoculation volume 600 g.kg-1, moisture content 70% and pile turning time 12 h. The immobilized bacteria could completely remove E2 and remove 97. 41% of DES from cow dung.
Xin
X
Li
Wan-ting
WT
Ling
Jing-xian
JX
Liu
Min-xia
MX
Sun
Yan-zheng
YZ
Gao
Juan
J
Liu
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002417:Cattle; D018914:Cells, Immobilized; D004054:Diethylstilbestrol / Q000302:isolation & purification*; D004958:Estradiol / Q000302:isolation & purification*; D008372:Manure; D012240:Rhodococcus / Q000378:metabolism; D012705:Serratia / Q000378:metabolism; D012722:Sewage; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D008372:Manure; D012722:Sewage; D014874:Water Pollutants, Chemical; D004958:Estradiol; D004054:Diethylstilbestrol