Diagnostic utility of BNP, corin and furin as biomarkers for cardiovascular complications in type 2 diabetes mellitus patients.
BACKGROUND
The number of patients with type 2 diabetes mellitus (T2DM) is progressively increasing, and diabetic cardiovascular complications have become a public health problem. Brain or B-type natriuretic peptide (BNP) is a cardiac hormone synthesized as a pre-pro-peptide. pro-BNP is produced by cleaving the signal peptide then two proprotein convertases, corin and furin cleave pro-BNP to form a biologically active hormone. Two corin single nucleotide polymorphisms (SNPs) have been reported to alter corin protein conformation and impair its biological activity.


OBJECTIVE
We aimed to investigate the potential role of corin and furin in comparison to BNP as biomarkers for predicting cardiovascular complications in T2DM patients. The association of corin gene SNPs with corin levels was also examined.


METHODS
Seventy-five subjects were recruited in this study, including 25 T2DM patients with complications, 25 T2DM patients without complications as well as 25 healthy subjects. Plasma BNP, corin and furin levels were measured using enzyme-linked immunosorbent assays. Two corin SNPs were genotyped using allele specific oligonucleotide-polymerase chain reaction.


RESULTS
Both furin and BNP were found to be more sensitive than corin (80% versus 56%, p = 0.008), whereas furin showed higher specificity when compared to BNP (96% versus 84%, p = 0.041) and corin (96% versus 64%, p < 0.0001) in predicting cardiovascular complications in T2DM patients. Corin SNPs are not associated with corin levels, neither in the entire study cohort nor in the subgroup of T2DM patients with cardiovascular complications (p > 0.05).


CONCLUSIONS
Furin may be useful, either alone or in combination with other biomarkers, for cardiovascular risk stratification assessment in T2DM patients.
a Department of Biochemistry , Faculty of Science, Ain Shams University , Cairo , Egypt .
Shadia A
SA
Fathy
a Department of Biochemistry , Faculty of Science, Ain Shams University , Cairo , Egypt .
Fatma F
FF
Abdel Hamid
b Biochemistry Department , Faculty of Science, Islamic University , Gaza , Palestine .
Baker M
BM
Zabut
c Cardiology Department , El-Shifa Hospital , Gaza , Palestine , and.
Amal F
AF
Jamee
a Department of Biochemistry , Faculty of Science, Ain Shams University , Cairo , Egypt .
Mohamed A M
MA
Ali
d General Directorate of Human Resources Development, Ministry of Health , Gaza , Palestine.
Ayman M
AM
Abu Mustafa
D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002318:Cardiovascular Diseases / Q000097:blood* / Q000150:complications / Q000175:diagnosis; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications / Q000235:genetics; D005260:Female; D045683:Furin / Q000097:blood*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012372:ROC Curve; D012697:Serine Endopeptidases / Q000097:blood* / Q000235:genetics
D015415:Biomarkers; D020097:Natriuretic Peptide, Brain; C119812:CORIN protein, human; D012697:Serine Endopeptidases; D045683:Furin
BNP; cardiovascular complications; corin; furin; type 2 diabetes mellitus