Intravenous infusion of H2-saline suppresses oxidative stress and elevates antioxidant potential in Thoroughbred horses after racing exercise.
Upon intensive, exhaustive exercise, exercise-induced reactive oxygen species may exceed the antioxidant defence threshold, consequently resulting in muscular damage or late-onset chronic inflammation. Recently, the therapeutic antioxidant and anti-inflammatory effects of molecular hydrogen (H2) for human rheumatoid arthritis have been demonstrated. However, it is also important to clarify the effects of administrating H2 in large animals other than humans, as H2 is thought to reach the target organ by passive diffusion upon delivery from the blood flow, indicating that the distance from the administration point to the target is critical. However, data on the effects of H2 on oxidative stress in real-life exhaustive exercise in large animals are currently lacking. We here investigated 13 Thoroughbred horses administered intravenous 2-L saline with or without 0.6-ppm H2 (placebo, N = 6; H2, N = 7) before participating in a high-intensity simulation race. Intravenous H2-saline significantly suppressed oxidative stress immediately, 3 h, and 24 h after the race, although the antioxidant capability was not affected throughout the study. The serum creatine kinase, lactate, and uric acid levels were increased in both groups. Taken together, these results indicate that intravenous H2-saline can significantly and specifically suppress oxidative stress induced after exhaustive racing in Thoroughbred horses.
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.|Yamazaki Horse Clinic, 2-1-1 Mikoma, Miho-mura, Inasiki-gun, Ibaraki 300-0415, Japan.
Masahiko
M
Yamazaki
Japan Racing Association, Deputy Manager, Equine Department Veterinary Section, 11-1, Roppongi 6-chome Minato-ku, Tokyo 106-8401, Japan.
Kanichi
K
Kusano
Department of Rheumatology and Orthopaedic Surgery, Huis Ten Bosch Satellite H2 Clinic Hakata, 2-1 Gion, Hakata-ku, Fukuoka 812-0038, Japan.|Anicom Speciality Medical Institute, 1-5-22 Shimoochiai, Shinjuku-ku, Tokyo 161-0033, Japan.
Toru
T
Ishibashi
Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.
Masataka
M
Kiuchi
Graduate School Department of Interdisciplinary Research, University of Yamanashi 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.
Katsuhiro
K
Koyama
D000693:Anaerobiosis; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D006736:Horses; D007262:Infusions, Intravenous; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D012965:Sodium Chloride / Q000737:chemistry*
D000975:Antioxidants; D012965:Sodium Chloride