The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity.
This study was designed to evaluate the cytotoxicity of four dentin bonding agents and the effects of an antioxidant addition. Group A: G-aenial Bond, Group B: Optibond All in One, Group C: Gluma Self Etch and Group D: Clearfil S(3) Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.
Ege University School of Dentistry, Department of Restorative Dentistry.
Cigdem
C
Atalayin
Guliz
G
Armagan
Sibel
S
Konyalioglu
Hande
H
Kemaloglu
Huseyin
H
Tezel
Zeynep
Z
Ergucu
Aysegul
A
Keser
Taner
T
Dagci
Banu
B
Onal
D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000633:toxicity*; D004249:DNA Damage; D001840:Dental Bonding; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D005976:Glutaral; D008689:Methacrylates; D051379:Mice; D018384:Oxidative Stress; D011109:Polymethacrylic Acids; D017382:Reactive Oxygen Species; D019279:Resin Cements; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
C505694:Clearfil S3 Bond; D003603:Cytotoxins; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; C092987:Optibond; D011109:Polymethacrylic Acids; D017382:Reactive Oxygen Species; D019279:Resin Cements; D013267:Stilbenes; C044372:Gluma; D000077185:Resveratrol; D005976:Glutaral