Novel water-soluble methanofullerenes C60[C13H18O4(OH)4]6 and C60[C9H10O4(OH)4]6: Promising uncouplers of respiration and phosphorylation.
Here, we report for the first time on two novel water-soluble polyol-methanofullerenes which uncouple respiration and oxidative phosphorylation. A cytofluorimetric JC-1-based ratiometric assay was used to quantify mitochondrial potential Ψm in Yarrowia lipolytica cells exposed to the fullerenes tested. Both methanofullerenes significantly downregulated Ψm, thereby decreasing the subset of cells with high mitochondrial potential compared with intact control cells. The Ψm-low subset of Yarrowia lipolytica cells resulted from methanofullerenes exposure preserved physiological cell size and granularity patterns.
Kazan (Volga Region) State University, Kremlevskaya Str. 18, 420008 Kazan, Russian Federation. Electronic address: nvkalacheva@ya.ru.
Natalia V
NV
Kalacheva
A.E. Arbuzov Institute of Organic and Physical Chemistry of Kazan Scientific Centre of RAS, Arbuzov Str. 8, 420088 Kazan, Russian Federation.
Valentina P
VP
Gubskaya
A.E. Arbuzov Institute of Organic and Physical Chemistry of Kazan Scientific Centre of RAS, Arbuzov Str. 8, 420088 Kazan, Russian Federation.
Guzel M
GM
Fazleeva
Kazan (Volga Region) State University, Kremlevskaya Str. 18, 420008 Kazan, Russian Federation.
Gulzada R
GR
Igtisamova
A.E. Arbuzov Institute of Organic and Physical Chemistry of Kazan Scientific Centre of RAS, Arbuzov Str. 8, 420088 Kazan, Russian Federation.
Ildus A
IA
Nuretdinov
Kazan (Volga Region) State University, Kremlevskaya Str. 18, 420008 Kazan, Russian Federation.
Albert A
AA
Rizvanov
Kazan State Medical Academy, Mushtary Str. 11, 420010 Kazan, Russian Federation; Kazan (Volga Region) State University, Kremlevskaya Str. 18, 420008 Kazan, Russian Federation. Electronic address: rkb2_rt@mail.ru.
Georgi V
GV
Cherepnev
D005434:Flow Cytometry; D037741:Fullerenes / Q000494:pharmacology*; D008564:Membrane Potentials; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation; D012995:Solubility; D014475:Uncoupling Agents / Q000494:pharmacology*; D014867:Water; D025062:Yarrowia / Q000187:drug effects* / Q000378:metabolism
D037741:Fullerenes; D014475:Uncoupling Agents; D014867:Water
Mitochondrial transmembrane potential; Uncouplers of oxidative phosphorylation; Water-soluble methanofullerenes