Gene Expression of Col11A1 Is a Marker Not only for Pancreas Carcinoma But also for Adenocarcinoma of the Papilla of Vater, Discriminating Between Carcinoma and Chronic Pancreatitis.
OBJECTIVE
For tumors of the periampullary region clinical differentiation between primary and tumor-associated pancreatitis might be difficult. Early diagnosis of these malignancies is essential, as they present with early invasion of surrounding tissue thus limiting therapeutic options. Using molecular markers, the preoperative diagnosis (EUS-guided needle biopsy, brush biopsy pancreatic duct) could be optimized and surgical therapy potentially adapted. Alpha1 (XI) collagen Col11A1 is essential for the extracellular matrix and normal skeletal development and has been associated with carcinogenesis.


METHODS
Forty-three patients with adenocarcinoma of the pancreas, 11 with adenocarcinoma of the papilla of Vater and 23 patients with chronic pancreatitis were included in the study. For all patients mRNA expression of Col11A1 was quantified by TaqMan RT-PCR in tumor or pancreatitis specimen, as well as in the corresponding normal uninvolved tissue and correlated with diagnosis of cancer and chronic pancreatitis.


RESULTS
Col11A1 mRNA expression was 5.25-fold higher in adenocarcinoma of the pancreas (p=0.006) and 8.25-fold in the papilla of Vater (p=0.002) compared to that of chronic pancreatitis specimen.


CONCLUSIONS
Differential mRNA expression of Col11A1 may be applied to preoperatively differentiate between tumors of the periampullary region and chronic pancreatitis and this may potentially have a positive effect on patient survival.
General-, Visceral- and Tumor Surgery, Center for Integrated Oncology (CIO), University Hospital of Cologne, Cologne, Germany.
Robert
R
Kleinert
Marienhausklinikum, Dahlienweg 3, University Hospital of Düsseldorf, Dusseldorf, Germany.
Klaus
K
Prenzel
Department of Surgery, University Hospital of Düsseldorf, Dusseldorf, Germany.
Nikolas
N
Stoecklein
General-, Visceral- and Tumor Surgery, Center for Integrated Oncology (CIO), University Hospital of Cologne, Cologne, Germany.
Hakan
H
Alakus
General-, Visceral- and Tumor Surgery, Center for Integrated Oncology (CIO), University Hospital of Cologne, Cologne, Germany.
Elfriede
E
Bollschweiler
General-, Visceral- and Tumor Surgery, Center for Integrated Oncology (CIO), University Hospital of Cologne, Cologne, Germany.
Arnulf
A
Hölscher
General-, Visceral- and Tumor Surgery, Center for Integrated Oncology (CIO), University Hospital of Cologne, Cologne, Germany ute.warnecke-eberz@uk-koeln.de.
Ute
U
Warnecke-Eberz
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014670:Ampulla of Vater / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics*; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D024161:Collagen Type XI / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D050500:Pancreatitis, Chronic / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C574182:COL11A1 protein, human; D024161:Collagen Type XI; D012333:RNA, Messenger
Col11A1; Pancreatic cancer; cancer of the papilla of vater; chronic pancreatitis; diagnostic marker; mRNA quantification