KA-bridged transplantation of mesencephalic tissue and olfactory ensheathing cells in a Parkinsonian rat model.
The pathology of Parkinson's disease (PD) results mainly from nigrostriatal pathway damage. Unfortunately, commonly used PD therapies do not repair the disconnected circuitry. It has been reported that using kainic acid (KA, an excitatory amino acid) in bridging transplantation may be useful to generate an artificial tract and reconstruct the nigrostriatal pathway in 6-hydroxydopamine (6-OHDA) lesioned rats. In this study, we used KA bridging and a co-graft of rat olfactory ensheathing cells (OECs) and rat E14 embryonic ventral mesencephalic (VM) tissue to restore the nigrostriatal pathway of the PD model rats. The methamphetamine-induced rotational behaviour, 4-[18F]-ADAM (a selectively serotonin transporter radioligand)/micro-PET imaging, and immunohistochemistry were used to assess the effects of the transplantation. At 9 weeks post-grafting in PD model rats, the results showed that the PD rats undergoing VM tissue and OECs co-grafts (VM-OECs) exhibited better motor recovery compared to the rats receiving VM tissue transplantation only. The striatal uptake of 4-[18F]-ADAM and tyrosine hydroxylase immunoreactivity (TH-ir) of the grafted area in the VM-OECs group were also more improved than those of the VM alone group. These results suggested that OECs may enhance the survival of the grafted VM tissue and facilitate the recovery of motor function after VM transplantation. Moreover, OECs possibly promote the elongation of dopaminergic and serotonergic axon in the bridging graft. Copyright © 2015 John Wiley & Sons, Ltd.
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Shao-Ju
SJ
Weng
Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
I-Hsun
IH
Li
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Yuahn-Sieh
YS
Huang
Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Sheau-Huei
SH
Chueh
Department of Nuclear Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Ta-Kai
TK
Chou
Department of Psychiatry, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
San-Yuan
SY
Huang
Department of Nuclear Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Chyng-Yann
CY
Shiue
Department of Nuclear Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Cheng-Yi
CY
Cheng
Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Kuo-Hsing
KH
Ma
D000818:Animals; D008297:Male; D008636:Mesencephalon / Q000637:transplantation*; D009830:Olfactory Bulb / Q000637:transplantation*; D016627:Oxidopamine / Q000009:adverse effects / Q000494:pharmacology; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley
D016627:Oxidopamine
4-[18F]-ADAM; Parkinson's disease; bridging transplantation; mesencephalic tissue; olfactory ensheathing cell; rat model