In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds.
Mesenchymal stem cells (MSCs) have been shown to improve tissue regeneration in several preclinical and clinical trials. These cells have been used in combination with three-dimensional scaffolds as a promising approach in the field of regenerative medicine. We compare the behavior of human adipose-derived MSCs (AdMSCs) on four different biomaterials that are awaiting or have already received FDA approval to determine a suitable regenerative scaffold for delivering these cells to dermal wounds and increasing healing potential. AdMSCs were isolated, characterized, and seeded onto scaffolds based on chitosan, fibrin, bovine collagen, and decellularized porcine dermis. In vitro results demonstrated that the scaffolds strongly influence key parameters, such as seeding efficiency, cellular distribution, attachment, survival, metabolic activity, and paracrine release. Chick chorioallantoic membrane assays revealed that the scaffold composition similarly influences the angiogenic potential of AdMSCs in vivo. The wound healing potential of scaffolds increases by means of a synergistic relationship between AdMSCs and biomaterial resulting in the release of proangiogenic and cytokine factors, which is currently lacking when a scaffold alone is utilized. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their wound healing potential with AdMSCs.
Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany.
Elizabeth A
EA
Wahl
Institute for Regenerative Cures, UC Davis, Sacramento, CA 95817, USA.
Fernando A
FA
Fierro
Department of Biological Sciences, California State University, Sacramento, CA 95819, USA.
Thomas R
TR
Peavy
Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany.
Ursula
U
Hopfner
Institute of Biomedical Engineering, UCL, The Royal Institution, London W1S 4BS, UK.
Julian F
JF
Dye
Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany.
Hans-Günther
HG
Machens
Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany ; FONDAP Center for Genome Regulation, Faculty of Sciences, University of Chile, 7800024 Santiago, Chile.
José T
JT
Egaña
Department of Plastic Surgery and Hand Surgery, University Hospital Klinikum rechts der Isar, Technical University Munich, 81675 Munich, Germany.
Thilo L
TL
Schenck
D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D017768:Skin, Artificial; D054457:Tissue Scaffolds; D014947:Wounds and Injuries / Q000473:pathology* / Q000628:therapy*