Specific detection of peste des petits ruminants virus antibodies in sheep and goat sera by the luciferase immunoprecipitation system.
Peste des petits ruminants (PPR) is a contagious and often fatal transboundary animal disease affecting mostly sheep, goats and wild small ruminants. This disease is endemic in most of Africa, the Middle, Near East, and large parts of Asia. The causal agent is peste des petits ruminants virus (PPRV), which belongs to the genus Morbillivirus in the family Paramyxoviridae. This genus also includes measles virus (MV), canine distemper virus (CDV) and rinderpest virus (RPV). All are closely related viruses with serological cross reactivity. In this study, we have developed a Luciferase Immunoprecipitation System (LIPS) for the rapid detection of antibodies against PPRV in serum samples and for specific differentiation from antibodies against RPV. PPR and rinderpest (RP) serum samples were assayed by PPR-LIPS and two commercially available PPR cELISA tests. The PPR-LIPS showed high sensitivity and specificity for the samples tested and showed no cross reactivity with RPV unlike the commercial PPR cELISA tests which did cross react with RPV. Based on the results shown in this study, PPR-LIPS is presented as a good candidate for the specific serosurveillance of PPR.
Animal Production and Health Laboratory, FAO/IAEA Agriculture and & Biotechnology Laboratory, IAEA Laboratories Seibersdorf, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria. Electronic address: f.berguido@iaea.org.
Francisco J
FJ
Berguido
Pan African Veterinary Vaccine Center of the African Union (AU-PANVAC), P.O Box 1746, Debre-Zeit, Ethiopia.
Sanne Charles
SC
Bodjo
Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety, Moedling, Austria (AGES).
Angelika
A
Loitsch
Animal Production and Health Laboratory, FAO/IAEA Agriculture and & Biotechnology Laboratory, IAEA Laboratories Seibersdorf, International Atomic Energy Agency (IAEA), Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria.
Adama
A
Diallo
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006041:Goats / Q000097:blood / Q000276:immunology* / Q000821:virology; D047468:Immunoprecipitation; D008156:Luciferases; D029021:Peste-des-Petits-Ruminants / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000276:immunology*; D012302:Rinderpest virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012756:Sheep / Q000097:blood / Q000276:immunology* / Q000821:virology
D000914:Antibodies, Viral; D008156:Luciferases