Stochastic Optical Reconstruction Microscopy Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots.
Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the current study, stochastic optical reconstruction microscopy (STORM) imaging of intact Arabidopsis thaliana seedling roots with a spatial resolution of 20-40 nm was demonstrated. Using the super-resolution images, the spatial organization of cortical microtubules in different parts of a whole Arabidopsis root tip was analyzed quantitatively, and the results show the dramatic differences in the density and spatial organization of cortical microtubules in cells of different differentiation stages or types. The method developed can be applied to plant cell biological processes, including imaging of additional elements of the cytoskeleton, organelle substructure, and membrane domains.
Ames Laboratory, US Department of Energy and Department of Chemistry, Iowa State University, Ames, Iowa 50011.
Bin
B
Dong
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011.
Xiaochen
X
Yang
Ames Laboratory, US Department of Energy and Department of Chemistry, Iowa State University, Ames, Iowa 50011.
Shaobin
S
Zhu
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa 50011.|Plant Sciences Institute, Iowa State University, Ames, Iowa 50011.
Diane C
DC
Bassham
Department of Chemistry, Georgia State University, P.O. Box 3965, Atlanta, Georgia 30302.
Ning
N
Fang
D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism; D018519:Meristem / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism
D029681:Arabidopsis Proteins