Decreased Phototoxic Effects of TiO₂ Nanoparticles in Consortium of Bacterial Isolates from Domestic Waste Water.
This study is aimed to explore the toxicity of TiO2 nanoparticles at low concentrations (0.25, 0.50 & 1.00 μg/ml); on five bacterial isolates and their consortium in waste water medium both in dark and UVA conditions. To critically examine the toxic effects of nanoparticles and the response mechanism(s) offered by microbes, several aspects were monitored viz. cell viability, ROS generation, SOD activity, membrane permeability, EPS release and biofilm formation. A dose and time dependent loss in viability was observed for treated isolates and the consortium. At the highest dose, after 24h, oxidative stress was examined which conclusively showed more ROS generation & cell permeability and less SOD activity in single isolates as compared to the consortium. As a defense mechanism, EPS release was enhanced in case of the consortium against the single isolates, and was observed to be dose dependent. Similar results were noticed for biofilm formation, which substantially increased at highest dose of nanoparticle exposure. Concluding, the consortium showed more resistance against the toxic effects of the TiO2 nanoparticles compared to the individual isolates.
Centre for Nanobiotechnology, VIT University, Vellore, India.
Ankita
A
Mathur
Centre for Nanobiotechnology, VIT University, Vellore, India.
Jyoti
J
Kumari
Centre for Nanobiotechnology, VIT University, Vellore, India.
Abhinav
A
Parashar
Centre for Nanobiotechnology, VIT University, Vellore, India.
Lavanya
L
T
Centre for Nanobiotechnology, VIT University, Vellore, India.
N
N
Chandrasekaran
Centre for Nanobiotechnology, VIT University, Vellore, India.
Amitava
A
Mukherjee
D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D018441:Biofilms; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D059013:Microbial Consortia / Q000187:drug effects*; D050296:Microbial Viability; D018384:Oxidative Stress; D010316:Particle Size; D055668:Photochemical Processes; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000494:pharmacology*; D018508:Water Purification
D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D014025:Titanium; D062065:Wastewater; D014874:Water Pollutants, Chemical; C009495:titanium dioxide