Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces.
Ordered and reproducible bioprobe immobilization onto sensor surfaces is a critical step in the development of reliable analytical devices. A growing awareness of the impact of the immobilization scheme on the consistency of the generated data is driving the demand for chemoselective approaches to immobilize biofunctional ligands, such as peptides, in a predetermined and uniform fashion. Herein, the most intriguing strategies to selective and oriented peptide immobilization are described and discussed. The aim of the current work is to provide the reader a general picture on recent advances made in this field, highlighting the potential associated with each chemoselective strategy. Case studies are described to provide illustrative examples, and cross-references to more topic-focused and exhaustive reviews are proposed throughout the text.
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy. alessandro.gori@icrm.cnr.it.
Alessandro
A
Gori
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Renato
R
Longhi
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D015335:Molecular Probes / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D055767:Immobilized Proteins; D015335:Molecular Probes; D010091:Oximes; D010455:Peptides; D011506:Proteins; D013438:Sulfhydryl Compounds
Chemoselective reactions; Click chemistry; Oriented immobilization; Peptide microarray