Fluorescence-Guided Probes of Aptamer-Targeted Gold Nanoparticles with Computed Tomography Imaging Accesses for in Vivo Tumor Resection.
Recent development of molecular imaging probes for fluorescence-guided surgery has shown great progresses for determining tumor margin to execute the tissue resection. Here we synthesize the fluorescent gold nanoparticles conjugated with diatrizoic acid and nucleolin-targeted AS1411 aptamer. The nanoparticle conjugates exhibit high water-solubility, good biocompatibility, visible fluorescence and strong X-ray attenuation for computed tomography (CT) contrast enhancement. The fluorescent nanoparticle conjugates are applied as a molecular contrast agent to reveal the tumor location in CL1-5 tumor-bearing mice by CT imaging. Furthermore, the orange-red fluorescence emitting from the conjugates in the CL1-5 tumor can be easily visualized by the naked eyes. After the resection, the IVIS measurements show that the fluorescence signal of the nanoparticle conjugates in the tumor is greatly enhanced in comparison to that in the controlled experiment. Our work has shown potential application of functionalized nanoparticles as a dual-function imaging agent in clinical fluorescence-guided surgery.
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Cheng-Hung
CH
Li
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.|Ph. D. Program in Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Tsung-Rong
TR
Kuo
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Hsin-Jan
HJ
Su
Institute of Biomedical Sciences, Academia Sinica, Taipei 10617, Taiwan.
Wei-Yun
WY
Lai
Institute of Biomedical Sciences, Academia Sinica, Taipei 10617, Taiwan.|Division of Pulmonary Medicine, Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, Taipei 10051, Taiwan.
Pan-Chyr
PC
Yang
Division of Colorectal Surgery, Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33302, Taiwan.
Jinn-Shiun
JS
Chen
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.
Di-Yan
DY
Wang
Institute of Molecular and Cell Biology, National Taiwan University, Taipei, 10617, Taiwan.|Institute of Atomic and Molecular Science, Academia Sinica, Taipei 10617, Taiwan.
Yi-Chun
YC
Wu
Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan.|Institute of Atomic and Molecular Science, Academia Sinica, Taipei 10617, Taiwan.
Chia-Chun
CC
Chen
D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000008:administration & dosage*; D006046:Gold / Q000008:administration & dosage*; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D057054:Molecular Imaging / Q000379:methods; D015335:Molecular Probes / Q000008:administration & dosage*; D009369:Neoplasms / Q000473:pathology*; D014057:Tomography, X-Ray Computed / Q000379:methods
D003287:Contrast Media; D005456:Fluorescent Dyes; D015335:Molecular Probes; D006046:Gold