Anti-inflammatory effects of potato extract on a rat model of cigarette smoke-induced chronic obstructive pulmonary disease.
OBJECTIVE
This study aimed to evaluate the therapeutic effects of potato extract (PE) on cigarette smoke (CS)-induced chronic obstructive pulmonary disease (COPD).


METHODS
PE was first prepared by frozen centrifugation, and its amino acid composition was detected. Toxicity of PE was analyzed by changes in morphology, behavior, routine blood indexes, and biochemical criteria of mice. Then, the COPD rat model was established by CS exposure, and PE, doxofylline, and prednisolone acetate were used to treat these rats. After 45 days of treatment, the morphology and behavior of rats were recorded. In addition, the histopathology of lung tissue was evaluated by chest x-ray and hematoxylin and eosin staining. The expression of interleukine-10 (IL-10), tumor necrosis factor-α (TNF-α), and granulocyte colony-stimulating factor (G-CSF) was detected in serum and lung tissue by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, respectively.


RESULTS
Various amino acids were identified in PE, and no toxicity was exhibited in mice. The CS-induced COPD rat model was successfully established, which exhibited significant thickened and disordered lung markings on 90% of the rats. After administering doxofylline and prednisolone acetate, inflammation symptoms were improved. However, side effects such as emaciation, weakness, and loosening of teeth appeared. In the PE group, obviously improved histopathology was observed in lung tissues. Meanwhile, it was revealed that PE could increase the expression of IL-10 and reduce the expression of TNF-α and G-CSF in COPD rats, and doxofylline and prednisolone acetate also elicited similar results.


CONCLUSIONS
Our study suggests PE might be effective in the treatment of CS-induced COPD by inhibiting inflammation.
Department of Clinical Medical Research Center, Inner Mongolia Autonomous Region People's Hospital, Hohhot, Inner Mongolia, China.
Gui Hua
GH
Xu
Department of Neurology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, Inner Mongolia, China.
Jie
J
Shen
Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Peng
P
Sun
Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.|Inner Mongolia Mengjian Biotechnology company, Wuchua, Inner Mongolia, China; nmg_ylm@163.com.
Min Li
ML
Yang
Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China; pengwzhao@126.com.
Peng Wei
PW
Zhao
Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Yan
Y
Niu
Institute of Microbiology and Immunology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Jing Kun
JK
Lu
Department of Anatomy, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Zhi Qiang
ZQ
Wang
Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Chao
C
Gao
Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Xue
X
Han
Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Lei Lei
LL
Liu
Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Chen Chen
CC
Liu
Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Zhang Yue
ZY
Cong
chronic obstructive pulmonary disease; cigarette smoke; inflammation; potato extract