HEA BeadChip™ technology in immunohematology.
Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing. Thanks to increased knowledge of the molecular basis associated with many blood group antigens, it is currently possible to predict their presence or absence on the red cell membrane. Several molecular techniques have been developed to detect the most important allelic variations attributable to single nucleotide polymorphisms. The human erythrocyte antigen (HEA) BeadChip™ system manufactured by BioArray Solutions (Immucor, Warren, NJ) is one of the commercial DNA array platforms currently available to predict HEAs by DNA analysis. This technology provides a useful tool to increase the inventory of antigen-negative RBC units and prevent immunization of patients who require chronic transfusion by providing compatible RBC units based on matching by DNA testing.
ScD, Senior Assistant Biologist (corresponding author), Immunohematology Reference Laboratory (IRL), Transfusion Center, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Cinzia
C
Paccapelo
ScD, Senior Technician, IRL, Transfusion Center, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Francesca
F
Truglio
ScD, Technical Supervisor, IRL, Transfusion Center, Foundation Ca' Granda Maggiore Ospedale Policlinico, Milan, Italy.
Maria
M
Antonietta Villa
ScD, Technical Supervisor, IRL, Transfusion Center, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Nicoletta
N
Revelli
MD, Director Transfusion Center, Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Maurizio
M
Marconi
D000483:Alleles; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology; D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000097:blood / Q000235:genetics; D017707:Erythrocyte Transfusion / Q000379:methods; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism*; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics* / Q000276:immunology; D015203:Reproducibility of Results
D001789:Blood Group Antigens; D004247:DNA