All-plastic miniature fluorescence microscope for point-of-care readout of bead-based bioassays.
A number of new platforms have been developed for multiplexed bioassays that rely on imaging targeted fluorescent beads labeled with different fluorescent dyes. We developed a compact, low-cost three-dimensional printed fluorescence microscope that can be used as a detector for mutiplexed, bead-based assays to support point-of-care applications. Images obtained with the microscope were analyzed to differentiate multiple analytes in a single sample with a comparable limit of detection to commercially available macroscopic assay platforms.
Alessandra
A
Forcucci
Michal Emanuel
ME
Pawlowski
Zachary
Z
Crannell
Ina
I
Pavlova
Rebecca
R
Richards-Kortum
Tomasz S
TS
Tkaczyk
D001681:Biological Assay / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D008904:Miniaturization; D000067716:Point-of-Care Testing; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity