Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.
The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.
Division of Physical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. aleksandra.dabkowska@fkem1.lu.se and NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se.
Aleksandra P
AP
Dabkowska
NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se and Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Gaëlle
G
Piret
NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se and Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Cassandra S
CS
Niman
NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se and Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Mercy
M
Lard
NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se and Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Heiner
H
Linke
Division of Physical Chemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. aleksandra.dabkowska@fkem1.lu.se and NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se.
Tommy
T
Nylander
NanoLund, Lund University, P.O. Box 118, SE-22100 Lund, Sweden. christelle.prinz@ftf.lth.se and Division of Solid State Physics, Lund University, P.O. Box 118, SE-22100 Lund, Sweden.
Christelle N
CN
Prinz
D005456:Fluorescent Dyes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*
D005456:Fluorescent Dyes; D008051:Lipid Bilayers