A fusant of Amycolatopsis sp. M3-1 and Pseudomonas sp. Nai8 with high capacity of degrading novel pyrimidynyloxybenzoic herbicide ZJ0273 and naphthalene.
ZJ0273 (propyl 4-(2-(4, 6-demethoxy pyrimidin-2-yloxy) benzylamino) benzoate) is a novel pyrimidynyloxybenzoic-based herbicide developed in China for oilseed crop. This study was aimed to construct new strains capable of degrading naphthalene and ZJ0273 by protoplast fusion between Amycolatopsis sp. M3-1 and Pseudomonas sp. Nai8. Eight recombinant strains were successfully produced, and the strains could simultaneously utilize ZJ0273 and naphthalene as the sole carbon and energy source, respectively. One of recombinant strains, MN6 with higher degrading efficiency, was chosen for further study. Under the condition of pH 7.0, 30 °C, ZJ0273 and naphthalene degradation percent by the recombinant strain MN6 could reach 65.10% (20 days) and 88.46% (48 h), respectively. According to the identified six metabolites (M1-M6) by LC-MS/MS, biodegradation pathway of ZJ0273 was proposed. ZJ0273 biodegradation catalyzed by the recombinant strain MN6 involved continuous biocatalytic reactions such as de-estering, hydrolysis, acylation, C-N cleavage, de-methyl, and ether cleavage reactions.
Department of Pharmacy, Medical College of Qinghai University, Qinghai, Xining, 810001, China.
Xiaohong
X
Chen
Laboratory of Applied Microbiology and Biotechnology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China. zhqcai@cczu.edu.cn.
Zhiqiang
http://orcid.org/0000-0002-9180-675X
Z
Cai
D039903:Actinobacteria / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D001565:Benzoates / Q000032:analysis*; D001673:Biodegradation, Environmental; D002681:China; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry; D006540:Herbicides / Q000032:analysis* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009281:Naphthalenes / Q000032:analysis* / Q000737:chemistry; D011523:Protoplasts / Q000648:ultrastructure; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D053719:Tandem Mass Spectrometry
D001565:Benzoates; D004785:Environmental Pollutants; D006540:Herbicides; D009281:Naphthalenes; C543844:propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino)benzoate; C031721:naphthalene
Biodegradation; Degradation pathway; Naphthalene; Protoplast fusion; ZJ0273