Characterization of Staphylococcus caprae Clinical Isolates Involved in Human Bone and Joint Infections, Compared with Goat Mastitis Isolates.
Staphylococcus caprae is an emerging microorganism in human bone and joint infections (BJI). The aim of this study is to describe the features of S. caprae isolates involved in BJI (H for human) compared with those of isolates recovered in goat mastitis (A for animal). Fourteen isolates of each origin were included. Identifications were performed using a Vitek 2 GP ID card, tuf gene sequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) Vitek MS. Molecular typing was carried out using pulsed-field gel electrophoresis (PFGE) and DiversiLab technology. The crystal violet method was used to determine biofilm-forming ability. Virulence factors were searched by PCR. Vitek MS technology provides an accurate identification for the two types of isolates compared to that of gold-standard sequencing (sensitivity, 96.4%), whereas the Vitek 2 GP ID card was more effective for H isolates. Molecular typing methods revealed two distinct lineages corresponding to the origin despite few overlaps: H and A. In our experimental conditions, no significant difference was observed in biofilm production ability between H and A isolates. Nine isolates (5 H isolates and 4 A isolates) behaved as weak producers while one A isolate was a strong producer. Concerning virulence factors, the autolysin atlC and the serine aspartate adhesin (sdrZ) genes were detected in 24 isolates (86%), whereas the lipase gene was always detected, except in one H isolate (96%). The ica operon was present in 23 isolates (82%). Fibrinogen-binding (fbe) or collagen-binding (cna) genes were not detected by using primers designed for Staphylococcus aureus or Staphylococcus epidermidis, even in low stringency conditions. Although S. caprae probably remains underestimated in human infections, further studies are needed to better understand the evolution and the adaptation of this species to its host.
Service de Bactériologie-Hygiène hospitalière, CHU de Nantes, Nantes, France.
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d'Ersu
Service de Bactériologie-Hygiène hospitalière, CHU de Nantes, Nantes, France EA3826 Thérapeutiques Cliniques et Expérimentales des Infections, Faculté de Médecine, Nantes, France Nantes study group members of CRIOGO, Centre de Référence des Infections Ostéo-articulaires du Grand Ouest, Nantes, France.
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Aubin
ANSES de Niort, Niort, France.
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Mercier
Laboratoire d'Analyses Sèvres Atlantique, Niort, France.
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Nicollet
Service de Bactériologie-Hygiène hospitalière, CHU de Nantes, Nantes, France Nantes study group members of CRIOGO, Centre de Référence des Infections Ostéo-articulaires du Grand Ouest, Nantes, France.
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Bémer
Service de Bactériologie-Hygiène hospitalière, CHU de Nantes, Nantes, France EA3826 Thérapeutiques Cliniques et Expérimentales des Infections, Faculté de Médecine, Nantes, France Nantes study group members of CRIOGO, Centre de Référence des Infections Ostéo-articulaires du Grand Ouest, Nantes, France stephane.corvec@chu-nantes.fr.
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http://orcid.org/0000-0003-3176-8111
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Corvec
D000328:Adult; D000368:Aged; D000818:Animals; D018441:Biofilms / Q000254:growth & development; D005260:Female; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D006801:Humans; D008297:Male; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008875:Middle Aged; D058889:Molecular Typing; D010003:Osteoarthritis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary*; D013210:Staphylococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult