Chronic social defeat stress increases dopamine D2 receptor dimerization in the prefrontal cortex of adult mice.
The present study aimed to examine the effects of chronic social defeat stress on the dopamine receptors and proteins involved in post-endocytic trafficking pathways. Adult mice were divided into susceptible and unsusceptible groups after 10 days of social defeat stress. Western blot analysis was used to measure the protein expression levels of dopamine D2 receptors (D2Rs), a short (D2S) and a long form (D2L) and, D2R monomers and dimers, dopamine D1 receptors (D1Rs), neuronal calcium sensor-1 (NCS-1) and G protein-coupled receptor-associated sorting protein-1 (GASP-1), and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of D2S, D2L, D2R monomers and dimers, and D1Rs in different brain areas. We observed increased expression of D2S, D2L and D2Rs dimers in the prefrontal cortex (PFC) of susceptible and/or unsusceptible mice compared with controls. The only significant findings with regard to mRNA expression levels were lower expression of D2S mRNA in the amygdala (AMYG) of susceptible and unsusceptible mice compared with controls. The present study demonstrated that chronic social defeat stress induced increased expression of D2S, D2L, and D2R dimers in the PFC of susceptible and/or unsusceptible mice.
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea.
T R
TR
Bagalkot
Department of Physiology, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea.
H-M
HM
Jin
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea.
V V
VV
Prabhu
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea.
S S
SS
Muna
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea.
Y
Y
Cui
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea.
B K
BK
Yadav
Department of Pharmacology, Chonbuk National University Medical School and Institute of Medical Sciences, Republic of Korea.
H-J
HJ
Chae
Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: chungyc@jbnu.ac.kr.
Y-C
YC
Chung
D000679:Amygdala / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D002908:Chronic Disease; D003342:Corpus Striatum / Q000378:metabolism; D019281:Dimerization; D004195:Disease Models, Animal; D004291:Dominance-Subordination; D006624:Hippocampus / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008810:Mice, Inbred C57BL; D051580:Neuronal Calcium-Sensor Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017397:Prefrontal Cortex / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D055500:Resilience, Psychological; D013315:Stress, Psychological / Q000378:metabolism*
D002352:Carrier Proteins; C000600618:DRD2 protein, mouse; C583261:Drd1 protein, mouse; C479636:Gprasp1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; C101464:frequenin calcium sensor proteins
G protein-coupled receptor-associated sorting protein-1; dopamine D(2) receptor dimers; dopamine receptor isoforms; neuronal calcium sensor-1; social defeat