A Comparison of the Process of Remodeling of Hydroxyapatite/Poly-D/L-Lactide and Beta-Tricalcium Phosphate in a Loading Site.
Currently, the most commonly used bioresorbable scaffold is made of beta-tricalcium phosphate (β-TCP); it is hoped that scaffolds made of a mixture of hydroxyapatite (HA) and poly-D/L-lactide (PDLLA) will be able to act as novel bioresorbable scaffolds. The aim of this study was to evaluate the utility of a HA/PDLLA scaffold compared to β-TCP, at a loading site. Dogs underwent surgery to replace a section of tibial bone with a bioresorbable scaffold. After the follow-up period, the scaffold was subjected to histological analysis. The HA/PDLLA scaffold showed similar bone formation and superior cell and tissue infiltration compared to the β-TCP scaffold, as seen after Villanueva Goldner staining. Moreover, silver staining and immunohistochemistry for Von Willebrand factor and cathepsin K demonstrated better cell infiltration in the HA/PDLLA scaffold. The fibrous tissue and cells that had infiltrated into the HA/PDLLA scaffold tested positive for collagen type I and RUNX2, respectively, indicating that the tissue and cells that had infiltrated into the HA/PDLLA scaffold had the potential to differentiate into bone. The HA/PDLLA scaffold is therefore likely to find clinical application as a new bioresorbable scaffold.
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Hiroyuki
H
Akagi
Division of Veterinary Microbiology, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Hiroki
H
Ochi
Division of Veterinary Anatomy, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Satoshi
S
Soeta
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Nobuo
N
Kanno
Medical Research Group, Development Department, Takiron Co., Ltd., 7-1-19, Minatojima Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Megumi
M
Yoshihara
Medical Research Group, Development Department, Takiron Co., Ltd., 7-1-19, Minatojima Minamimachi, Chuo-ku, Kobe 650-0047, Japan.
Kenshi
K
Okazaki
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Takuya
T
Yogo
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Yasuji
Y
Harada
Division of Veterinary Anatomy, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Hajime
H
Amasaki
Division of Veterinary Surgery, Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Musashino, Tokyo 180-8602, Japan.
Yasushi
Y
Hara
D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000032:analysis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004285:Dogs; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters / Q000737:chemistry*; D013977:Tibia / Q000737:chemistry / Q000293:injuries / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry*
D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; C485817:beta-tricalcium phosphate; C033616:poly(lactide); D017886:Durapatite