Encapsulation of pancreatic islet with HMGB1 fragment for attenuating inflammation.
BACKGROUND
Pancreatic islet encapsulation is one way to address the disadvantages of islet transplantation. Not only does encapsulation involve bidirectional diffusion of nutrients, oxygen, and glucose, but also it protects the graft from the recipient's immune reaction. The high mobility group box 1 (HMGB1), one of higher expression proteins in islet, can be secreted from transplanted islets and induce the inflammation. Therefore, the regulation of HMGB1-mediated inflammation is very important for successful islet transplantation. In this study, we used the HMGB1 A box, an antagonist of HMGB1 receptor in the immune cells, in the encapsulation of isolated islets as a new strategy.


RESULTS
For co-encapsulation of HMGB1 A box protein with islets, we evaluated the distribution of alginate bead diameter. The average diameter of empty alginate bead was similar to that of alginate bead with islets. When different concentrations of HMGB1 A box protein was co-encapsulated with islets, it did not affect the viability and insulin secretion function of the islets. When the alginate beads with islets plus HMGB1 A box protein were cultured with macrophage, the amount of TNF-α secreted from the macrophages was significantly attenuated when compared to cultivation of unencapsulated islets or encapsulated islets. When the alginate beads with islets plus HMGB1 A box protein were intraperitoneally xenotransplanted into the diabetic mice, the survival rate of the islets was strongly improved with 2-fold.


CONCLUSIONS
Collectively, these results suggested that the encapsulation of HMGB1 A box protein might offer a protective effect in islet transplantation.
Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-791 Republic of Korea.|BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791 Republic of Korea.
Eun Hee
EH
Jo
Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-791 Republic of Korea.|BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791 Republic of Korea.
Yong Hwa
YH
Hwang
Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-791 Republic of Korea.|BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 133-791 Republic of Korea.
Dong Yun
DY
Lee
Alginate bead; Encapsulation; HMGB1 A box; High mobility group box 1 (HMGB1); Pancreatic islet transplantation