An integrated functional genomic analysis identifies the antitumorigenic mechanism of action for PPARγ in lung cancer cells.
Integrating the analysis of the cistrome of a transcription factor by ChIP-Seq with the study of its transcriptional output by microarray or RNA-Seq analysis is a powerful approach to elucidate the genomic functions of a transcription factor. Recently, we employed this approach to determine the mechanism of action by which the nuclear receptor PPARγ elicits its antitumorigenic effects in lung cancer cells upon activation by TZDs (1). Here we describe in detail the design, contents and quality controls for the gene expression and cistrome analyses associated with our study published in Cell Metabolism in 2014.
Eugene McDermott Center for Human Growth and Development, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Rahul K
RK
Kollipara
Eugene McDermott Center for Human Growth and Development, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA ; Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA ; Department of Pharmacology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA ; Green Center for Reproductive Biology Sciences, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Ralf
R
Kittler
Bioinformatics; ChIP-Seq; Gene expression; Lung cancer; Microarray