Effects of Soothing Liver and Invigorating Spleen Recipes on the IKKβ-NF-κB Signaling Pathway in Kupffer Cells of Nonalcoholic Steatohepatitis Rats.
This study investigates the effect of soothing liver and invigorating spleen recipes on steatohepatitis examining the IKKβ-NF-κB signaling pathway in KCs of NASH rats. SD male rats were randomly divided into 8 groups, and the NASH model was induced by a high-fat diet (HFD). After 26 weeks, liver tissue was examined in H&E stained sections and liver function was monitored biochemically. KCs were isolated by Seglen's method, with some modifications. The mRNA and protein expression of the IKKβ-NF-κB signaling pathway components was examined by quantitative PCR and Western blotting. The results show that the high-fat diet induced NASH in the rats, and the soothing liver recipe and invigorating spleen recipe decreased the levels of TNF-α, IL-1, and IL-6 in KCs, as well as inhibiting the mRNA and protein expression of the IKKβ-NF-κB signaling pathway components. In conclusion, the experiment indicated the importance of the IKKβ-NF-κB signaling pathway in KCs for the anti-inflammatory effects of the soothing liver and invigorating spleen recipes.
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Xiang-Wen
XW
Gong
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Yong-Jian
YJ
Xu
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Qin-He
QH
Yang
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Yin-Ji
YJ
Liang
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Yu-Pei
YP
Zhang
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Guan-Long
GL
Wang
Department of Traditional Chinese Medicine, Medical College of Jinan University, 601 Huangpu Road West, Guangzhou, Guangdong 510632, China.
Yuan-Yuan
YY
Li