Biosorption of Pb(II) Ions by Klebsiella sp. 3S1 Isolated from a Wastewater Treatment Plant: Kinetics and Mechanisms Studies.
Lead biosorption by Klebsiella sp. 3S1 isolated from a wastewater treatment plant was investigated through a Rotatable Central Composite Experimental Design. The optimisation study indicated the following optimal values of operating variables: 0.4 g/L of biosorbent dosage, pH 5, and 34°C. According to the results of the kinetic studies, the biosorption process can be described by a two-step process, one rapid, almost instantaneous, and one slower, both contributing significantly to the overall biosorption; the model that best fits the experimental results was pseudo-second order. The equilibrium studies showed a maximum lead uptake value of 140.19 mg/g according to the Langmuir model. The mechanism study revealed that lead ions were bioaccumulated into the cytoplasm and adsorbed on the cell surface. The bacterium  Klebsiella sp. 3S1 has a good potential in the bioremoval of lead in an inexpensive and effective process.
Department of Chemical, Environmental and Materials Engineering, University of Jaén, 23071 Jaén, Spain.
Antonio Jesús
AJ
Muñoz
Department of Chemical, Environmental and Materials Engineering, University of Jaén, 23071 Jaén, Spain.
Francisco
F
Espínola
Department of Chemical, Environmental and Materials Engineering, University of Jaén, 23071 Jaén, Spain.
Manuel
M
Moya
Department of Chemical, Environmental and Materials Engineering, University of Jaén, 23071 Jaén, Spain.
Encarnación
E
Ruiz
D001673:Biodegradation, Environmental; D007709:Klebsiella / Q000302:isolation & purification / Q000378:metabolism*; D007854:Lead / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D008962:Models, Theoretical; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D007854:Lead; D062065:Wastewater; D014874:Water Pollutants, Chemical