Defying death: Cellular survival strategies following plasmalemmal injury by bacterial toxins.
The perforation of the plasmalemma by pore-forming toxins causes an influx of Ca(2+) and an efflux of cytoplasmic constituents. In order to ensure survival, the cell needs to identify, plug and remove lesions from its membrane. Quarantined by membrane folds and isolated by membrane fusion, the pores are removed from the plasmalemma and expelled into the extracellular space. Outward vesiculation and microparticle shedding seem to be the strategies of choice to eliminate toxin-perforated membrane regions from the plasmalemma of host cells. Depending on the cell type and the nature of injury, the membrane lesion can also be taken up by endocytosis and degraded internally. Host cells make excellent use of an initial, moderate rise in intracellular [Ca(2+)], which triggers containment of the toxin-inflicted damage and resealing of the damaged plasmalemma. Additional Ca(2+)-dependent defensive cellular actions range from the release of effector molecules in order to warn neighbouring cells, to the activation of caspases for the initiation of apoptosis in order to eliminate heavily damaged, dysregulated cells. Injury to the plasmalemma by bacterial toxins can be prevented by the early sequestration of bacterial toxins. Artificial liposomes can act as a decoy system preferentially binding and neutralizing bacterial toxins.
Institute of Anatomy, University of Bern, Switzerland.
Eduard B
EB
Babiychuk
Institute of Anatomy, University of Bern, Switzerland. Electronic address: draeger@ana.unibe.ch.
Annette
A
Draeger
D000818:Animals; D017302:Annexins / Q000502:physiology; D001427:Bacterial Toxins / Q000494:pharmacology*; D020013:Calcium Signaling; D002462:Cell Membrane / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D055252:Cell-Derived Microparticles / Q000502:physiology; D004705:Endocytosis; D006801:Humans
D017302:Annexins; D001427:Bacterial Toxins
Annexins; Blebbing; Ca(2+); Microparticle; Microvesicle; P2X7 receptor; Plasma membrane repair