Comparison of medetomidine, thiopental and ketamine/midazolam anesthesia in chick embryos for in ovo Magnetic Resonance Imaging free of motion artifacts.
Non-invasive assessment of the perfusion capacity of tissue engineered constructs grown on the chorioallantoic membrane by MRI is often hampered by motion artifacts. Therefore, we examined the suitability of three anesthetic regimes for sufficient sedation of the chick embryo. Medetomidine at a dosage of 0.3 mg/kg, was compared to thiopental at 100 mg/kg and ketamine/midazolam at 50 mg/kg and 1 mg/kg, respectively. These soluble anesthetics were applied by dropping a total volume of 0.3 mL onto the surface of the CAM. Motion was videotaped through the window of the eggshell and scored semi-quantitatively. Medetomidine performed best in terms of reduced motion; onset of anesthesia occurred within 10 minutes and for the following 30 minutes, allowing proper in vivo MRI measurements. The other regimen were not sedating deep enough (ketamine/midazolam) and not long enough (thiopental). In sum, medetomidine allows proper sedation for MRI assessment of the perfusion capacity in a tissue engineered construct placed on the CAM.
Institute for Biomedical Engineering, ETH and University of Zurich, HIT E22.3 Wolfgang-Pauli-Str. 27, 8093 Zurich, Switzerland.|Visceral and Transplant Surgery, University Hospital Zurich, Sternwartstr. 14, 8091 Zurich, Switzerland.
Conny
C
Waschkies
BZL, Biological Central Laboratory, University Hospital Zurich, Sternwartstrasse 6, 8091 Zurich, Switzerland.
Flora
F
Nicholls
Plastic Surgery and Hand Surgery, University Hospital Zurich, Sternwartstr. 14, 8091 Zurich, Switzerland.
Johanna
J
Buschmann
D000758:Anesthesia; D000818:Animals; D002642:Chick Embryo; D007649:Ketamine / Q000008:administration & dosage*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D020926:Medetomidine / Q000008:administration & dosage*; D008874:Midazolam / Q000008:administration & dosage*; D013874:Thiopental / Q000008:administration & dosage*
D007649:Ketamine; D013874:Thiopental; D020926:Medetomidine; D008874:Midazolam