Core Needle Biopsy Wash Optimization: Enabling Specimen Integrity for both Cytological and Histological Evaluation.
BACKGROUND
The recovery of cells after washing core needle biopsies represents an under-utilized approach to extend the diagnostic capacity of these diminutive specimens. Recovery of these cells can be dedicated for molecular studies so that the biopsy itself can be used apropos for its intended purpose, diagnosis. Non-enzymatic and enzymatic reagents have the potential to increase the number of cells dissociating from the tissue core, but can also negatively impact the quality of the tissue itself.


METHODS
Three different means (phosphate-buffered saline, a non-enzymatic and an enzymatic solution) were used to wash core needle biopsies. The washed cells were recovered by traditional preparatory methods and evaluated for cellularity and cytomorphology. The post-washed cores were processed by formalin fixation, paraffin embedding and evaluated for integrity and morphological quality.


RESULTS
The enzymatic solution damaged both the cytological and tissue specimens, while the saline and non-enzymatic process allowed for the comparable recovery of cells and tissue for evaluation.


CONCLUSIONS
Adequate numbers of cells are dissociated from the tissue core when needle biopsies are washed. The recovery and preservation of cells and tissue for morphological interpretation was optimal when solutions devoid of enzymes were used for washing.
Department of Pathology and Anatomical Sciences, State University at Buffalo, Buffalo, NY, U.S.A. mojica@buffalo.edu.
Wilfrido D
WD
Mojica
Department of Pathology and Anatomical Sciences, State University at Buffalo, Buffalo, NY, U.S.A.
Nora K
NK
Frisch
Department of Pathology and Anatomical Sciences, State University at Buffalo, Buffalo, NY, U.S.A.
Joyce K
JK
Paterson
D062005:Biopsy, Large-Core Needle / Q000379:methods* / Q000592:standards*; D003584:Cytological Techniques; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000601:surgery; D016612:Paraffin Embedding; D013048:Specimen Handling / Q000379:methods*; D016707:Tissue Fixation
Core needle biopsy; PBS; core needle wash; cytology; microfluidics