Pore-forming toxins: Properties, diversity, and uses as tools to image sphingomyelin and ceramide phosphoethanolamine.
Pore-forming toxins (PFTs) represent a unique class of highly specific lipid-binding proteins. The cytotoxicity of these compounds has been overcome through crystallographic structure and mutation studies, facilitating the development of non-toxic lipid probes. As a consequence, non-toxic PFTs have been utilized as highly specific probes to visualize the diversity and dynamics of lipid nanostructures in living and fixed cells. This review is focused on the application of PFTs and their non-toxic analogs as tools to visualize sphingomyelin and ceramide phosphoethanolamine, two major phosphosphingolipids in mammalian and insect cells, respectively. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale.
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
Akiko
A
Yamaji-Hasegawa
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; INSERM U1060, Université Lyon 1, Villeurbanne, 69621, France.
Françoise
F
Hullin-Matsuda
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
Peter
P
Greimel
Lipid Biology Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; INSERM U1060, Université Lyon 1, Villeurbanne, 69621, France. Electronic address: kobayasi@riken.jp.
Toshihide
T
Kobayashi
D000818:Animals; D006801:Humans; D007313:Insecta / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry*; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry*; D013109:Sphingomyelins / Q000378:metabolism*
D015335:Molecular Probes; D052899:Pore Forming Cytotoxic Proteins; D013109:Sphingomyelins; C060281:ceramide phosphoethanolamine
Actinoporin; Aegerolysin; Equinatoxin; Lipid domains; Lipid imaging; Lysenin