Super-resolution Microscopy in Plant Cell Imaging.
Although the development of super-resolution microscopy methods dates back to 1994, relevant applications in plant cell imaging only started to emerge in 2010. Since then, the principal super-resolution methods, including structured-illumination microscopy (SIM), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and stimulated emission depletion microscopy (STED), have been implemented in plant cell research. However, progress has been limited due to the challenging properties of plant material. Here we summarize the basic principles of existing super-resolution methods and provide examples of applications in plant science. The limitations imposed by the nature of plant material are reviewed and the potential for future applications in plant cell imaging is highlighted.
Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Faculty of Science, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
George
G
Komis
Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Faculty of Science, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Olga
O
Šamajová
Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Faculty of Science, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Miroslav
M
Ovečka
Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University Olomouc, Faculty of Science, Šlechtitelů 27, 783 71 Olomouc, Czech Republic. Electronic address: jozef.samaj@upol.cz.
Jozef
J
Šamaj
D021621:Imaging, Three-Dimensional; D008853:Microscopy / Q000379:methods*; D059828:Plant Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure
photoactivation localization microscopy; plant cell biology; stimulated emission depletion microscopy; stochastic optical reconstruction microscopy; structured-illumination microscopy; super-resolution microscopy