Antimicrobial Resistance and Molecular Typing of Salmonella Stanley Isolated from Humans, Foods, and Environment.
Salmonella enterica serovar Stanley is an important serovar that has been increasingly identified in human salmonellosis. The present study aimed to investigate the antimicrobial resistance and molecular typing of 88 Salmonella Stanley strains isolated from humans (diarrhea patients, n = 64; and healthy carrier, n = 1), foods (aquatic products, n = 16; vegetable, n = 1; and pork, n = 1), and environment (waste water, n = 2; and river water, n = 3) in Shanghai, China from 2006 to 2012. Nearly half of the strains were resistant to sulfafurazole (43/88, 48.9%), and many were resistant to streptomycin (35/88, 39.8%), tetracycline (22/88, 25%), and nalidixic acid (19/88, 21.6%). Approximately a quarter of the strains (24/88, 27.3%) were resistant to more than three antimicrobials, and five had ACSSuT resistance type. Six clusters (A-F) were identified by pulsed-field gel electrophoresis (PFGE) with 80% similarity. Interestingly, strains in the same cluster identified by PFGE possessed similar antibiotic resistance patterns. PFGE typing also indicated that aquatic products might serve as a transmission reservoir for Salmonella Stanley infections in humans.
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .
Xiaowei
X
Yang
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .|2 Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University , Guangzhou, China .
Dai
D
Kuang
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .|3 Department of Food Science and Nutrition, University of Maryland , College Park, Maryland.
Jianghong
J
Meng
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .
Haijian
H
Pan
4 Microbiology Laboratory, Baoshan District Center for Disease Control and Prevention , Shanghai, China .
Junqing
J
Shen
5 Microbiology Laboratory, Hongkou District Center for Disease Control and Prevention , Shanghai, China .
Jing
J
Zhang
6 Microbiology Laboratory, Shanghai Municipal Ke-Ma-Jia Technology Center for Microbiology , Shanghai, China .
Weimin
W
Shi
7 Shanghai Animal Disease Control Center , Shanghai, China .
Qi
Q
Chen
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .
Xianming
X
Shi
8 Microbiology Laboratory, Shanghai Municipal Center for Disease Control and Prevention , Shanghai, China .
Xuebin
X
Xu
1 Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University , Shanghai, China .|2 Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University , Guangzhou, China .
Jianmin
J
Zhang
D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology; D004197:Disease Reservoirs; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing / Q000379:methods; D045483:Rivers / Q000382:microbiology; D012475:Salmonella / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D012480:Salmonella Infections / Q000382:microbiology; D062065:Wastewater / Q000382:microbiology; D014871:Water Microbiology
D004269:DNA, Bacterial; D062065:Wastewater