Planar monolithic porous polymer layers functionalized with gold nanoparticles as large-area substrates for sensitive surface-enhanced Raman scattering sensing of bacteria.
For the first time, large-area surface-enhanced Raman scattering sensing active substrates using porous polymer monolithic layers have been successfully prepared. Our approach includes a simple photoinitiated polymerization process using glycidyl methacrylate and ethylene dimethacrylate in a glass mold, followed by a chemical reaction of the epoxy functionalities leading to thiols, and the attachment of preformed gold nanoparticles. We demonstrated that this very simple process produced uniform and reproducible large area surfaces that significantly enhance sensitivity of Raman spectroscopy. Experiments were also carried out that confirmed preferential adsorption of living bacteria Escherichia coli from a very dilute solution on the surface of the monolithic layer, and immediate detection of the captured microorganisms using the SERS spectrum.
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Yao
Y
Cao
Department of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Mingyang
M
Lv
Department of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Haijun
H
Xu
International Research Center for Soft Matter, Beijing University of Chemical Technology, Beijing 100029, China.
Frantisek
F
Svec
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Tianwei
T
Tan
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: lvyq@mail.buct.edu.cn.
Yongqin
Y
Lv
D000327:Adsorption; D004926:Escherichia coli / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
D011108:Polymers; D006046:Gold
Bacteria; Gold nanoparticles; Monolithic layer; Porous monolith; Surface-enhanced Raman scattering