Emergence of Magnusiomyces capitatus infections in Western Nepal.
Magnusiomyces capitatus is an emerging opportunistic yeast in the Mediterranean region. We report from Nepal one case of M. capitatus infection and six other cases of colonization/probable infection due to M. capitatus at a tertiary care center. Majority of the patients were immunocompromised, at extreme age, associated with comorbidities, and had history of close contact with livestock and poultry. The isolates were identified by phenotypic and genotypic (ITS and D1/D2 region of 26S rDNA sequence) methods. Molecular typing of the isolates was carried out by amplified fragment length polymorphism. Minimum inhibitory concentration (MIC) of the isolates for amphotericin B, caspofungin, fluconazole, itraconazole, voriconazole, posaconazole, anidulafungin, and micafungin were 2, 0.1-4, 2, 0.12-0.5, 0.12-0.5, 0.25, 1-4, and 1-4 μg/ml, respectively. Presence of M. capitatus infection was not known in Nepal, and the study should alert the clinicians and infectious disease specialists.
Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal supram.gowda@gmail.com.
Hosuru
H
Subramanya Supram
Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal.
Shishir
S
Gokhale
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Arunaloke
A
Chakrabarti
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Shivaprakash M
SM
Rudramurthy
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Sunita
S
Gupta
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Prasanna
P
Honnavar
D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D054458:Amplified Fragment Length Polymorphism Analysis; D000935:Antifungal Agents / Q000494:pharmacology; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D009181:Mycoses / Q000453:epidemiology* / Q000382:microbiology*; D009390:Nepal / Q000453:epidemiology; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers
D000935:Antifungal Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S
Blastoschizomyces capitatus; Magnusiomyces capitatus; emerging fungal infection; immunocompromised