miR-71b regulation of insulin/IGF-1 signaling during starvation in planarians.
Planarians, which have a large population of stem cells called neoblasts, are molecularly tractable model systems used in the study of regeneration. However, planarians have strong resistance to hunger and have developed growth arrest strategies. For example, they can change their size and undergo growth regression during starvation periods. The results of the current study show that the microRNA, miR-71b, and the insulin/IGF-1 signaling pathway have important functions in the development of starvation-induced planarians. We demonstrate tissue-specific expression of miR-71b using in situ hybridization. By employing real-time polymerase chain reaction, we provide evidence that miR-71b is upregulated in starvation-induced planarians. Furthermore, we validate and verify the target genes of miR-71b.
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Wu
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Zhao
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Liu
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Guo
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Liu
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Wang
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Wang
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Li
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.
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Zhang
School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China zhangst@zzu.edu.cn.
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Zhang
D000818:Animals; D007328:Insulin / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D010932:Planarians; D015398:Signal Transduction; D013217:Starvation / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D007328:Insulin; D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I