Extended-spectrum β-lactamase (ESBL) detection directly from urine samples with the rapid isothermal amplification-based eazyplex® SuperBug CRE assay: Proof of concept.
A commercially available assay (eazyplex® SuperBug CRE) detecting the most common carbapenemase and ESBL types was evaluated directly on 50 urine samples. Eazyplex® correctly detected ESBL-encoding genes in all 30 urine samples with confirmed ESBL production (sensitivity 100%). Two specimens showed invalid and one specimen false-positive results (specificity 97.9%).
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland. Electronic address: vladimira.hinic@usb.ch.
V
V
Hinić
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.
J
J
Ziegler
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.
C
C
Straub
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.
D
D
Goldenberger
Division of Clinical Microbiology, University Hospital Basel, Petersgraben 4, 4031, Basel, Switzerland.
R
R
Frei
D001426:Bacterial Proteins / Q000235:genetics / Q000652:urine*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000652:urine*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases / Q000235:genetics / Q000652:urine*
D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; D001618:beta-Lactamases; C063912:carbapenemase
ESBL; Isothermal amplification; Proof of concept; Rapid detection; Urine samples