Effects of low-intensity non-coherent light therapy on the inflammatory process in the calcaneal tendon of ovariectomized rats.
The aim of this experimental study was to investigate the effects of low-intensity light-emitting diode (LED) phototherapy on the inflammatory process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of the inflammatory mediators interleukin (IL)-6, IL-10, and tumor necrosis factor-alpha (TNF-α). Thirty-five female Wistar rats were divided into 4 groups: 3 groups of OVX rats totaling 30 rats (untreated OVX rats [OVX injury group], treated OVX rats [OVX LED group], and control OVX rats; subgroups existed based on the sampling times, which were 3, 7, and 14 days) and 1 group of non-OVX rats (not OVX; n = 5). Tendon injury was induced by trauma using a 208-g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 h after tendonitis with LED therapy and every 48 h thereafter until euthanasia (at 3, 7, or 14 days). The tendons were dissected and stored in liquid nitrogen at -196 °C, thawed only at the time of immunoenzymatic testing (ELISA). Groups treated with LED showed a decrease in the number of pro-inflammatory cells, IL-6, and TNF-α (p <0.05), and an increase in IL-10 (p < 0.05) when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED treatment using the parameters and wavelength of 945 nm in the time periods studied reduced the release of IL-6 and TNF-α and increased the release of IL-10, thereby improving the inflammatory response in OVX rats.
Post-graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brazil.
Carla
C
Helrigle
Post-graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brazil. ptpaulo@terra.com.br.|Post-graduation Program in Rehabilitation Sciences, Universidade Nove de Julho, UNINOVE, Rua Vergueiro, 235, 01504-001, São Paulo, SP, Brazil. ptpaulo@terra.com.br.
Paulo deTarso Camilo
Pd
de Carvalho
Post-graduation Program in Rehabilitation Sciences, Universidade Nove de Julho, UNINOVE, Rua Vergueiro, 235, 01504-001, São Paulo, SP, Brazil.
Heliodora Leão
HL
Casalechi
Post-graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brazil.|Post-graduation Program in Rehabilitation Sciences, Universidade Nove de Julho, UNINOVE, Rua Vergueiro, 235, 01504-001, São Paulo, SP, Brazil.
Ernesto Cesar Pinto
EC
Leal-Junior
Post-graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brazil.
Guilherme Henrique Cardoso
GH
Fernandes
Medical Veterinary Department, Federal University of Goiás-UFG, Jataí, GO, Brazil.
Panmera Almeida
PA
Helrigel
Medical Veterinary Department, Federal University of Goiás-UFG, Jataí, GO, Brazil.
Rogério Leão
RL
Rabelo
Post-graduation Program in Rehabilitation Sciences, Universidade Nove de Julho, UNINOVE, Rua Vergueiro, 235, 01504-001, São Paulo, SP, Brazil.
Ivo
I
de Oliveira Aleixo-Junior
Institute of Science and Technology - ICT, Federal University of São Paulo-Unifesp, São José dos Campos, SP, Brazil.
Flavio
F
Aimbire
Post-graduation Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brazil.|Post-graduation Program in Rehabilitation Sciences, Universidade Nove de Julho, UNINOVE, Rua Vergueiro, 235, 01504-001, São Paulo, SP, Brazil.
Regiane
R
Albertini
D000125:Achilles Tendon / Q000293:injuries / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007249:Inflammation / Q000378:metabolism / Q000628:therapy; D028022:Low-Level Light Therapy; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar
D016207:Cytokines
Calcaneal tendon injury; Inflammation; Low-intensity LED; Ovariectomy; Photobiomodulation therapy