Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia.
Increasing evidence has showed that hypoxia inducible factor-1 (HIF1) has an important role in hypoxia-induced lipid accumulation, a common feature of solid tumors; however, its role remains to be fully elucidated. Adipose differentiation‑related protein (ADRP), a structural protein of lipid droplets, is found to be upregulated under hypoxic conditions. In the present study, an MCF7 breast cancer cell line was used to study the role of ADRP in hypoxia‑induced lipid accumulation. It was demonstrated that hypoxia induced the gene expression of ADRP in a HIF1‑dependent manner. Increases in the mRNA and protein levels of ADRP was accompanied by increased HIF1A activity. In addition, a significant decrease in the mRNA and protein levels of ADRP were detected in presence of siRNA targeting HIF1A. Using a dual‑luciferase reporting experiment and chromatin immunoprecipitation assay, the present study demonstrated that ADRP is a direct target gene of HIF1, and identified a functional hypoxia response element localized 33 bp upstream of the transcriptional start site of the ADRP gene. Furthermore, the present study demonstrated the role of ADRP in low density liporotein (LDL) and very‑LDL uptake‑induced lipid accumulation under hypoxia. The knockdown of ADRP did not reduce HIF1‑induced lipid accumulation under hypoxia. Together, these results showed that ADRP may be not involved in HIF1-induced lipid accumulation.
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Guomin
G
Shen
Department of Gastrointestinal Surgery, International Hospital of Peking University, Beijing 100871, P.R. China.
Ning
N
Ning
Department of Cardiovascular Disease, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010070, P.R. China.
Xingsheng
X
Zhao
Department of Cardiovascular Disease, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010070, P.R. China.
Xi
X
Liu
Department of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 100036, P.R. China.
Guangyu
G
Wang
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Tianzhen
T
Wang
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Ran
R
Zhao
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Chao
C
Yang
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Dongmei
D
Wang
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Pingyuan
P
Gong
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Yan
Y
Shen
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Yongjian
Y
Sun
Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.
Xiao
X
Zhao
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Yinji
Y
Jin
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Weiwei
W
Yang
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Yan
Y
He
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Lei
L
Zhang
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Xiaoming
X
Jin
Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Xiaobo
X
Li
D000818:Animals; D001483:Base Sequence; D015687:Cell Hypoxia; D047369:Chromatin Immunoprecipitation; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000070780:Perilipin-2; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008565:Membrane Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; D034741:RNA, Small Interfering