MiR-564 functions as a tumor suppressor in human lung cancer by targeting ZIC3.
Although miR-564 was reported to be dysregulated in human malignancy, the function and mechanism of miR-564 in tumorigenesis remains unknown. In the present study, we found that miR-564 frequently downregulated in lung cancer cells and significantly inhibited cell proliferation, cell cycle progression, motility, and the tumorigenicity of lung cancer cells. Moreover, we identified zic family member 3 (ZIC3) as a direct target of miR-564. ZIC3 overexpression impaired the suppressive effects of miR-564 on the capacity of lung cancer cells for proliferation and motility. Finally, we detected the expression level of miR-564 and ZIC3 protein in tissue specimens, and found a significant negative correlation between them. Patients with low levels of miR-564 showed a poorer overall survival. Taken together, our present study revealed the tumor suppressor role of miR-564, indicating restoration of miR-564 as a potential therapeutic strategy for the treatment of lung cancer.
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Bin
B
Yang
Department of Nephrology, The Central Hospital of Wuhan, Wuhan, Hubei 430079, China.
Lin
L
Jia
Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Provincial Clinical College of Fujian Medical University, Fuzhou, Fujian 350000, China.
Qiaojuan
Q
Guo
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Hui
H
Ren
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Desheng
D
Hu
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Xiaoyi
X
Zhou
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Qingrong
Q
Ren
Department of Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China. Electronic address: huyp1989@163.com.
Yanping
Y
Hu
Department of Radiation Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China. Electronic address: xietao930@hotmail.com.
Tao
T
Xie
D049109:Cell Proliferation; D005260:Female; D016147:Genes, Tumor Suppressor; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008875:Middle Aged; D011379:Prognosis; D014157:Transcription Factors / Q000378:metabolism*
D018398:Homeodomain Proteins; C577008:MIRN564 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; C491595:ZIC3 protein, human
Invasion; Lung cancer; Proliferation; Tumor suppressor; ZIC3; miR-564