A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage.
Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.
El Nada Fertility and Gynecology Center, 9 Alkobry street, Banha, Qalubeiya, Egypt.
Ehab
E
Darwish
El Nada Fertility and Gynecology Center, 9 Alkobry street, Banha, Qalubeiya, Egypt. Electronic address: Yas.magdi@hotmail.com.
Yasmin
Y
Magdi
D000328:Adult; D061207:Calcium Ionophores / Q000494:pharmacology* / Q000627:therapeutic use; D002970:Cleavage Stage, Ovum / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods; D008297:Male; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D009866:Oogenesis / Q000187:drug effects; D010865:Pilot Projects; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D016896:Treatment Outcome; D055815:Young Adult; D015053:Zygote / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D061207:Calcium Ionophores
2PN arrest; AOA; Ca(2+) ionophore; ICSI; cleavage failure