Distinct phylogeographic structure recognized within Desmazierella acicola.
Desmazierella acicola (anamorph Verticicladium trifidum, Chorioactidaceae) represents a frequent colonizer of pine needles in litter. Considering the global diversity and distribution of pine species, we expected different phylogenetic lineages to exist in different geographical and climatic areas inhabited by these hosts. We compared DNA sequence data with phenotypic characteristics (morphology of the anamorph and growth at three different temperatures) of 43 strains isolated mostly from pine and also spruce needle litter sampled in various geographical areas. Analyses of ITS rDNA recovered eight geographically structured lineages. Fragments of genes for the translation elongation factor 1-α, and the second largest subunit of RNA polymerase II reproduced similar lineages, although not all of them were monophyletic. The similarity in ITS sequences among the clade with samples from Continental-Atlantic Europe and four other clades was lower than 95%. Several lineages exhibit also a tendency toward host specificity to a particular pine species. Growth tests at different temperatures indicated a different tolerance to specific climatic conditions in different geographic areas. However, the surveyed phenotypic characteristics also showed high variation within lineages, most evident in the morphology of the anamorph. Until a morphological study of the teleomorph is carried out, all of these lineages should be treated as distinct populations within a single species.
University of Ljubljana, Biotechnical faculty, Jamnikarjeva 101, 1000 Ljubljana, Slovenia, and Charles University in Prague, Faculty, of Science, Department of Botany, Benátská 2, CZ-12801, Prague, Czech Republic.
Tijana
T
Martinović
Charles University in Prague, Faculty of Science, Department of Botany, Benátská 2, CZ-12801, Prague, Czech Republic ondrej.koukol@natur.cuni.cz.
Ondřej
O
Koukol
School of Pharmacy, Nihon University, Funabashi, Chiba 274-8555, Japan.
Dai
D
Hirose
D001203:Ascomycota / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D039642:Eukaryotic Initiation Factors / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005828:Genetics, Population; D008969:Molecular Sequence Data; D010802:Phylogeny; D058974:Phylogeography; D028222:Picea / Q000382:microbiology*; D028223:Pinus / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D039642:Eukaryotic Initiation Factors; D005656:Fungal Proteins
Pezizales; anamorph morphology; multigene analysis; population structure