A fluorometric microarray with ZnO substrate-enhanced fluorescence and suppressed "coffee-ring" effects for fluorescence immunoassays.
A glass slide was first patterned with hydrophobic hexadecyltrimethoxysilane (HDS) and then microspotted with hydrophilic ZnO nanoparticles in an aminopropyltriethoxysilane (APS) matrix. The resulting HDS-ZnO-APS microarray could present the capability of suppressing the undesirable "coffee-ring" effects through its hydrophobic pattern so as to allow the fabrication of ZnO-APS testing microspots with a highly dense and uniform distribution. The lotus-like "self-cleaning" function could also be expected to effectively curb the cross contamination of multiple sample droplets. More importantly, the introduction of ZnO nanoparticles could endow the testing microspots with substrate-enhanced fluorescence leading to signal-amplification microarray fluorometry. The practical application of the developed HDS-ZnO-APS microarray was investigated by the sandwiched fluorometric immunoassays of human IgG, showing a linear detection range from 0.010 to 10.0 ng mL(-1). Such a throughput-improved fluorometric microarray could be tailored for probing multiple biomarkers in complicated media like serum or blood.
Shandong Province Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu City, Shandong Province 273165, P. R. China. huawangqfnu@126.com.
Shuying
S
Li
Minmin
M
Dong
Rui
R
Li
Liyan
L
Zhang
Yuchun
Y
Qiao
Yao
Y
Jiang
Wei
W
Qi
Hua
H
Wang
D015590:Fluorophotometry / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D011437:Propylamines / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D012821:Silanes / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry*
D007074:Immunoglobulin G; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D015034:Zinc Oxide