Inducible gene expression system by 3-hydroxypropionic acid.
BACKGROUND
3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and excretion.


RESULTS
Here we report the presence of unique inducible gene expression systems in Pseudomonas denitrificans and other microorganisms. In P. denitrificans, transcription of three genes (hpdH, mmsA and hbdH-4) involved in 3-HP degradation was upregulated by 3-HP by the action of a transcriptional regulator protein, LysR, and a cis-acting regulatory site for LysR binding. Similar inducible systems having an LysR transcriptional regulator were identified in other microorganisms that also could degrade 3-HP. A docking study showed that the 3-HP binding pocket is located between the N-terminal helix-turn-helix motif and the C-terminal cofactor-binding domain.


CONCLUSIONS
This LysR-regulated 3-HP-inducible system should prove useful for control of the level of gene expression in response to 3-HP.
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.|Department of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou, 213164 China.
Shengfang
S
Zhou
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.
Satish Kumar
SK
Ainala
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.
Eunhee
E
Seol
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.
Trinh Thi
TT
Nguyen
School of Chemical and Biomolecular Engineering, Pusan National University, San 30 Jangeon-dong, Geumjeong-gu, Busan, 609-735 Republic of Korea.
Sunghoon
S
Park
3-Hydroxypropionic acid; Inducible; LysR; hbdH; hpdH; mmsA