A rapid qPCR method to investigate the circulation of the yeast Wickerhamomyces anomalus in humans.
The yeast Wickerhamomyces anomalus has been proposed for many biotechnological applications in the food industry. However, a number of opportunistic pathogenic strains have been reported as causative agents of nosocomial fungemia. Recognition of potentially pathogenic isolates is an important challenge for the future commercialization of this yeast. The isolation of W. anomalus from different matrices and, recently, from mosquitoes, requires further investigations into its circulation in humans. Here we present a qPCR protocol for the detection of W. anomalus in human blood samples and the results of a screening of 525 donors, including different classes of patients and healthy people.
Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
Sara
S
Epis
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Aida
A
Capone
Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
Elena
E
Martin
Unit of Microbiology, St. Orsola-Malpighi, University Hospital, Bologna, Italy.
Michela
M
Paolucci
Department of Veterinary Sciences and Public Health, University of Milan, Milan, Italy.
Chiara
C
Bazzocchi
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Matteo
M
Valzano
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Jovana
J
Bozic
Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.
Stefano
S
Novati
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Guido
G
Favia
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Irene
I
Ricci
D001769:Blood / Q000382:microbiology*; D006801:Humans; D009181:Mycoses / Q000097:blood / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
Wickerhamomyces anomalus; fungemia; mosquito vectors; opportunistic pathogen; qPCR