Corticomedullary difference in the effects of dietary Ca²⁺ on tight junction properties in thick ascending limbs of Henle's loop.
The thick ascending limb of Henle's loop (TAL) drives an important part of the reabsorption of divalent cations. This reabsorption occurs via the paracellular pathway formed by the tight junction (TJ), which in the TAL shows cation selectivity. Claudins, a family of TJ proteins, determine the permeability and selectivity of this pathway. Mice were fed with normal or high-Ca(2+) diet, and effects on the reabsorptive properties of cortical and medullary TAL segments were analysed by tubule microdissection and microperfusion. Claudin expression was investigated by immunostaining and quantitative PCR. We show that the TAL adapted to high Ca(2+) load in a sub-segment-specific manner. In medullary TAL, transcellular NaCl transport was attenuated. The transepithelial voltage decreased from 10.9 ± 0.6 mV at control diet to 8.3 ± 0.5 mV at high Ca(2+) load, thereby reducing the driving force for Ca(2+) and Mg(2+) uptake. Cortical TAL showed a reduction in paracellular Ca(2+) and Mg(2+) permeabilities from 8.2 ± 0.7 to 6.2 ± 0.5 ∙ 10(-4) cm/s and from 4.8 ± 0.5 to 3.0 ± 0.2 · 10(-4) cm/s at control and high-Ca(2+) diet, respectively. Expression, localisation and regulation of claudins 10, 14, 16 and 19 differed along the corticomedullary axis: Towards the cortex, the main site of divalent cation reabsorption in TAL, high-Ca(2+) intake led to a strong upregulation of claudin-14 within TAL TJs while claudin-16 and -19 were unaltered. Towards the inner medulla, only claudin-10 was present in TAL TJ strands. In summary, high-Ca(2+) diet induced a reduction of divalent cation reabsorption via a diminution of NaCl transport and driving force in mTAL and via decreased paracellular permeabilities in cTAL. We reveal an important regulatory pattern along the corticomedullary axis and improve the understanding how the kidney disposes of detrimental excess Ca(2+).
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany.
Allein
A
Plain
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany.
Vera C
VC
Wulfmeyer
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany.
Susanne
S
Milatz
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany.
Adrian
A
Klietz
Washington University Renal Division, St. Louis, MO, USA.
Jianghui
J
Hou
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany.
Markus
M
Bleich
Institute of Physiology, Christian-Albrechts-University Kiel, Olshausenstraße 40, Kiel, 24098, Germany. n.himmerkus@physiologie.uni-kiel.de.
Nina
N
Himmerkus
D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002136:Calcium, Dietary / Q000494:pharmacology*; D057167:Claudins / Q000235:genetics / Q000378:metabolism; D005260:Female; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism; D008138:Loop of Henle / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D065608:Renal Reabsorption; D012965:Sodium Chloride / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
D002136:Calcium, Dietary; D057167:Claudins; D012965:Sodium Chloride; D008274:Magnesium; D002118:Calcium
Calcium; Claudin; Ion transport; Microperfusion; Nephron; Paracellular permeability