Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors.
Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4(+)T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia.
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Mei
M
Wu
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Hongfeng
H
Ge
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Shue
S
Li
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Hailiang
H
Chu
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Shili
S
Yang
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Xiaoxing
X
Sun
Department of Hematology, The People's Hospital of Bozhou, Bozhou 236800, China.
Zhenxia
Z
Zhou
Department of Hematology, First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, China.
Xiongpeng
X
Zhu
D000954:Antigens, Surface / Q000378:metabolism; D002454:Cell Differentiation; D015232:Dinoprostone / Q000494:pharmacology; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D016130:Immunophenotyping; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000276:immunology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D000097043:Interleukin-22
D000954:Antigens, Surface; D015232:Dinoprostone; D007378:Interleukins; D000097043:Interleukin-22