Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study.
Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging. However, it tends to be taken overdose or long term in these years, which has resulted in liver damage reported in many countries. In this study, the indicative roles of nine bile acids (BAs) were evaluated to offer potential biomarkers for HSW induced liver injury. Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment. Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized. Significant change was observed for the concentrations of nine BAs in treatment groups compared with normal control; In the PLS-DA plots of nine BAs in bile, normal control and raw HSW groups were separately clustered and could be clearly distinguished, GDCA was selected as the distinguished components for raw HSW overdose treatment group. In the PLS-DA plots of nine BAs in serum, the normal control and raw HSW overdose treatment group were separately clustered and could be clearly distinguished, and HDCA was selected as the distinguished components for raw HSW overdose treatment group. The results indicated the perturbation of nine BAs was associated with HSW induced liver injury; GDCA in bile, as well as HDCA in serum could be selected as potential biomarkers for HSW induced liver injury; it also laid the foundation for the further search on the mechanisms of liver injury induced by HSW.
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Qin
Q
Dong
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Na
N
Li
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Qi
Q
Li
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Cong-En
CE
Zhang
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Wu-Wen
WW
Feng
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Guang-Quan
GQ
Li
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Rui-Yu
RY
Li
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Can
C
Tu
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.
Xue
X
Han
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Zhao-Fang
ZF
Bai
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Ya-Ming
YM
Zhang
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Ming
M
Niu
Beijing Friendship Hospital, Capital Medical University Beijing, China.
Zhi-Jie
ZJ
Ma
Integrative Medicine Center, 302 Military Hospital Beijing, China.
Xiao-He
XH
Xiao
China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China.
Jia-Bo
JB
Wang
LC-MS; Polygonum multiflorum; bile acid; biomarker; liver injury