A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans.
We report here the protective effects of a methanol extract from a cultivated strain of the red seaweed, Chondrus crispus, against β-amyloid-induced toxicity, in a transgenic Caenorhabditis elegans, expressing human Aβ1-42 gene. The methanol extract of C. crispus (CCE), delayed β-amyloid-induced paralysis, whereas the water extract (CCW) was not effective. The CCE treatment did not affect the transcript abundance of amy1; however, Western blot analysis revealed a significant decrease of Aβ species, as compared to untreated worms. The transcript abundance of stress response genes; sod3, hsp16.2 and skn1 increased in CCE-treated worms. Bioassay guided fractionation of the CCE yielded a fraction enriched in monogalactosyl diacylglycerols (MGDG) that significantly delayed the onset of β-amyloid-induced paralysis. Taken together, these results suggested that the cultivated strain of C. crispus, whilst providing dietary nutritional value, may also have significant protective effects against β-amyloid-induced toxicity in C. elegans, partly through reduced β-amyloid species, up-regulation of stress induced genes and reduced accumulation of reactive oxygen species (ROS).
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. jatinder.sangha@dal.ca.
Jatinder Singh
JS
Sangha
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. owenwally@gmail.com.
Owen
O
Wally
Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. arjun.banskota@nrc-cnrc.gc.ca.
Arjun H
AH
Banskota
Aquatic and Crop Resources Development, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada. Roumiana.Stefanova@nrc.gc.ca.
Roumiana
R
Stefanova
Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. jhafting@acadian.ca.
Jeff T
JT
Hafting
Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada. Alan.Critchley@acadian.ca.
Alan T
AT
Critchley
Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. bprithiviraj@dal.ca.
Balakrishnan
B
Prithiviraj
D016229:Amyloid beta-Peptides / Q000633:toxicity; D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D017173:Caenorhabditis elegans / Q000187:drug effects*; D044723:Chondrus / Q000737:chemistry*; D006801:Humans; D000432:Methanol / Q000737:chemistry; D010243:Paralysis / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects
D016229:Amyloid beta-Peptides; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000432:Methanol
Caenorhabditis elegans; cultivated Chondrus crispus; glycolipid; monogalactosyl diacylglycerol (MGDG); neuroprotection; red seaweeds; β-amyloid