Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua.
Mesenchymal stem cells (MSCs) are one of the most attractive cell types for cell-based bone tissue repair applications. Fetal-derived MSCs and maternal-derived MSCs have been isolated from chorionic villi of human term placenta and the decidua basalis attached to the placenta following delivery, respectively. Chorionic-derived MSCs (CMSCs) and decidua-derived MSCs (DMSCs) generated in this study met the MSCs criteria set by International Society of Cellular Therapy. These criteria include: (i) adherence to plastic; (ii) >90% expression of CD73, CD105, CD90, CD146, CD44 and CD166 combined with <5% expression of CD45, CD19 and HLA-DR; and (iii) ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. In vivo subcutaneous implantation into SCID mice showed that both bromo-deoxyuridine (BrdU)-labelled CMSCs and DMSCs when implanted together with hydroxyapatite/tricalcium phosphate particles were capable of forming ectopic bone at 8-weeks post-transplantation. Histological assessment showed expression of bone markers, osteopontin (OPN), osteocalcin (OCN), biglycan (BGN), bone sialoprotein (BSP), and also a marker of vasculature, alpha-smooth muscle actin (α-SMA). This study provides evidence to support CMSCs and DMSCs as cellular candidates with potent bone forming capacity.
Department of Obstetrics and Gynaecology, Royal Women's Hospital, The University of Melbourne, Parkville, Victoria, Australia; Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia.
Gina D
GD
Kusuma
Mesenchymal Stem Cell Laboratory, Faculty of Health Sciences, School of Medical Sciences, University of Adelaide, Adelaide, Australia; Colgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, Australia.
Danijela
D
Menicanin
Mesenchymal Stem Cell Laboratory, Faculty of Health Sciences, School of Medical Sciences, University of Adelaide, Adelaide, Australia.
Stan
S
Gronthos
Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia; Centre for Genetic Diseases, Monash Institute of Medical Research-Prince Henry's Institute of Medical Research and Monash University, Clayton, Victoria, Australia.
Ursula
U
Manuelpillai
King Abdullah International Medical Research Center/ King Saud Bin Abdulaziz University for Health Sciences, College of Science and Health Professions, King Abdulaziz Medical City-National Guard Health Affairs, Riyadh, Kingdom of Saudi Arabia.
Mohamed H
MH
Abumaree
Victorian Clinical Genetics Services (VCGS), Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Parkville, Victoria, Australia.
Mark D
MD
Pertile
Department of Obstetrics and Gynaecology, Royal Women's Hospital, The University of Melbourne, Parkville, Victoria, Australia; Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia.
Shaun P
SP
Brennecke
Department of Obstetrics and Gynaecology, Royal Women's Hospital, The University of Melbourne, Parkville, Victoria, Australia; Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia.
Bill
B
Kalionis
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology* / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D016513:Mice, SCID; D010012:Osteogenesis / Q000502:physiology*; D010920:Placenta / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy
D015415:Biomarkers