Buyang Huanwu decoction up-regulates Notch1 gene expression in injured spinal cord.
Expression of genes in the Notch signaling pathway is altered in the injured spinal cord, which indicates that Notch participates in repair after spinal cord injury. Buyang Huanwu decoction, a traditional Chinese herbal preparation, can promote the growth of nerve cells and nerve fibers; however, it is unclear whether Buyang Huanwu decoction affects the Notch signaling pathway in injured spinal cord. In this study, a rat model was established by injuring the T10 spinal cord. At 2 days after injury, rats were intragastrically administered 2 mL of 0.8 g/mL Buyang Huanwu decoction daily until sacrifice. Real-time reverse transcription polymerase chain reaction analysis demonstrated that at 7, 14 and 28 days after injury, the expression of Notch1 was increased in the Buyang Huanwu decoction group compared with controls. These findings confirm that Buyang Huanwu decoction can promote the expression of Notch1 in rats with incomplete spinal cord injury, and may indicate a mechanism to promote the repair of spinal cord injury.
Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
Zhan-Peng
ZP
Guo
College of Nursing, Liaoning Medical University, Jinzhou, Liaoning Province, China.
Mi-Na
MN
Huang
College of Basic Medical Sciences, Liaoning Medical University, Jinzhou, Liaoning Province, China.
An-Qi
AQ
Liu
Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
Ya-Jiang
YJ
Yuan
Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
Jian-Bo
JB
Zhao
Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
Xi-Fan
0000-0003-2661-8629
XF
Mei
Buyang Huanwu decoction; Chinese medicine; Notch1 signaling pathway; nerve regeneration; neural regeneration; spinal cord injury