Flow Cytometric Analysis of Human Small Intestinal Lymphoid Cells.
Flow cytometry is a powerful technique allowing simultaneous analysis of numerous morphological and phenotypic characteristics of cells and cellular constituents. Improvements in cell isolation techniques in recent years have enabled flow cytometric analyses of cells derived from tissue biopsies. Here we describe a method for isolating and analyzing small intestinal lymphoid cells using flow cytometry.
Department of Surgery, Trinity Centre for Health Sciences, St James's Hospital, James Street, Dublin 8, Ireland. margaret.a.dunne@gmail.com.|National Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin 12, Ireland. margaret.a.dunne@gmail.com.|Department of Immunology, Institute of Molecular Medicine, St James's Hospital, Dublin 8, Ireland. margaret.a.dunne@gmail.com.
Margaret R
MR
Dunne
D001706:Biopsy; D002469:Cell Separation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007421:Intestine, Small / Q000166:cytology* / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology*
Antibodies; Epithelium; Flow cytometry; Intestinal biopsy; Intraepithelial lymphocytes; Lamina propria; Small intestine; Tissue dissociation