Investigation of Bovine Serum Albumin (BSA) Attachment onto Self-Assembled Monolayers (SAMs) Using Combinatorial Quartz Crystal Microbalance with Dissipation (QCM-D) and Spectroscopic Ellipsometry (SE).
Understanding protein adsorption kinetics to surfaces is of importance for various environmental and biomedical applications. Adsorption of bovine serum albumin to various self-assembled monolayer surfaces including neutral and charged hydrophilic and hydrophobic surfaces was investigated using in-situ combinatorial quartz crystal microbalance with dissipation and spectroscopic ellipsometry. Adsorption of bovine serum albumin varied as a function of surface properties, bovine serum albumin concentration and pH value. Charged surfaces exhibited a greater quantity of bovine serum albumin adsorption, a larger bovine serum albumin layer thickness, and increased density of bovine serum albumin protein compared to neutral surfaces at neutral pH value. The quantity of adsorbed bovine serum albumin protein increased with increasing bovine serum albumin concentration. After equilibrium sorption was reached at pH 7.0, desorption of bovine serum albumin occurred when pH was lowered to 2.0, which is below the isoelectric point of bovine serum albumin. Our data provide further evidence that combinatorial quartz crystal microbalance with dissipation and spectroscopic ellipsometry is a sensitive analytical tool to evaluate attachment and detachment of adsorbed proteins in systems with environmental implications.
Department of Civil Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
Hanh T M
HT
Phan
Department of Civil Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
Shannon
S
Bartelt-Hunt
Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
Keith B
KB
Rodenhausen
Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
Mathias
M
Schubert
Department of Medical Microbiology and Immunology, Creighton University, Omaha, Nebraska, United States of America.
Jason C
JC
Bartz
D000327:Adsorption; D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D019906:Nuclear Magnetic Resonance, Biomolecular; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D012710:Serum Albumin, Bovine