Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway.
Inflammation and oxidative stress have been reported to play critical roles in the pathogenesis of neurodegenerative disease. Forsythiaside A, a phenylethanoside product isolated from air-dried fruits of Forsythia suspensa, has been reported to have anti-inflammatory and antioxidant effects. In this study, the anti-inflammatory effects of forsythiaside A on LPS-stimulated BV2 microglia cells and primary microglia cells were investigated. The production of inflammatory mediators TNF-α, IL-1β, NO and PGE2 were detected in this study. NF-κB, nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) expression were detected by western blot analysis. Our results showed that forsythiaside A significantly inhibited LPS-induced inflammatory mediators TNF-α, IL-1β, NO and PGE2 production. LPS-induced NF-κB activation was suppressed by forsythiaside A. Furthermore, forsythiaside A was found to up-regulate the expression of Nrf2 and HO-1. In conclusion, this study demonstrates that forsythiaside A inhibits LPS-induced inflammatory responses in BV2 microglia cells and primary microglia cells through inhibition of NF-κB activation and activation of Nrf2/HO-1 signaling pathway.
The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.|Department of Neurosurgery, No. 208 Hospital of PLA, Changchun, China.
Yue
Y
Wang
Department of Encephalopathy, The Affiliated Hospital of Changchun Chinese Medicine University, Changchun, China.
Hongfei
H
Zhao
The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.
Chuangxin
C
Lin
Nursing Department, No. 461 Hospital of PLA, Changchun, China.
Jie
J
Ren
The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China. zhangshizhongwb36@163.com.
Shizhong
S
Zhang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D006027:Glycosides / Q000494:pharmacology*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D052003:NF-kappa B p52 Subunit / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D006027:Glycosides; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; D052003:NF-kappa B p52 Subunit; C495636:Nfe2l2 protein, mouse; D009569:Nitric Oxide; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; C058049:forsythiaside
Forsythiaside A; Inflammatory mediators; LPS; NF-κB; Nrf2