High 15-F2t-Isoprostane Levels in Patients with a Previous History of Nonmelanoma Skin Cancer: The Effects of Supplementary Antioxidant Therapy.
BACKGROUND
Phase I of this study was aimed at comparing the profiles of oxidative stress biomarkers in patients with history of nonmelanoma skin cancer (NMSC), previously treated with surgery, to the healthy subjects. Phase II aimed to evaluate the effects of supplementary antioxidant therapy on the levels of biomarkers in the case group.


METHODS
In Phase I, oxidative stress biomarkers were measured in blood samples obtained from 24 healthy subjects and 60 patients with history of NMSC previously treated with surgery. In Phase II, the 60 patients with history of NMSC were randomized into two subgroups, one receiving placebo (n = 34) and the other (n = 26) receiving vitamin C, vitamin E, and zinc supplementation for 8 weeks, followed by reevaluation of biomarkers.


RESULTS
In Phase I, patients with history of NMSC showed increased plasma concentrations of all biomarkers, but only 15-F2t-isoprostane was significantly higher than in the healthy subjects. Risk of NMSC increased by 4% for each additional 1 pg/mL increase in 15-F2t-isoprostane. In Phase II, supplementation did not significantly reduce levels of oxidative stress biomarkers.


CONCLUSIONS
Patients with history of NMSC had significantly high 15-F2t-isoprostane plasma levels; supplementation did not result in significant reduction of oxidative stress biomarkers. This trial was registered with ClinicalTrials.gov (ID NCT02248584).
Department of Nutrition, Federal University of Piauí (UFPI), Campus Universitário Ministro Petrônio Portella, 64049-550 Teresina, PI, Brazil ; School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil.
Betânia de Jesus E Silva de Almendra
Bde J
Freitas
School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil.
Gustavo Rafaini
GR
Lloret
School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil.
Marília Berlofa
MB
Visacri
School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil.
Bruna Taliani
BT
Tuan
Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), 13083-859 Campinas, SP, Brazil.
Lais Sampaio
LS
Amaral
Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), 13083-859 Campinas, SP, Brazil.
Daniele
D
Baldini
Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), 13083-859 Campinas, SP, Brazil.
Vanessa Marcílio
VM
de Sousa
Department of Nutrition, Federal University of Piauí (UFPI), Campus Universitário Ministro Petrônio Portella, 64049-550 Teresina, PI, Brazil.
Laís Lima
LL
de Castro
Department of Nutrition, Federal University of Piauí (UFPI), Campus Universitário Ministro Petrônio Portella, 64049-550 Teresina, PI, Brazil.
Jordana Rayane Sousa
JR
Aguiar
Department of Biological and Health Science Center, Mackenzie Presbyterian University, 01302-907 São Paulo, SP, Brazil.
Eder de Carvalho
Ede C
Pincinato
School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil ; Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), 13083-859 Campinas, SP, Brazil.
Priscila Gava
PG
Mazzola
School of Medical Sciences (FCM), University of Campinas (UNICAMP), 13083-887 Campinas, SP, Brazil ; Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), 13083-859 Campinas, SP, Brazil.
Patricia
P
Moriel
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage; D001205:Ascorbic Acid / Q000008:administration & dosage; D014408:Biomarkers, Tumor / Q000097:blood*; D019587:Dietary Supplements; D015237:Dinoprost / Q000031:analogs & derivatives; D005260:Female; D006801:Humans; D028421:Isoprostanes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D012878:Skin Neoplasms / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D014810:Vitamin E / Q000008:administration & dosage; D015032:Zinc / Q000008:administration & dosage
D000975:Antioxidants; D014408:Biomarkers, Tumor; D028421:Isoprostanes; D014810:Vitamin E; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D015032:Zinc; D001205:Ascorbic Acid