On-Line Restricted Access Molecularly Imprinted Solid-Phase Extraction of Selective Serotonin Reuptake Inhibitors Directly from Untreated Human Plasma Samples Followed by HPLC-UV Analysis.
In this article, we proposed a new restricted access molecularly imprinted polymer coated with bovine serum albumin (RAMIP-BSA) to be used in a multidimensional chromatographic system applied for the analysis of six selective serotonin reuptake inhibitors (SSRIs) directly from untreated human plasma samples. Fluoxetine, methacrylic acid and ethylene glycol dimethacrylate were used as the template, functional monomer and cross-linker, respectively. The imprinted polymer was covered with a bovine serum albumin (BSA) layer via the interconnections between the amine groups of the BSA using glutaraldehyde as a cross-linker. The obtained RAMIP-BSA was able to extract the SSRIs directly from the human plasma, while ∼100% of the proteins were excluded from the sample. Selectivity coefficients were calculated for fluoxetine (template) in comparison with venlafaxine, duloxetine, citalopram, fluvoxamine, paroxetine and sertraline, and the values were >1 in all cases, attesting to the presence of binding sites in the imprinted polymer. The method presented analytical ranges from 20 to 500 µg/L and correlation coefficients >0.99 for all of the SSRIs (fluoxetine, venlafaxine, duloxetine, citalopram, fluvoxamine and sertraline). Precision and accuracy presented variation coefficients and relative errors <14.5% and within the range of -19.18 to 3.8%, respectively. In all cases, the apparent recoveries were >85%. The proposed method was able to analyze three samples per hour, and each column was used at least 50 times without any significant changes in its performance.
Laboratory of Toxicant and Drug Analysis-LATF, Federal University of Alfenas-Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000 Alfenas, MG, Brazil.
Katrine Kyona Muniz Sirgom
KK
da Silva
Laboratory of Toxicant and Drug Analysis-LATF, Federal University of Alfenas-Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000 Alfenas, MG, Brazil.
Vanessa Bergamin
VB
Boralli
Institute of Exact Sciences, Federal University of Alfenas - Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000 Alfenas, MG, Brazil.
Célio
C
Wisniewski
Laboratory of Toxicant and Drug Analysis-LATF, Federal University of Alfenas-Unifal-MG, Gabriel Monteiro da Silva St. 700, 37130-000 Alfenas, MG, Brazil eduardocfig@yahoo.com.br.
Eduardo Costa
EC
Figueiredo
D002851:Chromatography, High Pressure Liquid; D000068598:Data Accuracy; D005473:Fluoxetine / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D017367:Selective Serotonin Reuptake Inhibitors / Q000097:blood / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
D005473:Fluoxetine; D005976:Glutaral; D008689:Methacrylates; D011108:Polymers; D017367:Selective Serotonin Reuptake Inhibitors; D012710:Serum Albumin, Bovine; C008384:methacrylic acid; C004919:ethylene dimethacrylate