SENP1 inhibits the IH-induced apoptosis and nitric oxide production in BV2 microglial cells.
To reveal SUMOylation and the roles of Sentrin-specific proteases (SENP)s in microglial cells under Intermittent hypoxia (IH) condition would provide more intensive view of understanding the mechanisms of IH-induced central nervous system (CNS) damage. Hence, in the present study, we detected the expression levels of SENPs in microglial cells under IH and normoxia conditions via RT-PCR assay. We found that SENP1 was significantly down-regulated in cells exposure to IH. Subsequently, the effect of IH for the activation of microglia and the potential roles of SENP1 in the SENP1-overexpressing cell lines were investigated via Western blotting, RT-PCR and Griess assay. The present study demonstrated the apoptosis-inducing and activating role of IH on microglia. In addition, we revealed that the effect of IH on BV-2 including apoptosis, nitric oxide synthase (iNOS) expression and nitric oxide (NO) induction can be attenuated by SENP1 overexpression. The results of the present study are of both theoretical and therapeutic significance to explore the potential roles of SENP1 under IH condition and elucidated the mechanisms underlying microglial survival and activation.
Department of Respiratory Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China. Electronic address: liusongyy@hotmail.com.
Song
S
Liu
Department of Medical Oncology, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Zhong-hua
ZH
Wang
Department of Respiratory Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Bo
B
Xu
Department of Neurology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
Kui
K
Chen
Department of Respiratory Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Jin-yuan
JY
Sun
Department of Respiratory Medicine, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Lian-ping
LP
Ren
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053181:Caspase 8 / Q000378:metabolism; D002460:Cell Line; D003546:Cysteine Endopeptidases; D015536:Down-Regulation; D010450:Endopeptidases / Q000502:physiology*; D000860:Hypoxia / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*
D009569:Nitric Oxide; D010450:Endopeptidases; D053181:Caspase 8; D003546:Cysteine Endopeptidases; C501974:Senp1 protein, mouse
CNS damage; Intermittent hypoxia; Microglia; SENP1