Design, synthesis, and characterization of nucleosomes containing site-specific DNA damage.
How DNA damaged is formed, recognized, and repaired in chromatin is an area of intense study. To better understand the structure activity relationships of damaged chromatin, mono and dinucleosomes containing site-specific damage have been prepared and studied. This review will focus on the design, synthesis, and characterization of model systems of damaged chromatin for structural, physical, and enzymatic studies.
Department of Chemistry, Washington University, St. Louis,MO 63130, USA. Electronic address: taylor@wustl.edu.
John-Stephen
JS
Taylor
D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D056890:Eukaryota / Q000378:metabolism; D006801:Humans; D009707:Nucleosomes; D013329:Structure-Activity Relationship
D009707:Nucleosomes; D004247:DNA
AP sites; Chromatin; DNA damage; DNA photoproducts; DNA repair; Dinucleosome; Histones; Linker; Nucleosome; Photocaged; Solid phase DNA synthesis