Biofilm production in Staphylococcus epidermidis strains, isolated from the skin of hospitalized patients: genetic and phenotypic characteristics.
A major virulence factor of Staphylococcus epidermidis is its ability to form biofilms, permitting it to adhere to a surface and, in turn, to form a mucoid layer on polymer surfaces. Multiple factors have been found to influence bacterial attachment. Currently, this bacterium is commonly associated with hospital infections as a consequence of its ability to colonize, albeit accidentally, medical devices. This study investigated the genetic and phenotypic formation of biofilm in 105 S. epidermidis strains isolated from the skin of hospitalized patients. Fifty-eight of these patients were positive for the mecA gene (MRSE) and 47 were found to be negative (MSSE). Genetic characterizations were performed for the detection of the mecA, icaADBC, atlE, aap, bhp, IS256 and agr groups by PCR. Biofilm production was examined by culturing the strains in TBS medium and TBS with 0.5 and 1% respectively of glucose, and a semiquantitative assay on tissue culture plates was used. Although a molecular analysis estimate of detailed biofilm formation is costly in terms of time and complexity, a semiquantitative assay can be proposed as a rapid and cheap diagnostic method for initial screening to discover virulent strains. We confirmed a close correlation between genetic and phenotypic characteristics, highlighting the fact that, when S. epidermidis isolates were cultured in TSB with 1% of glucose, an increase in biofilm production was observed, as confirmed by positivity for the ica locus by molecular analysis.
"G. D'Alessandro" Department of Sciences for Health Promotion and Mother-Child Care - University of Palermo.
Cinzia
C
Calà
"G. D'Alessandro" Department of Sciences for Health Promotion and Mother-Child Care - University of Palermo.
Emanuele
E
Amodio
"G. D'Alessandro" Department of Sciences for Health Promotion and Mother-Child Care - University of Palermo.
Enza
E
Di Carlo
Department of Sciences for Health Promotion and Mother-Child Care "G. D'Alessandro" - University of Palermo.
Roberta
R
Virruso
"G. D'Alessandro" Department of Sciences for Health Promotion and Mother-Child Care - University of Palermo.
Teresa
T
Fasciana
"G. D'Alessandro" Department of Sciences for Health Promotion and Mother-Child Care - University of Palermo.
Anna
A
Giammanco
D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D003428:Cross Infection / Q000382:microbiology*; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D010641:Phenotype; D012867:Skin / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
D001426:Bacterial Proteins; C487495:MecA protein, Staphylococcus epidermidis
Biofilm; Methicillin resistance; S. epidermidis; Skin