Oxidative Stress Parameters in Saliva and Its Association with Periodontal Disease and Types of Bacteria.
OBJECTIVE
To determine the association between oxidative stress parameters with periodontal disease, bleeding, and the presence of different periodontal bacteria.


METHODS
A cross-sectional study in a sample of eighty-six patients, divided into three groups depending on their periodontal status. Thirty-three with chronic periodontitis, sixteen with gingivitis, and thirty-seven with periodontal healthy as control. Oxidative stress biomarkers (8-OHdG and MDA), total antioxidant capacity (TAOC), and the activity of two antioxidant enzymes (GPx and SOD) were determined in saliva. Subgingival plaque samples were obtained from the deepest periodontal pocket and PCR was used to determine the presence of the 6 fimA genotypes of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Treponema denticola.


RESULTS
Periodontal disease was found to be associated with increased oxidative stress parameter levels. These levels rose according to the number and type of different periodontal bacteria found in the periodontal pockets. The presence of different types of periodontal bacteria is predictive independent variables in linear regresion models of oxidative stress parameters as dependent variable, above all 8-OHdG.


CONCLUSIONS
Oxidative stress parameter levels are correlated with the presence of different types of bacteria. Determination of these levels and periodontal bacteria could be a potent tool for controlling periodontal disease development.
Department d'Estomatología, Facultat de Medicina y Odontología, Universitat de València, C/ Gascó Oliag 1, 46010 Valencia, Spain.
Jose Manuel
JM
Almerich-Silla
Department d'Estomatología, Facultat de Medicina y Odontología, Universitat de València, C/ Gascó Oliag 1, 46010 Valencia, Spain.
Jose María
JM
Montiel-Company
Fundación Investigación Hospital Clínico Universitario de Valencia, Instituto de Investigación Sanitaria INCLIVA, C/ Menéndez y Pelayo 4, 46010 Valencia, Spain.
Sara
S
Pastor
Fundación Investigación Hospital Clínico Universitario de Valencia, Instituto de Investigación Sanitaria INCLIVA, C/ Menéndez y Pelayo 4, 46010 Valencia, Spain.
Felipe
F
Serrano
Department d'Estomatología, Facultat de Medicina y Odontología, Universitat de València, C/ Gascó Oliag 1, 46010 Valencia, Spain.
Miriam
M
Puig-Silla
Fundación Investigación Hospital Clínico Universitario de Valencia, Instituto de Investigación Sanitaria INCLIVA, C/ Menéndez y Pelayo 4, 46010 Valencia, Spain.
Francisco
F
Dasí
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D064307:Microbiota; D018384:Oxidative Stress; D010518:Periodontitis / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D012463:Saliva / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D015415:Biomarkers; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine