Histological Effects of Enamel Matrix Derivative on Exposed Dental Pulp.
BACKGROUND
Direct pulp capping procedure is a therapeutic application of a drug on exposed tooth pulp in order to ensure the closure of the pulp chamber and to allow the healing process to take place.


OBJECTIVE
The aim of this study was to examine the histological effects of Emdogain® on exposed tooth pulp of a Vietnamese pig (Sus scrofa verus).


METHODS
The study comprised 20 teeth of a Vietnamese pig. After class V preparation on the buccal surfaces of incisors, canines and first premolars, pulp was exposed. In the experimental group, the perforations were capped with Emdogain® (Straumann, Basel, Switzerland), while in the control group pulp capping was performed with MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass-ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). The observational period was 28 days, after which the animal was sacrificed and histological preparations were made. A light microscope was used to analyze dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp.


RESULTS
The formation of dentin bridge was observed in the experimental and control groups. Inflammation of the pulp was mild to moderate in both groups. Angiogenesis and many odontoblast-like cells, responsible for dentin bridge formation, were observed. Necrosis was not observed in any case, nor were bacteria present in the pulp.


CONCLUSIONS
Histological analysis indicated a favorable therapeutic effect of Emdogain® Gel in direct pulp capping of Vietnamese pigs. Pulp reaction was similar to that of MTA®.
Marijana Popović
MP
Bajić
Vesna
V
Danilović
Branislav
B
Prokić
Bogomir Bolka
BB
Prokić
Milica
M
Manojlović
Slavoljub
S
Živković
D000818:Animals; D003746:Dental Enamel Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D003782:Dental Pulp / Q000187:drug effects* / Q000502:physiology; D003785:Dental Pulp Capping / Q000379:methods*; D003789:Dental Pulp Exposure / Q000188:drug therapy; D003809:Dentin, Secondary / Q000187:drug effects; D004195:Disease Models, Animal; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D013552:Swine; D014945:Wound Healing / Q000502:physiology
D003746:Dental Enamel Proteins; D057872:Pulp Capping and Pulpectomy Agents; C109069:enamel matrix proteins