NFATC1 promotes cell growth and tumorigenesis in ovarian cancer up-regulating c-Myc through ERK1/2/p38 MAPK signal pathway.
It has been reported that nuclear factor of activated T cells (NFATC1) was up-regulated in cancers mediating malignant behaviors. However, the role of NFATC1 in ovarian cancer has not been elucidated. In the present study, we undertook to explore the clinicopathological significance of NFATC1 expression and the mechanism by which NFATC1 works in ovarian cancer. Expression status of NFATC1 was examined using immunohistochemistry. Both knockdown and re-expression of NFATC1 on ovarian cancer cells were employed to observe the effect overgrowth. It was found that NFATC1 was significantly overexpressed in ovarian cancer tissues in comparison with paired normal control tissues and that overexpression of NFATC1 was significantly associated with metastasis and poor prognosis on clinical tissue level. In in vitro ovarian cancer cell lines, we found that NFATC1 can promote proliferation up-regulating c-myc through activation of ERK1/2/p38/MAPK signal pathway. Together, the results we obtained demonstrated that NFATC1 played oncogenic role in ovarian cancer. Mechanistically, NFATC1 promoted growth of ovarian cancer cells up-regulating c-myc through activation of ERK1/2/p38/MAPK signal pathway, suggesting that NFATC1 might be used as a therapeutic target for ovarian cancer.
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Wenwen
W
Xu
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Junjie
J
Gu
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Qingling
Q
Ren
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Yanqiu
Y
Shi
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Qinhua
Q
Xia
Department of Gynaecology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 55 Hanzhong Road, Nanjing, 210029, China.
Jing
J
Wang
Department of Gynecologic Oncology, Jiangsu Cancer Hospital, Nanjing Medical University, 42 Baiziting, Nanjing, 210009, China.
Suli
S
Wang
Department of Gynecologic Oncology, Jiangsu Cancer Hospital, Nanjing Medical University, 42 Baiziting, Nanjing, 210009, China.
Yingchun
Y
Wang
Department of Gynecologic Oncology, Jiangsu Cancer Hospital, Nanjing Medical University, 42 Baiziting, Nanjing, 210009, China. NJMUGO_jhwang@163.com.
Jinhua
J
Wang
D000818:Animals; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020935:MAP Kinase Signaling System; D008819:Mice, Nude; D008875:Middle Aged; D050778:NFATC Transcription Factors / Q000502:physiology*; D009368:Neoplasm Transplantation; D018297:Neoplasms, Cystic, Mucinous, and Serous / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000378:metabolism; D011379:Prognosis; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D047368:Tumor Burden; D015854:Up-Regulation
C489427:MYC protein, human; D050778:NFATC Transcription Factors; C494003:NFATC1 protein, human; D016271:Proto-Oncogene Proteins c-myc
C-myc; ERK1/2; NFATC1; Ovarian cancer; Proliferation