Inhibitory effects of compounds from Styrax obassia on NO production.
Two new benzofurans, 2-(3,4-dimethoxyphenyl)-5-(1,3-dihydroxypropyl)-7-methoxybenzofuran (1) and 2-(3,4-methylenedioxyphenyl)-5-(3-hydroxymethyletoxy-1-hydroxypropyl)-7-methoxybenzofuran (2), a new triterpene, 3β, 6β, 21β-trihydroxyolean-12-ene (3), and eleven known compounds were isolated from the stem bark of Styrax obassia. The structures of the isolated compounds were established by extensive spectroscopic analyses, including 1D and 2D NMR and HRMS. Their anti-inflammatory activities were evaluated against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 macrophages. Compound 1 was shown to reduce LPS-induced iNOS expression in a dose-dependent manner. In addition, pretreating cells with 1 significantly suppressed their LPS-induced expression of COX-2 protein.
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Thao Quyen
TQ
Cao
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Manh Hung
MH
Tran
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
Jeong Ah
JA
Kim
College of Natural Science, Kangwon National University, Kangwon 200-701, Republic of Korea.
Phuong Thao
PT
Tran
College of Natural Science, Kangwon National University, Kangwon 200-701, Republic of Korea. Electronic address: jhlee36@kangwon.ac.kr.
Jeong-Hyung
JH
Lee
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea.
Mi Hee
MH
Woo
Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, Chungbuk 363-883, Republic of Korea.
Hyeong-Kyu
HK
Lee
College of Pharmacy, Drug Research and Development Center, Catholic University of Daegu, Gyeongbuk 712-702, Republic of Korea. Electronic address: bsmin@cu.ac.kr.
Byung Sun
BS
Min
D000818:Animals; D000840:Anisoles / Q000302:isolation & purification / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D024301:Plant Bark / Q000737:chemistry; D032362:Styrax / Q000737:chemistry*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology
C570118:2-(3,4-dimethoxyphenyl)-5-(3-methoxypropyl)benzofuran; D000840:Anisoles; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001572:Benzofurans; D052246:Cyclooxygenase 2 Inhibitors; D014315:Triterpenes; C000597557:homoegonol; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2
Benzofuran; NO production; RAW246.7 cells; Styracaceae; Styrax obassia