Near-IR mediated intracellular uncaging of NO from cell targeted hollow gold nanoparticles.
We demonstrate modulation of nitric oxide release in solution and in human prostate cancer cells from a thiol functionalized cupferron (TCF) absorbed on hollow gold nanoshells (HGNs) using near-infrared (NIR) light. NO release from the TCF-HGN conjugates occurs through localized surface heating due to NIR excitation of the surface plasmon. Specific HGN targeting is achieved through cell surface directed peptides, and excitation with tissue penetrating NIR light provides unprecedented spatio-temporal control of NO delivery to biological targets.
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106-9510 USA. reich@chem.ucsb.edu ford@chem.ucsb.edu.
Elizabeth S
ES
Levy
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106-9510 USA. reich@chem.ucsb.edu ford@chem.ucsb.edu.
Demosthenes P
DP
Morales
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106-9510 USA. reich@chem.ucsb.edu ford@chem.ucsb.edu.
John V
JV
Garcia
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106-9510 USA. reich@chem.ucsb.edu ford@chem.ucsb.edu.
Norbert O
NO
Reich
Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106-9510 USA. reich@chem.ucsb.edu ford@chem.ucsb.edu.
Peter C
PC
Ford
D016503:Drug Delivery Systems; D004705:Endocytosis / Q000502:physiology; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D057145:Nanoshells / Q000737:chemistry*; D039942:Neuropilin-1 / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000737:chemistry* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010455:Peptides / Q000737:chemistry; D010782:Photolysis; D011092:Polyethylene Glycols / Q000737:chemistry
D020030:Nitric Oxide Donors; D010455:Peptides; D039942:Neuropilin-1; D009569:Nitric Oxide; D011092:Polyethylene Glycols; D006046:Gold; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester