Generation and Analysis of Chromosomal Contact Maps of Yeast Species.
Genome-wide derivatives of the chromosome conformation capture (3C) technique are now well-established approaches to study the multiscale average organization of chromosomes from bacteria to mammals. However, the experimental parameters of the protocol have to be optimized for different species, and the downstream experimental products (i.e., pair-end sequences) are influenced by these parameters. Here, we describe a complete pipeline to generate 3C-seq libraries and compute chromosomal contact maps of yeast species.
Institut Pasteur, Department Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015, Paris, France.|CNRS, UMR 3525, 75015, Paris, France.
Axel
A
Cournac
Institut Pasteur, Department Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015, Paris, France.|CNRS, UMR 3525, 75015, Paris, France.
Martial
M
Marbouty
LPTMC, Université Pierre et Marie Curie, 4 Place Jussieu, 75005, Paris, France.
Julien
J
Mozziconacci
Institut Pasteur, Department Genomes and Genetics, Groupe Régulation Spatiale des Génomes, 75015, Paris, France. romain.koszul@pasteur.fr.|CNRS, UMR 3525, 75015, Paris, France. romain.koszul@pasteur.fr.
Romain
R
Koszul
D002843:Chromatin / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000235:genetics*; D016681:Genome, Fungal / Q000235:genetics*; D012441:Saccharomyces cerevisiae
D002843:Chromatin
3C; 3C analysis; Chromosome conformation capture; Genome assembly; Genome organization; Yeast