Use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect carbapenemase production in Enterobacteriaceae by a rapid meropenem degradation assay.
We evaluated the analytical performance of a liquid chromatography-tandem mass spectrometry assay to detect carbapenemase activity in a group of carbapenemase-producing Enterobacteriaceae by meropenem hydrolysis. This one-hour method showed a sensitivity of 94% and a specificity of 100%, representing a rapid and reliable option compared to conventional phenotypic assays.
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Claudio
C
Foschi
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Vincenzo
V
Franza
Central Laboratory - St. Orsola-Malpighi University Hospital, Bologna, Italy.
Matteo
M
Conti
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Maria Vittoria
MV
Tamburini
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Greta
G
Roncarati
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Miriam
M
Cordovana
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Viktoria
V
Smirnova
Central Laboratory - St. Orsola-Malpighi University Hospital, Bologna, Italy.
Daniela
D
Patrono
Central Laboratory - St. Orsola-Malpighi University Hospital, Bologna, Italy.
Rita
R
Mancini
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Maria Paola
MP
Landini
Unit of Microbiology, Department of Specialistic, Diagnostic and Experimental Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Simone
S
Ambretti
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D002853:Chromatography, Liquid / Q000379:methods*; D004755:Enterobacteriaceae / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D000077731:Meropenem; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013845:Thienamycins / Q000737:chemistry / Q000378:metabolism*; D001618:beta-Lactamases / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013845:Thienamycins; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem
Carbapenem resistance; Carbapenemase activity; Enterobacteriaceae; Liquid chromatography-tandem mass spectrometry; Meropenem hydrolysis