Ezh2 is involved in radial neuronal migration through regulating Reelin expression in cerebral cortex.
Radial migration of pyramidal neurons is an important event during the development of cerebral cortex. Neurons experience series of morphological and directional transitions to get to their final laminar positions. Here we report that the histone methyltransferase enhancer of zest homolog 2 (Ezh2) is involved in the regulation of cortical radial migration. We show that Ezh2 knockdown leads to disturbed neuronal orientation, which results in the impairment of radial migration. Further results reveal that this migration deficiency may be due to the derepression of Reelin transcription in the migrating neurons. Our study provides evidence that epigenetic regulation of Reelin by Ezh2 maintains appropriate Reelin expression pattern to fulfill proper orientation of migrating neurons.
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Linnan
L
Zhao
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Jun
J
Li
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Yuanlin
Y
Ma
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Jiutao
J
Wang
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Wen
W
Pan
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Kai
K
Gao
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Zhengrong
Z
Zhang
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Tianlan
T
Lu
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Yanyan
Y
Ruan
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Weihua
W
Yue
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Shanting
S
Zhao
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.
Lifang
L
Wang
Peking University Sixth Hospital/Institute of Mental Health, Beijing 100191, China.|National Clinical Research Center for Mental Disorders/Key Laboratory for Mental Health, Ministry of Health (Peking University), Beijing 100191, China.|Peking-Tsinghua Center for Life Sciences and PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China.|Shenzhen Key Laboratory for Neuronal Structural Biology, Shenzhen Peking University-Hong Kong University of Science and Technology Medical Center, Shenzhen 518035, China.
Dai
D
Zhang
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism*; D000071221:Enhancer of Zeste Homolog 2 Protein; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D063151:Polycomb Repressive Complex 2 / Q000502:physiology*; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism*
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2; C000717127:RELN protein, human; D012697:Serine Endopeptidases