3D Data Mapping and Real-Time Experiment Control and Visualization in Brain Slices.
Here, we propose two basic concepts that can streamline electrophysiology and imaging experiments in brain slices and enhance data collection and analysis. The first idea is to interface the experiment with a software environment that provides a 3D scene viewer in which the experimental rig, the brain slice, and the recorded data are represented to scale. Within the 3D scene viewer, the user can visualize a live image of the sample and 3D renderings of the recording electrodes with real-time position feedback. Furthermore, the user can control the instruments and visualize their status in real time. The second idea is to integrate multiple types of experimental data into a spatial and temporal map of the brain slice. These data may include low-magnification maps of the entire brain slice, for spatial context, or any other type of high-resolution structural and functional image, together with time-resolved electrical and optical signals. The entire data collection can be visualized within the 3D scene viewer. These concepts can be applied to any other type of experiment in which high-resolution data are recorded within a larger sample at different spatial and temporal coordinates.
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Marco A
MA
Navarro
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Jaime V K
JV
Hibbard
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Michael E
ME
Miller
Division of Biological Sciences, University of Missouri, Columbia, Missouri.
Tyler W
TW
Nivin
Division of Biological Sciences, University of Missouri, Columbia, Missouri. Electronic address: milescul@missouri.edu.
Lorin S
LS
Milescu
D000465:Algorithms; D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000502:physiology; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D005246:Feedback; D021621:Imaging, Three-Dimensional / Q000379:methods*; D020407:Internet; D008853:Microscopy / Q000379:methods; D009474:Neurons / Q000502:physiology; D061848:Optical Imaging / Q000379:methods; D012984:Software; D013997:Time Factors; D046509:Tissue Culture Techniques