Pin1 promotes prostate cancer cell proliferation and migration through activation of Wnt/β-catenin signaling.
BACKGROUND
Recent evidence suggests that the peptidyl-prolyl isomerase Pin1 is an oncoprotein that acts as a novel therapeutic target in a variety of tumors. In this study, we investigated the clinical significance of Pin1 and its function in prostate cancer (PCa) tumor progression.


METHODS
Immunohistochemical and quantitative RT-PCR analyses were performed to detect the expression of Pin1 in 86 PCa tissue samples. The functional role of Pin1 was evaluated by small interfering RNA-mediated depletion in PCa cells followed by analyses of cell proliferation and migration. Furthermore, the association between expression of Pin1 and levels of β-catenin and cyclin D1 was also evaluated.


RESULTS
Our results showed that the high expression of Pin1 staining was 66 of 86 (76.74 %) PCa samples, and in 25 of 86 (29.07 %) BPH tissues, the difference was statistically significant (P < 0.001). Pin1 was significantly elevated in all PCa cell lines when compared to the normal RWPE-1 cells. We observed that proliferation and migration of LNCaP cells were inhibited by Pin1 knockdown. The levels of β-catenin and cyclin D1 in clinical PCa specimens were positively associated with Pin1 expression.


CONCLUSIONS
Our results suggest that Pin1 plays an important role in tumorigenesis of PCa, suggesting that targeting Pin1 pathway could represent a potential modality for treating PCa.
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
Z
Z
Zhu
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
H
H
Zhang
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
F
F
Lang
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China. drzhuzj666@163.com.
G
G
Liu
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
D
D
Gao
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
B
B
Li
Department of Urology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Kuiwei District, Weifang, Shandong, China.
Y
Y
Liu
D015153:Blotting, Western; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D000072340:NIMA-Interacting Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D060449:Wnt Signaling Pathway / Q000502:physiology*
D000072340:NIMA-Interacting Peptidylprolyl Isomerase; C000606846:PIN1 protein, human
Cyclin D1; Pin1; Proliferation; Prostate cancer; Targeted therapy; β-catenin