Recent developments and applications of clickable photoprobes in medicinal chemistry and chemical biology.
Photoaffinity labeling is a well-known biochemical technique that has grown significantly since the turn of the century, principally due to its combination with bioorthogonal/click chemistry reactions. This review highlights new developments and applications of clickable photoprobes in medicinal chemistry and chemical biology. In particular, recent examples of clickable photoprobes for target identification, activity- or affinity-based protein profiling (ABPP or AfBPP), characterization of sterol- or lipid-protein interactions and characterization of ligand-binding sites are presented.
Division of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA 15282, USA.
David J
DJ
Lapinsky
Neuroscience Medicinal Chemistry & Chemical Biology, Pfizer Worldwide Research & Development, Cambridge, MA 02139, USA.
Douglas S
DS
Johnson
D001665:Binding Sites; D002626:Chemistry, Pharmaceutical; D057930:Click Chemistry; D008024:Ligands; D008055:Lipids / Q000737:chemistry; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects*; D019920:Photoaffinity Labels / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013261:Sterols / Q000737:chemistry
D008024:Ligands; D008055:Lipids; D015335:Molecular Probes; D019920:Photoaffinity Labels; D011506:Proteins; D013261:Sterols