Formation of photosystem II reaction centers that work as energy sinks in lichen symbiotic Trebouxiophyceae microalgae.
Lichens are poikilohydric symbiotic organisms that can survive in the absence of water. Photosynthesis must be highly regulated in these organisms, which live under continuous desiccation-rehydration cycles, to avoid photooxidative damage. Analysis of chlorophyll a fluorescence induction curves in the lichen microalgae of the Trebouxiophyceae Asterochloris erici and in Trebouxia jamesii (TR1) and Trebouxia sp. (TR9) phycobionts, isolated from the lichen Ramalina farinacea, shows differences with higher plants. In the presence of the photosynthetic electron transport inhibitor DCMU, the kinetics of Q(A) reduction is related to variable fluorescence by a sigmoidal function that approaches a horizontal asymptote. An excellent fit to these curves was obtained by applying a model based on the following assumptions: (1) after closure, the reaction centers (RCs) can be converted into "energy sink" centers (sRCs); (2) the probability of energy leaving the sRCs is very low or zero and (3) energy is not transferred from the antenna of PSII units with sRCs to other PSII units. The formation of sRCs units is also induced by repetitive light saturating pulses or at the transition from dark to light and probably requires the accumulation of reduced Q(A), as well as structural changes in the reaction centers of PSII. This type of energy sink would provide a very efficient way to protect symbiotic microalgae against abrupt changes in light intensity.
Departamento de Ciencias de la Vida, Universidad de Alcalá, Edificio de Ciencias, Campus externo, 28871, Alcalá de Henares, Madrid, Spain. alfredo.guera@uah.es.
Alfredo
A
Guéra
Departamento de Ciencias de la Vida, Universidad de Alcalá, Edificio de Ciencias, Campus externo, 28871, Alcalá de Henares, Madrid, Spain.|Botánica, ICBIBE, Facultad de Ciencias Biológicas, Universitat de València, C/Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
Francisco
F
Gasulla
Botánica, ICBIBE, Facultad de Ciencias Biológicas, Universitat de València, C/Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
Eva
E
Barreno
D001203:Ascomycota / Q000502:physiology; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000460:Chlorophyta / Q000378:metabolism* / Q000502:physiology; D004237:Diuron / Q000494:pharmacology; D008011:Lichens / Q000378:metabolism / Q000502:physiology*; D008027:Light; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D013559:Symbiosis / Q000502:physiology
D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D004237:Diuron; D000077194:Chlorophyll A
Asterochloris erici; Chlorophyll a fluorescence; Energy sinks; Fluorescence transient; PSII reaction center; Trebouxia