Cell-autonomous requirement for TCF1 and LEF1 in the development of Natural Killer T cells.
Natural killer T (NKT) cells develop from common CD4(+) CD8(+) thymocyte precursors. Transcriptional programs that regulate the development of NKT cells in the thymus development remain to be fully delineated. Here, we demonstrate a cell-intrinsic requirement for transcription factors TCF1 and LEF1 for the development of all subsets of NKT cells. Conditional deletion of TCF1 alone results in a substantial reduction in NKT cells. The remaining NKT cells are eliminated when TCF1 and LEF1 are both deleted. These data reveal an essential role for TCF1 and LEF1 in development of NKT cells.
Immune Cells and Inflammation Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States.
Rosa
R
Berga-Bolaños
Immune Cells and Inflammation Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States.
Wandi S
WS
Zhu
Department of Microbiology, Interdisciplinary Immunology Graduate Program, University of Iowa, Iowa City, IA 52242, United States.
Farrah C
FC
Steinke
Department of Microbiology, Interdisciplinary Immunology Graduate Program, University of Iowa, Iowa City, IA 52242, United States.
Hai-Hui
HH
Xue
Immune Cells and Inflammation Section, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States; Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, United States. Electronic address: Jyoti-Sen@nih.gov.
Jyoti Misra
JM
Sen
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D051538:Hepatocyte Nuclear Factor 1-alpha / Q000235:genetics*; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology; D060168:Thymocytes / Q000166:cytology
D051538:Hepatocyte Nuclear Factor 1-alpha; C496488:Hnf1a protein, mouse; C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1
LEF1; NKT cells; TCF1