Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase.
Poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogel discs were prepared under sub-zero temperatures. Gelatin was attached covalently on the p(HEMA-GMA) cryogel discs and reversible immobilization of horseradish peroxidase (HRP) was performed. The p(HEMA-GMA) cryogel discs were characterized by swelling tests, scanning electron microscopy, and surface area measurements. HRP immobilization capacity of p(HEMA-GMA)/gelatin cryogel discs was 24.8 mg/g. Removal of phenol from aqueous solutions was performed using HRP immobilized p(HEMA-GMA)/gelatin cryogel. It was observed that within 2 h of contact time, the percentage of phenol removal reaches up to 91% in the presence of H2O2.
a Department of Chemistry , Hacettepe University, Biochemistry Division , Beytepe , Ankara , Turkey .|b National Centre of Excellence in Analytical Chemistry, University of Sindh , Jamshoro , Sindh , Pakistan .
Rabel
R
Soomro
c Department of Biology , Hacettepe University, Molecular Biology Division, Beytepe , Ankara , Turkey , and.
Işık
I
Perçin
b National Centre of Excellence in Analytical Chemistry, University of Sindh , Jamshoro , Sindh , Pakistan .
Najma
N
Memon
d H.E.J. Research Institute of Chemistry, University of Karachi , Pakistan.
Muhammad
M
Iqbal Bhanger
a Department of Chemistry , Hacettepe University, Biochemistry Division , Beytepe , Ankara , Turkey .
Adil
A
Denizli
D059985:Cryogels / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives* / Q000737:chemistry*
D059985:Cryogels; D004800:Enzymes, Immobilized; D011102:Polyhydroxyethyl Methacrylate; D005780:Gelatin; D006735:Horseradish Peroxidase
cryogel; gelatin; glycidyl methacrylate; horseradish peroxidase; phenol