[Optimization of SFE-CO2 Extraction for Ursolic Acid from Punica granatum Peel by Plackett-Burman and Central Composite Design].
OBJECTIVE
The optimum extraction of ursolic acid from Punica granatum peel by SFE-CO2 was investigated.


METHODS
Based on the design of Plackett-Burman(PB), significant factors influencing the yield of ursolic acid in the operation process were filtered, with the extraction rate of ursolic acid as the index. The results obtained by steepest ascent method approximated the maximum area of ursolic acid yield. Then the Central Composite Design(CCD) design was used to carry on the response surface optimization of significant factors, getting a two order mathematical model affecting the ursolic acid yield, as well as the optimum process conditions.


RESULTS
The best technological conditions of the extraction of ursolic acid from Punica granatum peel by SFE-CO2 were that the extraction temperature was 46. 29 °C, extraction time was 91. 6 min and the extraction pressure was 34. 49 MPa. Under the optimal conditions, verification test of ursolic acid yield was 12. 508 mg/g, approximating to the predicted value of 12. 645 mg/g.


CONCLUSIONS
The PB test and CCD test design are combined to optimize the extraction process of ursolic acid from Punica granatum peel by SFE-CO2. The screening results are statistically significant and the process operation is feasible.
Zhan-yi
ZY
Wang
Mei-hua
MH
Jin
Yu-hai
YH
Wang
Li-hua
LH
Zhang
Hai-dan
HD
Bi
Zhuo-wa
ZW
Li
D025924:Chromatography, Supercritical Fluid; D029561:Lythraceae / Q000737:chemistry*; D011312:Pressure; D013696:Temperature; D014315:Triterpenes / Q000302:isolation & purification*; D000097245:Ursolic Acid
D014315:Triterpenes; D000097245:Ursolic Acid