The eukaryotic translation initiation factor 3f (eIF3f) interacts physically with the alpha 1B-adrenergic receptor and stimulates adrenoceptor activity.
BACKGROUND
eIF3f is a multifunctional protein capable of interacting with proteins involved in different cellular processes, such as protein synthesis, DNA repair, and viral mRNA edition. In human cells, eIF3f is related to cell cycle and proliferation, and its deregulation compromises cell viability.


RESULTS
We here report that, in native conditions, eIF3f physically interacts with the alpha 1B-adrenergic receptor, a plasma membrane protein considered as a proto-oncogene, and involved in vasoconstriction and cell proliferation. The complex formed by eIF3f and alpha 1B-ADR was found in human and mouse cell lines. Upon catecholamine stimulation, eIF3f promotes adrenoceptor activity in vitro, independently of the eIF3f proline- and alanine-rich N-terminal region.


CONCLUSIONS
The eIF3f/alpha adrenergic receptor interaction opens new insights regarding adrenoceptor-related transduction pathways and proliferation control in human cells. The eIf3f/alpha 1B-ADR complex is found in mammals and is not tissue specific.
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-3 Ciudad Universitaria Avenida Francisco J. Múgica S/N, Morelia, Michoacán, 58030, México. mcmariojav@hotmail.com.|Present address: Universidad Tecnológica de Morelia, Morelia, Michoacán, 58200, México. mcmariojav@hotmail.com.
Mario Javier
MJ
Gutiérrez-Fernández
División de Estudios de Posgrado de la Facultad de Ciencias Médicas y Biológicas "Dr. Ignacio Chávez", Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, 58020, México. higareda@umich.mx.
Ana Edith
AE
Higareda-Mendoza
Present address: Universidad Tecnológica de Morelia, Morelia, Michoacán, 58200, México. qfb.cagc@gmail.com.|División de Estudios de Posgrado de la Facultad de Ciencias Médicas y Biológicas "Dr. Ignacio Chávez", Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, 58020, México. qfb.cagc@gmail.com.
César Adrián
CA
Gómez-Correa
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B-3 Ciudad Universitaria Avenida Francisco J. Múgica S/N, Morelia, Michoacán, 58030, México. mapardo@umich.mx.
Marco Aurelio
MA
Pardo-Galván
D000595:Amino Acid Sequence; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D045744:Cell Line, Tumor; D039621:Eukaryotic Initiation Factor-3 / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D000090063:Proto-Oncogene Mas; D018340:Receptors, Adrenergic, alpha-1 / Q000737:chemistry / Q000378:metabolism*
D002395:Catecholamines; C584155:EIF3F protein, human; D039621:Eukaryotic Initiation Factor-3; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D018340:Receptors, Adrenergic, alpha-1