Poly-adenine-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes.
Enzyme complexes are assembled at the two-dimensional lipid membrane or prearranged on three-dimensional scaffolding proteins to regulate their catalytic activity in cells. Inspired by nature, we have developed gold nanoparticle-based spherical DNAzymes (SNAzymes) with programmably engineered activities by exploiting poly-adenine (polyA)-Au interactions. In a SNAzyme, AuNPs serve as the metal core, which is decorated with a functional shell of DNAzymes. Conventional thiolated DNAzyme-based assembly leads to disordered structures with suppressed activity. In contrast, by using an anchoring block of polyA tails, we find that the activity of SNAzymes can be programmably regulated. By using a polyA30 tail, SNAzymes demonstrated remarkably enhanced binding affinity compared to the thiolated DNAzyme-based assembly (∼75-fold) or individual DNAzymes in the solution phase (∼10-fold). More significantly, this increased affinity is directly translated to the sensitivity improvement in the SNAzyme-based lead sensor. Hence, this design of SNAzymes may provide new opportunities for developing biosensors and bioimaging probes for theranostic applications.
Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. zuoxiaolei@sinap.ac.cn.
Dan
D
Zhu
Hao
H
Pei
Jie
J
Chao
Shao
S
Su
Ali
A
Aldalbahi
Mostafizur
M
Rahaman
Lihua
L
Wang
Lianhui
L
Wang
Wei
W
Huang
Chunhai
C
Fan
Xiaolei
X
Zuo
D000241:Adenosine / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D021881:DNA, Catalytic / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
D021881:DNA, Catalytic; D011108:Polymers; C069424:polyadenosine; D006046:Gold; D000241:Adenosine