Expanding the scope of alkyne-mediated bioconjugations utilizing unnatural amino acids.
The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs into green fluorescent protein, and optimized a novel Cadiot-Chodkiewicz bioconjugation, effectively expanding the toolbox of chemical reactions that can be employed in the preparation of bioconjugates.
Department of Chemistry, College of William & Mary, Williamsburg, Virginia 23187, USA. dyoung01@wm.edu.
Johnathan C
JC
Maza
Department of Chemistry, College of William & Mary, Williamsburg, Virginia 23187, USA. dyoung01@wm.edu.
Zachary M
ZM
Nimmo
Department of Chemistry, College of William & Mary, Williamsburg, Virginia 23187, USA. dyoung01@wm.edu.
Douglas D
DD
Young
D000480:Alkynes / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008958:Models, Molecular
D000480:Alkynes; D000596:Amino Acids; D049452:Green Fluorescent Proteins