Perilla frutescens Extract Ameliorates Acetylcholinesterase and Trimethyltin Chloride-Induced Neurotoxicity.
One of the critical features of Alzheimer's disease is cognitive dysfunction, which is, in part, due to decreases in acetylcholine (ACh). The ethanol extract of Perilla frutescens was selected for isolating the acetylcholinesterase (AChE) inhibitor based on preliminary screening. In vivo behavioral tests were performed to examine the effects of the P. frutescens extract on trimethyltin chloride-induced impairment of learning and memory in mice. A diet containing P. frutescens extract effectively reversed learning and memory impairment on the Y-maze and passive avoidance tests. To isolate the active compound from the P. frutescens extract, solvent partitioning, silica gel open column chromatography, thin-layer chromatography, and high-performance liquid chromatography were used. The AChE inhibitor was identified as rosmarinic acid.
1 Functional Food Research Center, Korea University , Seoul, Korea.
Soo Jung
SJ
Choi
2 Department of Food and Biotechnology, Korea University , Seoul, Korea.
Seung Sang
SS
Oh
1 Functional Food Research Center, Korea University , Seoul, Korea.
Cho Rong
CR
Kim
2 Department of Food and Biotechnology, Korea University , Seoul, Korea.
Yoon Kyung
YK
Kwon
3 National Institute of Food and Drug Safety Evaluation , Ministry of Food and Drug Safety, Chungbuk, Korea.
Soo Hwan
SH
Suh
4 Department of Food Science and Nutrition, University of Minnesota , St. Paul, Minnesota, USA .
Jae Kyeom
JK
Kim
5 Department of Food Science and Biotechnology, Gachon University , Gyeonggi, Korea.
Gwi Gun
GG
Park
6 Department of Microbiology, Dankook University , Chungnam, Korea.
Seung-Yeol
SY
Son
2 Department of Food and Biotechnology, Korea University , Seoul, Korea.
Dong-Hoon
DH
Shin
D000110:Acetylcholinesterase / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000201:enzymology / Q000523:psychology; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D008297:Male; D018782:Maze Learning; D051379:Mice; D008813:Mice, Inbred ICR; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D014298:Trimethyltin Compounds / Q000633:toxicity*
D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D014298:Trimethyltin Compounds; C040533:trimethyltin chloride; D000110:Acetylcholinesterase
Acetylcholinesterase; Alzheimer's disease; Perilla frutescens; Rosmarinic acid; trimethyltin chloride