Chemical constituents from Hericium erinaceus and their ability to stimulate NGF-mediated neurite outgrowth on PC12 cells.
One new meroterpenoid, named hericenone K (11), along with 10 known compounds (1-10), ergosterol peroxide (1), cerevisterol (2), 3β,5α,9α-trihydroxy-ergosta-7,22-dien-6-one (3), inoterpene A (4), astradoric acid C (5), betulin (6), oleanolic acid (7), ursolic acid (8), hemisceramide (9), and 3,4-dihydro-5-methoxy-2-methyl-2-(4'-methyl-2'-oxo-3'-pentenyl)-9(7H)-oxo-2H-furo[3,4-h]benzopyran (10), was isolated from the fruiting bodies of the mushroom Hericium erinaceus. Their structures were characterized on the basis of spectroscopic methods, as well as through comparison with previously reported data. Compounds 3-6, 8, and 9 were isolated from Hericium species for the first time. Compounds 10 and 11 was suggested to be racemic by the CD spectrum data and specific rotations, which ware resolved by chiral HPLC into respective enantiomers. Compounds 1-3, (±)-10, (-)-10 and (+)-10 in the presence of NGF (20 ng/mL) exerted a significant increase in neurite-bearing cells.
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
Cheng-Chen
CC
Zhang
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
Xia
X
Yin
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
Chen-Yu
CY
Cao
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
Jing
J
Wei
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China.
Qiang
Q
Zhang
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, People's Republic of China. Electronic address: jinminggao@nwsuaf.edu.cn.
Jin-Ming
JM
Gao
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002518:Ceramides / Q000302:isolation & purification / Q000494:pharmacology; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D039821:Monoterpenes / Q000302:isolation & purification / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D016716:PC12 Cells; D010840:Phytosterols / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D013237:Stereoisomerism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology
D002518:Ceramides; D039821:Monoterpenes; D009414:Nerve Growth Factors; D010840:Phytosterols; D014315:Triterpenes; C479308:hemisceramide; C006954:cerevisterol; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol
Hericium erinaceus; Meroterpenoid; NGF-mediated PC12 cells; Natural products; Neurite outgrowth-promoting activity; Neuroactives; Prenylated benzopyran; TDDFT