Evaluation of enzyme immobilization methods for paper-based devices--A glucose oxidase study.
Paper-based sensors gained almost explosive attention during the last few years. A large number of systems, often destined to resource limited settings is based on enzymatic reactions. Choice of an adequate immobilization method could significantly prolong the shelf-life of such sensors, especially in applications, where exposure to high temperatures during storage and transport is more than a threat. We are seeking to compare a variety of immobilization methods based on different phenomena (adsorption, entrapment in gel, microencapsulation, covalent linkage), with total of 33 methods tested. Glucose oxidase was used as a model enzyme. Enzymatic activity of immobilized samples was accompanied for a period of 24 weeks considering two sets of samples, one stored in 4 °C and other in ambient temperature.
Department of Analytical Chemistry, Institute of Chemistry-UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil; National Institute of Science and Technology in Bioanalytics, Institute of Chemistry-UNICAMP, P.O. Box 6154, Campinas, Brazil. Electronic address: emilia.nery@iqm.unicamp.br.
Emilia Witkowska
EW
Nery
Department of Analytical Chemistry, Institute of Chemistry-UNICAMP, P.O. Box 6154, 13084-971 Campinas, SP, Brazil; National Institute of Science and Technology in Bioanalytics, Institute of Chemistry-UNICAMP, P.O. Box 6154, Campinas, Brazil. Electronic address: kubota@iqm.unicamp.br.
Lauro T
LT
Kubota
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards*; D004800:Enzymes, Immobilized / Q000032:analysis*; D005949:Glucose Oxidase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D010209:Paper
D004800:Enzymes, Immobilized; D005949:Glucose Oxidase
Bioactive paper; Cellulose; Enzyme immobilization; Lab-on-paper; Paper-based analytical device