A high-throughput liquid bead array-based screening technology for Bt presence in GMO manipulation.
The number of species and planting areas of genetically modified organisms (GMOs) has been rapidly developed during the past ten years. For the purpose of GMO inspection, quarantine and manipulation, we have now devised a high-throughput Bt-based GMOs screening method based on the liquid bead array. This novel method is based on the direct competitive recognition between biotinylated antibodies and beads-coupled antigens, searching for Bt presence in samples if it contains Bt Cry1 Aa, Bt Cry1 Ab, Bt Cry1 Ac, Bt Cry1 Ah, Bt Cry1 B, Bt Cry1 C, Bt Cry1 F, Bt Cry2 A, Bt Cry3 or Bt Cry9 C. Our method has a wide GMO species coverage so that more than 90% of the whole commercialized GMO species can be identified throughout the world. Under our optimization, specificity, sensitivity, repeatability and availability validation, the method shows a high specificity and 10-50 ng/mL sensitivity of quantification. We then assessed more than 1800 samples in the field and food market to prove capacity of our method in performing a high throughput screening work for GMO manipulation. Our method offers an applicant platform for further inspection and research on GMO plants.
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Wei
W
Fu
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Huiyu
H
Wang
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Chenguang
C
Wang
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Lin
L
Mei
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China.
Xiangmei
X
Lin
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China. Electronic address: xqhancaiq@163.com.
Xueqing
X
Han
Chinese Academy of Inspection and Quarantine, Beijing Economic-Technological Development Area, Daxing District, Beijing 100176, PR China. Electronic address: zhusf@caiq.gov.cn.
Shuifang
S
Zhu
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D030841:Food, Genetically Modified / Q000145:classification*; D006460:Hemolysin Proteins / Q000032:analysis*; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Bt presence; GMO manipulation; High-throughput; Liquid bead array; Screening method