Rapid Hepatobiliary Excretion of Micelle-Encapsulated/Radiolabeled Upconverting Nanoparticles as an Integrated Form.
In the field of nanomedicine, long term accumulation of nanoparticles (NPs) in the mononuclear phagocyte system (MPS) such as liver is the major hurdle in clinical translation. On the other hand, NPs could be excreted via hepatobiliary excretion pathway without overt tissue toxicity. Therefore, it is critical to develop NPs that show favorable excretion property. Herein, we demonstrated that micelle encapsulated (64)Cu-labeled upconverting nanoparticles (micelle encapsulated (64)Cu-NOTA-UCNPs) showed substantial hepatobiliary excretion by in vivo positron emission tomography (PET) and also upconversion luminescence imaging (ULI). Ex vivo biodistribution study reinforced the imaging results by showing clearance of 84% of initial hepatic uptake in 72 hours. Hepatobiliary excretion of the UCNPs was also verified by transmission electron microscopy (TEM) examination. Micelle encapsulated (64)Cu-NOTA-UCNPs could be an optimal bimodal imaging agent owing to quantifiability of (64)Cu, ability of in vivo/ex vivo ULI and good hepatobiliary excretion property.
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.|Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Hyo Jung
HJ
Seo
Laboratory for Advanced Molecular Probing (LAMP), Research Center for Convergence Nanotechnology, Korea Research Institute of Chemical Technology, Daejeon, Korea.
Sang Hwan
SH
Nam
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.|Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Hyung-Jun
HJ
Im
Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Ji-yong
JY
Park
Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Ji Youn
JY
Lee
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Korea.|School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Byeongjun
B
Yoo
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.|Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Yun-Sang
YS
Lee
Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Jae Min
JM
Jeong
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Korea.|School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Taeghwan
T
Hyeon
Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Ji Who
JW
Kim
Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Jae Sung
JS
Lee
Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine, Seoul, Korea.
In-Jin
IJ
Jang
Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine, Seoul, Korea.
Joo-Youn
JY
Cho
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.|Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Do Won
DW
Hwang
Laboratory for Advanced Molecular Probing (LAMP), Research Center for Convergence Nanotechnology, Korea Research Institute of Chemical Technology, Daejeon, Korea.|School of Chemical Engineering, Sungkyunkwan University, Suwon, Korea.
Yung Doug
YD
Suh
Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Korea.|Department of Nuclear medicine, Seoul National University College of Medicine,Seoul, Korea.
Dong Soo
DS
Lee
D000818:Animals; D001659:Biliary Tract / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008823:Micelles; D053758:Nanoparticles; D049268:Positron-Emission Tomography
D008823:Micelles