Effect of White Kidney Beans (Phaseolus vulgaris L. var. Beldia) on Small Intestine Morphology and Function in Wistar Rats.
The chronic ingestion of raw or undercooked kidney beans (Phaseolus vulgaris L.) causes functional and morphological derangement in various tissues. The major objectives of this study were to investigate the gavage effects of a raw Beldia bean variety that is widely consumed in Tunisia, on the small intestine morphology and jejunal absorption of water, electrolytes, and glucose in Wistar rats. Twenty young male rats were randomly divided into two groups of 10 rats. The first group served as the control and was gavaged with 300 mg of a rodent pellet flour suspension (RPFS), whereas the second experimental group was challenged with 300 mg of a Beldia bean flour suspension (BBFS) for 10 days. Histological studies were performed using light and electron microcopy. The intestinal transport of water, sodium, potassium, and glucose was studied by perfusing the jejunal loops of the small bowels in vivo. The feeding experiments indicated that BBFS did not affect weight gain. Histomorphometric analyses showed that the villus heights, crypt depths, and crypt/villus ratios in the jejunum and ileum were greater in the BBFS-fed rats than controls. Electron microscopy studies demonstrated that the rats exposed to RPFS exhibited intact intestinal tracts; however, the BBFS-treated rats demonstrated intestinal alterations characterized by abnormal microvillus architectures, with short and dense or long and slender features, in addition to the sparse presence of vesicles near the brush border membrane. BBFS administration did not significantly affect glucose absorption. However, significant decreases were observed in water and electrolyte absorption compared with the uptake of the controls. In conclusion, raw Beldia beans distorted jejunum morphology and disturbed hydroelectrolytic flux.
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .|2 Department of Animal Resources, Fisheries, and Food Technology, National Institute of Agronomy of Tunisia , El Mahrajène, Tunis, Tunisia .|3 School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education , Cheonan, Chungnam, Korea.
Nader
N
Nciri
3 School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education , Cheonan, Chungnam, Korea.
Namjun
N
Cho
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Nacef
N
Bergaoui
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Faiçal
F
El Mhamdi
4 Common Services Unit on Transmission Electron Microscopy, Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Aouatef
A
Ben Ammar
5 Clinical Biochemistry Laboratory, Salah Azaiez Institute of Carcinology , Bab Saâdoun, Tunis, Tunisia .
Najoua
N
Trabelsi
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Sami
S
Zekri
5 Clinical Biochemistry Laboratory, Salah Azaiez Institute of Carcinology , Bab Saâdoun, Tunis, Tunisia .
Fathi
F
Guémira
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Abderraouf
A
Ben Mansour
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Fayçal Haj
FH
Sassi
1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Bab Saâdoun, Tunis, Tunisia .
Fatma
F
Ben Aissa-Fennira
D000818:Animals; D003296:Cooking; D004573:Electrolytes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008871:Microvilli / Q000187:drug effects; D027805:Phaseolus / Q000737:chemistry*; D010835:Phytohemagglutinins / Q000009:adverse effects; D010936:Plant Extracts / Q000009:adverse effects*; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity; D014416:Tunisia
D004573:Electrolytes; D010835:Phytohemagglutinins; D010936:Plant Extracts; D005947:Glucose
bean ( Phaseolus vulgaris L.); electrolyte transport; glucose transport; phytohemagglutinin; small intestine morphology; white kidney