Heme Oxygenase-1 Suppresses Bovine Viral Diarrhoea Virus Replication in vitro.
Viral cycle progression depends upon host-cell processes in infected cells, and this is true for bovine viral diarrhoea virus (BVDV), the causative agent of BVD that is a worldwide threat to the bovine industry. Heme oxygenase-1 (HO-1) is a ubiquitously expressed inducible isoform of the first and rate-limiting enzyme for heme degradation. Recent studies have demonstrated that HO-1 has significant antiviral properties, inhibiting the replication of viruses such as ebola virus, human immunodeficiency virus, hepatitis C virus, and porcine reproductive and respiratory syndrome virus. However, the function of HO-1 in BVDV infection is unclear. In the present study, the relationship between HO-1 and BVDV was investigated. In vitro analysis of HO-1 expression in BVDV-infected MDBK cells demonstrated that a decrease in HO-1 as BVDV replication increased. Increasing HO-1 expression through adenoviral-mediated overexpression or induction with cobalt protoporphyrin (CoPP, a potent HO-1 inducer), pre- and postinfection, effectively inhibited BVDV replication. In contrast, HO-1 siRNA knockdown in BVDV-infected cells increased BVDV replication. Therefore, the data were consistent with HO-1 acting as an anti-viral factor and these findings suggested that induction of HO-1 may be a useful prevention and treatment strategy against BVDV infection.
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Chong
C
Zhang
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Fengxing
F
Pu
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Angke
A
Zhang
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Lele
L
Xu
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Na
N
Li
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Yunhuan
Y
Yan
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Jiming
J
Gao
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Hongliang
H
Liu
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
Gaiping
G
Zhang
Division of Virology, Department of Pathology, University of Cambridge, Hills Road, Cambridge, CB2 2QQ, United Kingdom.
Ian G
IG
Goodfellow
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
En-Min
EM
Zhou
College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.|Experimental Station of Veterinary Pharmacology and Veterinary Biotechnology, Ministry of Agriculture, China, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.
Shuqi
S
Xiao
D000818:Animals; D002417:Cattle; D002460:Cell Line; D001908:Diarrhea Viruses, Bovine Viral / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D014779:Virus Replication / Q000502:physiology*
D051547:Heme Oxygenase-1