Alginate/Poly(γ-glutamic Acid) Base Biocompatible Gel for Bone Tissue Engineering.
A technique for synthesizing biocompatible hydrogels by cross-linking calcium-form poly(γ-glutamic acid), alginate sodium, and Pluronic F-127 was created, in which alginate can be cross-linked by Ca(2+) from Ca-γ-PGA directly and γ-PGA molecules introduced into the alginate matrix to provide pH sensitivity and hemostasis. Mechanical properties, swelling behavior, and blood compatibility were investigated for each hydrogel compared with alginate and for γ-PGA hydrogel with the sodium form only. Adding F-127 improves mechanical properties efficiently and influences the temperature-sensitive swelling of the hydrogels but also has a minor effect on pH-sensitive swelling and promotes anticoagulation. MG-63 cells were used to test biocompatibility. Gelation occurred gradually through change in the elastic modulus as the release of calcium ions increased over time and caused ionic cross-linking, which promotes the elasticity of gel. In addition, the growth of MG-63 cells in the gel reflected nontoxicity. These results showed that this biocompatible scaffold has potential for application in bone materials.
Department of Radiology, Wan Fang Hospital, Taipei Medical University, Taipei 110, Taiwan ; Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
Wing P
WP
Chan
Department of Health Healing and Health Marketing, Kainan University, Taoyuan 338, Taiwan.
Fu-Chen
FC
Kung
Department of Mechanical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Yu-Lin
YL
Kuo
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Ming-Chen
MC
Yang
Graduate Institute of Clinical Medicine and Graduate Institute of Medical Sciences and Graduate Institute of Biomaterials, Taipei Medical University, Taipei 110, Taiwan.
Wen-Fu Thomas
WF
Lai
D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology; D018786:Bone Substitutes / Q000138:chemical synthesis* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds
D000464:Alginates; D001672:Biocompatible Materials; D018786:Bone Substitutes; D006603:Hexuronic Acids; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D020723:Glucuronic Acid