Identification of the Critical Site of Calponin 1 for Suppression of Ovarian Cancer Properties.
BACKGROUND
Although several studies have demonstrated the tumor suppressive function of CNN1 (calponin 1), no studies have performed a site-specific analysis of CNN1 on tumor cell activities.


METHODS
We herein studied the site-specific effects of CNN1 in ovarian cancer cells using full-length CNN1 (fCNN1), three CNN1 repeats (3CNRs), or the first CNN1 repeat (CNR1) expression vectors. Ovarian cancer cells stably expressing each construct were analyzed for in vitro proliferation, cell motility, invasion, and soft agar assays. An in vitro model of pleural dissemination was also established.


RESULTS
Cell proliferation, anchorage-independent colony formation, cell motility, and cell invasion were all suppressed in fCNN1, 3CNRs, and CNR1-stably-expressing cells. CNN1 expression in mesothelial cells suppressed cancer cell invasion into a monolayer of mesothelial cells.


CONCLUSIONS
CNR1 showed similar suppressive effects as fCNN1. Results suggest CNR1 as a potential small synthetic peptide candidate for therapeutic strategies against ovarian cancer.
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Takako
T
Yamane
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan asanoma@med.kyushu-u.ac.jp.
Kazuo
K
Asanoma
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kagoshima University, Kagoshima, Japan.
Hiroaki
H
Kobayashi
Research Center for Environment and Developmental Medical Sciences, Kyushu University, Fukuoka, Japan.
Ge
G
Liu
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Hiroshi
H
Yagi
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Tatsuhiro
T
Ohgami
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Akimasa
A
Ichinoe
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Kenzo
K
Sonoda
Research Center for Environment and Developmental Medical Sciences, Kyushu University, Fukuoka, Japan.
Norio
N
Wake
Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
Kiyoko
K
Kato
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*; D000096985:Calponins
D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D000096985:Calponins
Calponin 1 (CNN1); F-actin; cell invasion; cell motility