Electrochemical impedance based chiral analysis of anti-ascorbutic drug: l-Ascorbic acid and d-ascorbic acid using C-dots decorated conductive polymer nano-composite electrode.
Clinical manifestations owing to l-ascorbic acid for scurvy as comparison to d-ascorbic acid and challenges of chiral purity are overcome by using chiral selective conductive polymer nanocomposite which mimics antibodies and enzymes. A novel chiral selective imprinted polyaniline-ferrocene-sulfonic acid film has been electrochemically fabricated on C-dots modified pencil graphite electrode. The performance of the obtained l-ascorbic acid or d-ascorbic acid chiral selective sensor was investigated by electrochemical impedance spectroscopy, cyclic and differential pulse voltammetry. The surface characteristics of the C-dots, chiral sensor before and after the de-doping of chiral d- and l-ascorbic acid were characterized by scanning electron microscopy, Raman spectroscopy and X-ray diffraction spectroscopy. Excellent recognition results were obtained by difference in electron transfer resistance. The proposed chiral sensor is capable of measuring d-ascorbic acid or l-ascorbic acid in aqueous as well as in real and commercial samples within the range of 0.020-0.187 nM and 0.003-0.232 nM with detection limit of 0.00073 nM and 0.00016 nM, respectively. The proposed method has also been examined for the chiral selective recognition of ascorbic acid isomers (d- and l-) quantitatively, in complicated matrices of real samples.
Complex Systems Group, Department of Chemistry, University of Delhi, Delhi 110007, India.
Indu
I
Pandey
Complex Systems Group, Department of Chemistry, University of Delhi, Delhi 110007, India. Electronic address: rama_kant_99@yahoo.com.
Rama
R
Kant
D001205:Ascorbic Acid / Q000032:analysis* / Q000737:chemistry; D002244:Carbon / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007536:Isomerism; D008839:Microelectrodes; D054802:Molecular Imprinting / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011108:Polymers; D002244:Carbon; D001205:Ascorbic Acid
Charge transfer resistance; Constant phase element; D-and l-ascorbic acid; Electrochemical impedance spectroscopy