Postnatal overnutrition in mice leads to impaired pulmonary mechanics in response to salbutamol.
Obesity increases the risk of respiratory disease, which is associated with airway hyperresponsiveness. Although the molecular underpinnings of this phenomenon are not well established, lung remodeling is known as an important factor in this process and could potentially explain compromised lung functions. In the present study, the obesity was induced by postnatal overnutrition in Swiss mice and we investigated the pulmonary mechanics after aerosolization of saline, methacholine, and salbutamol. The lungs were prepared for morphometric analysis. Obese animals showed bronchoconstriction in response to methacholine, as evidenced by airway and tissue resistance, tissue elastance, and hysteresivity. Salbutamol was effective at recovering the response only for airway resistance but not for tissue mechanics. We suggest that this impaired response in obese mice is related to collapsed alveolar, to inflammatory cells, and to elevated deposition collagen fibers in parenchymal tissue.
Department of Physiology, Institute of Biomedical Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
Vanessa P
VP
Teixeira
Department of Physiology, Institute of Biomedical Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
Daniela A B
DA
Cervilha
Department of Physiology, Institute of Biomedical Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
Layla D M
LD
Cabral
Department of Pharmacology, Institute of Biomedical Science, University of São Paulo, 05508-000, São Paulo, SP, Brazil.
Luiz M
LM
Oliveira
Department of Physiology, Institute of Biomedical Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
Erika K
EK
Incerpi
Integrative Animal Biology Laboratory, Institute of Biomedical Science, Federal University of Alfenas, 37130-000, Alfenas, MG, Brazil.
Rômulo D
RD
Novaes
Integrative Animal Biology Laboratory, Institute of Biomedical Science, Federal University of Alfenas, 37130-000, Alfenas, MG, Brazil.
Marisa
M
Ionta
Department of Physiology, Institute of Biomedical Science, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil. soncinir@yahoo.com.br.
Roseli
R
Soncini
D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001993:Bronchodilator Agents / Q000494:pharmacology*; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D009765:Obesity / Q000150:complications; D044343:Overnutrition / Q000503:physiopathology*; D012143:Respiratory Physiological Phenomena / Q000187:drug effects
D001993:Bronchodilator Agents; D000420:Albuterol
Bronchoconstrictor; Bronchodilator; Extracellular matrix; Overnutrition; Tissue resistance