Characterization of Lamprey IL-17 Family Members and Their Receptors.
IL-17 is an ancient cytokine implicated in a variety of immune defense reactions. We identified five members of the sea lamprey IL-17 family (IL-17D.1, IL-17D.2, IL-17E, IL-17B, and IL-17C) and six IL-17R genes (IL-17RA.1, IL-17RA.2, IL-17RA.3, IL-17RF, IL-17RE/RC, and IL-17RD), determined their relationship with mammalian orthologs, and examined their expression patterns and potential interactions to explore their roles in innate and adaptive immunity. The most highly expressed IL-17 family member is IL-17D.1 (mammalian IL-17D like), which was found to be preferentially expressed by epithelial cells of skin, intestine, and gills and by the two types of lamprey T-like cells. IL-17D.1 binding to rIL-17RA.1 and to the surface of IL-17RA.1-expressing B-like cells and monocytes of lamprey larvae was demonstrated, and treatment of lamprey blood cells with rIL-17D.1 protein enhanced transcription of genes expressed by the B-like cells. These findings suggest a potential role for IL-17 in coordinating the interactions between T-like cells and other cells of the adaptive and innate immune systems in jawless vertebrates.
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Qifeng
Q
Han
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Sabyasachi
S
Das
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Masayuki
M
Hirano
Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
Stephen J
http://orcid.org/0000-0003-2999-9690
SJ
Holland
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Nathanael
N
McCurley
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Peng
P
Guo
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and.
Charles S
CS
Rosenberg
Department of Developmental Immunology, Max-Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
Thomas
T
Boehm
Emory Vaccine Center, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322; and max.cooper@emory.edu.
Max D
MD
Cooper
D056704:Adaptive Immunity / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D020381:Interleukin-17 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D064094:Interleukin-27 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D048428:Petromyzon / Q000276:immunology*; D010802:Phylogeny; D011485:Protein Binding / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D053722:Receptors, Interleukin-17 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D059467:Transcriptome / Q000235:genetics
D000911:Antibodies, Monoclonal; D020381:Interleukin-17; D064094:Interleukin-27; D012333:RNA, Messenger; D053722:Receptors, Interleukin-17