Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi).
A Gram-stain-negative, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, RA1-3T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA1-3T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1-3T clustered with the type strains of three species of the genus Octadecabacter, showing 97.54-98.41 % 16S rRNA gene sequence similarity. Sequence similarities to other recognized species were less than 96.97 %. Strain RA1-3T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain RA1-3T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA1-3T was 56 mol% and DNA-DNA relatedness values with the type strains of Octadecabacter temperatus, Octadecabacter antarcticus and Octadecabacter arcticus were 13-24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RA1-3T is separated from other recognized species of the genus Octadecabacter. On the basis of the data presented, strain RA1-3T is considered to represent a novel species of the genus Octadecabacter, for which the name Octadecabacter ascidiaceicola sp. nov. is proposed. The type strain is RA1-3T ( = KCTC 42605T = CECT 8868T).
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
Young-Ok
YO
Kim
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
In-Suk
IS
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Sooyeon
S
Park
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
Bo-Hye
BH
Nam
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Ji-Min
JM
Park
Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan 619-705, South Korea.
Dong-Gyun
DG
Kim
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Jung-Hoon
JH
Yoon
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry; D014561:Urochordata / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10