The role of protein oxidation and DNA damage in elderly hypertension.
BACKGROUND
This study aimed to evaluate the role of protein oxidation and DNA damage in the elderly hypertensive (HT) patients.


METHODS
This study consisted of four groups: two elderly groups with 30 HT patients and 30 normotensive healthy volunteers, and two young groups with 30 HT patients and 30 normotensive healthy volunteers. Plasma total thiol (T-SH), advanced oxidation protein products (AOPPs), protein carbonyl (PCO), ischemia modified albumin (IMA), urine 8-hydroxy-2'-deoxyguanosine (8-OHdG), and prooxidant-antioxidant balance (PAB) levels were measured.


RESULTS
In the elderly HT group AOPPs, PCO, 8-OHdG, and PAB were significantly higher than the elderly control group. In the young HT group T-SH levels were significantly lower and the other oxidative stress parameters were significantly higher than the young control group. In the elderly control group AOPPs, PCO, IMA, 8-OHdG and PAB were significantly higher than the young control group. T-SH was significantly lower in the elderly control than the young control group. In the elderly HT group, T-SH levels were significantly lower and AOPPs, PCO, IMA, 8-OHdG, and PAB levels were significantly higher than the young HT group.


CONCLUSIONS
Protein and DNA cell damage occurs by oxidation of free radicals throughout life. Our study supports the view that these radicals may be responsible for the development of hypertension with aging process. Urine 8-OHdG levels can be used as a marker for oxidative DNA damage in the elderly hypertensive patients. Finally, our results suggest that oxidative stress may influence both the development and progression of hypertension and aging.
Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Serap
S
Yavuzer
Division of Geriatrics, Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Cerrahpasa, 34303, Istanbul, Turkey. drhakanyavuzer@gmail.com.
Hakan
H
Yavuzer
Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Mahir
M
Cengiz
Department of Biochemistry, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Hayriye
H
Erman
Division of Geriatrics, Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Cerrahpasa, 34303, Istanbul, Turkey.
Filiz
F
Demirdag
Division of Geriatrics, Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Cerrahpasa, 34303, Istanbul, Turkey.
Alper
A
Doventas
Central Research Laboratory, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Huriye
H
Balci
Division of Geriatrics, Department of Internal Medicine, Cerrahpasa Faculty of Medicine, Istanbul University, Cerrahpasa, 34303, Istanbul, Turkey.
Deniz Suna
DS
Erdincler
Department of Biochemistry, Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey.
Hafize
H
Uzun
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D004249:DNA Damage / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012709:Serum Albumin / Q000378:metabolism; D000075462:Serum Albumin, Human; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000975:Antioxidants; D015415:Biomarkers; D005609:Free Radicals; D012709:Serum Albumin; D013438:Sulfhydryl Compounds; C561522:ischemia-modified albumin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D000075462:Serum Albumin, Human
Aging; DNA damage; Hypertension; Oxidative stress; Protein oxidation