A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results.
Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin's expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin's receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis of GALR1 promoter can be a useful ancillary marker to cytology as the histological status can successfully predict. This marker has the potential to lead towards better management of women with endometrial lesions and eventually reduce unnecessary interventions. In addition it can provide early warning for women with negative cytological result.
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Christine
C
Kottaridi
2nd Department of Gynecology, St. Savas Cancer Hospital, 171 Alexandras Avenue, 11522 Athens, Greece.
Nikolaos
N
Koureas
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Niki
N
Margari
2nd Department of Gynecology, St. Savas Cancer Hospital, 171 Alexandras Avenue, 11522 Athens, Greece.
Emmanouil
E
Terzakis
Department of Obstetrics and Gynecology, IASO Hospital, 37-39 Kifisias Avenue, Maroussi, 15123 Athens, Greece.
Evripidis
E
Bilirakis
3rd Department of Obstetrics and Gynecology, "ATTIKON" University Hospital, 1 Rimini, Haidari, 12462 Athens, Greece.
Asimakis
A
Pappas
3rd Department of Obstetrics and Gynecology, "ATTIKON" University Hospital, 1 Rimini, Haidari, 12462 Athens, Greece.
Charalampos
C
Chrelias
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Aris
A
Spathis
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Evangelia
E
Aga
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Abraham
A
Pouliakis
2nd Department of Pathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Ioannis
I
Panayiotides
Department of Cytopathology, "ATTIKON" University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece.
Petros
P
Karakitsos
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D019175:DNA Methylation / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D004717:Endometrium / Q000737:chemistry*; D005260:Female; D006801:Humans; D008875:Middle Aged; D012372:ROC Curve; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA