Buckwheat Honey Attenuates Carbon Tetrachloride-Induced Liver and DNA Damage in Mice.
Buckwheat honey, which is widely consumed in China, has a characteristic dark color. The objective of this study was to investigate the protective effects of buckwheat honey on liver and DNA damage induced by carbon tetrachloride in mice. The results revealed that buckwheat honey had high total phenolic content, and rutin, hesperetin, and p-coumaric acid were the main phenolic compounds present. Buckwheat honey possesses super DPPH radical scavenging activity and strong ferric reducing antioxidant power. Administration of buckwheat honey for 10 weeks significantly inhibited serum lipoprotein oxidation and increased serum oxygen radical absorbance capacity. Moreover, buckwheat honey significantly inhibited aspartate aminotransferase and alanine aminotransferase activities, which are enhanced by carbon tetrachloride. Hepatic malondialdehyde decreased and hepatic antioxidant enzymes (superoxide dismutase and glutathione peroxidase) increased in the presence of buckwheat honey. In a comet assay, lymphocyte DNA damage induced by carbon tetrachloride was significantly inhibited by buckwheat honey. Therefore, buckwheat honey has a hepatoprotective effect and inhibits DNA damage, activities that are primarily attributable to its high antioxidant capacity.
Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China ; Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
Ni
N
Cheng
Institute of Apicultural Research, Chinese Academy of Agricultural Science, Beijing 100093, China.
Liming
L
Wu
Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
Jianbin
J
Zheng
Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China ; Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Institute of Analytical Science, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
Wei
W
Cao