Mechanisms of zinc toxicity in the galaxiid fish, Galaxias maculatus.
Zinc (Zn) is an essential metal, which is ubiquitous in aquatic environments occurring both naturally, and through anthropogenic inputs. This study investigated impacts of sub-lethal Zn exposure in the galaxiid fish Galaxias maculatus. Known as inanga, this amphidromous fish is widespread throughout the Southern hemisphere, but to date almost nothing is known regarding its sensitivity to elevated environmental metals. Fish were exposed to environmentally-relevant concentrations of Zn (control, 8, 270 and 1000μgL(-1)) over 96h. End-points measured included those relating to ionoregulatory disturbance (whole body calcium and sodium influx), oxygen consumption (respirometry), oxidative stress (catalase activity and lipid peroxidation) and whole body accumulation of Zn. Zn exposure caused increases in catalase activity and lipid peroxidation, but only at the highest exposure level tested. Zn also significantly inhibited calcium influx, but stimulated sodium influx, at 1000μgL(-1). The sub-lethal changes induced by Zn exposure in inanga appear to be conserved relative to other, better-studied species. These data are the first to explore the sensitivity of juvenile galaxiid fish to Zn, information that will be critical to ensuring adequate environmental protection of this important species.
University of Canterbury, Private Bag 4800, Christchurch, New Zealand. Electronic address: nicole.mcrae@pg.canterbury.ac.nz.
Nicole K
NK
McRae
University of Canterbury, Private Bag 4800, Christchurch, New Zealand. Electronic address: sally.gaw@canterbury.ac.nz.
Sally
S
Gaw
University of Canterbury, Private Bag 4800, Christchurch, New Zealand; Athabasca University, 1 University Drive, Athabasca, AB T9S 3A3, Canada. Electronic address: chris.glover@canterbury.ac.nz.
Chris N
CN
Glover
D000818:Animals; D004305:Dose-Response Relationship, Drug; D000075322:Heavy Metal Poisoning; D019216:Metals, Heavy / Q000378:metabolism; D036441:Osmeriformes; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D011041:Poisoning / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015032:Zinc / Q000633:toxicity*
D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D015032:Zinc
Calcium; Galaxiid; Ion regulation; Lipid peroxidation; Oxidative stress toxicity; Sodium; Zinc