Spatial distribution of metabolites in the human lens.
Spatial distribution of 34 metabolites along the optical and equatorial axes of the human lens has been determined. For the majority of metabolites, the homogeneous distribution has been observed. That suggests that the rate of the metabolite transformation in the lens is low due to the general metabolic passivity of the lens fiber cells. However, the redox processes are active in the lens; as a result, some metabolites, including antioxidants, demonstrate the "nucleus-depleted" type of distribution, whereas secondary UV filters show the "nucleus-enriched" type. The metabolite concentrations at the lens poles and equator are similar for all metabolites under study. The concentric pattern of the "nucleus-depleted" and "nucleus-enriched" distributions testifies that the metabolite distribution inside the lens is mostly governed by a passive diffusion, relatively free along the fiber cells and retarded in the radial direction across the cells. No significant difference in the metabolite distribution between the normal and cataractous human lenses was found.
International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk, 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia.
Semen O
SO
Tamara
International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk, 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia.
Lyudmila V
LV
Yanshole
International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk, 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia.
Vadim V
VV
Yanshole
Novosibirsk State Regional Clinical Hospital, Nemirovicha-Danchenko 130, Novosibirsk, 630087, Russia.
Anjella Zh
AZh
Fursova
Novosibirsk Regional Clinical Bureau of Forensic Medical Examination, Nemirovicha-Danchenko 134, Novosibirsk, 630087, Russia.
Denis A
DA
Stepakov
Novosibirsk Regional Clinical Bureau of Forensic Medical Examination, Nemirovicha-Danchenko 134, Novosibirsk, 630087, Russia.
Vladimir P
VP
Novoselov
International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk, 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia. Electronic address: yura@tomo.nsc.ru.
Yuri P
YP
Tsentalovich
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology; D002386:Cataract / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D013058:Mass Spectrometry; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics / Q000379:methods; D008875:Middle Aged; D014019:Tissue Donors
D005136:Eye Proteins
Antioxidants; Cataract; Lens; Metabolites; Spatial distribution