Functional fluxolipidomics of polyunsaturated fatty acids and oxygenated metabolites in the blood vessel compartment.
Synthesis of bioactive oxygenated metabolites of polyunsaturated fatty acids and their degradation or transformation products are made through multiple enzyme processes. The kinetics of the enzymes responsible for the different steps are known to be quite diverse, although not precisely determined. The location of the metabolites biosynthesis is diverse as well. Also, the biological effects of the primary and secondary products, and their biological life span are often completely different. Consequently, phenotypes of cells in response to these bioactive lipid mediators must then depend on their concentrations at a given time. This demands a fluxolipidomics approach that can be defined as a mediator lipidomics, with all measurements done as a function of time and biological compartments. This review points out what is known, even qualitatively, in the blood vascular compartment for arachidonic acid metabolites and number of other metabolites from polyunsaturated fatty acids of nutritional value. The functional consequences are especially taken into consideration.
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France. Electronic address: michel.lagarde@insa-lyon.fr.
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Lagarde
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Calzada
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Jouvène
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Bernoud-Hubac
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Létisse
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Guichardant
Université de Lyon, Inserm UMR 1060, Inra UMR 1397, IMBL, INSA-Lyon, Villeurbanne, France.
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Véricel
D001792:Blood Platelets / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000097:blood* / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D064688:Metabolic Flux Analysis; D010100:Oxygen / Q000097:blood / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D010100:Oxygen