Monitoring photodynamic therapy with photoacoustic microscopy.
Abstract. We present our work on examining the feasibility of monitoring photodynamic therapy (PDT)-induced vasculature change with acoustic-resolution photoacoustic microscopy (PAM). Verteporfin, an FDA-approved photosensitizer for clinical PDT, was utilized. With a 60-μm-resolution PAM system, we demonstrated the capability of PAM to monitor PDT-induced vasculature variations in a chick chorioallantoic membrane model with topical application and in a rat ear with intravenous injection of the photosensitizer. We also showed oxygen saturation change in target blood vessels due to PDT. Success of the present approach may potentially lead to the application of PAM imaging in evaluating PDT efficacy, guiding treatment, and predicting responders from nonresponders.
University of Alberta, Department of Electrical & Computer Engineering, 9107-116 Street, Edmonton T6G 2V4, Canada.
Peng
P
Shao
University of Alberta, Department of Surgery and Oncology, 11560 University Avenue, Edmonton T6G 1Z2, Canada.
David W
DW
Chapman
University of Alberta, Department of Surgery and Oncology, 11560 University Avenue, Edmonton T6G 1Z2, Canada.
Ronald B
RB
Moore
University of Alberta, Department of Electrical & Computer Engineering, 9107-116 Street, Edmonton T6G 2V4, Canada.
Roger J
RJ
Zemp
D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005240:Feasibility Studies; D033401:Microscopy, Acoustic / Q000295:instrumentation / Q000379:methods*; D061088:Photoacoustic Techniques / Q000295:instrumentation / Q000379:methods*; D010778:Photochemotherapy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome