Production of Ginsenoside F2 by Using Lactococcus lactis with Enhanced Expression of β-Glucosidase Gene from Paenibacillus mucilaginosus.
This study aimed to produce a pharmacologically active minor ginsenoside F2 from the major ginsenosides Rb1 and Rd by using a recombinant Lactococcus lactis strain expressing a heterologous β-glucosidase gene. The nucleotide sequence of the gene (BglPm) was derived from Paenibacillus mucilaginosus and synthesized after codon optimization, and the two genes (unoptimized and optimized) were expressed in L. lactis NZ9000. Codon optimization resulted in reduction of unfavorable codons by 50% and a considerable increase in the expression levels (total activities) of β-glucosidases (0.002 unit/mL, unoptimized; 0.022 unit/mL, optimized). The molecular weight of the enzyme was 52 kDa, and the purified forms of the enzymes could successfully convert Rb1 and Rd into F2. The permeabilized L. lactis expressing BglPm resulted in a high conversion yield (74%) of F2 from the ginseng extract. Utilization of this microbial cell to produce F2 may provide an alternative method to increase the health benefits of Panax ginseng.
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University , Cheongju 361-763, Korea.
Ling
L
Li
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University , Cheongju 361-763, Korea.
So-Yeon
SY
Shin
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University , Cheongju 361-763, Korea.
Soo Jin
SJ
Lee
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University , Cheongju 361-763, Korea.
Jin Seok
JS
Moon
Department of Biotechnology, Hankyong National University , Kyonggi-do 456-749, Korea.
Wan Taek
WT
Im
Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural and Food Sciences, Chungbuk National University , Cheongju 361-763, Korea.
Nam Soo
NS
Han
D001407:Bacillus / Q000378:metabolism; D036145:Ginsenosides / Q000032:analysis*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D008970:Molecular Weight; D056507:Paenibacillus / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D005894:Panax / Q000378:metabolism; D014992:Xylenes / Q000737:chemistry; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
D036145:Ginsenosides; D014992:Xylenes; C407883:ginsenoside F2; C442759:ginsenoside Rb1; D001617:beta-Glucosidase
Lactococcus lactis; ginsenoside F2; β-glucosidase