Extraction, characterization and gelling behavior enhancement of pectins from the cladodes of Opuntia ficus indica.
Total Pectins Fraction (TPF) was extracted at room temperature from dried cladodes of Opuntia ficus indica. TPF is constituted of three pectic fractions WSP, CSP and ASP, which are made up of 66.6%, 44.3% and 81.1% (w/w) of galacturonic acid, respectively. The antioxidant ability of TPF increased with the concentration increasing. It scavenged hydroxyl radical by 90% and chelated 90% of ferrous ions at 5 g/L. FTIR study was carried out. Strong characteristic absorption peaks at 1,618 cm(-1) assigned to the vibration of COO(-) group of galacturonic acid. In the fingerprint region, we noticed three well-defined peaks at 1054, 1085, and 1,154 cm(-1) characteristic of pectic polysaccharides. TPF are non-gelling pectins. The co-crosslinking of TPF with carrageenan was carried out and the gelling behavior was successfully improved. Thermo-sensitive hydrogel was obtained with 82% of TPF and 18% of carrageenan (w/w).
Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria; Département de Biochimie et Microbiologie, Faculté des Sciences Biologiques et des Sciences Agronomiques, Université de Tizi ouzou, 15000 Tizi ouzou, Algeria. Electronic address: klefsih@yahoo.fr.
Khalef
K
Lefsih
Clermont Université, Université Blaise Pascal, Institut Pascal, UMR CNRS 6602 CNRS Polytech Clermont-Ferrand, 24 avenue des Landais, BP 206, Aubière Cedex F-63174, France.
Cédric
C
Delattre
Clermont Université, Université Blaise Pascal, Institut Pascal, UMR CNRS 6602 CNRS Polytech Clermont-Ferrand, 24 avenue des Landais, BP 206, Aubière Cedex F-63174, France.
Guillaume
G
Pierre
Clermont Université, Université Blaise Pascal, Institut Pascal, UMR CNRS 6602 CNRS Polytech Clermont-Ferrand, 24 avenue des Landais, BP 206, Aubière Cedex F-63174, France.
Philippe
P
Michaud
Department of Pharmaceutical Engineering and Chemistry, Soniya College of Pharmacy, Dharwad 580 002, India.
Tejraj M
TM
Aminabhavi
Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
Farid
F
Dahmoune
Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
Khodir
K
Madani
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002118:Calcium / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D005782:Gels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000737:chemistry; D029747:Opuntia / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014783:Viscosity
D000975:Antioxidants; D002614:Chelating Agents; D016166:Free Radical Scavengers; D005782:Gels; D009005:Monosaccharides; D010936:Plant Extracts; D010368:Pectins; D002118:Calcium
Antioxidant; Carrageenan; Co-crosslinking; FTIR; Gelling behavior; Opuntia ficus indica; Pectin; Viscosity