Transcriptome analysis of Rpl11-deficient zebrafish model of Diamond-Blackfan Anemia.
To comprehensively reflect the roles of Rpl11 on the transcriptome of zebrafish model of Diamond-Blackfan Anemia (DBA), we performed whole-genome transcriptome sequencing on the Illumina Hi-Seq 2000 sequencing platform. Two different transcriptomes of zebrafish Rpl11-deficient and control Morpholino (Mo) embryos were collected and analyzed. The experimental design and methods, including sample preparation, RNA-Seq data evaluation and treatment, were described in details so that representative high-throughput sequencing data were acquired for assessing the actual impacts of Rpl11 on zebrafish embryos. We provided the accession number GSE51326 for easy access to the database.
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Zhaojun
Z
Zhang
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Haibo
H
Jia
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Qian
Q
Zhang
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Yang
Y
Wan
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Binfeng
B
Song
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Qiong
Q
Jia
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Hanzhi
H
Liu
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Xiaofan
X
Zhu
CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Xiangdong
X
Fang
DBA; High-Seq 2000; IPA; Transcriptome; Zebrafish