Formation of Tethers from Spreading Cellular Aggregates.
Membrane tubes are commonly extruded from cells and vesicles when a point-like force is applied on the membrane. We report here the unexpected formation of membrane tubes from lymph node cancer prostate (LNCaP) cell aggregates in the absence of external applied forces. The spreading of LNCaP aggregates deposited on adhesive glass substrates coated with fibronectin is very limited because cell-cell adhesion is stronger than cell-substrate adhesion. Some cells on the aggregate periphery are very motile and try to escape from the aggregate, leading to the formation of membrane tubes. Tethered networks and exchange of cargos between cells were observed as well. Growth of the tubes is followed by either tube retraction or tube rupture. Hence, even very cohesive cells are successful in escaping aggregates, which may lead to epithelial mesenchymal transition and tumor metastasis. We interpret the dynamics of formation and retraction of tubes in the framework of membrane mechanics.
WPI International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) , 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Grégory
G
Beaune
WPI International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS) , 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.|Department of Chemistry and Faculty of Pharmacy, University of Montreal , CP 6128 Succursale Centre Ville, Montreal, QC H3C3J7, Canada.
Françoise M
FM
Winnik
Institut Curie , PSL Research University, CNRS, UMR 168, F-75005 Paris, France.|Sorbonne Universitiés , UPMC Univ Paris 06, CNRS, UMR 168, F-75005 Paris, France.
Françoise
F
Brochard-Wyart
D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D005353:Fibronectins / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D008297:Male
D005353:Fibronectins