Effects of dimethoate in male mice reproductive parameters.
The aim of the current study was to investigate the ability of dimethoate (DMT) to induce reprotoxicity in male mice. The dose (20 mg/kg/day) was given orally for 30 days. A significant decrease in sperm count, motility and viability and a significant increase of morphologically abnormal spermatozoa percent in DMT treated mice was observed. Testicular Acetylcholinesterase (AChE) and Butyrylcholinesterase (BChE) activities were inhibited. Also, a significant increase in lipid peroxidation level and a significant decrease in the activities of antioxidant enzymes were observed in testis of DMT mice. In addition, gene expression of glutathione peroxidase 4 (GPx4) was quantified in RNA samples extracted from the testis by real-time reverse transcription-polymerase chain reaction (RT-PCR). Compared with control, mRNA expression of GPx4 was slightly decreased after DMT-exposure.
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia. Electronic address: Jallouli-manel@hotmail.fr.
Manel
M
Jallouli
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
Ines El Bini
Iel B
Dhouib
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
Hanène
H
Dhouib
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
Najoua
N
Gharbi
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
Saloua
S
El Fazaa
D000110:Acetylcholinesterase / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004117:Dimethoate / Q000008:administration & dosage / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012098:Reproduction / Q000187:drug effects*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
D000975:Antioxidants; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D004117:Dimethoate
Dimethoate; Mice; Oxidative stress; Reprotoxicity; Testis