Chemical Signals of Synthetic Disaccharide Derivatives Dominate Rhamnolipids at Controlling Multiple Bacterial Activities.
Microbes secrete molecules that modify their environment. Here, we demonstrate a class of synthetic disaccharide derivatives (DSDs) that mimics and dominates the activity of naturally secreted rhamnolipids by Pseudomonas aeruginosa. The DSDs exhibit the dual function of activating and inhibiting the swarming motility through a concentration-dependent activity reversal that is characteristic of signaling molecules. Whereas DSDs tethered with a saturated farnesyl group exhibit inhibition of both biofilm formation and swarming motility, with higher activities than rhamnolipids, a saturated farnesyl tethered with a sulfonate group only inhibits swarming motility but promote biofilm formation. These results identified important structural elements for controlling swarming motility, biofilm formation, and bacterial adhesion and suggest an effective chemical approach to control intertwined signaling processes that are important for biofilm formation and motilities.
Department of Chemistry, Syracuse University, 1-014 CST, 111 College Place, Syracuse, NY, 13244, USA.
Nischal
N
Singh
Department of Chemistry, Syracuse University, 1-014 CST, 111 College Place, Syracuse, NY, 13244, USA.
Gauri S
GS
Shetye
Department of Chemistry, Syracuse University, 1-014 CST, 111 College Place, Syracuse, NY, 13244, USA.
Hewen
H
Zheng
Department of Chemistry, Syracuse University, 1-014 CST, 111 College Place, Syracuse, NY, 13244, USA.
Jiayue
J
Sun
Department of Chemistry, Syracuse University, 1-014 CST, 111 College Place, Syracuse, NY, 13244, USA. yluk@syr.edu.
Yan-Yeung
YY
Luk
D018441:Biofilms / Q000187:drug effects; D004187:Disaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D006017:Glycolipids / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology*
D004187:Disaccharides; D006017:Glycolipids; C418382:rhamnolipid
Pseudomonas aeruginosa; biofilms; rhamnolipids; swarming