Mono-(2-ethylhexyl) phthalate (MEHP) affects intercellular junctions of Sertoli cell: A potential role of oxidative stress.
We analyzed the potential role of oxidative stress induced by mono (2-ethylhexyl) phthalate (MEHP) in adherent cell junction protein expression of prepubertal rat Sertoli cells (SC) in vitro. Five-day SC cultures were treated with MEHP (200μM) for 24h and compared to cells in basal conditions. Western blot and immunofluorescent (IF) analyses showed that MEHP induced increase of N-cadherin and catenin expression, modifying its distribution. Concomitantly, Cx-43 expression decreased significantly and delocalization of the IF signal for tight junction proteins (occludin, claudin-11 and ZO-1) occurred. Indicative of oxidative stress, MEHP induced in SC an increase of lipoperoxides, a decrease in glutathione (GSH) levels and a concomitant increase in Glutathione S-Transferases (GST) activity. Antioxidant N-acetyl-cysteine (1mM) treatment prevented GSH decrease and N-cadherin and α-catenin up-regulation induced by MEHP. Our data suggest that oxidative stress signaling is a mechanism involved in adherent cell junctions disruption induced by MEHP in SC cultures.
INBIOMED-UBA-CONICET, Facultad de Medicina UBA, Buenos Aires, Argentina. Electronic address: ciruba02@hotmail.com.ar.
Cristian M
CM
Sobarzo
Centro de Investigaciones Endocrinológicas (CEDIE), Dr. C. Bergadá. CONICET - FEI - División de Endocrinología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina; Departamento de Fisiologia, Universidade Federal do Paraná Curitiba, PR, Brazil.
Nogueira de Morais
Nde M
Rosana
INBIOMED-UBA-CONICET, Facultad de Medicina UBA, Buenos Aires, Argentina.
Lustig
L
Livia
INBIOMED-UBA-CONICET, Facultad de Medicina UBA, Buenos Aires, Argentina.
Denduchis
D
Berta
Centro de Investigaciones Endocrinológicas (CEDIE), Dr. C. Bergadá. CONICET - FEI - División de Endocrinología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina.
Helena F
HF
Schteingart
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D062445:Claudin-1 / Q000378:metabolism; D018031:Connexin 43 / Q000378:metabolism; D019610:Cytoprotection; D004051:Diethylhexyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007365:Intercellular Junctions / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D062793:Occludin / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D062826:Zonula Occludens-1 Protein / Q000378:metabolism; D051178:alpha Catenin / Q000378:metabolism
D000975:Antioxidants; D015820:Cadherins; D062445:Claudin-1; C568114:Cldn1 protein, rat; D018031:Connexin 43; C580592:Gja1 protein, rat; C570099:N-cadherin, rat; D009419:Nerve Tissue Proteins; D062793:Occludin; C568987:Ocln protein, rat; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D051178:alpha Catenin; D004051:Diethylhexyl Phthalate; D005982:Glutathione Transferase; C016599:mono-(2-ethylhexyl)phthalate; D005978:Glutathione
Adherent junction proteins; Glutathione; MEHP; Oxidative stress; Sertoli cell