An investigation and evaluation on species and characteristics of pathogenic microorganisms in Chinese local hospital settings.
There are currently great concerns about the level of bacterial contamination in hospitals, as well as resistance to antimicrobial agents. The species and characteristics of microbes in Chinese hospitals are closely related to healthcare safety and the prevention and control of infections. However, data on the exposure of patients to microbes in Chinese hospitals are limited. The present study investigated the genera of microorganisms in Chinese hospitals. We evaluated their characteristics, such as antibiotic susceptibility, tolerance to disinfectants, and toxicity, using silkworms (Bombyx mori) as an animal model. Twenty-six distinct bacterial strains were isolated, and their genera were determined by sequencing their 16S rDNA regions. Twenty-five strains were resistant to one or more antibiotics, and six strains were resistant to multiple antibiotics. The results of minimal inhibitory concentration testing showed that eight strains were resistant to a chlorine-containing disinfectant, and 12 strains were resistant to Povidone-iodine. Following the injection of bacterial cultures into the silkworm hemolymph, 15 strains killed all of the silkworms within 5 d. Additionally, bacterial strain 14 killed all of silkworms within 12 h with a median lethal dose of 4 × 10(4) colony-forming units/larva. This study provides useful information for healthcare safety in Chinese hospitals.
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Liang
L
Chen
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China; Zhejiang Institute for the Control of Medical Device, Hangzhou, Zhejiang, China.
Xiaoli
X
Lü
The Fourth People's Hospital of Zhenjiang, Jiangsu, China.
Weiping
W
Cao
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Chunxia
C
Zhang
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Rongfa
R
Xu
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Xu
X
Meng
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China. Electronic address: kpchen@ujs.edu.cn.
Keping
K
Chen
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D001424:Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D012831:Bombyx / Q000502:physiology; D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004202:Disinfectants / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D006761:Hospitals; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D014774:Virulence
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004202:Disinfectants; D012336:RNA, Ribosomal, 16S
Antimicrobial resistance; Hospital settings; Microorganisms; Toxicity