Expression of Endometrial Receptivity Genes Increase After Myomectomy of Intramural Leiomyomas not Distorting the Endometrial Cavity.
This study was designed to investigate whether endometrial receptivity genes are altered in infertile patients with intramural leiomyomas (IM) not distorting the endometrial cavity undergoing myomectomy. We measured endometrial HOXA-10, HOXA-11, LIF, ITGB3, and ITGAV messenger RNA (mRNA) expressions levels before and after myomectomy/metroplasty during mid-luteal phase in participants with IM, submucosal leiomyomas (SM), and septate uterus and fertile participants without fibroids. Initial endometrial sampling was obtained at the time of surgery, and second sampling was obtained 3 months after myomectomy/metroplasty. Expressions of each gene were evaluated using real-time reverse transcriptase polymerase chain reaction (RT-PCR). A trend toward decreased endometrial HOXA-10, HOXA-11, and ITGAV mRNA expression was detected in both SM and IM groups before myomectomy when compared to both fertile group and septate uterus. However, the differences failed to show statistical significance. After myomectomy of IM, we have detected 12.8-fold increase in endometrial HOXA-10 mRNA expression and 9.0-fold increase in endometrial HOXA-11 mRNA expression. This increase in endometrial HOXA-10 and 11 mRNA expression was significant. Accordingly, 2 patients having intramural fibroids greater than 5 cm were able to remain pregnant after myomectomy. Conversely, submucosal myomectomy did not cause any significant effect on endometrial receptivity markers. Likewise, all markers of endometrial receptivity remained unchanged after metroplasty. Myomectomy of IM have favorable effect on endometrial HOXA-10 and 11 mRNA expression.
Department of Obstetrics and Gynecology, School of Medicine, Acıbadem University, Istanbul, Turkey.
Cihat
C
Unlu
Department of Obstetrics and Gynecology, Usak, Turkey celiksudenaz@gmail.com mcihatunlu@gmail.com.
Onder
O
Celik
Department of Biochemistry, Behçet Uz Children's Hospital, İzmir, Turkey.
Nilufer
N
Celik
Department of Medical Microbiology, School of Medicine, Inonu University, Malatya, Turkey.
Baris
B
Otlu
D000328:Adult; D016022:Case-Control Studies; D004717:Endometrium / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005786:Gene Expression Regulation; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007247:Infertility, Female / Q000235:genetics / Q000378:metabolism / Q000601:surgery; D039661:Integrin beta3 / Q000235:genetics / Q000378:metabolism; D007889:Leiomyoma / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D053605:Leukemia Inhibitory Factor / Q000235:genetics / Q000378:metabolism; D063186:Uterine Myomectomy; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000601:surgery
C484298:HOXA11 protein, human; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; C061248:ITGB3 protein, human; D039661:Integrin beta3; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C092119:Hoxa10 protein, mouse
endometrial receptivity genes; implantation; intramural leiomyomas; myomectomy; septate uterus; submucosal leiomyomas