Micromonospora lycii sp. nov., a novel endophytic actinomycete isolated from wolfberry root (Lycium chinense Mill).
A novel actinomycete, designated strain NEAU-gq11(T), was isolated from wolfberry root (Lycium chinense Mill) and characterized using a polyphasic approach. Strain NEAU-gq11(T) was observed to form rough-surfaced spores that borne singly on the substrate hyphae but aerial mycelia were not developed. The organism showed closest 16S rRNA gene sequence similarity to Micromonospora saelicesensis Lupac 09(T) (99.4%), and phylogenetically clustered with M. chokoriensis 2-19/6(T) (99.3%), 'M. zeae' NEAU-gq9(T) (99.3%), M. violae NEAU-zh8(T) (99.1%), 'M. jinlongensis' NEAU-GRX11(T) (99.1%), M. zamorensis CR38(T) (99.0%), M. taraxaci NEAU-P5(T) (98.9%) and M. lupini Lupac 14N(T) (98.6%). Phylogenetic analysis based on the gyrB gene sequences also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-10(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acid was iso-C16:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-gq11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora lycii sp. nov. is proposed. The type strain is NEAU-gq11(T) (=CGMCC 4.7149(T)=DSM 46703(T)).
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Junwei
J
Zhao
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Lifeng
L
Guo
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Chongxi
C
Liu
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Yuejing
Y
Zhang
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Xuejiao
X
Guan
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Jiansong
J
Li
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Shilin
S
Xu
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.|State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Wensheng
W
Xiang
School of Life Science, Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, PR China.
Xiangjing
X
Wang
D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D005998:Glycine / Q000737:chemistry; D032305:Lycium / Q000382:microbiology*; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D005998:Glycine