Paenibacillus terreus sp. nov., isolated from forest soil.
A Gram-stain-positive, rod-shaped, endospore-forming, motile bacterium, designated D33T, was isolated from a forest soil sample. The strain grew optimally at 30-37 °C, pH 8.0 and with 1 % (w/v) NaCl. The 16S rRNA gene sequence of the isolate showed similarities lower than 97 % with respect to species of the genus Paenibacillus. Strain D33T contained meso-diaminopimelic acid in the cell-wall peptidoglycan, and ribose and lower amounts of glucose and galactose as the whole-cell sugars. The major cellular fatty acid was anteiso-C15 : 0, and menaquinone-7 (MK-7) was the only respiratory quinone. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, two glycolipids and an unknown lipid. The DNA G+C content was 51.1 mol%. The low DNA-DNA relatedness values between strain D33T and recognized species of the genus Paenibacillus, together with many phenotypic properties supported the classification of strain D33T as representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus terreus sp. nov. is proposed. The type strain is D33T ( = KACC 18491T = DSM 100035T = CCTCC AB 2015273T).
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Zhi
Z
Huang
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Wenjuan
W
Dai
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Zhijun
Z
Zhou
Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Guoxiang
G
Wang
Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Guoqing
G
Lin
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Xixue
X
Yan
State Key Laboratory Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, PR China.
Fei
F
Zhao
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D065928:Forests; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7