Immobilization of inorganic pyrophosphatase on nanodiamond particles retaining its high enzymatic activity.
Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsorption of PPase such as ND size and surface chemistry were studied. The obtained material is a submicron size association of ND particles and protein molecules in approximately equal amounts. Both covalently and noncovalently immobilized PPase retains a significant enzymatic activity (up to 95% of its soluble form) as well as thermostability. The obtained hybrid material has a very high enzyme loading capacity (∼1 mg mg(-1)) and may be considered as a promising delivery system of biologically active proteinaceous substances, particularly in the treatment of diseases such as calcium pyrophosphate crystal deposition disease and related pathologies. They can also be used as recoverable heterogeneous catalysts in the traditional uses of PPase.
Chemistry Department, Lomonosov Moscow State University, 1, Build. 3, Leninskie Gory St., Moscow 119992, Russia.
Elena V
EV
Rodina
Pharmaceutical Department, Pavlov Ryazan State Medical University, 9 Vysokovol'tnaya St., Ryazan 390026, Russia.
Anastasiya V
AV
Valueva
Pharmaceutical Department, Pavlov Ryazan State Medical University, 9 Vysokovol'tnaya St., Ryazan 390026, Russia.
Ruslan Yu
RY
Yakovlev
Chemistry Department, Lomonosov Moscow State University, 1, Build. 3 Leninskie Gory St., Moscow 119992, Russia.
Nataliya N
NN
Vorobyeva
Chemistry Department, Lomonosov Moscow State University, 1, Build. 3 Leninskie Gory St., Moscow 119992, Russia.
Inna I
II
Kulakova
Chemistry Department, Lomonosov Moscow State University, 1, Build. 3 Leninskie Gory St., Moscow 119992, Russia.
Georgy V
GV
Lisichkin
Pharmaceutical Department, Pavlov Ryazan State Medical University, 9 Vysokovol'tnaya St., Ryazan 390026, Russia.
Nikolay B
NB
Leonidov
D000327:Adsorption; D055598:Chemical Phenomena; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D043564:Inorganic Pyrophosphatase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D058612:Nanodiamonds / Q000737:chemistry*; D011485:Protein Binding; D013696:Temperature
D004800:Enzymes, Immobilized; D058612:Nanodiamonds; D043564:Inorganic Pyrophosphatase