Development and characterization of 25 microsatellite primers for Ilex chinensis (Aquifoliaceae).
OBJECTIVE
To evaluate genetic variation and structure of Ilex chinensis (Aquifoliaceae), a dioecious evergreen tree, we developed 25 microsatellite markers from its nuclear genome.


RESULTS
Based on the biotin-streptavidin capture method, 10 polymorphic and 15 monomorphic microsatellite markers were developed. Ten polymorphic loci were characterized by 87 individuals sampled from three populations located in Zhejiang Province and Shanghai, China. The number of alleles per locus varied from two to 12. The observed and expected heterozygosities were 0.0435-0.9032 and 0.3121-0.8343, respectively.


CONCLUSIONS
These microsatellite markers can be useful for further genetic studies of I. chinensis populations, and so contribute to forest restoration and management.
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Wen-Wen
WW
Chen
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Zhen-Zhu
ZZ
Xiao
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Xin
X
Tong
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Yu-Pei
YP
Liu
School of Ecological and Environmental Sciences, Tiantong National Station of Forest Ecosystem, Shanghai Key Laboratory for Urban Ecology and Restoration, East China Normal University, Shanghai 200241, People's Republic of China.
Yuan-Yuan
YY
Li
Aquifoliaceae; Ilex chinensis; evergreen broadleaved forests; genetic diversity; genetic structure; simple sequence repeat (SSR)