Structural Characterization and Antimicrobial Activity of a Biosurfactant Obtained From Bacillus pumilus DSVP18 Grown on Potato Peels.
BACKGROUND
Biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. They are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. Major limiting factor in biosurfactant production is their production cost.


OBJECTIVE
The aim of this study was to investigate biosurfactant production under laboratory conditions with potato peels as the sole source of carbon source.


METHODS
A biosurfactant-producing bacterial strain (Bacillus pumilus DSVP18, NCBI GenBank accession no. GQ865643) was isolated from motor oil contaminated soil samples. Biochemical characteristics of the purified biosurfactant were determined and its chemical structure was analyzed. Stability studies were performed and biological activity of the biosurfactant was also evaluated.


RESULTS
The strain, when grown on modified minimal salt media supplemented with 2% potato peels as the sole carbon source, showed the ability to reduce Surface Tension (ST) value of the medium from 72 to 28.7 mN/m. The isolated biosurfactant (3.2 ± 0.32 g/L) was stable over a wide range of temperatures (20 - 120 ºC), pH (2-12) and salt concentrations (2 - 12%). When characterized using high-performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy, it was found to be a lipopeptide in nature, which was further confirmed by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (mass peak 1044.60) and nuclear magnetic resonance (NMR) studies. Data showed that the isolated biosurfactant at the concentration range of 30 - 35 µg/ml had strong antimicrobial activity when tested against standard strains of Bacillus cereus, Escherichia coli, Salmonella enteritidis, Staphylococcus aureus and Paenibacillus larvae.


CONCLUSIONS
Potato peels were proved to be potentially useful substrates for biosurfactant production by B. pumilus DSVP18. The strain possessed a unique property to reduce surface tension of the media from 72 to 28.7 mN/m. In addition, it showed a stable surface activity over a wide range of temperatures, pH, and saline conditions and had strong antimicrobial activity. This potential of the identified biosurfactant can be exploited by pharmaceutical industries for its commercial usage.
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Deepak
D
Sharma
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India ; Bee Research Chair, Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, KSA.
Mohammad Javed
MJ
Ansari
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Sonam
S
Gupta
Bee Research Chair, Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, KSA.
Ahmad
A
Al Ghamdi
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Parul
P
Pruthi
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Vikas
V
Pruthi
Anti-Microbial Agents; Lipopeptides; Paenibacillus; Surface Active Agents; Surface Tension