Anvi'o: an advanced analysis and visualization platform for 'omics data.
Advances in high-throughput sequencing and 'omics technologies are revolutionizing studies of naturally occurring microbial communities. Comprehensive investigations of microbial lifestyles require the ability to interactively organize and visualize genetic information and to incorporate subtle differences that enable greater resolution of complex data. Here we introduce anvi'o, an advanced analysis and visualization platform that offers automated and human-guided characterization of microbial genomes in metagenomic assemblies, with interactive interfaces that can link 'omics data from multiple sources into a single, intuitive display. Its extensible visualization approach distills multiple dimensions of information about each contig, offering a dynamic and unified work environment for data exploration, manipulation, and reporting. Using anvi'o, we re-analyzed publicly available datasets and explored temporal genomic changes within naturally occurring microbial populations through de novo characterization of single nucleotide variations, and linked cultivar and single-cell genomes with metagenomic and metatranscriptomic data. Anvi'o is an open-source platform that empowers researchers without extensive bioinformatics skills to perform and communicate in-depth analyses on large 'omics datasets.
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States ; Department of Medicine, The University of Chicago , Chicago, IL , United States.
A Murat
AM
Eren
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States.
Özcan C
ÖC
Esen
Warwick Medical School, University of Warwick , Coventry , United Kingdom.
Christopher
C
Quince
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States.
Joseph H
JH
Vineis
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States.
Hilary G
HG
Morrison
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States.
Mitchell L
ML
Sogin
Josephine Bay Paul Center, Marine Biological Laboratory , Woods Hole, MA , United States.
Tom O
TO
Delmont
Assembly; Genome binning; Metagenomics; Metatranscriptomics; SNP profiling; Visualization