High-performance liquid chromatographic separations of stereoisomers of chiral basic agrochemicals with polysaccharide-based chiral columns and polar organic mobile phases.
The separation of the stereoisomers of 23 chiral basic agrochemicals was studied on six different polysaccharide-based chiral columns in high-performance liquid chromatography with various polar organic mobile phases. Along with the successful separation of analyte stereoisomers, emphasis was placed on the effect of the chiral selector and mobile phase composition on the elution order of stereoisomers. The interesting phenomenon of reversal of enantiomer/stereoisomer elution order function of the polysaccharide backbone (cellulose or amylose), type of derivative (carbamate or benzoate), nature, and position of the substituent(s) in the phenylcarbamate moiety (methyl or chloro) and the nature of the mobile phase was observed. For several of the analytes containing two chiral centers all four stereoisomers were resolved with at least one chiral selector/mobile phase combination.
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Iza
I
Matarashvili
Department of Ecology and Environmental Protection, Faculty of Informatics, Mathematics and Natural Sciences, Georgian University of St. Andrew the Apostle, Tbilisi, Georgia.
Iamze
I
Shvangiradze
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Lali
L
Chankvetadze
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.|Department of Ecology and Environmental Protection, Faculty of Informatics, Mathematics and Natural Sciences, Georgian University of St. Andrew the Apostle, Tbilisi, Georgia.
Shota
S
Sidamonidze
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Nino
N
Takaishvili
Phenomenex Inc, Torrance, CA, USA.
Tivadar
T
Farkas
Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
Bezhan
B
Chankvetadze
Chiral agrochemicals; Enantiomer elution order; High-performance liquid chromatography; Stereoisomer elution order