Evaluation of a novel technique for intraperitoneal injections in mice.
Intraperitoneal injection is a common technique that safely delivers a substance into the peritoneal cavity but can induce high stress in animals. The authors have developed a new method for administering intraperitoneal injections in mice, with the goal of causing less stress during handling and injection. Here, they compare their novel technique with a conventional technique in three experiments. In the first experiment, the authors administered intraperitoneal injections of contrast medium using either technique and then used micro-computed tomography to evaluate the placement and retention of the medium. In the second and third experiments, the authors administered intraperitoneal injections or control treatments, then sampled blood to determine circulating concentrations of stress-related hormones. Imaging showed that both the novel and the conventional techniques properly delivered a contrast medium into the peritoneal cavity. The novel technique was also associated with lower concentrations of stress-related hormones than was the conventional technique. These results indicate that this novel technique might be beneficial to investigators that use intraperitoneal injections with mice.
Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk, Korea.
Jong Min
JM
Baek
Korea Institute of Science and Technology for Eastern Medicine, NeuMed Inc., Seoul, Korea.
Sung Chul
SC
Kwak
Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.
Ju-Young
JY
Kim
Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk, Korea.
Sung-Jun
SJ
Ahn
Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.
Hong Young
HY
Jun
Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.|Department of Radiology, School of Medicine, Wonkwang University, Iksan, Jeonbuk, Korea.
Kwon-Ha
KH
Yoon
Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.|Division of Rheumatology, Department of Internal Medicine, Wonkwang University, Iksan, Jeonbuk, Korea.|Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk, Korea.
Myeng Su
MS
Lee
Department of Anatomy, School of Medicine, Wonkwang University, Iksan, Jeonbuk, Korea.|Imaging Science-Based Lung and Bone Diseases Research Center, Wonkwang University, Iksan, Jeonbuk, Korea.|Institute for Skeletal Disease, Wonkwang University, Iksan, Jeonbuk, Korea.
Jaemin
J
Oh
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007274:Injections, Intraperitoneal / Q000379:methods / Q000662:veterinary*; D008297:Male; D051379:Mice / Q000097:blood; D008813:Mice, Inbred ICR; D013315:Stress, Psychological / Q000097:blood / Q000517:prevention & control; D055114:X-Ray Microtomography
D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone