Molecular characterization and expression analysis of mitochondrial antiviral signaling protein gene in sea perch, Lateolabrax japonicus.
The mitochondrial antiviral signaling protein (MAVS) is vital for host defenses against viral infection by inducing expression of type I interferon. Here, the MAVS of sea perch (Lateolabrax japonicus) (LjMAVS) was cloned and analyzed. The complete cDNA sequence of LjMAVS was 3207 bp and encoded a polypeptide of 601 amino acids. LjMAVS contains an N-terminal CARD-like domain, a central proline-rich domain and a C-terminal transmembrane domain. Phylogenetic analysis indicated that LjMAVS exhibited the closest relationship to O. fasciatus MAVS. LjMAVS was ubiquitously expressed in all tested tissues of healthy fish. The expression of LjMAVS was significantly increased post nervous necrosis virus (NNV) infection in vivo in all the selected tissues. Furthermore, time course analysis showed that LjMAVS transcripts significantly increased in the brain, spleen and kidney tissues after NNV infection. LjMAVS mRNA expression was significantly up-regulated in vitro after poly I:C stimulation. The viral gene transcription of RGNNV was significantly decreased in LjMAVS over-expressing LJB cells. These findings provide useful information for further elucidating the function ofLjMAVS in antiviral innate immune against NNV in sea perch.
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: jiapeng19890219@126.com.
Peng
P
Jia
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: 369277368@qq.com.
Yilin
Y
Jin
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: 2562492896@qq.com.
Limin
L
Chen
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: zhangj358@mail2.sysu.edu.cn.
Jing
J
Zhang
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: jiakuntong@126.com.
Kuntong
K
Jia
School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: yimsh@mail.sysu.edu.cn.
Meisheng
M
Yi
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology*; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D030381:Nodaviridae / Q000276:immunology*; D010471:Perches / Q000276:immunology*; D010802:Phylogeny; D011070:Poly I-C / Q000276:immunology; D012327:RNA Virus Infections / Q000276:immunology*; D015854:Up-Regulation; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D029961:Zebrafish Proteins / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C000600526:MAVS protein, zebrafish; D029961:Zebrafish Proteins; D011070:Poly I-C
Innate immune; Mitochondrial antiviral signaling protein; Nervous necrosis virus; Sea perch