Role of the phosphatidylinositol-3-kinase/Akt/target of rapamycin pathway during ambidensovirus infection of insect cells.
The phosphatidylinositol-3-kinase (PI3K)/Akt/target of rapamycin (TOR) signalling pathway controls cell growth and survival, and is targeted by a number of viruses at different phases of their infection cycle to control translation. Whether and how insect viruses interact with this pathway remain poorly addressed. Here, we investigated the role of PI3K/Akt/TOR signalling during lethal infection of insect cells with an insect parvovirus. Using Junonia coenia densovirus (JcDV; lepidopteran ambidensovirus 1) and susceptible insect cells as experimental models, we first described JcDV cytopathology, and showed that viral infection affects cell size, cell proliferation and survival. We deciphered the role of PI3K/Akt/TOR signalling in the course of infection and found that non-structural (NS) protein expression correlates with the inhibition of TOR and the shutdown of cellular synthesis, concomitant with the burst of viral protein expression. Together, these results suggest that NS proteins control the cellular translational machinery to favour the translation of viral mRNAs at the expense of cellular mRNAs. As a consequence of TOR inhibition, cell autophagy is activated. These results highlight new functions for NS proteins in the course of multiplication of an insect parvovirus.
EPHE, Pathologie Comparée des Invertébrés, UMR 1333, 34000 Montpellier, France.|INRA, UMR 1333, 34000 Montpellier, France.
F
F
Salasc
INRA, UMR 1333, 34000 Montpellier, France.
D
D
Mutuel
INRA, UMR 1333, 34000 Montpellier, France.
S
S
Debaisieux
INRA, UMR 1333, 34000 Montpellier, France.|Invivo Agrosolutions, 06560 Valbonne, France.
A
A
Perrin
EPHE, Pathologie Comparée des Invertébrés, UMR 1333, 34000 Montpellier, France.|INRA, UMR 1333, 34000 Montpellier, France.
T
T
Dupressoir
INRA, UMR 1333, 34000 Montpellier, France.|Université de Montpellier, UMR 1333, 34000 Montpellier, France.
A-S Gosselin
AG
Grenet
INRA, UMR 1333, 34000 Montpellier, France.
M
M
Ogliastro
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D017990:Densovirus / Q000254:growth & development*; D054884:Host-Pathogen Interactions; D007313:Insecta / Q000821:virology*; D008962:Models, Theoretical; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D014176:Protein Biosynthesis; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism
D017361:Viral Nonstructural Proteins; D058539:Phosphatidylinositol 3-Kinase; D051058:Oncogene Protein v-akt; D058570:TOR Serine-Threonine Kinases