High-level aminoglycoside resistance in Enterococcus faecalis and Enterococcus faecium causing invasive infection: Twelve-year surveillance in the Minami Ibaraki Area.
We examined prevalence of high-level aminoglycoside resistance (HLAR) in Enterococcus faecalis and Enterococcus faecium causing invasive infection in the Minami Ibaraki Area. Ten strains of both species each, recovered from the blood or the cerebrospinal fluid between 2003 and 2014, were randomly selected every year. High-level resistance to gentamicin (HLR-GM) and streptomycin (HLR-SM) was detected in 34% (41 of 120 strains) and 18% (21) of E. faecalis and 9% (11) and 39% (48) of E. faecium, respectively. In comparisons of the proportions among three four-year periods, HLR-SM among E. faecium was significantly lower in the 2011-2014 period. All strains with HLR-GM were positive for the aac(6')-Ie-aph(2″)-Ia gene. The ant(6')-Ia gene was detected in all with HLR-SM except for one E. faecalis strain. The present study showed that prevalence of HLR-GM among E. faecalis and E. faecium causing invasive infection in this area was nearly equivalent to that described in previous studies in Japan and that proportions of strains with HLAR did not vary during the study period except for that of HLR-SM among E. faecium.
Department of Infectious Diseases, Tokyo Medical University Ibaraki Medical Center, Japan.
Hanako
H
Osuka
Department of Clinical Laboratories, University of Tsukuba Hospital, Japan.
Jun
J
Nakajima
Department of Infectious Diseases, Tokyo Medical University Ibaraki Medical Center, Japan.
Tsuyoshi
T
Oishi
Department of Respiratory Medicine, Tsukuba Gakuen Hospital, Japan.
Yasunori
Y
Funayama
Department of Gastroenterology and Hepatology, Ryugasaki Saiseikai Hospital, Japan.
Tsugio
T
Ebihara
Department of Respiratory Medicine, Tsukuba Medical Center Hospital, Japan.
Hiroichi
H
Ishikawa
Department of Respiratory Medicine, Tsuchiura Kyodo General Hospital, Japan.
Kazuto
K
Saito
Department of Infectious Diseases, University of Tsukuba Hospital, Japan.
Hiroshi
H
Koganemaru
Department of Infectious Diseases, University of Tsukuba Hospital, Japan. Electronic address: shitomi@md.tsukuba.ac.jp.
Shigemi
S
Hitomi
D000617:Aminoglycosides / Q000494:pharmacology / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001426:Bacterial Proteins; D024881:Drug Resistance, Bacterial; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D016984:Enterococcus faecium / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D017853:Phosphotransferases (Alcohol Group Acceptor)
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C439343:aac(6')-Im protein, Enterococcus faecium
Aminoglycoside-modifying enzyme; Enterococcus faecalis; Enterococcus faecium; Gentamicin; High-level aminoglycoside resistance; Streptomycin