A Higher Frequency of CD14+ CD169+ Monocytes/Macrophages in Patients with Colorectal Cancer.
OBJECTIVE
Monocytes and macrophages can infiltrate into tumor microenvironment and regulate the progression of tumors. This study aimed at determining the frequency of different subsets of circulating monocytes and tumor infiltrating macrophages (TIMs) in patients with colorectal cancer (CRC).


METHODS
The frequency of different subsets of circulating monocytes was characterized in 46 CRC patients and 22 healthy controls (HC) by flow cytometry. The frequency of different subsets of macrophages was analyzed in TIMs from 30 tumor tissues and in lamina propria mononuclear cells (LPMCs) from 12 non-tumor tissues. The concentrations of plasma cytokines and carcinoembryonic antigen (CEA) were determined. The potential association of these measures with the values of clinical parameters was analyzed.


RESULTS
In comparison with that in the HC, the percentages of circulating CD14+ CD169+, CD14+ CD169+ CD163+ and CD14+ CD169+ CD206+ monocytes and TIMs CD14+ CD169+ as well as IL-10+ CD14+ CD169+, but not IL-12+ CD14+ CD169+ macrophages were significantly increased, accompanied by higher levels of plasma IL-10 in the CRC patients. The percentages of CD14+ CD169+ circulating monocytes and TIM macrophages were associated with the stage of disease and correlated positively with the levels of plasma IL-10 and CEA in CRC patients.


CONCLUSIONS
Our data suggest that an increase in the frequency of CD14+ CD169+ cells may be associated with the development and progression of CRC and is concomitant rise of both, pro-tumor (M2-like, IL-10 producing) and anti-tumor (M1-like, IL-12 producing) monocytes and infiltrating macrophages. The frequency of CD14+ CD169+ circulating monocytes and infiltrating macrophages may serve as a biomarker for evaluating the pathogenic degrees of CRC.
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Chenguang
C
Li
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Xiaofan
X
Luo
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Yuyang
Y
Lin
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Xiuqi
X
Tang
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Limian
L
Ling
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Lei
L
Wang
Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China; Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, China.
Yanfang
Y
Jiang
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D007958:Leukocyte Count; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010641:Phenotype; D063267:Sialic Acid Binding Ig-like Lectin 1 / Q000378:metabolism*
D015703:Antigens, CD; D018950:Lipopolysaccharide Receptors; D063267:Sialic Acid Binding Ig-like Lectin 1