Baculovirus expression: old dog, new tricks.
Since its inception more than 30 years ago, the baculovirus expression vector system (BEVS) has been used prolifically to produce heterologous proteins for research and development. In the cell, a cornerstone of biological activity are multiprotein complexes, catalyzing essential functions. BEVS has been uniquely successful to unlock such complex assemblies for high-resolution structural and functional analysis. Synthetic biology approaches have been implemented to optimize multigene assembly methods, accelerating upstream processes. Specialized baculoviral genomes are being created with functions tailored to enhance production of particular target protein classes. Here we comment on current and emerging developments in the field and their potential to accelerate protein complex research.
a The European Molecular Biology Laboratory (EMBL); Grenoble Outstation and Unit of Virus Host-Cell Interactions (UVHCI); Université Grenoble Alpes-EMBL-CNRS ; UMR 5233; Grenoble , France.|b The School of Biochemistry; University of Bristol ; Clifton , United Kingdom.
Imre
I
Berger
c Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC); Center for Integrative Biology (CBI) ; Department of Integrated Structural Biology ; Illkirch ; France.|d Center National de la Recherche Scientifique (CNRS) UMR 7104 ; Illkirch , France.|e Institut National de la Santé et de la Recherche Médicale (INSERM) U964 ; Illkirch , France.|f Université de Strasbourg ; Strasbourg , France.
Arnaud
A
Poterszman
D016367:Baculoviridae / Q000235:genetics*; D005822:Genetic Vectors; D046912:Multiprotein Complexes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D015202:Protein Engineering / Q000379:methods / Q000639:trends; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D058615:Synthetic Biology / Q000379:methods / Q000639:trends
D046912:Multiprotein Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins
DNA recombineering; automation; baculovirus; insect cell culture; multiprotein complex; protein modification; recombinant expression; structural biology; synthetic biology