Identification of TEL-AML1 (ETV6-RUNX1) associated DNA and its impact on mRNA and protein output using ChIP, mRNA expression arrays and SILAC.
The contribution of the most common reciprocal translocation in childhood B-cell precursor leukemia t(12;21)(p13;q22) to leukemia development is still under debate. Direct as well as secondary indirect effects of the TEL-AML1 fusion protein are commonly recorded by using cell lines and patient samples, often bearing the TEL-AML1 fusion protein for decades. To identify direct targets of the fusion protein a short-term induction of TEL-AML1 is needed. We here describe in detail the experimental procedure, quality controls and contents of the ChIP, mRNA expression and SILAC datasets associated with the study published by Linka and colleagues in the Blood Cancer Journal [1] utilizing a short term induction of TEL-AML1 in an inducible precursor B-cell line model.
Heinrich-Heine-University Düsseldorf, Medical Faculty, Clinic for Pediatric Oncology, Hematology and Clinical Immunology, Duesseldorf, Germany.
Yvonne
Y
Linka
Heinrich-Heine-University Düsseldorf, Medical Faculty, Clinic for Pediatric Oncology, Hematology and Clinical Immunology, Duesseldorf, Germany ; Department of Computer Science, Bonn-Rhine-Sieg University of Applied Sciences, St. Augustin, Germany.
Sebastian
S
Ginzel
Heinrich-Heine-University Düsseldorf, Medical Faculty, Clinic for Pediatric Oncology, Hematology and Clinical Immunology, Duesseldorf, Germany.
Arndt
A
Borkhardt
Heinrich-Heine-University Düsseldorf, Medical Faculty, Clinic for Pediatric Oncology, Hematology and Clinical Immunology, Duesseldorf, Germany.
Pablo
P
Landgraf
B-cell leukemia; ETV6-RUNX1; Genomics; TEL-AML1; Translocation