A two-photon fluorescent probe for lysosomal zinc ions.
The selective detection of zinc ions in lysosomes over that in cytosol is achieved with a fluorescent probe, which enabled the fluorescence imaging of endogenous zinc ions in lysosomes of NIH 3T3 cells as well as mouse hippocampal tissues by two-photon microscopy under excitation at 900 nm.
Department of Chemistry, Kyungpook National University, Daegu 702-701, Republic of Korea. cwcho@knu.ac.kr.
Hyo-Jun
HJ
Lee
Department of Chemistry, Kyungpook National University, Daegu 702-701, Republic of Korea. cwcho@knu.ac.kr.
Chang-Woo
CW
Cho
Department of Chemistry, POSTECH, Pohang 790-784, Republic of Korea. ahn@postech.ac.kr.
Hyewon
H
Seo
Department of Chemistry, POSTECH, Pohang 790-784, Republic of Korea. ahn@postech.ac.kr.
Subhankar
S
Singha
Department of Chemistry, POSTECH, Pohang 790-784, Republic of Korea. ahn@postech.ac.kr.
Yong Woong
YW
Jun
Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang 790-784, Republic of Korea.
Kyung-Ha
KH
Lee
Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang 790-784, Republic of Korea.
Youngseob
Y
Jung
Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang 790-784, Republic of Korea.
Kyong-Tai
KT
Kim
School of Life Sciences, UNIST, Ulsan 689-798, Republic of Korea.
Seongjun
S
Park
School of Life Sciences, UNIST, Ulsan 689-798, Republic of Korea.
Sung Chul
SC
Bae
Department of Chemistry, POSTECH, Pohang 790-784, Republic of Korea. ahn@postech.ac.kr.
Kyo Han
KH
Ahn
D000818:Animals; D002413:Cations, Divalent / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry*; D008247:Lysosomes / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D061848:Optical Imaging; D015032:Zinc / Q000032:analysis*
D002413:Cations, Divalent; D005456:Fluorescent Dyes; D015032:Zinc