BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis.
Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol-cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol-cytochrome c reductase iron-sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants. These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth. qRT-PCR results revealed that in 35S::BcRISP1 plants, the expression levels of the mitochondrial respiratory chain-related genes, COX10 and RIP1, were enhanced, whereas the expression levels of QCR7 and SDH2-1 were reduced. This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore, reducing the seed set.
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Tongkun
T
Liu
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Yu
Y
Qian
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Weike
W
Duan
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Jun
J
Ren
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Xilin
X
Hou
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China ; State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture , Nanjing 210095, China ; Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture , Nanjing 210095, China.
Ying
Y
Li