IgE Epitope Mapping Using Peptide Microarray Immunoassay.
IgE epitope mapping of food allergens contributes to a better understanding of the pathogenesis of food allergy and may become an additional tool for food allergy diagnosis/prognosis. Microarray platforms which allow for simultaneous screening of a large number of peptides corresponding to the sequences of food allergens are ideally suited for large-scale IgE epitope mapping. Here we describe the method of performing a reliable and sensitive peptide microarray immunoassay, which was developed in our lab and results in the identification of candidate IgE epitope biomarkers useful in determining allergic disease severity and prognosis, as well as in the prediction of treatment outcomes. A gastric digestion model that can be applied to prescreen peptides and reduce costs in the peptide microarray is also described in this chapter.
Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, Box 1089, One Gustave L. Levy Place, New York, NY, 10029-6574, USA.
Gustavo
G
Gimenez
Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, Box 1089, One Gustave L. Levy Place, New York, NY, 10029-6574, USA.|Institute of Food Science Research (CIAL) CSIC-UAM, Madrid, Spain.
Sara
S
Benedé
Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, Box 1089, One Gustave L. Levy Place, New York, NY, 10029-6574, USA. jing.s.lin@mssm.edu.
Jing
J
Lin
D053000:Analytic Sample Preparation Methods; D000818:Animals; D018604:Epitope Mapping / Q000379:methods*; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D007118:Immunoassay / Q000379:methods*; D007073:Immunoglobulin E / Q000276:immunology*; D007150:Immunohistochemistry; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011327:Printing; D040081:Protein Array Analysis / Q000379:methods*; D059748:Proteolysis; D013223:Statistics as Topic; D013552:Swine
D010455:Peptides; D007073:Immunoglobulin E
Epitope mapping; Food allergy; Gastric digestion; IgE; Immunoassay; Peptide microarray