Characterization and isolation of immature neurons of the adult mouse piriform cortex.
Physiological studies indicate that the piriform or primary olfactory cortex of adult mammals exhibits a high degree of synaptic plasticity. Interestingly, a subpopulation of cells in the layer II of the adult piriform cortex expresses neurodevelopmental markers, such as the polysialylated form of neural cell adhesion molecule (PSA-NCAM) or doublecortin (DCX). This study analyzes the nature, origin, and potential function of these poorly understood cells in mice. As previously described in rats, most of the PSA-NCAM expressing cells in layer II could be morphologically classified as tangled cells and only a small proportion of larger cells could be considered semilunar-pyramidal transitional neurons. Most were also immunoreactive for DCX, confirming their immature nature. In agreement with this, detection of PSA-NCAM combined with that of different cell lineage-specific antigens revealed that most PSA-NCAM positive cells did not co-express markers of glial cells or mature neurons. Their time of origin was evaluated by birthdating experiments with halogenated nucleosides performed at different developmental stages and in adulthood. We found that virtually all cells in this paleocortical region, including PSA-NCAM-positive cells, are born during fetal development. In addition, proliferation analyses in adult mice revealed that very few cells were cycling in layer II of the piriform cortex and that none of them was PSA-NCAM-positive. Moreover, we have established conditions to isolate and culture these immature neurons in the adult piriform cortex layer II. We find that although they can survive under certain conditions, they do not proliferate in vitro either. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 748-763, 2016.
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.|Centro De Investigaciones Biomédicas En Red Sobre Enfermedades Neurodegenerativas (CIBERNED), Spain.
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Rubio
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.
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Belles
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.|Centro De Investigaciones Biomédicas En Red Sobre Enfermedades Neurodegenerativas (CIBERNED), Spain.
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Belenguer
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.
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Vidueira
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.|Centro De Investigaciones Biomédicas En Red Sobre Enfermedades Neurodegenerativas (CIBERNED), Spain.
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Fariñas
Departamento De Biología Celular, Universidad De Valencia, Burjassot, 46100, Spain.|CIBERSAM: Spanish National Network for Research in Mental Health, Spain.|Fundación Investigación Hospital Clínico De Valencia, INCLIVA, Valencia, 46010, Spain.
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Nacher
D000367:Age Factors; D000818:Animals; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005260:Female; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism*; D066195:Piriform Cortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D011247:Pregnancy; D012794:Sialic Acids / Q000378:metabolism*
C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D039842:Neural Cell Adhesion Molecule L1; D009479:Neuropeptides; D012794:Sialic Acids; C103469:polysialyl neural cell adhesion molecule
PSA-NCAM; adult neurogenesis; neuroblast; neuronal birthdating; proliferation