Intrafollicular expression and potential regulatory role of cocaine- and amphetamine-regulated transcript in the ovine ovary.
Follicular growth is regulated by a complex interaction of pituitary gonadotropins with local regulatory molecules. Previous studies demonstrated an important role for cocaine- and amphetamine-regulated transcript (CART) in regulation of granulosa cell estradiol production associated with dominant follicle selection in cattle. However, intraovarian expression and actions of CART in other species, including sheep, are not known. The objective of this study was to investigate the expression of CART in sheep follicles and determine the effects of CART on indices of ovine granulosa cell function linked to follicular development. Results demonstrated the expression of CART messenger RNA and prominent intraovarian localization of CART peptide in granulosa cells of sheep follicles. Granulosa cell CART messenger RNA was lower, but follicular fluid estradiol concentrations were higher in large (>5 mm) follicles vs smaller 3- to 5-mm follicles harvested from sheep ovaries of abattoir origin. CART treatment inhibited follicle stimulating hormone-induced estradiol production by cultured ovine granulosal cells and also blocked the follicle stimulating hormone-induced increase in granulosa cell numbers. Results demonstrate expression of CART in sheep follicular tissues and suggest potential biological actions of CART, which are inhibitory to ovine follicular growth and development.
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
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Y
Huang
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
X L
XL
Yao
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
J Z
JZ
Meng
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Y
Y
Liu
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
X L
XL
Jiang
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
J W
JW
Chen
College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
P F
PF
Li
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Y S
YS
Ren
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
W Z
WZ
Liu
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Department of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA.
J B
JB
Yao
Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, MI 48824, USA; Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA.
J K
JK
Folger
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Laboratory of Mammalian Reproductive Biology and Genomics, Michigan State University, East Lansing, MI 48824, USA; Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA; Department of Physiology, Michigan State University, East Lansing, MI 48824, USA.
G W
GW
Smith
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi 030801, China. Electronic address: lihualvsxau@126.com.
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LH
Lv
D000818:Animals; D001483:Base Sequence; D002452:Cell Count; D004958:Estradiol / Q000032:analysis / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D015571:Follicular Fluid / Q000737:chemistry; D015870:Gene Expression; D006107:Granulosa Cells / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology*; D006080:Ovarian Follicle / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D010053:Ovary / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D017385:Sequence Homology; D012756:Sheep / Q000378:metabolism*; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein; D004958:Estradiol; D005640:Follicle Stimulating Hormone
CART; Estradiol; Follicle; Granulosal cell; Sheep