Investigating the influence of chromatic aberration and optical illumination bandwidth on fundus imaging in rats.
Abstract. Rodent models are indispensable in studying various retinal diseases. Noninvasive, high-resolution retinal imaging of rodent models is highly desired for longitudinally investigating the pathogenesis and therapeutic strategies. However, due to severe aberrations, the retinal image quality in rodents can be much worse than that in humans. We numerically and experimentally investigated the influence of chromatic aberration and optical illumination bandwidth on retinal imaging. We confirmed that the rat retinal image quality decreased with increasing illumination bandwidth. We achieved the retinal image resolution of 10  μm using a 19 nm illumination bandwidth centered at 580 nm in a home-built fundus camera. Furthermore, we observed higher chromatic aberration in albino rat eyes than in pigmented rat eyes. This study provides a design guide for high-resolution fundus camera for rodents. Our method is also beneficial to dispersion compensation in multiwavelength retinal imaging applications.
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Hao
H
Li
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Wenzhong
W
Liu
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United StatesbNorthwestern University, Department of Ophthalmology, 645 North Michigan Avenue, Chicago, Illinois 60611, United States.
Hao F
HF
Zhang
D000417:Albinism; D000465:Algorithms; D000818:Animals; D016477:Artifacts; D003116:Color; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005654:Fundus Oculi; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008029:Lighting / Q000379:methods*; D051381:Rats; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012160:Retina / Q000033:anatomy & histology*; D042262:Retinoscopy / Q000379:methods*; D012680:Sensitivity and Specificity