Intraoperative measurement of bowel oxygen saturation using a multispectral imaging laparoscope.
Intraoperative monitoring of tissue oxygen saturation (StO2 ) has potentially important applications in procedures such as organ transplantation or colorectal surgery, where successful reperfusion affects the viability and integrity of repaired tissues. In this paper a liquid crystal tuneable filter-based multispectral imaging (MSI) laparoscope is described. Motion-induced image misalignments are reduced, using feature-based registration, before regression of the tissue reflectance spectra to calculate relative quantities of oxy- and deoxyhaemoglobin. The laparoscope was validated in vivo, during porcine abdominal surgery, by making parallel MSI and blood gas measurements of the small bowel vasculature. Ischaemic conditions were induced by local occlusion of the mesenteric arcade and monitored using the system. The MSI laparoscope was capable of measuring StO2 over a wide range (30-100%) with a temporal error of ± 7.5%. The imager showed sensitivity to spatial changes in StO2 during dynamic local occlusions, as well as tracking the recovery of tissues post-occlusion.
Department of Surgery and Cancer, Imperial College London, SW7 2AZ, UK ; Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, SW7 2AZ, UK.
Neil T
NT
Clancy
Department of Surgery and Cancer, Imperial College London, SW7 2AZ, UK.
Shobhit
S
Arya
Centre for Medical Image Computing, Department of Computer Science, University College London, WC1E 6BT, UK.
Danail
D
Stoyanov
Department of Surgery and Cancer, Imperial College London, SW7 2AZ, UK ; Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, SW7 2AZ, UK.
Mohan
M
Singh
Department of Surgery and Cancer, Imperial College London, SW7 2AZ, UK.
George B
GB
Hanna
Department of Surgery and Cancer, Imperial College London, SW7 2AZ, UK ; Hamlyn Centre for Robotic Surgery, Institute of Global Health Innovation, Imperial College London, SW7 2AZ, UK.
Daniel S
DS
Elson
(110.4234) Multispectral and hyperspectral imaging; (170.0170) Medical optics and biotechnology; (170.1610) Clinical applications; (170.2150) Endoscopic imaging; (170.2655) Functional monitoring and imaging; (170.6510) Spectroscopy, tissue diagnostics