Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model.
In this study, gold nanoparticles (AuNPs) were used as carriers of the signaling anti-chicken antibody peroxidase in comparison with anti-chicken antibody peroxidase without gold nanoparticle in a commercial avian influenza kit. AuNPs enhanced the absorbance and shortened the assay time. AuNPs act as a carrier of many enzymes and multiply the effect of enzyme when reacting with substrate. They amplify optical signal, while keeping low background signals.
1 Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute , Karaj, Iran .|3 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Tara
T
Emami
1 Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute , Karaj, Iran .
Rasool
R
Madani
1 Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute , Karaj, Iran .
Fariba
F
Golchinfar
2 Department of Poultry Disease Research and Diagnosis, Razi Vaccine and Serum Research Institute , Karaj, Iran .
Abdolhamid
A
Shoushtary
3 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences , Tehran, Iran .
Seyed Mohammad
SM
Amini
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002645:Chickens / Q000276:immunology / Q000821:virology; D004337:Drug Carriers / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006046:Gold / Q000008:administration & dosage*; D005585:Influenza in Birds / Q000276:immunology; D053768:Metal Nanoparticles / Q000008:administration & dosage*; D009195:Peroxidase / Q000276:immunology
D000906:Antibodies; D004337:Drug Carriers; D006046:Gold; D009195:Peroxidase