HYALURONIC ACID IN DERMAL REJUVENATION: AN IN VITRO STUDY.
The purpose of this paper is to evaluate the role of hyaluronic acid in bio-revitalization by testing several extracellular matrix biological parameters in cultured dermal fibroblasts. To this aim, fibroblastic expressed genes after exposition to three hyaluronic acid medical devices were evaluated. Cells were seeded on a layer of three different medical devices containing 6.2, 10 and 20 mg/ml of hyaluronic acid for 24 h. Real Time Polymerase Chain Reaction was performed to investigate gene expressions. Genes encoding hyaluronic acid synthesis and degradation, Metalloproteinases 2 and 3 and Desmoplakin production as well as GDF6, and IGF1 were activated by hyaluronic acid products. The in vitro study showed similar effects on tested genes despite a different concentration of hyaluronic acid contained in the medical devices and the simultaneous presence of other additives. Based on the reported data, gene activations are an aspect of metabolic modulation of signalling pathways rather than the proportional production of a specific connective tissue molecule. Indeed different hyaluronic acid concentration and the presence of other additives did not change the overall effect on the studied genes. We believe that the optimization of extracellular matrix micro-environment, obtained by enhanced structural support with hyaluronic acid, leads to functional and metabolic improvement.
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
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Avantaggiato
Department of Biomedicine and Prevention, University of Tor Vergata, Roma, Italy.
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Pascali
Department of Translational Medicine and Surgery, Neuroscience Center of Milan, University of Milano Bicocca, Monza, Italy.
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Lauritano
Department of Surgical and Morphological Sciences, University of Insubria, Varese, Italy.
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Cura
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
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Pezzetti
Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
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Palmieri