Microarray analysis of Neosartorya fischeri using different carbon sources, petroleum asphaltenes and glucose-peptone.
Asphaltenes are considered as the most recalcitrant petroleum fraction and represent a big problem for the recovery, separation and processing of heavy oils and bitumens. Neosartorya fischeri is a saprophytic fungus that is able to grow using asphaltenes as the sole carbon source [1]. We performed transcription profiling using a custom designed microarray with the complete genome from N. fischeri NRRL 181 in order to identify genes related to the transformation of asphaltenes [1]. Data analysis was performed using the genArise software. Results showed that 287 genes were up-regulated and 118 were down-regulated. Here we describe experimental procedures and methods about our dataset (NCBI GEO accession number GSE68146) and describe the data analysis to identify different expression levels in N. fischeri using this recalcitrant carbon source.
Instituto de Biotecnología, UNAM, Av. Universidad 2001 Cuernavaca, Mor., Mexico.
Edna L
EL
Hernández-López
Centro de Ciencias Genómicas UNAM, Av. Universidad s/n, Col. Chamilpa, Cuernavaca, Mor., Mexico.
Shamayim T
ST
Ramírez-Puebla
Centro de Nanociencias y Nanotecnología, UNAM, Km 107 carretera Tijuana-Ensenada, Baja California 22860, Mexico.
Rafael
R
Vazquez-Duhalt
Asphaltenes; Biotransformation; Cytochrome P450; Neosartorya