Rhodiola rosea extends lifespan and improves stress tolerance in silkworm, Bombyx mori.
The root of Rhodiola rosea is widely used in Traditional Chinese Medicine. The extract from R. rosea is reported to extend the lifespan of yeast, nematode, and fruit fly. However, the molecular mechanism is not fully understood. Here, we tested whether R. rosea extends the lifespan of the silkworm. An aqueous extract of R. rosea significantly prolonged the lifespan of the silkworm, without affecting its daily food intake, body weight, or fecundity, suggesting that R. rosea did not exhibit obvious side effects. Rhodiola rosea extract also enhanced the stress resistance in the silkworm, against heat stress (37 °C) and starvation. The R. rosea extract increased the activity of the major antioxidant enzymes, glutathione S-transferase and catalase, and altered the content of glutathione and malondialdehyde. Rhodiola rosea increased the expression of BmFoxO, which is a downstream regulator of insulin/IGF-1 signaling (IIS) pathway in the silkworm. Our results showed that R. rosea extends lifespan, in which IIS pathway might be involved, and enhances stress resistance in the silkworm. Thus, the silkworm might be used as a novel animal model for lifespan study and efficacy evaluation of Traditional Chinese Medicines.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Cong
C
Chen
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Jiangbo
J
Song
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Min
M
Chen
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Zhiquan
Z
Li
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Xiaoling
X
Tong
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Hai
H
Hu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Zhonghuai
Z
Xiang
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China.
Cheng
C
Lu
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China. fydai@swu.edu.cn.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, 400716, China. fydai@swu.edu.cn.
Fangyin
F
Dai
D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D012831:Bombyx / Q000502:physiology*; D005247:Feeding Behavior / Q000187:drug effects; D005298:Fertility / Q000187:drug effects; D008136:Longevity / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D029771:Rhodiola / Q000737:chemistry*; D013312:Stress, Physiological / Q000187:drug effects*
D010936:Plant Extracts
Bombyx mori; IIS pathway; Lifespan; Oxidative stress; Rhodiola rosea