CD1c(+) myeloid dendritic cells in myeloid neoplasia.
We determined the normal level and phenotype of CD1c(+) myeloid dendritic cells (MDCs) in blood and bone marrow and evaluated the level of CD1c(+) MDCs in 295 myeloid neoplasms. CD1c(+) MDCs were increased above the mean level of non-neoplastic hospital controls in 18.0% (53/295) of myeloid malignancies, increased three standard deviations above the control mean in 14.2% (42/295) with a 10-fold or more increase compared to mean in 6.8% (20/295). Increased CD1c(+) MDCs were associated with chronic myelomonocytic leukemia (CMML) (12/24, 50%) and acute myeloid leukemia (AML) (31/140, 22%) with a strong association with AML with the inv(16) cytogenetic abnormality. The cells were not increased in chronic myelogenous leukemia (CML) and rarely increased in non-CML myeloproliferative neoplasms (MPN) and myelodysplastic syndromes (MDS). Immunohistochemical staining of cases with increased CD1c(+) MDCs did not reveal clustering of the cells unlike that observed with myeloid neoplasms associated with increased plasmacytoid dendritic cells. Our findings indicate CD1c(+) MDC elevations are not uncommon in myeloid leukemias and are associated with CMML and AML, particularly AML with inv(16). © 2015 International Clinical Cytometry Society.
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Howard J
HJ
Meyerson
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Ebenezer
E
Osei
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Karen
K
Schweitzer
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Georgetta
G
Blidaru
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Alison
A
Edinger
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
June
J
Schlegelmilch
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Amad
A
Awadallah
Department of Pathology, University Hospitals Case Medical Center and Seidman Cancer Center Case Western Reserve University, Cleveland, Ohio, 44106.
Tanu
T
Goyal
D018949:Antigens, CD1 / Q000378:metabolism*; D003713:Dendritic Cells / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D015477:Leukemia, Myelomonocytic, Chronic / Q000175:diagnosis / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000473:pathology; D022423:Myeloid Cells / Q000378:metabolism* / Q000473:pathology; D009196:Myeloproliferative Disorders / Q000473:pathology*
D018949:Antigens, CD1; C437944:CD1C protein, human; D006023:Glycoproteins
CD1c; flow cytometry; leukemia; myeloid dendritic cell