Serum concentration and skin tissue expression of insulin-like growth factor 2 in canine generalized demodicosis.
BACKGROUND
There is increasing evidence that insulin-like growth factor-2 (IGF-2) levels are altered in skin injury; there are no data evaluating the serum concentration and skin tissue expression of IGF-2 in canine generalized demodicosis.


OBJECTIVE
To assess serum concentrations of IGF-2 collected from dogs with generalized demodicosis compared to healthy dogs and to determine the location of IGF-2 in the skin of affected dogs.


METHODS
Blood and skin samples were collected from 12 dogs of differing breeds and gender at 1-2 years of age that had a confirmed diagnosis of generalized demodicosis. Age-matched control skin and blood samples were collected from 11 normal dogs of different breeds and gender. Serum IGF-2 concentrations were measured by enzyme-linked immunosorbent assay. Skin tissue expression of IGF-2 was analysed by immunohistochemical methods.


RESULTS
Serum concentration and skin tissue expression of IGF-2 were increased in dogs with generalized demodicosis compared with control dogs.


CONCLUSIONS
These findings indicate that keratinocytes, histiocytes and fibrocytes in the dermis are positive for IGF-2; they may be a source of the elevated serum IGF-2 levels in dogs with generalized demodicosis.
Department of Biochemistry, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Gul F
GF
Yarim
Department of Internal Medicine, Faculty of Veterinary Medicine, Kirikkale University, Yahsihan, 71450, Kirikkale, Turkey.
Bugrahan B
BB
Yagci
Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Murat
M
Yarim
Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Mahmut
M
Sozmen
Department of Internal Medicine, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Didem
D
Pekmezci
Department of Biochemistry, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Sena
S
Cenesiz
Department of Preclinical Sciences, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Gokmen Z
GZ
Pekmezci
Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55200, Samsun, Turkey.
Efe
E
Karaca
D000818:Animals; D016022:Case-Control Studies; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism / Q000469:parasitology*; D004285:Dogs; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008924:Mite Infestations / Q000662:veterinary; D012867:Skin / Q000378:metabolism*
D007335:Insulin-Like Growth Factor II