Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim.
In this study, the extraction of water-soluble polysaccharides (BDPs) from Berberis dasystachya Maxim using dynamic microwave-assisted extraction (DMAE) was discussed. A Box-Behnken design combined with response surface methodology has been employed to optimize extraction parameters of DMAE. The BDPs have been analyzed in order to identify a variety of chemical properties. Antioxidant and anti-tumor activities in vitro have been studied by DPPH, ABTS, reducing power assay, and MTT assay, respectively. The results obtained showed that the optimal extraction conditions were as follows: ratio of water to raw material (X1) 25.84 mg/L, extraction power (X2) 433.13W, extraction time (X3) 35.18 min, and the maximum yield of extraction was 6.472 ± 0.384%, which was in good agreement with the predicted value. The physicochemical tests demonstrated that the BDPs mainly consist of rhamnose, arabinose, xylose, mannose, glucose and lactose in a molar ratio of 1:17.3:1.33:7:2.33:1.78; the average molecular weight of the BDPs was estimated to be from 2.95×10(5) and 1.52×10(3)Da, respectively. Furthermore, the BDPs exhibited effective antioxidant and anti-proliferative properties in vitro. Such pharmaceutical activities could prove useful for potential future applications involving the berries of B. dasystachya Maxim.
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Lijuan
L
Han
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810001, PR China; Qinghai University, Xining, Qinghai 810001, PR China. Electronic address: yrsuo@nwipb.cas.cn.
Yourui
Y
Suo
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Yongjing
Y
Yang
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Jing
J
Meng
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810001, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Na
N
Hu
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D028142:Berberis / Q000737:chemistry*; D045744:Cell Line, Tumor; D005591:Chemical Fractionation / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides
Antioxidant and anti-proliferative; B. dasystachya Maxim polysaccharides; Response surface methodology