Characterization, Genome Sequence, and Analysis of Escherichia Phage CICC 80001, a Bacteriophage Infecting an Efficient L-Aspartic Acid Producing Escherichia coli.
Escherichia phage CICC 80001 was isolated from the bacteriophage contaminated medium of an Escherichia coli strain HY-05C (CICC 11022S) which could produce L-aspartic acid. The phage had a head diameter of 45-50 nm and a tail of about 10 nm. The one-step growth curve showed a latent period of 10 min and a rise period of about 20 min. The average burst size was about 198 phage particles per infected cell. Tests were conducted on the plaques, multiplicity of infection, and host range. The genome of CICC 80001 was sequenced with a length of 38,810 bp, and annotated. The key proteins leading to host-cell lysis were phylogenetically analyzed. One protein belonged to class II holin, and the other two belonged to the endopeptidase family and N-acetylmuramoyl-L-alanine amidase family, respectively. The genome showed the sequence identity of 82.7% with that of Enterobacteria phage T7, and carried ten unique open reading frames. The bacteriophage resistant E. coli strain designated CICC 11021S was breeding and its L-aspartase activity was 84.4% of that of CICC 11022S.
China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China. xuyqskywalker@163.com.|Engineering Technology Research Center of Fumaric Acid Biotransformation in Shandong Province, Yantai, Shandong Province, 265709, People's Republic of China. xuyqskywalker@163.com.
Youqiang
Y
Xu
Engineering Technology Research Center of Fumaric Acid Biotransformation in Shandong Province, Yantai, Shandong Province, 265709, People's Republic of China.
Yuyue
Y
Ma
China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China.
Su
S
Yao
Engineering Technology Research Center of Fumaric Acid Biotransformation in Shandong Province, Yantai, Shandong Province, 265709, People's Republic of China.
Zengyan
Z
Jiang
China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China.|Engineering Technology Research Center of Fumaric Acid Biotransformation in Shandong Province, Yantai, Shandong Province, 265709, People's Republic of China.
Jiangsen
J
Pei
China Center of Industrial Culture Collection, China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People's Republic of China. cheng100027@163.com.
Chi
C
Cheng
D001224:Aspartic Acid / Q000378:metabolism*; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny
D001224:Aspartic Acid
Escherichia phage; Genome comparison; Genome sequencing; L-Aspartic acid; Phage holin; Phage lysin