MtiBase: a database for decoding microRNA target sites located within CDS and 5'UTR regions from CLIP-Seq and expression profile datasets.
MicroRNAs (miRNAs) play an important role in the regulation of gene expression. Previous studies on miRNA functions mainly focused on their target sites in the 3' untranslated regions (UTRs) of mRNAs. However, increasing evidence has revealed that miRNAs can also induce mRNA degradation and mediate translational repression via complementary interactions with the coding sequence (CDS) and 5'UTR of mRNAs. In this study, we developed a novel database, MtiBase, to facilitate the comprehensive exploration of CDS- and 5'UTR-located miRNA target sites identified from cross-linking immunoprecipitation sequencing (CLIP-Seq) datasets and to uncover their regulatory effects on mRNA stability and translation from expression profile datasets. By integrating 61 Argonaute protein-binding CLIP-Seq datasets and miRNA target sites predicted by five commonly used programs, we identified approximately 4 400 000 CDS-located and 470 000 5'UTR-located miRNA target sites. Moreover, we evaluated the regulatory effects of miRNAs on mRNA stability and translation using the data from 222 gene expression profiles, and 28 ribosome-protected fragment sequencing, and six pulsed stable isotope labeling with amino acids in culture. Finally, the effects of SNPs on the functions of miRNA target sites were systematically evaluated. Our study provides a useful tool for functional studies of miRNAs in regulating physiology and pathology. Database URL: http://mtibase.sysu.edu.cn.
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol.
Zhi-Wei
ZW
Guo
Key Laboratory of Liver Disease of Guangdong Province, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Chen
C
Xie
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, USA and.
Jian-Rong
JR
Yang
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol.
Jun-Hao
JH
Li
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, yangjh7@mail.sysu.edu.cn.
Jian-Hua
JH
Yang
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Guangzhou, P. R. China yangjh7@mail.sysu.edu.cn.
Limin
L
Zheng
D020121:5' Untranslated Regions / Q000235:genetics*; D000818:Animals; D030561:Databases, Nucleic Acid; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D058977:Molecular Sequence Annotation; D016366:Open Reading Frames / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017423:Sequence Analysis, RNA; D013223:Statistics as Topic
D020121:5' Untranslated Regions; D035683:MicroRNAs