Profiling of Yeast Lipids by Shotgun Lipidomics.
Lipidomics is a rapidly growing technology for identification and quantification of a variety of cellular lipid molecules. Following the successful development and application of functional genomic technologies in yeast Saccharomyces cerevisiae, we witness a recent expansion of lipidomics applications in this model organism. The applications include detailed characterization of the yeast lipidome as well as screening for perturbed lipid phenotypes across hundreds of yeast gene deletion mutants. In this chapter, we describe sample handling, mass spectrometry, and bioinformatics methods developed for yeast lipidomics studies.
Lipotype GmbH, Tatzberg 47, Dresden, 01307, Germany. klose@lipotype.com.
Christian
C
Klose
Department of Biochemistry and Molecular Medicine, Université de Montréal, C.P. 6128, Succ. Centre-ville, Montréal, QC, Canada, H3T 1J4. kirill.tarasov@outlook.com.
Kirill
K
Tarasov
D019295:Computational Biology / Q000379:methods*; D050356:Lipid Metabolism / Q000235:genetics; D008055:Lipids / Q000096:biosynthesis* / Q000235:genetics; D013058:Mass Spectrometry / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D008055:Lipids
Lipidomics; Lipids; Mass spectrometry; Yeast