Characterization of a novel laccase purified from the fungus Hohenbuehelia serotina and its decolourisation of dyes.
A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa. The enzyme showed activity towards several substrates, the most sensitive of which was 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). The highest enzymatic activity using ABTS as a substrate was observed at pH 6.8 and 30°C. The enzyme activity was found to be significantly enhanced in the presence of Zn(2+) ions and inhibited by Fe(2+) ions. Moreover, SDS and β-mercaptoethanol were inhibitory, and inhibition by L-cysteine was observed while EDTA and DMSO had almost no inhibitory effect. The laccase could effectively decolorize seven different dyes within 30 minutes at 40°C.
State Key Laboratory for Agro-biotechnology, Department of Microbiology and Immunology, China Agricultural University. Beijing, People's Republic of China.
Yingyin
Y
Xu
Department of Envioronmental and Chemical Engenineering, Tangshan College, People's Republic of China.
Yuxiao
Y
Lu
State Key Laboratory for Agro-biotechnology, Department of Microbiology and Immunology, China Agricultural University. Beijing, People's Republic of China.
Rui
R
Zhang
State Key Laboratory for Agro-biotechnology, Department of Microbiology and Immunology, China Agricultural University. Beijing, People's Republic of China.
Hexiang
H
Wang
State Key Laboratory for Agro-biotechnology, Department of Microbiology and Immunology, China Agricultural University. Beijing, People's Republic of China.
Qinghong
Q
Liu
D001487:Basidiomycota / Q000201:enzymology*; D052160:Benzothiazoles / Q000737:chemistry*; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry; D004795:Enzyme Stability; D048690:Fruiting Bodies, Fungal / Q000201:enzymology; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D008970:Molecular Weight; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry*
D052160:Benzothiazoles; D004396:Coloring Agents; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D042845:Laccase