Regulation by Phloroglucinol of Nrf2/Maf-Mediated Expression of Antioxidant Enzymes and Inhibition of Osteoclastogenesis via the RANKL/RANK Signaling Pathway: In Silico study.
BACKGROUND
Phloroglucinol is an antioxidant compound with many positive effects on health. The purpose of this study was to determine the role of phloroglucinol in osteoclastogenesis via the RANKL/RANK signaling pathway and the activity of the transcription factor Nrf2.


METHODS
Analysis was performed in silico using the primary method of docking by the use of Hex 8.0 software and Haddock web server. Analysis of interactions was then performed to determine interactions between the ligand and its receptors by using the software LigPlus and LigandScout 3.1.


RESULTS
Results indicated that phloroglucinol compound was thought to inhibit osteoclastogenesis via three mechanisms: inhibiting RANKL-RANK interaction, sustaining the RANKL-OPG bond, and increasing the activity of the transcription factor Nrf2.
Department of Orthopaedics and Traumatology, Hasan Sadikin General Hospital, Medical Faculty Padjadjaran University, Bandung, West Java, Indonesia.
Agus Hadian
AH
Rahim
Research Center for Osteoporosis, Department of Medical Chemistry and Biochemistry, Medical Faculty Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Bambang
B
Setiawan
Malang In Silico Club, Malang, East Java, Indonesia.
Firli Rahmah Primula
FR
Dewi
Research Center for Osteoporosis, Department of Orthopaedics and Traumatology, Ulin General Hospital, Medical Faculty Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia.
Zairin
Z
Noor
Nrf2; RANK; RANKL; in silico; osteoclastogenesis; phloroglucinol