Nondestructive detection of lead chrome green in tea by Raman spectroscopy.
Raman spectroscopy was first adopted for rapid detecting a hazardous substance of lead chrome green in tea, which was illegally added to tea to disguise as high-quality. 160 samples of tea infusion with different concentrations of lead chrome green were prepared for Raman spectra acquirement in the range of 2804 cm(-1)-230 cm(-1) and the spectral intensities were calibrated with relative intensity standards. Then wavelet transformation (WT) was adopted to extract information in different time and frequency domains from Raman spectra, and the low-frequency approximation signal (ca4) was proved as the most important information for establishment of lead chrome green measurement model, and the corresponding partial least squares (PLS) regression model obtained good performance in prediction with Rp and RMSEP of 0.936 and 0.803, respectively. To further explore the important wavenumbers closely related to lead chrome green, successive projections algorithm (SPA) was proposed. Finally, 8 characteristic wavenumbers closely related to lead chrome green were obtained and a more convenient and fast model was also developed. These results proved the feasibility of Raman spectroscopy for nondestructive detection of lead chrome green in tea quality control.
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Xiao-Li
XL
Li
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Chan-Jun
CJ
Sun
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Liu-Bin
LB
Luo
College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
Yong
Y
He
D000465:Algorithms; D002138:Calibration; D002840:Chromates / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D015386:Hazardous Substances / Q000737:chemistry*; D007854:Lead / Q000737:chemistry*; D016018:Least-Squares Analysis; D008962:Models, Theoretical; D011786:Quality Control; D013059:Spectrum Analysis, Raman / Q000379:methods; D013662:Tea / Q000033:anatomy & histology*
D002840:Chromates; D004396:Coloring Agents; D015386:Hazardous Substances; D013662:Tea; D007854:Lead; C031895:lead chromate