Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.
Using Drosophila actin-binding protein Dunc-115 as model system, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single-cell-level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.
Department of Biology, Brooklyn College, City University of New York, 209 Ingersoll Hall Extension, 2900 Bedford Avenue, Brooklyn, NY, 11210, USA. QHe@brooklyn.cuny.edu.
Qi
Q
He
Department of Biological Sciences and Geology, Queensborough Community College, City University of New York, Bayside, NY, USA.
Christopher
C
Roblodowski
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology* / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D029721:Drosophila Proteins / Q000276:immunology / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D005123:Eye / Q000378:metabolism; D005260:Female; D007814:Larva / Q000378:metabolism; D008297:Male
D000199:Actins; D000906:Antibodies; D029721:Drosophila Proteins
Actin-binding protein; Confocal microscopy; Drosophila; Dunc-115; MARCM