Short ragweeds is highly cross-reactive with other ragweeds.
BACKGROUND
The most widespread ragweed (Ambrosia) species in North America are short ragweed (Ambrosia artemisiifolia; Amb a), giant ragweed (Ambrosia trifida; Amb t), and western ragweed (Ambrosia psilostachya; Amb p). Varied geographic distributions of ragweed species raise questions regarding the need for ragweed species-specific allergen immunotherapy.


OBJECTIVE
To determine allergenic cross-reactivity among ragweed species by immunologic analyses of sera from subjects allergic to ragweed from North America and Europe.


METHODS
Sera were collected from 452 subjects allergic to ragweed who participated in Amb a sublingual immunotherapy tablet clinical trials. All subjects had positive skin prick test and serum IgE against Amb a. Ragweed-specific IgE (pre treatment) and IgG4 (post treatment) were measured by ImmunoCAP. IgE inhibition studies among Amb a, Amb t, and Amb p were conducted. Using pooled sera from another ragweed-allergic population, IgE inhibition studies of 7 less widespread Ambrosia species also were conducted.


RESULTS
A strong correlation between Amb a vs Amb p and Amb t serum IgE levels was observed. In the vast majority of pretreatment sera, Amb a inhibited Amb a, Amb p, and Amb t IgE reactivity by more than 90%. Strong correlations were observed between Amb a vs Amb p and Amb t post-treatment IgG4 levels. In pooled sera, Amb a extract inhibited the binding of serum IgE to all 10 ragweed species by 98%-100%.


CONCLUSIONS
In a population of subjects allergic to Amb a, substantial allergenic cross-reactivity among Amb a, Amb p, and Amb t was demonstrated. These in vitro data suggest that an Amb a-based single-species ragweed allergen immunotherapy may be therapeutically active in patients exposed to diverse ragweed pollens.


BACKGROUND
Clinicaltrials.gov, NCT00770315, NCT00783198, and NCT00330083.
ALK, Hørsholm, Denmark.
Lars H
LH
Christensen
ALK, Hørsholm, Denmark.
Henrik
H
Ipsen
Merck & Co, Inc, Kenilworth, New Jersey.
Hendrik
H
Nolte
Merck & Co, Inc, Kenilworth, New Jersey; Current affiliation: Regeneron Pharmaceuticals, Inc, Tarrytown, New York.
Jennifer
J
Maloney
National Jewish Health, Denver, Colorado.
Harold S
HS
Nelson
National Jewish Health, Denver, Colorado.
Richard
R
Weber
ALK, Hørsholm, Denmark. Electronic address: kludk@alk.net.
Kaare
K
Lund
D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology*; D031164:Ambrosia / Q000145:classification / Q000276:immunology; D052179:Antigens, Plant / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology*; D063729:Sublingual Immunotherapy; D055815:Young Adult
D000485:Allergens; D052179:Antigens, Plant; D007074:Immunoglobulin G; D010940:Plant Proteins; D007073:Immunoglobulin E