Label-Free and Real-Time Detection of Avian Influenza Using Nanowire Field Effect Transistors.
Real-time and label-free detection of antibodies from avian influenza (anti-AI) in an aqueous solution is demonstrated with the use of a nanowire field effect transistor. A real-time measurement system is constructed without leakage paths through the solution medium. The current through the nanowire changes significantly after an injection of an anti-AI solution onto the device, which was previously functionalized by the antigen of AI as a probe of anti-AI. In contrast, no significant response arises when an anti-AI solution is injected onto a non-functionalized device. Therefore, the real-time detection of specific antibody-antigen interaction of the AI is successfully implemented for a chip-based biosensor.
Jae-Hyuk
JH
Ahn
Maesoon
M
Im
Tae Jung
TJ
Park
Sang Yup
SY
Lee
Yang-Kyu
YK
Choi
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation; D001717:Birds; D003199:Computer Systems; D003217:Conductometry / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000276:immunology*; D036103:Nanotechnology / Q000295:instrumentation; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014173:Transistors, Electronic
D000914:Antibodies, Viral