Gene expression profiling of human fibrocytic myeloid-derived suppressor cells (f-MDSCs).
Myeloid-derived suppressor cells (MDSCs) have been shown to control self-reactive and anti-graft effector T-cells in autoimmunity and transplantation, but their therapeutic use is limited by their scarce availability in the peripheral blood of tumor-free donors. We isolated and characterized a novel population of myeloid suppressor cells, named fibrocytic MDSC (f-MDSC), which are differentiated from umbilical cord blood (UCB) precursors (Zoso et al., 2014). This MDSC subset promotes regulatory T-cell expansion and induces normoglycemia in a xenogeneic model of type 1 diabetes. Here we describe in details the experimental design and the bioinformatics analyses of the gene expression dataset used to investigate the molecular mechanisms at the base of MDSC tolerogenic and suppressive properties. We also provide an R code to easily access the data and perform the quality controls and basic analyses relevant to this dataset. Raw and pre-processed data are available at Gene Expression Omnibus under accession GSE52376.
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Emilia Maria Cristina
EM
Mazza
Diabetes Research Institute, University of Miami, FL, USA.
Alessia
A
Zoso
Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy ; Istituto Oncologico Veneto IOV - IRCCS, Padova, Italy.
Susanna
S
Mandruzzato
Department of Pathology, Verona University Hospital, Immunology Section, Verona, Italy.
Vincenzo
V
Bronte
Department of Microbiology and Immunology, University of Miami, Miami, FL, USA ; Diabetes Research Institute, University of Miami, FL, USA.
Paolo
P
Serafini
Diabetes Research Institute, University of Miami, FL, USA.
Luca
L
Inverardi
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Silvio
S
Bicciato
Microarrays; Myeloid cells; Transcriptional data