Dietary fiber prevents obesity-related liver lipotoxicity by modulating sterol-regulatory element binding protein pathway in C57BL/6J mice fed a high-fat/cholesterol diet.
Adequate intake of dietary fibers has proven metabolic and cardiovascular benefits, molecular mechanisms remain still limited. This study was aimed to investigate the effects of cereal dietary fiber on obesity-related liver lipotoxicity in C57BL/6J mice fed a high-fat/cholesterol (HFC) diet and underlying mechanism. Forty-eight adult male C57BL/6J mice were randomly given a reference chow diet, or a high fat/cholesterol (HFC) diet supplemented with or without oat fiber or wheat bran fiber for 24 weeks. Our results showed mice fed oat or wheat bran fiber exhibited lower weight gain, lipid profiles and insulin resistance, compared with HFC diet. The two cereal dietary fibers potently decreased protein expressions of sterol regulatory element binding protein-1 and key factors involved in lipogenesis, including fatty acid synthase and acetyl-CoA carboxylase in target tissues. At molecular level, the two cereal dietary fibers augmented protein expressions of peroxisome proliferator-activated receptor alpha and gamma, liver X receptor alpha, and ATP-binding cassette transporter A1 in target tissues. Our findings indicated that cereal dietary fiber supplementation abrogated obesity-related liver lipotoxicity and dyslipidemia in C57BL/6J mice fed a HFC diet. In addition, the efficacy of oat fiber is greater than wheat bran fiber in normalizing these metabolic disorders and pathological profiles.
Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Shufen
S
Han
Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Jun
J
Jiao
Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Wei
W
Zhang
Key Laboratory of Radiation Biology, School of Radiation Medicine and Protection, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Jiaying
J
Xu
Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Zhongxiao
Z
Wan
DSM Nutritional Products Human Nutrition and Health, Unite 5-7, Floor 8, Tower C, Parkview Green, 9 Dongdaqiao Road, Beijing 100020, China.
Weiguo
W
Zhang
DSM Nutritional Products Human Nutrition and Health, 476 Libing Road, Shanghai 201203, China.
Xiaoran
X
Gao
Department of Nutrition and Food Hygiene, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Renai Road, Suzhou City 215123, China.
Liqiang
L
Qin
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis; D000818:Animals; D002784:Cholesterol / Q000009:adverse effects* / Q000494:pharmacology; D004041:Dietary Fats; D004043:Dietary Fiber / Q000009:adverse effects* / Q000494:pharmacology; D050171:Dyslipidemias / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D054890:Fatty Acid Synthase, Type I / Q000096:biosynthesis; D007333:Insulin Resistance; D050155:Lipogenesis / Q000187:drug effects*; D000071518:Liver X Receptors; D008297:Male; D051379:Mice; D057093:Orphan Nuclear Receptors / Q000096:biosynthesis; D047493:PPAR alpha / Q000096:biosynthesis; D047495:PPAR gamma / Q000096:biosynthesis; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism*
D004041:Dietary Fats; D004043:Dietary Fiber; D000071518:Liver X Receptors; C000605213:NR1H3 protein, human; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; D047493:PPAR alpha; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D002784:Cholesterol; D054890:Fatty Acid Synthase, Type I; D000103:Acetyl-CoA Carboxylase