Resveratrol could reverse the expression of SIRT1 and MMP-1 in vitro.
Intervertebral disc degeneration is the main cause of lumbago disease, in which the extracellular matrix structure and moisture in the nucleus pulposus is lost continuously. In this study, we aimed to detect differential expression of silence mating type information regulation 2 homolog 1 (SIRT1) and matrix metalloproteinase-1 (MMP-1) in human intervertebral disc nucleus pulposus cells and to explore the effects of SIRT1 and MMP-1 on the development of the intervertebral disc degeneration. Intervertebral disc nucleus pulposus specimens from 41 patients who underwent lumbar protrusion resection at HuiZhou Municipal Central Hospital, during the period from October 2011 to December 2013, were studied in comparison with 23 control cases from patients who underwent fractured lumbar resection. In degenerated human intervertebral disc nucleus pulposus cells, the expression of SIRT1 is decreased and MMP-1 is increased compared with that of the control cells. Resveratrol could reverse these effects, thereby increasing the expression of SIRT1 (0.87 ± 0.07 vs 0.54 ± 0.04), Coll2α1 (0.90 ± 0.08 vs 0.38 ± 0.01), and aggrecan (0.69 ± 0.07 vs 0.42 ± 0.05) and decreasing the expression of MMP-1 (0.61 ± 0.03 vs 0.93 ± 0.08). These results suggest that resveratrol could possibly reverse the process of intervertebral disc degeneration and thus could be applied as a potential drug for the disease.
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
J W
JW
Wu
Department of Ophthalmology, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
J J
JJ
Wang
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
J B
JB
Chen
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
Y L
YL
Huang
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
H
H
Wang
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
G H
GH
Liu
Department of Emergency, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
L F
LF
Li
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
M
M
Kang
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
X G
XG
Wang
Department of Spine Surgery, HuiZhou Municipal Central Hospital, HuiZhou, Guangdong, China.
H H
HH
Cai
D000328:Adult; D000368:Aged; D053674:Aggrecans / Q000378:metabolism; D002478:Cells, Cultured; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D055815:Young Adult
D053674:Aggrecans; D024043:Collagen Type II; D013267:Stilbenes; D020781:Matrix Metalloproteinase 1; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol