Water revealed as molecular mirror when measuring low concentrations of sugar with near infrared light.
Near infrared spectroscopy is an overtone spectroscopy regarded as a quick and non-destructive method that provides analytical solutions for components that represent approximately 1% or more of the total mass of the investigated composite samples. Aquaphotomics offers the possibility for disentanglement of information remaining hidden in the spectra when conventional data evaluation methods are used, since this concept utilizes changes of the water structure induced by the measured solute as specific molecular vibrations at water bands. Here, near infrared technique and aquaphotomics are applied for non-destructive identification and quantification of mono- and di-saccharide solutes at 100-0.02 mM concentration that is accepted as unachievable with near infrared spectroscopy. The results presented in this study support the aquaphotomics' water molecular mirror concept that explores spectral changes related to water molecular rearrangements caused by minute changes of the solutes in the aqueous systems. The method provides quick and accurate alternative for classical analytical measurements of saccharides even at millimolar concentration levels.
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan; Institute of Food and Agricultural Product Qualification, Faculty of Agricultural and Environmental Sciences, Kaposvár University, Kaposvár, Hungary.
György
G
Bázár
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan; Department of Physics and Control, Faculty of Food Science, Corvinus University of Budapest, Budapest, Hungary.
Zoltan
Z
Kovacs
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Mariko
M
Tanaka
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Akane
A
Furukawa
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Airi
A
Nagai
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Manami
M
Osawa
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Yukari
Y
Itakura
Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, Japan.
Hiroshi
H
Sugiyama
Biomeasurement Technology Laboratory, Graduate School of Agricultural Science, Kobe University, Kobe, Japan. Electronic address: rtsen@kobe-u.ac.jp.
Roumiana
R
Tsenkova
D004187:Disaccharides / Q000032:analysis*; D007259:Infrared Rays; D009005:Monosaccharides / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared; D014867:Water / Q000737:chemistry*
D004187:Disaccharides; D009005:Monosaccharides; D014867:Water
Aquaphotomics; Low concentrations of sugar; Molecular vibrations at water bands; Near infrared spectroscopy