G-Tetraplex-Induced FRET within Telomeric Repeat Sequences Using (Py) A-(Per) A as Energy Donor-Acceptor Pair.
G-tetraplex induced fluorescence resonance energy transfer (FRET) within telomeric repeat sequences has been studied using a nucleoside-tethered FRET pair embedded in the human telomeric G-quadruplex forming sequence (5'-A GGG TT(Py) A GGG TT(Per) A GGG TTA GGG-3', Py=pyrene, Per=perylene). Conformational change from a single strand to an anti-parallel G-quadruplex leads to FRET from energy donor ((Py) A) to acceptor ((Per) A). The distance between the FRET donor/acceptor partners was controlled by changing the number of G-quartet spacer units. The FRET efficiency decreases with increase in G-quartet units. Overall findings indicate that this could be further used for the development of FRET-based sensing and measurement techniques.
Department of Chemistry, Pohang University of Science and Technology, Pohang, 790784, South Korea. kundurajen@postech.ac.kr.|Department of Chemistry and Biochemistry, University of Colorado, Boulder, 80303, USA. kundurajen@postech.ac.kr.
Rajen
R
Kundu
D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques; D011721:Pyrenes / Q000737:chemistry; D016615:Telomere / Q000737:chemistry*
D011721:Pyrenes; D004247:DNA
FRET; G-quadruplex; pyrene-perylene; telomere; tetraplex