Complete genome sequence of a distinct calla lily chlorotic spot virus isolated in mainland China.
The first complete genome sequence of calla lily chlorotic spot virus (CCSV) from Lijiang in northwestern Yunnan Province was obtained using RT-PCR with designed primers. The genome of CCSV isolate LJ-1-Yunnan is tripartite. The small (S) RNA is 3182 nucleotides (nt) in length and encodes a nonstructural protein (NSs, 1383 nt) and a nuclear nucleocapsid (N, 834 nt), separated by an 836-nt intergenic region (IGR). The medium (M) RNA is 4749 nt in length and encodes a nonstructural movement protein (NSm, 930 nt) and a glycoprotein (GnGc, 3,372 nt), also separated by a 349-nt IGR. The large (L) RNA is 8912 nt in length and encodes a predicted RNA-dependent RNA polymerase (RdRp, 8652 nt). The nucleotide sequences of the three viral RNA segments are 92-94 % identical to the published CCSV genome sequence, and the amino acid sequences of the encoded proteins are 96-98 % identical. However, the IGRs of the S and M RNAs are less similar, with 86 and 72 % identity, respectively. Genome sequence comparisons and phylogenetic analysis indicate that the Lijiang CCSV isolate is a unique tospovirus isolate that differs from CCSV isolates in other geographic regions.
College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
Ye
Y
Xu
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Si-Bo
SB
Wang
Yunnan Vocational and Technical College of Agriculture, Kunming, 650201, China.
Yong-Zhong
YZ
Li
College of Life Science and Technology, Honghe University, Mengzi, 661199, China.
Hong-Zheng
HZ
Tao
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Ya-Ning
YN
Huang
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Bao-Wei
BW
Wu
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Yu-Mei
YM
Dong
College of Tropical Agriculture and Human Resources, University of Hawaii, Honolulu, HI, 96822, USA.
John
J
Hu
Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China. liuyating66@163.com.
Ya-Ting
YT
Liu
D001483:Base Sequence; D002681:China; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D014026:Nicotiana / Q000821:virology*; D017865:Tospovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins