Are RIG-1 and MDA5 Expressions Associated with Chronic HBV Infection?
Melanoma differentiation-associated protein 5 (MDA5) and retinoic acid-inducible gene 1 (RIG-1) as the pattern recognition receptors play important roles in viral mRNA recognition. Chronic HBV-infected (CHB) patients are unable to properly respond to hepatitis B virus (HBV). Therefore, the aim of the present study was to evaluate the mRNA levels of MDA5 and RIG-1 in the peripheral blood immune cells of CHB patients in comparison to healthy controls. In this cross-sectional study, the mRNA levels of MDA5 and RIG-1 were examined in 60 CHB patients and 60 healthy controls using the real-time polymerase chain reaction (PCR) technique. Our results showed that mRNA levels of MDA5 and RIG-1 were significantly decreased and increased, respectively, in CHB patients when compared to healthy controls. Our results also revealed that mRNA levels of MDA5 and RIG-1 were not altered among CHB patients with various states of e-antigen of hepatitis B and HBV-DNA viral loads. According to the results presented here, it may be concluded that downregulation of MDA5 may be a responsible mechanism from several reasons, which leads to HBV persistence in CHB patients.
1 Geriatric Care Research Center, Rafsanjan University of Medical Sciences , Rafsanjan, Iran .
Maryam
M
Ebrahim
2 Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences , Rafsanjan, Iran .
Vahid
V
Mirzaei
3 Department of Psychiatry, Research Center for Addiction and Behavioral Sciences, Shahid Sadoughi University of Medical Sciences , Rafsanjan, Iran .
Reza
R
Bidaki
2 Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences , Rafsanjan, Iran .
Ziba
Z
Shabani
4 Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences , Kerman, Iran .
Hamid
H
Daneshvar
4 Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences , Kerman, Iran .
Masoud
M
Karimi-Googheri
4 Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences , Kerman, Iran .
Mehdi
M
Khaleghinia
5 Rafsanjan Center of Iranian Blood Transfusion Organization , Rafsanjan, Iran .
Mohammad Reza
MR
Afrooz
6 Khalil Abad Health Center, Mashhad University of Medical Sciences , Mashhad, Iran .
Yaser
Y
Yousefpoor
2 Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences , Rafsanjan, Iran .
Mohammad Kazemi
MK
Arababadi
D003430:Cross-Sectional Studies; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000473:pathology*; D006801:Humans; D000072640:Interferon-Induced Helicase, IFIH1; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011971:Receptors, Immunologic; D019562:Viral Load
D006513:Hepatitis B e Antigens; D012333:RNA, Messenger; D011971:Receptors, Immunologic; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D000072640:Interferon-Induced Helicase, IFIH1