The MRI marker gene MagA attenuates the oxidative damage induced by iron overload in transgenic mice.
We aimed to create transgenic (Tg) mice engineered for magnetic resonance imaging (MRI). To ascertain if MagA expression contributes to oxidative stress and iron metabolism, we report the generation of Tg mice in which ubiquitous expression of MagA can be detected by MRI in vivo. Expression of MagA in diverse tissues of Tg mice was assessed, and iron accumulation and deposition of nanoparticles in tissues were analyzed. Levels of antioxidant enzymes, lipid peroxidation and cytokine production were determined, and iron metabolism-related proteins were also detected. MagA Tg showed no apparent pathologic symptoms and no histologic changes compared with wild-type (WT) mice. Overexpression of MagA resulted in specific alterations of the transverse relaxation rate (R2) of water. Transgene-dependent changes in R2 were detectable by MRI in iron-overloaded mice. We also evaluated antioxidant abilities between WT and Tg groups or two iron-overloaded groups. Together with the data of cytokines and iron metabolism-related proteins, we inferred that MagA could regulate nanoparticle production and thus attenuate the oxidative damage induced by iron overload. The novel MagA Tg mouse, which expresses an MRI reporter in many tissues, would be a valuable model of MagA molecular imaging in which to study diseases related to iron metabolism.
a State Key Laboratory of Oncology in South China; Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine , Guangzhou , People's Republic of China .|b Department of Pathology , Guangzhou Medical University , Guangzhou , People's Republic of China , and.
Xiaoying
X
Guan
a State Key Laboratory of Oncology in South China; Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine , Guangzhou , People's Republic of China .
Xinhua
X
Jiang
a State Key Laboratory of Oncology in South China; Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine , Guangzhou , People's Republic of China .
Chuan
C
Yang
c Department of Biomedical Engineering , Guangzhou Medical University , Guangzhou , People's Republic of China.
Xiumei
X
Tian
a State Key Laboratory of Oncology in South China; Sun Yat-sen University Cancer Center, Collaborative Innovation Center for Cancer Medicine , Guangzhou , People's Republic of China .
Li
L
Li
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D027682:Cation Transport Proteins / Q000235:genetics*; D005260:Female; D017930:Genes, Reporter; D007501:Iron / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D019190:Iron Overload / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008822:Mice, Transgenic; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000235:genetics; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution
D001426:Bacterial Proteins; D015415:Biomarkers; D027682:Cation Transport Proteins; C097085:MagA protein, Magnetospirillum; D007501:Iron
Iron overload; MagA; magnetic resonance imaging; oxidative stress; transgenic mouse