Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation.
The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12 h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation.
Department of Pathophysiology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Marija
M
Stankovic
Laboratory for Cellular Physiology, Institute of Medical Physiology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Igor
I
Pantic
Department of Pathophysiology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Silvio R
SR
De Luka
Institute of Histology and Embryology "Aleksandar Đ. Kostić", School of Medicine, University of Belgrade, Belgrade, Serbia.
Nela
N
Puskas
Institute of Histology and Embryology "Aleksandar Đ. Kostić", School of Medicine, University of Belgrade, Belgrade, Serbia.
Ivan
I
Zaletel
General and Oral Histology and Embryology, Faculty of Dentistry, University of Belgrade, Belgrade, Serbia.
Sanja
S
Milutinovic-Smiljanic
Institute of Histology and Embryology "Aleksandar Đ. Kostić", School of Medicine, University of Belgrade, Belgrade, Serbia.
Senka
S
Pantic
Department of Pathophysiology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Alexander M
AM
Trbovich
D000818:Animals; D017709:Fractals; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000473:pathology* / Q000648:ultrastructure*; D011897:Random Allocation; D014425:Turpentine
D014425:Turpentine
Acute inflammation; angular second moment; correlation; fractal dimension