Further evaluation of an ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease virus.
An ELISA kit for detection of antibodies to a nonstructural protein of foot-and-mouth disease (FMDV) was further evaluated using sequentially collected serum samples of experimentally infected animals, because the sensitivity of the kit used in a previous study was significantly low in field animals. The kit fully detected antibodies in infected animals without vaccination; however, the first detections of antibodies by the kit were later than those by the liquid-phase blocking ELISA that is used for serological surveillance in the aftermath of outbreaks in Japan, for detection of antibodies to structural proteins of FMDV. Additionally, although the kit effectively detected antibodies in infected cattle with vaccination, there were several infected pigs with vaccination for which the kit did not detect antibodies during the experimental period. Taken together, the kit may not be suitable for serological surveillance after an FMD outbreak either with or without emergency vaccination in FMD-free countries.
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187-0022, Japan.
Katsuhiko
K
Fukai
Tatsuya
T
Nishi
Kazuki
K
Morioka
Manabu
M
Yamada
Kazuo
K
Yoshida
Rie
R
Kitano
Reiko
R
Yamazoe
Toru
T
Kanno
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D062665:Epidemiological Monitoring / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D015511:Goat Diseases / Q000276:immunology / Q000821:virology; D006041:Goats; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines