Collagen/Beta-Tricalcium Phosphate Based Synthetic Bone Grafts via Dehydrothermal Processing.
Millions of patients worldwide remain inadequately treated for bone defects related to factors such as disease or trauma. The drawbacks of metallic implant and autograft/allograft use have steered therapeutic approaches towards tissue engineering solutions involving tissue regeneration scaffolds. This study proposes a composite scaffold with properties tailored to address the macro- and microenvironmental conditions deemed necessary for successful regeneration of bone in defect areas. The biodegradable scaffold composed of porous beta-tricalcium phosphate particles and collagen type I fibers is prepared from a mixture of collagen type-I and β-tricalcium phosphate (β-TCP) particles via lyophilization, followed by dehydrothermal (DHT) processing. The effects of both sterilization via gamma radiation and the use of DHT processing to achieve cross-linking were investigated. The impact of the chosen fabrication methods on scaffold microstructure and β-TCP particle-collagen fiber combinations were analyzed using X-ray diffractometry (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and microcomputerized tomography (µ-CT). Electron spinning resonance (ESR) analysis was used to investigate free radicals formation following sterilization. Results revealed that the highly porous (65% porosity at an average of 100 µm pore size), mechanically adequate, and biocompatible scaffolds can be utilized for bone defect repairs.
Institute of Science, Bioengineering Division, Hacettepe University, 06800 Ankara, Turkey ; Faculty of Engineering and Architecture, Genetics and Bioengineering Department, Kastamonu University, 37150 Kastamonu, Turkey.
Burcu
B
Sarikaya
BMT Calsis Health Technologies Co., 06980 Ankara, Turkey ; Environmental Engineering Department & Bioengineering Division and Centre for Bioengineering, Hacettepe University, 06800 Ankara, Turkey.
Halil Murat
HM
Aydin
D001696:Biomechanical Phenomena; D018786:Bone Substitutes / Q000737:chemistry*; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D024042:Collagen Type I / Q000737:chemistry*; D003432:Cross-Linking Reagents; D005612:Freeze Drying; D006801:Humans; D008422:Materials Testing; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
D018786:Bone Substitutes; D002130:Calcium Phosphates; D024042:Collagen Type I; D003432:Cross-Linking Reagents; C485817:beta-tricalcium phosphate