A Monolithic Hybrid Cellulose-2.5-Acetate/Polymer Bioreactor for Biocatalysis under Continuous Liquid-Liquid Conditions Using a Supported Ionic Liquid Phase.
Mesoporous monolithic hybrid cellulose-2.5-acetate (CA)/polymer supports were prepared under solvent-induced phase separation conditions using cellulose-2.5-acetate microbeads 8-14 μm in diameter, 1,1,1-tris(hydroxymethyl)propane and 4,4'-methylenebis(phenylisocyanate) as monomers as well as THF and n-heptane as porogenic solvents. 4-(Dimethylamino)pyridine and dibutyltin dilaurate (DBTDL), respectively, were used as catalysts. Monolithic hybrid supports were used in transesterification reactions of vinyl butyrate with 1-butanol under continuous, supported ionic liquid-liquid conditions with Candida antarctica lipase B (CALB) and octylmethylimidazolium tetrafluoroborate ([OMIM(+) ][BF4 (-) ]) immobilized within the CA beads inside the polymeric monolithic framework and methyl tert-butyl ether (MTBE) as the continuous phase. The new hybrid bioreactors were successfully used in dimensions up to 2×30 cm (V=94 mL). Under continuous biphasic liquid-liquid conditions a constant conversion up to 96 % was achieved over a period of 18 days, resulting in a productivity of 58 μmol mg(-1) (CALB) min(-1) . This translates into an unprecedented turnover number (TON) of 3.9×10(7) within two weeks, which is much higher than the one obtained under standard biphasic conditions using [OMIM(+) ][BF4 (-) ]/MTBE (TON=2.7×10(6) ). The continuous liquid-liquid setup based on a hybrid reactor presented here is strongly believed to be applicable to many other enzyme-catalyzed reactions.
Lehrstuhl für Makromolekulare Stoffe und Faserchemie, Institut für Polymerchemie, Universität Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart (Germany), Fax: (+49)-711-685-64050.
Bernhard
B
Sandig
Lehrstuhl für Makromolekulare Stoffe und Faserchemie, Institut für Polymerchemie, Universität Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart (Germany), Fax: (+49)-711-685-64050.
Lukas
L
Michalek
Institut für Technische Biochemie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart (Germany).
Sandra
S
Vlahovic
Institut für Technische Biochemie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart (Germany).
Mihaela
M
Antonovici
Institut für Technische Biochemie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart (Germany).
Bernhard
B
Hauer
Lehrstuhl für Makromolekulare Stoffe und Faserchemie, Institut für Polymerchemie, Universität Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart (Germany), Fax: (+49)-711-685-64050. michael.buchmeiser@ipoc.uni-stuttgart.de.|Institut für Textilchemie und Chemiefasern (ITCF) Denkendorf, Körschtalstr. 26, 73770 Denkendorf (Germany). michael.buchmeiser@ipoc.uni-stuttgart.de.
Michael R
MR
Buchmeiser
D000631:Aminopyridines / Q000737:chemistry*; D055162:Biocatalysis; D002175:Candida / Q000737:chemistry* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D052578:Ionic Liquids / Q000737:chemistry*; D007477:Ions / Q000737:chemistry*; D017953:Isocyanates / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D009947:Organotin Compounds / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D001559:Benzhydryl Compounds
D000631:Aminopyridines; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D052578:Ionic Liquids; D007477:Ions; D017953:Isocyanates; D008049:Lipase; D009947:Organotin Compounds; D011409:Propylene Glycols; D012997:Solvents; C000607852:4-(dimethylamino)pyridine; C018163:trimethylolpropane; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds; C431349:lipase B, Candida antarctica; C010409:dibutyltin dilaurate
biocatalysis; biphasic catalysis; enzymes; ionic liquids; transesterification