A new triterpenoid saponin from Clinopodium chinense (Benth.) O. Kuntze.
A new triterpene saponin, 3β,16β,23α,28β,30β-pentahydroxyl-olean-11,13(18)-dien-3β-yl-[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranoside, was named Clinoposaponin D (1), together with six known triterpene saponins, buddlejasaponin IVb (2), buddlejasaponin IVa (3), buddlejasaponin IV (4), clinopodisides D (5), 11α,16β,23,28-Tetrahydroxyolean-12-en-3β-yl-[β-D-glucopyranosyl-(1→2)]-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranoside (6) and prosaikogenin A (7), and two known triterpenes, saikogenin A (8) and saikogenin F (9) were isolated from Clinopodium chinense (Benth.) O. Kuntze. Their structures were elucidated on the basis of 1D, 2D NMR and MS analysis. Meanwhile, the effects of all compounds on rabbit platelet aggregation and thrombin time (TT) were investigated in vitro. Compounds 4 and 7 had significant promoting effects on platelet aggregation with EC50 value at 53.4 and 12.2 μM, respectively. In addition, the highest concentration (200 μM) of compounds 2 and 9 shortened TT by 20.6 and 25.1%, respectively.
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.|b Guangzhou Baiyun Shan Ming Xing Pharmaceutical Co. Ltd. , Guangzhou , China.
Biao
B
Zeng
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Guang-Da
GD
Liu
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Bao-Bao
BB
Zhang
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Shan-shan
SS
Wang
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Rui
R
Ma
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Bei-Shan
BS
Zhong
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Bai-qiu
BQ
He
c Nanjing Sanhome Pharmaceutical Co. Ltd. , Nanjing , China.
Yan
Y
Liang
a School of Traditional Chinese Pharmacy , China Pharmaceutical University , Nanjing , China.
Fei-Hua
FH
Wu
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D066298:In Vitro Techniques; D019686:Lamiaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000032:analysis; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012502:Sapogenins / Q000032:analysis; D012503:Saponins / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013918:Thrombin Time; D014315:Triterpenes / Q000032:analysis*
D012502:Sapogenins; D012503:Saponins; D014315:Triterpenes; C104869:buddlejasaponin; C000611781:clinoposaponin D; C113998:prosaikogenin A; C439329:saikogenin F; C009198:saikogenin A; D009828:Oleanolic Acid
Clinopodium chinense (Benth.) O. Kuntze; platelet aggregation; thrombin time; triterpene saponin