Investigations on microbial diversity of Jakrem hot spring, Meghalaya, India using cultivation-independent approach.
Jakrem hot water spring is located in the West Khasi Hill District of the state of Meghalaya, and is one of the most popular hot springs of the state. There is a populist belief among the inhabitants and people that the hot spring water has got curative properties against various skin ailments. This is the first report on V3 hyper-variable region of 16S rDNA metagenome sequence employing Illumina platform to profile the microbial community of this less known hot spring from Meghalaya, India. Metagenome comprised of 10, 74,120 raw sequences with a sequence length of 151 bp and 56.35% G + C content. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP056897. A total of 8, 77, 364 pre-processed reads were clustered into 694 OTUs (operational taxonomical units) comprising of 14 bacterial phyla including unknown phylum demonstrating 49 families. Hot spring bacterial community is dominated by Firmicutes (61.60%), Chloroflexi (21.37%), Cyanobacteria (12.96%) and unclassified bacteria (1.2%) respectively.
Department of Zoology, Kumaun University, Nainital 263002, Uttarakhand, India.
Amrita Kumari
AK
Panda
Department of Zoology, Kumaun University, Nainital 263002, Uttarakhand, India.
Satpal Singh
SS
Bisht
Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.
Nachimuthu Senthil
NS
Kumar
Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.
Surajit
S
De Mandal
Alkaline hot spring; Firmicutes; Illumina sequencing; Metagenome; Microbial diversity