Trichloroacetic acid treatment as a tricky way for rapid purification of 1N/4R tau protein.
Tau protein consists of six different isoforms and each one has particular physiological roles. In order to analyze the specific function of each single isoforms, large quantity of highly purified tau isoforms is essential. Many studies have been done to purify tau isoforms by heat treatment, followed by perchloric acid and glycerol precipitation. We found out that 1N/4R tau is soluble in glycerol, that is why mentioned methods do not work for purifying this isoform. In this study, large amounts of active and highly purified (97%) 1N/4R tau protein has been prepared by utilization of trichloroacetic acid as precipitating agent.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Kazem
K
Asadollahi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Saharnaz
S
Rafiee
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Electronic address: ghriazi@ut.ac.ir.
Gholam Hossein
GH
Riazi
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Shahriar
S
Pooyan
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Ali
A
Afrasiabi
D005591:Chemical Fractionation / Q000379:methods*; D011232:Chemical Precipitation; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014238:Trichloroacetic Acid / Q000737:chemistry*; D016875:tau Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D020033:Protein Isoforms; D016875:tau Proteins; D014238:Trichloroacetic Acid
4R/1N tau; Differential precipitation; Heat treatment; Perchloric acid; Protein purification; Trichloroacetic acid