Adjuvant treatment with tumor-targeting Salmonella typhimurium A1-R reduces recurrence and increases survival after liver metastasis resection in an orthotopic nude mouse model.
Colon cancer liver metastasis is often the lethal aspect of this disease. Well-isolated metastases are candidates for surgical resection, but recurrence is common. Better adjuvant treatment is therefore needed to reduce or prevent recurrence. In the present study, HT-29 human colon cancer cells expressing red fluorescent protein (RFP) were used to establish liver metastases in nude mice. Mice with a single liver metastasis were randomized into bright-light surgery (BLS) or the combination of BLS and adjuvant treatment with tumor-targeting S. typhimurium A1-R. Residual tumor fluorescence after BLS was clearly visualized at high magnification by fluorescence imaging. Adjuvant treatment with S. typhimurium A1-R was highly effective to increase survival and disease-free survival after BLS of liver metastasis. The results suggest the future clinical potential of adjuvant S. typhimurium A1-R treatment after liver metastasis resection.
AntiCancer, Inc., San Diego, California, USA.|Department of Surgery, University of California, San Diego, California, USA.|Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Takashi
T
Murakami
AntiCancer, Inc., San Diego, California, USA.|Department of Surgery, University of California, San Diego, California, USA.|Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Yukihiko
Y
Hiroshima
AntiCancer, Inc., San Diego, California, USA.
Ming
M
Zhao
AntiCancer, Inc., San Diego, California, USA.
Yong
Y
Zhang
Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Takashi
T
Chishima
Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Kuniya
K
Tanaka
Department of Surgery, University of California, San Diego, California, USA.
Michael
M
Bouvet
Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
Itaru
I
Endo
AntiCancer, Inc., San Diego, California, USA.|Department of Surgery, University of California, San Diego, California, USA.
Robert M
RM
Hoffman
D000818:Animals; D001691:Biological Therapy / Q000379:methods*; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003131:Combined Modality Therapy; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D019073:HT29 Cells; D006498:Hepatectomy; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000556:secondary / Q000628:therapy*; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008819:Mice, Nude; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D013997:Time Factors; D014162:Transfection; D023041:Xenograft Model Antitumor Assays; D000097573:Red Fluorescent Protein
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
RFP; colon cancer; liver metastasis; nude mice; orthotopic models