Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans.
Many genes play essential roles in development and fertility; their disruption leads to growth arrest or sterility. Genetic balancers have been widely used to study essential genes in many organisms. However, it is technically challenging and laborious to generate and maintain the loss-of-function mutations of essential genes. The CRISPR/Cas9 technology has been successfully applied for gene editing and chromosome engineering. Here, we have developed a method to induce chromosomal translocations and produce genetic balancers using the CRISPR/Cas9 technology and have applied this approach to edit essential genes in Caenorhabditis elegans. The co-injection of dual small guide RNA targeting genes on different chromosomes resulted in reciprocal translocation between nonhomologous chromosomes. These animals with chromosomal translocations were subsequently crossed with animals that contain normal sets of chromosomes. The F1 progeny were subjected to a second round of Cas9-mediated gene editing. Through this method, we successfully produced nematode strains with specified chromosomal translocations and generated a number of loss-of-function alleles of two essential genes (csr-1 and mes-6). Therefore, our method provides an easy and efficient approach to generate and maintain loss-of-function alleles of essential genes with detailed genetic background information.
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Xiangyang
X
Chen
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Mu
M
Li
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China sguang@ustc.edu.cn fengxz@ustc.edu.cn.
Xuezhu
X
Feng
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China sguang@ustc.edu.cn fengxz@ustc.edu.cn.
Shouhong
S
Guang
D000483:Alleles; D000818:Animals; D064113:CRISPR-Cas Systems; D017173:Caenorhabditis elegans / Q000235:genetics*; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods*; D020043:Genes, Essential; D017238:Genes, Helminth; D008297:Male; D014178:Translocation, Genetic
CRISPR/Cas9; balancer; chromosomal translocation; essential gene knockout