Surface functionalization allowing repetitive use of optical sensors for real-time detection of antibody-bacteria interaction.
In this study, sensor surface functionalization allowing the repetitive use of a sensing device was evaluated for antibody-based detection of living bacteria using an optical planar Bragg grating sensor. To achieve regenerable immobilization of bacteria specific antibodies, the heterobifunctional cross-linker N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) was linked to an aminosilanized sensor surface and subsequently reduced to expose sulfhydryl groups enabling the covalent conjugation of SPDP-activated antibodies via disulfide bonds. The immobilization of a capture antibody specific for Staphylococcus aureus on the sensor surface as well as specific binding of S. aureus could be monitored, highlighting the applicability of optical sensors for the specific detection of large biological structures. Reusability of bacteria saturated sensors was successfully demonstrated by cleaving the antibody along with bound bacteria through reduction of disulfide bonds and subsequent re-functionalization with activated antibody, resulting in comparable sensitivity towards S. aureus.
Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, 97074, Wuerzburg, Germany.
Marika
M
Kutscher
Applied Laser and Photonics Group, University of Applied Sciences Aschaffenburg, Wuerzburger Straße 45, 63743, Aschaffenburg, Germany.
Manuel
M
Rosenberger
Institute of Microwaves and Photonics, University of Erlangen-Nuernberg, Cauerstraße 9, 91058, Erlangen, Germany.
Bernhard
B
Schmauss
Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, 97074, Wuerzburg, Germany.
Lorenz
L
Meinel
Department of General, Visceral, Vascular and Paediatric Surgery, University Clinic of Wuerzburg, Oberduerrbacher Str. 6, 97080, Wuerzburg, Germany.
Udo
U
Lorenz
Institute for Molecular Infection Biology, University of Wuerzburg, Josef-Schneider-Str. 2, Bau D15, 97080, Wuerzburg, Germany.
Knut
K
Ohlsen
Applied Laser and Photonics Group, University of Applied Sciences Aschaffenburg, Wuerzburger Straße 45, 63743, Aschaffenburg, Germany. ralf.hellmann@h-ab.de.
Ralf
R
Hellmann
Institute for Pharmacy and Food Chemistry, University of Wuerzburg, Am Hubland, 97074, Wuerzburg, Germany. oliver.germershaus@fhnw.ch.|Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gruendenstrasse 40, 4132, Muttenz, Switzerland. oliver.germershaus@fhnw.ch.
Oliver
O
Germershaus
D000907:Antibodies, Bacterial / Q000737:chemistry*; D055910:Antibodies, Immobilized; D015374:Biosensing Techniques; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized
Planar Bragg grating sensor; S. aureus; antibody; biosensor; surface functionalization