[Influence of Different Type of Surfactant on Bacteriolytic Activity of Lysozyme].
The influence ofvarious surfactants (anionic sodium dodecyl sulfate, SDS, cationic dodecyltrimethylarnmonium bromide, DTAB, and zwitterionic cocoamidopropylbetaine, CAPB) on the activity of the chicken egg lysozyme is investigated. Lysis of Gram-positive bacteria by the enzyme was carried out at pH 7.2 and ionic strength of 0.15 M. It was found that at low SDS and DTAB concentrations (less than 1 x 10(-5) M) the bacteriolytic activity increases by 30-140%. At higher concentrations (1 x 10(-5) - 1 x 10(4) M) the activity returns to the level observed in the absence of the surfactants. The elevated activity correlated with the formation of hydrophobic lysozyme-surfactant complexes. Introduction of CAPB at concentrations above 1 x 10(-5) M sig, nificantly diminished the bacteriolytic activity due to CAPB induced aggregation of lysozyme.
R A
RA
Ivanov
O A
OA
Soboleva
S A
SA
Smirnov
P A
PA
Levashov
D000818:Animals; D001433:Bacteriolysis / Q000187:drug effects*; D001622:Betaine / Q000031:analogs & derivatives / Q000494:pharmacology; D002645:Chickens; D016982:Micrococcus luteus / Q000187:drug effects*; D009113:Muramidase / Q000187:drug effects / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013499:Surface Properties
D000644:Quaternary Ammonium Compounds; C503172:cocoamidopropylbetaine; C013912:decyltrimethylammonium; D012967:Sodium Dodecyl Sulfate; D001622:Betaine; D009113:Muramidase