In vivo assay to monitor flavonoid uptake across plant cell membranes.
Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2-aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non-pigmented Vitis vinifera cell cultures.
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Antonio
A
Filippi
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Elisa
E
Petrussa
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Carlo
C
Peresson
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Alberto
A
Bertolini
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Angelo
A
Vianello
Department of Agricultural and Environmental Sciences, Plant Biology Unit, via delle Scienze 91, I-33100 Udine, Italy.
Enrico
E
Braidot
2-Aminoethoxydiphenyl borate; DPBA, 2-aminoethoxydiphenyl borate; Flavonoid transport; Grape cell cultures; MS, Murashige and Skoog; NPC, non-pigmented cell; PFA, paraformaldehyde; Paraformaldehyde; QC, quercetin; Quercetin