Pulsed Direct Current Electrospray: Enabling Systematic Analysis of Small Volume Sample by Boosting Sample Economy.
We had developed pulsed direct current electrospray ionization mass spectrometry (pulsed-dc-ESI-MS) for systematically profiling and determining components in small volume sample. Pulsed-dc-ESI utilized constant high voltage to induce the generation of single polarity pulsed electrospray remotely. This method had significantly boosted the sample economy, so as to obtain several minutes MS signal duration from merely picoliter volume sample. The elongated MS signal duration enable us to collect abundant MS(2) information on interested components in a small volume sample for systematical analysis. This method had been successfully applied for single cell metabolomics analysis. We had obtained 2-D profile of metabolites (including exact mass and MS(2) data) from single plant and mammalian cell, concerning 1034 components and 656 components for Allium cepa and HeLa cells, respectively. Further identification had found 162 compounds and 28 different modification groups of 141 saccharides in a single Allium cepa cell, indicating pulsed-dc-ESI a powerful tool for small volume sample systematical analysis.
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Zhenwei
Z
Wei
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Xingchuang
X
Xiong
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Chengan
C
Guo
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Xingyu
X
Si
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Yaoyao
Y
Zhao
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Muyi
M
He
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Chengdui
C
Yang
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Wei
W
Xu
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Fei
F
Tang
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Xiang
X
Fang
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Sichun
S
Zhang
Beijing Key Laboratory for Microanalytical Methods, Instrumentation, Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
Xinrong
X
Zhang
D006367:HeLa Cells; D006801:Humans; D055432:Metabolomics / Q000191:economics / Q000379:methods; D019697:Onions / Q000737:chemistry* / Q000378:metabolism; D009930:Organic Chemicals / Q000032:analysis* / Q000191:economics / Q000378:metabolism; D059010:Single-Cell Analysis / Q000191:economics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000191:economics / Q000379:methods*
D009930:Organic Chemicals