Evaluation of the protective effect of chemical additives in the oxidation of phenolic compounds catalysed by peroxidase.
The use of oxidoredutive enzymes in removing organic pollutants has been the subject of much research. The oxidation of phenolic compounds in the presence of chemical additives has been the focus of this study. In this investigation, the influence of the additives polyethylene glycol and Triton X-100 was evaluated in the phenol oxidation, caffeic acid, chlorogenic acid and total phenolic compounds present in coffee processing wastewater (CPW) at different pH values, performed by turnip peroxidase and peroxidase extracted from soybean seed hulls. The influence of these additives was observed only in the oxidation of phenol and caffeic acid. In the oxidation of other studied phenolic compounds, the percentage of oxidation remained unchanged in the presence of these chemical additives. In the oxidation of CPW in the presence of additives, no change in the oxidation of phenolic compounds was observed. Although several studies show the importance of evaluating the influence of additives on the behaviour of enzymes, this study found a positive response from the economic point of view for the treatment of real wastewater, since the addition of these substances showed no influence on the oxidation of phenolic compounds, which makes the process less costly.
a Departamento de Química , Universidade Federal de Lavras , Lavras , Brazil.
Juliana Arriel
JA
Torres
a Departamento de Química , Universidade Federal de Lavras , Lavras , Brazil.
Pricila Maria Batista
PM
Chagas
b Departamento de Química , Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
Maria Cristina
MC
Silva
a Departamento de Química , Universidade Federal de Lavras , Lavras , Brazil.
Custódio Donizete
CD
Dos Santos
a Departamento de Química , Universidade Federal de Lavras , Lavras , Brazil.
Angelita Duarte
AD
Corrêa
D029688:Brassica napus / Q000201:enzymology*; D002109:Caffeic Acids / Q000302:isolation & purification / Q000378:metabolism; D002726:Chlorogenic Acid / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability / Q000187:drug effects; D007220:Industrial Waste / Q000032:analysis; D017830:Octoxynol / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000302:isolation & purification / Q000378:metabolism*; D010636:Phenols / Q000302:isolation & purification* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism; D020011:Protective Agents / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000032:analysis*; D018508:Water Purification / Q000379:methods
D002109:Caffeic Acids; D002726:Chlorogenic Acid; D007220:Industrial Waste; D017830:Octoxynol; D010544:Peroxidases; D010636:Phenols; D011092:Polyethylene Glycols; D020011:Protective Agents; D062065:Wastewater; C040048:caffeic acid
Biocatalysis; Triton X-100; peroxidases; phenolic compounds; polyethylene glycol; wastewater