Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma.
OBJECTIVE
The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.


METHODS
The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software.


RESULTS
The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma.


CONCLUSIONS
The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation.
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan; Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan. Electronic address: sohba@nagasaki-u.ac.jp.
Seigo
S
Ohba
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Yoshinori
Y
Sumita
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Mayumi
M
Umebayashi
Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Hitoshi
H
Yoshimura
Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Hisato
H
Yoshida
Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Shinpei
S
Matsuda
Division of Nephrology, Department of Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Hideki
H
Kimura
Department of Regenerative Oral Surgery, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Izumi
I
Asahina
Division of Dentistry and Oral Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Japan.
Kazuo
K
Sano
D000818:Animals; D001769:Blood; D018786:Bone Substitutes / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010012:Osteogenesis / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D012886:Skull / Q000601:surgery*; D012965:Sodium Chloride / Q000494:pharmacology
D018786:Bone Substitutes; C000611760:ReFit bone substitute; D012965:Sodium Chloride; D006820:Hyaluronic Acid; D003094:Collagen; D017886:Durapatite
Bone formation; Hydroxyapatite/collagen composite; Onlay bone graft; Platelet-rich plasma (PRP); Total blood