Covalent bonding of YIGSR and RGD to PEDOT/PSS/MWCNT-COOH composite material to improve the neural interface.
The development of coating materials for neural interfaces has been a pursued to improve the electrical, mechanical and biological performances. For these goals, a bioactive coating was developed in this work featuring a poly(3,4-ethylenedioxythiophene) (PEDOT)/carbon nanotube (CNT) composite and covalently bonded YIGSR and RGD. Its biological effect and electrical characteristics were assessed in vivo on microwire arrays (MWA). The coated electrodes exhibited a significantly higher charge storage capacity (CSC) and lower electrochemical impedance at 1 kHz which are desired to improve the stimulating and recording performances, respectively. Acute neural recording experiments revealed that coated MWA possess a higher signal/noise ratio capturing spikes undetected by uncoated electrodes. Moreover, coated MWA possessed more active sites and single units, and the noise floor of coated electrodes was lower than that of uncoated electrodes. There is little information in the literature concerning the chronic performance of bioactively modified neural interfaces in vivo. Therefore in this work, chronic in vivo tests were conducted and the PEDOT/PSS/MWCNT-polypeptide coated arrays exhibited excellent performances with the highest mean maximal amplitude from day 4 to day 12 during which the acute response severely compromised the performance of the electrodes. In brief, we developed a simple method of covalently bonding YIGSR and RGD to a PEDOT/PSS/MWCNT-COOH composite improving both the biocompatibility and electrical performance of the neural interface. Our findings suggest that YIGSR and RGD modified PEDOT/PSS/MWCNT is a promising bioactivated composite coating for neural recording and stimulating.
Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, No. 27, Taiping Road, Beijing, 100850, China. qiuxialin0803@163.com wcy2000_te@yahoo.com.
Kun
K
Wang
Rong-Yu
RY
Tang
Xiao-Bo
XB
Zhao
Jun-Jie
JJ
Li
Yi-Ran
YR
Lang
Xiao-Xia
XX
Jiang
Hong-Ji
HJ
Sun
Qiu-Xia
QX
Lin
Chang-Yong
CY
Wang
D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
D019086:Bridged Bicyclo Compounds, Heterocyclic; D037742:Nanotubes, Carbon; D009842:Oligopeptides; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C047981:arginyl-glycyl-aspartic acid