Simple and efficient way to detect small polymorphic bands in plants.
There are many ways to detect polymorphism. In this study we use the microsatellite markers to detect the polymorphism for the salt tolerance. This method has been successfully conducted in Oryza sativa and Brassica juncea. The results are reproducible. In contrast to previous methods, our method is simple and quite accurate for detecting the polymorphic bands. In this study instead of using agarose gel and ethidium bromide staining, we used non-denaturing polyacrylamide gel and a low-cost improved method for silver staining when we compare it to 11 other methods for their ability to detect simple sequence repeat polymorphisms as small as 50 bp in denaturing polyacrylamide gels. All methods detected the same alleles and banding pattern. However, important differences in sensitivity, contrast, time consumption and background were observed.
Department of Life Science, Sogang University, Seoul 121-742, South Korea.
Manu
M
Kumar
Department of Life Science, Sogang University, Seoul 121-742, South Korea.
Seong Ryong
SR
Kim
Crop Improvement Division, Central Soil Salinity Research Institute, Karnal 132 001 (Haryana) India.
Prabodh Chander
PC
Sharma
Stress Physiology and Molecular Biology Laboratory, School of Life Science, Jawaharlal Nehru University, New Delhi 110067, India.
Ashwani
A
Pareek
Brassica juncea; Microsatellite; Oryza sativa; PAGE; SSR; SSR, simple sequence repeat; Silver staining; TBE, tris borate ethylenediaminetetracetic acid; TEMED, tetramethylethylenediamine