Physical preparation of alginate/chitosan polyelectrolyte complexes for biomedical applications.
Polyelectrolyte complexes represent a special class of polymeric compounds consisting of stoichiometric equivalents of oppositely charged polyions interacting together spontaneously to yield a complex in different forms. The present study aimed at preparing coacervates of alginate and chitosan polymers ready for casting as wound dressing films. This was based on controlling the pH of solutions and the reactions speed through controlling the rate of mixing of the polymers solutions together without using any water-miscible solvents. Alginate was modified with radiation and oxidation, and the interactions of the resulting chains and chitosan chains were tested with FTIR spectroscopy and scanning of the resulting films with SEM. This work showed the ability to prepare a complex of highly connected polymeric chains for further biomedical applications. This complex in the form of hydrogel could enhance the proliferation of cells in vitro and the healing efficiency with accelerating the wound closure rate as evidenced through the histological observations.
National Center for Radiation Research and Technology, Cairo, Egypt alamier@ymail.com.
Amir M
AM
Alsharabasy
Faculty of Science/Al-Azhar University, Cairo, Egypt.
Saad A
SA
Moghannem
Holding Company for Biological Products and Vaccines (VACSERA), Giza, Egypt.
Waleed N
WN
El-Mazny
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry; D005347:Fibroblasts / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D000071228:Polyelectrolytes / Q000737:chemistry*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D000071228:Polyelectrolytes; D003035:Cobalt; D020723:Glucuronic Acid; D048271:Chitosan
Alginate; chitosan; coacervation; polyelectrolyte complex; wound healing