Effect of extending the interval from Presynch to initiation of Ovsynch in a Presynch-Ovsynch protocol on fertility of timed artificial insemination services in lactating dairy cows.
The specific objective of this study was to determine if increasing the interval between the Presynch and Ovsynch portion of the Presynch-Ovsynch protocol (Presynch: PGF2α-14 d-PGF2α and Ovsynch: GnRH-7 d-PGF2α-56 h-GnRH-16-20 h-timed artificial insemination) from 12 to 14 d would reduce the fertility of lactating dairy cows not detected in estrus after Presynch that receive timed artificial insemination (TAI). Cows from 4 commercial dairy farms (n=3,165) were blocked by parity (primiparous vs. multiparous) and randomly assigned to a 12 (PSOv14-12; n=1,566) or 14 d (PSOv14-14; n=1,599) interval between the second PGF2α (PGF) injection of Presynch (P2) and the beginning of Ovsynch. Cows detected in estrus any time between P2 and the day of the TAI were inseminated (AIED group). From a subgroup of cows (177 and 150 in PSOv14-12 and PSOv14-14, respectively), ovarian parameters and ovulation were evaluated through determination of concentrations of progesterone (P4) in blood and transrectal ultrasonography at the time of the first GnRH (GnRH1) and the PGF injection of Ovsynch. Overall, 52.8% (n=1,671) of the cows were AIED, whereas 47.2% (n=1,494) received TAI. For cows that received TAI, pregnancies per artificial insemination 39 d after artificial insemination were similar for PSOv14-12 (36.3%) and PSOv14-14 (36.0%) but were greater for primiparous (41.5%) than multiparous cows (33.6%). Pregnancy loss from 39 to 105 d after artificial insemination was similar for PSOv14-12 (4.8%) and PSOv14-14 (8.6%), for primiparous (6.4%) and multiparous cows (7.0%), but a tendency for a treatment by parity interaction was observed. Both treatments had a similar proportion of cows with a follicle ≥ 10 mm and similar follicle size at GnRH1; however, the ovulatory response to GnRH was greater for PSOv14-12 (62.2%) than PSOv14-14 (46.4%). A greater proportion of cows with a functional corpus luteum (75.3 vs. 65.6%) and greater concentrations of P4 (3.9 vs. 3.3 ng/mL) at GnRH1 in PSOv14-14 than PSOv14-12 may have compensated for the reduction in fertility expected due to reduced ovulatory response to GnRH1. We concluded that extending the interval from Presynch to Ovsynch from 12 to 14 reduced ovulatory response to GnRH1 but did not reduce the fertility of cows that received TAI when cows were inseminated in estrus after presynchronization. Thus, farms that combine AIED and TAI during the Presynch-Ovsynch protocol may use a 14-d interval between Presynch and Ovsynch to simplify their management without reducing fertility of cows receiving TAI.
Department of Animal Science, Cornell University, Ithaca, NY 14853. Electronic address: jog25@cornell.edu.
J O
JO
Giordano
Dairy Health and Management Services LLC, Lowville, NY 13367.
M J
MJ
Thomas
Dairy Health and Management Services LLC, Lowville, NY 13367.
G
G
Catucuamba
Dairy Health and Management Services LLC, Lowville, NY 13367.
M D
MD
Curler
Department of Animal Science, Cornell University, Ithaca, NY 14853.
R
R
Wijma
Department of Animal Science, Cornell University, Ithaca, NY 14853.
M L
ML
Stangaferro
Department of Animal Science, Cornell University, Ithaca, NY 14853.
M
M
Masello
D000818:Animals; D002417:Cattle / Q000502:physiology; D003338:Corpus Luteum / Q000378:metabolism; D015237:Dinoprost / Q000008:administration & dosage / Q000097:blood*; D004971:Estrus / Q000378:metabolism; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000097:blood*; D007267:Injections; D007315:Insemination, Artificial / Q000662:veterinary*; D006080:Ovarian Follicle / Q000378:metabolism; D010060:Ovulation / Q000378:metabolism; D010298:Parity; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000097:blood
D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D015237:Dinoprost
Presynch-Ovsynch; dairy cow; estrus detection; timed artificial insemination