Chemical genetics and regeneration.
Regeneration involves interactions between multiple signaling pathways acting in a spatially and temporally complex manner. As signaling pathways are highly conserved, understanding how regeneration is controlled in animal models exhibiting robust regenerative capacities should aid efforts to stimulate repair in humans. One way to discover molecular regulators of regeneration is to alter gene/protein function and quantify effect(s) on the regenerative process: dedifferentiation/reprograming, stem/progenitor proliferation, migration/remodeling, progenitor cell differentiation and resolution. A powerful approach for applying this strategy to regenerative biology is chemical genetics, the use of small-molecule modulators of specific targets or signaling pathways. Here, we review advances that have been made using chemical genetics for hypothesis-focused and discovery-driven studies aimed at furthering understanding of how regeneration is controlled.
Wilmer Eye Institute, Johns Hopkins University, 400 N Broadway, 4015 Smith, Baltimore, MD 21205, USA.
Sumitra
S
Sengupta
Wilmer Eye Institute, Johns Hopkins University, 400 N Broadway, 4015 Smith, Baltimore, MD 21205, USA.
Liyun
L
Zhang
Wilmer Eye Institute, Johns Hopkins University, 400 N Broadway, 4015 Smith, Baltimore, MD 21205, USA.
Jeff S
JS
Mumm
D000818:Animals; D006801:Humans; D012038:Regeneration / Q000187:drug effects* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*
D054852:Small Molecule Libraries