[Isolation and identification of macrophages from liver of Microtus fortis].
OBJECTIVE
To separate and purify intrahepatic macrophages from Microtus fortis (Mf) and identify its phagocytosis.


METHODS
The intrahepatic macrophages from Mf were separated and purified by perfusion, collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity.


RESULTS
The macrophage cells from the liver of Mf were obtained. These cells were bright and circular, and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti-mouse CD14 antibody (Clone, Sa2-8) was about 50% of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink-phagocytosis experiment of macrophage cells from the liver of Mf was positive.


CONCLUSIONS
The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosomajaponicum.
Yuan
Y
Hu
Lei
L
Sun
Yu-xin
YX
Xu
Jian-ping
JP
Cao
D000818:Animals; D003411:Arvicolinae / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D008099:Liver / Q000166:cytology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis; D012554:Schistosomiasis japonica / Q000276:immunology*