An overview of the Progenika ID CORE XT: an automated genotyping platform based on a fluidic microarray system.
Automated testing platforms facilitate the introduction of red cell genotyping of patients and blood donors. Fluidic microarray systems, such as Luminex XMAP (Austin, TX), are used in many clinical applications, including HLA and HPA typing. The Progenika ID CORE XT (Progenika Biopharma-Grifols, Bizkaia, Spain) uses this platform to analyze 29 polymorphisms determining 37 antigens in 10 blood group systems. Once DNA has been extracted, processing time is approximately 4 hours. The system is highly automated and includes integrated analysis software that produces a file and a report with genotype and predicted phenotype results.
MD (corresponding author), Medical Services and Innovation, Canadian Blood Services, 1800 Alta Vista Drive, Ottawa, Ontario, Canada, K1G 4J5.
Mindy
M
Goldman
PhD, Immunohematology Laboratory, Banc de Sang i Teixits, Barcelona, Spain.
Núria
N
Núria
PhD, Molecular Immunohematology Laboratory, University of Campinas, Sao Paulo, Brazil.
Lilian M
LM
Castilho
D057205:Automation, Laboratory / Q000295:instrumentation / Q000379:methods*; D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
D001789:Blood Group Antigens; D004247:DNA