Transcriptome profiling of white adipose tissue in a mouse model for 15q duplication syndrome.
Obesity is not only associated with unhealthy lifestyles, but also linked to genetic predisposition. Previously, we generated an autism mouse model (patDp/+) that carries a 6.3 Mb paternal duplication homologous to the human 15q11-q13 locus. Chromosomal abnormalities in this region are known to cause autism spectrum disorder, Prader-Willi syndrome, and Angelman syndrome in humans. We found that, in addition to autistic-like behaviors, patDp/+ mice display late-onset obesity and hypersensitivity to a high-fat diet. These phenotypes are likely to be the results of genetic perturbations since the energy expenditures and food intakes of patDp/+ mice do not significantly differ from those of wild-type mice. Intriguingly, we found that an enlargement of adipose cells precedes the onset of obesity in patDp/+ mice. To understand the underlying molecular networks responsible for this pre-obese phenotype, we performed transcriptome profiling of white adipose tissue from patDp/+ and wild-type mice using microarray. We identified 230 genes as differentially expressed genes. Sfrp5 - a gene whose expression is positively correlated with adipocyte size, was found to be up-regulated, and Fndc5, a potent inducer of brown adipogenesis was identified to be the top down-regulated gene. Subsequent pathway analysis highlighted a set of 35 molecules involved in energy production, lipid metabolism, and small molecule biochemistry as the top candidate biological network responsible for the pre-obese phenotype of patDp/+. The microarray data were deposited in NCBI Gene Expression Omnibus database with accession number GSE58191. Ultimately, our dataset provides novel insights into the molecular mechanism of obesity and demonstrated that patDp/+ is a valuable mouse model for obesity research.
RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan.
Xiaoxi
X
Liu
RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan ; Graduate School of Biomedical Sciences, Hiroshima University, Minami, Hiroshima 734-8553, Japan.
Kota
K
Tamada
RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan ; Graduate School of Biomedical Sciences, Hiroshima University, Minami, Hiroshima 734-8553, Japan.
Rui
R
Kishimoto
RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan.
Hiroko
H
Okubo
Banyu Tsukuba Research Institute, Tsukuba, Ibaraki 300-2611, Japan.
Satoko
S
Ise
Banyu Tsukuba Research Institute, Tsukuba, Ibaraki 300-2611, Japan.
Hisashi
H
Ohta
Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Sandra
S
Ruf
Molecular Neuroscience Research Center, Shiga University of Medical Science, Ohtsu, Shiga 520-2192, Japan.
Jin
J
Nakatani
Graduate School of Biomedical Sciences, Hiroshima University, Minami, Hiroshima 734-8553, Japan.
Nobuoki
N
Kohno
Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
François
F
Spitz
RIKEN Brain Science Institute, Wako, Saitama 351-0198, Japan ; Graduate School of Biomedical Sciences, Hiroshima University, Minami, Hiroshima 734-8553, Japan ; JST, CREST, Japan.
Toru
T
Takumi
15q duplication syndrome; Fndc5; Microarray; Obesity; Sfrp5