Determination of typical lipophilic marine toxins in marine sediments from three coastal bays of China using liquid chromatography-tandem mass spectrometry after accelerated solvent extraction.
A method based on sample preparation by accelerated solvent extraction and analysis by liquid chromatography-tandem mass spectrometry was validated and used for determination of seven typical lipophilic marine toxins (LMTs) in marine sediment samples collected from three typical coastal bays in China. Satisfactory specificity, reproducibility (RSDs ≤ 14.76%), stability (RSDs ≤ 17.37%), recovery (78.0%-109.0%), and detection limit (3.440 pg/g-61.85 pg/g) of the developed method were achieved. The results obtained from the analysis of samples from Hangzhou Bay revealed okadaic acid as the predominant LMT with concentrations ranging from 186.0 to 280.7 pg/g. Pecenotoxin-2 was quantified in sediment samples from Laizhou Bay at the concentrations from 256.4 to 944.9 pg/g. These results suggested that the proposed method was reliable for determining the typical LMTs in marine sediments and that the sediments obtained from Hangzhou Bay, Laizhou Bay and Jiaozhou Bay were all contaminated by certain amounts of LMTs.
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Yanlong
Y
Wang
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China. Electronic address: craige2000@hotmail.com.
Junhui
J
Chen
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Zhaoyong
Z
Li
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Shuai
S
Wang
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Qian
Q
Shi
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Wei
W
Cao
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Xiaoling
X
Zheng
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Chengjun
C
Sun
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Xiaoru
X
Wang
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.
Li
L
Zheng
D061006:Bays / Q000737:chemistry; D002681:China; D002853:Chromatography, Liquid / Q000379:methods*; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D019015:Geologic Sediments / Q000032:analysis*; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D008387:Marine Toxins; D012997:Solvents; D014874:Water Pollutants, Chemical
LC–MS/MS; Lipophilic marine toxins; Okadaic acid; Pecenotoxin-2; Pressurized liquid extraction; Sediment