Differential Impact of Plasma Proteins on the Adhesion Efficiency of Vascular-Targeted Carriers (VTCs) in Blood of Common Laboratory Animals.
Vascular-targeted carrier (VTC) interaction with human plasma is known to reduce targeted adhesion efficiency in vitro. However, the role of plasma proteins on the adhesion efficiency of VTCs in laboratory animals remains unknown. Here, in vitro blood flow assays are used to explore the effects of plasma from mouse, rabbit, and porcine on VTC adhesion. Porcine blood exhibited a strong negative plasma effect on VTC adhesion while no significant plasma effect was found with rabbit and mouse blood. A brush density poly(ethylene glycol) (PEG) on VTCs was effective at improving adhesion of microsized, but not nanosized, VTCs in porcine blood. Overall, the results suggest that porcine models, as opposed to mouse, can serve as better models in preclinical research for predicting the in vivo functionality of VTCs for use in humans. These considerations hold great importance for the design of various pharmaceutical products and development of reliable drug delivery systems.
Department of Chemical Engineering, University of Michigan , 2800 Plymouth Road, Ann Arbor, Michigan 48109, United States.
Katawut
K
Namdee
Department of Chemical Engineering, University of Michigan , 2800 Plymouth Road, Ann Arbor, Michigan 48109, United States.
Daniel J
DJ
Sobczynski
Department of Chemical Engineering, University of Michigan , 2800 Plymouth Road, Ann Arbor, Michigan 48109, United States.
Peter J
PJ
Onyskiw
Department of Chemical Engineering, University of Michigan , 2800 Plymouth Road, Ann Arbor, Michigan 48109, United States.
Omolola
O
Eniola-Adefeso
D000818:Animals; D001783:Blood Flow Velocity; D001798:Blood Proteins / Q000378:metabolism*; D002448:Cell Adhesion; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D013552:Swine
D001798:Blood Proteins; D004337:Drug Carriers; D011092:Polyethylene Glycols