Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine.
Stem cells switch between asymmetric and symmetric division to expand in number as tissues grow during development and in response to environmental changes. The stem cell intrinsic proteins controlling this switch are largely unknown, but one candidate is the Lin-28 pluripotency factor. A conserved RNA-binding protein that is downregulated in most animals as they develop from embryos to adults, Lin-28 persists in populations of adult stem cells. Its function in these cells has not been previously characterized. Here, we report that Lin-28 is highly enriched in adult intestinal stem cells in the Drosophila intestine. lin-28 null mutants are homozygous viable but display defects in this population of cells, which fail to undergo a characteristic food-triggered expansion in number and have reduced rates of symmetric division as well as reduced insulin signaling. Immunoprecipitation of Lin-28-bound mRNAs identified Insulin-like Receptor (InR), forced expression of which completely rescues lin-28-associated defects in intestinal stem cell number and division pattern. Furthermore, this stem cell activity of lin-28 is independent of one well-known lin-28 target, the microRNA let-7, which has limited expression in the intestinal epithelium. These results identify Lin-28 as a stem cell intrinsic factor that boosts insulin signaling in intestinal progenitor cells and promotes their symmetric division in response to nutrients, defining a mechanism through which Lin-28 controls the adult stem cell division patterns that underlie tissue homeostasis and regeneration.
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Ching-Huan
CH
Chen
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Arthur
A
Luhur
Department of Biology, Indiana University, Bloomington, IN 47405, USA nsokol@indiana.edu.
Nicholas
N
Sokol
D000818:Animals; D002455:Cell Division; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development*; D005260:Female; D005838:Genotype; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006720:Homozygote; D007328:Insulin / Q000378:metabolism; D007413:Intestinal Mucosa / Q000166:cytology* / Q000254:growth & development*; D035683:MicroRNAs / Q000378:metabolism; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology; D012038:Regeneration; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology*; D013696:Temperature
D029721:Drosophila Proteins; D007328:Insulin; C531976:Let-7 microRNA, Drosophila; C000605000:Lin-28 protein, Drosophila; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D049452:Green Fluorescent Proteins; C094982:InR protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases
Drosophila; Intestinal stem cell; Lin-28; Symmetric renewal