A programmable Y-shaped junction scaffold-mediated modular and cascade amplification strategy for the one-step, isothermal and ultrasensitive detection of target DNA.
The programmable DNA polymerization across the two branches of the assembled Y-shaped junction was ingeniously manipulated for modular target recycling and cascade lambda exonuclease cleavage, which afforded the one-pot, isothermal and ultrasensitive detection of target DNA. A low detection limit of 28.2 fM of target DNA with an excellent selectivity could be obtained.
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, 53 Zhengzhou Road, Qingdao 266042, China. sliu@qust.edu.cn.
Shufeng
S
Liu
Hongwei
H
Gong
Xinya
X
Sun
Tao
T
Liu
Li
L
Wang
D010582:Bacteriophage lambda; D020029:Base Pairing; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D005453:Fluorescence; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques; D013696:Temperature; D014764:Viral Proteins / Q000737:chemistry*
D015342:DNA Probes; D014764:Viral Proteins; D004247:DNA; D005090:Exodeoxyribonucleases; C043586:exo protein, Bacteriophage lambda