Structurally Characterized Zn2+ Selective Ratiometric  Fluorescence Probe in 100 % Water for HeLa Cell Imaging:  Experimental and Computational Studies.
Fluorescence recognition of Zn2+ in 100% aqueous medium using 2-((1, 3 dihydroxy-2-(hydroxymethyl)propan-2 ylimino) methyl) phenol (SALTM) as ratiometric probe is reported. Moreover, SALTM can discriminate Zn2+ from Cd2+very effectively. The binding constant and detection limit of the probe for Zn2+ is 2.2×10(4) M(-1/2) and 2.79×10(-8) M respectively.Interestingly, corresponding naphthalene derivative(HNTM) having less water solubility fails to be a ratiometric sensor. SALTM can detect intracellular Zn2+ in HeLa cervical cancer cells under fluorescence microscope. Moreover, DFT and TD-DFT studies support experimental findings.
Babli
B
Kumari
Sisir
S
Lohar
Milan
M
Ghosh
Sabyasachi
S
Ta
Archya
A
Sengupta
Prajna Paramita
PP
Banerjee
Ansuman
A
Chattopadhyay
Debasis
D
Das
D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D011407:Propane / Q000031:analogs & derivatives* / Q000737:chemistry; D011789:Quantum Theory; D014867:Water / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*
C000611340:2-((1, 3 dihydroxy-2-(hydroxymethyl)propan-2 ylimino)methyl) phenol; D005456:Fluorescent Dyes; D010636:Phenols; D014867:Water; D015032:Zinc; D011407:Propane