Three dimensional imaging of gold-nanoparticles tagged samples using phase retrieval with two focus planes.
Optical sectioning microscopy can provide highly detailed three dimensional (3D) images of biological samples. However, it requires acquisition of many images per volume, and is therefore time consuming, and may not be suitable for live cell 3D imaging. We propose the use of the modified Gerchberg-Saxton phase retrieval algorithm to enable full 3D imaging of gold-particle tagged samples using only two images. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. The proposed concept is presented and validated both on simulated data as well as experimentally.
Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel.|The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
Tali
T
Ilovitsh
Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel.|The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
Asaf
A
Ilovitsh
Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel.|The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
Aryeh
A
Weiss
Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel.
Rinat
R
Meir
Faculty of Engineering, Bar Ilan University, Ramat-Gan 5290002, Israel.|The Bar-Ilan Institute of Nanotechnology &Advanced Materials, Bar Ilan University, Ramat-Gan 5290002, Israel.
Zeev
Z
Zalevsky
D045744:Cell Line, Tumor; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*
D006046:Gold