Preparation of Stabilizer-Free Silver Nanoparticle-Coated Micropipettes as Surface-Enhanced Raman Scattering Substrate for Single Cell Detection.
In this work, we established a convenient while reproduceable method for stabilizer-free silver nanoparticle (AgNP)-coated micropipettes by the combination of magnetron sputtering and surface coupling agent. The clear surfaces of the AgNPs are beneficial for absorbing biological or functional molecules on their surfaces. By optimizing the operating parameters, such as sputtering current and sputtering time, the tip of micropipettes coated with AgNPs exhibits excellent surface-enhanced Raman scattering (SERS) performance. Finally, the Raman spectra of a single A549 lung adenocarcinoma cell are successfully acquired by these advanced SERS-active micropipettes.
Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, 2 Sipailou, Nanjing, 210096, People's Republic of China.
Yi-Bin
YB
Tan
Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, 2 Sipailou, Nanjing, 210096, People's Republic of China.
Jie-Meng
JM
Zou
Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Sciences and Medical Engineering, Southeast University, 2 Sipailou, Nanjing, 210096, People's Republic of China. guning@seu.edu.cn.|Suzhou Key Lab of Biomedical Materials and Technology, Research Institute of Southeast University in Suzhou, 150 Ren Ai Road, Suzhou Industrial Park, Suzhou, 215123, People's Republic of China. guning@seu.edu.cn.
Ning
N
Gu
Magnetron sputtering; Micropipette; SERS; Silver nanoparticles; Single cell detection