Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase.
Synthetic chain terminators were used to capture the biosynthetic intermediates from a partially reducing iterative type I polyketide synthase, which is integrated into a multimodular biosynthesis enzyme. The off-loaded metabolites clarified the timing of ketoreduction and aromatization in the assembly of the antibiotic micacocidin.
Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Adolf-Reichwein-Str. 23, D-07745 Jena, Germany. markus.nett@hki-jena.de.
Hirokazu
H
Kage
Elena
E
Riva
James S
JS
Parascandolo
Martin F
MF
Kreutzer
Manuela
M
Tosin
Markus
M
Nett
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D053898:Biosynthetic Pathways; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D043368:Ralstonia solanacearum / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009942:Organometallic Compounds; C112186:micacocidin A; D048630:Polyketide Synthases