Antibiotic resistance monitoring in Vibrio spp. isolated from rearing environment and intestines of abalone Haliotis diversicolor.
546 Vibrio isolates from rearing seawater (292 strains) and intestines of abalone (254 strains) were tested to ten antibiotics using Kirby-Bauer diffusion method. Resistant rates of abalone-derived Vibrio isolates to chloramphenicol (C), enrofloxacin (ENX) and norfloxacin (NOR) were <28%, whereas those from seawater showed large fluctuations in resistance to each of the tested antibiotics. Many strains showed higher resistant rates (>40%) to kanamycin (KNA), furazolidone (F), tetracycline (TE), gentamicin (GM) and rifampin (RA). 332 isolates from seawater (n=258) and abalone (n=74) were resistant to more than three antibiotics. Peaked resistant rates of seawater-derived isolates to multiple antibiotics were overlapped in May and August. Statistical analysis showed that pH had an important effect on resistant rates of abalone-derived Vibrio isolates to RA, NOR, and ENX. Salinity and dissolved oxygen were negatively correlated with resistant rates of seawater-derived Vibrio isolates to KNA, RA, and PG.
College of Life Science, South China Normal University, Guangzhou 510631, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China; Key Laboratory of Fishery Ecology and Environment, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangdong Province, Guangzhou 510300, China.
R X
RX
Wang
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China; Key Laboratory of Fishery Ecology and Environment, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangdong Province, Guangzhou 510300, China.
J Y
JY
Wang
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China.
Y C
YC
Sun
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangdong Province, Guangzhou 510300, China.
B L Yang
College of Life Science, South China Normal University, Guangzhou 510631, China; Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China. Electronic address: wangruixuan@scsfri.ac.cn.
A L Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D004352:Drug Resistance, Microbial; D049851:Gastropoda / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D054712:Salinity; D012623:Seawater / Q000382:microbiology; D014733:Vibrio / Q000187:drug effects* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents
Abalone; Antibiotic resistance; Aquaculture; Vibrio