Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.
An advanced methicillin-resistant Staphylococcus aureus (MRSA) detection PCR approach targeting SCCmec-orfX along with mecA and mecC was evaluated for S. aureus and coagulase-negative staphylococci. The possession of mecA and/or mecC was correctly confirmed in all cases. All methicillin-susceptible S. aureus strains (n = 98; including staphylococcal cassette chromosome mec element [SCCmec] remnants) and 98.1% of the MRSA strains (n = 160, including 10 mecC-positive MRSA) were accurately categorized.
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany kbecker@uni-muenster.de.
Karsten
K
Becker
Staphylococcus National Reference Centre, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
Olivier
O
Denis
Staphylococcus National Reference Centre, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium.
Sandrine
S
Roisin
Institute of Hygiene, University Hospital Münster, Münster, Germany.
Alexander
http://orcid.org/0000-0002-0649-5185
A
Mellmann
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Evgeny A
EA
Idelevich
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Dennis
D
Knaack
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Sarah
S
van Alen
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
André
A
Kriegeskorte
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Robin
R
Köck
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Frieder
F
Schaumburg
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Georg
G
Peters
Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Britta
B
Ballhausen
D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology