Data for chromosome contacts and matched transcription profiles at three cell cycle phases in the fission yeast.
The data described in this article pertains to Grand et al. (2014), "Chromosome conformation maps in fission yeast reveal cell cycle dependent sub nuclear structure" [1]. Temperature sensitive Schizosaccharomyces pombe cell division cycle (cdc) mutants, which are induced by a shift in temperature to 36 °C, were chosen for the analysis of genome structure in the G1 phase, G2 phase and mitotic anaphase of the cell cycle. Chromatin and total RNA were isolated from the same cell culture following synchronization. Two biological replicates were analyzed for each condition. The global, three-dimensional organization of the chromosomes was captured at high resolution using Genome Conformation Capture (GCC). GCC libraries and RNA samples were sequenced using an Illumina Hi-Seq 2000 platform (Beijing Genomics Institute (China)). DNA sequences were processed using the Topography suite v1.19 [2] to obtain chromosome contact frequency matrices. RNA sequences were processed using the Cufflinks pipeline [3] to measure gene transcript levels and how these varied between the conditions. All sequence data, processed GCC and transcriptome files are available under the Gene Expression Omnibus (GEO) accession number GSE52287 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52287).
Liggins institute, University of Auckland, Grafton Auckland 1032, New Zealand ; Institute of Natural and Mathematical Sciences, Massey University, Albany, Auckland 0745, New Zealand.
Ralph S
RS
Grand
Liggins institute, University of Auckland, Grafton Auckland 1032, New Zealand.
Justin M
JM
O'Sullivan