Serine-arginine protein kinase 1 (SRPK1), a determinant of angiogenesis, is upregulated in prostate cancer and correlates with disease stage and invasion.
Vascular endothelial growth factor (VEGF) undergoes alternative splicing to produce both proangiogenic and antiangiogenic isoforms. Preferential splicing of proangiogenic VEGF is determined by serine-arginine protein kinase 1 (SRPK1), which is upregulated in a number of cancers. In the present study, we aimed to investigate SRPK1 expression in prostate cancer (PCa) and its association with cancer progression. SRPK1 expression was assessed using immunohistochemistry of PCa tissue extracted from radical prostatectomy specimens of 110 patients. SRPK1 expression was significantly higher in tumour compared with benign tissue (p<0.00001) and correlated with higher pT stage (p=0.004), extracapsular extension (p=0.003) and extracapsular perineural invasion (p=0.008). Interestingly, the expression did not correlate with Gleason grade (p=0.21), suggesting that SRPK1 facilitates the development of a tumour microenvironment that favours growth and invasion (possibly through stimulating angiogenesis) while having little bearing on the morphology or function of the tumour cells themselves.
School of Physiology and Pharmacology, University of Bristol, Bristol, UK.
Nicholas
N
Bullock
Department of Urology, Southmead Hospital, Bristol, UK.
Jonathan
J
Potts
School of Social and Community Medicine, University of Bristol, Bristol, UK.
Andrew J
AJ
Simpkin
Department of Urology, Southmead Hospital, Bristol, UK.
Anthony
A
Koupparis
School of Physiology and Pharmacology, University of Bristol, Bristol, UK.
Steve J
SJ
Harper
Department of Cellular Pathology, Southmead Hospital, Bristol, UK.
Jon
J
Oxley
School of Physiology and Pharmacology, University of Bristol, Bristol, UK.
Sebastian
S
Oltean
D000328:Adult; D000368:Aged; D042501:Angiogenic Proteins / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000601:surgery; D017346:Protein Serine-Threonine Kinases / Q000032:analysis*; D018570:Risk Assessment; D012307:Risk Factors; D016896:Treatment Outcome; D059016:Tumor Microenvironment; D015854:Up-Regulation
D042501:Angiogenic Proteins; D014408:Biomarkers, Tumor; C087728:SRPK1 protein, human; D017346:Protein Serine-Threonine Kinases
ANGIOGENESIS; CANCER; PROSTATE