Accelerated bone ingrowth by local delivery of Zinc from bioactive glass: oxidative stress status, mechanical property, and microarchitectural characterization in an ovariectomized rat model.
Synthetic bone graft substitutes such as bioactive glass (BG) material are developed in order to achieve successful bone regeneration. Zn plays an important role in the proper bone growth, development, and maintenance of healthy bones.



This study aims to evaluate in vivo the performance therapy of zinc-doped bioactive glass (BG-Zn) and its applications in biomedicine.



Female Wistar rats were ovariectomized. BG and BG-Zn were implanted in the femoral condyles of Wistar rats and compared to that of control group. Grafted bone tissues were carefully removed to evaluate the oxidative stress status, histomorphometric profile, mechanical property, and mineral bone distribution by using inductively coupled plasma optical emission spectrometry.



A significant decrease of thiobarbituric acid-reactive substances was observed after BG-Zn implantation. Superoxide dismutase, catalase (CAT), and glutathione peroxidase (GPx) activities significantly increased in ovariectomized group implanted with Zinc-doped bioactive glass (OVX-BG-Zn) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). An improved mechanical property was noticed in contact of OVX-BG-Zn (39±6 HV) when compared with that of OVX-BG group (26±9 HV). After 90 days of implantation, the histomorphometric analysis showed that trabecular thickness (Tb.Th) and trabecular number (Tb.N) were significantly increased with 28 and 24%, respectively, in treated rats of OVX-BG-Zn group as compared to those of OVX-BG groups. Trabecular separation (Tb.Sp) and trabecular bone pattern factor (TBPf) were significantly decreased in OVX-BG-Zn group with 29.5 and 54% when compared with those of OVX-BG rat groups. On the other hand, a rise in Ca and P ion concentrations in the implanted microenvironment was shown and lead to the formation/deposition of Ca-P phases. The ratio of pyridinoline [Pyr] to dihydroxylysinonorleucine [DHLNL] cross-links was normalized to the control level.



Our findings suggested that BG-Zn might have promising potential applications for osteoporosis therapy.
Campus de Beaulieu, UMR CNRS 6226, University of Rennes, Rennes, France.|Animal Ecophysiology Laboratory, Department of Life Sciences, Sfax Faculty of Science, University of Sfax, Sfax, Tunisia.|Higher Institute of Applied Biology of Medenine, University of Gabes, Gabes, Tunisia; jbahisamira@yahoo.fr.
Jbahi
J
Samira
Animal Ecophysiology Laboratory, Department of Life Sciences, Sfax Faculty of Science, University of Sfax, Sfax, Tunisia.
Monji
M
Saoudi
Histology, Orthopaedic and Traumatology Laboratory, Sfax Faculty of Medicine, University of Sfax, Sfax, Tunisia.
Kabir
K
Abdelmajid
Campus de Beaulieu, UMR CNRS 6226, University of Rennes, Rennes, France.
Oudadesse
O
Hassane
Histology, Orthopaedic and Traumatology Laboratory, Sfax Faculty of Medicine, University of Sfax, Sfax, Tunisia.
Rebai
R
Treq
Animal Ecophysiology Laboratory, Department of Life Sciences, Sfax Faculty of Science, University of Sfax, Sfax, Tunisia.
Efeki
E
Hafed
Campus de Beaulieu, UMR CNRS 6226, University of Rennes, Rennes, France.
Elfeki
E
Abdelfatteh
Histology, Orthopaedic and Traumatology Laboratory, Sfax Faculty of Medicine, University of Sfax, Sfax, Tunisia.
Keskes
K
Hassib
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000295:instrumentation / Q000379:methods; D002516:Ceramics; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005269:Femur / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055595:Mechanical Phenomena; D023421:Models, Animal; D010024:Osteoporosis / Q000628:therapy*; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D015032:Zinc / Q000008:administration & dosage*
C022107:Bioglass; D018786:Bone Substitutes; D015032:Zinc
bioactive glass; mechanical property; osteoporosis; oxidative stress; trabecular bone architecture; zinc metallic ion