Transdentinal cytotoxicity of resin-based luting cements to pulp cells.
OBJECTIVE
The aim of this study was to evaluate the transdentinal cytotoxicity of components released from different resin-based luting cements to cultured MDPC-23 odontoblast-like cells and human dental pulp cells (HDPCs).


METHODS
Artificial pulp chamber (APC)/dentin disc sets were distributed into four groups according to the materials tested (n = 10), as follows: G1, control (no treatment); G2, resin-modified glass-ionomer cement (RelyX Luting 2); G3, self-adhesive resin cement (RelyX U200); and G4, conventional resin cement (RelyX ARC). The materials were applied to the occlusal surfaces (facing up) of the dentin discs adapted to the APCs. The pulpal surfaces of the discs were maintained in contact with culture medium. Then, an aliquot of 400 μL from the extract (culture medium + resin-based components that diffused through dentin) of each luting cement was applied for 24 h to HDPCs or MDPC-23 cells previously seeded in wells of 24-well plates. Cell viability analysis was performed by the MTT assay (1-way ANOVA/Tukey test; α = 5 %).


RESULTS
For MDPC-23 cells, RelyX ARC (G4) and RelyX Luting 2 (G2) caused greater reduction in cell viability compared with the negative control group (P < 0.05). Only the HDPCs exposed to RelyX ARC (G4) extract showed a tendency toward viability decrease (9.3 %); however, the values were statistically similar to those of the control group (G1) (P > 0.05).


CONCLUSIONS
In accordance with the safe limits of ISO 10993-5:1999 (E) recommendations, all resin-based luting cements evaluated in this study can be considered as non-toxic to pulp cells.


CONCLUSIONS
Cytotoxicity of resin-based luting cements is material-dependent, and the different protocols for the application of these dental materials to dentin may interfere with their cytotoxicity.
Department of Physiology and Pathology, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Humaitá Street, 1680, Araraquara, SP, Postal code: 14801-903, Brazil.
Lucas
L
da Fonseca Roberti Garcia
Department of Physiology and Pathology, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Humaitá Street, 1680, Araraquara, SP, Postal code: 14801-903, Brazil.
Elaine Cristina Voltolini
EC
Pontes
Department of Physiology and Pathology, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Humaitá Street, 1680, Araraquara, SP, Postal code: 14801-903, Brazil.
Fernanda Gonçalves
FG
Basso
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Araraquara, SP, Brazil.
Josimeri
J
Hebling
Department of Physiology and Pathology, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Humaitá Street, 1680, Araraquara, SP, Postal code: 14801-903, Brazil.
Carlos Alberto
CA
de Souza Costa
Department of Physiology and Pathology, Araraquara School of Dentistry, Universidade Estadual Paulista-UNESP, Humaitá Street, 1680, Araraquara, SP, Postal code: 14801-903, Brazil. diana_odonto@yahoo.com.br.
Diana Gabriela
DG
Soares
D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity*; D002470:Cell Survival; D003188:Composite Resins / Q000633:toxicity*; D003738:Dental Cements / Q000633:toxicity*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000633:toxicity*; D019279:Resin Cements / Q000633:toxicity*
D003188:Composite Resins; D003738:Dental Cements; D005899:Glass Ionomer Cements; D011109:Polymethacrylic Acids; C462240:RelyX ARC; C587242:RelyX U200; D019279:Resin Cements; C084550:Vitremer; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate
Cytotoxicity; Dentin; Luting cements; Pulp cell