Gene expression profiling of mononuclear cells from patients with sepsis secondary to community-acquired pneumonia.
Mechanisms governing the inflammatory response during sepsis involve crosstalk between diverse signaling pathways, but current knowledge provides an incomplete picture of the syndrome. Microarray-based expression profiling is a powerful approach for the investigation of complex clinical conditions such as sepsis. In this study, we investigated whole-genome expression profiles in mononuclear cells from septic patients admitted in intensive care units with community-acquired pneumonia. Blood samples were collected at the time of sepsis diagnosis and seven days later since we aimed to evaluate the role of biological processes or genes possibly involved in patient recovery. Here we provide a detailed description of the study design, including clinical information, experimental methods and procedures regarding data analysis. Metadata corresponding to microarray results deposited in the database Gene Expression Omnibus (GEO) under the accession number GSE48080 are also described in this report. Our dataset allows the identification of genes possibly associated with host defense to infection as well as gene expression patterns associated with patient outcome.
Center for Experimental Research, Instituto Israelita de Ensino e Pesquisa, Hospital Israelita Albert Einstein, Sao Paulo, Brazil.
Patricia
P
Severino
Intensive Care Unit, Hospital Israelita Albert Einstein, Sao Paulo, Brazil.
Eliezer
E
Silva
Division of Infectious Diseases, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Giovana Lotici
GL
Baggio-Zappia
Division of Infectious Diseases, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Milena Karina Colo
MK
Brunialti
Division of Infectious Diseases, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Laura Alejandra
LA
Nucci
Intensive Care Unit, Hospital Sirio Libanes, Sao Paulo, Brazil.
Otelo Rigato
OR
Junior
Department of Gynecology, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Ismael Dale Cotrim Guerreiro
ID
da Silva
Intensive Care Unit, Hospital Sao Paulo, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Flavia Ribeiro
FR
Machado
Division of Infectious Diseases, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
Reinaldo
R
Salomao
DNA microarray; Gene expression profiling; Patient outcome; Sepsis