[Infrared fluorescent markers for microarray DNA analysis on biological microchip].
To expand the informational capabilities of molecular genetic research, on the biological microchips, new indotricarbocyanine dyes that fluoresce in the near infrared (IR) spectral region have been synthesized. The developed IR dyes were studied using a biochip-based test system for detection of mutations in the BRCA1/BRCA2 and CHECK2 genes associated with breast cancer. The fluorescent label was introduced to the analyzed DNA during PCR using primers labeled with the synthesized IR dyes. An analyzer that allows recording and processing of images of fluorescent microarrays in the IR spectral region was designed and manufactured. It has been shown that the use of the synthesized dyes enables to conduct analysis in the IR region and improve the reliability of medical diagnostic tests due to low fluorescence intensity of sample components as well as of a biochip substrate and the reagents used for analysis.
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
M A
MA
Spitsyn
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
V E
VE
Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
V E
VE
Kuznetsova
Biochip-EIMB, Moscow, 117312, Russia.
V E
VE
Barsky
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
E E
EE
Egorov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
M A
MA
Emelyanova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
E Ya
EY
Kreindlin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
Yu P
YP
Lysov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
T O
TO
Guseinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
D E
DE
Fesenko
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
S A
SA
Lapa
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
S A
SA
Surzhikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
I S
IS
Abramov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
T V
TV
Nasedkina
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
A S
AS
Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.|chud@eimb.ru.
A V
AV
Chudinov
D019313:BRCA1 Protein / Q000235:genetics / Q000378:metabolism; D024682:BRCA2 Protein / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D002232:Carbocyanines / Q000138:chemical synthesis* / Q000378:metabolism; D064447:Checkpoint Kinase 2 / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000138:chemical synthesis / Q000235:genetics; D005260:Female; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007259:Infrared Rays; D056656:Lab-On-A-Chip Devices; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods
D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D002232:Carbocyanines; D017931:DNA Primers; D005456:Fluorescent Dyes; D064447:Checkpoint Kinase 2
IR fluorescent dyes; Microarrays; microarray analyzer; molecular genetic analysis; mutations