Mycoplasma bovis-derived lipid-associated membrane proteins activate IL-1β production through the NF-κB pathway via toll-like receptor 2 and MyD88.
Mycoplasma bovis causes pneumonia, otitis media, and arthritis in young calves, resulting in economic losses to the cattle industry worldwide. M. bovis pathogenesis results in part from excessive immune responses. Lipid-associated membrane proteins (LAMPs) can potently induce host innate immunity. However, interactions between M. bovis-derived LAMPs and Toll-like receptors (TLRs), or signaling pathways eliciting active inflammation and NF-κB activation, are incompletely understood. Here, we found that IL-1β expression was induced in embryonic bovine lung (EBL) cells stimulated with M. bovis-derived LAMPs. Subcellular-localization analysis revealed nuclear p65 translocation following EBL cell stimulation with M. bovis-derived LAMPs. An NF-κB inhibitor reversed M. bovis-derived LAMP-induced IL-1β expression. TLR2 and myeloid differentiation primary response gene 88 (MyD88) overexpression increased LAMP-dependent IL-1β induction. TLR2-neutralizing antibodies reduced IL-1β expression during LAMP stimulation. LAMPs also inhibited IL-1β expression following overexpression of a dominant-negative MyD88 protein. These results suggested that M. bovis-derived LAMPs activate IL-1β production through the NF-κB pathway via TLR2 and MyD88.
National Contagious Bovine Pleuropneumonia Reference Laboratory, Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, 150001, China; Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China.
Yang
Y
Wang
National Contagious Bovine Pleuropneumonia Reference Laboratory, Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, 150001, China.
Suli
S
Liu
National Contagious Bovine Pleuropneumonia Reference Laboratory, Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, 150001, China.
Yuan
Y
Li
College of Resources and Environmental, Northeast Agricultural University, Harbin, 150001, China.
Qi
Q
Wang
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130000, China.
Jiari
J
Shao
College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150001, China.
Ying
Y
Chen
National Contagious Bovine Pleuropneumonia Reference Laboratory, Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, 150001, China. Electronic address: xinjiuqing2001@126.com.
Jiuqing
J
Xin
D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism*; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D007113:Immunity, Innate; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008168:Lung / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D051907:Lysosomal Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D009175:Mycoplasma Infections / Q000276:immunology*; D045682:Mycoplasma bovis / Q000276:immunology*; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D009570:Nitriles / Q000494:pharmacology; D015398:Signal Transduction; D013450:Sulfones / Q000494:pharmacology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology / Q000378:metabolism
C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D019138:Antibodies, Blocking; D000942:Antigens, Bacterial; D053583:Interleukin-1beta; D051907:Lysosomal Membrane Proteins; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D051195:Toll-Like Receptor 2
IL-1β; Mycoplasma bovis-derived LAMPs; NF-κB