Characteristics of stem cells from human exfoliated deciduous teeth (SHED) from intact cryopreserved deciduous teeth.
The aim of this study is to compare the characteristics of stem cells derived from human exfoliated deciduous teeth (SHED) from cryopreserved intact deciduous teeth with those of fresh SHED. In total, 20 exfoliated deciduous teeth were randomly divided into a fresh group (f-SHED; n = 11) and cryopreserved group (c-SHED; n = 9; stored for 1-8 months). Following thawing and separation of the pulp, the SHED cells were cultured, and the characteristics as mesenchymal stem cells were investigated using proliferation assays, cell-cycle analysis, colony-forming unit-fibroblast (CFU-F) assays, and flow cytometry analyses. Furthermore, differentiation into adipogenic and osteogenic lineages was investigated in vitro as well as in vivo via transplantation in mice. We found no significant differences between the two groups in the proliferation analyses, in the expression of mesenchymal stem cell markers, or in the adipogenic and osteogenic differentiation in vitro (p < 0.05). Furthermore, the in vivo transplantation results showed no significant differences in the quantity of bone tissue that formed or in histochemistry performance (p < 0.05). In conclusion, cryopreservation of intact exfoliated deciduous teeth appears to be a useful method for preserving SHED.
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul, South Korea.
Hyo-Seol
HS
Lee
Mijeong
M
Jeon
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
Mi Jung
MJ
Jeon
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
Seong-Oh
SO
Kim
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
Seung-Hye
SH
Kim
Jae-Ho
JH
Lee
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
Jea-Ho
JH
Lee
Department of Biomaterials & Prosthodontics, Kyung Hee University Hospital at Gangdong, School of Dentistry, Kyung Hee University, Seoul, South Korea.
Su-Jin
SJ
Ahn
Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea.
Yooseok
Y
Shin
Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, South Korea. Electronic address: songjs@yuhs.ac.
Je Seon
JS
Song
D000818:Animals; D002454:Cell Differentiation; D015925:Cryopreservation / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D013234:Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*
Cryopreservation; Deciduous teeth; Dental pulp stem cells; Frozen teeth; Intact teeth; In vivo; Mesenchymal stem cells; SHED; Stem cells; Stem cells from human exfoliated deciduous teeth