Low-Cost Synthesis of Smart Biocompatible Graphene Oxide Reduced Species by Means of GFP.
The aim of this work is focused on the engineering of biocompatible complex systems composed of an inorganic and bio part. Graphene oxide (GO) and/or graphite oxide (GtO) were taken into account as potential substrates to the linkage of the protein such as Anemonia sulcata recombinant green fluorescent protein (rAsGFP). The complex system is obtained through a reduction process between GO/GtO and rAsGFP archiving an environmentally friendly biosynthesis. Spectroscopic measurements support the formation of reduced species. In particular, photoluminescence shows a change in the activity of the protein when a bond is formed, highlighted by a loss of the maximum emission signal of rAsGFP and a redshift of the maximum absorption peak of the GO/GtO species. Moreover, the hemolysis assay reveals a lower value in the presence of less oxidized graphene species providing evidence for a biocompatible material. This singular aspect can be approached as a promising method for circulating pharmaceutical preparations via intravenous administration in the field of drug delivery.
National Research Council, Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Torretta Granitola, 91021, Trapani, Italy.
Tiziana
T
Masullo
Department of Physics and Chemistry, Viale delle Scienze Ed17, 90128, Palermo, Italy.
Nerina
N
Armata
Department of Physics and Astronomy "Galileo Galilei", University of Padova, Via Marzolo 8, 35131, Padova, Italy.
Flavio
F
Pendolino
National Research Council, Institute of Biomedicine and Molecular Immunology (IBIM), Via Ugo La Malfa, 153, 90146, Palermo, Italy.
Paolo
P
Colombo
Department of Physics and Chemistry, Viale delle Scienze Ed17, 90128, Palermo, Italy.
Fabrizio
F
Lo Celso
National Research Council, Institute for Coastal Marine Environment, Calata Porta di Massa, 80133, Naples, Italy.
Salvatore
S
Mazzola
National Research Council, Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Torretta Granitola, 91021, Trapani, Italy. angela.cuttitta@iamc.cnr.it.
Angela
A
Cuttitta
D001672:Biocompatible Materials; D006108:Graphite / Q000138:chemical synthesis* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
D001672:Biocompatible Materials; D010087:Oxides; D049452:Green Fluorescent Proteins; D006108:Graphite
Biocompatibility; GFP; Graphene oxide; Graphite oxide; Hemolysis; Reduction