Targeted delivery of gold nanoparticle contrast agents for reporting gene detection by magnetic resonance imaging.
Detection of protein expression by MRI requires a high payload of Gd(III) per protein binding event. Presented here is a targeted AuDNA nanoparticle capable of delivering several hundred Gd(III) chelates to the HaloTag reporter protein. Incubating this particle with HaloTag-expressing cells produced a 9.4 contrast-to-noise ratio compared to non-expressing cells.
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA. tmeade@northwestern.edu.
Luke F
LF
Vistain
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA. tmeade@northwestern.edu.
Matthew W
MW
Rotz
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA. tmeade@northwestern.edu.
Richa
R
Rathore
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA. tmeade@northwestern.edu.
Adam T
AT
Preslar
Department of Chemistry, Molecular Biosciences, Neurobiology, Biomedical Engineering, and Radiology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208-3113, USA. tmeade@northwestern.edu.
Thomas J
TJ
Meade
D002460:Cell Line; D002614:Chelating Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D003287:Contrast Media / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004247:DNA / Q000737:chemistry; D005682:Gadolinium / Q000008:administration & dosage* / Q000493:pharmacokinetics; D015870:Gene Expression; D017930:Genes, Reporter; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*
D002614:Chelating Agents; D003287:Contrast Media; D006046:Gold; D004247:DNA; D005682:Gadolinium