Polymorphisms of Tumor Necrosis Factor Alpha in Moroccan Patients with Gastric Pathology: New Single-Nucleotide Polymorphisms in TNF-α(-193) (G/A).
Polymorphisms in tumor necrosis factor alpha (TNF-α) gene are emerging as key determinants of gastric diseases. The TNF-α(-308) (G/A) and TNF-α(-238) (G/A) single-nucleotide polymorphisms SNPs are the most extensively studied. However, all these studies are conducted in Caucasian and Asian populations. Thus, for the first time in Africa, we sought to investigate whether polymorphisms in TNF-α gene were associated with the development of gastric pathology in Morocco. Two SNPs located in the promoter region (positions -308 and -238) in TNF-α gene were genotyped in 244 individuals (170 patients and 74 healthy controls). Odds ratios (ORs) and 95% confidence intervals (CI) were estimated using logistic regression analysis. The TNF-α(-238) (G/A) genotype was significantly associated with a high risk of gastritis and gastric cancer (GC) (P = 0.001 and P = 0.002, resp.). Furthermore, a new polymorphism located in the promoter region at position -193 in TNF-α gene was identified. The distribution of this SNP was markedly different in patients suffering from ulcers. The association between TNF-α(-193) (G/A) genotype and high risk of ulcer was significant (P = 0.03). These results suggest that the TNF-α(-193) (G/A) allele has a protective function against gastric cancer by developing ulcer.
Laboratory of Pathology-Digestif-Oncology, Pasteur Institute of Morocco, 20360 Casablanca, Morocco ; Laboratory of Biology and Health, Molecular Modeling and Quality Control Team, Faculty of Sciences Ben M'sik, University Hassan II, 7955 Casablanca, Morocco.
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Essadik
Department of Radiotherapy Oncology, Ibn Rochd University Hospital, 20000 Casablanca, Morocco.
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Jouhadi
Laboratory of Molecular Genetics and Pathophysiology, Faculty of Sciences Ben M'sik, University Hassan II, 7955 Casablanca, Morocco.
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Rhouda
Laboratory of Pathology-Digestif-Oncology, Pasteur Institute of Morocco, 20360 Casablanca, Morocco.
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Nadifiyine
Laboratory of Biology and Health, Molecular Modeling and Quality Control Team, Faculty of Sciences Ben M'sik, University Hassan II, 7955 Casablanca, Morocco.
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Kettani
Laboratory of Pathology-Digestif-Oncology, Pasteur Institute of Morocco, 20360 Casablanca, Morocco.
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Maachi
D016022:Case-Control Studies; D005756:Gastritis / Q000208:ethnology / Q000235:genetics; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D064368:Healthy Volunteers; D006801:Humans; D009018:Morocco; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012044:Regression Analysis; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics*; D013276:Stomach Ulcer / Q000208:ethnology / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha