miR-125b regulates procalcitonin production in monocytes by targeting Stat3.
Sepsis is a leading cause of mortality in clinical infection, early identification and intervention is critical to improve its outcomes. Nonetheless, it is difficult to determine whether the cause of inflammation in patients is of bacterial origin at early stage. Procalcitonin (PCT) is considered as a promising marker in clinical laboratory test of bacterial infection. But the details for the regulation of PCT expression are not fully understood. miR-125b is important to immune system development and immunological host defense, while its role in the regulation of PCT production remains unexplored. In this study, we demonstrate that the expression of PCT is regulated by miR-125b in human monocytes. miR-125b modulates PCT expression by manipulating the amount and transcriptional activity of Stat3.
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Feng
F
Zhang
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Xueyu
X
Fan
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Yongfeng
Y
Bai
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Jun
J
Lu
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Meixia
M
Zheng
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Juan
J
Chen
Zhejiang Chinese Medical University, No.1 Medical School, Hangzhou, Zhejiang, China.
Yonglin
Y
Liu
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Zhenhong
Z
Chen
Core Facility, Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China. Electronic address: qzhosp@163.com.
Jin
J
Zhu
D002116:Calcitonin / Q000096:biosynthesis*; D015740:Calcitonin Gene-Related Peptide; D002460:Cell Line; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D050796:STAT3 Transcription Factor / Q000378:metabolism*
C000606589:CALCA protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D011498:Protein Precursors; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide
LPS; Monocyte; Procalcitonin; Sepsis; Stat3; miR-125b