The miRNAome of Opisthorchis viverrini induced intrahepatic cholangiocarcinoma.
Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer, arising in the biliary ducts that extend into the liver. The highest incidence of ICC occurs in Southeast Asia, particularly in the Mekong River Basin countries of Thailand, Laos, Cambodia, and Vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke Opisthorchis viverrini (OV), one of only three eukaryote pathogens considered Group one carcinogens. Intrahepatic cholangiocarcinoma is usually diagnosed at an advanced stage, with a poor prognosis and survival often less than 24 months. Hence, biomarkers that enable the early detection of ICC would be desirable and have a potentially important impact on the public health in the resource-poor regions where this cancer is most prevalent. As microRNAs (miRNAs) remain well preserved after formalin fixation, there is much interest in developing them as biomarkers that can be investigated using tumor biopsy samples preserved in formalin fixed paraffin embedded (FFPE) tumor blocks. Recently, we reported the first comprehensive profiling of tissue-based miRNA expression using FFPE from the three most common subtypes of OV-induced ICC tumors: moderately differentiated ICC, papillary ICC, and well-differentiated ICC. We observed that each subtype of OV-induced ICC exhibited a distinct miRNA profile, which suggested the involvement of specific sets of miRNAs in the progression of this cancer. In addition, non-tumor tissue adjacent to ICC tumor tissue on the same FFPE block shared a similar miRNA dysregulation profile with the tumor tissue than with normal (non-tumor) liver tissue (individuals without ICC or OV infection). Herein, we provide a detailed description of the microarray analysis procedures used to derive these findings.
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Jin
J
Peng
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Yanjun
Y
Feng
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Gabriel
G
Rinaldi
Infectious Diseases Program, QIMR Berghofer Medical Research Institute, Brisbane, Australia.
Ponlapat
P
Yonglitthipagon
Department of Pathology, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Samantha E
SE
Easley
Department of Parasitology, Khon Kaen University School of Medicine, Khon Kaen, 40002, Thailand.
Therawach
T
Laha
Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chawalit
C
Pairojkul
Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Vajarabhongsa
V
Bhudhisawasdi
Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Banchob
B
Sripa
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Paul J
PJ
Brindley
Infectious Diseases Program, QIMR Berghofer Medical Research Institute, Brisbane, Australia ; School of Biomedical Sciences, Faculty of Medicine and Biomedical Sciences, University of Queensland, Brisbane, Australia.
Jason P
JP
Mulvenna
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Jeffrey M
JM
Bethony
Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA ; Research Center for the Neglected Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA.
Jordan L
JL
Plieskatt
Cholangiocarcinoma; Intrahepatic cholangiocarcinoma; MicroRNA; Microarray; Opisthorchis viverrini