A Single Biosensor for Evaluating the Levels of Copper Ion and L-Cysteine in a Live Rat Brain with Alzheimer's Disease.
Copper ion (Cu(2+)) and L-cysteine (CySH) are closely correlated with physiological and pathological events of Alzheimer's Disease (AD), however the detailed mechanism is still unclear, mainly owing to a lack of accurate analytical methods in live brains. Herein, we report a single biosensor for electrochemical ratiometric detection of Cu(2+) and CySH in live rat brains with AD. N,N-di-(2-picoly)ethylenediamine (DPEA) is first synthesized for specific recognition of Cu(2+) to form a DPEA-Cu(2+) complex. This complex shows high selectivity for CySH owing to the release of Cu(2+) from the complex through CySH binding to Cu(2+) center. In parallel, 5'-MB-GGCGCGATTTTTTTTTTTTT-SH-3' (HS-DNA-MB, MB=Methylene Blue) is designed as an inner-reference for providing a built-in correction to improve the accuracy. As a result, combined with the amplified effect of Au nanoleaves, our single ratiometric biosensor can be successfully applied in real-time detection of Cu(2+) and CySH in the live rat brains with AD. To our knowledge, this is the first report on the accurate concentrations of Cu(2+) and CySH in live rat brains with AD.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, North Zhongshan Road 3663, Shanghai 200062 (P.R. China).|Xinyu Institute of New Energy, Xinyu University, Sunshine Avenue 2666, Xinyu 338004 (China).
Yongping
Y
Luo
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, North Zhongshan Road 3663, Shanghai 200062 (P.R. China).
Limin
L
Zhang
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, North Zhongshan Road 3663, Shanghai 200062 (P.R. China).
Wei
W
Liu
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou 221004 (China).
Yanyan
Y
Yu
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, North Zhongshan Road 3663, Shanghai 200062 (P.R. China). ytian@chem.ecnu.edu.cn.
Yang
Y
Tian
D000544:Alzheimer Disease / Q000175:diagnosis / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D001923:Brain Chemistry; D003300:Copper / Q000032:analysis*; D003545:Cysteine / Q000032:analysis*; D055664:Electrochemical Techniques; D015394:Molecular Structure; D010316:Particle Size; D051381:Rats; D013499:Surface Properties
D003300:Copper; D003545:Cysteine
Alzheimer’s disease; L-cysteine; biosensors; brain chemistry; copper ions