In Vitro and in Vivo Demonstration of Photodynamic Activity and Cytoplasm Imaging through TPE Nanoparticles.
We synthesized novel tetraphenylethene (TPE) conjugates, which undergo unique self-assembly to form spherical nanoparticles that exhibited aggregation induced emission (AIE) in the near-infrared region. These nanoparticles showed significant singlet oxygen generation efficiency, negligible dark toxicity, rapid cellular uptake, efficient localization in cytoplasm, and high in vitro photocytotoxicity as well as in vivo photodynamic activity against a human prostate tumor animal model. This study demonstrates, for the first time, the power of the self-assembled AIE active tetraphenylethene conjugates in aqueous media as a nanoplatform for future therapeutic applications.
CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST) , Thiruvananthapuram, 695019, India.
Dhanya T
DT
Jayaram
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC) , Barcelona and Biomedical Research Networking Center for Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Zaragoza, 08025, Spain.
Sara
S
Ramos-Romero
CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST) , Thiruvananthapuram, 695019, India.
Balaraman H
BH
Shankar
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC) , Barcelona and Biomedical Research Networking Center for Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Zaragoza, 08025, Spain.
Cristina
C
Garrido
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC) , Barcelona and Biomedical Research Networking Center for Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Zaragoza, 08025, Spain.
Nuria
N
Rubio
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC) , Barcelona and Biomedical Research Networking Center for Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Zaragoza, 08025, Spain.
Lourdes
L
Sanchez-Cid
Grup d'Enginyeria de Materials (GEMAT), Institut Químic de Sarrià, Universitat Ramon Llull , Barcelona 08017, Spain.
Salvador Borros
SB
Gómez
Institute of Advanced Chemistry of Catalonia (IQAC-CSIC) , Barcelona and Biomedical Research Networking Center for Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Zaragoza, 08025, Spain.
Jeronimo
J
Blanco
CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST) , Thiruvananthapuram, 695019, India.|CSIR-North East Institute of Science and Technology (CSIR-NEIST) , Jorhat, 785006, India.
Danaboyina
D
Ramaiah
D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D061848:Optical Imaging; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D011471:Prostatic Neoplasms / Q000628:therapy; D013267:Stilbenes / Q000737:chemistry*
D052160:Benzothiazoles; D017319:Photosensitizing Agents; D013267:Stilbenes