Determination of genotoxic effects of boron and zinc on Zea mays using protein and random amplification of polymorphic DNA analyses.
In this research, we aimed to determine genotoxic effects of boron (B) and zinc (Zn) on Zea mays by using total soluble protein content and random amplification of polymorphic DNA (RAPD) analyses. For the RAPD analysis, 16 RAPD primers were found to produce unique polymorphic band profiles on treated maize seedlings. With increased Zn and B concentrations, increased polymorphism rate was observed, while genomic template stability and total soluble protein content decreased. The treatment with Zn was more effective than that of B groups on the levels of total proteins. The obtained results from this study revealed that the total soluble protein levels and RAPD profiles were performed as endpoints of genotoxicity and these analyses can offer useful biomarker assays for the evaluation of genotoxic effects on Zn and B polluted plants.
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Filiz Aygun
FA
Erturk
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Gokce
G
Nardemir
Department of Biology, Faculty of Science and Arts, University of Ondokuz Mayis, Samsun, Turkey.
A Y
AY
Hilal
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Esra
E
Arslan
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey gagar@atauni.edu.tr.
Guleray
G
Agar
D001895:Boron / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D018744:DNA, Plant / Q000235:genetics; D042822:Genomic Instability / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D036226:Seedlings / Q000187:drug effects; D003313:Zea mays / Q000187:drug effects*; D015032:Zinc / Q000633:toxicity*
D018744:DNA, Plant; D010940:Plant Proteins; D015032:Zinc; D001895:Boron
Boron; RAPD-PCR; genomic template stability; total soluble protein; zinc