Quick and selective extraction of Z-ligustilide from Angelica sinensis using magnetic multiwalled carbon nanotubes.
A facile and highly efficient magnetic solid-phase extraction method has been developed for Z-ligustilide, the major therapeutic agent in Angelica sinensis. The solid-phase adsorbent material used was prepared by conjugating carbon nanotubes with magnetic Fe3 O4 nanoparticles via a hydrothermal reaction. The magnetic material showed a high affinity toward Z-ligustilide due to the π-π stacking interaction between the carbon nanotubes and Z-ligustilide, allowing a quick and selective exaction of Z-ligustilide from complex sample matrices. Factors influencing the magnetic solid-phase extraction such as the amount of the added adsorbent, adsorption and desorption time, and desorption solvent, were investigated. Due to its high extraction efficiency, this method was proved highly useful for sample cleanup/enrichment in quantitative high-performance liquid chromatography analysis. The proposed method had a linear calibration curve (R(2) = 0.9983) over the concentration between 4 ng/mL and 200 μg/mL Z-ligustilide. The accuracy of the method was determined by the recovery, which was from 92.07 to 104.02%, with the relative standard deviations >4.51%.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|Shanghai Institute of Technology, Shanghai, China.
Qiong
Q
Zeng
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|Shanghai Institute of Technology, Shanghai, China.
Yan-Wei
YW
Jia
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA.
Pei-Li
PL
Xu
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|Shanghai Institute of Technology, Shanghai, China.
Meng-Wei
MW
Xiao
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|University of Chinese Academy of Sciences, Beijing, China.
Yi-Ming
YM
Liu
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Shu-Lin
SL
Peng
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
Xun
X
Liao
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D029971:Angelica sinensis / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D015203:Reproducibility of Results; D013997:Time Factors
D037742:Nanotubes, Carbon; D010936:Plant Extracts; C027820:ligustilide; D015107:4-Butyrolactone
Angelica sinensis; Magnetic separation; Multiwalled carbon nanotubes; Solid-phase extraction; Z-ligustilide