Hyaluronidase Activity in Saliva of European Culicoides (Diptera: Ceratopogonidae).
Biting midges of the genus Culicoides transmit pathogens of veterinary importance such as bluetongue virus (Reoviridae: Orbivirus). The saliva of Culicoides is known to contain bioactive molecules including peptides and proteins with vasodilatory and immunomodulative properties. In this study, we detected activity of enzyme hyaluronidase in six Culicoides species that commonly occur in Europe and that are putative vectors of arboviruses. Hyaluronidase was present in all species studied, although its molecular size, sensitivity to SDS, and substrate specificity differed between species. Further studies on the potential effect of hyaluronidase activity on the vector competence of Culicoides species for arboviruses would be beneficial.
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz), Both authors contributed equally to this work, radrova@natur.cuni.cz.
Jana
J
Rádrová
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz), Both authors contributed equally to this work.
Michaela
M
Vlková
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz).
Věra
V
Volfová
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz).
Petra
P
Sumová
Cirad, UMR15 Contrôle des maladies, Montpellier, France (cetre@cirad.fr; ignace.rakotoarivony@cirad.fr; xavier.allene@cirad.fr), INRA, UMR1309 Contrôle des maladies, Montpellier, France.
Catherine
C
Cêtre-Sossah
Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, GU24-0NF, United Kingdom (simon.carpenter@pirbright.ac.uk; karin.darpel@pirbright.ac.uk), and.
Simon
S
Carpenter
Vector-borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Surrey, GU24-0NF, United Kingdom (simon.carpenter@pirbright.ac.uk; karin.darpel@pirbright.ac.uk), and School of Veterinary Medicine, University of Surrey, Guildford GU27AL, United Kingdom.
Karin
K
Darpel
Cirad, UMR15 Contrôle des maladies, Montpellier, France (cetre@cirad.fr; ignace.rakotoarivony@cirad.fr; xavier.allene@cirad.fr), INRA, UMR1309 Contrôle des maladies, Montpellier, France.
Ignace
I
Rakotoarivony
Cirad, UMR15 Contrôle des maladies, Montpellier, France (cetre@cirad.fr; ignace.rakotoarivony@cirad.fr; xavier.allene@cirad.fr), INRA, UMR1309 Contrôle des maladies, Montpellier, France.
Xavier
X
Allène
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz).
Jan
J
Votýpka
Department of Parasitology, Charles University in Prague, Faculty of Science, Czech Republic (radrova@natur.cuni.cz; michaelakindlova84@gmail.com; Veravolf@seznam.cz; sumovapetra@seznam.cz; vapid@natur.cuni.cz; volf@cesnet.cz).
Petr
P
Volf
D000818:Animals; D001102:Arbovirus Infections / Q000635:transmission; D002519:Ceratopogonidae / Q000201:enzymology*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007303:Insect Vectors / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology
D006821:Hyaluronoglucosaminidase
Culicoides; hyaluronidase; saliva