Adaptation of a mixed culture of acidophiles for a tank biooxidation of refractory gold concentrates containing a high concentration of arsenic.
We adapted a mixed culture of acidophiles to high arsenic concentrations to confirm the possibility of achieving more than 70% biooxidation of refractory gold concentrates containing high arsenic (As) concentration. The biooxidation process was applied to refractory gold concentrates containing approximately 139.67 g/kg of total As in a stirred tank reactor using an adapted mixed culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans. The percentage of the biooxidation process was analyzed based on the total As removal efficiency. The As removal was monitored by inductively coupled plasma (ICP) analysis, conducted every 24 h. The results obtained with the adapted culture were compared with the percentage of biooxidation obtained with a non-adapted mixed culture of A. ferrooxidans and A. thiooxidans, and with their respective pure cultures. The percentages of biooxidation obtained during 358 h of reaction were 72.20%, 38.20%, 27.70%, and 11.45% for adapted culture, non-adapted culture, and pure cultures of A. thiooxidans and A. ferrooxidans, respectively. The adapted culture showed a peak maximum percentage of biooxidation of 77% at 120 h of reaction, confirming that it is possible to obtain biooxidation percentages over 70% in gold concentrates containing high As concentrations.
Department of Mineral Resources and Energy Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea.
Jeongsik
J
Hong
Department of Mineral Resources and Energy Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea.
Rene A
RA
Silva
Department of Mineral Resources and Energy Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea.
Jeonghyun
J
Park
Department of Mineral Resources and Energy Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea.
Eunseong
E
Lee
R&D Team, Institute of Mine Reclamation Corporation, Coal Center, 30 Chungjin-dong, Jongno-gu, Seoul 110-727, Republic of Korea.
Jayhyun
J
Park
Department of Mineral Resources and Energy Engineering, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Republic of Korea. Electronic address: kshjkim@jbnu.ac.kr.
Hyunjung
H
Kim
D000064:Acclimatization / Q000187:drug effects*; D042763:Acidithiobacillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D013856:Acidithiobacillus thiooxidans / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D001151:Arsenic / Q000302:isolation & purification* / Q000494:pharmacology*; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects
D006046:Gold; D001151:Arsenic
Acidithiobacillus ferrooxidans; Acidithiobacillus thiooxidans; Gold concentrates; High arsenic concentration; Tank biooxidation