Randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin.
The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB [photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB [PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment.
State University of Campinas, Piracicaba Dental School, Department of Pediatric Dentistry, Av. Limeira, 901, 13414-903 Piracicaba, SP, Brazil.
Carolina
C
Steiner-Oliveira
University of São Paulo, Institute of Biomedical Sciences, Department of Microbiology, Av. Lineu Prestes, 1374-05508-000 São Paulo, SP, Brazil.
Priscila Larcher
PL
Longo
University of São Paulo, School of Dentistry, Special Laboratory of Lasers in Dentistry, Restorative Department, Av. Lineu Prestes, 2227-05508-000 São Paulo, SP, Brazil.
Ana Cecília Corrêa
AC
Aranha
University of São Paulo, School of Dentistry, Special Laboratory of Lasers in Dentistry, Restorative Department, Av. Lineu Prestes, 2227-05508-000 São Paulo, SP, Brazil.
Karen Müller
KM
Ramalho
University of São Paulo, Institute of Biomedical Sciences, Department of Microbiology, Av. Lineu Prestes, 1374-05508-000 São Paulo, SP, Brazil.
Marcia Pinto Alves
MP
Mayer
University of São Paulo, School of Dentistry, Special Laboratory of Lasers in Dentistry, Restorative Department, Av. Lineu Prestes, 2227-05508-000 São Paulo, SP, Brazil.
Carlos
C
de Paula Eduardo
D000890:Anti-Infective Agents / Q000008:administration & dosage*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D003131:Combined Modality Therapy / Q000379:methods; D003731:Dental Caries / Q000175:diagnosis* / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D014094:Tooth, Deciduous / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D016896:Treatment Outcome
D000890:Anti-Infective Agents; D017319:Photosensitizing Agents