Interactions of a biocompatible water-soluble anthracenyl polymer derivative with double-stranded DNA.
We have studied the interaction of a polymeric water soluble anthracenyl derivative () with salmon testes DNA. The results from UV-Vis, fluorescence, Fourier transform infrared (FT-IR) and circular dichroism spectroscopies indicate that the groove binding process regulates the interaction between and DNA. The binding constants, calculated by absorption spectroscopy at 298, 304 and 310 K, were equal to 3.2 × 10(5) M(-1), 4.7 × 10(5) M(-1), and 6.6 × 10(5) M(-1) respectively, proving a relatively high affinity of for salmon testes DNA. Results of Hoechst 33258 displacement assays strongly support the groove binding mode of to DNA. The association stoichiometry of the :DNA adduct was found to be 1 for every 5 base pairs. FT-IR spectra, recorded at different /DNA molar ratios, indicate the involvement of the phosphate groups and adenine and thymine DNA bases in the association process. Thermodynamic results suggest that hydrophobic forces regulate the binding of with DNA without excluding some extent of involvement of van der Waals forces and hydrogen bonding arising due to surface binding between the hydrophilic polymeric arms of the ligand and the functional groups positioned on the edge of the groove. The resulting composite biomaterial could constitute a valuable candidate for future biological and/or photonic applications.
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland. katarzyna.matczyszyn@pwr.edu.pl.
Marco
M
Deiana
Laboratoire de Chimie, CNRS UMR 5182, Ecole Normale Supérieure de Lyon, Université, Lyon 1, Lyon, France.
Bastien
B
Mettra
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland. katarzyna.matczyszyn@pwr.edu.pl.
Katarzyna
K
Matczyszyn
Department of Physical and Quantum Chemistry, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.
Katarzyna
K
Piela
Laboratoire de Chimie, CNRS UMR 5182, Ecole Normale Supérieure de Lyon, Université, Lyon 1, Lyon, France.
Delphine
D
Pitrat
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland. katarzyna.matczyszyn@pwr.edu.pl.
Joanna
J
Olesiak-Banska
Laboratoire de Chimie, CNRS UMR 5182, Ecole Normale Supérieure de Lyon, Université, Lyon 1, Lyon, France.
Cyrille
C
Monnereau
Laboratoire de Chimie, CNRS UMR 5182, Ecole Normale Supérieure de Lyon, Université, Lyon 1, Lyon, France.
Chantal
C
Andraud
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology, Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland. katarzyna.matczyszyn@pwr.edu.pl.
Marek
M
Samoc
D000818:Animals; D000873:Anthracenes / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D008297:Male; D011108:Polymers / Q000737:chemistry*; D012474:Salmon; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013737:Testis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D000873:Anthracenes; D001672:Biocompatible Materials; D011108:Polymers; D014867:Water; D004247:DNA