The brain as immunoprecipitator of serum autoantibodies against N-Methyl-D-aspartate receptor subunit NR1.
Autoantibodies (AB) against N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) are highly seroprevalent in health and disease. Symptomatic relevance may arise upon compromised blood-brain barrier (BBB). However, it remained unknown whether circulating NMDAR1 AB appear in the cerebrospinal fluid (CSF). Of n = 271 subjects with CSF-serum pairs, 26 were NMDAR1 AB seropositive, but only 1 was CSF positive. Contrariwise, tetanus AB (non-brain-binding) were present in serum and CSF of all subjects, with CSF levels higher upon BBB dysfunction. Translational mouse experiments proved the hypothesis that the brain acts as an 'immunoprecipitator'; simultaneous injection of NMDAR1 AB and the non-brain-binding green fluorescent protein AB resulted in high detectability of the former in brain and the latter in CSF.
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Esther
E
Castillo-Gomez
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Anne
A
Kästner
Department of Psychiatry, University of Magdeburg, Magdeburg.
Johann
J
Steiner
German Center for Neurodegenerative Diseases, Göttingen.|Deutsche Forschungsgemeinschaft Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen.
Anja
A
Schneider
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Bilke
B
Hettling
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Giulia
G
Poggi
Department of Psychiatry, University of Magdeburg, Magdeburg.
Kristin
K
Ostehr
Max Planck Institute of Psychiatry, Munich.
Manfred
M
Uhr
Institute for Clinical Chemistry, Göttingen Medical School, Göttingen.
Abdul R
AR
Asif
Department of Neurology, University of Magdeburg, Magdeburg.
Mike
M
Matzke
Max Planck Institute of Psychiatry, Munich.
Ulrike
U
Schmidt
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Viktoria
V
Pfander
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.
Christian
C
Hammer
Institute of Virology, Hannover Medical School, Hannover.
Thomas F
TF
Schulz
Institute for Clinical Chemistry, Göttingen Medical School, Göttingen.
Lutz
L
Binder
Institute for Experimental Immunology, (affiliated with Euroimmun, Lübeck), Göttingen, Germany.
Winfried
W
Stöcker
Max Planck Institute of Psychiatry, Munich.
Frank
F
Weber
Department of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen.|Deutsche Forschungsgemeinschaft Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Göttingen.
Hannelore
H
Ehrenreich
D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000134:cerebrospinal fluid*; D001812:Blood-Brain Barrier / Q000503:physiopathology*; D001921:Brain / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid*; D016194:Receptors, N-Methyl-D-Aspartate / Q000276:immunology*; D016036:Seroepidemiologic Studies
D001323:Autoantibodies; C409466:NR1 NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate