Analysis of the DNA-Binding Activities of the Arabidopsis R2R3-MYB Transcription Factor Family by One-Hybrid Experiments in Yeast.
The control of growth and development of all living organisms is a complex and dynamic process that requires the harmonious expression of numerous genes. Gene expression is mainly controlled by the activity of sequence-specific DNA binding proteins called transcription factors (TFs). Amongst the various classes of eukaryotic TFs, the MYB superfamily is one of the largest and most diverse, and it has considerably expanded in the plant kingdom. R2R3-MYBs have been extensively studied over the last 15 years. However, DNA-binding specificity has been characterized for only a small subset of these proteins. Therefore, one of the remaining challenges is the exhaustive characterization of the DNA-binding specificity of all R2R3-MYB proteins. In this study, we have developed a library of Arabidopsis thaliana R2R3-MYB open reading frames, whose DNA-binding activities were assayed in vivo (yeast one-hybrid experiments) with a pool of selected cis-regulatory elements. Altogether 1904 interactions were assayed leading to the discovery of specific patterns of interactions between the various R2R3-MYB subgroups and their DNA target sequences and to the identification of key features that govern these interactions. The present work provides a comprehensive in vivo analysis of R2R3-MYB binding activities that should help in predicting new DNA motifs and identifying new putative target genes for each member of this very large family of TFs. In a broader perspective, the generated data will help to better understand how TF interact with their target DNA sequences.
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Zsolt
Z
Kelemen
Estación Experimental de Aula Dei/CSIC, Zaragoza, Spain; Fundación ARAID, Zaragoza, Spain.
Alvaro
A
Sebastian
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Wenjia
W
Xu
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Damaris
D
Grain
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Fabien
F
Salsac
Paris-Saclay Institute of Plant Sciences (IPS2), UMR INRA-CNRS-P11-P7-UEVE 1403, Saclay Plant Sciences, Evry, France.
Alexandra
A
Avon
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France; Biochimie et Physiologie Moleculaire des Plantes, UMR 5004, INRA/CNRS/SupAgro-M/UM, Montpellier, France.
Nathalie
N
Berger
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Joseph
J
Tran
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Bertrand
B
Dubreucq
Paris-Saclay Institute of Plant Sciences (IPS2), UMR INRA-CNRS-P11-P7-UEVE 1403, Saclay Plant Sciences, Evry, France.
Claire
C
Lurin
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France.
Loïc
L
Lepiniec
Estación Experimental de Aula Dei/CSIC, Zaragoza, Spain; Fundación ARAID, Zaragoza, Spain.
Bruno
B
Contreras-Moreira
Institut Jean-Pierre Bourgin (IJPB), INRA, AgroParisTech, CNRS, Saclay Plant Sciences, Université Paris-Saclay, Versailles, France; Biochimie et Physiologie Moleculaire des Plantes, UMR 5004, INRA/CNRS/SupAgro-M/UM, Montpellier, France.
Christian
C
Dubos
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D015723:Gene Library; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; C500589:Myb protein, Arabidopsis; D014157:Transcription Factors