Epigallocatechin-3-Gallate Reduces Cytotoxic Effects Caused by Dental Monomers: A Hypothesis.
Resin monomers from dental composite materials leached due to incomplete polymerization or biodegradation may cause contact allergies and damage dental pulp. The cytotoxicity of dental resin monomers is due to a disturbance of intracellular redox equilibrium, characterized by an overproduction of reactive oxygen species (ROS) and depletion of reduced glutathione (GSH). Oxidative stress caused by dental resin monomers leads to the disturbance of vital cell functions and induction of cell apoptosis in affected cells. The nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway plays a key role in the cellular defense system against oxidative and electrophilic stress. Epigallocatechin-3-gallate (EGCG) can activate the Nrf2 pathway and induce expression of a multitude of antioxidants and phase II enzymes that can restore redox homeostasis. Therefore, here, we tested the hypothesis that EGCG-mediated protection against resin monomer cytotoxicity is mediated by activation of the Nrf2 pathway. This study will help to elucidate the mechanism of resin monomer cytotoxicity and provide information that will be helpful in improving the biocompatibility of dental resin materials.
State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).
Yang
Y
Jiao
State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).
Sai
S
Ma
Shaanxi Key Laboratory of Military Stomatology, Xi'an, Shaanxi, China (mainland).
Yirong
Y
Wang
Department of Orthopaedic Oncology, Xijing Hospital Affiliated to The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).
Jing
J
Li
Department of Orthopaedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).
Lequn
L
Shan
State Key Laboratory of Military Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China (mainland).
Jihua
J
Chen
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis; D001672:Biocompatible Materials; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry; D003782:Dental Pulp / Q000187:drug effects; D004285:Dogs; D005978:Glutathione / Q000737:chemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010641:Phenotype; D017382:Reactive Oxygen Species / Q000378:metabolism; D012117:Resins, Synthetic / Q000633:toxicity*; D014070:Tooth / Q000187:drug effects
D000975:Antioxidants; D001672:Biocompatible Materials; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D017382:Reactive Oxygen Species; D012117:Resins, Synthetic; D002392:Catechin; C045651:epigallocatechin gallate; D005978:Glutathione