Clickable Polymeric Coating for Oriented Peptide Immobilization.
A new methodology for the fabrication of an high-performance peptide microarray is reported, combining the higher sensitivity of a layered Si-SiO2 substrate with the oriented immobilization of peptides using a N,N-dimethylacrylamide-based polymeric coating that contains alkyne monomers as functional groups. This clickable polymer allows the oriented attachment of azido-modified peptides via a copper-mediated azide/alkyne cycloaddition. A similar coating that does not contain the alkyne functionality has been used as comparison, to demonstrate the importance of a proper orientation for facilitating the probe recognition and interaction with the target antibody.
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy. laura.sola@icrm.cnr.it.
Laura
L
Sola
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Alessandro
A
Gori
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Marina
M
Cretich
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Chiara
C
Finetti
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Caterina
C
Zilio
Istituto di Chimica del Riconoscimento Molecolare (ICRM), Consiglio Nazionale delle Ricerche (CNR), Via Mario Bianco 9, Milan, 20131, Italy.
Marcella
M
Chiari
D000327:Adsorption; D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D055767:Immobilized Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis; D012821:Silanes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D000480:Alkynes; D001386:Azides; D055767:Immobilized Proteins; D010455:Peptides; D011108:Polymers; D012821:Silanes; D012822:Silicon Dioxide; D012825:Silicon
Click chemistry; Oriented immobilization; Peptide microarray; Polymeric coating