CT Gray-Level Texture Analysis as a Quantitative Imaging Biomarker of Epidermal Growth Factor Receptor Mutation Status in Adenocarcinoma of the Lung.
OBJECTIVE
The purpose of this study was to investigate the radiogenomic correlation between CT gray-level texture features and epidermal growth factor receptor (EGFR) mutation status in adenocarcinoma of the lung.


METHODS
This retrospective study included 25 patients with exon 19 short inframe deletion (exon 19) and 21 patients with exon 21 L858R point (exon 21) EGFR mutations among 125 patients with EGFR mutant adenocarcinoma of the lung. The randomly formed control group consisted of 20 patients selected from 126 patients with EGFR mutation-negative (wild-type) adenocarcinomas. Five gray-level texture features (contrast, correlation, inverse difference moment, angular second moment, and entropy) were analyzed.


RESULTS
Contrast differentiated both exon 19 (p = 0.00027) and exon 21 (p = 0.00001) mutants from the wild type. Wild-type adenocarcinomas had high scores for contrast (mean, 1598.547) compared with EGFR mutants (mean, 679.463). Correlation differentiated both exon 19 (p = 0.017) and exon 21 (p = 0.0015) mutants from wild-type adenocarcinomas. Inverse difference moment differentiated exon 19 mutants from exon 21 mutants (p = 0.019) and both exon 19 (p = 0.044) and exon 21 (p = 0.00001) mutants from wild-type adenocarcinomas. Angular second moment and entropy were not associated with statistically significant differences between mutation statuses.


CONCLUSIONS
Contrast, correlation, and inverse difference moment texture features correlate with EGFR mutation status in adenocarcinoma of the lung. Further investigation with larger prospective studies is needed to validate the role of CT gray-level texture analysis as a quantitative imaging biomarker.
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Efe
E
Ozkan
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Anna
A
West
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Jeffrey A
JA
Dedelow
2 Helen and Harry Gray Cancer Center, Hartford Hospital, Hartford, CT.
Benjamin F
BF
Chu
3 Department of Pathology, The Ohio State University, Columbus, OH.
Weiqiang
W
Zhao
4 Center for Biostatistics, The Ohio State University, Columbus, OH.
Vedat O
VO
Yildiz
5 Department of Internal Medicine, The Ohio State University Comprehensive Cancer Center-Arthur G. James Cancer Hospital, and Richard J. Solove Research Institute, Columbus, OH.
Gregory A
GA
Otterson
3 Department of Pathology, The Ohio State University, Columbus, OH.
Konstantin
K
Shilo
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Subha
S
Ghosh
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Mark
M
King
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Richard D
RD
White
1 Department of Radiology, The Ohio State University, 395 W 12th Ave, Columbus, OH 43210.
Barbaros S
BS
Erdal
D000230:Adenocarcinoma / Q000000981:diagnostic imaging* / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D066246:ErbB Receptors / Q000235:genetics*; D005091:Exons; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D011857:Radiographic Image Interpretation, Computer-Assisted; D012189:Retrospective Studies; D014057:Tomography, X-Ray Computed / Q000379:methods*
D014408:Biomarkers, Tumor; C512478:EGFR protein, human; D066246:ErbB Receptors
CT; adenocarcinoma of the lung; biomarker; epidermal growth factor receptor mutation; quantitative imaging