Thyroid Disruption in Zebrafish Larvae by Short-Term Exposure to Bisphenol AF.
Bisphenol AF (BPAF) is extensively used as a raw material in industry, resulting in its widespread distribution in the aqueous environment. However, the effect of BPAF on the hypothalamic-pituitary-thyroidal (HPT) axis remains unknown. For elucidating the disruptive effects of BPAF on thyroid function and expression of the representative genes along the HPT axis in zebrafish (Danio rerio) embryos, whole-body total 3,3',5-triiodothyronine (TT3), total 3,5,3',5'-tetraiodothyronine (TT4), free 3,3',5-triiodothyronine (FT3) and free 3,5,3',5'-tetraiodothyronine (FT4) levels were examined following 168 h post-fertilization exposure to different BPAF concentrations (0, 5, 50 and 500 μg/L). The results showed that whole-body TT3, TT4, FT3 and FT4 contents decreased significantly with the BPAF treatment, indicating an endocrine disruption of thyroid. The expression of thyroid-stimulating hormone-β and thyroglobulin genes increased after exposing to 50 μg/L BPAF in seven-day-old larvae. The expressions of thyronine deiodinases type 1, type 2 and transthyretin mRNAs were also significantly up-regulated, which were possibly associated with a deterioration of thyroid function. However, slc5a5 gene transcription was significantly down-regulated at 50 μg/L and 500 μg/L BPAF exposure. Furthermore, trα and trβ genes were down-regulated transcriptionally after BPAF exposure. It demonstrates that BPAF exposure triggered thyroid endocrine toxicity by altering the whole-body contents of thyroid hormones and changing the transcription of the genes involved in the HPT axis in zebrafish larvae.
College of Environment and Plant Protection, Hainan University, Haikou 570228, China. ttl-0114@163.com.|School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China. ttl-0114@163.com.
Tianle
T
Tang
College of Environment and Plant Protection, Hainan University, Haikou 570228, China. yangyang_0221@hotmail.com.
Yang
Y
Yang
College of Environment and Plant Protection, Hainan University, Haikou 570228, China. yawenchen2010@hotmail.com.
Yawen
Y
Chen
College of Environment and Plant Protection, Hainan University, Haikou 570228, China. twh1229@163.com.|Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China. twh1229@163.com.
Wenhao
W
Tang
College of Environment and Plant Protection, Hainan University, Haikou 570228, China. wangfuqiang1991@126.com.
Fuqiang
F
Wang
Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China. diaoxip@hainu.edu.cn.
Xiaoping
X
Diao
D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D006706:Homeostasis / Q000187:drug effects; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects; D007453:Iodide Peroxidase / Q000378:metabolism; D007814:Larva / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects*; D013961:Thyroid Gland / Q000187:drug effects*; D013963:Thyroid Hormones / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism; D013974:Thyroxine / Q000378:metabolism; D015027:Zebrafish; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D007453:Iodide Peroxidase; D010636:Phenols; D013963:Thyroid Hormones; D013972:Thyrotropin; D013974:Thyroxine; C109812:iodothyronine deiodinase type I; C583074:bisphenol AF; D005466:Fluorocarbons; D000098826:Bisphenol A Compounds
bisphenol AF (BPAF); hypothalamic-pituitary-thyroid (HPT) axis; thyroid hormones (THs); zebrafish (Danio rerio)