Genome sequence of obligate marine polycyclic aromatic hydrocarbons-degrading bacterium Cycloclasticus sp. 78-ME, isolated from petroleum deposits of the sunken tanker Amoco Milford Haven, Mediterranean Sea.
Cycloclasticus sp. 78-ME isolated from petroleum deposits of the sunken tanker “Amoco Milford Haven” (Gulf of Genoa, Ligurian Sea, Italy) could effectively degrade polycyclic aromatic hydrocarbons of up to five condensed rings. The genome of 78-ME was sequenced and analysed to gain insights into its remarkable degrading capacities. It comprises two circular replicons, the 2,613,078 bp chromosome and the plasmid of 42,347 bp, with 41.84% and 53.28% of the G + C content respectively. A total of 2585 protein-coding genes were obtained, and three large operons with more than fifteen enzymes belonging to four different classes of ring-cleavage dioxygenases were found.
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Enzo
E
Messina
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Renata
R
Denaro
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Francesca
F
Crisafi
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Francesco
F
Smedile
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Simone
S
Cappello
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Maria
M
Genovese
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Lucrezia
L
Genovese
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Laura
L
Giuliano
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy.
Daniela
D
Russo
Institute of Catalysis, CSIC, 28049, Madrid, Spain.
Manuel
M
Ferrer
School of Biological Sciences, Bangor University, Deiniol Road, Bangor, LL57 UW, Gwynedd, United Kingdom.
Peter
P
Golyshin
Institute for Coastal Marine Environment, CNR, 98122 Messina, Italy. Electronic address: michail.yakimov@iamc.cnr.it.
Michail M
MM
Yakimov
D001673:Biodegradation, Environmental; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008522:Mediterranean Sea; D010578:Petroleum / Q000032:analysis / Q000378:metabolism; D044147:Piscirickettsiaceae / Q000235:genetics* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012767:Ships
D010578:Petroleum; D011078:Polychlorinated Biphenyls
Cycloclasticus sp.; Genome sequence; Obligate marine hydrocarbonoclastic bacteria; Polycyclic aromatic hydrocarbons-degrading