CRISPR/Cas9: molecular tool for gene therapy to target genome and epigenome in the treatment of lung cancer.
Although varied drugs and therapies have been developed for lung cancer treatment, in the past 5 years overall survival rates have not improved much. It has also been reported that lung cancer is diagnosed in most of the patients when it is already in the advanced stages with heterogeneous tumors where single therapy is mostly ineffective. A combination of therapies are being administered and specific genes in specific tissues are targeted while protecting normal cell, but most of the therapies face drawbacks for the development of resistance against them and tumor progression. Therefore, therapeutic implications for various therapies need to be complemented by divergent strategies. This review frames utilization of CRISPR/Cas9 for molecular targeted gene therapy leading to long-term repression and activation or inhibition of molecular targets linked to lung cancer, avoiding the cycles of therapy.
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Sachdeva
Padmashree Dr DY Patil Vidyapeeth's Department of Biotechnology and Bioinformatics, Mumbai, Maharashtra, India.
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Sachdeva
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Pal
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Gupta
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Khan
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Majumdar
School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalya, Bhopal, India.
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Tiwari
D000818:Animals; D064130:CRISPR-Associated Proteins / Q000235:genetics / Q000378:metabolism; D064113:CRISPR-Cas Systems / Q000235:genetics; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics; D057890:Epigenomics / Q000379:methods; D018390:Gene Targeting / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D016678:Genome; D023281:Genomics / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000628:therapy*; D017393:RNA Editing
D064130:CRISPR-Associated Proteins