Sulfobetaine-Vinylimidazole Block Copolymers: A Robust Quantum Dot Surface Chemistry Expanding Bioimaging's Horizons.
Long-term inspection of biological phenomena requires probes of elevated intra- and extracellular stability and target biospecificity. The high fluorescence and photostability of quantum dot (QD) nanoparticles contributed to foster their promise as bioimaging tools that could overcome limitations associated with traditional fluorophores. However, QDs' potential as a bioimaging platform relies upon a precise control over the surface chemistry modifications of these nano-objects. Here, a zwitterion-vinylimidazole block copolymer ligand was synthesized, which regroups all anchoring groups in one compact terminal block, while the rest of the chain is endowed with antifouling and bioconjugation moieties. By further application of an oriented bioconjugation approach with whole IgG antibodies, QD nanobioconjugates were obtained that display outstanding intra- and extracellular stability as well as biorecognition capacity. Imaging the internalization and intracellular dynamics of a transmembrane cell receptor, the CB1 brain cannabinoid receptor, both in HEK293 cells and in neurons, illustrates the breadth of potential applications of these nanoprobes.
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Mariana
M
Tasso
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Emerson
E
Giovanelli
Brain Plasticity Unit, ESPCI ParisTech, PSL Research University , CNRS UMR 8249, 10 rue Vauquelin, 75005 Paris, France.
Diana
D
Zala
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Sophie
S
Bouccara
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Alexandra
A
Fragola
SIMM, ESPCI ParisTech, PSL Research University , CNRS UMR 7615, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Mohamed
M
Hanafi
Brain Plasticity Unit, ESPCI ParisTech, PSL Research University , CNRS UMR 8249, 10 rue Vauquelin, 75005 Paris, France.
Zsolt
Z
Lenkei
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Thomas
T
Pons
LPEM, ESPCI ParisTech, PSL Research University , CNRS UMR 8213, Sorbonne Universités, UPMC Univ. Paris 6, 10 rue Vauquelin, 75005 Paris, France.
Nicolas
N
Lequeux
D001622:Betaine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003102:Colloids; D003952:Diagnostic Imaging / Q000379:methods*; D019793:Fluorescein / Q000737:chemistry; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D043884:Receptor, Cannabinoid, CB1 / Q000378:metabolism; D013499:Surface Properties
D003102:Colloids; D007093:Imidazoles; D011108:Polymers; D043884:Receptor, Cannabinoid, CB1; D001622:Betaine; C483727:sulfobetaine; C425704:N-vinylimidazole; D019793:Fluorescein
block copolymers; cannabinoid receptors; cell receptor tracking; oriented immobilization; quantum dots; vinylimidazole; zwitterion