Whole transcriptome microarrays identify long non-coding RNAs associated with cardiac hypertrophy.
Long non-coding RNAs (lncRNAs) have recently emerged as a novel group of non-coding RNAs able to regulate gene expression. While their role in cardiac disease is only starting to be understood, their involvement in cardiac hypertrophy is poorly known. We studied the association between lncRNAs and left ventricular hypertrophy using whole transcriptome microarrays. Wild-type mice and mice overexpressing the adenosine A2A receptor were subjected to transverse aortic constriction (TAC) to induce left ventricular hypertrophy. Expression profiles of lncRNAs in the heart were characterized using genome-wide microarrays. An analytical pipeline was specifically developed to extract lncRNA data from microarrays. We identified 2 lncRNAs up-regulated and 3 lncRNAs down-regulated in the hearts of A2A-receptor overexpressing-mice subjected to TAC compared to wild-type mice. Differential expression of these 2 lncRNAs was validated by quantitative PCR. Complete microarray dataset is available at Gene Expression Omnibus (GEO) database (http://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE45423. Here, we describe in details the experimental design, microarray performance and analysis.
Laboratory of Cardiovascular Research, Luxembourg Institute of Health, Luxembourg.
Lu
L
Zhang
Department of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA, United States.
Eman A
EA
Hamad
Laboratory of Cardiovascular Research, Luxembourg Institute of Health, Luxembourg.
Mélanie
M
Vausort
Department of Cardiology, Fukuoka National Hospital, Fukuoka, Japan.
Hajime
H
Funakoshi
Genomics Research Unit, Luxembourg Institute of Health, Luxembourg.
Nathalie
N
Nicot
Genomics Research Unit, Luxembourg Institute of Health, Luxembourg.
Petr V
PV
Nazarov
Genomics Research Unit, Luxembourg Institute of Health, Luxembourg.
Laurent
L
Vallar
Department of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA, United States.
Arthur M
AM
Feldman
Laboratory of Cardiovascular Research, Luxembourg Institute of Health, Luxembourg ; Division of Cardiology, Centre Hospitalier, Luxembourg.
Daniel R
DR
Wagner
Laboratory of Cardiovascular Research, Luxembourg Institute of Health, Luxembourg.
Yvan
Y
Devaux
Adenosine A2a receptor; Long non-coding RNA; Microarray; Transverse aortic constriction