Arabidopsis KLU homologue GmCYP78A72 regulates seed size in soybean.
Soybean (Glycine max) is one of the most important crops in the world, and its yield is largely determined by grain weight and grain size. However, the genes that regulate soybean seed size have not been identified. CYP78A, which is highly conserved within terrestrial plants, regulates organ development. In Arabidopsis, AtCYP78A5/KLU has been shown to determine seed size. In the present study, soybean CYP78A72 (GmCYP78A72), one of the orthologs of KLU, was over-expressed in both Arabidopsis and soybean to examine its function in plant development. GmCYP78A72 heterologous expression in Arabidopsis resulted in enlarged sepals, petals, seeds and carpel. Over-expression of GmCYP78A72 in soybean resulted in increased pea size, which is an extremely desirable trait for enhancing productivity. Moreover, knock-down of GmCYP78A72 does not reduce grain size. However, silencing of GmCYP78A57, GmCYP78A70 and GmCYP78A72 genes in triplet reduces the seed size significantly indicating functional redundancy of these three GmCYP78A genes. In conclusion, we investigated the role of CYP78A in soybean seed regulation, and our strategy can be effectively used to engineer large seed traits in soybean varieties as well as other crops.
College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, People's Republic of China.|Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 4888 Shengbei Street, Changchun, 130102, Jilin, People's Republic of China.
Baotian
B
Zhao
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 4888 Shengbei Street, Changchun, 130102, Jilin, People's Republic of China.
Aihua
A
Dai
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 4888 Shengbei Street, Changchun, 130102, Jilin, People's Republic of China.
Haichao
H
Wei
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 4888 Shengbei Street, Changchun, 130102, Jilin, People's Republic of China. yangsuxin@iga.ac.cn.
Suxin
S
Yang
College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, People's Republic of China.
Baoshan
B
Wang
Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA.
Ning
N
Jiang
College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, People's Republic of China. fengxz@neigaehrb.ac.cn.|Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 4888 Shengbei Street, Changchun, 130102, Jilin, People's Republic of China. fengxz@neigaehrb.ac.cn.
Xianzhong
X
Feng
D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D013025:Glycine max / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D029681:Arabidopsis Proteins; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System; C546334:KLUH protein, Arabidopsis
CYP78A; GmCYP78A72; Seed size; Soybean