A Novel Optical Biosensing System Using Mach-Zehnder-Type Optical Waveguide for Influenza Virus Detection.
In order to minimize the damage from viral epidemics, early detection of the causative agent of a viral epidemic and prevention of its immediate spread are urgent social demands. Therefore, in this study, we evaluated the utility of a Mach-Zehnder-type optical waveguide as a sensing device for influenza virus detection. However, it is impossible to detect a 100-nm-size virus using a sol-gel optical biosensor because sol-gel glass has a pore size of only a few nanometers, which makes it impossible for the virus to diffuse into the silica thin film. In order to construct the influenza-specific Mach-Zehnder optical biosensor for influenza detection, a stable antibody immobilization method with resulting high density on the sol-gel surface is strongly required. In this study, the sol-gel glass surface was modified with amino and carboxyl groups, and an anti-H1N1/HA1 antibody was covalently immobilized using a cross-linking agent. We successfully prepared a carboxyl-modified sol-gel surface, using NHS/EDC as the cross-linker, for antibody immobilization, and confirmed the detection of influenza virus using the antibody-immobilized sol-gel glass. After treatment with a 100 μg/mL influenza virus solution for 15 min, a peak wavelength shift (~24 nm) was observed in the output light spectrum.
Tenure-Track Program for Innovation Research, University of Fukui, Fukui, Japan.|Research and Education Program for Life Science, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan.
Hiroaki
H
Sakamoto
Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan.
Yuma
Y
Minpou
Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan.|Research and Education Program for Life Science, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan.
Takayuki
T
Sawai
Optoelectonic Engineering, School of Systems Engineering, Kochi University of Technology, Kochi, Japan.
Yasufumi
Y
Enami
Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan. suyeb10@u-fukui.ac.jp.|Research and Education Program for Life Science, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan. suyeb10@u-fukui.ac.jp.
Shin-ichiro
S
Suye
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D055095:Optics and Photonics / Q000295:instrumentation*
Influenza virus detection; Molecular immobilization; Optical biosensing