Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans.
In this work, a two-step process was developed for the production of 3-hydroxypropionic acid from glycerol. In the first step, glycerol was converted to 1,3-propanediol by Klebsiella pneumonia. In the second step, the 1,3-propanediol was converted into 3-hydroxypropionic acid by Gluconobacter oxydans. In a 7.0 L bioreactor, the whole process took 54 h, consumed 480 g glycerol and produced 242 g 3-hydroxypropionic acid. The conversion rate of glycerol to 3-hydroxypropionic acid was 50.4 % (g g(-1)). The final concentration of 3-hydroxypropionic acid arrived 60.5 g L(-1). The process was effective for 3-HP production from glycerol and it might provide a new approach to the biosynthesis of 3-HP from a cheap starting material. Moreover, in this paper, it was first reported that the by-product of 3-hydroxypropionic acid production from 1,3-propandeiol was acrylic acid.
Li
L
Zhao
Jinping
J
Lin
Hualei
H
Wang
Jingli
J
Xie
Dongzhi
D
Wei
D019149:Bioreactors; D020600:Gluconobacter oxydans / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
D019344:Lactic Acid; C031601:hydracrylic acid; D005990:Glycerol