[Effect of Kv1.3 and KCa3.1 potassium ion channels on the proliferation and migration of monocytes/macrophages].
This study was aimed to investigate the effects of blockade of Ca(2+) activated channel KCa3.1 and voltage-gated potassium channel Kv1.3 of the monocytes/macrophages on inflammatory monocyte chemotaxis. Chemotaxis assay was used to test the inflammatory Ly-6C(hi) monocyte chemotaxis caused by the monocytes/macrophages. The proliferation of monocytes/macrophages was detected by cell counting kit-8 (CCK8). Enzyme-linked immunosorbent assay (ELISA) was applied to detect the C-C motif ligand 7 (CCL7) in cultured media. The results showed that the recruitment of Ly-6C(hi) monocyte induced by monocytes/macrophages was suppressed by the potent Kv1.3 blocker Stichodactyla helianthus neurotoxin (ShK) or the specific KCa3.1 inhibitor TRAM-34. Meanwhile, the proliferation of monocytes/macrophages was significantly inhibited by ShK. The response of Ly-6C(hi) monocyte pretreated with ShK or TRAM-34 to CCL2 was declined. These results suggest that KCa3.1 and Kv1.3 may play an important role in monocytes/macrophages' proliferation and migration.
Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
Shuang-Xia
SX
Zhang
Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
Xian-Pei
XP
Wang
Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China. chuanyu.gao@yahoo.com.
Chuan-Yu
CY
Gao
Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
Chen-Hui
CH
Ju
Department of Cardiology, Zhengzhou University People's Hospital, Zhengzhou 450003, China.
Li-Jie
LJ
Zhu
Research Center of Ion Channelopathy, Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China.
Yi-Mei
YM
DU
D002465:Cell Movement; D049109:Cell Proliferation; D003064:Cnidarian Venoms / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051664:Kv1.3 Potassium Channel / Q000037:antagonists & inhibitors / Q000502:physiology*; D008264:Macrophages / Q000166:cytology*; D009000:Monocytes / Q000166:cytology*; D017434:Protein Structure, Tertiary; D011720:Pyrazoles / Q000494:pharmacology; D051640:Small-Conductance Calcium-Activated Potassium Channels / Q000037:antagonists & inhibitors / Q000502:physiology*
D003064:Cnidarian Venoms; C406328:KCNN3 protein, human; D051664:Kv1.3 Potassium Channel; D011720:Pyrazoles; D051640:Small-Conductance Calcium-Activated Potassium Channels; C411671:TRAM 34; C059713:neurotoxin I, Stichodactyla helianthus