Stimulation of the Drosophila immune system alters genome-wide nucleosome occupancy.
In eukaryotes, nucleosomes participate in all DNA-templated events by regulating access to the underlying DNA sequence. However, nucleosome dynamics during a genome response have not been well characterized [1,2]. We stimulated Drosophila S2 cells with heat-killed Gram-negative bacteria Salmonella typhimurium, and mapped genome-wide nucleosome occupancy at high temporal resolution by MNase-seq using Illumina HiSeq 2500. We show widespread nucleosome occupancy change in S2 cells during the immune response, with the significant nucleosomal loss occurring at 4 h after stimulation. Data have been deposited to the Gene Expression Omnibus (GEO) database repository with the dataset identifier GSE64507.
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Yingxue
Y
Ren
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Daniel L
DL
Vera
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Kimberly A
KA
Hughes
Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.
Jonathan H
JH
Dennis
Drosophila; Illumina Hi-Seq; MNase; Nucleosome; Salmonella