When the Label Matters: Adsorption of Labeled and Unlabeled Proteins on Charged Surfaces.
Fluorescent labels are often attached to proteins to monitor binding and adsorption processes. Docking simulations for native hen egg white lysozyme (HEWL) and HEWL labeled with fluorescein isothiocyanate show that these adsorb differently on charged surfaces. Attachment of even a small label can significantly change the interaction properties of a protein. Thus, the results of experiments with fluorescently labeled proteins should be interpreted by modeling the structures and computing the interaction properties of both labeled and unlabeled species.
Molecular and Cellular Modeling Group, Heidelberg Institute for Theoretical Studies , 69117 Heidelberg, Germany.
Julia
J
Romanowska
Molecular and Cellular Modeling Group, Heidelberg Institute for Theoretical Studies , 69117 Heidelberg, Germany.
Daria B
DB
Kokh
Molecular and Cellular Modeling Group, Heidelberg Institute for Theoretical Studies , 69117 Heidelberg, Germany.|Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance, Heidelberg University , 69117 Heidelberg, Germany.|Interdisciplinary Center for Scientific Computing (IWR), Heidelberg University , 69117 Heidelberg, Germany.
Rebecca C
RC
Wade
D000327:Adsorption; D000818:Animals; D002645:Chickens; D005456:Fluorescent Dyes / Q000737:chemistry*; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D009113:Muramidase / Q000737:chemistry*; D013194:Staining and Labeling; D013499:Surface Properties
D005456:Fluorescent Dyes; C421864:hen egg lysozyme; D009113:Muramidase
Brownian dynamics; fluorescent label; molecular electrostatic potential; protein adsorption; protein labeling; protein−surface interactions