Supporting data of spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo.
This data article contains seven figures and two tables supporting the research article entitled: spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo[1]. The data explain the culture of stromal cells in vitro in three culture systems: discs, scaffolds and scaffolds in a perfusion bioreactor system. Also, quantification of extracellular matrix components (ECM) in vitro and staining of ECM components in vivo can be found here. Finally the quantification of blood vessels dimensions from CD31 signals and representative histograms of stanniocalcin-1 fluorescent signals in negative controls and experimental conditions in vivo are presented.
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
Gustavo A
GA
Higuera
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
Hugo
H
Fernandes
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
Tim W G M
TW
Spitters
Erasmus Medical Center, Internal Medicine, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands.
Jeroen
J
van de Peppel
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
Nils
N
Aufferman
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
Roman
R
Truckenmueller
Biophysical Engineering Group, Mesa Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
Maryana
M
Escalante
TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, The Netherlands.
Reinout
R
Stoop
Erasmus Medical Center, Internal Medicine, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands.
Johannes P
JP
van Leeuwen
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of cell biology inspired tissue engineering, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
Jan
J
de Boer
Biophysical Engineering Group, Mesa Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
Vinod
V
Subramaniam
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
Marcel
M
Karperien
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
Clemens
C
van Blitterswijk
Systems and Control Group, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands.
Anton
A
van Boxtel
Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
Lorenzo
L
Moroni