Cell Membrane Perforation with Photosensitizer and a Brush-shaped Soft-polymer Sheet Using a Malignant Glioma Cell Line.
BACKGROUND
Transduction of foreign molecules into cells is an important technique to investigate the functions of corresponding molecules and/or targets. Recently, a mass-producible nanoprinting perforator was devised enabling for large-scale, high-performance drug or nucleic-acid transfer into cells without cell damage. Since little is known on the performance of the system, we investigated its effects on a malignant glioma cell line.


METHODS
Photosensitization was performed by the Cell Stamper CP-01. The malignant U373MG glioma cell line was used for transduction.


RESULTS
Photosensitization transduced FITC-conjugated albumin into cells. Trypan blue inclusion test demonstrated membrane disintegration by the procedure and scanning electron microscopy disclosed perforation of the cell membrane.


CONCLUSIONS
Local oxidation reaction during the nanoprinting caused reversible membrane perforation. Morphological findings from the current study support the above mechanism, therefore the specific printing system might be convenient for transduction of foreign molecules into malignant glioma cells.
Division of Molecular Cell Biology, Core Research Facilities for Basic Science, Jikei University School of Medicine, Tokyo, Japan.
Toshiaki
T
Tachibana
Department of Neurosurgery, Jikei University School of Medicine, Tokyo, Japan.
Yuta
Y
Suzuki
Division of Molecular Cell Biology, Core Research Facilities for Basic Science, Jikei University School of Medicine, Tokyo, Japan.
Kouki
K
Fujioka
Division of Molecular Cell Biology, Core Research Facilities for Basic Science, Jikei University School of Medicine, Tokyo, Japan.
Keiichi
K
Ikeda
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Yuriko
Y
Inoue
Research Institute of Bio System Information, Tohoku Chemical Co., Ltd., Iwate, Japan.
Yoshifumi
Y
Tada
Department of Machine Intelligence and Systems Engineering, Akita Prefectural University, Yurihonjo City, Akita, Japan.
Takashi Kei
TK
Saito
Division of Molecular Cell Biology, Core Research Facilities for Basic Science, Jikei University School of Medicine, Tokyo, Japan manome@jikei.ac.jp.
Yoshinobu
Y
Manome
D000418:Albumins / Q000008:administration & dosage; D002462:Cell Membrane / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D005910:Glioma / Q000188:drug therapy / Q000473:pathology*; D006801:Humans; D008027:Light; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry*; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000418:Albumins; D017319:Photosensitizing Agents; D011108:Polymers
Cell stamper; malignant glioma; nanoprinting perforator