[HPLC Characteristic Fingerprint of "Boju" Chrysanthemum morifolium].
OBJECTIVE
To provide a scientific basis for quality control of "Boju" Chrysanthemum morifolium by establishing a HPLC characteristic fingerprint.


METHODS
The HPLC analysis was performed on a Spursil C18 chromatographic column (250 mm x 4. 6 mm, 5 µm), and the mobile phase was acetonitrile-0. 1% phosphoric acid in a gradient mode with the flow rate of 1. 0 mL/min. The detection wavelength was 325 nm and the temperature of column was 30 °C.


RESULTS
The common pattern of HPLC characteristic chromatographic profile was established. There were 16 common peaks, five of which were identified in the pattern. The similarities of 10 batches of "Boju" Chrysanthemum monrfolium were evaluated, and all of them were greater than 0. 900.


CONCLUSIONS
This analysis method of HPLC characteristic chromatographic fingerprint is simple and reproducible, and it can provide a scientific basis for identification and quality evaluation of "Boju" Chrysanthemum monfolium.
Nian-jun
NJ
Yu
Jiao
J
Yu
Wei
W
Zheng
Yong
Y
Cao
Tai-hua
TH
Zheng
Yun-qin
YQ
Wang
D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011786:Quality Control
D004365:Drugs, Chinese Herbal