The Effect of Platelet-rich Fibrin Matrix on Rotator Cuff Healing in a Rat Model.
The purpose of the current study was to determine if the application of platelet-rich fibrin matrix could improve regeneration of the tendon-bone insertion site in a rat rotator cuff repair model. 25 Lewis syngeneic rats underwent bilateral tenotomy and repair of the supraspinatus tendon. 10 separate rats were used for PRFM harvest. All left (control) shoulders underwent transosseous rotator cuff repair, while all right (treatment) shoulders were repaired similarly with PRFM augmentation. 9 rats were sacrificed at 2-weeks and ten at 4-weeks for biomechanical testing. 3 separate rats were sacrificed at 2-weeks and 4-weeks each for histologic analysis of the insertion site. At 2 weeks, the experimental group repairs were significantly stronger in ultimate load to failure (P=0.01), stress (P=0.03), and stiffness (P=0.03). Differences in biomechanical testing were not found between the groups at 4 weeks. Histological analysis revealed less collagen organization and cartilage formation at the insertion site in the experimental group. Semiquantitative histologic analysis confirmed our qualitative assessment of the specimens. PRFM does not recapitulate the native enthesis, but rather induces an exuberant and disordered healing response that is characterized by fibrovascular scar tissue.
Orthopaedic Surgery, NYU Hospital for Joint Diseases, New York, United States.
S
S
Hasan
Orthopaedic Surgery, NYU Hospital for Joint Diseases, New York, United States.
M
M
Weinberg
Orthopaedic Surgery, NYU Hospital for Joint Diseases, New York, United States.
O
O
Khatib
Orthopaedic Surgery, New York University Hospital for Joint Diseases, New York, United States.
L
L
Jazrawi
Orthopaedic Surgery, New York University Hospital for Joint Diseases, New York, United States.
E J
EJ
Strauss
D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000378:metabolism; D005337:Fibrin / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D053657:Platelet-Rich Plasma; D011917:Rats, Inbred Lew; D017006:Rotator Cuff / Q000473:pathology / Q000502:physiology / Q000601:surgery; D000070636:Rotator Cuff Injuries; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D005337:Fibrin; D003094:Collagen