Impact of OAS1 Exon 7 rs10774671 Genetic Variation on Liver Fibrosis Progression in Egyptian HCV Genotype 4 Patients.
The aim of this study was to assess the impact of genetic variants of oligoadenylate synthetase 1 (OAS1) single-nucleotide polymorphism (SNP) rs10774671 at the exon 7 splice acceptor site on liver fibrosis progression and hepatitis C virus (HCV) outcome in Egyptian HCV genotype 4 patients. In this study, 195 subjects were enrolled; 60 controls and 135 chronic HCV genotype 4 patients with different fibrosis grades. All subjects were genotyped for OAS1 SNP rs10774671 polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. There was an increasing trend of liver fibrosis progression as 52.9% GG, 73.6% GA, and 83.3% AA genotypes were detected in late fibrosis patients (p = 0.025). The AA genotype was higher in the late fibrosis group than in the early fibrosis group (83.3% vs. 16.7%) (p = 0.001). The A allele was significantly affecting the liver fibrosis progression rate, more than the G allele (p = 0.001). The multivariate analysis showed that the OAS1 GA and AA genotypes were independent factors associated with liver progression (p = 0.009, odds ratio [OR] 3.467, 95% confidence interval [CI] 1.273-7.584). In addition, the A allele was associated with liver fibrosis progression (p = 0.014, OR 2.525, 95% CI 1.157-4.545). The polymorphism at OAS1 exon 7 rs3741981 might be a potential genetic marker and can be useful in the assessment of liver fibrosis progression and disease outcome in HCV-infected patients.
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Noha G
NG
Bader El Din
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Mohamed A
MA
Anany
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Reham M
RM
Dawood
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Marwa K
MK
Ibrahim
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Reem
R
El-Shenawy
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Yasmin S
YS
El Abd
Department of Microbial Biotechnology, National Research Centre , Giza, Egypt .
Mostafa K
MK
El Awady
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000328:Adult; D000368:Aged; D000483:Alleles; D018450:Disease Progression; D004534:Egypt; D005091:Exons; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019698:Hepatitis C, Chronic / Q000150:complications* / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis
C475085:OAS1 protein, human; D015088:2',5'-Oligoadenylate Synthetase