Growth and Pathogenic Potential of Naturally Selected Reassortants after Coinfection with Pandemic H1N1 and Highly Pathogenic Avian Influenza H5N1 Viruses.
Coinfection of ferrets with H5N1 and pH1N1 viruses resulted in two predominate genotypes in the lungs containing surface genes of highly pathogenic avian influenza H5N1 virus in the backbone of pandemic H1N1 2009 (pH1N1). Compared to parental strains, these reassortants exhibited increased growth and virulence in vitro and in mice but failed to be transmitted indirectly to naive contact ferrets. Thus, this demonstrates a possible natural reassortment following coinfection as well as the pathogenicity of the potential reassortants.
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Min-Suk
MS
Song
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Yun Hee
YH
Baek
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Philippe Noriel Q
PN
Pascua
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Hyeok-Il
HI
Kwon
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Eun-Ha
EH
Kim
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Su-Jin
SJ
Park
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Se Mi
SM
Kim
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Young-Il
YI
Kim
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Won-Suk
WS
Choi
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea.
Eung-Gook
EG
Kim
College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
Chul-Joong
CJ
Kim
College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea choiki55@chungbuk.ac.kr.
Young Ki
YK
Choi
D000818:Animals; D060085:Coinfection / Q000635:transmission / Q000821:virology*; D004195:Disease Models, Animal; D018562:Disease Transmission, Infectious; D005289:Ferrets; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000254:growth & development; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000254:growth & development; D008168:Lung / Q000821:virology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000635:transmission / Q000821:virology*; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification*; D014774:Virulence