Contrasting Effects of Long-Term Grazing and Clipping on Plant Morphological Plasticity: Evidence from a Rhizomatous Grass.
Understanding the mechanism of plant morphological plasticity in response to grazing and clipping of semiarid grassland can provide insight into the process of disturbance-induced decline in grassland productivity. In recent studies there has been controversy regarding two hypotheses: 1) grazing avoidance; and 2) growth limiting mechanisms of morphological plasticity in response to defoliation. However, the experimental evidence presented for the memory response to grazing and clipping of plants has been poorly reported. This paper reports on two experiments that tested these hypotheses in field and in a controlled environment, respectively. We examined the effects of long-term clipping and grazing on the functional traits and their plasticity for Leymus chinensis (Trin.) Tzvelev (the dominate species) in the typical-steppe grassland of Inner Mongolia, China. There were four main findings from these experiments. (i) The majority of phenotypic traits of L. chinensis tended to significantly miniaturize in response to long-term field clipping and grazing. (ii) The significant response of morphological plasticity with and without grazing was maintained in a hydroponic experiment designed to remove environmental variability, but there was no significant difference in L. chinensis individual size traits for the clipping comparison. (iii) Plasticity indexes of L. chinensis traits in a controlled environment were significantly lower than under field conditions indicating that plants had partial and slight memory effect to long-term grazing. (iv) The allometry of various phenotypic traits, indicated significant trade-offs between leaf and stem allocation with variations in plant size induced by defoliation, which were maintained only under grazing in the hydroponic controlled environment experiment. Taken together, our findings suggest that the morphological plasticity of L. chinensis induced by artificial clipping was different with that by livestock grazing. The miniaturization of plant size in long-term grazed grassland may reflect retained characteristics of dwarf memory for adaptation to long-term grazing by large herbivores.
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Xiliang
X
Li
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Zinian
Z
Wu
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Zhiying
Z
Liu
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Xiangyang
X
Hou
New South Wales Department of Primary Industries, Orange Agricultural Institute, Orange, New South Wales, 2800, Australia.
Warwick
W
Badgery
College of Life sciences, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010010, P.R. China.
Huiqin
H
Guo
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Qingshan
Q
Zhao
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Ningning
N
Hu
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Junjie
J
Duan
National Forage Improvement Center, Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, Inner Mongolia, 010010, P.R. China.
Weibo
W
Ren
D000818:Animals; D002681:China; D004780:Environment, Controlled; D060434:Herbivory / Q000502:physiology; D063245:Plant Development; D018515:Plant Leaves / Q000254:growth & development*; D006109:Poaceae / Q000254:growth & development*; D027343:Rhizome / Q000254:growth & development