Morphology of sealant/enamel interface after surface treatment with bioactive glass.
The purpose of this study was to analyze, by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), the morphology of sealant/enamel interface after surface treatment with Biosilicate. Before pits and fissures sealing, the occlusal surfaces of 10 sound human molars were sectioned perpendicularly at the fissures in order to obtain three slices for each tooth. Slices were randomly assigned into three groups (n = 10) according to sealing protocol: Group 1- Acid etching + Biosilicate + glass ionomer-based sealant (Clinpro XT Varnish, 3M ESPE); Group 2- Acid etching + glass ionomer-based sealant (Clinpro XT Varnish, 3M ESPE); Group 3- No sealing. All slices were subjected to thermal cycling (5,000 cycles; 5-55°C; dwell time: 30s). Half of the slices from each group (n = 5) were analyzed by CLSM and the other half by SEM. Groups 1 and 2 were also submitted to EDS analysis and their data were evaluated by Two-Way ANOVA e Tukey's test (α=5%). EDS data analysis showed higher amounts of silicon (Si) ions than calcium (Ca) ions in Group 1 (P < 0.05); Group 2 presented higher amounts (P < 0.05) of Ca ions than Si ions. It may be concluded that the use of Biosilicate for surface treatment did not affect the morphology of glass ionomer-based sealant/enamel interfaces.
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av. Do Café S/N, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Fernanda de Carvalho
Fde C
Panzeri Pires-de-Souza
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av. Do Café S/N, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Renata Espíndola
RE
Silveira
Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas, Av. Limeira, 901, CEP: 13414-903, Caixa Postal 52, Piracicaba, São Paulo, Brazil.
Gabriel
G
Abuna
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av. Do Café S/N, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Michelle Alexandra
MA
Chinelatti
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av. Do Café S/N, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Carla Cecilia
CC
Alandia-Román
Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas, Av. Limeira, 901, CEP: 13414-903, Caixa Postal 52, Piracicaba, São Paulo, Brazil.
Mario Alexandre Coelho
MA
Sinhoreti
D003743:Dental Enamel / Q000648:ultrastructure*; D045662:Dental Etching; D005898:Glass; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000648:ultrastructure; D010895:Pit and Fissure Sealants / Q000032:analysis*; D013499:Surface Properties; D013696:Temperature
D010895:Pit and Fissure Sealants; C120461:sodium oxide-calcium oxide-silicon dioxide-diphosphorus pentoxide
bioactive glass; dental materials; dental sealants; tooth remineralization