Niabella ginsenosidivorans sp. nov., isolated from compost.
A Gram-reaction negative, strictly aerobic, non-motile, orange colored, and rod-shaped bacterium (designated BS26(T)) isolated from compost, was characterized by a polyphasic approach to clarify its taxonomic position. Strain BS26(T) was observed to grow optimally at 25-30 °C and at pH 7.0 on R2A and nutrient media. Strain BS26(T) showed ß-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the active components of ginseng) to ginsenoside compound-K (C-K). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BS26(T) belongs to the genus Niabella of family Chitinophagaceae and was most closely related to Niabella soli DSM 19437(T) (94.5% similarity), N. yanshanensis CCBAU 05354(T) (94.3%), and N. aurantiaca DSM 17617(T) (93.8%). The G+C content of genomic DNA was 47.3 mol%. Chemotaxonomic data [predominant isoprenoid quinone-MK-7, major fatty acids-iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH, and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c)] supported the affiliation of strain BS26(T) to the genus Niabella. However, strain BS26(T) could be differentiated genotypically and phenotypically from the recognized species of the genus Niabella. The novel isolate therefore represents a novel species, for which the name Niabella ginsenosidivorans sp. nov. is proposed, with the type strain BS26(T) (=KACC 16620(T) =JCM 18199(T)).
Department of Animal Science and Technology, Konkuk University, Seoul, 05029, Republic of Korea.
Kwon-Jung
KJ
Yi
Department of Biotechnology, Hankyoung National University, Anseong, 17579, Republic of Korea.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, Anseong, 17579, Republic of Korea.
Wan-Taek
WT
Im
National Institute of Animal Science, RDA, Wanju, 55365, Republic of Korea.
Dong-Woon
DW
Kim
Department of Biotechnology, Hankyoung National University, Anseong, 17579, Republic of Korea.|Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyoung National University, Anseong, 17579, Republic of Korea.
Qing Mei
QM
Liu
Department of Animal Science and Technology, Konkuk University, Seoul, 05029, Republic of Korea. sookikim@konkuk.ac.kr.
Soo-Ki
SK
Kim
D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial; D005227:Fatty Acids / Q000737:chemistry; D005838:Genotype; D036145:Ginsenosides / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D036145:Ginsenosides; D012336:RNA, Ribosomal, 16S; D012987:Soil; C442759:ginsenoside Rb1; C112772:ginsenoside compound K
16S rRNA gene sequence; Niabella ginsenosidivorans; compost; decayed feedstuff; ginsenoside; polyphasic taxonomy