The effects of a thermophile metabolite, tryptophol, upon protecting shrimp against white spot syndrome virus.
White spot syndrome virus (WSSV) is a shrimp pathogen responsible for significant economic loss in commercial shrimp farms and until now, there has been no effective approach to control this disease. In this study, tryptophol (indole-3-ethanol) was identified as a metabolite involved in bacteriophage-thermophile interactions. The dietary addition of tryptophol reduced the mortality in shrimp Marsupenaeus japonicus when orally challenged with WSSV. Our results revealed that 50 mg/kg tryptophol has a better protective effect in shrimp than 10 or 100 mg/kg tryptophol. WSSV copies in shrimp were reduced significantly (P < 0.01) when supplemented with 50 mg/kg tryptophol, indicating that virus replication was inhibited by tryptophol. Consequently, tryptophol represents an effective antiviral dietary supplement for shrimp, and thus holds significant promise as a novel and efficient therapeutic approach to control WSSV in shrimp aquaculture.
College of Animal Science and Technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China. Electronic address: zhufei@zju.edu.cn.
Fei
F
Zhu
State Key Laboratory Breeding Base of Marine Genetic Resource, Third Institute of Oceanography, SOA, Xiamen 361005, China.
Min
M
Jin
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D017756:Aquaculture; D001435:Bacteriophages / Q000187:drug effects* / Q000502:physiology; D019587:Dietary Supplements / Q000032:analysis; D056506:Geobacillus / Q000378:metabolism / Q000821:virology*; D007211:Indoles / Q000494:pharmacology*; D055442:Metabolome; D033561:Penaeidae / Q000502:physiology* / Q000821:virology*; D046848:White spot syndrome virus 1 / Q000187:drug effects*; D000438:Alcohols
D007211:Indoles; C005949:tryptophol; D000438:Alcohols
Marsupenaeus japonicus; Shrimp; Thermophile metabolite; Tryptophol; White spot syndrome virus