Eucalyptus oil nanoemulsion-impregnated chitosan film: antibacterial effects against a clinical pathogen, Staphylococcus aureus, in vitro.
Eucalyptus oil (Eucalyptus globulus) nanoemulsion was formulated using low-and high-energy emulsification methods. Development of nanoemulsion was optimized for system parameters such as emulsifier type, emulsifier concentration, and emulsification methods to obtain a lower droplet size with greater stability. The minimized droplet diameter was achieved using the high-energy method of ultrasonication. Tween 80 was more effective in reducing droplet size and emulsion appearance when compared to Tween 20. Stable nanoemulsion was formulated with Tween 80 as a surfactant, and the particle size was found to be 9.4 nm (1:2 v/v). The eucalyptus oil nanoemulsion was impregnated into chitosan (1%) as a biopolymer in varying concentrations. Further, the film was characterized by moisture content, microscopic study, X-ray diffraction, and Fourier transform infrared spectroscopy. Also, the film with and without nanoemulsion was evaluated against Staphylococcus aureus. The nanoemulsion-impregnated chitosan film showed higher antibacterial activity than chitosan film. These results support the inclusion of nanoemulsion-impregnated chitosan film in wound management studies.
Centre for Nanobiotechnology, VIT University, Vellore, India.
Saranya
S
Sugumar
Centre for Nanobiotechnology, VIT University, Vellore, India.
Amitava
A
Mukherjee
Centre for Nanobiotechnology, VIT University, Vellore, India.
Natarajan
N
Chandrasekaran
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000494:pharmacology*; D004655:Emulsions / Q000737:chemistry*; D005052:Eucalyptus / Q000737:chemistry*; D006813:Humidity; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000737:chemistry*; D009822:Oils, Volatile / Q000494:pharmacology*; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects*; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D004655:Emulsions; D009822:Oils, Volatile; D048271:Chitosan
biopolymer; emulsion; essential oil; impregnation; thin film; wound isolate