Effects of salt and waterlogging stresses and their combination on leaf photosynthesis, chloroplast ATP synthesis, and antioxidant capacity in wheat.
The objective of this study was to investigate the effects of salt (ST) and waterlogging (WL) stresses and their combination (SW) on leaf photosynthesis, chloroplast ATP synthesis, and antioxidant capacity in wheat (Triticum aestivum L.). Two winter wheat cultivars, Huaimai 17 and Yangmai 12, differing in their tolerance to ST and WL stresses were used. The plants were grown in pots and were subjected to ST, WL, and SW from 7 days after anthesis (DAA). The WL and SW treatments lasted for 5 days, while the ST treatment was continuously imposed during the grain filling stage. Significant decrease in net photosynthetic rate (PN) of the flag leaf was observed under the ST and SW treatments from 10 DAA in Yangmai 12 and at 18 DAA in both cultivars, which could be stomatal closure related. At 18 DAA, clear reduction in PN under the ST and SW treatments was observed, which was associated with chlorosis, damages to the photosystem II (PSII), enhanced lipid peroxidation, and depressed ATP synthesis in the chloroplasts of the flag leaf. Whereas, WL treatment alone had slightly negative effect on PN, which was mainly attributed to leaf chlorosis and waste in harvested energy by the PSII reaction center dispersed via non-photochemical approaches.
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province 210095, PR China.
Chunfang
C
Zheng
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province 210095, PR China. Electronic address: jiangd@njau.edu.cn.
Dong
D
Jiang
University of Copenhagen, Faculty of Life Sciences, Department of Agriculture and Ecology, Højbakkegaard Allé 13, DK-2630 Taastrup, Denmark.
Fulai
F
Liu
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province 210095, PR China.
Tingbo
T
Dai
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province 210095, PR China.
Qi
Q
Jing
Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, Jiangsu Province 210095, PR China.
Weixing
W
Cao
Ca(2+)-ATPase; Chlorophyll a fluorescence; Lipid peroxidation; Mg(2+)-ATPase; Reactive oxygen species (ROS)