Genome-wide profiling of alternative splicing in Alzheimer's disease.
Alternative splicing is a highly regulated process which generates transcriptome and proteome diversity through the skipping or inclusion of exons within gene loci. Identification of aberrant alternative splicing associated with human diseases has become feasible with the development of new genomic technologies and powerful bioinformatics. We have previously reported genome-wide gene alterations in the neocortex of a well-characterized cohort of Alzheimer's disease (AD) patients and matched elderly controls using a commercial exon microarray platform [1]. Here, we provide detailed description of analyses aimed at identifying differential alternative splicing events associated with AD.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Mitchell K P
MK
Lai
Oxford Project to Investigate Memory and Ageing (OPTIMA), University of Oxford, John Radcliffe Hospital, Oxford, UK.
Margaret M
MM
Esiri
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore ; Department of Clinical Research, Singapore General Hospital, Singapore 169856, Singapore.
Michelle G K
MG
Tan
Alternative splicing; Alzheimer's disease; Exon array; Genome-wide profiling; Transcript variant