[Identification of Pathogens Causing Root Rot Disease on Ligusticum chuanxiong in Sichuan].
OBJECTIVE
To identify the pathogens causing root rot disease on Ligusticum chuanxiong from the main producing areas in Sichuan, and to provide gist of management of this disease in the future.


METHODS
The diseased rhizomes of Ligusticurn chuanxiong with root rot symptom were sampled in two main producing areas in Sichuan. According to Koch's postulate, the pathogenicity on Ligusticum chuanxiong rhizomes of the isolates was confirmed. The representative isolates were identified based on their morphological characteristics and ribosomal DNA internal transcribed spacer(rDNA-ITS) sequences.


RESULTS
The isolates(f2-16, 3-2, f4-19 and f5-7)which caused root rot disease held high homology reached 99%, 99%, 100% and 100% respectively, when compared with GenBank sequences GQ229075, JQ796755, JN232136 and FJ481024.


CONCLUSIONS
The causing agents of root rot disease on Ligusticum chuanxiong belong to Fusarium solani, Fusarium oxysporum, Plectosphaerella cucumerina and Phoma glomerata. Pathogens from different regions are varied.
Jia-sui
JS
Li
Zhu-yun
ZY
Yan
Yin
Y
Lan
Xiao-feng
XF
Shen
Hai
H
Wang
Dong-mei
DM
He
D001203:Ascomycota / Q000145:classification / Q000302:isolation & purification*; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005670:Fusarium / Q000302:isolation & purification*; D030004:Ligusticum / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D027343:Rhizome / Q000382:microbiology
D021903:DNA, Ribosomal Spacer