Occallatibacter riparius gen. nov., sp. nov. and Occallatibacter savannae sp. nov., acidobacteria isolated from Namibian soils, and emended description of the family Acidobacteriaceae.
Three Gram-negative, non-spore-forming, encapsulated bacteria were isolated from a Namibian river-bank soil (strains 277T and 307) and a semiarid savannah soil (strain A2-1cT). 16S rRNA gene sequence analyses placed them within subdivision 1 of the Acidobacteria and revealed 100 % similarity between strains 277T and 307 and 98.2 % similarity between A2-1cT and the former two strains. The closest relatives with validly published names were Telmatobacter bradus, Acidicapsa borealis and Acidicapsa ligni (94.7-95.9 % similarity to the type strains). Cells of all three strains were rod-shaped and motile and divided by binary fission. Ultrastructural analyses revealed a thick cell envelope, resulting mainly from a thick periplasmic space. Colonies of strains 277T and 307 were white to cream and light pink, respectively, while strain A2-1cT displayed a bright pink colour. All three strains were aerobic, chemoheterotrophic mesophiles with a broad temperature range for growth and a moderately acidic pH optimum. Sugars and complex proteinaceous substrates were the preferred carbon and energy sources. A few polysaccharides were degraded. The major quinone in all three strains was MK-8; MK-7 occurred in strain A2-1cT as a minor compound. Major fatty acids were iso-C15 : 0 and iso-C17 : 1ω7c. In addition, iso-C17 : 0 occurred in significant amounts. The DNA G+C contents of strains 277T, 307 and A2-1cT were 59.6, 59.9 and 58.5 mol%, respectively. Based on these characteristics, the three isolates are assigned to two novel species of the novel genus Occallatibacter gen. nov., Occallatibacter riparius sp. nov. [type strain 277T ( = DSM 25168T = LMG 26948T) and reference strain 307 ( = DSM 25169 = LMG 26947)] and Occallatibacter savannae sp. nov. [type strain A2-1cT ( = DSM 25170T = LMG 26946T)]. Together with several other recently described taxa, the novel isolates provide the basis for an emended description of the established family Acidobacteriaceae.
Department of Biology I, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.|Department of Microbial Ecology and Diversity Research, Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
Bärbel U
BU
Foesel
Department of Biology I, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.
Susanne
S
Mayer
Ludwig-Maximilians-Universität München, Department of Biology I, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.
Manja
M
Luckner
Ludwig-Maximilians-Universität München, Department of Biology I, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.
Gerhard
G
Wanner
Central Facility for Microscopy, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Manfred
M
Rohde
Department of Microbial Ecology and Diversity Research, Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.|Department of Biology I, Großhaderner Straße 2-4, D-82152 Martinsried, Germany.|Technische Universität Braunschweig, Braunschweig, Germany.
Jörg
J
Overmann
D061271:Acidobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009276:Namibia; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8