Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex.
Viral terminase enzymes serve as genome packaging motors in many complex double-stranded DNA viruses. The functional motors are multiprotein complexes that translocate viral DNA into a capsid shell, powered by a packaging ATPase, and are among the most powerful molecular motors in nature. Given their essential role in virus development, the structure and function of these biological motors is of considerable interest. Bacteriophage λ-terminase, which serves as a prototypical genome packaging motor, is composed of one large catalytic subunit tightly associated with two DNA recognition subunits. This protomer assembles into a functional higher-order complex that excises a unit length genome from a concatemeric DNA precursor (genome maturation) and concomitantly translocates the duplex into a preformed procapsid shell (genome packaging). While the enzymology of λ-terminase has been well described, the nature of the catalytically competent nucleoprotein intermediates, and the mechanism describing their assembly and activation, is less clear. Here we utilize analytical ultracentrifugation to determine the thermodynamic parameters describing motor assembly and define a minimal thermodynamic linkage model that describes the effects of salt on protomer assembly into a tetrameric complex. Negative stain electron microscopy images reveal a symmetric ring-like complex with a compact stem and four extended arms that exhibit a range of conformational states. Finally, kinetic studies demonstrate that assembly of the ring tetramer is directly linked to activation of the packaging ATPase activity of the motor, thus providing a direct link between structure and function. The implications of these results with respect to the assembly and activation of the functional packaging motor during a productive viral infection are discussed.
Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, Washington.
Teng-Chieh
TC
Yang
Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, Washington.
David
D
Ortiz
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California.
Lyn'Al
L
Nosaka
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California.
Gabriel C
GC
Lander
Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, Washington. Electronic address: carlos.catalano@ucdenver.edu.
Carlos Enrique
CE
Catalano
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001435:Bacteriophages / Q000201:enzymology; D042003:DNA Packaging / Q000502:physiology; D004279:DNA, Viral / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism; D008854:Microscopy, Electron; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D012965:Sodium Chloride / Q000378:metabolism; D013816:Thermodynamics
D004279:DNA, Viral; D012965:Sodium Chloride; D004706:Endodeoxyribonucleases; C030894:terminase; D000251:Adenosine Triphosphatases