Genetic differences in ChTLR15 gene polymorphism and expression involved in Salmonella enterica natural and artificial infection respectively, of Chinese native chicken breeds, with a focus on sexual dimorphism.
Chicken Toll-like receptor 15 (ChTLR15) has been shown to participate in immune activation in response to various pathogens and in the innate defence against infection. Two genetically distinct Chinese breeds of chicken (Qinyuan Partridge and Baier breeds) were used to study the correlation between ChTLR15 single nucleotide polymorphisms and the natural infection status of salmonella in hens, and also to examine genetic and sex-specific effects on ChTLR15 mRNA expression in heterophils and spleen during acute infection with Salmonella enterica serovar Enteritidis (SE) from 1 to 10 days after experimental infection. Three single-nucleotide polymorphisms (G168A, C726T and A1166G) in a single exon of ChTLR15 were identified in the two breeds, but only C726T showed a significant association with salmonella infection. Compared with layer-type Baier chicks, meat-type Qingyuan chicks showed a higher tolerance for capture stress and (SE) infection, as measured, respectively, by the modified body weight of chicks in the control group and in the infection group. Meanwhile, ChTLR15 down-regulation in heterophils and up-regulation in spleen were involved in the response to pathogenic SE colonization during the acute infection period. These significant genetic effects in females led to greater differences in both innate and adaptive immune responses than those exhibited in males. These results suggest that genetics, time and gender play important roles in the modulation of ChTLR15 mRNA level elicited by the SE-mediated immune response differentially in the two genetically distinct breeds, with a focus on sexual dimorphism.
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.|b Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou , Jiangsu 225009 , P.R. China.
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Y
Hu
c Luoyang Pu-like Bio-engineering Co., Ltd , Luoyang , Henan 471000 , P.R. China.
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Chen
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.
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Liu
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.
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YJ
Shan
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.
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Zhu
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.
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Li
a Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding , Institute of Poultry Science of Jiangsu Province , Yangzhou , Jiangsu 225003 , P.R. China.|b Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , Yangzhou , Jiangsu 225009 , P.R. China.
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Zou
D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D015536:Down-Regulation; D005260:Female; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011201:Poultry Diseases / Q000276:immunology* / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology; D019779:Salmonella enterica / Q000502:physiology*; D012727:Sex Characteristics; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D051193:Toll-Like Receptors / Q000235:genetics*; D015854:Up-Regulation; D015430:Weight Gain
D012333:RNA, Messenger; D051193:Toll-Like Receptors
ChTLR15; Chinese native chicken breeds; SNPs; heterophil granulocytes; mRNA expression; salmonella infection; spleen