Influence of Matrix metalloproteinase 1 and 3 genetic variations on susceptibility and severity of juvenile idiopathic arthritis.
Juvenile idiopathic arthritis (JIA) is a chronic rheumatic disease affecting children aged less than 16 years, characterized by chronic synovitis, cartilage damage, and bony erosions mediated by matrix metalloproteinases (MMPs), mainly MMP-1 and MMP-3. The purpose of this study was to investigate MMP-1 and MMP-3 gene polymorphisms in patients with JIA, the role of genes in susceptibility to JIA, and their associations with JIA activity and prognosis. Case-control study included 100 patients diagnosed with JIA, according to the criteria of the International League of Associations for Rheumatology (ILAR), and 100 healthy children, age and sex matched, as controls. The MMP-1 (-1607 1G/2G) and MMP-3 (-1171 5A/6A) polymorphisms were screened by polymerase chain reaction-restriction fragment length polymorphism. The serum levels of MMP-1 and MMP 3 were measured by enzyme-linked immunosorbent assay. There were significant differences between patients with JIA and control groups regarding the genotype and allele frequencies distributions of both MMP-1 1G/2G and MMP-3 5A/6A polymorphisms. The haplotype 2G-6A, which carries the abnormal alleles, showed higher frequencies in patients with JIA than in controls (OD = 2.8, P = 0.002). The prevalence of MMP-1 2G and 6A allele for MMP-3 polymorphism was found to be significantly associated with persistent oligoarticular, rheumatoid factor (RF)-positive polyarthritis, and systemic JIA groups. There were significantly increased serum levels of MMP-1 and MMP-3 associated with 2G/6A haplotype in the patient group, especially with the polyarticular RF (+ve) group than in other groups and the control group. MMP-1 and MMP-3 haplotypes could be useful genetic markers for JIA susceptibility and severity in the juvenile Egyptian population. Moreover, our data further support the use of serum MMP-3 and MMP-1 as specific markers of disease activity in JIA.
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Somia H
SH
Abd-Allah
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Amal S
AS
El-Shal
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Sally M
SM
Shalaby
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Heba F
HF
Pasha
Faculty of Medicine, Rheumatology and Rehabilitation Department, Zagazig University, Zagazig, Egypt.
Amany M
AM
Abou El-Saoud
Faculty of Medicine, Rheumatology and Rehabilitation Department, Zagazig University, Zagazig, Egypt.|Medicine Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
Sahar M
SM
Abdel Galil
Internal Medicine Department, Medical Armed Academy, Cairo, Egypt.
Tysser A
TA
Mahmoud
D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000097:blood / Q000235:genetics*; D019278:Matrix Metalloproteinase 3 / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1
Egyptians; gene polymorphism; juvenile idiopathic arthritis; matrix metalloproteinase-1; matrix metalloproteinase-3