Effects of P-15 Peptide Coated Hydroxyapatite on Tibial Defect Repair In Vivo in Normal and Osteoporotic Rats.
This study assessed the efficacy of anorganic bone mineral coated with P-15 peptide (ABM/P-15) on tibia defect repair longitudinally in both normal and osteoporotic rats in vivo. A paired design was used. 24 Norwegian brown rats were divided into normal and osteoporotic groups. 48 cylindrical defects were created in proximal tibias bilaterally. Defects were filled with ABM/P-15 or left empty. Osteoporotic status was assessed by microarchitectural analysis. Microarchitectural properties of proximal tibial defects were evaluated at 4 time points. 21 days after surgery, tibias were harvested for histology and histomorphometry. Significantly increased bone volume fraction, surface density, and connectivity were seen in all groups at days 14 and 21 compared with day 0. Moreover, the structure type of ABM/P-15 group was changed toward typical plate-like structure. Microarchitectural properties of ABM/P-15 treated newly formed bones at 21 days were similar in normal and osteoporotic rats. Histologically, significant bone formation was seen in all groups. Interestingly, significantly increased bone formation was seen in osteoporotic rats treated with ABM/P-15 indicating optimized healing potential. Empty defects showed lower healing potential in osteoporotic bone. In conclusion, ABM/P-15 accelerated bone regeneration in osteoporotic rats but did not enhance bone regeneration in normal rats.
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, 5000 Odense C, Denmark.
Rasmus
R
Hestehave Pedersen
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, 5000 Odense C, Denmark.
Marina
M
Rasmussen
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, 5000 Odense C, Denmark.
Søren
S
Overgaard
Orthopaedic Research Laboratory, Department of Orthopaedic Surgery and Traumatology, Odense University Hospital, Institute of Clinical Research, University of Southern Denmark, 5000 Odense C, Denmark.
Ming
M
Ding
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry; D002113:Calcification, Physiologic / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000627:therapeutic use*; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D017102:Fracture Healing / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010024:Osteoporosis / Q000628:therapy*; D010446:Peptide Fragments / Q000627:therapeutic use*; D051381:Rats; D013977:Tibia / Q000187:drug effects; D013978:Tibial Fractures / Q000628:therapy*; D055114:X-Ray Microtomography
D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen; D017886:Durapatite