In vivo sub-femtoliter resolution photoacoustic microscopy with higher frame rates.
Microscopy based on non-fluorescent absorption dye staining is widely used in various fields of biomedicine for 400 years. Unlike its fluorescent counterpart, non-fluorescent absorption microscopy lacks proper methodologies to realize its in vivo applications with a sub-femtoliter 3D resolution. Regardless of the most advanced high-resolution photoacoustic microscopy, sub-femtoliter spatial resolution is still unattainable, and the imaging speed is relatively slow. In this paper, based on the two-photon photoacoustic mechanism, we demonstrated a in vivo label free laser-scanning photoacoustic imaging modality featuring high frame rates and sub-femtoliter 3D resolution simultaneously, which stands as a perfect solution to 3D high resolution non-fluorescent absorption microscopy. Furthermore, we first demonstrated in vivo label-free two-photon acoustic microscopy on the observation of non-fluorescent melanin distribution within mouse skin.
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Szu-Yu
SY
Lee
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.|Applied Physics Option, California Institute of Technology, Pasadena, CA 91125, USA.
Yu-Hung
YH
Lai
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Kai-Chih
KC
Huang
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Yu-Hsiang
YH
Cheng
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Tzu-Fang
TF
Tseng
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.|Institute of Physics and Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan.|Molecular Imaging Center and Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.
Chi-Kuang
CK
Sun
D000818:Animals; D008543:Melanins / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D008853:Microscopy / Q000379:methods*; D061088:Photoacoustic Techniques; D012867:Skin / Q000378:metabolism / Q000648:ultrastructure*
D008543:Melanins