Toward understanding the molecular mechanism of a geminivirus C4 protein.
Geminiviruses are ssDNA plant viruses that cause significant agricultural losses worldwide. The viruses do not encode a polymerase protein and must reprogram differentiated host cells to re-enter the S-phase of the cell cycle for the virus to gain access to the host-replication machinery for propagation. To date, 3 Beet curly top virus (BCTV) encoded proteins have been shown to restore DNA replication competency: the replication-initiator protein (Rep), the C2 protein, and the C4 protein. Ectopic expression of the BCTV C4 protein leads to a severe developmental phenotype characterized by extensive hyperplasia. We recently demonstrated that C4 interacts with 7 of the 10 members of the Arabidopsis thaliana SHAGGY-like protein kinase gene family and characterized the interactions of C4 and C4 mutants with AtSKs. Herein, we propose a model of how C4 functions.
a Department of Plant Pathology ; University of Georgia ; Athens , GA USA.
C Michael
CM
Deom
a Department of Plant Pathology ; University of Georgia ; Athens , GA USA.
Katherine
K
Mills-Lujan
D017360:Arabidopsis / Q000201:enzymology; D002462:Cell Membrane / Q000378:metabolism; D017791:Geminiviridae / Q000378:metabolism*; D005810:Multigene Family; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D021381:Protein Transport; D014764:Viral Proteins / Q000378:metabolism*
D014764:Viral Proteins; D011494:Protein Kinases
AtSK; BCTV; C4 protein; cell cycle; curtovirus; geminivirus; hyperplasia; replication