Cell Pluripotency Levels Associated with Imprinted Genes in Human.
Pluripotent stem cells are exhibited similarly in the morphology, gene expression, growth properties, and epigenetic modification with embryonic stem cells (ESCs). However, it is still controversial that the pluripotency of induced pluripotent stem cell (iPSC) is much inferior to ESC, and the differentiation capacity of iPSC and ESC can also be separated by transcriptome and epigenetics. miRNAs, which act in posttranscriptional regulation of gene expression and are involved in many basic cellular processes, may reveal the answer. In this paper, we focused on identifying the hidden relationship between miRNAs and imprinted genes in cell pluripotency. Total miRNA expression patterns in iPSC and ES cells were comprehensively analysed and linked with human imprinted genes, which show a global picture of their potential function in pluripotent level. A new CPA4-KLF14 region which locates in chromosomal homologous segments (CHSs) within mammals and include both imprinted genes and significantly expressed miRNAs was first identified. Molecular network analysis showed genes interacted with imprinted genes closely and enriched in modules such as cancer, cell death and survival, and tumor morphology. This imprinted region may provide a new look for those who are interested in cell pluripotency of hiPSCs and hESCs.
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China.
Liyun
L
Yuan
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China.
Xiaoyan
X
Tang
BasePair BioTechonology Co., Ltd., Shanghai 200235, China.
Binyan
B
Zhang
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, SIBS, CAS, Shanghai 200031, China ; Shanghai Center for Bioinformation Technology, Shanghai 200235, China.
Guohui
G
Ding
D043422:Carboxypeptidases A / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D030561:Databases, Nucleic Acid / Q000706:statistics & numerical data; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D018392:Genomic Imprinting; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008957:Models, Genetic; D015233:Models, Statistical; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051703:Sp Transcription Factors / Q000235:genetics
C417549:KLF14 protein, human; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs; D051703:Sp Transcription Factors; C539656:CPA4, human; D043422:Carboxypeptidases A