Presence of cytomegalovirus in the perilymphatic fluid of patients with profound sensorineural hearing loss caused by congenital cytomegalovirus infection.
CONCLUSIONS
Not all patients diagnosed with congenital infection using umbilical cord assay were found to be positive for CMV-DNA by perilymphatic fluid assay. In addition, a CMV-DNA-positive result was observed in one patient who had not been diagnosed with congenital infection. Sampling of perilymphatic fluid from a large population of patients with congenital SNHL caused by congenital CMV infection or of unknown etiology is required to determine the prevalence of CMV-related profound HL.


OBJECTIVE
Sensorineural hearing loss (SNHL) is one of the most frequent manifestations in patients with congenital cytomegalovirus (CMV) infection. Using dried umbilical cord, a PCR-based assay was recently developed for the retrospective detection of congenital CMV infection. This study analyzed the presence of CMV in the perilymphatic fluid and evaluated differences in the effect of cochlear implantation between CMV-positive and -negative groups.


METHODS
Perilymphatic fluid was collected from each patient at the time of cochlear implantation and analyzed for the presence of CMV using a PCR method.


RESULTS
The perilymphatic fluid in two of the five patients suffering from congenital CMV infection and in one of the 17 patients without congenital CMV infection was found to be positive for CMV.
a Department of Otorhinolaryngology , Fukushima Medical University Aizu Medical Center , Aizuwakamatsu , Japan.
Hiroshi
H
Ogawa
b Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.
Takamichi
T
Matsui
b Department of Otolaryngology , Fukushima Medical University , Fukushima , Japan.
Yoko
Y
Baba
c Fukushima Rehabilitation Center for Children , Fukushima , Japan.
Naoko
N
Yamada
c Fukushima Rehabilitation Center for Children , Fukushima , Japan.
Yukie
Y
Suzuki
d Department of Microbiology , Fukushima Medical University , Fukushima , Japan.
Tatsuo
T
Suzutani
D002675:Child, Preschool; D019929:Cochlear Implantation; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000151:congenital / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D006319:Hearing Loss, Sensorineural / Q000175:diagnosis / Q000209:etiology* / Q000601:surgery; D006801:Humans; D008297:Male; D010498:Perilymph / Q000821:virology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies
D004279:DNA, Viral
Dried umbilical cord; PCR; cochlear implantation; inner ear; viral infection