Development and evaluation of sunscreen creams containing morin-encapsulated nanoparticles for enhanced UV radiation protection and antioxidant activity.
The objective of present work was to develop novel sunscreen creams containing polymeric nanoparticles (NPs) of morin. Polymeric NPs containing morin were prepared and optimized. The creams containing morin NPs were also prepared and evaluated. Optimized NPs exhibited particle size of 90.6 nm and zeta potential of -31 mV. The entrapment efficiency of morin, within the polymeric NPs, was found to be low (12.27%). Fourier transformed infrared spectroscopy and differential scanning calorimetry studies revealed no interaction between morin and excipients. Transmission electron microscopy and atomic force microscopy revealed that the NPs were spherical in shape with approximately 100 nm diameter. Optimized NPs showed excellent in vitro free radical scavenging activity. Skin permeation and deposition of morin from its NPs was higher than its plain form. Different sunscreen creams (SC1-SC8) were formulated by incorporating morin NPs along with nano zinc oxide and nano titanium dioxide. SC5 and SC8 creams showed excellent sun protection factor values (≈40). In vitro and in vivo skin permeation studies of sunscreen creams containing morin NPs indicated excellent deposition of morin within the skin. Morin NPs and optimized cream formulations (SC5 and SC8) did not exhibit cytotoxicity in Vero and HaCaT cells. Optimized sunscreen creams showed excellent dermal safety. SC5 and SC8 creams demonstrated exceptional in vivo antioxidant effect (estimation of catalase, superoxide dismutase, and glutathione) in UV radiation-exposed rats. The optimized sunscreen creams confirmed outstanding UV radiation protection as well as antioxidant properties.
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Pallavi Krishna
PK
Shetty
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Venkatesh
V
Venuvanka
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Hitesh Vitthal
HV
Jagani
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Gejjalagere Honnappa
GH
Chethan
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Virendra S
VS
Ligade
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Prashant B
PB
Musmade
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Usha Y
UY
Nayak
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Meka Sreenivasa
MS
Reddy
Division of Clinical Embryology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India.
Guruprasad
G
Kalthur
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Nayanabhirama
N
Udupa
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Chamallamudi Mallikarjuna
CM
Rao
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India.
Srinivas
S
Mutalik
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002214:Capsules; D002460:Cell Line; D002626:Chemistry, Pharmaceutical; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010539:Permeability; D011108:Polymers / Q000737:chemistry; D051381:Rats; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013473:Sunscreening Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry; D014466:Ultraviolet Rays / Q000009:adverse effects*; D015034:Zinc Oxide / Q000737:chemistry; D047309:Flavones
D000975:Antioxidants; D002214:Capsules; D005419:Flavonoids; D011108:Polymers; D013473:Sunscreening Agents; D014025:Titanium; D015034:Zinc Oxide; C009495:titanium dioxide; C008548:morin; D047309:Flavones
morin; nanoparticles; skin permeation; sun protection factor; sunscreen