Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA.
A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.
Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
Teegala Lakshminarayan
TL
Reddy
Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
P Sivarama
PS
Krishnarao
Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
Garikapati Koteswara
GK
Rao
Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
Eswar
E
Bhimireddy
Department of Chemistry, Sri Venkateswara University, Tirpupati, India.
P
P
Venkateswarlu
Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
Debendra K
DK
Mohapatra
Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India.
J S
JS
Yadav
Functional Genomics and Gene Silencing Group, CSIR-Indian Institute of Chemical Technology, CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.
Utpal
U
Bhadra
Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.
Manika Pal
MP
Bhadra
D017209:Apoptosis; D001565:Benzoates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D016503:Drug Delivery Systems; D004705:Endocytosis; D005434:Flow Cytometry; D020868:Gene Silencing; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D001565:Benzoates; D001672:Biocompatible Materials; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D034741:RNA, Small Interfering
biocompatibility; cell uptake; endocytosis; gene silencing; nanoparticles; siRNA