Translation Analysis at the Genome Scale by Ribosome Profiling.
Ribosome profiling is an emerging approach using deep sequencing of the mRNA part protected by the ribosome to study protein synthesis at the genome scale. This approach provides new insights into gene regulation at the translational level. In this review we describe the protocol to prepare polysomes and extract ribosome protected fragments before to deep sequence them.
Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA, CNRS, Université Paris Sud, Bâtiment 400, 91405, Orsay, France.
Agnès
A
Baudin-Baillieu
Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA, CNRS, Université Paris Sud, Bâtiment 400, 91405, Orsay, France.
Isabelle
I
Hatin
Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA, CNRS, Université Paris Sud, Bâtiment 400, 91405, Orsay, France.
Rachel
R
Legendre
Institute for Integrative Biology of the Cell (I2BC), UMR 9198 CEA, CNRS, Université Paris Sud, Bâtiment 400, 91405, Orsay, France. olivier.namy@igmors.u-psud.fr.
Olivier
O
Namy
D016678:Genome; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D011132:Polyribosomes / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012270:Ribosomes / Q000235:genetics*
D012333:RNA, Messenger
Recoding; Ribo-seq; Ribosome profiling; Translation regulation