Differences in urinary proteins related to surgical  margin status after radical prostatectomy.
Presented exploratory pilot study was aimed at evaluation of proteins present in urinary specimens collected from prostate cancer suffering subjects after radical prostatectomy, divided into two experimental cohorts: positive (n=15) and negative (n=15) surgical margins (PSM/NSM). The presence of PSM suggests inadequate cancer clearance and the possible need for additional treatment. Proper identification of these risk-patients is therefore of a paramount importance. Total protein profiles were firstly identified by using SDS-PAGE and compared by using partial least square discrimination analysis (PLS-DA), which revealed differences in molecular weights of 80-99 and 150-235 kDa between the experimental groups. For further identification of proteins, comparative proteomic technologies were employed. Two-dimensional gel electrophoresis with subsequent identification of protein spots by using MALDI-TOF mass fingerprinting revealed differential expression of proteins between NSM/PSM cohorts. Moreover, in PSM group, three uniquely identified proteins (cyclin-dependent kinase 6, galectin-3-binding protein and L-lactate dehydrogenase C chain) were found, which show tight connection with prostate cancer and presence of all of them was previously linked to certain aspects of prostate cancer. These proteins may be associated with the molecular mechanisms of prostate cancer development; hence, their identification may be helpful for the assessment of disease progression risk after radical prostatectomy, but also for possible early diagnosis.
Zbynek
Z
Heger
Petr
P
Michalek
Roman
R
Guran
Natalia
N
Cernei
Katerina
K
Duskova
Stepan
S
Vesely
Jiri
J
Anyz
Olga
O
Stepankova
Ondrej
O
Zitka
Vojtech
V
Adam
Rene
R
Kizek
D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000652:urine; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000652:urine; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000652:urine; D051361:Cyclin-Dependent Kinase 6 / Q000096:biosynthesis* / Q000652:urine; D018572:Disease-Free Survival; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000096:biosynthesis* / Q000652:urine; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000652:urine; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis* / Q000652:urine; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery* / Q000652:urine; D040901:Proteomics; D016326:Extracellular Matrix Proteins
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D051361:Cyclin-Dependent Kinase 6; D006023:Glycoproteins; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins; C409673:lactate dehydrogenase C4; C495911:CDK6 protein, human