siRNA Therapy, Challenges and Underlying Perspectives of Dendrimer as Delivery Vector.
siRNA technology presents a helpful means of gene silencing in mammalian cells. Advancement in the field includes enhanced attentiveness in the characterization of target and off-target effects employing suitable controls and gene expression microarrays. These will permit expansion in the measurement of single and multiple target combinations and also permit comprehensive efforts to understand mammalian cell processes. Another fact is that the delivery of siRNA requires the creation of a nanoparticulate vector with controlled structural geometry and surface modalities inside the targeted cells. On the other hand, dendrimers represent the class of carrier system where massive control over size, shape and physicochemical properties makes this delivery vector exceptional and favorable in genetic transfection applications. The siRNA therapeutics may be incorporated inside the geometry of the density controlled dendrimers with the option of engineering the structure to the specific needs of the genetic material and its indication. The existing reports on the siRNA carrying and deliverance potential of dendrimers clearly suggest the significance of this novel class of polymeric architecture and certainly elevate the futuristic use of this highly branched vector as genetic material delivery system.
School of Pharmacy, Department of Pharmaceutical Technology, International Medical University, JalanJalil Perkasa 19, 57000 Kuala Lumpur, Malaysia. rakeshtekade@gmail.com.
Rakesh Kumar
RK
Tekade
Rahul G S
RG
Maheshwari
Piyoosh A
PA
Sharma
Muktika
M
Tekade
Abhay Singh
AS
Chauhan
D000818:Animals; D050091:Dendrimers / Q000737:chemistry*; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D053758:Nanoparticles; D011108:Polymers / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage*
D050091:Dendrimers; D011108:Polymers; D034741:RNA, Small Interfering