[Fingerprint and Simultaneous Determination of Multi-components in Water-soluble Components of Salvia miltiorrhiza in Miao Autonomous County of Songtao, Guizhou].
OBJECTIVE
To establish the HPLC fingerprint of water-soluble components of Salvia miltiorrhiza in Songtao, Guizhou, and to perform simultaneous determination of six components in it, so as to provide analytical method for its quality control.


METHODS
The analyses were performed on a Phenomenex Luna C18 (2) (250 mm x 4. 6 mm, 5µm) column eluted with 0. 4% formic acid(A) - acetonitrile(B) in a gradient mode. The flow rate was 1. 0 mL/min, column temperature was set at 30 °C.


RESULTS
Eleven common peaks were identified form the HPLC fingerprint of Salvia miltiorrhiza from 10 batches, the HPLC fingerprint similarities of 10 batches were not less than 0. 999. The linear ranges of danshensu, protocatechuic aldehyde, caffeic acid, rosmarinic acid, lithospermic acid and salvianolic acid B were 0. 0680 ~ 1. 3583 mg/mL, 0. 0008 ~ 0. 3967 mg/mL, 0. 0005 ~ 0. 2660 mg/mL, 0. 0020 ~ 0. 3992 mg/mL, 0. 0063 ~ 0. 6311 mg/mL and 0. 0097 ~ 1. 9306 mg/mL with r ≥ 0. 9999, respectively. The recovery rates were 100. 84%,102. 44%, 100. 53% ,100. 63%, 100. 83% and 100. 35% with RSD <2. 3%, respectively.


CONCLUSIONS
The established method is simple, accurate and can provide reference for quality control of Salvia miltiorrhiza.
Tian-ling
TL
Chen
Chang-qiong
CQ
Bi
Xue
X
Xiao
Xian-peng
XP
Ma
Fa-huan
FH
Ge
D001547:Benzaldehydes; D001572:Benzofurans; D002109:Caffeic Acids; D002396:Catechols; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D007773:Lactates; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D011786:Quality Control; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014867:Water; D000097123:Rosmarinic Acid
D001547:Benzaldehydes; D001572:Benzofurans; D002109:Caffeic Acids; D002396:Catechols; D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D007773:Lactates; D064209:Phytochemicals; D014867:Water; D000097123:Rosmarinic Acid; C046833:lithospermic acid; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde; C076944:salvianolic acid B; C040048:caffeic acid