[Therapeutic Effect of Paconol on Alcoholic Fatty Liver in Rats].
OBJECTIVE
To make clear the therapeutic effect of paconol on alcoholic fatty liver disease (AFLD).


METHODS
Model rats of AFLD were established with alcohol intragastric administration. Paconol was applied to treat the model rats for four weeks(75, 150 and 300 mg/kg), with silybin as control administration. The content of TC and TG in serum and liver were determined with 4-Aminoantipyrine (4-AAP) method, ALT and AST levels were determined with Reitman-Frankel method, serum HDL content with direct method, serum LDL content with precipitation method, serum TNF-α content with enzyme linked immunosorbent assay sandwich technique, FFA content in serum and liver with enzymic colorimetric method, MDA content with thiobarbituric acid reactive substance assay method, liver CYP2E1 expression with SABC method, and the pathological changes of liver were observed directly or with optical microscope.


RESULTS
Paconol lowered TC, TG, HDL, LDL, ALT, AST, TNF-α, FFA and MDA levels in serum, as well as TC, TG and FFA levels in liver, inhibited the expression of protein CYP2E1, and improved the pathological changes of model rats.


CONCLUSIONS
There is a certain therapeutic effect of paconol on AFLD in rats.
Gui-ming
GM
Yan
Min
M
Dai
Zi-hua
ZH
Xuan
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005235:Fatty Liver, Alcoholic / Q000188:drug therapy*; D008099:Liver / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D004365:Drugs, Chinese Herbal; D014409:Tumor Necrosis Factor-alpha