An overview of the use of SNaPshot for predicting blood group antigens.
The use of SNaPshot (Applied Biosystems, Foster City, CA) for predicting blood group antigens has emerged as an alternative to hemagglutination testing and also to the current low- and highthroughput blood group genotyping methods. Several groups have developed multiplex-polymerase chain reaction SNaPshot assays to determine single nucleotide polymorphisms (SNPs) in blood group genes with the purpose of identifying clinically relevant antigens and rare alleles. The selection of SNPs is based on the population or laboratory reality and the purpose of the genatyping. Unlike high-throughput genotyping strategies that are provided as commercial platforms, the SNPs can be chosen to best meet the needs of the user, and the interpretation of the results do not depend on the manufacturer.
PhD, Director, Technical and Scientific Department, Colsan - Associação Beneficente de Coleta de Sangue, Avenida Jandira, 1260 - Indianópolis - 04080-006, São Paulo, SP, Brazil.
F R M
FR
Latini
PhD, Professor and Researcher (corresponding author), Hemocentro - Unicamp, Rua Carlos Chagas, 480 - Barão Geraldo - 13083-878, Campinas, SP, Brazil.
Lilian M
LM
Castilho
D000483:Alleles; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
D001789:Blood Group Antigens