Negatively Charged Carbon Nanohorn Supported Cationic Liposome Nanoparticles: A Novel Delivery Vehicle for Anti-Nicotine Vaccine.
Tobacco addiction is the second-leading cause of death in the world. Due to the nature of nicotine (a small molecule), finding ways to combat nicotine's deleterious effects has been a constant challenge to the society and the medical field. In the present work, a novel anti-nicotine vaccine based on nanohorn supported liposome nanoparticles (NsL NPs) was developed. The nano-vaccine was constructed by using negatively charged carbon nanohorns as a scaffold for the assembly of cationic liposomes, which allow the conjugation of hapten conjugated carrier proteins. The assembled bio-nanoparticles are stable. Mice were immunized subcutaneously with the nano-vaccine, which induced high titer and high affinity of nicotine specific antibodies in mice. Furthermore, no evidence of clinical signs or systemic toxicity followed multiple administrations of NsL-based anti-nicotine vaccine. These results suggest that NsL-based anti-nicotine vaccine is a promising candidate in treating nicotine dependence and could have potential to significantly contribute to smoking cessation.
Hong
H
Zheng
Yun
Y
Hu
Wei
W
Huang
Sabina
S
de Villiers
Paul
P
Pentel
Jianfei
J
Zhang
Harry
H
Dorn
Marion
M
Ehrich
Chenming
C
Zhang
D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D001835:Body Weight / Q000276:immunology; D002244:Carbon / Q000737:chemistry*; D004326:Drinking / Q000276:immunology; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009538:Nicotine / Q000737:chemistry / Q000276:immunology*; D010316:Particle Size; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014029:Tobacco Use Disorder / Q000517:prevention & control; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology*
D007074:Immunoglobulin G; D008081:Liposomes; D014612:Vaccines; D009538:Nicotine; D002244:Carbon