Induction and identification of Stylosanthes guianensis tetraploids.
Stylosanthes guianensis is an elite and important forage legume species, which is extensively cultivated in tropical areas. Polyploid breeding via exposure to colchicine is a conventional and practical method to improve varieties of S. guianensis. Terminal buds of S. guianensis Reyan No.5 seedlings were treated with different concentrations of colchicine (0.00, 0.05, 0.10, 0.15, 0.20, and 0.25%) for 24, 48, and 72 h. Morphological and cytological variants were observed at a frequency of <96% among transplanted seedlings. The cytogenetic analysis of young leaf cells was conducted on all variants to identify their ploidy levels. The most efficient procedure for tetraploid production was the treatment of seedling apical buds with 20% colchicine for 48 h, with the tetraploid induction rate being 10%. This is a relatively simple and reliable method for the production of tetraploidy in S. guianensis.
College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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FH
Wu
College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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XD
Yu
College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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NS
Zhuang
Institute of Tropical Crops Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan Province, China.
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GD
Liu
College of Agronomy, Hainan University, Haikou, Hainan Province, China.
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JP
Liu
D032461:Chromosomes, Plant / Q000187:drug effects / Q000235:genetics; D003078:Colchicine / Q000494:pharmacology; D007887:Fabaceae / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D057891:Tetraploidy
D003078:Colchicine