Potential of serum metabolites for diagnosing post-stroke cognitive impairment.
Cognitive impairment commonly accompanies clinical syndromes associated with stroke. The identification of laboratory markers of post-stroke cognitive impairment (PSCI) may help detect patients at increased risk of cognitive deterioration and determine the appropriate treatment regimes. A non-targeted metabolomics approach based on ultra-high performance liquid chromatography coupled with Q-TOF mass spectrometry was applied to study PSCI. The stroke patients were significantly distinguishable from the healthy subjects. Stroke patients could be well-stratified based on cognitive impairment. Several differential serum metabolites were further identified for post-stroke non-cognitive impairment (PSNCI) and PSCI patients, suggesting metabolic dysfunction in inflammation, neurotoxicity, bioenergetic homeostasis, oxidative stress, and apoptosis. In total, three serum metabolites (glutamine, kynurenine, and LysoPC(18:2)) were identified as candidate diagnostic biomarkers for PSCI, and their combined use yielded good diagnostic capacity for PSCI by receiver operating characteristic curves. The present metabolomics study provided a novel strategy for stratifying stroke patients with cognitive impairment using serum-based metabolite markers, which could be of great importance in understanding the pathological mechanisms and determining the appropriate treatment regimes of PSCI patients.
Departments of Physiology, College of Basic Medicine, Second Military Medical University, Shanghai 200433, China. wangwz68@hotmail.com.
Min
M
Liu
Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. bixy616@163.com.
Kaige
K
Zhou
Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. bixy616@163.com.
Hailong
H
Li
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Xin
X
Dong
Department of Pharmaceutical Analysis, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
Guangguo
G
Tan
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Yifeng
Y
Chai
Departments of Physiology, College of Basic Medicine, Second Military Medical University, Shanghai 200433, China. wangwz68@hotmail.com.
Weizhong
W
Wang
Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. bixy616@163.com.
Xiaoying
X
Bi
D015415:Biomarkers; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003072:Cognition Disorders / Q000097:blood* / Q000175:diagnosis / Q000209:etiology*; D005260:Female; D006801:Humans; D008297:Male; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D012372:ROC Curve; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020521:Stroke / Q000150:complications*
D015415:Biomarkers