Mitogen-activated protein kinase kinase kinase (MAPKKK) 4 from rapeseed (Brassica napus L.) is a novel member inducing ROS accumulation and cell death.
MAPKKK is the largest family of MAPK cascade, which is known to play important roles in plant growth, development and immune responses. So far, only a few have been functionally characterized even in the model plant, Arabidopsis due to the potential functional redundancy of MAPKKK. We previously identified and cloned a few MAPKKK family genes from rapeseed. In this study, BnaMAPKKK4 was characterized as a member in eliciting accumulation of reactive oxygen species (ROS) and hypersensitive response (HR)-like cell death. This is accompanied with accumulation of malondialdehyde (MDA), anthocyanin as well as nuclear DNA fragmentation. The transcript abundance of a series of ROS accumulation, cell death, and defense response related genes were up-regulated by the expression of MAPKKK4. Further investigation identified BnaMAPKKK4 elicited ROS through the downstream MPK3. These results indicate that BnaMAPKKK4 and its downstream components function in the ROS-induced cell death.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 18710470987@163.com.
Liang
L
Li
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: yechaofei001@163.com.
Chaofei
C
Ye
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 571828628@qq.com.
Rui
R
Zhao
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 1458272138@qq.com.
Xin
X
Li
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: happywuzhenliu@163.com.
Wu-zhen
WZ
Liu
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: 283915941@qq.com.
Feifei
F
Wu
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: yanjingli512@163.com.
Jingli
J
Yan
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: jiangyq@nwafu.edu.cn.
Yuan-Qing
YQ
Jiang
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, 712100, China. Electronic address: yangwl@nwafu.edu.cn.
Bo
B
Yang
D029688:Brassica napus / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D048768:MAP Kinase Kinase Kinase 4 / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D059828:Plant Cells / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics
D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D048768:MAP Kinase Kinase Kinase 4
Cell death; MAPKKK4; MPK; ROS; Rapeseed