Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects.
The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. The comet assay (CA) was used to evaluate the clastogenic potential of BPV. The results confirmed the presence of BPV-2, 3, 5, and 9 in infected samples. Histopathological analysis revealed acanthosis, koilocytosis, hypergranulosis, hyperkeratosis, and transformed fibroblasts.E7 and L1 BPV proteins were detected in the epithelium, as well as in the connective tissues, indicating productive infection at different sites. CA results showed that BPV-2, 5, and 9 exhibit the same level of clastogenicity. These findings support the oncogenic action of BPV in establishing a favorable microenvironment for oncogenesis.
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R P
RP
Araldi
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
T C
TC
Melo
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
A C
AC
Neves
Laboratório de Fisiopatologia, Instituto Butantan, São Paulo, SP, Brasil.
D D
DD
Spadacci-Morena
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R F
RF
Magnelli
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
D G
DG
Modolo
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
P L
PL
de-Sá-Júnior
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
J
J
Mazucchelli-de-Souza
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R F
RF
Carvalho
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
W
W
Beçak
Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brasil.
R C
RC
Stocco
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000145:classification / Q000235:genetics; D063646:Carcinogenesis; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology* / Q000821:virology*; D020552:Comet Assay; D004279:DNA, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral