Preparation of Silicon-Carbon-Based Dots@Dopamine and Its Application in Intracellular Ag(+) Detection and Cell Imaging.
A novel nanocomposite, silicon-carbon-based dots@dopamine (Si-CDs@DA) was prepared using (3-aminopropyl) triethoxysilane, glycerol, and dopamine as raw materials via a rapid microwave-assisted irradiation. This type of Si-CDs@DA exhibited ultrabright fluorescence emission (quantum yield of 12.4%) and could response to Ag(+) selectively and sensitively. Moreover, the obtained Si-CDs@DA can be further applied in sensing intracellular Ag(+) and cell imaging, because of its photostability, salt stability, and low cytotoxicity. This study provides a simple and efficient approach for preparing novel Ag(+) fluorescent probes, which could expand the application of carbon nanomaterials in designing related biosensors.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing 210023, P. R. China.
Yuliang
Y
Jiang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing 210023, P. R. China.
Zhaoyin
Z
Wang
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University , Nanjing 210023, P. R. China.
Zhihui
Z
Dai
D002244:Carbon / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D012834:Silver / Q000378:metabolism*
D012834:Silver; D002244:Carbon; D012825:Silicon
Si-CDs@DA; carbon-based dots; cell imaging; fluorescent probe; intracellular Ag+ sensing