A novel Bunyavirus from the soft tick, Argas vespertilionis, in Japan.
Soft ticks, Argas vespertilionis, were collected from feces of bats in Japan. Cytopathic effect (CPE) was observed after inoculating the homogenates of ticks to Vero cells. Sequencing of RNA extracted from the cell supernatant was performed by next generation sequencer. The contigs had identity to segments of Bunyaviruses, Issyk-Kul virus. The identities of segment L, M and S were only 77, 76 and 79% to Issyk-Kul virus, respectively. Therefore, we named this novel virus Soft tick bunyavirus (STBV). In the phylogenetic tree, segment L of STBV was closely related to a cluster consisting of the genus Nairovirus of the family Bunyaviridae.
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Mami
M
Oba
Tsutomu
T
Omatsu
Ai
A
Takano
Hiromi
H
Fujita
Kozue
K
Sato
Atsushi
A
Nakamoto
Mamoru
M
Takahashi
Nobuhiro
N
Takada
Hiroki
H
Kawabata
Shuji
S
Ando
Tetsuya
T
Mizutani
D000818:Animals; D026864:Argas / Q000821:virology*; D002685:Chiroptera / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D059014:High-Throughput Nucleotide Sequencing; D007564:Japan; D016851:Orthobunyavirus / Q000145:classification / Q000302:isolation & purification*; D010802:Phylogeny