Interaction Between Breast Cancer Cells and Adipose Tissue Cells Derived from Fat Grafting.
BACKGROUND
Adipose tissue transplantation has the benefit of providing both regenerative and aesthetic outcomes in breast cancer treatment. However, the transplanted tissue can stimulate the growth of residual cancer cells.


OBJECTIVE
The aim of this study is to identify the interactions between adipose tissue cell subpopulations and human cancer cell lines.


METHODS
Intact adipose tissue from lipofilling procedures as well as fibroblasts derived from adipose tissue, were cocultured in the presence of MDA-MB-231, MCF-7 e ZR-75-1 breast cancer cell lines. The influence on cancer cell lines of fibroblasts, induced to differentiate into specific adipocytes, was also assayed.


RESULTS
All cancer cell lines displayed a significant increase in proliferation rate when cocultured in the presence of either intact adipose tissue or induced adipocytes. To a lesser extent, uninduced fibroblasts stimulate breast cancer cell proliferation.


CONCLUSIONS
Recent studies have shown that the microenvironment surrounding breast cancer cells may stimulate growth and promote progression of residual cancer cells when surgery is performed on the main tumor mass. Accordingly, the graft of adipose tissue could potentially promote or accelerate the development of a subclinical tumor or support its locoregional recurrence. Our data suggest that adipocytes have a remarkable influence on the proliferation of cancer cell lines. The oncological safety of the lipofilling procedure outcome is still debated; thus, further studies and consistent follow-up examination are needed.
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Michela
M
Massa
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Simona
S
Gasparini
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Ilaria
I
Baldelli
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Linda
L
Scarabelli
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Pierluigi
P
Santi
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Rodolfo
R
Quarto
Dr Massa is a Plastic Surgeon and Fellow, Dr Baldelli is a Plastic Surgeon and Assistant Professor, and Dr Santi is a Plastic Surgeon and Full Professor, Department of Integrated Surgical and Diagnostic Sciences, University of Genoa, IRCCS San Martino IST, Genoa, Italy. Ms Gasparini is a Researcher and Fellow, Ms Scarabelli is a Researcher, Dr Quarto is a Researcher and Full Professor, and Dr Repaci is a Researcher and Fellow, Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Erica
E
Repaci
D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation; D000328:Adult; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002450:Cell Communication; D049109:Cell Proliferation; D054338:Cell Transdifferentiation; D018920:Coculture Techniques; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D061986:MCF-7 Cells; D008875:Middle Aged; D010641:Phenotype