Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis.
BACKGROUND
Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling, including FOXO3a, which encodes a transcription factor known to promote inflammation in several tissues. However, FOXO3a expression in tissues affected by UC has not been examined. This study investigated the effects of FOXO3a on UC pathogenesis.


METHODS
FOXO3a expression, in 23 patients with UC and in HT29 cells treated with tumor necrosis factor-α (TNF-α) for various durations, was detected by quantitative real-time polymerase chain reaction and Western blotting analysis. Enzyme-linked immunosorbent assay was used to quantify interleukin (IL)-8 expression in FOXO3a-silenced HT29 cells treated with TNF-α for various durations.


RESULTS
The messenger RNA and protein expression of FOXO3a were significantly lower in UC tissues than those in normal subjects (P < 0.01). TNF-α treatment for 0, 0.5, 1, 6, and 24 h induced FOXO3 degradation in HT29 cells. FOXO3a silencing increased IL-8 levels in HT29 cells treated with TNF-α for 6 h (P < 0.05).


CONCLUSIONS
FOXO3a may play an important role in the intestinal inflammation of patients with UC.
Min
M
Min
Jing
J
Yang
Department of Gastroenterology and Hepatology, Institute of Digestive Diseases, Chinese People's Liberation Army General Hospital, Beijing 100853, China.
Yun-Sheng
YS
Yang
Yan
Y
Liu
Li-Mei
LM
Liu
Yang
Y
Xu
D000328:Adult; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000071316:Forkhead Box Protein O3 / Q000235:genetics / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C508606:CXCL8 protein, human; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha