Upregulation of MALAT-1 and its association with survival rate and the effect on cell cycle and migration in patients with esophageal squamous cell carcinoma.
The aim of this study is to investigate whether metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) can be used as a potential therapy target for human esophageal squamous cell carcinoma. MALAT-1 expression levels were detected in 137 paired EC samples and adjacent nonneoplastic tissues. Human esophageal carcinoma cell lines EC9706 and KYSE150 were transfected with MALAT-1 small interference RNA. Cell proliferation, migration/invasion ability, cell cycle, and apoptosis were assessed. MALAT-1 expressed higher levels in esophageal cancer tissues when compared with paired adjacent normal tissues. This high expression was associated with a decreased survival rate. MALAT-1 knockdown induced a decrease in proliferation-enhanced apoptosis, inhibited migration/invasion, and reduced colony formation and led to cell cycle arrest at the G2/M phase. These data indicates that MALAT-1 could be exploited for therapeutic benefit.
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Wenjian
W
Yao
Department of Pathology, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.
Yu
Y
Bai
Department of Nosocomial Infection Administration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Yan
Y
Li
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Ling
L
Guo
Department of Nosocomial Infection Administration, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Ping
P
Zeng
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Yonglian
Y
Wang
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Bo
B
Qi
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Shangguo
S
Liu
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China.
Xiuguang
X
Qin
Department of Pathology, Xinxiang Medical University, Xinxiang, Henan, 453003, People's Republic of China.
Yongzhen
Y
Li
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, People's Republic of China. drbszhao@163.com.
Baosheng
B
Zhao
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics
C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
Esophageal carcinoma; Long noncoding RNA; Metastasis-associated lung adenocarcinoma transcript 1; Small RNA interference