PKM2 promotes stemness of breast cancer cell by through Wnt/β-catenin pathway.
Metastasis is one of the main causes of breast cancer (BCa)-related deaths in female. It has been reported that cancer stem cell played an important role in metastasis. Here we first revealed a specific role of pyruvate kinase isozymes M2 (PKM2) in the stemness of breast cancer cells. Breast cancer tissue analysis confirmed the upregulation of PKM2 in breast cancer, and high PKM2 levels were associated with poor prognosis of breast cancer patients. Holoclone assay and colony formation assay significantly elucidated the role of PKM2 in the self-renewal of breast cancer cells. Moreover, PKM2 elevated the proportion of stem cell and the ability of sphere formation in breast cancer cells. PKM2 played its functional role in stemness by regulating β-catenin. Collectively, we identified critical roles of PKM2 in the stemness of breast cancer cells which may elevate the therapeutic effect on breast cancer patients.
Department of Oncology, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China.|Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Zheng
Z
Zhao
Breast Cancer Program, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Zhangjun
Z
Song
Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Zijun
Z
Liao
Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Zhigang
Z
Liu
Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Haifeng
H
Sun
Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Baoxia
B
Lei
Third Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, 710061, People's Republic of China.
Wenjuan
W
Chen
Department of Oncology, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China. dcxcancer@163.com.
Chengxue
C
Dang
D001943:Breast Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D000066673:Cell Self Renewal; D005260:Female; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000502:physiology; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000201:enzymology*; D011379:Prognosis; D011770:Pyruvate Kinase / Q000502:physiology*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
C495270:CTNNB1 protein, human; D007527:Isoenzymes; D051176:beta Catenin; D011770:Pyruvate Kinase
Cancer stem cell; Holoclone; Pyruvate kinase isozymes M2; Stemness; β-Catenin