cFLIP expression is altered in severe corticosteroid-resistant asthma.
Dysregulation of alternative splicing of mRNA precursors is known to contribute to numerous human diseases. In this study we carried out the first systematic search for asthma-associated changes in alternative splicing events, using a model of Aspergillus fumigatus (A. fumigatus)-sensitized mice and an exon junction microarray to detect potential changes in alternative splicing. One of the sensitization-associated changes identified in the search was a shift in alternative splicing of the mRNA encoding cFLIP, a modulator of the caspase-mediated extrinsic apoptosis pathway. Expanding these studies to human asthma patients, we discovered a significant decrease in the expression of both cFLIP isoforms in severe corticosteroid-resistant asthmatics. Although it is unclear whether these changes were due solely to differences in alternative splicing, these findings provide evidence that dysregulation of the extrinsic apoptosis pathway is part of the underlying immunopathogenesis of severe refractory asthma.
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Dennis
D
Lazarev
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA ; Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Rachel L
RL
Miller
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Columbia University, New York, NY 10032, USA.
Emily
E
Dimango
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA.
Xian-Dong
XD
Fu
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0651, USA.
Hai-Ri
HR
Li
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Charlotte J
CJ
Logan
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
James L
JL
Manley
Alternative splicing; Asthma; Microarrays