Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry.
Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.
Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 64, Sion 2, CH-1950, Switzerland.
Denis
D
Prim
Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 64, Sion 2, CH-1950, Switzerland. marc.pfeifer@hevs.ch.
Marc E
ME
Pfeifer
D000480:Alkynes / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D002417:Cattle; D057930:Click Chemistry; D055767:Immobilized Proteins / Q000737:chemistry; D007118:Immunoassay; D010455:Peptides / Q000737:chemistry*; D040081:Protein Array Analysis; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D055767:Immobilized Proteins; D010455:Peptides; D012710:Serum Albumin, Bovine
Azadibenzocyclooctyne (ADIBO ); Catalyst-free; Click chemistry; Immunoassays; Peptide microarrays; Strain-promoted alkyne-azide cycloaddition (SPAAC ); c-Myc peptide