Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR.
Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA was isolated from these samples, and Doppel mRNA was quantified by real-time RT-PCR using SYBR Green. Doppel mRNA expression was higher in the ovary, hypothalamus and brain than in other tissues, and it significantly differed between the two genotypes in brain, ileum, cecum, rectum, colon, and uterus. This study demonstrated that Doppel mRNA expression in sheep with ARR/ARQ or ARH/ARQ genotypes was very different. These findings could be helpful in future studies of the relationship between PrP and Doppel.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
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C
Wang
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
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CL
Zhao
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
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L
Liu
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China wurun@gsau.edu.cn.
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R
Wu
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
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Zhang
D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D005838:Genotype; D011328:Prions / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012756:Sheep / Q000235:genetics*
D011328:Prions; D012333:RNA, Messenger