Periosteum Metabolism and Nerve Fiber Positioning Depend on Interactions between Osteoblasts and Peripheral Innervation in Rat Mandible.
The sympathetic nervous system controls bone remodeling by regulating bone formation and resorption. How nerves and bone cells influence each other remains elusive. Here we modulated the content or activity of the neuropeptide Vasoactive Intestinal Peptide to investigate nerve-bone cell interplays in the mandible periosteum by assessing factors involved in nerve and bone behaviors. Young adult rats were chemically sympathectomized or treated with Vasoactive Intestinal Peptide or Vasoactive Intestinal Peptide10-28, a receptor antagonist. Sympathectomy depleted the osteogenic layer of the periosteum in neurotrophic proNerve Growth Factor and neurorepulsive semaphorin3a; sensory Calcitonin-Gene Related Peptide-positive fibers invaded this layer physiologically devoid of sensory fibers. In the periosteum non-osteogenic layer, sympathectomy activated mast cells to release mature Nerve Growth Factor while Calcitonin-Gene Related Peptide-positive fibers increased. Vasoactive Intestinal Peptide treatment reversed sympathectomy effects. Treating intact animals with Vasoactive Intestinal Peptide increased proNerve Growth Factor expression and stabilized mast cells. Vasoactive Intestinal Peptide10-28 treatment mimicked sympathectomy effects. Our data suggest that sympathetic Vasoactive Intestinal Peptide modulate the interactions between nervous fibers and bone cells by tuning expressions by osteogenic cells of factors responsible for mandible periosteum maintenance while osteogenic cells keep nervous fibers at a distance from the bone surface.
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France; Assistance Publique - Hôpitaux de Paris, Avenue Victoria, Paris, France.
Cédric
C
Mauprivez
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France; Assistance Publique - Hôpitaux de Paris, Avenue Victoria, Paris, France.
Caroline
C
Bataille
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France.
Brigitte
B
Baroukh
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France.
Annie
A
Llorens
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France.
Julie
J
Lesieur
UMR-1132 INSERM and Université Paris Diderot, Sorbonne Paris Cité, Hôpital Lariboisière, Paris, France.
Pierre J
PJ
Marie
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France; Assistance Publique - Hôpitaux de Paris, Avenue Victoria, Paris, France.
Jean-Louis
JL
Saffar
Assistance Publique - Hôpitaux de Paris, Avenue Victoria, Paris, France; INSERM U1163, Université Paris Descartes, Sorbonne Paris Cité, Institut Imagine, Hôpital Necker-Enfants Malades, Paris, France.
Martin
M
Biosse Duplan
EA2496 Laboratoire Pathologies, Imagerie et Biothérapies oro-faciales, Faculté de Chirurgie Dentaire, Université Paris Descartes, Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, Montrouge, France; Assistance Publique - Hôpitaux de Paris, Avenue Victoria, Paris, France.
Marc
M
Cherruau
D000818:Animals; D008297:Male; D008334:Mandible / Q000187:drug effects / Q000294:innervation*; D009412:Nerve Fibers / Q000187:drug effects / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D010521:Periosteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D009414:Nerve Growth Factors; D014660:Vasoactive Intestinal Peptide