Genetic variation and geographic differentiation among populations of the nonmigratory agricultural pest Oedaleus infernalis (Orthoptera: Acridoidea) in China.
The nonmigratory grasshopper Oedaleus infernalis Saussure (Orthoptera : Acridoidea) is an agricultural pest to crops and forage grasses over a wide natural geographical distribution in China. The genetic diversity and genetic variation among 10 geographically separated populations of O. infernalis was assessed using polymerase chain reaction-based molecular markers, including the intersimple sequence repeat and mitochondrial cytochrome oxidase sequences. A high level of genetic diversity was detected among these populations from the intersimple sequence repeat (H: 0.2628, I: 0.4129, Hs: 0.2130) and cytochrome oxidase analyses (Hd: 0.653). There was no obvious geographical structure based on an unweighted pair group method analysis and median-joining network. The values of FST, θ(II), and Gst estimated in this study are low, and the gene flow is high (Nm > 4). Analysis of the molecular variance suggested that most of the genetic variation occurs within populations, whereas only a small variation takes place between populations. No significant correlation was found between the genetic distance and geographical distance. Overall, our results suggest that the geographical distance plays an unimpeded role in the gene flow among O. infernalis populations.
College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China.
Wei
W
Sun
College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China.
Hui
H
Dong
Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China.
Yue-Bo
YB
Gao
College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China Key Laboratory of Integrated Pest Management on Crops in Northeast, Ministry of Agriculture, Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China.
Qian-Fu
QF
Su
College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China.
Hai-Tao
HT
Qian
General Station of Forest Pest Management, State Forestry Administration, Shenyang 110034, China.
Hong-Yan
HY
Bai
School of Environment & Life Science, Kaili University, Kaili 556011, China.
Zhu-Ting
ZT
Zhang
College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China bin1956@163.com.
Bin
B
Cong
D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D051456:Gene Flow; D014644:Genetic Variation; D005843:Geography; D006110:Grasshoppers / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV
COI; ISSR; gene flow; genetic diversity