Deep sequencing of mRNA in CD24(-) and CD24(+) mammary carcinoma Mvt1 cell line.
CD24 is an anchored cell surface marker that is highly expressed in cancer cells (Lee et al., 2009) and its expression is associated with poorer outcome of cancer patients (Kristiansen et al., 2003). Phenotype comparison between two subpopulations derived from the Mvt1 cell line, CD24(-) cells (with no CD24 cell surface expression) and the CD24(+) cells, identified high tumorigenic capacity for the CD24(+) cells. In order to reveal the transcripts that support the CD24(+) aggressive and invasive phenotype we compared the gene profiles of these two subpopulations. mRNA profiles of CD24(-) and CD24(+) cells were generated by deep sequencing, in triplicate, using an Illumina HiSeq 2500. Here we provide a detailed description of the mRNA-seq analysis from our recent study (Rostoker et al., 2015). The mRNA-seq data have been deposited in the NCBI GEO database (accession number GSE68746).
Diabetes and Metabolism Clinical Research Center of Excellence, Clinical Research Institute at Rambam (CRIR), Haifa 31096, Israel ; Faculty of Medicine, Technion, Rambam Medical Center, Haifa 31096, Israel.
Ran
R
Rostoker
Girihlet Inc., 423 West 127th Street, First Floor, NY 10027, United States.
Anitha D
AD
Jayaprakash
Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY 10029, United States.
Ravi
R
Sachidanandam
Departments of Medicine, Endocrinology, Diabetes and Bone Disease, Icahn School of Medicine at Mount Sinai, NY 10029, United States.
Derek
D
LeRoith
CD24; Cancer; MRNA-seq; Mammary; Mvt1 cells