Lipopolysaccharide enhances apoptosis of corpus luteum in isolated perfused bovine ovaries in vitro.
Lipopolysaccharide (LPS), the endotoxin of Gram-negative bacteria, has detrimental effects on the structure and function of bovine corpus luteum (CL) in vivo. The objective was to investigate whether these effects were mediated directly by LPS or via LPS-induced release of PGF2α. Bovine ovaries with a mid-cycle CL were collected immediately after slaughter and isolated perfused for 240 min. After 60 min of equilibration, LPS (0.5 μg/ml) was added to the medium of five ovaries, whereas an additional six ovaries were not treated with LPS (control). After 210 min of perfusion, all ovaries were treated with 500 iu of hCG. In the effluent perfusate, concentrations of progesterone (P4) and PGF2α were measured every 10 and 30 min, respectively. Punch biopsies of the CL were collected every 60 min and used for RT-qPCR to evaluate mRNA expression of receptors for LPS (TLR2, -4) and LH (LHCGR); the cytokine TNFA; steroidogenic (STAR, HSD3B), angiogenic (VEGFA121, FGF2), and vasoactive (EDN1) factors; and factors of prostaglandin synthesis (PGES, PGFS, PTGFR) and apoptosis (CASP3, -8, -9). Treatment with LPS abolished the hCG-induced increase in P4 (P≤0.05); however, there was a tendency (P=0.10) for increased release of PGF2α at 70 min after LPS challenge. Furthermore, mRNA abundance of TLR2, TNFA, CASP3, CASP8, PGES, PGFS, and VEGFA121 increased (P≤0.05) after LPS treatment, whereas all other factors remained unchanged (P>0.05). In conclusion, reduced P4 responsiveness to hCG in LPS-treated ovaries in vitro was not due to reduced steroidogenesis, but was attributed to enhanced apoptosis. However, an impact of luteal PGF2α could not be excluded.
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan jluettgenau@vetclinics.uzh.ch.
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Lüttgenau
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Möller
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Kradolfer
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Wellnitz
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Bruckmaier
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Miyamoto
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Ulbrich
Clinic of Reproductive MedicineVetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandETH ZurichAnimal Physiology, Institute of Agricultural Sciences, Zurich, SwitzerlandVeterinary PhysiologyVetsuisse Faculty, University of Bern, Bern, SwitzerlandGraduate School of Animal and Food HygieneObihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
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Bollwein
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000235:genetics; D053181:Caspase 8 / Q000235:genetics; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000166:cytology* / Q000187:drug effects; D015237:Dinoprost / Q000032:analysis; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006911:Hydroxyprostaglandin Dehydrogenases / Q000235:genetics; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects; D011374:Progesterone / Q000032:analysis; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D006063:Chorionic Gonadotropin; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D011374:Progesterone; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C031051:prostaglandin-F synthase; D053148:Caspase 3; D053181:Caspase 8; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D005947:Glucose