Genome-wide RNA profiling of long-lasting stem cell-like memory CD8 T cells induced by Yellow Fever vaccination in humans.
The live-attenuated Yellow Fever (YF) vaccine YF-17D induces a broad and polyfunctional CD8 T cell response in humans. Recently, we identified a population of stem cell-like memory CD8 T cells induced by YF-17D that persists at stable frequency for at least 25 years after vaccination. The YF-17D is thus a model system of human CD8 T cell biology that furthermore allows to track and study long-lasting and antigen-specific human memory CD8 T cells. Here, we describe in detail the sample characteristics and preparation of a microarray dataset acquired for genome-wide gene expression profiling of long-lasting YF-specific stem cell-like memory CD8 T cells, compared to the reference CD8 T cell differentiation subsets from total CD8 T cells. We also describe the quality controls, annotations and exploratory analyses of the dataset. The microarray data is available from the Gene Expression Omnibus (GEO) public repository with accession number GSE65804.
Ludwig Cancer Center, University of Lausanne, Switzerland ; Department of Oncology, Lausanne University Hospital (CHUV), Switzerland.
Silvia A
SA
Fuertes Marraco
Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.
Charlotte
C
Soneson
Ludwig Cancer Center, University of Lausanne, Switzerland ; Department of Oncology, Lausanne University Hospital (CHUV), Switzerland ; Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.
Mauro
M
Delorenzi
Ludwig Cancer Center, University of Lausanne, Switzerland ; Department of Oncology, Lausanne University Hospital (CHUV), Switzerland.
Daniel E
DE
Speiser