Effects of chlorpyrifos on life cycle parameters, cytochrome P450S expression, and antioxidant systems in the monogonont rotifer Brachionus koreanus.
Chlorpyrifos is a widely used organophosphorus insecticide for controlling diverse insect pests of crops. In the monogonont rotifer Brachionus koreanus, population growth retardation with the inhibition of lifespan, fecundity, and individual body size of ovigerous females was shown over 10 d in response to chlorpyrifos exposure. At the molecular and biochemical levels, the rotifer B. koreanus defensome, composed of cytochrome P450 complements, heat shock protein 70, and antioxidant enzymatic systems (i.e., glutathione, glutathione peroxidase, glutathione reductase, and glutathione S-transferase), was significantly induced in response to different concentrations of chlorpyrifos. Thus, chlorpyrifos strongly induced a defensome system to mitigate the deleterious effects of chlorpyrifos at in vivo and in vitro levels as a trade-off in fitness costs. Environ Toxicol Chem 2016;35:1449-1457. © 2015 SETAC.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, South Korea.
Ryeo-Ok
RO
Kim
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, South Korea.
Bo-Mi
BM
Kim
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, South Korea.|Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.
Chang-Bum
CB
Jeong
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, South Korea.
Jae-Seong
JS
Lee
Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon, South Korea.
Jae-Sung
JS
Rhee
D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004390:Chlorpyrifos / Q000378:metabolism / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D020869:Gene Expression Profiling; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008018:Life Cycle Stages / Q000187:drug effects*; D012403:Rotifera / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
D000975:Antioxidants; D004785:Environmental Pollutants; D003577:Cytochrome P-450 Enzyme System; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D004390:Chlorpyrifos
Antioxidant; Chlorpyrifos; Cytochrome P450; Marine environmental monitoring; Rotifer