GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L).
NAC (NAM, ATAF, and CUC) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. Currently, 77 NAC genes have been reported in cotton (Gossypium hirsutum L.). And GhNAC12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. In the present study, a preliminary function analysis of GhNAC12 was performed during leaf senescence. qRT-PCR analysis indicated that GhNAC12 expression increased during the early-aging process and the aging of cotyledons. Additionally, we observed that overexpression of GhNAC12 in Arabidopsis led to early senescence (early aging). Our findings suggest that GhNAC12 is a candidate gene for early aging in upland cotton cultivars. Neutrality tests suggested that there was no selection pressure imposed on GhNAC12 during the domestication of upland cotton.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong, PR China.
Fengli
F
Zhao
College of Life Sciences, Henan Normal University, Xinxiang, Henan, PR China.
Jianhui
J
Ma
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
Libei
L
Li
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
Shuli
S
Fan
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
Yaning
Y
Guo
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
Meizhen
M
Song
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China.
Hengling
H
Wei
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China. Electronic address: chypang@163.com.
Chaoyou
C
Pang
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, PR China. Electronic address: yu@cricaas.com.cn.
Shuxun
S
Yu
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D013997:Time Factors; D024363:Transcription Initiation Site
D010940:Plant Proteins; D002734:Chlorophyll
Bioinformatics analysis; GhNAC12; Gossypium hirsutum L; Leaf senescence; Neutrality tests; qRT-PCR