A local redox cycling-based electrochemical chip device with nanocavities for multi-electrochemical evaluation of embryoid bodies.
An electrochemical device, which consists of electrode arrays, nanocavities, and microwells, was developed for multi-electrochemical detection with high sensitivity. A local redox cycling-based electrochemical (LRC-EC) system was used for multi-electrochemical detection and signal amplification. The LRC-EC system consists of n(2) sensors with only 2n bonding pads for external connection. The nanocavities fabricated in the sensor microwells enable significant improvement of the signal amplification compared with the previous devices we have developed. The present device was successfully applied for evaluation of embryoid bodies (EBs) from embryonic stem (ES) cells via electrochemical measurements of alkaline phosphatase (ALP) activity in the EBs. In addition, the EBs were successfully trapped in the sensor microwells of the device using dielectrophoresis (DEP) manipulation, which led to high-throughput cell analysis. This device is considered to be useful for multi-electrochemical detection and imaging for bioassays including cell analysis.
Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp.
Yusuke
Y
Kanno
Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp.
Kosuke
K
Ino
Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp.
Hitoshi
H
Shiku
Graduate School of Environmental Studies, Tohoku University, Japan. ino.kosuke@bioinfo.che.tohoku.ac.jp matsue@bioinfo.che.tohoku.ac.jp and WPI-Advanced Institute for Materials Research, Tohoku University, Japan.
Tomokazu
T
Matsue
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002138:Calibration; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D058732:Embryoid Bodies / Q000201:enzymology / Q000378:metabolism*; D004867:Equipment Design; D051379:Mice; D036103:Nanotechnology / Q000295:instrumentation*; D010084:Oxidation-Reduction
D000469:Alkaline Phosphatase