Sequence variation of Bemisia tabaci Chemosensory Protein 2 in cryptic species B and Q: New DNA markers for whitefly recognition.
Bemisia tabaci Gennadius biotypes B and Q are two of the most important worldwide agricultural insect pests. Genomic sequences of Type-2 B. tabaci chemosensory protein (BtabCSP2) were cloned and sequenced in B and Q biotypes, revealing key biotype-specific variations in the intron sequence. A Q260 sequence was found specifically in Q-BtabCSP2 and Cucumis melo LN692399, suggesting ancestral horizontal transfer of gene between the insect and the plant through bacteria. A cleaved amplified polymorphic sequences (CAPS) method was then developed to differentiate B and Q based on the sequence variation in exon of BtabCSP2 gene. The performances of CSP2-based CAPS for whitefly recognition were assessed using B. tabaci field collections from Shandong Province (P.R. China). Our SacII based CAPS method led to the same result compared to mitochondrial cytochrome oxidase-based CAPS method in the field collections. We therefore propose an explanation for CSP origin and a new rapid simple molecular method based on genomic DNA and chemosensory gene to differentiate accurately the B and Q whiteflies of the Bemisia complex around the world.
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Guo-Xia
GX
Liu
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Hong-Mei
HM
Ma
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Hong-Yan
HY
Xie
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Ning
N
Xuan
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China. Electronic address: jfpicimbon@gmail.com.
Jean-François
JF
Picimbon
D000818:Animals; D001483:Base Sequence; D002681:China; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics; D022761:Gene Transfer, Horizontal; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006430:Hemiptera / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D007438:Introns; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny
D005819:Genetic Markers; D019476:Insect Proteins; D003576:Electron Transport Complex IV; C035337:CCGCGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Bemisia tabaci; BtabCSP2; Evolution; Intron; chemosensory protein; cleaved amplified polymorphic sequences