Antagonistic Activities of Novel Peptides from Bacillus amyloliquefaciens PT14 against Fusarium solani and Fusarium oxysporum.
Bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. This paper reports on the antifungal activity of novel peptides isolated from Bacillus amyloliquefaciens PT14. Reverse-phase high-performance liquid chromatography revealed that B. amyloliquefaciens PT14 produces five peptides (PT14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. In particular, PT14-3 and PT14-4a showed broad-spectrum antifungal activity against Fusarium solani and Fusarium oxysporum. The PT14-4a N-terminal amino acid sequence was identified through Edman degradation, and a BLAST homology analysis showed it not to be identical to any other protein or peptide. PT14-4a displayed strong fungicidal activity with minimal inhibitory concentrations of 3.12 mg/L (F. solani) and 6.25 mg/L (F. oxysporum), inducing severe morphological deformation in the conidia and hyphae. On the other hand, PT14-4a had no detectable hemolytic activity. This suggests PT14-4a has the potential to serve as an antifungal agent in clinical therapeutic and crop-protection applications.
Department of Biomedical Science, Chosun University , Gwangju 61452, Korea.
Young Gwon
YG
Kim
Department of Biomedical Science, Chosun University , Gwangju 61452, Korea.
Hee Kyoung
HK
Kang
Department of Bioinformatics, Kongju National University , Kongju 32588, Korea.
Kee-Deok
KD
Kwon
Department of Bioinformatics, Kongju National University , Kongju 32588, Korea.
Chang Ho
CH
Seo
College of Agriculture and Life Sciences, Chonnam National University , Gwangju 61186, Korea.
Hyang Burm
HB
Lee
Department of Biomedical Science, Chosun University , Gwangju 61452, Korea.|Research Center for Proteineous Materials, Chosun University , Gwangju 61452, Korea.
Yoonkyung
Y
Park
D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects*; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D000935:Antifungal Agents; D010455:Peptides
Bacillus amyloliquefaciens; Fusarium oxysporum; Fusarium solani; antifungal peptide; biological control