Effects of Panax ginseng extract on human dermal fibroblast proliferation and collagen synthesis.
Current studies of Panax ginseng (or Korean ginseng) have demonstrated that it has various biological effects, including angiogenesis, immunostimulation, antimicrobial and anti-inflammatory effects. Therefore, we hypothesised that P. ginseng may also play an important role in wound healing. However, few studies have been conducted on the wound-healing effects of P. ginseng. Thus, the purpose of this in vitro pilot study was to determine the effects of P. ginseng on the activities of fibroblasts, which are key wound-healing cells. Cultured human dermal fibroblasts were treated with one of six concentrations of P. ginseng: 0, 1, 10 and 100 ng/ml and 1 and 10 µg/ml. Cell proliferation was determined 3 days post-treatment using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, and collagen synthesis was evaluated by the collagen type I carboxy-terminal propeptide method. Cell proliferation levels and collagen synthesis were compared among the groups. The 10 ng/ml to 1 µg/ml P. ginseng treatments significantly increased cell proliferation, and the 1 ng/ml to 1 µg/ml concentrations significantly increased collagen synthesis. The maximum effects for both parameters were observed at 10 ng/ml. P. ginseng stimulated human dermal fibroblast proliferation and collagen synthesis at an optimal concentration of 10 ng/ml.
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Geum-Young
GY
Lee
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Kang-Gyun
KG
Park
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Sik
S
Namgoong
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Seung-Kyu
SK
Han
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Seong-Ho
SH
Jeong
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Eun-Sang
ES
Dhong
Department of Plastic Surgery, Korea University College of Medicine, Seoul, Korea.
Woo-Kyung
WK
Kim
D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D005894:Panax; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D003094:Collagen
Fibroblast; Ginseng; Panax ginseng; Wound healing