Toxic Takifugu pardalis eggs found in Takifugu niphobles gut: Implications for TTX accumulation in the pufferfish.
Pufferfish (Takifugu spp.) possess a potent neurotoxin, tetrodotoxin (TTX). TTX has been detected in various organisms including food animals of pufferfish, and TTX-producing bacteria have been isolated from these animals. TTX in marine pufferfish accumulates in the pufferfish via the food web starting with marine bacteria. However, such accumulation is unlikely to account for the amount of TTX in the pufferfish body because of the minute amounts of TTX produced by marine bacteria. Therefore, the toxification process in pufferfish still remains unclear. In this article we report the presence of numerous Takifugu pardalis eggs in the intestinal contents of another pufferfish, Takifugu niphobles. The identity of T. pardalis being determined by direct sequencing for mitochondrial DNA. LC-MS/MS analysis revealed that the peak detected in the egg samples corresponded to TTX. Toxification experiments in recirculating aquaria demonstrated that cultured Takifugu rubripes quickly became toxic upon being fed toxic (TTX-containing) T. rubripes eggs. These results suggest that T. niphobles ingested the toxic eggs of another pufferfish T. pardalis to toxify themselves more efficiently via a TTX loop consisting of TTX-bearing organisms at a higher trophic level in the food web.
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan. Electronic address: sitoi@nihon-u.ac.jp.
Shiro
S
Itoi
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Ao
A
Kozaki
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Keitaro
K
Komori
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Tadasuke
T
Tsunashima
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Shunsuke
S
Noguchi
Department of Sea-Farming, Aichi Fish Farming Institute, Tahara, Aichi 441-3618, Japan.
Mitsuo
M
Kawane
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Haruo
H
Sugita
D000818:Animals; D005247:Feeding Behavior; D005260:Female; D020387:Food Chain; D008297:Male; D010063:Ovum / Q000737:chemistry*; D010802:Phylogeny; D023741:Takifugu / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013779:Tetrodotoxin / Q000032:analysis / Q000378:metabolism*
D013779:Tetrodotoxin
Congeneric eggs; Food chain; Pufferfish; Takifugu niphobles; Takifugu pardalis; Tetrodotoxin