Coacervation of Elastin-Like Recombinamer Microgels.
The investigation of the coacervation (self-aggregation) behavior of biomicrogels which can potentially be used as drug carriers is an important topic, because self-aggregation can not only cause loss of activity, but also toxicity and immunogenicity. To study this effect microgels from elastin-like recombinamer are synthesized using miniemulsion technique. The existence of coacervation for such microgels, at different concentrations and temperatures, is studied and proved by cryo-field emission scanning clectron microscopy (cryo-FESEM), cryo-transmission electron microscopy (cryo-TEM), and by a novel (1) H high-resolution magic angle sample spinning (HRMAS), nuclear magnetic resonance (NMR) spectroscopy, and relaxometry methods. The findings by (1) H HRMAS NMR spectroscopy and relaxometry show simultaneous processes of volume phase temperature transition and coacervation with different sensitivity for hydrophobic and hydrophilic amino acid side-chains in the microgel. The coacervation process is more evidential by the behavior of glycine α-CH2 , (1) H NMR peak as compared to the proline β-CH2 .
DWI-Leibniz-Institute for Interactive Materials, e.V., RWTH-Aachen University, Forckenbeckstraße 50, D-52074, Aachen, Germany.
Smriti
S
Singh
DWI-Leibniz-Institute for Interactive Materials, e.V., RWTH-Aachen University, Forckenbeckstraße 50, D-52074, Aachen, Germany.|Department of Physics and Chemistry, Technical University of Cluj-Napoca, 25 G. Baritiu Str., RO-400027, Cluj-Napoca, Romania.
Dan Eugen
DE
Demco
DWI-Leibniz-Institute for Interactive Materials, e.V., RWTH-Aachen University, Forckenbeckstraße 50, D-52074, Aachen, Germany.
Khosrow
K
Rahimi
Department of Physics and Chemistry, Technical University of Cluj-Napoca, 25 G. Baritiu Str., RO-400027, Cluj-Napoca, Romania.
Radu
R
Fechete
G. I. R. Bioforge, University of Valladolid, CIBER-BBN, Valladolid, 47011, Spain.
José Carlos
JC
Rodriguez-Cabello
DWI-Leibniz-Institute for Interactive Materials, e.V., RWTH-Aachen University, Forckenbeckstraße 50, D-52074, Aachen, Germany.
Martin
M
Möller
D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers; D004549:Elastin / Q000737:chemistry; D004655:Emulsions; D005427:Flocculation; D005782:Gels; D057927:Hydrophobic and Hydrophilic Interactions; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D044367:Phase Transition; D013696:Temperature; D013816:Thermodynamics
D004337:Drug Carriers; D004655:Emulsions; D005782:Gels; D009842:Oligopeptides; D004549:Elastin
1H NMR relaxometry; 1H NMR spectroscopy; biohybrids; coacervation; elastins; electron micrography; microgels; recombinant elastin