Micropatch-arrayed pads for non-invasive spatial and temporal profiling of topical drugs on skin surface.
Micropatch-arrayed pads (MAPAs) are presented as a facile and sensitive sampling method for spatial profiling of topical agents adsorbed on the surface of skin. MAPAs are 28 × 28 mm sized pieces of polytetrafluoroethylene containing plurality of cavities filled with agarose hydrogel. They are affixed onto skin for 10 min with the purpose to collect drugs applied topically. Polar compounds are absorbed by the hydrogel micropatches. The probes are subsequently scanned by an automated nanospray desorption electrospray ionization mass spectrometry system operated in the tapping dual-polarity mode. When the liquid junction gets into contact with every micropatch, polar compounds absorbed in the hydrogel matrix are desorbed and transferred to the ion source. A 3D-printed interface prevents evaporation of hydrogel micropatches assuring good reproducibility and sensitivity. MAPAs have been applied to follow dispersion of topical drugs applied to human skin in vivo and to porcine skin ex vivo, in the form of self-adhesive patches. Spatiotemporal characteristics of the drug dispersion process have been revealed using this non-invasive test. Differences between drug dispersion in vivo and ex vivo could be observed. We envision that MAPAs can be used to investigate spatiotemporal kinetics of various topical agents utilized in medical treatment.
Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd., Hsinchu, 300, Taiwan.
Ewelina P
EP
Dutkiewicz
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, 1 Jen-Ai Rd., Taipei, 100, Taiwan.|Department of Dermatology, National Taiwan University Hospital Hsinchu Branch, 25 Jingguo Rd., Hsinchu, 300, Taiwan.|Department of Dermatology, National Taiwan University Hospital, 7 Chung San South Rd., Taipei, 100, Taiwan.|College of Medicine, National Taiwan University, 1 Jen-Ai Rd., Taipei, 100, Taiwan.
Hsien-Yi
HY
Chiu
Department of Applied Chemistry, National Chiao Tung University, 1001 University Rd., Hsinchu, 300, Taiwan.|Institute of Molecular Science, National Chiao Tung University, 1001 University Rd., Hsinchu, 300, Taiwan.
Pawel L
PL
Urban
D000287:Administration, Topical; D000818:Animals; D004867:Equipment Design; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D057230:Limit of Detection; D009538:Nicotine / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000032:analysis*; D066330:Printing, Three-Dimensional; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis; D012867:Skin; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D057968:Transdermal Patch
D004364:Pharmaceutical Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009538:Nicotine; D012601:Scopolamine
hydrogel; mass spectrometry; skin metabolites; sweat; topical drug