Genome-wide DNA binding pattern of two-component system response regulator RhpR in Pseudomonas syringae.
Although Pseudomonas syringae uses the two-component system RhpRS to modulate the expression of type III secretion system (T3SS) genes and pathogenicity, the molecular mechanisms and the regulon of RhpRS have yet to be fully demonstrated. We have performed a genome-wide analysis of RhpR binding to DNA prepared from P. syringae pv. phaseolicola in order to identify candidate direct targets of RhpR-mediated transcriptional regulation, as described in our recent article [1]. The data are available from NCBI Gene Expression Omnibus (GEO) with the accession number GSE58533. Here we describe the detailed methods and data analyses of our RhpR ChIP-seq dataset.
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, PR China ; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, PR China.
Tianhong
T
Zhou
Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Kai
K
Chen
Lanpec Technologies Co., Ltd., No. 8 Lanke Rd. Anning District, Lanzhou, Gansu 730070, PR China.
Hai-Xin
HX
Zhang
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457, PR China ; Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin 300071, PR China.
Xin
X
Deng
ChIP-seq; Pseudomonas syringae; Two-component system