When molecules support morphology: Phylogenetic reconstruction of the family Onuphidae (Eunicida, Annelida) based on 16S rDNA and 18S rDNA.
Onuphid polychaetes are tubicolous marine worms commonly reported worldwide from intertidal areas to hadal depths. They often dominate in benthic communities and have economic importance in aquaculture and recreational fishing. Here we report the phylogeny of the family Onuphidae based on the combined analyses of nuclear (18S rDNA) and mitochondrial (16S rDNA) genes. Results of Bayesian and Maximum Likelihood analyses supported the monophyly of Onuphidae and its traditional subdivision into two monophyletic subfamilies: Onuphinae and Hyalinoeciinae. Ten of 22 recognized genera were monophyletic with strong node support; four more genera included in this study were either monotypic or represented by a single species. None of the genera appeared para- or polyphyletic and this indicates a strong congruence between the traditional morphology-based systematics of the family and the newly obtained molecular-based phylogenetic reconstructions. Intergeneric relationships within Hyalinoeciinae were not resolved. Two strongly supported monophyletic groups of genera were recovered within Onuphinae: ((Onuphis, Aponuphis), Diopatra, Paradiopatra) and (Hirsutonuphis, (Paxtonia, (Kinbergonuphis, Mooreonuphis))). A previously accepted hypothesis on the subdivision of Onuphinae into the Onuphis group of genera and the Diopatra group of genera was largely rejected.
Natural History Collections, University Museum of Bergen, P.O. Box 7800, NO-5020 Bergen, Norway; P.P. Shirshov Institute of Oceanology, Russian Academy of Sciences, Nakhimovsky Pr. 36, 117997 Moscow, Russia. Electronic address: nataliya.budaeva@uib.no.
Nataliya
N
Budaeva
Evolutionary Genomics Laboratory, Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskiye Gory, 1 Build. 12, 119991 Moscow, Russia; Koltzov Institute of Developmental Biology RAS, Vavilov Str. 26, 119334 Moscow, Russia; National Research University Higher School of Economics, Myasnitskaya Str. 20, 101000 Moscow, Russia.
Dmitry
D
Schepetov
Universidade Federal do Rio de Janeiro, Polo Xerém, Estrada de Xerém 27, Duque de Caxias, RJ 25250-470, Brazil.
Joana
J
Zanol
White Sea Biological Station, Faculty of Biology, Lomonosov Moscow State University, Leninskiye Gory, 1 Build. 12, 119991 Moscow, Russia; Pirogov Russian National Research Medical University, Ostrovityanova Str. 1, Moscow 117997, Russia.
Tatiana
T
Neretina
Natural History Collections, University Museum of Bergen, P.O. Box 7800, NO-5020 Bergen, Norway.
Endre
E
Willassen
D000818:Animals; D000845:Annelida / Q000145:classification* / Q000235:genetics; D004275:DNA, Ribosomal; D058889:Molecular Typing; D010802:Phylogeny; D011077:Polychaeta / Q000145:classification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S
Beach worms; Evolution; Hyalinoeciinae; Onuphinae; Quill worms; Systematics