The changes in springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum and Biceps femoris muscles during the rigour period.
This study describes the changes taking place during rigour in springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum (LTL) and Biceps femoris (BF) muscles. Samples from six male and six female springbok were snap-frozen at 2, 3, 5, 8, 12, 18, 24 and 30h post-mortem (PM) and the pH, calpains I, II and calpastatin activities and cathepsins B, BL and H activities were determined. The temperature was also recorded. Significant third-order interactions were found for the pH and temperature, with the female LTL cooling more rapidly and acidifying slower than the other samples. Female muscles were at risk of developing cold-shortening and all the samples cooled more rapidly than recommended for cattle or sheep. Cathepsin BL activity increased PM, likely due to the degradation of the lysosomes. Calpains I, II and calpastatin activity declined during rigour, indicating that the calpains were activated early PM. Gender and muscle had a significant effect on calpain and cathepsin activity.
Department of Animal Sciences, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.
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MK
North
Agricultural Research Council of South Africa, Private Bag X2, Irene 0062, South Africa.
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L
Frylinck
Department of Animal Sciences, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa. Electronic address: lch@sun.ac.za.
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LC
Hoffman
D000818:Animals; D000866:Antelopes / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism; D002403:Cathepsins / Q000378:metabolism; D055598:Chemical Phenomena; D005260:Female; D063427:Food Quality; D061353:Food Storage; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008460:Meat / Q000032:analysis*; D055595:Mechanical Phenomena; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D059748:Proteolysis; D012034:Refrigeration; D012298:Rigor Mortis / Q000201:enzymology / Q000378:metabolism / Q000662:veterinary*; D012727:Sex Characteristics; D013019:South Africa; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000201:enzymology / Q000378:metabolism / Q000503:physiopathology
D002135:Calcium-Binding Proteins; D007527:Isoenzymes; C033668:calpastatin; D002403:Cathepsins; D002154:Calpain
Calpain; Cathepsin; Cold-shortening; Game meat; Temperature; pH