Multi-residue fluorescent microspheres immunochromatographic assay for simultaneous determination of macrolides in raw milk.
A rapid, reliable, sensitive, and quantitative multi-residue fluorescent microspheres immunochromatographic assay (FMCA) was developed for simultaneous detection of four macrolides in raw milk. The IC50 value of the optimized FMCA was 1.36, 1.22, 1.01, and 1.39 ng/mL for erythromycin (ERY), spiramycin (SPI), tilmicosin (TIM), and tylosin (TYL), respectively. The limits of detection (LODs) for the four macrolides was 0.13 ng/mL. The recoveries of ERY, SPI, TIM, and TYL from spiked raw milk ranged from 91.8-109.2, 89.6-114.4, 84.8-111.6, and 85.8-115.2%, respectively, with coefficients of variation (CVs) of 5.4-11.3, 7.9-15.7, 6.2-13.7, and 3.2-14.9%, respectively. The whole testing process was completed within 20 min. The antibody-mixed labeled method was successfully applied to the FMCA, which greatly simplified the operation steps and saved a lot of time. Compared with the immunogold chromatographic assay (IGCA), the FMCA is more sensitive and stable and has less antibody consumption. A parallel analysis in blind raw milk samples was conducted by liquid chromatography-tandem mass spectrometry (LC-MS/MS); the results showed good correlation (r(2) = 0.99) between the two methods. Therefore, the developed multi-residue FMCA is reliable and can be easily applied to other antibiotics or other contaminants.
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China.|Beijing WDWK Biotechnology Co., Ltd., Beijing, 100085, China.
Xiangmei
X
Li
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China.
Jianzhong
J
Shen
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China.
Qi
Q
Wang
Beijing WDWK Biotechnology Co., Ltd., Beijing, 100085, China.
Shuxia
S
Gao
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China.
Xingyao
X
Pei
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China.
Haiyang
H
Jiang
College of Veterinary Medicine, China Agricultural University, Beijing, 100093, China. wenkai@cau.edu.cn.
Kai
K
Wen
D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods*; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000737:chemistry* / Q000302:isolation & purification*; D005453:Fluorescence; D005506:Food Contamination; D018942:Macrolides / Q000737:chemistry* / Q000302:isolation & purification*; D008863:Microspheres; D008892:Milk / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D000900:Anti-Bacterial Agents; D018942:Macrolides
Fluorescent microspheres; Immunochromatographic assay; Macrolide; Raw milk