Three capsid amino acids notably influence coxsackie B3 virus stability.
Coxsackievirus B3 strain 28 (CVB3/28) is less stable at 37 °C than eight other CVB3 strains with which it has been compared, including four in this study. In a variant CVB3/28 population selected for increased stability at 37 °C, the capsid proteins of the stable variant differed from the parental CVB3/28 by two mutations in Vp1 and one mutation in Vp3, each of which resulted in altered protein sequences. Each of the amino acid changes was individually associated with a more stable virus. Competition between CVB3/28 and a more stable derivative of the strain showed that propagation of the less stable virus was favoured in receptor-rich HeLa cells.
Department of Pathology and Microbiology, University of Nebraska College of Medicine, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.
Steven D
SD
Carson
Department of Pathology and Microbiology, University of Nebraska College of Medicine, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.
Steven
S
Tracy
Department of Pathology and Microbiology, University of Nebraska College of Medicine, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.
Zac G
ZG
Kaczmarek
Department of Microbiology, College of Medicine, Jazan University, King Abdullah Street, Jazan 82621, Saudi Arabia.
Abdulaziz
A
Alhazmi
Department of Pathology and Microbiology, University of Nebraska College of Medicine, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA.
Nora M
NM
Chapman
D000596:Amino Acids / Q000032:analysis*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D029822:Enterovirus B, Human / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects*; D004847:Epithelial Cells / Q000821:virology; D006367:HeLa Cells; D006801:Humans; D050296:Microbial Viability / Q000528:radiation effects*; D050505:Mutant Proteins / Q000235:genetics; D020125:Mutation, Missense; D013696:Temperature; D053585:Virus Attachment
D000596:Amino Acids; D036022:Capsid Proteins; D050505:Mutant Proteins