First Pregnancy, Somatic and Psychological Status of a 4-Year-Old Child Born following Annexin V TESA Sperm Separation.
Introduction Sperm DNA integrity is a crucial paternal factor affecting fertilization and pregnancy rates, as well as embryo development. Case The present case report describes the successful pregnancy after testicular sperm aspiration (TESA) combined with intracytoplasmic sperm injection (ICSI) (TESA-ICSI) in a couple where the male presented high sperm DNA fragmentation. In order to sort damaged sperm presenting DNA fragmentation, magnetic activated cell sorting (MACS) with annexin V microbeads (MACS Miltenyi Biotec, Teterow, Germany) was used. Conclusion The authors present the first description of a successful medical case using TESA-ICSI annexin V sperm sorting. Additionally, a follow-up of the child at the age of 4 years old was done.
INVICTA Fertility and Reproductive Centre, Gdańsk, Poland ; INVICTA Fertility and Reproductive Centre, Warsaw, Poland ; Department of Obstetrics and Gynecological Nursing, Medical University of Gdansk, Gdańsk, Poland ; Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland.
Krzysztof
K
Lukaszuk
INVICTA Fertility and Reproductive Centre, Warsaw, Poland.
Monika
M
Wcislo
INVICTA Fertility and Reproductive Centre, Gdańsk, Poland.
Joanna
J
Liss
Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland.
Anna
A
Stachowicz
Department of Obstetrics and Gynaecology, The Medical Center of Postgraduate Education, Warsaw, Poland.
Grzegorz
G
Jakiel
INVICTA Fertility and Reproductive Centre, Gdańsk, Poland.
Mariusz
M
Lukaszuk
INVICTA Fertility and Reproductive Centre, Gdańsk, Poland ; Department of Obstetrics and Gynecological Nursing, Medical University of Gdansk, Gdańsk, Poland.
Ewa
E
Pastuszek
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland.
Izabela
I
Woclawek-Potocka
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland.
Antonio
A
Galvao
INVICTA Fertility and Reproductive Centre, Gdańsk, Poland.
Dorota
D
Bialobrzeska
intracytoplasmic sperm injection; sperm; sperm DNA integrity; testicular sperm aspiration