Construction of a novel twin-arginine translocation (Tat)-dependent type expression vector for secretory production of heterologous proteins in Corynebacterium glutamicum.
Corynebacterium glutamicum is recognized as a favorable host for the secretory production of heterologous proteins. However, there are few secretion-type expression vectors available for protein production in C. glutamicum. In this study, we constructed a shuttle expression vector pAU3, which harbors the strong promoter tac-M for constitutive gene transcription, the consensus RBS sequence for protein translation, and the strong cgR_0949 signal sequence for protein secretion via the Tat pathway in C. glutamicum. The applicability of pAU3 was confirmed by the highly efficient expression and secretion of the CAT protein in C. glutamicum. The vector pAU3 is highly useful for secretory production of heterologous proteins in C. glutamicum.
College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China.
Lirong
L
Zhang
College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China.
Huimin
H
Jia
College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China. Electronic address: daqingxu2013@126.com.
Daqing
D
Xu
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D021382:Protein Sorting Signals / Q000235:genetics; D000069294:Twin-Arginine-Translocation System / Q000235:genetics*
D021382:Protein Sorting Signals; D000069294:Twin-Arginine-Translocation System; D015500:Chloramphenicol O-Acetyltransferase
Corynebacterium glutamicum; Expression vector; Protein secretion; Twin-arginine translocation (Tat); pAU3