Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans.
Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues. We compare the utility of these markers between pig and human lungs to validate translational relevance of each marker. Our results suggest these markers can be a useful addition in the pathological evaluation of porcine models of human disease.
Department of Pathology, University of Iowa, Iowa City, IA, USA david-meyerholz@uiowa.edu.
David K
DK
Meyerholz
Department of Pathology, University of Iowa, Iowa City, IA, USA.
Allyn M
AM
Lambertz
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.
Leah R
LR
Reznikov
Department of Pathology, University of Iowa, Iowa City, IA, USA.
Georgina K
GK
Ofori-Amanfo
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.
Phil H
PH
Karp
Department of Pediatrics, University of Iowa, Iowa City, IA, USA.
Paul B
PB
McCray
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA Department of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA, USA Howard Hughes Medical Institute, University of Iowa, Iowa City, IA, USA.
Michael J
MJ
Welsh
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA Department of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA, USA Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USA.
David A
DA
Stoltz
D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008168:Lung / Q000737:chemistry / Q000000981:diagnostic imaging / Q000473:pathology; D011014:Pneumonia / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D015203:Reproducibility of Results; D013552:Swine
D015415:Biomarkers
human; immunohistochemistry; leukocytes; lung; markers; mucus; pig