[Analysis of Prokaryotic Community Structure in River Waters of the Ningbo Sanjiang Mouth].
The prokaryotic community structure in river waters of the Ningbo Sanjiang Mouth was analyzed for the first time using 16S rRNA gene based-Illumina Miseq high-throughput sequencing. A total of 215 504 high-quality sequences were obtained, and the results of alpha-diversity analysis revealed that Yongjiang River Watershed (YRW) harbored high diversity and richness of prokaryotic communities. Taxonomic assignment analysis indicated that β-Proteobacterium, Actinobacteria and Bacteroidetes dominated in the river water of YRW, and accounted for 78. 88% of the total prokaryotic communities. Hydrological condition may play an important role in influencing the composition and structure of YRW prokaryotic community. In addition, several kinds of sewer- and fecal-pollution indicator bacterial groups were observed in this area with the highest abundance of pollution indicator bacteria occurring in the water sample of Yuyao River, implying that the Yuyao River might have a high potential risk of sewer- and fecal-pollution. Moreover, a total of 76 species and 18 subspecies of potential pathogenic bacteria, which accounted for 2. 19% and 0. 40% of total sequences respectively, were identified using BLASTN analysis with a local pathogenic bacteria database. Overall, this study provided an important basic data for shedding light on the structure and ecological function of YRW prokaryotic community.
An-yi
AY
Hu
Jiang-wei
JW
Li
Xiao-yong
XY
Yang
Hong-jie
HJ
Wang
Chang-ping
CP
Yu
D039903:Actinobacteria; D001419:Bacteria / Q000145:classification*; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004787:Environmental Pollution / Q000032:analysis*; D005243:Feces / Q000382:microbiology; D059014:High-Throughput Nucleotide Sequencing; D020560:Proteobacteria; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S