miRNA Stability in Frozen Plasma Samples.
MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at -80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. mariafrancesca22@virgilio.it.
Francesca
F
Balzano
Associazione "L'Isola dei Centenari", Via Milano 4, Sassari 07100, Italy. isoladeicentenari@gmail.com.
Marta
M
Deiana
Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, Sassari 07100, Italy. silvia.deigiudici@izs-sardegna.it.
Silvia
S
Dei Giudici
Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, Sassari 07100, Italy. annalisa.oggiano@izs-sardegna.it.
Annalisa
A
Oggiano
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. angela.b3@virgilio.it.
Angela
A
Baralla
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. sarapasella18@gmail.com.
Sara
S
Pasella
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. amannu@tiscali.it.
Andrea
A
Mannu
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. mariopescatori@gmail.com.
Mario
M
Pescatori
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. baingio@gmail.com.
Baingio
B
Porcu
Department of Clinical and Experimental Medicine, University of Sassari, vl. San Pietro 8, Sassari 07100, Italy. gfanciu@uniss.it.
Giuseppe
G
Fanciulli
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. azinellu@uniss.it.
Angelo
A
Zinellu
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. carru@uniss.it.
Ciriaco
C
Carru
Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy. proflucadeiana@gmail.com.|Associazione "L'Isola dei Centenari", Via Milano 4, Sassari 07100, Italy. proflucadeiana@gmail.com.
Luca
L
Deiana
D000328:Adult; D001793:Blood Preservation; D015925:Cryopreservation; D020869:Gene Expression Profiling / Q000379:methods*; D006207:Half-Life; D064368:Healthy Volunteers; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry*; D008875:Middle Aged; D020871:RNA Stability
D035683:MicroRNAs
long freezing times; miRNA; sequences AU or UA; stability