Reactive oxygen species involved in apoptosis induction of human respiratory epithelial (A549) cells by Streptococcus agalactiae.
Streptococcus agalactiae (Group B Streptococcus; GBS) is an important pathogen and is associated with pneumonia, sepsis and meningitis in neonates and adults. GBS infections induce cytotoxicity of respiratory epithelial cells (A549) with generation of reactive oxygen species (ROS) and loss of mitochondrial membrane potential (ψm). The apoptosis of A549 cells by GBS was dependent on the activation of caspase-3 and caspase-9 with increased pro-apoptotic Bim and Bax molecules and decreased Bcl-2 pro-survival protein. Treatment of infected A549 cells with ROS inhibitors (diphenyleniodonium chloride or apocynin) prevented intracellular ROS production and apoptosis. Consequently, oxidative stress is included among the cellular events leading to apoptosis during GBS human invasive infections.
Laboratory of Molecular Biology and Physiology of Streptococci, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Andréia
A
Ferreira Eduardo da Costa
Laboratory of Biochemical and Cellular Pharmacology, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
João
J
Alfredo Moraes
Laboratory of Molecular Biology and Physiology of Streptococci, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Jessica
J
Silva Santos de Oliveira
Laboratory of Molecular Biology and Physiology of Streptococci, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Michelle
M
Hanthequeste Bittencourt Dos Santos
Laboratory of Molecular Biology and Physiology of Streptococci, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Gabriela
G
da Silva Santos
Laboratory of Biochemical and Cellular Pharmacology, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Christina
C
Barja-Fidalgo
Laboratory of Diphtheria and Corynebacteria of Clinical Relevance (LDCIC), Faculty of Medical Sciences, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Ana
A
Luiza Mattos-Guaraldi
Laboratory of Molecular Biology and Physiology of Streptococci, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Prescilla
P
Emy Nagao
D017209:Apoptosis; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013290:Streptococcal Infections / Q000378:metabolism / Q000382:microbiology* / Q000503:physiopathology*; D013292:Streptococcus agalactiae / Q000502:physiology*
D017382:Reactive Oxygen Species