Thrombin Generating Capacity and Phenotypic Association in ABO Blood Groups.
Individuals with blood group O have a higher bleeding risk than non-O blood groups. This could be explained by the lower levels of FVIII and von Willebrand Factor (VWF) levels in O individuals. We investigated the relationship between blood groups, thrombin generation (TG), prothrombin activation and thrombin inactivation. Plasma levels of VWF, FVIII, antithrombin, fibrinogen, prothrombin and α2Macroglobulin (α2M) levels were determined. TG was measured in platelet rich (PRP) and platelet poor plasma (PPP) of 217 healthy donors and prothrombin conversion and thrombin inactivation were calculated. VWF and FVIII levels were lower (75% and 78%) and α2M levels were higher (125%) in the O group. TG is 10% lower in the O group in PPP and PRP. Less prothrombin was converted in the O group (86%) and the thrombin decay capacity was lower as well. In the O group, α2M plays a significantly larger role in the inhibition of thrombin (126%). In conclusion, TG is lower in the O group due to lower prothrombin conversion, and a larger contribution of α2M to thrombin inactivation. The former is unrelated to platelet function because it is similar in PRP and PPP, but can be explained by the lower levels of FVIII.
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Romy M W
RM
Kremers
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands; Department of Pharmacology, King Abdulaziz University, Jeddah, Saudi Arabia.
Abdulrahman B O
AB
Mohamed
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands; Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Leonie
L
Pelkmans
Department of Haematology, King Abdulaziz University, Jeddah, Saudi Arabia.
Salwa
S
Hindawi
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
H Coenraad
HC
Hemker
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands; Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
H Bas
HB
de Laat
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Dana
D
Huskens
Synapse, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, the Netherlands.
Raed
R
Al Dieri
D000017:ABO Blood-Group System; D000991:Antithrombins / Q000097:blood; D015415:Biomarkers; D001777:Blood Coagulation; D001779:Blood Coagulation Factors / Q000378:metabolism; D001780:Blood Coagulation Tests; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D011516:Prothrombin / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism
D000017:ABO Blood-Group System; D000991:Antithrombins; D015415:Biomarkers; D001779:Blood Coagulation Factors; D000511:alpha-Macroglobulins; D011516:Prothrombin; D013917:Thrombin