Determination of Apparent Amylose Content in Rice by Using Paper-Based Microfluidic Chips.
Determination of apparent amylose content in rice is a key function for rice research and the rice industry. In this paper, a novel approach with paper-based microfluidic chip is reported to determine apparent amylose content in rice. The conventional color reaction between amylose and iodine was employed. Blue color of amylose-iodine complex generated on-chip was converted to gray and measured with Photoshop after the colored chip was scanned. The method for preparation of the paper chip is described. In situ generation of iodine for on-chip color reaction was designed, and factors influencing color reaction were investigated in detail. Elimination of yellow color interference of excess iodine by exploiting color removal function of Photoshop was presented. Under the optimized conditions, apparent amylose content in rice ranging from 1.5 to 26.4% can be determined, and precision was 6.3%. The analytical results obtained with the developed approach were in good agreement with those with the continuous flow analyzer method.
China National Rice Research Institute , Hangzhou 310006, China.|Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture , China.
Xianqiao
X
Hu
China National Rice Research Institute , Hangzhou 310006, China.|Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture , China.
Lin
L
Lu
China National Rice Research Institute , Hangzhou 310006, China.|Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture , China.
Changyun
C
Fang
China National Rice Research Institute , Hangzhou 310006, China.|Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture , China.
Binwu
B
Duan
China National Rice Research Institute , Hangzhou 310006, China.|Laboratory of Quality & Safety Risk Assessment for Rice (Hangzhou), Ministry of Agriculture , China.
Zhiwei
Z
Zhu
D000688:Amylose / Q000032:analysis*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D012275:Oryza / Q000737:chemistry*
D000688:Amylose
apparent amylose content; color reaction; paper-based microfluidic chips; rice