Heat shock protein 72 (Hsp72) specific induction and temporal stability in urine samples as a reliable biomarker of acute kidney injury (AKI).
We demonstrated that urinary heat shock protein of 72 KDa (Hsp72) is a sensitive biomarker for the early detection of acute kidney injury (AKI). However, whether Hsp72 induction during an AKI episode is kidney-specific is unknown, as well as, the degree of Hsp72 stability in urine samples. In rats that underwent bilateral renal ischemia and reperfusion (I/R), Hsp72 levels were evaluated in several tissues and in collected urines under different storage and temperature conditions, as well as in variable numbers of freeze-thaw cycles. The effect of room temperature and five freeze-thaw cycles on urinary Hsp72 levels was also evaluated in urine samples from AKI patients. We found that Hsp72 increased exclusively in the renal cortex of I/R group, emphasizing its performance as an AKI biomarker. Urinary-Hsp72 remained constant at room temperature (48 h), during 9 months of storage and was not affected by five freeze/thaw cycles.
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.|b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Juan Antonio
JA
Ortega-Trejo
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.|b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Rosalba
R
Pérez-Villalva
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.|b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Jonatan
J
Barrera-Chimal
b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Diego L
DL
Carrillo-Pérez
b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Luis E
LE
Morales-Buenrostro
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.|b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Gerardo
G
Gamba
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.
María Elena
ME
Flores
a Molecular Physiology Unit, Instituto De Investigaciones Biomédicas, Universidad Nacional Autónoma De México , Mexico City , Mexico and.|b Department of Nephrology , Instituto Nacional De Ciencias Médicas Y Nutrición Salvador Zubirán , Mexico City , Mexico.
Norma A
NA
Bobadilla
D058186:Acute Kidney Injury / Q000175:diagnosis* / Q000503:physiopathology / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism / Q000652:urine*; D006801:Humans; D007511:Ischemia / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D007672:Kidney Cortex / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D055550:Protein Stability; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000652:urine; D012680:Sensitivity and Specificity; D013696:Temperature
D015415:Biomarkers; D050884:HSP72 Heat-Shock Proteins
Ischemia/reperfusion; renal cortex; renal dysfunction; tubular injury