Expression of CD147 and matrix metalloproteinase-11 in colorectal cancer and their relationship to clinicopathological features.
BACKGROUND
This study aimed to investigate the expression of CD147 and MMP-11 in human colorectal cancer (CRC) and to evaluate their clinical significance.


METHODS
Real-time polymerase chain reaction was used to evaluate CD147 and MMP-11 mRNA level in 56 pairs of fresh CRC samples matched with adjacent normal mucosa. The protein expression of CD147 and MMP-11 in CRC specimens and corresponding normal colorectal mucosa were evaluated by immunohistochemistry on CRC tissue microarrays. Expression and co-localization of these two proteins in human colorectal cancer tissue were also evaluated by laser scanning confocal microscopy. Furthermore, their correlations with clinicopathological factors and overall survival after surgery were evaluated.


RESULTS
Both CD147 and MMP-11 were demonstrated to be over-expressed at mRNA level (P < 0.001, both) and protein level (P < 0.001, both) in CRC tissue than paired normal mucosa. Spearman rank test showed a positive correlation between these two proteins (P = 0.025). Immunofluorescence double staining confirmed the co-localization of CD147 and MMP-11 in paraffin-embedded tissues of CRC patients. Expression of CD147 and MMP-11 were both correlated with CRC lymph node metastasis (P = 0.021 and P = 0.031, respectively), distant metastasis (P < 0.001 and P = 0.013, respectively) and TNM stage (P = 0.006 and P = 0.049, respectively). Univariate survival analysis showed that both CD147 and MMP11 expression was significantly associated with shorter survival time (P = 0.001 and P = 0.009, respectively). Additionally, in multivariate analysis, both CD147 and MMP-11 were proved to be independent prognostic factors (P = 0.009, 0.028, respectively).


CONCLUSIONS
These results indicated that both CD147 and MMP-11 may be involved in the progression of colorectal cancer, and they are potential prognostic factors and might become new therapeutic targets for CRC patients.
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. xiuyunt@126.com.
Xiuyun
X
Tian
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. ycx198742@163.com.|Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing, People's Republic of China. ycx198742@163.com.
Chunxiang
C
Ye
Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Biochemistry and Molecular Biology, Peking University Cancer Hospital & Institute, Beijing, People's Republic of China. y3hscpku@yeah.net.
Yongyong
Y
Yang
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. lingbing1987@sohu.com.
Xiaoya
X
Guan
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. dongbin2001@163.com.
Bin
B
Dong
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. zhaomindiy@163.com.
Min
M
Zhao
Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education/Beijing, Department of Hepato-Pancreato-Biliary Surgery,  Peking University Cancer Hospital and Institute, Beijing, People's Republic of China. haochunyi@bjmu.edu.cn.
Chunyi
C
Hao
D051926:Basigin / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D053507:Matrix Metalloproteinase 11 / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics
C497886:BSG protein, human; D012333:RNA, Messenger; D051926:Basigin; D053507:Matrix Metalloproteinase 11