Spherical nanoparticle supported lipid bilayers for the structural study of membrane geometry-sensitive molecules.
Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural and topological studies of membrane geometry-sensitive molecules.
National High Magnetic Field Laboratory, Tallahassee, Florida 32310, United States.
Riqiang
R
Fu
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
Richard L
RL
Gill
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.
Edward Y
EY
Kim
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
Nicole E
NE
Briley
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
Erin R
ER
Tyndall
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
Jie
J
Xu
National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, P. R. China.
Conggang
C
Li
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, United States.
Kumaran S
KS
Ramamurthi
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
John M
JM
Flanagan
Department of Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, Pennsylvania 17033, United States.
Fang
F
Tian
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*
D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; C084307:spoVM protein, Bacillus subtilis