[Readspace solid-phase microextraction-gas chromatography for determination of 2,5-hexanedione in urine].
OBJECTIVE
To establish a method for determination of 2,5-hexanedione in urine by headspace solid-phase microextraction-gas chromatography.


METHODS
After extraction by solid-phase microextraction head, 2,5-hexanedione in urine was determined by gas chromatography and was quantified by external standard method.


RESULTS
The concentration of 2,5-hexanedione in urine showed a linear relationship within the range of 0.1-20.0 µg/ml. The regression equation was y=261.36x-1.903 3, r=0.999 2. The minimum detectable concentration was 0.01 µg/ml. The recovery rate was 92.6%-97.1%, with a relative standard deviation (RSD) of 3.3%-5.8%. The intra-day and inter-day RSDs were 3.8%-6.2% and 4.7%-6.3% respectively.


CONCLUSIONS
This determination method has no requirement for organic solvents, features simple and rapid operation, possesses higher detection sensitivity, and applies well to the determination of 2,5-hexanedione in urine.
Wei
W
Zou
Mengping
M
Zhang
Haiyan
H
Wei
D002849:Chromatography, Gas; D006588:Hexanones / Q000652:urine*; D006801:Humans; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction
D006588:Hexanones; C011269:2,5-hexanedione