Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast.
Understanding how genes are regulated, decoding their "regulome", is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.
Department of Biology, Memorial University of Newfoundland, St. John's, NL, Canada, A1B 3X5.|Department of Computer Science, Memorial University of Newfoundland, St. John's, NL, Canada.
Lourdes
L
Peña-Castillo
Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 3525, Paris, 75724, France. gbreard@pasteur.fr.
Gwenael
G
Badis
D001665:Binding Sites; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA
Binding sites; DNA binding domain; Enhancers; Transcription factors; Transcription regulation; cis-regulatory element