Lack of tissue accumulation of grape seed flavanols after daily long-term administration in healthy and cafeteria-diet obese rats.
After ingestion flavanols are metabolized by phase-II enzymes and the microbiota and are distributed throughout the body depending on several factors. Herein we aim to evaluate whether flavanols are tissue-accumulated after the long-term administration of a grape seed polyphenol extract (GSPE) in rats and to study if compounds present in tissues differ in a cafeteria-diet obesity state. For that, plasma, liver, mesenteric white adipose tissue (MWAT), brain, and aorta flavanol metabolites from standard chow-diet-fed (ST) and cafeteria-diet-fed (CAF) rats were analyzed by high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) 21 h after the last 12-week-daily GSPE (100 mg/kg) dosage. Results showed that long-term GSPE intake did not trigger a flavanol tissue accumulation, indicating a clearance of products at each daily dosage. Therefore, results suggest that polyphenol benefits in a disease state would be due to a daily pulsatile effect. Moreover, obesity induced by diet also influences the metabolism and bioavailability of flavanols in rats.
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.
Maria
M
Margalef
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.
Zara
Z
Pons
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.
Lisard
L
Iglesias-Carres
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.
Francisca Isabel
FI
Bravo
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.|Technological Center of Nutrition and Health (CTNS), TECNIO, CEICS , Reus 43204, Spain.
Begoña
B
Muguerza
Nutrigenomic Research Group, Biochemistry and Biotechnology Department, Rovira i Virgili University , Tarragona 43003, Spain.
Anna
A
Arola-Arnal
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D056604:Grape Seed Extract / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000378:metabolism*; D059808:Polyphenols / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000378:metabolism; D027843:Vitis / Q000378:metabolism*
D056604:Grape Seed Extract; D059808:Polyphenols
bioavailability; flavonoid-type polyphenols; metabolites; obesity; rats; tissue distribution