Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae.
The biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. This study aimed at evaluating the predatory activity of fungal isolates of Duddingtonia flagrans and Clonostachys rosea species and its association on infective larvae (L3) of H. contortus in microplots formed by grasses and maintained in a protected environment. All groups were added with 10 mL of an aqueous suspension with 618 H. contortus L3 approximately. Group 1 was used as control and only received the infective larvae. Groups 2 and 3 received D. flagrans chlamydospores and C. rosea conidia at doses of 5 × 10(6). Group 4 received the combination of 5 × 10(6) D. flagrans chlamydospores + 5 × 10(6) C. rosea conidia. D. flagrans and C. rosea showed nematicidal effectiveness reducing by 91.5 and 88.9%, respectively, the population of H. contortus L3. However, when used in combination efficiency decreased to 74.5% predation of H. contortus L3. These results demonstrate the need for further studies to determine the existence of additive effects, synergistic or antagonistic, between these species.
Departamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, Brazil ; URECO/EPAMIG, 35650000 Pitangui, MG, Brazil.
Manoel Eduardo
ME
da Silva
Universidade Vila Velha, Vila Velha, ES, Brazil.
Fabio Ribeiro
FR
Braga
Área de Helmintologia, CENID-Parasitologia, INIFAP, Jiutepec, MOR, Mexico.
Pedro Mendoza
PM
de Gives
Área de Helmintologia, CENID-Parasitologia, INIFAP, Jiutepec, MOR, Mexico.
Miguel Angel Mercado
MA
Uriostegui
Departamento de Microbiología Ambiental y Biotecnología, UAC, CAM, Mexico.
Manuela
M
Reyes
Programa de Pós Graduação em Ciências Farmacêuticas (PPGF-UVV), Universidade Vila Velha, 29102920 Vila Velha, ES, Brazil.
Filippe Elias de Freitas
FE
Soares
Departamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, Brazil.
Lorendane Millena
LM
de Carvalho
Programa de Pós Graduação em Ciências Farmacêuticas (PPGF-UVV), Universidade Vila Velha, 29102920 Vila Velha, ES, Brazil.
Francielle Bosi
FB
Rodrigues
Departamento de Veterinária, Universidade Federal de Viçosa, 36570000 Viçosa, MG, Brazil.
Jackson Victor
JV
de Araújo
D000818:Animals; D059586:Duddingtonia / Q000502:physiology*; D006188:Haemonchiasis / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary; D006189:Haemonchus / Q000472:pathogenicity*; D006999:Hypocreales / Q000502:physiology*; D007814:Larva / Q000472:pathogenicity; D010572:Pest Control, Biological / Q000379:methods*; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology / Q000517:prevention & control*; D034561:Sheep, Domestic