One-to-one encapsulation based on alternating droplet generation.
This paper reports the preparation of encapsulated particles as models of cells using an alternating droplet generation encapsulation method in which the number of particles in a droplet is controlled by a microchannel to achieve one-to-one encapsulation. Using a microchannel in which wettability is treated locally, the fluorescent particles used as models of cells were successfully encapsulated in uniform water-in-oil-in-water (W/O/W) emulsion droplets. Furthermore, 20% of the particle-containing droplets contained one particle. Additionally, when a surfactant with the appropriate properties was used, the fluorescent particles within each inner aqueous droplet were enclosed in the merged droplet by spontaneous droplet coalescence. This one-to-one encapsulation method based on alternating droplet generation could be used for a variety of applications, such as high-throughput single-cell assays, gene transfection into cells or one-to-one cell fusion.
Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8563, Japan.
Hirotada
H
Hirama
Department of Human and Engineered Environmental Studies, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8563, Japan.
Toru
T
Torii
D002459:Cell Fusion; D004655:Emulsions / Q000737:chemistry*; D066292:Lipid Droplets / Q000737:chemistry*; D059010:Single-Cell Analysis; D014867:Water / Q000737:chemistry*; D017155:Wettability
D004655:Emulsions; D014867:Water