Impact of silk fibroin-based scaffold structures on human osteoblast MG63 cell attachment and proliferation.
The present study was carried out to investigate the impact of various types of silk fibroin (SF) scaffolds on human osteoblast-like cell (MG63) attachment and proliferation. SF was isolated from Bombyx mori silk worm cocoons after degumming. Protein concentration in the degummed SF solution was estimated using Bradford method. Aqueous SF solution was used to fabricate three different types of scaffolds, viz, electrospun nanofiber mat, sponge, and porous film. The structures of the prepared scaffolds were characterized using optical microscopy and field emission scanning electron microscopy. The changes in the secondary structure of the proteins and the thermal behavior of the scaffolds were determined by Fourier transform infrared spectroscopy and thermo-gravimetric analysis, respectively. The biodegradation rate of scaffolds was determined by incubating the scaffolds in simulated body fluid for 4 weeks. MG63 cells were seeded on the scaffolds and their attachment and proliferation onto the scaffolds were studied. The MTT assay was carried out to deduce the toxicity of the developed scaffolds. All the scaffolds were found to be biocompatible. The amount of collagen produced by the osteoblast-like cells growing on different scaffolds was estimated.
Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India ; Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Aneesia
A
Varkey
Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Elakkiya
E
Venugopal
Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Ponjanani
P
Sugumaran
Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Gopinathan
G
Janarthanan
Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Mamatha M
MM
Pillai
Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Selvakumar
S
Rajendran
Advanced Textile and Polymer Research Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India.
Amitava
A
Bhattacharyya
D000818:Animals; D012831:Bombyx; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D005349:Fibroins / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D054457:Tissue Scaffolds / Q000737:chemistry*
D003094:Collagen; D005349:Fibroins
electrospun nanofiber; osteoblast; porous film; silk fibroin scaffold; sponge