Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration.
Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'SHG ) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.
Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Richard
R
Cisek
Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Danielle
D
Tokarz
Department of Plant Physiology, Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Strasse 24-25 Building 20, 14476 Potsdam, Germany ; Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Martin
M
Steup
Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Ian J
IJ
Tetlow
Department of Molecular and Cellular Biology, College of Biological Science, Summerlee Science Complex, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada.
Michael J
MJ
Emes
Department of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark.
Kim H
KH
Hebelstrup
Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Andreas
A
Blennow
Department of Chemical and Physical Sciences, Department of Physics and Institute for Optical Sciences, University of Toronto, 3359 Mississauga Road, Mississauga, ON, L5L 1C6, Canada.
Virginijus
V
Barzda
(170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine