Amniotic Mesenchymal Stem Cells Can Enhance Angiogenic Capacity via MMPs In Vitro and In Vivo.
The aim of this study was to evaluate the angiogenic capacity and proteolytic mechanism of coculture using human amniotic mesenchymal stem cells (hAMSCs) with human umbilical vein endothelial cells (HUVECs) in vivo and in vitro by comparing to those of coculture using bone marrow mesenchymal stem cells with HUVEC. For the in vivo experiment, cells (HUVEC-monoculture, HUVEC-hAMSC coculture, and HUVEC-BMMSC coculture) were seeded in fibrin gels and injected subcutaneously in nude mice. The samples were collected on days 7 and 14 and histologically analyzed by H&E and CD31 staining. CD31-positive staining percentage and vessel-like structure (VLS) density were evaluated as quantitative parameters for angiogenesis. The increases of CD31-positive staining area and VLS density in both HUVEC-hAMSC group and HUVEC-BMMSC group were found between two time points, while obvious decline of those was observed in HUVEC-only group. For the in vitro experiment, we utilized the same 3D culture model to investigate the proteolytic mechanism related to capillary formation. Intensive vascular networks formed by HUVECs were associated with hAMSCs or BMMSCs and related to MMP2 and MMP9. In conclusion, hAMSCs shared similar capacity and proteolytic mechanism with BMMSCs on neovascularization.
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China ; Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China.
Fei
F
Jiang
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China ; Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China.
Jie
J
Ma
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China ; Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China.
Yi
Y
Liang
Department of Stomatology, Taihe Hospital, Hubei University of Medicine, No. 32, Renmingnan Road, Shiyan, Hubei 442000, China.
Yuming
Y
Niu
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China ; Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China.
Ning
N
Chen
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China ; Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing Medical University, No. 140, Han Zhong Road, Nanjing, Jiangsu 210029, China.
Ming
M
Shen
D000650:Amnion / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D018920:Coculture Techniques; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D011247:Pregnancy
C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9