Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study.
We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.
Premedical Science, College of Medicine, Chosun University, Gwangju, South Korea.
Choon-Mee
CM
Kim
Department of Internal Medicine, School of Medicine, Chosun University, Gwangju, South Korea.
Min Keun
MK
Cho
Department of Internal Medicine, School of Medicine, Chosun University, Gwangju, South Korea drongkim@chosun.ac.kr.
Dong-Min
DM
Kim
Department of Internal Medicine, School of Medicine, Chosun University, Gwangju, South Korea.
Na-Ra
NR
Yun
Department of Neurosurgery, School of Medicine, Chosun University, Gwangju, South Korea.
Seok Won
SW
Kim
Department of Laboratory Medicine, School of Medicine, Chosun University, Gwangju, South Korea.
Sook Jin
SJ
Jang
Department of Medical Education, School of Medicine, Chosun University, Gwangju, South Korea.
Young-Joon
YJ
Ahn
Department of Urology, School of Medicine, Chosun University, Gwangju, South Korea.
Donghoon
D
Lim
D000328:Adult; D017931:DNA Primers / Q000235:genetics*; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012285:Orientia tsutsugamushi / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012189:Retrospective Studies; D012612:Scrub Typhus / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S