Comprehensive Screen of Metal Oxide Nanoparticles for DNA Adsorption, Fluorescence Quenching, and Anion Discrimination.
Although DNA has been quite successful in metal cation detection, anion detectioin remains challenging because of the charge repulsion. Metal oxides represent a very important class of materials, and different oxides might interact with anions differently. In this work, a comprehensive screen of common metal oxide nanoparticles (MONPs) was carried out for their ability to adsorb DNA, quench fluorescence, and release adsorbed DNA in the presence of target anions. A total of 19 MONPs were studied, including Al2O3, CeO2, CoO, Co3O4, Cr2O3, Fe2O3, Fe3O4, In2O3, ITO, Mn2O3, NiO, SiO2, SnO2, a-TiO2 (anatase), r-TiO2 (rutile), WO3, Y2O3, ZnO, ZrO2. These MONPs have different DNA adsorption affinity. Some adsorb DNA without quenching the fluorescence, while others strongly quench adsorbed fluorophores. They also display different affinity toward anions probed by DNA desorption. Finally, CeO2, Fe3O4, and ZnO were used to form a sensor array to discriminate phosphate, arsenate, and arsenite from the rest using linear discriminant analysis. This study not only provides a solution for anion discrimination using DNA as a signaling molecule but also provides insights into the interface of metal oxides and DNA.
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
Biwu
B
Liu
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
Juewen
J
Liu
D000327:Adsorption; D000838:Anions / Q000737:chemistry*; D001149:Arsenates / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002563:Cerium / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D016002:Discriminant Analysis; D052203:Ferrosoferric Oxide / Q000737:chemistry; D057446:Hydrodynamics; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D012542:Scattering, Radiation; D017640:Silicates / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D015034:Zinc Oxide / Q000737:chemistry
D000838:Anions; D001149:Arsenates; D010087:Oxides; D010710:Phosphates; D017640:Silicates; D002563:Cerium; C030583:ceric oxide; D004247:DNA; D015034:Zinc Oxide; D052203:Ferrosoferric Oxide
DNA; arsenate; biosensors; metal oxides; phosphate