[Antimicrobial effect on some zoonotic bacteria, of the cell-free fermentation fluid and purified peptide fraction of the entomopathogenic bacterium, Xenorhabdus budapestensis].
BACKGROUND
Many multi-resistant patogens appear continuously resulting in a permanent need for the development of novel antibiotics. A large number of antibiotics introduced in clinical and veterinary practices are not effective. Antibacterial peptides with unusual mode of action may represent a promising option against multi-resistant pathogens. The entomopathogenic Xenorhabdus budapestensis bacteria produce several different antimicrobial peptides compounds such as bicornutin-A and fabclavin.


OBJECTIVE
The aim of the authors was to evaluate the in vitro antibacterial effect of Xenorhabdus budapestensis using zoonotic patogen bacteria.


METHODS
Cell-free conditioned media and purified peptide fractions of Xenorhabdus budapestensis were tested on Gram-positive (Rhodococcus equi, Erysipelothrix rhusiopathia, Staphylococcus aureus, Streptococcus equi, Corynebacterium pseudotuberculosis, Listeria monocytagenes) and Gram-negative bacteria (Salmonella gallinarum, Salmonella derbi, Bordatella bronchoseptica, Escherichia coli, Pasteurella multocida, Aeromonas hydrophila) using agar diffusion test on blood agar plates.


RESULTS
It was found that Xenorhabdus budapestensis bacteria produced compounds with strong and dose-dependent effects on the tested organisms. Purified peptid fraction exerted a more marked effect than cell free conditioned media. Gram-positive bacteria were more sensitive to this antibacterial effect than Gram-negative bacteria.


CONCLUSIONS
Antibacterial peptide compound from Xenorhabdus budapestensis exert marked antibacterial effect on zoonotic patogen bacteria and they should be further evaluated in future for their potential use in the control or prevention of zoonoses.
Epidemiológia Tanszék, Semmelweis Egyetem, Egészségtudományi Kar Budapest.
Erzsébet
E
Burgettiné Böszörményi
Epidemiológia Tanszék, Semmelweis Egyetem, Egészségtudományi Kar Budapest.
István
I
Barcs
Epidemiológia Tanszék, Semmelweis Egyetem, Egészségtudományi Kar Budapest.
Gyula
G
Domján
Mérnöki Kar, Pannon Egyetem Veszprém.
Katalin
K
Bélafiné Bakó
Department of Bacteriology, University of Wisconsin Madison, USA.
András
A
Fodor
Járványtani Mikrobiológiai Tanszék, Szent István Egyetem, Állatorvos-tudományi Kar Budapest.
László
L
Makrai
Mérnöki Kar, Pannon Egyetem Veszprém.
Dávid
D
Vozik
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002474:Cell-Free System; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D010455:Peptides / Q000494:pharmacology; D020593:Xenorhabdus; D015047:Zoonoses / Q000188:drug therapy / Q000382:microbiology*
D000890:Anti-Infective Agents; D010455:Peptides
Xenorhabdus budapestensis; antimicrobial activity; antimikrobiális hatás; bicornutin-A; fabclavin; zoonosis; zoonózis