Histopathological and biomechanical evaluation of bone healing properties of DBM and DBM-G90 in a rabbit model.
OBJECTIVE
The present study was designed to investigate the effects of DBM and DBM-G90 on bone healing in a rabbit model.


METHODS
Thirty male white albino rabbits were used in this study. An incision was made in all rabbits under general anesthesia directly over the radius in order to expose it. A 10-mm segmental defect was created on the middle portion of each radius. The defects of 10 rabbits (Group I) were filled with DBM Block and Strip (Zimmer, Inc., Warsaw, IN, USA), the defects of 10 rabbits (Group II) were filled with DBM soaked in G90, and the defects of 10 rabbits (Group III/control) were left empty. The rabbits were euthanized at 60 days postoperatively for histopathological and biomechanical evaluation.


RESULTS
At the histopathologic level, the defects of the animals in the DBM and DBM-G90 groups showed more advanced healing criteria than those of the control group. In biomechanical findings, there was a statistically significant difference between the injured bones and contralateral normal bones of the control group in terms of measured strength. There was not a statistically significant difference between the treated bones of the DBM and DBM-G90 groups with contralateral normal bones, nor was there a statistically significant difference between the treated bones of the DBM and DBM-G90 groups with the treated bones of the control group, in terms of other biomechanical tests.


CONCLUSIONS
Based on the histopathological and biomechanical findings, the DBM and DBM-G90 groups demonstrated superior osteogenic potential; however, G90 shows no superiority over DBM on bone healing.
Shiraz University School of Veterinary Medicine, Department of Veterinary Surgery and Radiolog, Shiraz, Iran.
Abdolhamid
A
Meimandi Parizi
Shiraz University School of Veterinary Medicine, Department of Veterinary Pathobiology, Shiraz University, Shiraz, Iran.
Ahmad
A
Oryan
Shiraz University School of Veterinary Medicine, Department of Veterinary Surgery and Radiolog, Shiraz, Iran.
Shahram
S
Haddadi
Shahrekord University Faculty of Veterinary Medicine, Department of Veterinary Surgery and Radiology, Shahrekord, Iran.
Amin
A
Bigham Sadegh
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000008:administration & dosage*; D001842:Bone and Bones / Q000002:abnormalities* / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D011817:Rabbits; D014945:Wound Healing
D001672:Biocompatible Materials; D018786:Bone Substitutes