Brucella abortus Cell Cycle and Infection Are Coordinated.
Brucellae are facultative intracellular pathogens. The recent development of methods and genetically engineered strains allowed the description of cell-cycle progression of Brucella abortus, including unipolar growth and the ordered initiation of chromosomal replication. B. abortus cell-cycle progression is coordinated with intracellular trafficking in the endosomal compartments. Bacteria are first blocked at the G1 stage, growth and chromosome replication being resumed shortly before reaching the intracellular proliferation compartment. The control mechanisms of cell cycle are similar to those reported for the bacterium Caulobacter crescentus, and they are crucial for survival in the host cell. The development of single-cell analyses could also be applied to other bacterial pathogens to investigate their cell-cycle progression during infection.
University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium. Electronic address: xavier.debolle@unamur.be.
Xavier
X
De Bolle
University of Chicago, Gordon Center for Integrative Science W125, 929 E. 57th Street, Chicago, IL 60637, USA.
Sean
S
Crosson
University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium.
Jean-Yves
JY
Matroule
University of Namur, 61 rue de Bruxelles, 5000 Namur, Belgium.
Jean-Jacques
JJ
Letesson
D002003:Brucella abortus / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism* / Q000502:physiology; D002006:Brucellosis / Q000235:genetics / Q000473:pathology; D016935:Caulobacter crescentus / Q000235:genetics / Q000472:pathogenicity; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line / Q000382:microbiology; D049109:Cell Proliferation; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000502:physiology; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D011992:Endosomes / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints / Q000235:genetics / Q000502:physiology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D006801:Humans; D053858:Metabolic Networks and Pathways; D021381:Protein Transport
D004269:DNA, Bacterial
Brucella; cell cycle; growth; intracellular infection; replication