Single- versus Multiple-Pest Infestation Affects Differently the Biochemistry of Tomato (Solanum lycopersicum 'Ailsa Craig').
Tomato is susceptible to pest infestations by both spider mites and aphids. The effects of each individual pest on plants are known, whereas multiple-pest infestations have received little interest. We studied the effects of single- versus multiple-pest infestation by Tetranychus urticae and Myzus persicae on tomato biochemistry (Solanum lycopersicum) by combining a metabolomic approach and analyses of carotenoids using UHPLC-ToF-MS and volatiles using GC-MS. Plants responded differently to aphids and mites after 3 weeks of infestation, and a multiple infestation induced a specific metabolite composition in plants. In addition, we showed that volatiles emissions differed between the adaxial and abaxial leaf epidermes and identified compounds emitted particularly in response to a multiple infestation (cyclohexadecane, dodecane, aromadendrene, and β-elemene). Finally, the carotenoid concentrations in leaves and stems were more affected by multiple than single infestations. Our study highlights and discusses the interplay of biotic stressors within the terpenoid metabolism.
Leibniz Institute of Vegetable and Ornamental Crops (IGZ) , Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.|Institute of Nutritional Science, University of Potsdam , Arthur Scheunert-Allee 114-116, 14558 Nuthethal, Germany.
Audrey
A
Errard
Faculty of Life Sciences, Urban Plant Ecophysiology, Humboldt-Universität zu Berlin , Lentzeallee 55/57, 14195 Berlin, Germany.
Christian
C
Ulrichs
Institute for Strategies and Technology Assessment, Julius Kühn-Institut (JKI) , Stahnsdorfer Damm 81, 14532 Kleinmachnow, Germany.
Stefan
S
Kühne
Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Julius Kühn-Institut (JKI) , Königin-Luise-Strasse 19, 14195 Berlin, Germany.
Inga
I
Mewis
Leibniz Institute of Vegetable and Ornamental Crops (IGZ) , Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
Mario
M
Drungowski
Leibniz Institute of Vegetable and Ornamental Crops (IGZ) , Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
Monika
M
Schreiner
Leibniz Institute of Vegetable and Ornamental Crops (IGZ) , Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.|Institute of Nutritional Science, University of Potsdam , Arthur Scheunert-Allee 114-116, 14558 Nuthethal, Germany.
Susanne
S
Baldermann
D000818:Animals; D001042:Aphids; D002338:Carotenoids / Q000032:analysis; D018551:Solanum lycopersicum / Q000737:chemistry / Q000378:metabolism*; D055432:Metabolomics; D010935:Plant Diseases; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D013729:Terpenes / Q000378:metabolism; D040102:Tetranychidae; D055549:Volatile Organic Compounds / Q000032:analysis
D013729:Terpenes; D055549:Volatile Organic Compounds; D002338:Carotenoids
Solanaceae; Solanum lycopersicum; aphids; carotenoids; plant volatiles; spider mites; systemic response; terpenoids