Anti-inflammatory and anti-arthritic effects of pentacyclic triterpenoids maslinic acid through NF-κB inactivation.
METHODS
Consumption of olives (Olea europaea L.), including table olives and oil, is associated with low incidence of inflammation-related diseases. In this study, the effects of maslinic acid (MA), the main constituent of olive pomace, on the expression of genes and proteins involved in inflammatory activity in RAW 264.7 cells were investigated. Furthermore, the effect of MA on carrageenan-induced paw edema and collagen antibody induced arthritis in mice was determined.


RESULTS
We confirmed the suppressive effects of MA on LPS-induced tumor necrosis factor α production and on the expression of inflammatory response associated genes in RAW 264.7 cells. We also clarified the suppressive effect of MA on LPS-induced nuclear factor-kappa B activation as well as the phosphorylation of IκB-α. Furthermore, MA (200 mg/kg in the edema model or 100 mg/kg in the arthritis model) exerted anti-inflammatory and antiarthritis effects as shown by the suppression of paw edema, arthritis score, inflammatory cells, and destruction of synovium in knee joints.


CONCLUSIONS
Olive products containing MA are useful as a new preventive and therapeutic food ingredient for inflammatory and arthritic diseases.
Nippon Flour Mills Co, Ltd, Central Laboratory, Midorigaoka, Atsugi, Kanagawa, Japan.
Satoshi
S
Fukumitsu
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.|Alliance for Research on North Africa (ARENA), University of Tsukuba, Tsukuba, Ibaraki, Japan.
Myra O
MO
Villareal
Toyo Olive Co. Ltd, Shodoshima-cho, Shozu-gun, Kagawa, Japan.
Takashi
T
Fujitsuka
Nippon Flour Mills Co, Ltd, Central Laboratory, Midorigaoka, Atsugi, Kanagawa, Japan.
Kazuhiko
K
Aida
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.|Alliance for Research on North Africa (ARENA), University of Tsukuba, Tsukuba, Ibaraki, Japan.
Hiroko
H
Isoda
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D018501:Antirheumatic Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy; D002351:Carrageenan / Q000633:toxicity; D002460:Cell Line; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D031658:Olea / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D014315:Triterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018501:Antirheumatic Agents; D002351:Carrageenan; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; C412811:maslinic acid; D009828:Oleanolic Acid
Arthritis; Inflammation; Locomotive syndrome; Maslinic acid; Olive fruit