Sensitive and rapid detection of endogenous hydrogen sulﬁde distributing in different mouse viscera via a two-photon fluorescent probe.
Development of efficient methods for detection of endogenous H2S in living cells and tissues is of considerable signiﬁcance for better understanding the biological and pathological functions of H2S. Two-photon (TP) fluorescent probes are favorable as powerful molecular tools for studying physiological process due to its non-invasiveness, high spatiotemporal resolution and deep-tissues imaging. Up to date, several TP probes for intracellular H2S imaging have been designed, but real-time imaging of endogenous H2S-related biological processes in tissues is hampered due to low sensitivity, long response time and interference from other biothiols. To address this issue, we herein report a novel two-photon fluorescent probe (TPP-H2S) for highly sensitive and fast monitoring and imaging H2S levels in living cells and tissues. In the presence of H2S, it exhibits obviously improved sensitivity (LOD: 0.12 μM) and fast response time (about 2 min) compared with the reported two-photon H2S probes. With two-photon excitation, TPP-H2S displays high signal-to-noise ratio and sensitivity even no interference in cell growth media. As further application, TPP-H2S is applied for fast imaging of H2S in living cells and different fresh tissues by two-photon confocal microscope. Most importantly we first measured the endogenous H2S level in different viscera by vivisection and found that the distribution of endogenous H2S mostly in brain, liver and lung. The excellent sensing properties of TPP-H2S make it a practically useful tool for further studying biological roles of H2S.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Qian
Q
Chen
Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, 410013, China.
Jinfeng
J
Yang
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Electronic address: yinhuili16@163.com.
Yinhui
Y
Li
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Jing
J
Zheng
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China; School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, China. Electronic address: yangrh@pku.edu.cn.
Ronghua
R
Yang
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D051379:Mice; D015394:Molecular Structure; D017785:Photons; D013329:Structure-Activity Relationship; D014781:Viscera / Q000737:chemistry*
D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide
Hydrogen sulﬁde; Imaging; Two-photon probe; Viscera