Capillary zone electrophoresis for bottom-up analysis of complex proteomes.
Capillary zone electrophoresis (CZE) is emerging as a useful tool in proteomic analysis. Interest arises from dramatic improvements in performance that result from improvements in the background electrolyte used for the separation, the incorporation of advanced sample injection methods, the development of robust and sensitive electrospray interfaces, and the coupling with Orbitrap mass spectrometers with high resolution and sensitivity. The combination of these technologies produces performance that is rapidly approaching the performance of UPLC-based methods for microgram samples and exceeds the performance of UPLC-based methods for mid- to low nanogram samples. These systems now produce over 10 000 peptide IDs in a single 100-min analysis of the HeLa proteome.
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Liangliang
L
Sun
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Guijie
G
Zhu
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Xiaojing
X
Yan
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Zhenbin
Z
Zhang
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Roza
R
Wojcik
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Matthew M
MM
Champion
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.
Norman J
NJ
Dovichi
D019075:Electrophoresis, Capillary / Q000592:standards; D006801:Humans; D020543:Proteome / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods; D058996:Quality Improvement; D021241:Spectrometry, Mass, Electrospray Ionization
D020543:Proteome
Bottom-up proteomics; Capillary electrophoresis; Cell biology; Electrokinetically pumped nanospray; Technology