Effects of bisphenol A on lipid metabolism in rare minnow Gobiocypris rarus.
As one of the most abundant endocrine disrupting compounds (EDCs), bisphenol A (BPA) exists ubiquitously in an aquatic environment. Many studies on fish have focused on the reproductive toxicity effects of BPA. However, few has involved the effects of BPA on lipid metabolism. To evaluate the effects of BPA on lipid metabolism, we determine the hepato-somatic index, triglyceride contents in the liver and serum, the activities of acetyl-CoA carboxylase (ACC), fatty acid synthase (FASN), carnitine palmitoyltransferase (CPT1), and glycerol-3-phosphate acyltransferase (GPAT) enzymes and the mRNA expression of acaca, acacb, fasn, gpat1 and cpt1α in Gobiocypris rarus after exposure to BPA for 28days. BPA induced increasing tendency of triglyceride contents in male fish, possibly due to up-regulated lipid synthesis. Although in this process, fatty acid β-oxidation was up-regulated, it might be compensated by increasing lipogenesis. Our result also revealed that the GPAT enzyme might play a key role in lipid metabolism disturbance by BPA in females. Besides, the effect of BPA on the fatty acid β-oxidation pathway might be gender-dependent in G. rarus. Further studies are needed to investigate BPA's effects on the signaling pathway of lipid metabolism.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Yongjing
Y
Guan
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Jiancao
J
Gao
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Yingying
Y
Zhang
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Shu
S
Chen
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
Cong
C
Yuan
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: zzwang@nwsuaf.edu.cn.
Zaizhao
Z
Wang
D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D003530:Cyprinidae; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D050356:Lipid Metabolism / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000633:toxicity*; D014280:Triglycerides / Q000819:agonists / Q000097:blood; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D014280:Triglycerides; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Gobiocypris rarus; Lipid metabolism; Triglycerides