Improvement in Long-Term Memory following Chronic Administration of Eryngium planum Root Extract in Scopolamine Model: Behavioral and Molecular Study.
Eryngium planum L. (EP) is as a rare medicinal plant with a lot of potentials as pharmaceutical crops. The aim of our study was to assess the effect of subchronic (28-fold) administration of a 70% ethanol extract of EP roots (200 mg/kg, p.o.) on behavioral and cognitive responses in Wistar rats linked with acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and beta-secretase (BACE-1) mRNA levels and AChE and BuChE activities in the hippocampus and frontal cortex. On the last day of experiment, 30 min after the last dose of EP or Huperzine A (HU), scopolamine (SC) was given at a dose of 0.5 mg/kg b.w. intraperitoneally. The results of a passive avoidance test showed an improvement in long-term memory produced by the EP extract in both scopolamine-induced rats and control group. EP caused an insignificant inhibition of AChE and BuChE activities in the frontal cortex and the hippocampus. EP decreased mRNA AChE, BuChE, and BACE-1 levels, especially in the cortex. Our results suggest that the EP extract led to the improvement of the long-term memory in rats coupled with total saponin content. The mechanism of EP action is probably complicated, since HPLC-MS analysis showed 64 chemical compounds (phenolics, saponins) in the extract of EP roots.
Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland ; Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland.
Marcin
M
Ozarowski
Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland.
Barbara
B
Thiem
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland ; Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland.
Przemyslaw L
PL
Mikolajczak
Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland.
Anna
A
Piasecka
Department of Pathogen Genetics and Plant Resistance, Metabolomics Team, Institute of Plant Genetics of the Polish Academy of Science, Strzeszynska 34, 60-479 Poznan, Poland.
Piotr
P
Kachlicki
Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland.
Michal
M
Szulc
Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland.
Ewa
E
Kaminska
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland.
Anna
A
Bogacz
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland.
Radoslaw
R
Kujawski
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland ; Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland.
Joanna
J
Bartkowiak-Wieczorek
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland.
Malgorzata
M
Kujawska
Department of Toxicology, Poznan University of Medical Sciences, Dojazd 30, 60-631 Poznan, Poland.
Jadwiga
J
Jodynis-Liebert
Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland.
Jaromir
J
Budzianowski
Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, 61-861 Poznan, Poland.
Izabela
I
Kędziora
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland ; Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland ; Laboratory of Molecular Biology, Poznan University of Medical Sciences, Polna 33, 60-535 Poznan, Poland.
Agnieszka
A
Seremak-Mrozikiewicz
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland ; Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland ; Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland.
Boguslaw
B
Czerny
Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland.
Teresa
T
Bobkiewicz-Kozłowska