Comparative Proteomic Analysis of Gossypium thurberi in Response to Verticillium dahliae Inoculation.
Verticillium wilt is threatening cotton productivity globally. This disease is caused by soil-borne Verticillium dahliae which directly infects cotton roots, and exclusively colonizes and occludes xylem vessels, finally resulting in necrosis, defoliation, and most severely, plant death. For the first time, iTRAQ (isobaric tags for relative and absolute quantification) was applied to screen the differentially expressed proteins of Gossypium thurberi inoculated with V. dahliae. A total of 6533 proteins were identified from the roots of G. thurberi after inoculation with V. dahliae, and 396 showed up- and 279 down-regulated in comparison to a mock-inoculated roots. Of these identified proteins, the main functional groups were those involved in cell wall organization and reinforcement, disease-resistant chemicals of secondary metabolism, phytohormone signaling, pathogenesis-related proteins, and disease-resistant proteins. Physiological and biochemical analysis showed that peroxidase activity, which promotes the biosynthesis and accumulation of lignin, was induced early in the hypocotyl after inoculation with V. dahliae. Similarly, salicylic acid also accumulated significantly in hypocotyl of the seedlings after inoculation. These findings provide an important knowledge of the molecular events and regulatory networks occurring during G. thurberi-V. dahliae interaction, which may provide a foundation for breeding disease-resistance in cotton.
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. fangweiping0425@sina.com.
Weiping
W
Fang
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. xiedeyi101@sina.com.
Deyi
D
Xie
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Heqinanyang@sina.com.
Heqin
H
Zhu
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. cotton169@sina.com.
Wu
W
Li
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. lele20032@sina.com.
Zhenzhen
Z
Xu
Plant Protection Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. yangzi0224_sohu@sina.com.
Lirong
L
Yang
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. lizhifang2015@gmail.com.
Zhifang
Z
Li
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA. li.sun@ttu.edu.
Li
L
Sun
Department of Crop Biotechnology, Agronomy College, Henan Agricultural University, Zhengzhou 450002, China. wjx29287@sina.com.
Jinxia
J
Wang
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. nlh200999@sina.com.
Lihong
L
Nie
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. tangzhongjie2001@sina.com.
Zhongjie
Z
Tang
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. lvshuping316@sina.com.
Shuping
S
Lv
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. fazcotton@sina.com.
Fu'an
F
Zhao
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. yangming1697@sina.com.
Yao
Y
Sun
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. zzzym5@sina.com.
Yuanming
Y
Zhao
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. hjnwikiscott@gmail.com.
Jianan
J
Hou
Economic Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. yyxxjj7910@sina.com.
Xiaojie
X
Yang
D002473:Cell Wall / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics*; D005419:Flavonoids / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D003368:Gossypium / Q000378:metabolism / Q000382:microbiology*; D008031:Lignin / Q000096:biosynthesis; D009336:Necrosis / Q000382:microbiology; D009195:Peroxidase / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology; D040901:Proteomics; D020156:Salicylic Acid / Q000378:metabolism; D012988:Soil Microbiology; D020172:Verticillium / Q000472:pathogenicity*; D052584:Xylem / Q000382:microbiology
D005419:Flavonoids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D010649:Phenylalanine; D008031:Lignin; D009195:Peroxidase; D020156:Salicylic Acid
cotton; defense response; fungus; iTRAQ