IS200 and multilocus sequence typing for the identification of Salmonella enterica serovar Typhi strains from Indonesia.
In this work, IS200 and multi-locus sequence typing (MLST) were used to analyze 19 strains previously serotyped as Salmonella enterica serovar Typhi and isolated in Indonesia (16 strains), Mexico (2 strains), and Switzerland (1 strain). Most of the strains showed the most common Typhi sequence types, ST1 and ST2, and a new Typhi genotype (ST1856) was described. However, one isolate from Mexico and another from Indonesia were of the ST365 and ST426 sequence types, indicating that they belonged to serovars Weltevreden and Aberdeen, respectively. These results were supported by the amplification of IS200 fragments, which rapidly distinguish Typhi from other serovars. Our results demonstrate the utility of IS200 and MLST in the classification of Salmonella strains into serovars. These methods provide information on the clonal relatedness of strains isolated worldwide.
National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
Areli
A
Martínez-Gamboa
Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos, Mexico.
Claudia
C
Silva
Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos, Mexico.
Marcos
M
Fernández-Mora
Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos, Mexico.
Magdalena
M
Wiesner
National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico.
Alfredo
A
Ponce de León
Institute of Biotechnology, Department of Molecular Microbiology, UNAM, Cuernavaca, Morelos, Mexico.
Edmundo
E
Calva
D001483:Base Sequence; D006801:Humans; D007214:Indonesia; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000379:methods*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012480:Salmonella Infections / Q000382:microbiology*; D012485:Salmonella typhi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
Salmonella Typhi; bacterial molecular typing; clonal complex; insertion sequence IS200; multilocus sequence typing (MLST)