Relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss.
OBJECTIVE
To study the possible relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss (RPL).


METHODS
Descriptive study.


METHODS
University-affiliated tertiary teaching.


METHODS
A total of 22 couples with history of RPL and 20 fertile men.


METHODS
Semen samples from case and control men were examined for differences in semen parameters, DNA fragmentation, chromatin condensation, and sperm aneuploidy.


METHODS
Sperm DNA and chromatin integrity and sperm aneuploidy.


RESULTS
Sperm progressive motility (30.2% vs. 51.5%) was significantly lower and abnormal morphology (74.8% vs. 54.2%) was significantly higher in the RPL group versus the control group, respectively. The percentage of fragmented DNA was significantly increased in the RPL group (17.1% vs. 10.2%) as well as the rate of spermatozoa with nuclear chromatin decondensation (23.6% vs. 11.8%). There was a significantly higher sperm aneuploidy rate among the RPL group as well.


CONCLUSIONS
The increase in abnormal sperm parameters, sperm DNA fragmentation, nuclear chromatin decondensation, and sperm aneuploidy suggest possible causes of unexplained RPL.
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia. Electronic address: zidiines@hotmail.com.
Ines
I
Zidi-Jrah
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Amani
A
Hajlaoui
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Soumaya
S
Mougou-Zerelli
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Molka
M
Kammoun
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Imene
I
Meniaoui
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Amira
A
Sallem
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Sonia
S
Brahem
Department of Obstetrics and Gynecology, Farhat Hached University Hospital, Sousse, Tunisia.
Meriem
M
Fekih
Department of Obstetrics and Gynecology, Farhat Hached University Hospital, Sousse, Tunisia.
Mohammed
M
Bibi
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Ali
A
Saad
Department of Cytogenetic and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia.
Samira
S
Ibala-Romdhane
D000026:Abortion, Habitual / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D048430:Cell Shape; D042002:Chromatin Assembly and Disassembly; D004249:DNA Damage; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D018570:Risk Assessment; D012307:Risk Factors; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology*; D055815:Young Adult
DNA damage; Spermatozoa; aneuploidy; pregnancy loss