Genomic characteristics of coxsackievirus A8 strains associated with hand, foot, and mouth disease and herpangina.
Coxsackievirus A8 (CV-A8), a member of the genus Enterovirus of the family Picornaviridae, can cause a variety of infectious diseases, such as hand, foot and mouth disease (HFMD), herpangina (HA), encephalitis, paralysis, myelitis, and meningitis. This is a first report of complete genome sequences of CV-A8 strains associated with HFMD/HA since the prototype strain Donovan was identified in 1949. The complete genome sequences of eight new CV-A8 strains showed 19.2 %-20.6 % nucleotide differences when compared to the prototype strain Donovan, and 81.5 %-99.9 % similarity to each other. The topology of a polyphyletic tree based on complete capsid protein gene sequences indicated that the new CV-A8 strains and Donovan are monophyletic. However, seven CV-A8 strains clustered with CV-A10 and CV-A2 in the 5'UTR and P2 region, respectively. In the P3 region, three and four CV-A8 strains grouped with CV-A6 and CV-A2, respectively. Seven CV-A8 strains segregated from Donovan and grouped in a separate lineage in the 3'UTR. The strain CVA8/SZ266/CHN/2014 was most similar to EV71 in the nonstructural proteins regions. Phylogenetic analysis classified worldwide CV-A8 isolates into four distinct clusters, and almost all Chinese and Thai CV-A8 strains evolved independently in their respective lineages, which indicated geographical evolution of CV-A8.
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China. chen_l_2011@163.com.
Long
http://orcid.org/0000-0003-1110-6495
L
Chen
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China.
Hong
H
Yang
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China.
Chao
C
Wang
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China.
Xiang-Jie
XJ
Yao
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China.
Hai-Long
HL
Zhang
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China.
Ren-Li
RL
Zhang
Major Infectious Disease Control Key Laboratory and Shenzhen Public Service Platform of Pathogenic Microorganisms Repository, Shenzhen Center for Disease Control and Prevention, Shenzhen, 518055, People's Republic of China. heyaqing1019@126.com.
Ya-Qing
YQ
He
D001482:Base Composition; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D016679:Genome, Viral; D023281:Genomics; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006557:Herpangina / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny
D036022:Capsid Proteins