Genomic Characteristics of NDM-Producing Enterobacteriaceae Isolates in Australia and Their blaNDM Genetic Contexts.
blaNDM has been reported in different Enterobacteriaceae species and on numerous plasmid replicon types (Inc). Plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. The genetic context of blaNDM is heterogeneous. In this study, we genomically characterized 12 NDM-producing Enterobacteriaceae isolated in Australia between 2012 and 2014: Escherichia coli (n = 6), Klebsiella pneumoniae (n = 3), Enterobacter cloacae (n = 2) and Providencia rettgeri (n = 1). We describe their blaNDM genetic contexts within Tn125, providing insights into the acquisition of blaNDM into Enterobacteriaceae. IncFII-type (n = 7) and IncX3 (n = 4) plasmids were the most common plasmid types found. The IncHI1B (n = 1) plasmid was also identified. Five different blaNDM genetic contexts were identified, indicating four particular plasmids with specific blaNDM genetic contexts (NGCs), three of which were IncFII plasmids (FII-A to -C). Of note, the blaNDM genetic context of P. rettgeri was not conjugative. Epidemiological links between our NDM-producing Enterobacteriaceae were established by their acquisition of these five particular plasmid types. The combination of different molecular and genetic characterization methods allowed us to provide insight into the spread of plasmids transmitting blaNDM.
University of Queensland, University of Queensland Centre for Clinical Research, Herston, Queensland, Australia.
Alexander M
AM
Wailan
University of Queensland, University of Queensland Centre for Clinical Research, Herston, Queensland, Australia Pathology Queensland, Brisbane, Australia.
David L
DL
Paterson
Canberra Hospital, Australian National University Medical School, Australian Capital Territory, Australia.
Karina
K
Kennedy
Department of Microbiology and Infectious Diseases, Royal Perth Hospital, Perth, Australia.
Paul R
PR
Ingram
University of Queensland, University of Queensland Centre for Clinical Research, Herston, Queensland, Australia Sullivan Nicolaides Pathology, Brisbane, Queensland, Australia.
Evan
E
Bursle
University of Queensland, University of Queensland Centre for Clinical Research, Herston, Queensland, Australia h.sidjabat@uq.edu.au.
Hanna E
HE
Sidjabat
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001315:Australia / Q000453:epidemiology; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D016680:Genome, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000737:chemistry / Q000145:classification* / Q000378:metabolism; D011532:Providencia / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D001618:beta-Lactamases