Precision chemical heating for diagnostic devices.
Decoupling nucleic acid amplification assays from infrastructure requirements such as grid electricity is critical for providing effective diagnosis and treatment at the point of care in low-resource settings. Here, we outline a complete strategy for the design of electricity-free precision heaters compatible with medical diagnostic applications requiring isothermal conditions, including nucleic acid amplification and lysis. Low-cost, highly energy dense components with better end-of-life disposal options than conventional batteries are proposed as an alternative to conventional heating methods to satisfy the unique needs of point of care use.
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. buserj@uw.edu.
J R
JR
Buser
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
S
S
Diesburg
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
J
J
Singleton
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
D
D
Guelig
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. buserj@uw.edu.
J D
JD
Bishop
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
C
C
Zentner
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
R
R
Burton
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
P
P
LaBarre
Department of Bioengineering, University of Washington, Box 355061, Seattle, WA, USA. buserj@uw.edu.
P
P
Yager
PATH: The Program for Appropriate Technology in Healthcare, Seattle, WA, USA.
B H
BH
Weigl
D019937:Diagnostic Techniques and Procedures / Q000295:instrumentation*; D011211:Electric Power Supplies; D004867:Equipment Design; D006361:Heating; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation; D019095:Point-of-Care Systems