Bone stroma-derived cells change coregulators recruitment to androgen receptor and decrease cell proliferation in androgen-sensitive and castration-resistant prostate cancer cells.
Prostate cancer (CaP) bone metastasis is an early event that remains inactive until later-stage progression. Reduced levels of circulating androgens, due to andropause or androgen deprivation therapies, alter androgen receptor (AR) coactivator expression. Coactivators shift the balance towards enhanced AR-mediated gene transcription that promotes progression to androgen-resistance. Disruptions in coregulators may represent a molecular switch that reactivates latent bone metastasis. Changes in AR-mediated transcription in androgen-sensitive LNCaP and androgen-resistant C4-2 cells were analyzed for AR coregulator recruitment in co-culture with Saos-2 and THP-1. The Saos-2 cell line derived from human osteosarcoma and THP-1 cell line representing human monocytes were used to display osteoblast and osteoclast activity. Increased AR activity in androgen-resistant C4-2 was due to increased AR expression and SRC1/TIF2 recruitment and decreased SMRT/NCoR expression. AR activity in both cell types was decreased over 90% when co-cultured with Saos-2 or THP-1 due to dissociation of AR from the SRC1/TIF2 and SMRT/NCoR coregulators complex, in a ligand-dependent and cell-type specific manner. In the absence of androgens, Saos-2 decreased while THP-1 increased proliferation of LNCaP cells. In contrast, both Saos-2 and THP-1 decreased proliferation of C4-2 in absence and presence of androgens. Global changes in gene expression from both CaP cell lines identified potential cell cycle and androgen regulated genes as mechanisms for changes in cell proliferation and AR-mediated transactivation in the context of bone marrow stroma cells.
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Marcelo A
MA
Villagran
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Francisco A
FA
Gutierrez-Castro
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Diego F
DF
Pantoja
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Jose C
JC
Alarcon
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Macarena A
MA
Fariña
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Romina F
RF
Amigo
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Natalia A
NA
Muñoz-Godoy
Department of Medical Specialties, School of Medicine, University of Concepcion, Concepcion, Chile.
Mabel G
MG
Pinilla
Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion, Chile.
Eduardo A
EA
Peña
Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion, Chile.
Ivan
I
Gonzalez-Chavarria
Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion, Chile.
Jorge R
JR
Toledo
Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion, Chile.
Coralia I
CI
Rivas
Department of Physiopathology, School of Biological Sciences, University of Concepcion, Concepcion, Chile.
Juan C
JC
Vera
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile.
Eileen M
EM
McNerney
Molecular Endocrinology and Oncology Laboratory, University of Concepcion, Concepcion, Chile; Department of Medical Specialties, School of Medicine, University of Concepcion, Concepcion, Chile; Department of Urology, State University of New York at Buffalo, NY, USA. Electronic address: sergio.onate@udec.cl.
Sergio A
SA
Onate
D000728:Androgens / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D064129:Prostatic Neoplasms, Castration-Resistant / Q000473:pathology*; D011944:Receptors, Androgen / Q000378:metabolism*
D000728:Androgens; D011944:Receptors, Androgen
Androgens; Bone stroma; Prostate cancer; Transcription