Lipid Fraction and Intracellular Metabolite Analysis Reveal the Mechanism of Arachidonic Acid-Rich Oil Accumulation in the Aging Process of Mortierella alpina.
The mechanism of arachidonic acid (ARA) content increase during aging of Mortierella alpina was elucidated. Lipid fraction analysis showed that ARA content increased from 46.9% to 66.4% in the triacylglycerol (TAG) molecule, and ARA residue occupation increased in the majority of TAG molecules during the aging process. For the first time, intracellular metabolite analysis was conducted to reveal the pathways closely associated with ARA biosynthesis during aging. The main reason for the increased ARA content was not only at the expense of other fatty acids degradation but also at the expense of further ARA biosynthesis during aging. Furthermore, translocation played a vital role in ARA redistribution among the glycerol moiety, and mycelium did not die immediately with key pathways activated to maintain a relatively stable intracellular environment. This study lays a foundation for further improvement of ARA content in the oil product obtained from M. alpina.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Ai-Hui
AH
Zhang
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Xiao-Jun
XJ
Ji
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Wen-Jia
WJ
Wu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Lu-Jing
LJ
Ren
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Ya-Dong
YD
Yu
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
He
H
Huang
D016718:Arachidonic Acid / Q000032:analysis / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D020094:Mortierella / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D016718:Arachidonic Acid
Mortierella alpina; aging; arachidonic acid; intracellular metabolite; lipid fraction