The upregulation of miRNA-146a inhibited biological behaviors of ESCC through inhibition of IRS2.
In recent years, microRNAs, also called as miRNAs, play an important role in carcinogenesis, and the dysregulation of miRNAs is closely associated with cancer progression. Till now, little has been known about the role of miRNA-146a in the esophageal squamous cell carcinomas (ESCC). In the present study, we used in vitro assays to investigate the mechanisms of miRNA-146a in ESCC cell lines and 60 ESCC tissues. Here, we found that miRNA-146a expression is downregulated in both ESCC cell lines and tissues and obviously associated with pathological indicators, such as metastasis and stage of ESCC. In addition, the overexpression of miRNA-146a suppressed EC109 and TE8 cell proliferation and invasion. Meanwhile, miRNA-146a overexpression extremely inhibited the protein expression of insulin receptor substrate 2 (IRS2). Notably, the enforced expression of IRS2 in EC109 cells with miRNA-146a overexpression attenuated the inhibitory effects of miRNA-146a. In conclusion, our findings suggest that miRNA-146a may function as a useful clinical tool in the treatment and diagnosis of ESCC, and its overexpression suppressed cell growth through inhibition of IRS2. Thus, miRNA-146a pathway may be recommended as potential makers for drug design.
Department of Thoracic Surgery, Jinan Central Hospital, Shandong University, No. 105 Jiefang Road, Jinan, Shandong, 250013, People's Republic of China.
Haibo
H
Liu
Department of Oncology, Shandong Cancer Hospital and Institute, No. 440 Jiyan Road, Jinan, Shandong, 250117, People's Republic of China.|Department of Oncology, Affiliated Hospital of Shandong Academy of Medical Sciences, No. 38 Wuyingshan Road, Jinan, Shandong, 250031, People's Republic of China.
Guohua
G
Ren
Department of Thoracic Surgery, Jinan Central Hospital, Shandong University, No. 105 Jiefang Road, Jinan, Shandong, 250013, People's Republic of China.
Liangming
L
Zhu
Department of Thoracic Surgery, Shandong Provincial Hospital, Shandong University, No. 324 Jingwuweiqi Road, Jinan, Shandong, 250021, People's Republic of China. liuxyshandong@163.com.
Xiangyan
X
Liu
Department of Thoracic Surgery, Shandong Provincial Hospital, Shandong University, No. 324 Jingwuweiqi Road, Jinan, Shandong, 250021, People's Republic of China. sdhxpshengli@163.com.
Xiaopeng
X
He
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D015854:Up-Regulation
C526297:IRS2 protein, human; D055504:Insulin Receptor Substrate Proteins; C542542:MIRN146 microRNA, human; D035683:MicroRNAs
ESCC; IRS2; miRNA-146a