Engineering DNA Three-Way Junction with Multifunctional Moieties: Sensing Platform for Bioanalysis.
Functionalization of DNA nanostructures is critical to the achievement of the application in biosensors. Herein, we demonstrate a novel DNA three-way junction (TWJ) with three functional moieties, which integrates the electrochemical, fluorescent, and colorimetric properties. Upon addition of external stimuli, including DNA, thrombin, and ATP, the specific interactions between targets and sensing elements could induce strand displacement reaction and conformation transformation, resulting in the integration of G-quadruplex/hemin complex as electrochemical probe, lighting up the fluorescence of DNA/Ag nanoclusters and enhancing the catalytic activity of DNAzyme to catalyze the H2O2-TMB system to generate colorimetric signal. Such a functional DNA nanostructure actually serves as a biosensing platform, showing high sensitivity and selectivity for DNA, thrombin, and ATP detection. Furthermore, We also show that this novel sensing platform can be utilized to detect three different kinds of targets independently and simultaneously. Our integrated concept provides a paradigm for exploring the potential of TWJs in analytical applications.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Libing
L
Zhang
Materials Science and Engineering, College of Engineering, Peking University , Beijing 100871, China.
Shaojun
S
Guo
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Jinbo
J
Zhu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Zhixue
Z
Zhou
Department of Chemistry, University of Science and Technology of China , Hefei, Anhui 230026, China.
Tao
T
Li
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Jing
J
Li
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Shaojun
S
Dong
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, China.
Erkang
E
Wang
D000255:Adenosine Triphosphate / Q000032:analysis; D015374:Biosensing Techniques; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D005453:Fluorescence; D049329:Nanostructures / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013917:Thrombin / Q000032:analysis
D012834:Silver; D000255:Adenosine Triphosphate; D004247:DNA; D013917:Thrombin