[Determination of Flavonoids in Cycas revoluta Leaves by Chemiluminescence-Flow Injection Analysis Method].
OBJECTIVE
To detect flavonoids from Cycas revoluta leaves by means of Chemiluminescence-Flow Injection Analysis (CL-FIA).


METHODS
Under alkaline condition, a CL-FIA method was established to determine flavonoids from leaves of Cycas revoluta on the basis of inhibiting effect of flavonoids to the Luminol-H2O2-Cu2+ chemiluminescence system and the reversed flow injection technique.


RESULTS
In the range of 2. 0 x 10(-6) ~ 1. 0 x 10(-3) mg/mL, the decrease of CL intensity was correlated with flavonoids concentration while the detection limit was 0. 0265 µg/mL. Under the optimized conditions, the flavonoids of Cycas revoluta leaves were detected with its average rate reaching 1. 61% and RSD 1. 32%.


CONCLUSIONS
Through the interference test and compared with the data of CL-FIA and UV, it is concluded that CL-FIA can be used in the analysis and detection of flavonoids from Cycas revoluta leaves.
Hong-yan
HY
Shi
Hai-ping
HP
Zhu
Ya-juan
YJ
Qu
Pei-jun
PJ
Yang
D029784:Cycas / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D017022:Flow Injection Analysis; D057230:Limit of Detection; D008163:Luminescent Measurements; D008165:Luminol; D018515:Plant Leaves / Q000737:chemistry*
D005419:Flavonoids; D008165:Luminol