Dataset of natural antisense transcripts in P. vivax clinical isolates derived using custom designed strand-specific microarray.
Natural antisense transcripts (NATs) have been detected in many organisms and shown to regulate gene expression. Similarly, NATs have also been observed in malaria parasites with most studies focused on Plasmodium falciparum. There were no reports on the presence of NATs in Plasmodium vivax, which has also been shown to cause severe malaria like P. falciparum, until a recent study published by us. To identify in vivo prevalence of antisense transcripts in P. vivax clinical isolates, we performed whole genome expression profiling using a custom designed strand-specific microarray that contains probes for both sense and antisense strands. Here we describe the experimental methods and analysis of the microarray data available in Gene Expression Omnibus (GEO) under GSE45165. Our data provides a resource for exploring the presence of antisense transcripts in P. vivax isolated from patients showing varying clinical symptoms. Related information about the description and interpretation of the data can be found in a recent publication by Boopathi and colleagues in Infection, Genetics and Evolution 2013.
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
P A
PA
Boopathi
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Amit Kumar
AK
Subudhi
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Shilpi
S
Garg
Department of Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.
Sheetal
S
Middha
Department of Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.
Jyoti
J
Acharya
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Deepak
D
Pakalapati
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Vishal
V
Saxena
Genotypic Technology Pvt. Ltd., Bangalore, India.
Mohammed
M
Aiyaz
Genotypic Technology Pvt. Ltd., Bangalore, India.
Bipin
B
Chand
Genotypic Technology Pvt. Ltd., Bangalore, India.
Raja C
RC
Mugasimangalam
Department of Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.
Sanjay K
SK
Kochar
Department of Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.
Parmendra
P
Sirohi
Rajasthan University of Health Sciences, Jaipur, Rajasthan, India.
Dhanpat K
DK
Kochar
Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Ashis
A
Das
Complicated malaria; Natural antisense transcripts; Plasmodium vivax; Strand specific microarray; Uncomplicated malaria