Production of immunoregulatory polysaccharides from Crassostrea hongkongensis and their positive effects as a nutrition factor in modulating the effectiveness and toxicity of 5-FU chemotherapy in mice.
Chemotherapy is generally accompanied by undesirable side effects, such as immunosuppression and malnutrition, which reduce tolerance to cancer therapies. Prior studies have shown that immunonutrition improves the clinical outcomes of cancer patients. In this study, immunoregulatory polysaccharides from Crassostrea hongkongensis were included in a nutrition formula that was administered to S180 tumor-bearing mice in combination with 5-fluorouracil (5-FU) treatment. The C30-60% fraction of the polysaccharides was characterized as a branched polysaccharide, with a high amount of d-glucose (96.76% of the total) and the highest uronic acid and sulfate groups' content among all of the polysaccharide fractions. The C30-60% polysaccharide fraction showed a maximal proliferative effect on RAW264.7 cells and T lymphocytes at a concentration of 0.0391 mg mL(-1) and 0.0781 mg mL(-1), respectively. Moreover, the combination treatment of the C30-60% polysaccharide-based nutrition formula (OPNF) with the administration of 5-FU effectively inhibited the growth of tumors and notably increased the leucocyte and lymphocyte counts in S180 tumor-bearing mice. In addition, a slight increase in the erythrocyte and hemoglobin values was observed in the mice treated with the combination of OPNF and 5-FU. These results suggest that supplementation with a C30-60%-based enteral formula would be beneficial for patients undergoing chemotherapy with 5-FU.
Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164-Xingangxi Road, Guangzhou, 510301, China. jypan@scsio.ac.cn.
Bingna
B
Cai
Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164-Xingangxi Road, Guangzhou, 510301, China. jypan@scsio.ac.cn.
Hua
H
Chen
Rutgers University, 98 Brett Road, Piscataway, New Jersey 08854, USA.
Han
H
Sun
Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164-Xingangxi Road, Guangzhou, 510301, China. jypan@scsio.ac.cn.
Peng
P
Wan
Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164-Xingangxi Road, Guangzhou, 510301, China. jypan@scsio.ac.cn.
Huili
H
Sun
Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164-Xingangxi Road, Guangzhou, 510301, China. jypan@scsio.ac.cn.
Jianyu
J
Pan
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D049896:Crassostrea / Q000737:chemistry* / Q000378:metabolism; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000494:pharmacology / Q000633:toxicity*; D056747:Immunomodulation; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D000067996:RAW 264.7 Cells; D011897:Random Allocation; D012509:Sarcoma / Q000188:drug therapy*; D013154:Spleen; D013950:Thymus Gland / Q000187:drug effects
D000970:Antineoplastic Agents; D011134:Polysaccharides; D005472:Fluorouracil