DNA-binding proteins from marine bacteria expand the known sequence diversity of TALE-like repeats.
Transcription Activator-Like Effectors (TALEs) of Xanthomonas bacteria are programmable DNA binding proteins with unprecedented target specificity. Comparative studies into TALE repeat structure and function are hindered by the limited sequence variation among TALE repeats. More sequence-diverse TALE-like proteins are known from Ralstonia solanacearum (RipTALs) and Burkholderia rhizoxinica (Bats), but RipTAL and Bat repeats are conserved with those of TALEs around the DNA-binding residue. We study two novel marine-organism TALE-like proteins (MOrTL1 and MOrTL2), the first to date of non-terrestrial origin. We have assessed their DNA-binding properties and modelled repeat structures. We found that repeats from these proteins mediate sequence specific DNA binding conforming to the TALE code, despite low sequence similarity to TALE repeats, and with novel residues around the BSR. However, MOrTL1 repeats show greater sequence discriminating power than MOrTL2 repeats. Sequence alignments show that there are only three residues conserved between repeats of all TALE-like proteins including the two new additions. This conserved motif could prove useful as an identifier for future TALE-likes. Additionally, comparing MOrTL repeats with those of other TALE-likes suggests a common evolutionary origin for the TALEs, RipTALs and Bats.
Department of General Genetics, Centre for Plant Molecular Biology, University of Tuebingen, Auf der Morgenstelle 32, Tuebingen, Baden-Wuerttemberg, 72076, Germany.
Orlando
http://orcid.org/0000-0002-7264-4484
O
de Lange
Department of General Genetics, Centre for Plant Molecular Biology, University of Tuebingen, Auf der Morgenstelle 32, Tuebingen, Baden-Wuerttemberg, 72076, Germany.
Christina
C
Wolf
Department of Computer Science and Centre for Bioinformatics, University of Tuebingen, Sand 14, Tuebingen, Baden-Wuerttemberg, 72076, Germany.
Philipp
P
Thiel
Department of Computer Science and Centre for Bioinformatics, University of Tuebingen, Sand 14, Tuebingen, Baden-Wuerttemberg, 72076, Germany.
Jens
J
Krüger
NanoTemper Technologies, Munich, 81369, Germany.
Christian
C
Kleusch
Department of Computer Science and Centre for Bioinformatics, University of Tuebingen, Sand 14, Tuebingen, Baden-Wuerttemberg, 72076, Germany Quantitative Biology Centre and Faculty of Medicine, University of Tuebingen, Sand 14, Tuebingen, Baden-Wuerttemberg, 72076, Germany.
Oliver
O
Kohlbacher
Department of General Genetics, Centre for Plant Molecular Biology, University of Tuebingen, Auf der Morgenstelle 32, Tuebingen, Baden-Wuerttemberg, 72076, Germany thomas.lahaye@zmbp.uni-tuebingen.de.
Thomas
T
Lahaye
D059001:Aquatic Organisms; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014644:Genetic Variation; D056186:Metagenomics; D011485:Protein Binding; D055550:Protein Stability; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D040681:Structural Homology, Protein
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA