Distribution of single wall carbon nanotubes in the Xenopus laevis embryo after microinjection.
Single wall carbon nanotubes (SWCNTs) are advanced materials with the potential for a myriad of diverse applications, including biological technologies and large-scale usage with the potential for environmental impacts. SWCNTs have been exposed to developing organisms to determine their effects on embryogenesis, and results have been inconsistent arising, in part, from differing material quality, dispersion status, material size, impurity from catalysts and stability. For this study, we utilized highly purified SWCNT samples with short, uniform lengths (145 ± 17 nm) well dispersed in solution. To test high exposure doses, we microinjected > 500 µg ml(-1) SWCNT concentrations into the well-established embryogenesis model, Xenopus laevis, and determined embryo compatibility and subcellular localization during development. SWCNTs localized within cellular progeny of the microinjected cells, but were heterogeneously distributed throughout the target-injected tissue. Co-registering unique Raman spectral intensity of SWCNTs with images of fluorescently labeled subcellular compartments demonstrated that even at regions of highest SWCNT concentration, there were no gross alterations to subcellular microstructures, including filamentous actin, endoplasmic reticulum and vesicles. Furthermore, SWCNTs did not aggregate and localized to the perinuclear subcellular region. Combined, these results suggest that purified and dispersed SWCNTs are not toxic to X. laevis animal cap ectoderm and may be suitable candidate materials for biological applications.
Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Brian D
BD
Holt
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Joseph H
JH
Shawky
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.|Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Kris Noel
KN
Dahl
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Lance A
LA
Davidson
Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Mohammad F
MF
Islam
D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D008845:Microinjections; D018613:Microscopy, Confocal; D037742:Nanotubes, Carbon / Q000737:chemistry / Q000633:toxicity*; D012709:Serum Albumin / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D014982:Xenopus laevis / Q000196:embryology*
D037742:Nanotubes, Carbon; D012709:Serum Albumin
Raman spectroscopy; Xenopus laevis; actin; bovine serum albumin; carbon nanotube; development; nanoparticle toxicity