Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA).
Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (~9.99%), 5302 (~9.45%), 5242 (~9.35%), 4407 (~7.86%) and 4195 (~7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Zhengquan
Z
Gao
College of Marine and Environmental Sciences, James Cook University, Douglas, 4811, Australia; School of Food and Agriculture Sciences, University of Queensland, St. Lucia, 4072, Australia.
Yan
Y
Li
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Guanxun
G
Wu
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Guoqiang
G
Li
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Haifeng
H
Sun
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Suzhen
S
Deng
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Yicheng
Y
Shen
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Guoqiang
G
Chen
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Ruihao
R
Zhang
School of Life Sciences, Shandong University of Technology, Zibo, 255049, P.R. China.
Chunxiao
C
Meng
Yellow Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P.R. China.
Xiaowen
X
Zhang
D000460:Chlorophyta / Q000187:drug effects / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D058086:Microalgae / Q000187:drug effects / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology*; D059467:Transcriptome; D015854:Up-Regulation; D024341:Xanthophylls / Q000235:genetics
D003517:Cyclopentanes; D054883:Oxylipins; D024341:Xanthophylls; C011006:jasmonic acid; C005948:astaxanthine; D020156:Salicylic Acid