Pyrazinamide resistance determined by liquid culture at low pH better correlates with genetic mutations in MDR tuberculosis isolates.
We detected the pyrazinamide (PZA) susceptibility of Mycobacterium tuberculosis strains in the Bactec MGIT 960 liquid medium with different pH values. Our results demonstrated that PZA resistance determined by liquid culture at pH 5.7 (94.9%) showed better correlation with genetic changes among multidrug-resistant M. tuberculosis isolates (P=0.001).
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: pangyu@chinatb.org.
Yu
Y
Pang
Qingdao Center for Disease Control and Prevention, Qingdao, Shangdong, China.
Zhongdong
Z
Wang
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Huiwen
H
Zheng
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Yuanyuan
Y
Song
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Yufeng
Y
Wang
National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: zhaoyanlin@chinatb.org.
Yanlin
Y
Zhao
D000995:Antitubercular Agents / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D011718:Pyrazinamide / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology*
D000995:Antitubercular Agents; D011718:Pyrazinamide
Drug resistance; MGIT; Mycobacterium tuberculosis; Pyrazinamide