Obestatin stimulates differentiation and regulates lipolysis and leptin secretion in rat preadipocytes.
Obestatin is a 23-amino acid peptide encoded by the ghrelin gene, which regulates food intake, body weight and insulin sensitivity. Obestatin influences glucose and lipid metabolism in mature adipocytes in rodents. However, the role of this peptide in rat preadipocytes remains to be fully understood. The current study characterized the effects of obestatin on lipid accumulation, preadipocyte differentiation, lipolysis and leptin secretion in rat primary preadipocytes. Obestatin enhanced lipid accumulation in rat preadipocytes and increased the expression of surrogate markers of preadipocyte differentiation. At the early stage of differentiation, obestatin suppressed lipolysis. By contrast, lipolysis was stimulated at the late stage of adipogenesis. Furthermore, obestatin stimulated the release of leptin, a key satiety hormone. Overall, the results indicated that obestatin promotes preadipocyte differentiation. Obestatin increased leptin release in preadipocytes, while the modulation of lipolysis appears to depend upon the stage of differentiation.
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Tatiana
T
Wojciechowicz
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Marek
M
Skrzypski
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Paweł A
PA
Kołodziejski
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Dawid
D
Szczepankiewicz
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Ewa
E
Pruszyńska-Oszmałek
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Przemysław
P
Kaczmarek
Department of Hepatology and Gastroenterology and the Interdisciplinary Centre of Metabolism: Endocrinology, Diabetes and Metabolism, Charite‑University Medicine Berlin, Berlin D‑13353, Germany.
Mathias Z
MZ
Strowski
Department of Animal Physiology and Biochemistry, Poznań University of Life Sciences, Poznań 60‑637, Poland.
Krzysztof W
KW
Nowak
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D020738:Leptin / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008066:Lipolysis / Q000235:genetics*; D008297:Male; D036361:Peptide Hormones / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000378:metabolism
D020738:Leptin; D036361:Peptide Hormones; D014280:Triglycerides; C505798:obestatin, rat