A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags.
A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Jessica A
JA
Stewart
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Brent F
BF
Piligian
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Sarah R
SR
Rundell
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. ben.swarts@cmich.edu.
Benjamin M
BM
Swarts
D000345:Affinity Labels; D001386:Azides / Q000737:chemistry*; D055668:Photochemical Processes; D014466:Ultraviolet Rays
D000345:Affinity Labels; D001386:Azides