A microarray analysis of two distinct lymphatic endothelial cell populations.
We have recently identified lymphatic endothelial cells (LECs) to form two morphologically different populations, exhibiting significantly different surface protein expression levels of podoplanin, a major surface marker for this cell type. In vitro shockwave treatment (IVSWT) of LECs resulted in enrichment of the podoplanin(high) cell population and was accompanied by markedly increased cell proliferation, as well as 2D and 3D migration. Gene expression profiles of these distinct populations were established using Affymetrix microarray analyses. Here we provide additional details about our dataset (NCBI GEO accession number GSE62510) and describe how we analyzed the data to identify differently expressed genes in these two LEC populations.
Skin and Endothelium Research Division, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
Bernhard
B
Schweighofer
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria ; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Sabrina
S
Rohringer
Red Cross Blood Transfusion Service of Upper Austria, Linz, Austria.
Johannes
J
Pröll
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria ; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Wolfgang
W
Holnthoner