Improvement of identification of Capnocytophaga canimorsus by matrix-assisted laser desorption ionization-time of flight mass spectrometry using enriched database.
Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.
Laboratory of Clinical Microbiology, CHU Dinant-Godinne UCL Namur, B-5530, Yvoir, Belgium.
Amandine
A
Magnette
Laboratory of Clinical Microbiology, CHU Dinant-Godinne UCL Namur, B-5530, Yvoir, Belgium. Electronic address: te-din.huang@uclouvain.be.
Te-Din
TD
Huang
Research Unit in Biology of Microorganisms, Namur Institute for Life Sciences (NARILIS), University of Namur, B-5000, Namur, Belgium.
Francesco
F
Renzi
Laboratory of Clinical Microbiology, CHU Dinant-Godinne UCL Namur, B-5530, Yvoir, Belgium.
Pierre
P
Bogaerts
Research Unit in Biology of Microorganisms, Namur Institute for Life Sciences (NARILIS), University of Namur, B-5000, Namur, Belgium.
Guy R
GR
Cornelis
Laboratory of Clinical Microbiology, CHU Dinant-Godinne UCL Namur, B-5530, Yvoir, Belgium.
Youri
Y
Glupczynski
D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002206:Capnocytophaga / Q000737:chemistry / Q000302:isolation & purification*; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004285:Dogs; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology / Q000662:veterinary*; D006801:Humans; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015047:Zoonoses / Q000175:diagnosis* / Q000382:microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
Capnocytophaga; Capnocytophaga canimorsus; Capnocytophaga cynodegmi; Database; MALDI-TOF MS; Rapid identification