Prevalence of Mycobacterium lentiflavum in cystic fibrosis patients, France.
BACKGROUND
Mycobacterium lentiflavum is rarely isolated in respiratory tract samples from cystic fibrosis patients. We herein describe an unusually high prevalence of M. lentiflavum in such patients.


METHODS
M. lentiflavum, isolated from the respiratory tract of cystic fibrosis patients, was identified using both rpoB partial sequencing and detected directly in the sputum by using real-time PCR targeting the smpB gene.


RESULTS
M. lentiflavum emerged as the third most prevalent nontuberculous mycobacterial species isolated in cystic fibrosis patients in Marseille, France. Six such patients were all male, and two of them may have fulfilled the American Thoracic Society clinical and microbiological criteria for M. lentiflavum potential lung infection.


CONCLUSIONS
M. lentiflavum was the third most common mycobacteria isolated in cystic fibrosis patients, particularly in six male patients. M. lentiflavum outbreaks are emerging particularly in cystic fibrosis patients.
Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, INSERM 1095. Faculté de Médecine, Marseille, 13005, France. michaelphelippeau@gmail.com.
Michael
M
Phelippeau
Centre de Ressource et de Compétences de la Mucoviscidose (CRCM) pédiatrique CHU Hôpital la Timone, Marseille, France. JeanChristophe.DUBUS@ap-hm.fr.
Jean-Christophe
JC
Dubus
Centre de Ressource et de Compétences de la Mucoviscidose (CRCM) adulte; équipe de Transplantation pulmonaire, CHU Hôpital Nord, URMITE - CNRS-UMR 6236 Aix-Marseille Université, Marseille, France. MartineLouise.REYNAUD@ap-hm.fr.
Martine
M
Reynaud-Gaubert
Centre de Ressource et de Compétences de la Mucoviscidose (CRCM) adulte; équipe de Transplantation pulmonaire, CHU Hôpital Nord, URMITE - CNRS-UMR 6236 Aix-Marseille Université, Marseille, France. carine.gomez@ap-hm.fr.
Carine
C
Gomez
Centre de Ressource et de Compétences de la Mucoviscidose (CRCM) pédiatrique CHU Hôpital la Timone, Marseille, France. Nathalie.STREMLER@ap-hm.fr.
Nathalie
N
Stremler le Bel
Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, INSERM 1095. Faculté de Médecine, Marseille, 13005, France. marielle.bedotto@gmail.com.
Marielle
M
Bedotto
Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, INSERM 1095. Faculté de Médecine, Marseille, 13005, France. elsa.prudent@univ-amu.fr.
Elsa
E
Prudent
Aix Marseille Université, URMITE, UMR CNRS 7278, IRD 198, INSERM 1095. Faculté de Médecine, Marseille, 13005, France. michel.drancourt@univ-amu.fr.|Unité de recherche sur les maladies infectieuses et tropicales émergentes, Faculté de Médecine, 27 Bd jean Moulin, 13385, Marseille, cedex 5, France. michel.drancourt@univ-amu.fr.
Michel
M
Drancourt
D000293:Adolescent; D000328:Adult; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology; D003550:Cystic Fibrosis / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005602:France / Q000453:epidemiology; D006801:Humans; D008297:Male; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000235:genetics / Q000302:isolation & purification; D015995:Prevalence; D016601:RNA-Binding Proteins / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012137:Respiratory System / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000382:microbiology; D055815:Young Adult
D016601:RNA-Binding Proteins; C430328:small protein B