Comparative and quantitative proteomics reveal the adaptive strategies of oyster larvae to ocean acidification.
Decreasing pH due to anthropogenic CO2 inputs, called ocean acidification (OA), can make coastal environments unfavorable for oysters. This is a serious socioeconomical issue for China which supplies >70% of the world's edible oysters. Here, we present an iTRAQ-based protein profiling approach for the detection and quantification of proteome changes under OA in the early life stage of a commercially important oyster, Crassostrea hongkongensis. Availability of complete genome sequence for the pacific oyster (Crassostrea gigas) enabled us to confidently quantify over 1500 proteins in larval oysters. Over 7% of the proteome was altered in response to OA at pHNBS 7.6. Analysis of differentially expressed proteins and their associated functional pathways showed an upregulation of proteins involved in calcification, metabolic processes, and oxidative stress, each of which may be important in physiological adaptation of this species to OA. The downregulation of cytoskeletal and signal transduction proteins, on the other hand, might have impaired cellular dynamics and organelle development under OA. However, there were no significant detrimental effects in developmental processes such as metamorphic success. Implications of the differentially expressed proteins and metabolic pathways in the development of OA resistance in oyster larvae are discussed. The MS proteomics data have been deposited to the ProteomeXchange with identifiers PXD002138 (http://proteomecentral.proteomexchange.org/dataset/PXD002138).
The Swire Institute of Marine Sciences and School of Biological Sciences, The University of Hong Kong, Hong Kong Special Administrative Region, P. R. China.
R
R
Dineshram
Department of Chemistry, The University of Hong Kong, Hong Kong Special Administrative Region, P. R. China.
Q
Q
Quan
Department of Biochemistry, L.K.S Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, P. R. China.|Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong.
Rakesh
R
Sharma
Biological, Environmental Sciences & Engineering, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
Kondethimmanahalli
K
Chandramouli
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Hari Krishna
HK
Yalamanchili
Department of Chemistry, The University of Hong Kong, Hong Kong Special Administrative Region, P. R. China.
Ivan
I
Chu
The Swire Institute of Marine Sciences and School of Biological Sciences, The University of Hong Kong, Hong Kong Special Administrative Region, P. R. China.
Vengatesen
V
Thiyagarajan
D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D049896:Crassostrea / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007814:Larva / Q000378:metabolism; D020543:Proteome; D040901:Proteomics
D020543:Proteome
Climate change; Crassostrea hongkongensis; Metamorphosis; Ocean acidification; Oyster larvae; iTRAQ