Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry.
Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH4HCO3 was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue.
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Jia
J
Chen
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Xian-Long
XL
Cheng
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Feng
F
Wei
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Qian-Qian
QQ
Zhang
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Ming-Hua
MH
Li
National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, China.
Shuang-Cheng
SC
Ma