Analysis of Stem Cell Motility In Vivo Based on Immunodetection of Planarian Neoblasts and Tracing of BrdU-Labeled Cells After Partial Irradiation.
Planarian flatworms have become an important system for the study of stem cell behavior and regulation in vivo. These organisms are able to regenerate any part of their body upon damage or amputation. A crucial cellular event in the process of planarian regeneration is the migration of pluripotent stem cells (known as neoblasts) to the site of injury. Here we describe two approaches for analyzing migration of planarian stem cells to an area where these have been ablated by localized X-ray irradiation. The first approach involves immunolabeling of mitotic neoblasts, while the second is based on tracing stem cells and their progeny after BrdU incorporation. The use of planarians in studies of cell motility is suitable for the identification of factors that influence stem cell migration in vivo and is amenable to RNA interference or pharmacological screening.
Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, USA.
Junichi
J
Tasaki
Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, USA.
Chihiro
C
Uchiyama-Tasaki
Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, USA. labib.rouhana@wright.edu.
Labib
L
Rouhana
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002465:Cell Movement; D005455:Fluorescent Antibody Technique / Q000379:methods*; D010932:Planarians / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D014965:X-Rays
D001973:Bromodeoxyuridine
Cell migration; Cell motility; Mitosis; Neoblasts; Planarian; Platyhelminthes; Regeneration; Stem cells