Investigation of mucus obtained from different fish species on the acute pain induced with scalpel incision in paw of rats.
No comparative study could be found for the analgesic activity of mucuses from the Oncorhynchus mykiss (OM), Salvelinus fontinalis (SF), Salmo coruhensis (SC), Acipenser gueldenstaedtii (AG), and Acipenser baerii (AB) fish species in the literature. We aimed to investigate the effects of mucuses obtained from the abovementioned fish species on scalpel incision-induced pain in the rat paw and to examine the role of oxidant/antioxidant parameters and COX-2 gene expression in the analgesic activities. Animals were divided into groups: SIC (scalpel incision; SI), SIDS (SI+25 mg/kg diclofenac sodium), SOM (SI+25 mg/kg OM mucus), SFM (SI+25 mg/kg SF mucus), SCM (SI+25 mg/kg SC mucus), SAgM (SI+25 mg/kg AG mucus), SAbM (SI+25 mg/kg AB mucus), and HG (healthy). The paw pain thresholds were measured with a Basile algesimeter before and after diclofenac sodium (DS) or mucus administration, and then the rats were euthanized with thiopental sodium. Oxidant/antioxidant and COX-2 gene expression parameters were measured in paw tissues. OM, SC, AG, and AB fish mucuses could not decrease the SI-induced pain. However, SF fish mucus prevented this pain by 69% after the first hour and by 58.3% after the third hour. DS was shown to suppress pain more weakly than SF, preventing the pain by 62.1% and 50.0% after the first and third hours, respectively. SF mucus and DS significantly inhibited increase of COX-2 gene expression, while other fish mucuses could not. None of the fish mucuses except SF mucus in conjunction with DS could significantly inhibit the increase in oxidant parameters and decrease in antioxidants. SF fish mucus should be comparatively assessed in clinical practice for treatment of postoperative pain.
Department of Pharmacology, Faculty of Medicine, Erzincan University, 24030, Erzincan, Turkey.
Nihal
N
Cetin
Bahtiyar
B
Suleyman
Ufuk
U
Kuyrukluyildiz
Hatice Sevim
HS
Nalkiran
Altan
A
Kiran
Songul
S
Gencoglu
Ahmet
A
Duzgun
Ilker Zeki
IZ
Kurtoglu
Oguzhan
O
Yarali
Mehmet Ali
MA
Gul
Halis
H
Suleyman
D059787:Acute Pain / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D000700:Analgesics; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004008:Diclofenac / Q000627:therapeutic use; D005399:Fishes; D015870:Gene Expression; D008297:Male; D009093:Mucus; D016877:Oxidants / Q000378:metabolism; D010149:Postoperative Pain / Q000628:therapy*; D017208:Rats, Wistar
D000700:Analgesics; D000975:Antioxidants; D016877:Oxidants; D004008:Diclofenac; D051546:Cyclooxygenase 2