A Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Quantitation of 10 Bioactive Components in Rhus verniciflua Extracts.
The purpose of this study was to develop a simultaneous method to quantify 10 bioactive compounds in Rhus verniciflua extracts using high-performance liquid chromatography-tandem mass spectrometry. The chromatographic separation was performed using a C18 column under gradient elution with 0.1% formic acid and acetonitrile as the mobile phase solvents. The analytes were detected in the negative-ion mode using multiple-reaction monitoring detection with an electrospray ionization interface. The calibration curves for all the analytes showed good linearity (r(2) >0.997) over the concentration range of 1-1,000 ng/mL. The recovery values were within 89.53-110.14%, and the intra- and interday coefficients of variation were <4.86% for all the tested compounds. The developed method was successfully applied to a quality assessment of the R. verniciflua extract samples.
Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
Ming Ji
MJ
Jin
Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
In Sook
IS
Kim
Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea.
Shaheed Ur
SU
Rehman
School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
Mi-Sook
MS
Dong
Lifetree Biotech Co., Ltd., Suwon, Gyeonggi-do 441-350, Republic of Korea.
Chun-Soo
CS
Na
Institute of Pharmaceutical Science and Technology and College of Pharmacy, Hanyang University, Ansan, Gyeonggi-do 426-791, Republic of Korea yoohh@hanyang.ac.kr.
Hye Hyun
HH
Yoo
D000097:Acetonitriles; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards*; D005419:Flavonoids / Q000302:isolation & purification*; D005561:Formates; D005707:Gallic Acid / Q000302:isolation & purification*; D006801:Humans; D015588:Observer Variation; D010936:Plant Extracts / Q000737:chemistry*; D029045:Rhus / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards*
D000097:Acetonitriles; D005419:Flavonoids; D005561:Formates; D010936:Plant Extracts; D012997:Solvents; C030544:formic acid; D005707:Gallic Acid; C032159:acetonitrile