Chemopreventive activity of grape juice concentrate (G8000TM) on rat colon carcinogenesis induced by azoxymethane.
Colorectal cancer is the third most common cancer worldwide in both sexes, with similar geographic patterns between genders. This neoplasm has good prognosis if the disease is diagnosed at early stages. The aim of this study was to evaluate the effect of red grape juice on the expression of COX-2 and Ki-67 expression following colon carcinogenesis induced by azoxymethane (AOM). Thirty-five rats were randomly distributed into seven groups (n=5 per group): G1: SHAM or negative control received only saline; G2 (positive control): animals received 15 mg/kg AOM; G3: animals received 1% red grape juice 2 weeks before the administration of AOM; G4: animals received 2% red grape juice 2 weeks before the administration of AOM; G5: animals received 1% red grape juice 4 weeks after the last administration of AOM; G6: animals received 2% red grape juice 4 weeks after the last administration of AOM; G7: animals received only 2% red grape juice. COX-2 mRNA expression was reduced in animals treated with 1% red grape juice before AOM induction or 2% red grape juice after AOM induction. COX-2 immunoexpression was also reduced to groups treated with red grape juice at 1% before and after AOM induction or 2% red grape juice after AOM induction. Decreased immunoexpression of Ki-67 positive cells was observed in animals treated with 1% grape juice before AOM-treated animals. Taken together, grape juice concentrate is able to exert some chemopreventive activity on rat colon carcinogenesis.
Oncology Division, Medicine Department, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Roseane Mendes
RM
Silva
Oncology Division, Medicine Department, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Vanessa Maria de Lima Pazine
VM
Campanholo
Department of Pathology, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Ana Paula Ribeiro
AP
Paiotti
Department of Pathology, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Ricardo
R
Artigiani Neto
Department of Pathology, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Celina Tizuko Fujiyama
CT
Oshima
Department of Biosciences, Universidade Federal de São Paulo UNIFESP, Santos, SP, Brazil. Electronic address: daribeiro@unifesp.br.
Daniel Araki
DA
Ribeiro
Oncology Division, Medicine Department, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil.
Nora Manoukian
NM
Forones
D000818:Animals; D001397:Azoxymethane; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D000067030:Fruit and Vegetable Juices; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019394:Ki-67 Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D027843:Vitis / Q000737:chemistry*
D019394:Ki-67 Antigen; D010936:Plant Extracts; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D001397:Azoxymethane
Cell proliferation; Colon carcinogenesis; Cyclooxygenase 2; Grape juice; Rat