Using Bioinformatics Approach to Explore the Pharmacological Mechanisms of Multiple Ingredients in Shuang-Huang-Lian.
Due to the proved clinical efficacy, Shuang-Huang-Lian (SHL) has developed a variety of dosage forms. However, the in-depth research on targets and pharmacological mechanisms of SHL preparations was scarce. In the presented study, the bioinformatics approaches were adopted to integrate relevant data and biological information. As a result, a PPI network was built and the common topological parameters were characterized. The results suggested that the PPI network of SHL exhibited a scale-free property and modular architecture. The drug target network of SHL was structured with 21 functional modules. According to certain modules and pharmacological effects distribution, an antitumor effect and potential drug targets were predicted. A biological network which contained 26 subnetworks was constructed to elucidate the antipneumonia mechanism of SHL. We also extracted the subnetwork to explicitly display the pathway where one effective component acts on the pneumonia related targets. In conclusions, a bioinformatics approach was established for exploring the drug targets, pharmacological activity distribution, effective components of SHL, and its mechanism of antipneumonia. Above all, we identified the effective components and disclosed the mechanism of SHL from the view of system.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Bai-xia
BX
Zhang
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
Jian
J
Li
Institute of Basic Research of Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Hao
H
Gu
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Qiang
Q
Li
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Qi
Q
Zhang
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China.
Tian-jiao
TJ
Zhang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Yun
Y
Wang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Cheng-ke
CK
Cai
D019295:Computational Biology / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D047309:Flavones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D011014:Pneumonia / Q000188:drug therapy; D060066:Protein Interaction Maps / Q000187:drug effects; D047311:Luteolin
D004365:Drugs, Chinese Herbal; D047309:Flavones; D005419:Flavonoids; D005960:Glucosides; C516462:dihydrooroxylin; C516400:shuang-huang-lian; C066408:luteolin-7-glucoside; D047311:Luteolin