Anti-PRRSV effect and mechanism of tetrahydroaltersolanol C in vitro.
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important arterivirus that causes substantial economic losses to the swine industry. Current control strategies against PRRSV are still inadequate and there is an urgent need for new antiviral therapies. Tetrahydroaltersolanol C (TD-C) is a new anthraquinone derivative isolated from the marine-derived fungi. In the present study, we first demonstrated its anti-PRRSV activity in vitro through assessing the inhibition of TD-C on cytopathic effect, viral ORF7 gene and N protein expressions, progeny virions production by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, relative-quantitative RT-PCR, Western blotting, and indirect immunofluorescence assay. Our experimental results showed that TD-C could significantly inhibit PRRSV replication in a dose-dependent manner. The 50% effective concentration, 50% cytotoxic concentration and the selectivity index were 12.11, 395.31 μM, and 32.64, respectively. Furthermore, the possible anti-PRRSV mechanism was explored by virucidal assay, virus adsorption inhibition assay, and the time-of-addition assay. The results showed that TD-C might inhibit the internalization and replication of PRRSV, but did not directly inactivate the virus or block its adsorption to cell surface. In conclusion, our findings indicated that TD-C possessed a significant anti-PRRSV activity, and provided a strong basis for further exploration of this compound as an antiviral agent against PRRSV.
a Prevention Veterinary Science and Animal Biological Tecnology Key Open Laboratory , Shandong Binzhou Animal Science & Veterinary Medicine Academy , Binzhou 256600 , China.
Song-Lin
SL
Zhang
b Biology Engineering Department, Binzhou Vocational College , Binzhou 256603 , China.
Yi-Chun
YC
Wu
c Hubei Key Laboratory of Natural Products Research and Development , College of Biological and Pharmaceutical Sciences, China Three Gorges University , Yichang 443002 , China.
Fan
F
Cheng
c Hubei Key Laboratory of Natural Products Research and Development , College of Biological and Pharmaceutical Sciences, China Three Gorges University , Yichang 443002 , China.
Zhi-Yong
ZY
Guo
c Hubei Key Laboratory of Natural Products Research and Development , College of Biological and Pharmaceutical Sciences, China Three Gorges University , Yichang 443002 , China.
Jian-Feng
JF
Chen
D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology*; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D015394:Molecular Structure; D016133:Polymerase Chain Reaction; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects*; D013552:Swine; D013778:Tetrazolium Salts; D013844:Thiazoles; D014779:Virus Replication / Q000187:drug effects
D000880:Anthraquinones; D000998:Antiviral Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C000609940:tetrahydroaltersolanol C; C022616:thiazolyl blue
antiviral activity; in vitro; porcine reproductive and respiratory syndrome virus (PRRSV); tetrahydroaltersolanol C