Integrating DNA-strand-displacement circuitry with self-assembly of spherical nucleic acids.
Programmable and algorithmic behaviors of DNA molecules allow one to control the structures of DNA-assembled materials with nanometer precision and to construct complex networks with digital and analog behaviors. Here we developed a way of integrating a DNA-strand-displacement circuit with self-assembly of spherical nucleic acids, wherein a single DNA strand was used to initiate and catalyze the operation of upstream circuits to release a single strand that subsequently triggers self-assembly of spherical nucleic acids in downstream circuits, realizing a programmable kinetic control of self-assembly of spherical nucleic acids. Through utilizing this method, single-nucleotide polymorphisms or indels occurring at different positions of a sequence of oligonucleotide were unambiguously discriminated. We provide here a sophisticated way of combining the DNA-strand-displacement-based characteristic of DNA with the distinct assembly properties of inorganic nanoparticles, which may find broad potential applications in the fabrication of a wide range of complex multicomponent devices and architectures.
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Dongbao
D
Yao
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Tingjie
T
Song
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Xianbao
X
Sun
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Shiyan
S
Xiao
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Fujian
F
Huang
CAS Key Laboratory of Soft Matter Chemistry, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), Department of Polymer Science and Engineering, and ‡Hefei National Laboratory for Physical Sciences at the Microscale, National Synchrotron Radiation Laboratory, University of Science and Technology of China , Hefei, Anhui 230026, P. R. China.
Haojun
H
Liang
D000465:Algorithms; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D020641:Polymorphism, Single Nucleotide
D004277:DNA, Single-Stranded