Patterned Plasmonic Nanoparticle Arrays for Microfluidic and Multiplexed Biological Assays.
For applications ranging from medical diagnostics and drug screening to chemical and biological warfare detection, inexpensive, rapid-readout, portable devices are required. Localized surface plasmon resonance (LSPR) technologies show substantial promise toward meeting these goals, but the generation of portable, multiplexed and/or microfluidic devices incorporating sensitive nanoparticle arrays is only in its infancy. Herein, we have combined photolithography with Hole Mask Colloidal lithography to pattern uniform nanoparticle arrays for both microfluidic and multiplexed devices. The first proof-of-concept study is carried out with 5- and 7-channel microfluidic devices to acquire one-shot binding curves and protein binding kinetic data. The second proof-of-concept study involved the fabrication of a 96-spot plate that can be inserted into a standard plate reader for the multiplexed detection of protein binding. This versatile fabrication technique should prove useful in next generation chips for bioassays and genetic screening.
Department of Chemistry, College of Arts and Sciences, University of Cincinnati , 301 West Clifton Court, Cincinnati, Ohio 45221-0172, United States.
Jie
J
He
Department of Chemistry, College of Arts and Sciences, University of Cincinnati , 301 West Clifton Court, Cincinnati, Ohio 45221-0172, United States.
Michelle
M
Boegli
Department of Chemistry, College of Arts and Sciences, University of Cincinnati , 301 West Clifton Court, Cincinnati, Ohio 45221-0172, United States.
Ian
I
Bruzas
Department of Chemistry, College of Arts and Sciences, University of Cincinnati , 301 West Clifton Court, Cincinnati, Ohio 45221-0172, United States.
William
W
Lum
Department of Chemistry, College of Arts and Sciences, University of Cincinnati , 301 West Clifton Court, Cincinnati, Ohio 45221-0172, United States.
Laura
L
Sagle
D001681:Biological Assay / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties