High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.
Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.
Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands. m.wojcik@rug.nl.
Magdalena
M
Wójcik
Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands. a.telzerow@student.rug.nl.
Aline
A
Telzerow
Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands. w.j.quax@rug.nl.
Wim J
WJ
Quax
Groningen Research Institute of Pharmacy, Department of Pharmaceutical Biology, University of Groningen, A. Deusinglaan 1, 9717 AV Groningen, The Netherlands. y.l.boersma@rug.nl.
Ykelien L
YL
Boersma
D005434:Flow Cytometry; D057166:High-Throughput Screening Assays / Q000639:trends; D006801:Humans; D044085:Microfluidics / Q000639:trends*; D015202:Protein Engineering / Q000639:trends*
FACS-based screening platforms; high-throughput screening; in vitro compartmentalization; microfluidics-based screening platforms; protein engineering