Human retinal imaging using visible-light optical coherence tomography guided by scanning laser ophthalmoscopy.
We achieved human retinal imaging using visible-light optical coherence tomography (vis-OCT) guided by an integrated scanning laser ophthalmoscopy (SLO). We adapted a spectral domain OCT configuration and used a supercontinuum laser as the illumating source. The center wavelength was 564 nm and the bandwidth was 115 nm, which provided a 0.97 µm axial resolution measured in air. We characterized the sensitivity to be 86 dB with 226 µW incidence power on the pupil. We also integrated an SLO that shared the same optical path of the vis-OCT sample arm for alignment purposes. We demonstrated the retinal imaging from both systems centered at the fovea and optic nerve head with 20° × 20° and 10° × 10° field of view. We observed similar anatomical structures in vis-OCT and NIR-OCT. The contrast appeared different from vis-OCT to NIR-OCT, including slightly weaker signal from intra-retinal layers, and increased visibility and contrast of anatomical layers in the outer retina.
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston, IL, 60208 USA ; These authors contributed equally to this work.
Ji
J
Yi
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston, IL, 60208 USA ; These authors contributed equally to this work.
Siyu
S
Chen
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston, IL, 60208 USA ; These authors contributed equally to this work.
Xiao
X
Shu
Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 645 N Michigan Ave, Chicago, IL 60611 USA.
Amani A
AA
Fawzi
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston, IL, 60208 USA ; Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 645 N Michigan Ave, Chicago, IL 60611 USA.
Hao F
HF
Zhang
(110.4190) Multiple imaging; (170.0110) Imaging systems; (170.4470) Ophthalmology; (170.4500) Optical coherence tomography