Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity.
This paper describes potentiometric bioimaging for enzyme activity using a large-scale integration (LSI)-based electrochemical device with 400 sensors. Potentiometric detection is useful for bioimaging because redox species are not consumed or produced during the detection process; therefore, there is no effect on cell activity and the detectable signal is sustained. In this study, the potentiometer mode of the LSI-based device was applied for the detection of glucose oxidase (GOx) and alkaline phosphatase (ALP) activity. The enzyme activities were quantitatively detected within the concentration ranges of 25-250 μg/mL and 0.10-5.0 ng/mL. In addition, GOx activity in hydrogels and the ALP activity of embryoid bodies (EBs) from embryonic stem (ES) cells were successfully imaged based on detection of the open circuit potentials of individual sensors in real time. To the best of our knowledge, this is the first report of potentiometric imaging using LSI-based electrochemical arrays to detect enzyme activity in ES cells. The LSI-based device is thus demonstrated to be a promising tool for bioimaging of enzyme activity.
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Yusuke
Y
Kanno
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan. Electronic address: ino.kosuke@bioinfo.che.tohoku.ac.jp.
Kosuke
K
Ino
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Chika
C
Sakamoto
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Kumi Y
KY
Inoue
Micro System Integration Center, Tohoku University, 519-1176 Aramaki-aza Aoba, Aoba-ku, Sendai 980-0845, Japan.
Masahki
M
Matsudaira
Japan Aviation Electronics Industry, Ltd., 1-1 Musashino 3-chome, Akishima-shi, Tokyo 196-8555, Japan.
Atsushi
A
Suda
Japan Aviation Electronics Industry, Ltd., 1-1 Musashino 3-chome, Akishima-shi, Tokyo 196-8555, Japan.
Ryota
R
Kunikata
Micro System Integration Center, Tohoku University, 519-1176 Aramaki-aza Aoba, Aoba-ku, Sendai 980-0845, Japan; Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8579, Japan.
Tomohiro
T
Ishikawa
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Hiroya
H
Abe
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan.
Hitoshi
H
Shiku
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8579, Japan; WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan. Electronic address: matsue@bioinfo.che.tohoku.ac.jp.
Tomokazu
T
Matsue
D000818:Animals; D002478:Cells, Cultured; D003217:Conductometry / Q000295:instrumentation*; D053595:Embryonic Stem Cells / Q000201:enzymology*; D004789:Enzyme Activation; D004798:Enzymes / Q000378:metabolism*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D057054:Molecular Imaging / Q000295:instrumentation*; D011199:Potentiometry / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018511:Systems Integration
D004798:Enzymes
Bioanalysis; Chip device; Embryonic stem cells; LSI-based electrochemical sensors; Potentiometric bioimaging