Modeling the fate of antibiotic resistance genes and class 1 integrons during thermophilic anaerobic digestion of municipal wastewater solids.
This study investigated the use of thermophilic anaerobic digestion for removing antibiotic resistance genes (ARGs) from residual municipal wastewater solids. Four laboratory-scale anaerobic digesters were operated in 8-day batch cycles at temperatures of 40, 56, 60, and 63 °C. Two tetracycline resistance genes (tet(W) and tet(X)), a fluoroquinolone resistance gene (qnrA), the integrase gene of class 1 integrons (intI1), 16S rRNA genes of all Bacteria, and 16S rRNA genes of methanogens were quantified using real-time quantitative PCR. ARG and intI1 quantities decreased at all temperatures and were described well by a modified form of the Collins-Selleck disinfection kinetic model. The magnitudes of Collins-Selleck kinetic parameters were significantly greater at thermophilic temperatures compared to 40 °C, but few statistically significant differences were observed among these parameters for the thermophilic anaerobic digesters. This model allows for the direct comparison of different operating conditions (e.g., temperature) on anaerobic digestion performance in mitigating the quantity of ARGs in wastewater solids and could be used to design full-scale anaerobic digesters to specifically treat for ARGs as a "pollutant" of concern.
Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, 500 Pillsbury Drive SE, Minneapolis, MN, 55455, USA.
Tucker R
TR
Burch
Biotechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA.|Department of Soil, Water, and Climate, University of Minnesota, St. Paul, MN, 55108, USA.
Michael J
MJ
Sadowsky
Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, 500 Pillsbury Drive SE, Minneapolis, MN, 55455, USA. lapar001@umn.edu.|Biotechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA. lapar001@umn.edu.
Timothy M
TM
LaPara
Anaerobic digestion; Antibiotic resistance genes; Class 1 integrons; Collins-Selleck model; Tetracycline resistance; Thermophilic