Cytocompatibility of the selected calcium phosphate based bone cements: comparative study in human cell culture.
Calcium phosphate cements (CPC) are valuable bone fillers. Recently they have been also considered as the basis for drug-, growth factors- or cells-delivery systems. Broad possibilities to manipulate CPC composition provide a unique opportunity to obtain materials with a wide range of physicochemical properties. In this study we show that CPC composition significantly influences cell response. Human bone derived cells were exposed to the several well-characterized different cements based on calcium phosphates, magnesium phosphates and calcium sulfate hemihydrate (CSH). Cell viability assays, live/dead staining and real-time observation of cells in contact with the materials (time-laps) were performed. Although all the investigated materials have successfully passed a standard cytocompatibility assay, cell behavior in a direct contact with the materials varied depending on the material and the experimental system. The most recommended were the α-TCP-based materials which proved suitable as a support for cells in a direct contact. The materials which caused a decrease of calcium ions concentration in culture induced the negative cell response, however this effect might be expected efficiently compensated in vivo. All the materials consisting of CSH had negative impact on the cells. The obtained results strongly support running series of cytocompatibility studies for preclinical evaluation of bone cements.
Department of Histology and Embryology, Centre for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.|Department of Pathology, Medical University of Warsaw, Warsaw, Poland.|Centre for Preclinical Research and Technology, Medical University of Warsaw, Warsaw, Poland.
Radosław
R
Olkowski
Department of Histology and Embryology, Centre for Biostructure Research, Medical University of Warsaw, Warsaw, Poland.|Centre for Preclinical Research and Technology, Medical University of Warsaw, Warsaw, Poland.
Piotr
P
Kaszczewski
Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Kraków, Poland.
Joanna
J
Czechowska
Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Kraków, Poland.
Dominika
D
Siek
Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Kraków, Poland.
Dawid
D
Pijocha
Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Kraków, Poland.
Aneta
A
Zima
Faculty of Materials Science and Ceramics, AGH-University of Science and Technology, Kraków, Poland.
Anna
A
Ślósarczyk
Department of Histology and Embryology, Centre for Biostructure Research, Medical University of Warsaw, Warsaw, Poland. mszumiel@wum.edu.pl.|Centre for Preclinical Research and Technology, Medical University of Warsaw, Warsaw, Poland. mszumiel@wum.edu.pl.
Małgorzata
M
Lewandowska-Szumieł
D001843:Bone Cements; D002130:Calcium Phosphates; D002478:Cells, Cultured; D006801:Humans; D014961:X-Ray Diffraction
D001843:Bone Cements; D002130:Calcium Phosphates; C020243:calcium phosphate