TCEA3 Attenuates Gastric Cancer Growth by Apoptosis Induction.
BACKGROUND
The aim of this study was to investigate and interpret the expression level and potential function of TCEA3 in gastric cancer.


METHODS
qRT-PCR was used to determine the expression level of TCEA3 in gastric cancer tissues. Pearson χ2 test was performed to clarify the correlation between TCEA3 expression and patients' clinicopathologic characteristics. Biological function of TCEA3 was tested by proliferation assay and colony formation assay. Flow cytometry was used to study the potential function of TCEA3 in apoptosis induction.


RESULTS
TCEA3 expression was significantly downregulated in gastric cancer tissues compared with paired normal tissues. Poor prognoses were observed in the low TCEA3 expression group of patients in contrast to the high TCEA3 expression group. Functionally, upregulation of TCEA3 inhibited gastric cancer cell proliferation and colony formation. We also found that TCEA3 may attenuate cell growth through apoptosis induction.


CONCLUSIONS
Our findings suggest that TCEA3 attenuates the proliferation and induces apoptosis of gastric cancer cells.
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Jia
J
Li
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Yin
Y
Jin
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Shuang
S
Pan
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Yina
Y
Chen
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Kaijing
K
Wang
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Chunjing
C
Lin
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Shuqing
S
Jin
Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China (mainland).
Jiansheng
J
Wu
D000368:Aged; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D035602:Transcriptional Elongation Factors / Q000378:metabolism*
D018076:DNA, Complementary; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II