Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications.
First, the potential role of Raman-based techniques in biomedicine is introduced. Second, an overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus of recent developments. Third, imaging strategies are summarized including sequential registration with laser scanning microscopes, line imaging and global or wide-field imaging. Finally, examples of biomedical applications are presented in the context of single cells, laser tweezers, tissue sections, biopsies and whole animals.
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany. juergen.popp@ipht-jena.de.
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Krafft
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany. juergen.popp@ipht-jena.de.
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Schie
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany.
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Meyer
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany.
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Schmitt
Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany. juergen.popp@ipht-jena.de and Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743 Jena, Germany.
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Popp
D000818:Animals; D035843:Biomedical Research; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D008853:Microscopy / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*