New insights about ORF1 coding regions support the proposition of a new genus comprising arthropod viruses in the family Totiviridae.
Analyzing the positions of 2A-like polypeptide cleavage sites in all available genomes of arthropod totiviruses we propose the limits of all ORF1 coding sequences and observed that two proteins previously predicted in infectious myonecrosis virus genome are unique in the arthropod totiviruses group. A putative protein cleavage site upstream the major capsid protein was also identified only in these genomes. In addition, protein models generated using ab initio and threading approaches revealed conserved structures possibly related to formation of viral protrusions and RNA packaging, clarifying the mechanisms involved in the extracellular transmission. These data appoints that the group formed by arthropod totiviruses are sufficient distinctive to be clustered in new genus belonging to the Totiviridae family, in agreement with previous phylogenetic analysis.
Laboratório de Biologia Molecular Aplicada, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Márcia Danielle A
MD
Dantas
Laboratório de Biologia Molecular Aplicada, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Gustavo Henrique O
GH
Cavalcante
Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
Raffael A C
RA
Oliveira
Laboratório de Biologia Molecular Aplicada, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil. Electronic address: danielclanza@gmail.com.
Daniel C F
DC
Lanza
D000595:Amino Acid Sequence; D000818:Animals; D001181:Arthropods / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D019196:Totiviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D014764:Viral Proteins
Armigeres subalbatus virus; Double strand RNA viruses; Drosophila melanogaster virus; Giardia lamblia virus; Infectious myonecrosis virus