Identification, modeling, and characterization studies of Tetrahymena thermophila myosin FERM domains suggests a conserved core fold but functional differences.
Myosins (MYO) define a superfamily of motor proteins which facilitate movement along cytoskeletal actin filaments in an ATP-dependent manner. To date, over 30 classes of myosin have been defined that vary in their roles and distribution across different taxa. The multidomain tail of myosin is responsible for the observed functional differences in different myosin classes facilitating differential binding to different cargos. One domain found in this region, the FERM domain, is found in several diverse proteins and is involved in many biological functions ranging from cell adhesion and actin-driven cytoskeleton assembly to cell signaling. Recently, new classes of unconventional myosin have been identified in Tetrahymena thermophila. In this study, we have identified, modeled, and characterized eight FERM domains from the unconventional T. thermophila myosins as their complete functional MyTH4-FERM cassettes. Our results reveal notable sequence, structural, and electrostatic differences between T. thermophila and other characterized FERM domains. Specifically, T. thermophila FERM domains contain helical inserts or extensions, which contribute to significant differences in surface electrostatic profiles of T. thermophila myosin FERMs when compared to the conventional FERM domains. Analyses of the modeled domains reveal differences in key functional residues as well as phosphoinositide-binding signatures and affinities. The work presented here broadens the scope of our understanding of myosin classes and their inherent functions, and provides a platform for experimentalists to design rational experimental studies to test the functional roles for T. thermophila myosins.
Biology Department, the Graduate Center, City University of New York, 365 Fifth Avenue, New York, New York, 10016.
Che L
CL
Martin
Biology Department, the Graduate Center, City University of New York, 365 Fifth Avenue, New York, New York, 10016.|Department of Biology, Brooklyn College of the City University of New York, 2900 Bedford Ave. Brooklyn, New York, 11210, USA.
Shaneen M
SM
Singh
D003599:Cytoskeleton / Q000378:metabolism*; D008958:Models, Molecular; D009218:Myosins / Q000378:metabolism*; D011485:Protein Binding; D016808:Tetrahymena thermophila / Q000378:metabolism*
D009218:Myosins
FERM; Tetrahymena thermophila; modeling; motor proteins; myosin