Luteolin Ameliorates Hypertensive Vascular Remodeling through Inhibiting the Proliferation and Migration of Vascular Smooth Muscle Cells.
Objectives. Preliminary researches showed that luteolin was used to treat hypertension. However, it is still unclear whether luteolin has effect on the hypertensive complication such as vascular remodeling. The present study was designed to investigate the effect of luteolin on the hypertensive vascular remodeling and its molecular mechanism. Method and Results. We evaluated the effect of luteolin on aorta thickening of hypertension in spontaneous hypertensive rats (SHRs) and found that luteolin could significantly decrease the blood pressure and media thickness of aorta in vivo. Luteolin could inhibit angiotensin II- (Ang II-) induced proliferation and migration of vascular smooth muscle cells (VSMCs). Dichlorofluorescein diacetate (DCFH-DA) staining result showed that luteolin reduced Ang II-stimulated ROS production in VSMCs. Furthermore, western blot and gelatin zymography results showed that luteolin treatment leaded to a decrease in ERK1/2, p-ERK1/2, p-p38, MMP2, and proliferating cell nuclear antigen (PCNA) protein level. Conclusion. These data support that luteolin can ameliorate hypertensive vascular remodeling by inhibiting the proliferation and migration of Ang II-induced VSMCs. Its mechanism is mediated by the regulation of MAPK signaling pathway and the production of ROS.
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Jie
J
Su
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Han-Ting
HT
Xu
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Jing-Jing
JJ
Yu
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Jian-Li
JL
Gao
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Jing
J
Lei
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Qiao-Shan
QS
Yin
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Bo
B
Li
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Min-Xia
MX
Pang
Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Min-Xia
MX
Su
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Wen-Jia
WJ
Mi
Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.
Su-Hong
SH
Chen
Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China ; Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
Gui-Yuan
GY
Lv