Characterization of a S-adenosyl-l-methionine (SAM)-accumulating strain of Scheffersomyces stipitis.
S-adenosyl-l-methionine (SAM) is an important molecule in the cellular metabolism of mammals. In this study, we examined several of the physiological characteristics of a SAM-accumulating strain of the yeast Scheffersomyces stipitis (M12), including SAM production, ergosterol content, and ethanol tolerance. S. stipitis M12 accumulated up to 52.48 mg SAM/g dry cell weight. Proteome analyses showed that the disruption of C-24 methylation in ergosterol biosynthesis, a step mediated by C-24 sterol methyltransferase (Erg6p), results in greater SAM accumulation by S. stipitis M12 compared to the wild-type strain. A comparative proteome-wide analysis identified 25 proteins that were differentially expressed by S. stipitis M12. These proteins are involved in ribosome biogenesis, translation, the stress response, ubiquitin-dependent catabolic processes, the cell cycle, ethanol tolerance, posttranslational modification, peroxisomal membrane stability, epigenetic regulation, the actin cytoskeleton and cell morphology, iron and copper homeostasis, cell signaling, and energy metabolism.
Laboratory for Fermentation and Yeast Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Stela
S
Križanović
Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Ana
A
Butorac
Laboratory for Fermentation and Yeast Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Jasna
J
Mrvčić
Laboratory for Fermentation and Yeast Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Maja
M
Krpan
Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, Ruder Bošković Institute, Zagreb, Croatia.
Mario
M
Cindrić
Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Višnja
V
Bačun-Družina
Laboratory for Fermentation and Yeast Technology, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Damir
D
Stanzer
D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D012436:S-Adenosylmethionine / Q000096:biosynthesis* / Q000737:chemistry; D004718:Saccharomycetales / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D005656:Fungal Proteins; D000431:Ethanol; D012436:S-Adenosylmethionine
C-24 sterol methyltransferase (Erg6p); S-adenosyl- l-methionine (SAM); SAM accumulating yeast; Scheffersomyces stipitis