Changes in different parameters, lymphocyte proliferation and hematopoietic progenitor colony formation in EAE mice treated with myelin oligodendrocyte glycoprotein.
Myelin oligodendrocyte glycoprotein (MOG) is an antigen of the myelin sheath, which may trigger immune cell responses and the production of auto-antibodies in multiple sclerosis (MS). In this study, we used MOG(35-55) -induced experimental autoimmune encephalomyelitis (EAE), a model of human MS, to assess the production of catalytically active immunoglobulin G (IgG) antibodies or abzymes which have been shown to be present in sera of patients with several autoimmune diseases. Here, we show that IgGs from the sera of control C57BL/6 mice are catalytically inactive. During development of EAE, a specific reorganization of the immune system of mice occurred leading to a condition which was associated with the generation of catalytically active IgGs hydrolysing DNA, myelin basic protein (MBP) and MOG which was associated with increased proteinuria, changes in differentiation of mice bone marrow hematopoietic stem cells (HSCs) and an increase in proliferation of lymphocytes in bone marrow, spleen and thymus as well as a significant suppression of cell apoptosis in these organs. The strongest alterations were found in the early disease phase (18-24 days after immunization) and were less pronounced in later EAE stages (40 days after EAE induction). We conclude that a significant increase in DNase and proteolytic activities of antibodies may be considered the earliest statistically significant marker of MOG-induced EAE in mice. The possible differences in immune system reorganizations during preclinical phases of the disease, acute and late EAE, leading to production of different auto-antibodies and abzymes as well other changes are discussed.
Novosibirsk Medical University, Ministry of Public Health of Russian Federation, Novosibirsk, Russia.
Vasilii B
VB
Doronin
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Taisiya A
TA
Parkhomenko
Institute Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Alexey
A
Korablev
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Ludmila B
LB
Toporkova
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Julia A
JA
Lopatnikova
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Alina A
AA
Alshevskaja
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Sergei V
SV
Sennikov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russia.
Valentina N
VN
Buneva
Institut für Physiologie I, Westfälische Wilhelms-Universität, Münster, Germany.
Thomas
T
Budde
Department of Neurology, Westfälische Wilhelms-Universität, Münster, Germany.
Sven G
SG
Meuth
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.
Irina A
IA
Orlovskaya
Institute Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russia.
Nelly A
NA
Popova
Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, Russia.|Novosibirsk State University, Novosibirsk, Russia.
Georgy A
GA
Nevinsky
D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*
D063308:Myelin-Oligodendrocyte Glycoprotein
EAE model, C57BL/6 mice; apoptosis in different organs; catalytic antibodies; colony formation; hematopoietic progenitors; lymphocyte proliferation