COMPOSITE POLYMER-COATED MINERAL SCAFFOLDS FOR BONE REGENERATION: FROM MATERIAL CHARACTERIZATION TO HUMAN STUDIES.
Bovine bone xenografts, made of hydroxyapatite (HA), were coated with poly(L-lactide-co-ε- caprolactone) (PLCL) and RGD-containing collagen fragments in order to increase mechanical properties, hydrophilicity, cell adhesion and osteogenicity. In vitro the scaffold microstructure was investigated with Environmental Scanning Electronic Microscopy (ESEM) analysis and micro tomography, while mechanical properties were investigated by means compression tests. In addition, cell attachment and growth within the three-dimensional scaffold inner structure were validated using human osteosarcoma cell lines (SAOS-2 and MG-63). Standard ISO in vivo biocompatibility studies were carried out on model animals, while bone regenerations in humans were performed to assess the efficacy of the product. All results from in vitro to in vivo investigations are here reported, underlining that this scaffold promotes bone regeneration and has good clinical outcome.
Industrie Biomediche Insubri SA, Mezzovico-Vira, Switzerland.
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Pertici
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
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Carinci
Private practice, Ponsacco, Italy.
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Carusi
Universitatea de Medicina si Farmacie Carol Davila, Bucharest, Romania.
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Epistatus
Politecnico di Milano, Laboratory of Biological Structure Mechanics, Department of Chemistry, Materials and Chemical Engineering G. Natta, Milan, Italy; IRCCS Istituto Ortopedico Galeazzi, Milan, ltaly.
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Villa
Azienda Ospedaliera di Circolo, Busto Arsizio, Italy.
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Crivelli
Politecnico di Milano, Physical Chemistry Laboratory, Department of Chemistry, Materials and Chemical Engineering G. Natta, Milan, Italy.
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Rossi
Industrie Biomediche Insubri SA, Mezzovico-Vira, Switzerland; Department of innovative Technologies, University for Applied Science and Art of Southern Switzerland, Manno, Switzerland.
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Perale