Micro-flow Immunosensor Based on Thin-film Interdigitated Gold Array Microelectrodes for Cancer Biomarker Detection.
In this paper, we reported the development of a micro-flow label-free impedimetric biosensor based on the use of thin-film interdigitated gold array microelectrodes (IDA) for the detection of carbohydrate antigen 125 (CA125). The immunosensor is developed through the electropolymerization of anthranilic acid (AA) on the surface of IDA electrodes followed by the covalent attachment of anti-CA125 monoclonal antibody. CA125 protein affinity reaction was then evaluated by means of electrochemical impedance spectroscopy (EIS). The sensor was characterized by electrochemical techniques and scanning electron microscopy (SEM). Using the optimized experimental conditions, the developed immunosensor showed a good analytical performance for CA125 detection from 0 to 100 U/mL with estimated limit of detection (LOD = 3Sblank/Slope) of 7 U/mL.
Andrea
A
Ravalli
Luca
L
Lozzi
Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy. giovanna.marrazza@unifi.it.
Giovanna
G
Marrazza
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000032:analysis* / Q000276:immunology; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning; D058105:Polymerization; D062367:ortho-Aminobenzoates / Q000737:chemistry
D000906:Antibodies; D018394:CA-125 Antigen; C451759:MUC16 protein, human; D008565:Membrane Proteins; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D006046:Gold