Comparative effects of diet and carcinogen on microRNA expression in the stem cell niche of the mouse colonic crypt.
There is mounting evidence that noncoding microRNAs (miRNA) are modulated by select chemoprotective dietary agents. For example, recently we demonstrated that the unique combination of dietary fish oil (containing n-3 fatty acids) plus pectin (fermented to butyrate in the colon) (FPA) up-regulates a subset of putative tumor suppressor miRNAs in intestinal mucosa, and down-regulates their predicted target genes following carcinogen exposure as compared to control (corn oil plus cellulose (CCA)) diet. To further elucidate the biological effects of diet and carcinogen modulated miR's in the colon, we verified that miR-26b and miR-203 directly target PDE4B and TCF4, respectively. Since perturbations in adult stem cell dynamics are generally believed to represent an early step in colon tumorigenesis and to better understand how the colonic stem cell population responds to environmental factors such as diet and carcinogen, we additionally determined the effects of the chemoprotective FPA diet on miRNAs and mRNAs in colonic stem cells obtained from Lgr5-EGFP-IRES-creER(T2) knock-in mice. Following global miRNA profiling, 26 miRNAs (P<0.05) were differentially expressed in Lgr5(high) stem cells as compared to Lgr5(negative) differentiated cells. FPA treatment up-regulated miR-19b, miR-26b and miR-203 expression as compared to CCA specifically in Lgr5(high) cells. In contrast, in Lgr5(negative) cells, only miR-19b and its indirect target PTK2B were modulated by the FPA diet. These data indicate for the first time that select dietary cues can impact stem cell regulatory networks, in part, by modulating the steady-state levels of miRNAs. To our knowledge, this is the first study to utilize Lgr5(+) reporter mice to determine the impact of diet and carcinogen on miRNA expression in colonic stem cells and their progeny.
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Intercollegiate Faculty of Genetics, Texas A&M University, College Station, TX, United States; Divison of Endocrinology, Boston Children's Hospital, United States; Department of Pediatrics, Harvard Medical School, Boston, MA, United States.
Manasvi S
MS
Shah
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Departments of Molecular and Cellular Medicine, Texas A&M University, College Station, TX, United States.
Eunjoo
E
Kim
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States.
Laurie A
LA
Davidson
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Electrical Engineering, Texas A&M University, College Station, TX, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
Jason M
JM
Knight
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Statistics, Texas A&M University, College Station, TX, United States.
Roger S
RS
Zoh
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
Jennifer S
JS
Goldsby
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States.
Evelyn S
ES
Callaway
Veterinary Physiology & Pharmacology, Texas A&M University, College Station, TX, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
Beyian
B
Zhou
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Veterinary Physiology & Pharmacology, Texas A&M University, College Station, TX, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States.
Ivan
I
Ivanov
Program in Integrative Nutrition & Complex Diseases, Texas A&M University, College Station, TX, United States; Intercollegiate Faculty of Genetics, Texas A&M University, College Station, TX, United States; Center for Translational Environmental Health Research, Texas A&M University, College Station, TX, United States. Electronic address: r-chapkin@tamu.edu.
Robert S
RS
Chapkin
D000818:Animals; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000235:genetics; D004032:Diet; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D051418:Focal Adhesion Kinase 2 / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D065840:Protective Factors; D055153:Stem Cell Niche / Q000187:drug effects; D000073940:Transcription Factor 4 / Q000235:genetics
D002273:Carcinogens; D015525:Fatty Acids, Omega-3; D035683:MicroRNAs; D000073940:Transcription Factor 4; D051418:Focal Adhesion Kinase 2; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4
Chemoprevention; Colon cancer; Colon stem cells; Diet; Lgr5; MicroRNA