MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis.
Fat-induced transcript 1 (FIT1/FITM1) gene is a member of the conserved gene family important for triglyceride-rich lipid droplet accumulation. FIT1 gene displays a similar muscle-specific expression across pigs, mice, and humans. Thus pigs can act as a useful model of many human diseases resulting from misexpression of FIT1 gene. Triglyceride content in skeletal muscle plays a key role in pork meat quality and flavors. An insertion/deletion mutation in porcine FIT1 coding region shows a high correlation with a series of fat traits. To gain better knowledge of the potential role of FIT1 gene in human diseases and the correlations with pork meat quality, our attention is given to the region upstream of the porcine FIT1 coding sequence. We cloned ~1 kb of the 5'-flanking region of porcine FIT1 gene to define the role of this sequence in modulating the myogenic expression. A canonical E-box element that activated porcine FIT1 promoter activity during myogenesis was identified. Further analysis demonstrated that promoter activity was induced by overexpression of MyoD1, which bound to this canonical E-box during C2C12 differentiation. This is the first evidence that FIT1 as the direct novel target of MyoD is involved in muscle development.
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. yanchi_2005@126.com.
Chi
C
Yan
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. xxl8365@163.com.
Xiaoliang
X
Xia
Yuguan Agricultural Inc., Shuining 629208, China. hejunxian2011@yahoo.com.
Junxian
J
He
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. renzq@mail.hzau.edu.cn.
Zhuqing
Z
Ren
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. dequanxu@mail.hzau.edu.cn.
Dequan
D
Xu
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. yzhxiong@126.com.
Yuanzhu
Y
Xiong
Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China. zuobo@mail.hzau.edu.cn.
Bo
B
Zuo
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D024721:E-Box Elements / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development / Q000235:genetics / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D015533:Transcriptional Activation
D008565:Membrane Proteins; D017570:MyoD Protein
C2C12 myotube; ChIP; E-box; MyoD1; muscle; porcine FIT1 gene regulation