Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds.
The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. The resulting hybrid materials at [AgNPs] < 0.4 μM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 μM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.
Division of Cardiac Surgery, University of Ottawa Heart Institute, Ottawa, Ontario, CanadaK1Y 4W7.
Emilio I
EI
Alarcon
Klas I
KI
Udekwu
Christopher W
CW
Noel
Luke B-P
LB
Gagnon
Patrick K
PK
Taylor
Branka
B
Vulesevic
Madeline J
MJ
Simpson
Spyridon
S
Gkotzis
M Mirazul
MM
Islam
Chyan-Jang
CJ
Lee
Agneta
A
Richter-Dahlfors
Thien-Fah
TF
Mah
Erik J
EJ
Suuronen
Juan C
JC
Scaiano
May
M
Griffith
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development; D054423:Chemokine CCL24 / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
D000890:Anti-Infective Agents; C515485:Ccl24 protein, mouse; D054423:Chemokine CCL24; D020100:Hydrogels; D018836:Inflammation Mediators; D015850:Interleukin-6; D047889:Receptors, Tumor Necrosis Factor, Type II; C560825:Timp1 protein, mouse; D019715:Tissue Inhibitor of Metalloproteinase-1; D012834:Silver