miR-19a and SOCS-1 expression in the differential diagnosis of laryngeal (glottic) verrucous squamous cell carcinoma.
BACKGROUND
Laryngeal verrucous squamous cell carcinoma (VSCC) is a highly differentiated squamous cell carcinoma (SCC), the diagnosis of which can meet with many pitfalls: benign hyperplastic lesions and conventional SCC are the most important differential diagnoses. The microRNA miR-19a is overexpressed in many solid tumours and regulates the suppressor of cytokine signalling-1 (SOCS-1) expression.


OBJECTIVE
The main endpoints were to assess miR-19a and SOCS-1 expression in glottic VSCC, and the former's potential role in differentiating between glottic VSCC, conventional SCC and hyperplastic lesions.


METHODS
The expression of MiR-19a (by reverse transcription and quantitative real-time PCR) and SOCS-1 (by immunohistochemistry, rabbit polyclonal anti-SOCS-1 antibody) was assessed in 11 consecutive cases of glottic VSCC, 20 of papillary hyperplasia and 42 cases of conventional SCC.


RESULTS
Mean miR-19a expression was significantly higher (p = 0.000) in malignant glottic lesions (conventional SCC/VSCC) than in benign conditions. Significant differences in mean miR-19a expression also emerged between conventional SCC and papillary hyperplasia (p = 0.000), and between conventional SCC and VSCC (p = 0.03). miR-19a expression was not statistically associated with SOCS-1 immunoreactivity or immunostaining intensity in VSCC, conventional SCC or papillary hyperplasia.


CONCLUSIONS
Our preliminary outcomes suggest the utility of miR-19a in the challenging differential diagnosis of laryngeal VSCC. Although miR-19a has been found to regulate SOCS-1 expression, this evidence was not confirmed by this investigation.
Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
Gino
G
Marioni
Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy Istituto di Ricerca Pediatrica-Città della Speranza, Padova, Italy Nanomedicine Department, The Methodist Hospital Research Institute, Houston, Texas, USA.
Marco
M
Agostini
Department of Medicine DIMED, University of Padova, Padova, Italy.
Rocco
R
Cappellesso
Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy Istituto di Ricerca Pediatrica-Città della Speranza, Padova, Italy.
Chiara
C
Bedin
Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
Giancarlo
G
Ottaviano
Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
Rosario
R
Marchese-Ragona
Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
Andrea
A
Lovato
Department of Neurosciences DNS, Otolaryngology Section, Padova University, Padova, Italy.
Tommaso
T
Cacco
Department of Medicine DIMED, University of Padova, Padova, Italy.
Luciano
L
Giacomelli
Department of Surgical, Oncological and Gastroenterological Sciences, Padova University, Padova, Italy.
Donato
D
Nitti
Department of Medicine DIMED, University of Padova, Padova, Italy.
Stella
S
Blandamura
Department of Neurosciences DNS, Odontostomatology Institute, Padova University, Padova, Italy.
Edoardo
E
Stellini
Department of Neurosciences DNS, Audiology Unit, Treviso Hospital, Padova University, Treviso, Italy.
Cosimo
C
de Filippis
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D007830:Larynx / Q000378:metabolism / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
C549214:MIRN19A microRNA, human; D035683:MicroRNAs; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins
CANCER; LARYNGEAL CANCER; TUMOUR MARKERS