Proteomics Analysis to Identify and Characterize the Molecular Signatures of Hepatic Steatosis in Ovariectomized Rats as a Model of Postmenopausal Status.
Postmenopausal women are particularly at increased risk of developing non-alcoholic fatty liver disease (NAFLD). Here we aimed to determine the impact of postmenopausal-induced NAFLD (PM-NAFLD) in an ovariectomized rat model. Sixteen six-week-old Sprague-Dawley female rats were randomly divided into two groups (eight per group), for sham-operation (Sham) or bilateral ovariectomy (Ovx). Four months after surgery, indices of liver damage and liver histomorphometry were measured. Both serum aspartate aminotransferase (AST) and alanine aminotranferease (ALT) levels were significantly higher in the Ovx than Sham group. We performed quantitative LC-MS/MS-based proteomic profiling of livers from rats with PM-NAFLD to provide baseline knowledge of the PM-NAFLD proteome and to investigate proteins involved in PM-NAFLD by ingenuity pathways analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Of the 586 identified proteins, the levels of 59 (10.0%) and 48 (8.2%) were significantly higher and lower, respectively, in the Ovx group compared to the Sham group. In conclusion, the changes in regulation of proteins implicated in PM-NAFLD may affect other vital biological processes in the body apart from causing postmenopause-mediated liver dysfunction. Our quantitative proteomics analysis may also suggest potential biomarkers and further clinical applications for PM-NAFLD.
Proteomics Research Center, National Yang-Ming University, Taipei 11221, Taiwan. ccliao@ym.edu.tw.
Chen-Chung
CC
Liao
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. 1021301@ntsu.edu.tw.|Department of Orthopedic Surgery, Taipei Medical University Shuang Ho Hospital, New Taipei City 23561, Taiwan. 1021301@ntsu.edu.tw.|School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan. 1021301@ntsu.edu.tw.
Yen-Shuo
YS
Chiu
School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan. wanchun@tmu.edu.tw.
Wan-Chun
WC
Chiu
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. peggytung@ntsu.edu.tw.
Yu-Tang
YT
Tung
National Laboratory Animal Center, National Applied Research Laboratories, Taipei 11529, Taiwan. p650214@nlac.narl.org.tw.
Hsiao-Li
HL
Chuang
Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan. eric@nchu.edu.tw.
Jyh-Horng
JH
Wu
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan. john5523@ntsu.edu.tw.
Chi-Chang
CC
Huang
D000818:Animals; D002853:Chromatography, Liquid; D005260:Female; D008099:Liver / Q000201:enzymology / Q000473:pathology*; D065626:Non-alcoholic Fatty Liver Disease / Q000378:metabolism*; D010052:Ovariectomy; D017698:Postmenopause / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
D020543:Proteome
ingenuity pathways analysis; liver; proteome