[The Impact of Electroacupuncture Intervention on Expression of 5-HTR 1 B/2 C Genes in Mice under Radiation Stimulation from Mobile Phone].
OBJECTIVE
To observe the effect of electroacupuncture (EA) stimulation of "Yifen" (TE 17), "Shenshu" (BL 23) on the expression of 5-hydroxytryptamine receptor 1 B (5-HTR 1 B) mRNA and 5-hydroxytryptamine receptor 2 C (5-HTR 2 C) mRNA in the cochlear nucleus tissue in mice experiencing radiation from mobile phone, so as to explore its mechanisms underlying improvement of tinnitus.


METHODS
Thirty Kunming mice were randomly divided into control group (n = 6) and modeling group (n = 24). The tinnitus model was established by giving the mice with mobile phone-radiation for 1 h in the morning and 1 h in the afternoon, continuously for 40 days. EA stimulation was applied to "Yifeng" (TE 17) group (n = 6) and "Shenshu" (BL 23) group (n = 6) for 20 min, once a day for 7 days. The expression of 5-THR 1 B/2 C mRNA in the cochlear nucleus was assayed by fluorescence quantitative polymerase chain reaction (real time-PCR).


RESULTS
The expression level of 5-HTR 1 B was significantly lower in the model group than in the control group (P < 0.05), while that of 5-HTR 2 C mRNA significantly increased (P < 0.01). TE 17 group received a significant acupoint intervention effect (P < 0.01). Compared with TE 17 group, BL 23 group received a weaker effect (P < 0.05).


CONCLUSIONS
EA of TE 17 can up-regulate expression level of 5-HTR 1 B and down-regulate expression level of 5-HTR 2 C in the cochlear nucleus in mice experiencing mobile-phone radiation.
Jian-yu
JY
Dai
Yi-guo
YG
Chen
Xiao-qing
XQ
Zhang
D015669:Acupuncture Points; D000818:Animals; D040421:Cell Phone; D003051:Cochlea / Q000378:metabolism / Q000528:radiation effects*; D004427:Ear Diseases / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D015671:Electroacupuncture; D005260:Female; D006801:Humans; D051379:Mice; D011846:Radio Waves / Q000009:adverse effects*; D044302:Receptor, Serotonin, 5-HT1B / Q000235:genetics* / Q000378:metabolism; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism
C000599853:5-hydroxytryptamine2C receptor, mouse; C000603631:HTR1B protein, mouse; D044302:Receptor, Serotonin, 5-HT1B; D044405:Receptor, Serotonin, 5-HT2C; D012701:Serotonin