Effects of the Dietary Addition of Amaranth (Amaranthus mantegazzianus) Protein Isolate on Antioxidant Status, Lipid Profiles and Blood Pressure of Rats.
The effects of the dietary addition of 2.5% (w/w) Amaranthus mantegazzianus protein isolate (AI) on blood pressure, lipid profiles and antioxidative status of Wistar rats were evaluated. Six diets were used to feed animals during 28 days: (base (AIN93G), Chol (cholesterol 1%, w/w), CE (α-tocopherol 0.005%, w/w), CholE (cholesterol 1% (w/w) + α-tocopherol 0.005%, w/w), CAI (AI 2.5% w/w), CholAI (cholesterol 1% (w/w) + AI 2.5%, w/w). Lipid profiles of plasma and liver and faecal cholesterol content were analyzed. Antioxidant status was evaluated by the ferric reducing activity of plasma (FRAP), the 2-thiobarbituric acid (TBA) assay and superoxide dismutase (SOD) activity in plasma and liver. Blood pressure was measured in the tail artery of rats. CholA group presented a significant (α < 0.05) reduction (16%) in the plasma total cholesterol. In liver, the intake of cholesterol (Chol group) induced a significant increment in cholesterol and triglycerides (2.5 and 2.3 times, respectively), which could be decreased (18% and 47%, respectively) by the addition of AI (CholA group). This last group also showed an increased faecal cholesterol excretion (20%). Increment (50%) in FRAP values, diminution of TBA value in plasma and liver (70% and 38%, respectively) and diminution of SOD activity (20%) in plasma of CholA group suggest an antioxidant effect because of the intake of AI. In addition, CA and CholA groups presented a diminution (18%) of blood pressure after 28 days.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) - CCT La Plata-CONICET, UNLP, 47 y 116 (1900), La Plata, Argentina.
María B
MB
Lado
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) - CCT La Plata-CONICET, UNLP, 47 y 116 (1900), La Plata, Argentina.
Julieta
J
Burini
Centro de Investigaciones Cardiovasculares - CCT La Plata-CONICET, Facultad de Medicina (UNLP), 60 y 120 (1900), La Plata, Argentina.
Gustavo
G
Rinaldi
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) - CCT La Plata-CONICET, UNLP, 47 y 116 (1900), La Plata, Argentina.
María C
MC
Añón
Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) - CCT La Plata-CONICET, UNLP, 47 y 116 (1900), La Plata, Argentina. vtironi@quimica.unlp.edu.ar.
Valeria A
VA
Tironi
D027721:Amaranthus / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D001794:Blood Pressure / Q000187:drug effects*; D002784:Cholesterol / Q000032:analysis / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004032:Diet; D005243:Feces / Q000737:chemistry; D008055:Lipids / Q000032:analysis* / Q000097:blood; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008297:Male; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D024502:alpha-Tocopherol / Q000008:administration & dosage
D000975:Antioxidants; D002791:Cholesterol, Dietary; D008055:Lipids; D010940:Plant Proteins; D014280:Triglycerides; D002784:Cholesterol; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol
Amaranth protein isolate; Antioxidant status; Hypolipidemic profile; Hypotensive effect; Wistar rats