Design of Enzyme-Encapsulated Protein Containers by In Vivo Crystal Engineering.
Crystalline protein assemblies of polyhedra crystal (PhC) can be utilized as solid enzyme containers for long-term storage of enzymes with retention of their enzymatic activity. The enzymes can be released from the crystals at the optimum pH for the enzymatic activity by dissolution of the crystals using in vivo crystal engineering.
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
Satoshi
S
Abe
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
Hiroshi
H
Ijiri
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
Hashiru
H
Negishi
Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Hiroyuki
H
Yamanaka
Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Katsuhito
K
Sasaki
SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, 1-1-1, Kouto, Sayo-cho, Sayo-gun, Hyogo, 679-5148, Japan.
Kunio
K
Hirata
Center for Advanced Insect Research Promotion, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Hajime
H
Mori
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho, 4259-B55, Midori-ku, Yokohama, 226-8501, Japan.
Takafumi
T
Ueno
D000818:Animals; D002214:Capsules; D003460:Crystallization; D008958:Models, Molecular; D015202:Protein Engineering / Q000379:methods*; D011493:Protein Kinase C / Q000235:genetics*; D017433:Protein Structure, Secondary; D061987:Sf9 Cells; D018411:Spodoptera; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics*
D002214:Capsules; D015678:Viral Structural Proteins; D011493:Protein Kinase C
crystal engineering; enzyme encapsulation; polyhedra; protein crystals