Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol-polycaprolactone copolymer solutions.
Amphiphilic block copolymer methoxy polyethyleneglycol-polycaprolactone (mPEG-PCL) has attracted interest in the biomedical field, due to its water solubility and biodegradability. Nevertheless, the blood safety of mPEG-PCL copolymers has not been investigated in detail. Because mPEG-PCL copolymers introduced in vivo would inevitably interact with blood tissue, an investigation of possible interactions of mPEG-PCL with key blood components is crucial. We studied the effects of two mPEG-PCL copolymer solutions on blood coagulation, the morphology and lysis of human red blood cells (RBCs), the structure of plasma fibrinogen, complement activation, and platelet aggregation. We found that higher concentrations of the mPEG-PCL copolymers impaired blood clotting, and the copolymers had little impact on the morphology or lysis of RBCs. From the spectroscopy results, the copolymers affected the local microstructure of fibrinogen. The copolymers significantly activated the complement system in a concentration-dependent way. At higher concentrations, the copolymers impaired platelet aggregation, which may have been mediated by an inhibition of the arachidonic acid pathway. These findings provide important information that may be useful for the molecular design and biomedical applications of mPEG-PCL copolymers. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 802-812, 2016.
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, People's Republic of China.
Qian
Q
Hu
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, People's Republic of China.
Yi
Y
Zhang
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, People's Republic of China.|Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, People's Republic of China.
Changyong
C
Wang
The School of Pathology and Laboratory Medicine, the University of Western Australia, Perth, Australia.
Jiake
J
Xu
3D Imaging and Bioengineering, the Department of Mechanical Engineering, Curtin University, Perth, Australia.
Jianping
J
Wu
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, People's Republic of China.|Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, People's Republic of China.
Zonghua
Z
Liu
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, People's Republic of China.|Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, Jinan University, Guangzhou, People's Republic of China.
Wei
W
Xue
D000244:Adenosine Diphosphate / Q000494:pharmacology; D016718:Arachidonic Acid / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002942:Circular Dichroism; D003167:Complement Activation / Q000187:drug effects; D015926:Complement C3a / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D018056:Hemorheology / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008422:Materials Testing / Q000379:methods*; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation / Q000187:drug effects; D011091:Polyesters / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011517:Prothrombin Time; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013916:Thrombelastography; D013929:Thromboxane B2 / Q000378:metabolism
D011091:Polyesters; D012996:Solutions; C439611:methoxy poly(ethylene glycol-co-epsilon-caprolactone); D016718:Arachidonic Acid; D011092:Polyethylene Glycols; D013929:Thromboxane B2; D000244:Adenosine Diphosphate; D015926:Complement C3a; D005340:Fibrinogen
coagulation; hemocompatibility; mPEG-PCL; platelet