Quantitative Detection Method of Hydroxyapatite Nanoparticles Based on Eu(3+) Fluorescent Labeling in Vitro and in Vivo.
One major challenge for application of hydroxyapatite nanoparticles (nHAP) in nanomedicine is the quantitative detection method. Herein, we exploited one quantitative detection method for nHAP based on the Eu(3+) fluorescent labeling via a simple chemical coprecipitation method. The trace amount of nHAP in cells and tissues can be quantitatively detected on the basis of the fluorescent quantitative determination of Eu(3+) ions in nHAP crystal lattice. The lowest concentration of Eu(3+) ions that can be quantitatively detected is 0.5 nM using DELFIA enhancement solution. This methodology can be broadly applicable for studying the tissue distribution and metabolization of nHAP in vivo.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology , Wuhan 430070, P.R. China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology , Wuhan 430070, P.R. China.
Yunfei
Y
Xie
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology , Wuhan 430070, P.R. China.|Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka , 70140 Belihuloya, Sri Lanka.
Thalagalage Shalika Harshani
TS
Perera
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology , Wuhan 430070, P.R. China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology , Wuhan 430070, P.R. China.
Fang
F
Li
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology , Wuhan 430070, P.R. China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology , Wuhan 430070, P.R. China.
Yingchao
Y
Han
School of Medicine, Hubei Institute of Technology , Huangshi, Hubei 435003, China.
Meizhen
M
Yin
D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D046529:Microscopy, Electron, Transmission; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution; D014961:X-Ray Diffraction
D005456:Fluorescent Dyes; D007477:Ions; D012996:Solutions; D005063:Europium; D017886:Durapatite
Eu3+ ions; fluorescent labeling; hydroxyapatite; nanoparticles; quantitative detection