Adaptation of a Cell-Based High Content Screening System for the In-Depth Analysis of Celiac Biopsy Tissue.
The IN Cell Analyzer 1000 possesses several distinguishing features that make it a valuable tool in research today. This fully automated high content screening (HCS) system introduced quantitative fluorescent microscopy with computerized image analysis for use in cell-based analysis. Previous studies have focused on live cell assays, where it has proven to be a powerful and robust method capable of providing reproducible, quantitative data. Using HCS as a tool to investigate antigen expression in duodenal biopsies, we developed a novel approach to tissue positioning and mapping. We adapted IN Cell Analyzer 1000's image acquisition and analysis software for the investigation of tissue transglutaminase (tTG) and smooth muscle alpha-actin (SM α-actin) staining in paraffin-embedded duodenal tissue sections from celiac patients and healthy controls. These innovations allowed a quantitative analysis of cellular structure and protein expression. The results from routine biopsy material indicated the intensity of protein expression was altered in celiac disease compared to normal biopsy material.
Immunology Department, TCD, Dublin, Ireland. cooperse@tcd.ie.
Sarah E J
SE
Cooper
Immunology Department, TCD, Dublin, Ireland. bmohamed@tcd.ie.|Thoracic Oncology, St James Hospital, Dublin, Ireland. bmohamed@tcd.ie.
Bashir M
BM
Mohamed
Immunology Department, TCD, Dublin, Ireland. loelliott@tcd.ie.
Louise
L
Elliott
INCHSA, Institute for Molecular Medicine, TCD, Dublin, Ireland. Anthony.Davies@tcd.ie.
Anthony Mitchell
AM
Davies
Immunology Department, TCD, Dublin, Ireland. con.feighery@tcd.ie.|Immunology Department, St James Hospital, Dublin, Ireland. con.feighery@tcd.ie.
Conleth F
CF
Feighery
National Children's Research Centre, Our Lady's Children's Hospital, Dublin, Ireland. jacinta.kelly@ncrc.ie.
Jacinta
J
Kelly
Immunology Department, TCD, Dublin, Ireland. dunnej1@tcd.ie.|Immunology Department, St James Hospital, Dublin, Ireland. dunnej1@tcd.ie.
Jean
J
Dunne
D001706:Biopsy; D002446:Celiac Disease / Q000473:pathology*; D006801:Humans; D008856:Microscopy, Fluorescence
Celiac disease (CD); High content screening (HCS); Smooth muscle alpha-actin (SM α-actin); Tissue transglutaminase (tTG)