A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies.
Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA +/AMR-) patients were compared to both DSA-positive, AMR-positive (DSA +/AMR +) patients as well as DSA-negative (DSA -) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR - patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084.
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA.
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Ó Broin
Division of Pediatric Nephrology, University of Texas Health Science Center, Houston, TX, USA.
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Hayde
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA.
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Y
Bao
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA.
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B
Ye
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA.
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Calder
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA ; Division of Nephrology, Albert Einstein College of Medicine, Bronx, NY, USA.
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de Boccardo
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA ; Division of Nephrology, Albert Einstein College of Medicine, Bronx, NY, USA.
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Lubetzky
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA ; Division of Nephrology, Albert Einstein College of Medicine, Bronx, NY, USA.
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Ajaimy
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA.
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J
Pullman
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA.
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A
Colovai
Montefiore-Einstein Center for Transplantation, Montefiore Medical Center, The University Hospital for Albert Einstein College of Medicine, Bronx, NY, USA.
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E
Akalin
Department of Genetics, Albert Einstein College of Medicine, Bronx, NY, USA ; Department of Mathematical Sciences, Yeshiva University, New York, NY, USA.
A
A
Golden
Antibody-mediated rejection; Donor-specific antibodies; Gene expression; Kidney; Transplant