High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration.
Screening of high affinity and high specificity peptide probes towards various targets is important in the biomedical field while traditional peptide screening procedure is manual and tedious. Herein, a bimodal imprinting microarray system to embrace the whole peptide screening process is presented. Surface Plasmon Resonance imaging (SPRi) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) are combined for both quantitative and qualitative identification of the peptide. The method provides a solution for high efficiency peptide screening.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, No.11. Beiyitiao Zhongguancun, Beijing, 100190, China.
Weizhi
W
Wang
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, No.11. Beiyitiao Zhongguancun, Beijing, 100190, China. fangqj@nanoctr.cn.
Qiaojun
Q
Fang
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, No.11. Beiyitiao Zhongguancun, Beijing, 100190, China. huzy@nanoctr.cn.
Zhiyuan
Z
Hu
D000595:Amino Acid Sequence; D019921:Biotinylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D060327:Solid-Phase Synthesis Techniques; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*; D018511:Systems Integration
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D010455:Peptides
Bimodal imprinting chip; MALDI-TOF-MS; Microarray; OBOC; SPRi