Computational Identification of MicroRNAs and Their Targets from Finger Millet (Eleusine coracana).
MicroRNAs are endogenous small RNAs regulating intrinsic normal growth and development of plant. Discovering miRNAs, their targets and further inferring their functions had become routine process to comprehend the normal biological processes of miRNAs and their roles in plant development. In this study, we used homology-based analysis with available expressed sequence tag of finger millet (Eleusine coracana) to predict conserved miRNAs. Three potent miRNAs targeting 88 genes were identified. The newly identified miRNAs were found to be homologous with miR166 and miR1310. The targets recognized were transcription factors and enzymes, and GO analysis showed these miRNAs played varied roles in gene regulation. The identification of miRNAs and their targets is anticipated to hasten the pace of key epigenetic regulators in plant development.
Maharani's Science College for Women, Palace road, Bangalore, 560001, India.|Faculty of Biological Engineering, Shobhit University, Meerut, UP, 250110, India.
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Usha
Maharani's Science College for Women, Palace road, Bangalore, 560001, India.|Faculty of Biological Engineering, Shobhit University, Meerut, UP, 250110, India.
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Jyothi
Maharani's Science College for Women, Palace road, Bangalore, 560001, India.
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Suchithra
Faculty of Biological Engineering, Shobhit University, Meerut, UP, 250110, India.
Rekha
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Dixit
Faculty of Biological Engineering, Shobhit University, Meerut, UP, 250110, India.
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Rai
Maharani's Science College for Women, Palace road, Bangalore, 560001, India. nageshbabur@gmail.com.
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Nagesh Babu
D031748:Eleusine / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D035683:MicroRNAs; D014157:Transcription Factors
EST; GO analysis; MYB; Transcription factors