Impact of the DNA polymerase Theta on the DNA replication program.
The physiological function of the human DNA polymerase θ (pol θ) is still unclear despite its in vitro translesion synthesis capacity during DNA damage repair process. However this DNA polymerase is always present along the cell cycle in the absence of replication stress and DNA damage. Is there a different molecular function? We present the genomic data of replication timing in depleted pol θ cells (GSE49693) and in cells overexpressing pol θ (GSE53070) indicating that Pol θ holds a novel role in the absence of external stress as a critical determinant of the replication timing program in human cells.
Institut Jacques Monod, UMR7592, CNRS and University Paris-Diderot, 15 Rue Hélène Brion, Paris, Cedex 13 75205, France.
Giuseppe
G
Baldacci
CRCT (Cancer Research Center of Toulouse), INSERM Unit 1037, CHU Purpan, Toulouse 31024, France ; CNRS ERL 5294 BP3028, BP3028, CHU Purpan, Toulouse 31024, France ; CRCT (Cancer Research Center of Toulouse), BP3028, CHU Purpan, Toulouse 31024, France.
Jean-Sebastien
JS
Hoffmann
Institut Jacques Monod, UMR7592, CNRS and University Paris-Diderot, 15 Rue Hélène Brion, Paris, Cedex 13 75205, France.
Jean-Charles
JC
Cadoret
DNA polymerase theta; DNA replication timing; Microarray