Effects of Dietary Plant Sterols and Stanol Esters with Low- and High-Fat Diets in Chronic and Acute Models for Experimental Colitis.
In this study, we evaluated the effects of dietary plant sterols and stanols as their fatty acid esters on the development of experimental colitis. The effects were studied both in high- and low-fat diet conditions in two models, one acute and another chronic model of experimental colitis that resembles gene expression in human inflammatory bowel disease (IBD). In the first experiments in the high fat diet (HFD), we did not observe a beneficial effect of the addition of plant sterols and stanols on the development of acute dextran sulphate sodium (DSS) colitis. In the chronic CD4CD45RB T cell transfer colitis model, we mainly observed an effect of the presence of high fat on the development of colitis. In this HFD condition, the presence of plant sterol or stanol did not result in any additional effect. In the second experiments with low fat, we could clearly observe a beneficial effect of the addition of plant sterols on colitis parameters in the T cell transfer model, but not in the DSS model. This positive effect was related to the gender of the mice and on Treg presence in the colon. This suggests that especially dietary plant sterol esters may improve intestinal inflammation in a T cell dependent manner.
Tytgat Institute for Liver and Intestinal Research, Amsterdam Medical Centre, Amsterdam 1105 BK, The Netherlands. a.a.tevelde@amc.nl.
Anje A
AA
te Velde
Department of Human Biology or Pediatrics, School for Nutrition, Toxicology and Metabolism, Maastricht University, Maastricht 6229 ER, The Netherlands. florence.brull@maastrichtuniversity.nl.
Florence
F
Brüll
Tytgat Institute for Liver and Intestinal Research, Amsterdam Medical Centre, Amsterdam 1105 BK, The Netherlands. s.e.heinsbroek@amc.uva.nl.
Sigrid E M
SE
Heinsbroek
Department of Pathology, Amsterdam Medical Centre, Amsterdam 1105 AZ, The Netherlands. s.l.meijer@amc.uva.nl.
Sybren L
SL
Meijer
Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, Bonn D-53127, Germany. Dieter.Luetjohann@ukb.uni-bonn.de.
Dieter
D
Lütjohann
Department of Human Biology or Pediatrics, School for Nutrition, Toxicology and Metabolism, Maastricht University, Maastricht 6229 ER, The Netherlands. a.vreugdenhil@mumc.nl.
Anita
A
Vreugdenhil
Department of Human Biology or Pediatrics, School for Nutrition, Toxicology and Metabolism, Maastricht University, Maastricht 6229 ER, The Netherlands. j.plat@maastrichtuniversity.nl.
Jogchum
J
Plat
D000208:Acute Disease; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D015703:Antigens, CD; D029741:Brassica rapa / Q000737:chemistry; D002908:Chronic Disease; D003092:Colitis / Q000178:diet therapy* / Q000188:drug therapy / Q000209:etiology / Q000276:immunology; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D004952:Esters; D005227:Fatty Acids / Q000494:pharmacology / Q000627:therapeutic use; D005229:Fatty Acids, Monounsaturated; D005260:Female; D007249:Inflammation / Q000178:diet therapy / Q000188:drug therapy / Q000276:immunology; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D008297:Male; D008810:Mice, Inbred C57BL; D010840:Phytosterols / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010938:Plant Oils / Q000737:chemistry; D000074262:Rapeseed Oil; D012855:Sitosterols / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D015703:Antigens, CD; D004041:Dietary Fats; D004952:Esters; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D010840:Phytosterols; D010938:Plant Oils; D000074262:Rapeseed Oil; D012855:Sitosterols
T cells; experimental colitis; inflammation; nutrition; plant stanols; plant sterols