Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches as well as high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.
Department of Biology, Reed College, Portland, OR, USA.
Derek A
DA
Applewhite
Department of Biology, University of Richmond, B-214 Gottwald science Center, 28 Westhampton Way, Richmond, VA, 23173, USA.
Christine A
CA
Davis
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, UK.
Eric R
ER
Griffis
Department of Biology, University of Richmond, B-214 Gottwald science Center, 28 Westhampton Way, Richmond, VA, 23173, USA. oquinter@richmond.edu.
Omar A
OA
Quintero
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival; D003599:Cytoskeleton / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011107:Polylysine / Q000494:pharmacology; D013194:Staining and Labeling; D013499:Surface Properties; D014162:Transfection
D011107:Polylysine
Actin; Electron microscopy; Fluorescence microscopy; Microtubule; S2 cells; Transfection