Assessment of terbium (III) as a luminescent probe for the detection of tuberculosis biomarkers.
A detection method for nicotinic acid, a specific metabolite marker of Mycobacterium tuberculosis present in cultures and patients' breath, is studied in complex solutions containing other metabolites and in biological media such as urine, saliva and breath condensate. The method is based on the analysis of the luminescence increase of Tb(3+) complexes in the presence of nicotinic acid due to the energy transfer from the excited ligand to the lanthanide ion. It is shown that other potential markers found in M. tuberculosis culture supernatant, such as methyl phenylacetate, p-methyl anisate, methyl nicotinate and 2-methoxy biphenyl, can interfere with nicotinic acid via a competitive absorption of the excitation photons. A new strategy to circumvent these interferences is proposed with an upstream trapping of volatile markers preceding the detection of nicotinic acid in the liquid phase via the luminescence of Tb(3+) complexes. The cost of the method is evaluated and compared with the Xpert MTB/RIF test endorsed by the World Health Organization.
CNRS, IRAMIS, UMR 3685 NIMBE/LEDNA, F-91191 Gif-sur-Yvette, France.
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Bamogo
CEA, IRAMIS, UMR 3685 NIMBE/LEDNA, F-91191 Gif-sur-Yvette, France.
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L
Mugherli
CNRS, IRAMIS, LIDyL/Laboratoire Francis Perrin, URA 2453, F-91191 Gif-sur-Yvette, France.
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A
Banyasz
BioMérieux SA, F-38000 Grenoble, France.
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Novelli-Rousseau
BioMérieux SA, F-38000 Grenoble, France.
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Mallard
CNRS, IRAMIS, UMR 3685 NIMBE/LEDNA, F-91191 Gif-sur-Yvette, France. Electronic address: thu-hoa.tran-thi@cea.fr.
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Tran-Thi
D015415:Biomarkers / Q000032:analysis; D006801:Humans; D049408:Luminescent Agents / Q000032:analysis / Q000737:chemistry*; D009169:Mycobacterium tuberculosis / Q000737:chemistry* / Q000378:metabolism; D009525:Niacin / Q000032:analysis*; D009942:Organometallic Compounds / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*
D015415:Biomarkers; D049408:Luminescent Agents; D009942:Organometallic Compounds; D013725:Terbium; D009525:Niacin
Breath condensate; Metabolite markers; Mycobacterium tuberculosis; Nicotinic acid; Terbium complexes; Time-resolved luminescence