Secondary Structure Determination of Peptides and Proteins After Immobilization.
The presentation of immobilized peptides and other small biomolecules attached to surfaces can be greatly affected by the attachment chemistry and linking moieties, resulting in altered activity and specificity. For this reason, it is critical to understand how the various aspects of surface immobilization-underlying substrate properties, tether structure, and site of linkage-affect the secondary and quaternary structures of the immobilized species. Here, we present methods for attaching cysteine-containing peptides to quartz surfaces and determining the secondary structure of surface-immobilized peptides. We specifically show that, even when covalently immobilized, changes in peptide conformation can still occur, with measurement occurring in real time.
US Naval Research Laboratory, Code 6900, 4555 Overlook Avenue, SW, Washington, DC, 20375, USA. Stella.north@nrl.navy.mil.|Latham & Watkins LLP, 555 Eleventh Street, NW, Washington, DC, 20004, USA. Stella.north@nrl.navy.mil.
Stella H
SH
North
US Naval Research Laboratory, Code 6900, 4555 Overlook Avenue, SW, Washington, DC, 20375, USA. chris.taitt@nrl.navy.mil.
Chris R
CR
Taitt
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002942:Circular Dichroism / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D055767:Immobilized Proteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary / Q000187:drug effects; D011791:Quartz / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D023181:Antimicrobial Cationic Peptides; D003902:Detergents; D055767:Immobilized Proteins; D011791:Quartz; D012822:Silicon Dioxide; C031161:cecropin A
Antimicrobial peptide; Bioimmobilization; CD spectroscopy; Circular dichroism; Peptide structure; Quaternary structure; Secondary structure