Effect of rhamnolipid biosurfactant on solubilization of polycyclic aromatic hydrocarbons.
Rhamnolipid biosurfactant-producing bacteria, Bacillus Lz-2, was isolated from oil polluted water collected from Dongying Shengli oilfield, China. The factors that influence PAH solubilization such as biosurfactant concentration, pH, ionic strength and temperature were discussed. The results showed that the solubilities of naphthalene, phenanthrene and pyrene increased linearly with the rise of rhamnolipid biosurfactant dose above the biosurfactant critical micelle concentration (CMC). Furthermore, the molar solubilization ratio (MSR) values decreased in the following order: naphthalene>phenanthrene>pyrene. However, the solubility percentage increased and followed the opposite order: pyrene>phenanthrene>naphthalene. The solubilities of PAHs in rhamnolipid biosurfactant solution increased with the rise of pH and ionic strength, and reached the maximum values under the conditions of pH11 and NaCl concentration 8 g · L(-1). The solubility of phenanthrene and pyrene increased with the rise of temperature.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Shudong
S
Li
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Yongrui
Y
Pi
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mtbao@ouc.edu.cn.
Mutai
M
Bao
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Cong
C
Zhang
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Dongwei
D
Zhao
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Yiming
Y
Li
Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology, State Oceanic Administration, Qingdao 266033, China; North China Sea Environmental Monitoring Center of State Oceanic Administration, Qingdao 266033, China.
Peiyan
P
Sun
College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Jinren
J
Lu
D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism; D002681:China; D006017:Glycolipids / Q000737:chemistry*; D008823:Micelles; D009281:Naphthalenes / Q000032:analysis / Q000737:chemistry; D009994:Osmolar Concentration; D059392:Petroleum Pollution / Q000032:analysis; D010616:Phenanthrenes / Q000032:analysis / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry*; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry*
D006017:Glycolipids; D008823:Micelles; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical; C418382:rhamnolipid; C031721:naphthalene; C031181:phenanthrene; C030984:pyrene
Naphthalene; Phenanthrene; Polycyclic aromatic hydrocarbons (PAHs); Pyrene; Rhamnolipid biosurfactant; Solubilization