Identification and characterization of a mitochondrial unfolded protein response transcription factor ATFS-1 in Litopenaeus vannamei.
A mitochondrial specific stress response termed mitochondrial unfolded protein response (UPR(mt)) is activated in responding to disturbance of protein homeostasis in mitochondria. The activating transcription factor associated with stress-1 (designated as ATFS-1) is the key regulator of UPR(mt). To investigating the roles of ATFS-1 (LvATFS-1) in Litopenaeus vannamei mitochondrial stress remission and immunity, it's full length cDNA was cloned. The open reading frame of LvATFS-1 was 1, 557 bp in length, deducing to a 268 amino acids protein. LvATFS-1 was highly expressed in muscle, hemocytes and eyestalk. Subcellular location assays showed that N-terminal of LvATFS-1 contained a mitochondrial targeting sequence, which could directed the fused EGFP located to mitochondria. And the C-terminal of LvATFS-1, which had a nuclear localization signal, expressed in nucleus. The in vitro experiments verified that LvATFS-1 could reduced the level of intracellular reactive oxygen species (ROS). And results of real-time RT-PCR indicated that LvATFS-1 might scavenge excess ROS via ROS-eliminating genes regulation. Reporter gene assays showed that LvATFS-1 could upregulated the expression of the antimicrobial peptide genes in Drosophila Schneider 2 cells. Results of real-time RT-PCR showed that Vibrio alginolyticus or white spot syndrome virus (WSSV) infection induced the expression of LvATFS-1. And knocked-down LvATFS-1 by RNAi resulted in a higher cumulative mortality of L. vannamei upon V. alginolyticus or WSSV infection. These results suggested that LvATFS-1 not only rolled in mitochondrial specific stress responding, but also important for L. vannamei immunologic defence.
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Yong-Gui
YG
Chen
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Hai-Tao
HT
Yue
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Ze-Zhi
ZZ
Zhang
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Feng-Hua
FH
Yuan
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Hai-Tao
HT
Bi
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Kai
K
Yuan
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Shao-Ping
SP
Weng
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
Jian-Guo
JG
He
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China. Electronic address: chenyh6@mail.sysu.edu.cn.
Yi-Hong
YH
Chen
D051696:Activating Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D056811:Unfolded Protein Response; D044149:Vibrio alginolyticus / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
D051696:Activating Transcription Factors; D017382:Reactive Oxygen Species
ATFS-1; Innate immunity; Litopenaeus vannamei; Mitochondrial unfolded protein response; ROS