Abscisic Acid Stimulates Glucagon-Like Peptide-1 Secretion from L-Cells and Its Oral Administration Increases Plasma Glucagon-Like Peptide-1 Levels in Rats.
In recent years, Abscisic Acid (ABA) has been demonstrated to be involved in the regulation of glucose homeostasis in mammals as an endogenous hormone, by stimulating both insulin release and peripheral glucose uptake. In addition, ABA is released by glucose- or GLP-1-stimulated β-pancreatic cells. Here we investigated whether ABA can stimulate GLP-1 release. The human enteroendocrine L cell line hNCI-H716 was used to explore whether ABA stimulates in vitro GLP-1 secretion and/or transcription. ABA induced GLP-1 release in hNCI-H716 cells, through a cAMP/PKA-dependent mechanism. ABA also enhanced GLP-1 transcription. In addition, oral administration of ABA significantly increased plasma GLP-1 and insulin levels in rats. In conclusion, ABA can stimulate GLP-1 release: this result and the previous observation that GLP-1 stimulates ABA release from β -cells, suggest a positive feed-back mechanism between ABA and GLP-1, regulating glucose homeostasis. Type 2 diabetes treatments targeting the GLP-1 axis by either inhibiting its rapid clearance by dipeptidyl-peptidase IV or using GLP-1 mimetics are currently used. Moreover, the development of treatments aimed at stimulating GLP-1 release from L cells has been considered as an alternative approach. Accordingly, our finding that ABA increases GLP-1 release in vitro and in vivo may suggest ABA and/or ABA analogs as potential anti-diabetic treatments.
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Santina
S
Bruzzone
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Mirko
M
Magnone
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Elena
E
Mannino
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Giovanna
G
Sociali
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Laura
L
Sturla
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Chiara
C
Fresia
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Valeria
V
Booz
Animal facility, IRCCS AOU San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Laura
L
Emionite
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Antonio
A
De Flora
Department of Experimental Medicine (DIMES), Section of Biochemistry, and CEBR, University of Genova, Genova, Italy.
Elena
E
Zocchi
D000040:Abscisic Acid / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects*; D045744:Cell Line, Tumor; D000242:Cyclic AMP / Q000378:metabolism; D019858:Enteroendocrine Cells / Q000378:metabolism; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood*; D008565:Membrane Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D028044:Phosphate-Binding Proteins; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; C471704:LANCL2 protein, human; D008565:Membrane Proteins; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D000040:Abscisic Acid; D052216:Glucagon-Like Peptide 1; D000242:Cyclic AMP