High-Throughput Microarray Incubations Using Multi-Well Chambers.
Peptide microarrays are ideal tools for a variety of applications ranging from epitope mapping to immune monitoring. Here we present a method for high-throughput screening of biological samples using only standard microtiter plate equipment. Parallel incubation of a large number of samples with a small library of peptides is enabled by printing multiple identical mini-arrays on one microarray slide and further combining four slides to yield an incubation frame possessing the dimensions of a 96-well microtiter plate. Applying conventional lab equipment such as ELISA washers, hundreds of samples can be processed in 1 day yielding approx. 200 data points in triplicates per sample.
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany. zerweck@jpt.com.
Johannes
J
Zerweck
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Ulf
U
Reimer
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Janina
J
Jansong
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Nikolaus
N
Pawlowski
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Christoph
C
Tersch
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Maren
M
Eckey
JPT Peptide Technologies GmbH, Volmerstr. 5, 12489, Berlin, Germany.
Tobias
T
Knaute
D000595:Amino Acid Sequence; D053000:Analytic Sample Preparation Methods; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*
D010455:Peptides
Biomarker verification; High-throughput screening; Multi-well incubation chamber; Peptide microarray