Biofunctionalization of Polyoxometalates with DNA Primers, Their Use in the Polymerase Chain Reaction (PCR) and Electrochemical Detection of PCR Products.
The bioconjugation of polyoxometalates (POMs), which are inorganic metal oxido clusters, to DNA strands to obtain functional labeled DNA primers and their potential use in electrochemical detection have been investigated. Activated monooxoacylated polyoxotungstates [SiW11 O39 {Sn(CH2 )2 CO}](8-) and [P2 W17 O61 {Sn(CH2 )2 CO}](6-) have been used to link to a 5'-NH2 terminated 21-mer DNA forward primer through amide coupling. The functionalized primer was characterized by using a battery of techniques, including electrophoresis, mass spectrometry, as well as IR and Raman spectroscopy. The functionality of the POM-labeled primers was demonstrated through hybridization with a surface-immobilized probe. Finally, the labeled primers were successfully used in the polymerase chain reaction (PCR) and the PCR products were characterized by using electrophoresis.
Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona (Spain).
Ahmed M
AM
Debela
Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona (Spain).
Mayreli
M
Ortiz
Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona (Spain).
Valerio
V
Beni
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire (UMR 8232), 4 place Jussieu, 75005 Paris (France).
Serge
S
Thorimbert
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire (UMR 8232), 4 place Jussieu, 75005 Paris (France).
Denis
D
Lesage
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire (UMR 8232), 4 place Jussieu, 75005 Paris (France).
Richard B
RB
Cole
Departament d'Enginyeria Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona (Spain). ciara.osullivan@urv.cat.|ICREA, Passeig Lluis Companys 23, 08010 Barcelona (Spain). ciara.osullivan@urv.cat.
Ciara K
CK
O'Sullivan
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire (UMR 8232), 4 place Jussieu, 75005 Paris (France). bernold.hasenknopf@upmc.fr.
Bernold
B
Hasenknopf
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017973:Tungsten Compounds / Q000737:chemistry*; D000099294:Polyoxometalates
D004247:DNA; D017931:DNA Primers; D017973:Tungsten Compounds; C102296:polyoxometalate I; D000099294:Polyoxometalates
DNA; electrochemistry; polymerase chain reaction; polyoxometalates; redox chemistry