Crystal structure of mature 2S albumin from Moringa oleifera seeds.
2S albumins, the seed storage proteins, are the primary sources of carbon and nitrogen and are involved in plant defense. The mature form of Moringa oleifera (M. oleifera), a chitin binding protein isoform 3-1 (mMo-CBP3-1) a thermostable antifungal, antibacterial, flocculating 2S albumin is widely used for the treatment of water and is potentially interesting for the development of both antifungal drugs and transgenic crops. The crystal structure of mMo-CBP3-1 determined at 1.7 Å resolution demonstrated that it is comprised of two proteolytically processed α-helical chains, stabilized by four disulfide bridges that is stable, resistant to pH changes and has a melting temperature (TM) of approximately 98 °C. The surface arginines and the polyglutamine motif are the key structural factors for the observed flocculating, antibacterial and antifungal activities. This represents the first crystal structure of a 2S albumin and the model of the pro-protein indicates the structural changes that occur upon formation of mMo-CBP3-1 and determines the structural motif and charge distribution patterns for the diverse observed activities.
Multiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil. Electronic address: anwar.ms90@yahoo.com.
Anwar
A
Ullah
Multiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil.
Ricardo Barros
RB
Mariutti
Multiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil.
Rehana
R
Masood
Multiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil.
Icaro Putinhon
IP
Caruso
Departamento de Tecnologia, FCAV/UNESP, Campus de Jaboticabal, Brazil.
Gustavo Henrique Gravatim
GH
Costa
Departamento de Tecnologia, FCAV/UNESP, Campus de Jaboticabal, Brazil.
Cristhyane
C
Millena de Freita
Brazilian Biosciences National Laboratory (LNBio), National Center for Research in Energy and Materials, Campinas, SP, 13083-970, Brazil.
Camila Ramos
CR
Santos
Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, SP, Brazil.
Leticia Maria
LM
Zanphorlin
Departamento de Tecnologia, FCAV/UNESP, Campus de Jaboticabal, Brazil.
Márcia Justino
MJ
Rossini Mutton
Brazilian Biosciences National Laboratory (LNBio), National Center for Research in Energy and Materials, Campinas, SP, 13083-970, Brazil.
Mario Tyago
MT
Murakami
Multiuser Center for Biomolecular Innovation, Department of Physics, IBILCE/UNESP, São Jose do Rio Preto, SP, Brazil. Electronic address: arni@sjrp.unesp.br.
Raghuvir Krishnaswamy
RK
Arni
D055516:2S Albumins, Plant / Q000737:chemistry*; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D029588:Moringa oleifera / Q000737:chemistry*; D011487:Protein Conformation; D012639:Seeds / Q000737:chemistry*
D055516:2S Albumins, Plant
2S albumin; Crystal structure; Flocculating activity; Moringa oleifera seeds