Flavobacterium jejuensis sp. nov., isolated from marine brown alga Ecklonia cava.
A bacterial strain, designated EC11(T) was isolated from brown alga Ecklonia cava collected from Jeju Island, Korea. EC11(T) was identified as a Gram-negative, rod-shaped and yellow-pigmented bacterial strain. The strain EC11(T) grew over a temperature range of 10 °C to 30 °C (optimally at 25 °C), and a pH range of 6.0-10.5 (optimally at pH 7.5). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EC11(T) belongs to the genus Flavobacterium. Strain EC11(T) shared close similarity with Flavobacterium jumunjinense HME7102(T) (96.4%), Flavobacterium dongtanense LW30(T) (95.8%), Flavobacterium haoranii LQY-7(T) (95.3%), and Flavobacterium urocaniciphilum (95.1%). The major fatty acids (> 5%) were iso-C17:0 3-OH (22.4%), iso-C15:0 3-OH (19.0%), C15:0 (12.4%), summed feature 3 (comprising C16:1 ω7c/ C16:1 ω6c; 9.78%), iso-C15:1 G (9.6%), and iso-C16:0 3-OH (9.0%). The DNA G+C content was 28.1 mol% and the strain contained MK-6 as the predominant menaquinone. The major polar lipids were phosphatidylethanolamine, two unknown aminolipids and three unknown polar lipids. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain EC11T represents a novel species of the Flavobacterium genus, for which the name Flavobacterium jejuensis sp. nov. is proposed. The type strain of F. jejuensis is EC11(T) (=KCTC 42149(T) = JCM 30735(T)).
Department of Aquatic Life Medicine, College of Ocean Science, Jeju National University, Jeju, 690-756, Republic of Korea.
So-Hyun
SH
Park
Research Institute for Basic Science, Jeju National University, Jeju, 690-756, Republic of Korea.|Jeju Biological Resource Co., Ltd, CTC Business Incubator, Jeju Tourism College, Jeju, 690-756, Republic of Korea.
Ji-Young
JY
Kim
Jeju Biological Resource Co., Ltd, CTC Business Incubator, Jeju Tourism College, Jeju, 690-756, Republic of Korea. jym1006@jejunu.ac.kr.
Young-Ju
YJ
Kim
Department of Aquatic Life Medicine, College of Ocean Science, Jeju National University, Jeju, 690-756, Republic of Korea. msheo@jejunu.ac.kr.
Moon-Soo
MS
Heo
D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000737:chemistry; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D000459:Phaeophyceae / Q000382:microbiology*; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6
16S rRNA gene; Ecklonia cava; Flavobacterium; brown alga