Oxidation resistance 1 (OXR1) participates in silkworm defense against bacterial infection through the JNK pathway.
Bacterial infection causes enhanced reactive oxygen species (ROS) levels in insects. Oxidation resistance 1 (OXR1) plays an antioxidant role in eukaryotic organisms, including insects. In this report, we demonstrated that Pseudomonas aeruginosa and Staphylococcus aureus infection and hydrogen peroxide (H2O2) injection induced the expression of specific transcriptional isoforms of OXR1 in larval silkworms. We further showed that a Jun kinase (JNK) pathway inhibitor, SP600125, down-regulated expression of OXR1 during infection, leading to elevated H2O2levels in the hemolymph, resulting in lower viability of the injected bacteria inside the silkworm larvae. Our study suggests that OXR1 participates in protecting larval silkworms from oxidative stress and bacterial infection through the JNK pathway.
Department of Entomology, College of Plant Protection.
Li-De
LD
Su
Department of Entomology, College of Plant Protection.
Qiao-Ling
QL
Zhang
Department of Entomology, College of Plant Protection.|Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A&F University, Yangling, Shaanxi 712100, China.
Zhiqiang
Z
Lu
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D012831:Bombyx / Q000235:genetics* / Q000382:microbiology*; D006458:Hemolymph / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000382:microbiology; D020935:MAP Kinase Signaling System; D011550:Pseudomonas aeruginosa / Q000502:physiology; D013211:Staphylococcus aureus / Q000502:physiology
D000873:Anthracenes; C432165:pyrazolanthrone; D006861:Hydrogen Peroxide; D048031:JNK Mitogen-Activated Protein Kinases
Bombyx mori; Jun kinase pathway; infection; oxidation resistance 1; reactive oxygen species