Mapping of genomic double-strand breaks by ligation of biotinylated oligonucleotides to forum domains: Analysis of the data obtained for human rDNA units.
DNA double-strand breaks (DSBs) are associated with different physiological and pathological processes in different organisms. To understand the role of DSBs in multiple cellular mechanisms, a robust method for genome-wide mapping of chromosomal breaks at one-nucleotide resolution is required. Many years ago, we detected large DNA fragments migrating from DNA-agarose plugs in pulsed-field gels, which we named 'forum domains' [1,2]. Recently, we developed a method for genome-wide mapping of DSBs that produces these 50-150 kb DNA domains using microarrays or 454 sequencing (Tchurikov et al., 2011; 2013). Now we have used Illumina sequencing to map DSBs in repetitive rDNA units in human HEK293T cells. Here we describe in detail the experimental design and bioinformatics analysis of the data deposited in the Gene Expression Omnibus with accession number GSE49302 and associated with the study published in the Journal of Molecular Cell Biology (Tchurikov et al., 2014).
Engelhardt Institute of Molecular Biology, Moscow, Russia.
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NA
Tchurikov
Engelhardt Institute of Molecular Biology, Moscow, Russia.
O V
OV
Kretova
Engelhardt Institute of Molecular Biology, Moscow, Russia.
D M
DM
Fedoseeva
Engelhardt Institute of Molecular Biology, Moscow, Russia.
V R
VR
Chechetkin
Engelhardt Institute of Molecular Biology, Moscow, Russia.
M A
MA
Gorbacheva
Engelhardt Institute of Molecular Biology, Moscow, Russia.
A A
AA
Karnaukhov
Engelhardt Institute of Molecular Biology, Moscow, Russia.
G I
GI
Kravatskaya
Engelhardt Institute of Molecular Biology, Moscow, Russia.
Y V
YV
Kravatsky
Bioinformatics; Double-strand breaks; Forum domains; Fragile sites; HEK293T; rDNA