Effects of MK-801 concentration on cell proliferation in rats with focal cerebral ischemia-reperfusion.
We explored the relationship between MK-801 concentration and neural stem cell proliferation in rats with focal cerebral ischemia-reperfusion (FCIR). A total of 60 male Sprague Dawley rats were randomized into control (six rats), sham-operation (six rats), operation (12 rats), and MK-801 groups. The MK-801 group comprised 36 rats that were subjected to different doses of MK-801 (0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mg/kg). Suture occlusion was used to establish an ischemia reperfusion model of middle cerebral artery occlusion (MCAO); 30 min before establishing the FCIR model, the MK-801 group rats were intraperitoneally injected with different doses of MK-801, while the sham-operation and control groups were injected with normal saline. Seven days after model establishment, bromodeoxyuridine-positive cerebral cortex cells adjacent to the focus of infarction were labeled for immunohistochemistry. MK-801 at a concentration of 0.4 mg/kg prevented endogenous neural stem cell proliferation, and this inhibitory effect was strengthened with increasing MK-801 concentration, especially at concentrations greater than 0.8 mg/kg. MK-801 inhibits endogenous neural stem cell proliferation in rats with FCIR, and the inhibitory effect is strengthened with increasing MK-801 concentration.
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
Y K
YK
Gui
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
L L
LL
Su
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
X L
XL
Niu
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
P
P
Zeng
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
R R
RR
Fang
Department of Pharmacy, Xinxiang Medical University, Xinxiang, China.
H X
HX
Lv
Department of Neurology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
P
P
Zhang
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D016291:Dizocilpine Maleate / Q000627:therapeutic use*; D018691:Excitatory Amino Acid Antagonists / Q000627:therapeutic use*; D008297:Male; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*
D018691:Excitatory Amino Acid Antagonists; D016291:Dizocilpine Maleate