A Novel Electrochemiluminescence Immunosensor for the Analysis of HIV-1 p24 Antigen Based on P-RGO@Au@Ru-SiO₂ Composite.
Ru(bpy)3(2+)-doped silica (Ru-SiO2) nanoparticles and gold-nanoparticle-decorated graphene (P-RGO@Au) were combined to form a P-RGO@Au@Ru-SiO2 composite. The composite was used to develop a novel sandwich-type electrochemiluminescence immunosensor for the analysis of HIV-1 p24 antigen. The composite worked as carrier to immobilize target antibody and to build a sandwich-type electrochemiluminescence immunosensor through an interaction between antigen and antibody. Importantly, high ECL signal could be obtained due to the large amounts of Ru(bpy)3(2+) molecules per Ru-SiO2 nanoparticle. The P-RGO@Au composite with good conductivity and high surface area not only accelerated the electron transfer rate but also improved the loading of both ECL molecules and capture antibody, which could further increase the ECL response and result in high sensitivity. Taking advantage of both Ru-SiO2 nanoparticles and the P-RGO@Au composite, the proposed immunosensor exhibited a linear range from 1.0 × 10(-9) to 1.0 × 10(-5) mg mL(-1) with a detection limit of 1.0 × 10(-9) mg mL(-1) for HIV-1 p24 antigen. The proposed ECL immunosensor was used to analyze HIV-1 p24 antigen in human serum, and satisfactory recoveries were obtained, indicating that the proposed method is promising for practical applications in the clinical diagnosis of HIV infection.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, China.|University of the Chinese Academy of Sciences , Beijing 100049, China.
Limin
L
Zhou
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, China.
Jianshe
J
Huang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, China.|National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University , Changchun 130012, China.
Bin
B
Yu
Nanochemistry Research Institute, Department of Chemistry, Curtin University , GPO Box U1987, Perth, Western Australia 6845, Australia.
Yang
Y
Liu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun 130022, China.
Tianyan
T
You
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D016655:HIV Core Protein p24 / Q000737:chemistry*; D015658:HIV Infections / Q000097:blood*; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D012428:Ruthenium / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000906:Antibodies; D016655:HIV Core Protein p24; D006046:Gold; D012822:Silicon Dioxide; D006108:Graphite; D012428:Ruthenium
HIV-1 p24 antigen; Ru(bpy)32+-doped silica nanoparticle; electrochemiluminescence; graphene; immunosensor