Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana.
In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types [1]. Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) [4]. Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
Daniel
D
Slane
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
Jixiang
J
Kong
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
Markus
M
Schmid
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany ; Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.
Gerd
G
Jürgens
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
Martin
M
Bayer
Arabidopsis thaliana; Gene expression; Microarray; Nuclear and cellular transcriptome; Pro-embryo and suspensor transcriptome