Properties and Mechanobiological Behavior of Bovine Nasal Septum Cartilage.
Bovine nasal septum (BNS) is a source of non-load bearing hyaline cartilage. Little information is available on its mechanical and biological properties. The aim of this work was to assess the characteristics of BNS cartilage and investigate its behavior in in vitro mechanobiological experiments. Mechanical tests, biochemical assays, and microscopic assessment were performed for tissue characterization. Compressions tests showed that the tissue is viscoelastic, although values of elastic moduli differ from the ones of other cartilaginous tissues. Water content was 78 ± 1.4%; glycosaminoglycans and collagen contents-measured by spectrophotometric assay and hydroxyproline assay-were 39 ± 5% and 25 ± 2.5% of dry weight, respectively. Goldner's Trichrome staining and transmission electron microscopy proved isotropic cells distribution and results of earlier cell division. Furthermore, gene expression was measured after uniaxial compression, showing variations depending on compression time as well as trends depending on equilibration time. In conclusion, BNS has been characterized at several levels, revealing that bovine nasal tissue is regionally homogeneous. Results suggest that, under certain conditions, BNS could be used to perform in vitro cartilage loading experiments.
Clinic for Masticatory Disorders, Removable Prosthodontics and Special Care, Center for Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland.|Oral Biotechnology & Bioengineering, Department of Cranio-Maxillofacial Surgery, University Hospital Zurich, Zurich, Switzerland.
Maria Rita
MR
Correro-Shahgaldian
Clinic for Masticatory Disorders, Removable Prosthodontics and Special Care, Center for Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland.
Jasmin
J
Introvigne
Oral Biotechnology & Bioengineering, Department of Cranio-Maxillofacial Surgery, University Hospital Zurich, Zurich, Switzerland.
Chafik
C
Ghayor
Oral Biotechnology & Bioengineering, Department of Cranio-Maxillofacial Surgery, University Hospital Zurich, Zurich, Switzerland.
Franz E
FE
Weber
Clinic for Masticatory Disorders, Removable Prosthodontics and Special Care, Center for Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland.
Luigi M
LM
Gallo
Clinic for Masticatory Disorders, Removable Prosthodontics and Special Care, Center for Dental Medicine, University of Zurich, Plattenstrasse 11, 8032, Zurich, Switzerland. Vera.Colombo@zzm.uzh.ch.
Vera
V
Colombo
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002417:Cattle; D019245:Compressive Strength / Q000502:physiology*; D055119:Elastic Modulus; D009300:Nasal Septum / Q000166:cytology / Q000378:metabolism; D016474:Weight-Bearing / Q000502:physiology
Cartilage; Collagen type II; Elastic modulus; Mechanobiology; Proteoglycans