VITRIFICATION OF THAI NATIVE CATTLE OOCYTES: EFFECTS OF ETHYLENE GLYCOL CONCENTRATIONS AND EXPOSURE TIME, LINOLEIC ACID ALBUMIN AND CHOLESTEROL-LOADED METHYL-B-CYCLODEXTRIN.
The present study aimed to improve the oocyte vitrification procedure for preservation of Thai native cattle genetic resources. In Experiment I, oocytes were exposed to various doses (2%, 4% and 6%) of ethylene glycol (EG) in vitrification solution I (VS-I) for different equilibration times (10 or 20 min) before being exposed to VS-II and then subjected to vitrification. Experiment II was divided into two parts: (a) oocytes were matured in medium supplemented with linoleic acid albumin (LAA) (1% or 2%) and then vitrified; (b) matured oocytes were preincubated with cholesterol-loaded methyl-β-cyclodextrin (CLC) (1% or 2%) and then vitrified. Equilibration of oocytes by exposure to 6% EG in VS-I for 10 min (Experiment I), and in vitro maturation of immature oocytes in medium supplementation with 2% LAA (Experiment II) were the most effective methods; vitrified/thawed oocytes showed higher rates of survival and subsequent embryonic development compared with the other experimental groups.
Jakkhaphan
J
Chasombat
Thevin
T
Vongpralub
Saksiri
S
Sirisathien
Yupin
Y
Phasuk
Pronjit
P
Sonseeda
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000008:administration & dosage / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D019855:Ethylene Glycol / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D019787:Linoleic Acid / Q000494:pharmacology*; D008297:Male; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D058989:Vitrification; D047392:beta-Cyclodextrins / Q000737:chemistry
D000418:Albumins; D003451:Cryoprotective Agents; D004337:Drug Carriers; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol; D019787:Linoleic Acid; D019855:Ethylene Glycol