Anti-biofilm activity of zinc oxide and hydroxyapatite nanoparticles as dental implant coating materials.
OBJECTIVE
Dental implants are prone to failure as a result of bacterial biofilm accumulation. Such biofilms are often resistant to traditional antimicrobials and the use of nanoparticles as implant coatings may offer a means to control infection over a prolonged period. The objective of this study was to determine the antibiofilm activity of nanoparticulate coated titanium (Ti) discs using a film fermenter based system.


METHODS
Metal oxide nanoparticles of zinc oxide (nZnO), hydroxyapatite (nHA) and a combination (nZnO+nHA) were coated using electrohydrodynamic deposition onto Ti discs. Using human saliva as an inoculum, biofilms were grown on coated discs for 96 h in a constant depth film fermenter under aerobic conditions with artificial saliva and peri-implant sulcular fluid. Viability assays and biofilm thickness measurements were used to assess antimicrobial activity.


RESULTS
Following 96 h, reduced numbers of facultatively anaerobic and Streptococcus spp. on all three nano-coated surfaces were demonstrated. The proportion of non-viable microorganisms was shown to be higher on nZnO and composite (nZnO+nHA) coated surfaces at 96 h compared with nHA coated and uncoated titanium. Biofilm thickness comparison also demonstrated that nZnO and composite coatings to be the most effective.


CONCLUSIONS
The findings support the use of coating Ti dental implant surfaces with nZnO to provide an antimicrobial function.


CONCLUSIONS
Current forms of treatment for implant associated infection are often inadequate and may result in chronic infection requiring implant removal and resective/regenerative procedures to restore and reshape supporting tissue. The use of metal oxide nanoparticles to coat implants could provide osteoconductive and antimicrobial functionalities to prevent failure.
Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, UK.
Elham H
EH
Abdulkareem
Institute of Dentistry, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, UK; Department of Mechanical Engineering, University College London, UK. Electronic address: Kaveh.memarzadeh@qmul.ac.uk.
K
K
Memarzadeh
Institute of Dentistry, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, UK.
R P
RP
Allaker
Department of Mechanical Engineering, University College London, UK.
J
J
Huang
Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, UK.
J
J
Pratten
Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, UK.
D
D
Spratt
D000180:Acrylic Resins / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D001861:Bone Regeneration / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003188:Composite Resins / Q000737:chemistry; D015921:Dental Implants / Q000382:microbiology*; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology; D019232:Dental Restoration Failure; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D057873:Peri-Implantitis / Q000382:microbiology / Q000517:prevention & control; D011140:Polyurethanes / Q000737:chemistry; D012463:Saliva / Q000382:microbiology; D013291:Streptococcus / Q000187:drug effects / Q000254:growth & development; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry / Q000494:pharmacology*
D000180:Acrylic Resins; D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; C029012:Composite Dental Resin; D003188:Composite Resins; D015921:Dental Implants; D003764:Dental Materials; D011140:Polyurethanes; D017886:Durapatite; D014025:Titanium; D015034:Zinc Oxide
Antimicrobial; Biofilm; Coatings; Nanoparticles; Peri-implantitis; Zinc oxide