Validation of a fast UPLC-MS/MS method for quantitative analysis of opioids, cocaine, amphetamines (and their derivatives) in human whole blood.
BACKGROUND
Conventional methods for analysis of drugs of abuse require multiple assays which can be both expensive and time-consuming. This work describes a novel, rapid, simple and sensitive method for the quantification of 14 illicit drugs and their metabolites in whole blood. Results/methodology: This method employed a rapid liquid-liquid sample extraction of whole blood followed by UPLC-MS/MS analysis. Calibration curves were validated for analysis of appropriate concentrations. Inter- and intra-assay variations were <14.8%. Deviation of accuracy was <14.9% from target concentration for each quality control level.


CONCLUSIONS
This work described the development and the full validation of a precise, sensitive and accurate assay. After validation, this new assay was successfully applied to routine toxicological analysis.
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Catherine
C
Feliu
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Hervé
H
Millart
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Hélène
H
Guillemin
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Damien
D
Vautier
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Laurent
L
Binet
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Aurelie
A
Fouley
Department of Pharmacology, EA3801, SFR CAP-santé, Reims University Hospital, 51, rue Cognacq-Jay, 51095 Reims Cedex, France.
Zoubir
Z
Djerada
D000662:Amphetamines / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D000701:Analgesics, Opioid / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D003042:Cocaine / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D059625:Liquid-Liquid Extraction; D011786:Quality Control; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D054928:Validation Studies as Topic
D000662:Amphetamines; D000701:Analgesics, Opioid; D003042:Cocaine