Identification of microRNA-like RNAs from Curvularia lunata associated with maize leaf spot by bioinformation analysis and deep sequencing.
Deep sequencing of small RNAs is a useful tool to identify novel small RNAs that may be involved in fungal growth and pathogenesis. In this study, we used HiSeq deep sequencing to identify 747,487 unique small RNAs from Curvularia lunata. Among these small RNAs were 1012 microRNA-like RNAs (milRNAs), which are similar to other known microRNAs, and 48 potential novel milRNAs without homologs in other organisms have been identified using the miRBase© database. We used quantitative PCR to analyze the expression of four of these milRNAs from C. lunata at different developmental stages. The analysis revealed several changes associated with germinating conidia and mycelial growth, suggesting that these milRNAs may play a role in pathogen infection and mycelial growth. A total of 8334 target mRNAs for the 1012 milRNAs that were identified, and 256 target mRNAs for the 48 novel milRNAs were predicted by computational analysis. These target mRNAs of milRNAs were also performed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. To our knowledge, this study is the first report of C. lunata's milRNA profiles. This information will provide a better understanding of pathogen development and infection mechanism.
Institute of Applied Microbiology and Plant Pathogenic Fungi, College of Agronony, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, People's Republic of China.|State Key Laboratory of Crop Stress Biology in Arid Regions, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.
Tong
T
Liu
Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, 3190 Maile Way, Honolulu, HI, 96822, USA.
John
J
Hu
Institute of Applied Microbiology and Plant Pathogenic Fungi, College of Agronony, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, People's Republic of China.
Yuhu
Y
Zuo
Institute of Applied Microbiology and Plant Pathogenic Fungi, College of Agronony, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, People's Republic of China.
Yazhong
Y
Jin
Institute of Applied Microbiology and Plant Pathogenic Fungi, College of Agronony, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, People's Republic of China. liutongamy@sina.com.
Jumei
J
Hou
D001203:Ascomycota / Q000235:genetics* / Q000472:pathogenicity; D015966:Gene Expression Regulation, Fungal; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D012331:RNA, Fungal / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000382:microbiology
D035683:MicroRNAs; D012331:RNA, Fungal; D012333:RNA, Messenger
Curvularia lunata; HiSeq deep sequencing; Small RNA; milRNA