17β Estradiol Modulates Perfusion Pressure and Expression of 5-LOX and CYP450 4A in the Isolated Kidney of Metabolic Syndrome Female Rats.
Prevalence of metabolic syndrome and progression of nephropathy depend on sex. We examined a protective effect of estradiol against nephropathy in metabolic syndrome through the modulation of the arachidonic acid metabolism by activating the 5-lipoxygenase and cytochrome p450 4A pathways. 28 female Wistar rats were divided into four groups of seven animals each: control, intact metabolic syndrome, ovariectomized metabolic syndrome, and metabolic syndrome ovariectomized plus estradiol. Blood pressure, body weight, body fat, triglycerides, insulin, HOMA-index, albuminuria, and TNF-α were increased in ovariectomized metabolic syndrome rats (p < 0.001). The perfusion pressure in isolated kidneys of ovariectomized metabolic syndrome rats in presence of 4 μg of arachidonic acid was increased. The inhibitors of the arachidonic acid metabolism Baicalein, Miconazole, and Indomethacin in these rats decreased the perfusion pressure by 57.62%, 99.83%, and 108.5%, respectively and they decreased creatinine clearance and the arachidonic acid percentage. Phospholipase A2 expression in the kidney of ovariectomized metabolic syndrome rats was not modified. 5-lipoxygenase was increased in metabolic syndrome ovariectomized rats while cytochrome p450 4A was decreased. In conclusion, the loss of estradiol increases renal damage while the treatment with estradiol benefits renal function by modulating arachidonic acid metabolism through the 5-lipoxygenase and cytochrome p450 4A pathways.
Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Sección XVI, 14080 Tlalpan, DF, Mexico.
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Zúñiga-Muñoz
Department of Physiology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Sección XVI, 14080 Tlalpan, DF, Mexico.
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Guarner Lans
Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Sección XVI, 14080 Tlalpan, DF, Mexico.
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Soria-Castro
Department of Reproduction Biology, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán", Vasco de Quiroga 15, Sección XVI, 14000 Tlalpan, DF, Mexico.
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Diaz-Diaz
Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Sección XVI, 14080 Tlalpan, DF, Mexico.
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Torrico-Lavayen
Animal Facility Services and Experimental Surgery, Facultad de Medicina Universidad La Salle, Avenue De las Fuentes 17, 14000 Tlalpan, DF, Mexico.
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Tena-Betancourt
Department of Pathology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1, Sección XVI, 14080 Tlalpan, DF, Mexico.
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Pérez-Torres