Fluorinated Sterols Are Suicide Inhibitors of Ergosterol Biosynthesis and Growth in Trypanosoma brucei.
Trypanosoma brucei, the causal agent for sleeping sickness, depends on ergosterol for growth. Here, we describe the effects of a mechanism-based inhibitor, 26-fluorolanosterol (26FL), which converts in vivo to a fluorinated substrate of the sterol C24-methyltransferase essential for sterol methylation and function of ergosterol, and missing from the human host. 26FL showed potent inhibition of ergosterol biosynthesis and growth of procyclic and bloodstream forms while having no effect on cholesterol biosynthesis or growth of human epithelial kidney cells. During exposure of cloned TbSMT to 26-fluorocholesta-5,7,24-trienol, the enzyme is gradually killed as a consequence of the covalent binding of the intermediate C25 cation to the active site (kcat/kinact = 0.26 min(-1)/0.24 min(-1); partition ratio of 1.08), whereas 26FL is non-productively bound. These results demonstrate that poisoning of ergosterol biosynthesis by a 26-fluorinated Δ(24)-sterol is a promising strategy for developing a new treatment for trypanosomiasis.
Center for Chemical Biology and Department of Chemistry & Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA; Institute of Chemistry and Biomedical Sciences, Nanjing University, 22 Hankou Road, Nanjing, Jiangsu, 210093, P.R. China.
David J
DJ
Leaver
Center for Chemical Biology and Department of Chemistry & Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA.
Presheet
P
Patkar
Department of Microbiology and Immunology, Meharry Medical College, 1005 Doctor D. B. Todd Jr. Boulevard, Nashville, TN 37208, USA.
Ujjal K
UK
Singha
Center for Chemical Biology and Department of Chemistry & Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA.
Matthew B
MB
Miller
Center for Chemical Biology and Department of Chemistry & Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA.
Brad A
BA
Haubrich
Department of Microbiology and Immunology, Meharry Medical College, 1005 Doctor D. B. Todd Jr. Boulevard, Nashville, TN 37208, USA.
Minu
M
Chaudhuri
Center for Chemical Biology and Department of Chemistry & Biochemistry, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA. Electronic address: wdavid.nes@ttu.edu.
W David
WD
Nes
D000977:Antiparasitic Agents / Q000737:chemistry / Q000494:pharmacology; D004875:Ergosterol / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008401:Gas Chromatography-Mass Spectrometry; D057809:HEK293 Cells; D054879:Halogenation; D006801:Humans; D015394:Molecular Structure; D013261:Sterols / Q000737:chemistry / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D000977:Antiparasitic Agents; D013261:Sterols; D004875:Ergosterol