Identification of QTLs Conferring Resistance to Deltamethrin in Culex pipiens pallens.
Culex pipiens pallens is the most abundant Culex mosquito species in northern China and is an important vector of bancroftian filariasis and West Nile virus. Deltamethrin is an insecticide that is widely used for mosquito control, however resistance to this and other insecticides has become a major challenge in the control of vector-borne diseases that appear to be inherited quantitatively. Furthermore, the genetic basis of insecticide resistance remains poorly understood. In this study, quantitative trait loci (QTL) mapping of resistance to deltamethrin was conducted in F2 intercross segregation populations using bulked segregation analysis (BSA) and amplified fragment length polymorphism markers (AFLP) in Culex pipiens pallens. A genetic linkage map covering 381 cM was constructed and a total of seven QTL responsible for resistance to deltamethrin were detected by composite interval mapping (CIM), which explained 95% of the phenotypic variance. The major QTL in linkage group 2 accounted for 62% of the variance and is worthy of further study. 12 AFLP markers in the map were cloned and the genomic locations of these marker sequences were determined by applying the Basic Local Alignment Search Tool (BLAST) tool to the genome sequence of the closely related Culex quinquefasciatus. Our results suggest that resistance to deltamethrin is a quantitative trait under the control of a major QTL in Culex pipiens pallens. Cloning of related AFLP markers confirm the potential utility for anchoring the genetic map to the physical map. The results provide insight into the genetic architecture of the trait.
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Feifei
F
Zou
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Chen
C
Chen
Program in Public Health, University of California Irvine, Irvine, California, United States of America.
Daibin
D
Zhong
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Bo
B
Shen
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Donghui
D
Zhang
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Qin
Q
Guo
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Weijie
W
Wang
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Jing
J
Yu
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Yuan
Y
Lv
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Zhentao
Z
Lei
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Kai
K
Ma
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Lei
L
Ma
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu, PR China.
Changliang
C
Zhu
Program in Public Health, University of California Irvine, Irvine, California, United States of America.
Guiyun
G
Yan
D000818:Animals; D003465:Culex / Q000187:drug effects / Q000235:genetics*; D008040:Genetic Linkage; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology*; D009032:Mosquito Control; D009570:Nitriles / Q000494:pharmacology*; D010641:Phenotype; D011722:Pyrethrins / Q000494:pharmacology*; D040641:Quantitative Trait Loci
D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin