Lipidomics analysis of follicular fluid by ESI-MS reveals potential biomarkers for ovarian endometriosis.
OBJECTIVE
The aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF).


METHODS
The control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry.


RESULTS
We observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance.


CONCLUSIONS
This analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Fernanda Bertuccez
FB
Cordeiro
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Thais Regiani
TR
Cataldi
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Kayla Jane
KJ
Perkel
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Lívia
L
do Vale Teixeira da Costa
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Raquel Cellin
RC
Rochetti
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Juliana
J
Stevanato
Institute of Chemistry, University of Campinas, Campinas, SP, Brazil.
Marcos Nogueira
MN
Eberlin
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil. danielsuslik@gmail.com.
Daniel Suslik
DS
Zylbersztejn
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Agnaldo Pereira
AP
Cedenho
Human Reproduction Section, Division of Urology, Department of Surgery, Sao Paulo Federal University, R Embau, 231, São Paulo, SP, 04039-060, Brazil.
Edson Guimarães Lo
EG
Turco
D015415:Biomarkers / Q000378:metabolism*; D004715:Endometriosis / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D055432:Metabolomics; D021241:Spectrometry, Mass, Electrospray Ionization
D015415:Biomarkers; D008055:Lipids
Endometrioma; Endometriosis; Follicular fluid; IVF; Mass spectrometry, lipids; Metabolomics