Repeated Superovulation via PMSG/hCG Administration Induces 2-Cys Peroxiredoxins Expression and Overoxidation in the Reproductive Tracts of Female Mice.
Superovulation induced by exogenous gonadotropin treatment (PMSG/hCG) increases the number of available oocytes in humans and animals. However, Superovulatory PMSG/hCG treatment is known to affect maternal environment, and these effects may result from PMSG/hCG treatment-induced oxidative stress. 2-Cys peroxiredoxins (2-Cys Prxs) act as antioxidant enzymes that protect cells from oxidative stress induced by various exogenous stimuli. Therefore, the objective of this study was to test the hypothesis that repeated PMSG/hCG treatment induces 2-Cys Prx expression and overoxidation in the reproductive tracts of female mice. Immunohistochemistry and western blotting analyses further demonstrated that, after PMSG/hCG treatment, the protein expression levels of 2-Cys Prxs increased most significantly in the ovaries, while that of Prx1 was most affected by PMSG/hCG stimulation in all tissues of the female reproductive tract. Repeated PMSG/hCG treatment eventually leads to 2-Cys Prxs overoxidation in all reproductive organs of female mice, and the abundance of the 2-Cys Prxs-SO2/3 proteins reported here supports the hypothesis that repeated superovulation induces strong oxidative stress and damage to the female reproductive tract. Our data suggest that excessive oxidative stress caused by repeated PMSG/hCG stimulation increases 2-Cys Prxs expression and overoxidation in the female reproductive organs. Intracellular 2-Cys Prx therefore plays an important role in maintaining the reproductive organ environment of female mice upon exogenous gonadotropin treatment.
School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Korea.
Sun-Ji
SJ
Park
Embryology Laboratory, Neway Fertility, 115 East 57th Street Suite 500 New York, NY 10022, USA.
Tae-Shin
TS
Kim
Cancer Research Institute and Department of Pathology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea.
Jin-Man
JM
Kim
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Korea.
Kyu-Tae
KT
Chang
School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Korea.
Hyun-Shik
HS
Lee
School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 702-701, Korea.
Dong-Seok
DS
Lee
D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage* / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000008:administration & dosage* / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000378:metabolism; D051379:Mice; D009928:Organ Specificity; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D054464:Peroxiredoxins / Q000378:metabolism*; D013480:Superovulation / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D006063:Chorionic Gonadotropin; D004338:Drug Combinations; D006064:Gonadotropins, Equine; D018398:Homeodomain Proteins; C036835:PMSG-HCG; C489936:Prrx1 protein, mouse; D054464:Peroxiredoxins
2-Cys peroxiredoxins; exogenous gonadotropin; female mice; oxidative stress; reproductive organ environment