Effects of lipopolysaccharide on changes in red blood cells in a mice endotoxemia model.
The aim of this study was to evaluate changes in RBC aggregation and deformability over 24 hr and suggest specific shear stress values for detecting RBC deformability in a mouse endotoxemia model using lipopolysaccharide (LPS).Six-week-old male BALB/c mice received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and T1/2 were measured to assess RBC aggregation, and elongation indices (EIs) were used to assess RBC deformability at shear stress values of 0.3, 0.5, 1, 3, 7, 10, 15 and 20 Pascals (Pa) 0, 30 min, 1, 2, 4, 6, 9, 12, 18 and 24 hours after the LPS injection.No significant differences were detected in the AIs during the study period, however, T1/2 shortened significantly 2, 6, 12, 18, and 24 hr after the LPS injection. The EIs increased significantly 24 hr after LPS injection at 0.5 and 1 Pa shear stress, whereas it decreased significantly at 10 Pa of shear stress 24 hr after the LPS injection.Altered RBC deformability was detectable 24 hr after the LPS injection and T1/2 may be a sensitive marker for detecting changes in RBC aggregation. The EIs should be measured at 1 Pa to detect changes in RBC deformability in LPS-induced septic mice.
Department of Medicine, School of Medicine, Korea University, Seoul, Korea.
Jeihak
J
Myung
Department of Anaesthesiology and Pain Medicine, Korea University, Korea.
Seol Ju
SJ
Park
Korea Artificial Organ Center, Korea University, Seoul, Korea.
Jaekwan
J
Lim
Department of Anaesthesiology and Pain Medicine, Korea University, Korea.
Yun Hee
YH
Kim
School of Mechanical Engineering, Korea University, Seoul, Korea.
Sehyun
S
Shin
Department of Anaesthesiology and Pain Medicine, Korea University, Korea.
Choon Hak
CH
Lim
D000818:Animals; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability / Q000187:drug effects*; D004912:Erythrocytes / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013314:Stress, Mechanical
D008070:Lipopolysaccharides
Lipopolysaccharide; RBC aggregation; RBC deformability; shear 
stress