Preparation of ⁶⁸Ga-labelled DOTA-peptides using a manual labelling approach for small-animal PET imaging.
(68)Ga-DOTA-peptides are a promising PET radiotracers used in the detection of different tumours types due to their ability for binding specifically receptors overexpressed in these. Furthermore, (68)Ga can be produced by a (68)Ge/(68)Ga generator on site which is a very good alternative to cyclotron-based PET isotopes. Here, we describe a manual labelling approach for the synthesis of (68)Ga-labelled DOTA-peptides based on concentration and purification of the commercial (68)Ga/(68)Ga generator eluate using an anion exchange-cartridge. (68)Ga-DOTA-TATE was used to image a pheochromocytoma xenograft mouse model by a microPET/CT scanner. The method described provides satisfactory results, allowing the subsequent (68)Ga use to label DOTA-peptides. The simplicity of the method along with its implementation reduced cost, makes it useful in preclinical PET studies.
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain. Electronic address: eduardo.romero@ciemat.es.
Eduardo
E
Romero
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
Alfonso
A
Martínez
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
Marta
M
Oteo
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
Angel
A
García
CIEMAT - Research Centre for Energy, Environment and Technology, Biomedical Applications and Pharmacokinetics Unit, Avenida Complutense 40, 28040 Madrid, Spain.
Miguel Angel
MA
Morcillo
D000310:Adrenal Gland Neoplasms / Q000000981:diagnostic imaging; D000818:Animals; D004867:Equipment Design; D005710:Gallium Radioisotopes / Q000302:isolation & purification*; D006573:Heterocyclic Compounds, 1-Ring / Q000302:isolation & purification; D007553:Isotope Labeling / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D064847:Multimodal Imaging; D009842:Oligopeptides / Q000302:isolation & purification; D009942:Organometallic Compounds / Q000302:isolation & purification; D010673:Pheochromocytoma / Q000000981:diagnostic imaging; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000302:isolation & purification*; D014057:Tomography, X-Ray Computed
D005710:Gallium Radioisotopes; D006573:Heterocyclic Compounds, 1-Ring; D009842:Oligopeptides; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; C513399:gallium Ga 68 dotatate
Concentration; DOTA-peptides; Gallium-68; NaOH; Pheochromocytoma; Purification; Radiolabelling