Anti-Neoplastic Cytotoxicity of SN-38-Loaded PCL/Gelatin Electrospun Composite Nanofiber Scaffolds against Human Glioblastoma Cells In Vitro.
Electrospun poly(ε-caprolactone) (PCL)/gelatin (GT) scaffolds were developed to provide controlled release of 7-ethyl-10-hydroxy camptothecin (SN-38). Acetic acid was introduced to improve the miscibility of PCL and GT to produce a homogeneous nanofiber membrane mixture. The effect of SN-38 content in binary mixtures on processability, fiber morphology, water sorption, swelling, and drug release was investigated. Electrospun PCL/GT blend nonwoven fibers showed fiber surface roughness, decreased PCL crystallinity, and increased swelling with increasing drug content of 1, 2, and 4 wt %. Additionally, increasing the SN-38 concentration reduced the degradation rate of the GT. Furthermore, we hypothesize the existence of a drug content saturation point in the monoaxial fiber to explain the different drug release patterns of PG2 compared with those of PG1 and PG4. The matrix also showed good biodegradation and anti-tumor function. Our results demonstrate that SN-38-loaded PCL/GT fibers can be obtained by electrospinning. The SN-38-loaded fibers merit further evaluation as a means to potentially prevent locoregional recurrence following surgical tumor resection.
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Xiaodong
X
Zhu
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Shilei
S
Ni
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Tongliang
T
Xia
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Qingyu
Q
Yao
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Haoyuan
H
Li
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Benlin
B
Wang
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Jiangang
J
Wang
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China.
Xingang
X
Li
Department of Neurosurgery, Qilu Hospital, Shandong University, Jinan 250012, People's Republic of China. Electronic address: suwandong@sina.com.
Wandong
W
Su
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D065546:Drug Liberation; D005780:Gelatin / Q000737:chemistry*; D005909:Glioblastoma / Q000188:drug therapy*; D006801:Humans; D000077146:Irinotecan; D008566:Membranes / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D011091:Polyesters; C016240:polycaprolactone; D000077146:Irinotecan; D005780:Gelatin; D002166:Camptothecin
biomaterial; blood brain barrier; in vitro models; nanotechnology; polymeric drug delivery systems