Genetic polymorphisms of IL28b gene as predictors of response to dual therapy in genotypes 1 and 4-HCV and HIV/HCV-infected patients.
We describe the genotypes and allele distribution of interleukin 28B (IL28B) rs12979860 and rs8099917 single nucleotide polymorphisms (SNPs) in hepatitis C virus (HCV) G1-4 infected patients, to assess predictive ability and to determine whether the combined determination of two IL28B SNPs might improve sustained virologic response (SVR) prediction of both in HCV mono- and HIV/HCV co-infected patients. IL28B SNPs were genotyped in 269 patients, 181 mono- and 88 co-infected, treated with pegylated interferon and ribavirin. Data stratified by HCV mono- and HCV/HIV co-infected patients showed that 58% and 31% of the rs12979860CC carriers and 49% and 21% of the rs8099917TT carriers had SVR. IL28B SNPs, HCV mono-infection and HCV RNA load were associated with SVR as independent predictors in the two study groups as a whole. ROC curve analyses in the two populations separately, based on gender, age, baseline HCV RNA load and rs12979860/rs8099917 revealed similar receiver operating characteristics (ROC) areas under the curve values. Combining the determination of IL28B SNPs, rs8099917 genotyping improved the response prediction in rs12979860CT carriers only in mono-infected patients. In the era of direct-acting antiviral agents, adopting SVR baseline predictors to orientate naïve-patient management represents an important issue. A model involving IL28B SNPs appears able to predict SVR in both populations.
Department of Health Sciences, University of Genoa, Genoa, Italy.|Hygiene Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
Laura
L
Sticchi
Infectious Diseases Unit, IRCCS AOU San Martino-IST, University of Genoa, Genoa, Italy.
Antonio
A
Di Biagio
Department of Health Sciences, University of Genoa, Genoa, Italy.
Marina
M
Sartini
Department of Health Sciences, University of Genoa, Genoa, Italy.
Emanuela
E
Rappazzo
Infectious Diseases Unit, IRCCS AOU San Martino-IST, University of Genoa, Genoa, Italy.
Laura A
LA
Nicolini
Infectious Diseases Unit, Galliera Hospital, Genoa, Italy.
Giovanni
G
Cenderello
Molecular Biology Unit, St. Corona Hospital, Pietra Ligure, Savona, Italy.
Caterina
C
Valle
Gastroenterology and Digestive Endoscopy Unit, St. Corona Hospital, Pietra Ligure, Savona, Italy.
Emilio
E
Azzola
Gastroenterology Unit, San Paolo Hospital , Savona, Italy.
Alessandro
A
Grasso
Infectious Disease Unit, San Paolo Hospital , Savona, Italy.
Pasqualina
P
De Leo
Infectious Disease Unit, Sanremo Hospital, Imperia, Italy.
Alessandra
A
Boldrini
Internal Medicine and Clinical Immunology Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
Maurizio
M
Setti
Infectious Diseases Unit, IRCCS AOU San Martino-IST, University of Genoa, Genoa, Italy.
Roberta
R
Prinapori
Department of Mental Health and Addiction -Local Health Unit 4, Chiavari, Genoa, Italy.
Carolina
C
Lorusso
Hygiene Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
Bianca
B
Bruzzone
Department of Health Sciences, University of Genoa, Genoa, Italy.|Hygiene Unit, IRCCS AOU San Martino-IST, Genoa, Italy.
Giancarlo
G
Icardi
D000328:Adult; D000998:Antiviral Agents / Q000627:therapeutic use*; D060085:Coinfection / Q000188:drug therapy / Q000235:genetics / Q000821:virology; D004359:Drug Therapy, Combination; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy* / Q000235:genetics / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006526:Hepatitis C / Q000188:drug therapy* / Q000235:genetics / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012254:Ribavirin / Q000627:therapeutic use; D016896:Treatment Outcome; D019562:Viral Load; D055815:Young Adult; D000095022:Interferon Lambda
D000998:Antiviral Agents; D016898:Interferon-alpha; D007372:Interferons; D007378:Interleukins; D012254:Ribavirin; C000624353:interferon-lambda, human; D000095022:Interferon Lambda
Hepatitis C virus-G1; Hepatitis C virus-G4; Human immunodeficiency virus; Interleukin-28B rs12979860; Interleukin-28B rs8099917