Watson-Crick hydrogen bonding of unlocked nucleic acids.
We herein describe the synthesis of two new unlocked nucleic acid building blocks containing hypoxanthine and 2,6-diaminopurine as nucleobase moieties and their incorporation into oligonucleotides. The modified oligonucleotides were used to examine the thermodynamic properties of UNA against unmodified oligonucleotides and the resulting thermodynamic data support that the hydrogen bonding face of UNA is Watson-Crick like.
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark.
Niels
N
Langkjær
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, Odense 5230, Denmark. Electronic address: jwe@ifk.sdu.dk.
Jesper
J
Wengel
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland. Electronic address: apa@ibch.poznan.pl.
Anna
A
Pasternak
D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D020029:Base Pairing; D001483:Base Sequence; D006860:Hydrogen Bonding; D007288:Inosine / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D020871:RNA Stability; D013816:Thermodynamics
D009843:Oligoribonucleotides; C033854:2-aminoadenosine; D007288:Inosine; D012313:RNA; D000241:Adenosine
Hydrogen bonding; RNA duplex; Thermodynamics; Unlocked nucleic acids