Evaluation and comparison of the in vitro characteristics and chondrogenic capacity of four adult stem/progenitor cells for cartilage cell-based repair.
Cell-based therapy is being considered as a promising approach to regenerate damaged cartilage. Though, autologous chondrocyte implantation is the most effective strategy currently in use, but is hampered by some drawbacks seeking comprehensive research to surmount existing limitations or introducing alternative cell sources. In this study, we aimed to evaluate and compare the in vitro characteristics and chondrogenic capacity of some easily available adult cell sources for use in cartilage repair which includes: bone marrow-derived mesenchymal stem cells (MSC), adipose tissue-derived MSC, articular chondrocyte progenitors, and nasal septum-derived progenitors. Human stem/progenitor cells were isolated and expanded. Cell's immunophenotype, biosafety, and cell cycle status were evaluated. Also, cells were seeded onto aligned electrospun poly (l-lactic acid)/poly (ε-caprolactone) nanofibrous scaffolds and their proliferation rate as well as chondrogenic potential were assessed. Cells were almost phenotypically alike as they showed similar cell surface marker expression pattern. The aligned nanofibrous hybrid scaffolds could support the proliferation and chondrogenic differentiation of all cell types. However, nasal cartilage progenitors showed a higher proliferation potential and a higher chondrogenic capacity. Though, mostly similar in the majority of the studied features, nasal septum progenitors demonstrated a higher chondrogenic potential that in combination with their higher proliferation rate and easier access to the source tissue, introduces it as a promising cell source for cartilage tissue engineering and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 600-610, 2016.
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Stem Cell Biology and Tissue Engineering Department, Stem Cell Technology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.|Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia.
Abbas
A
Shafiee
Stem Cell Biology and Tissue Engineering Department, Stem Cell Technology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.|Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Mahboubeh
M
Kabiri
Stem Cell Biology and Tissue Engineering Department, Stem Cell Technology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.|Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, M5S 3G5, Canada.
Lida
L
Langroudi
Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
Masoud
M
Soleimani
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Jafar
J
Ai
D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002358:Cartilage, Articular / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007621:Karyotyping; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D009666:Nose / Q000166:cytology; D010641:Phenotype; D011091:Polyesters / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
D015415:Biomarkers; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide)
cartilage tissue engineering; cell therapy; chondrocyte; human mesenchymal stem cells; nanofiber