A Novel Osteoblast/Osteocyte Selection Method in Primary Isolated Chick Bone Cells by Atomic Force Microscopy.
Selection of bone cells, particularly osteoblasts and osteocytes, for analysis of cellular processes and differentiation is a very important issue because bone remodeling is a highly complex and harmonized process, which includes molecular and cellular interactions and communications. In this study, we introduce a novel osteoblast and osteocyte selection method that uses atomic force microscopy and OB7.3, an antibody of Phex, which is a specific protein marker expressed on the surface of osteocytes. The elasticity and Phex expression levels were simultaneously detected by force spectroscopy using the OB7.3-modified atomic force microscopy probe on the bone cell surface. The elastic modulus was different between osteoblasts and osteocytes. Phex expression level was analyzed by the distribution of Phex-OB7.3 rupturing.
Sung-Woong
SW
Han
Takato
T
Tamaki
Taiji
T
Adachi
D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002478:Cells, Cultured; D002645:Chickens; D055119:Elastic Modulus; D018625:Microscopy, Atomic Force / Q000379:methods*; D010006:Osteoblasts / Q000737:chemistry* / Q000166:cytology*; D010011:Osteocytes / Q000737:chemistry* / Q000166:cytology*; D053402:PHEX Phosphate Regulating Neutral Endopeptidase / Q000032:analysis
D053402:PHEX Phosphate Regulating Neutral Endopeptidase