Detection of Mycobacterium tuberculosis with nested polymerase chain reaction analysis in enucleated eye ball in Eales' disease.
Nested polymerase chain reaction (nPCR) was performed on enucleated eyeball for detection of Mycobacterium tuberculosis (M. tb) genome in a patient with Eales' disease. PCR analysis in all previous studies has been done mainly using aqueous, vitreous and epiretinal membranes from these patients. Paraffin wax embedded tissue section of the enucleated eyeball was analyzed by histopathology and nPCR targeting MPB64 gene and IS6110 region of M. tb genome. Lymphocytic infiltration was seen in the vitreous, iris and the retinal tissue. Ziehl Neelsen stain was negative for acid fast bacilli. Caseation necrosis was not seen in any section. Agarose gel electrophoretogram showed positive results with 200 bp specific amplified product targeting MPB64 gene, whereas nPCR targeting IS6110 region was negative. Since biopsy proven M. tb is extremely difficult in ocular tissues due to extensive necrosis, the nPCR technique aided in the diagnosis.
Sri Bhagwan Mahavir Vitreoretinal Services, Medical Research Foundation, Sankara Nethralaya, College Road 18, Nungambakkam, Chennai, 600006, India. dradi27@gmail.com.
Aditya
A
Verma
Department of Uvea and Ocular Pathology, Medical Research Foundation, Chennai, India.
Jyotirmay
J
Biswas
L&T Microbiology Research Centre, Vision Research Foundation, Chennai, India.
L
L
Dhanurekha
L&T Microbiology Research Centre, Vision Research Foundation, Chennai, India.
R
R
Gayathri
L&T Microbiology Research Centre, Vision Research Foundation, Chennai, India.
K
K
Lily Therese
D004269:DNA, Bacterial / Q000032:analysis; D015353:Eye Enucleation; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000150:complications*; D016133:Polymerase Chain Reaction / Q000379:methods*; D031300:Retinal Vasculitis / Q000150:complications*; D014392:Tuberculosis, Ocular / Q000175:diagnosis / Q000382:microbiology*; D055815:Young Adult
D004269:DNA, Bacterial
Eales’ disease; Hypersensitivity reaction; Mycobacterium tuberculosis; Polymerase chain reaction; Tuberculoprotein