Intracellular Ca2+ and related proteins in patients with oral lichen planus.
Oral lichen planus (OLP) is suggested to be a T cell-mediated chronic inflammatory oral mucosal disease. Gene expressed in the oligodendrocyte lineage-myelin basic proteins (Golli-MBP) and stromal interaction molecule 1 (STIM1) are important in the activation and function of T lymphocytes. This study aimed to analyze and compare the expression of Golli-MBP and STIM1 between OLP patients and healthy controls and to analyze the level of intracellular Ca(2+), which is involved in lymphocyte activation. The Ca(2+) fluorescent probe, Fluo-3/AM, was used to test the level of intracellular Ca(2+) in patients with OLP and healthy controls peripheral blood lymphocytes. Golli-MBP and STIM1 mRNA and protein levels were analyzed using quantitative real-time PCR and Western blot, respectively. Following lymphocyte activation, the intracellular Ca(2+) in OLP patients was markedly lower than that in the control group (P < 0.001). In OLP patients, the expression of Golli-MBP mRNA and protein was significantly upregulated compared to those of the control group (P < 0.001). Similarly, OLP patients showed markedly upregulated levels of STIM1 mRNA expression (P < 0.01) and protein compared to healthy controls. The intracellular Ca(2+) of OLP patients was markedly lower than that of healthy controls. This evidence may indicate that Ca(2+) signaling pathways in OLP patients are abnormal. The overexpression of Golli-MBP and STIM1 may play a role in the pathogenesis of OLP.
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China.|Department of Stomatology, Kunshan Hospital of Traditional Chinese Medicine, 189#, Chaoyang Road, Kunshan, 215300, Jiangsu, People's Republic of China.
Jiang-Min
JM
Ma
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China.|Department of Stomatology, Nanjing Red Cross Hospital, 242#, Baixia Road, Nanjing, 210000, Jiangsu, People's Republic of China.
Ran
R
Wang
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China.|Department of Oral Medicine, Affiliated Hospital of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China.
Juan-Yong
JY
Xu
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China. fanyuan65@hotmail.com.|Department of Oral Medicine, Affiliated Hospital of Stomatology, Nanjing Medical University, 136#, Hanzhong Road, Nanjing, 210029, Jiangsu, People's Republic of China. fanyuan65@hotmail.com.
Yuan
Y
Fan
D000328:Adult; D000368:Aged; D002118:Calcium / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D017676:Lichen Planus, Oral / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D000071737:Stromal Interaction Molecule 1 / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
D004676:Myelin Basic Protein; D000071737:Stromal Interaction Molecule 1; D002118:Calcium
Genes of the oligodendrocyte lineage; Intracelluar calcium; Oral lichen planus; Stromal interaction molecule 1