The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study.
The aim of this study was to observe the effect of hypoxia-inducible factor (HIF)-1α on bone regeneration during distraction osteogenesis (DO). Fifty-one New Zealand white rabbits underwent mandibular lengthening with a distraction rate of 2mm/day, and were divided randomly into three groups (17 in each). Group C rabbits received 20 μg rHIF-1α, group B received 10 μg rHIF-1α, and group A received 100 μl saline injection in the distraction gap every day for 10 days. Radionuclide bone imaging (RBI), computed tomography, dual energy X-ray absorptiometry, radiography, histology, and three-point bend testing were performed. RBI showed that the uptake ratio in group B (1.41 ± 0.25, P=0.013) and group C (1.64 ± 0.37, P<0.001) was higher than that in group A (1.01 ± 0.26). The bone mineralization density and bone mineralization content in group C were highest among the three groups. Radiology and histology findings indicated more callus regeneration in groups C and B. Mechanical testing demonstrated that the ultimate force in group C (289.71 ± 43.31N, n=6) was 1.49-fold (P<0.001) that of group A and 1.20-fold (P=0.012) that of group B. HIF-1α may represent a new agent to promote DO by accelerating osteogenesis and mineralization.
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China. Electronic address: jxw0927@163.com.
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Jiang
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China.
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Zhang
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China.
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Fan
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China.
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Deng
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China.
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Zhu
Department of Stomatology, the First People's Hospital of Chenzhou, Southern Medical University and Institute of Translation Medicine, University of China South, Chenzhou, Hunan Province, China.
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Li
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000494:pharmacology*; D008297:Male; D008334:Mandible / Q000000981:diagnostic imaging* / Q000601:surgery*; D019857:Osteogenesis, Distraction / Q000379:methods*; D011817:Rabbits; D011859:Radiography
D051795:Hypoxia-Inducible Factor 1, alpha Subunit
distraction osteogenesis; hypoxia-inducible factor; mandible; rabbit