Bioassay of cyanoacrylate tissue adhesives used for intraperitoneal mesh fixation.
This study examines the intraperitoneal behavior of two cyanoacrylate tissue adhesives: Ifabond®and a new, non-marketed octyl cyanoacrylate adhesive (OCA) used for the intraperitoneal fixation of a laminar expanded polytetrafluoroethylene (ePTFE) mesh.



In 36 New Zealand White rabbits, 3 × 3 cm (n = 24) or 1.5 × 3 cm (n = 12) fragments of ePTFE mesh (Preclude®, Gore, Flagstaff, USA) were fixed to the parietal peritoneum using OCA or Ifabond®. Peritoneal fluid was obtained at the time of implant and at 2 weeks postimplant for determination of the cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α). At 14 or 90 days postsurgery, the animals were euthanized and the meshes excised to assess host tissue incorporation, the macrophage response, apoptosis and fixation strength (T-peel tensiometry).



Peritoneal fluid IL-6 and TNF-α concentrations were similar in the OCA and Ifabond®groups. Both adhesives gave rise to adequate mesothelialization of the laminar ePTFE. Macrophage counts were similar for the two study groups, but a significantly increase in macrophage response was observed from 14 to 90 days for Ifabond®. At 90 days postimplant, apoptotic cell counts was lower for the implants fixed with OCA and a fixation strength was significantly lower for OCA.



Despite similar cytokine levels at 2 weeks and similar host tissue incorporation observed for the meshes fixed with the two adhesives, the use of Ifabond®gave rise to a greater apoptosis rate, although this adhesive provided a stronger fixation bond. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 312-319, 2017.
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Juan M
JM
Bellón
Polymeric Biomaterials Group, Polymer Science and Technology Institute-Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Mar
M
Fernández-Gutiérrez
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Marta
M
Rodríguez
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Sandra
S
Sotomayor
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Bárbara
B
Pérez-Köhler
Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Andree
A
Kuhnhardt
Department of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcalá. Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN). Alcalá de Henares, Madrid, Spain.
Gemma
G
Pascual
Polymeric Biomaterials Group, Polymer Science and Technology Institute-Consejo Superior de Investigaciones Científicas (ICTP-CSIC), Networking Research Centre on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Julio
J
San Román
D000818:Animals; D003487:Cyanoacrylates / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D005465:Fluorocarbon Polymers / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008422:Materials Testing; D011817:Rabbits; D013526:Surgical Mesh; D014014:Tissue Adhesives / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D003487:Cyanoacrylates; D005465:Fluorocarbon Polymers; D015850:Interleukin-6; D014014:Tissue Adhesives; D014409:Tumor Necrosis Factor-alpha; C010946:polytetrafluoroethane
cyanoacrylates; laminar ePTFE prosthesis; laparoscopic hernia repair; mesh fixation