A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells.
Due to the lack of a proper imaging approach, a veracious pH map of normal and abnormal cell is still rare. In this work, we presented a rhodamine-salicylaldehyde combination (Rh-SA2) as a novel pH probe, which has dual sensitive units for both acidic and basic environment. This dual sensitive probe acts like a chameleon in living cells and offers the doubling guarantees for endocellular pH mapping. Moreover, a quantitative measurement of cellular pH changes was allowed and the endocellular pH values under drug-associated stimuli were also investigated.
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Kang-Kang
KK
Yu
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Ji-Ting
JT
Hou
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.|State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, Sichuan University, No. 17, People's South Road, Chengdu, 610041, P.R. China.
Kun
K
Li
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Qian
Q
Yao
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Jin
J
Yang
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Ming-Yu
MY
Wu
State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, Sichuan University, No. 17, People's South Road, Chengdu, 610041, P.R. China.
Yong-Mei
YM
Xie
Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, 610064, P.R. China.
Xiao-Qi
XQ
Yu
D000447:Aldehydes / Q000737:chemistry*; D058948:Cell Tracking / Q000379:methods*; D003952:Diagnostic Imaging; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012235:Rhodamines / Q000737:chemistry*
D000447:Aldehydes; D005456:Fluorescent Dyes; D012235:Rhodamines; C013243:salicylaldehyde