Helix aspersa gelatin as an emulsifier and emulsion stabilizer: functional properties and effects on pancreatic lipolysis.
Emulsions are widely used in food and pharmaceutical applications for the encapsulation, solubilization, entrapment, and controlled delivery of active ingredients. In order to fulfill the increasing demand for clean label excipients, natural polymers could be used to replace the potentially irritative synthetic surfactants used in emulsion formulation. In the present study, we have studied the properties of oil-in-water emulsions prepared with land snail gelatin (LSG) as the sole emulsifying agent, extracted and described for the first time. LSG was evaluated in terms of proximate composition, oil and water holding capacity, emulsifying and foaming properties, color and amino acid composition. Emulsions of trioctanoylglycerol (TC8) and olive oil were made at different gelatin/oil ratios and changes in droplet-size distribution were determined. The superior emulsifying properties of LSG, the susceptibility of gelatin protein emulsions increasing flocculation on storage, and the coalescence of gelatin emulsions following centrifugation were demonstrated. Furthermore, the effect of LSG on the activity of turkey pancreatic lipase (TPL) was evaluated through the pH-stat methodology with TC8 and olive oil emulsions. The LSG affected the TPL activity in a concentration-dependent way. Our results showed that LSG, comparably to gum arabic, increases the pancreatic lipase activity and improves its stability at the oil-water interface.
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, PB 1173, Km 4 Road Soukra, 3038 Sfax, Tunisia. zaraizied@hotmail.fr.
Zied
Z
Zarai
Enzymes & Bioconversion Unit, National School of Engineers of Sfax, University of Sfax, PB 1173, Km 4 Road Soukra, 3038 Sfax, Tunisia.
Rafik
R
Balti
Enzymes & Bioconversion Unit, National School of Engineers of Sfax, University of Sfax, PB 1173, Km 4 Road Soukra, 3038 Sfax, Tunisia.
Assaâd
A
Sila
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, PB 1173, Km 4 Road Soukra, 3038 Sfax, Tunisia. zaraizied@hotmail.fr.
Yassine
Y
Ben Ali
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, PB 1173, Km 4 Road Soukra, 3038 Sfax, Tunisia. zaraizied@hotmail.fr.
Youssef
Y
Gargouri
D000818:Animals; D045703:Emulsifying Agents / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006372:Helix, Snails / Q000737:chemistry*; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D000069463:Olive Oil / Q000737:chemistry / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology; D017550:Spectroscopy, Fourier Transform Infrared; D014422:Turkeys / Q000378:metabolism
D045703:Emulsifying Agents; D004655:Emulsions; D000069463:Olive Oil; D005780:Gelatin; D008049:Lipase