UVB Dependence of Quantum Dot Reactive Oxygen Species Generation in Common Skin Cell Models.
Studies have shown that UVB can slightly increase the penetration of nanoparticles through skin and significantly alter skin cell biology, thus it is important to understand if and how UVB may impact subsequent nanoparticle skin cell interactions. The research presented herein evaluates the effect of UVB on quantum dot (QD) uptake and reactive oxygen species (ROS) generation in primary keratinocytes, primary melanocytes, and related cell lines. QD exposure induced cell type dependent ROS responses increased by pre-exposing cells to UVB and correlated with the level of QD uptake. Our results suggest that keratinocytes may be at greater risk for QD induced ROS generation than melanocytes, and raise awareness about the differential cellular effects that topically applied nanomaterials may have on UVB exposed skin.
Luke J
LJ
Mortensen
Renea
R
Faulknor
Supriya
S
Ravichandran
Hong
H
Zheng
Lisa A
LA
DeLouise
D002460:Cell Line; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects*; D008422:Materials Testing; D045663:Quantum Dots; D011829:Radiation Dosage; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012867:Skin / Q000378:metabolism* / Q000528:radiation effects; D012869:Skin Absorption / Q000502:physiology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D017382:Reactive Oxygen Species