In Vitro Metabolomic Approach to Hippocampal Neurodegeneration Induced by Trimethyltin.
Search for indicators of neurodegenerative disorders is a hot topic where much research remains to be done. Our aim was to determine proton nuclear magnetic resonance ((1)H-NMR) spectra of brain metabolites in the trimethyltin (TMT) model of neurodegeneration. Male Wistar rats were subjected to TMT or saline and were sacrificed on day 3 or 24 after administration. (1)H-NMR spectrum was measured on the 600 MHz Varian VNMRS spectrometer in nano-probe in the volume of 40 μl of hippocampal extracts. TMT administration resulted in reduction of the hippocampal weight on day 24. Of the sixteen identified metabolite spectra, decreased aspartate and increased glutamine contents were observed in the initial asymptomatic stage of neurodegeneration on day 3 in hippocampal extracts of TMT exposed rats compared to sham animals. Increased myo-inositol content was observed on day 24. The presented data provide further knowledge about this experimental model and putative indicators of neuronal damage.
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia. zdenka.gasparova@savba.sk.
Zdenka
Z
Gasparova
Department of NMR and Mass Spectroscopy, Faculty of Food and Chemical Technology, Slovak University of Technology, Bratislava, Slovakia.
Nada
N
Pronayova
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia.|Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
Veronika
V
Stara
Department of NMR and Mass Spectroscopy, Faculty of Food and Chemical Technology, Slovak University of Technology, Bratislava, Slovakia.
Tibor
T
Liptaj
D000818:Animals; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D055442:Metabolome; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009929:Organ Size; D017208:Rats, Wistar; D014298:Trimethyltin Compounds
D014298:Trimethyltin Compounds; C046488:trimethyltin
Alzheimer disease; Hippocampus; In vitro 1H-NMR spectroscopy; Perchloric acid extracts; Rat; Trimethyltin model of neurodegeneration