Inoculation of paperboard mill sludge versus mixed culture bacteria for hydrogen production from paperboard mill wastewater.
A comparative evaluation of paperboard mill sludge (PMS) versus mixed culture bacteria (MCB) as inoculum for hydrogen production from paperboard mill wastewater (PMW) was investigated. The experiments were conducted at different initial cultivation pHs, inoculums to substrate ratios (ISRs gVS/gCOD), and hydraulic retention times (HRTs). The peak hydrogen yield (HY) of 5.29 ± 0.16 and 1.22 ± 0.11 mmol/gCODinitial was occurred at pH = 5 for MCB and PMS, respectively. At pH of 5, the HY and COD removal achieved the highest values of 2.26 ± 0.14 mmol/gCODinitial and 86 ± 1.6% at ISR = 6 for MCB, and 2.38 ± 0.25 mmol/gCODinitial and 60.4 ± 2.5% at ISRs = 3 for PMS. The maximum hydrogen production rate was 93.75 ± 8.9 mmol/day at HRT = 9.6 h from continuous upflow anaerobic reactor inoculated with MCB. Meanwhile, the 16S ribosomal RNA (rRNA) gene fragments indicated a dominance of a novel hydrogen-producing bacterium of Stenotrophomonas maltophilia for PMS microbial community. On the other hand, Escherichia fergusonii and Enterobacter hormaechei were the predominant species for MCB.
Environmental Engineering Department, Egypt-Japan University of Science and Technology (E-JUST), P.O. Box 179, New Borg El Arab City, Postal Code 21934, Alexandria, Egypt. ahmed.abdelwahhab@ejust.edu.eg.
Ahmed
A
Farghaly
Environmental Engineering Department, Egypt-Japan University of Science and Technology (E-JUST), P.O. Box 179, New Borg El Arab City, Postal Code 21934, Alexandria, Egypt.
Ahmed
A
Tawfik
Botany and Microbiology Department, Faculty of Science, Assiut University, 71516, Assiut, Egypt.
Amal
A
Danial
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology; D006859:Hydrogen / Q000032:analysis* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010209:Paper; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*
D006859:Hydrogen; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
Hydrogen; Mixed culture bacteria; Paperboard mill sludge; Paperboard mill wastewater; Stenotrophomonas maltophilia