Coordination of planar cell polarity pathways through Spiny-legs.
Morphogenesis and physiology of tissues and organs requires planar cell polarity (PCP) systems that orient and coordinate cells and their behaviors, but the relationship between PCP systems has been controversial. We have characterized how the Frizzled and Dachsous-Fat PCP systems are connected through the Spiny-legs isoform of the Prickle-Spiny-legs locus. Two different components of the Dachsous-Fat system, Dachsous and Dachs, can each independently interact with Spiny-legs and direct its localization in vivo. Through characterization of the contributions of Prickle, Spiny-legs, Dachsous, Fat, and Dachs to PCP in the Drosophila wing, eye, and abdomen, we define where Dachs-Spiny-legs and Dachsous-Spiny-legs interactions contribute to PCP, and provide a new understanding of the orientation of polarity and the basis of PCP phenotypes. Our results support the direct linkage of PCP systems through Sple in specific locales, while emphasizing that cells can be subject to and must ultimately resolve distinct, competing PCP signals.
Howard Hughes Medical Institute, Rutgers University, Piscataway, United States.|Waksman Institute of Microbiology, Rutgers University, Piscataway, United States.|Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, United States.
Abhijit A
AA
Ambegaonkar
Howard Hughes Medical Institute, Rutgers University, Piscataway, United States.|Waksman Institute of Microbiology, Rutgers University, Piscataway, United States.|Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, United States.
Kenneth D
KD
Irvine
D000825:Animal Structures / Q000502:physiology; D000818:Animals; D016764:Cell Polarity; D004330:Drosophila / Q000502:physiology*; D053263:Gene Regulatory Networks; D056426:Genetic Loci
D. melanogaster; cell biology; developmental biology; fat; pcp; polarity; prickle; stem cells