Gamma glutamyl transferase is an independent determinant for the association of insulin resistance with nonalcoholic fatty liver disease in Bangladeshi adults: Association of GGT and HOMA-IR with NAFLD.
OBJECTIVE
Nonalcoholic fatty liver disease (NAFLD) is a major cause of liver-related morbidity and is frequently associated with insulin resistance (HOMA-IR) syndrome. Recently serum gamma glutamyl transferase (GGT) has been considered as surrogate marker of NAFLD leading to oxidative stress and hepatocellular damage. In the present study we examined the association of serum GGT and HOMA-IR with NAFLD in Bangladeshi adult subjects.


METHODS
Under a cross-sectional analytical design a total of 110 subjects were recruited who came for their routine health check up in the BIHS Hospital, Darussalam, Dhaka, Bangladesh. After whole abdomen ultrasonography, 62 were diagnosed as non-NAFLD and 48 were NAFLD subjects. Serum glucose was measured by glucose-oxidase method, lipid profile and liver enzymes by enzymatic colorimetric method, glycosylated hemoglobin (HbA1c) was measured by high performance liquid chromatography (HPLC), serum insulin were measured by enzyme-linked immunosorbent assay. HOMA-IR was calculated by homeostasis model assessment (HOMA).


RESULTS
NAFLD subjects had significantly higher levels of GGT and HOMA-IR as compared to their non-NAFLD counterparts. Multiple linear regression analysis showed a significant positive association of HOMA-IR with GGT after adjusting the effects of waist circumference (WC) and HbA1c. In binary logistic regression analysis, HOMA-IR and GGT were found to be significant determinants of NAFLD after adjusting the effects of WC and HbA1c.


CONCLUSIONS
These results suggest that elevated levels of GGT and insulin resistance are more likely to develop NAFLD and thus support a role of these determinants in the pathogenesis of NAFLD in Bangladeshi adult subjects.
Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences, Dhaka, Bangladesh. Electronic address: israt.ru84@gmail.com.
Israt Ara
IA
Hossain
Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh.
Md Mijanur
MM
Rahman Shah
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Mohammad Khalilur
MK
Rahman
Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences, Dhaka, Bangladesh.
Liaquat
L
Ali
D000005:Abdomen / Q000000981:diagnostic imaging; D000328:Adult; D001459:Bangladesh; D001786:Blood Glucose / Q000032:analysis; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000000981:diagnostic imaging / Q000378:metabolism*; D014463:Ultrasonography; D055105:Waist Circumference; D005723:gamma-Glutamyltransferase / Q000097:blood*
D001786:Blood Glucose; D007328:Insulin; D005723:gamma-Glutamyltransferase
Gamma glutamyl transferase (GGT); Homeostasis model assessment insulin resistance (HOMA-IR); Nonalcoholic fatty liver disease (NAFLD)