Sensitive Trimodal Magnetic Resonance Imaging-Surface-Enhanced Resonance Raman Scattering-Fluorescence Detection of Cancer Cells with Stable Magneto-Plasmonic Nanoprobes.
Novel magneto-plasmonic nanoprobes were designed for multimodal diagnosis of cancer by combination of magnetic resonance imaging (MRI), surface-enhanced resonance Raman scattering (SERRS), and fluorescence emission in the very near infrared (VNIR). A controlled electrostatic assembly of silver nanoparticles (AgNPs), superparamagnetic iron oxide nanoparticles (SPIONs), VNIR dye Nile Blue (NB), and biopolymer chitosan (Chi) was used to formulate the AgIONs-Chi nanoprobes. The formulation protocol did not involve organic solvents and was rapid and efficient as confirmed by magnetic sorting. The SERRS response of the nanoprobes was very intense and constant for days. It decreased linearly upon 1000-fold dilution and was still recognizable at 0.1 nM NB concentration. After 30 days of storage, the SERRS loss was less than 30% and the hydrodynamic size of the AgIONs-Chi in PBS remained below 200 nm. The gradual decrease of the ratio SERRS/fluorescence allowed one to monitor the release of the fluorescent molecule upon long-term nanoprobe dissociation. The AgIONs-Chi exhibited 2-fold higher MRI contrast than that of commercially available SPION suspensions. Finally, the nanoprobes were actively uptaken by HeLa cancer cells and ensured trimodal MRI-SERRS-fluorescence detection of 10 μL cell inclusions in cm-sized agarose gels used here as phantom models of microtumors. The above results show that the magneto-plasmonic AgIONs-Chi are promising substrates for SERRS analysis in solution and for multimodal imaging of cancer cells.
Université François Rabelais, EA6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37 200 Tours, France.|CNRS-UPR 4301 - Centre de Biophysique Moléculaire - Equipe Complexes métalliques et IRM pour les applications biomédicales, rue Charles Sadron, 45 071 Orléans, France.
Ambre
A
Carrouée
Université François Rabelais, EA6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37 200 Tours, France.
Emilie
E
Allard-Vannier
CNRS-UPR 4301 - Centre de Biophysique Moléculaire - Equipe Complexes métalliques et IRM pour les applications biomédicales, rue Charles Sadron, 45 071 Orléans, France.
Sandra
S
Même
CNRS-UPR 4301 - Centre de Biophysique Moléculaire - Equipe Complexes métalliques et IRM pour les applications biomédicales, rue Charles Sadron, 45 071 Orléans, France.
Frederic
F
Szeremeta
CNRS-UPR 4301 - Centre de Biophysique Moléculaire - Equipe Complexes métalliques et IRM pour les applications biomédicales, rue Charles Sadron, 45 071 Orléans, France.
Jean-Claude
JC
Beloeil
Université François Rabelais, EA6295 Nanomédicaments et Nanosondes, 31 avenue Monge, 37 200 Tours, France.
Igor
I
Chourpa
D002469:Cell Separation / Q000379:methods*; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D060526:Magnetic Fields; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000473:pathology*; D010316:Particle Size; D059010:Single-Cell Analysis; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D014407:Tumor Cells, Cultured
D058185:Magnetite Nanoparticles