Cyclin D1 in pediatric neuroblastic tumors: A microarray analysis.
Neuroblastoma is the most common extracranial solid childhood tumor, which is believed to originate from primitive neuroblasts giving rise to the sympathetic nervous system. It was previously shown that cyclin D1 (CCDN1) in pediatric neuroblastic tumors (neuroblastoma, ganglioneuroblastoma, and ganglioneuroma) recapitulates its expression during the development of peripheral sympathetic nervous system (PSNS). In the present study, we performed a microarray analysis in order to evaluate the expression of cyclin D1 in neuroblastoma as compared to ganglioneuroma and ganglioneuroblastoma. We first confirmed that comparable levels of cyclin D1 are present in neuroblastoma and fetal neuroblasts. In addition, we observed that neuroblastoma is associated to significantly higher levels of cyclin D1 as compared to both ganglioneuroma and ganglioneuroblastoma. No differences are instead observable between ganglioneuroblastoma and ganglioneuroma. Finally, bioinformatic analysis of cyclin D1-functionally related genes, identified cyclin D2 as an additional marker/etiopathogenic factor in the development of neuroblastoma.
Department of Biomedical Sciences, School of Medicine, University of Catania, Catania, Italy.
Paolo
P
Fagone
Department of Biomedical Sciences, School of Medicine, University of Catania, Catania, Italy.
Ferdinando
F
Nicoletti
Department of Medical and Surgical Sciences and Advanced Technologies, G.F. Ingrassia, Azienda Ospedaliero-Universitaria "Policlinico-Vittorio Emanuele", Anatomic Pathology Section, School of Medicine, University of Catania, Catania, Italy.
Giada Maria
GM
Vecchio
Department of Biomedical and Biotechnological Sciences, Section of Physiology, School of Medicine, University of Catania, Catania, Italy.
Rosalba
R
Parenti
Department of Medical and Surgical Sciences and Advanced Technologies, G.F. Ingrassia, Azienda Ospedaliero-Universitaria "Policlinico-Vittorio Emanuele", Anatomic Pathology Section, School of Medicine, University of Catania, Catania, Italy. Electronic address: g.magro@unict.it.
Gaetano
G
Magro
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002648:Child; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D018305:Ganglioneuroblastoma / Q000378:metabolism* / Q000473:pathology; D005729:Ganglioneuroma / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis
D014408:Biomarkers, Tumor; C530104:CCND1 protein, human; D019938:Cyclin D1
Bioinformatic analysis; Cyclin D1; Microarray; Neuroblastic tumors; Neuroblastoma