Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis.
Lipases (EC 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting applications of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol) transesterification. Entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. The present work reports the heterologous expression and biochemical characterization of a novel Beauveria bassiana lipase with potential for biodiesel production. The His-tagged B. bassiana lipase A (BbLA) was produced in Komagataella pastoris in buffered methanol medium (BMM) induced with 1% methanol at 30°C. Purified BbLA was activated with 0.05% Triton X-100 and presented optimum activity at pH 6.0 and 50°C. N-glycosylation of the recombinant BbLA accounts for 31.5% of its molecular weight. Circular dichroism and molecular modeling confirmed a structure composed of α-helix and β-sheet, similar to α/β hydrolases. Immobilized BbLA was able to promote transesterification reactions in fish oil, demonstrating potential for biodiesel production. BbLA was successfully produced in K. pastoris and shows potential use for biodiesel production by the ethanolysis reaction.
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Ana C
AC
Vici
Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Andrezza F
AF
da Cruz
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Fernanda D A
FD
Facchini
Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Caio C
CC
de Carvalho
Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Marita G
MG
Pereira
Departamento de Química, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Raquel
R
Fonseca-Maldonado
Departamento de Química, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Richard J
RJ
Ward
Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de los Alimentos, Consejo Superior de Investigaciones Cientificas, Madrid España.
Benevides C
BC
Pessela
Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de los Alimentos, Consejo Superior de Investigaciones Cientificas, Madrid España.
Gloria
G
Fernandez-Lorente
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília Brazil.
Fernando A G
FA
Torres
Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
João A
JA
Jorge
Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto Brazil.
Maria L T M
ML
Polizeli
Beauveria bassiana; biodiesel; ethanolysis; heterologous expression; lipase; lipase purification