Biocontrol of the Potato Blackleg and Soft Rot Diseases Caused by Dickeya dianthicola.
Development of protection tools targeting Dickeya species is an important issue in the potato production. Here, we present the identification and the characterization of novel biocontrol agents. Successive screenings of 10,000 bacterial isolates led us to retain 58 strains that exhibited growth inhibition properties against several Dickeya sp. and/or Pectobacterium sp. pathogens. Most of them belonged to the Pseudomonas and Bacillus genera. In vitro assays revealed a fitness decrease of the tested Dickeya sp. and Pectobacterium sp. pathogens in the presence of the biocontrol agents. In addition, four independent greenhouse assays performed to evaluate the biocontrol bacteria effect on potato plants artificially contaminated with Dickeya dianthicola revealed that a mix of three biocontrol agents, namely, Pseudomonas putida PA14H7 and Pseudomonas fluorescens PA3G8 and PA4C2, repeatedly decreased the severity of blackleg symptoms as well as the transmission of D. dianthicola to the tuber progeny. This work highlights the use of a combination of biocontrol strains as a potential strategy to limit the soft rot and blackleg diseases caused by D. dianthicola on potato plants and tubers.
Institute for Integrative Biology of the Cell, CNRS CEA Université Paris-Sud, Université Paris-Saclay, Saclay Plant Sciences, Gif-sur-Yvette, France Research, Development, Promotion of Seed Potatoes, French Federation of Seed Potato Growers, Paris, France.
Yannick
Y
Raoul des Essarts
Institute for Integrative Biology of the Cell, CNRS CEA Université Paris-Sud, Université Paris-Saclay, Saclay Plant Sciences, Gif-sur-Yvette, France Seeds Innovation Protection Research Environment, Comité Nord Plants de Pomme de Terre, Achicourt, France.
Jérémy
J
Cigna
Research, Development, Promotion of Seed Potatoes, French Federation of Seed Potato Growers, Paris, France UMR 1349 IGEPP INRA-Agrocampus Ouest Rennes, Le Rheu, France.
Angélique
A
Quêtu-Laurent
Seeds Innovation Protection Research Environment, Comité Nord Plants de Pomme de Terre, Achicourt, France.
Aline
A
Caron
Seeds Innovation Protection Research Environment, Comité Nord Plants de Pomme de Terre, Achicourt, France.
Euphrasie
E
Munier
Seeds Innovation Protection Research Environment, Comité Nord Plants de Pomme de Terre, Achicourt, France.
Amélie
A
Beury-Cirou
Research, Development, Promotion of Seed Potatoes, French Federation of Seed Potato Growers, Paris, France UMR 1349 IGEPP INRA-Agrocampus Ouest Rennes, Le Rheu, France valerie.helias@fnpppt.fr denis.faure@i2bc.paris-saclay.fr.
Valérie
V
Hélias
Institute for Integrative Biology of the Cell, CNRS CEA Université Paris-Sud, Université Paris-Saclay, Saclay Plant Sciences, Gif-sur-Yvette, France valerie.helias@fnpppt.fr denis.faure@i2bc.paris-saclay.fr.
Denis
D
Faure
D000898:Antibiosis; D061046:Biological Control Agents / Q000302:isolation & purification*; D004269:DNA, Bacterial; D004755:Enterobacteriaceae / Q000235:genetics / Q000254:growth & development*; D044043:Pectobacterium / Q000235:genetics / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D035281:Plant Tubers / Q000382:microbiology*; D011549:Pseudomonas / Q000502:physiology*; D011551:Pseudomonas fluorescens / Q000502:physiology; D016958:Pseudomonas putida / Q000502:physiology; D011198:Solanum tuberosum / Q000382:microbiology*
D061046:Biological Control Agents; D004269:DNA, Bacterial