Environmental Effects of Nanoceria on Seed Production of Common Bean (Phaseolus vulgaris): A Proteomic Analysis.
The rapidly growing literature on the response of edible plants to nanoceria has provided evidence of its uptake and bioaccumulation, which delineates a possible route of entry into the food chain. However, little is known about how the residing organic matter in soil may affect the bioavailability and resulting impacts of nanoceria on plants. Here, we examined the effect of nanoceria exposure (62.5-500 mg/kg) on kidney bean (Phaseolus vulgaris) productivity and seed quality as a function of soil organic matter content. Cerium accumulation in the seeds produced from plants in organic matter enriched soil showed a dose-dependent increase, unlike in low organic matter soil treatments. Seeds obtained upon nanoceria exposure in soils with higher organic matter were more susceptible to changes in nutrient quality. A quantitative proteomic analysis of the seeds produced upon nanoceria exposure provided evidence for upregulation of stress-related proteins at 62.5 and 125 mg/kg nanoceria treatments. Although the plants did not exhibit overt toxicity, the major seed proteins primarily associated with nutrient storage (phaseolin) and carbohydrate metabolism (lectins) were significantly down-regulated in a dose dependent manner upon nanoceria exposure. This study thus suggests that nanoceria exposures may negatively affect the nutritional quality of kidney beans at the cellular and molecular level. More confirmatory studies with nanoceria along different species using alternative and orthogonal "omic" tools are currently under active investigation, which will enable the identification of biomarkers of exposure and susceptibility.
Department of Chemistry, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.|University of California Center for Environmental Implications of Nanotechnology (UC CEIN) , El Paso, Texas United States.
Sanghamitra
S
Majumdar
Border Biomedical Research Center, Department of Biological Sciences, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.
Igor C
IC
Almeida
Border Biomedical Research Center, Department of Biological Sciences, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.
Emma A
EA
Arigi
Saw Swee Hock School of Public Health, National University of Singapore and National University Health System , Singapore.
Hyungwon
H
Choi
Border Biomedical Research Center, Department of Biological Sciences, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.
Nathan C
NC
VerBerkmoes
Department of Chemistry, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.
Jesica
J
Trujillo-Reyes
Autonomous University of Ciudad Juarez , Departamento de Química y Biología, Instituto de Ciencias Biomédicas, Anillo envolvente PRONAF y Estocolmo, Ciudad Juarez, Chihuahua 32310, México.
Juan P
JP
Flores-Margez
Department of Analytical Chemistry, The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, Connecticut 06504, United States.
Jason C
JC
White
Department of Chemistry, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.|Environmental Science and Engineering PhD Program, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.|University of California Center for Environmental Implications of Nanotechnology (UC CEIN) , El Paso, Texas United States.
Jose R
JR
Peralta-Videa
Department of Chemistry, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.|Environmental Science and Engineering PhD Program, The University of Texas at El Paso , 500 West University Ave., El Paso, Texas 79968, United States.|University of California Center for Environmental Implications of Nanotechnology (UC CEIN) , El Paso, Texas United States.
Jorge L
JL
Gardea-Torresdey
D050260:Carbohydrate Metabolism / Q000187:drug effects; D002563:Cerium / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D053758:Nanoparticles / Q000008:administration & dosage / Q000633:toxicity; D009753:Nutritive Value; D027805:Phaseolus / Q000187:drug effects* / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000187:drug effects* / Q000378:metabolism; D012989:Soil Pollutants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D014018:Tissue Distribution
D010940:Plant Proteins; D012989:Soil Pollutants; D002563:Cerium; C030583:ceric oxide