Fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria.
OBJECTIVE
This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (LAB) isolated from sugarcane grown in a Brazil on the quality of corn silage.


RESULTS
Three strains of Lactobacillus buchneri (UFLA SLM11, UFLA SLM103 and UFLA SLM108), five strains of Lactobacillus plantarum (UFLA SLM08, UFLA SLM41, UFLA SLM45, UFLA SLM46 and UFLA SLM105), and one strain of Leuconostoc mesenteroides (UFLA SLM06) were evaluated at 0, 10, 30, 60 and 90 day after inoculating corn forage. The inoculation of the LAB strains did not influence the chemical composition of the silage, but pH, acetic acid and 1,2-propanediol were affected by treatment. The silages inoculated with UFLA SLM11 and SLM108 contained the lowest yeast and filamentous fungi counts during fermentation. Bacteria belonging to the Enterobacteriaceae family, Clostridium genus were detected in the silages inoculated with Lact. buchneri UFLA SLM 11, 103 and 108, as shown by DGGE analysis. Silages inoculated with Lact. buchneri UFLA SLM 11 showed higher aerobic stability.


CONCLUSIONS
The Lact. buchneri UFLA SLM11 strain was considered promising as a starter culture or inoculant for corn silages.


CONCLUSIONS
The selection of microbial inoculants for each crop promotes improvement of silage quality. Studies on the chemical and microbiological characteristics of silage provide useful information for improving ensiling techniques.
Department of Biology, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
A O
AO
Santos
Department of Animal Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
C L S
CL
Ávila
Department of Animal Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
J C
JC
Pinto
Department of Animal Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
B F
BF
Carvalho
Department of Food Science, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
D R
DR
Dias
Department of Biology, Federal University of Lavras, Lavras, Minas Gerais, Brazil.
R F
RF
Schwan
D000332:Aerobiosis; D044822:Biodiversity; D001938:Brazil; D005285:Fermentation; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D007777:Lactobacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D031786:Saccharum / Q000382:microbiology; D012820:Silage / Q000032:analysis / Q000382:microbiology*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
D019344:Lactic Acid
DGGE; Enterobacteriaceae; Lactobacillus buchneri; aerobic stability; lactic acid bacteria