Early Effect of Bariatric Surgery on the Circadian Rhythms of Adipokines in Morbidly Obese Women.
BACKGROUND
The circadian pattern of adipokines is blunted in obese subjects, and we tested the hypothesis that bariatric surgery could normalize the 24-hr pattern of adipokines. Therefore, this study was designed to examine the early impact of the newly designed sleeve gastrectomy with transit bipartition (SGTB) surgery on the circadian pattern of leptin, adiponectin, and resistin in morbidly obese subjects.


METHODS
The study group included six morbidly obese women [body mass index (BMI) 41.3 ± 1.53 kg/m(2)] who underwent SGTB and four lean women (BMI 18.61 ± 0.92 kg/m(2)). Blood from all subjects was collected before and 3 months after bariatric surgery every 6 hr throughout the 24-hr period. The circadian pattern of leptin, adiponectin, and resistin was measured by enzyme-linked immunosorbent assay or Luminex techniques.


RESULTS
Lean women exhibited rise of plasma leptin levels at nighttime, whereas obese women had an increase in the overall plasma leptin levels throughout the 24-hr period, lacking the physiological rise of nocturnal leptin levels compared to controls. Obese women had a decrease in 24-hr adiponectin levels and similar plasma resistin levels compared to controls. Three months after SGTB, obese women lost 16.0% (P < 0.005) of their initial body weight and had a decrease in overall 24-hr leptin levels. However, there was no recovery of the nocturnal rise in leptin levels 3 months after SGTB. The 24-hr adiponectin levels were still decreased after SGTB surgery compared to controls, while resistin levels were decreased only during night time after SGTB.


CONCLUSIONS
These results suggested that SGTB is an efficient innovative procedure to rapidly decrease 24-hr leptin levels. However, after 3 months, SGTB was not enough to recover the physiological nocturnal rise of leptin levels present in lean subjects.
1 Department of Surgery, Universidade Federal do Paraná-UFPR , Curitiba, Brazil .
José Fabiano
JF
Costa Justus
1 Department of Surgery, Universidade Federal do Paraná-UFPR , Curitiba, Brazil .
Antonio Carlos
AC
Ligocki Campos
2 Diabetes and Obesity Research Laboratory , IDIBAPS, Barcelona, Spain .
Ana Lucia C
AL
Figueroa
2 Diabetes and Obesity Research Laboratory , IDIBAPS, Barcelona, Spain .|3 CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) , Madrid, Spain .
Ramon
R
Gomis
4 Hospital das Clinicas Faculdade de Medicina, Universidade de São Paulo-USP , Sao Paulo, Brazil .
Marco Aurélio
MA
Santo
5 Department of Biology, Universidade Estadual de Ponta Grossa , Ponta Grossa, Brazil .
Giovani Marino
GM
Fávero
6 Instituto de Pesquisa Aplicada em Medicina-INSPAM , Ponta Grossa, Brazil .
Fábio Quirillo
FQ
Milléo
3 CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM) , Madrid, Spain .|7 Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá/UEM , Maringá, Brazil .
Elaine
E
Vieira
D054392:Adipokines / Q000097:blood*; D052242:Adiponectin / Q000097:blood; D000328:Adult; D050110:Bariatric Surgery / Q000379:methods*; D015992:Body Mass Index; D016022:Case-Control Studies; D002940:Circadian Rhythm; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005743:Gastrectomy / Q000379:methods*; D006801:Humans; D020738:Leptin / Q000097:blood; D009767:Obesity, Morbid / Q000097:blood / Q000175:diagnosis / Q000503:physiopathology / Q000601:surgery*; D052243:Resistin / Q000097:blood; D013997:Time Factors; D016896:Treatment Outcome; D055815:Young Adult
C499918:ADIPOQ protein, human; D054392:Adipokines; D052242:Adiponectin; D020738:Leptin; C499624:RETN protein, human; D052243:Resistin