Ferulic acid ethyl ester diminished Complete Freund's Adjuvant-induced incapacitation through antioxidant and anti-inflammatory activity.
Ferulic acid ethyl ester (FAEE) is a derivate from ferulic acid which reportedly has antioxidant effect; however, its role on inflammation was unknown. In this study, we investigated the orally administered FAEE anti-inflammatory activity on experimental inflammation models and Complete Freund's Adjuvant (CFA)-induced arthritis in rats. CFA-induced arthritis has been evaluated by incapacitation model and radiographic knee joint records at different observation time. FAEE (po) reduced carrageenan-induced paw edema (p < 0.001) within the 1st to 5th hours at 50 and 100 mg/kg doses. FAEE 50 and 100 mg/kg, po inhibited leukocyte migration into air pouch model (p < 0.001), and myeloperoxidase, superoxide dismutase, and catalase activities (p < 0.001) increased total thiol concentration and decreased the TNF-α and IL-1β concentrations, NO, and thiobarbituric acid reactive species. In the CFA-induced arthritis, FAEE 50 and 100 mg/kg significantly reduced the edema and the elevation paw time, a joint disability parameter, since second hour after arthritis induction (p < 0.001). FAEE presented rat joint protective activity in radiographic records (p < 0.001). The data suggest that the FAEE exerts anti-inflammatory activity by inhibiting leukocyte migration, oxidative stress reduction, and pro-inflammatory cytokines.
Francisco Valmor  Macedo
FV
Cunha
Bruno de Sousa
Bde S
Gomes
Benedito de Sousa
Bde S
Neto
Alana Rodrigues
AR
Ferreira
Damião Pergentino
DP
de Sousa
Maria do Carmo
Mdo C
de Carvalho e Martins
Francisco de Assis
Fde A
Oliveira
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002109:Caffeic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D002351:Carrageenan; D002374:Catalase / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D005620:Freund's Adjuvant; D005978:Glutathione / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D007719:Knee Joint / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D009573:Nitrites / Q000378:metabolism; D011859:Radiography; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D002109:Caffeic Acids; D053583:Interleukin-1beta; D009573:Nitrites; D017392:Thiobarbituric Acid Reactive Substances; D014409:Tumor Necrosis Factor-alpha; C099085:ethyl ferulate; D002351:Carrageenan; D005620:Freund's Adjuvant; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione