Protective action of the phyllanthin against carbon  tetrachloride-induced hepatocyte damage in Cyprinus carpio.
The present study was to investigate the protective effect and possible mechanism of phyllanthin against carbon tetrachloride (CCl4)-induced hepatocyte damage in carp. Phyllanthin (5, 10, and 15 μg/ml) was added to carp primary hepatocytes before (pre-treatment) and after (post-treatment) incubation of the hepatocytes in medium containing CCl4 at 8 mM; supernatant and cell were collected for the analyses of cell viability, biochemical parameters, and gene expression. The results showed that phyllanthin at the concentration of 15 μg/ml significantly suppressed the elevation of glutamate pyruvate transaminase (GPT), glutamate oxalate transaminase (GOT), lactate dehydrogenase (LDH), and malondialdehyde (MDA), and the reduction of cell viability, superoxide dismutase (SOD) activity, cytochrome P450 1a (CYP1A), and cytochrome P450 3a (CYP3A) messenger RNA (mRNA) levels expect LDH in the post-treatment. The levels of GPT, GOT, and CYP1A mRNA were also effectively restored in the pretreatment with phyllanthin (10 μg/ml). Overall, our results suggested that phyllanthin may be used as a hepatoprotective agent to prevent liver diseases in fish.
Rui
R
Jia
Jin-Liang
JL
Du
Li-Ping
LP
Cao
Ying-Juan
YJ
Liu
Pao
P
Xu
Guo-Jun
GJ
Yin
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D002347:Carps / Q000293:injuries / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D005393:Fish Diseases / Q000188:drug therapy* / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D020011:Protective Agents / Q000494:pharmacology*
D017705:Lignans; D020011:Protective Agents; C517985:phyllanthin; D002251:Carbon Tetrachloride