Characterization of Sugarcane Mosaic Virus Scmv1 and Scmv2 Resistance Regions by Regional Association Analysis in Maize.
Sugarcane Mosaic Virus (SCMV) causes one of the most severe virus diseases in maize worldwide, resulting in reduced grain and forage yield in susceptible cultivars. In this study, two association panels consisting of 94 inbred lines each, from China and the U.S., were characterized for resistance to two isolates: SCMV-Seehausen and SCMV-BJ. The population structure of both association panels was analyzed using 3072 single nucleotide polymorphism (SNP) markers. The Chinese and the U.S. panel were both subdivided into two sub-populations, the latter comprised of Stiff Stalk Synthetic (SS) lines and Non Stiff Stalk Synthetic (NSS). The relative kinships were calculated using informative 2947 SNPs with minor allele frequency ≥ 5% and missing data ≤ 20% for the Chinese panel and 2841 SNPs with the same characteristics were used for the U.S. panel. The Scmv1 region was genotyped using 7 single sequence repeat (SSR) and sequence-tagged site (STS) markers, and 12 SSR markers were used for the Scmv2 region in the U.S. panel, while 5 of them were used for the Chinese panel. For all traits, a MLM (Mix Linear Model) controlling both population structure and relative kinship (Q + K) was used for association analysis. Three markers Trx-1, STS-11, and STS-12 located in the Scmv1 region were strongly associated (P = 0.001) with SCMV resistance, and explained more than 16.0%, 10.6%, and 19.7% of phenotypic variation, respectively. 207FG003 located in the Scmv2 region was significantly associated (P = 0.001) with SCMV resistance, and explained around 18.5% of phenotypic variation.
National Maize Improvement Center, China Agricultural University, Beijing, 100094, China; Department of Agronomy, Iowa State University, Ames, Iowa, 50011, United States of America.
Pengfei
P
Leng
Department of Agronomy, Iowa State University, Ames, Iowa, 50011, United States of America.
Qing
Q
Ji
National Maize Improvement Center, China Agricultural University, Beijing, 100094, China.
Yongfu
Y
Tao
Department of Agronomy, Iowa State University, Ames, Iowa, 50011, United States of America.
Rania
R
Ibrahim
Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Guangtang
G
Pan
National Maize Improvement Center, China Agricultural University, Beijing, 100094, China.
Mingliang
M
Xu
Department of Agronomy, Iowa State University, Ames, Iowa, 50011, United States of America.
Thomas
T
Lübberstedt
D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics* / Q000276:immunology; D017343:Genes, Plant; D005819:Genetic Markers / Q000235:genetics; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017800:Potyvirus / Q000276:immunology*; D016324:Sequence Tagged Sites; D003313:Zea mays / Q000235:genetics* / Q000276:immunology* / Q000821:virology
D005819:Genetic Markers