A Magnetic Nanoparticle Based Enzyme-Linked Immunosorbent Assay for Sensitive Quantification of Zearalenone in Cereal and Feed Samples.
A novel enzyme-linked immunosorbent assay based on magnetic nanoparticles and biotin/streptavidin-HRP (MNP-bsELISA) was developed for rapid and sensitive detection of zearalenone (ZEN). The detection signal was enhanced and the sensitivity of the assay was improved by combined use of antibody-conjugated magnetic nanoparticles and biotin-streptavidin system. Under the optimized conditions, the regression equation for quantification of ZEN was y = -0.4287x + 0.3132 (R² = 0.9904). The working range was 0.07-2.41 ng/mL. The detection limit was 0.04 ng/mL and IC50 was 0.37 ng/mL. The recovery rates of intra-assay and inter-assay ranged from 92.8%-111.9% and 91.7%-114.5%, respectively, in spiked corn samples. Coefficients of variation were less than 10% in both cases. Parallel analysis of cereal and feed samples showed good correlation between MNP-bsELISA and liquid chromatograph-tandem mass spectrometry (R² = 0.9283). We conclude that this method is suitable for rapid detection of zearalenone in cereal and feed samples in relevant laboratories.
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China. zhangian073@163.com.
Xian
X
Zhang
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China. wangxin20095017@126.com.
Xin
X
Wang
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China. sunmengjiao265@126.com.
Mengjiao
M
Sun
Food Safety Key Laboratory of Zhejiang Province, Zhejiang Entry-Exit Inspection and Quarantine Bureau, 126 Fuchun Road, Hangzhou 310012, China. zxf@ziq.gov.cn.
Xiaofeng
X
Zhang
College of Animal Science and Technology, Zhejiang A&F University, 88 Huanbei Road, Lin'an 311300, China. songhh@zafu.edu.cn.
Houhui
H
Song
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China. yanyaxian@sjtu.edu.cn.
Yaxian
Y
Yan
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China. sunjhe@sjtu.edu.cn.
Jianhe
J
Sun
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China. xlli@zju.edu.cn.
Xiaoliang
X
Li
Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, China. whfang@zju.edu.cn.
Weihuan
W
Fang
D000911:Antibodies, Monoclonal / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
D000911:Antibodies, Monoclonal; D058185:Magnetite Nanoparticles; D012710:Serum Albumin, Bovine; D015025:Zearalenone; D001710:Biotin; D019809:Streptavidin
biotin-streptavidin; immunoassay; magnetic nanoparticles; quantification; zearalenone