A Novel Ribonuclease from Rana Chensinensis and Its Potential for the Treatment of Human Breast Cancer.
Onconase, a member of the pancreatic RNAase A superfamily of ribonucleases, is a chemotherapeutic agent, which has demonstrated selective antitumor activity in a variety of human malignancies. However, little is known about the mechanisms of it's action on human breast cancer cells. To investigate a novel Onconase from the frog of Rana chensinensis changbaishanensis on human breast cancer cells and the underlying mechanisms, a novel Onconase named Rdchonc from Rana chensinensis changbaishanensis was cloned by polymerase chain reaction. SDS-PAGE revealed that the Rdchonc had a high heterologous expression in Escherichia coli BL21(DE3). The MTT assay indicated that purified Rdchonc was cytotoxic to human breast cancer MCF-7 and MD-MB-231 cells. Treatment with 20 μg/mL Rdchonc protein significantly reduced the invasive capacities of MCF-7 and MD-MB-231 cells. Interestingly, the authors found that such inhibitory effort on tumor cell growth induced by Rdchonc treatment may be explained by the regulation of proapoptotic Bcl-2 family proteins and inhibition of MEK/ERK phosphorylation.
1 College of Chemistry, Jilin University , Changchun, People's Republic of China .|2 Laboratory of Nutrition and Functional Food, Jilin University , Changchun, People's Republic of China .
Zuozhao
Z
Wang
1 College of Chemistry, Jilin University , Changchun, People's Republic of China .
Feng
F
Lin
2 Laboratory of Nutrition and Functional Food, Jilin University , Changchun, People's Republic of China .
Jingbo
J
Liu
1 College of Chemistry, Jilin University , Changchun, People's Republic of China .
Fangping
F
Qiu
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D011898:Ranidae / Q000378:metabolism*; D012260:Ribonucleases / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D012260:Ribonucleases
Bcl-2; MEK/ERK; Onconase; human breast cancer