Candidate Gene and MicroRNA Expression in Fetal Membranes and Preterm Delivery Risk.
We investigated candidate gene and microRNA (miRNA) expression in amnion and chorion in relation to risk of preterm delivery (PTD). Amnion and chorion were separated from placenta and collected at delivery from participants who delivered at term (N = 10) and from participants who delivered preterm following spontaneous labor (sPTL-PTD; N = 10), premature rupture of membranes (PPROM-PTD; N = 10), and preeclampsia (PE-PTD; N = 10). Expression of genes (metalloproteinase [MMP] 2, MMP-9, and tissue inhibitors of MMP-1) and miRNAs (miR-199a*, -202*, -210, -214, -223, and -338) was profiled using quantitative real-time polymerase chain reaction approaches. Adjusted multinomial logistic regression models were used to calculate relative risk ratios (RRR), 95% confidence intervals, and P values. Among controls, the expression of miR-199a*, -202*, and -214 was lower in the amnion compared with their expression in the chorion, whereas the expression of miR-210 was higher in the amnion compared with its expression in the chorion (all P values < .05). In the amnion, MMP-9 expression was associated with PTD risk (overall P value = .0092), and MMP-9 expression was positively associated with the risk of PPROM-PTD (RRR: 31.10) and inversely associated with the risk of PE-PTD (RRR:6.55e-6), although individual associations were not statistically significant. In addition, in the amnion, the expression of miR-210 (RRR: 0.45; overall P value = .0039) was inversely associated with the risk of PE-PTD, and miR-223 was inversely associated with all subtypes of PTD (overall P value = .0400). The amnion and chorion differ in their miRNA expression. The expression of MMP-9, miR-210, and -223 in the amnion is associated with PTD risk.
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA Department of Epidemiology, University of Washington, Seattle, WA, USA danenq@uw.edu.
Daniel A
DA
Enquobahrie
Department of Epidemiology, University of Washington, Seattle, WA, USA.
Mark
M
Hensley
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA.
Chunfang
C
Qiu
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA.
Dejene F
DF
Abetew
Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, USA.
Karin
K
Hevner
Department of Mathematics and Statistics, Georgetown University, Washington DC, USA.
Mahlet G
MG
Tadesse
Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, MA, USA.
Michelle A
MA
Williams
D000328:Adult; D000650:Amnion / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002823:Chorion / Q000378:metabolism*; D005321:Extraembryonic Membranes / Q000378:metabolism*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D047928:Premature Birth / Q000378:metabolism*; D012307:Risk Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism
D015415:Biomarkers; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D035683:MicroRNAs; D019715:Tissue Inhibitor of Metalloproteinase-1; C569392:MIRN202 microRNA, human; C543800:MIRN210 microRNA, human; C547397:MIRN214 microRNA, human; C525067:MIR223, human; C532050:MIRN338 microRNA, human; C560822:TIMP1 protein, human; C531408:mirn199 microRNA, human; C522361:MMP2 protein, human; C579270:MMP9 protein, human
amnion; chorion; gene expression; microRNA expression; preterm delivery