Intermittent administration of human parathyroid hormone (1-34) increases fixation of strontium-doped hydroxyapatite coating titanium implants via electrochemical deposition in ovariectomized rat femur.
Previous studies have demonstrated the effect of human parathyroid hormone (1-34) (PTH) or strontium-doped hydroxyapatite coating (Sr-HA) on osteoporotic bone implantation. However, reports about effects of PTH plus Sr-HA on bone osseointegration of titanium implants in a state of osteoporosis were limited. This study was designed to investigate the effects of intermittent administration of human parathyroid hormone (1-34) on strontium-doped hydroxyapatite coating (Sr-HA) implant fixation in ovariectomized (OVX) rats. Twelve weeks after bilateral ovariectomy, all animals were randomly divided into four groups including control group, Sr group, PTH group and PTH+Sr group. Forty OVX rats accepted implant insertion in the distal femurs, control group, and PTH group with HA implants and the Sr group and PTH+Sr group with Sr-HA implants. Animals from PTH group and PTH+Sr group then randomly received PTH (60 µg/kg, 3 times a week) until death at 12 weeks. After 12-week healing period, implants from group PTH+Sr revealed improved osseointegration compared with other treatment groups, which is manifested by the exceeding increase of bone area ratio and bone-to-implant contact, the trabecular microarchitecture and the maximal push-out force displayed by tests like histomorphometry, micro-CT, and biomechanics evaluation. These results demonstrated that PTH+ Sr-HA coatings could enhance implant osseointegration in OVX rats, and suggested the feasibility of using this method to improve implant fixation in osteoporotic bone.
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Zhou-Shan
ZS
Tao
Endocrine & Metabolic Diseases Unit, Affiliated Hospital of Guizhou Medcial University, Guizhou, PR China.
Wan-Shu
WS
Zhou
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Zhou
Z
Qiang
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Kai-kai
KK
Tu
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Zheng-Liang
ZL
Huang
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Hong-Ming
HM
Xu
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China.
Tao
T
Sun
Department of Orthopaedic Surgery, Wenzhou Central Hospital, Wenzhou, Zhejiang, PR China.
Yang-Xun
YX
Lv
Sichuan Provincial Orthopedics Hospital, Sichuan, PR China.
Wei
W
Cui
Department of Orthopaedic Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang, PR China tzs19900327@163.com.
Lei
L
Yang
D000818:Animals; D001696:Biomechanical Phenomena; D050071:Bone Density Conservation Agents / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000473:pathology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D004563:Electrochemistry; D005260:Female; D005269:Femur / Q000473:pathology*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D016348:Osseointegration; D010024:Osteoporosis / Q000188:drug therapy / Q000503:physiopathology; D010281:Parathyroid Hormone / Q000008:administration & dosage* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013324:Strontium / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D055114:X-Ray Microtomography
D050071:Bone Density Conservation Agents; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; C470125:PTH protein, human; D010281:Parathyroid Hormone; C076185:strontium hydroxyapatite; D017886:Durapatite; D014025:Titanium; D013324:Strontium
Osteoporosis; hydroxyapatite-coated titanium implants; osseointegration; parathyroid hormone (1-34); strontium