Human papillomavirus type 2 associated with pyogenic granuloma in patients without clinical evidence of warts.
BACKGROUND
Pyogenic granuloma is a non-neoplastic lesion that frequently occurs in the skin and mucous membranes of children and pregnant women. The anatomical sites of pyogenic granulomas overlap with those of wart infections caused by the human papillomavirus (HPV).


OBJECTIVE
This study assessed the presence of HPV DNA in pyogenic granuloma samples by polymerase chain reaction.


METHODS
Eighteen pyogenic granuloma biopsies from patients without a clinical history or evidence of verruca in the studied area were tested for the presence of the HPV genome. The presence of HPV DNA was screened by three independent polymerase chain reaction reactions using standard consensus primer sets targeted to the L1 or E1 consensus regions of HPV genome. The HPV DNA-positive samples were genotyped using methodologies enabling the identification of up to 30 HPVs, including oncogenic, nononcogenic, and cutaneous viral types.


RESULTS
The HPV DNA was detected in 44.4% (eight of 18) of the samples, with HPV-2 being the only type in the eight HPV DNA-positive samples. Contamination with HPV-2 sequences throughout the entire process was reliably eliminated.


CONCLUSIONS
This report is the first to suggest an association between HPV-2 and pyogenic granuloma. This relationship is similar to that observed between HPV-2 and nongenital warts.
Servicio de Dermatología, Hospital Universitario "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Osvaldo T
OT
Vázquez-Martínez
Laboratorio Integrativo en Biología Molecular y Celular. Centro de Investigaciones Odontológicas, Facultad de Odontología, Universidad de los Andes, Mérida, Venezuela.
Anajulia
A
González-Betancourt
Servicio de Anatomía Patológica y Citopatología, Hospital Universitario "José Eleuterio González", Universidad Autónoma de Nuevo León, Monterrey, Mexico.|Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
María Carmen
MC
Barboza-Cerda
Servicio de Dermatología, Hospital Universitario "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Sergio E
SE
González-González
Centro Mesoamericano de Estudios en Salud Pública y Desastres (CEMESAD), Universidad Autónoma de Chiapas (UNACH), Tapachula, Chiapas, Mexico.
Ángel
Á
Lugo-Trampe
Servicio de Dermatología, Hospital Universitario "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Oliverio
O
Welsh
Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.|Laboratorio de Genética Molecular, Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Augusto
A
Rojas-Martínez
Laboratorio de Genética Molecular, Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Herminia G
HG
Martínez-Rodríguez
Servicio de Dermatología, Hospital Universitario "Dr. José E. González", Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Jorge
J
Ocampo-Candiani
Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.|Laboratorio de Genética Molecular, Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.
Rocío
R
Ortiz-López
D000293:Adolescent; D000328:Adult; D001132:Arm; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D005838:Genotype; D017789:Granuloma, Pyogenic / Q000821:virology*; D006257:Head; D006801:Humans; D008297:Male; D008875:Middle Aged; D009333:Neck; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013909:Thorax; D055815:Young Adult
D004279:DNA, Viral