Screening and identification of five serum proteins as novel potential biomarkers for cured pulmonary tuberculosis.
Rapid and efficient methods for the determination of cured tuberculosis (TB) are lacking. A total of 85 differentially expressed serum proteins were identified by iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) analysis (fold change >1.50 or <0.60, P < 0.05). We validated albumin (ALB), Rho GDP-dissociation inhibitor 2 (ARHGDIB), complement 3 (C3), ficolin-2 (FCN2), and apolipoprotein (a) (LPA) using the enzyme-linked immunosorbent assay (ELISA) method. Significantly increased ALB and LPA levels (P = 0.036 and P = 0.012, respectively) and significantly reduced ARHGDIB, C3, and FCN2 levels (P < 0.001, P = 0.035, and P = 0.018, respectively) were observed in cured TB patients compared with untreated TB patients. In addition, changes in ALB and FCN2 levels occurred after 2 months of treatment (P < 0.001 and P = 0.030, respectively). We established a cured TB model with 87.10% sensitivity, 79.49% specificity, and an area under the curve (AUC) of 0.876. The results indicated that ALB, ARHGDIB, C3, FCN2, and LPA levels might serve as potential biomarkers for cured TB. Our study provides experimental data for establishing objective indicators of cured TB and also proposes potential markers for evaluating the efficacy of anti-TB drugs.
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Chong
C
Wang
Department of Respiratory Medicine, The Sixth Hospital of Shaoxing, Shaoxing 312000, P.R. China.
Li-Liang
LL
Wei
Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, P.R. China.
Li-Ying
LY
Shi
Department of Tuberculosis, The First Hospital of Jiaxing, Jiaxing 314001, P.R. China.
Zhi-Fen
ZF
Pan
Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, P.R. China.
Xiao-Mei
XM
Yu
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Tian-Yu
TY
Li
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Chang-Ming
CM
Liu
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Ze-Peng
ZP
Ping
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Ting-Ting
TT
Jiang
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Zhong-Liang
ZL
Chen
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Lian-Gen
LG
Mao
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Zhong-Jie
ZJ
Li
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, P.R. China.
Ji-Cheng
JC
Li
D000293:Adolescent; D000328:Adult; D000368:Aged; D000995:Antitubercular Agents / Q000627:therapeutic use; D001054:Apolipoproteins A / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002853:Chromatography, Liquid; D003176:Complement C3 / Q000032:analysis*; D018572:Disease-Free Survival; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D037102:Lectins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis; D012372:ROC Curve; D012709:Serum Albumin / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D016896:Treatment Outcome; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D055815:Young Adult; D062949:rho Guanine Nucleotide Dissociation Inhibitor beta / Q000097:blood*; D000097604:Ficolins
D000995:Antitubercular Agents; D001054:Apolipoproteins A; D015415:Biomarkers; D003176:Complement C3; D037102:Lectins; D012709:Serum Albumin; D062949:rho Guanine Nucleotide Dissociation Inhibitor beta; D000097604:Ficolins; C569658:ARHGDIB protein, human