Skeletal Muscle Response to Endurance Training in IL-6-/- Mice.
We examined effects of moderate-intensity endurance training on muscle COX/CS activities and V'O2max in control WT and IL-6(-/-) mice. Animals were exercised for 10 weeks on treadmill for 1 h, 5 days a week at velocity of 6 m·min(-1) which was increased by 0.5 m·min(-1) every 2 weeks up to 8 m·min(-1) . Training triggered an increase of enzyme activities in soleus muscle of WT mice (COX: 480.3±8.9 U·g(-1) in sedentary group vs. 773.3±62.6 U·g(-1) in trained group, P<0.05 and CS: 374.0±6.0 U·g(-1) in sedentary group vs. 534.2±20.5 U·g(-1) in trained group, P<0.01, respectively) whereas no changes were observed in soleus of IL6(-/-) mice. Moreover, in mixed gastrocnemius muscle of trained IL-6(-/-) mice enzyme activities tended to be lower (COX: 410.7±48.4 U·g(-1) for sedentary vs. 277.0±36.5 U·g(-1) for trained group and CS: 343.8±24.6 U·g(-1) for sedentary vs. 251.7±27.1 U·g(-1) for trained group). No changes in V'O2max were observed in WT and IL-6(-/-) mice after training. Concluding, moderate-velocity endurance training-induced increase in COX and CS activities in muscles of WT mice only which suggests that IL-6 regulates training-induced skeletal muscle responses to exercise.
Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland.
M
M
Wojewoda
Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland.
K
K
Kmiecik
Department of Physiology and Biochemistry, University School of Physical Education, Krakow, Poland.
J
J
Majerczak
University Paris-Sud, U-769 INSERM, Paris, France.
R
R
Ventura-Clapier
University Paris-Sud, U-769 INSERM, Paris, France.
D
D
Fortin
Nencki Institute of Experimental Biology, Laboratory of Cellular Metabolism, Krakow, Poland.
M
M
Onopiuk
Nencki Institute of Experimental Biology, Laboratory of Cellular Metabolism, Krakow, Poland.
J
J
Rog
Department of Cardiology, Medical University of Bialystok, Bialystok, Poland.
K
K
Kaminski
Jagiellonian Centre for Experimental Therapeutics, Laboratory of Experimental Pharmacology of Endothelium, Jagiellonian University, Krakow, Poland.
S
S
Chlopicki
Department of Physiology and Biochemistry, University School of Physical Education, Krakow, Poland.
J A
JA
Zoladz
D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism*; D015850:Interleukin-6 / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000201:enzymology; D010101:Oxygen Consumption / Q000502:physiology*; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000502:physiology*
D015850:Interleukin-6; C577686:interleukin-6, mouse; D045304:Cytochromes c; D002950:Citrate (si)-Synthase