Relationship between Targeting Efficacy of Liposomes and the Dosage of Targeting Antibody Using Surface Plasmon Resonance.
Surface plasmon resonance (SPR) was used in this research to investigate the targeting efficacy (i.e., the binding affinity) of antibody-modified liposomes. The results indicated that liposomes modified by targeting antibodies exhibited an increase in apparent binding affinity, a result attributed to the avidity effect. More specifically, the targeting effect improved as the surface density of the targeting antibody increased, an increase primarily attributed to the decrease of the dissociation rate. However, this trend stopped when the surface density reached a threshold of approximately 1.5 × 10(8) antibody/mm(2). This surface density was found to be quite consistent regardless of the liposome size and the type of targeting antibody. In addition, a traditional cell binding experiment was conducted to confirm the saturation point obtained from SPR.
Department of Bioengineering, Clemson University , Clemson, South Carolina 29634, United States.
Yun
Y
Xiang
Department of Bioengineering, Clemson University , Clemson, South Carolina 29634, United States.
Raisa
R
Kiseleva
Department of Bioengineering, Clemson University , Clemson, South Carolina 29634, United States.
Vladimir
V
Reukov
Ralph H. Johnson VA Medical Center , Charleston, South Carolina 29401, United States.
Jennifer
J
Mulligan
Ralph H. Johnson VA Medical Center , Charleston, South Carolina 29401, United States.
Carl
C
Atkinson
Ralph H. Johnson VA Medical Center , Charleston, South Carolina 29401, United States.
Rodney
R
Schlosser
Department of Bioengineering, Clemson University , Clemson, South Carolina 29634, United States.
Alexey
A
Vertegel
D000906:Antibodies / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D008081:Liposomes; D020349:Surface Plasmon Resonance
D000906:Antibodies; D008081:Liposomes