Alzheimer's disease is associated with disordered localization of ganglioside GM1 molecular species in the human dentate gyrus.
Alzheimer's disease (AD) is a progressive dementia associated with loss of memory and cognitive dysfunction. In a previous study, we demonstrated a decrease in b-series gangliosides along with a change in ganglioside molecular species in the hippocampal grey matter of patients with AD. The present study demonstrates the use of imaging mass spectrometry for analyzing the spatial arrangement of ganglioside GM1 (GM1) molecular species in the hippocampus. In AD patients, we found a decrease in the ratio of GM1(d20:1/C18:0) to GM1 d18:1/C18:0) in the outer molecular layer (ML) of the dentate gyrus. Because the outer ML is the region of main input into the hippocampus, our findings may have a direct relationship to the mechanism of dysfunction in AD.
Third Institute of New Drug Discovery, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan. Electronic address: Hirano.Wakako@otsuka.jp.
Wakako
W
Hirano-Sakamaki
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Eiji
E
Sugiyama
Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Sapporo 060-0812, Japan.
Takahiro
T
Hayasaka
Brain Bank Consultants, Meibergdreef 1105 AZ, Amsterdam, The Netherlands.
Rivka
R
Ravid
Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Mitsutoshi
M
Setou
Tokushima Institute, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan.
Takao
T
Taki
D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001692:Biological Transport; D018891:Dentate Gyrus / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged
D005677:G(M1) Ganglioside
Alzheimer’s disease; GM1; Ganglioside; Hippocampus; Imaging mass spectrometry; Postmortem brain