Using a Handheld Gene Gun for Genetic Transformation of Tetrahymena thermophila.
This chapter describes protocols for using a handheld gene gun to deliver transformation vectors for overexpression of genes or gene replacement in the macronucleus of Tetrahymena thermophila. The protocols provide helpful information for preparing Tetrahymena for biolistic bombardment, preparation of vector-coated microcarriers, and basic gene gun operating procedures.
Department of Biology, Technion - Israel Institute of Technology, Technion, Haifa, 3200003, Israel. mgotesman@tx.technion.ac.il.
Michael
M
Gotesman
Department of Biology, Miami Dade College, Miami, FL, USA.
Selwyn A
SA
Williams
D019470:Biolistics / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry; D005822:Genetic Vectors / Q000235:genetics; D006046:Gold / Q000737:chemistry; D010969:Plastics; D016808:Tetrahymena thermophila / Q000235:genetics*; D014170:Transformation, Genetic
D004337:Drug Carriers; D010969:Plastics; D006046:Gold; D004247:DNA
Biolistic bombardment; Gene gun; Gene replacement; Microcarriers; Overexpression; Tetrahymena thermophila