Precancerous esophageal epithelia are associated with significantly increased scattering coefficients.
The progression of epithelial precancers into cancer is accompanied by changes of tissue and cellular structures in the epithelium. Correlations between the structural changes and scattering coefficients of esophageal epithelia were investigated using quantitative phase images and the scattering-phase theorem. An ex vivo study of 14 patients demonstrated that the average scattering coefficient of precancerous epithelia was 37.8% higher than that of normal epithelia from the same patient. The scattering coefficients were highly correlated with morphological features including the cell density and the nuclear-to-cytoplasmic ratio. A high interpatient variability in scattering coefficients was observed and suggests identifying precancerous lesions based on the relative change in scattering coefficients.
Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.
Jing-Wei
JW
Su
Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.
Yang-Hsien
YH
Lin
Department of Dentistry, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Chun-Ping
CP
Chiang
Department of Surgery, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Jang-Ming
JM
Lee
Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan.
Chao-Mao
CM
Hsieh
Department of Pathology, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Min-Shu
MS
Hsieh
Department of Surgery, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Pei-Wen
PW
Yang
Department of Otolaryngology, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Chen-Ping
CP
Wang
Department of Internal Medicine, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Ping-Huei
PH
Tseng
Department of Internal Medicine, National Taiwan University Hospital, Taiwan ; National Taiwan University College of Medicine, Taiwan.
Yi-Chia
YC
Lee
Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taiwan ; Department of Electrical Engineering, National Taiwan University, Taiwan ; Molecular Imaging Center, National Taiwan University, Taiwan.
Kung-Bin
KB
Sung
(170.0180) Microscopy; (170.4580) Optical diagnostics for medicine; (170.6935) Tissue characterization; (290.7050) Turbid media