Absinthin attenuates LPS-induced ALI through MIP-1α-mediated inflammatory cell infiltration.
Acute lung injury (ALI) is characterized by severe lung inflammation, and anti-inflammatory treatment is proposed to be a pertinent therapeutic strategy for the disease. Absinthin is a triterpene, extracted from a Chinese herb, with anti-inflammatory properties. The aim of this study was to evaluate whether absinthin can attenuate ALI in a mouse model of lung injury. Mice were treated with various concentrations (20 mg/kg, 40 mg/kg, and 80mg/kg) of absinthin, and lipopolysaccharide (LPS) to induce ALI. We found that the administration of absinthin relieved LPS-induced acute lung injury, as suggested by reduced histological scores, wet-to-dry ratio, myeloperoxidase activity, and accumulation of inflammatory cells in lung bronchoalveolar lavage fluid. Moreover, we demonstrated that absinthin significantly enhanced the expression of matrix metalloproteinase-8 (MMP-8); this effect could inhibit the accumulation of inflammatory cells in lung tissues through a mechanism dependent on MMP-8-mediated inactivation of macrophage inflammatory protein-1α. Therefore, we propose that absinthin is a promising novel therapeutic candidate for the treatment of ALI.
a Department of Anesthesiology, Huadong Hospital Affiliated to Fudan University , Shanghai , China.
Nailiang
N
Guo
a Department of Anesthesiology, Huadong Hospital Affiliated to Fudan University , Shanghai , China.
Yinghua
Y
Xu
a Department of Anesthesiology, Huadong Hospital Affiliated to Fudan University , Shanghai , China.
Zhongqiang
Z
Cao
D055371:Acute Lung Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D054405:Chemokine CCL3 / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D020784:Matrix Metalloproteinase 8 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D045789:Sesquiterpenes, Guaiane / Q000494:pharmacology*
C496534:Ccl3 protein, mouse; D054405:Chemokine CCL3; D016207:Cytokines; D008070:Lipopolysaccharides; D045789:Sesquiterpenes, Guaiane; C499475:absinthin; D009195:Peroxidase; C576328:MMP8 protein, mouse; D020784:Matrix Metalloproteinase 8
absinthin, MIP-1α, acute lung injury, inflammation, MMPs