In vitro induction and proteomics characterisation of a uranyl-protein interaction network in bovine serum.
Uranyl ions (UO2(2+)) were shown to interact with a number of foetal serum proteins, leading to the formation of a complex that could be isolated by ultracentrifugation. The molecular weight of the complex was estimated based on size-exclusion chromatography as 650 000 Da. Online ICP AES detection indicated that UO2(2+) in the complex co-eluted with minor amounts of calcium and phosphorous, but not with magnesium. A 1D gel electrophoresis of the U-complex produced more than 10 bands of similar intensity compared with only 2-3 intense bands corresponding to the main serum proteins in the control serum, indicative of the specific interaction of UO2(2+) with minor proteins. A proteomics approach allowed for the identification of 74 proteins in the complex. Analysis of the protein-protein interaction network in the UO2(2+) complex identified 32 proteins responsible for protein-protein complex formation and 34 with demonstrated ion-binding function, suggesting that UO2(2+) stimulates the formation of protein functional networks rather than using a particular molecule as its target.
CNRS/UPPA, LCABIE, UMR5254, Hélioparc, 2, av. Pr. Angot, F-64053 Pau, France. lukasz.szyrwiel@umed.wroc.pl.
Łukasz
Ł
Szyrwiel
Viktoryia
V
Liauchuk
Laurent
L
Chavatte
Ryszard
R
Lobinski
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D017974:Uranium Compounds / Q000737:chemistry* / Q000378:metabolism*
D001798:Blood Proteins; D017974:Uranium Compounds; C012597:uranium dioxide