The rs1803274 polymorphism of the BCHE gene is associated with an increased risk of coronary in-stent restenosis.
BACKGROUND
We sought to identify gene polymorphisms that confer susceptibility to in-stent restenosis after coronary artery bare-metal stenting in a Central European population.


METHODS
160 controls without post-percutaneous coronary intervention in-stent restenosis were matched for age, sex, vessel diameter, and diabetes to 160 consecutive cases involving in-stent restenosis of the target lesion within 12 months. Using real time polymerase chain reaction and melting-curve analysis, we detected 13 single-nucleotide polymorphisms in 11 candidate genes - rs1803274 (BCHE gene), rs529038 (ROS1), rs1050450 (GPX1), rs1800849 (UCP3), rs17216473 (ALOX5AP), rs7412, rs429358 (ApoE), rs2228570 (VDR), rs7041, rs4588 (GC), rs1799986 (LRP1) and rs2228671 (LDLR). Multivariable logistic regression was used to test for associations.


RESULTS
The rs1803274 polymorphism of BCHE was significantly associated with in-stent restenosis (OR 1.934; 95 % CI: 1.181-3.166; p = 0.009). No association was found with the other studied SNPs.


CONCLUSIONS
The A allele of rs1803274 represents a risk factor for in-stent restenosis in Central European patients after percutaneous coronary intervention with bare-metal stent implantation.
Department of Clinical Studies, Medical Faculty, University of Ostrava, Syllabova 19, Ostrava, Zabreh, 703 00, Czech Republic. leos.pleva@volny.cz.|Department of Cardiovascular Diseases, University Hospital Ostrava, Ostrava, Czech Republic. leos.pleva@volny.cz.
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Pleva
Department of Biomedical Sciencies, Medical Faculty, University of Ostrava, Ostrava, Czech Republic. petra.kovarova@fno.cz.|Blood Center, University Hospital of Ostrava, Ostrava, Czech Republic. petra.kovarova@fno.cz.
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Kovarova
Department of Biomedical Sciencies, Medical Faculty, University of Ostrava, Ostrava, Czech Republic. lucie.faldynova@seznam.cz.|Department of Medical Genetics, University Hospital Ostrava, Ostrava, Czech Republic. lucie.faldynova@seznam.cz.
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Faldynova
Department of Biomedical Sciencies, Medical Faculty, University of Ostrava, Ostrava, Czech Republic. pavlina.plevova@volny.cz.|Department of Medical Genetics, University Hospital Ostrava, Ostrava, Czech Republic. pavlina.plevova@volny.cz.
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Plevova
Department of Biomedical Sciencies, Medical Faculty, University of Ostrava, Ostrava, Czech Republic. HilscherovaS@seznam.cz.|Department of Medical Genetics, University Hospital Ostrava, Ostrava, Czech Republic. HilscherovaS@seznam.cz.
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Hilscherova
Department of Medical Biophysics, Palacky University, Olomouc, Czech Republic. jana@tunw.upol.cz.
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Zapletalova
Department of Biomedical Sciencies, Medical Faculty, University of Ostrava, Ostrava, Czech Republic. kusnierova.pavlina@seznam.cz.|Department of Laboratory Medicine, University Hospital Ostrava, Ostrava, Czech Republic. kusnierova.pavlina@seznam.cz.
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Kusnierova
Department of Cardiovascular Diseases, University Hospital Ostrava, Ostrava, Czech Republic. paja.kukla@gmail.com.
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Kukla
D000368:Aged; D000483:Alleles; D002091:Butyrylcholinesterase / Q000235:genetics*; D023903:Coronary Restenosis / Q000235:genetics* / Q000601:surgery*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D062645:Percutaneous Coronary Intervention / Q000295:instrumentation; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D015607:Stents
D002091:Butyrylcholinesterase