Comparison of innate immunity mediators in peritoneal fluid and spleen between young and aged rats.
BACKGROUND
Aging of the innate immune system can result in a wide array of decreasing biological function.


OBJECTIVE
We investigated differences between young and old mice in the expression of the pattern-recognition receptors (PRRs).


METHODS
mRNA levels for PRRs were quantified and compared in peritoneal fluid and spleens from old (36 months old) and young (1 month old) Wistar white rats (n = 8/group) using real-time reverse transcription-polymerase chain reaction.


RESULTS
In old rats, TLR-5 and -7 were decreased in peritoneal fluid, whereas TLR 4/6/9 and Syk were increased in the spleen (p < 0.05). In young rats, TLR 2/4, dectin-1, and Trem-1 were increased in peritoneal fluid and decreased in the spleen (p < 0.05), but TLR 1/3/7/9/10 and Syk were vice versa (p < 0.05).


CONCLUSIONS
Several parameters related to innate immunity may change with aging.


CONCLUSIONS
Different expressions of mRNA for several PRRs are suggesting changes in innate immune responses in association with aging.
Department of Obstetrics and Gynecology, School of Medicine, Kyung Hee University, Seoul, Korea.
Young Joon
YJ
Choi
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Korea.
Seung Geun
SG
Yeo
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Korea.
Young Il
YI
Kim
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Korea.
Jin-Woo
JW
Lee
Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Korea.
Sang Hoon
SH
Kim
Department of Obstetrics and Gynecology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, 93 Gi-dong, Paldal-ku, Suwon, 442-723, Korea. park.dongchoon@gmail.com.
Dong Choon
DC
Park
D000375:Aging / Q000276:immunology; D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology*; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000235:genetics / Q000502:physiology; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D051192:Receptors, Pattern Recognition / Q000502:physiology; D013154:Spleen / Q000276:immunology*; D051193:Toll-Like Receptors / Q000235:genetics / Q000502:physiology; D000073871:Triggering Receptor Expressed on Myeloid Cells-1 / Q000235:genetics / Q000502:physiology; D000099181:Dectin-1
D037181:Lectins, C-Type; D012333:RNA, Messenger; D051192:Receptors, Pattern Recognition; D051193:Toll-Like Receptors; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D000099181:Dectin-1
Aging; Innate immunity; Pattern recognition receptors; Peritoneal fluid; Spleen