Evaluation of Pseudoenantiomeric Mixed Carbonates as Efficient Fluorogenic Probes for Enantioselectivity Screening.
We report mixed carbonates as enzyme substrates and demonstrate their application as fluorogenic probes for lipase and esterase enantiopreference screening. By the application of pseudoenantiomers with distinct fluorescence behaviors, it is possible to evaluate the activity and enantiopreference of hydrolytic enzymes. In order to validate our method, enantioselectivities calculated from fluorometric measurements were compared with the results obtained from larger-scale kinetic resolution.
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Anna
A
Zadlo
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Dominik
D
Koszelewski
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Filip
F
Borys
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland. r.ostaszewski@icho.edu.pl.
Ryszard
R
Ostaszewski
D002254:Carbonates / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D002254:Carbonates; D005456:Fluorescent Dyes; D004950:Esterases; D008049:Lipase
enantioselectivity; fluorescent probes; fluorogenic assay; high-throughput screening; pseudoenantiomers