Significance of detecting IgH and TCRγ gene rearrangements in patients with hemopoietic maligancies by real-time quantitative PCR.
The aim of our study was to investigate the association of IgH and TCRγ gene rearrangements in hematological malignancies with the disease and clinical application. IgH and TCRγ gene rearrangements were determined in 69 paraffin and bone marrow specimens with SYBR Green I fluorescent dye and RQ-PCR method, including 21 paraffin-embedded tissues of the onset cases and 48 bone marrow samples, representing 15 ALL and 25 AML cases. After chemotherapy, 8 cases were NHL; the 10 cases of the negative control group were healthy people. Among the ALL cases, the IgH rearrangement occurred in 80.0%, the TCRγ rearrangement in 46.7%, and both gene rearrangements in 46.7%. Among the AML cases, the IgH rearrangement occurred in 72.0%, the TCRγ rearrangement in 68.0%, and both gene rearrangements in 60.0%. In the lymphoma cases, the IgH rearrangement occurred in 93.1%, the TCRγ rearrangement in 51.7%, and both gene rearrangements in 44.8%. In the negative control group, the 10 cases were all negative. There was the phenomenon of "sequence-non-fidelity" in the hematologic malignancies; the detection rate of both genes was much higher than that of the single gene. The application of the RQ-PCR method in the detection of IgH and TCRγ gene rearrangements in hematologic malignancies has important clinical significance in MRD monitoring.
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China JinYaodoc@163.com.
J
J
Yao
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
R H
RH
Wang
Department of Orthopedic, The First Afiliated Hospital of Kunming Medical University, Kunming, China.
B
B
Li
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Y
Y
Wang
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
P
P
Zhu
Department of Hematology Onset Molecular Biology Laboratory, The Second People's Hospital of Yunnan Province, Kunming, China.
X J
XJ
Li
Department of Kunming Medical University, Kunming, China.
X Q
XQ
Ma
Department of Kunming Medical University, Kunming, China.
M J
MJ
Ma
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
L F
LF
Yin
D000328:Adult; D017931:DNA Primers / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D019675:Genes, T-Cell Receptor gamma / Q000235:genetics*; D019337:Hematologic Neoplasms / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains