Transcriptional analysis of the dachsous gene uncovers novel isoforms expressed during development in Drosophila.
The Drosophila cadherin-related protein Dachsous (Ds) plays a prominent role in planar cell polarity (PCP) and growth. The regulation of these two processes is based on the interaction between Ds and Fat proteins, generating an intracellular response required for tissue polarization and modulation of Hippo pathway activity. Here we have performed a comprehensive molecular study of the ds gene during larval development that has shown an unexpected complexity in its transcriptional regulation and revealed the expression of hitherto unsuspected transcripts. Also, knockdown of several isoforms provides new evidence on the importance of the cytoplasmic domain in the mechanism of action of Ds during development.
Centro de Biología Molecular "Severo Ochoa", Nicolas Cabrera, 1, Campus Cantoblanco, 28049 Madrid, Spain.
Eva
E
Revilla-Yates
Centro de Biología Molecular "Severo Ochoa", Nicolas Cabrera, 1, Campus Cantoblanco, 28049 Madrid, Spain.
Laura
L
Varas
Departamento de Biotecnología, Universidad Francisco de Vitoria, 28223 Pozuelo de Alarcón, Madrid, Spain.
Javier
J
Sierra
Centro de Biología Molecular "Severo Ochoa", Nicolas Cabrera, 1, Campus Cantoblanco, 28049 Madrid, Spain. Electronic address: isabel.rodriguez@mineco.es.
Isabel
I
Rodriguez
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D015820:Cadherins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016764:Cell Polarity; D003593:Cytoplasm / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D005091:Exons / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000254:growth & development; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D014158:Transcription, Genetic
D015820:Cadherins; D029721:Drosophila Proteins; D020033:Protein Isoforms; C094449:ds protein, Drosophila
Dachsous transcript; Drosophila; DsIntra isoform; Growth; Planar cell polarity