Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment.
A continuous process based on the combination of ultrasounds and/or microwaves pretreatments followed by enzymatic hydrolysis and simultaneous extraction (EHSE) has been proposed to recover genipin from Eucommia ulmoides bark. At first, in the pretreatment step, the mixture of 1.0 g dried bark powder and 10 mL deionized water were irradiated by microwave under 500 W for 10 min. Then, in hydrolysis step, the optimal conditions were as follows: 0.5 mg/mL of cellulase concentration, 4.0 pH of enzyme solution, 24 h of incubation time and 40 °C of incubation temperature. After incubation, 10 mL ethanol was added to extract genipin for 30 min by ultrasound. After EHSE treatment, the yield of genipin could reach 1.71 μmol/g. Moreover, scanning electron micrographs illustrated that severe structural disruption of plant was obtained by EHSE. The results indicated that the EHSE method provided a good alternative for the preparation of genipin from Eucommia ulmoides bark as well as other herbs.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. lilili001@126.com.|State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. lilili001@126.com.
Lili
L
Li
College of Animal Science and Technology, Hebei North University, Zhangjiakou 075000, China. yguohb@163.com.
Yupin
Y
Guo
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. zhaolianfei2013@126.com.|State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. zhaolianfei2013@126.com.
Lianfei
L
Zhao
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. zygorl@126.com.|State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. zygorl@126.com.
Yuangang
Y
Zu
School of Forestry, Northeast Forestry University, Harbin 150040, China. hygnefu@126.com.
Huiyan
H
Gu
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. yanglei@nefu.edu.cn.|State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China. yanglei@nefu.edu.cn.
Lei
L
Yang
D002480:Cellulase / Q000737:chemistry*; D004307:Dose-Response Relationship, Radiation; D000431:Ethanol / Q000737:chemistry*; D031283:Eucommiaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D039823:Iridoids / Q000302:isolation & purification*; D059627:Liquid Phase Microextraction / Q000379:methods*; D008872:Microwaves; D024301:Plant Bark / Q000737:chemistry*; D013010:Sonication; D013696:Temperature
D039823:Iridoids; D000431:Ethanol; C007834:genipin; D002480:Cellulase
Eucommia ulmoides bark; enzymatic hydrolysis and simultaneous extraction; genipin; ultrasound/microwave irradiation pretreatment