Fourier transform infrared spectroscopy (FTIR) characterization of the interaction of anti-cancer photosensitizers with dendrimers.
The systemic or local administration of a photosensitizer for photodynamic therapy is highly limited by poor selectivity, rapid deactivation and long-lasting skin toxicity due to unfavorable biodistribution. Drug delivery systems based on nanocarriers may help specific and effective delivery of photosensitizers. In the present paper, the interaction of two photosensitizers, methylene blue and rose bengal, with phosphorous cationic and anionic dendrimers as potential nanocarriers, has been characterized. A novel method is presented based on the analysis of the infrared spectra of mixtures of photosensitizer and dendrimer. The capacity of dendrimers to bind the photosensitizers has been evaluated by obtaining the corresponding binding curves. It is shown that methylene blue interacts with both cationic and anionic dendrimers, whereas rose bengal only binds to the cationic ones. Dendrimers are shown to be potential nanocarriers for a specific delivery of both photosensitizers.
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland.
Monika
M
Dabrzalska
ALBA Synchrotron, BP-1413 km 3,3, 08290, Cerdanyola del Vallès, Catalonia, Spain.
Nuria
N
Benseny-Cases
Biophysics Unit and Center of Studies in Biophysics, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain.
Ramon
R
Barnadas-Rodríguez
PRES Sorbonne Paris Cité, CNRS UMR 860, Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, Université Paris Descartes, 45 rue des Saints Pères, 75006, Paris, France.
Serge
S
Mignani
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363, Lodz, Poland.
Maria
M
Zablocka
Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077, Toulouse, France.|Université de Toulouse, UPS, INPT, 31077, Toulouse Cedex 4, France.
Jean-Pierre
JP
Majoral
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland.
Maria
M
Bryszewska
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland.|Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, 01069, Dresden, Germany.
Barbara
B
Klajnert-Maculewicz
Biophysics Unit and Center of Studies in Biophysics, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain. josep.cladera@uab.cat.
Josep
J
Cladera
D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000295:instrumentation; D006801:Humans; D008751:Methylene Blue / Q000008:administration & dosage / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry*; D012395:Rose Bengal / Q000008:administration & dosage / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D050091:Dendrimers; D004337:Drug Carriers; D017319:Photosensitizing Agents; D012395:Rose Bengal; D008751:Methylene Blue
Dendrimer-photosensitizer interactions; Infrared spectroscopy; Methylene blue; Phosphorus dendrimer; Rose bengal