title: Global Profiling of Carbohydrate Active Enzymes in Human Gut Microbiome.
abstract: BACKGROUND
  Carbohydrate Active enzyme (CAZyme) families, encoded by human gut microflora, play a crucial role in breakdown of complex dietary carbohydrates into components that can be absorbed by our intestinal epithelium. Since nutritional wellbeing of an individual is dependent on the nutrient harvesting capability of the gut microbiome, it is important to understand how CAZyme repertoire in the gut is influenced by factors like age, geography and food habits.
  
  
  RESULTS
  This study reports a comprehensive in-silico analysis of CAZyme profiles in the gut microbiomes of 448 individuals belonging to different geographies, using similarity searches of the corresponding gut metagenomic contigs against the carbohydrate active enzymes database. The study identifies a core group of 89 CAZyme families that are present across 85% of the gut microbiomes. The study detects several geography/age-specific trends in gut CAZyme repertoires of the individuals. Notably, a group of CAZymes having a positive correlation with BMI has been identified. Further this group of BMI-associated CAZymes is observed to be specifically abundant in the Firmicutes phyla. One of the major findings from this study is identification of three distinct groups of individuals, referred to as 'CAZotypes', having similar CAZyme profiles. Distinct taxonomic drivers for these CAZotypes as well as the probable dietary basis for such trends have also been elucidated. The results of this study provide a global view of CAZyme profiles across individuals of various geographies and age-groups. These results reiterate the need of a more precise understanding of the role of carbohydrate active enzymes in human nutrition.
authors.affiliation: Teach for India, A 903, Tain Square, Fatima Nagar, Pune, Maharashtra, India.
authors.forename: Tanudeep
authors.initials: T
authors.lastname: Bhattacharya
authors.affiliation: TCS Innovation Labs, Tata Consultancy Services Ltd.,54-B, Hadapsar Industrial Estate, Pune, Maharashtra, India.
authors.forename: Tarini Shankar
authors.initials: TS
authors.lastname: Ghosh
authors.affiliation: TCS Innovation Labs, Tata Consultancy Services Ltd.,54-B, Hadapsar Industrial Estate, Pune, Maharashtra, India.
authors.forename: Sharmila S
authors.initials: SS
authors.lastname: Mande
mesh_terms: D000328:Adult; D000367:Age Factors; D050260:Carbohydrate Metabolism / Q000235:genetics*; D002648:Child; D004040:Dietary Carbohydrates / Q000378:metabolism; D004950:Esterases / Q000235:genetics*; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics*; D006801:Humans; D008190:Lyases / Q000235:genetics*; D064307:Microbiota / Q000235:genetics*
chemical_list: D004040:Dietary Carbohydrates; D004950:Esterases; D006026:Glycoside Hydrolases; D008190:Lyases