title: Interaction of partially denatured insulin with a DSPC floating lipid bilayer.
abstract: The carefully controlled permeability of cellular membranes to biological molecules is key to life. In degenerative diseases associated with protein misfolding and aggregation, protein molecules or their aggregates are believed to permeate these barriers and threaten membrane integrity. We used neutron reflectivity to study the interaction of insulin, a model amyloidogenic protein, with a DSPC floating lipid bilayer. Structural changes consistent with protein partitioning to the membrane interior and adsorption to a gel phase model lipid bilayer were observed under conditions where the native fold of the protein is significantly destabilised. We propose that the perturbation of the membrane by misfolded proteins involves long term occupation of the membrane by these proteins, rather than transient perforation events.
authors.affiliation: University Grenoble Alpes, IBS, F-38044 Grenoble, France. andrewdennison@live.com.
authors.forename: A J C
authors.initials: AJ
authors.lastname: Dennison
authors.forename: R A L
authors.initials: RA
authors.lastname: Jones
authors.forename: R A
authors.initials: RA
authors.lastname: Staniforth
authors.forename: A J
authors.initials: AJ
authors.lastname: Parnell
mesh_terms: D000818:Animals; D002417:Cattle; D002463:Cell Membrane Permeability; D007328:Insulin / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D066329:Protein Aggregates; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D007328:Insulin; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D066329:Protein Aggregates; C010942:1,2-distearoyllecithin