title: Streptomyces hyaluromycini sp. nov., isolated from a tunicate (Molgula manhattensis).
abstract: A novel Gram-stain-positive actinomycete, designated MB-PO13(T), was isolated from a tunicate (Molgula manhattensis) collected in Tokyo Bay, Japan, and its taxonomic position was studied by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MB-PO13(T) was closely related to Streptomyces graminisoli JR-12(T) (99.72% 16S rRNA gene sequence similarity) and Streptomyces shenzhenensis 172115(T) (99.23%). The strain contained LL-diaminopimelic acid in the whole-cell hydrolysate. The predominant menaquinones were MK-9(H8) and MK-9(H6) and the major fatty acids were anteiso-C15:0, iso-C16:0, iso-C14:0 and C16:0. These data supported the affiliation of the novel strain to the genus Streptomyces. Meanwhile, results of DNA-DNA hybridization and physiological and biochemical tests indicated that strain MB-PO13(T) was distinguished from known Streptomyces type strains. Therefore, strain MB-PO13(T) represents a novel species of the genus Streptomyces for which the name Streptomyces hyaluromycini sp. nov. is proposed; the type strain is MB-PO13(T) (=NBRC 110483(T) =DSM 100105(T)).
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan.
authors.forename: Enjuro
authors.initials: E
authors.lastname: Harunari
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Kisarazu, Chiba, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Kisarazu, Chiba, Japan.
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shibata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Kisarazu, Chiba, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Tamura
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Kisarazu, Chiba, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.affiliation: Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Imada
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014561:Urochordata / Q000382:microbiology*; D024482:Vitamin K 2 / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid