title: [Expression of Hydrodynamic Injection-mediated PD-L1 in Myeloablative Conditioning Mouse Model].
abstract: OBJECTIVE
  To establish the mouse model for the expression of PD-L1 by hydrodynamic injection and to study the effects of myeloablative conditioning on hydrodynamic injection-mediated PD-L1 expression.
  
  
  METHODS
  Plasmid amplification, hydrodynamic injection, collagenase perfusion, real time PCR, ELISA and flow cytometry were applied to test the expression and function of PD-L1. Also, animal models were set up to test the effects of chemical or radiactive myeloablative conditioning on hydrodynamic injection-mediated PD-L1 expression.
  
  
  RESULTS
  The expression of PD-L1 mRNA and protein could be detected as early as 8 h after hyrodynamic injection and reached peak expression by 24 h, and returned to baseline level by 7 d after injection. Serum PD-L1 level reached to 100 µg/ml as early as 24 h after injection and plateaued at 7 d after injection. Serum PD-L1 persisted for 3 weeks and declined to baseline after 1 month of hydrodynamic injection. The PD-L1 function induced by hydrodynamic injection was consistent with literature reports. At each time point, the PD-L1 expression was not different significantly between the myeloablative conditioning group and control group; the mice transfected with PD-L1 showed a higher survival rate than that in control group.
  
  
  CONCLUSIONS
  Myeloablative conditioning does not affect hydrodynamic injection-mediated PD-L1 expression, indicating that the PD-L1 can be used in HSCT mouse model.
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Li
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China. E-mail: nainli@aliyun.com.
authors.forename: Nai-Nong
authors.initials: NN
authors.lastname: Li
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Feng-E
authors.initials: FE
authors.lastname: Yang
authors.affiliation: Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou 350001, Fujian Province, China.
authors.forename: Yuan-Zhong
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D060890:B7-H1 Antigen / Q000494:pharmacology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D057446:Hydrodynamics; D007267:Injections; D051379:Mice; D019653:Myeloablative Agonists / Q000494:pharmacology*; D012333:RNA, Messenger; D014162:Transfection; D019172:Transplantation Conditioning
chemical_list: D060890:B7-H1 Antigen; D019653:Myeloablative Agonists; D012333:RNA, Messenger