title: [Phylogenetic Analyses and Characterization of the VP7 Genes of Serotype G9 Rotaviruses Circulating in Nanjing (China), 2012-2013].
abstract: Group-A rotaviruses are recognized as the most common cause of acute diarrhea. Phylogenetic analyses of the VP7 genes of rotaviruses circulating in Nanjing (China) could aid in the development of rotavirus vaccines. A total of 908 stool specimens were collected from patients suffering from acute diarrhea in Nanjing between October 2012 and December 2013, and were tested further for rotaviruses. Fifty rotavirus isolates selected randomly were typed by reverse transcription-polymerase chain reaction using serotype-specific primers for G genotyping. VP7 genes of 19 G9 strains were sequenced for further genetic characterization. Among the 908 stool specimens examined during the surveillance period, 103 (11.34%) were rotavirus-positive. G9 was the most predominant genotype (78.0%), followed by G2, G1 and G3. Sequence and phylogenetic analyses of the VP7 genes of serotype G9 rotaviruses revealed these strains to comprise two lineages (G9-VI, G9-III) and to be dominated by the G9-VI lineage (which belonged to a unique subcluster of Japanese and Chinese G9 strains). Amino-acid sequences of the four antigenic regions (A, B, C or F) were variant among a portion of strains, which may have contributed to the prevalence of G9 rotaviruses in this area.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Limin
authors.initials: L
authors.lastname: Shi
authors.forename: Mengkai
authors.initials: M
authors.lastname: Qiao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Yong
authors.forename: Xuefei
authors.initials: X
authors.lastname: Du
authors.forename: Baofu
authors.initials: B
authors.lastname: Guo
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.forename: Ziqian
authors.initials: Z
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002681:China; D019143:Evolution, Molecular; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D065288:Serogroup
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus