title: Identification and Application of Neutralizing Epitopes of Human Adenovirus Type 55 Hexon Protein.
abstract: Human adenovirus type 55 (HAdV55) is a newly identified re-emergent acute respiratory disease (ARD) pathogen with a proposed recombination of hexon gene between HAdV11 and HAdV14 strains. The identification of the neutralizing epitopes is important for the surveillance and vaccine development against HAdV55 infection. In this study, four type-specific epitope peptides of HAdV55 hexon protein, A55R1 (residues 138 to 152), A55R2 (residues 179 to 187), A55R4 (residues 247 to 259) and A55R7 (residues 429 to 443), were predicted by multiple sequence alignment and homology modeling methods, and then confirmed with synthetic peptides by enzyme-linked immunosorbent assay (ELISA) and neutralization tests (NT). Finally, the A55R2 was incorporated into human adenoviruses 3 (HAdV3) and a chimeric adenovirus rAd3A55R2 was successfully obtained. The chimeric rAd3A55R2 could induce neutralizing antibodies against both HAdV3 and HAdV55. This current study will contribute to the development of novel adenovirus vaccine candidate and adenovirus structural analysis.
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510230, China. tianxingui7902@aliyun.com.
authors.forename: Xingui
authors.initials: X
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510230, China. mat008@163.com.|Dongguan Institute of Pediatrics, Dongguan Children's Hospital, Dongguan 523325, China. mat008@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Dongguan Institute of Pediatrics, Dongguan Children's Hospital, Dongguan 523325, China. jiangzaixue160368@126.com.
authors.forename: Zaixue
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. jfhuang.dg@gmail.com.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: Dongguan Institute of Pediatrics, Dongguan Children's Hospital, Dongguan 523325, China. qianliu_ln@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Dongguan Institute of Pediatrics, Dongguan Children's Hospital, Dongguan 523325, China. lxm020@126.com.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Lu
authors.affiliation: Dongguan Institute of Pediatrics, Dongguan Children's Hospital, Dongguan 523325, China. luoqingxian@163.com.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510230, China. zhourong@gird.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000517:prevention & control; D000260:Adenoviruses, Human / Q000276:immunology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000276:immunology*; D055808:Drug Discovery; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005260:Female; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; C018315:hexon capsid protein, Adenovirus
keywords: adenovirus type 55; bivalent vaccine; neutralizing epitope