title: Reciprocal expression of p-AMPKa and p-S6 is strongly associated with the prognosis of gastric cancer.
abstract: Activation of AMP-activated protein kinase (AMPK) suppressed mammalian target of rapamycin (mTOR) pathway, resulting in impaired cancer cell proliferation. Two cohorts (50 and 1072 cases) of patients with resected gastric adenocarcinoma were enrolled in the study. Immunohistochemical staining for p-AMPKa, p-ACC, p-mTOR, p-S6, and p-4EBP1 was performed on the 50-patient cohort. Tissue microarray blocks containing samples from 1072 patients of Chinese ethnicity were used for the immunohistochemical detection of p-AMPKa and p-S6 levels. p-AMPK and p-ACC were frequently inactivated in both cohorts of gastric cancer samples, while p-mTOR, p-S6, and p-4EBP1 were frequently activated in the small cohort of gastric cancer. However, only levels of p-AMPKa and p-S6 were associated with the overall survival of gastric cancer patients. In the larger 1072-patient cohort, downregulation of p-AMPKa and upregulation of p-S6 were associated with tumor progression and were independent predictors of survival after resection of primary gastric cancer. Therefore, reciprocal expression of p-AMPKa and p-S6 may be promising prognostic biomarkers in patients with gastric cancer.
authors.affiliation: Department of Oncology, Changzheng Hospital, Shanghai, 200070, China.|Department of Oncology, General Hospital of Shenyang Military Region, Shenyang, 110016, Jilin Province, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Oncology, The 371st Central Hospital, Xinxiang City, 453000, Henan Province, China.
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Internal Medicine, Emergency Center, East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
authors.forename: Meiyan
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Oncology, Changzheng Hospital, Shanghai, 200070, China. jiejunw@csco.org.cn.|Department of Medical Oncology, Shanghai East Hospital, Shanghai, 200120, China. jiejunw@csco.org.cn.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Oncology, Changzheng Hospital, Shanghai, 200070, China. qiaoshanqian@aliyun.com.|Department of Medical Oncology, Shanghai East Hospital, Shanghai, 200120, China. qiaoshanqian@aliyun.com.
authors.forename: Guanzhen
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Oncology, Fuzhou General Hospital, Fuzhou, 350025, Fujian Province, China. fangwenzheng@hotmail.com.
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Fang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis / Q000235:genetics; D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000235:genetics; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D018797:Cell Cycle Proteins; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D010766:Phosphorylation; D011379:Prognosis; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C087000:EIF4EBP1 protein, human; D009363:Neoplasm Proteins; D010750:Phosphoproteins; C546842:MTOR protein, human; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; C000588423:ACACA protein, human; D000103:Acetyl-CoA Carboxylase
keywords: Gastric carcinoma; Immunohistochemistry; Prognosis; p-4EBP1; p-ACC; p-AMPKa; p-S6; p-mTOR