title: Distribution of CD163-positive cell and MHC class II-positive cell in the normal equine uveal tract.
abstract: Antigen-presenting cells (APCs) in the uveal tract participate in ocular immunity including immune homeostasis and the pathogenesis of uveitis. In horses, although uveitis is the most common ocular disorder, little is known about ocular immunity, such as the distribution of APCs. In this study, we investigated the distribution of CD163-positive and MHC II-positive cells in the normal equine uveal tract using an immunofluorescence technique. Eleven eyes from 10 Thoroughbred horses aged 1 to 24 years old were used. Indirect immunofluorescence was performed using the primary antibodies CD163, MHC class II (MHC II) and CD20. To demonstrate the site of their greatest distribution, positive cells were manually counted in 3 different parts of the uveal tract (ciliary body, iris and choroid), and their average number was assessed by statistical analysis. The distribution of pleomorphic CD163- and MHC II-expressed cells was detected throughout the equine uveal tract, but no CD20-expressed cells were detected. The statistical analysis demonstrated the distribution of CD163- and MHC II-positive cells focusing on the ciliary body. These results demonstrated that the ciliary body is the largest site of their distribution in the normal equine uveal tract, and the ciliary body is considered to play important roles in uveal and/or ocular immune homeostasis. The data provided in this study will help further understanding of equine ocular immunity in the normal state and might be beneficial for understanding of mechanisms of ocular disorders, such as equine uveitis.
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Sano
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsuda
authors.forename: Minoru
authors.initials: M
authors.lastname: Okamoto
authors.forename: Kazushige
authors.initials: K
authors.lastname: Takehana
authors.forename: Kazuko
authors.initials: K
authors.lastname: Hirayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Taniyama
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018951:Antigens, CD20 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D002452:Cell Count; D002924:Ciliary Body / Q000166:cytology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005802:Genes, MHC Class II; D006736:Horses; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D008297:Male; D011956:Receptors, Cell Surface / Q000032:analysis*; D012016:Reference Values; D014602:Uvea / Q000166:cytology*; D014605:Uveitis / Q000276:immunology / Q000662:veterinary; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D018951:Antigens, CD20; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface