title: Quantitative nature of overexpression experiments.
abstract: Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using large-scale biological data sets, we need to recognize the quantitative nature of overexpression experiments. Here I discuss the quantitative features of two different types of overexpression experiment: absolute and relative. I also introduce the four primary mechanisms involved in growth defects caused by protein overexpression: resource overload, stoichiometric imbalance, promiscuous interactions, and pathway modulation associated with the degree of overexpression.
authors.affiliation: Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700-8530, Japan hisaom@cc.okayama-u.ac.jp.
authors.forename: Hisao
authors.initials: H
authors.lastname: Moriya
mesh_terms: D005069:Evaluation Studies as Topic; D018628:Gene Dosage; D015870:Gene Expression; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D060066:Protein Interaction Maps; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins