title: Transcriptome analysis and characterisation of gill-expressed carbonic anhydrase and other key osmoregulatory genes in freshwater crayfish Cherax quadricarinatus.
abstract: The pH and salinity balance mechanisms of crayfish are controlled by a set of transport-related genes. We identified a set of the genes from the gill transcriptome from a freshwater crayfish Cherax quadricarinatus using the Illumina NGS-sequencing technology. We identified and characterized carbonic anhydrase (CA) genes and some other key genes involved in systematic acid-base balance and osmotic/ionic regulation. We also examined expression patterns of some of these genes across different sublethal pH levels [1]. A total of 72,382,710 paired-end Illumina reads were assembled into 36,128 contigs with an average length of 800 bp. About 37% of the contigs received significant BLAST hits and 22% were assigned gene ontology terms. These data will assist in further physiological-genomic studies in crayfish.
authors.affiliation: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
authors.forename: Muhammad Yousuf
authors.initials: MY
authors.lastname: Ali
authors.affiliation: School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
authors.forename: Ana
authors.initials: A
authors.lastname: Pavasovic
authors.affiliation: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Mather
authors.affiliation: School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, QLD 4001, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Prentis
keywords: Crayfish; Redclaw; Salinity; Transport-gene; pH balance