title: Body distribution of SiO₂-Fe₃O₄ core-shell nanoparticles after intravenous injection and intratracheal instillation.
abstract: Nano-silicon dioxide (SiO2) is used nowadays in several biomedical applications such as drug delivery and cancer therapy, and is produced on an industrial scale as additive to paints and coatings, cosmetics and food. Data regarding the long-term biokinetics of SiO2 engineered nanoparticles (ENPs) is lacking. In this study, the whole-body biodistribution of SiO2 core-shell ENPs containing a paramagnetic core of Fe3O4 was investigated after a single exposure via intravenous injection or intratracheal instillation in mice. The distribution and accumulation in different organs was evaluated for a period of 84 days using several techniques, including magnetic resonance imaging, inductively coupled plasma mass spectrometry, X-ray fluorescence and X-ray absorption near edge structure spectroscopy. We demonstrated that intravenously administered SiO2 ENPs mainly accumulate in the liver, and are retained in this tissue for over 84 days. After intratracheal instillation, an almost complete particle clearance from the lung was seen after 84 days with distribution to spleen and kidney. Furthermore, we have strong evidence that the ENPs retain their original core-shell structure during the whole observation period. This work gives an insight into the whole-body biodistribution of SiO2 ENPs and will provide guidance for further toxicity studies.
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Stijn
authors.initials: S
authors.lastname: Smulders
authors.affiliation: b Biomedical MRI, Department of Imaging and Pathology, KU Leuven , Leuven , Belgium .
authors.forename: Ashwini
authors.initials: A
authors.lastname: Ketkar-Atre
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Katrien
authors.initials: K
authors.lastname: Luyts
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Hanne
authors.initials: H
authors.lastname: Vriens
authors.affiliation: c INES, LITEN, University of Grenoble Alpes, MINATEC Campus , Grenoble , France .
authors.forename: Sonia De Sousa
authors.initials: Sde S
authors.lastname: Nobre
authors.affiliation: d European Radiation Synchrotron Facility , Grenoble , France .
authors.forename: Camille
authors.initials: C
authors.lastname: Rivard
authors.affiliation: e KU Leuven BIOMAT, Department of Oral Health Sciences, KU Leuven , Leuven , Belgium , and.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Van Landuyt
authors.affiliation: f Division of Soil and Water Management , KU Leuven , Leuven , Belgium.
authors.forename: Stijn
authors.initials: S
authors.lastname: Baken
authors.affiliation: f Division of Soil and Water Management , KU Leuven , Leuven , Belgium.
authors.forename: Erik
authors.initials: E
authors.lastname: Smolders
authors.affiliation: c INES, LITEN, University of Grenoble Alpes, MINATEC Campus , Grenoble , France .
authors.forename: Luana
authors.initials: L
authors.lastname: Golanski
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Manosij
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Jeroen
authors.initials: J
authors.lastname: Vanoirbeek
authors.affiliation: b Biomedical MRI, Department of Imaging and Pathology, KU Leuven , Leuven , Belgium .
authors.forename: Uwe
authors.initials: U
authors.lastname: Himmelreich
authors.affiliation: a Department of Public Health and Primary Care , Center for Environment and Health, KU Leuven , Leuven , Belgium .
authors.forename: Peter Hm
authors.initials: PH
authors.lastname: Hoet
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D052203:Ferrosoferric Oxide / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D007275:Injections, Intravenous; D007322:Instillation, Drug; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D009928:Organ Specificity; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D014018:Tissue Distribution; D056928:X-Ray Absorption Spectroscopy
chemical_list: D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide
keywords: Body distribution; ICP-MS; MRI; XRF; silicon dioxide