title: Light up Live Cell Nuclear Envelope in Real-Time Using a Two-Photon Absorption and AIE Chromophore.
abstract: In this letter, aggregation from two-photon absorption (2PA) molecules in living cells were firstly observed and the related aggregation induced emission (AIE) properties were investigated as a cell tracer for L ((Z)-3-(4-(Bis(4-ethoxyphenyl) amino)phenyl)-2-(4-amino-phenyl)- acrylonitrile cyano-substituted ) based on triphenylamine with D-π-A model. L was further used as a two-photon absorption (2PA, λex = 900, λem = 550 nm δ = 156 GM) live-cell marker for real-time, long-term cell growth and proliferation monitoring, with rapidly adhering whole intracellular membrane-rich system. Remarkably, different from existing organic AIE chromophores and other commercially available probes, L exhibited intense intracellular-AIE property with stable nuclear envelope (NE) staining under two-photon excited microscopy (TPEM) through detailed in cellulo studies.
authors.affiliation: School of Life Science, Anhui University, Hefei, 230601, People's Republic of China. t.xiaohe@ucl.ac.uk.|Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China. t.xiaohe@ucl.ac.uk.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, MRC Center for Medical Molecular Virology, University College London, London, WC1H 0AJ, UK.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Guan
authors.affiliation: Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Zhou
authors.affiliation: School of Life Science, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Life Science, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Bei
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China. yptian@ahu.edu.cn.
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Tian
mesh_terms: D049109:Cell Proliferation; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009685:Nuclear Envelope / Q000737:chemistry*; D017785:Photons; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes
keywords: Aggregation induced emission; Live-cell image; Nuclear envelope; Real-time; Two-photon absorption