title: Anticancer effect of ethanol Lycium barbarum (Goji berry) extract on human breast cancer T47D cell line.
abstract: The anticancer activity of ethanol extract isolated from Goji berry (EEGB) on T47D human breast cancer cell line has been reported. Cell viability and cell proliferation were examined with the use of BrdU, MTT and NR methods. Induction of apoptosis was assessed by propidium iodide and Hoechst 33342 staining. Expression of genes involved in cell proliferation, apoptosis, cell cycle control and regulation of transcription was estimated using Western blotting analysis. EEGB inhibited the proliferation of breast cancer cells in a time-, and dose-dependent manner. The study confirmed the lack of EEGB cytotoxic activity to normal human skin fibroblasts. Western blot analysis demonstrated an increase in pro-apoptotic and a decrease in anti-apoptotic proteins' expression in cells treated with the extract. Anticancer activity and lack of toxicity against normal cells indicate a chemopreventive potential of Goji berries in breast cancer treatment.
authors.affiliation: a Department of Virology and Immunology , Maria Curie-Skłodowska University , Lublin , Poland.|b Department of Biochemistry and Molecular Biology , Medical University of Lublin , Lublin , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wawruszak
authors.affiliation: a Department of Virology and Immunology , Maria Curie-Skłodowska University , Lublin , Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Czerwonka
authors.affiliation: c Department of Oncological Gynaecology and Gynaecology , Medical University of Lublin , Lublin , Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Okła
authors.affiliation: a Department of Virology and Immunology , Maria Curie-Skłodowska University , Lublin , Poland.|d Department of Medical Biology , Institute of Agricultural Medicine , Lublin , Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000502:physiology; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol; D005260:Female; D006801:Humans; D032305:Lycium / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D014158:Transcription, Genetic
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D010936:Plant Extracts; D000431:Ethanol
keywords: Goji berry; T47D cells; apoptosis; breast cancer