title: Comparative study of neural differentiation of bone marrow mesenchymal stem cells by different induction methods.
abstract: Neurogenic differentiation of bone marrow (BM) mesenchymal stem cells (MSCs) offers a new hope for patients with many neurological disorders. Several chemical inducers are employed to induce BMMSCs differentiation into nerve cells. In the present study, we compared different inducers [2-mercaptoethanol (BME), tretinoin (ATRA), dimethyl sulfoxide/butylated hydroxyanisole (DMSO/BHA), and indomethacin/3-isobutyl-1-methylxanthine (indomethacin/IBMX)] on the neurogenic differentiation of BMMSCs and aimed to identify a more efficient and safer method. The MSCs were first identified by their ability to adhere to plastic and by the expression of positive (CD44, CD90, and CD105) and negative (CD34) markers assessed by flow cytometry. The efficiency of the neurogenic differentiation was determined by assessing the mRNA and protein expression of nestin, microtubule-associated protein-2 (MAP2), neuron specific enolase (NSE), and glial fibrillary acidic protein (GFAP) by reverse transcription-polymerase chain reaction and western-blot, respectively. The effect of these inducers on cell viability was also evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. This comprehensive study shows that indomethacin/IBMX is better than BME, DMSO/BHA, and ATRA both in terms of efficiency and safety, while BME suppressed the growth and proliferation of MSCs.
authors.affiliation: Department of Orthopedics, Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing, China.
authors.forename: M W
authors.initials: MW
authors.lastname: Mu
authors.affiliation: Department of Orthopedics, Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing, China lichungen_bj@126.com.
authors.forename: C G
authors.initials: CG
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D016298:Hematopoietic Cell Growth Factors; D002083:Butylated Hydroxyanisole; D014212:Tretinoin; D008623:Mercaptoethanol; D007213:Indomethacin; D004121:Dimethyl Sulfoxide