title: Effects of caffeic acid phenethyl ester on matrix molecules and angiogenetic and anti-angiogenetic factors in gastric cancer cells cultured on different substrates.
abstract: Migration, invasion, metastasis and angiogenesis associated with cancer depend on the surrounding microenvironment. Angiogenesis, the growth of new capillaries, is a regulator of cancer growth and a useful target for cancer therapy. We examined matrix protein interactions in a gastric cancer cell culture that was treated with different doses of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE). We also investigated the relations among the levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), endostatin (ES) and trombospondin-1 (TSP-1). Cytotoxity of CAPE was measured using the 3-(4,5-dmethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. We examined the behavior of cells on laminin and collagen I coated surfaces in response to the angiogenic effect of these matrix molecules. We examined the protein alterations of these matrix molecules immunohistochemically and measured the levels of VEGF, MMP-9, ES and TSP-1 using the ELISA test. We showed that application of CAPE to the gastric cancer cell line on tissue culture plastic, laminin and collagen I significantly decreased the VEGF and MMP-9 protein levels. We found that TSP-1 levels were increased significantly in the gastric cancer cells after application of CAPE. The protein levels of gastric cancer cells also were increased significantly when tissue was cultured on laminin and collagen I. Application of CAPE to cells on laminin or collagen I coated surfaces significantly increased all of the proteins except ES. ES levels were increased on the collagen I covered surfaces, but the laminin surface decreased the levels of ES significantly. We demonstrated the beneficial effect of CAPE on a gastric cancer cell line including inhibition of proliferation and induction of some proteins that might be related to decreased angiogenesis.
authors.affiliation: a Celal Bayar University School of Health , Manisa , Turkey.
authors.forename: F
authors.initials: F
authors.lastname: Kosova
authors.affiliation: b Department of Biology of Celal Bayar University Science and Art Faculty , Manisa , Turkey.
authors.forename: F O
authors.initials: FO
authors.lastname: Kurt
authors.affiliation: c Department of Pharmacology of Celal Bayar University Medical Faculty , Manisa , Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Olmez
authors.affiliation: d Department of Histology and Embryology of Celal Bayar University Medical Faculty , Manisa , Turkey.
authors.forename: I
authors.initials: I
authors.lastname: Tuğlu
authors.affiliation: e Department of Medical Biochemistry of Celal Bayar University Medical Faculty , Manisa , Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Arı
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry*; D020533:Angiogenesis Inhibitors / Q000737:chemistry*; D002109:Caffeic Acids / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D043925:Angiogenesis Inducing Agents; D020533:Angiogenesis Inhibitors; D002109:Caffeic Acids; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol
keywords: MMP-9; VEGF; angiogenesis; caffeic acid; endostatin; gastric cancer; thrombospondin-1