title: [Structure, Immunogenicity and Clinical Value of Chlamydiaphage Capsid Protein 3].
abstract: We wished to assess the role of chlamydia micro virus capsid protein Vp3 in recombinant molecules, chart its molecular evolution, screen the wild-type strain, and reveal its value in clinical research. Using a protein BLAST multiple-alignment program, we compared various strains of Chlamydia micro virus capsid protein Vp3 sequences. Using a "distance tree" of those results, we created a phylogenetic tree. We applied the Karplus-Schulz method of flexible-region analyses for highly conserved alignments of amino-acid sequences. Gamier-Robson and Chou-Fasman methods were employed to analyze two-level structures of sequences. The Emini method was used for analyses of the accessibility of surface epitopes. Studies of hydrophilic proteins were undertaken using Kyte-Doolittle and Hopp-Woods methods. Analyses of antigen epitopes helped to reveal the antigen index using the Jameson-Wolf method. All sequences in the six strains of chlamydia micro virus capsid protein Vp3 were highly conserved, with the main differences being between Vp3 protein in Chp1 and the other five strains of the micro virus. The viral strain of Vp3 protein was based mainly on micro-alpha helix structures, and multiple epitopes were noted in highly conserved regions. Vp3 protein was highly conserved structurally, and was an important protein of the chlamydiaphage capsid. Vp3 protein has a complicated molecular structure, highly conserved regions with strong immunogenicity, and has considerable research value.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yao
authors.forename: Yiju
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Qunyan
authors.initials: Q
authors.lastname: Li
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Song
authors.forename: Guiling
authors.initials: G
authors.lastname: Lu
authors.forename: Litao
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D002689:Chlamydia / Q000235:genetics / Q000276:immunology*; D017124:Conserved Sequence; D018604:Epitope Mapping; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D036022:Capsid Proteins