title: Involvement of the Arp2/3 complex and WASP proteins in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages.
abstract: The Fura-2AM fluorescent Ca(2+) probe was used to study the possibility that the Arp2/3 complex and WASP proteins are involved in the effects of glutoxim and molixan on the intracellular Ca(2+) concentration in macrophages. It has been demonstrated that preincubation of macrophages with inhibitors of the Arp2/3 complex or WASP proteins (CK-0944666 or wiskostatin, respectively) results in a significant suppression of Ca(2+)-responses induced by glutoxim or molixan. This suggests that polymerization of actin filaments is a process involved in the effect of glutoxim or molixan on intracellular Ca(2+) concentration in macrophages.
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia. zk@bio.spbu.ru.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaya
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Milenina
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Naumova
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Butov
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
authors.affiliation: St. Petersburg State University, St. Petersburg, 199034, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Nozdrachev
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002227:Carbazoles / Q000494:pharmacology; D002413:Cations, Divalent / Q000378:metabolism; D002478:Cells, Cultured; D004338:Drug Combinations; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D005978:Glutathione / Q000031:analogs & derivatives; D007211:Indoles / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D011412:Propanolamines / Q000494:pharmacology; D017208:Rats, Wistar; D056969:Voltage-Sensitive Dye Imaging; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D051376:Actin-Related Protein 2-3 Complex; C543733:CK-0944666; D002227:Carbazoles; D002413:Cations, Divalent; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007211:Indoles; D009842:Oligopeptides; D011412:Propanolamines; D051300:Wiskott-Aldrich Syndrome Protein Family; C462699:glutoxim; C489942:wiskostatin; C049925:fura-2-am; D007288:Inosine; D005978:Glutathione; D002118:Calcium; D016257:Fura-2