title: A case of spontaneous myocardial necrosis and cerebral ischemic lesions in a laboratory beagle dog.
abstract: A beagle dog treated with saline as a control animal in a preclinical study was euthanized due to sudden systemic deterioration. On histopathological examination, contraction band necrosis of myocardial cells was observed widely in the left ventricular wall, including the papillary muscle and apex, and observed slightly in the ventricular septum and left atrium. In the brain, necrosis was observed in neurons and glia of the cerebral cortex, hippocampal pyramidal cells, glial cells of the rostral commissure and Purkinje cells of the cerebellar vermis. It is highly probable that the marked systemic deterioration was caused by cardiac dysfunction due to the spontaneous contraction band necrosis of the myocardial cells, although the pathogenesis of the myocardial lesions remains unclear. Given the distribution of neuronal necrosis in the brain, it is likely that these lesions resulted from the ischemia responsible for acute cardiac failure.
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kohara
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kitaura
authors.affiliation: Department of Toxicology, Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawachi-cho, Tokushima City, Tokushima 771-0192, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsumoto
keywords: beagle dog; contraction band necrosis; ischemic brain lesion; spontaneous lesion