title: Reduction of enterotoxin induced fluid accumulation in ileal loops of neonatal calves with anti-F5 fimbriae recombinant antibody.
abstract: Neonatal calf colibacillosis caused by enterotoxigenic Escherichia coli (ETEC) is an economically significant problem in most parts of the world. The most common ETEC found in calves express the F5 (K99) fimbriae, which are necessary for the attachment of the bacteria to the ganglioside receptors on enterocytes. It is known that prevention of ETEC F5(+) adhesion to its ganglioside receptors with specific antibodies protects calves from colibacillosis. Previously we have described the development and characterization of a mouse recombinant antibody fragment (moRAb) that prevents F5 fimbrial protein induced agglutination of horse red blood cells (HRBC), which exhibit the same gangloside receptor for F5 fimbriae. Here we demonstrate that this recombinant antibody fragment inhibits in vitro the attachment of ETEC F5(+) bacteria to HRBC as well as isolated calf enterocytes, and in vivo it decreases fluid accumulation in intestinal loops of calves. Thus, correct oral administration of this anti-F5 moRAb may serve as an immunoprophylactic for cost effective control of colibacillosis in calves.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México. sahagun@unam.mx.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Sahagun-Ruiz
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México.
authors.forename: Leticia V
authors.initials: LV
authors.lastname: Velazquez
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.|Biology Department, Lone Star College, Houston, TX, 77381, USA.
authors.forename: Shoba
authors.initials: S
authors.lastname: Bhaskaran
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.|Strike Bio, Carrollton, TX, 75006, USA.
authors.forename: Chris M
authors.initials: CM
authors.lastname: Jay
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco Coyoacán, CP, 04510, México D.F., México.
authors.forename: E
authors.initials: E
authors.lastname: Morales-Salinas
authors.affiliation: Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX, 77843-2123, USA.
authors.forename: Keerti
authors.initials: K
authors.lastname: Rathore
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
authors.forename: Gale G
authors.initials: GG
authors.lastname: Wagner
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA.
authors.forename: Suryakant D
authors.initials: SD
authors.lastname: Waghela
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D020895:Enterocytes / Q000187:drug effects*; D004768:Enterotoxins / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000473:pathology; D006736:Horses; D007082:Ileum / Q000473:pathology; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins
keywords: Adhesion: calf intestinal loops; Bovine enterocytes; Enterotoxigenic Escherichia coli; Fimbriae F5; Recombinant antibody; Single-chain variable fragment