title: Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression.
abstract: The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.
authors.affiliation: Laboratório de Bioquímica e Biologia Molecular, Instituto de Ciências Exatas e Biológicas, Núcleo de Pesquisa em Biologia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: Walmir
authors.initials: W
authors.lastname: da Silva
authors.affiliation: Laboratório de Fisiologia, Departmento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Robson Augusto Souza
authors.initials: RA
authors.lastname: dos Santos
authors.affiliation: Laboratório de Biologia Molecular, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista "Júlio de Mesquita Filho", Rio Claro, SP, 13506-900, Brazil. karenmoraes_33@hotmail.com.
authors.forename: Karen C M
authors.initials: KC
authors.lastname: Moraes
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D007249:Inflammation / Q000235:genetics*; D035683:MicroRNAs / Q000502:physiology*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D051381:Rats
chemical_list: C000611830:MIRN351 microRNA, rat; D035683:MicroRNAs; D000804:Angiotensin II; D051059:PTEN Phosphohydrolase; C487216:Pten protein, rat
keywords: Angiotensin II; Cell culture; PTEN; Post-transcriptional regulation; miRNA