title: Synergistic effect of copper and low temperature over Listeria monocytogenes.
abstract: The capacity to grow at low temperatures has allowed Listeria monocytogenes to become one of the primary food pathogens to date, representing a major public health problem worldwide. Several works have described the homeostatic response of L. monocytogenes under different copper (Cu) treatments growing at mild temperature (30 °C). The aims of this report were to evaluate if changes in the external concentration of Cu affected viability and Cu homeostasis of L. monocytogenes growing at low temperature. Ours results showed that L. monocytogenes growing at 8 °C had a reduced viability relative to 30 °C when exposed to Cu treatments. This decrease was correlated with an increase in the internal concentration of Cu, probably linked to the transcriptional down-regulation of mechanisms involved in Cu homeostasis. This combined effect of Cu and low temperature showed a synergistic impact over the viability and homeostasis of L. monocytogenes, where low temperature exacerbated the toxic effect of Cu. These results can be useful in terms of the use of Cu as an antibacterial agent.
authors.affiliation: Laboratorio de Bioinformática y Expresión Génica, INTA, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile.|Center of Genome Regulation (Fondap 15090007), Universidad de Chile, Blanco Encalada 2085, Santiago, Chile.|Mathomics, Center for Mathematical Modeling, Universidad de Chile, Beauchef 851, 6th Floor, Santiago, Chile.|Center of Mathematical Modeling, Universidad de Chile, Beauchef 851, Santiago, Chile.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Latorre
authors.affiliation: Laboratorio de Microbiología y Probióticos, INTA, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Quesille-Villalobos
authors.affiliation: Laboratorio de Microbiología y Probióticos, INTA, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile.
authors.forename: Felipe
authors.initials: F
authors.lastname: Maza
authors.affiliation: Laboratorio de Microbiología y Probióticos, INTA, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile.
authors.forename: Angel
authors.initials: A
authors.lastname: Parra
authors.affiliation: Laboratorio de Microbiología y Probióticos, INTA, Universidad de Chile, El Líbano 5524, Macul, Santiago, Chile. areyes@inta.uchile.cl.
authors.forename: Angélica
authors.initials: A
authors.lastname: Reyes-Jara
mesh_terms: D002413:Cations, Divalent; D003080:Cold Temperature; D015169:Colony Count, Microbial; D003300:Copper / Q000378:metabolism*; D019327:Copper Sulfate / Q000494:pharmacology*; D017136:Ion Transport; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects
chemical_list: D002413:Cations, Divalent; D003300:Copper; D019327:Copper Sulfate
keywords: Copper; Listeria monocytogenes; Low temperature; Synergistic effect