title: The correlation of cumulus mucification patterns with oocyte maturation rate in vitro in FSH + LH-primed IVM cycles: a prospective study.
abstract: OBJECTIVE
  This prospective study was conducted to compare the oocyte maturation rate in vitro among four types of cumulus cell (CC) morphology, in dual gonadotropin-primed in vitro maturation (IVM) oocyte cycles.
  
  
  METHODS
  Two-hundred and thirty cumulus-oocyte complexes (COCs) were retrieved from FSH + hCG-primed in vitro maturation cycles of 20 patients diagnosed with polycystic ovarian syndrome. The COCs that contained immature oocytes were classified into four groups according to their cumulus mucification patterns (dispersed, clumped, compacted or sparse) and their oocyte maturation rates were compared. Chi square test and Fisher's exact test were used as appropriate.
  
  
  RESULTS
  Oocytes enclosed by dispersed and clumped CCs exhibited higher maturation rates than those with compacted and sparse patterns (P < 0.001).
  
  
  CONCLUSIONS
  In dual gonadotropin-primed IVM cycles, oocyte maturation rates are highest in those showing dispersed and clumped CC patterns.
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand. isarin.tha@mahidol.ac.th.
authors.forename: Isarin
authors.identifier: http://orcid.org/0000-0002-3912-1382
authors.initials: I
authors.lastname: Thanaboonyawat
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
authors.forename: Orawan
authors.initials: O
authors.lastname: Makemaharn
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
authors.forename: Somsin
authors.initials: S
authors.lastname: Petyim
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
authors.forename: Pitak
authors.initials: P
authors.lastname: Laokirkkiat
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Wanglang Road, Bangkoknoi, Bangkok, 10700, Thailand.
authors.forename: Roungsin
authors.initials: R
authors.lastname: Choavaratana
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D054885:Cumulus Cells; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006062:Gonadotropins; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009866:Oogenesis; D011085:Polycystic Ovary Syndrome / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D011446:Prospective Studies
chemical_list: D006062:Gonadotropins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Cumulus mucification pattern; Cumulus-oocyte complex; FSH priming; In vitro maturation; Oocyte maturation; Polycystic ovarian syndrome