title: siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
abstract: Small interfering RNA (siRNA)-mediated gene silencing has shown prominent therapeutic effects in treating various diseases. However, adequate delivery and persistent gene silencing remain challenging. A nanoparticle-based delivery system which assembled by layering siRNAs between protease degradable polypeptides to show ultra-long gene silencing effect in vivo is developed. Gold nanoparticle is used as a scaffold for its unique properties including uniform size, biocompatibility, ready synthesis, and easy functionalization. A simple layer-by-layer fabrication approach, based on the electrostatic interaction between positively and negatively charged polymers, is applied to package the therapeutic siRNAs.
authors.affiliation: Department of Radiology, Molecular Imaging Innovations Institute, Weill Cornell Medical College, New York, NY, 10021, USA.
authors.forename: Seung Koo
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Radiology, Molecular Imaging Innovations Institute, Weill Cornell Medical College, New York, NY, 10021, USA. cht2018@med.cornell.edu.
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tung
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015870:Gene Expression; D020868:Gene Silencing; D006046:Gold / Q000737:chemistry; D064593:Heterografts; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D023421:Models, Animal; D057054:Molecular Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D006046:Gold
keywords: Electrostatic interaction; Gene silencing; Gene therapy; Gold nanoparticles; In vivo; Layer-by-layer; Poly-L-lysine; Small interfering RNA; Ultra-long