title: Celiac disease: role of intestinal compartments in the mucosal immune response.
abstract: Different approaches have been used to study the pattern of cytokines in celiac disease (CD). Laser capture microdissection (LCM) is a powerful tool for the isolation of specific tissue compartments. We aimed to investigate the mucosal immune response that takes place in different intestinal compartments of CD patients, dissected by LCM, analyzing cytokine expression profile. Frozen section of jejunum was obtained from 15 untreated CD and 15 control. Surface epithelium and lamina propria compartment were isolated by LCM. RNA from each LCM sample was extracted and, after a retrotranscription step, messenger RNA levels for MxA, IL-15, TNF-α, IFN-γ, IL-17α, IL-21, IL-10, and TGF-β were determined by quantitative reverse transcriptase-PCR. Increased gene expression levels of MxA, IL-15, TNF-α, IL-10, and TGF-β was observed in the surface epithelium of untreated CD with respect to control. Furthermore, all the cytokines investigated were upregulated in the lamina propria of untreated CD as compared to control. Within the untreated CD group the expression of IL-15 was higher, in the surface epithelium than in the lamina propria, whereas the expression levels of IL-17 and IL-21 were higher in the lamina propria than in the surface epithelium. Finally, high levels of IL-10 and TGF-β were detected in both compartments of untreated CD biopsies. In CD, surface epithelium and lamina propria compartments, play a prominent role in determining innate and adaptive immunity, respectively. Conversely, surface epithelium and lamina propria produce high levels of anti-inflammatory cytokines, suggesting that both compartments are involved in the immunoregulatory response.
authors.affiliation: Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iacomino
authors.affiliation: Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Marano
authors.affiliation: Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Stillitano
authors.affiliation: Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy.
authors.forename: Vera Rotondi
authors.initials: VR
authors.lastname: Aufiero
authors.affiliation: Gastroenterology and Digestive Endoscopy Service, S. Rita Clinic, via Appia, 83042, Atripalda, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Iaquinto
authors.affiliation: Gastroenterology and Digestive Endoscopy Service, San G. Moscati Hospital, Contrada Amoretta, 83100, Avellino, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Schettino
authors.affiliation: Department of Preventive Medicine San G. Moscati Hospital, Contrada Amoretta, 83100, Avellino, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Masucci
authors.affiliation: Department of Translational Medicine-Pediatric Section, University of Naples "Federico II", Naples, Italy.|European Laboratory for the Investigation of Food-Induced Disease (ELFID), University of Naples "Federico II", via Pansini, 80131, Naples, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Troncone
authors.affiliation: Department of Translational Medicine-Pediatric Section, University of Naples "Federico II", Naples, Italy.|European Laboratory for the Investigation of Food-Induced Disease (ELFID), University of Naples "Federico II", via Pansini, 80131, Naples, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Auricchio
authors.affiliation: Institute of Food Sciences, CNR, via Roma 64, 83100, Avellino, Italy. gmazzarella@isa.cnr.it.|European Laboratory for the Investigation of Food-Induced Disease (ELFID), University of Naples "Federico II", via Pansini, 80131, Naples, Italy. gmazzarella@isa.cnr.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mazzarella
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002446:Celiac Disease / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Adaptive immunity; Celiac disease; Cytokines; Innate immunity; Intestinal compartments; Laser capture microdissection