title: Immunohistochemical localization of progesterone receptor isoforms and estrogen receptor alpha in the chicken oviduct magnum during development.
abstract: In this work, the immunohistochemical expression of progesterone receptor (PR) isoforms and estrogen receptor alpha (ER-α), as well as the histomorphometric changes of the magnum region of the left oviduct from 8-day-old chicken embryos to one-month-old chickens were evaluated. Results indicate evident histological changes in the oviduct magnum during development mainly in the magnum's mucosa. Immunohistochemical analysis showed that the oviduct magnum from 8-day-old chicken embryos to one-day-old chickens did not present any PR isoform, but the oviduct magnum of one-week and one-month-old chickens expressed PR in the nuclei of all cell types. In epithelial cells, PR-B was the only isoform expressed; in muscle and serosa cells, PR-A isoform was the only isoform expressed; and stromal cells expressed both isoforms. The results also demonstrate positive ER-α immunostaining in the nuclei of different cells from embryonic life to later developmental stages of the oviduct magnum. Data indicate that the variations of ER-α or PR expression or dominance of either PR expression is differentially regulated depending on the cell type, the development of the oviduct, and in an age-specific manner. These variations in sex steroids hormone receptors are related with histological changes of the oviduct magnum through development.
authors.affiliation: Facultad de Ciencias, Laboratorio de Biología de la Reproducción Animal, Departamento de Biología Comparada, Universidad Nacional Autónoma de México, Mexico, D.F. 04510, Mexico. Electronic address: bita@live.com.mx.
authors.forename: María Genoveva
authors.initials: MG
authors.lastname: González-Morán
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D010057:Oviducts / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D021381:Protein Transport; D011980:Receptors, Progesterone / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D047628:Estrogen Receptor alpha; D020033:Protein Isoforms; D011980:Receptors, Progesterone
keywords: Chicken; Estrogen receptor; Oviduct magnum; Progesterone receptor isoforms