title: Clinical relevance of preformed IgG and IgM antibodies against donor endothelial progenitor cells in recipients of living donor kidney grafts.
abstract: BACKGROUND
  Literature reports suggest that non-HLA-antibodies against human endothelial progenitor cells (EPC) can be detected in pre-transplant recipient serum and that EPC antibodies can have a deleterious influence on the graft.
  
  
  METHODS
  We investigated 71 renal transplant recipients from living donors for a possible influence of pre-transplant donor-specific IgG and/or IgM recipient antibodies against EPC of the donor using the flow cytometric XM-ONE cross-match.
  
  
  RESULTS
  Eight of the 71 patients developed acute biopsy-proven rejection. Two of these patients showed IgM antibodies against EPC prior to transplantation while the other six patients had neither IgG nor IgM EPC antibodies. Conversely, pre-transplant IgG or IgM antibodies against EPC were detected in 19 patients without acute rejection (3 × both IgG and IgM, 1 × IgG and 15 × IgM). The remaining 44 patients had neither EPC antibodies nor experienced rejection. Comparing serum creatinine levels at one month and one yr post-transplant within and among the three patient groups revealed that serum creatinine levels were similar in patients with or without EPC antibodies (p > 0.05).
  
  
  CONCLUSIONS
  In this series of 71 recipients with living donor kidneys, pre-transplant EPC antibodies detected with the XM-ONE test kit were neither associated with acute rejection nor with graft function at one month or one yr.
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Daniel
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Sadeghi
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Caner
authors.initials: C
authors.lastname: Suesal
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Scherer
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Hien
authors.initials: H
authors.lastname: Tran
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Gombos
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Karina
authors.initials: K
authors.lastname: Trojan
authors.affiliation: Department of Nephrology, University Hospital, Heidelberg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Morath
authors.affiliation: Department of Transplantation-Immunology, University Hospital, Heidelberg, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Opelz
mesh_terms: D000328:Adult; D066026:Endothelial Progenitor Cells / Q000276:immunology*; D005260:Female; D005500:Follow-Up Studies; D005919:Glomerular Filtration Rate; D006084:Graft Rejection / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology; D006085:Graft Survival; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D007676:Kidney Failure, Chronic / Q000601:surgery; D007677:Kidney Function Tests; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications; D011379:Prognosis; D012307:Risk Factors; D066027:Transplant Recipients
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies
keywords: XM-ONE; antibodies against endothelial progenitor cells; graft dysfunction; living donor kidney grafts; preformed EPC antibodies; rejection