title: Serial full-thickness excision of dysplastic vocal fold leukoplakia: Diagnostic or therapeutic?
abstract: OBJECTIVE
  A previous study proposed that serial full-thickness excisional biopsies of vocal fold leukoplakia therapeutically decreased dysplasia grade. The current investigation aimed to 1) analyze the pathological evolution and natural history of these lesions and 2) re-examine the role of serial excisions in dysplasia grade regression in long-term follow-up.
  
  
  METHODS
  Retrospective case series.
  
  
  METHODS
  Patients treated for vocal fold dysplasia (1994-2013) with serial full-thickness microflap-type excisions were identified and followed longitudinally. Excluded were those with one excision, invasive cancer at initial excision, or history of laryngeal cancer or radiation. Data from surgical procedures, associated pathology, and patient characteristics were recorded. Weighted repeated measures ordinal logistic regression measured associations with pathology findings.
  
  
  RESULTS
  Of 55 patients (median age = 65 years, interquartile range = 54-73 years, 89% male, 63% ever smokers, 27% alcohol users), 31 met inclusion criteria. During the study period, patients had two to 44 excisions, with a median time between excisions of 4.0 months. Each additional excision increased odds of higher-grade pathology by 4% (odds ratio = 1.04, 95% confidence interval = 1.01-1.06; P = .007). A transition model demonstrated that patients with moderate dysplasia, severe dysplasia, or carcinoma in situ on a prior biopsy had 2.64-, 5.64-, and 8.73-times increased odds of the same or higher pathology grade at the next excision, respectively.
  
  
  CONCLUSIONS
  Data do not support the hypothesis that serial full-thickness excisions decrease dysplasia grade. Progression of dysplasia appears to be nonlinear, but higher-grade dysplasia is more likely to progress to malignancy.
  
  
  METHODS
  4.
authors.affiliation: Bobby Alford Department of Otolaryngology, Baylor College of Medicine, Houston, Texas, U.S.A.
authors.forename: Annie
authors.initials: A
authors.lastname: Ahn
authors.affiliation: Department of Biostatistics.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biostatistics.
authors.forename: James C
authors.initials: JC
authors.lastname: Slaughter
authors.affiliation: Vanderbilt Voice Center, Department of Otolaryngology.
authors.forename: Amy M
authors.initials: AM
authors.lastname: Nguyen
authors.affiliation: Vanderbilt Voice Center, Department of Otolaryngology.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Ossoff
authors.affiliation: Vanderbilt Voice Center, Department of Otolaryngology.|Center for Surgical Quality and Outcomes Research, Institute for Medicine and Public Health, Vanderbilt University Medical Center, Nashville, Tennessee, U.S.A.
authors.forename: David O
authors.initials: DO
authors.lastname: Francis
mesh_terms: D000368:Aged; D000465:Algorithms; D001706:Biopsy; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000473:pathology / Q000601:surgery; D007828:Laryngoscopy; D007971:Leukoplakia / Q000473:pathology* / Q000601:surgery*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000473:pathology* / Q000601:surgery*; D012189:Retrospective Studies; D014827:Vocal Cords / Q000473:pathology* / Q000601:surgery*
keywords: Vocal fold leukoplakia; biopsy; diagnosis; dysplasia; progression; treatment