title: Associations of Leu72Met Polymorphism of Preproghrelin with Ratios of Plasma Lipids Are Diversified by a High-Carbohydrate Diet in Healthy Chinese Adolescents.
abstract: OBJECTIVE
  The association of preproghrelin Leu72Met polymorphism with plasma lipids profile was inconsistently reported and needs more studies to be confirmed. Our study was to investigate the changes of plasma lipids ratios after a high-carbohydrate (high-CHO) diet in healthy Chinese adolescents with different genotypes of this polymorphism.
  
  
  METHODS
  Fifty-three healthy university students were given a washout diet of 54.1% carbohydrate for 7 days, followed by a high-CHO diet of 70.1% carbohydrate for 6 days. The anthropometric and biological parameters were analyzed at baseline and before and after the high-CHO diet.
  
  
  RESULTS
  When compared with those before the high-CHO diet, body mass index (BMI) decreased in the male and female Met72 allele carriers. Decreased low-/high-density lipoprotein cholesterol (LDL-C/HDL-C) was observed in all participants except the female subjects with the Leu72Leu genotype. TG/HDL-C and log (TG/HDL-C) were increased only in the female subjects with the Leu72Leu genotype.
  
  
  CONCLUSIONS
  These results suggest that the Met72 allele of preproghrelin Leu72Met polymorphism may be associated with decreased BMI induced by the high-CHO diet in male and female adolescents, while the Leu72 allele with increased TG/HDL-C and log (TG/HDL-C) in the female adolescents only. Furthermore, the decreasing effect of the high-CHO diet on LDL/HDL-C may be eliminated in the female Leu72Leu homozygotes.
authors.affiliation: Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, PR China.
authors.forename: Mi
authors.initials: M
authors.lastname: Su
authors.forename: Li
authors.initials: L
authors.lastname: Qiu
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Xiao Juan
authors.initials: XJ
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.forename: Ding Zhi
authors.initials: DZ
authors.lastname: Fang
mesh_terms: D000328:Adult; D002681:China; D004032:Diet / Q000379:methods*; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D005260:Female; D054439:Ghrelin / Q000235:genetics*; D006801:Humans; D007930:Leucine; D008055:Lipids / Q000097:blood* / Q000235:genetics; D008297:Male; D008715:Methionine; D011110:Polymorphism, Genetic / Q000235:genetics*; D055815:Young Adult
chemical_list: D004040:Dietary Carbohydrates; D054439:Ghrelin; D008055:Lipids; D008715:Methionine; D007930:Leucine