title: Decreased Splenic CD4(+) T-Lymphocytes in Apolipoprotein M Gene Deficient Mice.
abstract: Spleen T-lymphocytes, especially CD4(+) T-cells, have been demonstrated to be involved in broad immunomodulation and host-defense activity in vivo. Apolipoprotein M gene (apoM) may have an important role in the regulation of immunoprocess and inflammation, which could be hypothesized to the apoM containing sphingosine-1-phosphate (S1P). In the present study we demonstrate that the splenic CD4(+) T-lymphocytes were obviously decreased in the apoM gene deficient (apoM(-/-)) mice compared to the wild type (apoM(+/+)). Moreover, these mice were treated with lipopolysaccharide (LPS) and it was found that even more pronounced decreasing CD4(+) T-lymphocytes occurred in the spleen compared to the apoM(+/+) mice. The similar phenomena were found in the ratio of CD4(+)/CD8(+) T-lymphocytes. After administration of LPS, the hepatic mRNA levels of tumor necrosis factor-α (TNF-α) and monocyte chemotactic protein-1 (MCP-1) were markedly increased; however, there were no statistical differences observed between apoM(+/+) mice and apoM(-/-) mice. The present study demonstrated that apoM might facilitate the maintenance of CD4(+) T-lymphocytes or could modify the T-lymphocytes subgroups in murine spleen, which may further explore the importance of apoM in the regulation of the host immunomodulation, although the detailed mechanism needs continuing investigation.
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Luo
authors.affiliation: Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Comprehensive Laboratory, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Hongyao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shao
authors.affiliation: Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lund University, 221 85 Lund, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Berggren-Söderlund
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lund University, 221 85 Lund, Sweden.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics*; D000075243:Apolipoproteins M; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology; D018932:Chemokine CCL2 / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D007249:Inflammation; D008070:Lipopolysaccharides / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D060888:Real-Time Polymerase Chain Reaction; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: C501623:ApoM protein, mouse; D001053:Apolipoproteins; D000075243:Apolipoproteins M; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D016207:Cytokines; D008070:Lipopolysaccharides; D008246:Lysophospholipids; D014409:Tumor Necrosis Factor-alpha; C060506:sphingosine 1-phosphate; D013110:Sphingosine