title: [The myeloid cell leukemia-1 mRNA splicing mediated by epithelial splicing regulatory protein 1 (ESRP1) in glioma U251 cell lines].
abstract: OBJECTIVE
  To investigate the regulatory mechanism of myeloid cell leukemia-1 (MCL1) mRNA splicing mediated by epithelial splicing regulatory protein 1 (ESRP1) in glioma U251 cell lines.
  
  
  METHODS
  Data mining was performed in glioma patient database to find out the key sites from MCL1 sequencing results. Exogenous ESRP1 was over-expressed in U251 cells via pcDNA-ESRP1 vector. The levels of different MCL1 isoforms were detected by reverse transcription PCR and Western blotting.
  
  
  RESULTS
  The mRNA and protein level of endogenous ESRP1 was lower in U251 cells than in normal gliocytes. The level of MCL1 isoform 1 was higher, while isoform 2 and 3 were lower in U251 cells than in normal gliocytes. Furthermore, MCL1 isoform 1 was reduced and isoform 3 was up-regulated in U251 cells after ESRP1 over-expression. Isoform 2 was unchanged obviously. Finally, MCL1 with the deletion of 801G and 802A sites could not be correctly spliced by ESRP1 and no significant difference was seen in the expressions of isoform 1 and 3 in mutant MCL1.
  
  
  CONCLUSIONS
  Repression of ESRP1 expression or mutation in its RNA recognized sites in tumor results in the abnormality of transcript splicing of oncogenes.
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Quan
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Dayuan
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Shanghai Fengxian District Central Hospital, Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 201499, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D005910:Glioma / Q000235:genetics*; D006801:Humans; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics*; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000502:physiology*
chemical_list: C539364:ESRP1 protein, human; C579095:MCL1 protein, human; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D016601:RNA-Binding Proteins