title: Dioxinodehydroeckol protects human keratinocyte cells from UVB-induced apoptosis modulated by related genes Bax/Bcl-2 and caspase pathway.
abstract: Although ultraviolet B (UVB) has a low level of skin penetration, it readily results in epidermal sunburn of keratinocytes that are destined to apoptosis after sun expose, and leads to DNA damage. Dioxinodehydroeckol (DHE), a phlorotannin from Ecklonia cava has been explored for its preventive activity against UVB-induced apoptosis in human keratinocyte (HaCaT) cells; however, the protective effects of treatment with low doses of DHE on UVB-damaged cells post-UVB exposure and their underlying mechanisms still remain unclear. The HaCaT cells were exposed to 20 mJcm(-2) of UVB irradiation which is the minimal erythema dose (MED) for individuals to be able to tan, and the expression levels of Bax/Bcl-2 and caspase-3,-8, -9 which are associated genes with apoptosis were investigated when we either treated cells with DHE doses after UVB irradiation or exposed them to UVB only. Our results suggest insight into proposed mechanistic pathway of protective activity of DHE on the HaCaT cells from UVB-induced apoptosis, indicating the benefit of DHE as a repair agent for skin damage against UVB.
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: BoMi
authors.initials: B
authors.lastname: Ryu
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Byul-Nim
authors.initials: BN
authors.lastname: Ahn
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea.
authors.forename: Kyong-Hwa
authors.initials: KH
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea; Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 617-736, Republic of Korea.
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
authors.affiliation: Marine Bioprocess Research Center, Pukyong National University, Busan 608-739, Republic of Korea; Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: sknkim@pknu.ac.kr.
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: dgkim@pknu.ac.kr.
authors.forename: Dong Gyu
authors.initials: DG
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D004147:Dioxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D014466:Ultraviolet Rays; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D004147:Dioxins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C550453:dioxinodehydroeckol; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9