title: Self-assembled mPEG-PCL-g-PEI micelles for multifunctional nanoprobes of doxorubicin delivery and magnetic resonance imaging and optical imaging.
abstract: In this paper, a novel bifunctional nanoprobe based on polyethylene glycol(MPEG)-poly(ϵ-caprolactone)(ϵ-CL)-polyethylenimine(PEI) labeled with FITC (MPEG-PCL-PEI-FITC, PCIF) were prepared to provide tumor therapy and simultaneous diagnostic information via magnetic resonance imaging (MRI) and optical imaging. Superparamagnetic iron oxide (SPIO) and doxorubicin (DOX) loaded PCIF (PCIF/SPIO/DOX) nanoprobes were prepared by self-assembling into micelles, which had uniformly distributed particle size of 130 ± 5 nm and a zeta potential of +35 ± 2 mV. Transmission electronic microscopy(TEM) showed that SPIO NPs were loaded into PCIF micelles. The PCIF/SPIO/DOX nanoprobes were superparamagnetic at 300 K with saturated magnetization of 20.5 emu/g Fe by vibrating-sample-magnetomete (VSM). Studies on cellular uptake of PCIF/SPIO/DOX nanoprobes demonstrated that SPIO NPs, DOX and FITC labeled MPEG-PCL-PEI were simultaneously taken up by the breast cancer (4T1) cells. After intravenous injection of PCIF/SPIO/DOX nanoprobes in 4T1 tumor-bearing mice, SPIO NPs, DOX and FITC labeled MPEG-PCL-PEI micelles were simultaneously delivered into tumor tissue by histochemisty. This work is important for the applications to multimodal diagnostic and theragnosis as nanomedicine.
authors.affiliation: Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang, Shandong 261061, PR China. Electronic address: qingfaguo@wfu.edu.cn.
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006,PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang, Shandong 261061, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Cao
authors.affiliation: Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang, Shandong 261061, PR China.
authors.forename: Weizhong
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Nanchang University, Nanchang, Jiangxi 330006, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
mesh_terms: D008279:Magnetic Resonance Imaging / Q000379:methods*; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D061848:Optical Imaging / Q000379:methods*; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D008823:Micelles; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; D011094:Polyethyleneimine
keywords: Doxorubicin; Magnetic resonance imaging; Nanoprobes; Optical imaging; Superparamagnetic iron oxide