title: Distinguishing of tumor cell-targeting peptide ligands through a color-encoding microarray.
abstract: A silicon-based microarray system was constructed to discover the affinity peptides and to distinguish the specific peptides from a high throughput library. Using a color-encoding strategy, in situ peptide distinguishing between HER1 ligands and HER2 ligands was achieved. Novel affinity peptide sequences H1P (HER1 ligand) and H2P (HER2 ligand) were determined with nmol affinity.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China. huzy@nanoct.cn wangwz@nanoctr.cn.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.forename: Lingling
authors.initials: L
authors.lastname: Geng
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D004867:Equipment Design; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis / Q000295:instrumentation*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D066246:ErbB Receptors; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D018719:Erb-b2 Receptor Tyrosine Kinases; C512478:EGFR protein, human; C508053:ERBB2 protein, human