title: H2S induces vasoconstriction of rat cerebral arteries via cAMP/adenylyl cyclase pathway.
abstract: Hydrogen sulfide (H2S), traditionally known for its toxic effects, is now involved in regulating vascular tone. Here we investigated the vasoconstrictive effect of H2S on cerebral artery and the underlying mechanism. Sodium hydrosulfide (NaHS), a donor of H2S, concentration-dependently induced vasoconstriction on basilar artery, which was enhanced in the presence of isoprenaline, a β-adrenoceptor agonist or forskolin, an adenylyl cyclase activator. Administration of NaHS attenuated the vasorelaxant effects of isoprenaline or forskolin. Meanwhile, the NaHS-induced vasoconstriction was diminished in the presence of 8B-cAMP, an analog of cAMP, but was not affected by Bay K-8644, a selective L-type Ca(2+) channel agonist. These results could be explained by the revised effects of NaHS on isoprenaline-induced cAMP elevation and forskolin-stimulated adenylyl cyclase activity. Additionally, NaHS-induced vasoconstriction was enhanced by removing the endothelium or in the presence of L-NAME, an inhibitor of nitric oxide synthase. L-NAME only partially attenuated the effect of NaHS which was given together with forskolin on the pre-contracted artery. In conclusion, H2S induces vasoconstriction of cerebral artery via, at least in part, cAMP/adenylyl cyclase pathway.
authors.affiliation: Department of Pharmacology, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Ping
authors.affiliation: Department of Pharmacology, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. Electronic address: leicao@mail.xjtu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Pharmacology, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
authors.forename: Yan-Ni
authors.initials: YN
authors.lastname: Mi
authors.affiliation: Department of Pharmacology, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. Electronic address: yxy@xjtu.edu.cn.
authors.forename: Yong-Xiao
authors.initials: YX
authors.lastname: Cao
mesh_terms: D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002120:Calcium Channel Agonists / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000378:metabolism; D002536:Cerebral Arteries / Q000187:drug effects* / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000378:metabolism; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: D000318:Adrenergic beta-Agonists; D002120:Calcium Channel Agonists; D020746:Calcium Channels, L-Type; D013440:Sulfides; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C044258:8-chloro-cyclic adenosine monophosphate; D000242:Cyclic AMP; D019001:Nitric Oxide Synthase; D000262:Adenylyl Cyclases; C025451:sodium bisulfide; D007545:Isoproterenol; D019331:NG-Nitroarginine Methyl Ester; D006862:Hydrogen Sulfide
keywords: Cerebral artery; Constriction; Hydrogen sulfide; Sodium hydrosulfide; cAMP; β-Adrenoceptor