title: Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells.
abstract: A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microfluidic platform, combined with image cytometry, enables the systematic analysis of multiple simultaneously detected marker expression in individual cells, for screening of various chemically defined media across hPSC lines, and the study of phenotypic responses.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 606-8501, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Mashimo
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 606-8501, Japan. kkamei@icems.kyoto-u.ac.jp.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Kamei
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation; D002460:Cell Line; D004867:Equipment Design; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation*; D008846:Micromanipulation / Q000295:instrumentation; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology*; D059010:Single-Cell Analysis / Q000295:instrumentation*
keywords: Basic fibroblast growth factor; Chemically defined media; Human pluripotent stem cells; Immunofluorescence; Matrigel; Microfluidic hPSC array; Microfluidic image cytometry