title: Quantification of reactive carbonyl compounds in icodextrin-based peritoneal dialysis fluids by combined UHPLC-DAD and -MS/MS detection.
abstract: During heat sterilization of peritoneal dialysis (PD) fluids, the glucose component is partially degraded. The formed glucose degradation products impair biocompatibility and limit the long-term application of PD fluids. As an alternative to glucose, icodextrin, a polyglucose, is used as osmotic agent in PD fluids. After targeted screening for reactive carbonyl compounds, NMR- and MS-analyses very recently revealed 4-deoxyglucosone (4-DG), 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxypentosone (3,4-DDPS), and 5-hydroxymethylfurfural (5-HMF) as main polyglucose degradation products (pGDPs) in icodextrin-based PD fluids. Now, the present study established and validated a UHPLC method with DAD as well as a UHPLC-MS/MS method for the first-time quantification of those five major pGDPs in commercial icodextrin PD fluids after derivatization with o-phenylenediamine. Thus, 4-DG was identified to be the main degradation product (in concentrations up to 20 μM). In contrast to the values measured in glucose-based products, the concentration of 3-DGal (≤ 16 μM) was higher than the concentration of 3-DG (≤ 7 μM) indicating different reaction pathways starting from polyglucose compared to glucose. The compounds 3,4-DDPS and 5-HMF were present in minor quantities (≤ 0.3 μM each).
authors.affiliation: Food Chemistry Unit, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Schuhstr. 19, 91052 Erlangen, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gensberger-Reigl
authors.affiliation: Fresenius Medical Care Deutschland GmbH, Frankfurter Str. 6-8, 66606 St. Wendel, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Huppert
authors.affiliation: Food Chemistry Unit, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Schuhstr. 19, 91052 Erlangen, Germany. Electronic address: monika.pischetsrieder@fau.de.
authors.forename: Monika
authors.initials: M
authors.lastname: Pischetsrieder
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005936:Glucans / Q000032:analysis* / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D015312:Hemodialysis Solutions / Q000032:analysis* / Q000737:chemistry; D000077607:Icodextrin; D010530:Peritoneal Dialysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005936:Glucans; D015312:Hemodialysis Solutions; D000077607:Icodextrin; D005947:Glucose
keywords: 3,4-Dideoxypentosone (3,4-DDPS); 4-Deoxyglucosone (4-DG); Icodextrin; Peritoneal dialysis; Polyglucose degradation products; α-Dicarbonyl compound