title: FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate.
abstract: The plant hormone jasmonate (JA) plays an important role in regulating growth, development, and immunity. Activation of the JA-signaling pathway is based on the hormone-triggered ubiquitination and removal of transcriptional repressors (JASMONATE-ZIM DOMAIN [JAZ] proteins) by an SCF receptor complex (SCF(COI1)/JAZ). This removal allows the rapid activation of transcription factors (TFs) triggering a multitude of downstream responses. Identification of TFs bound by the JAZ proteins is essential to better understand how the JA-signaling pathway modulates and integrates different responses. In this study, we found that the JAZ3 repressor physically interacts with the YABBY (YAB) family transcription factor FILAMENTOUS FLOWER (FIL)/YAB1. In Arabidopsis thaliana, FIL regulates developmental processes such as axial patterning and growth of lateral organs, shoot apical meristem activity, and inflorescence phyllotaxy. Phenotypic analysis of JA-regulated responses in loss- and gain-of-function FIL lines suggested that YABs function as transcriptional activators of JA-triggered responses. Moreover, we show that MYB75, a component of the WD-repeat/bHLH/MYB complex regulating anthocyanin production, is a direct transcriptional target of FIL. We propose that JAZ3 interacts with YABs to attenuate their transcriptional function. Upon perception of JA signal, degradation of JAZ3 by the SCF(COI1) complex releases YABs to activate a subset of JA-regulated genes in leaves leading to anthocyanin accumulation, chlorophyll loss, and reduced bacterial defense.
authors.affiliation: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma, 28049 Madrid, Spain.
authors.forename: Marta
authors.identifier: http://orcid.org/0000-0001-8688-5513
authors.initials: M
authors.lastname: Boter
authors.affiliation: School of BioSciences, University of Melbourne, Royal Parade, Parkville, Victoria 3010, Australia.
authors.forename: John F
authors.identifier: http://orcid.org/0000-0001-9478-5459
authors.initials: JF
authors.lastname: Golz
authors.affiliation: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma, 28049 Madrid, Spain.
authors.forename: Selena
authors.initials: S
authors.lastname: Giménez-Ibañez
authors.affiliation: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma, 28049 Madrid, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Fernandez-Barbero
authors.affiliation: Genomics Unit, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma, 28049 Madrid, Spain.
authors.forename: José M
authors.identifier: http://orcid.org/0000-0001-6769-7349
authors.initials: JM
authors.lastname: Franco-Zorrilla
authors.affiliation: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología-CSIC, Campus Universidad Autónoma, 28049 Madrid, Spain rsolano@cnb.csic.es.
authors.forename: Roberto
authors.initials: R
authors.lastname: Solano
mesh_terms: D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism*; D001665:Binding Sites; D003517:Cyclopentanes / Q000494:pharmacology*; D008954:Models, Biological; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D044224:Pseudomonas syringae / Q000187:drug effects / Q000502:physiology
chemical_list: C119103:AFO protein, Arabidopsis; D000872:Anthocyanins; D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D003517:Cyclopentanes; C522646:JAI3 protein, Arabidopsis; D054883:Oxylipins; C011006:jasmonic acid