title: CXCL10, CXCL11, HLA-A and IL-1β are induced in peripheral blood mononuclear cells from women with Chlamydia trachomatis related infertility.
abstract: Chlamydia trachomatis infections can result in the development of serious sequelae such as pelvic inflammatory disease and tubal infertility. In this study, peripheral blood mononuclear cells from women who were undergoing or had recently undergone IVF treatment were cultured ex vivo with C. trachomatis to identify the immune responses associated with women who had serological evidence of a history of Chlamydia infection. Cytokines secreted into the supernatant from the cultures were measured using ELISA, and the level of IL-1β was found to be significantly higher in Chlamydia positive women than Chlamydia negative women. qRT-PCR analysis of the expression of 88 immune-related genes showed trends towards an upregulation of CXCL10, CXCL11 and HLA-A in Chlamydia positive women compared with Chlamydia negative women. These findings support that some women launch a more marked proinflammatory response upon infection with C. trachomatis and this may be associated with why C. trachomatis induces infertility in some infected women.
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
authors.forename: Shruti
authors.initials: S
authors.lastname: Menon
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Alexander
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, QLD 4558, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Timms
authors.affiliation: Wesley and St Andrews Research Institute, The Wesley Hospital, Auchenflower, QLD 4066, Australia UC Health Clinical School, The Wesley Hospital, Auchenflower, QLD 4066, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Allan
authors.affiliation: Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD 4059, Australia Wesley and St Andrews Research Institute, The Wesley Hospital, Auchenflower, QLD 4066, Australia w.huston@qut.edu.au.
authors.forename: Wilhelmina M
authors.initials: WM
authors.lastname: Huston
mesh_terms: D000328:Adult; D001769:Blood / Q000276:immunology; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000276:immunology*; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D015234:HLA-A Antigens / Q000032:analysis*; D006801:Humans; D007246:Infertility / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000276:immunology*; D008219:Lymphogranuloma Venereum / Q000150:complications* / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D015234:HLA-A Antigens
keywords: chlamydia; infertility; pathology