title: Analysis of upland cotton (Gossypium hirsutum) response to Verticillium dahliae inoculation by transcriptome sequencing.
abstract: Verticillium wilt is one of the main diseases in cotton (Gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. At present, the molecular mechanism that confers resistance to this disease is unclear. Transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. In this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with Verticillium dahliae was sequenced. A total of 126,402 unigenes were obtained using de novo assembly and data analysis, 99,712 (78.88%) of which were annotated into the Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. The expression patterns of 16 candidate disease-resis-tance genes showed that some genes were upregulated soon after V. dahliae inoculation and others were upregulated later, which may indicate instanta-neous basal defense and lagged specific defense, respectively. We conducted a preliminary analysis of the transcriptome database, which will contribute to further research regarding the cloning of disease-resistance genes.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: B X
authors.initials: BX
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Sang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Wang
mesh_terms: D019295:Computational Biology; D060467:Disease Resistance / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D054884:Host-Pathogen Interactions / Q000235:genetics; D053858:Metabolic Networks and Pathways; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000235:genetics; D059467:Transcriptome; D020172:Verticillium