title: Effect of SERCA2a overexpression in the pericardium mediated by the AAV1 gene transfer on rapid atrial pacing in rabbits.
abstract: To study the effects of overexpression of the sarcoplasmic reticulum ATPase 2a (SERCA2a) gene on the activity and protein expression of SERCA2a after rapid atrial pacing (RAP) in New Zealand white rabbits. New Zealand white rabbits were randomly divided into a sham-operated group (group A), adeno-associated virus 1 (AAV1)/EGFP + atrial fibrillation (AF) model group (group B), or AVV1/SERCA2a + AF group (group C). The sham-operated group was used as a negative control. Each group consisted of 10 animals. Groups B and C were injected with 500 μL of the AAV1-EGFP reporter gene and 500 μL of the AAV1-SERCA2a target gene, respectively. Four weeks after AAV1-mediated gene transfer, the rabbits underwent 24 h of RAP to the right atrium. The animals were sacrificed and protein activity and protein expression in the myocardium were measured using the westernblot method. Four weeks after AAV1-mediated gene transfer, SERCA2a protein activity and expression were significantly higher in Group C than in Groups A and B (P < 0.05). RAP of the right atrium induced atrial fibrillation in rabbits, resulting in decreases in the activity and protein expression of SERCA2a. Pericardial AAV-1 mediated SERCA2a gene transfer resulted in the overexpression of SERCA2a, restoring SERCA2a activity and protein expression.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: B N
authors.initials: BN
authors.lastname: Kuken
authors.affiliation: Department of Drug Analysis, Faculty of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: A N W E
authors.initials: AN
authors.lastname: Aikemu
authors.affiliation: Intensive Care Unit, People's Hospital of Yanqi County, Yanqi County, Xinjiang, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Xiang
authors.affiliation: Comprehensive Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: M H Y T
authors.initials: MH
authors.lastname: Wulasihan
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000235:genetics / Q000628:therapy; D000229:Dependovirus / Q000235:genetics*; D004195:Disease Models, Animal; D004789:Enzyme Activation; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D006325:Heart Atria / Q000378:metabolism* / Q000503:physiopathology*; D007150:Immunohistochemistry; D010496:Pericardium / Q000378:metabolism*; D011817:Rabbits; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism
chemical_list: D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases