title: Strigolactones spatially influence lateral root development through the cytokinin signaling network.
abstract: Strigolactones are important rhizosphere signals that act as phytohormones and have multiple functions, including modulation of lateral root (LR) development. Here, we show that treatment with the strigolactone analog GR24 did not affect LR initiation, but negatively influenced LR priming and emergence, the latter especially near the root-shoot junction. The cytokinin module ARABIDOPSIS HISTIDINE KINASE3 (AHK3)/ARABIDOPSIS RESPONSE REGULATOR1 (ARR1)/ARR12 was found to interact with the GR24-dependent reduction in LR development, because mutants in this pathway rendered LR development insensitive to GR24. Additionally, pharmacological analyses, mutant analyses, and gene expression analyses indicated that the affected polar auxin transport stream in mutants of the AHK3/ARR1/ARR12 module could be the underlying cause. Altogether, the data reveal that the GR24 effect on LR development depends on the hormonal landscape that results from the intimate connection with auxins and cytokinins, two main players in LR development.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Lingxiang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Cedrick
authors.initials: C
authors.lastname: Matthys
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Belen
authors.initials: B
authors.lastname: Marquez-Garcia
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Carolien
authors.initials: C
authors.lastname: De Cuyper
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Lien
authors.initials: L
authors.lastname: Smet
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Annick
authors.initials: A
authors.lastname: De Keyser
authors.affiliation: Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique-AgroParisTech, 78026 Versailles Cedex, France Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, Unité Propre de Recherche 2301, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France.
authors.forename: François-Didier
authors.initials: FD
authors.lastname: Boyer
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Beeckman
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium Ghent University Global Campus, Incheon 406-840, Korea.
authors.forename: Stephen
authors.initials: S
authors.lastname: Depuydt
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium sofie.goormachtig@psb.vib-ugent.be.
authors.forename: Sofie
authors.initials: S
authors.lastname: Goormachtig
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003583:Cytokinins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006575:Heterocyclic Compounds, 3-Ring / Q000378:metabolism*; D000071677:Histidine Kinase; D007783:Lactones / Q000378:metabolism*; D018517:Plant Roots / Q000254:growth & development; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C117138:ARR1 protein, Arabidopsis; C519632:ARR12 protein, Arabidopsis; D029681:Arabidopsis Proteins; D003583:Cytokinins; D004268:DNA-Binding Proteins; C000591191:GR24 strigolactone; D006575:Heterocyclic Compounds, 3-Ring; D007783:Lactones; D014157:Transcription Factors; D011494:Protein Kinases; D000071677:Histidine Kinase; C000605493:AHK3 protein, Arabidopsis
keywords: Arabidopsis thaliana; cytokinin signaling; lateral root development; polar auxin transport; strigolactones.