title: Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
abstract: Microvesicles and exosomes are released to the extracellular milieu and are detectable in body fluids. They act as unique vehicles for cargo transfer to other cells/tissues. They contain a set of membrane receptors, intracellular proteins and nucleic acids. Here, we describe basic techniques for their isolation and characterization from cell culture media or body fluids. We also describe critical techniques for characterizing their cargo (miRNAs) and their protein content. Finally, we present labeling methods for their use in in vitro procedures and for their in vivo delivery and subsequent analysis of their cargo transfer to multiple cell types.
authors.affiliation: Division of Oncology, Adhesion and Metastasis Laboratory, Center for Applied Medical Research (CIMA), University of Navarra, Avda. Pío XII 55, Pamplona, 31080, Navarra, Spain.
authors.forename: Karmele
authors.initials: K
authors.lastname: Valencia
authors.affiliation: Division of Oncology, Adhesion and Metastasis Laboratory, Center for Applied Medical Research (CIMA), University of Navarra, Avda. Pío XII 55, Pamplona, 31080, Navarra, Spain. flecanda@unav.es.|IdiSNA, Navarra Institute for Health Research, Pamplona, Spain. flecanda@unav.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Lecanda
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D055252:Cell-Derived Microparticles / Q000378:metabolism* / Q000648:ultrastructure; D055354:Exosomes / Q000378:metabolism / Q000648:ultrastructure; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification; D010316:Particle Size; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs
keywords: Exosome; Microvesicle; Nanoparticle