title: Integrated analysis of microRNA and mRNA expression profiles in abdominal adipose tissues in chickens.
abstract: Excessive fat accretion is a crucial problem during broiler production. Abdominal fat weight (AbFW) and abdominal fat percentage (AbFP) are major phenotypic indices of fat traits. The present study used F2 females derived from a cross between Beijing-You and Cobb-Vantress chickens. Cohorts with extreme AbFP and AbFW phenotypes were chosen to construct high- and low-abdominal fat libraries (HAbF and LAbF, respectively) to investigate the expression profiles by RNA-sequencing and microRNA (miRNA)-sequencing. Compared with the LAbF library, 62 differentially expressed miRNAs (DEMs) and 303 differentially expressed genes (DEGs) were identified in the HAbF birds. Integrated analysis of DEMs and DEGs showed that a total of 106 DEGs were identified as target genes for the 62 DEMs. These genes were designated as intersection genes, and 11 of these genes are involved in lipid metabolism pathways. The miRNA gga-miR-19b-3p accelerated the proliferation of preadipocytes, as well as adipocyte differentiation, by down- regulating ACSL1. These findings suggest that some strong candidate miRNAs and genes, important in relation to abdominal adipose deposition, were identified by the integrated analysis of DEMs and DEGs. These findings add to our current understanding of the molecular genetic controls underlying abdominal adipose accumulation in chickens.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.|Institute of Poultry Science, Chinese Academy of Agriculture Sciences, Yangzhou 225125, P. R. China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Huang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: R R
authors.initials: RR
authors.lastname: Liu
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: G P
authors.initials: GP
authors.lastname: Zhao
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Li
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: M Q
authors.initials: MQ
authors.lastname: Zheng
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: Institute of Poultry Science, Chinese Academy of Agriculture Sciences, Yangzhou 225125, P. R. China.
authors.forename: S F
authors.initials: SF
authors.lastname: Li
authors.affiliation: Institute of Poultry Science, Chinese Academy of Agriculture Sciences, Yangzhou 225125, P. R. China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: J
authors.initials: J
authors.lastname: Wen
mesh_terms: D020413:3' Untranslated Regions; D050153:Abdominal Fat / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D053263:Gene Regulatory Networks; D050356:Lipid Metabolism / Q000235:genetics; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger; D003066:Coenzyme A Ligases