title: Cellulose-based porous scaffold for bone tissue engineering applications: Assessment of hMSC proliferation and differentiation.
abstract: Physical foaming combined with microwave-induced curing was used in this study to develop an innovative device for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (i.e. pluronic) as blowing agent of a homogeneous blend of Sodium salt of carboxymethylcellulose (CMCNa) and polyethylene glycol diacrylate (PEGDA700) solution. In the second step, the porous structure of the scaffold was chemically stabilized by radical polymerization induced by a homogeneous rapid heating of the sample in a microwave reactor. In this step 2,2-Azobis[2-(2-imidazolin-2 yl)propane]Dihydrochloride was used as thermoinitiator (TI). CMCNa and PEGDA were mixed with different blends to correlate the properties of final product with the composition. The chemical properties of each sample were evaluated by spectroscopy analysis ATR-IR (before and after curing) in order to maximize reaction yield, and optimize kinetic parameters (i.e. time curing, microwave power). The stability of the materials was evaluated in vitro by degradation test in Phosphate Buffered Saline. Biological analyses were performed to evaluate the effect of scaffold materials on cellular behavior in terms of proliferation and early osteogenic differentiation of human Mesenchymal Stem Cells. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 726-733, 2016.
authors.affiliation: Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
authors.forename: Christian
authors.initials: C
authors.lastname: Demitri
authors.affiliation: Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy Mostra D'oltremare Pad.20, Viale Kennedy 54, Naples, 80125, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Raucci
authors.affiliation: Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Giuri
authors.affiliation: Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
authors.forename: Vincenzo Maria
authors.initials: VM
authors.lastname: De Benedictis
authors.affiliation: Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy Mostra D'oltremare Pad.20, Viale Kennedy 54, Naples, 80125, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Giugliano
authors.affiliation: Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Calcagnile
authors.affiliation: Department of Engineering for Innovation, University of Salento, via Monteroni, Km 1, Lecce, 73100, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sannino
authors.affiliation: Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy Mostra D'oltremare Pad.20, Viale Kennedy 54, Naples, 80125, Italy.|Department of Chemical Sciences and Materials Technology, National Research Council of Italy (DSCTM-CNR), P.Le Aldo Moro 7, Rome, 00185, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002152:Calorimetry, Differential Scanning; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D013055:Spectrophotometry, Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C437167:poly(ethylene glycol)diacrylate; D011092:Polyethylene Glycols; D002482:Cellulose; D004247:DNA; D000469:Alkaline Phosphatase
keywords: cellulose; human mesenchymal stem cells; porous materials; scaffold; tissue engineering