title: GLUTATHIONE PEROXIDASE-1 PRO200LEU POLYMORPHISM (RS1050450) IS ASSOCIATED WITH MORBID OBESITY INDEPENDENTLY OF THE PRESENCE OF PREDIABETES OR DIABETES IN WOMEN FROM CENTRAL MEXICO.
abstract: BACKGROUND
  obesity affects more than a third of Mexican population. Oxidative stress participates actively in the etiology of this phenomenon. Glutathione peroxidase-1 (GPX-1) plays a protective role against oxidative stress. The SNP Pro200Leu (rs10504050) has been reported to affect the activity of the enzyme.
  
  
  OBJECTIVE
  to determine the frequency of rs10504050 polymorphism in women with obesity and normal weight control, asses the concentration of peripheral TBARS and evaluate the consumption of pro and antioxidants.
  
  
  METHODS
  104 women with obesity and 70 healthy controls (CG) were included in the study. Anthropometric, biochemical, clinical and dietary features were evaluated. GPx-1 rs10504050 was determined by PCR/RFLP method. TBARS was assayed spectrophotometrically in plasma. The subjects were stratified and compared by obesity grades and by subgroups of prediabetes and diabetes condition. Statistical analysis included ANOVA of Kruskal Wallis, Xi squared and Pearson correlation.
  
  
  RESULTS
  for rs10504050 polymorphism there were differences (Xi2 = 6; p = 0.01) between frequency (0.61) of obese carriers (Pro/Leu plus Leu/Leu) and CG carriers (0.42), and between (Xi2 = 8; p = 0.004) morbid (IMC > 40) obesity (0.74) and CG carriers. The obese group (OB) showed a prevalence of 66% of prediabetes plus diabetes. There were no differences in frequencies of rs10504050 in OB with pre or diabetes versus CG, or versus obese participants without diabetes. TBARS concentration was greater in all the degrees of OB versus CG.
  
  
  CONCLUSIONS
  GPx-1 Pro200Leu polymorphism was associated with obesity especially with morbid obesity, but not with obese participants with prediabetes or diabetes. Oxidative stress is present in all grades of obesity significantly.
authors.affiliation: Departamento de Salud, Universidad Iberoamericana Ciudad de México.. cesar.hernandez@ibero.mx.
authors.forename: César
authors.initials: C
authors.lastname: Hernández Guerrero
authors.affiliation: Departamento de Salud, Universidad Iberoamericana Ciudad de México.. pauihdz@hotmail.com.
authors.forename: Paulina
authors.initials: P
authors.lastname: Hernández Chávez
authors.affiliation: Departamento de Salud, Universidad Iberoamericana Ciudad de México.. macn25@hotmail.com.
authors.forename: Noemí
authors.initials: N
authors.lastname: Martínez Castro
authors.affiliation: Departamento de Salud, Universidad Iberoamericana Ciudad de México.. alicia.parra@ibero.mx.
authors.forename: Alicia
authors.initials: A
authors.lastname: Parra Carriedo
authors.affiliation: Egresada de la Maestría en Nutriología Aplicada de la Universidad Iberoamericana Ciudad de México. México.. sandra_sr@hotmail.com.
authors.forename: Sandra
authors.initials: S
authors.lastname: García Del Rio
authors.affiliation: Departamento de Salud, Universidad Iberoamericana Ciudad de México.. anabertha.perez@ibero.mx.
authors.forename: Ana
authors.initials: A
authors.lastname: Pérez Lizaur
mesh_terms: D000328:Adult; D003920:Diabetes Mellitus / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005979:Glutathione Peroxidase / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D007930:Leucine / Q000235:genetics; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009767:Obesity, Morbid / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011236:Prediabetic State / Q000453:epidemiology / Q000235:genetics*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D000094765:Glutathione Peroxidase GPX1
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D007930:Leucine; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human