title: Molecularly imprinted polymeric stir bar: Preparation and application for the determination of naftopidil in plasma and urine samples.
abstract: In this study, molecularly imprinting technology and stir bar absorption technology were combined to develop a microextraction approach based on a molecularly imprinted polymeric stir bar. The molecularly imprinted polymer stir bar has a high performance, is specific, economical, and simple to prepare. The obtained naftopidil-imprinted polymer-coated bars could simultaneously agitate and adsorb naftopidil in the sample solution. The ratio of template/monomer/cross-linker and conditions of template removal were optimized to prepare a stir bar with highly efficient adsorption. Fourier transform infrared spectroscopy, scanning electron microscopy, selectivity, and extraction capacity experiments showed that the molecularly imprinted polymer stir bar was prepared successfully. To utilize the molecularly imprinted polymer stir bar for the determination of naftopidil in complex body fluid matrices, the extraction time, stirring speed, eluent, and elution time were optimized. The limits of detection of naftopidil in plasma and urine sample were 7.5 and 4.0 ng/mL, respectively, and the recoveries were in the range of 90-112%. The within-run precision and between-run precision were acceptable (relative standard deviation <7%). These data demonstrated that the molecularly imprinted polymeric stir bar based microextraction with high-performance liquid chromatography was a convenient, rapid, efficient, and specific method for the precise determination of trace naftopidil in clinical analysis.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Deli
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.|Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, China.
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Shi
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, China.
authors.forename: Kexin
authors.initials: K
authors.lastname: Shi
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D054802:Molecular Imprinting; D009281:Naphthalenes / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D010879:Piperazines / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D009281:Naphthalenes; D010879:Piperazines; D011108:Polymers; C064357:naftopidil
keywords: Biological matrices; Microextraction; Molecularly imprinted polymers; Naftopidil; Stir bar extraction