title: Identification of a novel canine distemper virus B-cell epitope using a monoclonal antibody against nucleocapsid protein.
abstract: Canine distemper virus (CDV) is a member of the genus Morbillivirus within the family Paramyxoviridae and has caused severe economic losses in China. Nucleocapsid protein (N) is the major structural viral protein and can be used to diagnose CDV and other morbilliviruses. In this study, a specific monoclonal antibody, 1N8, was produced against the CDV N protein (amino acids 277-471). A linear N protein epitope was identified by subjecting a series of partially overlapping synthesized peptides to enzyme-linked immunosorbent assay (ELISA) analysis. The results indicated that (350)LNFGRSYFDPA(360) was the minimal linear epitope that could be recognized by mAb 1N8. ELISA assays revealed that mouse anti-CDV sera could also recognize the minimal linear epitope. Alignment analysis of the amino acid sequences indicated that the epitope was highly conserved among CDV strains. Furthermore, the epitope was conserved among other morbilliviruses, which was confirmed with PRRV using western blotting. Taken together, the results of this study may have potential applications in the development of suitable diagnostic techniques for CDV or other morbilliviruses.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Yuening
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Tong
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Jianke
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Hang
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province 130122, PR China. Electronic address: tcscsp@126.com.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D017124:Conserved Sequence; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D044967:Serum / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D019590:Nucleocapsid Proteins
keywords: B-cell epitope; Canine distemper virus; Monoclonal antibody; Nucleocapsid protein