title: High-resolution pluronic-filled microchip CE-SSCP analysis system via channel width control.
abstract: Although the resolution of CE-SSCP has been significantly improved by using a poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO; Pluronic(®)) triblock copolymer as a separation medium, CE-SSCP on a microchip format is not widely applicable because their resolution is limited by short channel length. Therefore, a strategy to improve the resolution in channels of limited lengths is highly required for enabling microchip-based CE-SSCP. In this study, we developed a high-resolution CE-SSCP microchip system by controlling the width of the pluronic-filled channel. We tested four different channel widths of 180, 240, 300, and 400 μm, and found that 300 μm showed the highest resolution in the separation of two pathogen specific markers. Potential applications of our method in various genetic analyses were also shown by using SNP markers for spinal muscular atrophy.
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.
authors.forename: Giyoung
authors.initials: G
authors.lastname: Shin
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.
authors.forename: Dong-Kyun
authors.initials: DK
authors.lastname: Kim
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.|Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Daeyeon
authors.initials: D
authors.lastname: Lee
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.|Department of Physics, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.
authors.forename: Nam Ki
authors.initials: NK
authors.lastname: Lee
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.|Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, Korea.
authors.forename: Gyoo Yeol
authors.initials: GY
authors.lastname: Jung
mesh_terms: D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D008957:Models, Genetic; D011092:Polyethylene Glycols / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D011409:Propylene Glycols / Q000737:chemistry*; D055533:Survival of Motor Neuron 1 Protein / Q000235:genetics
chemical_list: C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D055533:Survival of Motor Neuron 1 Protein; D011092:Polyethylene Glycols; D004247:DNA
keywords: CE-SSCP; Microchip; PEO-PPO-PEO triblock copolymer; Pathogen; SNP