title: A High-Fidelity Codon Set for the T4 DNA Ligase-Catalyzed Polymerization of Modified Oligonucleotides.
abstract: In vitro selection of nucleic acid polymers can readily deliver highly specific receptors and catalysts for a variety of applications; however, it is suspected that the functional group deficit of nucleic acids has limited their potential with respect to proteinogenic polymers. This has stimulated research toward expanding their chemical diversity to bridge the functional gap between nucleic acids and proteins to develop a superior biopolymer. In this study, we investigate the effect of codon library size and composition on the sequence specificity of T4 DNA ligase in the DNA-templated polymerization of both unmodified and modified oligonucleotides. Using high-throughput DNA sequencing of duplex pairs, we have uncovered a 256-membered codon set that yields sequence-defined modified ssDNA polymers in high yield and with high fidelity.
authors.affiliation: Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
authors.forename: Dehui
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
authors.forename: Ryan
authors.initials: R
authors.lastname: Hili
mesh_terms: D055162:Biocatalysis; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D058105:Polymerization
chemical_list: D001704:Biopolymers; D009841:Oligonucleotides; D011088:DNA Ligases
keywords: DNA sequencing; DNA-templated; SELEX; T4 DNA ligase; aptamers