title: Effects of esculetin on lipopolysaccharide (LPS)-induced acute lung injury via regulation of RhoA/Rho Kinase/NF-кB pathways in vivo and in vitro.
abstract: The purpose of the present study was to investigate the protective effect of esculetin (ES) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the lung epithelial A549 cells. Mice were intragastrically administered with ES (20 and 40 mg/kg) 1 h prior to LPS challenge. ES pretreatment at doses of 20 and 40 mg/kg effectively attenuated LPS-induced lung histopathological change, myeloperoxidase or MPO activity, inflammatory cells infiltration, pulmonary wet-to-dry weight ratio, and the generation of pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in vivo and in vitro. Furthermore, we demonstrated that ES blocked the activation of NF-кB and RhoA/Rho kinase pathways in LPS-induced mice and A549 cells. The results suggested that ES exhibited protective effect on ALI and might attribute partly to the inhibition of NF-кB and RhoA/Rho kinase pathways in vivo and in vitro.
authors.affiliation: a State Key Laboratory of Natural Medicines, China Pharmaceutical University , No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu Province , PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.affiliation: a State Key Laboratory of Natural Medicines, China Pharmaceutical University , No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu Province , PR China.|b Department of Physiology and Pharmacology , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Guo
authors.affiliation: c School of Pharmacy, China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: c School of Pharmacy, China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: c School of Pharmacy, China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Ciman
authors.initials: C
authors.lastname: Wang
authors.affiliation: c School of Pharmacy, China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: d Department of Respiratory Medicine , The Second Hospital of Jilin University , Changchun, 130041 , China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Meng
authors.affiliation: a State Key Laboratory of Natural Medicines, China Pharmaceutical University , No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu Province , PR China.|b Department of Physiology and Pharmacology , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Yunman
authors.initials: Y
authors.lastname: Li
authors.affiliation: a State Key Laboratory of Natural Medicines, China Pharmaceutical University , No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu Province , PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ji
authors.affiliation: a State Key Laboratory of Natural Medicines, China Pharmaceutical University , No. 24 Tongjiaxiang, Nanjing 210009, Jiangsu Province , PR China.|b Department of Physiology and Pharmacology , China Pharmaceutical University , Nanjing 210009 , China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Yan
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014468:Umbelliferones / Q000494:pharmacology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism; D054460:rho-Associated Kinases / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014468:Umbelliferones; D054460:rho-Associated Kinases; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; C007628:esculetin
keywords: Esculetin; RhoA/Rho kinase/NF-кB; acute lung injury; lipopolysaccharide (LPS); lung epithelial A549