title: Design of a dual aptamer-based recognition strategy for human matrix metalloproteinase 9 protein by piezoelectric biosensors.
abstract: MMP-9, human matrix metalloproteinase 9, belongs to the family of zinc-dependent peptide-bond hydrolases and is involved in the degradation of the extracellular matrix (ECM). In clinics, it is well known that elevated MMP-9 serum levels are associated with cardiovascular dysfunctions, several aspects of the physiology and pathology of the central nervous system, neuropsychiatric disorders and degenerative diseases related to brain tumors, and excitotoxic/neuroinflammatory processes. Due to the large interest of diagnostics in this protein, efforts to set up sensitive methods to detect MMP-9 for early diagnosis of a number of metabolic alterations are rapidly increasing. In this panorama, biosensors could play a key role; therefore we explored for the first time the development of an aptamer-based piezoelectric biosensor for a sensitive, label free, and real time detection of MMP-9. The detecting strategy involved two different aptamers in a sandwich-like approach able to detect down to 100 pg mL(-1) (1.2 pM) of MMP-9 as detection limit in standard solution. As proof of principle, commercial serum was investigated in terms of possible interferents, their identification and role in MMP-9 detection. The estimated detection limit for MMP-9 is about 560 pg mL(-1) (6.8 pM) in untreated serum.
authors.affiliation: Laboratorio Sensori e Biosensori, Dipartimento di Chimica 'Ugo Schiff', Università degli Studi di Firenze, via della Lastruccia 3, Sesto Fiorentino, 50019, Firenze, Italy. Electronic address: simona.scarano@unifi.it.
authors.forename: S
authors.initials: S
authors.lastname: Scarano
authors.affiliation: ARNA Lab. InsermU869, European Institute of Chemistry and Biology, University of Bordeaux, 2, rue Robert Escarpit, 33607, Pessac, France.
authors.forename: E
authors.initials: E
authors.lastname: Dausse
authors.affiliation: Laboratorio Sensori e Biosensori, Dipartimento di Chimica 'Ugo Schiff', Università degli Studi di Firenze, via della Lastruccia 3, Sesto Fiorentino, 50019, Firenze, Italy; Dipartimento di Scienze, Università della Basilicata, Viale dell'Ateneo Lucano 10, 85100, Potenza, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Crispo
authors.affiliation: ARNA Lab. InsermU869, European Institute of Chemistry and Biology, University of Bordeaux, 2, rue Robert Escarpit, 33607, Pessac, France.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Toulmé
authors.affiliation: Laboratorio Sensori e Biosensori, Dipartimento di Chimica 'Ugo Schiff', Università degli Studi di Firenze, via della Lastruccia 3, Sesto Fiorentino, 50019, Firenze, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Minunni
mesh_terms: D052158:Aptamers, Peptide / Q000138:chemical synthesis / Q000737:chemistry*; D015374:Biosensing Techniques; D004867:Equipment Design; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000378:metabolism
chemical_list: D052158:Aptamers, Peptide; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Aptamer; Aptasensor; Human matrix metalloproteinase 9; Human serum; Piezoelectric biosensor