title: Erythrocyte Catalase Activity in More Frequent Microcytic Hypochromic Anemia: Beta-Thalassemia Trait and Iron Deficiency Anemia.
abstract: Most common microcytic hypochromic anemias are iron deficiency anemia (IDA) and β-thalassemia trait (BTT), in which oxidative stress (OxS) has an essential role. Catalase causes detoxification of H2O2 in cells, and it is an indispensable antioxidant enzyme. The study was designed to measure erythrocyte catalase activity (ECAT) in patients with IDA (10) or BTT (21), to relate it with thalassemia mutation type (β (0) or β (+)) and to compare it with normal subjects (67). Ninety-eight individuals were analyzed since September 2013 to June 2014 in Tucumán, Argentina. Total blood count, hemoglobin electrophoresis at alkaline pH, HbA2, catalase, and iron status were performed. β-thalassemic mutations were determined by real-time PCR. Normal range for ECAT was 70,0-130,0 MU/L. ECAT was increased in 14% (3/21) of BTT subjects and decreased in 40% (4/10) of those with IDA. No significant difference (p = 0,245) was shown between normal and BTT groups, while between IDA and normal groups the difference was proved to be significant (p = 0,000). In β (0) and β (+) groups, no significant difference (p = 0,359) was observed. An altered ECAT was detected in IDA and BTT. These results will help to clarify how the catalase activity works in these anemia types.
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: Sandra Stella
authors.initials: SS
authors.lastname: Lazarte
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina ; Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Mónaco
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: Cecilia Laura
authors.initials: CL
authors.lastname: Jimenez
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: Miryam Emilse
authors.initials: ME
authors.lastname: Ledesma Achem
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: Magdalena María
authors.initials: MM
authors.lastname: Terán
authors.affiliation: Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Balcarce 747, San Miguel de Tucumán, 4000 Tucumán, Argentina.
authors.forename: Blanca Alicia
authors.initials: BA
authors.lastname: Issé