title: The Role of Ubiquitination to Determine Non-Smad Signaling Responses.
abstract: Ubiquitination is a posttranslational modification of proteins which acts as a key regulator of their function as well as fate. We have recently reported transforming growth factor β (TGFβ)-induced activation of non-Smad signaling responses through a specific Lys63-linked polyubiquitination of TGFβ type I receptor and TGFβ-associated kinase 1 (TAK1) that are utilized to specify cellular responses in cancer cells. This chapter gives a brief introduction of the biological importance of ubiquitination of proteins, the methods we have used for detecting new partners in the TGFβ signaling pathway and for performing ubiquitination assays.
authors.affiliation: Department of Medical Biosciences, Umeå University, Pathology Building 6M, 2nd Floor, Umeå, 901 85, Sweden.
authors.forename: Shyam Kumar
authors.initials: SK
authors.lastname: Gudey
authors.affiliation: Department of Medical Biosciences, Umeå University, Pathology Building 6M, 2nd Floor, Umeå, 901 85, Sweden. Marene.Landstrom@medbio.umu.se.
authors.forename: Marene
authors.initials: M
authors.lastname: Landström
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006801:Humans; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D051785:Smad Proteins; D016212:Transforming Growth Factor beta
keywords: Non-Smad signaling; TAK1; TGFβ receptor I; TRAF6; Ubiquitination; Ubiquitination assays