title: Frataxin Is Localized to Both the Chloroplast and Mitochondrion and Is Involved in Chloroplast Fe-S Protein Function in Arabidopsis.
abstract: Frataxin plays a key role in eukaryotic cellular iron metabolism, particularly in mitochondrial heme and iron-sulfur (Fe-S) cluster biosynthesis. However, its precise role has yet to be elucidated. In this work, we studied the subcellular localization of Arabidopsis frataxin, AtFH, using confocal microscopy, and found a novel dual localization for this protein. We demonstrate that plant frataxin is targeted to both the mitochondria and the chloroplast, where it may play a role in Fe-S cluster metabolism as suggested by functional studies on nitrite reductase (NIR) and ferredoxin (Fd), two Fe-S containing chloroplast proteins, in AtFH deficient plants. Our results indicate that frataxin deficiency alters the normal functioning of chloroplasts by affecting the levels of Fe, chlorophyll, and the photosynthetic electron transport chain in this organelle.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Valeria R
authors.initials: VR
authors.lastname: Turowski
authors.affiliation: UMR5234 Microbiologie Fondamentale et Pathogénicité, Centre National de la Recherche Scientifique and Université Bordeaux-Segalen, 146 rue Léo Saignat, 33076, Bordeaux cedex, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Aknin
authors.affiliation: Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH) CONICET/UNSAM, Camino de Circunvaación Km 6, 7130, Chascomús, Argentina.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Maliandi
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Celeste
authors.initials: C
authors.lastname: Buchensky
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Leaden
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Diego A
authors.initials: DA
authors.lastname: Peralta
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Busi
authors.affiliation: Centre National de la Recherche Scientifique & UMR 1332 -Biologie du Fruit et Pathologie, Institute National de la Recherche Agronomique (INRA) Bordeaux Aquitaine, 71 avenue Edouard Bourlaux, 33882, Villenave D'Ornon, France.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Araya
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET), Universidad Nacional de Rosario, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Gomez-Casati
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002734:Chlorophyll / Q000032:analysis; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism*; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D033862:Iron-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000502:physiology; D009572:Nitrite Reductases / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D005288:Ferredoxins; D033862:Iron-Binding Proteins; D007506:Iron-Sulfur Proteins; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C518801:frataxin protein, Arabidopsis; D002734:Chlorophyll; C000607454:NIR1 protein, Arabidopsis; D009572:Nitrite Reductases