title: Effects of Various 5,7-Dihydroxyflavone Analogs on Adipogenesis in 3T3-L1 Cells.
abstract: We studied the effects of twelve 5,7-dihydroxyflavone analogs on adipogenesis in 3T3-L1 cells. Among the compounds, luteolin, diosmetin, and chrysoeriol partly inhibited adipogenesis by blocking the accumulation of triacylglycerol in the cells. Conversely, tricetin facilitated triacylglycerol accumulation in the cells. The induction of lipogenesis or lipolysis may depend on the number and bonding position of hydroxyl or methoxy groups on the B ring of 5,7-dihydroxyflavone. The mRNA expression levels of adipogenic and lipogenic genes were suppressed by luteolin treatment in the cells, while the mRNA levels of lipolytic genes were not affected. However, the expression levels of the adipogenic, lipogenic, and lipolytic genes, except for adipocyte protein 2 (aP2), were not affected by the addition of tricetin. Moreover, luteolin suppressed glucose transporter type 4 (GLUT4) gene and protein levels. These results indicate that luteolin decreased triacylglycerol levels in 3T3-L1 cells during adipogenesis through the suppression of adipogenic/lipogenic and GLUT4 genes and GLUT4 protein.
authors.affiliation: College of Science and Technology, Nihon University.
authors.forename: Atsuyoshi
authors.initials: A
authors.lastname: Nishina
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ukiya
authors.forename: Makoto
authors.initials: M
authors.lastname: Fukatsu
authors.forename: Mamoru
authors.initials: M
authors.lastname: Koketsu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ninomiya
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sato
authors.forename: Junpei
authors.initials: J
authors.lastname: Yamamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kobayashi-Hattori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Okubo
authors.forename: Hideyo
authors.initials: H
authors.lastname: Tokuoka
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002867:Chromones / Q000494:pharmacology; D047309:Flavones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D047311:Luteolin / Q000494:pharmacology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002867:Chromones; D047309:Flavones; D005419:Flavonoids; D051275:Glucose Transporter Type 4; D010936:Plant Extracts; D012333:RNA, Messenger; C495693:Slc2a4 protein, mouse; D014280:Triglycerides; C469689:tricetin; D047311:Luteolin; C007054:chrysoeriol; C039602:diosmetin