title: Will the use of double barrier result in sustained release of vancomycin? Optimization of parameters for preparation of a new antibacterial alginate-based modern dressing.
abstract: The aim of this research was to prepare and characterize an alginate-based wound dressing containing vancomycin immobilized at the silica surface. The silica samples functionalized with amine, diol and carboxylic acid groups were loaded with 7.8, 5.7 and 7.1wt.% of the antibiotic respectively. The immobilized drug was encapsulated in alginate or gelatin/alginate gels and the average concentration of vancomycin was about 10mg per g of the dried gel. The effect of functional organic groups at the silica surface on the release rate of the drug was investigated. Only the drug immobilized at Si-amine in alginate matrix was found to demonstrate slower release from the proposed wound dressing. The in vitro release profiles for other silica carriers did not show significant differences in relation to the free loaded drug. The presence of gelatin had a favourable impact on the slowing down of the drug release from the dressing with a double barrier. All the gels studied with vancomycin immobilized at the silica surface demonstrated antimicrobial activity against various bacteria. A reduction of the drug dose to a half had no effect on changing microbiological activity of gels.
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89b, 61-614 Poznań, Poland. Electronic address: asiaw@amu.edu.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Kurczewska
authors.affiliation: Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Science, Święcickiego 4, 60-781 Poznań, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Sawicka
authors.affiliation: Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Science, Święcickiego 4, 60-781 Poznań, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Ratajczak
authors.affiliation: Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Science, Święcickiego 4, 60-781 Poznań, Poland; Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Gajęcka
authors.affiliation: Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89b, 61-614 Poznań, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Schroeder
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D001458:Bandages; D003692:Delayed-Action Preparations; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D012995:Solubility; D014640:Vancomycin / Q000008:administration & dosage* / Q000737:chemistry; D014945:Wound Healing
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D006603:Hexuronic Acids; D014640:Vancomycin; D020723:Glucuronic Acid
keywords: Alginate; Biological activity; Release profile; Silica; Vancomycin; Wound dressing