title: Production, crystallization and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2.
abstract: The molecular details of the formation of the myelin sheath, a multilayered membrane in the nervous system, are to a large extent unknown. P2 is a peripheral membrane protein from peripheral nervous system myelin, which is believed to play a role in this process. X-ray crystallographic studies and complementary experiments have provided information on the structure-function relationships in P2. In this study, a fully deuterated sample of human P2 was produced. Crystals that were large enough for neutron diffraction were grown by a ten-month procedure of feeding, and neutron diffraction data were collected to a resolution of 2.4 Å from a crystal of 0.09 mm(3) in volume. The neutron crystal structure will allow the positions of H atoms in P2 and its fatty-acid ligand to be visualized, as well as shedding light on the fine details of the hydrogen-bonding networks within the P2 ligand-binding cavity.
authors.affiliation: Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, PO Box 5400, 90014 Oulu, Finland.
authors.forename: Saara
authors.initials: S
authors.lastname: Laulumaa
authors.affiliation: Large-Scale Structures Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Blakeley
authors.affiliation: Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, PO Box 5400, 90014 Oulu, Finland.
authors.forename: Arne
authors.initials: A
authors.lastname: Raasakka
authors.affiliation: Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Moulin
authors.affiliation: Life Sciences Group, Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Härtlein
authors.affiliation: Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, PO Box 5400, 90014 Oulu, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Kursula
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D006801:Humans; D008969:Molecular Sequence Data; D018996:Myelin P2 Protein / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D009186:Myelin Sheath / Q000235:genetics* / Q000378:metabolism*; D033363:Neutron Diffraction / Q000379:methods*
chemical_list: D018996:Myelin P2 Protein
keywords: fatty acid-binding protein; myelin; neutron diffraction; perdeuteration; peripheral membrane protein