title: Development of a biomaterial associated with mesenchymal stem cells and keratinocytes for use as a skin substitute.
abstract: OBJECTIVE
  The present study has aimed to produce a cutaneous substitute, bringing together stem cells (mesenchymal stem cells) and keratinocytes, and an electrospun biomaterial.
  
  
  METHODS
  Three groups of scaffolds were studied: group 1, poly-dl-lactic acid (PDLLA); group 2, hydrolyzed PDLLA (PDLLA/NaOH) and group 3, PDLLA/Lam - a PDLLA/NaOH scaffold linked to laminin protein. They were characterized by physicochemical and biological parameters.
  
  
  RESULTS
  As a result, the scaffolds presented well-formed and randomly distributed fibers. Group 3 showed the greatest hydrophilic characteristics. Group 1 showed a greater degradation rate after 14 days. Groups 2 and 3 presented molecular weight of about 40-50 Da. In general, group 3 showed the best results concerning cell adhesion and viability.
  
  
  CONCLUSIONS
  This study associated two revolutionary fields, stem cells and nanotechnology, for use in regenerative medicine.
authors.affiliation: Hematology & Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brazil.|Post-graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Steffens
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares, São Paulo, 05508-000, Brazil.
authors.forename: Monica B
authors.initials: MB
authors.lastname: Mathor
authors.affiliation: Post-graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Bruna T S
authors.initials: BT
authors.lastname: Santi
authors.affiliation: Stem Cell Research Institute (Instituto de Pesquisa com Células-tronco), Porto Alegre, Rio Grande do Sul 90020-010, Brazil.
authors.forename: Dayane P
authors.initials: DP
authors.lastname: Luco
authors.affiliation: Hematology & Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brazil.|Post-graduate Program in Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 90050-170, Brazil.|Stem Cell Research Institute (Instituto de Pesquisa com Células-tronco), Porto Alegre, Rio Grande do Sul 90020-010, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Pranke
mesh_terms: D000293:Adolescent; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D017768:Skin, Artificial; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D007797:Laminin; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: electrospinning; keratinocytes; laminin; scaffolds; skin substitute; stem cells; tissue engineering