title: Is there a correlation between peripheral blood expression of angiogenic transcriptional factors/receptors and colorectal cancer?
abstract: OBJECTIVE
  The aim of this study was to evaluate whether there is a correlation between peripheral blood expression of angiogenic transcriptional factors/receptors and colorectal cancer (CRC).
  
  
  METHODS
  Eighty six blood samples collected from patients with CRC (N=42), adenomas and/or hyperplastic polyps(AP, N=30) and individuals without colon pathology (control group/CTR, N=14) were used for this study. Twelve transcription factors and receptors were assessed by qRT-PCR in a case-control study. The molecules with a minimum of 30% differences in gene expression for CRC and AP compared to CTR were then analyzed separately for each sample. Gene expression was evaluated relatively to the CTR after normalization to the large ribosomal protein PO (RPLPO) housekeeping gene, and the differential expression between studied groups was assessed by ANOVA.
  
  
  RESULTS
  Seven out of 12 genes presented differences in expression between 10-29% in CRC and/or AP compared to CTR. Considering the selection criteria, we further individually evaluated the levels of expression of 5 genes that had a minimum of 30% expression in the case-control study. Our data showed a significant up-regulation of platelet derived growth factor (PDGF) C in the blood of the patients with CRC compared to CTR (p=0.007). Likewise, clusterin (CLU) was significantly up-regulated both in CRC and AP groups compared to healthy subjects (p=0.01). For VEGFR1, PDGFRA and TGFB1 we didn't find significantly differential expression between any of the studied groups, even if increased levels were observed in both CRC and AP vs CTR.
  
  
  CONCLUSIONS
  The results of our study indicated that increased blood level of PDGFC mRNA was associated with the presence of CRC (p=0.007). Additionally, high levels of circulating CLU mRNA were observed in both malignant and benign colorectal pathologies.
authors.affiliation: University of Medicine and Pharmacy "Iuliu Hatieganu" Cluj-Napoca, 1st Medical Clinic, Cluj-Napoca, Romania.
authors.forename: Roberta M
authors.initials: RM
authors.lastname: Manzat-Saplacan
authors.forename: Loredana
authors.initials: L
authors.lastname: Balacescu
authors.forename: Claudia
authors.initials: C
authors.lastname: Gherman
authors.forename: Simona
authors.initials: S
authors.lastname: Visan
authors.forename: Romeo I
authors.initials: RI
authors.lastname: Chira
authors.forename: Adriana
authors.initials: A
authors.lastname: Bintintan
authors.forename: Georgiana
authors.initials: G
authors.lastname: Nagy
authors.forename: Cornelia
authors.initials: C
authors.lastname: Popovici
authors.forename: Simona D
authors.initials: SD
authors.lastname: Valean
authors.forename: Craiu
authors.initials: C
authors.lastname: Anca
authors.forename: Vasile
authors.initials: V
authors.lastname: Bintintan
authors.forename: Razvan
authors.initials: R
authors.lastname: Scurtu
authors.forename: Petru A
authors.initials: PA
authors.lastname: Mircea
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
authors.forename: Tudor E
authors.initials: TE
authors.lastname: Ciuleanu
authors.forename: Ovidiu
authors.initials: O
authors.lastname: Balacescu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D051152:Clusterin / Q000097:blood / Q000235:genetics; D015179:Colorectal Neoplasms / Q000097:blood*; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000097:blood* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D012333:RNA, Messenger / Q000097:blood
chemical_list: C495242:CLU protein, human; D051152:Clusterin; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; C411423:platelet-derived growth factor C