title: Enhancing bio-butanol production from biomass of Chlorella vulgaris JSC-6 with sequential alkali pretreatment and acid hydrolysis.
abstract: This study presents a successful butanol production method using alkali and acid pretreated biomass of Chlorella vulgaris JSC-6. The butanol concentration, yield, and productivity were 13.1g/L, 0.58mol/mol sugar, 0.66g/L/h, respectively. Nearly 2.93L/L of biohydrogen was produced during the acidogenesis phase in ABE fermentation. The hydrogen yield and productivity were 0.39mol/mol sugar and 104.2g/L/h respectively. In addition, the high glucose consumption efficiency (97.5%) suggests that the hydrolysate pretreated with NaOH (1%) followed by H2SO4 (3%) did not contain inhibitors to the fermentation. It was also discovered that an excess amount of nitrogen sources arising from hydrolysis of highly concentrated microalgal biomass negatively affected the butanol production. This work demonstrates the technical feasibility of producing butanol from sustainable third-generation feedstock (i.e., microalgal biomass).
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Wanqian
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chieh-Lun
authors.initials: CL
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Ho
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
mesh_terms: D000143:Acids / Q000737:chemistry; D000468:Alkalies; D018533:Biomass; D000440:Butanols / Q000737:chemistry*; D002241:Carbohydrates / Q000096:biosynthesis*; D048408:Chlorella vulgaris / Q000378:metabolism*; D005285:Fermentation; D005740:Gases / Q000737:chemistry; D006868:Hydrolysis; D058086:Microalgae / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry*
chemical_list: D000143:Acids; D000468:Alkalies; D000440:Butanols; D002241:Carbohydrates; D005740:Gases; D009584:Nitrogen
keywords: ABE fermentation; Butanol; Carbohydrates; Chlorella vulgaris; Microalgae