title: [Diagnostic value of R-banding Technique, Dual-color Fluore- scence In Situ Hybridization and Quantitative Real-time PCR in Acute Promyelocytic Leukemia].
abstract: OBJECTIVE
  To explore the diagnostic value of R-banding technique (RT), dual-color fluorescence in situ hybridization (D-FISH) and quantitative real-time PCR (RT-PCR) for acute promyelocytic leukemia.
  
  
  METHODS
  The cytogenetic characteristics and PML/RARα fusion gene in 340 patients with suspectable APL were analyzed by using 3 detection methods. MICM (morphology, immunology, cytogenetic and molecular biology) was used as diagnostic standard of APL, and the diagnostic value of RT, D-FISH and RT-PCR was evaluated by comparing the detection results of RT, D-FISH and RT-PCR as well as their combination.
  
  
  RESULTS
  For the diagnosis of APL, the sensitivity of RT, D-FISH and RT-PCR was 81.3% (78/96), 95.0% (91/96) and 96.9% (93/96) respectively. RT failed to detect 18 cases, the results of D-FISH showed 5 cases with false positive and 2 cases with false negative, the RT-PCR showed 4 cases with false positive, 3 cases with false negative. The sensitivity and specificity of combined detection of 3 methods were 99.97% and 100% respectively.
  
  
  CONCLUSIONS
  The 3 detection methods alone all have certain defects for diagnosis of APL, but their combined detection is helpful to improve the definitive diagnostic rate and can decrease misdiagnosis rate and missed diagnostic rate.
authors.affiliation: Medical Diagnostic Centre, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang, China.
authors.forename: You-Fan
authors.initials: YF
authors.lastname: Peng
authors.affiliation: Therapeutic Center of Blood Diseases, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Medical Diagnostic Centre, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Medical Diagnostic Centre, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang, China. E-mail: aliyun@163.com.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Zhang
mesh_terms: D002871:Chromosome Banding; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis*; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein