title: Greater amberjack Fsh, Lh, and their receptors: Plasma and mRNA profiles during ovarian development.
abstract: To understand the endocrine regulation of ovarian development in a multiple spawning fish, the relationship between gonadotropins (Gths; follicle-stimulating hormone [Fsh] and luteinizing hormone [Lh]) and their receptors (Gthrs; Fshr and Lhr) were investigated in greater amberjack (Seriola dumerili). cDNAs encoding the Gth subunits (Fshβ, Lhβ, and glycoprotein α [Gpα]) and Gthrs were cloned. The in vitro reporter gene assay using recombinant hormones revealed that greater amberjack Fshr and Lhr responded strongly to their own ligands. Competitive enzyme-linked immunosorbent assays (ELISAs) were developed for measuring greater amberjack Fsh and Lh. Anti-Fsh and anti-Lh antibodies were raised against recombinant chimeric single-chain Gths consisting of greater amberjack Fshβ (or Lhβ) with rabbit GPα. The validation study showed that the ELISAs were precise (intra- and inter-assay coefficient of variation, <10%) and sensitive (detection limit of 0.2ng/ml for Fsh and 0.8ng/ml for Lh) with low cross-reactivity. A good parallelism between the standard curve and serial dilutions of greater amberjack plasma and pituitary extract were obtained. In female greater amberjack, pituitary fshb, ovarian fshr, and plasma E2 gradually increased during ovarian development, and plasma Fsh significantly increased during the post-spawning period. This suggests that Fsh plays a role throughout ovarian development and during the post-spawning period. Pituitary lhb, ovarian lhr, and plasma Lh were high during the spawning period, suggesting that the synthesis and secretion of Lh, and Lhr expression are upregulated to induce final oocyte maturation and ovulation.
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan. Electronic address: nyuujim@fra.affrc.go.jp.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nyuji
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki 519-0423, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Kazeto
authors.affiliation: Institute for East China Sea Research, Nagasaki University, Nagasaki 851-2213, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Izumida
authors.affiliation: Institute for East China Sea Research, Nagasaki University, Nagasaki 851-2213, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tani
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki 519-0423, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.affiliation: Komame Branch, Stock Enhancement Technology Development Center, National Research Institute of Aquaculture, Fisheries Research Agency, Otsuki 788-0315, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Hamada
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama 236-8648, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Mekuchi
authors.affiliation: Seikai National Fisheries Research Institute, Fisheries Research Agency, Nagasaki 851-2231, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Gen
authors.affiliation: Institute for East China Sea Research, Nagasaki University, Nagasaki 851-2213, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Soyano
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki 519-0423, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Okuzawa
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000235:genetics / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology; D010053:Ovary / Q000254:growth & development* / Q000378:metabolism; D010060:Ovulation / Q000378:metabolism; D010473:Perciformes / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D012733:Sex Differentiation / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: ELISA; Gonadotropin; Gonadotropin receptor; Greater amberjack; Multiple spawning fish; Ovarian development