title: The adjuvanticity of ophiopogon polysaccharide liposome against an inactivated porcine parvovirus vaccine in mice.
abstract: In this study, the adjuvant activity of ophiopogon polysaccharide liposome (OPL) was investigated. The effects of OPL on the splenic lymphocyte proliferation of mice were measured in vitro. The results showed that OPL could significantly promote lymphocyte proliferation singly or synergistically with PHA and LPS and that the effect was better than ophiopogon polysaccharide (OP) at most of concentrations. The adjuvant activities of OPL, OP and mineral oil were compared in BALB/c mice inoculated with inactivated PPV in vivo. The results showed that OPL could significantly enhance lymphocyte proliferation, increase the proportion of CD4(+) and CD8(+) T cells, improve the HI antibody titre and specific IgG response, and promote the production of cytokines, and the efficacy of OPL was significantly better than that of OP. In addition, OPL significantly improved the cellular immune response compared with oil adjuvant. These results suggested that OPL possess superior adjuvanticity and that a medium dose had the best efficacy. Therefore, OPL can be used as an effective immune adjuvant for an inactivated PPV vaccine.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Medicinal Engineering Department of Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 450011, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Hou
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: Wuhan Huisheng Biotechnology Group, Wuhan, Hubei 430042, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: sxpxbnl@163.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Song
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D031441:Ophiopogon / Q000737:chemistry*; D010322:Parvoviridae Infections / Q000517:prevention & control*; D028323:Parvovirus, Porcine / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D007074:Immunoglobulin G; D008081:Liposomes; D011134:Polysaccharides; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Adjuvant activity; Ophiopogon polysaccharide liposome; Porcine parvovirus