title: Fission Yeast Cell Cycle Synchronization Methods.
abstract: Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.
authors.affiliation: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain.|Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Tormos-Pérez
authors.affiliation: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain.|Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain.
authors.forename: Livia
authors.initials: L
authors.lastname: Pérez-Hidalgo
authors.affiliation: Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain. smo@usal.es.|Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain. smo@usal.es.
authors.forename: Sergio
authors.initials: S
authors.lastname: Moreno
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects / Q000235:genetics; D009154:Mutation; D012568:Schizosaccharomyces / Q000502:physiology*
chemical_list: D006918:Hydroxyurea
keywords: Block and release; Centrifugal elutriation; Fission yeast; G1-phase; G2-phase; HU; Lactose gradient; Mitosis; S-phase; Schizosaccharomyces pombe; Synchronization; cdc10-129; cdc25-22; nda3-KM311