title: IgG is involved in the migration and invasion of clear cell renal cell carcinoma.
abstract: OBJECTIVE
  To investigate if IgG can be expressed in clear cell renal cell carcinoma (cRCC) , and the expression of IgG is involved in the cancer progression. If IgG expression can serve as a potential target in cancer therapies and be used for judging the prognosis.
  
  
  METHODS
  By immunohistochemistry, we detected IgG in cRCC tissues(75 cRCC tissues and75 adjacent normal kidney tissues). Immunofluorescence and Western blot was used to detect the IgG in cRCC cell lines (786-0, ACHN and CAKI-I). By RT-PCR, the functional transcript of IgG heavy chain was detected. Knockdown of IgG was to analyze the proliferation, migration and invasion ability by CCK8, Transwell and Matrigel and apoptosis in cRCC cell lines.
  
  
  RESULTS
  By immunohistochemistry, we found strong staining of IgG in 66 cases of 75 cRCC tissues and 63 cases of 75 adjacent normal kidney tissues. Immunofluorescence and Western blot was found IgG in cRCC cell lines. Knock-down IgG in cRCC cell lines resulted in significant inhibition of cell proliferation, migration and invasion, and the induction of apoptosis of the 786-0 cells. The immunohistochemistry analysis showed that high IgG expression significantly correlated with the poor differentiation and advanced stage of cRCC.
  
  
  CONCLUSIONS
  IgG was over expressed in cRCC and was involved in the proliferation, migration and invasion of cancer cells. IgG expression may serve as a potential target in cancer therapies and could be used for judging the prognosis.
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: Zhengzuo
authors.initials: Z
authors.lastname: Sheng
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: Caipeng
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Urology, Beijing Jishuitan Hospital, Beijing, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Urology, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: FengZhan
authors.initials: F
authors.lastname: Hu
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: Huaqi
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing, China Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Department of Urology, Second Clinical Medical College of Peking University, Peking University People's Hospital, Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000276:immunology* / Q000556:secondary; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007680:Kidney Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011379:Prognosis; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D007074:Immunoglobulin G
keywords: RENAL CANCER; TUMOUR BIOLOGY; TUMOUR MARKERS