title: Total antioxidant and oxidant status of plasma and renal tissue of cisplatin-induced nephrotoxic rats: protection by floral extracts of Calendula officinalis Linn.
abstract: The present study was aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP) induced nephrotoxic rats and its protection by treatments with floral extracts of Calendula officinalis Linn. Treatment with cDDP elevated (p < 0.05) the levels of blood urea nitrogen, creatinine (CR), TOS, OSI and malondialdehyde (MDA) but lowered (p < 0.05) total plasma proteins, TAS, total thiols (TTH), blood glutathione (GSH) and antioxidant enzymes compared to the control group. Pre- and post-treatments of ethanolic floral extract of C. officinalis along with cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in blood and renal tissue. Ethanolic extract treatments reduced (p < 0.05) MDA level in renal tissue without restoring the erythrocyte MDA level following cDDP treatment. These observations were further supported by the histopathological findings in renal tissue. Observations of the present study have shown that treatments with ethanolic floral extract of C. officinalis protect cDDP induced nephrotoxicity by restoring antioxidant system of the renal tissue.
authors.affiliation: a Division of Veterinary Pharmacology & Toxicology , Division of Pharmacology, FVSC & AH, Sher-e-Kashmir University of Agricultural Sciences & Technology - Jammu , RS Pura, Jammu, Jammu & Kashmir , India .
authors.forename: Pawan Kumar
authors.initials: PK
authors.lastname: Verma
authors.affiliation: b Division of Veterinary Pharmacology and Toxicology , Faculty of Veterinary Sciences and Animal Husbandry , RS Pura, Jammu, Jammu & Kashmir , India .
authors.forename: Rajinder
authors.initials: R
authors.lastname: Raina
authors.affiliation: c Division of Veterinary Pharmacology and Toxicology , Faculty of Veterinary Sciences and Animal Husbandry , RS Pura, Jammu, Jammu & Kashmir , India .
authors.forename: Mudasir
authors.initials: M
authors.lastname: Sultana
authors.affiliation: d Division of Veterinary Public Health and Epidemiology , Faculty of Veterinary Sciences and Animal Husbandry , RS Pura, Jammu, Jammu & Kashmir , India , and.
authors.forename: Maninder
authors.initials: M
authors.lastname: Singh
authors.affiliation: e Division of Pathology , Indian Veterinary Research Institute , Izatnagar, Bareilly, Uttar Pradesh , India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000378:metabolism; D020838:Calendula; D002945:Cisplatin / Q000009:adverse effects*; D004353:Drug Evaluation, Preclinical; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D010936:Plant Extracts; D002945:Cisplatin
keywords: Calendula officinalis; cisplatin; nephrotoxicity; oxidative stress index; total antioxidant status