title: Anti-tumor and immunomodulatory activity of selenium (Se)-polysaccharide from Se-enriched Grifola frondosa.
abstract: A polysaccharide termed Se-GP11 was extracted and purified from Se-enriched Grifola frondosa in our previous study. This study investigated the characterization, anti-tumor and immunomodulatory activity of Se-GP11. The results showed that Se-GP11 was composed of mannose, glucose and galactose with a molar ratio of 1:4.91:2.41. The weight-average molecular weight (Mw) and weight-average mean square radius (Rw) of Se-GP11 in 0.1M sodium chloride solution were 3.3×10(4)Da and 32.8 nm. Se-GP11 existed as a globular conformation with random coil structure. Se-GP11 had no anti-tumor activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps tumor in vivo. Se-GP11 increased the relatively thymus and spleen weights as well as serum necrosis factor-alpha (TNF-α) and interleukin-2 (IL-2) levels. In addition, Se-GP11 promoted the phagocytosis and NO production of RAW264.7 as compared with that of the normal control group. The results revealed that the Se-GP11 may exhibit the anti-tumor through improving immunologic function of the tumor bearing mice.
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu, Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Mao
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ren
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Wu
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Dun
authors.initials: D
authors.lastname: Jin
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Zhejiang Fangge Pharmaceutical Co., Ltd., Xingguang Rd., 323800 Qingyuan, Zhejiang, China.
authors.forename: Cai-Quan
authors.initials: CQ
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Deng-Hong
authors.initials: DH
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Jia
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
authors.forename: Yan-Peng
authors.initials: YP
authors.lastname: Bai
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: yangliuqing@ujs.edu.cn.
authors.forename: Liu-Qing
authors.initials: LQ
authors.lastname: Yang
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, 301 Xuefu, Rd., 212013 Zhenjiang, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Wu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D015415:Biomarkers; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D048691:Grifola / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D012643:Selenium / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D012643:Selenium
keywords: Anti-tumor; Characterization; Immunomodulatory; Polysaccharide; Se-enriched Grifola frondosa