title: Practical survey on antibiotic-resistant bacterial communities in livestock manure and manure-amended soil.
abstract: Through livestock manure fertilization, antibiotics, antibiotic-resistant bacteria and genes are transferred to agricultural soils, resulting in a high prevalence of antibiotic-resistant bacteria in the soil. It is not clear, however, whether a correlation exists between resistant bacterial populations in manure and manure-amended soil. In this work, we demonstrate that the prevalence of cephalexin-, amoxicillin-, kanamycin- and gentamicin-resistant bacteria as well as bacteria simultaneously resistant to all four antibiotics was much higher in manure-amended soils than in manure-free soil. 454-pyrosequencing indicated that the ARB and multiple antibiotic-resistant bacteria (MARB) in swine or chicken manure and manure-amended soil were mainly distributed among Sphingobacterium, Myroides, Enterococcus, Comamonas and unclassified Flavobacteriaceae. The genus Sphingobacterium was highly prevalent among ARB from swine manure and manure-amended soil, and was also the most dominant genus among MARB from chicken manure and manure-amended soil. Other dominant genera among ARB or MARB populations in manure samples, including Myroides, Enterococcus and Comamonas, could not be detected or were detected at very low relative abundance in manure-amended soil. The present study suggests the possibility of transfer of ARBs from livestock manures to soils and persistence of ARB in these environments.
authors.affiliation: a College of Life Sciences, Henan Normal University , Xinxiang , China.|b Key Laboratory for Microorganisms and Functional Molecules (Henan Normal University), University of Henan Province , Xinxiang , China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Yang
authors.affiliation: a College of Life Sciences, Henan Normal University , Xinxiang , China.|b Key Laboratory for Microorganisms and Functional Molecules (Henan Normal University), University of Henan Province , Xinxiang , China.
authors.forename: Ruifei
authors.initials: R
authors.lastname: Wang
authors.affiliation: a College of Life Sciences, Henan Normal University , Xinxiang , China.
authors.forename: Siwei
authors.initials: S
authors.lastname: Ren
authors.affiliation: c Department of Plant & Soil Sciences , College of Agriculture, Food and Environment, University of Kentucky , Lexington , Kentucky , USA.
authors.forename: Marton
authors.initials: M
authors.lastname: Szoboszlay
authors.affiliation: c Department of Plant & Soil Sciences , College of Agriculture, Food and Environment, University of Kentucky , Lexington , Kentucky , USA.
authors.forename: Luke A
authors.initials: LA
authors.lastname: Moe
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology; D002681:China; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial; D008372:Manure / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology; D034421:Sus scrofa / Q000382:microbiology
chemical_list: D008372:Manure
keywords: 454-pyrosequencing; antibiotic resistance; antibiotic resistant bacteria; bacterial community; livestock manure; manure-amended soil