title: A Review of Fluorescent Proteins for Use in Yeast.
abstract: The field of fluorescent proteins (FPs) is constantly developing. The use of FPs changed the field of life sciences completely, starting a new era of direct observation and quantification of cellular processes. The broad spectrum of FPs (see Fig. 1) with a wide range of characteristics allows their use in many different experiments. This review discusses the use of FPs for imaging in budding yeast (Saccharomyces cerevisiae) and fission yeast Schizosaccharomyces pombe). The information included in this review is relevant for both species unless stated otherwise.
authors.affiliation: Department of Developmental and Cell Biology, Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.|Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA.
authors.forename: Maja
authors.initials: M
authors.lastname: Bialecka-Fornal
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, San Francisco, CA, 94158, USA.
authors.forename: Tatyana
authors.initials: T
authors.lastname: Makushok
authors.affiliation: Department of Developmental and Cell Biology, Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA. susanner@uci.edu.|Center for Complex Biological Systems, University of California, Irvine, CA, 92697, USA. susanner@uci.edu.
authors.forename: Susanne M
authors.initials: SM
authors.lastname: Rafelski
mesh_terms: D016208:Databases, Factual; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods; D064878:Web Browser; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
chemical_list: D008164:Luminescent Proteins
keywords: Fluorescence microscopy; Fluorescent protein s; Protein tagg ing; Saccharomyces cerevisiae; Schizosaccharomyces pombe; Yeast