title: Iloprost and vitamin C attenuates acute myocardial injury induced by suprarenal aortic ischemia-reperfusion in rabbits.
abstract: OBJECTIVE
  The aim of this study was to evaluate antioxidant and cytoprotective effects of iloprost and Vitamin C in a distant organ after abdominal aorta ischemia-reperfusion injury.
  
  
  METHODS
  Twenty-eight New Zealand rabbits weighing 2,400-2,800 g were used for this study. The rabbits were divided into four equal groups. These groups are control group, sham group, iloprost group, and iloprost+vitamin C group. Suprarenal aorta was occluded with a vascular clamp. Following 30 minutes of ischemia, the vascular clamp was removed. Rabbits in group 3 received 10 ng/kg/min iloprost and those in group 4 received 10 ng/kg/min iloprost and 10 mg/kg vitamin C. At the end of the reperfusion period, the rabbits were sacrificed by a high intraperitoneal dose of xylazine+ketamine injection. Myocardial tissue samples were taken for electron microscopic analysis. We evaluated SOD, MDA and catalase in myocardial tissue samples.
  
  
  RESULTS
  Iloprost and iloprost+vitamin C groups significantly reduced the oxidative stress markers in tissue samples (p<0.05) and significantly decreased the myofibrillar injury and mitochondrial morphology changes in the myocardial tissue as shown with electron microscopy (p<0.05). Myocardial edema was significantly alleviated by iloprost and iloprost+vitamin C administration (p<0.05).
  
  
  CONCLUSIONS
  This study clearly showed that myocardial injury and edema occurred after ischemia-reperfusion of abdominal aorta and that groups administered with iloprost and iloprost+vitamin C showed an attenuation of ischemia-reperfusion injury in distant organs (Tab. 3, Fig. 4, Ref. 30).
authors.forename: E
authors.initials: E
authors.lastname: Iriz
authors.forename: A
authors.initials: A
authors.lastname: Iriz
authors.forename: G
authors.initials: G
authors.lastname: Take
authors.forename: H
authors.initials: H
authors.lastname: Ozgul
authors.forename: L
authors.initials: L
authors.lastname: Oktar
authors.forename: H
authors.initials: H
authors.lastname: Demirtas
authors.forename: F
authors.initials: F
authors.lastname: Helvacioglu
authors.forename: M
authors.initials: M
authors.lastname: Arslan
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects; D001205:Ascorbic Acid / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D016285:Iloprost / Q000494:pharmacology*; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011817:Rabbits; D015427:Reperfusion Injury / Q000503:physiopathology; D014815:Vitamins / Q000494:pharmacology
chemical_list: D010975:Platelet Aggregation Inhibitors; D014815:Vitamins; D016285:Iloprost; D001205:Ascorbic Acid
keywords: electron microscopy.; iloprost; ischemia-reperfusion; myocardium; remote