title: Access to side-chain carbon information in deuterated solids under fast MAS through non-rotor-synchronized mixing.
abstract: We demonstrate the accessibility of aliphatic (13)C side chain chemical shift sets for solid-state NMR despite perdeuteration and fast MAS using isotropic, non-rotor-synchronized (13)C-(13)C mixing. Combined with amide proton detection, we unambiguously and sensitively detect whole side chain to backbone correlations for two proteins using around 1 mg of sample.
authors.affiliation: Max-Planck Institute for Biophysical Chemistry, Department NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany. rali@nmr.mpibpc.mpg.de.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kulminskaya
authors.forename: Suresh Kumar
authors.initials: SK
authors.lastname: Vasa
authors.forename: Karin
authors.initials: K
authors.lastname: Giller
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.forename: Ann
authors.initials: A
authors.lastname: Kwan
authors.forename: Margaret
authors.initials: M
authors.lastname: Sunde
authors.forename: Rasmus
authors.initials: R
authors.lastname: Linser