title: MicroRNA-214 and MicroRNA-126 Are Potential Biomarkers for Malignant Endothelial Proliferative Diseases.
abstract: Malignant endothelial proliferative diseases including human angiosarcoma (AS) and canine hemangiosarcoma (HSA) are serious diseases with a grave prognosis. Establishing liquid biopsy-based biomarkers for screening has definite clinical utility; however, plasma miRNAs up- or down-regulated in these sarcomas have been unclear. For identifying possible diagnostic plasma miRNAs for these sarcomas, we investigated whether plasma miR-214 and miR-126, which miRNAs play important roles in angiogenesis and tumorigenesis, were elevated in malignant endothelial proliferative diseases. For this investigation, human angiosarcoma and canine hemangiosarcoma cell lines and clinical plasma samples of canine hemangiosarcoma were examined by performing miRNA qRT-PCR. We report here that human angiosarcoma and canine hemangiosarcoma cell lines over-secreted miR-214 and miR-126 via microvesicles; in addition, their levels in the plasma samples from canines with hemangiosarcoma were increased. Moreover, the surgical resection of primary tumors decreased the levels of plasma miR-214 and miR-126. Our findings suggest that these malignant endothelial proliferative diseases over-secreted miR-214 and miR-126, thus suggesting that these miRNAs have potential as diagnostic biomarkers for malignant endothelial proliferative diseases in canine and possible in human angiosarcoma.
authors.affiliation: Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. kheishima@live.jp.|United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan. kheishima@live.jp.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Heishima
authors.affiliation: Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. tmori@gifu-u.ac.jp.|United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan. tmori@gifu-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.affiliation: Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. lilymusashisoleil@yahoo.co.jp.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Ichikawa
authors.affiliation: United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan. shiroki@gifu-u.ac.jp.|Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. shiroki@gifu-u.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sakai
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 501-1193, Japan. t3125010@edu.gifu-u.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kuranaga
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. anakaga@mail.ecc.u-tokyo.ac.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. yui.tanaka.810@gmail.com.
authors.forename: Yuiko
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan. okamura@iwate-u.ac.jp.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Okamura
authors.affiliation: Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan. mikio@med.kitasato-u.ac.jp.
authors.forename: Mikio
authors.initials: M
authors.lastname: Masuzawa
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 501-1193, Japan. t3125016@edu.gifu-u.ac.jp.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Sugito
authors.affiliation: Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. muramami@gifu-u.ac.jp.
authors.forename: Mami
authors.initials: M
authors.lastname: Murakami
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 501-1193, Japan. namiyamada80@gmail.com.
authors.forename: Nami
authors.initials: N
authors.lastname: Yamada
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu 501-1193, Japan. yakao@gifu-u.ac.jp.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
authors.affiliation: Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. kmaruo@gifu-u.ac.jp.|United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan. kmaruo@gifu-u.ac.jp.
authors.forename: Kohji
authors.initials: K
authors.lastname: Maruo
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D045744:Cell Line, Tumor; D004285:Dogs; D006394:Hemangiosarcoma / Q000097:blood* / Q000662:veterinary; D006801:Humans; D035683:MicroRNAs / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: angiosarcoma; biomarker; endothelial cell; hemangiosarcoma; microRNA