title: Rapid detection of fumonisin B1 using a colloidal gold immunoassay strip test in corn samples.
abstract: Fumonisin B1 (FB1) is the most common and highest toxic of fumonisins species, exists frequently in corn and corn-based foods, leading to several animal and human diseases. Furthermore, FB1 was reported that it was associated with the human esophageal cancer. In view of the harmful of FB1, it is urgent to develop a feasible and accuracy method for rapid detection of FB1. In this study, a competitive immunoassay for FB1 detection was developed based on colloidal gold-antibody conjugate. The FB1-keyhole limpet hemoeyanin (FB1-KLH) conjugate was embedded in the test line, and goat anti-mouse IgG antibody embedded in the control line. The color density of the test line correlated with the concentration of FB1 in the range from 2.5 to 10 ng/mL, and the visual limit detection of test for FB1 was 2.5 ng/mL. The results indicated that the test strip is specific for FB1, and no cross-reactivity to other toxins. The quantitative detection for FB1 was simple, only needing one step without complicated assay performance and expensive equipment, and the total time of visual evaluation was less than 5 min. Hence, the developed colloidal gold-antibody assay can be used as a feasible method for FB1 rapid and quantitative detection in corn samples.
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Ling
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Rongzhi
authors.initials: R
authors.lastname: Wang
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wen
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Qing-Ai
authors.initials: QA
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: xzhzhenhong@163.com.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Pathogenic Fungi and Mycotxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: wshyyl@sina.com.
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
mesh_terms: D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005670:Fusarium / Q000378:metabolism*; D017979:Gold Colloid / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D003313:Zea mays / Q000382:microbiology*
chemical_list: D037341:Fumonisins; D017979:Gold Colloid; C056933:fumonisin B1
keywords: Colloidal gold immunoassay; Fumonisin B(1); Hybridoma; Quantitative detection