title: Evaluation of T, B and natural killer lymphocyte in the cervical stroma of HIV-positive and negative patients with cervical intraepithelial neoplasia.
abstract: Cervical intraepithelial neoplasias (CIN) are closely associated with oncogenic subtypes of the human papillomavirus (HPV). In the presence of this virus, it is known that the activation or suppression of immune system is the key to the development, progression and/or regression of cervical lesions. Therefore, the objective of this study is to compare the local immune response among HIV-seropositive and seronegative patients with cervical intraepithelial neoplasia regarding the expression of T lymphocytes (CD3+, CD4+ and CD8+), B lymphocytes (CD20+) and natural killers cells (CD56+) in the cervical stroma. A cross-sectional study of paraffin blocks containing cervical tissue after conization by the Loop Electrosurgical Excision Procedure (LEEP) from 47 HIV-seropositive and 38 seronegative patients with CIN. Cervical stroma immunohistochemistry was performed in the CIN area. The Fisher's exact test was used for the statistical analysis. When HIV-seropositive and seronegative women were compared, the seropositive women had a higher count of CD8+ T lymphocytes (52.1% versus 28.9%, P<0.04). Considering CIN degree (CIN 1 and CIN 2/3), the HIV-seronegative patients with CIN 1 had a low count of CD20+B-lymphocytes (7.1%) in comparison with CIN 1 HIV seropositive and with CIN 2/3 HIV-seronegative patients, respectively 50% (P<0.018) and 54.5% (P<0.0048). The HIV infection and degree of CIN influenced the cytotoxic lymphocytes inducing an increase in the number of cells high count of CD20+ lymphocytes with CIN 1.
authors.affiliation: Holy House of Mercy Hospital of Belo Horizonte, Santa Casa de Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Adriana A S
authors.initials: AA
authors.lastname: Lucena
authors.affiliation: Holy House of Mercy Hospital of Belo Horizonte, Santa Casa de Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Mírian Viviane M B
authors.initials: MV
authors.lastname: Guimarães
authors.affiliation: Research Institute of Oncology, Discipline of Gynecology and Obstetrics, Federal University of Triângulo Mineiro (Universidade Federal do Triângulo Mineiro), Brazil. Electronic address: michelinimuno@icbn.uftm.edu.br.
authors.forename: Márcia A
authors.initials: MA
authors.lastname: Michelin
authors.affiliation: Minas Gerais School of Medical Sciences, (Faculdade de Ciências Médicas de Minas Gerais), Brazil.
authors.forename: Cláudia T C
authors.initials: CT
authors.lastname: Lodi
authors.affiliation: Holy House of Mercy Hospital of Belo Horizonte, Santa Casa de Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Maria Inês M
authors.initials: MI
authors.lastname: Lima
authors.affiliation: Research Institute of Oncology, Discipline of Gynecology and Obstetrics, Federal University of Triângulo Mineiro (Universidade Federal do Triângulo Mineiro), Brazil.
authors.forename: Eddie Fernando Candido
authors.initials: EF
authors.lastname: Murta
authors.affiliation: School of Medicine - Federal University of Minas Gerais (Faculdade de Medicina da Universidade Federal de Minas Gerais), Brazil.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Melo
mesh_terms: D000328:Adult; D018951:Antigens, CD20 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D003430:Cross-Sectional Studies; D003602:Cytotoxicity, Immunologic; D005260:Female; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D006679:HIV Seropositivity; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D018655:Lymphocyte Count; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000175:diagnosis* / Q000276:immunology
chemical_list: D018951:Antigens, CD20
keywords: B-lymphocytes; Cervical intraepithelial neoplasia; Human immunodeficiency virus; Natural killer cells; T lymphocytes