title: Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics.
abstract: Extracellular vesicles (EVs) are cell-derived nanovesicles, present in almost all types of body fluids, which play an important role in intercellular communication and are involved in the transport of biological signals for regulating diverse cellular functions. Due to the increasing clinical interest in the role of EVs in tumor promotion, various techniques for their isolation, detection, and characterization are being developed. In this review, we present an overview of the current EV isolation and characterization methods in addition to their applications and limitations. Furthermore, EVs as the potential emerging biomarkers in cancer management and their clinical implementation are briefly discussed.
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 689-798, Republic of Korea. ykcho@unist.ac.kr.
authors.forename: Vijaya
authors.initials: V
authors.lastname: Sunkara
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 689-798, Republic of Korea. ykcho@unist.ac.kr.
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Woo
authors.affiliation: Center for Soft and Living Matter, Institute for Basic Science (IBS), UNIST-gil 50, Ulsan 689-798, Republic of Korea. ykcho@unist.ac.kr and Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), UNIST-gil 50, Ulsan 689-798, Republic of Korea. ykcho@unist.ac.kr.
authors.forename: Yoon-Kyoung
authors.initials: YK
authors.lastname: Cho
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D000067128:Extracellular Vesicles / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D011379:Prognosis