title: Osteogenic potential and synergistic effects of growth factors delivered from a bionic composite system.
abstract: Previous research has raised substantial controversy over the synergistic effects of exogenous growth factors, BMP-2 and bFGF, when used together for the treatment of bony defects. Thus, this study evaluated the effects of BMP-2 and bFGF at specified dose ratio composited with n-HA/PU40, a porous scaffold material, for repairing femoral defect in rats. Four weeks after implantation of this composite system, tissue specimens were collected for histological, immunohistochemical examinations, and µ-CT scanning. The results showed that the group DUAL/BMSCs with both the factors had better effect on repairing bone defects than the other four groups in terms of new bone formation and bone-scaffold bonding, suggesting crosstalk between these growth factors during early bone regeneration. This work demonstrates that provided that there is effective contact between cells and active proteins in the defect area, the controlled release of bFGF and BMP-2 have positive synergistic effects on early bone formation in the defect area. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 659-668, 2016.
authors.affiliation: State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford School of Medicine, Stanford University, Stanford, California, 94305.
authors.forename: Alan W
authors.initials: AW
authors.lastname: Gomez
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.affiliation: State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, 610064, China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, 610041, China.
authors.forename: Jihua
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001701:Bionics / Q000379:methods*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology; D007267:Injections; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D008863:Microspheres; D010012:Osteogenesis / Q000187:drug effects*; D020442:Poloxamer / Q000737:chemistry; D016062:Porosity; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D034421:Sus scrofa; D013696:Temperature; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: D055396:Bone Morphogenetic Protein 2; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D016222:Fibroblast Growth Factor 2; D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D017886:Durapatite
keywords: BMP-2; bFGF; gelatin microspheres; porous scaffold; synergistic effects