title: Designing repeat proteins for biosensors and medical imaging.
abstract: Advances in protein engineering tools, both computational and experimental, has afforded many new protein structures and functions. Here, we present a snapshot of repeat-protein engineering efforts towards new, versatile, alternative binding scaffolds for use in analytical sensors and as imaging agents. Analytical assays, sensors and imaging agents based on the direct binding of analyte are increasingly important for research and diagnostics in medicine, food safety, and national security.
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg, VA 24060, U.S.A.
authors.forename: Rachael N
authors.initials: RN
authors.lastname: Parker
authors.affiliation: Department of Chemistry, Virginia Tech, Blacksburg, VA 24060, U.S.A. tijana.grove@vt.edu.
authors.forename: Tijana Z
authors.initials: TZ
authors.lastname: Grove
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000639:trends; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D057054:Molecular Imaging / Q000639:trends; D011487:Protein Conformation; D015202:Protein Engineering; D020449:Repetitive Sequences, Amino Acid
chemical_list: D008024:Ligands
keywords: alternative protein scaffold; biosensor; imaging agent; molecular recognition; protein design; repeat proteins