title: Two-dimensional single-cell patterning with one cell per well driven by surface acoustic waves.
abstract: In single-cell analysis, cellular activity and parameters are assayed on an individual, rather than population-average basis. Essential to observing the activity of these cells over time is the ability to trap, pattern and retain them, for which previous single-cell-patterning work has principally made use of mechanical methods. While successful as a long-term cell-patterning strategy, these devices remain essentially single use. Here we introduce a new method for the patterning of multiple spatially separated single particles and cells using high-frequency acoustic fields with one cell per acoustic well. We characterize and demonstrate patterning for both a range of particle sizes and the capture and patterning of cells, including human lymphocytes and red blood cells infected by the malarial parasite Plasmodium falciparum. This ability is made possible by a hitherto unexplored regime where the acoustic wavelength is on the same order as the cell dimensions.
authors.affiliation: Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Collins
authors.affiliation: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Morahan
authors.affiliation: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Jose
authors.initials: J
authors.lastname: Garcia-Bustos
authors.affiliation: Department of Microbiology, Infection and Immunity Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Christian
authors.initials: C
authors.lastname: Doerig
authors.affiliation: Department of Immunology, Alfred Hospital Precinct, Monash University, Melbourne, Victoria 3004, Australia.|Therapeutics and Regenerative Division, Monash Institute of Medical Engineering, MIME, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Plebanski
authors.affiliation: Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Adrian
authors.initials: A
authors.lastname: Neild
mesh_terms: D001696:Biomechanical Phenomena; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D013016:Sound