title: Stimulation of Cardiomyocyte Regeneratory Reactions under Conditions of Cytopathic Hypercholesterolemia.
abstract: The regeneratory reactions of cardiomyocytes in the heart under conditions of cytopathic exposure to hypercholesterolemia and during verapamil treatment were studied by immunohistochemical detection of proliferation marker Ki-67 and evaluation of cardiomyocyte count. A 30-day exposure of rats to atherogenic diet led to an increase of Ki-67+ cardiomyocytes by 14.5-16.7 times (p<0.05). The Ki-67 label index in cardiomyocytes remained higher than normally (8-9-fold; p<0.05) after 64 days. It remained elevated (8-11-fold; p<0.05) after verapamil treatment. Evaluation of cardiomyocyte count and of their nuclear status detected various regeneratory strategies: increase of the total cardiomyocyte count, with increase of the percentage of mononuclear cardiomyocytes (particularly in response to verapamil in group 1 rats); decrease of total cardiomyocyte count with increase of the percentage of multinuclear cardiomyocytes (particularly in group 2 rats in response to verapamil).
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia. pathol@inbox.ru.
authors.forename: E L
authors.initials: EL
authors.lastname: Lushnikova
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Yuzhik
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Klinnikova
authors.affiliation: Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia.
authors.forename: L M
authors.initials: LM
authors.lastname: Nepomnyashchikh
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002908:Chronic Disease; D006937:Hypercholesterolemia / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology
chemical_list: D014700:Verapamil
keywords: Ki-67; cardiomyocytes; hypercholesterolemia; regeneration; verapamil