title: Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake.
abstract: Nanoparticles (NPs) in biological fluids immediately interact with proteins forming a biomolecular corona (PC) that imparts their biological identity. While several studies on the formation of the PC in human plasma have been reported, the PC of orally administrated NPs has been less investigated, mostly in the presence of a food matrix. In fact, food matrixes when digested are subject of several dynamic changes that will certainly affect the PC formed on the NPs. The lack of studies on this topic is clearly related to the difficulty in isolating representative PC NPs from such a complex environment. In this work magnetite NPs were added to in vitro simulated digestion simultaneously with bread and PC NPs were isolated after gastric and duodenal phases by sucrose gradient ultracentrifugation (UC). The PC NPs were characterized in terms of size and protein composition. Translocation studies were then performed on Caco-2 monolayers in a serum free environment and cell morphology was characterized by confocal microscopy. PC NPs isolated from gastric and duodenal phases were different in size, surface charge and protein corona composition. NP cellular uptake was enhanced by the digestive PC inducing morphology changes in the cell monolayer. Overall, in this work we were able to isolate PC NPs from digested fluids in the presence of a food matrix and study their biological response on Caco-2 cells.
authors.affiliation: School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, United Kingdom; Institute of Food Research, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Desirè
authors.initials: D
authors.lastname: Di Silvio
authors.affiliation: Institute of Food Research, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Neil
authors.initials: N
authors.lastname: Rigby
authors.affiliation: Institute of Food Research, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Balazs
authors.initials: B
authors.lastname: Bajka
authors.affiliation: Institute of Food Research, Norwich Research Park, Norwich, United Kingdom.
authors.forename: Alan
authors.initials: A
authors.lastname: Mackie
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Milan, Italy. Electronic address: francesca.baldelli@polimi.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Baldelli Bombelli
mesh_terms: D040761:Biomimetic Materials / Q000378:metabolism; D001826:Body Fluids / Q000378:metabolism; D001939:Bread; D004063:Digestion; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D000066970:Protein Corona / Q000737:chemistry* / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D058185:Magnetite Nanoparticles; D000066970:Protein Corona
keywords: Bread; Caco-2; Cell uptake; Gastrointestinal digestion; Nanoparticle; Protein corona