title: Development of microsatellite markers for Suriana maritima (Surianaceae) using next-generation sequencing technology.
abstract: Our objective was to develop microsatellite markers for use in assessing genetic variation in the small shrub or tree species Suriana maritima (Surianaceae). In China, this species is found only as a few fragmented populations and individuals on the Paracel Islands. Using next-generation genome sequencing methodology, we developed 17 novel microsatellite markers for S. maritima. Fifty-four individuals from six populations of S. maritima were examined for polymorphisms; only one allele was detected for each of the markers. Microsatellite loci developed indicate a complete absence of genetic diversity for S. maritima on the Paracel Islands in China. These markers will be useful for examining genetic variation among S. maritima populations in other areas of the world.
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: W S
authors.initials: WS
authors.lastname: Chen
authors.affiliation: Zhongkai University of Agriculture and Engineering, Guangzhou, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhao
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China jiansg@scbg.ac.cn.
authors.forename: S G
authors.initials: SG
authors.lastname: Jian
authors.affiliation: South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Z F
authors.initials: ZF
authors.lastname: Wang
mesh_terms: D000483:Alleles; D015415:Biomarkers / Q000032:analysis; D002681:China; D014644:Genetic Variation; D018745:Genome, Plant; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats; D018515:Plant Leaves / Q000235:genetics
chemical_list: D015415:Biomarkers