title: Simultaneous determination of 3-O-acetyloleanolic acid and oleanolic acid in rat plasma using liquid chromatography coupled to tandem mass spectrometry.
abstract: 3-O-Acetyloleanolic acid (OAA) is a triterpenoid compound, and exerts an apoptosis in cancer cell lines, an inhibition of both atopic and allergic contact dermatitis in murine model, and a suppression of inflammatory bone loss in mice. OAA can be converted into oleanolic acid (OA) by hydrolysis in vivo, and OA exhibits several pharmacological effects as well. A liquid chromatographic method using tandem mass spectrometry (MS/MS) was developed for the simultaneous determination of OAA and OA in rat plasma. After liquid-liquid extraction with ethylacetate, both substances were chromatographed on a reversed phase column with a mobile phase of 0.1% formic acid aqueous solution and acetonitrile (1:9, v/v). The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This analytical method was successfully applied to monitor plasma concentrations of both substances over time following an intravenous administration of OAA in rats.
authors.affiliation: Center for Metareceptome Research, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Kim
authors.affiliation: Center for Metareceptome Research, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea.
authors.forename: Keumhan
authors.initials: K
authors.lastname: Noh
authors.affiliation: Center for Metareceptome Research, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Kyoungbuk 712-702, South Korea.
authors.forename: Beomsoo
authors.initials: B
authors.lastname: Shin
authors.affiliation: Bio-Material Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, South Korea.
authors.forename: Joo Tae
authors.initials: JT
authors.lastname: Hwang
authors.affiliation: Bio-Material Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, South Korea.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Bio-Material Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, South Korea. Electronic address: rho-m@kribb.re.kr.
authors.forename: Mun-Chul
authors.initials: MC
authors.lastname: Rho
authors.affiliation: Center for Metareceptome Research, College of Pharmacy, Chung-Ang University, Seoul 156-756, South Korea. Electronic address: wkang@cau.ac.kr.
authors.forename: Wonku
authors.initials: W
authors.lastname: Kang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D009828:Oleanolic Acid / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000097:blood*
chemical_list: D014315:Triterpenes; C052658:oleanolic acid 3-acetate; D009828:Oleanolic Acid
keywords: 3-O-Acetyloleanolic acid; LC–MS/MS; Oleanolic acid; Rat plasma