title: Preliminary evaluation of the potential of 99mTc carbonyl-DTPA-Rituximab as a tracer for sentinel lymph node detection.
abstract: Preliminary work with (99m)Tc carbonyl-DTPA-Rituximab was attempted to test its feasibility as a sentinel lymph node (SLN) tracer for patients with breast cancer. (99m)Tc labeling of DTPA-Rituximab conjugate was carried out via (99m)Tc carbonyl synthon which exhibited >95% radiochemical purity and good in vitro stability. In vitro studies of (99m)Tc carbonyl-DTPA-Rituximab in normal and malignant B cells showed higher binding in malignant cells. In vivo distribution of (99m)Tc carbonyl-DTPA-Rituximab in Wistar rat footpad model indicated good retention by B-cells present in the sentinel lymph node.
authors.affiliation: Isotope Production and Applications Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Electronic address: kmythili@barc.gov.in.
authors.forename: Mythili
authors.initials: M
authors.lastname: Kameswaran
authors.affiliation: Isotope Production and Applications Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Subramanian
authors.affiliation: Isotope Production and Applications Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: Usha
authors.initials: U
authors.lastname: Pandey
authors.affiliation: Isotope Production and Applications Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India.
authors.forename: Grace
authors.initials: G
authors.lastname: Samuel
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000493:pharmacokinetics; D001402:B-Lymphocytes / Q000000981:diagnostic imaging; D001943:Breast Neoplasms / Q000000981:diagnostic imaging; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging*; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D000069283:Rituximab; D021701:Sentinel Lymph Node Biopsy
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C000592761:technetium 99m carbonyl DTPA-rituximab; D000069283:Rituximab
keywords: Breast lymphoma; Rituximab; Sentinel lymph node; Technetium radiopharmaceuticals