title: Plasmacytoid dendritic cells are dispensable for noninfectious intestinal IgA responses in vivo.
abstract: Intestinal DCs orchestrate gut immune homeostasis by dampening proinflammatory T-cell responses and inducing anti-inflammatory IgA responses. Although no specific DC subset has been strictly assigned so far to govern IgA response, some candidate subsets emerge. In particular, plasmacytoid DCs (pDCs), which notoriously promote anti-viral immunity and T-cell tolerance to innocuous antigens (Ags), contribute to IgA induction in response to intestinal viral infection and promote T-cell-independent IgA responses in vitro. Here, using two transgenic mouse models, we show that neither short-term nor long-term pDC depletion alters IgA class switch recombination in Peyer's patches and frequency of IgA plasma cells in intestinal mucosa at steady state, even in the absence of T-cell help. In addition, pDCs are dispensable for induction of intestinal IgA plasma cells in response to oral immunization with T-cell-dependent or T-cell-independent Ags, and are not required for proliferation and IgA switch of Ag-specific B cells in GALT. These results show that pDCs are dispensable for noninfectious IgA responses, and suggest that various DC subsets may play redundant roles in the control of intestinal IgA responses.
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Moro-Sibilot
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: This
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Blanc
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.|Cancerology Research Center of Lyon, Inserm U1052 - CNRS UMR5286, Lyon, France.
authors.forename: Amelien
authors.initials: A
authors.lastname: Sanlaville
authors.affiliation: Departments of Pathology and Medicine, NYU Langone Medical Center, NY, USA.
authors.forename: Vanja
authors.initials: V
authors.lastname: Sisirak
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Bardel
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Boschetti
authors.affiliation: Cancerology Research Center of Lyon, Inserm U1052 - CNRS UMR5286, Lyon, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bendriss-Vermare
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Defrance
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.|Cancerology Research Center of Lyon, Inserm U1052 - CNRS UMR5286, Lyon, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Dubois
authors.affiliation: International Center for Infectiology Research (CIRI), Lyon, France.|Inserm U1111, Lyon, France.|CNRS UMR5308, Lyon, France.|Ecole Normale Superieure, Lyon, France.|Universite Claude Bernard Lyon 1, Lyon, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Kaiserlian
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D006706:Homeostasis; D006801:Humans; D007108:Immune Tolerance; D007114:Immunization; D007070:Immunoglobulin A / Q000378:metabolism*; D017578:Immunoglobulin Class Switching; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010950:Plasma Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D000073940:Transcription Factor 4
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D007070:Immunoglobulin A; C090743:Tcf4 protein, mouse; D000073940:Transcription Factor 4
keywords: IgA; Intestinal homeostasis; Oral immunization; Plasma cells; Plasmacytoid DCs