title: Mechanisms of fate decision and lineage commitment during haematopoiesis.
abstract: Blood stem cells need to both perpetuate themselves (self-renew) and differentiate into all mature blood cells to maintain blood formation throughout life. However, it is unclear how the underlying gene regulatory network maintains this population of self-renewing and differentiating stem cells and how it accommodates the transition from a stem cell to a mature blood cell. Our current knowledge of transcriptomes of various blood cell types has mainly been advanced by population-level analysis. However, a population of seemingly homogenous blood cells may include many distinct cell types with substantially different transcriptomes and abilities to make diverse fate decisions. Therefore, understanding the cell-intrinsic differences between individual cells is necessary for a deeper understanding of the molecular basis of their behaviour. Here we review recent single-cell studies in the haematopoietic system and their contribution to our understanding of the mechanisms governing cell fate choices and lineage commitment.
authors.affiliation: Department of Haematology, University of Cambridge, Cambridge, UK.|Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.|Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK.
authors.forename: Ana
authors.initials: A
authors.lastname: Cvejic
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D004843:Epistasis, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D059010:Single-Cell Analysis / Q000379:methods