title: Rapid acquisition of high-affinity DNA aptamer motifs recognizing microbial cell surfaces using polymer-enhanced capillary transient isotachophoresis.
abstract: We present a polymer-enhanced capillary transient isotachophoresis (PectI) selection methodology for acquisition of high-affinity (kinetically inert) DNA aptamers capable of recognizing distinct microbial cell surfaces, which requires only a single electrophoretic separation between particles (free cells and cells bound with aptamers) and molecules (unbound or dissociated DNA) in free solution.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan. shingo@mail.saitama-u.ac.jp.
authors.forename: Shingo
authors.initials: S
authors.lastname: Saito
authors.forename: Kazuki
authors.initials: K
authors.lastname: Hirose
authors.forename: Maho
authors.initials: M
authors.lastname: Tsuchida
authors.forename: Koji
authors.initials: K
authors.lastname: Wakui
authors.forename: Keitaro
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Masami
authors.initials: M
authors.lastname: Shibukawa
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry*; D001483:Base Sequence; D001681:Biological Assay; D057625:Isotachophoresis; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs; D013997:Time Factors
chemical_list: D052157:Aptamers, Nucleotide