title: Structural peculiarity and antithrombin binding region profile of mucosal bovine and porcine heparins.
abstract: The major compositional differences between bovine mucosal heparin (BMH) and the currently employed porcine mucosal heparin (PMH) have been reported to essentially consist of reduced 6-O-sulfation of the glucosamine residues in BMH and somewhat lower 2-O-sulfation of the iduronate residues in PMH. The present work is based on direct comparison of several BMH and PMH commercial preparations. A combined study by 2D (heteronuclear single quantum coherence, HSQC) NMR and ion-pair reversed-phase high performance liquid chromatography (IPRP-HPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) on the heparins, extended to the analysis of their heparinases digests and fractions separated by affinity chromatography on antithrombin (AT), confirmed the previously reported lower degree of 6-O-sulfation and showed lower 3-O-sulfated glucosamine content in BMH. More detailed studies allowed the identification of structural variants of AT-binding region (ATBR) structural variants, showing higher content of the N-sulfated components in BMH than in PMH.
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy. Electronic address: naggi@ronzoni.it.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Naggi
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gardini
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Pedrinola
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: Mauri
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Urso
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Alekseeva
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Benito
authors.initials: B
authors.lastname: Casu
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cassinelli
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Guerrini
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-990, Curitiba, PR, Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.affiliation: Kin Master Produtos Químicos Ltda. Passo Fundo, RS, Brazil.
authors.forename: Valentina
authors.initials: V
authors.lastname: Baigorria
authors.affiliation: Istituto di Ricerche Chimiche e Biochimiche G. Ronzoni, Via Giuseppe Colombo 81, 20133 Milano, Italy.
authors.forename: Giangiacomo
authors.initials: G
authors.lastname: Torri
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites / Q000502:physiology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013552:Swine
chemical_list: D000991:Antithrombins; D006493:Heparin
keywords: ATIII affinity chromatography; Bovine heparins; Liquid chromatography; Mass spectrometry; Nuclear magnetic resonance