title: Cytotoxic Clerodane Diterpenoids from the Leaves of Casearia grewiifolia.
abstract: Two new clerodane diterpenoids (1 and 2) and the known compound caseanigrescen D (3) were isolated from the leaves of Casearia grewiifolia by bioassay-guided fractionation. Their structures were determined by analyses of MS and 2D NMR data. The absolute configurations of 1 and 3 were established by analysis of X-ray diffraction data. Compounds 1-3 were evaluated for their cytotoxicity against four cancer cell lines, KB (mouth epidermal carcinoma cells), HepG-2 (human liver hepatocellular carcinoma cells), LU-1 (human lung adenocarcinoma cells), and MCF-7 (human breast cancer cells). Caseagrewifolin B (2) had inhibitory activity against KB and HepG-2 cell lines with IC50 values of 6.2 to 7.0 μM, respectively. When tested against the normal cells (NIH/3T3), caseagrewifolin B (2) exhibited a significant selective inhibition against cancer cells in comparison with the normal cells. Caseanigrescen D (3) was cytotoxic against four cancer cell lines; however it had no selective inhibition compared with normal cells.
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Ha T T
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Ngan B
authors.initials: NB
authors.lastname: Truong
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Huong T M
authors.initials: HT
authors.lastname: Doan
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR 2301, Université Paris-Sud , 91198, Gif-sur-Yvette, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Litaudon
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR 2301, Université Paris-Sud , 91198, Gif-sur-Yvette, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Retailleau
authors.affiliation: Institute of Biotechnology of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Thao T
authors.initials: TT
authors.lastname: Do
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Hung V
authors.initials: HV
authors.lastname: Nguyen
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Minh V
authors.initials: MV
authors.lastname: Chau
authors.affiliation: Institute of Marine Biochemistry of the Vietnam Academy of Science and Technology (VAST) , 18 Hoang Quoc Viet Road, Cau Giay, Hanoi, Vietnam.
authors.forename: Cuong V
authors.initials: CV
authors.lastname: Pham
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D037821:Casearia / Q000737:chemistry*; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007624:KB Cells; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D014744:Vietnam
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane; C000605751:caseagrewifolin B; C000605752:caseagrewiifolin A