title: Multilocus sequence analysis for Mycoplasma synoviae molecular genotyping.
abstract: The aim of this study was to identify molecular techniques which enable clear discrimination between Mycoplasma synoviae isolates for improved epidemiology. Multilocus sequence analysis (MLSA) of 6 M. synoviae loci was conducted for genotyping of isolates with previously determined 5'-vlhA sequences. Sequencing of three polymorphic genes (5'-vlhA, cysP and nanH) enables good discrimination between isolates with different genotypes. Such a genotyping scheme revealed 10 distinct genotypes, which were confirmed by sequencing of an additional three loci of the M. synoviae genome. Epidemiologically linked strains formed clusters with the same genotypes which clearly differed between clusters. MLSA used in this study is a promising tool for epidemiology of M. synoviae isolates, but it should be evaluated by further investigations using a much higher number of M. synoviae strains.
authors.affiliation: a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
authors.forename: I
authors.initials: I
authors.lastname: Cizelj
authors.affiliation: a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
authors.forename: R L
authors.initials: RL
authors.lastname: Berčič
authors.affiliation: b Veterinary Faculty , University of Ljubljana , Ljubljana , Slovenia.
authors.forename: B
authors.initials: B
authors.lastname: Slavec
authors.affiliation: a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
authors.forename: M
authors.initials: M
authors.lastname: Narat
authors.affiliation: a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
authors.forename: P
authors.initials: P
authors.lastname: Dovč
authors.affiliation: a Department of Animal Science, Biotechnical Faculty , University of Ljubljana , Domžale , Slovenia.
authors.forename: D
authors.initials: D
authors.lastname: Benčina
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002645:Chickens; D005838:Genotype; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000662:veterinary*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D001426:Bacterial Proteins