title: 1,25(OH)2D3 attenuates TGF-β1/β2-induced increased migration and invasion via inhibiting epithelial-mesenchymal transition in colon cancer cells.
abstract: 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has been reported to inhibit proliferation and migration of multiple types of cancer cells. However, the mechanism underlying its anti-metastasis effect is not fully illustrated. In this study, the effect of 1,25(OH)2D3 on TGF-β1/β2-induced epithelial-mesenchymal transition (EMT) is tested in colon cancer cells. The results suggest that 1,25(OH)2D3 inhibited TGF-β1/β2-induced increased invasion and migration of in SW-480 and HT-29 cells. 1,25(OH)2D3 also inhibited the cadherin switch in SW-480 and HT-29 cells. TGF-β1/β2-induced increased expression of EMT-related transcription factors was also inhibited by 1,25(OH)2D3. 1,25(OH)2D3 also inhibited the secretion of MMP-2 and MMP-9 and increased expression of F-actin induced by TGF-β1/β2 in SW-480 cells. Taken together, this study suggests that the suppression of EMT might be one of the mechanisms underlying the anti-metastasis effect of 1,25(OH)2D3 in colon cancer cells.
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Shanwen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Zuo
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Ju
authors.initials: J
authors.lastname: Ma
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Junling
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Chen
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Yisheng
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China.
authors.forename: Yucun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Division of General Surgery, Peking University First Hospital, Peking University, 8 Xi ShiKu Street, Beijing 100034, PR China. Electronic address: wangpengyuan2014@126.com.
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Wang
mesh_terms: D000199:Actins / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000517:prevention & control*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000199:Actins; D015820:Cadherins; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D014815:Vitamins; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D002117:Calcitriol
keywords: 1,25(OH)2D3; Colon cancer; EMT; HT-29; SW-480; VDR