title: Plant-microbe rhizosphere interactions mediated by Rehmannia glutinosa root exudates under consecutive monoculture.
abstract: Under consecutive monoculture, the biomass and quality of Rehmannia glutinosa declines significantly. Consecutive monoculture of R. glutinosa in a four-year field trial led to significant growth inhibition. Most phenolic acids in root exudates had cumulative effects over time under sterile conditions, but these effects were not observed in the rhizosphere under monoculture conditions. It suggested soil microbes might be involved in the degradation and conversion of phenolic acids from the monocultured plants. T-RFLP and qPCR analysis demonstrated differences in both soil bacterial and fungal communities during monoculture. Prolonged monoculture significantly increased levels of Fusarium oxysporum, but decreased levels of Pseudomonas spp. Abundance of beneficial Pseudomonas spp. with antagonistic activity against F. oxysporum was lower in extended monoculture soils. Phenolic acid mixture at a ratio similar to that found in the rhizosphere could promote mycelial growth, sporulation, and toxin (3-Acetyldeoxynivalenol, 15-O-Acetyl-4-deoxynivalenol) production of pathogenic F. oxysporum while inhibiting growth of the beneficial Pseudomonas sp. W12. This study demonstrates that extended monoculture can alter the microbial community of the rhizosphere, leading to relatively fewer beneficial microorganisms and relatively more pathogenic and toxin-producing microorganisms, which is mediated by the root exudates.
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Linkun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Juanying
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Huang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Hongmiao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.|Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, P. R. China.
authors.forename: Wenxiong
authors.initials: W
authors.lastname: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D005670:Fusarium / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000032:analysis / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D032267:Rehmannia / Q000166:cytology / Q000382:microbiology*; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D062385:Hydroxybenzoates; D012336:RNA, Ribosomal, 16S; C017616:phenolic acid