title: Methyl 9-Oxo-(10E,12E)-octadecadienoate Isolated from Fomes fomentarius Attenuates Lipopolysaccharide-Induced Inflammatory Response by Blocking Phosphorylation of STAT3 in Murine Macrophages.
abstract: Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin E2 through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-α and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor κB, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Choe
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Young-Joo
authors.initials: YJ
authors.lastname: Yi
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Myeong-Seok
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Bong-Sik
authors.initials: BS
authors.lastname: Yun
authors.affiliation: Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental & Bioresources, Chonbuk National University, Iksan 54596, Korea.
authors.forename: Sang-Myeong
authors.initials: SM
authors.lastname: Lee
keywords: Anti-inflammatory effect; Fomes fomentarius; Macrophages; STAT3