title: Up-regulation of CYLD enhances Listeria monocytogenes induced apoptosis in THP-1 cells.
abstract: Listeria monocytogenes (Lm), a facultative anaerobic gram-positive bacterium, causes listeriosis. Immune cell apoptosis is considered to be one pathogenic factor for listeriosis. As a deubiquitinase, CYLD is an important regulator both in innate immune response and apoptosis by negatively modulating NF-κB pathway. However the role of CYLD in Lm induced apoptosis remains unclear. Here we found that CYLD is significantly up-regulated in macrophages upon its infection. There is a moderate decrease in Lm proliferation and apoptotic cells in siRNA-induced CYLD knockdown THP-1 cells. Thereby CYLD may be involved in cell apoptosis mediated by Lm infection and its proliferation.
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China.
authors.forename: Changzhi
authors.initials: C
authors.lastname: Xu
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.affiliation: The Central Laboratory of Binhu Hospital, The Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: The Clinical Laboratory of Binhu Hospital, The Third Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Du
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China.
authors.forename: Xiangfang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China. Electronic address: zhbc@ahu.edu.cn.
authors.forename: Buchang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei, Anhui 230601, PR China. Electronic address: david.weaver.t@gmail.com.
authors.forename: David
authors.initials: D
authors.lastname: Weaver
mesh_terms: D017209:Apoptosis / Q000276:immunology; D002460:Cell Line; D000074749:Deubiquitinating Enzyme CYLD; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D006801:Humans; D007113:Immunity, Innate; D008089:Listeria monocytogenes / Q000276:immunology* / Q000378:metabolism* / Q000472:pathogenicity; D008088:Listeriosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D016328:NF-kappa B / Q000276:immunology / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D015533:Transcriptional Activation; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D016328:NF-kappa B; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; C409984:CYLD protein, human; D000074749:Deubiquitinating Enzyme CYLD
keywords: Apoptosis; CYLD; Listeria monocytogenes