title: Improvement of Sporobolomyces ruberrimus carotenoids production by the use of raw glycerol.
abstract: The red yeast Sporobolomyces ruberrimus H110 was able to use glycerol as a carbon source. The highest concentration (0.51gL(-1)) and productivity (0.0064gL(-1)h(-1)) of carotenoids were achieved when raw glycerol from biodiesel production, containing around 1gL(-1) of fatty acids, was used as the carbon source, which represented increases of 27% and 1.5×, respectively, in relation to pure glycerol. Mass spectrometry analysis led to the identification of four carotenoids in the fermented samples, torularhodin, torulene, β-carotene and γ-carotene. The use of raw glycerol also enhanced the proportion of torularhodin (69% against 59% in pure glycerol). The addition of individual fatty acids (palmitic, stearic, oleic and linoleic acids) to pure glycerol resulted in increases between 15% and 25% in maximum concentration and between 1.6× and 2.0× in productivity of carotenoids. The presence of palmitic and oleic acids increased the torularhodin proportion to 66%.
authors.affiliation: Industrial Biotechnology Program, Universidade Positivo, Prof. Pedro Viriato Parigot de Souza Street, 5300, Campo Comprido, Curitiba, Paraná, Brazil. Electronic address: ligiacardoso@up.edu.br.
authors.forename: L A C
authors.initials: LA
authors.lastname: Cardoso
authors.affiliation: LRGP - BioProMo Plateforme Technologique Sciences du Vivant et Santé - 13, Bois de la Champelle Street, 54500 Vandoeuvre-les-Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Jäckel
authors.affiliation: Industrial Biotechnology Program, Universidade Positivo, Prof. Pedro Viriato Parigot de Souza Street, 5300, Campo Comprido, Curitiba, Paraná, Brazil.
authors.forename: S G
authors.initials: SG
authors.lastname: Karp
authors.affiliation: LRGP - BioProMo Plateforme Technologique Sciences du Vivant et Santé - 13, Bois de la Champelle Street, 54500 Vandoeuvre-les-Nancy, France.
authors.forename: X
authors.initials: X
authors.lastname: Framboisier
authors.affiliation: LRGP - BioProMo Plateforme Technologique Sciences du Vivant et Santé - 13, Bois de la Champelle Street, 54500 Vandoeuvre-les-Nancy, France.
authors.forename: I
authors.initials: I
authors.lastname: Chevalot
authors.affiliation: LRGP - BioProMo Plateforme Technologique Sciences du Vivant et Santé - 13, Bois de la Champelle Street, 54500 Vandoeuvre-les-Nancy, France.
authors.forename: I
authors.initials: I
authors.lastname: Marc
mesh_terms: D001487:Basidiomycota / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors; D002338:Carotenoids / Q000096:biosynthesis*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D005285:Fermentation; D005990:Glycerol / Q000737:chemistry*; D008041:Linoleic Acids / Q000737:chemistry; D013058:Mass Spectrometry; D009829:Oleic Acids / Q000737:chemistry; D010169:Palmitic Acids / Q000737:chemistry; D013229:Stearic Acids / Q000737:chemistry; D019207:beta Carotene / Q000096:biosynthesis*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D019207:beta Carotene; D002338:Carotenoids; C078922:torulene; C078923:torularhodin; D005990:Glycerol
keywords: Carotenoids; Fatty acids; Raw glycerol; Sporobolomyces ruberrimus; Torularhodin