title: Production of Structured Triacylglycerols Containing Palmitic Acids at sn-2 Position and Docosahexaenoic Acids at sn-1, 3 Positions.
abstract: Docosahexaenoic acid supplementation has been shown well-established health benefits that justify their use as functional ingredients in healthy foods and nutraceutical products. Structured triacylglycerols rich in 1,3-docosahexenoyl-2-palmitoyl-sn-glycerol were produced from algal oil (Schizochytrium sp) which was prepared by a two-step process. Novozym 435 lipase was used to produce tripalmitin. Tripalmitin was then used to produce the final structured triacylglycerol (STAG) through interesterification reactions using Lipozyme RM IM. The optimum conditions for the enzymatic reaction were a mole ratio of tripalmitin/fatty acid ethyl esters 1:9, 60°C, 10% enzyme load (wt % of substrates), 10 h; the enzymatic product contained 51.6% palmitic acid (PA), 30.13% docosahexaenoic acid (DHA, C22:6 n-3) and 5.33% docosapentanoic acid (DPA, C22:5 n-3), 12.15% oleic acid (OLA). This STAG can be used as a functional ingredient in dietary supplementation to provide the benefits of DHA.
authors.affiliation: College of Food Science and Engineering, Ocean University of China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Liu
authors.forename: Yongli
authors.initials: Y
authors.lastname: Guo
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Sun
authors.forename: Xu
authors.initials: X
authors.lastname: Jie
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Li
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000032:analysis*; D004800:Enzymes, Immobilized; D004951:Esterification; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry; D010169:Palmitic Acids / Q000032:analysis*; D014280:Triglycerides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D010169:Palmitic Acids; D014280:Triglycerides; D004281:Docosahexaenoic Acids; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase