title: Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.
abstract: Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India.
authors.forename: Mythili
authors.initials: M
authors.lastname: Tummalapalli
authors.affiliation: Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, UMR 5305 CNRS-UCBL, Lyon Cedex 07, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Berthet
authors.affiliation: Laboratory of Tissue Biology and Therapeutic Engineering, IBCP, UMR 5305 CNRS-UCBL, Lyon Cedex 07, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Verrier
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India.
authors.forename: B L
authors.initials: BL
authors.lastname: Deopura
authors.affiliation: Department of Chemistry, Jamia Hamdard, New Delhi 62, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Alam
authors.affiliation: Bioengineering Lab, Department of Textile Technology, Indian Institute of Technology, New Delhi 16, India. Electronic address: bgupta@textile.iitd.ernet.in.
authors.forename: Bhuvanesh
authors.initials: B
authors.lastname: Gupta
mesh_terms: D000504:Aloe / Q000737:chemistry*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival; D003474:Curcumin / Q000737:chemistry* / Q000494:pharmacology*; D065546:Drug Liberation; D005780:Gelatin / Q000737:chemistry*; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D010368:Pectins / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001672:Biocompatible Materials; D010368:Pectins; D005780:Gelatin; D003474:Curcumin
keywords: Biocomposite; Oxidized pectin; Wound healing