title: MiR-122 partly mediates the ochratoxin A-induced GC-2 cell apoptosis.
abstract: Ochratoxin A (OTA) is a mycotoxin which has been shown to be nephrotoxic, hepatotoxic, and immunotoxic to animals, and mainly exists in the mildew grain. MicroRNAs (miRNAs) regulate a wide variety of cellular processes. However, the toxic effects of OTA on the germ cell and whether miRNAs mediate the effects of OTA-induced GC-2 cell apoptosis are still not clear. In the present study, OTA treatment resulted in a dose-dependent increase apoptosis in GC-2 cells. MiR-122 was increased in the OTA-treated GC-2 cells. It showed that Bcl-w was down-regulated after OTA treatment, and caspase-3 was obviously activated. Cyclin G1 (CCNG1) was significantly decreased, and inversely the expression of p53 was increased. Inhibition of miR-122 partly relieved the OTA-induced GC-2 cell apoptosis. These results indicate that OTA induces GC-2 cell apoptosis by causing the increase of caspase-3 activity and that miR-122 partly mediates the OTA-induced cell apoptosis.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Animal Genetics and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory of Food Safety and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, PR China.
authors.forename: Liye
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Laboratory of Food Safety and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Yiheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Laboratory of Food Safety and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
authors.affiliation: Medical Center of Reproductive and Genetics, Peking University First Hospital, Beijing, PR China. Electronic address: bdyychenliang@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China. Electronic address: luohaoshu@cau.edu.cn.
authors.forename: Haoshu
authors.initials: H
authors.lastname: Luo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D053148:Caspase 3 / Q000378:metabolism; D002478:Cells, Cultured; D056766:Cyclin G1 / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008969:Molecular Sequence Data; D009793:Ochratoxins / Q000633:toxicity*; D013090:Spermatocytes / Q000187:drug effects* / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: C534702:CCNG1 protein, human; D056766:Cyclin G1; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D009793:Ochratoxins; D016159:Tumor Suppressor Protein p53; C025589:ochratoxin A; D053148:Caspase 3
keywords: Cell apoptosis; GC-2 cell; Ochratoxin A; miR-122