title: Toxicological analysis and anti-inflammatory effects of essential oil from Piper vicosanum leaves.
abstract: This study assessed the anti-inflammatory effects of the essential oil from Piper vicosanum leaves (OPV) and evaluated the toxicological potential of this oil through acute toxicity, genotoxicity and mutagenicity tests. The acute toxicity of OPV was evaluated following oral administration to female rats at a single dose of 2 g/kg b.w. To evaluate the genotoxic and mutagenic potential, male mice were divided into five groups: I: negative control; II: positive control; III: 500 mg/kg of OPV; IV: 1000 mg/kg of OPV; V: 2000 mg/kg of OPV. The anti-inflammatory activity of OPV was evaluated in carrageenan-induced pleurisy and paw edema models in rats. No signs of acute toxicity were observed, indicating that the LD50 of this oil is greater than 2000 mg/kg. In the comet assay, OPV did not increase the frequency or rate of DNA damage in groups treated with any of the doses assessed compared to that in the negative control group. In the micronucleus test, the animals treated did not exhibit any cytotoxic or genotoxic changes in peripheral blood erythrocytes. OPV (100 and 300 mg/kg) significantly reduced edema formation and inhibited leukocyte migration analyzed in the carrageenan-induced edema and pleurisy models. These results show that OPV has anti-inflammatory potential without causing acute toxicity or genotoxicity.
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Débora Regina
authors.initials: DR
authors.lastname: Hoff Brait
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Márcia Soares
authors.initials: MS
authors.lastname: Mattos Vaz
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Jucicléia
authors.initials: J
authors.lastname: da Silva Arrigo
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Luciana Noia
authors.initials: LN
authors.lastname: Borges de Carvalho
authors.affiliation: Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Souza de Araújo
authors.affiliation: Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Juliana Miron
authors.initials: JM
authors.lastname: Vani
authors.affiliation: Mato Grosso do Sul State University (UEMS), Dourados, MS, Brazil.
authors.forename: Jonas
authors.initials: J
authors.lastname: da Silva Mota
authors.affiliation: Mato Grosso do Sul State University (UEMS), Dourados, MS, Brazil.
authors.forename: Claudia Andrea Lima
authors.initials: CA
authors.lastname: Cardoso
authors.affiliation: Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
authors.forename: Rodrigo Juliano
authors.initials: RJ
authors.lastname: Oliveira
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Fábio Juliano
authors.initials: FJ
authors.lastname: Negrão
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil.
authors.forename: Cândida Aparecida Leite
authors.initials: CA
authors.lastname: Kassuya
authors.affiliation: School of Health Sciences, Federal University of Grande Dourados, Dourados, MS, Brazil; Department of Morphology, Institute of Biosciences of Botucatu, UNESP - Univ. Estadual Paulista, Botucatu, SP, Brazil. Electronic address: ariellearena@ibb.unesp.br.
authors.forename: Arielle Cristina
authors.initials: AC
authors.lastname: Arena
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002351:Carrageenan; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009822:Oils, Volatile / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D008517:Phytotherapy; D031701:Piper / Q000737:chemistry / Q000633:toxicity; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018515:Plant Leaves; D010938:Plant Oils / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal; D010998:Pleurisy / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D018570:Risk Assessment; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D002351:Carrageenan
keywords: Acute toxicity; Essential oil; Genotoxicity; Inflammation; Piper vicosanum; Piperaceae