title: Population distribution and genetic relatedness in Indian Fusarium udum isolates based on ribosomal internal transcribe spacer and elongation factor.
abstract: With the objective to study the geographical distribution pattern and pathotype classification, isolates from 12 major pigeonpea growing states of India were examined at morphological and molecular levels. Two DNA based internal transcribe spacer (ITS) region derived primers FDP 3 (ITS1/ITS2), FDP 25 (mRNA, LOC100383610) and two elongation factors FDP 4 (F98-BKR5) and FDP 29 (M9968PY) were employed to genetically differentiate the isolates. As a result, each marker system gave an average of 3 alleles/marker. The higher efficiency of ITS over EF-1α marker was revealed using detailed comparative analysis that included various parameters like gene diversity index, effective marker ratio, and marker index. Neighbour Joining tree analysis grouped the isolates into three major clusters and showed narrow existence of genetic divergence. Combination of genotyping data with pathological measurements indicates dominance of variant 1 in the Central zone, South zone and North East Plain Zone, while North East Plain Zone and North West Plain Zone were largely dominated by variants 2 and 1, with strong possibility of evolving other variants. The present study would help in identifying specific isolate and patterns of its distribution in various pigeonpea growing regions thereby enhancing the scope for precise resistance breeding for crop improvement.
authors.forename: Khela R
authors.initials: KR
authors.lastname: Soren
authors.forename: Priyanka
authors.initials: P
authors.lastname: Gangwar
authors.forename: R G
authors.initials: RG
authors.lastname: Chaudhary
authors.forename: Subhojit
authors.initials: S
authors.lastname: Datta
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005670:Fusarium / Q000235:genetics*; D007194:India; D010802:Phylogeny
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer