title: Right Time, Right Place: Probing the Functions of Organelle Positioning.
abstract: The proper spatial arrangement of organelles underlies many cellular processes including signaling, polarization, and growth. Despite the importance of local positioning, the precise connection between subcellular localization and organelle function is often not fully understood. To address this, recent studies have developed and employed different strategies to directly manipulate organelle distributions, such as the use of (light-sensitive) heterodimerization to control the interaction between selected organelles and specific motor proteins, adaptor molecules, or anchoring factors. We review here the importance of subcellular localization as well as tools to control local organelle positioning. Because these approaches allow spatiotemporal control of organelle distribution, they will be invaluable tools to unravel local functioning and the mechanisms that control positioning.
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands.
authors.forename: Petra
authors.initials: P
authors.lastname: van Bergeijk
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands.
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
authors.affiliation: Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands. Electronic address: l.kapitein@uu.nl.
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Kapitein
mesh_terms: D048708:Cell Growth Processes / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D002477:Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D015388:Organelles / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction; D013347:Subcellular Fractions; D013997:Time Factors
keywords: intracellular transport; motor proteins; optogenetics; organelle positioning; protein heterodimerization