title: Beneficial effect of D-allose for isolated islet culture prior to islet transplantation.
abstract: BACKGROUND
  Pretransplant restoration of islets damaged during isolation remains to be solved. In this study, we examined the effect of D-allose on islets isolated from rat pancreata prior to islet transplantation.
  
  
  METHODS
  Rat islets isolated from fresh pancreata were cultured overnight in Roswell Park Memorial Institute 1640 solution in the absence (group 1) or presence (group 2) of D-allose. Then we assessed stimulation index of insulin, and cure rate after islet transplantation to diabetic nude mice. We also measured malondialdehyde level and caspase 3 activity of islets after the overnight culture for assessment of the oxidative stress and the apoptosis.
  
  
  RESULTS
  D-allose significantly improved insulin secretion of islets. The stimulation index in group 2 was significantly higher than in group 1. Cure rate after transplantation in group 2 was higher than in group 1 especially in the first week. The malondialdehyde level in group 2 was significantly lower than in group 1. But the caspase 3 activities in both groups did not differ.
  
  
  CONCLUSIONS
  D-allose treatment of isolated islet culture prior to transplantation restored islet function and increased successful transplant rate. The results of this study suggested that D-allose improved function of damaged islets through its anti-oxidative activity.
authors.affiliation: Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan. kashiwa@med.kagawa-u.ac.jp.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kashiwagi
authors.affiliation: Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Asano
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Noguchi
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Sui
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Akram
authors.initials: A
authors.lastname: Hossain
authors.affiliation: Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Akamoto
authors.affiliation: Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Okano
authors.affiliation: Department of Cell Physiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Tokuda
authors.affiliation: Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan.
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D005947:Glucose / Q000627:therapeutic use*; D007515:Islets of Langerhans / Q000187:drug effects; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013237:Stereoisomerism
chemical_list: D008315:Malondialdehyde; C002055:allose; D053148:Caspase 3; D005947:Glucose
keywords: D-allose; Islet transplantation; Rare sugar