title: Overexpression of the long non-coding RNA PVT1 is correlated with leukemic cell proliferation in acute promyelocytic leukemia.
abstract: BACKGROUND
  Acute promyelocytic leukemia (APL) is associated with chromosomal translocation t(15;17), which results in the proliferation of morphologically abnormal promyelocytes. Gain of supernumerary copies of the 8q24 chromosomal region, which harbors MYC and PVT1, has been shown to be the most common secondary alteration in human APL. Increased MYC can accelerate the development of myeloid leukemia in APL. However, the role that the expression of the long non-coding RNA (lncRNA) PVT1 plays in the pathogenesis of APL remains largely unknown.
  
  
  RESULTS
  In this study, we first analyzed the lncRNA PVT1 expression level in peripheral blood cells from 28 patients with de novo APL, and significantly upregulated PVT1 was found in APL patients compared with healthy donors. We then observed significantly lower MYC and PVT1 expression during all-trans retinoic acid (ATRA)-induced differentiation and cell cycle arrest in the APL cell line. MYC knockdown in NB4 cells led to PVT1 downregulation. Moreover, PVT1 knockdown by RNA interference led to suppression of the MYC protein level, and cell proliferation was inhibited.
  
  
  CONCLUSIONS
  Our findings reveal that the lncRNA PVT1 may play an important role in the proliferation of APL cells and may be useful for future therapeutic management.
authors.affiliation: First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.|Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China.
authors.forename: Chengwu
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China.
authors.forename: Xibao
authors.initials: X
authors.lastname: Yu
authors.affiliation: First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.|Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lai
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Institute of Hematology, Medical College, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.|Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, China. yangqiuli@hotmail.com.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000970:Antineoplastic Agents / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; C489427:MYC protein, human; C521123:PVT1 long-non-coding RNA, human; D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; D014212:Tretinoin