title: Dual Action of miR-125b As a Tumor Suppressor and OncomiR-22 Promotes Prostate Cancer Tumorigenesis.
abstract: MicroRNAs (miRs) are a novel class of small RNA molecules, the dysregulation of which can contribute to cancer. A combinatorial approach was used to identify miRs that promote prostate cancer progression in a unique set of prostate cancer cell lines, which originate from the parental p69 cell line and extend to a highly tumorigenic/metastatic M12 subline. Together, these cell lines are thought to mimic prostate cancer progression in vivo. Previous network analysis and miR arrays suggested that the loss of hsa-miR-125b together with the overexpression of hsa-miR-22 could contribute to prostate tumorigenesis. The dysregulation of these two miRs was confirmed in human prostate tumor samples as compared to adjacent benign glandular epithelium collected through laser capture microdissection from radical prostatectomies. In fact, alterations in hsa-miR-125b expression appeared to be an early event in tumorigenesis. Reverse phase microarray proteomic analysis revealed ErbB2/3 and downstream members of the PI3K/AKT and MAPK/ERK pathways as well as PTEN to be protein targets differentially expressed in the M12 tumor cell compared to its parental p69 cell. Relevant luciferase+3'-UTR expression studies confirmed a direct interaction between hsa-miR-125b and ErbB2 and between hsa-miR-22 and PTEN. Restoration of hsa-miR-125b or inhibition of hsa-miR-22 expression via an antagomiR resulted in an alteration of M12 tumor cell behavior in vitro. Thus, the dual action of hsa-miR-125b as a tumor suppressor and hsa-miR-22 as an oncomiR contributed to prostate tumorigenesis by modulations in PI3K/AKT and MAPK/ERK signaling pathways, key pathways known to influence prostate cancer progression.
authors.affiliation: Department of Biochemistry and Molecular Biology, Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: William T
authors.initials: WT
authors.lastname: Budd
authors.affiliation: Department of Biochemistry and Molecular Biology, Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia, United States of America.|Department of Forensic Science, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Seashols-Williams
authors.affiliation: Department of Biochemistry and Molecular Biology, Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: Gene C
authors.initials: GC
authors.lastname: Clark
authors.affiliation: Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: Danielle
authors.initials: D
authors.lastname: Weaver
authors.affiliation: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, United States of America.
authors.forename: Valerie
authors.initials: V
authors.lastname: Calvert
authors.affiliation: Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, Virginia, United States of America.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Petricoin
authors.affiliation: Department of Pathology, VCU Medical Center, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: Ema A
authors.initials: EA
authors.lastname: Dragoescu
authors.affiliation: American International Biotechnology, Richmond, Virginia, United States of America.
authors.forename: Katherine
authors.initials: K
authors.lastname: O'Hanlon
authors.affiliation: Department of Biochemistry and Molecular Biology, Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia, United States of America.
authors.forename: Zendra E
authors.initials: ZE
authors.lastname: Zehner
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D063646:Carcinogenesis / Q000378:metabolism*; D045744:Cell Line, Tumor; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D016147:Genes, Tumor Suppressor; D006801:Humans; D008297:Male; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D051058:Oncogene Protein v-akt / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011467:Prostate / Q000166:cytology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; C531888:MIRN125 microRNA, human; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases