title: Contribution of Avian Salmonella enterica Isolates to Human Salmonellosis Cases in Constantine (Algeria).
abstract: An epidemiological investigation was carried out on one hundred Salmonella isolates from broiler farms, slaughterhouses, and human patients in the Constantine region of Algeria, in order to explore the contribution of avian strains to human salmonellosis cases in this region over the same period of time. The isolates were characterized by phenotypic as well as genotypic methods. A large variety of antimicrobial resistance profiles was found among human isolates, while only seven profiles were found among avian isolates. Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR), Insertion Sequence 200-PCR (IS200-PCR), and Pulsed Field Gel Electrophoresis (PFGE) resulted in the allocation of the isolates to 16, 20, and 34 different profiles, respectively. The 3 genotyping methods led to complementary results by underlining the clonality of some serovars with the diffusion and persistence of a single clone in the Constantine area as well as stressing the polymorphism present in isolates belonging to other serovars, indicating the diversity of potential reservoirs of nontyphoidal Salmonella. Altogether, our results seem to indicate that nontyphoidal avian Salmonella may play an important role in human salmonellosis in the Constantine region.
authors.affiliation: Laboratory of Health Management and Animal Production, Veterinary Sciences Department, Constantine Mentouri University, Constantine, Algeria ; Alfort National Veterinary School, Paris-Est University, 94704 Maisons-Alfort Cedex, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Elgroud
authors.affiliation: Antimicrobial Resistance Unit, Maisons-Alfort Laboratory for Food Safety, ANSES, Paris-Est University, 94701 Maisons-Alfort Cedex, France.
authors.forename: Sophie A
authors.initials: SA
authors.lastname: Granier
authors.affiliation: Antimicrobial Resistance Unit, Maisons-Alfort Laboratory for Food Safety, ANSES, Paris-Est University, 94701 Maisons-Alfort Cedex, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Marault
authors.affiliation: Hygiene and Quality of Poultry and Pig Products Unit, Anses Ploufragan Laboratory, European University of Brittany, 22440 Ploufragan, France.
authors.forename: Annaëlle
authors.initials: A
authors.lastname: Kerouanton
authors.affiliation: Service de Microbiologie-Bactériologie, C.H.U. Ibn Badis, Constantine, Algeria.
authors.forename: Abdesslem
authors.initials: A
authors.lastname: Lezzar
authors.affiliation: Service de Microbiologie-Bactériologie, C.H.U. Ibn Badis, Constantine, Algeria.
authors.forename: Chafia
authors.initials: C
authors.lastname: Bouzitouna-Bentchouala
authors.affiliation: Antimicrobial Resistance Unit, Maisons-Alfort Laboratory for Food Safety, ANSES, Paris-Est University, 94701 Maisons-Alfort Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Brisabois
authors.affiliation: Alfort National Veterinary School, Paris-Est University, 94704 Maisons-Alfort Cedex, France ; Antimicrobial Resistance Unit, Maisons-Alfort Laboratory for Food Safety, ANSES, Paris-Est University, 94701 Maisons-Alfort Cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Millemann
mesh_terms: D000003:Abattoirs; D000462:Algeria; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology; D004058:Diffusion; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology; D012480:Salmonella Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D019779:Salmonella enterica / Q000235:genetics*; D012703:Serotyping
chemical_list: D000900:Anti-Bacterial Agents