title: Insights on profiling of phorbol, deoxyphorbol, ingenol and jatrophane diterpene esters by high performance liquid chromatography coupled to multiple stage mass spectrometry.
abstract: This paper reports our effort to develop a comprehensive HPLC-MS(n)-based dereplication strategy for phorbol ester (PE), deoxyphorbol ester (dPE) and ingenol ester (IE) profiling in plant extracts. This strategy is composed of two sequential analysis exploiting specific hybrid triple quadrupole/linear ion trap instrument modes. A first run was performed using a multiple reaction monitoring (MRM) mode targeting fragmentation of PE and dPE/IE coupled with the acquisition of MS(2) spectrum for the ions at m/z 311 and m/z 313, respectively. A second run was then completed based on precursor ion scan mode (PIS) and automatic MS(2) acquisition for each quasimolecular ion. The developed approach was used to investigate ten Euphorbia extracts showing bioactivity against chikungunya virus replication. Experiments allowed partial annotation of three dPE/IE but no PE was detected. Results suggested that other types of diterpene esters displayed PE- and dPE/IE-like fragmentations. The study of jatrophane ester (JE) standards by CID fragmentation using low and high resolution mass spectrometry confirmed this hypothesis, highlighting challenges and difficulties of diterpene esters profiling within plant extracts. Nonetheless, the present LC-MS(n) method can be easily adapted to profile other types of diterpene esters.
authors.affiliation: Laboratoire de Chimie de Produits Naturels, UMR CNRS SPE 6134, University of Corsica, Corte 20250, France; Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, LabEx CEBA, University Paris-Saclay, 1 avenue de la terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: Louis-Félix
authors.initials: LF
authors.lastname: Nothias-Scaglia
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, LabEx CEBA, University Paris-Saclay, 1 avenue de la terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schmitz-Afonso
authors.affiliation: Laboratoire de Chimie de Produits Naturels, UMR CNRS SPE 6134, University of Corsica, Corte 20250, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Renucci
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, LabEx CEBA, University Paris-Saclay, 1 avenue de la terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Roussi
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, LabEx CEBA, University Paris-Saclay, 1 avenue de la terrasse, 91198 Gif-sur-Yvette, France.
authors.forename: David
authors.initials: D
authors.lastname: Touboul
authors.affiliation: Laboratoire de Chimie de Produits Naturels, UMR CNRS SPE 6134, University of Corsica, Corte 20250, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Costa
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS, UPR 2301, LabEx CEBA, University Paris-Saclay, 1 avenue de la terrasse, 91198 Gif-sur-Yvette, France. Electronic address: marc.litaudon@cnrs.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Litaudon
authors.affiliation: Laboratoire de Chimie de Produits Naturels, UMR CNRS SPE 6134, University of Corsica, Corte 20250, France. Electronic address: paolini@univ-corse.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Paolini
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis / Q000737:chemistry; D004952:Esters / Q000032:analysis*; D028481:Euphorbia / Q000737:chemistry*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D004224:Diterpenes; D004952:Esters; D010936:Plant Extracts; C530345:jatrophane; C008125:ingenol
keywords: Deoxyphorbol; Diterpene esters; HPLC-MS(n); Ingenol; Jatrophane; Phorbol