title: Analysis of sterigmatocystin in cereals, animal feed, seeds, beer and cheese by immunoaffinity column clean-up and HPLC and LC-MS/MS quantification.
abstract: A method is reported for the analysis of sterigmatocystin in various food and feed matrices using a commercial sterigmatocystin immunoaffinity column (IAC) for sample clean-up prior to HPLC analysis by UV with mass spectrometric detection (LC-MS/MS). Cereals (wheat, oats, rye, maize and rice), sunflower seeds and animal feed were spiked with sterigmatocystin at levels from 0.75 to 50 µg kg(-1) to establish method performance. Using acetonitrile/water extraction followed by IAC clean-up, and analysis by HPLC with detection at 325 nm, recoveries ranged from 68% to 106%, with repeatability from 4.2% to 17.5%. The limit of quantification with UV detection in these matrices was 1.5 µg kg(-1). For the analysis of beer and cheese the sample preparation prior to IAC clean-up was changed to accommodate the different properties of the matrix, prior to analysis by LC-MS/MS. For beer and cheese spiked at 5.0 µg kg(-1) the recoveries were 94% and 104%, and precision (RSDs) were 1.9% and 2.9% respectively. The limits of quantification by LC-MS/MS in beer and cheese were 0.02 and 0.6 µg kg(-1) respectively. The sterigmatocystin IAC was demonstrated to provide an efficient clean-up of various matrices to enable this mycotoxin to be determined by either HPLC with UV detection or LC-MS/MS.
authors.affiliation: a Research & Development, Sales & Marketing and Technical Services Departments, R-Biopharm Rhone Ltd , Glasgow G20 OXA , UK.
authors.forename: Elaine
authors.initials: E
authors.lastname: Marley
authors.affiliation: a Research & Development, Sales & Marketing and Technical Services Departments, R-Biopharm Rhone Ltd , Glasgow G20 OXA , UK.
authors.forename: Phyllis
authors.initials: P
authors.lastname: Brown
authors.affiliation: a Research & Development, Sales & Marketing and Technical Services Departments, R-Biopharm Rhone Ltd , Glasgow G20 OXA , UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mackie
authors.affiliation: a Research & Development, Sales & Marketing and Technical Services Departments, R-Biopharm Rhone Ltd , Glasgow G20 OXA , UK.
authors.forename: Carol
authors.initials: C
authors.lastname: Donnelly
authors.affiliation: a Research & Development, Sales & Marketing and Technical Services Departments, R-Biopharm Rhone Ltd , Glasgow G20 OXA , UK.
authors.forename: Joyce
authors.initials: J
authors.lastname: Wilcox
authors.affiliation: b Food Science and Nutrition Institute, Faculty of Agricultural, Food and Environmental Science, Universita Cattolica del Sacro Cuore , Via Emilia Parmense, 84 - 29122 , Piacenza , Italy.
authors.forename: Amedeo
authors.initials: A
authors.lastname: Pietri
authors.affiliation: c Food Chemistry, Fera Science Ltd. , Sand Hutton , York YO41 1LZ , UK.
authors.forename: Susan
authors.initials: S
authors.lastname: Macdonald
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001515:Beer / Q000032:analysis*; D002611:Cheese / Q000032:analysis*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry* / Q000187:drug effects; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D057230:Limit of Detection; D009183:Mycotoxins / Q000302:isolation & purification* / Q000494:pharmacology; D015203:Reproducibility of Results; D013241:Sterigmatocystin / Q000302:isolation & purification* / Q000494:pharmacology; D053719:Tandem Mass Spectrometry
chemical_list: D009183:Mycotoxins; D013241:Sterigmatocystin
keywords: LC-MS/MS analysis; beer; cereals; cheese; feed; immunoaffinity column; sterigmatocystin