title: An Association between Single Nucleotide Polymorphisms of Lys751Gln ERCC2 Gene and Ovarian Cancer in Polish Women.
abstract: Aim. The aim of this study was to evaluate the role of the Lys751Gln (rs13181) ERCC2 gene polymorphism in clinical parameters and the risk for development of ovarian cancer. Material and Methods. The study consisted of 430 patients with ovarian cancer (mean age: 53.2 ± 10.11) and 430 healthy subjects (mean age: 50.31 ± 18.21). Analysis of the gene polymorphisms was performed using the PCR-based restriction fragment length polymorphism (PCR-RFLP). The odds ratios (ORs) and 95% confidence intervals (CIs) for each genotype and allele were calculated. Results. The results obtained indicate that the genotype Gln/Gln is associated with an increased risk of ovarian cancer (OR 5.01; 95% CI 3.37-7.43; p < 0.0001). Association of Lys751Gln polymorphism with histological grading showed increased ERCC2 Gln/Gln (OR = 6.96; 95% CI 3.41-14.21; p < 0.0001) genotype in grading 1 as well as Gln allele overrepresentation (OR = 4.98; 95% CI 3.37-7.40; p < 0.0001) in G1 ovarian patients. Finally, with clinical FIGO staging under evaluation, an increase in ERCC2 Gln/Gln homozygote frequencies in staging I and Gln allele frequencies in SI were observed. Conclusion. On the basis of these results, we conclude that ERCC2 gene polymorphism Lys751Gln may be associated with an increased risk of ovarian carcinoma.
authors.affiliation: Department of Obstetrics and Gynaecology, Regional Hospital in Kalisz, Poznańska 79, 62-800 Kalisz, Poland.
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Michalska
authors.affiliation: Department of Obstetrics and Gynaecology, Regional Hospital in Kalisz, Poznańska 79, 62-800 Kalisz, Poland ; Cathedral of Mother's and Child's Health, Poznan University of Medical Sciences, Polna 33, 60-535 Poznań, Poland.
authors.forename: Dariusz
authors.initials: D
authors.lastname: Samulak
authors.affiliation: Laboratory of Cancer Genetics, Department of Pathology, Institute of Polish Mother's Memorial Hospital, Rzgowska 281/289, 93-338 Łódź, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Romanowicz
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital, Polna 33, 60-535 Poznań, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Sobkowski
authors.affiliation: Laboratory of Cancer Genetics, Department of Pathology, Institute of Polish Mother's Memorial Hospital, Rzgowska 281/289, 93-338 Łódź, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Smolarz