title: Ameliorative effect of quercetin against arsenic-induced sperm DNA damage and daily sperm production in adult male rats.
abstract: In this study, the protective effect of quercetin was evaluated against arsenic induced reproductive ailments in male rats. For this purpose, male rats (n = 5/group) weighing 180-250 g were used. First group served as control, second group received arsenic (50 ppm) in drinking water. Third group was treated with quercetin (50 mg/kg) alone, while fourth group received arsenic + quercetin. All treatments were carried out for 49 days. After treatment, animals were killed by decapitation; testis and epididymis were dissected out. Right epididymis was minced immediately for comet assay, while left epididymis was processed for histology. Similarly, right testis was homogenized for estimation of daily sperm production (DSP) and detection of metal concentration. The results of our research revealed that arsenic treatment did not cause any significant change in body weight and testicular volume. Quercetin treatment significantly prevented tissue deposition of arsenic within the testis. Arsenic treatment caused a significant reduction in DSP, however, in the arsenic + quercetin-treated group and quercetin alone-treated group, DSP was significantly high as compared to the arsenic-treated group. Histological study of epididymis showed empty lumen in arsenic-treated group while in arsenic + quercetin-treated group and quercetin alone-treated group, lumen were filled with sperm and were comparable to control. Sperm DNA damage, induced by arsenic, was significantly reversed toward control levels by supplementation of quercetin. These results suggest that quercetin not only prevents deposition of arsenic in tissues, but can also protect the sperm DNA damage.
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Jahan
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Saima
authors.initials: S
authors.lastname: Rehman
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Hizb
authors.initials: H
authors.lastname: Ullah
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Asma
authors.initials: A
authors.lastname: Munawar
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Qurat Ul
authors.initials: QU
authors.lastname: Ain
authors.affiliation: a Reproductive Physiology Lab, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan.
authors.forename: Tariq
authors.initials: T
authors.lastname: Iqbal
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D018053:Arsenites / Q000493:pharmacokinetics / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D011794:Quercetin / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D017670:Sodium Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D013054:Spectrophotometry, Atomic; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D018053:Arsenites; D004785:Environmental Pollutants; D017670:Sodium Compounds; C017947:sodium arsenite; D011794:Quercetin
keywords: Arsenic; DNA damage; epididymis; quercetin; sperm production