title: Cathelicidin modulates the function of monocytes/macrophages via the P2X7 receptor in a teleost, Plecoglossus altivelis.
abstract: Cathelicidins (CATHs) are a family of endogenous antimicrobial peptides that are capable of both direct bacteria-killing and immunomodulatory effects. P2X7 receptor (P2X7R) is a mediator of CATH in mammalian immune cells. Here, we studied the function and regulation of CATH in head kidney-derived monocytes/macrophages (MO/MФ) from ayu, Plecoglossus altivelis. We investigated the chemotaxis of MO/MФ in response to ayu CATH (PaCATH), and found that PaCATH had a dose-dependent effect on MO/MФ chemotaxis with the optimal concentration of 10.0 μg/ml. The qPCR and Western blot analysis revealed that PaCATH inhibited the expression of ayu P2X7R (PaP2X7R) at both mRNA and protein levels. Knockdown of the PaP2X7R expression in ayu MO/MФ by RNA interference not only significantly inhibited the chemotactic effect of PaCATH on MO/MФ, but also obviously reduced the effect of PaCATH on the phagocytosis, bacteria-killing, respiratory burst, and cytokine expression of ayu MO/MФ. Our study revealed that the immunomodulatory effect of fish CATH is mediated by P2X7R.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Li
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Xin-Jiang
authors.initials: XJ
authors.lastname: Lu
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Ming-Yun
authors.initials: MY
authors.lastname: Li
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China. Electronic address: jchen1975@163.com.
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D036441:Osmeriformes / Q000235:genetics / Q000276:immunology*; D058486:Receptors, Purinergic P2X7 / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D029941:Fish Proteins; D058486:Receptors, Purinergic P2X7; D054804:Cathelicidins
keywords: Ayu; Cathelicidin; Chemotaxis; Monocytes/macrophages function; P2X7 receptor