title: A new type of biocompatible fluorescent probe AFN for fixed and live cell imaging of intracellular lipid droplets.
abstract: We discovered a new class of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts. The salient features of the probe are its visible excitation maximum, aqueous compatibility, selectivity and remarkable stability (for more than a week) in live cells, even better than commercially available Nile red.
authors.affiliation: Fluorescent Lab, Medicinal and Process Chemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, India. atul_goel@cdri.res.in.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Sharma
authors.forename: Shahida
authors.initials: S
authors.lastname: Umar
authors.forename: Parmita
authors.initials: P
authors.lastname: Kar
authors.forename: Kavita
authors.initials: K
authors.lastname: Singh
authors.forename: Monika
authors.initials: M
authors.lastname: Sachdev
authors.forename: Atul
authors.initials: A
authors.lastname: Goel
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D005449:Fluorenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D066292:Lipid Droplets / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D059629:Signal-To-Noise Ratio
chemical_list: D001672:Biocompatible Materials; D005449:Fluorenes; D005456:Fluorescent Dyes; C000613212:azafluorenone