title: The complete mitochondrial genome of the king horseshoe bat (Rhinolophus rex) using next-generation sequencing and Sanger sequencing.
abstract: The king horseshoe bat (Rhinolophus rex) is endemic to China. Here, we characterized the complete mitochondrial genome of R. rex using next-generation sequencing and Sanger sequencing. The mitogenome is 16 845 bp in length and contains 22 tRNAs, two rRNAs, 13 protein-coding genes, and a non-coding control region. Our traditional Sanger sequencing verified that mtDNA sequences generated from next-generation sequencing are reliable. The mitogenome of R. rex will be useful for the conservation genetics of this endangered species.
authors.affiliation: a Institute of Molecular Ecology and Evolution, SKLEC, East China Normal University, Shanghai , China.
authors.forename: Huizhen
authors.initials: H
authors.lastname: Shi
authors.affiliation: a Institute of Molecular Ecology and Evolution, SKLEC, East China Normal University, Shanghai , China.
authors.forename: Shuyi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: a Institute of Molecular Ecology and Evolution, SKLEC, East China Normal University, Shanghai , China.
authors.forename: Xiuguang
authors.initials: X
authors.lastname: Mao
mesh_terms: D000818:Animals; D001482:Base Composition; D002685:Chiroptera / Q000145:classification / Q000235:genetics*; D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018387:Codon, Initiator; D018388:Codon, Terminator; D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Bat; Rhinolophus rex; de novo assembly; mitogenome