title: [Detection of Molecular Cytogenetic Aberrations by Fluorescence in Situ Hybridization in Different Bone Marrow Samples of Multiple Myeloma].
abstract: OBJECTIVE
  To detect the molecular cytogenetic abnormalities in different bone marrow samples of multiple myeloma by using fluorescence in situ hybridization (FISH) technology. The bone marrow cells from 48 cases of MM were taken for sorting the plasma cells using CD138 magnetic beads, and the biopsy tissue from 10 cases of MM was taken and embedded in parafin, then the 2 kinds of samples were detected by using FISH. D13s319/RB1, 1q21/P53, IgH, IgH/FGFR3, IgH/MAF probes were detected in 58 patients with new diagnosed multiple myeloma (MM) by FISH technology.
  
  
  RESULTS
  Fluorescent signals were both seen in 2 different types of bone marrow samples and cytogenetic aberrations were detected in 30/58 (51.7%) patients, 29.3% (17 out of 58) cases had both D13S319 and RB1 deletion. The positive rates of P53 deletion, 1q21 amplification and IgH rearrangement were 12%, 27.6% and 20.7%, respectively. Only 7 cases (23.3%) had one cytogenetic abnormality, other 23 (76.7%) cases all had 2 to 5 kinds of different abnormalities.
  
  
  CONCLUSIONS
  More than half of MM patients have cytogenetic change, and most of them are complex chromosomal abnormality. The different kinds of samples can expand the useful extension of FISH technology and acquire more cytogenetic information for clinician.
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Lian-Yong
authors.initials: LY
authors.lastname: Xi
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Ji-Jun
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Hematology, Peking University Third Hospital, Beijing 100191, China. E-mail: xiaoyank@yahoo.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Ke
mesh_terms: D001854:Bone Marrow Cells; D002465:Cell Movement; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D006801:Humans; D018189:Immunomagnetic Separation; D017404:In Situ Hybridization, Fluorescence; D009101:Multiple Myeloma / Q000235:genetics*; D010950:Plasma Cells