title: Microsatellite markers for the Jaera albifrons species complex (marine isopods).
abstract: BACKGROUND
  The Jaera albifrons complex contains five species of marine isopods (J. albifrons, J. praehirsuta, J. ischiosetosa, J. forsmani, and J. posthirsuta). These species, occurring on the shores of the North-Atlantic Ocean, are partially reproductively isolated by barriers due to sexual isolation (mate choice), genetic incompatibilities, and ecological specialization. Microsatellite loci would be useful for parentage-based analyses of sexual selection and studies of genetic structure in the context of speciation.
  
  
  RESULTS
  Twenty-four microsatellite markers were developed for J. albifrons using pyrosequencing of enriched libraries. Patterns of polymorphisms were analyzed in 49 J. albifrons adult males sampled in two populations from Brittany (Western France). The average number of alleles per locus was 4.73 ± 2.45 and the average gene diversity was 0.55 ± 0.23. Most markers also successfully amplified in the three sibling species J. praehirsuta, J. ischiosetosa, and J. forsmani.
  
  
  CONCLUSIONS
  These polymorphic and cross-amplifiable markers will be useful for population genetics and parentage studies in the J albifrons complex.
authors.affiliation: CNRS, Station Biologique de Roscoff, Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Team Diversity and Connectivity of Coastal Marine Landscapes, 29680, Roscoff, France. ambre.ribardiere@sb-roscoff.fr.
authors.forename: Ambre
authors.initials: A
authors.lastname: Ribardière
authors.affiliation: CNRS, Station Biologique de Roscoff, Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Team Diversity and Connectivity of Coastal Marine Landscapes, 29680, Roscoff, France. thomas.broquet@sb-roscoff.fr.
authors.forename: Thomas
authors.initials: T
authors.lastname: Broquet
authors.affiliation: CNRS, Station Biologique de Roscoff, Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Team Diversity and Connectivity of Coastal Marine Landscapes, 29680, Roscoff, France. claire.daguin@sb-roscoff.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Daguin-Thiébaut
mesh_terms: D000818:Animals; D001267:Atlantic Ocean; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005602:France; D005787:Gene Frequency; D005838:Genotype; D033321:Isopoda / Q000145:classification / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004247:DNA