title: Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates.
abstract: In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.
authors.affiliation: Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland. peter.owens@nuigalway.ie.
authors.forename: Peter
authors.initials: P
authors.lastname: Owens
authors.affiliation: School of Physics, National University Ireland, University Road, Galway, Ireland. N.Phillipson2@nuigalway.ie.
authors.forename: Nigel
authors.initials: N
authors.lastname: Phillipson
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, Singapore. Jayakumar_Perumal@sbic.a-star.edu.sg.
authors.forename: Jayakumar
authors.initials: J
authors.lastname: Perumal
authors.affiliation: School of Physics, National University Ireland, University Road, Galway, Ireland. gerard.oconnor@nuigalway.ie.
authors.forename: Gerard M
authors.initials: GM
authors.lastname: O'Connor
authors.affiliation: Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, Singapore. Malini_Olivo@sbic.a-star.edu.sg.
authors.forename: Malini
authors.initials: M
authors.lastname: Olivo
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D066246:ErbB Receptors / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015122:Mercaptopurine / Q000737:chemistry; D008853:Microscopy / Q000379:methods; D049329:Nanostructures / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D000814:Aniline Compounds; D055910:Antibodies, Immobilized; D015415:Biomarkers; D013438:Sulfhydryl Compounds; D016159:Tumor Suppressor Protein p53; D012834:Silver; C064316:4-aminothiophenol; D015122:Mercaptopurine; D066246:ErbB Receptors
keywords: Raman spectroscopy; biomarker; protein sensing; surface-enhanced Raman scattering