title: Integrative taxonomy helps to reveal the mask of the genus Gynandropaa (Amphibia: Anura: Dicroglossidae).
abstract: Species of the genus Gynandropaa within the family Dicroglossidae are typical spiny frogs whose taxonomic status has long been in doubt. We used integrative methods, involving morphological and molecular analyses, to elucidate the phylogenetic relationships, and to determine identities and the geographic distribution of each valid species. We obtained DNA sequence data of 5 species of Gynandropaa (complete sequences of the mitochondrial NADH dehydrogenase subunit 2 [ND2] gene, and 890 bp of 12S rRNA and 16S rRNA partial sequences) from 37 localities (including the topotypes of 5 described species) and constructed Bayesian and maximum-likelihood trees to examine the patterns of phylogeography. A total of 28 morphological variables were taken on 624 specimens. Three clades with clear geographic patterns were recognized: clade C (from south-western Sichuan Province and central Yunnan Province), clade E (western Guizhou Province and eastern to central Yunnan Province) and clade W (western to southern Yunnan Province). Integrating morphological characteristics and distribution information, the clades W, E and C represent Gynandropaa yunnanensis, G. phrynoides and G. sichuanensis, respectively. We draw the following conclusions: (i) the taxon G. phrynoides, formerly evaluated as a junior synonym of G. yunnanensis, is revalidated herein at the rank of species; (ii) G. liui is a junior synonym of G. sichuanensis; and (iii) G. yunnanensis is a valid species while G. bourreti is probably a subspecies of G. yunnanensis, with the distribution range from Vietnam to southern Yunnan Province. This study clears up the taxonomic status of Gynandropaa and provides important information for understanding the evolution and conservation of these spiny frogs.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.|College of Life Science, Sichuan University, Chengdu, China.|University of Chinese Academy of Sciences, Beijing, China.|Key Laboratory of Southwest China Wildlife Resources Conservation, China West Normal University, Ministry of Education, Nanchong, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Hu
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Life Science, Sichuan University, Chengdu, China.
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Song
authors.affiliation: Key Laboratory of Southwest China Wildlife Resources Conservation, China West Normal University, Ministry of Education, Nanchong, China.
authors.forename: Caiquan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001001:Anura / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D002681:China; D058974:Phylogeography; D012335:RNA, Ribosomal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C075526:RNA, ribosomal, 12S
keywords: amphibians; mitochondrial DNA; morphological traits; spiny frogs; systematics