title: PTTG regulates the metabolic switch of ovarian cancer cells via the c-myc pathway.
abstract: Human pituitary tumor-transforming gene (PTTG) is a proto-oncogene involved in the development, invasion, and metastasis of many types of cancer, including ovarian cancer. However, little is known about the role of PTTG in the metabolic shift of ovarian cancer cells. In our study, we show that PTTG expression was positively correlated with the differentiation degree of ovarian cancer tissue. In addition, PTTG suppression by specific shRNA could inhibit the proliferation of ovarian cancer cells A2780 and SKOV-3. Furthermore, aerobic glycolysis was suppressed and oxidative phosphorylation was increased in ovarian cancer cells after PTTG suppression. We further found that the expression of c-myc and several crucial enzymes involved in aerobic glycolysis (e.g., PKM2, LDHA, and glucose transporter 1 (GLUT-1)) were downregulated by PTTG knockwown. Overexpression of c-myc could prevent the metabolic shift induced by PTTG knockwown. Together, our findings suggest that the oncogene PTTG promotes the progression of ovarian cancer cells, and its loss resists tumor development, in part, by regulating cellular metabolic reprogramming that supports cell growth and proliferation via c-myc pathway.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.|Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Wanxing
authors.initials: W
authors.lastname: Duan
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Xuqi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qian
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Guangren Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
authors.forename: Qiaoshan
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Department of Neurosurgery, Baylor Scott & White Health, Temple, Texas, 76502, USA.
authors.forename: Erxi
authors.initials: E
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000235:genetics; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064249:Securin / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*
chemical_list: C000715470:MAS1 protein, human; C489427:MYC protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D017382:Reactive Oxygen Species; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human
keywords: aerobic glycolysis; human pituitary tumor-transforming gene (PTTG); metabolic switch; ovarian cancer; oxidative phosphorylation