title: The biological effects of vanadyl curcumin and vanadyl diacetylcurcumin complexes: the effect on structure, function and oxidative stability of the peroxidase enzyme, antibacterial activity and cytotoxic effect.
abstract: Curcumin has multiple pharmacological effects, but it has poor stability. Complexation of curcumin with metals improves its stability. Here, the effects of vanadyl curcumin and vanadyl diacetylcurcumin on the function and structure of horseradish peroxidase enzyme were evaluated by spectroscopic techniques. Cytotoxic effect of the complexes was also assessed on MCF-7 breast cancer, bladder and LNCaP prostate carcinoma cell line. The results showed that the complexes improve catalytic activity of HRP, and also increase its tolerance against the oxidative condition. The result also indicated that the affinity of HRP for hydrogen peroxide substrate decreases, while the affinity increases for phenol substrate. Circular dichroism and fluorescence spectroscopies showed that compactness of the enzyme structure around the catalytic heme group and the distance between the heme group and tryptophan residue decreases after the binding. The antibacterial and cytotoxic results indicated that the complexes have anticancer potential, but they have no considerable antibacterial activity.
authors.affiliation: a Department of Biological Sciences , Institute for Advanced Studies in Basic Sciences (IASBS) , Zanjan , Iran .
authors.forename: Akram
authors.initials: A
authors.lastname: Hamidi
authors.affiliation: a Department of Biological Sciences , Institute for Advanced Studies in Basic Sciences (IASBS) , Zanjan , Iran .
authors.forename: Leila
authors.initials: L
authors.lastname: Hassani
authors.affiliation: b Department of Chemistry , Institute for Advanced Studies in Basic Sciences (IASBS) , Zanjan , Iran , and.
authors.forename: Fakhrossadat
authors.initials: F
authors.lastname: Mohammadi
authors.affiliation: a Department of Biological Sciences , Institute for Advanced Studies in Basic Sciences (IASBS) , Zanjan , Iran .
authors.forename: Parisa
authors.initials: P
authors.lastname: Jahangoshayi
authors.affiliation: c Chemistry Department, Faculty of Sciences, Persian Gulf University , Bushehr , Iran.
authors.forename: Khosro
authors.initials: K
authors.lastname: Mohammadi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D003474:Curcumin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D004795:Enzyme Stability; D006801:Humans; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017968:Vanadium Compounds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D017968:Vanadium Compounds; D010544:Peroxidases; D003474:Curcumin
keywords: Cytotoxicity; horseradish peroxidase; oxidative stability; vanadyl curcumin; vanadyl diacetylcurcumin