title: Biodegradation of di-n-Butyl Phthalate by Achromobacter sp. Isolated from Rural Domestic Wastewater.
abstract: A bacterial strain W-1, isolated from rural domestic wastewater, can utilize the environmental hormone di-n-butyl phthalate (DBP) as the sole carbon and energy source. The isolated bacterium species was confirmed to belong to the genus Achromobacter based on its 16S rRNA gene sequence. The results of substrate utilization tests showed that the strain W-1 could utilize other common phthalates and phenol. High-performance liquid chromatography analysis revealed that the optimal conditions for DBP degradation were pH 7.0, 35 °C, and an agitation rate of 175 rpm. Under these conditions, 500 mg/L of DBP was completely degraded within 30 h. The effects of heavy metals (50 mg/L Cu(2+) and 500 mg/L Pb(2+)) and surfactants (100 mg/L SDS and 500 mg/L Tween 20) on DBP degradation were investigated. The results demonstrated that Cu(2+) and SDS severely inhibited DBP degradation and Pb(2+) weakly inhibited DBP degradation, while Tween 20 greatly enhanced DBP degradation. Furthermore, phthalate degradation genes were found to be located on a plasmid present in Achromobacter sp. W-1.
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. dcjin@rcees.ac.cn.
authors.forename: Decai
authors.initials: D
authors.lastname: Jin
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. kongxiaozaikeda@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Kong
authors.affiliation: Environmental Protection Bureau of Shijiazhuang City, Shijiazhuang 050021, China. lyj8026@163.com.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. zhbai@rcees.ac.cn.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. gqzhuang@rcees.ac.cn.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. xlzhuang@rcees.ac.cn.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Environmental Biotechnology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. yedeng@rcees.ac.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Deng
mesh_terms: D042402:Achromobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D062065:Wastewater / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D003993:Dibutyl Phthalate; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Achromobacter; biodegradation; di-n-butyl phthalate; environmental hormone; heavy metals; surfactants