title: Effect of overexpression of hypoxia-inducible factor-1α induced by hyperoxia in vivo in LNCaP tumors on tumor growth rate.
abstract: OBJECTIVE
  To study effect of overexpression of hypoxia-inducible factor-1α induced by hyperoxia in vivo in LNCaP tumors on tumor growth rate.
  
  
  METHODS
  The prostate cancer LNCaP cells were inoculated in the abdomen of mice. All the mice were randomly placed in the gas chamber with different oxygen content. The groups were divided as follows: twelve mice in hypoxia group, sixteen mice in normoxia group, ten mice in hyperoxia group. After 28 d of treatment, the mice were weighed, the blood samples were taken from the left ventricle, and the tumor was isolated and weighed. Tumor growth, angiogenesis and vascularization, HIF-1α expression and intracellular signal transduction molecules expression in each group of xenografts were detected and analyzed by using Western blotting and immunofluorescence and determination of hemoglobin.
  
  
  RESULTS
  Comparison of the growth of xenografts in each group showed that, the xenografts growth of hypoxia group was more quickly than that of normoxia group. The difference was statistically significant (P = 0.004). The difference in xenografts growth between hyperoxia group compared and normoxia group was not statistically significant (P > 0.05). The expressions of HIF-1α, VEGF and VEGF-R of xenografts in hyperoxia group were significantly higher than those of normoxia group (P < 0.05). The expression of HIF-1α of xenografts in hypoxia group and normoxia group were similar. The blood growth rate of xenografts in hypoxia group (170%) was significantly higher than that of normoxia group (40%) (P < 0.05). The expression of Nrf2 of xenografts in hyperoxia group was significantly higher than that of normoxia group (P < 0.05).
  
  
  CONCLUSIONS
  When hyperoxia induces the overexpression of HIF-1α in LNCaP tumor, it will not affect tumor growth. It provides a new ideas and theoretical basis for the clinical treatment of prostate cancer.
authors.affiliation: Department of Neurology, The 4th Hospital of Hebei Medical University, Hebei 050011, China.
authors.forename: Tian-Yan
authors.initials: TY
authors.lastname: Zhang
authors.affiliation: Department of Pain Medicine and Rehabilitation, The 4th Hospital of Hebei Medical University, Hebei 050011, China. Electronic address: yangjuanli656@163.com.
authors.forename: Juan-Li
authors.initials: JL
authors.lastname: Yang
authors.affiliation: Department of Traditional Chinese Medicine, The 4th Hospital of Hebei Medical University, Hebei 050011, China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Huo
keywords: HIF-1α; Hyperoxia; Hypoxia