title: Thermus parvatiensis RL(T) sp. nov., Isolated from a Hot Water Spring, Located Atop the Himalayan Ranges at Manikaran, India.
abstract: A Gram negative, yellow pigmented, rod shaped bacterium designated as RL(T) was isolated from a hot water spring (90-98 °C) located at Manikaran in Northern India. The isolate grows at 60-80 °C (optimum, 70 °C) and at pH 7.0-9.0 (optimum pH 7.2). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA-DNA relatedness together indicate that the new isolate represents a novel species of the genus Thermus with closest affinity to Thermus thermophilus HB8(T) (99.5 %) followed by Thermus arciformis (96.4 %). A comparative analysis of partial sequences of housekeeping genes (HKG) further revealed that strain RL(T) is a novel species belonging to the genus Thermus. The melting G+C content of strain RL(T) was calculated as 68.7 mol%. The DNA-DNA relatedness value of strain RL(T) with its nearest neighbours (>97 %) was found to be less than 70 % indicating that strain RL(T) represents a novel species of the genus Thermus. MK-8 was the predominant respiratory quinone. The presence of characteristic phospholipid and glycolipid further confirmed that strain RL(T) belongs to the genus Thermus. The predominant fatty acids of strain RL(T) were iso-C17:0 (23.67 %) and iso-C15:0 (24.50 %). The results obtained after DNA-DNA hybridization, biochemical and physiological tests clearly distinguished strain RL(T) from its closely related species. Thus, strain RL(T) represents a novel species of the genus Thermus for which the name Thermus parvatiensis is proposed (=DSM 21745(T)= MTCC 8932(T)).
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Vatsala
authors.initials: V
authors.lastname: Dwivedi
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Kirti
authors.initials: K
authors.lastname: Kumari
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Rekha
authors.initials: R
authors.lastname: Kumari
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Charu
authors.initials: C
authors.lastname: Tripathi
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Pushp
authors.initials: P
authors.lastname: Lata
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Neha
authors.initials: N
authors.lastname: Niharika
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Roshan
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Aeshna
authors.initials: A
authors.lastname: Nigam
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Garg
authors.affiliation: Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi, 110007 India.
authors.forename: Rup
authors.initials: R
authors.lastname: Lal
keywords: Hot spring; Manikaran; Thermophile; Thermus parvatiensis