title: Enzyme-linked immunosorbent assay for triclocarban in aquatic environments.
abstract: A sensitive, competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of triclocarban (TCC) in waters and sediments. Haptens were synthesized by derivatizing the paraposition of a phenyl moiety of TCC. The synthesized hapten was then coupled to bovine thyroglobulin to be used as an immunogen, based on which, a high affinity monoclonal antibody 4D5 was produced with the hybridoma technique. Under the optimized conditions, using the monoclonal antibody, excellent performances of the assay were obtained: satisfactory sensitivity (IC50 (50% inhibition concentration) value, 0.43 ng/mL; limit of detection, 0.05 ng/mL); good linear range (0.05-10 ng/mL); and satisfactory accuracy (recoveries 70.7-107% in waters; 74.8-98.3% in sediments). Furthermore, TCC was found with the concentration ranging from not detected to 422.12 ng/L in waters and from 6.68 ng/g to 78.67 ng/g in sediments in Yunliang River, Ancient Canal and Hongqiao Port in Zhenjiang City. In conclusion ELISA could be applied for monitoring TCC in aquatic environments.
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zeng
authors.affiliation: School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Affiliated Hospital of Hebei University of Engineering, No. 81 Congtai Road, Handan City, Hebei Province 056029, China E-mail: gzik2010@126.com.
authors.forename: Jianxia
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Affiliated Hospital of Hebei University of Engineering, No. 81 Congtai Road, Handan City, Hebei Province 056029, China E-mail: gzik2010@126.com.
authors.forename: Zikuan
authors.initials: Z
authors.lastname: Gai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002224:Carbanilides / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019015:Geologic Sediments / Q000032:analysis; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D045483:Rivers / Q000737:chemistry; D013954:Thyroglobulin / Q000737:chemistry / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D002224:Carbanilides; D006241:Haptens; D014874:Water Pollutants, Chemical; D013954:Thyroglobulin; C009540:triclocarban