title: Identification and Characterization of the Cyclin-Dependent Kinases Gene Family in Silkworm, Bombyx mori.
abstract: Cyclin-dependent protein kinases (CDKs) play key roles at different checkpoint regulations of the eukaryotic cell cycle. However, only few studies of lepidoptera CDK family proteins have been reported so far. In this study, we performed the cDNA sequencing of 10 members of the CDK family in Bombyx mori. Gene structure analysis suggested that CDK12 and CDC2L1 owned two and three isoforms, respectively. Phylogenetic analysis showed that CDK genes in different species were highly conserved, implying that they evolved independently even before the split between vertebrates and invertebrates. We found that the expression levels of BmCDKs in 13 tissues of fifth-instar day 3 larvae were different: CDK1, CDK7, and CDK9 had a high level of expression, whereas CDK4 was low-level expressed and was detected only in the testes and fat body cells. Similar expression profiles of BmCDKs during embryo development were obtained. Among the variants of CDK12, CDK12 transcript variant A had the highest expression, and the expression of CDC2L1 transcript variant A was the highest among the variants of CDC2L1. It was shown from the RNAi experiments that the silencing of CDK1, CDK10, CDK12, and CDC2L1 could influence the cells from G0/G1 to S phase transition.
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Yinü
authors.initials: Y
authors.lastname: Li
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Shi
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Xingjian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Huipeng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Biotechnology Research Institute , Chinese Academy of Agricultural Sciences, Beijing, China .
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000196:embryology / Q000235:genetics*; D018844:Cyclin-Dependent Kinases / Q000145:classification / Q000235:genetics*; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D059467:Transcriptome
chemical_list: D018844:Cyclin-Dependent Kinases