title: Plasma miR-1, miR-208, miR-499 as potential predictive biomarkers for acute myocardial infarction: An independent study of Han population.
abstract: BACKGROUND
  It has been demonstrated that circulating muscle-enriched miR-1 and the cardiac-specific miR-208 and miR-499 might leak out of the necrotic myocardium and release into the circulation during the early stages of acute myocardial infarction (AMI). This study aims to investigate potential predictive value of the three miRNAs in AMI.
  
  
  METHODS
  Plasma samples were taken from 70 AMI patients and 72 healthy controls. The expression levels of target miRNAs were measured by qRT-PCR. Student's t test and χ(2) test were applied to test the significance of the differences between AMI patients and controls. Additionally, a meta-analysis about the prediction accuracy of the three miRNAs in AMI was performed to summarize all the results from available studies.
  
  
  RESULTS
  The expression of plasma miR-1, miR-208 and miR-499 were all significantly elevated in AMI patients compared with controls. By receiver operating characteristic (ROC) curve analysis, plasma miR-1, miR-208 and miR-499 were demonstrated to be potential biomarkers for the prediction of AMI with AUC values of 0.81, 0.72, and 0.88, respectively. The meta-analysis consisted of nine studies for miR-1, eight studies for miR-208 and eight studies for miR-499. The summary estimates of miR-1, miR-208 and miR-499 were 0.73, 0.80 and 0.83 for sensitivity, 0.82, 0.95 and 0.90 for specificity, 0.84, 0.89 and 0.91 for AUC, respectively.
  
  
  CONCLUSIONS
  In summary, we confirmed the predictive values of plasma miR-1, miR-208 and miR-499 in AMI. In contrast to miR-1, the cardiac-specific miR-208 and miR-499 were supposed to be more reliable as biomarkers in AMI screening and prediction.
authors.affiliation: Department of Cardiology, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cardiac Surgery, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Zhanwei
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Neurosurgery, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Tianshu
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, The Second Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Cardiology, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China.
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory, Beijing Shijiantan Hospital, Capital Medical University, Beijing 100038, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Department of Cardiology, The First Clinical Hospital of Harbin Medical University, Harbin 150081, Heilongjiang Province, China. Electronic address: drtianye01@yeah.net.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Cardiac Surgery, The Fourth Clinical Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China. Electronic address: zhan_jinjin@163.com.
authors.forename: Baichun
authors.initials: B
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000368:Aged; D044466:Asian People; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002681:China; D005260:Female; D006801:Humans; D008297:Male; D015201:Meta-Analysis as Topic; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis*; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C544547:MIRN1 microRNA, human; C550906:MIRN208 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs
keywords: Acute myocardial infarction; Diagnosis; Plasma; miR-1; miR-208; miR-499; microRNA