title: Neurofibromin Regulates Seizure Attacks in the Rat Pilocarpine-Induced Model of Epilepsy.
abstract: Studies have shown that neurofibromin (NF1) restricts GABA release at inhibitory synapses and regulates dendritic spine formation, which may play an important role in temporal lobe epilepsy (TLE). NF1 expression was detected by double-label immunofluorescence, immunohistochemistry, and western blot analysis in the brains of pilocarpine-induced epilepsy model rats at 6 h, 24 h, 72 h, 7 days, 14 days, 30 days, and 60 days after kindling. NF1 was localized primarily in the nucleus and cytoplasm of neurons. NF1 protein levels significantly increased in the chronic phase (from 7 days until 60 days) in this epileptic rat model. After NF1 expression was knocked down by specific siRNA, the effects of kindling with pilocarpine were evaluated on the 7th day after kindling. The onset latencies of pilocarpine-induced seizures were elevated, and the seizure frequency and duration were reduced in these rats. Our study demonstrates that NF1 promoted seizure attacks in rats with pilocarpine-induced epilepsy.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Min
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Kunyi
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Neurology, Xinhua Hospital of Hubei Province, Hubei, 430000, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jiamei
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Long
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Youyi Road 1#, Yuzhong County, Chongqing, 400016, China. zkebin2008@aliyun.com.
authors.forename: Kebin
authors.initials: K
authors.lastname: Zeng
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D015536:Down-Regulation; D004827:Epilepsy / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism; D016086:Lentivirus / Q000378:metabolism; D008297:Male; D025542:Neurofibromin 1 / Q000378:metabolism*; D010862:Pilocarpine; D017207:Rats, Sprague-Dawley; D011995:Recombination, Genetic / Q000235:genetics; D012640:Seizures / Q000378:metabolism* / Q000473:pathology
chemical_list: D025542:Neurofibromin 1; D010862:Pilocarpine
keywords: Neurofibromin; RNA interference; Rat; Seizure; siRNA