title: Effects of zinc oxide nanoparticles on bioaccumulation and oxidative stress in different organs of tilapia (Oreochromis niloticus).
abstract: Nano-size zinc oxide particles (ZnO NPs) are used in diverse industrial and commercial fields. However, the information from existing studies is not sufficient in evaluating the potential toxic effects of ZnO NPs. In this study, tilapia fish (Oreochromis niloticus) were exposed to different concentrations of small and large ZnO NPs in vivo. Accumulation in various organs/tissues (liver, gill, intestine, kidney, brain and muscle) and possible oxidative stress mechanisms were investigated comparatively. Fish were exposed to 1 and 10mg/L concentrations of small (10-30 nm) and large (100 nm) ZnO NPs semi-statically for 14 days. Both small and large ZnO NPs accumulated substantially in the tissues. Accumulation for the small ZnO NPs was significantly higher compared to larger NPs under same exposure regimes. Significant fluctuations were observed in antioxidant defense system biomarkers, including Superoxide dismutase (SOD), Catalase (CAT) and Glutathione (GSH) levels depending on particle size, exposure time and concentration. Lipid peroxidation measured with TBARS levels were higher in groups exposed to the suspensions of small ZnO NPs than that of large ZnO NPs and controls. These results imply that colloidal suspensions of small ZnO NPs induce elevated oxidative stress and toxic effects on tilapia compared to the larger NPs.
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Marine Sciences and Technology, 17100 Çanakkale, Turkey. Electronic address: hasankaya@comu.edu.tr.
authors.forename: Hasan
authors.initials: H
authors.lastname: Kaya
authors.affiliation: Istanbul University, Fisheries Faculty, 34470 Istanbul, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Aydın
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Biology, 17100 Çanakkale, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Gürkan
authors.affiliation: Canakkale Onsekiz Mart University, Faculty of Marine Sciences and Technology, 17100 Çanakkale, Turkey.
authors.forename: Sevdan
authors.initials: S
authors.lastname: Yılmaz
authors.affiliation: Tunceli University, Engineering Faculty, Department of Bioengineering, 62000 Tunceli, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ates
authors.affiliation: Tunceli University, Engineering Faculty, Department of Environmental Engineering, 62000 Tunceli, Turkey.
authors.forename: Veysel
authors.initials: V
authors.lastname: Demir
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, MS 39217, USA.
authors.forename: Zikri
authors.initials: Z
authors.lastname: Arslan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000187:drug effects; D005880:Gills / Q000187:drug effects; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000633:toxicity*; D009132:Muscles / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D017210:Tilapia / Q000378:metabolism*; D015034:Zinc Oxide / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D015034:Zinc Oxide
keywords: Accumulation; Oxidative stress; Tilapia (Oreochromis niloticus); Toxicity; ZnO nanoparticles