title: Preparation of hemoglobin (Hb) imprinted polymer by Hb catalyzed eATRP and its application in biosensor.
abstract: Molecularly imprinted polymer (MIP) was prepared on the surface of Au electrode by electrochemically mediated atom transfer radical polymerization (eATRP) with hemoglobin (Hb) both as catalyst and template molecule. Firstly, the condition for eATRP such as the potential, time and Hb concentration were selected and determined to be -0.51 V, 120 min and 20mg/mL, respectively. Further, the electrode modified with MIP (MIP/Au) was carefully examined by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscope (SEM). Finally, the MIP/Au electrode was used as a biosensor and successfully applied for the determination of Hb by differential pulse voltammetry (DPV) measurement. The results of experiments showed that the proposed biosensor displayed a broader linear range and a lower detection limit for Hb determination when it was compared to those Hb sensors based on MIP. The linear range was from 1.0 × 10(-10) to 1.0 × 10(1)mg/L with a detection limit of 7.8 × 10(-11)mg/L (S/N=3.3). In a word, the work of this paper established a useful way for the preparation and application of biosensor based on protein imprinted polymers.
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China. Electronic address: yuesun@lnnu.edu.cn.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Du
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Lan
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Yuejian
authors.initials: Y
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Chunliang
authors.initials: C
authors.lastname: Feng
authors.affiliation: School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D003217:Conductometry / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006454:Hemoglobins; D011108:Polymers
keywords: Biosensor; Electrochemically mediated atom transfer radical polymerization; Hemoglobin; Molecularly imprinted polymer