title: [Effect of Emodin Combined with AZT on the Proliferation and the Expression of BCL-2, NF-κB, TGF-β in the Leukemia Stem Cells-KG-1a cells].
abstract: OBJECTIVE
  To investigate the effect of Emodin combined with 3'-azido-3'-deoxythymidine (AZT) on the proliferation and apoptosis of concentrated leukemia stem cells (CLSC)-human acute myeloid leukemia KG-la cells and expression of BCL-2, NF-κB and TGF-β.
  
  
  METHODS
  The tumor stem cell-like subpopulation in human leukemia cell line KG-1a was enriched with 5-fluorouracil (5-FU). The CD34⁺ CD38⁻ subpopulation in the KG-1a cells was detected with flow cytometry, the cell proliferation was detected by MTT method to study the of Emodin and AZT in the CLSC. The cell apoptosis was analyzed by flow cytometry. The expression of NF-κB, BCL-2 and TGF-β mRNA and proteins were measured with RT-PCR and Western blot respectively.
  
  
  RESULTS
  As compared with cells treated with mentioned above drugs alone, the inhibition of proliferation potential and apoptosis rate of cells in combination group markedly increase with time and concentration dependent member (P < 0.01), the expression of NF-κB, BCL-2 and TGF-β mRNA and proteins decreased.
  
  
  CONCLUSIONS
  Emodin combined AZT can synergistically inhibit the proliferation, induce cell apoptosis, and down regulate the expression of NF-κB, BCL-2 and TGF-β mRNA and proteins in the CLSC, the possible mechanism of synergistic effect may be associated with inhibiton of BCL-2 activation and down-regulation of the expression of NF-κB, and TGF-β.
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wang
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.|Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China.
authors.forename: Zi-Jian
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China. E-mail: xiyaming02@163.com.
authors.forename: Ya-Ming
authors.initials: YM
authors.lastname: Xi
authors.affiliation: Gansu College of Traditional Chinese Medicine, Lanzhou 730000, Gansu Province, China. E-mail: chen72123@163.com.
authors.forename: Che
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Jia
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Hematology and Department of Hematology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Liu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D004642:Emodin / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: C000595707:BCL2 protein, human; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D015215:Zidovudine; D004642:Emodin