title: Effects of high-fat feeding on ectopic fat storage and postprandial lipid metabolism in mouse offspring.
abstract: OBJECTIVE
  Parental high-fat feeding was proposed to negatively impact metabolic health in offspring. Here, the ectopic fat storage in heart and liver in offspring was investigated, and the effects on mitochondrial function, de novo lipogenesis, and postprandial lipid metabolism were explored in detail.
  
  
  METHODS
  Male and female mice received either a high-fat (HF) or standard chow (LF) diet during mating, gestation and lactation. All offspring animals received the HF diet.
  
  
  RESULTS
  Abdominal visceral adipose tissue tended to be higher in HF/HF mice. Cardiac lipid content was also higher in the HF/HF mice (LF/HF vs.
  1.03% ± 0.08% vs. 1.33% ± 0.07% of water signal, P = 0.01). In contrast, hepatic lipid content tended to be lower in HF/HF mice compared to LF/HF mice. A severely disturbed postprandial lipid clearance was revealed in HF/HF mice by the results from the triglyceride (TG) tolerance tests (LF/HF vs.
  6,753 ± 2,213 vs. 14,367 ± 1,978 mmol l(-1)  min(-1) , P = 0.01) and (13) C-fatty acid retention test (LF/HF vs.
  2.73% ± 0.85% vs. 0.89% ± 0.26% retention from bolus, P = 0.04), which may underlie the lower hepatic lipid content.
  
  
  CONCLUSIONS
  Here it is shown that HF diet negatively impacts postprandial TG clearance in offspring and results in an overall metabolic unfavorable phenotype and ectopic lipid deposition in the heart and in visceral storage sites.
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Petronella A
authors.initials: PA
authors.lastname: van Ewijk
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Sabina
authors.initials: S
authors.lastname: Paglialunga
authors.affiliation: Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.|CARIM-Cardiovascular Research Institute Maastricht, Maastricht, The Netherlands.
authors.forename: M Eline
authors.initials: ME
authors.lastname: Kooi
authors.affiliation: Department of Radiology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Nunes
authors.affiliation: NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.|Department of Human Movement Sciences, Maastricht University, Maastricht, The Netherlands.
authors.forename: Anne
authors.initials: A
authors.lastname: Gemmink
authors.affiliation: Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.
authors.forename: Jos
authors.initials: J
authors.lastname: Slenter
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Esther
authors.initials: E
authors.lastname: Kornips
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Johanna A
authors.initials: JA
authors.lastname: Jörgensen
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Joris
authors.initials: J
authors.lastname: Hoeks
authors.affiliation: Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.|CARIM-Cardiovascular Research Institute Maastricht, Maastricht, The Netherlands.
authors.forename: Joachim E
authors.initials: JE
authors.lastname: Wildberger
authors.affiliation: NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.|Department of Human Movement Sciences, Maastricht University, Maastricht, The Netherlands.
authors.forename: Matthijs K C
authors.initials: MK
authors.lastname: Hesselink
authors.affiliation: CARIM-Cardiovascular Research Institute Maastricht, Maastricht, The Netherlands.|Department of Molecular Genetics, Maastricht University, Maastricht, The Netherlands.
authors.forename: Jan F C
authors.initials: JF
authors.lastname: Glatz
authors.affiliation: Department of Radiology, Radboud University Medical Centre, Nijmegen, The Netherlands.
authors.forename: Arend
authors.initials: A
authors.lastname: Heerschap
authors.affiliation: Department of Nutrition, Metabolism and Genomics Groups, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands.|Netherlands Nutrigenomics Centre, Top Institute Food and Nutrition, Wageningen, The Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: Kersten
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Patrick
authors.initials: P
authors.lastname: Schrauwen
authors.affiliation: Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.|NUTRIM-School for Nutrition, Toxicology and Metabolism, Maastricht, The Netherlands.
authors.forename: Vera B
authors.initials: VB
authors.lastname: Schrauwen-Hinderling
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects; D004041:Dietary Fats / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism / Q000187:drug effects*; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D019518:Postprandial Period / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids