title: Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei.
abstract: CONCLUSIONS
  Rphq2, a minor gene for partial resistance to Puccinia hordei , was physically mapped in a 188 kbp introgression with suppressed recombination between haplotypes of rphq2 and Rphq2 barley cultivars.
  Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from cultivar Vada and line SusPtrit. Vada is immune to non-adapted Puccinia rust fungi, and partially resistant to P. hordei. SusPtrit is susceptible to several non-adapted rust fungi, and has been used for mapping QTLs for non-host and partial resistance. The BAC libraries help to identify genes determining the natural variation for partial and non-host resistances of barley to rust fungi. A major-effect QTL, Rphq2, for partial resistance to P. hordei was mapped in a complete Vada and an incomplete SusPtrit contig. The physical distance between the markers flanking Rphq2 was 195 Kbp in Vada and at least 226 Kbp in SusPtrit. This marker interval was predicted to contain 12 genes in either accession, of which only five genes were in common. The haplotypes represented by Vada and SusPtrit were found in 57 and 43%, respectively, of a 194 barley accessions panel. The lack of homology between the two haplotypes probably explains the suppression of recombination in the Rphq2 area and limit further genetic resolution in fine mapping. The possible candidate genes for Rphq2 encode peroxidases, kinases and a member of seven-in-absentia protein family. This result suggests that Rphq2 does not belong to the NB-LRR gene family and does not resemble any of the partial resistance genes cloned previously.
authors.affiliation: Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB, 6700 AJ, Wageningen, The Netherlands.|Department of Plant Science and Environmental Ecology, Faculty of Resource Science and Technology, University Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia.
authors.forename: F K S
authors.initials: FK
authors.lastname: Yeo
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing, 100093, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB, 6700 AJ, Wageningen, The Netherlands.|The Institute of Botany of the Academy of Science of the Czech Republic, Zámek 1, 252 43, Průhonice, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Vozabova
authors.affiliation: INRA, UMR1165, Unité de Recherche en Génomique Végétale, 91057, Evry, France.|Université d'Evry Val d'Essonne, UMR1165, Unité de Recherche en Génomique Végétale, 91057, Evry, France.
authors.forename: C
authors.initials: C
authors.lastname: Huneau
authors.affiliation: INRA, UMR1095, Genetics Diversity and Ecophysiology of Cereals, 63039, Clermont-Ferrand, France.|Université Blaise Pascal, UMR1095, Genetics Diversity and Ecophysiology of Cereals, 63039, Clermont-Ferrand, France.
authors.forename: P
authors.initials: P
authors.lastname: Leroy
authors.affiliation: INRA, UMR1165, Unité de Recherche en Génomique Végétale, 91057, Evry, France.|Université d'Evry Val d'Essonne, UMR1165, Unité de Recherche en Génomique Végétale, 91057, Evry, France.
authors.forename: B
authors.initials: B
authors.lastname: Chalhoub
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Fragrant Hill, Beijing, 100093, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Qi
authors.affiliation: Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB, 6700 AJ, Wageningen, The Netherlands. rients.niks@wur.nl.
authors.forename: R E
authors.initials: RE
authors.lastname: Niks
authors.affiliation: Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB, 6700 AJ, Wageningen, The Netherlands.|INRA, UMR1290, BIOGER, 78850, Thiverval-Grignon, France.|AgroParisTech, UMR1290, BIOGER, 78850, Thiverval-Grignon, France.
authors.forename: T C
authors.initials: TC
authors.lastname: Marcel
mesh_terms: D001487:Basidiomycota; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D018744:DNA, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant; D006239:Haplotypes; D001467:Hordeum / Q000235:genetics* / Q000382:microbiology; D058977:Molecular Sequence Annotation; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D059467:Transcriptome
chemical_list: D018744:DNA, Plant