title: Bioinspired anchoring AgNPs onto micro-nanoporous TiO2 orthopedic coatings: Trap-killing of bacteria, surface-regulated osteoblast functions and host responses.
abstract: The therapeutic applications of silver nanoparticles (AgNPs) against biomedical device-associated infections (BAI), by local delivery, are encountered with risks of detachment, instability and nanotoxicity in physiological milieus. To firmly anchor AgNPs onto modified biomaterial surfaces through tight physicochemical interactions would potentially relieve these concerns. Herein, we present a strategy for hierarchical TiO2/Ag coating, in an attempt to endow medical titanium (Ti) with anticorrosion and antibacterial properties whilst maintaining normal biological functions. In brief, by harnessing the adhesion and reactivity of bioinspired polydopamine, silver nanoparticles were easily immobilized onto peripheral surface and incorporated into interior cavity of a micro/nanoporous TiO2 ceramic coating in situ grown from template Ti. The resulting coating protected the substrate well from corrosion and gave a sustained release of Ag(+) up to 28 d. An interesting germicidal effect, termed "trap-killing", was observed against Staphylococcus aureus strain. The multiple osteoblast responses, i.e. adherence, spreading, proliferation, and differentiation, were retained normal or promoted, via a putative surface-initiated self-regulation mechanism. After subcutaneous implantation for a month, the coated specimens elicited minimal, comparable inflammatory responses relative to the control. Moreover, this simple and safe functionalization strategy manifested a good degree of flexibility towards three-dimensional sophisticated objects. Expectedly, it can become a prospective bench to bedside solution to current challenges facing orthopedics.
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiu
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Xuchen
authors.initials: X
authors.lastname: Xu
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: chengyan@pku.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100871, China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
authors.forename: Tingfei
authors.initials: T
authors.lastname: Xi
authors.affiliation: Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003343:Corrosion; D055664:Electrochemical Techniques; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D050296:Microbial Viability / Q000187:drug effects*; D009985:Orthopedics; D010006:Osteoblasts / Q000166:cytology*; D010084:Oxidation-Reduction; D016062:Porosity; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012834:Silver / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure; D040521:Subcutaneous Tissue / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D012834:Silver; D014025:Titanium
keywords: Antibacterial coatings; Micro-nano/porous; Poly(dopamine); Silver nanoparticles (AgNPs); TiO(2)/Ag coatings