title: SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy.
abstract: Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.
authors.forename: Graeme
authors.initials: G
authors.lastname: Ball
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.
authors.forename: Justin
authors.initials: J
authors.lastname: Demmerle
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.|Division of Structural Biology, Wellcome Trust Centre for Human Genetics University of Oxford, Oxford, UK.
authors.forename: Rainer
authors.initials: R
authors.lastname: Kaufmann
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.
authors.forename: Ilan
authors.initials: I
authors.lastname: Davis
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.
authors.forename: Ian M
authors.initials: IM
authors.lastname: Dobbie
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford, UK.
authors.forename: Lothar
authors.initials: L
authors.lastname: Schermelleh
mesh_terms: D000465:Algorithms; D002138:Calibration; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008029:Lighting / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D015203:Reproducibility of Results