title: Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9).
abstract: Avian influenza A viruses are serious veterinary pathogens that normally circulate among avian populations, causing substantial economic impacts. Some strains of avian influenza A viruses, such as H5N1, H9N2, and recently reported H7N9, have been occasionally found to adapt to humans from other species. In order to replicate efficiently in the new host, influenza viruses have to interact with a variety of host factors. In the present study, H7N9 nucleoprotein was transfected into human HEK293T cells, followed by immunoprecipitated and analyzed by proteomics approaches. A series of host proteins co-immunoprecipitated were identified with high confidence, some of which were found to be acetylated at their lysine residues. Bioinformatics analysis revealed that spliceosome might be the most relevant pathway involved in host response to nucleoprotein expression, increasing our emerging knowledge of host proteins that might be involved in influenza virus replication activities.
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. sunnn13@mails.jlu.edu.cn.
authors.forename: Ningning
authors.initials: N
authors.lastname: Sun
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China. Wanchunsun@jlu.edu.cn.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Shenzhen University, Shenzhen 518057, China. shuimingli@szu.edu.cn.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. yang.jb@live.cn.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Yang
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. yanglongfei@jlu.edu.cn.
authors.forename: Longfei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. qgh0521@jlu.edu.cn.
authors.forename: Guihua
authors.initials: G
authors.lastname: Quan
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. gaoxiang13@mails.jlu.edu.cn.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. jiuwzj1989@sina.com.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. chengxin14@mails.jlu.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. lizh7013@mails.jlu.edu.cn.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China. pengqs@jlu.edu.cn.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
authors.affiliation: Central Laboratory, Jilin University Second Hospital, Changchun 130041, China. liu_ning@jlu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
mesh_terms: D000107:Acetylation; D002352:Carrier Proteins / Q000378:metabolism*; D019295:Computational Biology; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000378:metabolism*; D007251:Influenza, Human / Q000378:metabolism* / Q000821:virology*; D008239:Lysine; D019590:Nucleocapsid Proteins; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D016601:RNA-Binding Proteins / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014758:Viral Core Proteins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D008239:Lysine
keywords: co-immunoprecipitation; influenza A virus; lysine acetylation; mass spectrometry; nucleoprotein