title: A Novel Saccharomyces cerevisiae FG Nucleoporin Mutant Collection for Use in Nuclear Pore Complex Functional Experiments.
abstract: FG nucleoporins (Nups) are the class of proteins that both generate the permeability barrier and mediate selective transport through the nuclear pore complex (NPC). The FG Nup family has 11 members in Saccharomyces cerevisiae, and the study of mutants lacking different FG domains has been instrumental in testing transport models. To continue analyzing the distinct functional roles of FG Nups in vivo, additional robust genetic tools are required. Here, we describe a novel collection of S. cerevisiae mutant strains in which the FG domains of different groups of Nups are absent (Δ) in the greatest number documented to date. Using this plasmid-based ΔFG strategy, we find that a GLFG domain-only pore is sufficient for viability. The resulting extensive plasmid and strain resources are available to the scientific community for future in-depth in vivo studies of NPC transport.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37240-7935.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Adams
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37240-7935.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Terry
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37240-7935 susan.wente@vanderbilt.edu.
authors.forename: Susan R
authors.initials: SR
authors.lastname: Wente
mesh_terms: D015870:Gene Expression; D056726:Genetic Association Studies; D009154:Mutation; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D017384:Sequence Deletion
chemical_list: D028861:Nuclear Pore Complex Proteins
keywords: FG nucleoporin; S. cerevisiae; nuclear pore complex