title: Amylose-Based Cationic Star Polymers for siRNA Delivery.
abstract: A new siRNA delivery system using a cationic glyco-star polymer is described. Spermine-modified 8-arm amylose star polymer (with a degree of polymerization of approximately 60 per arm) was synthesized by chemoenzymatic methods. The cationic star polymer effectively bound to siRNA and formed spherical complexes with an average hydrodynamic diameter of 230 nm. The cationic 8-arm star polymer complexes showed superior cellular uptake characteristics and higher gene silencing effects than a cationic 1-arm polymer. These results suggest that amylose-based star polymers are a promising nanoplatform for glycobiomaterials.
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Nishimura
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Umezaki
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.
authors.forename: Sada-atsu
authors.initials: SA
authors.lastname: Mukai
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Sawada
authors.affiliation: Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8150, Japan ; ERATO Akiyoshi Bio-Nanotransporter Project, JST, Katsura, Nishikyo, Kyoto 615-8150, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D000688:Amylose / Q000737:chemistry*; D000818:Animals; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D051379:Mice; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D002412:Cations; D004337:Drug Carriers; D011108:Polymers; D034741:RNA, Small Interfering; D000688:Amylose