title: Interferon induction by avian reovirus.
abstract: We have previously shown that the replication of avian reovirus (ARV) in chicken embryo fibroblasts (CEF) is more resistant to the antiviral action of interferon (IFN) than the replication of vesicular stomatitis virus (VSV) or vaccinia virus (VV). In this study we examined the capacity of these three viruses to induce the expression of IFN when infecting avian cells. Efficient expression of both type-α and type-β IFNs, as well as of the double-stranded RNA (dsRNA)-activated protein kinase (PKR), takes place in ARV-infected CEF, but not in cells infected with VSV or VV. PKR expression is not directly induced by ARV infection, but by the IFN secreted by ARV-infected cells. IFN induction in ARV-infected cells requires viral uncoating, but not viral gene expression, a situation similar to that reported for apoptosis induction by ARV-infected cells. However, our results demonstrate that IFN induction by ARV-infected CEF occurs by a caspase-independent mechanism.
authors.affiliation: Centro de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Lostalé-Seijo
authors.affiliation: Centro de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Martínez-Costas
authors.affiliation: Centro de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, Spain. Electronic address: franciscojavier.benavente@usc.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Benavente
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D006224:Cricetinae; D016898:Interferon-alpha / Q000096:biosynthesis / Q000276:immunology*; D016899:Interferon-beta / Q000096:biosynthesis / Q000276:immunology*; D030101:Orthoreovirus, Avian / Q000254:growth & development / Q000276:immunology*; D061251:Primary Cell Culture; D014616:Vaccinia virus / Q000254:growth & development / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development / Q000276:immunology*; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta; D019892:eIF-2 Kinase; D020169:Caspases
keywords: Apoptosis; Avian reovirus; Interferon induction; PKR; Virus uncoating