title: Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26.
abstract: Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.
authors.affiliation: School of Marine Sciences, Key Laboratory of Applied Marine Biotechnology (Ministry of Education), Ningbo University, Ningbo, 315211, China.|Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Wei
authors.affiliation: School of Marine Sciences, Key Laboratory of Applied Marine Biotechnology (Ministry of Education), Ningbo University, Ningbo, 315211, China. hahaliang408@126.com.
authors.forename: Lianliang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Guo
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Bao-chao
authors.initials: BC
authors.lastname: Hou
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Qiu-ling
authors.initials: QL
authors.lastname: Fan
authors.affiliation: Zhejiang Provincial Key Laboratory of Food Biotechnology, Institute of Bioengineering, Hangzhou Wahaha Group Co., Ltd., Hangzhou, 310018, China.
authors.forename: Kai-xiang
authors.initials: KX
authors.lastname: Wang
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Yingdi
authors.initials: Y
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Jian-jiang
authors.initials: JJ
authors.lastname: Zhong
mesh_terms: D018533:Biomass; D019149:Bioreactors; D062610:Fungal Polysaccharides / Q000096:biosynthesis*; D020940:Reishi / Q000254:growth & development*; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D062610:Fungal Polysaccharides; D014315:Triterpenes; C556862:ganoderic acid
keywords: Fed-batch; Fermentation; Ganoderic acids; Ganoderma; Polysaccharide