title: Structure of an Amino Acid-Decorated Exopolysaccharide Secreted by a Vibrio alginolyticus Strain.
abstract: Vibrio alginolyticus (CNCM I-4994) secretes an exopolysaccharide that can be used as an ingredient in cosmetic applications. The structure was resolved using chromatography and one- and two-dimensional NMR spectroscopy experiments. The results show that the carbohydrate backbone is made of two residues: d-galacturonic acid and N-acetyl-d-glucosamine (GlcNac), which together constitute a tetrasaccharide repetition unit: [→3)-α-d-GalA-(1→4)-α-d-GalA-(1→3)-α-d-GalA-(1→3)-β-GlcNAc(1→]. Two amino acids, alanine and serine, are linked to GalA residues via amido linkages. The position and the distribution of the amino acids were characterized by two-dimensional NMR spectroscopy. To our knowledge, this is the first description of a structure for a marine exopolysaccharide decorated with an amino acid.
authors.affiliation: Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France. sophie.drouillard@cermav.cnrs.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Drouillard
authors.affiliation: Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France. isabelle.jeacomine@cermav.cnrs.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jeacomine
authors.affiliation: Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France. laurine.buon.drouillard@cermav.cnrs.fr.
authors.forename: Laurine
authors.initials: L
authors.lastname: Buon
authors.affiliation: Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France. claire.boisset-helbert@cermav.cnrs.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Boisset
authors.affiliation: POLYMARIS BIOTECHNOLOGY, Aéropôle Centre, 29600 Morlaix, France. Anthony.courtois@polymaris.com.
authors.forename: Anthony
authors.initials: A
authors.lastname: Courtois
authors.affiliation: POLYMARIS BIOTECHNOLOGY, Aéropôle Centre, 29600 Morlaix, France. Bertrand.thollas@polymaris.com.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Thollas
authors.affiliation: CODIF INTERNATIONAL, 35610 Roz-sur-Couesnon, France. py.morvan@codif.com.
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Morvan
authors.affiliation: CODIF INTERNATIONAL, 35610 Roz-sur-Couesnon, France. r.vallee@codif.com.
authors.forename: Romuald
authors.initials: R
authors.lastname: Vallée
authors.affiliation: Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France. william.helbert@cermav.cnrs.fr.
authors.forename: William
authors.initials: W
authors.lastname: Helbert
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D044149:Vibrio alginolyticus / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011135:Polysaccharides, Bacterial
keywords: Vibrio alginolyticus; amino acids; exopolysaccharide; ingredient