title: Coumarins with α-glucosidase and α-amylase inhibitory activities from the flower of Edgeworthia gardneri.
abstract: The flower of Edgeworthia gardneri is consumed in beverages in Tibet and has potential health benefits for diabetes. As a part of our continuous studies on dietary supplements for diabetes, two monomers, five dimers and one trimer of coumarins were isolated from the flowers of E. gardneri. One dimer was a new compound (1) and its structure was determined by spectroscopic methods, including multiple NMR techniques and mass spectrometry. The inhibitory activities of all coumarins against α-amylase and α-glucosidase were evaluated. Compound 4 displayed potent inhibitory effect on both α-amylase and α-glucosidase, with an IC50 of 90 and 86μg/mL, respectively. The IC50 of compound 3 against α-glucosidase was 18.7μg/mL, and its inhibition mode was noncompetitive. Based on the fluorescence analysis, the binding constant and the number of binding sites of compound 3 were calculated as 2.05×10(5) and 1.24, respectively. Furthermore, the interaction between compound 3 and α-glucosidase was a spontaneous process that was driven mainly by hydrophobic force. This study could facilitate the utilization of E gardneri as functional food ingredient.
authors.affiliation: Laboratory of Natural Medicinal Chemistry & Green Chemistry, Faculty of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
authors.forename: Deng-Gao
authors.initials: DG
authors.lastname: Zhao
authors.affiliation: Laboratory of Natural Medicinal Chemistry & Green Chemistry, Faculty of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
authors.forename: Ai-Yu
authors.initials: AY
authors.lastname: Zhou
authors.affiliation: Laboratory of Natural Medicinal Chemistry & Green Chemistry, Faculty of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Du
authors.affiliation: School of Chemistry and Environment Engineering, Wuyi University, Jiangmen 529020, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Natural Medicinal Chemistry & Green Chemistry, Faculty of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China; School of Chemistry and Environment Engineering, Wuyi University, Jiangmen 529020, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Laboratory of Natural Medicinal Chemistry & Green Chemistry, Faculty of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China. Electronic address: mayy14@mail2.gdut.edu.cn.
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Ma
mesh_terms: D003374:Coumarins / Q000737:chemistry* / Q000302:isolation & purification; D035264:Flowers / Q000737:chemistry*; D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D029645:Thymelaeaceae / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D003374:Coumarins; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D000516:alpha-Amylases; D000520:alpha-Glucosidases
keywords: Coumarins; Edgeworthia gardneri; Herbal tea; α-Amylase inhibition; α-Glucosidase inhibition