title: Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers.
abstract: A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. China.|Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.affiliation: Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Hou
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. China.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D012911:Snakes / Q000235:genetics*
chemical_list: D002412:Cations; D017931:DNA Primers; D005456:Fluorescent Dyes; D011108:Polymers; D004247:DNA