title: Whole genome sequence to decipher the resistome of Shewanella algae, a multidrug-resistant bacterium responsible for pneumonia, Marseille, France.
abstract: We characterize and decipher the resistome and the virulence factors of Shewanella algae MARS 14, a multidrug-resistant clinical strain using the whole genome sequencing (WGS) strategy. The bacteria were isolated from the bronchoalveolar lavage of a hospitalized patient in the Timone Hospital in Marseille, France who developed pneumonia after plunging into the Mediterranean Sea.
  
  
  RESULTS
  The genome size of S. algae MARS 14 was 5,005,710 bp with 52.8% guanine cytosine content. The resistome includes members of class C and D beta-lactamases and numerous multidrug-efflux pumps. We also found the presence of several hemolysins genes, a complete flagellum system gene cluster and genes responsible for biofilm formation. Moreover, we reported for the first time in a clinical strain of Shewanella spp. the presence of a bacteriocin (marinocin).
  
  
  CONCLUSIONS
  The WGS analysis of this pathogen provides insight into its virulence factors and resistance to antibiotics.
authors.affiliation: a URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, Méditerranée Infection, Facultés de Médecine et de Pharmacie , Aix-Marseille Université , Marseille Cedex 05 , France.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cimmino
authors.affiliation: a URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, Méditerranée Infection, Facultés de Médecine et de Pharmacie , Aix-Marseille Université , Marseille Cedex 05 , France.
authors.forename: Abiola Olumuyiwa
authors.initials: AO
authors.lastname: Olaitan
authors.affiliation: a URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, Méditerranée Infection, Facultés de Médecine et de Pharmacie , Aix-Marseille Université , Marseille Cedex 05 , France.|b Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie , Centre Hospitalo-Universitaire Timone, Méditerranée Infection , Marseille Cedex 05 , France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Rolain
mesh_terms: D000328:Adult; D001430:Bacteriocins / Q000235:genetics; D018441:Biofilms; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005407:Flagella / Q000235:genetics; D005602:France; D019450:Genes, MDR / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008297:Male; D008522:Mediterranean Sea; D011015:Pneumonia, Aspiration / Q000382:microbiology*; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D020592:Shewanella / Q000235:genetics* / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001430:Bacteriocins; D006460:Hemolysin Proteins; D037521:Virulence Factors; D001618:beta-Lactamases
keywords: Antibiotic resistance; bacteriocin; genome; virulence genes