title: HTP-OligoDesigner: An Online Primer Design Tool for High-Throughput Gene Cloning and Site-Directed Mutagenesis.
abstract: Following burgeoning genomic and transcriptomic sequencing data, biochemical and molecular biology groups worldwide are implementing high-throughput cloning and mutagenesis facilities in order to obtain a large number of soluble proteins for structural and functional characterization. Since manual primer design can be a time-consuming and error-generating step, particularly when working with hundreds of targets, the automation of primer design process becomes highly desirable. HTP-OligoDesigner was created to provide the scientific community with a simple and intuitive online primer design tool for both laboratory-scale and high-throughput projects of sequence-independent gene cloning and site-directed mutagenesis and a Tm calculator for quick queries.
authors.affiliation: 1 Grupo de Biotecnologia Molecular, Instituto de Física de São Carlos, Universidade de São Paulo , São Carlos, São Paulo, Brazil .
authors.forename: Cesar M
authors.initials: CM
authors.lastname: Camilo
authors.affiliation: 2 Laboratório de Química Medicinal e Computacional, Centro de Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo , São Carlos, São Paulo, Brazil .
authors.forename: Gustavo M A
authors.initials: GM
authors.lastname: Lima
authors.affiliation: 2 Laboratório de Química Medicinal e Computacional, Centro de Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo , São Carlos, São Paulo, Brazil .
authors.forename: Fernando V
authors.initials: FV
authors.lastname: Maluf
authors.affiliation: 2 Laboratório de Química Medicinal e Computacional, Centro de Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo , São Carlos, São Paulo, Brazil .
authors.forename: Rafael V C
authors.initials: RV
authors.lastname: Guido
authors.affiliation: 1 Grupo de Biotecnologia Molecular, Instituto de Física de São Carlos, Universidade de São Paulo , São Carlos, São Paulo, Brazil .
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D012984:Software
chemical_list: D017931:DNA Primers
keywords: gene cloning; high throughput; primer design tool; site-directed mutagenesis