title: Molecular identification of Echinococcus granulosus on the Tibetan Plateau using mitochondrial DNA markers.
abstract: Cystic echinococcosis (CE) is an important worldwide zoonotic disease that causes large economic losses and human suffering. Echinococcus granulosus, the causative agent of CE, exhibits different genotypes in different locations. In order to identify its genotypes and analyze its genetic structure on the Tibetan Plateau, we collected 72 hydatid cysts from different intermediate hosts and amplified and sequenced their mitochondrial cytochrome c oxidase subunit 2 (cox2) genes. Seventy isolates were identified as the E. granulosus G1 genotype, while two isolates belonged to the G6 genotype. There were 18 haplotypes among the 70 E. granulosus isolates, which exhibited a star-like network pattern and shared a common haplotype (H1). There was little difference between geographical sub-populations. Our results suggest that a recent E. granulosus population expansion occurred on the Tibetan Plateau, suggesting that E. granulosus was introduced into China. This study increases the basic molecular data needed for the molecular diagnosis, epidemiology, prevention, and control of Echinococcus diseases.
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: D
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: X
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: N
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.affiliation: Ganzi Animal Science Institute, Kangding, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Tibetan Center for Animal Disease Control and Preventive, Lasa, China.
authors.forename: T
authors.initials: T
authors.lastname: Dawa
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China.
authors.forename: X
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.
authors.forename: G
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004443:Echinococcosis / Q000469:parasitology*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics*; D019143:Evolution, Molecular; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D010802:Phylogeny; D011110:Polymorphism, Genetic; D018609:Tibet
chemical_list: D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D005819:Genetic Markers