title: Antitumor activity of a novel survivin siRNA.
abstract: Breast cancer resistance to therapy can result from expression of antiapoptotic genes. Survivin is an antiapoptotic gene that is over expressed in most human tumors. RNA interference using short interfering RNA (siRNA) can be used to specifically inhibit survivin expression. A novel siRNA targeting survivin was used to process MCF-7 cells. Cellular survivin mRNA and protein levels were determined by real-time qRT-PCR and Western blot, respectively. Cellular morphology and cell cycle were determined by fluorescence microscopy and flow cytometry. Cell proliferation was measured by MTT assay. Our data showed that the novel survivin-targeted siRNA could efficiently knockdown the expression of survivin, inhibit cell proliferation and cell cycle, especially at the G2/M checkpoint. These data suggest that the siRNA has potential for therapeutic applications.
authors.affiliation: Institute of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Li
authors.forename: Yujing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
authors.affiliation: Institute of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Division of Paediatrics, First Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Du
authors.affiliation: Second Hospital of Jilin University, Changchun, Jilin, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Lesheng
authors.initials: L
authors.lastname: Teng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000494:pharmacology*; D000077022:Survivin; D014162:Transfection; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; D051028:bcl-2-Associated X Protein; D053148:Caspase 3