title: Engineered T cell therapies.
abstract: Alongside advancements in gene therapy for inherited immune disorders, the need for effective alternative therapeutic options for other conditions has resulted in an expansion in the field of research for T cell gene therapy. T cells are easily obtained and can be induced to divide robustly ex vivo, a characteristic that allows them to be highly permissible to viral vector-mediated introduction of transgenes. Pioneering clinical trials targeting cancers and infectious diseases have provided safety and feasibility data and important information about persistence of engineered cells in vivo. Here, we review clinical experiences with γ-retroviral and lentiviral vectors and consider the potential of integrating transposon-based vectors as well as specific genome editing with designer nucleases in engineered T cell therapies.
authors.affiliation: Molecular Immunology Unit,Institute of Child Health (ICH),University College London (UCL),30 Guilford Street,London WC1N 1EH,UK.
authors.forename: Anne-Christine
authors.initials: AC
authors.lastname: Field
authors.affiliation: Molecular Immunology Unit,Institute of Child Health (ICH),University College London (UCL),30 Guilford Street,London WC1N 1EH,UK.
authors.forename: Waseem
authors.initials: W
authors.lastname: Qasim
mesh_terms: D060846:Cell Engineering / Q000379:methods; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002986:Clinical Trials as Topic; D004251:DNA Transposable Elements; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019076:Transgenes
chemical_list: D004251:DNA Transposable Elements; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins