title: Rapid identification of haloarchaea and methanoarchaea using the matrix assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: The aim of this study was to classify certain environmental haloarchaea and methanoarchaea using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and to expand the archaeal mass spectral database. A total of 69 archaea were collected including type strains and samples isolated locally from different environments. For extraction of the haloarchaeal total cell peptides/proteins, a simple method of acetonitrile extraction was developed. Cluster analysis conducted with the MALDI-TOF MS data overcame the high divergence in intragenomic 16S rRNA sequences in haloarchaea and clearly distinguished Methanohalophilus mahii from M. portucalensis. Putative biomarkers that can distinguish several particular archaeal genera were also assigned. In conclusion, this study expands the mass spectral database of peptide/protein fingerprints from bacteria and fungi to the archaea domain and provides a rapid identification platform for environmental archaeal samples.
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
authors.forename: Chao-Jen
authors.initials: CJ
authors.lastname: Shih
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;
authors.forename: Sheng-Chung
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;
authors.forename: Chieh-Yin
authors.initials: CY
authors.lastname: Weng
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan;|Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.|Ph.D. Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taiwan.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lai
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.|Ph.D. Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taiwan.
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Yang
mesh_terms: D001105:Archaea / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*