title: Enterovirus 71 induces autophagy by regulating has-miR-30a expression to promote viral replication.
abstract: Enterovirus 71 (EV71), the etiological agent of hand-foot-and-mouth disease, has increasingly become a public health challenge around the world. Previous studies reported that EV71 infection can induce autophagic machinery to enhance viral replication in vitro and in vivo, but did not address the underlying mechanisms. Increasing evidence suggests that autophagy, in a virus-specific manner, may function to degrade viruses or facilitate viral replication. In this study, we reported that EV71 infection of human epidermoid carcinoma (Hep2) and African green monkey kidney cells (Vero) induced autophagy, which is beneficial for viral replication. Our investigation of the mechanisms revealed that EV71 infection resulted in the reduction of cellular miR-30a, which led to the inhibition of Beclin-1, a key autophagy-promoting gene that plays important roles at the early phase of autophagosome formation. We provided further evidence that by modulating cellular miR-30a level through either overexpression or inhibition, one can inhibit or promote EV71 replication, respectively, through regulating autophagic activity.
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Deyan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Feng
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Nanjing Children's Hospital, Nanjing Medical University, Nanjing, PR China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Lv
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China; State Key Lab of Analytical Chemistry for Life Science, Nanjing University, Nanjing, PR China; Medical School and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Nanjing Children's Hospital, Nanjing Medical University, Nanjing, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Center for Public Health Research, Medical School, Nanjing University, Nanjing, PR China; State Key Lab of Analytical Chemistry for Life Science, Nanjing University, Nanjing, PR China; Medical School and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, PR China. Electronic address: wzhw@nju.edu.cn.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D001343:Autophagy / Q000502:physiology; D000071186:Beclin-1; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015536:Down-Regulation; D029821:Enterovirus A, Human / Q000502:physiology*; D004769:Enterovirus Infections / Q000235:genetics / Q000473:pathology / Q000628:therapy; D006801:Humans; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D035683:MicroRNAs / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D018613:Microscopy, Confocal; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; C546086:MIRN30b microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs
keywords: Autophagy; Beclin-1; Enterovirus 71; Viral replication; miR-30a