title: Anthocyanin composition and expression analysis of anthocyanin biosynthetic genes in kidney bean pod.
abstract: Kidney bean (Phaseolus vulgaris L.) is an important dietary legume crop cultivated and consumed worldwide. A purple cultivar (Zi Bawang) and a green cultivar (Chun Qiu), the main difference of which is in the pod skin color, were selected for the study. Malvidin 3, 5-diglucoside is identified as the major anthocyanin in the pod skin of Zi Bawang by HPLC-ESI-MS/MS. Three regulatory genes PvMYB1, PvMYB2, PvTT8-1 and most structural genes are dramatically up-regulated in purple pod skin compared to those in other materials. Significantly decreased expression levels of all regulatory genes and most biosynthetic genes are also detected in the purple skin of pods covered with bags compared to non-covered ones. All the results suggest that PvMYB1, PvMYB2 and PvTT8-1 might play a critical role in transcriptional activation of most anthocyanin biosynthetic genes in purple kidney bean pod.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Jingtao
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Baolu
authors.initials: B
authors.lastname: Cui
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Wencheng
authors.initials: W
authors.lastname: Yin
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, PR China. Electronic address: huzongli71@163.com.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics* / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant; D008027:Light; D027805:Phaseolus / Q000235:genetics*; D010641:Phenotype; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins
keywords: Anthocyanin composition; HPLC-ESI-MS/MS; Molecular regulation; Purple kidney bean