title: Sertraline reduces glutamate uptake in human platelets.
abstract: Mitochondrial damage and declines in ATP levels have been recently attributed to sertraline. The effects of sertraline on different parameters were investigated in washed platelets from 18 healthy male volunteers, after 24h of drug exposure. Sertraline toxicity was observed only at the highest concentrations, 30 and 100 μM, which significantly reduced platelet viability to 76 ± 3% and 20 ± 2%, respectively. The same concentrations significantly decreased total ATP to 73 ± 3% and 13 ± 2%, respectively. Basal values of glycogen were not significantly affected by sertraline treatment. Glutamate uptake was significantly reduced after treatment with 3, 30 and 100 μM, by 28 ± 6%, 32 ± 5% and 54 ± 4%, respectively. Our data showed that sertraline at therapeutic concentrations does not compromise platelet viability and ATP levels, but they suggest that in a situation where extracellular glutamate levels are potentially increased, sertraline might aggravate an excitotoxic condition.
authors.affiliation: Laboratory of Cellular Neurobiology, Department of Morphological Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Débora Olmedo
authors.initials: DO
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Cellular Biochemistry, Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Ivi Juliana
authors.initials: IJ
authors.lastname: Bristot
authors.affiliation: Laboratory of Cellular Biochemistry, Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fábio
authors.initials: F
authors.lastname: Klamt
authors.affiliation: Laboratory of Cellular Neurobiology, Department of Morphological Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: marcosfrizzo@ufrgs.br.
authors.forename: Marcos Emílio
authors.initials: ME
authors.lastname: Frizzo
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D018698:Glutamic Acid / Q000378:metabolism*; D006003:Glycogen / Q000097:blood / Q000378:metabolism; D006801:Humans; D008297:Male; D017367:Selective Serotonin Reuptake Inhibitors / Q000633:toxicity*; D020280:Sertraline / Q000633:toxicity*
chemical_list: D000255:Adenosine Triphosphate; D018698:Glutamic Acid; D006003:Glycogen; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline
keywords: Excitotoxicity; Lithium; SSRI; Seizures