title: Omentin-1 plasma levels and cholesterol metabolism in obese patients with diabetes mellitus type 1: impact of weight reduction.
abstract: BACKGROUND
  Omentin-1 is an anti-inflammatory adipokine produced preferentially by visceral adipose tissue. Plasma levels of omentin-1 are decreased in obesity and other insulin-resistant states. Insulin resistance contributes to the changes of cholesterol synthesis and absorption as well. The aim of this study was to characterise omentin-1 plasma levels in obese patients with diabetes mellitus type 1 during weight reduction, and to elucidate the relationship between cholesterol metabolism and omentin-1.
  
  
  METHODS
  Plasma levels of omentin-1 were measured in obese type 1 diabetics (n=14, body mass index >30 kg m(-2), age 29-62 years) by enzyme-linked immunosorbent assay (BioVendor). Gas chromatography with flame ionisation detector (Fisons Plc.,) was used to measure squalene and non-cholesterol sterols-markers of cholesterol synthesis and absorption (phase I). Measurements were repeated after 1 month (phase II; 1 week of fasting in the hospital setting and 3 weeks on a diet containing 150 g saccharides per day) and after 1 year (phase III) on a diet with 225 g saccharides per day.
  
  
  RESULTS
  Omentin-1 plasma levels were stable during phases I and II, but significantly increased (P<0.001) during phase III. Omentin-1 plasma dynamics were significantly associated with plasma levels of high-density lipoprotein (P=0.005) and triacylglycerols (P=0.01), as well as with lathosterol (P=0.03).
  
  
  CONCLUSIONS
  Omentin-1 plasma levels significantly increased during the weight reduction programme. Omentin-1 plasma dynamics suggest a close relationship with cholesterol metabolism.
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Centre for Research and Development, University Hospital in Hradec Králové, Hradec Králové, Czech Republic.|Department of Clinical Biochemistry, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Medical Faculty in Hradec Králové, Charles University in Prague, Hradec Králové1, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Lesná
authors.affiliation: Centre for Research and Development, University Hospital in Hradec Králové, Hradec Králové, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Tichá
authors.affiliation: Centre for Research and Development, University Hospital in Hradec Králové, Hradec Králové, Czech Republic.|Department of Clinical Biochemistry, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Hyšpler
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Musil
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Centre for Research and Development, University Hospital in Hradec Králové, Hradec Králové, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Bláha
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Medical Faculty in Hradec Králové, Charles University in Prague, Hradec Králové1, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Sobotka
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Centre for Research and Development, University Hospital in Hradec Králové, Hradec Králové, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Zadák
authors.affiliation: 3rd Department of Internal Medicine, University Hospital in Hradec Králové, Hradec Králové5, Czech Republic.|Medical Faculty in Hradec Králové, Charles University in Prague, Hradec Králové1, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Šmahelová