title: Monitoring the dynamics of emergence of a non-canonical recombinant of Tomato yellow leaf curl virus and displacement of its parental viruses in tomato.
abstract: Recombinant viruses are increasingly being reported but the dynamics of their emergence is rarely documented. A new recombinant Tomato yellow leaf curl virus (TYLCV-IS76) was detected for the first time in 2010 in Southern Morocco (Souss). An original diagnostic tool was needed to fit its unusual recombination profile. Although IS76 was detected following the appearance of Tylc symptoms on tolerant tomato plants, symptoms could not be associated to IS76 or to a synergy with criniviruses. According to infection profiles of Tylc-associated viruses determined on 879 plant samples collected between 1998 and 2014 and a Bayesian inference applied to genomic sequences of representatives of TYLCV, IS76 emerged in Southern Morocco at the end of the 1990s, replaced the parental viruses between 2004 and 2012 in Souss and is spreading towards the North of Morocco. The emergence of IS76 coincides with the increasing use of tolerant cultivars in the 2000s.
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France; Ecole Nationale d׳Agriculture de Meknès, BPS 40, Meknès, Morocco; Faculté des Sciences de Meknès, BP 11201, Avenue Zitoune, Meknès, Morocco.
authors.forename: Z
authors.initials: Z
authors.lastname: Belabess
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France.
authors.forename: S
authors.initials: S
authors.lastname: Dallot
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France; Ecole Nationale d׳Agriculture de Meknès, BPS 40, Meknès, Morocco; Faculté des Sciences de Meknès, BP 11201, Avenue Zitoune, Meknès, Morocco.
authors.forename: S
authors.initials: S
authors.lastname: El-Montaser
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France.
authors.forename: M
authors.initials: M
authors.lastname: Granier
authors.affiliation: Gautier Semences, Route d׳Avignon, 13630 Eyragues, France.
authors.forename: M
authors.initials: M
authors.lastname: Majde
authors.affiliation: Ecole Nationale d׳Agriculture de Meknès, BPS 40, Meknès, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Tahiri
authors.affiliation: Faculté des Sciences de Meknès, BP 11201, Avenue Zitoune, Meknès, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Blenzar
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France.
authors.forename: C
authors.initials: C
authors.lastname: Urbino
authors.affiliation: CIRAD-INRA-SupAgro, UMR BGPI, CIRAD, TA A -54/K, Campus International de Baillarguet, F-34398 Montpellier Cedex 5, France. Electronic address: michel.peterschmitt@cirad.fr.
authors.forename: M
authors.initials: M
authors.lastname: Peterschmitt
mesh_terms: D001483:Base Sequence; D001499:Bayes Theorem; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D011995:Recombination, Genetic; D012526:Satellite Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: BEAST; Bean; Crinivirus; Diagnostic; Morocco; Multiplex PCR; TYLCMalV; ToCV; Tolerance gene Ty1