title: Detection and characterization of potentially pathogenic Aeromonas sobria isolated from fish Hypophthalmichthys molitrix (Cypriniformes: Cyprinidae).
abstract: The current study focuses on the detection and characterization of potentially pathogenic Aeromonas sobria from fish silver carp (Hypophthalmichthys molitrix). Assessment of clinical, microbiological, pathological and biochemical characteristics of A. sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. Clinically the infected fish (H. molitrix) was observed for various types of symptoms. A total of 33 colonies of A. sobria strain were isolated from 20 cultured H. molitrix, collected from controlled fish pond. Microscopic examination revealed that the strains were rod-shaped, Gram negative bacteria. The revealed percent probability identification of A. sobria from the biochemical characterization in VITEK system was 93% with gram negative (GN) card. The histopathology of Gills caused by this bacterium, A. sobria indicate haemorrhagic gill epithelia and epithelial hyperplasia. Lamelar epithelial hypertrophy and hyperplasia with degenerative changes of the epithelium and hypertrophic epitheliocystis infected cells on gills of H. molitrix were observed during the present study.
authors.affiliation: Department of Environmental Science, University of Kashmir, Srinagar 190006, India; Centre of Research for Development, University of Kashmir, Srinagar 190006, India. Electronic address: dargowharhamid@gmail.com.
authors.forename: Gowhar H
authors.initials: GH
authors.lastname: Dar
authors.affiliation: Department of Zoology and Environmental Sciences, Punjabi University, Patialia 147002, India.
authors.forename: Shoaib A
authors.initials: SA
authors.lastname: Dar
authors.affiliation: Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
authors.forename: Azra N
authors.initials: AN
authors.lastname: Kamili
authors.affiliation: Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
authors.forename: Mohammad Z
authors.initials: MZ
authors.lastname: Chishti
authors.affiliation: Department of Zoology, University of Kashmir, Srinagar 190006, India.
authors.forename: Fayaz
authors.initials: F
authors.lastname: Ahmad
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D002347:Carps / Q000382:microbiology*; D005393:Fish Diseases / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D014774:Virulence
keywords: Aeromonas sobria; Exotic carp; Hypophthalmichthys molitrix; VITEK-2 system