title: Dietary extra virgin olive oil attenuates kidney injury in pristane-induced SLE model via activation of HO-1/Nrf-2 antioxidant pathway and suppression of JAK/STAT, NF-κB and MAPK activation.
abstract: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by a widespread organ involvement. Recent studies have suggested that extra virgin olive oil (EVOO) might possess preventive effects on this immunoinflammation-related disease. However, its role in SLE remained unknown. In this work, we evaluated the effects of EVOO diet in a pristane-induced SLE model in mice. Three-month-old mice received an injection of pristane or saline solution and were fed with different experimental diets: sunflower oil diet or EVOO diet. After 24weeks, mice were sacrificed, spleens were collected and kidneys were removed for immunoinflammatory detections. The kidney expression of microsomal prostaglandin E synthase 1, heme oxygenase 1 (HO-1), nuclear factor E2-related factor 2 (Nrf-2), mitogen-activated protein kinases (MAPKs), Janus kinase/signal transducer and activator of transcription (JAK/STAT) and nuclear transcription factor-kappa B (NF-κB) pathways were studied by western blotting. In addition to macroscopic and histological analyses, serum matrix metalloproteinase 3 (MMP-3) levels and proinflammatory cytokines production in splenocytes were evaluated by enzyme-linked immunoassay. We have demonstrated that EVOO diet significantly reduced renal damage and decreased MMP-3 serum and PGE2 kidney levels as well as the proinflammatory cytokines production in splenocytes. Our data indicate that Nrf-2 and HO-1 protein expressions were up-regulated in those mice fed with EVOO and the activation of JAK/STAT, MAPK and NF-κB pathways were drastically ameliorated. These results support the interest of EVOO as a beneficial functional food exerting a preventive/palliative role in the management of SLE.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Aparicio-Soto
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Sánchez-Hidalgo
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Cárdeno
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain.
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Rosillo
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Sánchez-Fidalgo
authors.affiliation: Department of Cytology and Histology, School of Medicine, University of Seville, 41004 Sevilla, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Utrilla
authors.affiliation: Department of Cytology and Histology, School of Medicine, University of Seville, 41004 Sevilla, Spain.
authors.forename: Inés
authors.initials: I
authors.lastname: Martín-Lacave
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Seville, 41004 Sevilla, Spain. Electronic address: calarcon@us.es.
authors.forename: Catalina
authors.initials: C
authors.lastname: Alarcón-de-la-Lastra
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D007674:Kidney Diseases / Q000209:etiology*; D008180:Lupus Erythematosus, Systemic / Q000150:complications*; D020935:MAP Kinase Signaling System; D051379:Mice; D008807:Mice, Inbred BALB C; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D000069463:Olive Oil / Q000008:administration & dosage*; D000071876:Prostaglandin-E Synthases
chemical_list: D000975:Antioxidants; D016207:Cytokines; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495636:Nfe2l2 protein, mouse; D000069463:Olive Oil; D051547:Heme Oxygenase-1; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases
keywords: EVOO; Immunomodulation; Inflammation; Polyphenols; SLE