title: Ack1 overexpression promotes metastasis and indicates poor prognosis of hepatocellular carcinoma.
abstract: Despite the substantial data supporting the oncogenic role of Ack1, the predictive value and biologic role of Ack1 in hepatocellular carcinoma (HCC) metastasis remains unknown. In this study, both correlations of Ack1 expression with prognosis of HCC, and the role of Ack1 in metastasis of HCC were investigated in vitro and in vivo. Our results showed that Ack1 was overexpressed in human HCC tissues and cell lines. High Ack1 expression was associated with HCC metastasis and determined as a significant and independent prognostic factor for HCC after liver resection. Ack1 promoted HCC invasion and metastasis in vitro and in vivo. Mechanistically, we confirmed that Ack1 enhanced invasion and metastasis of HCC via EMT by mediating AKT phosphorylation. In conclusion, our study shows Ack1 is a novel prognostic biomarker for HCC and promotes metastasis of HCC via EMT by activating AKT signaling.
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Lei
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Li
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Chen
authors.affiliation: Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
authors.forename: Di-Peng
authors.initials: DP
authors.lastname: Ou
authors.affiliation: Department of Abdominal Surgical Oncology, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, China.
authors.forename: Xiao-Xin
authors.initials: XX
authors.lastname: Qiu
authors.affiliation: Department of Abdominal Surgical Oncology, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, China.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Zuo
authors.affiliation: Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.|Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
authors.forename: Lian-Yue
authors.initials: LY
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D016022:Case-Control Studies; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D015331:Cohort Studies; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009367:Neoplasm Staging; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C403262:TNK2 protein, human
keywords: Ack1; hepatocellular carcinoma; metastasis; prognosis