title: Enzymatic Analysis of Positional Fatty Acid Distributions in Triacylglycerols by 1(3)-Selective Transesterification with Candida antarctica Lipase B: a Collaborative Study.
abstract: The positional distributions of fatty acids (FAs) in fats and oils are principally analyzed by selectively transesterifying the target triacylglycerols (TAGs) at the 1(3) position using Pseudozyma (Candida) antarctica lipase, followed by recovering the resulting 2-monoacylglycerols (MAGs) by chromatography. FA compositions were measured by gas chromatography (GC) after methylating target TAGs and 2-MAGs. The method was collaboratively evaluated by 12 laboratories by analyzing the positional FA distributions in soybean, palm, and sardine oils. The maximum reproducibility relative standard deviations for the major FAs and those at the sn-2 positions of soybean, palm, and sardine oils were 4.41% and 3.92% (18:3n-3), 4.48% and 3.82% (18:0), and 8.93 and 8.24% (14:0), respectively. The values at the sn-2 position were always low. Therefore, these results indicated that the variations were mainly caused by the FA analysis procedure, i.e., the methylation and GC analyses, rather than the enzymatic transesterification and chromatography utilized to prepare 2-MAGs from the target oil.
authors.affiliation: Osaka Municipal Technical Research Institute.
authors.forename: Yomi
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sato
authors.forename: Mihoko
authors.initials: M
authors.lastname: Asada
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Arishima
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Iida
authors.forename: Jun
authors.initials: J
authors.lastname: Imagi
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Saito
authors.forename: Takashi
authors.initials: T
authors.lastname: Sano
authors.forename: Akiko
authors.initials: A
authors.lastname: Sasaki
authors.forename: Ryo
authors.initials: R
authors.lastname: Sasaki
authors.forename: Chiemi
authors.initials: C
authors.lastname: Sato
authors.forename: Tadahisa
authors.initials: T
authors.lastname: Shibuya
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tsukahara
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Toru
authors.initials: T
authors.lastname: Fukazawa
authors.forename: Ryuji
authors.initials: R
authors.lastname: Hori
authors.forename: Rika
authors.initials: R
authors.lastname: Homma
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamashita
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Shimpei
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D002849:Chromatography, Gas; D057075:Enzyme Assays / Q000379:methods*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis*; D005395:Fish Oils / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D050178:Monoglycerides; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry*; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D005656:Fungal Proteins; D050178:Monoglycerides; D010938:Plant Oils; D014280:Triglycerides; D000073878:Palm Oil; D013024:Soybean Oil; D008049:Lipase; C431349:lipase B, Candida antarctica