title: Isolation and Functional Analysis of Human Neutrophils.
abstract: This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.
authors.affiliation: Neutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland.
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Kuhns
authors.affiliation: Neutrophil Monitoring Lab, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland.
authors.forename: Debra A Long
authors.initials: DAL
authors.lastname: Priel
authors.affiliation: Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Jessica
authors.initials: J
authors.lastname: Chu
authors.affiliation: Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Kol A
authors.initials: KA
authors.lastname: Zarember
mesh_terms: D002499:Centrifugation, Density Gradient / Q000379:methods*; D045304:Cytochromes c / Q000378:metabolism; D003911:Dextrans; D005362:Ficoll; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology; D011205:Povidone; D016897:Respiratory Burst / Q000502:physiology; D012822:Silicon Dioxide; D013211:Staphylococcus aureus / Q000276:immunology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D003911:Dextrans; D005362:Ficoll; C016039:Percoll; D012822:Silicon Dioxide; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D011205:Povidone
keywords: NOX2; innate immunity; leukocyte; neutrophil; phagocyte