title: The contribution of TRPC1, TRPC3, TRPC5 and TRPC6 to touch and hearing.
abstract: Transient receptor potential channels have diverse roles in mechanosensation. Evidence is accumulating that members of the canonical subfamily of TRP channels (TRPC) are involved in touch and hearing. Characteristic features of TRP channels include their high structural homology and their propensity to form heteromeric complexes which suggests potential functional redundancy. We previously showed that TRPC3 and TRPC6 double knockout animals have deficits in light touch and hearing whilst single knockouts were apparently normal. We have extended these studies to analyse deficits in global quadruple TRPC1, 3, 5 and 6 null mutant mice. We examined both touch and hearing in behavioural and electrophysiological assays, and provide evidence that the quadruple knockout mice have larger deficits than the TRPC3 TRPC6 double knockouts. Mechano-electrical transducer currents of cochlear outer hair cells were however normal. This suggests that TRPC1, TRPC3, TRPC5 and TRPC6 channels contribute to cutaneous and auditory mechanosensation in a combinatorial manner, but have no direct role in cochlear mechanotransduction.
authors.affiliation: Molecular Nociception Group, Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK. Electronic address: jane.sexton.09@ucl.ac.uk.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Sexton
authors.affiliation: Sussex Neuroscience, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
authors.forename: Terri
authors.initials: T
authors.lastname: Desmonds
authors.affiliation: Molecular Nociception Group, Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Quick
authors.affiliation: UCL Ear Institute, 332 Gray's Inn Road, London WC1X 8EE, UK.
authors.forename: Ruth
authors.initials: R
authors.lastname: Taylor
authors.affiliation: Neurobiology Laboratory, National Institute of Environmental Health Sciences, 111 TW Alexander Dr., Research Triangle Park, N.C. 27709, USA.
authors.forename: Joel
authors.initials: J
authors.lastname: Abramowitz
authors.affiliation: UCL Ear Institute, 332 Gray's Inn Road, London WC1X 8EE, UK.
authors.forename: Andy
authors.initials: A
authors.lastname: Forge
authors.affiliation: Sussex Neuroscience, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK; Dept. Otorhinolaryngology, University Medical Center Groningen, University of Groningen, 9700 RB Groningen, The Netherlands.
authors.forename: Corné J
authors.initials: CJ
authors.lastname: Kros
authors.affiliation: Neurobiology Laboratory, National Institute of Environmental Health Sciences, 111 TW Alexander Dr., Research Triangle Park, N.C. 27709, USA.
authors.forename: Lutz
authors.initials: L
authors.lastname: Birnbaumer
authors.affiliation: Molecular Nociception Group, Wolfson Institute for Biomedical Research, University College London, London WC1E 6BT, UK.
authors.forename: John N
authors.initials: JN
authors.lastname: Wood
mesh_terms: D000818:Animals; D016057:Evoked Potentials, Auditory, Brain Stem; D006198:Hair Cells, Auditory / Q000502:physiology; D006309:Hearing / Q000502:physiology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D050052:TRPC Cation Channels / Q000235:genetics / Q000502:physiology*; D000074287:TRPC6 Cation Channel; D014110:Touch / Q000502:physiology*; D014724:Vestibular Function Tests
chemical_list: D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D000074287:TRPC6 Cation Channel; C117032:TRPC6 protein, human; C112021:Trpc5 protein, mouse; C096351:transient receptor potential cation channel, subfamily C, member 1
keywords: Mechanosensation; TRP channels; Touch