title: Mucosal delivery switches the response to an adjuvanted tuberculosis vaccine from systemic TH1 to tissue-resident TH17 responses without impacting the protective efficacy.
abstract: Pulmonary tuberculosis (TB) remains one of the leading causes of infectious disease death despite widespread usage of the BCG vaccine. A number of new TB vaccines have moved into clinical evaluation to replace or boost the BCG vaccine including ID93+GLA-SE, an adjuvanted subunit vaccine. The vast majority of new TB vaccines in trials are delivered parenterally even though intranasal delivery can augment lung-resident immunity and protective efficacy in small animal models. Parenteral immunization with the adjuvanted subunit vaccine ID93+GLA-SE elicits robust TH1 immunity and protection against aerosolized Mycobacterium tuberculosis in mice and guinea pigs. Here we describe the immunogenicity and efficacy of this vaccine when delivered intranasally. Intranasal delivery switches the CD4 T cell response from a TH1 to a TH17 dominated tissue-resident response with increased frequencies of ID93-specific cells in both the lung tissue and at the lung surface. Surprisingly these changes do not affect the protective efficacy of ID93+GLA-SE. Unlike intramuscular immunization, ID93+GLA does not require the squalene-based oil-in-water emulsion SE to elicit protective CD4 T cells when delivered intranasally. Finally we demonstrate that TNF and the IL-17 receptor are dispensable for the efficacy of the intranasal vaccine suggesting an alternative mechanism of protection.
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA; Department of Global Health, University of Washington, Seattle, WA 98105, USA. Electronic address: Mark.orr@idri.org.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Orr
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: Elyse A
authors.initials: EA
authors.lastname: Beebe
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hudson
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: David
authors.initials: D
authors.lastname: Argilla
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: Po-Wei D
authors.initials: PW
authors.lastname: Huang
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA.
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Reese
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA; Department of Global Health, University of Washington, Seattle, WA 98105, USA.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Fox
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA; Department of Global Health, University of Washington, Seattle, WA 98105, USA.
authors.forename: Steven G
authors.initials: SG
authors.lastname: Reed
authors.affiliation: Infectious Disease Research Institute, Seattle, WA 98102, USA; Department of Global Health, University of Washington, Seattle, WA 98105, USA.
authors.forename: Rhea N
authors.initials: RN
authors.lastname: Coler
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D058956:Administration, Mucosal; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001500:BCG Vaccine / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016207:Cytokines; D010446:Peptide Fragments; D032581:Tuberculosis Vaccines; D014409:Tumor Necrosis Factor-alpha; C076419:tumor necrosis factor (1-26)
keywords: ID93+GLA-SEi; Intranasal; Mucosal; TH17; Tuberculosis