title: A Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System.
abstract: BACKGROUND
  Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells.
  
  
  METHODS
  The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for transfection of Spodoptera frugiperda cells.
  
  
  RESULTS
  Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
  
  
  CONCLUSIONS
  The application of copGFP control bacmid can be considered as an appropriate control for insect cell transfection.
authors.affiliation: Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Narjes
authors.initials: N
authors.lastname: Shokrollahi
authors.affiliation: Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Delavar
authors.initials: D
authors.lastname: Shahbazzadeh
authors.affiliation: Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Kamran
authors.initials: K
authors.lastname: Pooshang-Bagheri
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Habibi-Anbouhi
authors.affiliation: Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jahanian-Najafabadi
authors.affiliation: Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Behdani
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D033342:Copepoda / Q000235:genetics; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000096:biosynthesis* / Q000235:genetics*; D061987:Sf9 Cells; D018411:Spodoptera / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins
keywords: Baculovirus; Sf9; Transfection