title: Simple HPLC-UV for the quantification of a new leishmanicidal candidate (E)-1-4(trifluoromethyl) benzylidene)-5-(2-4-dichlorozoyl) carbonylhydrazine (LASSBio-1736) in rat plasma for pharmacokinetics assessment.
abstract: In this study, a sensitive HPLC-UV assay was developed and validated for the determination of LASSBio-1736 in rat plasma with sodium diclofenac as internal standard (IS). Liquid-liquid extraction using acetonitrile was employed to extract LASSBio-1736 and IS from 100 μL of plasma previously basified with NaOH 0.1 M. Chromatographic separation was carried on Waters Spherisorb(®) S5 ODS2 C18 column (150 × 4.6 mm, 5 μm) using an isocratic mobile phase composed by water with triethylamine 0.3% (pH 4), methanol and acetonitrile grade (45:15:40, v/v/v) at a flow rate of 1 mL/min. Both LASSBio-1736 and IS were eluted at 4.2 and 5 min, respectively, with a total run time of 8 min only. The lower limit of quantification was 0.2 μg/mL and linearity between 0.2 and 4 μg/mL was obtained, with an R(2)  > 0.99. The accuracy of the method was >90.5%. The relative standard deviations intra and interday were <6.19 and <7.83%, respectively. The method showed the sensitivity, linearity, precision, accuracy and selectivity required to quantify LASSBio-1736 in preclinical pharmacokinetic studies according to the criteria established by the US Food and Drug Administration and European Medicines Agency. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Curso de Farmácia, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Barbra Katyuschya Sanches
authors.initials: BKS
authors.lastname: Moraes
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Lisiane
authors.initials: L
authors.lastname: Bajerski
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Alcides
authors.initials: A
authors.lastname: Parisotto
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Carlos Eduardo da Rosa
authors.initials: CEDR
authors.lastname: Silva
authors.affiliation: Laboratório de Avaliação e Síntese de Substâncias Bioativas - Universidade Federal do Rio de Janeiro, UFRJ, Brasil.
authors.forename: Marina Amaral
authors.initials: MA
authors.lastname: Alves
authors.affiliation: Laboratório de Avaliação e Síntese de Substâncias Bioativas - Universidade Federal do Rio de Janeiro, UFRJ, Brasil.
authors.forename: Eliezer de Jesus
authors.initials: EJ
authors.lastname: Barreiro
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Rodrigo José
authors.initials: RJ
authors.lastname: Freddo
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, UFRGS, Av.Ipiranga, 2752, 1. andar, CEP, 90610-000, Porto Alegre, Brasil.
authors.forename: Teresa
authors.initials: T
authors.lastname: Dalla Costa
authors.affiliation: Laboratório de Avaliação e Síntese de Substâncias Bioativas - Universidade Federal do Rio de Janeiro, UFRJ, Brasil.
authors.forename: Lídia Moreira
authors.initials: LM
authors.lastname: Lima
authors.affiliation: Curso de Farmácia, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.|Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Br 472, Km 592, Uruguaiana, CEP, 97500-970, Brasil.
authors.forename: Sandra Elisa
authors.initials: SE
authors.lastname: Haas
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006834:Hydrazines / Q000493:pharmacokinetics / Q000494:pharmacology*; D007891:Leishmania / Q000187:drug effects*; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D006834:Hydrazines
keywords: HPLC; LASSBio-1736; Leishmaniosis; pharmacokinetics