title: Continuous harvest of stem cells via partial detachment from thermoresponsive nanobrush surfaces.
abstract: Stem cell culture is typically based on batch-type culture, which is laborious and expensive. Here, we propose a continuous harvest method for stem cells cultured on thermoresponsive nanobrush surfaces. In this method, stem cells are partially detached from the nanobrush surface by reducing the temperature of the culture medium below the critical solution temperature needed for thermoresponse. The detached stem cells are harvested by exchange into fresh culture medium. Following this, the remaining cells are continuously cultured by expansion in fresh culture medium at 37 °C. Thermoresponsive nanobrush surfaces were prepared by coating block copolymers containing polystyrene (for hydrophobic anchoring onto culture dishes) with three types of polymers: (a) polyacrylic acid with cell-binding oligopeptides, (b) thermoresponsive poly-N-isopropylacrylamide, and (c) hydrophilic poly(ethyleneglycol)methacrylate. The optimal coating durations and compositions for these copolymers to facilitate adequate attachment and detachment of human adipose-derived stem cells (hADSCs) and embryonic stem cells (hESCs) were determined. hADSCs and hESCs were continuously harvested for 5 and 3 cycles, respectively, via the partial detachment of cells from thermoresponsive nanobrush surfaces.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: I-Chia
authors.initials: IC
authors.lastname: Peng
authors.affiliation: Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, 200, Chung-Bei Rd., Chungli, Taoyuan 320, Taiwan, ROC.
authors.forename: Chin-Chen
authors.initials: CC
authors.lastname: Yeh
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: Yi-Tung
authors.initials: YT
authors.lastname: Lu
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: Saradaprasan
authors.initials: S
authors.lastname: Muduli
authors.affiliation: Cathay Medical Research Institute, Cathay General Hospital, No. 32, Ln 160, Jian-Cheng Road, Hsi-Chi City, Taipei 221, Taiwan, ROC; Institute of Systems Biology and Bioinformatics, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Alarfaj
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Murugan A
authors.initials: MA
authors.lastname: Munusamy
authors.affiliation: Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
authors.forename: S Suresh
authors.initials: SS
authors.lastname: Kumar
authors.affiliation: Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu 641 046, India.
authors.forename: Kadarkarai
authors.initials: K
authors.lastname: Murugan
authors.affiliation: Department of Surgery, Cathay General Hospital, No.280, Sec. 4, Ren'ai Rd., Da'an Dist., Taipei 10693, Taiwan, ROC; Graduate Institute of Translational and Interdisciplinary Medicine, College of Health Science and Technology, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC.
authors.forename: Hsin-chung
authors.initials: HC
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, 200, Chung-Bei Rd., Chungli, Taoyuan 320, Taiwan, ROC. Electronic address: changyung0307@gmail.com.
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan, ROC; Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia; Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-098, Japan. Electronic address: higuchi@ncu.edu.tw.
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002448:Cell Adhesion; D003470:Culture Media; D006801:Humans; D036103:Nanotechnology; D013234:Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media
keywords: Biomaterial; Cell culture; Embryonic stem cell; Nanobrush; Stem cell; Thermoresponsive