title: In Vitro Therapeutic Potential of Tio2 Nanoparticles Against Human Cervical Carcinoma Cells.
abstract: Cellular and physiological responses to the degradation products of titanium implants are key indicators to determine the quality of biocompatibility of implant devices. The present study investigated titanium dioxide (TiO2) nanoparticle-induced cytotoxicity, apoptotic morphological modification, and apoptotic-related gene expressions in the human cervical carcinoma cells. TiO2 nanoparticle-induced cytotoxicity on cancer cells was determined by the sulphorhodamine-B assay. Apoptotic morphological modification such as nuclear fragmentation, rounding, cytoplasm shrinkage, loss of adhesion, and reduced cell volume were observed by an inverted, fluorescence, and confocal laser scanning microscope (CLSM). The DNA fragmentation study showed the occurrence of necrosis and apoptosis in nanoparticle-treated cells. The qPCR study showed the increased p53 and bax mRNA expression in the nanoparticle-treated cells compared to control. In addition, caspase 3 activity was increased in nanoparticle-treated cells, which indicates the increased auto-catalysis. Taking all these data together, it may suggest that TiO2 nanoparticle could inhibit the growth of HeLa cells.
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Muthuraman
authors.initials: M
authors.lastname: Pandurangan
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Gansukh
authors.initials: G
authors.lastname: Enkhtaivan
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Jung A
authors.initials: JA
authors.lastname: Young
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Hur Ji
authors.initials: HJ
authors.lastname: Hoon
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: Hannah
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea.
authors.forename: SooBin
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, South Korea. frenzram1980@gmail.com.
authors.forename: Doo Hwan
authors.initials: DH
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D013329:Structure-Activity Relationship; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000473:pathology*
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: Apoptosis; Caspase 3; HeLa cells; SRB assay; TiO2 nanoparticles; mRNA