title: Linker histones in hormonal gene regulation.
abstract: In the present review, we summarize advances in our knowledge on the role of the histone H1 family of proteins in breast cancer cells, focusing on their response to progestins. Histone H1 plays a dual role in gene regulation by hormones, both as a structural component of chromatin and as a dynamic modulator of transcription. It contributes to hormonal regulation of the MMTV promoter by stabilizing a homogeneous nucleosome positioning, which reduces basal transcription whereas at the same time promoting progesterone receptor binding and nucleosome remodeling. These combined effects enhance hormone dependent gene transcription, which eventually requires H1 phosphorylation and displacement. Various isoforms of histone H1 have specific functions in differentiated breast cancer cells and compact nucleosomal arrays to different extents in vitro. Genome-wide studies show that histone H1 has a key role in chromatin dynamics of hormone regulated genes. A complex sequence of enzymatic events, including phosphorylation by CDK2, PARylation by PARP1 and the ATP-dependent activity of NURF, are required for H1 displacement and gene de-repression, as a prerequisite for further nucleosome remodeling. Similarly, during hormone-dependent gene repression a dedicated enzymatic mechanism controls H1 deposition at promoters by a complex containing HP1γ, LSD1 and BRG1, the ATPase of the BAF complex. Thus, a broader vision of the histone code should include histone H1, as the linker histone variants actively participate in the regulation of the chromatin structure. How modifications of the core histones tails affect H1 modifications and vice versa is one of the many questions that remains to be addressed to provide a more comprehensive view of the histone cross-talk mechanisms.
authors.affiliation: Centre de Regulació Genòmica (CRG), Spain; Universitat Pompeu Fabra (UPF), Dr. Aiguader 88, E-08003, Barcelona, Spain.
authors.forename: G P
authors.initials: GP
authors.lastname: Vicent
authors.affiliation: Centre de Regulació Genòmica (CRG), Spain; Universitat Pompeu Fabra (UPF), Dr. Aiguader 88, E-08003, Barcelona, Spain.
authors.forename: R H G
authors.initials: RH
authors.lastname: Wright
authors.affiliation: Centre de Regulació Genòmica (CRG), Spain; Universitat Pompeu Fabra (UPF), Dr. Aiguader 88, E-08003, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Beato
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry; D005786:Gene Expression Regulation; D006657:Histones / Q000502:physiology*; D006801:Humans; D009707:Nucleosomes / Q000502:physiology; D010766:Phosphorylation
chemical_list: D002843:Chromatin; D006657:Histones; D009707:Nucleosomes
keywords: Breast cancer cells; Chromatin remodeling; Gene regulation; Histone H1 variants; Linker histones; Nucleosomes; Progesterone receptor (PR)