title: Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells.
abstract: Recent studies show that long non-coding RNAs (lncRNAs) may be significant functional regulators in tumor development, including bladder cancer. Here, we found that PVT1 was upregulated in bladder cancer tissues and cells. Further experiments revealed that PVT1 promoted cell proliferation and suppressed cell apoptosis. Furthermore we also used the emerging technology, synthetic biology, to create tetracycline-inducible small hairpin RNA (shRNA) vectors which silenced PVT1 in a dosage-dependent manner to inhibit the progression of bladder cancer. In conclusion, data suggest that PVT1 could be an oncogene and may be a therapeutic target in bladder cancer. Synthetic "tetracycline-on" switch system can be used to quantitatively control the expression of PVT1 in bladder cancer in response to different concentration of doxycycline to suppress the progression of bladder cancer.
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.|Shantou University Medical College, Shantou 515041, Guangdong Province, People's Republic of China.
authors.forename: Chengle
authors.initials: C
authors.lastname: Zhuang
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.|Shantou University Medical College, Shantou 515041, Guangdong Province, People's Republic of China.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.|Anhui Medical University, Hefei 230601, Anhui Province, People's Republic of China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Xing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Junhao
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Centerat Shanghai, Shanghai 200000, Shanghai, People's Republic of China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, Shenzhen 518039, Guangdong Province, People's Republic of China.
authors.forename: Weiren
authors.initials: W
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D062085:RNA, Long Noncoding / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013752:Tetracycline / Q000494:pharmacology; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C521123:PVT1 long-non-coding RNA, human; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D013752:Tetracycline
keywords: PVT1; bladder cancer; lncRNAs; synthetic biology; tetracycline-inducible