title: T-cell activation or tolerization: the Yin and Yang of bacterial superantigens.
abstract: Bacterial superantigens (SAg) are exotoxins from pathogens which interact with innate and adaptive immune cells. The paradox that SAgs cause activation and inactivation/anergy of T-cells was soon recognized. The structural and molecular events following SAg binding to antigen presenting cells (APCs) followed by crosslinking of T-cell receptors were characterized in detail. Activation, cytokine burst and T-cell anergy have been described in vitro and in vivo. Later it became clear that SAg-induced T-cell anergy is in part caused by SAg-dependent activation of T-regulatory cells (Tregs). Although the main focus of analyses was laid on T-cells, it was also shown that SAg binding to MHC class II molecules on APCs induces a signal, which leads to activation and secretion of pro-inflammatory cytokines. Accordingly APCs are mandatory for T-cell activation. So far it is not known, whether APCs play a role during SAg-triggered activation of Tregs. We therefore tested whether in SAg (Streptococcal pyrogenic exotoxin A) -treated APCs an anti-inflammatory program is triggered in addition. We show here that not only the anti-inflammatory cytokine IL-10 and the co-inhibitory surface molecule PD-L1 (CD274) but also inhibitory effector systems like indoleamine 2,3-dioxygenase (IDO) or intracellular negative feedback loops (suppressor of cytokine signaling molecules, SOCS) are induced by SAgs. Moreover, cyclosporine A completely prevented induction of this program. We therefore propose that APCs triggered by SAgs play a key role in T-cell activation as well as inactivation and induction of Treg cells.
authors.affiliation: Medical Microbiology and Hygiene, Department of Infectious Diseases, University Hospital Heidelberg Heidelberg, Germany.
authors.forename: Aline
authors.initials: A
authors.lastname: Sähr
authors.affiliation: Medical Microbiology and Hygiene, Department of Infectious Diseases, University Hospital Heidelberg Heidelberg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Förmer
authors.affiliation: Medical Microbiology and Hygiene, Department of Infectious Diseases, University Hospital Heidelberg Heidelberg, Germany.
authors.forename: Dagmar
authors.initials: D
authors.lastname: Hildebrand
authors.affiliation: Medical Microbiology and Hygiene, Department of Infectious Diseases, University Hospital Heidelberg Heidelberg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Heeg
keywords: IDO; PD-L1; STAT3; Treg; anergy; class II signaling; co-inhibitory molecules; superantigen