title: [Genetic Characterization of Hemagglutinin on Measles Virus Epidemic Strain Genotype H1a in Liaoning Province (China) from 1997 to 2014].
abstract: To wished to characterize the hemagglutinin (H) gene of the measles virus epidemic strain H1a in Liaoning Province (China) from 1997-2014 to provide a basis for the control and elimination of measles. All 63 measles virus strains were the H1a genotype. Fragments of the H gene (1854 nucleotides) and nucleoprotein (N) gene (450 nucleotides) were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the PCR products sequenced and analyzed. Phylogenetic-trees were constructed with reference strains of the genotype-H measles virus downloaded from GenBank, including Chinese measles virus strains isolated in 1993-1994 and the vaccine reference strains S-191 and C-47. Sixty-three strains of the measles virus in 1997-2014 belonged to genotype H1a. The mean evolutionary rate for gene N-450 was higher than that for the H gene. All 63 strains of the measles virus were mutated from: serine (Ser S) to asparagine (Asn N) in the 240th amino acid; arginine (Arg R) to glycine (Gly G) in the 243th; and tyrosine (Tyr Y) to Asn N in the 481th amino acid. All measles virus strains in cluster 2 were mutated from proline (Pro P) to leucine (Leu L) in the 397th amino acid. The other neutralization sites showed no apparent difference when comparing the nucleotides/amino acids of the H gene of S191 vaccine strains.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Hao
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.forename: Wenqing
authors.initials: W
authors.lastname: Yao
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D002681:China / Q000453:epidemiology; D030541:Databases, Genetic; D058872:Epidemics; D019143:Evolution, Molecular; D005838:Genotype; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000235:genetics*; D008457:Measles / Q000453:epidemiology*; D008459:Measles virus / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny
chemical_list: D006389:Hemagglutinins, Viral