title: Prevalence Analysis and Molecular Characterization of Salmonella at Different Processing Steps in Broiler Slaughter Plants in South Korea.
abstract: In this study, changes in the prevalence of Salmonella during the processing of broiler chicken carcasses were investigated. A total of 1040 fecal swabs and chicken carcasses samples were collected from 2 processing plants at the 4 stages of broiler processing, which included live birds in slaughter line, postevisceration/prewashing, postwashing/prechilling, and postchilling, respectively. The intraspecific biodiversity of the Salmonella isolates was determined using a DiversiLab automated repetitive sequence-based PCR (rep-PCR) system. In both plants, the prevalence of Salmonella increased considerably after evisceration (from 4.6% to 30.8%, P < 0.05) and decreased after washing (from 30.8% to 25.4%, P < 0.05). However, the chilling step had little effect on Salmonella prevalence (from 25.4% to 22.7%, P > 0.05). The most frequent Salmonella serovar in plant A was Infantis (35.8%), followed by Enteritidis (26.2%) and Montevideo (15.0%), while Montevideo (43.6%) and Enteritidis (35.9%) were most prevalent in plant B. A difference in the rep-PCR banding pattern was found to be related to the processing plant origin and serovar rather than sampling point or sampling day, although there were some exceptional strains.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: KU Center for Food Safety, College of Veterinary Medicine, Konkuk Univ, Seoul, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lim
authors.affiliation: KU Center for Food Safety, College of Veterinary Medicine, Konkuk Univ, Seoul, Republic of Korea.
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
authors.affiliation: Ministry of Food and Drug safety, Cheongju, Republic of Korea.
authors.forename: Young-Jo
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Ministry of Food and Drug safety, Cheongju, Republic of Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Heo
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Wee
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, U.S.A.
authors.forename: Kidon
authors.initials: K
authors.lastname: Sung
authors.affiliation: Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Jin-San
authors.initials: JS
authors.lastname: Moon
mesh_terms: D000003:Abattoirs; D000818:Animals; D002645:Chickens; D003080:Cold Temperature; D004203:Disinfection; D005511:Food Handling; D005516:Food Microbiology; D006801:Humans; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D056910:Republic of Korea; D012475:Salmonella / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D065288:Serogroup
keywords: contamination; poultry processing; salmonella; subtyping