title: Effect of Lipopolysaccharide on the Progression of Non-Alcoholic Fatty Liver Disease in High Caloric Diet-Fed Mice.
abstract: The incidence of non-alcoholic steatohepatitis (NASH) is increasing. Because gut microbiota have been highlighted as one of the key factors in the pathogenesis of metabolic syndrome, we investigated the involvement of the bacterial component in the progression of non-alcoholic fatty liver (NAFL) to NASH. C57BL/6 mice were fed with maintenance food (MF, groups A and B) or a high caloric diet (HCD, groups C and D) for 1 month. Mice were then divided into four groups: Groups A and C were inoculated with PBS, while groups B and D were inoculated with lipopolysaccharide (LPS) plus complete Freund's adjuvant (CFA). The inoculations were performed a total of 3 times over 3 months. At 6 months, while hepatic steatosis was observed in groups C and D, cellular infiltration and fibrosis were less evident in group C than in group D. Inflammatory cytokines were upregulated in groups B and D. 16S rRNA pyrosequencing of whole colon homogenates containing faeces showed that certain bacterial groups, such as Bacteroidaceae, Peptostreptococcaceae and Erysipelotrichaceae, were increased in groups C and D. Although loading of bacterial components (LPS) resulted in hepatic inflammation in both MF- and HCD-fed mice, HCD feeding was more crucial in the progression of NAFL during the triggering phase.
authors.affiliation: Support Center for Women Health Care Professionals and Researchers, Tokyo Women's Medical University, Tokyo, Japan.|Department of Medicine and Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsushita
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.|Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Osaka
authors.affiliation: Department of Medicine and Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.|Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Haruta
authors.affiliation: Institute of Laboratory Animals, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueshiba
authors.affiliation: Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yanagisawa
authors.affiliation: Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Omori-Miyake
authors.affiliation: Department of Medicine and Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Hashimoto
authors.affiliation: Department of Pathology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.affiliation: Department of Medicine and Gastroenterology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tokushige
authors.affiliation: Support Center for Women Health Care Professionals and Researchers, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsuneda
authors.affiliation: Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Yagi
mesh_terms: D000818:Animals; D003106:Colon / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000235:genetics; D004032:Diet / Q000009:adverse effects; D004195:Disease Models, Animal; D018450:Disease Progression; D002149:Energy Intake; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S