title: Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.
abstract: Cells have evolved oscillators with different frequencies to coordinate periodic processes. Here we studied the interaction of two oscillators, the cell division cycle (CDC) and the yeast metabolic cycle (YMC), in budding yeast. Previous work suggested that the CDC and YMC interact to separate high oxygen consumption (HOC) from DNA replication to prevent genetic damage. To test this hypothesis, we grew diverse strains in chemostat and measured DNA replication and oxygen consumption with high temporal resolution at different growth rates. Our data showed that HOC is not strictly separated from DNA replication; rather, cell cycle Start is coupled with the initiation of HOC and catabolism of storage carbohydrates. The logic of this YMC-CDC coupling may be to ensure that DNA replication and cell division occur only when sufficient cellular energy reserves have accumulated. Our results also uncovered a quantitative relationship between CDC period and YMC period across different strains. More generally, our approach shows how studies in genetically diverse strains efficiently identify robust phenotypes and steer the experimentalist away from strain-specific idiosyncrasies.
authors.affiliation: Program in Cellular & Molecular Biology, Duke University, Durham, NC 27708 University Program in Genetics & Genomics, Duke University, Durham, NC 27708 Center for Genomic & Computational Biology, Duke University, Durham, NC 22710 Department of Biology, Duke University, Durham, NC 27708.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Burnetti
authors.affiliation: Center for Genomic & Computational Biology, Duke University, Durham, NC 22710 Department of Biology, Duke University, Durham, NC 27708.
authors.forename: Mert
authors.initials: M
authors.lastname: Aydin
authors.affiliation: Center for Genomic & Computational Biology, Duke University, Durham, NC 22710 Department of Biology, Duke University, Durham, NC 27708 nicolas.buchler@duke.edu.
authors.forename: Nicolas E
authors.initials: NE
authors.lastname: Buchler
mesh_terms: D001683:Biological Clocks / Q000502:physiology; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D004261:DNA Replication; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D010313:Partial Pressure; D004718:Saccharomycetales / Q000166:cytology* / Q000378:metabolism*
chemical_list: D010100:Oxygen