title: Green Tea Extract Rich in Epigallocatechin-3-Gallate Prevents Fatty Liver by AMPK Activation via LKB1 in Mice Fed a High-Fat Diet.
abstract: Supplementation with epigallocatechin-3-gallate has been determined to aid in the prevention of obesity. Decaffeinated green tea extract appears to restore a normal hepatic metabolic profile and attenuate high-fat diet (HFD)-induced effects, thereby preventing non-alcoholic fatty liver disease in mice. Mice were maintained on either a control diet (CD) or HFD for 16 weeks and supplemented with either water or green tea extract (50 mg/kg/day). The body mass increase, serum adiponectin level, and lipid profile were measured over the course of the treatment. Furthermore, the AMPK pathway protein expression in the liver was measured. From the fourth week, the weight gain in the CD + green tea extract (CE) group was lower than that in the CD + water (CW) group. From the eighth week, the weight gain in the HFD + water (HFW) group was found to be higher than that in the CW group. Moreover, the weight gain in the HFD + green tea extract (HFE) group was found to be lower than that in the HFW group. Carcass lipid content was found to be higher in the HFW group than that in the CW and HFE groups. Serum analysis showed reduced non-esterified fatty acid level in the CE and HFE groups as compared with their corresponding placebo groups. Increased adiponectin level was observed in the same groups. Increased VLDL-TG secretion was observed in the HFW group as compared with the CW and HFE groups. Increased protein expression of AdipoR2, SIRT1, pLKB1, and pAMPK was observed in the HFE group, which explained the reduced expression of ACC, FAS, SREBP-1, and ChREBP in this group. These results indicate that the effects of decaffeinated green tea extract may be related to the activation of AMPK via LKB1 in the liver of HFD-fed mice.
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Aline B
authors.initials: AB
authors.lastname: Santamarina
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Juliana L
authors.initials: JL
authors.lastname: Oliveira
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Fernanda P
authors.initials: FP
authors.lastname: Silva
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: June
authors.initials: J
authors.lastname: Carnier
authors.affiliation: Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde-Universidade Federal de São Paulo, Santos, Brazil.
authors.forename: Laís V
authors.initials: LV
authors.lastname: Mennitti
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Aline A
authors.initials: AA
authors.lastname: Santana
authors.affiliation: Laboratório de Movimento Humano-Universidade São Judas Tadeu, São Paulo, Brazil.
authors.forename: Gabriel H I
authors.initials: GH
authors.lastname: de Souza
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Eliane B
authors.initials: EB
authors.lastname: Ribeiro
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Cláudia M
authors.initials: CM
authors.lastname: Oller do Nascimento
authors.affiliation: Exercise and Immunometabolism Research Group, Department of Physical Education, Universidade Estadual Paulista, UNESP, Presidente Prudente, SP, Brazil.
authors.forename: Fábio S
authors.initials: FS
authors.lastname: Lira
authors.affiliation: Departamento de Fisiologia-Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Lila M
authors.initials: LM
authors.lastname: Oyama
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D059305:Diet, High-Fat / Q000009:adverse effects*; D004789:Enzyme Activation; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013662:Tea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate; D017346:Protein Serine-Threonine Kinases; C491654:Stk11 protein, mouse; D055372:AMP-Activated Protein Kinases