title: LC-MS-MS Method for Stimulants in Wastewater During Football Games.
abstract: A method was developed for the analysis of amphetamines and cocaine (Coc) in wastewater samples using liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS). Seven stimulant-type drugs and metabolites were analyzed. These drugs included amphetamine (Amp), methamphetamine (Meth), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), methylenedioxyethylamphetamine (MDEA), Coc and benzoylecgonine (BE, the major metabolite of Coc). These drugs were chosen because of their widespread use. Wastewater samples were collected at both the Oxford Waste Water Treatment Plant in Oxford, Mississippi (MS) and the University Wastewater Treatment Plant in University, MS. Samples were collected on weekends in which the Ole Miss Rebel football team held home games (Vaught-Hemingway Stadium, University, MS 38677). The collected samples were analyzed using a validated method and found to contain Amp, Meth, MDMA, Coc and BE. The concentrations of Amp and BE significantly rose in the university wastewater during football games.
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.
authors.forename: Waseem
authors.initials: W
authors.lastname: Gul
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA.
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Stamper
authors.affiliation: Department of Chemistry and Biochemistry, University of Mississippi, University, MS 38677, USA.
authors.forename: Murrell
authors.initials: M
authors.lastname: Godfrey
authors.affiliation: ElSohly Laboratories, Inc., 5 Industrial Park Drive, Oxford, MS 38655, USA National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, MS 38677, USA elsohly@elsohly.com.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: ElSohly
mesh_terms: D000662:Amphetamines / Q000032:analysis*; D000697:Central Nervous System Stimulants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis; D005538:Football; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013287:Illicit Drugs; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014495:Universities; D062065:Wastewater / Q000032:analysis*
chemical_list: D000662:Amphetamines; D000697:Central Nervous System Stimulants; D003042:Cocaine; D013287:Illicit Drugs; D062065:Wastewater; C005618:benzoylecgonine