title: Trypanosoma cruzi mitochondrial tryparedoxin peroxidase is located throughout the cell and its pull down provides one step towards the understanding of its mechanism of action.
abstract: Trypanosoma cruzi depends on the effectiveness of redox metabolism to survive and ensure infection in the host. Homeostasis of redox metabolism in T. cruzi is achieved by the actions of several proteins that differ in many aspects from host proteins. Although extensive research has been performed examining hydroperoxide cytosolic antioxidant defense centered on trypanothione, the mechanisms of mitochondrial antioxidant defense are not yet known. The aim of this study was to elucidate the partners of TcMPx antioxidant pathway and to determine the influence of the cellular context (physiological versus oxidative stress). Through co-precipitation coupled with a mass spectrometry approach, a variety of proteins were detected under physiological and oxidative stress conditions. Interestingly, functional category analysis of the proteins identified under physiological conditions showed that they were involved in the stress response, oxidoreduction, thiol transfer, and metabolic processes; this profile is distinct under oxidative stress conditions likely due to structural alterations. Our findings help to elucidate the reactions involving TcMPx and most importantly also reveal that this protein is present throughout the cell and that its interaction partners change following oxidative stress exposure. The involvement and significance of the proteins found to interact with TcMPx and other possible functions for this protein are discussed widening our knowledge regarding T. cruzi mitochondrial antioxidant defenses.
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Peloso
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Dias
authors.affiliation: Laboratório de Bioquímica e Química de Proteínas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: R M L
authors.initials: RM
authors.lastname: Queiroz
authors.affiliation: Centro Nacional de Pesquisa em Energia e Materiais, Laboratório Nacional de Biociências, Campinas, SP, Brazil.
authors.forename: A F P Paes
authors.initials: AF
authors.lastname: Leme
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil.
authors.forename: C N
authors.initials: CN
authors.lastname: Pereira
authors.affiliation: Centro Nacional de Pesquisa em Energia e Materiais, Laboratório Nacional de Biociências, Campinas, SP, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Carnielli
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Werneck
authors.affiliation: Laboratório de Bioquímica e Química de Proteínas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: M V
authors.initials: MV
authors.lastname: Sousa
authors.affiliation: Laboratório de Bioquímica e Química de Proteínas, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: C A O
authors.initials: CA
authors.lastname: Ricart
authors.affiliation: Departamento de Bioquímica e Biologia Tecidual, UNICAMP, Campinas, SP, Brazil. Electronic address: frgad@unicamp.br.
authors.forename: F R
authors.initials: FR
authors.lastname: Gadelha
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000201:enzymology*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D014349:Trypanosoma cruzi / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D016877:Oxidants; D020543:Proteome; D015800:Protozoan Proteins; D006861:Hydrogen Peroxide; D010544:Peroxidases; C110959:tryparedoxin peroxidase
keywords: Interactome; Mitochondrial tryparedoxin peroxidase; Trypanosoma cruzi