title: Electroacupuncture Upregulates SIRT1-Dependent PGC-1α Expression in SAMP8 Mice.
abstract: BACKGROUND
  Abnormalities of brain energy metabolism are involved in Alzheimer disease (AD). Sirtuin 1 (SIRT1) is a class III histone deacetylase and activates peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α), which enhances mitochondrial biogenesis and energy homeostasis. Electroacupuncture (EA) has been reported to improve brain energy metabolism in AD. However, the effect of EA on SIRT1 and PGC-1α in AD remains unclear.
  
  
  METHODS
  ATP levels were measured using assay kits in the hippocampus and frontal cortex of senescence-accelerated mouse prone 8 (SAMP8) mice. Western blotting analysis and quantitative real-time RT-PCR were performed to measure the expression of SIRT1 and PGC-1a in the hippocampus of SAMP8 mice. PGC-1α acetylation was analyzed using immunoprecipitation.
  
  
  RESULTS
  Compared with senescence-accelerated resistant mice 1 (SAMR1) mice, SAMP8 mice had a decline in ATP levels and the expression of SIRT1 and PGC-1α. EA treatment improved ATP levels, upregulated the expression of SIRT1 and PGC-1α, and decreased PGC-1α acetylation.
  
  
  CONCLUSIONS
  These data suggest that EA improved brain energy metabolism, potentially associated with the upregulation of SIRT1-dependent PGC-1α expression.
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Weiguo
authors.initials: W
authors.lastname: Dong
authors.affiliation: The Third People's Hospital of Fujian Province, Fuzhou, Fujian, China (mainland).
authors.forename: Wanqing
authors.initials: W
authors.lastname: Quo
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Changzheng
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Yongcai
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Xuehua
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China (mainland).
authors.forename: Hong
authors.initials: H
authors.lastname: Shi
mesh_terms: D000107:Acetylation; D000255:Adenosine Triphosphate / Q000737:chemistry; D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D015671:Electroacupuncture; D005625:Frontal Lobe / Q000378:metabolism; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism; D006706:Homeostasis; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D056564:Sirtuin 1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C485290:Ppargc1a protein, mouse; D014157:Transcription Factors; D000255:Adenosine Triphosphate; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1