title: Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects.
abstract: OBJECTIVE
  To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency.
  
  
  METHODS
  Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (V max), and an affinity constant (K m ) were assessed with a [(3)H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated.
  
  
  RESULTS
  Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [(3)H] 5-HT uptake was observed (Spearman's correlation R (2) = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, V max, and K m values (p = 0.08, 0.12, 0.71, and 0.68, respectively).
  
  
  CONCLUSIONS
  Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. hajime@md.okayama-u.ac.jp.|Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan. hajime@md.okayama-u.ac.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Minakuchi
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Sogawa
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Miki
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Emilio S
authors.initials: ES
authors.lastname: Hara
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Maekawa
authors.affiliation: Department of Dental Pharmacology, Matsumoto Dental University, 1780 Hiro-okagohbara, Shiojiri city, Nagano, 399-0781, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Sogawa
authors.affiliation: Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kitayama
authors.affiliation: Department of Stomatgnathic Function and Occlusal Reconstruction, Institute of Biomedical Science, Clinical Dentistry, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima City, 770-8504, Japan.
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Matsuka
authors.affiliation: Advanced Program in Orofacial Pain and Oral Medicine, Ostrow School of Dentistry, University of Southern California, 925 West 34th St., Los Angeles, CA, 90089, USA.
authors.forename: Glenn Thomas
authors.initials: GT
authors.lastname: Clark
authors.affiliation: Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8525, Japan.
authors.forename: Takuo
authors.initials: T
authors.lastname: Kuboki
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D004576:Electromyography; D005260:Female; D006801:Humans; D008297:Male; D010976:Platelet Count; D017286:Polysomnography; D012701:Serotonin / Q000097:blood; D050486:Serotonin Plasma Membrane Transport Proteins / Q000097:blood*; D020186:Sleep Bruxism / Q000097:blood* / Q000453:epidemiology*; D013223:Statistics as Topic; D055815:Young Adult
chemical_list: D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin
keywords: Electromyography (EMG); Human serotonin transporter; Peripheral platelet; Sleep bruxism; Uptake ability