title: Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato.
abstract: Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding β-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.
authors.affiliation: Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea.
authors.forename: A Min
authors.initials: AM
authors.lastname: Kwak
authors.affiliation: Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea.
authors.forename: Kyeong Jin
authors.initials: KJ
authors.lastname: Min
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA), Wanju 55365, Korea.
authors.forename: Sang Yeop
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea. ; Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea.
authors.forename: Hee Wan
authors.initials: HW
authors.lastname: Kang
keywords: Antibacterial activity; Bacterial wilt disease of tomato; Defense genes; Spent mushroom substrate; Suppression