title: Differential effect of cholesterol on type I and II feline coronavirus infection.
abstract: Feline infectious peritonitis (FIP) is a fatal disease of domestic and wild felidae that is caused by feline coronavirus (FCoV). FCoV has been classified into types I and II. Since type I FCoV infection is dominant in the field, it is necessary to develop antiviral agents and vaccines against type I FCoV infection. However, few studies have been conducted on type I FCoV. Here, we compare the effects of cholesterol on types I and II FCoV infections. When cells were treated methyl-β-cyclodextrin (MβCD) and inoculated with type I FCoV, the infection rate decreased significantly, and the addition of exogenous cholesterol to MβCD-treated cells resulted in the recovery of the infectivity of type I FCoV. Furthermore, exogenous cholesterol increased the infectivity of type I FCoV. In contrast, the addition of MβCD and exogenous cholesterol had little effect on the efficiency of type II FCoV infection. These results strongly suggest that the dependence of infection by types I and II FCoV on cholesterol differs.
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takano
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Satomi
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Oyama
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan.
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Doki
authors.affiliation: Laboratory of Veterinary Infectious Disease, School of Veterinary Medicine, Kitasato University, Towada, Japan. hohdatsu@vmas.kitasato-u.ac.jp.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hohdatsu
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000378:metabolism* / Q000821:virology; D002415:Cats; D002784:Cholesterol / Q000378:metabolism*; D018352:Coronavirus Infections / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D016765:Coronavirus, Feline / Q000235:genetics / Q000502:physiology*
chemical_list: D002784:Cholesterol