title: [RNAi-mediated Silencing of CXCR4 Inhibits the Adhesion, Invasion and Tumorigenicity of Acute Monocytic Leukemic Cell Line SHI-1].
abstract: OBJECTIVE
  To investigate the effect of CXCR4 gene on the proliferation, adhesion, invasion and tumorigenicity of a human monocytic leukemic cell line SHI-1.
  
  
  METHODS
  The lentivirus vector silencing the expression of CXCR4 was constructed for infection of SHI-1 cells silencing expression of CXCR4 in SHI-1 cells. The expression of CXCR4, MMP-2 and MMP-9 was detected by real time PCR. The expression of CXCR4 on membrane of SHI-1 cells was detected by flow cytometry. The SHI-1 cell proliferation ability was detected by MTT. The co-culture system of the leukemia cells and bone marrow stromal cells was used to detect the adhesion and migration ability of leukemia cells. SHI-1 cells were inoculated subcutaneously in nude mice to investigate the growth ability in vivo.
  
  
  RESULTS
  After SHI-1 cells were infected by lentivirus silencing expression of CXCR4, the expression of CXCR4 mRNA in SHI-1 CXCR-4i cells decreased by 76% as compared with expression of SHI-1/NC of negative control virus, the expression of CXCR4 on membrane of SHI-1/CXCR4i obviously downregulated; the expression of MMP-2 and MMP-9 in SHI-1/CXCRi cells also declined by 63% and 62% respectively; the proliferation ability of SHI-1/CXCR4i in vitro did not obviously changed, but the adhesion and trans-matrigel invasion ability significantly decreased, the SHI-1/CXCR4i cells could not form neoplasm subcutaneously in mice, but the SHI-1 and SHI-1/NC cells could form neoplasm subcutaneously in mice, and there was no significant difference in volumn of neoplasm mass.
  
  
  CONCLUSIONS
  The silencing expression of CXCR4 can decline the adhesion and migration ability of SHI-1 cells, and can completely suppress the formation of neoplasm subcutaneously, so the CXCR4 may serve as a target for treating leukemia.
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.|Department of Hematology, The Second Hospital Affiliated to Bengbu Medical College, Bengbu 233040, Anhui Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fu
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China. E-mail: lzjdgh@163.com.
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.
authors.forename: Gui-Ling
authors.initials: GL
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.
authors.forename: Ji-Fu
authors.initials: JF
authors.lastname: Zheng
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.
authors.forename: Qing-Zhi
authors.initials: QZ
authors.lastname: Shi
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.
authors.forename: San-Jun
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Second Hospital Affiliated to Nanchang University, Jiangxi Province Key Labratory of Hematology, Nanchang 330006, Jiangxi Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D006801:Humans; D016086:Lentivirus; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D059630:Mesenchymal Stem Cells; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D012333:RNA, Messenger; D019718:Receptors, CXCR4 / Q000235:genetics*; D015398:Signal Transduction
chemical_list: C527276:CXCR4 protein, human; D012333:RNA, Messenger; D019718:Receptors, CXCR4; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9