title: Antioxidant Hydroxytyrosol-Based Polyacrylate with Antimicrobial and Antiadhesive Activity Versus Staphylococcus Epidermidis.
abstract: The accumulation of reactive oxygen species (ROS) in microbial biofilms has been recently recognized to play a role in promoting antibiotic resistance in biofilm-growing bacteria. ROS are also over-produced when a medical device is implanted and they can promote device susceptibility to infection or aseptic loosening. High levels of ROS seem also to be responsible for the establishment of chronic wounds.In this study, a novel antioxidant polyacrylate was synthesized and investigated in terms of antimicrobial and antibiofilm activity. The polymer possesses in side-chain hydroxytyrosol (HTy), that is a polyphenolic compound extracted from olive oil wastewaters.The obtained 60 nm in size polymer nanoparticles showed good scavenging and antibacterial activity versus a strain of Staphylococcus epidermidis. Microbial adherence assays evidenced that the hydroxytyrosol-containing polymer was able to significantly reduce bacterial adhesion compared to the control. These findings open novel perspective for a successful use of this antioxidant polymer for the prevention or treatment of biofilm-based infections as those related to medical devices or chronic wounds.
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Rome, Italy.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Crisante
authors.affiliation: School of Pharmacy, University of Nottingham, Nottingham, UK.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Taresco
authors.affiliation: Microbial Biofilm Laboratory, Fondazione Santa Lucia IRCCS, Rome, Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Donelli
authors.affiliation: Microbial Biofilm Laboratory, Fondazione Santa Lucia IRCCS, Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Vuotto
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Martinelli
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Rome, Italy.
authors.forename: Lucio
authors.initials: L
authors.lastname: D'Ilario
authors.affiliation: ENEA-UTTAMB, C. R. Casaccia, Rome, Italy.
authors.forename: Loris
authors.initials: L
authors.lastname: Pietrelli
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Rome, Italy. iolanda.francolini@uniroma1.it.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Francolini
authors.affiliation: Department of Chemistry, Sapienza University of Rome, Rome, Italy. antonella.piozzi@uniroma1.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Piozzi
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; C005975:3,4-dihydroxyphenylethanol; D010626:Phenylethyl Alcohol
keywords: Antioxidant polymers; Hydroxytyrosol; Medical device-related infections; Microbial biofilm; Nanoparticles