title: Glaucumolides A and B, Biscembranoids with New Structural Type from a Cultured Soft Coral Sarcophyton glaucum.
abstract: Glaucumolides A (1) and B (2), novel biscembranes composed of an unprecedented α,β-unsaturated ε-lactone, along with the known metabolites ximaolide A (3) and isosarcophytonolide D (4), were isolated from the cultured soft coral Sarcophyton glaucum. The structures of the new metabolites were determined by extensive spectroscopic analyses. Compounds 1 and 2 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. In anti-inflammation assay, compounds 1 and 2 displayed strong inhibition of superoxide anion generation and elastase release in human neutrophils stimulated by fMLP/CB. Furthermore, both 1 and 2 were shown to significantly inhibit the accumulation of the pro-inflammatory inducible nitric oxide synthase protein, and compounds 1-3 were found to effectively reduce the expression of cyclooxygenase-2 protein, in lipopolysaccharide-stimulated RAW264.7 macrophage cells.
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Huang
authors.affiliation: National Museum of Marine Biology &Aquarium, Pingtung 944, Taiwan.|Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.
authors.forename: Ping-Jyun
authors.initials: PJ
authors.lastname: Sung
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|National Museum of Marine Biology &Aquarium, Pingtung 944, Taiwan.
authors.forename: Chokkalingam
authors.initials: C
authors.lastname: Uvarani
authors.affiliation: National Museum of Marine Biology &Aquarium, Pingtung 944, Taiwan.|Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.affiliation: National Museum of Marine Biology &Aquarium, Pingtung 944, Taiwan.|Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lu
authors.affiliation: Graduate Institute of Natural Products, School of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan.
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.affiliation: Institute of Oceanography, National Taiwan University, Taipei 112, Taiwan.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Dai
authors.affiliation: General Study Center, National Kaohsiung Marine University, Kaohsiung 805, Taiwan.
authors.forename: Shwu-Li
authors.initials: SL
authors.lastname: Wu
authors.affiliation: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.|Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Jyh-Horng
authors.initials: JH
authors.lastname: Sheu
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000378:metabolism; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000378:metabolism; D003571:Cytochalasin B / Q000009:adverse effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D004224:Diterpenes; D007783:Lactones; D008070:Lipopolysaccharides; C000611705:glaucumolide A; C000611706:glaucumolide B; D013481:Superoxides; D003571:Cytochalasin B; D052247:Nitric Oxide Synthase Type II; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2