title: Zero order and signal processing spectrophotometric techniques applied for resolving interference of metronidazole with ciprofloxacin in their pharmaceutical dosage form.
abstract: Four rapid, simple, accurate and precise spectrophotometric methods were used for the determination of ciprofloxacin in the presence of metronidazole as interference. The methods under study are area under the curve, simultaneous equation in addition to smart signal processing techniques of manipulating ratio spectra namely Savitsky-Golay filters and continuous wavelet transform. All the methods were validated according to the ICH guidelines where accuracy, precision and repeatability were found to be within the acceptable limits. The selectivity of the proposed methods was tested using laboratory prepared mixtures and assessed by applying the standard addition technique. So, they can therefore be used for the routine analysis of ciprofloxacin in quality-control laboratories.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
authors.forename: Khalid A M
authors.initials: KAM
authors.lastname: Attia
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt.
authors.forename: Mohammed W I
authors.initials: MWI
authors.lastname: Nassar
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582 Al Hadaba Al Wosta, Cairo, Egypt.
authors.forename: Mohamed B
authors.initials: MB
authors.lastname: El-Zeiny
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751 Nasr City, Cairo, Egypt. Electronic address: Ahmedserag777@hotmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Serag
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D000890:Anti-Infective Agents / Q000032:analysis*; D002939:Ciprofloxacin / Q000032:analysis*; D057230:Limit of Detection; D008795:Metronidazole / Q000032:analysis*; D013053:Spectrophotometry / Q000191:economics / Q000379:methods*; D058067:Wavelet Analysis
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D008795:Metronidazole; D002939:Ciprofloxacin
keywords: Area under the curve; Ciprofloxacin; Continuous wavelet transform; Metronidazole; Savitsky–Golay filters; Simultaneous equation