title: Influence of gossypol acetic acid on the growth of human adenoid cystic carcinoma ACC-M cells and the expression of DNA methyltransferase 1.
abstract: We investigated the effects of gossypol acetic acid (GAA) on the proliferation, apoptosis, and expression of DNA methyltransferase 1 (DNMT1) mRNA in human adenoid cystic carcinoma (ACC-M) cells in vitro. The proliferation and apoptosis of ACC-M cells after treatment with different concentrations of GAA were detected using Cell Counting Kit-8 and flow cytometry, respectively. DNMT1 mRNA expression was measured by real-time fluorescence quantitative polymerase chain reaction. The growth of ACC-M cells was inhibited after treatment with GAA for 24, 48, and 72 h. The apoptotic rates of ACC-M cells after treatment with GAA for 72 h were higher than those of control cells (without treatment) (P < 0.05). DNMT1 mRNA expression in ACC-M after treatment with GAA for 72 h was lower than that in control cells (P < 0.05). GAA had inhibitory effects on the proliferation and induced apoptosis of human ACC-M cells, while GAA also reduced the expression level of DNMT1 mRNA in ACC-M cells.
authors.affiliation: Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming Yunnan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming Yunnan, China.
authors.forename: Y-N
authors.initials: YN
authors.lastname: Wei
authors.affiliation: Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming Yunnan, China.
authors.forename: W-Y
authors.initials: WY
authors.lastname: Yue
authors.affiliation: Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming Yunnan, China.
authors.forename: W-F
authors.initials: WF
authors.lastname: Chen
authors.affiliation: Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming Yunnan, China.
authors.forename: S
authors.initials: S
authors.lastname: Fu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human; D006072:Gossypol; C028178:gossypol acetic acid