title: [Effect of Alantolactone on Proliferation of RPMI-8226 Cells and Its Possible Mechnism].
abstract: OBJECTIVE
  To investigate the effect of alantolactone on perliferation and apoptosis of multiple myeloma (MM) RPMI-8226 cells, and to explore its possible mechism in vitro and in vivo.
  
  
  METHODS
  The RPMI-8226 cells were treated with alantolactone (1, 2.5, 5, 7.5 and 10 µmol/L) for 48 h, cell viability was detected by CCK-8 assay and the value of IC50 was calculated; The RPMI-8226 cells were treated with alantolactone (2.5, 5 and 7.5 µmol/L) for 48 h, the apoptotic rate was detected by flow cytmetry with Annexin V/PI staining; the expression level of cleaved caspase-3 and phosphorylation of ERK were measured by Western blot; the nude mice was used to further confirm the proapoptotic effect of alantolactone on MM cells in vivo.
  
  
  RESULTS
  The alantolactone inhibited RPMI-8226 cell viability remarkably with a dose-dependent manner; the IC50 value of RPMI-8226 cells at 48 h was 4.32 ± 0.15 µmol/L; the apoptotic rate increased observably with a dose-dependent manner; the levels of cleaved-caspase-3 increased and the phosphorylation of ERK decreased significantly; as compared to control, the volum of tumor was much smaller, the expression levels of Ki67 and p-ERK decreased.
  
  
  CONCLUSIONS
  The alantolactone can efficiently inhibit the proliferation and induce the apoptosis of multiple myeloma RPMI-8226 cells in vitro and in vivo through inhibiting the activation of ERK signal pathway.
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Yue-Yue
authors.initials: YY
authors.lastname: Sun
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Xia
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Ming-Shan
authors.initials: MS
authors.lastname: Niu
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Li
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006, Jiangsu Province, China. E-mail: kailinxu_xzmc@126.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D009101:Multiple Myeloma / Q000473:pathology*; D045787:Sesquiterpenes, Eudesmane / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D007783:Lactones; D045787:Sesquiterpenes, Eudesmane; C505407:CASP3 protein, human; D053148:Caspase 3; C004363:alantolactone