title: Cellularized Cellular Solids via Freeze-Casting.
abstract: The elaboration of metabolically active cell-containing materials is a decisive step toward the successful application of cell based technologies. The present work unveils a new process allowing to simultaneously encapsulate living cells and shaping cell-containing materials into solid-state macroporous foams with precisely controlled morphology. Our strategy is based on freeze casting, an ice templating materials processing technique that has recently emerged for the structuration of colloids into macroporous materials. Our results indicate that it is possible to combine the precise structuration of the materials with cellular metabolic activity for the model organism Saccharomyces cerevisiae.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), 11 Place Marcelin Berthelot, F-75005, Paris, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: Christoph
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), 11 Place Marcelin Berthelot, F-75005, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Kwiatoszynski
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), 11 Place Marcelin Berthelot, F-75005, Paris, France.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), 11 Place Marcelin Berthelot, F-75005, Paris, France. francisco.fernandes@upmc.fr.
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Fernandes
mesh_terms: D000464:Alginates / Q000737:chemistry*; D005615:Freezing; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D016062:Porosity; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid
keywords: Encapsulation; Saccharomyces cerevisiae; freeze casting; ice templating; macroporous foams