title: Extracellular matrix metalloproteinase inducer (EMMPRIN) remodels the extracellular matrix through enhancing matrix metalloproteinases (MMPs) and inhibiting tissue inhibitors of MMPs expression in HPV-positive cervical cancer cells.
abstract: PUPOSE OF INVESTIGATION: To study the expression of extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinases (MMPs), and tissue inhibitors of MMP (TIMPs) in uterine cervical cancer cell lines in vitro.
  
  
  METHODS
  EMMPRIN, MMPs, and TIMPs expression were assessed by Western blot and real-time RT-PCR from cervical carcinoma SiHa, HeLa, and C33-A cells.
  
  
  RESULTS
  EMMPRIN recombinant significantly increased MMP-2, MMP-9 protein and mRNA expression in SiHa and Hela cells, but not in C33-A cells by Western blot analysis and real-time RT-PCR. EMMPRIN recombinant significantly inhibited TIMP-1 protein and mRNA levels in SiHa and Hela cells, but not in C33-A cells. There was no difference on the TIMP-2 expression in those cells with the treatment of EMMPRIN recombinant. EMMPRIN RNAi decreased MMP-2 and MMP-9 and increased TIMP-1 expression in SiHa and HeLa cells, but not in C33-A cells. There was no change on the expression of TIMP-2 mRNA levels in SiHa, HeLa and C33-A cells transfected with siEMMPRIN.
  
  
  CONCLUSIONS
  EMMPRIN may induce MMP-2 and MMP-9, and downregulate TIMP-1 in HPV-positive cervical cancer cells in vitro.
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: Y
authors.initials: Y
authors.lastname: Ye
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: N
authors.initials: N
authors.lastname: Ohara
authors.forename: H
authors.initials: H
authors.lastname: Ji
mesh_terms: D051926:Basigin / Q000502:physiology*; D045744:Cell Line, Tumor; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D006801:Humans; D020782:Matrix Metalloproteinases / Q000096:biosynthesis* / Q000235:genetics; D027383:Papillomaviridae / Q000302:isolation & purification*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: C497886:BSG protein, human; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D051926:Basigin; D020782:Matrix Metalloproteinases