title: Controlling transcription in human pluripotent stem cells using CRISPR-effectors.
abstract: The ability to manipulate transcription in human pluripotent stem cells (hPSCs) is fundamental for the discovery of key genes and mechanisms governing cellular state and differentiation. Recently developed CRISPR-effector systems provide a systematic approach to rapidly test gene function in mammalian cells, including hPSCs. In this review, we discuss recent advances in CRISPR-effector technologies that have been employed to control transcription through gene activation, gene repression, and epigenome engineering. We describe an application of CRISPR-effector mediated transcriptional regulation in hPSCs by targeting a synthetic promoter driving a GFP transgene, demonstrating the ease and effectiveness of CRISPR-effector mediated transcriptional regulation in hPSCs.
authors.affiliation: Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Ryan M
authors.initials: RM
authors.lastname: Genga
authors.affiliation: Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA.
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Kearns
authors.affiliation: Program in Molecular Medicine, Diabetes Center of Excellence, University of Massachusetts Medical School, Worcester, MA 01605, USA. Electronic address: rene.maehr@umassmed.edu.
authors.forename: René
authors.initials: R
authors.lastname: Maehr
mesh_terms: D000818:Animals; D001483:Base Sequence; D064113:CRISPR-Cas Systems; D018929:Cell Culture Techniques; D057809:HEK293 Cells; D006801:Humans; D039904:Pluripotent Stem Cells / Q000502:physiology*; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000094704:RNA, Guide, CRISPR-Cas Systems
keywords: CRISPR; Cas9; Human pluripotent stem cells; Transcriptional regulation; dCas9-effectors