title: Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
abstract: Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass. In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible. Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation. In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation. Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed. The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively. Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage. In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis. Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found. In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Chunliang
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Zuohua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Zhenxiu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
authors.forename: Yuande
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000681:Amylases / Q000032:analysis / Q000378:metabolism; D018533:Biomass; D032402:Boehmeria / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; C007916:hemicellulose; D010368:Pectins; D002482:Cellulose; D008031:Lignin; D010744:Phosphoric Monoester Hydrolases; D000681:Amylases; D010447:Peptide Hydrolases
keywords: Lignocellulose enzyme; P. eryngii; Ramie stalk; Secretome