title: Co-silencing of human Bub3 and dynein highlights an antagonistic relationship in regulating kinetochore-microtubule attachments.
abstract: We previously reported that the spindle assembly checkpoint protein Bub3 is involved in regulating kinetochore-microtubule (KT-MT) attachments. Also, Bub3 was reported to interact with the microtubule motor protein dynein. Here we examined how this interaction contributes to KT-MT attachments. Depletion of Bub3 or dynein induced misaligned chromosomes, consistent with their role in KT-MT attachments. Unexpectedly, co-silencing of both proteins partially suppressed the misalignment phenotype and restored chromosome congression. Consistent with these observations, KT-MT attachments in co-depleted cells were stable, able to drive chromosome congression, and produce inter- and intra-kinetochore stretch, indicating they are functional. We suggest that a mutual antagonism exists between Bub3 and dynein to ensure optimal KT-MT attachments.
authors.affiliation: CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116 Gandra PRD, Portugal; Centre for Biomedical Research (CBMR), University of Algarve, Faro 8005-139, Portugal.
authors.forename: Patrícia M A
authors.initials: PM
authors.lastname: Silva
authors.affiliation: Centre for Biomedical Research (CBMR), University of Algarve, Faro 8005-139, Portugal; Departamento Ciências Biomédicas e Medicina, University of Algarve, Faro 8005-139, Portugal.
authors.forename: Álvaro A
authors.initials: ÁA
authors.lastname: Tavares
authors.affiliation: CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra, 1317, 4585-116 Gandra PRD, Portugal; Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Universidade do Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal. Electronic address: hassan.bousbaa@iscsn.cespu.pt.
authors.forename: Hassan
authors.initials: H
authors.lastname: Bousbaa
mesh_terms: D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors* / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D002869:Chromosome Aberrations; D004398:Dyneins / Q000037:antagonists & inhibitors* / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D010641:Phenotype; D000075223:Poly-ADP-Ribose Binding Proteins
chemical_list: C489444:BUB3 protein, human; D018797:Cell Cycle Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D004398:Dyneins
keywords: Antagonistic interaction; Bub3; Chromosome congression; Dynein; Kinetochore–microtubule interaction; Spindly