title: High-throughput platforms for metabolomics.
abstract: Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we highlight current high-throughput MS-based platforms and their potential application in metabolomics. Although current MS platforms can reach throughputs up to 0.5 seconds per sample, the metabolite coverage of these platforms are low compared to low-throughput, separation-based MS methods. High-throughput comes at a cost, as it's a trade-off between sample throughput and metabolite coverage. As we will discuss, promising emerging technologies, including microfluidics and miniaturization of separation techniques, have the potential to achieve both rapid and more comprehensive metabolite analysis.
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, United States.
authors.forename: Markus
authors.initials: M
authors.lastname: de Raad
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, United States.
authors.forename: Curt R
authors.initials: CR
authors.lastname: Fischer
authors.affiliation: Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, United States. Electronic address: trnorthen@lbl.gov.
authors.forename: Trent R
authors.initials: TR
authors.lastname: Northen
mesh_terms: D006801:Humans; D056656:Lab-On-A-Chip Devices; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*