title: Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose.
abstract: BACKGROUND
  Endophytic bacteria are viewed as a potential new source of biofungicides because they have beneficial characteristics as control agents for plant disease. This study was performed to examine the endophytic feature and disease control efficacy of Bacillus amyloliquefaciens strain GYL4 and to identify the antifungal compounds produced by this strain.
  
  
  RESULTS
  B. amyloliquefaciens strain GYL4 was isolated from leaf tissue of pepper plants (Capsicum annuum L.). Anthracnose symptoms were markedly reduced in the leaves of pepper plants colonised by GYL4. An egfp-expressing strain of GYL4 (GYL4-egfp) was constructed and reintroduced into pepper plants, which confirmed its ability to colonise the internal tissues of pepper plants. GYL4-egfp was observed in the root and stem tissues 4 days after treatment and abundantly found in the internal leaf tissue 9 days after treatment. Bacillomycin derivatives purified from the culture extract of GYL4 displayed control efficacy on anthracnose development in cucumber (Cucumis sativus L. cv. Chunsim).
  
  
  CONCLUSIONS
  The present study is the first report on evaluation of the endophytic and systemic nature of B. amyloliquefaciens strain GYL4 and its potential as a biocontrol agent for anthracnose management. © 2015 Society of Chemical Industry.
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Jeong Do
authors.initials: JD
authors.lastname: Kim
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Byeong Jun
authors.initials: BJ
authors.lastname: Jeon
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Han
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Park
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Sin Ae
authors.initials: SA
authors.lastname: Kang
authors.affiliation: Laboratory of Plant Pharmacology, Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea.
authors.forename: Beom Seok
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000069977:Bacillus amyloliquefaciens / Q000737:chemistry* / Q000378:metabolism; D061046:Biological Control Agents / Q000737:chemistry* / Q000378:metabolism; D002212:Capsicum / Q000382:microbiology*; D018553:Cucumis sativus / Q000382:microbiology; D060026:Endophytes / Q000737:chemistry / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control
chemical_list: D061046:Biological Control Agents
keywords: Bacillus amyloliquefaciens; anthracnose; bacillomycin D; biofungicide; endophytic bacteria; systemic colonisation