title: New type of pathogenicity of Thelohanellus kitauei Egusa & Nakajima, 1981 infecting the skin of common carp Cyprinus carpio L.
abstract: Thelohanellus kitauei Egusa & Nakajima, 1981 is a common parasite infecting the intestine of common carp Cyprinus carpio L., resulting in mass mortality or loss of economic value of cultured carp. In the present study, T. kitauei infecting host skin was detected. The morphological, molecular and histological data of this parasite in the new organ record are presented. Morphological analysis showed the current specimen morphologically similar to T. kitauei from the intestine. Despite the spore length and polar capsule length of the current specimen larger than those of T. kitauei from the intestine, ranges of dimensions overlap, which is more suggestive of intraspecific variation than distinct species. BLAST search revealed that the present small subunit ribosomal DNA gene sequence is identical to those of T. kitauei. Histologically, most of spores distributed in the stratum spongiosum of dermis, and some spores in the strata compactum of host skin were also observed. Above all, both morphology and molecular analysis indicated that the current species from the skin of common carp is conspecific with T. kitauei from the intestine of carp and organ habitats transfer of T. kitauei from host intestine to skin may have occurred.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, People's Republic of China, 430070; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, People's Republic of China, 430070.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, People's Republic of China, 430070; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, People's Republic of China, 430070.
authors.forename: Zemao
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070.
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, People's Republic of China, 430070; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, People's Republic of China, 430070; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, People's Republic of China, 430070. Electronic address: liuyangqzh@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000469:parasitology*; D005393:Fish Diseases / Q000469:parasitology*; D007422:Intestines / Q000469:parasitology / Q000473:pathology; D055764:Myxozoa / Q000033:anatomy & histology / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA / Q000379:methods; D012867:Skin / Q000469:parasitology* / Q000473:pathology; D012876:Skin Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary*; D013170:Spores / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure; D014774:Virulence
keywords: Common carp; Myxosporea; Thelohanellus kitauei; ssrDNA