title: A mitochondria-targeted ratiometric two-photon fluorescent probe for biological zinc ions detection.
abstract: A mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MPVQ) for biological zinc ions detection was developed based on quinolone platform. Mito-MPVQ showed large red shifts (68 nm) and selective ratiometric signal upon Zn(2+) binding. The ratio of emission intensity (I488 nm/I420 nm) increases dramatically from 0.45 to 3.79 (ca. 8-fold). NMR titration and theoretical calculation confirmed the binding of Mito-MPVQ and Zn(2+). Mito-MPVQ also exhibited large two-photon absorption cross sections (150 GM) at nearly 720 nm and insensitivity to pH within the biologically relevant pH range. Cell imaging indicated that Mito-MPVQ could efficiently located in mitochondria and monitor mitochondrial Zn(2+) under two-photon excitation with low cytotoxicity.
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ning
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Longchun
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Haizhu
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China.
authors.forename: Manzhou
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei 230601, China.
authors.forename: Buchang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Biochemistry, BioFrontiers Institute, University of Colorado at Boulder, Boulder, CO 80309, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Chemistry, Anhui University, Hefei 230601, China; Center for Atomic Engineering of Advanced Material, Anhui University, Hefei 230601, China. Electronic address: mengxm@ahu.edu.cn.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D016466:CHO Cells; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure*; D057054:Molecular Imaging / Q000379:methods*; D015336:Molecular Probe Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D015032:Zinc / Q000032:analysis / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D015032:Zinc
keywords: Fluorescent probe; Mitochondria-targeted; Ratiometric; Two-photon; Zinc ions