title: Bacillus cereus AR156 primes induced systemic resistance by suppressing miR825/825* and activating defense-related genes in Arabidopsis.
abstract: Small RNAs play an important role in plant immune responses. However, their regulatory function in induced systemic resistance (ISR) is nascent. Bacillus cereus AR156 is a plant growth-promoting rhizobacterium that induces ISR in Arabidopsis against bacterial infection. Here, by comparing small RNA profiles of Pseudomonas syringae pv. tomato (Pst) DC3000-infected Arabidopsis with and without AR156 pretreatment, we identified a group of Arabidopsis microRNAs (miRNAs) that are differentially regulated by AR156 pretreatment. miR825 and miR825* are two miRNA generated from a single miRNA gene. Northern blot analysis indicated that they were significantly downregulated in Pst DC3000-infected plants pretreated with AR156, in contrast to the plants without AR156 pretreatment. miR825 targets two ubiquitin-protein ligases, while miR825* targets toll-interleukin-like receptor (TIR)-nucleotide binding site (NBS) and leucine-rich repeat (LRR) type resistance (R) genes. The expression of these target genes negatively correlated with the expression of miR825 and miR825*. Moreover, transgenic plants showing reduced expression of miR825 and miR825* displayed enhanced resistance to Pst DC3000 infection, whereas transgenic plants overexpressing miR825 and miR825* were more susceptible. Taken together, our data indicates that Bacillus cereus AR156 pretreatment primes ISR to Pst infection by suppressing miR825 and miR825* and activating the defense related genes they targeted.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.|Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, California, CA-92521, USA.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Niu
authors.affiliation: Department of Computer Science and Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA.|The Institute for Systems Biology, Jianghan University, Wuhan 430056, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xia
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Chunhao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Qi
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Ling
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Lin
authors.affiliation: Department of Computer Science and Engineering, Washington University in St Louis, St Louis, Missouri 63130, USA.
authors.forename: Weixiong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Plant Pathology and Microbiology, Center for Plant Cell Biology and Institute for Integrative Genome Biology, University of California, Riverside, California, CA-92521, USA.
authors.forename: Hailing
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agriculture University, Nanjing, 210095, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D001409:Bacillus cereus / Q000502:physiology*; D001483:Base Sequence; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D017343:Genes, Plant; D056426:Genetic Loci; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000502:physiology; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: C000613157:MIRN825 microRNA, Arabidopsis; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: ISR; Induced systemic resistance; microRNA; plant innate immunity; small RNA