title: Effect of biological behavior and clinical significance of maspin gene on cervical squamous carcinoma SiHa cell.
abstract: OBJECTIVE
  This study was performed to evaluate the effect of mammary serine protease inhibitor (maspin) overexpression on human cervical squamous carcinoma (SCC) SiHa cell proliferation and apoptosis in vitro.
  
  
  METHODS
  Recombinant plasmid pcDNA3-maspin was stably transfected into human cervical SCC SiHa cell. Maspin mRNA was determined by RT-PCR, whereas maspin protein was detected by Western blot analysis and immunocytochemistry (IHC). Cell proliferation activity was measured by MTT method. Apoptosis rate and cell cycle distribution were detected by flow cytometry to understand the changes in the cell biological characteristics.
  
  
  RESULTS
  The strengthened expression of the maspin gene in the SiHa cell was confirmed by RT-PCR, Western blot, and immunocytochemistry (IHC) (p < 0.05). Suppressed proliferation activity and increased apoptosis rate of SiHa-m (maspin stable transfected) versus SiHa and SiHa-vector cell (SiHa-pc3) were shown by MTT and flow cytometry (p < 0.05). SiHa and SiHa-pc3-had no statistical significance (p > 0.05).
  
  
  CONCLUSIONS
  The results showed that maspin gene can significantly inhibit human cervical SCC SiHa cell proliferation and effectively slow cancer growth. Maspin may be a new molecular target in the gene therapy of human cervical SCC.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Liu
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhao
authors.forename: W
authors.initials: W
authors.lastname: Meng
authors.forename: G Q
authors.initials: GQ
authors.lastname: Zhi
authors.forename: F F
authors.initials: FF
authors.lastname: Nan
authors.forename: D H
authors.initials: DH
authors.lastname: Zhao
authors.forename: Z I
authors.initials: ZI
authors.lastname: Peng
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D015843:Serpins / Q000235:genetics* / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy
chemical_list: C085344:SERPIN-B5; D015843:Serpins