title: Method Modification of the Thermo Scientific SureTect Listeria monocytogenes Assay for Raw Meat, Dairy, Produce, and Seafood.
abstract: The Thermo Scientific™ SureTect™ Listeria monocytogenes assay is a real-time PCR assay for the detection of Listeria monocytogenes in food and environmental samples, which was certified during 2013 by the AOAC Research Institute (RI) as Performance Tested Method(SM) (PTM) 061302 for a representative range of key food matrixes and production surfaces. This report details the method modification study, which was conducted during 2014, using the AOAC-RI PTM program to extend the validated matrix claims of the assay in comparison to the reference method detailed in International Organization for Standardization 11290-1:1996, including Amendment 1:2004, to gain certification for raw ground turkey, raw ground pork, pasteurized 2% milk, raw pork sausages, raw cod, pasteurized brie cheese, cooked sliced ham, and bagged lettuce. All matrixes were tested by Thermo Fisher Scientific, Microbiology Division, Basingstoke, UK. In addition, brie cheese, bagged lettuce, and raw cod were analyzed independently by the University of Guelph, Canada, during the AOAC-RI controlled independent laboratory study. Using probability of detection (POD) statistical analysis, a significant difference was demonstrated between the candidate and reference methods for the high spiking level with raw ground pork and brie cheese. For all other matrixes and the low spiked levels for raw ground pork and brie cheese, no significant difference by POD was seen between the two methods during the study.
authors.affiliation: Thermo Fisher Scientific, Wade Rd, Basingstoke, Hampshire, RG24 8PW, UK.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cloke
authors.forename: Katharine
authors.initials: K
authors.lastname: Evans
authors.forename: David
authors.initials: D
authors.lastname: Crabtree
authors.forename: Annette
authors.initials: A
authors.lastname: Hughes
authors.forename: Helen
authors.initials: H
authors.lastname: Simpson
authors.forename: Carlos
authors.initials: C
authors.lastname: Leon-Velarde
authors.forename: Nathan
authors.initials: N
authors.lastname: Larson
authors.forename: Keron
authors.initials: K
authors.lastname: Dave
authors.forename: Jani
authors.initials: J
authors.lastname: Holopainen
authors.forename: Nina
authors.initials: N
authors.lastname: Wickstrand
authors.forename: Mikko
authors.initials: M
authors.lastname: Kauppinen
mesh_terms: D000818:Animals; D002417:Cattle; D003611:Dairy Products / Q000382:microbiology*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics*; D008460:Meat / Q000032:analysis*; D066256:Raw Foods / Q000032:analysis* / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D017747:Seafood / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic