title: The Fungal Biome of the Oral Cavity.
abstract: Organisms residing in the oral cavity (oral microbiota) contribute to health and disease, and influence diseases like gingivitis, periodontitis, and oral candidiasis (the most common oral complication of HIV-infection). These organisms are also associated with cancer and other systemic diseases including upper respiratory infections. There is limited knowledge regarding how oral microbes interact together and influence the host immune system. Characterizing the oral microbial community (oral microbiota) in health and disease represents a critical step in gaining insight into various members of this community. While most of the studies characterizing oral microbiota have focused on bacterial community, there are few encouraging studies characterizing the oral mycobiome (the fungal component of the oral microbiota). Our group recently characterized the oral mycobiome in health and disease focusing on HIV. In this chapter we will describe the methods used by our group for characterization of the oral mycobiome.
authors.affiliation: Center for Medical Mycology and Department of Dermatology, University Hospitals Case Medical Center and Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH, 44106, USA.
authors.forename: Jyotsna
authors.initials: J
authors.lastname: Chandra
authors.affiliation: Center for Medical Mycology and Department of Dermatology, University Hospitals Case Medical Center and Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH, 44106, USA.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Retuerto
authors.affiliation: Center for Medical Mycology and Department of Dermatology, University Hospitals Case Medical Center and Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH, 44106, USA.
authors.forename: Pranab K
authors.initials: PK
authors.lastname: Mukherjee
authors.affiliation: Center for Medical Mycology and Department of Dermatology, University Hospitals Case Medical Center and Case Western Reserve University, 11100 Euclid Ave, Cleveland, OH, 44106, USA. Mahmoud.Ghannoum@case.edu.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Ghannoum
mesh_terms: D004271:DNA, Fungal; D021901:DNA, Intergenic; D005658:Fungi / Q000145:classification / Q000235:genetics; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D064307:Microbiota; D009055:Mouth / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic
keywords: Amplification; Fungal DNA extraction; Fungus; Mycobiome; Oral microbiota; Sequencing