title: Histopathological and genotoxic effects of chlorpyrifos in rats.
abstract: This study aims to investigate the effects of chlorpyrifos's sub-acute exposure on male rats. Two groups with six animals each were orally treated, respectively, with 3.1 mg/kg b w and 6.2 mg/kg b w of chlorpyrifos during 4 weeks. The genotoxic effect of chlopyrifos was investigated using the comet assay and the micronucleus test. Some hematological and liver's histopathological changes were also evaluated. Results revealed that chlorpyrifos induced histopathological alterations in liver parenchyma. The lymphoid infiltration observed in liver sections and the increase in white blood cells parameter are signs of inflammation. A significant increase in the platelet' count and in polychromatic erythrocytes/normochromatic erythrocytes (PCE/NCE) ratio was observed in chlorpyrifos-treated groups which could be due to the stimulatory effect of chlorpyrifos on cell formation in the bone marrow at lower doses. In addition, the increase of bone marrow micronucleus percentage and the comet tail length revealed a genotoxic potential of chlorpyrifos in vivo.
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia. ezzilobna83@gmail.com.
authors.forename: Lobna
authors.initials: L
authors.lastname: Ezzi
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Belhadj Salah
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Zohra
authors.initials: Z
authors.lastname: Haouas
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Amina
authors.initials: A
authors.lastname: Sakly
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Intissar
authors.initials: I
authors.lastname: Grissa
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Sana
authors.initials: S
authors.lastname: Chakroun
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Emna
authors.initials: E
authors.lastname: Kerkeni
authors.affiliation: Laboratory of Hematology, Fattouma Bourguiba University Hospital Monastir, Monastir, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Hassine
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Meriem
authors.initials: M
authors.lastname: Mehdi
authors.affiliation: Laboratory of Histology and Cytogenetic (Research Unit of Genetic, Genotoxicity and Childhood Illness UR12ES10), Faculty of Medicine, University of Monastir, Street Avicenne, Monastir, 5019, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Ben Cheikh
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D004390:Chlorpyrifos / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests; D009929:Organ Size / Q000187:drug effects; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004785:Environmental Pollutants; D004390:Chlorpyrifos
keywords: Chlorpyrifos; Genotoxicity; Hematological parameters; Liver; Organophosphate