title: Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody.
abstract: Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis.
authors.affiliation: INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.|Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.|Université Paris Descartes, UMR S 1138, Paris, France.
authors.forename: Nimesh
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Flavivirus-Host Molecular Interactions laboratory, Virology Department, Institut Pasteur, Paris, France.
authors.forename: Mélissanne
authors.initials: M
authors.lastname: de Wispelaere
authors.affiliation: INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.|Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.|Université Paris Descartes, UMR S 1138, Paris, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Lecerf
authors.affiliation: Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
authors.forename: Manjula
authors.initials: M
authors.lastname: Kalia
authors.affiliation: Deutsches Rheuma-Forschungszentrum, Institut der Leibniz-Gemeinschaft, 10117 Berlin, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Scheel
authors.affiliation: Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, Faridabad, Haryana, India.
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Vrati
authors.affiliation: Deutsches Rheuma-Forschungszentrum, Institut der Leibniz-Gemeinschaft, 10117 Berlin, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Berek
authors.affiliation: INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.|Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.|Université Paris Descartes, UMR S 1138, Paris, France.
authors.forename: Srinivas V
authors.initials: SV
authors.lastname: Kaveri
authors.affiliation: Flavivirus-Host Molecular Interactions laboratory, Virology Department, Institut Pasteur, Paris, France.|Infection and Epidemiology Department, Institut Pasteur, 75724 Paris &UMR PIMIT (I2T), University of Reunion Island, INSERM U1187, CNRS 9192, IRD 249, GIP-CYROI, la Reunion, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Desprès
authors.affiliation: INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.|Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.|Université Paris Descartes, UMR S 1138, Paris, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lacroix-Desmazes
authors.affiliation: INSERM, UMR S 1138, Centre de Recherche des Cordeliers, Paris, France.|Université Pierre et Marie Curie-Paris6, UMR S 1138, Paris, France.|Université Paris Descartes, UMR S 1138, Paris, France.
authors.forename: Jordan D
authors.initials: JD
authors.lastname: Dimitrov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000276:immunology* / Q000472:pathogenicity; D006418:Heme / Q000008:administration & dosage*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007700:Kinetics; D009500:Neutralization Tests; D013816:Thermodynamics; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D006418:Heme