title: Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample.
abstract: A Gram-stain positive, non-motile, non-sporogenous, aerobic, rod-shaped and halophilic bacterium, designated LAM0015(T), was isolated from a saline sediment sample collected from Yantai City in China. The isolate was found to be able to grow at NaCl concentrations of 5-25 % (w/v) (optimum: 7-12 %), 15-45 °C (optimum: 35 °C) and pH 5.0-9.0 (optimum: 7.0). The major fatty acids were determined to be anteiso-C15:0 and anteiso-C17:0. The predominant respiratory quinone was identified as MK-7. The cell wall peptidoglycan was determined to contain meso-diaminopimelic acid. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, five phospholipids and one glycolipid. The DNA G+C content was 43.1 mol% as determined by the T m method. Analysis of the 16S rRNA gene sequence indicated that the isolate belongs within the genus Lentibacillus and is closely related to Lentibacillus persicus DSM 22530(T), Lentibacillus salicampi JCM 11462(T) and Lentibacillus jeotgali JCM 15795(T) with 97.3, 96.7 and 96.4 % sequence similarity, respectively. The DNA-DNA hybridization value between LAM0015(T) and L. persicus DSM 22530(T) was 51.2 ± 1.4 %. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0015(T) is concluded to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus amyloliquefaciens sp. nov. is proposed. The type strain is LAM0015(T) (=ACCC 06401(T) = JCM 19838(T)).
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People's Republic of China.
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Wang
authors.affiliation: College of Environmental and Chemical Engineering, Dalian University, Dalian, 116622, People's Republic of China.
authors.forename: Ke-Dong
authors.initials: KD
authors.lastname: Ma
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People's Republic of China.
authors.forename: Yan-Wei
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Tarim University, and Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources, Tarim Basin, Alaer City, 843300, People's Republic of China.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Chen
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China.
authors.forename: De-Long
authors.initials: DL
authors.lastname: Kong
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People's Republic of China. hemingxiong@caas.cn.
authors.forename: Ming-Xiong
authors.initials: MX
authors.lastname: He
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People's Republic of China. ruanzhiyong@caas.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Ruan
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D019015:Geologic Sediments / Q000032:analysis / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride
keywords: 16S rRNA gene; Halophilic; Lentibacillus amyloliquefaciens sp. nov; Polyphasic taxonomy; Saline sediment