title: NtRING1, putative RING-finger E3 ligase protein, is a positive regulator of the early stages of elicitin-induced HR in tobacco.
abstract: NtRING1 is a RING-finger protein with a putative E3 ligase activity. NtRING1 regulates HR establishment against different pathogens. Loss-/gain-of-function of NtRING1 altered early stages of HR phenotype establishment. Plant defence responses against pathogens often involve the restriction of pathogens by inducing a hypersensitive response (HR). cDNA clones DD11-39, DD38-11 and DD34-26 were previously obtained from a differential screen aimed at characterising tobacco genes with an elicitin-induced HR-specific pattern of expression. Our precedent observations suggested that DD11-39, DD38-11 and DD34-26 might play roles in the HR establishment. Only for DD11-39 a full-length cDNA sequence was obtained and the corresponding protein encoded for a type-HC RING-finger/putative E3 ligase protein which we termed NtRING1. The expression of NtRING1 was upregulated upon HR induction by elicitin, Ralstonia solanacearum, or tobacco mosaic virus (TMV) in tobacco. Silencing of NtRING1 remarkably delayed the establishment of elicitin-induced HR in tobacco as well as the expression of different early induction genes in tissues undergoing HR. Accordingly, transient overexpression of NtRING1 accelerated the HR launching upon elicitin treatment. Taking together, our data suggests that NtRING1 plays a functional role in the early establishment of HR.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg, France. ahmedghannam@hotmail.com.|Laboratory Functional Genomics for Plant Immunomodulation, Plant Pathology Division, Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, P.O. Box 6091, Damascus, Syria. ahmedghannam@hotmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ghannam
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg, France.|Ecole d'ingénieurs de Purpan, Laboratoire d'Agro-Physiologie, 75 voie du TOEC, 31076, Toulouse Cedex 3, France.
authors.forename: Alban
authors.initials: A
authors.lastname: Jacques
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: de Ruffray
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084, Strasbourg, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Kauffmann
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases
keywords: Eilictin; Hypersensitive response (HR); NtRING1; Type-HC RING-finger protein