title: MicroRNA-340 Mediates Metabolic Shift in Oral Squamous Cell Carcinoma by Targeting Glucose Transporter-1.
abstract: OBJECTIVE
  MicroRNA-340 (miR-340) is deregulated in many human cancers in correlation with tumor progression. Recent studies have found that microRNAs play key roles in energy metabolism. This study explored the contributions of miR-340 to the metabolic shift in oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  MiR-340 expression was measured by real-time polymerase chain reaction. MiR-340 mimics, miR-340 inhibitor, and scramble small interfering RNA were transfected into SAS human tongue SCC cells to observe their effects on cell proliferation, colony formation, lactate secretion, and glucose uptake rate. Moreover, the relation between the level of miR-340 and glucose transporter-1 (Glut1) was investigated.
  
  
  RESULTS
  The expression of miR-340 was decreased and thus induced a metabolic switch in oral cancer cells. The decrease in miR-340 increased Glut1 expression, leading to an increase in lactate secretion and glucose uptake rate. The altered metabolism induced by miR-340 resulted in the rapid proliferation of oral cancer cells.
  
  
  CONCLUSIONS
  The findings suggest that miR-340 might act as a molecular switch that contributes to the regulation of glycolysis in OSCC by regulating Glut1 expression.
authors.affiliation: Attending Physician, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: Resident, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Resident, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Haiyuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Resident, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China.
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Li
authors.affiliation: Department Head, Department of Stomatology, Chengdu Military General Hospital, Chengdu, China. Electronic address: hongwei365@yeah.net.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhu
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000502:physiology*; D006019:Glycolysis / Q000502:physiology; D057809:HEK293 Cells; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000502:physiology; D014062:Tongue Neoplasms / Q000378:metabolism*; D014162:Transfection
chemical_list: D051272:Glucose Transporter Type 1; C570046:MIRN340 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D019344:Lactic Acid; D005947:Glucose