title: Expression of annexin A1 in Leishmania-infected skin and its correlation with histopathological features.
abstract: BACKGROUND
  The aim of this study was quantify annexin A1 expression in macrophages and cluster of differentiation 4 (CD4) + and cluster of differentiation 8 (CD8)+ T cells from the skin of patients with cutaneous leishmaniasis (n=55) and correlate with histopathological aspects.
  
  
  METHODS
  Infecting species were identified by polymerase chain reaction-restriction fragment length polymorphism, and expression of annexin A1 was analyzed by immunofluorescence.
  
  
  RESULTS
  All patients (n = 55) were infected with Leishmania braziliensis . Annexin A1 was expressed more abundantly in CD163 + macrophages in infected skin (p < 0.0001) than in uninfected skin. In addition, macrophages in necrotic exudative reaction lesions expressed annexin A1 at higher levels than those observed in granulomatous (p < 0.01) and cellular lesions p < 0.05). This difference might be due to the need to clear both parasites and necrotic tissue from necrotic lesions. CD4 + cells in cellular lesions expressed annexin A1 more abundantly than did those in necrotic (p < 0.05) and granulomatous lesions (p < 0.01). Expression in CD8 + T cells followed the same trend. These differences might be due to the pervasiveness of lymphohistiocytic and plasmacytic infiltrate in cellular lesions.
  
  
  CONCLUSIONS
  Annexin A1 is differentially expressed in CD163 + macrophages and T cells depending on the histopathological features of Leishmania -infected skin, which might affect cell activation.
authors.affiliation: Pós-Graduação em Ciências da Saúde, Laboratório de Histologia, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
authors.forename: Helen Aguiar Lemes da
authors.initials: HA
authors.lastname: Silva
authors.affiliation: Laboratório de Histologia, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
authors.forename: Gabriel Silva de
authors.initials: GS
authors.lastname: Lima
authors.affiliation: Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Mariana Côrtes
authors.initials: MC
authors.lastname: Boité
authors.affiliation: Laboratório de Pesquisa em Leishmaniose, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Porrozzi
authors.affiliation: Hospital Universitário Julio Müller, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
authors.forename: Marcia
authors.initials: M
authors.lastname: Hueb
authors.affiliation: Pós-Graduação em Ciências da Saúde, Laboratório de Histologia, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.
authors.forename: Amilcar Sabino
authors.initials: AS
authors.lastname: Damazo
mesh_terms: D017305:Annexin A1 / Q000032:analysis / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D003430:Cross-Sectional Studies; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007891:Leishmania / Q000145:classification*; D016773:Leishmaniasis, Cutaneous / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017305:Annexin A1