title: Knockout of the Host Resistance Gene Pkr Fully Restores Replication of Murine Cytomegalovirus m142 and m143 Mutants In Vivo.
abstract: Murine cytomegalovirus (MCMV) proteins m142 and m143 are essential for viral replication. They bind double-stranded RNA and prevent protein kinase R-induced protein synthesis shutoff. Whether the two viral proteins have additional functions such as their homologs in human cytomegalovirus do remained unknown. We show that MCMV m142 and m143 knockout mutants attain organ titers equivalent to those attained by wild-type MCMV in Pkr knockout mice, suggesting that these viral proteins do not encode additional PKR-independent functions relevant for pathogenesis in vivo.
authors.affiliation: Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors.forename: Eleonore
authors.initials: E
authors.lastname: Ostermann
authors.affiliation: Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Warnecke
authors.affiliation: Junior Research Group Novel Vaccination Strategies and Early Immune Responses, Paul-Ehrlich-Institut, Langen, Germany.
authors.forename: Zoe
authors.initials: Z
authors.lastname: Waibler
authors.affiliation: Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany wolfram.brune@hpi.uni-hamburg.de.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Brune
mesh_terms: D000818:Animals; D018345:Mice, Knockout; D018146:Muromegalovirus / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000172:deficiency*
chemical_list: D014764:Viral Proteins; D019892:eIF-2 Kinase; C000605978:protein kinase R, mouse