title: Menadione-Induced Oxidative Stress Re-Shapes the Oxylipin Profile of Aspergillus flavus and Its Lifestyle.
abstract: Aspergillus flavus is an efficient producer of mycotoxins, particularly aflatoxin B₁, probably the most hepatocarcinogenic naturally-occurring compound. Although the inducing agents of toxin synthesis are not unanimously identified, there is evidence that oxidative stress is one of the main actors in play. In our study, we use menadione, a quinone extensively implemented in studies on ROS response in animal cells, for causing stress to A. flavus. For uncovering the molecular determinants that drive A. flavus in challenging oxidative stress conditions, we have evaluated a wide spectrum of several different parameters, ranging from metabolic (ROS and oxylipin profile) to transcriptional analysis (RNA-seq). There emerges a scenario in which A. flavus activates several metabolic processes under oxidative stress conditions for limiting the ROS-associated detrimental effects, as well as for triggering adaptive and escape strategies.
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. marco.zaccaria@uniroma1.it.
authors.forename: Marco
authors.initials: M
authors.lastname: Zaccaria
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. matteo.ludovici@uniroma1.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ludovici
authors.affiliation: Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Giovanni Amendola 165/A, Bari 70126, Italy. simonamarianna.sanzani@uniba.it.
authors.forename: Simona Marianna
authors.initials: SM
authors.lastname: Sanzani
authors.affiliation: Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Giovanni Amendola 165/A, Bari 70126, Italy. antonio.ippolito@uniba.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ippolito
authors.affiliation: Sequentia Biotech SL, Calle Comte d'Urgell 240 3°D, Barcelona 08036, Spain. raiesecigliano@sequentiabiotech.com.
authors.forename: Riccardo Aiese
authors.initials: RA
authors.lastname: Cigliano
authors.affiliation: Sequentia Biotech SL, Calle Comte d'Urgell 240 3°D, Barcelona 08036, Spain. wsanseverino@sequentiabiotech.com.
authors.forename: Walter
authors.initials: W
authors.lastname: Sanseverino
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. marzia.scarpari@uniroma1.it.
authors.forename: Marzia
authors.initials: M
authors.lastname: Scarpari
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. valeria.scala@uniroma1.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Scala
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. corrado.fanelli@uniroma1.it.
authors.forename: Corrado
authors.initials: C
authors.lastname: Fanelli
authors.affiliation: Department of Environmental Biology, University of Rome Sapienza, Largo Cristina di Svezia 24, Rome 00165, Italy. massimo.reverberi@uniroma1.it.
authors.forename: Massimo
authors.initials: M
authors.lastname: Reverberi
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D001231:Aspergillus flavus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D054883:Oxylipins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome / Q000187:drug effects; D024483:Vitamin K 3 / Q000494:pharmacology*
chemical_list: D000348:Aflatoxins; D054883:Oxylipins; D017382:Reactive Oxygen Species; D024483:Vitamin K 3
keywords: LC-MS/MS; RNA-seq; menadione; oxidative stress; oxylipins