title: Highly sensitive detection of bovine serum albumin based on the aggregation of triangular silver nanoplates.
abstract: A simple, fast and highly sensitive spectrophotometric method for the determination of bovine serum albumin (BSA) has been developed based on the interactions between triangular silver nanoplates (TAgNPs) and BSA in the presence of Britton-Robison buffer solution (BR). Particularly, the wavelength of absorption maximum (λ(max)) of TAgNPs is red shifted in the presence of BSA together with Britton-Robinson buffer solution (BR, pH=2.56), and the color of the solution changed from blue to light blue. This may be due to the interactions between BSA molecules on the surface of TAgNPs through electrostatic forces, hydrogen bonds, hydrophobic effects and van der Waals forces at pH2.56, which leads to the aggregation of TAgNPs. The determination of BSA was achieved by measuring the change of λ(max) corresponding to localized surface plasmon resonance (LSPR) from UV-visible spectrophotometry. It was found that the shift value in the wavelength of absorption maximum (Δλ, the difference in absorption maxima of the TAgNPs/BSA/BR mixture and the TAgNPs/BR mixture) was proportionate to the concentration of BSA in the range of 1.0 ng mL(-1) to 100.0 ng mL(-1) with the correlation coefficient of r=0.9969. The detection limit (3 σ/k) for BSA was found to be as low as 0.5 ng mL(-1).
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China; Hunan Xinshao Teachers Training College, China.
authors.forename: Ling Ling
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China.
authors.forename: Fang Fang
authors.initials: FF
authors.lastname: Ma
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China.
authors.forename: Yang Fang
authors.initials: YF
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China. Electronic address: l_yunfei927@163.com.
authors.forename: Yun Fei
authors.initials: YF
authors.lastname: Long
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education of China, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, 411201, China. Electronic address: qgxiao@hnust.edu.cn.
authors.forename: Qiu Guo
authors.initials: QG
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002417:Cattle; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012834:Silver / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D012710:Serum Albumin, Bovine; D012834:Silver
keywords: Bovine serum albumin; Britton–Robison buffer solution; Spectrophotometric detection; Triangular silver nanoplates