title: Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation.
abstract: Crk and CrkL are noncatalytic adaptor proteins necessary for the formation of neuromuscular synapses which function downstream of muscle-specific kinase (MuSK), a receptor tyrosine kinase expressed in skeletal muscle, and the MuSK binding protein Dok-7. How Crk/CrkL regulate neuromuscular endplate formation is not known. To better understand the roles of Crk/CrkL, we identified CrkL binding proteins using mass spectrometry and have identified Sorbs1 and Sorbs2 as two functionally redundant proteins that associate with the initiating MuSK/Dok-7/Crk/CrkL complex, regulate acetylcholine receptor (AChR) clustering in vitro, and are localized at synapses in vivo.
authors.affiliation: Novartis Institutes for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: Peter T
authors.initials: PT
authors.lastname: Hallock
authors.affiliation: Novartis Institutes for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: Sherry
authors.initials: S
authors.lastname: Chin
authors.affiliation: Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Blais
authors.affiliation: Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, New York, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Neubert
authors.affiliation: Novartis Institutes for Biomedical Research, Cambridge, Massachusetts, USA david.glass@novartis.com.
authors.forename: David J
authors.initials: DJ
authors.lastname: Glass
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009154:Mutation; D032446:Myoblasts / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D060066:Protein Interaction Maps; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D011950:Receptors, Cholinergic / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C082869:CRKL protein; D008840:Microfilament Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D011950:Receptors, Cholinergic; C000607590:Sorbs2 protein, mouse; C119201:ponsin