title: Capillary electrochromatography and nano-liquid chromatography coupled to nano-electrospray ionization interface for the separation and identification of estrogenic compounds.
abstract: Nano-LC and CEC were coupled to MS through a nanospray or a pressurized liquid-junction interface for the simultaneous separation and determination of 11 estrogenic compounds. Different stationary phases, that is, phenyl, C18, and C18 bidentate silica hydrate, were studied. For both techniques, the phenyl stationary phase was the best option, considering separation efficiency, selectivity, and resolution. Under the optimized conditions, the baseline separation of the target compounds (including estradiol and zearalanol epimers) was achieved in less than 20 min in nano-LC-MS and less than 13 min in CEC-MS. Molecular imprinted polymer SPE was used for extracting the target compounds from mineral water samples with the analysis of nano-LC-MS. The whole molecular imprinted polymer SPE nano-LC-MS method was validated through a recovery study at two levels of concentration. Sensitivity was improved by on-column focusing technique obtaining LODs in the range 1.4-55.4 ng/L.
authors.affiliation: Instituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche (C.N.R.), Monterotondo, Roma, Italia.|Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), La Laguna, Tenerife, España.
authors.forename: Giovanni
authors.initials: G
authors.lastname: D'Orazio
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), La Laguna, Tenerife, España.
authors.forename: Javier
authors.initials: J
authors.lastname: Hernández-Borges
authors.affiliation: Instituto Volcanológico de Canarias (INVOLCAN), Parque Taoro, Puerto de la Cruz, Tenerife, España.
authors.forename: María
authors.initials: M
authors.lastname: Asensio-Ramos
authors.affiliation: Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), La Laguna, Tenerife, España.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Rodríguez-Delgado
authors.affiliation: Instituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche (C.N.R.), Monterotondo, Roma, Italia.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Fanali
mesh_terms: D053801:Capillary Electrochromatography / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004967:Estrogens / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D036103:Nanotechnology / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004967:Estrogens; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Electrochromatography; Estrogens; MS; Molecular imprinted polymer; Nano-LC