title: [Arginase Level in Suspended Red Blood Cells Storaged for Different Time].
abstract: OBJECTIVE
  To explore the effect of storage time on arginase level, and the possible source of arginase in suspended red blood cells (RBC).
  
  
  METHODS
  The arginase and myeloperoxidase (MPO) levels in suspended RBC and control plasma were detected by ELISA. The free hemoglobin level in suspended RBC and control plasma were detected by colorimetric method. The relationship between arginase level, MPO level and free hemoglobin level in suspended RBC was analyzed by the related methods.
  
  
  RESULTS
  The arginase and free hemoglobin levels in suspended RBC were higher than those in control plasma. Otherwise, MPO level was not significantly different between suspended RBC and control plasma. All of them did not increase along with prolonging of storage time. There was not a significant correlation between arginase level and free hemoglobin level in suspended RBC of different storage time (r = 0.03), but arginase level positively correlated with MPO level in the suspended RBC of different storage time (r = 0.76).
  
  
  CONCLUSIONS
  The arginase level in suspended RBC storaged for different time increases significantly, but not along with prolonging of storage time. The main possible source of arginase in the suspended RBC is the residual white blood cell, especially neutrophils.
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Fan
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China.
authors.forename: Hao-Bo
authors.initials: HB
authors.lastname: Huang
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China.
authors.forename: Shi-Jin
authors.initials: SJ
authors.lastname: Wei
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Department of Hematology, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China. E-mail: fudanhui@189.cn.
authors.forename: Dan-Hui
authors.initials: DH
authors.lastname: Fu
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zeng
authors.affiliation: Department of Blood Transfusion, The Union Hospital of Fujian Medical University, Fujian Institute of Hematology, Fuzhou 350001, Fujian Province, China.|Fujian Provincial Quality Control Center for Clinical Blood Transfusion, Fuzhou 350001, Fujian Province, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Huang
authors.affiliation: Department of Anesthesia, The Second Affiliated Hospital, Fujian Medical University, Quanzhou 362000, Fujian Province, China.
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Hong
mesh_terms: D001119:Arginase / Q000737:chemistry*; D001793:Blood Preservation; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D009195:Peroxidase / Q000737:chemistry; D010949:Plasma / Q000201:enzymology; D013997:Time Factors
chemical_list: D009195:Peroxidase; D001119:Arginase