title: Longitudinal orientation of cross-linked polypeptide γ chains in fibrin fibrils.
abstract: The crosslinking of fibrin γ-polypeptide chains under the influence of the plasma fibrin-stabilizing factor (FXIIIa), which causes their conversion to γ-γ dimers, is the major enzyme reaction of covalent fibrin stabilization. We studied the self-assembly of soluble cross-linked fibrin oligomers. The results of analytical ultracentrifugation as well as elastic and dynamic light scattering showed that the double-stranded fibrin oligomers formed under the influence of moderate concentrations of urea are cross-linked only due to formation of γ-γ dimers, which can dissociate into single-stranded structure when the concentration of urea increases. This fact proves that γ-γ dimers are formed in the end-to-end manner.
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia. markrosenfeld@rambler.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Rosenfeld
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: V B
authors.initials: VB
authors.lastname: Leonova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Bychkova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kostanova
authors.affiliation: Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia.
authors.forename: M I
authors.initials: MI
authors.lastname: Biryukova
mesh_terms: D019281:Dimerization; D000067493:Dynamic Light Scattering; D026122:Factor XIIIa / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D055503:Protein Multimerization; D014461:Ultracentrifugation; D014508:Urea / Q000737:chemistry
chemical_list: D014508:Urea; D005337:Fibrin; D026122:Factor XIIIa