title: The murine cytomegalovirus immunoevasin gp40 binds MHC class I molecules to retain them in the early secretory pathway.
abstract: In the presence of the murine cytomegalovirus (mCMV) gp40 (m152) protein, murine major histocompatibility complex (MHC) class I molecules do not reach the cell surface but are retained in an early compartment of the secretory pathway. We find that gp40 does not impair the folding or high-affinity peptide binding of the class I molecules but binds to them, leading to their retention in the endoplasmic reticulum (ER), the ER-Golgi intermediate compartment (ERGIC) and the cis-Golgi, most likely by retrieval from the cis-Golgi to the ER. We identify a sequence in gp40 that is required for both its own retention in the early secretory pathway and for that of class I molecules.
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Linda
authors.initials: L
authors.lastname: Janßen
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Venkat Raman
authors.initials: VR
authors.lastname: Ramnarayan
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Aboelmagd
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Maro
authors.initials: M
authors.lastname: Iliopoulou
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Hein
authors.affiliation: Institute of Biology, Center for Structural and Cell Biology in Medicine, University of Lübeck, 23562 Lübeck, Germany.
authors.forename: Irina
authors.initials: I
authors.lastname: Majoul
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Fritzsche
authors.affiliation: Institute of Virology, University Medical Center, University of Freiburg, 79104 Freiburg, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Halenius
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany s.springer@jacobs-university.de.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Springer
mesh_terms: D000818:Animals; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D018146:Muromegalovirus / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D055571:Secretory Pathway; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D015395:Histocompatibility Antigens Class I; D010455:Peptides; D014764:Viral Proteins
keywords: Antigen presentation; ERGIC and cis-Golgi retention; Early secretory pathway; Immune evasion; Murine cytomegalovirus