title: The increase of circulating PD-L1-expressing CD68(+) macrophage in ovarian cancer.
abstract: Tumor-associated macrophages (TAMs) have been characterized as a critical population of immunosuppressive cells in a variety of tumor types. PD-L1 (also termed B7-H1) has been described to exert co-inhibitory and immune regulatory functions. Here, in ovarian cancer, PD-L1 is selectively overexpressed on some TAM compared that of benign ovarian disease. When expanding the data in peripheral blood, the proportion of PD-L1(+)CD68(+) cell among CD68(+) cells and the intensity of PD-L1 staining on CD68(+) cell in healthy group were similar to that observed in ovarian cyst group; instead, these two measures were significantly higher in ovarian cancer group, thereafter related to TNM stage. Interestingly, intracellular levels of IL-10, IL-6, TNF-α, and IFN-γ in PD-L1(+)CD68(+) macrophage were higher than those in PD-L1(-)CD68(+) macrophage, especially IL-6 expression. Based on the PD-L1 receptor PD-1 expression on tumor-infiltrating cytotoxic cells, our data supported that expression of PD-L1 on TAM promoted apoptosis of T cells via interaction with PD-1 on CD8(+)T cells. Taken together, these results suggested that PD-L1-expressing macrophage represents a novel suppressor cell population in ovarian cancer, which contributes immune escape of ovarian cancer.
authors.affiliation: Clinical Immunology Institute, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Qu
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Clinical Immunology Institute, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Biology and Basic Medical Sciences, Biotechnology Research Institute, Soochow University, 199 Renai Road, Suzhou, 215123, China. xlyb99@163.com.
authors.forename: Yi-Bei
authors.initials: YB
authors.lastname: Zhu
authors.affiliation: Clinical Immunology Institute, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, People's Republic of China. xueguangzh@126.com.
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D060890:B7-H1 Antigen / Q000096:biosynthesis* / Q000235:genetics; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D061026:Programmed Cell Death 1 Receptor / Q000096:biosynthesis* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D060890:B7-H1 Antigen; D007371:Interferon-gamma; D016753:Interleukin-10; D015850:Interleukin-6; D061026:Programmed Cell Death 1 Receptor; D014409:Tumor Necrosis Factor-alpha; C423236:CD274 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C508609:IL10 protein, human; C508600:IL6 protein, human; C105992:PDCD1 protein, human
keywords: Immune escape; Ovarian carcinoma; PD-L1; Tumor-associated macrophages