title: Eryptosis Indices as a Novel Predictive Parameter for Biocompatibility of Fe3O4 Magnetic Nanoparticles on Erythrocytes.
abstract: Fe3O4 magnetic nanoparticles (Fe3O4-MNPs) have been widely used in clinical diagnosis. Hemocompatibility of the nanoparticles is usually evaluated by hemolysis. However, hemolysis assessment does not measure the dysfunctional erythrocytes with pathological changes on the unbroken cellular membrane. The aim of this study is to evaluate the use of suicidal death of erythrocytes (i.e. eryptosis indices) as a novel predictive and prognostic parameter, and to determine the impact of Fe3O4-MNPs on cellular membrane structure and the rheology properties of blood in circulation. Our results showed that phosphatidylserine externalization assessment was significantly more sensitive than classical hemolysis testing in evaluating hemocompatibility. Although no remarkable changes of histopathology, hematology and serum biochemistry indices were observed in vivo, Fe3O4-MNPs significantly affected hemorheology indices including erythrocyte deformation index, erythrocyte rigidity index, red blood cell aggregation index, and erythrocyte electrophoresis time, which are related to the mechanical properties of the erythrocytes. Oxidative stress induced calcium influx played a critical role in the eryptotic activity of Fe3O4-MNPs. This study demonstrated that Fe3O4-MNPs cause eryptosis and changes in flow properties of blood, suggesting that phosphatidylserine externalization can serve as a predictive parameter for hemocompatibility assay.
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ran
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, The Second Affiliated Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000008:administration & dosage*; D002449:Cell Aggregation / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000008:administration & dosage*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D010718:Phosphatidylserines / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D058185:Magnetite Nanoparticles; D010718:Phosphatidylserines; D052203:Ferrosoferric Oxide