title: A Method for Cell Culture and Maintenance of Ammonia-Oxidizing Archaea in Agar Stab.
abstract: Ammonia oxidizing archaea (AOA) are predominantly found and closely linked with geochemical cycling of nitrogen in non-extreme habitats. However, these strains have mainly been investigated using liquid cultures of enriched cells. Here, we provide an agar stab as a simple and reliable means of cultivating and maintaining AOA.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Yong-Bong Dong, Buk-Gu, Gwangju, 500-757 Korea.
authors.forename: Yeon-Jin
authors.initials: YJ
authors.lastname: Chu
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Yong-Bong Dong, Buk-Gu, Gwangju, 500-757 Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Yong-Bong Dong, Buk-Gu, Gwangju, 500-757 Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Shin
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Yong-Bong Dong, Buk-Gu, Gwangju, 500-757 Korea.
authors.forename: Geun-Joong
authors.initials: GJ
authors.lastname: Kim
keywords: Agar stab; Ammonia-oxidizing archaea; Cultivation; Immunofluorescence staining