title: Cytotoxicity of a New Nano Zinc-Oxide Eugenol Sealer on Murine Fibroblasts.
abstract: BACKGROUND
  The aim of this study was to evaluate the cytotoxicity of a new nano zinc-oxide eugenol (NZOE) sealer in comparison with AH-26 and Pulpdent root canal sealers.
  
  
  METHODS
  The L929 mouse fibroblast cells were cultivated and incubated for 24, 48 or 72 h with different dilutions (1/1, 1/2, 1/4, 1/8, 1/16 and 1/32) of culture media previously exposed to either of the test sealers naming NZOE, AH-26 or Pulpdent. At the end of incubation period, the effect of sealers on cell viability was evaluated using Mosmann's Tetrazolium Toxicity (MTT) colorimetric assay. The data was compared using the one-way analysis of variance (ANOVA) followed by the Tukey's post hoc test for multiple comparisons.
  
  
  RESULTS
  After 24, 48 or 72 h, both NZOE and Pulpdent sealers inhibited cell viability at 1/1, 1/2 and 1/8 dilutions. Within the 24 and 48 h, the AH-26 sealer reduced the cell viability at all dilutions except the 1/32 solution; however after 72 h even the 1/32 dilution was cytotoxic.
  
  
  CONCLUSIONS
  The biocompatibility of the nano zinc-oxide eugenol sealer was comparable to Pulpdent sealer and lower than AH-26.
authors.affiliation: Dental Material Research Center, Department of Endodontics, Dental School, Mashhad University of Medical Sciences, Mashhad, Iran;
authors.forename: Maryam
authors.initials: M
authors.lastname: Javidi
authors.affiliation: Dental Material Research Center, Department of Endodontics, Dental School, Mashhad University of Medical Sciences, Mashhad, Iran;
authors.forename: Mina
authors.initials: M
authors.lastname: Zarei
authors.affiliation: Department of Endodontics, Dental School, Mazandaran University of Medical Sciences, Sari, Iran ;
authors.forename: Salma
authors.initials: S
authors.lastname: Omidi
authors.affiliation: Pharmacological Research Center of Medicinal Plants, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran ;
authors.forename: Ahmad
authors.initials: A
authors.lastname: Ghorbani
authors.affiliation: Dental Material Research Center, Department of Endodontics, Dental School, Mashhad University of Medical Sciences, Mashhad, Iran;
authors.forename: Maryam
authors.initials: M
authors.lastname: Gharechahi
authors.affiliation: Student Research Committee (SRC), Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Shayani Rad
keywords: Cytotoxicity; MTT Assay; Nanoparticles; Root Canal Sealer; Zinc-Oxide Eugenol