title: Interference on cytoplasmic polyadenylation element-binding proteins affects the invasion ability of glioma stem cells.
abstract: Glioma stem cells derived from primary cultures were divided into an experiment group, a control group, and a blank group and subjected to cytoplasmic polyadenilation element-binding protein (CPEBs) interference, transfection with empty vector, and normal culture, respectively, to compare their invasion abilities. Western blotting showed that siRNA-3 had the strongest interfering effect on CPEBs. CPEBs were expressed in the experiment group with green fluorescence at an expression rate of over 70%. Significantly lower CPEB expression was observed in the experiment group compared to in the control and blank groups (P < 0.05). After 48-h treatment, the apoptotic rate in the experiment group was 21.43%, which was significantly higher than that in the blank (0.51%) and control (1.43%) groups (P < 0.05). After 3 days of treatment, the experiment group grew significantly more slowly than did the control and blank groups (P < 0.05). The transwell invasion assay showed that significantly fewer cells in the experiment group penetrated the membrane than did cells in the control and blank groups (P < 0.05). After CPEB interference, the growth, proliferation, and invasion of glioma stem cells were substantially inhibited, providing support for targeted therapy of glioma and for improving prognosis.
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University, Kaifeng, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University, Kaifeng, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Huo
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University, Kaifeng, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Huaihe Hospital, Henan University, Kaifeng, China chenxbdn@163.com.
authors.forename: X B
authors.initials: XB
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology*; D034741:RNA, Small Interfering; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C490386:CPEB1 protein, human; D034741:RNA, Small Interfering; D014157:Transcription Factors; D039221:mRNA Cleavage and Polyadenylation Factors