title: Holographic analysis on deformation and restoration of malaria-infected red blood cells by antimalarial drug.
abstract: Malaria parasites induce morphological, biochemical, and mechanical changes in red blood cells (RBCs). Mechanical variations are closely related to the deformability of individual RBCs. The deformation of various RBCs, including healthy and malaria-infected RBCs (iRBCs), can be directly observed through quantitative phase imaging (QPI). The effects of chloroquine treatment on the mechanical property variation of iRBCs were investigated using time-resolved holographic QPI of single live cells on a millisecond time scale. The deformabilities of healthy RBCs, iRBCs, and drug-treated iRBCs were compared, and the effect of chloroquine on iRBC restoration was experimentally examined. The present results are beneficial to elucidate the dynamic characteristics of iRBCs and the effect of the antimalarial drug on iRBCs.
authors.affiliation: Pohang University of Science and Technology, Department of Mechanical Engineering, San 31. Hyoja-dong, Namgu, Pohang 790-784, Republic of Korea.
authors.forename: Hyeokjun
authors.initials: H
authors.lastname: Byeon
authors.affiliation: Pohang University of Science and Technology, Division of Integrative Biosciences and Biotech, San 31. Hyoja-dong, Namgu, Pohang 790-784, Republic of Korea.
authors.forename: Young-Ran
authors.initials: YR
authors.lastname: Ha
authors.affiliation: Pohang University of Science and Technology, Department of Mechanical Engineering, San 31. Hyoja-dong, Namgu, Pohang 790-784, Republic of KoreabPohang University of Science and Technology, Division of Integrative Biosciences and Biotech, San 31. Hyoja-don.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000962:Antimalarials / Q000008:administration & dosage; D048429:Cell Size / Q000187:drug effects; D058948:Cell Tracking / Q000295:instrumentation; D002478:Cells, Cultured; D002738:Chloroquine / Q000008:administration & dosage*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology* / Q000821:virology*; D006696:Holography / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D010963:Plasmodium falciparum / Q000187:drug effects / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016896:Treatment Outcome
chemical_list: D000962:Antimalarials; D002738:Chloroquine