title: Optical Control of Peroxisomal Trafficking.
abstract: The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine , Aurora, Colorado 80045, United States.
authors.forename: Jessica I
authors.initials: JI
authors.lastname: Spiltoir
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago , Chicago, Illinois 60637, United States.
authors.forename: Devin
authors.initials: D
authors.lastname: Strickland
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago , Chicago, Illinois 60637, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Glotzer
authors.affiliation: Department of Pharmacology, University of Colorado School of Medicine , Aurora, Colorado 80045, United States.
authors.forename: Chandra L
authors.initials: CL
authors.lastname: Tucker
mesh_terms: D000818:Animals; D018554:Avena / Q000737:chemistry*; D019556:COS Cells; D002522:Chlorocebus aethiops; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008027:Light; D057054:Molecular Imaging / Q000379:methods; D020675:Peroxisomes / Q000378:metabolism*; D056949:Phototropins / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D015202:Protein Engineering / Q000379:methods; D021381:Protein Transport; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D056949:Phototropins; D010940:Plant Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins
keywords: LOV domain; caged peptide; optogenetics; peroxisome; trafficking