title: Circulating microRNAs correlated with the level of coronary artery calcification in symptomatic patients.
abstract: The purpose of this study was to find the circulating microRNAs (miRNAs) co-related with the severity of coronary artery calcification (CAC), and testify whether the selected miRNAs could reflect the obstructive coronary artery disease in symptomatic patients. Patients with chest pain and moderated risk for coronary artery disease (CAD) were characterized with coronary artery calcium score (CACS) from cardiac computed tomography (CT). We analyzed plasma miRNA levels of clinical matched 11 CAC (CACS > 100) and 6 non-CAC (CACS = 0) subjects by microarray profile. Microarray analysis identified 34 differentially expressed miRNAs between CAC and non CAC groups. Eight miRNAs (miR-223, miR-3135b, miR-133a-3p, miR-2861, miR-134, miR-191-3p, miR-3679-5p, miR-1229 in CAC patients) were significantly increased in CAC plasma in an independent clinical matched cohort. Four miRNAs (miR-2861, 134, 1229 and 3135b) were correlated with the degree of CAC. Validation test in angiographic cohort showed that miR-134, miR-3135b and miR-2861 were significantly changed in patients with obstructive CAD . We identified three significantly upregulated circulating miRNAs (miR-134, miR-3135b and 2861) correlated with CAC while detected obstructive coronary disease in symptomatic patients.
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Shukuan
authors.initials: S
authors.lastname: Ling
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Dingsheng
authors.initials: D
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Song
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Chai
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Qinyi
authors.initials: Q
authors.lastname: Dai
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Radiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Zhanming
authors.initials: Z
authors.lastname: Fan
authors.affiliation: Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing, China.
authors.forename: Yu Jie
authors.initials: YJ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing, China.
authors.forename: Yingxian
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D002114:Calcinosis / Q000473:pathology*; D003324:Coronary Artery Disease / Q000097:blood* / Q000473:pathology*; D003331:Coronary Vessels / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D046228:Microarray Analysis; D008875:Middle Aged; D015854:Up-Regulation / Q000502:physiology
chemical_list: D035683:MicroRNAs