title: Serum metabolomics in rats models of ketamine abuse by gas chromatography-mass spectrometry.
abstract: This study aims to evaluate the effect of ketamine on rats by examining the differences in serum metabolites between ketamine abuse group (Ket-group) and control group (Con-group). Compared to the Con-group, the level of phosphate, propanoic acid, ribitol and d-fructose of the Ket-group increased after continuous intraperitoneal administration of ketamine for 7 days, while the level of alanine, glycine, butanoic acid, valine, l-serine, l-proline, mannonic acid, octadecanoic acid and cholesterol decreased. After 14 days' administration, the level of alanine, butanoic acid, valine, l-leucine, phosphate, l-serine, l-threonine, propanetricarboxylic acid, hexadecanoic acid and oleic acid of the ketamine group increased while the level of mannonic acid, octadecanoic acid and cholesterol decreased. After stopping ketamine administration for 2 days, the level of butanoic acid, phosphate, aminomalonic acid, gluconic acid, hexadecanoic acid, oleic acid and arachidonic acid of Ket-group increased, while the level of glycine, l-lysine and cholesterol decreased. This study can provide invaluable information for the metabolites changes due to ketamine abuse.
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Sun
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui 323000, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Kezhi
authors.initials: K
authors.lastname: Lin
authors.affiliation: Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: guanyanglinwzmc@gmail.com.
authors.forename: Guanyang
authors.initials: G
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address: hulufeng79@sina.com.
authors.forename: Lufeng
authors.initials: L
authors.lastname: Hu
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007649:Ketamine / Q000097:blood* / Q000378:metabolism* / Q000494:pharmacology; D016018:Least-Squares Analysis; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D007649:Ketamine
keywords: Abuse; GC/MS; Ketamine; Metabolomics; Serum