title: Histological and proteomic profile of diabetic versus non-diabetic dilated cardiomyopathy.
abstract: BACKGROUND
  Diabetic cardiomyopathy (DbCM) is indistinguishable from idiopathic dilated cardiomyopathy (IDCM) as specific histological and/or biochemical markers are unavailable.
  
  
  RESULTS
  Comparative histology, electron microscopy, morphometry for cell volume composition and myocardial fibrosis, reactive oxygen species (ROS), polymerase chain reaction for cardiotropic viruses, immunohistochemistry for nitrotyrosine, inducible nitric oxide synthase (iNOS), 8-hydroxydeoxyguanosine (8-OH-dG) and proteomics have been evaluated in endomyocardial biopsies from 9 patients (pts) (5 male and 4 female, mean age 61 ± 13 years) with DbCM (left ventricular end-diastolic diameter 65 ± 2.3mm; ejection fraction 27 ± 6) and type 2 diabetes mellitus and 9 pts with IDCM (mean age 60 ± 9 years) matched for sex, age and severity of left ventricular (LV) dysfunction. Controls were surgical biopsies from 9 pts with mitral stenosis and normal LV dimensions and function. No qualitative morphological changes were observed between DbCM and IDCM although mitochondrial damage and myofibrillolysis appeared more pronounced in DbCM. ROS were 5 times higher in DbCM than in IDCM and controls and were associated with higher expression of cytoplasm iNOS and nitrotyrosine and nuclear 8-OH-dG. Apoptosis was 14 times higher in DbCM than in IDCM and 41 times higher than in controls. Proteomic profile showed in DbCM a reduced expression of proteins related to beta-oxidation and detoxification pathway.
  
  
  CONCLUSIONS
  DbCM is a distinctive ROS-mediated disorder with oxidative damage of myocyte's structural proteins and DNA causing cell dysfunction and death. Reduced expression of beta-oxidation proteins suggests a decline of energy production and of mitochondrial function.
authors.affiliation: Department of Cardiovascular, Nefrologic, Anestesiologic and Geriatric Sciences, La Sapienza University, Italy; National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Frustaci
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Ciccosanti
authors.affiliation: Department of Cardiovascular, Nefrologic, Anestesiologic and Geriatric Sciences, La Sapienza University, Italy; National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Chimenti
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Nardacci
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy; Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Corazzari
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Romina
authors.initials: R
authors.lastname: Verardo
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ippolito
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Petrosillo
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy; Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Lecce 73100, Italy.
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Fimia
authors.affiliation: National Institute for Infectious Diseases IRCCS 'L. Spallanzani', Rome, Italy; Department of Biology, University of Rome 'Tor Vergata', Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Piacentini
mesh_terms: D000368:Aged; D001706:Biopsy; D002311:Cardiomyopathy, Dilated / Q000378:metabolism* / Q000473:pathology*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology*; D058065:Diabetic Cardiomyopathies / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism; D013318:Stroke Volume / Q000502:physiology
chemical_list: D017382:Reactive Oxygen Species
keywords: Cardiomyopathy; Diabetes; Oxidative damage; Reactive oxygen species