title: Conjugated bile acids in gallbladder bile and serum as potential biomarkers for cholesterol polyps and adenomatous polyps.
abstract: OBJECTIVE
  To identify conjugated bile acids in gallbladder bile and serum as possible biomarkers for cholesterol polyps (CPs) and adenomatous polyps (APs).
  
  
  METHODS
  Gallbladder bile samples and serum samples were collected from 18 patients with CPs (CP group), 9 patients with APs (AP group), and 20 patients with gallstones (control group) from March to November, 2013. High performance liquid chromatography (HPLC) assay with ultraviolent detection was used to detect the concentration of 8 conjugated bile acids (glycocholic acid, GCA; taurocholic acid, TCA; glycochenodeoxycholic acid, GCDCA; taurochenodeoxycholic acid, TCDCA; glycodeoxycholic acid, GDCA; taurodeoxycholic acid, TDCA; taurolithocholic acid, TLCA; tauroursodeoxycholic acid, TUDCA) in bile samples and serum samples. The diagnostic efficacy of serum GCA, GCDCA and TCDCA was evaluated.
  
  
  RESULTS
  These 8 conjugated bile acids in gallbladder bile and serum were completely identified within 10 minutes with good linearity (correlation coefficient: R&gt;0.9900; linearity range: 3.91-500 µg/mL). Among these conjugated bile acids, the levels of gallbladder bile GCDCA and TCDCA in the CP group were significantly higher than those in the AP group (p&lt;0.05). Furthermore, serum GCDCA and TCDCA as well as GCA were significantly higher in the AP group than the CP group (p&lt;0.05). Serum GCDCA alone (≤12 µg/mL) had relatively better diagnostic efficacy than the other conjugated bile acids.
  
  
  CONCLUSIONS
  The levels of serum GCA, GCDCA and TCDCA may be valuable for differentiation of APs and CPs.
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Mei-Fen
authors.initials: MF
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Ge
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Ma
authors.affiliation: Department of General Surgery, The First Hospital of China Medical University, Shenyang - China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Ye
mesh_terms: D018256:Adenomatous Polyps / Q000097:blood* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001826:Body Fluids / Q000378:metabolism; D002763:Cholecystectomy; D002784:Cholesterol / Q000097:blood*; D005260:Female; D005704:Gallbladder / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D001647:Bile Acids and Salts; D014408:Biomarkers, Tumor; D002784:Cholesterol