title: Chemotherapy promotes tumour cell hybridization in vivo.
abstract: Spontaneous cell-cell fusion has been recognized to be an important mechanism for tissue and organ development and repair. In cancer, cell fusion is critically involved in tumourigenesis, metastasis and drug resistance, as illustrated by in vitro experiments. However, there has been no direct detection of tumour cell fusion or hybridization in an in vivo tumour environment, and the features of hybridized cells under selective pressures, such as chemotherapy, are unknown. Here, we expressed two fluorescent marker proteins in the human breast cancer cell line SKBR3 to detect tumour cell hybridization in vivo and performed a xenograft chemotherapy experiment in mice to evaluate the chemotherapeutic response of the hybrids. The mice treated by epirubicin showed that chemotherapy promoted tumour cell hybridization in vivo, which elicited the production of more hybrids in the outer section of the tumour. These results provide the first in vivo evidence of tumour cell fusion and indicate that chemotherapy may contribute to a poor prognosis by enriching for fused cells, which are more malignant. It is therefore necessary to reassess chemotherapy strategies.
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, Sun Yat-sen University, #135 Xinggang West, Guangzhou, 510275, China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, Sun Yat-sen University, #135 Xinggang West, Guangzhou, 510275, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biocontrol, College of Ecology and Evolution, Sun Yat-sen University, #135 Xinggang West, Guangzhou, 510275, China. liul47@mail.sysu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D063646:Carcinogenesis / Q000187:drug effects; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015251:Epirubicin / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D011379:Prognosis; D023041:Xenograft Model Antitumor Assays
chemical_list: D015251:Epirubicin
keywords: Cell fusion; Chemotherapy resistance; In vivo; Poor prognosis