title: Interleukin-17A rs2275913, Interleukin-17F rs763780 and rs2397084 gene polymorphisms as possible risk factors in Juvenile lupus and lupus related nephritis.
abstract: There are no reports about the association of interleukin (IL)-17A and IL-17F gene polymorphism and susceptibility to pediatric systemic lupus erythematosus (pSLE).
  
  
  OBJECTIVE
  To examine the possible role of IL-17A rs2275913, IL-17F rs763780 and rs2397084 polymorphisms as risk factors for pSLE in a cohort of Egyptian children and to investigate their association with the clinico-pathological features including lupus nephritis (LN).
  
  
  METHODS
  Typing of IL-17A and IL-17F polymorphisms was done using restriction fragment length polymorphism for 115 children with SLE and 259 age- and sex-matched healthy controls.
  
  
  RESULTS
  No significant differences were found between pSLE patients and healthy controls for the allele and genotype frequencies of IL-17A rs2275913, IL-17F rs763780 and rs2397084 (p > 0.05). However, the combined genotype GGAGAA and the haplotype GGA had significant association with pSLE (pc = 0.042 and <0.001, respectively). The AA genotype of IL-17F rs763780 is more frequent in female patients (p = 0.002) and the AA genotype of IL-17F rs2397084 is more associated with positivity of ds-DNA (p = 0.007). No more associations were found for the demographic and clinical data of pSLE patients including risk of LN development, risk of non-remission, overall survival, activity and chronicity indices.
  
  
  CONCLUSIONS
  The GGAGAA combined genotype and the GGA haplotype of IL-17A rs2275913, IL-17F rs763780 and rs2397084 can be considered risk factors for the development of SLE in Egyptian children. IL-17A rs2275913, IL-17F rs763780 and rs2397084 are not related to the LN development, SLE disease activity or overall survival.
authors.affiliation: a Pediatric Nephrology Unit, Mansoura University Children's Hospital , Mansoura , Egypt .
authors.forename: Ayman
authors.initials: A
authors.lastname: Hammad
authors.affiliation: b Clinical Immunology Unit, Clinical Pathology Department & Mansoura Research Center for Cord Stem Cells (MARC_CSC), Faculty of Medicine, Mansoura University , Mansoura , Egypt .
authors.forename: Youssef M
authors.initials: YM
authors.lastname: Mosaad
authors.affiliation: c Rheumatology and Rehabilitation Department, Mansoura University Hospital , Mansoura , Egypt .
authors.forename: Enas M
authors.initials: EM
authors.lastname: Hammad
authors.affiliation: d Dermatology and Andrology Department, Mansoura Faculty of Medicine , Mansoura , Egypt .
authors.forename: Samir
authors.initials: S
authors.lastname: Elhanbly
authors.affiliation: c Rheumatology and Rehabilitation Department, Mansoura University Hospital , Mansoura , Egypt .
authors.forename: Sherif R
authors.initials: SR
authors.lastname: El-Bassiony
authors.affiliation: b Clinical Immunology Unit, Clinical Pathology Department & Mansoura Research Center for Cord Stem Cells (MARC_CSC), Faculty of Medicine, Mansoura University , Mansoura , Egypt .
authors.forename: Mohammad F
authors.initials: MF
authors.lastname: Al-Harrass
authors.affiliation: a Pediatric Nephrology Unit, Mansoura University Children's Hospital , Mansoura , Egypt .
authors.forename: Riham
authors.initials: R
authors.lastname: Eid
authors.affiliation: e Clinical Hematology Unit, Clinical Pathology Department, Faculty of Medicine, Mansoura University , Mansoura , Egypt .
authors.forename: Omar A
authors.initials: OA
authors.lastname: Sharaf Eldein
authors.affiliation: f Pediatric Cardiology Unit and.
authors.forename: Gehan Attia
authors.initials: GA
authors.lastname: Alsawah
authors.affiliation: g Genetics Unit, Mansoura University Children's Hospital , Mansoura , Egypt , and.
authors.forename: Sohier
authors.initials: S
authors.lastname: Yahia
authors.affiliation: h Laboratory Medicine Department , Mansoura Fever Hospital, Ministry of Health , Mansoura , Egypt.
authors.forename: Iman M
authors.initials: IM
authors.lastname: Fawzy
mesh_terms: D000293:Adolescent; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D004534:Egypt; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology / Q000401:mortality / Q000473:pathology; D008181:Lupus Nephritis / Q000235:genetics* / Q000276:immunology / Q000401:mortality / Q000473:pathology; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D016019:Survival Analysis
chemical_list: C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17
keywords: Egyptian; IL-17; Juvenile; SLE; SNPs; nephritis