title: VPAC1 Targeted (64)Cu-TP3805 Positron Emission Tomography Imaging of Prostate Cancer: Preliminary Evaluation in Man.
abstract: OBJECTIVE
  To evaluate (64)Cu-TP3805 as a novel biomolecule, to positron emission tomography (PET) image prostate cancer (PC), at the onset of which VPAC1, the superfamily of G protein-coupled receptors, is expressed in high density on PC cells, but not on normal cells.
  
  
  METHODS
  Twenty-five patients undergoing radical prostatectomy were PET/X-ray computerized tomography imaged preoperatively with (64)Cu-TP3805. Standardized maximum uptake (SUVmax) values were determined and malignant lesions (standardized uptake value > 1.0) counted, and compared with histologic findings. Whole-mount pathology slides from 6 VPAC1 PET imaged patients, 3 benign prostatic hyperplasia patients, 1 malignant and 1 benign lymph node underwent digital autoradiography (DAR) after (64)Cu-TP3805 incubation and were compared to hematoxylin- and eosin-stained slides.
  
  
  RESULTS
  In 25 patients who underwent PET imaging, 212 prostate gland lesions had SUVmax > 1.0 vs 127 lesions identified by histology of biopsy tissues. The status of the additional 85 PET identified prostate lesions remains to be determined. In 68 histologic slides from 6 PET imaged patients, DAR identified 105 of 107 PC foci, 19 of 19 high-grade prostatic intraepithelial neoplasias, and ejaculatory ducts and verumontanum involved with cancer. Additionally, DAR found 9 PC lesions not previously identified histologically. The positive and negative lymph nodes were correctly identified, and in 3 of 3 benign prostatic hyperplasia patients and 5 of 5 cysts, DAR was negative.
  
  
  CONCLUSIONS
  This feasibility study demonstrated that (64)Cu-TP3805 delineates PC in vivo and ex vivo, provided normal images for benign masses, and is worthy of further studies.
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Sushil
authors.initials: S
authors.lastname: Tripathi
authors.affiliation: Department of Urology, Thomas Jefferson University, Philadelphia, PA; Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Edouard J
authors.initials: EJ
authors.lastname: Trabulsi
authors.affiliation: Department of Urology, Thomas Jefferson University, Philadelphia, PA; Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Leonard
authors.initials: L
authors.lastname: Gomella
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Sung
authors.initials: S
authors.lastname: Kim
authors.affiliation: Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA; Department of Pathology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Peter
authors.initials: P
authors.lastname: McCue
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Charles
authors.initials: C
authors.lastname: Intenzo
authors.affiliation: Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA; Department of Pathology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Ruth
authors.initials: R
authors.lastname: Birbe
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Ashish
authors.initials: A
authors.lastname: Gandhe
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA.
authors.forename: Pardeep
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Department of Radiology, Thomas Jefferson University, Philadelphia, PA; Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA. Electronic address: madhukar.thakur@jefferson.edu.
authors.forename: Mathew
authors.initials: M
authors.lastname: Thakur
mesh_terms: D000328:Adult; D000368:Aged; D056831:Coordination Complexes; D005240:Feasibility Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides; D049268:Positron-Emission Tomography; D011470:Prostatic Hyperplasia; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000096:biosynthesis*
chemical_list: C000609414:64Cu-TP3805; D056831:Coordination Complexes; D010455:Peptides; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I