title: Biomolecular templating of functional hybrid nanostructures using repeat protein scaffolds.
abstract: The precise synthesis of materials and devices with tailored complex structures and properties is a requisite for the development of the next generation of products based on nanotechnology. Nowadays, the technology for the generation of this type of devices lacks the precision to determine their properties and is accomplished mostly by 'trial and error' experimental approaches. The use of bottom-up approaches that rely on highly specific biomolecular interactions of small and simple components is an attractive approach for the templating of nanoscale elements. In nature, protein assemblies define complex structures and functions. Engineering novel bio-inspired assemblies by exploiting the same rules and interactions that encode the natural diversity is an emerging field that opens the door to create nanostructures with numerous potential applications in synthetic biology and nanotechnology. Self-assembly of biological molecules into defined functional structures has a tremendous potential in nano-patterning and the design of novel materials and functional devices. Molecular self-assembly is a process by which complex 3D structures with specified functions are constructed from simple molecular building blocks. Here we discuss the basis of biomolecular templating, the great potential of repeat proteins as building blocks for biomolecular templating and nano-patterning. In particular, we focus on the designed consensus tetratricopeptide repeats (CTPRs), the control on the assembly of these proteins into higher order structures and their potential as building blocks in order to generate functional nanostructures and materials.
authors.affiliation: IMDEA-Nanociencia, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Romera
authors.affiliation: IMDEA-Nanociencia, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain.
authors.forename: Pierre
authors.initials: P
authors.lastname: Couleaud
authors.affiliation: IMDEA-Nanociencia, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain.
authors.forename: Sara H
authors.initials: SH
authors.lastname: Mejias
authors.affiliation: IMDEA-Nanociencia, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Aires
authors.affiliation: IMDEA-Nanociencia, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain Nanobiotecnología (IMDEA Nanociencia), Unidad Asociada al Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049 Madrid, Spain aitziber.lopezcortajarena@imdea.org.
authors.forename: Aitziber L
authors.initials: AL
authors.lastname: Cortajarena
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D016384:Consensus Sequence; D015723:Gene Library; D006801:Humans; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D011487:Protein Conformation; D015202:Protein Engineering; D017510:Protein Folding; D055550:Protein Stability; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D013698:Templates, Genetic
chemical_list: D001672:Biocompatible Materials; D010446:Peptide Fragments; D019151:Peptide Library; D011993:Recombinant Fusion Proteins
keywords: biomolecular templating; hybrid materials; nanostructures; protein design; repeat proteins; tetratricopeptide repeat