title: miR-150 inhibits terminal erythroid proliferation and differentiation.
abstract: MicroRNAs (miRNAs), a class of small non-coding linear RNAs, have been shown to play a crucial role in erythropoiesis. To evaluate the indispensable role of constant suppression of miR-150 during terminal erythropoiesis, we performed miR-150 gain- and loss-of-function experiments on hemin-induced K562 cells and EPO-induced human CD34+ cells. We found that forced expression of miR-150 suppresses commitment of hemoglobinization and CD235a labeling in both cell types. Erythroid proliferation is also inhibited via inducing apoptosis and blocking the cell cycle when miR-150 is overexpressed. In contrast, miR-150 inhibition promotes terminal erythropoiesis. 4.1 R gene is a new target of miR-150 during terminal erythropoiesis, and its abundance ensures the mechanical stability and deformability of the membrane. However, knockdown of 4.1 R did not affect terminal erythropoiesis. Transcriptional profiling identified more molecules involved in terminal erythroid dysregulation derived from miR-150 overexpression. These results shed light on the role of miR-150 during human terminal erythropoiesis. This is the first report highlighting the relationship between miRNA and membrane protein and enhancing our understanding of how miRNA works in the hematopoietic system.
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.affiliation: Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xielan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Minyuan
authors.initials: M
authors.lastname: Peng
authors.affiliation: Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Song
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Kunlu
authors.initials: K
authors.lastname: Wu
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Sun
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Biwei
authors.initials: B
authors.lastname: Qi
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Chufan
authors.initials: C
authors.lastname: Zhou
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.|Laboratory of Membrane Biology, New York Blood Center, New York, NY 10065, USA.
authors.forename: Xiuli
authors.initials: X
authors.lastname: An
authors.affiliation: The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410078, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D041905:Erythroid Cells / Q000166:cytology*; D004920:Erythropoiesis / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014161:Transduction, Genetic
chemical_list: D035683:MicroRNAs; C533097:MIR150, human
keywords: 4.1R; erythroid proliferation; miR-150; terminal erythropoiesis; transcriptional profiling