title: Pre-fractionation strategies to resolve pea (Pisum sativum) sub-proteomes.
abstract: Legumes are important crop plants and pea (Pisum sativum L.) has been investigated as a model with respect to several physiological aspects. The sequencing of the pea genome has not been completed. Therefore, proteomic approaches are currently limited. Nevertheless, the increasing numbers of available EST-databases as well as the high homology of the pea and medicago genome (Medicago truncatula Gaertner) allow the successful identification of proteins. Due to the un-sequenced pea genome, pre-fractionation approaches have been used in pea proteomic surveys in the past. Aside from a number of selective proteome studies on crude extracts and the chloroplast, few studies have targeted other components such as the pea secretome, an important sub-proteome of interest due to its role in abiotic and biotic stress processes. The secretome itself can be further divided into different sub-proteomes (plasma membrane, apoplast, cell wall proteins). Cell fractionation in combination with different gel-electrophoresis, chromatography methods and protein identification by mass spectrometry are important partners to gain insight into pea sub-proteomes, post-translational modifications and protein functions. Overall, pea proteomics needs to link numerous existing physiological and biochemical data to gain further insight into adaptation processes, which play important roles in field applications. Future developments and directions in pea proteomics are discussed.
authors.affiliation: Oxidative Stress and Plant Proteomics Group, Biocenter Klein Flottbek and Botanical Garden, University of Hamburg Hamburg, Germany ; Laboratoire de Biologie du Développement des Plantes, CEA, IBEB Saint-Paul-lez-Durance, France ; Centre National de la Recherche Scientifique, UMR 7265 Biologie Vegetale et Microbiologie Environnementales Saint-Paul-lez-Durance, France ; Aix Marseille Université, BVME UMR7265 Marseille, France.
authors.forename: Claudia-Nicole
authors.initials: CN
authors.lastname: Meisrimler
authors.affiliation: Oxidative Stress and Plant Proteomics Group, Biocenter Klein Flottbek and Botanical Garden, University of Hamburg Hamburg, Germany.
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Menckhoff
authors.affiliation: Faculty of Science and Mathematics, University of Banja Luka Banja Luka, Bosnia and Herzegovina.
authors.forename: Biljana M
authors.initials: BM
authors.lastname: Kukavica
authors.affiliation: Oxidative Stress and Plant Proteomics Group, Biocenter Klein Flottbek and Botanical Garden, University of Hamburg Hamburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Lüthje
keywords: Pisum sativum; apoplast; cell wall; chloroplast; mitochondria; plasma membrane