title: Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy.
abstract: The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detect Toxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological screening profiles for toxoplasmosis: unexposed (n = 65), probable acute infection (n = 48), possible acute infection (n = 58) and exposed to the parasite (n = 65). When an indirect immunofluorescence assay forT. gondii-specific IgG was considered as a reference test, the ELISA-rROP2 had a sensitivity of 61.8%, specificity of 62.8%, predictive positive value of 76.6% and predictive negative value of 45.4% (p = 0.0002). The ELISA-rROP2 reacted with 62.5% of the samples from pregnant women with probable acute infection and 40% of the samples from pregnant women with previous exposure (p = 0.0180). Seropositivity was observed in 50/57 (87.7%) pregnant women with possible infection. The results underscored that T. gondii rROP2 is recognised by specific IgG antibodies in both the acute and chronic phases of toxoplasmosis acquired during pregnancy. However, the sensitivity of the ELISA-rROP2 was higher in the pregnant women with probable and possible acute infections and IgM reactivity.
authors.affiliation: Departamento de Pediatria e Cirurgia Pediátrica, Centro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Jaqueline Dario
authors.initials: JD
authors.lastname: Capobiango
authors.affiliation: Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Sthefany
authors.initials: S
authors.lastname: Pagliari
authors.affiliation: Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Aline Kuhn Sbruzzi
authors.initials: AK
authors.lastname: Pasquali
authors.affiliation: Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Nino
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Fernanda Pinto
authors.initials: FP
authors.lastname: Ferreira
authors.affiliation: Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Thaís Cabral
authors.initials: TC
authors.lastname: Monica
authors.affiliation: Secretaria Municipal de Saúde de Cascavel, Cascavel, PR, Brasil.
authors.forename: Nely Norder
authors.initials: NN
authors.lastname: Tschurtschenthaler
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Italmar Teodorico
authors.initials: IT
authors.lastname: Navarro
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.affiliation: Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Regina
authors.initials: R
authors.lastname: Mitsuka-Breganó
authors.affiliation: Departamento de Patologia, Análises Clínicas e Toxicologia, Centro de Ciências da Saúde, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Edna Maria Vissoci
authors.initials: EM
authors.lastname: Reiche
mesh_terms: D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D016001:Confidence Intervals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000097:blood / Q000302:isolation & purification; D062069:Inventions / Q000592:standards; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D011237:Predictive Value of Tests; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008565:Membrane Proteins; D015800:Protozoan Proteins; C090555:ROP 2 protein, Toxoplasma gondii; D011994:Recombinant Proteins