title: An in vitro evaluation of the demineralization inhibitory effect of F(-) varnish and casein phosphopeptide-amorphous calcium phosphate on enamel in young permanent teeth.
abstract: OBJECTIVE
  To determine the demineralization inhibitory potential of fluoride varnish and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and to compare and evaluate the additive effect of fluoride varnish + CPP-ACP.
  
  
  METHODS
  Ten healthy premolar teeth that were extracted for orthodontic purposes were collected, and each tooth was longitudinally sectioned buccolingually and mesiodistally into four sections. The teeth were then assigned to four different treatment groups namely fluoride varnish, CPP-ACP, F(-) varnish followed by CPP-ACP and control. The prepared enamel samples were suspended in an artificial caries challenge for 10 days. The demineralizing inhibitory effects of the groups were recorded using polarized light microscopy.
  
  
  METHODS
  Statistical analysis was carried out using analysis of variance and Duncan's multiple range tests.
  
  
  RESULTS
  The mean lesion depths of all the groups were Group 1 (fluoride varnish): 104.71, Group 2 (CPP-ACP): 127.09, Group 3: (F(-) varnish + CPP-ACP): 82.34, Group 4 (control): 146.93.
  
  
  CONCLUSIONS
  Demineralization inhibitory potential on the additive use of F(-) varnish and casein phosphopeptide was superior to fluoride varnish or CPP-ACP applied alone on the enamel of young permanent teeth.
authors.affiliation: Department of Pedodontics, VMSDC, Salem, India.
authors.forename: Vinola
authors.initials: V
authors.lastname: Duraisamy
authors.affiliation: CSI Dental College, Madurai, Tamil Nadu, India.
authors.forename: Ananda
authors.initials: A
authors.lastname: Xavier
authors.affiliation: NIMS Dental College, Jaipur, Rajasthan, India.
authors.forename: Ullal Anand
authors.initials: UA
authors.lastname: Nayak
authors.affiliation: Mamatha Dental College, Khammam, Telangana, India.
authors.forename: Venugopal
authors.initials: V
authors.lastname: Reddy
authors.affiliation: Department of Pedodontics, MGPI, Puducherry, India.
authors.forename: Arun Prasad
authors.initials: AP
authors.lastname: Rao
keywords: Casein phosphopeptide-amorphous calcium phosphate; F-varnish; demineralization