title: Proteolysis of His-Phe-Arg-Trp-Pro-Gly-Pro in the blood and brain of rats in vivo.
abstract: The kinetics of the content of His-Phe-Arg-Trp-Pro-Gly-Pro (ACTH (6-9)PGP) and its hydrolysis products in the blood and brain of rats in the case of intranasal administration and intravenous injection of tritiated ACTH(6-9)PGP was studied. The parameters of bioavailability of ACTH(6-9)PGP administered intranasally were higher, indicating certain prospects in the intranasal application in clinical practice. We also found that the factor that determines ACTH(6-9)PGP proteolysis in experiments both in vivo and in vitro is aminopeptidases. The main products of ACTH(6-9)PGP during its metabolism in rats are short peptides and amino acids.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia. ATRegister@mail.ru.
authors.forename: K V
authors.initials: KV
authors.lastname: Shevchenko
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: I Yu
authors.initials: IY
authors.lastname: Nagaev
authors.affiliation: Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Biomedical Agency of Russia, Kapitolovo, Leningrad oblast, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Babakov
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: L A
authors.initials: LA
authors.lastname: Andreeva
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Shevchenko
authors.affiliation: Research Institute of Hygiene, Occupational Pathology, and Human Ecology, Federal Biomedical Agency of Russia, Kapitolovo, Leningrad oblast, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Radilov
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Myasoedov
mesh_terms: D000281:Administration, Intranasal; D061605:Administration, Intravenous; D000324:Adrenocorticotropic Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002491:Central Nervous System Agents / Q000008:administration & dosage / Q000378:metabolism*; D004355:Drug Stability; D007700:Kinetics; D010446:Peptide Fragments / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D059748:Proteolysis; D051381:Rats; D013997:Time Factors
chemical_list: D002491:Central Nervous System Agents; C000609775:His-Phe-Arg-Trp-Pro-Gly-Pro; D010446:Peptide Fragments; D000324:Adrenocorticotropic Hormone