title: The Quantified Level of Circulating Prostate Stem Cell Antigen mRNA relative to GAPDH Level Is a Clinically Significant Indictor for Predicting Biochemical Recurrence in Prostate Cancer Patients after Radical Prostatectomy.
abstract: The study quantified the relative absolute PSCA level in relation to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) level in the peripheral blood of 478 hormone-naive prostate cancer (PC) patients who underwent radical prostatectomy from 2005 to 2012 and evaluated its prognostic significance as a risk factor for predicting biochemical recurrence (BCR), compared to known parameters. Nested real-time polymerase chain reaction (RT-PCR) and gel electrophoresis detected PSCA levels and measured the PSCA/GAPDH ratio. Clinicopathological data from the institutional database were examined to determine the adequate cut-off level to predict postoperative BCR. A total of 110 patients had a positive PSCA result (23.0%) via RT-PCR (mean blood ratio 1.1 ± 0.4). The BCR was significantly higher in the PSCA-positive detection group (p = 0.009). A multivariate model was created to show that a PSCA/GAPDH ratio between 1.0 and 1.5 (HR 12.722), clinical T2c stage (HR 0.104), preoperative PSA (HR 1.225), extraprostatic capsule extension (HR 0.006), lymph node dissection (HR 16.437), and positive resection margin (HR 27.453) were significant predictive factors for BCR (p < 0.05). The study showed successful quantification of PSCA with its significance for BCR-related risk factor; however, further studies are needed to confirm its clinical predictive value.
authors.affiliation: Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Pathology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Weon Seo
authors.initials: WS
authors.lastname: Park
authors.affiliation: Genitourinary Cancer Branch, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Division of Specific Organ Cancer, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Moon Kyung
authors.initials: MK
authors.lastname: Choi
authors.affiliation: Biometric Research Branch, National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Yeon
authors.affiliation: Biometric Research Branch, National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Jeong Nam
authors.initials: JN
authors.lastname: Joo
authors.affiliation: Genitourinary Cancer Branch, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Ara
authors.initials: A
authors.lastname: Ko
authors.affiliation: Department of Urology, Seoul National University Hospital of Medicine, Seoul 110-744, Republic of Korea.
authors.forename: Eun Sik
authors.initials: ES
authors.lastname: Lee
authors.affiliation: Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Joung
authors.affiliation: Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Ho Kyung
authors.initials: HK
authors.lastname: Seo
authors.affiliation: Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Jinsoo
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Urology, Center for Prostate Cancer, Research Institute and Hospital of National Cancer Center, Goyang 410-769, Republic of Korea.
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D018450:Disease Progression; D058851:GPI-Linked Proteins / Q000097:blood; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000097:blood*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D015999:Multivariate Analysis; D009363:Neoplasm Proteins / Q000097:blood*; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis*; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D011468:Prostatectomy / Q000379:methods; D011471:Prostatic Neoplasms / Q000097:blood* / Q000601:surgery; D012333:RNA, Messenger / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012307:Risk Factors
chemical_list: D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins; C110843:PSCA protein, human; D012333:RNA, Messenger; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)