title: KH domain protein RCF3 is a tissue-biased regulator of the plant miRNA biogenesis cofactor HYL1.
abstract: The biogenesis of microRNAs (miRNAs), which regulate mRNA abundance through posttranscriptional silencing, comprises multiple well-orchestrated processing steps. We have identified the Arabidopsis thaliana K homology (KH) domain protein REGULATOR OF CBF GENE EXPRESSION 3 (RCF3) as a cofactor affecting miRNA biogenesis in specific plant tissues. MiRNA and miRNA-target levels were reduced in apex-enriched samples of rcf3 mutants, but not in other tissues. Mechanistically, RCF3 affects miRNA biogenesis through nuclear interactions with the phosphatases C-TERMINAL DOMAIN PHOSPHATASE-LIKE1 and 2 (CPL1 and CPL2). These interactions are essential to regulate the phosphorylation status, and thus the activity, of the double-stranded RNA binding protein and DICER-LIKE1 (DCL1) cofactor HYPONASTIC LEAVES1 (HYL1).
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Patricia
authors.initials: P
authors.lastname: Karlsson
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Michael Danger
authors.initials: MD
authors.lastname: Christie
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Danelle K
authors.initials: DK
authors.lastname: Seymour
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Jörg
authors.initials: J
authors.lastname: Hagmann
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany;
authors.forename: Franceli
authors.initials: F
authors.lastname: Kulcheski
authors.affiliation: Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany; Instituto de Agrobiotecnología del Litoral, Universidad Nacional del Litoral - Consejo Nacional de Investigaciones Científicas y Técnicas, 3000 Santa Fe, Argentina pablomanavella@ial.santafe-conicet.gov.ar.
authors.forename: Pablo Andrés
authors.identifier: http://orcid.org/0000-0003-3589-4414
authors.initials: PA
authors.lastname: Manavella
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D008156:Luciferases; D035683:MicroRNAs / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019076:Transgenes / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; C420966:HYL1 protein, Arabidopsis; D035683:MicroRNAs; C584805:RCF3 protein, Arabidopsis; D016601:RNA-Binding Proteins; D008156:Luciferases
keywords: Arabidopsis thaliana; HYL1; gene silencing; micro RNA biogenesis; phosphorylation