title: Apigenin, a potent suppressor of dendritic cell maturation and migration, protects against collagen-induced arthritis.
abstract: This study aimed to investigate whether apigenin (API) suppresses arthritis development through the modulation of dendritic cell functions. Bone marrow-derived dendritic cells (BMDCs) were stimulated in vitro with lipopolysaccharide (LPS) and treated with API for 24 hrs; DC functions, including phenotype expressions, cytokine secretion, phagocytosis and chemotaxis, were then investigated. The effects of API on collagen-induced arthritis (CIA) were examined in vivo, and purified DCs from the lymph nodes (LNs) of API-treated CIA mice were analysed for phenotypes and subsets. In in vitro, API efficiently restrained the phenotypic and functional maturation of LPS-stimulated BMDCs while maintaining phagocytotic capabilities. Moreover, API inhibited the chemotactic responses of LPS-stimulated BMDCs, which may be related to the depressive effect on chemokine receptor 4 (CXCR4). In in vivo, API treatment delayed the onset and reduced the severity of arthritis in CIA mice, and diminished secretion of pro-inflammatory cytokines in the serum and supernatants from the LN cells of the CIA mice. Similar to the in vitro findings, the API-treated mice exhibited reduced expression of co-stimulatory molecules and major histocompatibility complex II on DCs. Furthermore, API treatment strongly down-regulated the number of Langerhans cells, but not plasmacytoid DCs (pDCs) in LNs, which may be related to the depressive effect of API on the expression of CXCR4 on DCs of peripheral blood. These data provide new insight into the mechanism of action of API on arthritis and indicate that the inhibition of maturation and migration of DCs by API may contribute to its immunosuppressive effects.
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.|Hospital of South China Normal University, Guangzhou, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Qingyou
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Jie
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Jiaochan
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.affiliation: Key Laboratory of Proteomics of Guangdong Province, Department of Pathophysiology, Southern Medical University, Guangzhou, China.|State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Rheumatology and Immunology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.|Institute of Clinical Immunology, Academy of Orthopedics, Guangdong Province, China.
authors.forename: Erwei
authors.initials: E
authors.lastname: Sun
mesh_terms: D000818:Animals; D047310:Apigenin / Q000494:pharmacology* / Q000627:therapeutic use; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D024043:Collagen Type II / Q000276:immunology; D016207:Cytokines / Q000097:blood; D003713:Dendritic Cells / Q000187:drug effects / Q000502:physiology*; D007166:Immunosuppressive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008297:Male; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D019718:Receptors, CXCR4 / Q000097:blood
chemical_list: C514936:CXCR4 protein, mouse; D024043:Collagen Type II; D016207:Cytokines; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D019718:Receptors, CXCR4; D047310:Apigenin
keywords: apigenin; dendritic cells; maturation; migration; rheumatoid arthritis