title: Time-Lapse Fluorescence Microscopy of Budding Yeast Cells.
abstract: The discovery of green fluorescent protein (GFP) allowed visualization of a wide variety of processes within living cells. Thanks to the development of differently colored fluorophores, it is now possible to simultaneously follow distinct subcellular events at the single cell level. Here, we describe a basic method to visualize multiple events during cytokinesis by time-lapse fluorescence microscopy in the budding yeast Saccharomyces cerevisiae. In this organism, contraction of an actomyosin-based ring drives ingression of the plasma membrane at the mother-bud division site to partition the cytoplasm of the dividing cell. Simultaneous visualization of distinct cytokinesis steps in living cells, such as ring contraction and membrane ingression, will facilitate a complete understanding of the mechanisms of eukaryotic cell division.
authors.affiliation: Centre for Genomic Regulation (CRG), Dr. Aiguader 88, Barcelona, 08003, Spain.|Universitat Pompeu Fabra (UPF), Barcelona, 08003, Spain.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Centre for Genomic Regulation (CRG), Dr. Aiguader 88, Barcelona, 08003, Spain. manuel.mendoza@crg.es.|Universitat Pompeu Fabra (UPF), Barcelona, 08003, Spain. manuel.mendoza@crg.es.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mendoza
mesh_terms: D048749:Cytokinesis; D008856:Microscopy, Fluorescence / Q000379:methods; D004718:Saccharomycetales / Q000166:cytology*; D059008:Time-Lapse Imaging / Q000379:methods
keywords: Budding yeast; Cytokinesis; Fluorescence microscopy; Time-lapse