title: [Analysis of Human Platelet Antigen-1 System Alloantibodies Using Recombinant GPIIIa Fragments Coupled to Luminex Beads].
abstract: OBJECTIVE
  To detect platelet anti-HPA-1a and -1b antibodies using recombinant GPIIIa fragments coupled to Luminex beads.
  
  
  METHODS
  The sensitivity of 2 techniques, monoclonal antibody specific immobilization of platelet antigen (MAIPA) and Luminex bead assay, was compared using 12 twofold-serial dilutions (from neat to 1 in 2048) of an anti-HPA-1a WHO international standard. The specificity of Luminex assay to identify anti-HPA-1a and -1b antibodies was assessed using 8 negative or positive controls and 36 blinded samples provided by WHO Platelet Workshop.
  
  
  RESULTS
  The sensitivity of MAIPA and Luminex bead assay to detect anti-HPA-1a was dilution 1/64 (i.e. 1.56 IU/ml) and far more than dilution 1/2048 (i.e. 0.049 IU/mL), respectively. The Luminex bead assay could specifically identify negative and positive controls of anti-HPA-1a and -1b. All results of 36 blinded samples by Luminex assay were accordant to reference results except one sample which contained high concentration antithetical antibody and resulted in false positive of anti-HPA-1b. Cross-reactivity was also not observed with the samples containing HLA, ABO or other platelet antibodies.
  
  
  CONCLUSIONS
  The Luminex beads coupled with recombinant GPIIIa fragments can be used to detect HPA-1 system antibodies with sufficient sensitivity and specificity, that is suitable for the detection of platelet alloantibodies in clinical alloimmune thrombocytopenia.
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Xu
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Hong
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Su-Dan
authors.initials: SD
authors.lastname: Tao
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Kai-Rong
authors.initials: KR
authors.lastname: Ma
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Lan
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Ji
authors.initials: J
authors.lastname: He
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China. E-mail: yjs001@163.com.
authors.forename: Fa-Ming
authors.initials: FM
authors.lastname: Zhu
authors.affiliation: Institute of Transfusion Medicine, Blood Center of Zhejiang Province;Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, Zhejiang Province, China.
authors.forename: Hang-Jun
authors.initials: HJ
authors.lastname: Lyu
mesh_terms: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet / Q000276:immunology*; D001792:Blood Platelets; D006801:Humans; D039661:Integrin beta3 / Q000737:chemistry*; D007518:Isoantibodies / Q000097:blood*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000175:diagnosis*; D011994:Recombinant Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; D011994:Recombinant Proteins