title: In vitro selection of RNA aptamers against CA125 tumor marker in ovarian cancer and its study by optical biosensing.
abstract: Early identification of neoplastic diseases is essential to achieve timely therapeutic interventions and significantly reduce the mortality of patients. A well-known biomarker is the Cancer Antigen 125 (CA125) or 16 mucin (MUC 16), a glycoprotein of the human family of mucins, already used for the diagnostic and prognostic evaluation of ovarian cancer. Therefore, the detection of CA125 to now remains a promising tool in the early diagnosis of this tumor. In this paper, we describe the development of RNA aptamers that bind with high affinity the tumor antigen CA125. We performed eight cycles of selection against CA125 purified protein. The selected aptamers were cloned and sequenced and the binding properties of the most promising sequences were studied by Real Time PCR and Surface Plasmon Resonance (SPR) to evaluate their ability in targeting CA125 protein with perspective applications in aptamer-based bioassays.
authors.affiliation: Università di Roma Tre, Dipartimento di Scienze, Viale G. Marconi 446, 00146 Roma, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Lamberti
authors.affiliation: Università di Firenze, Dipartimento di Chimica "Ugo Schiff", Via della Lastruccia, 3-13, 50019 Sesto F.no (FI), Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Scarano
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto per l'Endocrinologia e Oncologia Molecolare "G. Salvatore", Via T. De Amicis 95, 80131 Napoli, Italy.
authors.forename: Carla Lucia
authors.initials: CL
authors.lastname: Esposito
authors.affiliation: Università di Roma Tre, Dipartimento di Scienze, Viale G. Marconi 446, 00146 Roma, Italy; INBB, Viale Medaglie d'oro 305, 00136 Roma, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Antoccia
authors.affiliation: Università di Roma Tre, Dipartimento di Scienze, Viale G. Marconi 446, 00146 Roma, Italy; INBB, Viale Medaglie d'oro 305, 00136 Roma, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Antonini
authors.affiliation: Università di Roma Tre, Dipartimento di Scienze, Viale G. Marconi 446, 00146 Roma, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Tanzarella
authors.affiliation: Consiglio Nazionale delle Ricerche, Istituto per l'Endocrinologia e Oncologia Molecolare "G. Salvatore", Via T. De Amicis 95, 80131 Napoli, Italy. Electronic address: defranci@unina.it.
authors.forename: Vittorio
authors.initials: V
authors.lastname: De Franciscis
authors.affiliation: INBB, Viale Medaglie d'oro 305, 00136 Roma, Italy; Università di Firenze, Dipartimento di Chimica "Ugo Schiff", Via della Lastruccia, 3-13, 50019 Sesto F.no (FI), Italy. Electronic address: maria.minunni@unifi.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Minunni
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D018394:CA-125 Antigen / Q000737:chemistry*; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D055029:Inverted Repeat Sequences; D008565:Membrane Proteins / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000175:diagnosis*; D011485:Protein Binding; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D018394:CA-125 Antigen; D055767:Immobilized Proteins; C451759:MUC16 protein, human; D008565:Membrane Proteins
keywords: Aptamers; CA125; SELEX; Surface Plasmon Resonance