title: Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.
abstract: Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling painted turtles exposed to 20 h freezing. Furthermore, we also explore the possibility of unique temperature-sensitive microRNA targeting programs that aid in adapting turtles for survival in the frozen state. Interestingly, two freeze-responsive 'cryo-miRs' (cpm-miR-16 and cpm-miR-21) were found to have unique low-temperature mRNA targets enriched in biological processes that are known to be part of the stress response.
authors.affiliation: Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada.
authors.forename: Kyle K
authors.initials: KK
authors.lastname: Biggar
authors.affiliation: Institute of Biochemistry & Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada. Electronic address: kenneth_storey@carleton.ca.
authors.forename: Kenneth B
authors.initials: KB
authors.lastname: Storey
mesh_terms: D000818:Animals; D058639:Cold-Shock Response / Q000235:genetics*; D004734:Energy Metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics*; D014426:Turtles / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*
chemical_list: D035683:MicroRNAs
keywords: Cryo-mIR; Hypometabolism; Metabolic rate depression; miR-16; miR-21