title: Bioremediation of petroleum hydrocarbon contaminated soil by Rhodobacter sphaeroides biofertilizer and plants.
abstract: Bio-augmentation is a promising technique for remediation of polluted soils. This study aimed to evaluate the bio-augmentation effect of Rhodobacter sphaeroides biofertilizer (RBF) on the bioremediation of total petroleum hydrocarbons (TPH) contaminated soil. A greenhouse pot experiment was conducted over a period of 120 days, three methods for enhancing bio-augmentation were tested on TPH contaminated soils, including single addition RBF, planting, and combining of RBF and three crop species, such as wheat (W), cabbage (C) and spinach (S), respectively. The results demonstrated that the best removal of TPH from contaminated soil in the RBF bio-augmentation rhizosphere soils was found to be 46.2%, 65.4%, 67.5% for W+RBF, C+RBF, S+RBF rhizosphere soils respectively. RBF supply impacted on the microbial community diversity (phospholipid fatty acids, PLFA) and the activity of soil enzymes, such as dehydrogenase (DH), alkaline phosphatase (AP) and urease (UR). There were significant difference among the soil only containing crude oil (CK), W, C and S rhizosphere soils and RBF bio-augmentation soils. Moreover, the changes were significantly distinct depended on crops species. It was concluded that the RBF is a valuable material for improving effect of remediation of TPH polluted soils.
authors.affiliation: Changzhi University, Changzhi, Shanxi, China / Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China / School of Chemical and Environmental Engineering, China University of Mining and Technology-Beijing, Beijing, China.
authors.forename: Haihua
authors.initials: H
authors.lastname: Jiao
authors.affiliation: Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jinxue
authors.initials: J
authors.lastname: Luo
authors.affiliation: Tianjin Normal University, Tianjin, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Bai
authors.affiliation: Changzhi University, Changzhi, Shanxi, China.
authors.forename: Zhanbin
authors.initials: Z
authors.lastname: Huang
mesh_terms: D001673:Biodegradation, Environmental; D001937:Brassica / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005308:Fertilizers; D006838:Hydrocarbons / Q000032:analysis*; D010578:Petroleum / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D010944:Plants / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis*; D018724:Spinacia oleracea / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D005308:Fertilizers; D006838:Hydrocarbons; D010578:Petroleum; D010743:Phospholipids; D012989:Soil Pollutants