title: Toxicological Implications and Inflammatory Response in Human Lymphocytes Challenged with Oxytetracycline.
abstract: Antibiotics are widely used in zoo technical and veterinary practices as feed supplementation to ensure wellness of farmed animals and livestock. Several evidences have been suggesting both the toxic role for tetracyclines, particularly for oxytetracycline (OTC). This potential toxicity appears of great relevance for human nutrition and for domestic animals. This study aimed to extend the evaluation of such toxicity. The biologic impact of the drug was assessed by evaluating the proinflammatory effect of OTC and their bone residues on cytokine secretion by in vitro human peripheral blood lymphocytes. Our results showed that both OTC and OTC-bone residues significantly induced the T lymphocyte and non-T cell secretion of interferon (IFN)-γ, as cytokine involved in inflammatory responses in humans as well as in animals. These results may suggest a possible implication for new potential human and animal health risks depending on the entry of tetracyclines in the food-processing chain.
authors.affiliation: School of Specialization in Clinical Biochemistry, "G. d'Annunzio" University, Chieti, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Di Cerbo
authors.affiliation: PhD School of Science, University of Basilicata, 85100, Potenza, Italy.
authors.forename: A T
authors.initials: AT
authors.lastname: Palatucci
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy.|Research and Development Laboratory, GRAF SpA, Nonantola, (MO), Italy.
authors.forename: V
authors.initials: V
authors.lastname: Rubino
authors.affiliation: Division of Research and Development, Sanypet SpA, 35023, Bagnoli di Sopra, (PD), Italy.
authors.forename: S
authors.initials: S
authors.lastname: Centenaro
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy.|Research and Development Laboratory, GRAF SpA, Nonantola, (MO), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Giovazzino
authors.affiliation: Division of Research and Development, Sanypet SpA, 35023, Bagnoli di Sopra, (PD), Italy.
authors.forename: E
authors.initials: E
authors.lastname: Fraccaroli
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cortese
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Ruggiero
authors.affiliation: Division of Research and Development, Sanypet SpA, 35023, Bagnoli di Sopra, (PD), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Guidetti
authors.affiliation: Division of Research and Development, Sanypet SpA, 35023, Bagnoli di Sopra, (PD), Italy.
authors.forename: S
authors.initials: S
authors.lastname: Canello
authors.affiliation: Department of Translational Medical Sciences, University of Naples Federico II, Naples, Italy. terrazza@unina.it, giuseppe.terrazzano@unibas.it.|Department of Science, University of Basilicata, 85100, Potenza, Italy. terrazza@unina.it, giuseppe.terrazzano@unibas.it.
authors.forename: G
authors.initials: G
authors.lastname: Terrazzano
mesh_terms: D017209:Apoptosis; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D008214:Lymphocytes / Q000187:drug effects*; D010118:Oxytetracycline / Q000633:toxicity*
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D010118:Oxytetracycline
keywords: Apoptosis; Inflammatory cytokine; Oxytetracycline; Toxicity; food