title: A novel vasorelaxant lectin purified from seeds of Clathrotropis nitida: partial characterization and immobilization in chitosan beads.
abstract: A novel lectin from seeds of Clathrotropis nitida (CNA) was purified and characterized. CNA is a glycoprotein containing approximately 3.3% carbohydrates in its structure. CNA promoted intense agglutination of rabbit erythrocytes, which was inhibited by galactosides and porcine stomach mucin (PSM). The lectin maintained its hemagglutinating activity after incubation in a wide range of temperatures (30-60 °C) and pH (6.0-7.0), and its binding activity was dependent on divalent cations (Ca(+2) and Mg(+2)). SDS-PAGE showed an electrophoretic profile consisting of a single band of 28 kDa, as confirmed by electrospray ionization mass spectrometry, which indicated an average molecular mass of 27,406 ± 2 Da and the possible presence of isoforms and glycoforms. In addition, CNA exhibited no toxicity to Artemia sp. nauplii and elicited reversible and dose-dependent vasorelaxation in precontracted aortic rings. CNA was successfully immobilized on chitosan beads and was able to capture PSM in solution. This study demonstrated that CNA is a lectin that has potential as a biotechnological tool in glycomics and glycoproteomics applications.
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Ana Cecilia
authors.initials: AC
authors.lastname: Alves
authors.affiliation: Laboratório Integrado de Biomoléculas - LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Mayron Alves
authors.initials: MA
authors.lastname: Vasconcelos
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Mayara Queiroz
authors.initials: MQ
authors.lastname: Santiago
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Vanir Reis
authors.initials: VR
authors.lastname: Pinto-Junior
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Vinicius Jose
authors.initials: VJ
authors.lastname: Silva Osterne
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Claudia Figueiredo
authors.initials: CF
authors.lastname: Lossio
authors.affiliation: Instituto Superior de Ciências Biomédicas-ISCB and Faculdade de Filosofia Dom Aureliano Matos-FAFIDAM, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Pedro Henrique Souza Ferreira
authors.initials: PH
authors.lastname: Bringel
authors.affiliation: Instituto Superior de Ciências Biomédicas-ISCB and Faculdade de Filosofia Dom Aureliano Matos-FAFIDAM, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Rondinelle Ribeiro
authors.initials: RR
authors.lastname: Castro
authors.affiliation: Laboratório de Espectrometria de Massas Aplicada a Proteínas - LEMAP, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.affiliation: Departamento de Biologia Molecular, Centro de Ciências Exatas e da Natureza - Campus I, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.affiliation: Instituto Nacional de Pesquisas da Amazônia-INPA, Manaus, Amazonas, Brazil.
authors.forename: Luiz Augusto Gomes
authors.initials: LA
authors.lastname: Souza
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: Nascimento
authors.affiliation: Instituto Superior de Ciências Biomédicas-ISCB and Faculdade de Filosofia Dom Aureliano Matos-FAFIDAM, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.affiliation: Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil. Electronic address: bscavada@ufc.br.
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology; D001156:Artemia / Q000187:drug effects; D048271:Chitosan; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics; D006384:Hemagglutination / Q000187:drug effects; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014665:Vasodilator Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D055767:Immobilized Proteins; D037121:Plant Lectins; D014665:Vasodilator Agents; D048271:Chitosan
keywords: Clathrotropis nitida; Immobilization; Lectin; Purification; Toxicity; Vasorelaxation