title: Generation and Characterization of Mouse Regulatory Macrophages.
abstract: In the last years, cell therapy has become a promising approach to therapeutically manipulate immune responses in autoimmunity, cancer, and transplantation. Several types of lymphoid and myeloid cells origin have been generated in vitro and tested in animal models. Their efficacy to decrease pharmacological treatment has successfully been established. Macrophages play an important role in physiological and pathological processes. They represent an interesting cell population due to their high plasticity in vivo and in vitro. Here, we describe a protocol to differentiate murine regulatory macrophages in vitro from bone marrow precursors. We also describe several methods to assess macrophage classical functions, as their bacterial killing capacity and antigen endocytosis and degradation. Importantly, regulatory macrophages also display suppressive characteristics, which are addressed by the study of their hypostimulatory T lymphocyte capacity and polyclonal T lymphocyte activation suppression.
authors.affiliation: ITUN, Inserm UMRS 1064, Center for Research in Transplantation and Immunology, Nantes, France. Laura.Carretero-Iglesia@univ-nantes.fr.
authors.forename: Laura
authors.initials: L
authors.lastname: Carretero-Iglesia
authors.affiliation: Laboratorio de Inmunorregulación e inflamación, Departamento de Inmunobiología, Facultad de Medicina, Institut Pasteur de Montevideo, Universidad de la Republica, Montevideo, Uruguay.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Hill
authors.affiliation: ITUN, Inserm UMRS 1064, Center for Research in Transplantation and Immunology, Nantes, France. Maria-Cristina.Cuturi@univ-nantes.fr.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Cuturi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D004705:Endocytosis / Q000276:immunology; D056747:Immunomodulation; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D055017:Monocyte-Macrophage Precursor Cells / Q000166:cytology
keywords: Bacterial killing assay (BKA); Cell therapy; In vitro differentiation; Regulatory macrophages (Mreg); Suppression