title: Investigation of Antiulcer and Antioxidant Activity of Juniperus phoenicea L. (1753) Essential Oil in an Experimental Rat Model.
abstract: Juniperus phoenicea is a tree of the Cupressaceae family that is popularly known in the south of Tunisia because of its wide application in herbal medicine, including the use of its leaves to treat many diseases such as diarrhea, rheumatism, and intestinal disorders. The aim of this study was to evaluate the ulceroprotective and antioxidant activity of essential oil extracted from the leaves of J. phoenicea (EOJp) against hydrogen chloride (HCl)/ethanol-induced ulcers in rats. The antiulcer activities of 50, 75 and 100 mg/kg body weight (b.w.) EOJp were investigated on 0.3 M HCl/ethanol-induced ulcers in rats. The essential oil yield was 0.69% with 48 compounds; α-pinene was the principal component (20.24%). In vivo pretreatment with EOJp given orally provided dose-dependent protection against HCl/ethanol-induced gastric ulcers in rats. Furthermore, pretreatment with EOJp significantly decreased malondialdehyde (MDA) content and increased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). The activity of the antiulcerogenic EOJp could be from synergistic antioxidant and anti-secretory effects. Oral use of EOJp has excellent preventive effects on induced gastric ulcers comparable to those of the proton pump inhibitor (PPI) omeprazole.
authors.affiliation: Laboratory of Environment Biomonitoring (LR01/ES14), Faculty of Sciences of Bizerte, University of Carthage.
authors.forename: Manel Jemaї
authors.initials: MJ
authors.lastname: Ben Ali
authors.forename: Fatma
authors.initials: F
authors.lastname: Guesmi
authors.forename: Abdel Halim
authors.initials: AH
authors.lastname: Harrath
authors.forename: Saleh
authors.initials: S
authors.lastname: Alwasel
authors.forename: Amor
authors.initials: A
authors.lastname: Hedfi
authors.forename: Sana
authors.initials: S
authors.lastname: Ncib
authors.forename: Ahmed
authors.initials: A
authors.lastname: Landoulsi
authors.forename: Badr
authors.initials: B
authors.lastname: Aldahmash
authors.forename: Mossadok
authors.initials: M
authors.lastname: Ben-Attia
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D000081008:Bicyclic Monoterpenes; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol; D005979:Glutathione Peroxidase / Q000378:metabolism; D006851:Hydrochloric Acid; D018653:Juniperus / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D039821:Monoterpenes / Q000494:pharmacology / Q000627:therapeutic use; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D000431:Ethanol; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C005451:alpha-pinene; D006851:Hydrochloric Acid