title: Strain Improvement of Streptomyces xanthochromogenes RIA 1098 for Enhanced Pravastatin Production at High Compactin Concentrations.
abstract: Pravastatin is one of the most popular cholesterol-lowering drugs. Its industrial production represents a two-stage process including the microbial production of compactin and its further biocatalytic conversion to pravastatin. To increase a conversion rate, a higher compactin content in fermentation medium should be used; however, high compactin concentrations inhibit microbial growth. Therefore, the improvement of the compactin resistance of a producer still remains a relevant problem. A multi-step random UV mutagenesis of a Streptomyces xanthochromogenes strain RIA 1098 and the further selection of high-yield compactin-resistant mutants have resulted in a highly productive compactin-resistant strain S 33-1. After the fermentation medium improvement, the maximum bioconversion rate of this strain has reached 91 % at the daily compactin dose equal to 1 g/L and still remained high (83 %) even at the doubled dose (2 g/L). A 1-year study of the mutant strain stability has proved a stable inheritance of its characteristics that provides this strain to be very promising for the pravastatin-producing industry.
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Vakhtang V
authors.initials: VV
authors.lastname: Dzhavakhiya
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Tatiana M
authors.initials: TM
authors.lastname: Voinova
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Glagoleva
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Petukhov
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Ovchinnikov
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Maksim I
authors.initials: MI
authors.lastname: Kartashov
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Boris B
authors.initials: BB
authors.lastname: Kuznetsov
authors.affiliation: Laboratory of Biotechnology of Physiologically Active Compounds, Center for Bioengineering, Russian Academy of Sciences, Prospekt 60-Letiya Oktyabrya, 7/1, Moscow, 117312 Russia.
authors.forename: Konstantin G
authors.initials: KG
authors.lastname: Skryabin
keywords: Bioconversion rate; Compactin resistance; Pravastatin; Streptomyces xanthochromogenes; UV mutagenesis