title: Evolution and characterization of a benzylguanine-binding RNA aptamer.
abstract: Repurposing the "protein-labeling toolkit" for RNA research could be a pragmatic approach for developing new RNA-labeling methods. We have evolved an RNA aptamer that tightly binds benzylguanine (bG), the key ligand for the protein SNAP-tag. The aptamer tightly binds bG fluorophores and can be purified from cellular RNA with bG agarose under native conditions.
authors.affiliation: Department of Biochemistry, U. Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA. ahoskins@wisc.edu.
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: T J
authors.initials: TJ
authors.lastname: Carrocci
authors.forename: A A
authors.initials: AA
authors.lastname: Hoskins
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D002847:Chromatography, Agarose / Q000379:methods; D006146:Guanidines / Q000737:chemistry*; D015394:Molecular Structure; D012313:RNA / Q000737:chemistry*; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D006146:Guanidines; C117872:benzylguanidine; D012313:RNA