title: Altered Hippocampal Lipid Profile Following Acute Postnatal Exposure to Di(2-Ethylhexyl) Phthalate in Rats.
abstract: Slight changes in the abundance of certain lipid species in the brain may drastically alter normal neurodevelopment via membrane stability, cell signalling, and cell survival. Previous findings have demonstrated that postnatal exposure to di (2-ethylhexyl) phthalate (DEHP) disrupts normal axonal and neural development in the hippocampus. The goal of the current study was to determine whether postnatal exposure to DEHP alters the lipid profile in the hippocampus during postnatal development. Systemic treatment with 10 mg/kg DEHP during postnatal development led to elevated levels of phosphatidylcholine and sphingomyelin in the hippocampus of female rats. There was no effect of DEHP exposure on the overall abundance of phosphatidylcholine or sphingomyelin in male rats or of lysophosphatidylcholine in male or female rats. Individual analyses of each identified lipid species revealed 10 phosphatidylcholine and six sphingomyelin lipids in DEHP-treated females and a single lysophosphatidylcholine in DEHP-treated males with a two-fold or higher increase in relative abundance. Our results are congruent with previous work that found that postnatal exposure to DEHP had a near-selective detrimental effect on hippocampal development in males but not females. Together, results suggest a neuroprotective effect of these elevated lipid species in females.
authors.affiliation: Department of Neuroscience, Carleton University, 1125 Colonel By Drive, 325 LSRB, Ottawa ON, K1S 5B6, Canada. catherine.smith1101@gmail.com.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Smith
authors.affiliation: Department of Neuroscience, Carleton University, 1125 Colonel By Drive, 325 LSRB, Ottawa ON, K1S 5B6, Canada. Kyle.Farmer@carleton.ca.
authors.forename: Kyle
authors.initials: K
authors.lastname: Farmer
authors.affiliation: Department of Chemistry, Carleton University, Ottawa, 1125 Colonel By Drive, SC-226, Ottawa, ON, K1S5B6, Canada. Hyunmin.Lee@carleton.ca.
authors.forename: Hyunmin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Neuroscience, Carleton University, 1125 Colonel By Drive, 325 LSRB, Ottawa ON, K1S 5B6, Canada. Matthew.Holahan@carleton.ca.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Holahan
authors.affiliation: Department of Chemistry, Carleton University, Ottawa, 1125 Colonel By Drive, SC-226, Ottawa, ON, K1S5B6, Canada. jeff.smith@carleton.ca.
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Smith
mesh_terms: D000818:Animals; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D006624:Hippocampus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D013109:Sphingomyelins / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D004051:Diethylhexyl Phthalate
keywords: Hippocampus; Lysophosphatidylcholine; mass spectrometry; phosphatidylcholine; phthalate; sphingolipids