title: Molecular cloning, expression analysis and transcript localization of testicular orphan nuclear receptor 2 in the male catfish, Clarias batrachus.
abstract: Testicular receptor 2 (TR2; also known as Nr2c1) is one of the first orphan nuclear receptors identified and known to regulate various physiological process with or without any ligand. In this study, we report the cloning of full length nr2c1 and its expression analysis during gonadal development, seasonal testicular cycle and after human chorionic gonadotropin (hCG) induction. In addition, in situ hybridization (ISH) was performed to localize nr2c1 transcripts in adult testis and whole catfish (1day post hatch). Tissue distribution and gonadal ontogeny studies revealed high expression of nr2c1 in developing and adult testis. Early embryonic stage-wise expression of nr2c1 seems to emphasize its importance in cellular differentiation and development. Substantial expression of nr2c1 during pre-spawning phase and localization of nr2c1 transcripts in sperm/spermatids were observed. Significant upregulation after hCG induction indicate that nr2c1 is under the regulation of gonadotropins. Whole mount ISH analysis displayed nr2c1 expression in notochord indicating its role in normal vertebrate development. Taken together, our findings suggest that nr2c1 may have a plausible role in the testicular and embryonic development of catfish.
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
authors.forename: R
authors.initials: R
authors.lastname: Murugananthkumar
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Akhila
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
authors.forename: A
authors.initials: A
authors.lastname: Rajakumar
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Mamta
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India.
authors.forename: C C
authors.initials: CC
authors.lastname: Sudhakumari
authors.affiliation: Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500046, Telangana, India. Electronic address: bsksl@uohyd.ernet.in.
authors.forename: B
authors.initials: B
authors.lastname: Senthilkumaran
mesh_terms: D000818:Animals; D002397:Catfishes / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian; D047108:Embryonic Development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1 / Q000235:genetics* / Q000378:metabolism*; D012621:Seasons; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D006063:Chorionic Gonadotropin; D057107:Nuclear Receptor Subfamily 2, Group C, Member 1
keywords: Catfish; Embryo; Nr2c1; Testicular cycle; Testis; hCG induction