title: Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients from two municipalities of the Jequitinhonha Valley, MG, Brazil, using two molecular typing strategies.
abstract: BACKGROUND
  Trypanosoma cruzi is classified into six discrete taxonomic units (DTUs). For this classification, different biological markers and classification criteria have been used. The objective was to identify the genetic profile of T. cruzi samples isolated from patients of two municipalities of Jequitinhonha Valley, MG, Brazil.
  
  
  METHODS
  Molecular characterization was performed using two different criteria for T. cruzi typing to characterize 63 T. cruzi samples isolated from chronic Chagas disease patients. The characterizations followed two distinct methodologies. Additionally, the RAPD technique was used to evaluate the existence of genetic intragroup variability.
  
  
  RESULTS
  The first methodology identified 89% of the samples as TcII, but it was not possible to define the genetic identity of seven isolates. The results obtained with the second methodology corroborated the classification as TcII of the same samples and defined the classification of the other seven as TcVI. RAPD analysis showed lower intra-group variability in TcII.
  
  
  CONCLUSIONS
  The results confirmed the preliminary data obtained in other municipalities of the Jequitinhonha Valley, showing a predominance of TcII, similar to that verified in northeast/south axis of Brazil and the first detection of TcVI in the study region. The second protocol was more simple and reliable to identify samples of hybrid character.
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto (UFOP), Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. maykontavares@yahoo.com.br.
authors.forename: Maykon Tavares
authors.initials: MT
authors.lastname: de Oliveira
authors.affiliation: Departamento- Básico de Saúde, Universidade Federal de Juiz de Fora (UFJF), CEP: 35010-177, Campus Governador Valadares, Governador Valadares, MG, Brazil. girley.francisco@ufjf.edu.br.
authors.forename: Girley Francisco Machado
authors.initials: GF
authors.lastname: de Assis
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto (UFOP), Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. jackvalamiel@yahoo.com.br.
authors.forename: Jaquelline Carla Valamiel
authors.initials: JC
authors.lastname: Oliveira e Silva
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto (UFOP), Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. evandro@ufop.br.
authors.forename: Evandro Marques Menezes
authors.initials: EM
authors.lastname: Machado
authors.affiliation: Departamento de Análises Clínicas, Escola de Farmácia, UFOP, CEP: 35400-000 Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. glenda_silva@yahoo.com.|Programa de Pós-Graduação em Ciências Farmacêuticas (CiPHARMA), Escola de Farmácia, UFOP, Campus Universitário Morro do Cruzeiro, 35400-000, Ouro Preto, MG, Brazil. glenda_silva@yahoo.com.
authors.forename: Glenda Nicioli
authors.initials: GN
authors.lastname: da Silva
authors.affiliation: Departamento de Análises Clínicas, Escola de Farmácia, UFOP, CEP: 35400-000 Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. vanjaveloso@gmail.com.|Programa de Pós-Graduação em Ciências Farmacêuticas (CiPHARMA), Escola de Farmácia, UFOP, Campus Universitário Morro do Cruzeiro, 35400-000, Ouro Preto, MG, Brazil. vanjaveloso@gmail.com.
authors.forename: Vanja Maria
authors.initials: VM
authors.lastname: Veloso
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), 6627, Belo Horizonte, 31270-901, MG, Brazil. andrea@icb.ufmg.br.
authors.forename: Andrea Mara
authors.initials: AM
authors.lastname: Macedo
authors.affiliation: Departamento de Farmácia, Faculdade de Ciências Biológicas e da Saúde, Universidade dos Vales do Jequitinhonha e Mucuri (UFVJM), 39100-000, Diamantina, MG, Brazil. helen.rmartins@gmail.com.
authors.forename: Helen Rodrigues
authors.initials: HR
authors.lastname: Martins
authors.affiliation: Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto (UFOP), Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. delana@nupeb.ufop.br.|Departamento de Análises Clínicas, Escola de Farmácia, UFOP, CEP: 35400-000 Campus Universitário Morro do Cruzeiro, CEP: 35400-000, Ouro Preto, MG, Brazil. delana@nupeb.ufop.br.|Programa de Pós-Graduação em Ciências Farmacêuticas (CiPHARMA), Escola de Farmácia, UFOP, Campus Universitário Morro do Cruzeiro, 35400-000, Ouro Preto, MG, Brazil. delana@nupeb.ufop.br.
authors.forename: Marta
authors.initials: M
authors.lastname: de Lana
mesh_terms: D001938:Brazil; D014355:Chagas Disease / Q000469:parasitology*; D002908:Chronic Disease; D002947:Cities; D005838:Genotype; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D014349:Trypanosoma cruzi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*