title: Nonylphenol exposure is associated with oxidative and nitrative stress in pregnant women.
abstract: Animal studies have shown that exposure to nonylphenol (NP) increases oxidative/nitrative stress, but whether it does so in humans is unknown. This study examines prenatal exposure to NP and its effects on oxidatively/nitratively damaged DNA, lipid peroxidation, and the activities of antioxidants. A total of 146 urine and blood specimens were collected during gestational weeks 27-38 and hospital admission for delivery, respectively. Urinary NP was analyzed by high-performance liquid chromatography (HPLC). Urinary biomarkers of oxidatively/nitratively damaged DNA and lipid peroxidation, including 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), 8-nitroguanine (8-NO(2)Gua), 8-iso-prostaglandin F(2α) (8-isoPF(2α)) and 4-hydroxy-2-nonenal-mercapturic acid (HNE-MA), were simultaneously analyzed using isotope-dilution liquid-chromatography/electron spray ionization tandem mass spectrometry. The activities of maternal plasma superoxide dismutase and glutathione peroxidase were analyzed by enzyme-linked immunosorbent assay. Urinary NP level was significantly associated with 8-oxodG and 8-NO(2)Gua levels in late pregnancy, suggesting that NP may enhance oxidatively and nitratively damaged DNA. The adjusted odds ratios for high 8-oxodG level exhibited a significantly dose-response relationship with NP levels, stratified into four quartiles. 8-oxodG appears to be a more sensitive and effective biomarker of NP exposure than 8-NO(2)Gua. These relationships suggest NP may play a role in the pregnancy complications.
authors.affiliation: a Department of Pediatrics , Taipei City Hospital, Heping Fuyou Branch , Taipei , Taiwan.
authors.forename: Pei-Wei
authors.initials: PW
authors.lastname: Wang
authors.affiliation: b Institute of Environmental and Occupational Health Sciences, National Yang Ming University , Taipei , Taiwan.
authors.forename: Mei-Lien
authors.initials: ML
authors.lastname: Chen
authors.affiliation: c Department of Obstetrics and Gynecology , Taipei City Hospital, Heping Fuyou Branch , Taipei , Taiwan.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Huang
authors.affiliation: d Division of Pediatrics , Taipei City Hospital, Yangming Branch , Taipei , Taiwan.
authors.forename: Winnie
authors.initials: W
authors.lastname: Yang
authors.affiliation: e Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University , Taipei , Taiwan.
authors.forename: Kuen-Yuh
authors.initials: KY
authors.lastname: Wu
authors.affiliation: b Institute of Environmental and Occupational Health Sciences, National Yang Ming University , Taipei , Taiwan.|f Department of Education and Research , Taipei City Hospital , Taipei , Taiwan.
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004249:DNA Damage / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D015538:Nitrosation; D018384:Oxidative Stress / Q000502:physiology; D010636:Phenols / Q000009:adverse effects*; D011247:Pregnancy; D053719:Tandem Mass Spectrometry
chemical_list: C095838:8-nitroguanine; D015415:Biomarkers; D010636:Phenols; D006147:Guanine; C025256:nonylphenol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-nitroguanine (8-NO2Gua); 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG); Lipid peroxidation; nonylphenol; oxidative/nitrative stress