title: Archaeal MCM Proteins as an Analog for the Eukaryotic Mcm2-7 Helicase to Reveal Essential Features of Structure and Function.
abstract: In eukaryotes, the replicative helicase is the large multisubunit CMG complex consisting of the Mcm2-7 hexameric ring, Cdc45, and the tetrameric GINS complex. The Mcm2-7 ring assembles from six different, related proteins and forms the core of this complex. In archaea, a homologous MCM hexameric ring functions as the replicative helicase at the replication fork. Archaeal MCM proteins form thermostable homohexamers, facilitating their use as models of the eukaryotic Mcm2-7 helicase. Here we review archaeal MCM helicase structure and function and how the archaeal findings relate to the eukaryotic Mcm2-7 ring.
authors.affiliation: Department of Structural Biology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.
authors.forename: Justin M
authors.initials: JM
authors.lastname: Miller
authors.affiliation: Department of Structural Biology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Enemark
mesh_terms: D001105:Archaea / Q000737:chemistry* / Q000201:enzymology*; D004265:DNA Helicases / Q000737:chemistry* / Q000378:metabolism*; D064110:Minichromosome Maintenance Proteins / Q000737:chemistry* / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D009097:Multienzyme Complexes; D055503:Protein Multimerization
chemical_list: D009097:Multienzyme Complexes; D004265:DNA Helicases; D064110:Minichromosome Maintenance Proteins