title: Matrix changes due to the toxic effects of metronidazole in intestinal tissue of fish (Onchorhynchus mykiss).
abstract: Metranidazole (MTZ) is an antibiotic used for parasitic infections in a number of species. Accumulation of this drug in the environment and its interaction with fish of economic value makes this drug particularly important. In the present study, we examined the histopathological effects of MTZ on the intestinal tissue of Oncorhynchus mykiss. The fish in aquarium were exposed to MTZ at doses of 5, 10, 20 mg/L for 2, 4 and 8 days. At the end of the experiments, macroscopic pathology or death were not observed at these doses. Histochemical staining with Haematoxylene-Eosin, Periodic Acid Schiff and Gomori Trichrome showed, depending on increased dose and prolonged duration, areas of necrosis, edema, inflammation, small tears at the tips of the villi and excretion with heterogenic distribution of the Goblet cells. Moreover, changes in the connective tissue of the intestines due to toxicity of MTZ and decreases in immunostaining of matrix proteins such as laminin and collagen IV, especially in the epithelium were observed. Findings of the present study would be useful to demonstrate the adverse effects of MTZ use, emphasizing the importance of the effect on fish which could be very important public health.
authors.affiliation: Celal Bayar University, School of Science and Letter, Department of Biology, Manisa, Turkey. Electronic address: byhgurcu@gmail.com.
authors.forename: Beyhan
authors.initials: B
authors.lastname: Gürcü
authors.affiliation: Adnan Menderes University, School of Science and Letter, Department of Biology, Aydın, Turkey. Electronic address: ybasimoglukoca@gmail.com.
authors.forename: Yücel Başımoğlu
authors.initials: YB
authors.lastname: Koca
authors.affiliation: Celal Bayar University, School of Medicine, Department of Histology and Embriology, Manisa, Turkey. Electronic address: mahmudozkut@yahoo.com.tr.
authors.forename: Mahmud
authors.initials: M
authors.lastname: Özkut
authors.affiliation: Celal Bayar University, School of Medicine, Department of Histology and Embriology, Manisa, Turkey. Electronic address: mituglu@yahoo.com.
authors.forename: Mehmet İbrahim
authors.initials: Mİ
authors.lastname: Tuğlu
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D054750:Ecotoxicology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D008795:Metronidazole / Q000633:toxicity*; D017686:Oncorhynchus mykiss / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D008795:Metronidazole
keywords: Connective tissue; Extracellular matrix; Intestine; Metronidazole; Onchorhynchus mykiss; Toxicity