title: Doxorubicin causes transient activation of protein poly(ADP-ribosyl)ation in H9c2 cardiomyocytes.
abstract: Possible involvement of the system of protein poly(ADP-ribosyl)ation in the mechanisms of cardiotoxicity of doxorubicin, one of the most frequently used anticancer drug, was studied in cultures of cardiomyocytes H9c2. The treatment of H9c2 cells with doxorubicin (1 µM) led to a transient (after 6 h of incubation) increase in the nuclear level of poly(ADP-ribosyl)ated proteins. The observed data indirectly indicate the development of genotoxic stress in the doxorubicin-treated cells, probably caused by the stimulatory effects of doxorubicin and its metabolites on the production of reactive oxygen and nitrogen species.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Efremova
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia. shram@img.ras.ru.
authors.forename: S I
authors.initials: SI
authors.lastname: Shram
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia.
authors.forename: N F
authors.initials: NF
authors.lastname: Myasoedov
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics / Q000633:toxicity*; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D004317:Doxorubicin / Q000493:pharmacokinetics / Q000633:toxicity*; D020536:Enzyme Activators / Q000493:pharmacokinetics / Q000633:toxicity*; D005455:Fluorescent Antibody Technique; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology*; D017711:Nonlinear Dynamics; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D020536:Enzyme Activators; D004317:Doxorubicin; D011065:Poly(ADP-ribose) Polymerases