title: PEG3 domain gene expression in maternal and foetal placenta in intrauterine growth restricted bovine foetuses.
abstract: We used a genetic (MIMT1(Del)) model of intrauterine growth restriction to investigate dysregulation of PEG3 domain gene expression in bovine foetal and maternal placenta. ZIM2, APEG3 and PEG3 expressions were similarly reduced in MIMT1(Del/) (WT) foetal placenta, suggesting coordinated regulation. Methylation of DNA CpG sites associated with these genes showed no differences, but differences in the levels of MIMT1 RNA methylation at three CpG sites were found in foetal placenta. Our data are consistent with the presence of a bidirectional promoter 5' of MIMT1 and suggest a regulatory role for the MIMT1 non-coding transcript. PEG3 domain expression on the maternal placenta side was not affected by the foetal mutation.
authors.affiliation: Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
authors.forename: Shun
authors.initials: S
authors.lastname: Li
authors.affiliation: Lehrstuhl für Tierzucht, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
authors.forename: Hubert
authors.initials: H
authors.lastname: Pausch
authors.affiliation: Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Paroninkuja 20, 04920, Saarentaus, Finland.
authors.forename: Heli
authors.initials: H
authors.lastname: Venhoranta
authors.affiliation: Chair of Forest Economics, Poznan University of Life Science, ul. Wojska Polskiego 71 C, 60-625, Poznan, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Adamowicz
authors.affiliation: Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Paroninkuja 20, 04920, Saarentaus, Finland.
authors.forename: Magnus
authors.initials: M
authors.lastname: Andersson
authors.affiliation: Department of Molecular Biology, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, ul. Postepu 36A, Jastrzebiec, 05-552, Magdalenka, Poland.
authors.forename: Lech
authors.initials: L
authors.lastname: Zwierzchowski
authors.affiliation: Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kind
authors.affiliation: Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
authors.forename: Angelika
authors.initials: A
authors.lastname: Schnieke
authors.affiliation: Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Liesel-Beckmannstr. 1, 85354, Freising, Germany.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Flisikowski
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics*; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D009154:Mutation; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011401:Promoter Regions, Genetic
keywords: DNA methylation; RNA methylation; cattle; intrauterine growth restriction; non-coding RNA; placental transcription