title: Overexpression of truncated AIF regulated by Egr1 promoter radiation-induced apoptosis on MCF-7 cells.
abstract: It has been demonstrated that gene-radiotherapy can improve the radiotherapy by selectively increasing cells' response to ionizing radiation. Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein, and its C-terminal domain is responsible for the proapoptotic activity. In the present study, we overexpressed truncated AIF on MCF-7 cells by transfection of pcDNA3.1-tAIF (pc-tAIF) and pcDNA3.1-Egr1-tAIF (pc-Egr1-tAIF) plasmids. After MCF-7-tAIF cells were exposed to X-rays, the AIF and tAIF expressions, cell proliferation, apoptosis, cell cycle invasion, cytochrome c (Cyt c) release and activation of caspase-9 were measured by using Western blot, MTT assay, flow cytometry and Matrigel transwell assay, respectively. Our results showed that tAIF expression increased on time- and dose-dependent manners. Both tAIF and radiation can synergistically enhance the apoptosis, cell proliferation inhibition, cell cycle arrest and cell-invasive inhibition. In addition, tAIF overexpression and irradiation increased Cyt c release. However, only irradiation increased caspase-9 activation. Our studies indicated that tAIF overexpression might enhance apoptosis induced by radiation in MCF-7 cells.
authors.affiliation: Department of Radiotherapy, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, People's Republic of China.|Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology and Otolaryngology, The Children's Hospital of Changchun, Changchun, Jilin Province, People's Republic of China.
authors.forename: Yana
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China.|School of Public Health and Family Medicine, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China.
authors.forename: Shouliang
authors.initials: S
authors.lastname: Gong
authors.affiliation: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China. 445422273@qq.com.|Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China. 445422273@qq.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, Jilin Province, People's Republic of China. zhicheng123a@126.com.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D049109:Cell Proliferation / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D011829:Radiation Dosage; D015398:Signal Transduction / Q000528:radiation effects*; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1
keywords: Apoptosis; Egr1; Gene-radiotherapy; MCF-7 cell; Truncated AIF