title: Characterization of the porcine synovial fluid proteome and a comparison to the plasma proteome.
abstract: Synovial fluid is present in all joint cavities, and protects the articular cartilage surfaces in large by lubricating the joint, thus reducing friction. Several studies have described changes in the protein composition of synovial fluid in patients with joint disease. However, the protein concentration, content, and synovial fluid volume change dramatically during active joint diseases and inflammation, and the proteome composition of healthy synovial fluid is incompletely characterized. We performed a normative proteomics analysis of porcine synovial fluid, and report data from optimizing proteomic methods to investigate the proteome of healthy porcine synovial fluid (Bennike et al., 2014 [1]). We included an evaluation of different proteolytic sample preparation techniques, and an analysis of posttranslational modifications with a focus on glycosylation. We used pig (Sus Scrofa) as a model organism, as the porcine immune system is highly similar to human and the pig genome is sequenced. Furthermore, porcine model systems are commonly used large animal models to study several human diseases. In addition, we analyzed the proteome of human plasma, and compared the proteomes to the obtained porcine synovial fluid proteome. The proteome of the two body fluids were found highly similar, underlining the detected plasma derived nature of many synovial fluid components. The healthy porcine synovial fluid proteomics data, human rheumatoid arthritis synovial fluid proteomics data used in the method optimization, human plasma proteomics data, and search results, have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD000935.
authors.affiliation: Department of Health Science and Technology, Aalborg University, Fredrik Bajersvej 3B, 9220 Aalborg, Denmark ; Departments of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
authors.forename: Tue Bjerg
authors.initials: TB
authors.lastname: Bennike
authors.affiliation: Departments of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Barnaby
authors.affiliation: Departments of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA.
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.affiliation: Department of Health Science and Technology, Aalborg University, Fredrik Bajersvej 3B, 9220 Aalborg, Denmark.
authors.forename: Allan
authors.initials: A
authors.lastname: Stensballe
keywords: Digestion; Human; Optimization; Pig; Plasma; Porcine; Post-translational modification; Proteomics; Synovial fluid