title: A (2)H magic-angle spinning solid-state NMR characterisation of lipid membranes in intact bacteria.
abstract: This work proposes a new approach to characterize cell membranes in intact cells by (2)H solid-state nuclear magnetic resonance (NMR) in only a few hours using magic-angle spinning (MAS) and spectral moment analysis. The method was first validated on model dipalmitoylphosphatidylcholine (DPPC) membranes, allowing the detection of lipid fluctuations below the main transition temperature. Then the lipid dynamics in Escherichia coli membranes was compared in bacteria grown under different diets. More specifically, deuterated palmitic acid was used to isotopically label the phospholipid acyl chains in bacteria membranes, with or without the presence of protonated oleic acid. Our results showed improved lipid fluidity when bacteria were grown in the presence of oleic acid, which helps preserving the natural fatty acid profile in E. coli membranes. The MAS (2)H solid-state NMR study of membranes combined with spectral moment analysis showed to be a fast method compatible with in vivo bacterial studies, and should also be applicable to other micro-organisms to obtain molecular information on living cells by solid-state NMR.
authors.affiliation: UMR 7099, CNRS - Université Paris Diderot, IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris, France.
authors.forename: Xavier L
authors.initials: XL
authors.lastname: Warnet
authors.affiliation: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montréal, Canada, H3C 3P8.
authors.forename: Marwa
authors.initials: M
authors.lastname: Laadhari
authors.affiliation: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montréal, Canada, H3C 3P8.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Arnold
authors.affiliation: Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montréal, Canada, H3C 3P8.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Marcotte
authors.affiliation: UMR 7099, CNRS - Université Paris Diderot, IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris, France. Electronic address: Dror.Warschawski@ibpc.fr.
authors.forename: Dror E
authors.initials: DE
authors.lastname: Warschawski
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019301:Oleic Acid / Q000737:chemistry* / Q000494:pharmacology; D019308:Palmitic Acid / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D044366:Transition Temperature
chemical_list: D008051:Lipid Bilayers; D015060:1,2-Dipalmitoylphosphatidylcholine; D019301:Oleic Acid; D019308:Palmitic Acid; D003903:Deuterium
keywords: Bacteria; Deuterium NMR; Dynamics; In cell solid-state nuclear magnetic resonance; Lipids