title: Glycopolymers Prepared by Ring-Opening Metathesis Polymerization Followed by Glycoconjugation Using a Triazole-Forming "Click" Reaction.
abstract: We describe a protocol for the preparation of glycopolymers derived from the ring-opening polymerization of a norbornene carboxylic acid derivative. Polymerization is followed by attachment of a linker and subsequent glycoconjugation via a triazole-forming azide-alkyne click reaction. The use of a protected amine-terminating agent allows for the attachment of a probe molecule such as a fluorescein dye. The syntheses of a neutral galactopolymer as well a polyanionic poly-3-O-sulfo-galactopolymer are described.
authors.affiliation: Department of Chemistry, Brown University, Box H, 324 Brook Street, Providence, RI, 02912, USA.
authors.forename: Ronald
authors.initials: R
authors.lastname: Okoth
authors.affiliation: Department of Chemistry, Brown University, Box H, 324 Brook Street, Providence, RI, 02912, USA. abasu@brown.edu.
authors.forename: Amit
authors.initials: A
authors.lastname: Basu
mesh_terms: D057930:Click Chemistry; D006001:Glycoconjugates / Q000138:chemical synthesis*; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis*; D014230:Triazoles / Q000737:chemistry*
chemical_list: D006001:Glycoconjugates; D011108:Polymers; D014230:Triazoles
keywords: Click chemistry; Fluorescent glycoconjugate; Galactose; Glycopolymer; Linker; Propargyl glycoside; Ring-opening metathesis polymerization; Sulfo-galactose; Triazole