title: Flow cytometry in hematological nonmalignant disorders.
abstract: Multiparameter flow cytometry (MFC) has become an integral part of the diagnosis and classification of hematological malignancies. However, several nonmalignant or premalignant disorders may benefit from this technology in hematology laboratories. This review provides information on the normal immunophenotypic characteristics of peripheral blood leukocyte subsets and their modifications in several clinical conditions. The usefulness of MFC and the specific markers that can be investigated in hyperlymphocytosis, infection, hypereosinophilia, paroxysmal nocturnal hemoglobinuria, and large granular lymphocyte disorders is described. Mention is also made of the developments of MFC for analyses of red blood cells or platelets.
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Béné
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Le Bris
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: N
authors.initials: N
authors.lastname: Robillard
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: S
authors.initials: S
authors.lastname: Wuillème
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Fouassier
authors.affiliation: Hematology Biology, University Hospital of Nantes, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Eveillard
mesh_terms: D015415:Biomarkers; D001772:Blood Cell Count / Q000379:methods; D001792:Blood Platelets / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006402:Hematologic Diseases / Q000097:blood* / Q000175:diagnosis*; D019337:Hematologic Neoplasms / Q000097:blood / Q000175:diagnosis; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007962:Leukocytes / Q000378:metabolism; D010641:Phenotype
chemical_list: D015415:Biomarkers
keywords: Flow cytometry; erythrocytes; leukocytes; nonmalignant disorders; peripheral blood