title: Panax Notoginseng Saponins Ameliorates Coxsackievirus B3-Induced Myocarditis by Activating the Cystathionine-γ-Lyase/Hydrogen Sulfide Pathway.
abstract: This study is to determine the therapeutic effects of Panax notoginseng saponins (PNSs) on coxsackievirus B3 (CVB3)-induced myocarditis, and whether cystathionine-γ-lyase (CSE)/hydrogen sulfide (H2S) pathway is involved. Mouse model of myocarditis was induced by CVB3 infection, and the mice were subjected to vehicle (saline) or drug treatments (sodium bisulfide (NaHS), propargylglycine (PAG), or PNSs). The results showed that there were inflammatory cell infiltrations, interstitial edemas, and elevated inflammatory cytokines, in CVB3-induced myocarditis. PAG administration increased, whereas NaHS treatment decreased the severity of the myocarditis. PNS treatment dramatically alleviated these myocardial injuries and decreased the viral messenger RNA (mRNA) expression by the enhanced expression of CSE/H2S pathway. Moreover, the therapeutic effects of PNSs on myocarditis were stronger than those of NaHS. Finally, the effect of PNSs on CSE/H2S pathway and cardiac cell protection were verified in cultured cardiac cells. PNSs may be a promising medication for viral myocarditis therapy.
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Pan
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Yuanhai
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Children's Center, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Lu
authors.affiliation: Children's Center, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Geng
authors.affiliation: Children's Center, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Jia
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Rong
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Zhenquan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China.
authors.forename: Rongzhou
authors.initials: R
authors.lastname: Wu
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China. chmping@126.com.
authors.forename: Maoping
authors.initials: M
authors.lastname: Chu
authors.affiliation: Children's Heart Center, The Second Affiliated Hospital & Yuying Children's Hospital, Institute of cardiovascular development and translational medicine, Wenzhou Medical University, Wenzhou, 325027, China. chunxiang_zhang@rush.edu.|Department of Pharmacology and Cardiovascular Research Center, Rush Medical College of Rush University Medical Center, Chicago, IL, 60612, USA. chunxiang_zhang@rush.edu.
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D003384:Coxsackievirus Infections / Q000188:drug therapy / Q000503:physiopathology; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D004195:Disease Models, Animal; D029822:Enterovirus B, Human / Q000187:drug effects / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000821:virology*; D053723:Panax notoginseng; D008517:Phytotherapy / Q000379:methods; D028321:Plant Preparations / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012503:Saponins / Q000627:therapeutic use; D015398:Signal Transduction
chemical_list: D015415:Biomarkers; D028321:Plant Preparations; D034741:RNA, Small Interfering; D012503:Saponins; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide
keywords: Cystathionine-γ-lyase; Hydrogen sulfide; Myocarditis; Panax notoginseng saponins