title: NEDD4-1 and PTEN expression in keloid scarring.
abstract: Keloid scarring remains a major problem in plastic surgery. The aim of this study was to determine the expression of the PTEN tumor suppressor and NEDD4-1 genes in keloid tissue and explore their effect on the formation of such scarring. Twenty keloid patients were enrolled in the study and underwent surgical removal of keloid tissue. No patient had received chemotherapy and/or radiotherapy prior to treatment. PTEN and NEDD4-1 mRNA expression was detected by reverse transcription PCR, while PTEN protein expression was assessed using immunohistochemistry. Our results showed that levels of PTEN were significantly diminished in keloid samples (P < 0.05), whereas those of NEDD4-1 did not significantly differ between keloid tissue and normal skin (P > 0.05). Furthermore, we found that NEDD4-1 expression is high and inversely correlated with that of PTEN in keloids. Our results suggest that the PTEN/PI3K/AKT pathway may play an important role in keloid formation and reduces PTEN expression in such tissue. Finally, although NEDD4-1 has previously been identified as a factor in keloid susceptibility, and the protein for which it encodes is known to degrade PTEN by catalyzing its polyubiquitylation, the detailed mechanism behind its involvement in keloid formation needs to be further studied.
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: P F
authors.initials: PF
authors.lastname: Sang
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: C
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: J S
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D000075702:Nedd4 Ubiquitin Protein Ligases; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D000075702:Nedd4 Ubiquitin Protein Ligases; C000618483:Nedd4 protein, human; D044767:Ubiquitin-Protein Ligases; D051059:PTEN Phosphohydrolase