title: Water deprivation-partial rehydration induces sensitization of sodium appetite and alteration of hypothalamic transcripts.
abstract: iSodium intake occurs either as a spontaneous or induced behavior, which is enhanced, i.e., sensitized, by repeated episodes of water deprivation followed by subsequent partial rehydration (WD-PR). In the present work, we examined whether repeated WD-PR alters hypothalamic transcripts related to the brain renin-angiotensin system (RAS) and apelin system in male normotensive Holtzman rats (HTZ). We also examined whether the sodium intake of a strain with genetically inherited high expression of the brain RAS, the spontaneously hypertensive rat (SHR), responds differently than HTZ to repeated WD-PR. We found that repeated WD-PR, besides enhancing spontaneous and induced 0.3 M NaCl intake, increased the hypothalamic expression of angiotensinogen, aminopeptidase N, and apelin receptor transcripts (43%, 60%, and 159%, respectively) in HTZ at the end of the third WD-PR. Repeated WD-PR did not change the daily spontaneous 0.3 M NaCl intake and barely changed the need-induced 0.3 M NaCl intake of SHR. The same treatment consistently enhanced spontaneous daily 0.3 M NaCl intake in the normotensive Wistar-Kyoto rats. The results show that repeated WD-PR produces alterations in hypothalamic transcripts and also sensitizes sodium appetite in HTZ. They suggest an association between the components of hypothalamic RAS and the apelin system, with neural and behavioral plasticity produced by repeated episodes of WD-PR in a normotensive strain. The results also indicate that the inherited hyperactive brain RAS is not a guarantee for sensitization of sodium intake in the male adult SHR exposed to repeated WD-PR.
authors.affiliation: Department of Physiology and Pathology, School of Dentistry, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil;
authors.forename: Daniela T B
authors.initials: DT
authors.lastname: Pereira-Derderian
authors.affiliation: Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil; and.
authors.forename: Regina C
authors.initials: RC
authors.lastname: Vendramini
authors.affiliation: Department of Physiology and Pathology, School of Dentistry, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil;
authors.forename: José V
authors.initials: JV
authors.lastname: Menani
authors.affiliation: Department of Pharmacology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Chiavegatto
authors.affiliation: Department of Physiology and Pathology, School of Dentistry, São Paulo State University-UNESP, Araraquara, São Paulo, Brazil; lucajr@foar.unesp.br.
authors.forename: Laurival A
authors.initials: LA
authors.lastname: De Luca
mesh_terms: D000818:Animals; D000073861:Apelin; D001069:Appetite Regulation; D001522:Behavior, Animal; D004195:Disease Models, Animal; D005440:Fluid Therapy; D005786:Gene Expression Regulation; D006973:Hypertension / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology / Q000523:psychology; D007031:Hypothalamus / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009473:Neuronal Plasticity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000235:genetics; D017673:Sodium Chloride, Dietary / Q000008:administration & dosage*; D013997:Time Factors; D014868:Water Deprivation
chemical_list: D000073861:Apelin; C498579:Apln protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary
keywords: angiotensin; apelin; dehydration; neuroplasticity; rat strain; spontaneously hypertensive rat