title: Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1.
abstract: The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20min) and 99.5% (40min) at 850mbar and 25°C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450mbar, 35°C and 40min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8days, respectively.
authors.affiliation: University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chun-Yen
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.lastname: Jesisca
authors.affiliation: Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.
authors.forename: Chienyan
authors.initials: C
authors.lastname: Hsieh
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Chien-Hsiang
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D018533:Biomass; D002708:Chlorella / Q000378:metabolism*; D005663:Furans / Q000737:chemistry; D014975:Lutein / Q000096:biosynthesis* / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D000074242:Sunflower Oil; D013696:Temperature
chemical_list: D005663:Furans; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; D000074242:Sunflower Oil; C018674:tetrahydrofuran; D014975:Lutein
keywords: Chlorella sorokiniana; Lutein; Microalgae; Reduced pressure extraction; Stabilization