title: Blood pH in coronary artery microthrombosis of rats.
abstract: OBJECTIVE
  To study the mechanism and significance of pH change in the coronary artery microthrombosis of rats.
  
  
  METHODS
  After the sodium laurate-induced model of coronary artery microthrombosis of rats was constructed, the vascular endothelial cells were separated and then cultured in the mediums with different pH values for 24 h. Enzyme linked immunosorbent assay was used to detect the content of von Willebrand factor (vWF) in the medium; while the real-time PCR and western blot assay were used to detect the expression of fibrinogen-like protein 2 (FGL2) at the mRNA and protein level. The comprehensive evaluation was performed to discuss the effect of pH change on the coronary artery microthrombosis of rats.
  
  
  RESULTS
  The expression level of vWF detected by enzyme linked immunosorbent assay was 336.67 ± 24.95, 311.33 ± 14.98, 359.67 ± 39.63, 354.67 ± 49.01 and 332.00 ± 33.42 (pg/mL) respectively; while the expression of vWF in the model group was 570.00 ± 57.94, 524.67 ± 57.94, 437.00 ± 95.38, 415.33 ± 44.38 and 444.67 ± 74.31 respectively. Being cultured under the different pH values, the relative expression level of FGL2 mRNA in the model group was 7.93 ± 0.93, 6.70 ± 0.70, 5.03 ± 0.32, 5.13 ± 0.40 and 5.57 ± 0.83 respectively.
  
  
  CONCLUSIONS
  The coronary artery microthrombosis of rats can cause the high expression and secretion of vWF. Meanwhile, FGL2 is also up-regulated in the thrombosis and such up-regulation is more significant in the condition with low pH, which indicates that the low pH condition may be one of factors that contribute to the cardiovascular diseases.
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China; Department of Cardiology, Affiliated Huiyang Hospital of Southern Medical University, Huizhou, Guangdong Province 516200, China; Department of Cardiology, Huizhou No.6 People's Hospital, Huizhou, Guangdong Province 516200, China.
authors.forename: Kuai-Fa
authors.initials: KF
authors.lastname: Fang
authors.affiliation: Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province 510080, China.
authors.forename: Zhu-Jun
authors.initials: ZJ
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China. Electronic address: kuaifa@gmail.com.
authors.forename: Ping-Sheng
authors.initials: PS
authors.lastname: Wu
authors.affiliation: Postdoctoral Research Station, Ji'nan Military General Hospital, Ji'nan 250000, China; The PLA 401 Hospital, Qingdao 266071, China.
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Yu
keywords: Coronary artery; FGL2; Microthrombus; pH