title: An apolipoprotein B100 mimotope prevents obesity in mice.
abstract: Although apolipoprotein B100 (ApoB100) plays a key role in peripheral fat deposition, it is not considered a suitable therapeutic target in obesity. In the present study we describe a novel ApoB100 mimotope, peptide pB1, and the use of pB1-based vaccine-like formulations (BVFs) against high-fat diet (HFD)-induced obesity. In HFD- compared with chow-fed adolescent mice, BVFs reduced the 3-month body-weight gains attributable to increased dietary fat by 44-65%, and prevented mesenteric fat accumulation and liver steatosis. The body-weight reductions paralleled the titres of pB1-reactive immunoglobulin G (IgG) antibodies, and pB1-reactive antibodies specifically recognized native ApoB100 and a synthetic peptide from the C-terminal half of ApoB100. In cultured 3T3L1 adipocytes, anti-pB1 antibodies increased lipolysis and inhibited low-density lipoprotein (LDL) uptake. In cultured RAW 264.7 macrophages, the same antibodies enhanced LDL uptake (without causing foam cell formation). These findings make ApoB100 a promising target for an immunization strategy against HFD-induced obesity.
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea SJ Biomed Inc., HBI 604, 55 Hanyangdaehak-ro, Ansan, 426-791, Republic of Korea kimhj104@hanyang.ac.kr bbinas@hanyang.ac.kr.
authors.forename: Hyo Joon
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Hee Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Jung Soon
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Jemin
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Hyun Kyun
authors.initials: HK
authors.lastname: Na
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea.
authors.forename: Hae-Jung
authors.initials: HJ
authors.lastname: Joung
authors.affiliation: Pathology, Korea University Medical School, Ansan Hospital, 123 Jeokgeum-ro, Danwon-gu, Ansan, 425-707, Republic of Korea.
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Molecular & Life Science, College of Science & Technology, Hanyang University (ERICA), Ansan, 426-791, Republic of Korea kimhj104@hanyang.ac.kr bbinas@hanyang.ac.kr.
authors.forename: Bert
authors.initials: B
authors.lastname: Binas
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000276:immunology / Q000627:therapeutic use*; D000917:Antibody Formation / Q000187:drug effects; D053299:Apolipoprotein B-100 / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D059305:Diet, High-Fat / Q000009:adverse effects; D000939:Epitopes / Q000276:immunology; D005234:Fatty Liver / Q000517:prevention & control; D000960:Hypolipidemic Agents / Q000276:immunology / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D009765:Obesity / Q000517:prevention & control*; D010455:Peptides / Q000276:immunology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019440:Anti-Obesity Agents; D053299:Apolipoprotein B-100; D000939:Epitopes; D000960:Hypolipidemic Agents; D008077:Lipoproteins, LDL; D010455:Peptides; C000606726:pB1 peptide
keywords: ApoB100; high-fat-diet-induced obesity; humoral immunity; mimotope