title: Synthesis, Characterization and Fluorescence Properties of Zn(II) and Cu(II) Complexes: DNA Binding Study of Zn(II) Complex.
abstract: Zinc(II) and copper(II) complexes containing Schiff base, 2- methoxy-6((E)-(phenylimino) methyl) phenol ligand (HL) were synthesized and characterized by elemental analysis, IR, NMR, and single crystal X-ray diffraction technique. The fluorescence properties and quantum yield of zinc complex were studied. Our data showed that Zn complex could bind to DNA grooves with Kb = 10(4) M(-1). Moreover, Zn complex could successfully be used in staining of DNA following agarose gel electrophoresis. MTT assay showed that Zn complex was not cytotoxic in MCF-7 cell line. Here, we introduce a newly synthesized fluorescence probe that can be used for single and double stranded DNA detection in both solution and agarose gels.
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran.
authors.forename: Parirokh
authors.initials: P
authors.lastname: Lavaee
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran. heshtiagh@um.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Eshtiagh-Hosseini
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Housaindokht
authors.affiliation: Department of Chemistry, Tulane University, New Orleans, LA, 70118, USA.
authors.forename: Joel T
authors.initials: JT
authors.lastname: Mague
authors.affiliation: Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, 917751436, Iran.
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Esmaeili
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Khalil
authors.initials: K
authors.lastname: Abnous
mesh_terms: D001665:Binding Sites; D003300:Copper / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D003300:Copper; D004247:DNA; D015032:Zinc
keywords: Copper; DNA; Fluorescence; Interaction; Schiff base; Zinc