title: [CXCR3 monoclonal antibody inhibits the proliferation and migration of MCF-7 cells and HepG2 cells in vitro].
abstract: OBJECTIVE
  To study the effects of chemokine (C-X-C motif) receptor 3 (CXCR3) monoclonal antibody (mAb) on the proliferation and migration of MCF-7 and HepG2 cells in vitro.
  
  
  METHODS
  Ascites of CXCR3 mAb was prepared at first. MCF-7 and HepG2 cells with high expressions of CXCR3 were screened by flow cytometry. MTT assay was used to detect the effects of CXCR3 mAb on the proliferation of MCF-7 and HepG2 cells in vitro in the absence/presence of interferon-inducible T-cell alpha chemoattractant (I-TAC). Transwell™ assay was performed to investigate the effects of CXCR3 mAb on the migration of MCF-7 and HepG2 cells in vitro in the absence/presence of I-TAC.
  
  
  RESULTS
  The expression rate of CXCR3 on MCF-7 and HepG2 cells were 83.5% and 96.2%, respectively. 50 mg/mL CXCR3 mAb significantly inhibited the proliferation and migration of MCF-7 and HepG2 cells, and also inhibited the promoting effect of I-TAC on the proliferation and migration of MCF-7 and HepG2 cells in vitro.
  
  
  CONCLUSIONS
  CXCR3 mAb can significantly inhibit the proliferation and migration of the tumor cells highly expressing CXCR3 in vitro.
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Zhiyao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Department of Immunology, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Qiu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002465:Cell Movement; D049109:Cell Proliferation; D054371:Chemokine CXCL11 / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D054367:Receptors, CXCR3 / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; C515202:CXCL11 protein, human; C515187:CXCR3 protein, human; D054371:Chemokine CXCL11; D054367:Receptors, CXCR3