title: Assessing Mitochondrial Functions in Candida albicans.
abstract: This chapter is designed to present methods for characterizing the annotation of genes associated with mitochondrial functions in Candida spp. Methods include drop plate assays for evaluating inhibitors of the respiratory electron transport system complexes as well as measuring the enzyme activity of complex I-V enzyme activities. Assays are also presented to measure toxic ROS production that accompanies gene mutations or gene loss and chronological aging that often is shortened in Complex I dysfunction. Also presented are methods to isolate mitochondria, visualize mitochondria, and extract mitochondrial proteins.
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC, 20057, USA.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC, 20057, USA. calderor@georgetown.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Calderone
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D045222:Electron Transport Chain Complex Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species
chemical_list: D045222:Electron Transport Chain Complex Proteins; D017382:Reactive Oxygen Species
keywords: Complexes; Electron transport chain; Gel electrophoresis; Isolation; Life span; Mitochondria