title: Gene expression profiling of bone marrow-derived stromal cells seeded onto a sandblasted, large-grit, acid-etched-treated titanium implant surface: The role of the Wnt pathway.
abstract: OBJECTIVE
  The physical and chemical characteristics of the titanium implant surface have been shown to influence dental implant fixation. However, the underlying mechanism by which sandblasted, large-grit, acid-etched (SLA) treatment affects osseointegration remains elusive.
  
  
  METHODS
  In the present study, the involved target genes and pathways for SLA treatment, which is an extensively used implant surface modification on improving osseointegration, were identified by in vitro microarray and bioinformatics analyses.
  
  
  RESULTS
  A total of 19 genes were differentially expressed after SLA treatment, which included Apc2, Fzd1, Frzb, Wnt16, Fzd2, Plau, Wnt5b, Wnt5a, Lrp6, Wnt9a, Sfrp4, Prkch, Calcoco1, Ccnd1, Wif1, Fzd4, Myc, LRP5, and Lect2. Interaction pathway analyses showed that the Wnt pathway was the most relevant signal after SLA treatment. To ensure the reliability of microarray data, LRP5 was shown to positively regulate osteogenic commitment, extracellular matrix synthesis, and mineralization for BMMSCs seeded onto an SLA-treated titanium surface. However, with LRP5 shRNA treatment, the reduction in calcium deposition in the SLA-treated group was more severe than that observed in cells seeded onto SLA-untreated titanium surface, suggesting that the function of LRP5 was reinforced in the SLA-treated group. In addition, the present study demonstrated that the β-catenin/LRP5 pathway was responsible for the enhanced osteogenic responses of BMMSCs on SLA-treated titanium surface.
  
  
  CONCLUSIONS
  The findings of the present study serve as an initial step towards understanding the mechanism underlying SLA treatment in osseointegration.
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Yang
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Fang
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Tie
authors.initials: T
authors.lastname: Liu
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Fuming
authors.initials: F
authors.lastname: He
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Hospital of Stomatology, Medical College, Zhejiang University, Yan'an Road 395, Hangzhou 310000, People's Republic of China. Electronic address: gsunrise@aliyun.com.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Guan
mesh_terms: D000134:Acid Etching, Dental; D000818:Animals; D002478:Cells, Cultured; D019295:Computational Biology; D045662:Dental Etching / Q000379:methods*; D015921:Dental Implants; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D046228:Microarray Analysis; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013057:Spectrum Analysis; D013499:Surface Properties; D014025:Titanium; D060449:Wnt Signaling Pathway / Q000235:genetics*
chemical_list: D015921:Dental Implants; D014025:Titanium
keywords: LRP5; Microarray; Osseointegration; SLA treatment; Wnt pathway; β-Catenin