title: A nanoaggregate-on-mirror platform for molecular and biomolecular detection by surface-enhanced Raman spectroscopy.
abstract: A nanoaggregate-on-mirror (NAOM) structure has been developed for molecular and biomolecular detection using surface-enhanced Raman spectroscopy (SERS). The smooth surface of the gold mirror allows for simple and homogeneous functionalization, while the introduction of the nanoaggregates enhances the Raman signal of the molecule(s) in the vicinity of the aggregate-mirror junction. This is evidenced by functionalizing the gold mirror with 4-nitrothiophenol, and the further addition of gold nanoaggregates promotes local SERS activity only in the areas with the nanoaggregates. The application of the NAOM platform for biomolecular detection is highlighted using glucose and H2O2 as molecules of interest. In both cases, the gold mirror is functionalized with 4-mercaptophenylboronic acid (4-MPBA). Upon exposure to glucose, the boronic acid moiety of 4-MPBA forms a cyclic boronate ester. Once the nanoaggregates are added to the surface, detection of glucose is possible without the use of an enzyme. This method of indirect detection provides a limit of detection of 0.05 mM, along with a linear range of detection from 0.1 to 15 mM for glucose, encompassing the physiological range of blood glucose concentration. The detection of H2O2 is achieved with optical inspection and SERS. The H2O2 interferes with the coating of the gold mirror, enabling qualitative detection by visual inspection. Simultaneously, the H2O2 reacts with the boronic acid to form a phenol, a change that is detected by SERS.
authors.affiliation: Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.|Centre for Advanced Materials and Biomaterials Research, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
authors.forename: Gregory Q
authors.initials: GQ
authors.lastname: Wallace
authors.affiliation: Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.|Centre for Advanced Materials and Biomaterials Research, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
authors.forename: Mohammadali
authors.initials: M
authors.lastname: Tabatabaei
authors.affiliation: Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.|Centre for Advanced Materials and Biomaterials Research, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
authors.forename: Mariachiara S
authors.initials: MS
authors.lastname: Zuin
authors.affiliation: Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.|Centre for Advanced Materials and Biomaterials Research, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Workentin
authors.affiliation: Department of Chemistry, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada. flagugne@uwo.ca.|Centre for Advanced Materials and Biomaterials Research, University of Western Ontario, 1151 Richmond St., London, Ontario, N6A 5B7, Canada. flagugne@uwo.ca.
authors.forename: François
authors.initials: F
authors.lastname: Lagugné-Labarthet
mesh_terms: D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D010636:Phenols; D013438:Sulfhydryl Compounds; D006046:Gold; C042983:thiophenol; D006861:Hydrogen Peroxide; D005947:Glucose
keywords: 4-Mercaptophenylboronic acid; Glucose; Hydrogen peroxide; Nanoaggregate-on-mirror; Surface-enhanced Raman spectroscopy