title: Antioxidant Activity and Validation of Quantification Method for Lycopene Extracted from Tomato.
abstract: Lycopene is a carotenoid found in tomatoes with potent antioxidant activity. The aim of the study was to obtain an extract containing lycopene from four types of tomatoes, validate a quantification method for the extracts by HPLC, and assess its antioxidant activity. Results revealed that the tomatoes analyzed contained lycopene and antioxidant activity. Salad tomato presented the highest concentration of this carotenoid and antioxidant activity. The quantification method exhibited linearity with a correlation coefficient of 0.9992. Tests for the assessment of precision, accuracy, and robustness achieved coefficients with variation of less than 5%. The LOD and LOQ were 0.0012 and 0.0039 μg/mL, respectively. Salad tomato can be used as a source of lycopene for the development of topical formulations, and based on performed tests, the chosen method for the identification and quantification of lycopene was considered to be linear, precise, exact, selective, and robust.
authors.affiliation: University of São Paulo State, Faculty of Pharmaceutical Sciences, Araraquara-Jau Highway, Km 1, Araraquara, São Paulo, Brazil.
authors.forename: Letícia Caramori
authors.initials: LC
authors.lastname: Cefali
authors.forename: Edith Cristina Laignier
authors.initials: EC
authors.lastname: Cazedey
authors.forename: Tatiana Maria
authors.initials: TM
authors.lastname: Souza-Moreira
authors.forename: Marcos Antônio
authors.initials: MA
authors.lastname: Correa
authors.forename: Hérida Regina Nunes
authors.initials: HR
authors.lastname: Salgado
authors.forename: Vera Lucia Borges
authors.initials: VL
authors.lastname: Isaac
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002138:Calibration; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D059625:Liquid-Liquid Extraction / Q000379:methods; D000077276:Lycopene; D018551:Solanum lycopersicum / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000975:Antioxidants; D002338:Carotenoids; D000077276:Lycopene