title: Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil.
abstract: This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiabá (n = 3), Várzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Belgath Fernandes
authors.initials: BF
authors.lastname: Cardoso
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Otacília Pereira
authors.initials: OP
authors.lastname: Serra
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Letícia Borges da Silva
authors.initials: LB
authors.lastname: Heinen
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Nayara
authors.initials: N
authors.lastname: Zuchi
authors.affiliation: Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz, Manaus, AM, Brasil.
authors.forename: Victor Costa de
authors.initials: VC
authors.lastname: Souza
authors.affiliation: Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz, Manaus, AM, Brasil.
authors.forename: Felipe Gomes
authors.initials: FG
authors.lastname: Naveca
authors.affiliation: Laboratório Central, Secretaria de Estado de Saúde de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Marcelo Adriano Mendes dos
authors.initials: MA
authors.lastname: Santos
authors.affiliation: Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, MT, Brasil.
authors.forename: Renata Dezengrini
authors.initials: RD
authors.lastname: Slhessarenko
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D063147:Animal Distribution; D000818:Animals; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002044:Bunyaviridae Infections / Q000097:blood / Q000453:epidemiology*; D002522:Chlorocebus aethiops; D003465:Culex / Q000145:classification / Q000821:virology*; D003715:Dengue / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D005334:Fever / Q000503:physiopathology / Q000821:virology; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016851:Orthobunyavirus / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012367:RNA, Viral / Q000302:isolation & purification*; D065288:Serogroup; D012840:Simbu virus / Q000145:classification* / Q000235:genetics; D014709:Vero Cells; D055815:Young Adult
chemical_list: D012367:RNA, Viral