title: IL-10 regulate decidual Tregs apoptosis contributing to the abnormal pregnancy with Toxoplasma gondii infection.
abstract: This study aims to investigate whether IL-10 regulate decidual Treg cells apoptosis to reverse the abnormal pregnancy outcomes with Toxoplasma gondii (T. gondii) infection. Recombinant mouse IL-10 (rIL-10) treatment and IL-10 deficiency (IL-10(-/-)) abnormal pregnancy animal models with T. gondii infection were established. Apoptosis related molecules cleaved Caspase-3 and Caspase-8 in decidual Treg cells were examined using flow cytometry. The levels of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were up-regulated with T. gondii infection. Compared to infected group, the expressions of cleaved Caspase-3 and Caspase-8 in decidual Treg cells were down-regulated in rIL-10-treated group, while up-regulated in infected IL-10(-/-) group. In addition, pregnant outcomes were improved in rIL-10-treated group, while worse in IL-10(-/-) group compared to infected group. These findings revealed that IL-10 reduced the decidual Treg cells apoptosis contributing to improving adverse pregnant outcomes following T. gondii infection.
authors.affiliation: Department of Gynecology and Obstetrics, Binzhou Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong, 256603, PR China.
authors.forename: Kaixue
authors.initials: K
authors.lastname: Lao
authors.affiliation: Department of Radiology, Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong, 256603, PR China.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China.
authors.forename: Zhidan
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China.
authors.forename: Xianbing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Gynecology and Obstetrics, Binzhou Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong, 256603, PR China. Electronic address: docylwang2003@126.com.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Binzhou Medical University, Yantai, Shandong, 264003, PR China. Electronic address: xue-mei-hu@163.com.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D003656:Decidua / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007155:Immunologic Factors / Q000008:administration & dosage / Q000378:metabolism; D016753:Interleukin-10 / Q000008:administration & dosage / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology*; D011251:Pregnancy Complications, Infectious / Q000276:immunology* / Q000473:pathology; D011256:Pregnancy Outcome; D059748:Proteolysis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D014123:Toxoplasmosis / Q000276:immunology* / Q000473:pathology
chemical_list: D007155:Immunologic Factors; D016753:Interleukin-10; C505526:Casp8 protein, mouse; D053148:Caspase 3; D053181:Caspase 8
keywords: Abnormal pregnancy outcomes; Apoptosis; IL-10; Regulatory T cells; Toxoplasma gondii