title: The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains.
abstract: Considering the importance of macrophages as the first line of defense against fungal infection and the different roles played by the two M1- and M2-like polarized macrophages, we decided to evaluate the effects of Paracoccidioides brasiliensis infection on GM-CSF- and M-CSF-induced bone marrow-derived macrophages (BMM) from the A/J and B10.A mouse strains, an established model of resistance/susceptibility to PCM, respectively. Upon differentiation, the generated GM- or M-BMMs were characterized by morphological analyses, gene expression profiles, and cytokines production. Our main results demonstrate that GM-BMMs derived from A/J and B.10 produced high levels of pro- and anti-inflammatory cytokines that may contribute to generate an unbalanced early immune response. In accordance with the literature, the B10.A susceptible mice lineage has an innate tendency to polarize into M1-like phenotype, whereas the opposite phenotype occurs in A/J resistance mice. In this context, our data support that susceptibility and resistance are strongly correlated with M1 and M2 polarization, respectively.
authors.affiliation: Laboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, Brazil.
authors.forename: Calliandra
authors.initials: C
authors.lastname: de Souza Silva
authors.affiliation: Laboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, Brazil.
authors.forename: Aldo Henrique
authors.initials: AH
authors.lastname: Tavares
authors.affiliation: Laboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, Brazil.
authors.forename: Marcio
authors.initials: M
authors.lastname: Sousa Jeronimo
authors.affiliation: Laboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, Brazil.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Soares de Lima
authors.affiliation: Laboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, Brazil.
authors.forename: Lorena
authors.initials: L
authors.lastname: da Silveira Derengowski
authors.affiliation: Laboratório de Imunologia Aplicada, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Campus Universitário Darcy Ribeiro, 70910-900 Brasília, DF, Brazil.
authors.forename: Anamélia Lorenzetti
authors.initials: AL
authors.lastname: Bocca
authors.affiliation: Laboratório de Biologia Molecular, CEL/IB, Campus Darcy Ribeiro, Asa Norte, 70910-900 Brasília, DF, Brazil.
authors.forename: Ildinete
authors.initials: I
authors.lastname: Silva-Pereira
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D007249:Inflammation; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D010228:Paracoccidioides; D010229:Paracoccidioidomycosis / Q000378:metabolism*; D010587:Phagocytosis; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D018925:Chemokines; D016207:Cytokines; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor