title: Characterization of a cfr-Carrying Plasmid from Porcine Escherichia coli That Closely Resembles Plasmid pEA3 from the Plant Pathogen Erwinia amylovora.
abstract: The multiresistance gene cfr was found in two porcine Escherichia coli isolates, one harboring it on the conjugative 33,885-bp plasmid pFSEC-01, the other harboring it in the chromosomal DNA. Sequence analysis of pFSEC-01 revealed that a 6,769-bp fragment containing the cfr gene bracketed by two IS26 elements was inserted into a plasmid closely related to pEA3 from the plant pathogen Erwinia amylovora, suggesting that pFSEC-01 may be transferred between different bacterial genera of both animal and plant origin.
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Rongmin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Animal Science and Veterinary Medicine College of Heilongjiang Bayi Agriculture University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Lei
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany wucm@cau.edu.cn stefan.schwarz@fli.bund.de.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
authors.affiliation: Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China wucm@cau.edu.cn stefan.schwarz@fli.bund.de.
authors.forename: Congming
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D044084:Erwinia amylovora / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D016366:Open Reading Frames; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C534716:Cfr protein, E coli; D008780:Methyltransferases