title: [Mechanism Underlying Increased Expression of a Member of the Serine/Threonine Kinase Family (Citron kinase) Induced by HIV-1 Infection].
abstract: Human immunodeficiency virus (HIV)-1 infection changes transcriptional profiles and regulates. the factors and machinery of the host that facilitate viral replication. Our previous study suggested that the serine/threonine kinase citron kinase (citK) promotes HIV-1 egress. To ascertain if HIV-1 infection affects citK expression in primary cells, peripheral blood mononuclear cells were infected with vesicular stomatitis virus G protein (VSV-G)-pseudotyped HIV-1 vector NL4-3-luc viruses, which resulted in remarkably increased expression of citK. citK overexpression led to a more than two-fold increase in HIV-1 production, whereas a significant decrease was observed when citK was depleted in CD4+ T cells. Infection with HIV-1 pseudoviruses induced increases in the mRNA and protein levels of citK by 2. 5- and 2. 7-fold in HEK293T cells, respectively. By cloning the 5-kb promoter of citK into a luciferase reporter system and transfecting the construct into HEK293T cells, enhanced luciferase activity was observed during HIV-1 infection. Taken together, these data demonstrate that HIV-1 infection upregulates citK expression at the transcriptional level, and thereby renders the host more susceptible to invasion by HIV-1.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Ding
authors.forename: Zeyun
authors.initials: Z
authors.lastname: Mi
authors.forename: Jianyuan
authors.initials: J
authors.lastname: Zhao
authors.forename: Jinming
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D057809:HEK293 Cells; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015854:Up-Regulation; D014779:Virus Replication
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C115635:citron-kinase; D017346:Protein Serine-Threonine Kinases