title: Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells.
abstract: Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of γH2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. marina.poettler@uk-erlangen.de.
authors.forename: Marina
authors.initials: M
authors.lastname: Pöttler
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. andreas.staicu@uk-erlangen.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Staicu
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. jan.zaloga@uk-erlangen.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Zaloga
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. harald.unterweger@uk-erlangen.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Unterweger
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. bianca.weigel@uk-erlangen.de.
authors.forename: Bianca
authors.initials: B
authors.lastname: Weigel
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. eveline.schreiber@uk-erlangen.de.
authors.forename: Eveline
authors.initials: E
authors.lastname: Schreiber
authors.affiliation: Department of Obstetrics and Gynecology, Ludwig Maximilians University of Munich, Maistraße 11, Munich 80337, Germany. simone.hofmann@med.uni-muenchen.de.
authors.forename: Simone
authors.initials: S
authors.lastname: Hofmann
authors.affiliation: Department of Obstetrics and Gynecology, Ludwig Maximilians University of Munich, Maistraße 11, Munich 80337, Germany. irmgard.wiest@med.uni-muenchen.de.
authors.forename: Irmi
authors.initials: I
authors.lastname: Wiest
authors.affiliation: Department of Obstetrics and Gynecology, Ludwig Maximilians University of Munich, Maistraße 11, Munich 80337, Germany. udo.jeschke@med.uni-muenchen.de.
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. christoph.alexiou@uk-erlangen.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Alexiou
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung Professorship, University Hospital Erlangen, Glückstraße 10a, Erlangen 91054, Germany. christina.janko@uk-erlangen.de.
authors.forename: Christina
authors.initials: C
authors.lastname: Janko
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D003911:Dextrans / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005290:Ferric Compounds / Q000378:metabolism / Q000633:toxicity*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D058185:Magnetite Nanoparticles / Q000633:toxicity*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D009152:Mutagenicity Tests
chemical_list: D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; C065507:ferumoxides
keywords: cancer therapy and diagnosis; granulosa cells; protein corona; reproductive health; superparamagnetic iron oxide nanoparticles