title: Non-genomic regulation and disruption of spermatozoal in vitro hyperactivation by oviductal hormones.
abstract: During capacitation, motility of mammalian spermatozoon is changed from a state of "activation" to "hyperactivation." Recently, it has been suggested that some hormones present in the oviduct are involved in the regulation of this hyperactivation in vitro. Progesterone, melatonin, and serotonin enhance hyperactivation through specific membrane receptors, and 17β-estradiol suppresses this enhancement by progesterone and melatonin via a membrane estrogen receptor. Moreover, γ-aminobutyric acid suppresses progesterone-enhanced hyperactivation through the γ-aminobutyric acid receptor. These hormones dose-dependently affect hyperactivation. Although the complete signaling pathway is not clear, progesterone activates phospholipase C and protein kinases and enhances tyrosine phosphorylation. Moreover, tyrosine phosphorylation is suppressed by 17β-estradiol. This regulation of spermatozoal hyperactivation by steroids is also disrupted by diethylstilbestrol. The in vitro experiments reviewed here suggest that mammalian spermatozoa are able to respond to effects of oviductal hormones. We therefore assume that the enhancement of spermatozoal hyperactivation is also regulated by oviductal hormones in vivo.
authors.affiliation: Department of Physiology, School of Medicine, Dokkyo Medical University, Mibu, Tochigi, 321-0293, Japan. fujinoki@dokkyomed.ac.jp.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Fujinoki
authors.affiliation: Department of Physiology, School of Medicine, Dokkyo Medical University, Mibu, Tochigi, 321-0293, Japan.
authors.forename: Gen L
authors.initials: GL
authors.lastname: Takei
authors.affiliation: Laboratory Animal Research Center, School of Medicine, Dokkyo Medical University, Mibu, Tochigi, 321-0293, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Kon
mesh_terms: D000818:Animals; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000502:physiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008550:Melatonin / Q000502:physiology*; D010057:Oviducts / Q000502:physiology*; D011374:Progesterone / Q000502:physiology*; D012701:Serotonin / Q000502:physiology; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
chemical_list: D012701:Serotonin; D011374:Progesterone; D004958:Estradiol; D004054:Diethylstilbestrol; D008550:Melatonin
keywords: Amine; Amino acid; Hyperactivation; Non-genomic regulation; Spermatozoa; Steroid