title: [Protective effects of polydatin on HK-2 cells against oxygen-glucose deprivation/re-oxygenation-induced injury by regulating Sonic hedgehog through PI3K/Akt signaling pathway].
abstract: OBJECTIVE
  To explore the effects of polydatin on human kidney tubular epithelial cells (HK-2 cells) with oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced injury and potential mechanisms.
  
  
  METHODS
  HK-2 cells were cultured under normal or OGD/R condition with different drug treatment methods, including 10, 20 and 40 μmol/L polydatin, and 1 μmol/L Wortmannin, a specific phosphatidylinositol 3-kinase (PI3K) inhibitor. MTT assay was used to detect the survival ability of cells in different groups. The contents of tumor necrosis factor α (TNF-α) and interleukine 1β (IL-1β) in supernatant fluids of the cultured cells were examined by ELISA. And Western blotting was performed to determine the protein levels of total Akt (t-Akt), phospho-Akt (p-Akt) and Sonic hedgehog (Shh) in different groups.
  
  
  RESULTS
  Polydatin significantly improved the viability of cells with OGD/R treatment, and apparently inhibited the secretion of TNF-α and IL-1β induced by OGD/R. The inhibition of PI3K/Akt signaling pathway counteracted the anti-inflammation and pro-survival effects of polydatin and blocked the protein expression of Shh in HK-2 cells. The exogenous addition of human recombinant Shh protein not only improved the survival of cells with OGD/R treatment, but also inhibited the inflammation induced by OGD/R in HK-2 cells.
  
  
  CONCLUSIONS
  Polydatin can exert protective effects on HK-2 cells with OGD/R through regulating the PI3K/Akt-dependent Shh pathway.
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Hongbao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Natural Medicine, School of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wang
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D005960:Glucosides / Q000494:pharmacology*; D053823:Hedgehog Proteins / Q000502:physiology*; D006801:Humans; D000860:Hypoxia / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D005960:Glucosides; D053823:Hedgehog Proteins; C509239:SHH protein, human; D013267:Stilbenes; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C058229:polydatin