title: Antisense inhibition of apolipoprotein (a) to lower plasma lipoprotein (a) levels in humans.
abstract: Epidemiological, genetic association, and Mendelian randomization studies have provided strong evidence that lipoprotein (a) [Lp(a)] is an independent causal risk factor for CVD, including myocardial infarction, stroke, peripheral arterial disease, and calcific aortic valve stenosis. Lp(a) levels >50 mg/dl are highly prevalent (20% of the general population) and are overrepresented in patients with CVD and aortic stenosis. These data support the notion that Lp(a) should be a target of therapy for CVD event reduction and to reduce progression of aortic stenosis. However, effective therapies to specifically reduce plasma Lp(a) levels are lacking. Recent animal and human studies have shown that Lp(a) can be specifically targeted with second generation antisense oligonucleotides (ASOs) that inhibit apo(a) mRNA translation. In apo(a) transgenic mice, an apo(a) ASO reduced plasma apo(a)/Lp(a) levels and their associated oxidized phospholipid (OxPL) levels by 86 and 93%, respectively. In cynomolgus monkeys, a second generation apo(a) ASO, ISIS-APO(a)Rx, significantly reduced hepatic apo(a) mRNA expression and plasma Lp(a) levels by >80%. Finally, in a phase I study in normal volunteers, ISIS-APO(a)Rx ASO reduced Lp(a) levels and their associated OxPL levels up to 89 and 93%, respectively, with minimal effects on other lipoproteins. ISIS-APO(a)Rx represents the first specific and potent drug in clinical development to lower Lp(a) levels and may be beneficial in reducing CVD events and progression of calcific aortic valve stenosis.
authors.affiliation: Isis Pharmaceuticals University of California San Diego, La Jolla, CA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Graham
authors.affiliation: Isis Pharmaceuticals University of California San Diego, La Jolla, CA.
authors.forename: Nick
authors.initials: N
authors.lastname: Viney
authors.affiliation: Isis Pharmaceuticals University of California San Diego, La Jolla, CA.
authors.forename: Rosanne M
authors.initials: RM
authors.lastname: Crooke
authors.affiliation: Isis Pharmaceuticals University of California San Diego, La Jolla, CA Division of Cardiovascular Medicine, University of California San Diego, La Jolla, CA stsimikas@ucsd.edu.
authors.forename: Sotirios
authors.initials: S
authors.lastname: Tsimikas
mesh_terms: D000818:Animals; D001054:Apolipoproteins A / Q000172:deficiency* / Q000235:genetics*; D002986:Clinical Trials as Topic; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood*; D016376:Oligonucleotides, Antisense / Q000235:genetics*
chemical_list: D001054:Apolipoproteins A; D017270:Lipoprotein(a); D016376:Oligonucleotides, Antisense
keywords: aortic stenosis; atherosclerosis; myocardial infarction