title: JNK1 inhibits transcriptional and pro-apoptotic activity of TAp63γ.
abstract: TAp63γ is a homologue of tumor suppressor p53 and functions as a transcriptional factor playing key roles in cell cycle and cell apoptosis. In the present work, we find that JNK1 can physically interact with N-terminal transactivation domain (TAD) of TAp63. Overexpression of JNK1 inhibits TAp63γ-mediated transcription, while knockdown or inhibition of endogenous JNK1 increases transactivity of TAp63γ. Further study reveals that Ser12 site in TAD is critical for JNK1-mediated inhibition of TAp63γ. This JNK1-mediated inhibition can impair pro-apoptotic activity of TAp63γ. Together, we report a novel regulation of TAp63γ transactivity and pro-apoptotic activity mediated by JNK1.
authors.affiliation: Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Chengdu 610065, China; Department of Medical Oncology, The Seventh People's Hospital of Chengdu, Chengdu 610041, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Endodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 400015, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Medical Oncology, The Seventh People's Hospital of Chengdu, Chengdu 610041, China.
authors.forename: Dingyi
authors.initials: D
authors.lastname: Liu
authors.affiliation: Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Chengdu 610065, China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Center of Growth, Metabolism and Aging, Key Laboratory of Biological Resources and Ecological Environment of Ministry of Education, College of Life Sciences, Chengdu 610065, China. Electronic address: lichenghua@scu.edu.cn.
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
mesh_terms: D017209:Apoptosis; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D048055:Mitogen-Activated Protein Kinase 8 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D048055:Mitogen-Activated Protein Kinase 8
keywords: Apoptosis; TAp63γ; Transactivity; c-Jun N-terminal kinase 1