title: pH and redox sensitive albumin hydrogel: A self-derived biomaterial.
abstract: Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0-10.0) values and the gelation begins at a concentration of 150 μM and 4.5-8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of in vivo biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on in vitro drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery.
authors.affiliation: CSIR-CLRI Adyar, Chennai, Tamil Nadu, India.
authors.forename: S Thirupathi Kumara
authors.initials: ST
authors.lastname: Raja
authors.affiliation: CSIR-CLRI Adyar, Chennai, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Thiruselvi
authors.affiliation: CSIR-CLRI Adyar, Chennai, Tamil Nadu, India.
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
authors.affiliation: CSIR-CLRI Adyar, Chennai, Tamil Nadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Gnanamani
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D010084:Oxidation-Reduction; D017208:Rats, Wistar; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013752:Tetracycline / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D012709:Serum Albumin; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012710:Serum Albumin, Bovine; D013752:Tetracycline