title: Improvement of Protein Immobilization and Bioactivity of Magnetic Carriers Using a Brushed Beads-on-Beads Structure.
abstract: To achieve higher protein immobilization and bioactivity, as well as automatic manipulation, we prepared a new type of biocarrier based on the brushed beads-on-beads structure. Many poly(acrylic acid) (PAA) brushed nanoparticles were packed onto the surface of amino-functionalized magnetic particles through an efficient carbodiimide-assisted coupling reaction to attain a hierarchical structure, a unique three-dimensional (3D) space and automatic manipulation characteristics. The proposed biocarrier was evaluated in the recognition capability of the immunocomplex and showed a 6.7-fold increase compared with control beads with a hard surface. The results of this study suggest promising applications in targeted capture and high-performance biodetection processes.
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Peirui
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University , Shanghai 200030, P. R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D001667:Binding, Competitive; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D002234:Carbodiimides / Q000737:chemistry; D004337:Drug Carriers; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006514:Hepatitis B Surface Antigens / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D008280:Magnetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D002234:Carbodiimides; D004337:Drug Carriers; D004800:Enzymes, Immobilized; D006514:Hepatitis B Surface Antigens; D058185:Magnetite Nanoparticles; D011108:Polymers; C433673:polyetherimide; C006903:carbopol 940; D001710:Biotin; D006735:Horseradish Peroxidase
keywords: automatic manipulation; brushed beads-on-beads; fluidlike interface; higher protein immobilization; immunocomplex; recognition capability