title: Transcriptomics Identify CD9 as a Marker of Murine IL-10-Competent Regulatory B Cells.
abstract: Regulatory B cells (Breg) have immune suppressive functions in various autoimmune/inflammation models and diseases and are found to be enriched in diverse B cell subsets. The lack of a unique marker or set of markers efficiently identifying Breg cells impedes detailed investigation into their origin, development, and immunological roles. Here, we perform transcriptome analysis of IL-10-expressing B cells to identify key regulators for Breg biogenesis and function and identify CD9, a tetraspanin-family transmembrane protein, as a key surface marker for most mouse IL-10(+) B cells and their progenitors. CD9 plays a role in the suppressive function of IL-10(+) B cells in ex vivo T cell proliferation assays through a mechanism that is dependent upon B/T cell interactions. CD9(+) B cells also demonstrate inhibition of Th1-mediated contact hypersensitivity in an in vivo model system. Taken together, our findings implicate CD9 in the immunosuppressive activity of regulatory B cells.
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Systems Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Jiguang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Pefanis
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Jaime
authors.initials: J
authors.lastname: Chao
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Gerson
authors.initials: G
authors.lastname: Rothschild
authors.affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Tachibana
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Jun Kui
authors.initials: JK
authors.lastname: Chen
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Ivaylo I
authors.initials: II
authors.lastname: Ivanov
authors.affiliation: Department of Systems Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Raul
authors.initials: R
authors.lastname: Rabadan
authors.affiliation: Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: Department of Microbiology and Immunology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. Electronic address: ub2121@cumc.columbia.edu.
authors.forename: Uttiya
authors.initials: U
authors.lastname: Basu
mesh_terms: D000818:Animals; D060151:B-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D060245:Tetraspanin 29 / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D060245:Tetraspanin 29; D016753:Interleukin-10