title: HLA-G 14 bp insertion/deletion polymorphism and its association with sHLA-G levels in Behçet's disease Tunisian patients.
abstract: The purpose of this study was to investigate the HLA-G 3'UTR 14 bp polymorphism and sHLA-G levels in Tunisian patients with BD. The study included 119 patients with BD and 170 healthy blood donors (HD). HLA-G 14 bp polymorphism was genotyped by polymerase chain reaction. Serum levels of soluble HLA-G (sHLA-G) were measured using a commercial ELISA kit. A significant increased frequency of the -14 bp HLA-G allele was detected in patients with BD compared to HD (0.58 vs 0.49, p=0.023), and a significant increased frequency of HLA-G -14/-14 bp was observed in patients with BD compared to HD [0.37 vs 0.22, p=0.007, OR 2.04 (95% CI 1.21-3.42)]. The mean plasmatic concentration of sHLA-G levels were significantly increased in patients with active disease [231.63±286.4 U/mL] compared to those with inactive disease (103.14±77.8 U/mL, p=0.03) and HD (121.41±24.1 U/mL, p=0.04). Furthermore, our results showed that there is no association between HLA-G 14 bp polymorphism and sHLA-G plasma levels.
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia.
authors.forename: K
authors.initials: K
authors.lastname: Sakly
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Maatouk
authors.affiliation: Department of Internal Medicine, F.B. University Hospital, Monastir, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Hammami
authors.affiliation: Department of Internal Medicine, F.B. University Hospital, Monastir, Tunisia.
authors.forename: O
authors.initials: O
authors.lastname: Harzallah
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia.
authors.forename: W
authors.initials: W
authors.lastname: Sakly
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Feki
authors.affiliation: UMR, Paris Diderot, Paris 7 University, Lariboisiere Hospital, INSERM U965, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Mirshahi
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Ghedira
authors.affiliation: Faculty of Pharmacy, Research Unit 03/UR/07 "Autoimmunity and Allergy", University of Monastir, Tunisia; Laboratory of Microbiology, Unit of Immunology of EPS Fattouma Bourguiba, Monastir, Tunisia. Electronic address: saklynabil@yahoo.fr.
authors.forename: N
authors.initials: N
authors.lastname: Sakly
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000293:Adolescent; D000328:Adult; D001528:Behcet Syndrome / Q000235:genetics / Q000276:immunology*; D002648:Child; D004252:DNA Mutational Analysis; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059951:HLA-G Antigens / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional / Q000235:genetics; D011110:Polymorphism, Genetic; D014416:Tunisia; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D059951:HLA-G Antigens
keywords: Behçet’s disease; Genetic polymorphism; HLA-G antigens; Tunisia