title: A Rapid, Selective and Sensitive UPLC-MS/MS Method for Quantification of Nomilin in Rat Plasma and Its Application in a Pharmacokinetic Study.
abstract: Nomilin is a potential anticancer agent. In this study, a rapid, sensitive, and simple ultra-performance liquid chromatography with tandem mass spectrometry methodology was established and validated to quantify nomilin in rat plasma. Plasma samples were prepared through liquid-liquid extraction using ethyl acetate. Chromatographic separation was performed using an Acquity HSS T3 column. Acetonitrile and water containing 0.1% (v/v) formic acid were used as mobile phases at a flow rate of 0.3 mL/min. Nomilin and quercetin (internal standard) were detected and quantified via a triple quadrupole tandem mass spectrometer in the positive ion mode with multiple reaction monitoring. Tandem mass spectrometry detection was performed by monitoring the fragmentations of m/z 515.3 → m/z 161.0 and m/z 303.2 → m/z 153.1 of nomilin and quercetin, respectively. Good linearity (R(2) > 0.996) was observed in the concentration range of 1 ng/mL to 500 ng/mL with a lower limit of quantification of 1 ng/mL for nomilin. The average extraction recoveries of nomilin and quercetin were > 82.3% and 82.0%, respectively. Intra- and interday precisions were less than 15% and accuracy ranged from 85.0% to 90.1%. Indeed, the proposed method was successfully applied to analyze the pharmacokinetics of nomilin after 3 and 50 mg/kg nomilin were administered to rats via intravenous and oral routes, respectively.
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yuepiao
authors.initials: Y
authors.lastname: Cai
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Hu
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
mesh_terms: D000818:Animals; D001584:Benzoxepins / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036701:Limonins / Q000097:blood* / Q000493:pharmacokinetics; D059625:Liquid-Liquid Extraction; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001584:Benzoxepins; D036701:Limonins; C059405:nomilin