title: Molecular Cloning and Characterization of DXS and DXR Genes in the Terpenoid Biosynthetic Pathway of Tripterygium wilfordii.
abstract: 1-Deoxy-d-xylulose-5-phosphate synthase (DXS) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR) genes are the key enzyme genes of terpenoid biosynthesis but still unknown in Tripterygium wilfordii Hook. f. Here, three full-length cDNA encoding DXS1, DXS2 and DXR were cloned from suspension cells of T. wilfordii with ORF sizes of 2154 bp (TwDXS1, GenBank accession no.KM879187), 2148 bp (TwDXS2, GenBank accession no.KM879186), 1410 bp (TwDXR, GenBank accession no.KM879185). And, the TwDXS1, TwDXS2 and TwDXR were characterized by color complementation in lycopene accumulating strains of Escherichia coli, which indicated that they encoded functional proteins and promoted lycopene pathway flux. TwDXS1 and TwDXS2 are constitutively expressed in the roots, stems and leaves and the expression level showed an order of roots > stems > leaves. After the suspension cells were induced by methyl jasmonate, the mRNA expression level of TwDXS1, TwDXS2, and TwDXR increased, and triptophenolide was rapidly accumulated to 149.52 µg·g(-1), a 5.88-fold increase compared with the control. So the TwDXS1, TwDXS2, and TwDXR could be important genes involved in terpenoid biosynthesis in Tripterygium wilfordii Hook. f.
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. tongyuru13@126.com.
authors.forename: Yuru
authors.initials: Y
authors.lastname: Tong
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. suping120@163.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Su
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. diana1989119@sina.com.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. zhangmeng8846@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. tmwangxj@ccmu.edu.cn.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. lyjlife@sina.com.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. xnzhang@ccmu.edu.cn.
authors.forename: Xianan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China. weigao@ccmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. huangluqi01@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014166:Transferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029757:Tripterygium / Q000201:enzymology / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; D014166:Transferases; C109461:deoxyxylulose-5-phosphate synthase; D019747:Aldose-Ketose Isomerases
keywords: Tripterygium wilfordii; TwDXR; TwDXS1; TwDXS2; color complementation