title: Cytotoxicity assessment of graphene-based nanomaterials on human dental follicle stem cells.
abstract: Graphene-oxide (GO) and its most encountered derivatives, thermally reduced graphene oxide (TRGO) and nitrogen-doped graphene (N-Gr), were synthesized and structurally characterized by spectroscopic techniques, like Raman and (13)C MAS solid state NMR. Several biological effects (cytotoxicity, oxidative stress induction, and cellular and mithocondrial membrane alterations) induced by such graphene-based materials on human dental follicle stem cells were investigated. Graphene oxide shows the lowest cytotoxic effect, followed by the nitrogen-doped graphene, while thermally reduced graphene oxide exhibits high cytotoxic effects. Graphene oxide induces oxidative stress without causing cell membrane damage. Nitrogen-doped graphene shows a slight antioxidant activity; however, at high doses (20 and 40 μg/ml) it causes membrane damage. Both graphene oxide and nitrogen-doped graphene seem to be valuable candidates for usage in dental nanocomposites.
authors.affiliation: Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Olteanu
authors.affiliation: Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania. Electronic address: adrianafilip33@yahoo.com.
authors.forename: Adriana
authors.initials: A
authors.lastname: Filip
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Crina
authors.initials: C
authors.lastname: Socaci
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Alexandru Radu
authors.initials: AR
authors.lastname: Biris
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Xenia
authors.initials: X
authors.lastname: Filip
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Maria
authors.initials: M
authors.lastname: Coros
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Marcela Corina
authors.initials: MC
authors.lastname: Rosu
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania.
authors.forename: Florina
authors.initials: F
authors.lastname: Pogacean
authors.affiliation: Department of Propedeutics and Dental Materials, Faculty of Dental Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 32, Cluj-Napoca, Romania.
authors.forename: Camelia
authors.initials: C
authors.lastname: Alb
authors.affiliation: Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, Clinicilor 1-3, Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Baldea
authors.affiliation: University of Agricultural Sciences and Veterinary Medicine, Department of Pathology, Calea Manastur 3-5, 400372 Cluj-Napoca, Romania; Department of Biomedical Sciences, Ross University School of Veterinary Medicine Basseterre, St. Kitts, West Indies.
authors.forename: Pompei
authors.initials: P
authors.lastname: Bolfa
authors.affiliation: National Institute for Research and Development of Isotopic and Molecular Technologies, Donat Street No. 67-103, Cluj-Napoca 400293, Romania. Electronic address: stela.pruneanu@itim-cj.ro.
authors.forename: Stela
authors.initials: S
authors.lastname: Pruneanu
mesh_terms: D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007223:Infant; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D014070:Tooth / Q000166:cytology / Q000187:drug effects*
chemical_list: D006108:Graphite
keywords: Cytotoxicity assessment; Graphene based nanomaterials; Human dental follicle stem cells