title: Liver receptor homologue-1 and steroidogenic factor-1 expression in cultured granulosa cells from patients with endometriosis: A preliminary study.
abstract: OBJECTIVE
  The expression of aromatase (via CYP19 and the CYP19 PII promoter) and the orphan nuclear receptor family members, liver receptor homologue-1 (LRH-1) and steroidogenic factor-1 (SF-1) in cultured luteinized granulosa cells from women with endometriosis were investigated.
  
  
  METHODS
  Luteinized granulosa cells from patients undergoing in vitro fertilization (16 patients with endometriosis and 28 controls) were examined for messenger ribonucleic acid (mRNA) expression of CYP19, CYP19 PII, LRH-1 and SF-1, determined by quantitative reverse transcription-polymerase chain reaction. Student's t-test, the Mann-Whitney U test and Spearman's rank correlation were used for statistical analysis.
  
  
  RESULTS
  The number of high quality embryos in the endometriosis group was significantly lower than in the control group. The mRNA expression levels of CYP19, CYP19 PII, LRH-1 and SF-1 in granulosa-lutein cells were decreased in women with endometriosis compared to the control group. The simultaneous down-regulation expression of LRH-1, SF-1 and CYP19 PII in endometriotic granulosa cells indicated their positive correlation.
  
  
  CONCLUSIONS
  Our results demonstrate aberrant expressions of SF-1 and LRH-1 in endometriotic granulosa-lutein cells. This finding may be helpful in understanding infertility associated with endometriosis and reduced P450 aromatase activity in endometriotic granulosa cells.
authors.affiliation: Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Zheng-mu
authors.initials: ZM
authors.lastname: Wu
authors.affiliation: Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Yong-wei
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Cheng
mesh_terms: D000328:Adult; D001141:Aromatase / Q000235:genetics; D002478:Cells, Cultured; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D054339:Steroidogenic Factor 1 / Q000235:genetics*
chemical_list: C082631:NR5A2 protein, human; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D001141:Aromatase; C561410:CYP19A1 protein, human
keywords: CYP19 gene; endometriosis; granulosa cells; liver receptor homologue-1; steroidogenic factor-1