title: High quality draft genomes of the Mycoplasma mycoides subsp. mycoides challenge strains Afadé and B237.
abstract: Members of the Mycoplasma mycoides cluster' represent important livestock pathogens worldwide. Mycoplasma mycoides subsp. mycoides is the etiologic agent of contagious bovine pleuropneumonia (CBPP), which is still endemic in many parts of Africa. We report the genome sequences and annotation of two frequently used challenge strains of Mycoplasma mycoides subsp. mycoides, Afadé and B237. The information provided will enable downstream 'omics' applications such as proteomics, transcriptomics and reverse vaccinology approaches. Despite the absence of Mycoplasma pneumoniae like cyto-adhesion encoding genes, the two strains showed the presence of protrusions. This phenotype is likely encoded by another set of genes.
authors.affiliation: International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya ; International Centre for Insect Physiology and Ecology, PO Box 30772, 00100 Nairobi, Kenya.
authors.forename: Anne
authors.initials: A
authors.lastname: Fischer
authors.affiliation: Institute for Genome Sciences, University of Maryland School of Medicine, 801 W. Baltimore Street BioPark II, 21201 Baltimore, MD USA.
authors.forename: Ivette
authors.initials: I
authors.lastname: Santana-Cruz
authors.affiliation: Institute of Functional and Applied Anatomy, Hannover Medical School, Hannover, Germany ; Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL), Hannover, Germany ; REBIRTH Cluster of Excellence, Hannover, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Hegerman
authors.affiliation: Department of Animal Breeding and Genetics, SLU Global Bioinformatics Centre, Swedish University of Agricultural Sciences, SE75007 Uppsala, Sweden.
authors.forename: Hadrien
authors.initials: H
authors.lastname: Gourlé
authors.affiliation: International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya.
authors.forename: Elise
authors.initials: E
authors.lastname: Schieck
authors.affiliation: Department of Animal Breeding and Genetics, SLU Global Bioinformatics Centre, Swedish University of Agricultural Sciences, SE75007 Uppsala, Sweden.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Lambert
authors.affiliation: Institute for Genome Sciences, University of Maryland School of Medicine, 801 W. Baltimore Street BioPark II, 21201 Baltimore, MD USA.
authors.forename: Suvarna
authors.initials: S
authors.lastname: Nadendla
authors.affiliation: Kenya Agricultural and Livestock Research Organization (KALRO) Muguga, PO Box 32-00902, Kikuyu, Kenya.
authors.forename: Hezron
authors.initials: H
authors.lastname: Wesonga
authors.affiliation: International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya ; Department of Food Science, Cornell University, Ithaca, NY USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Miller
authors.affiliation: J. Craig Venter Institute, 9704 Medical Center Drive, 20850 Rockville, MD USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Vashee
authors.affiliation: Lausanne Genomic Technologies Facility Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Johann
authors.initials: J
authors.lastname: Weber
authors.affiliation: Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Meens
authors.affiliation: Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland.
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.affiliation: International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya ; Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, Switzerland.
authors.forename: Joerg
authors.initials: J
authors.lastname: Jores
keywords: Challenge strain; Contagious bovine pleuropneumonia; Genome; Mycoplasma mycoides subsp. mycoides; Protrusion