title: Synthesis of functional polyester for fabrication of nano-fibrous scaffolds and its effect on PC12 cells.
abstract: An ideal scaffold should mimic the advantageous characteristics of a natural extracellular matrix for cell attachment, proliferation, and differentiation. In this study, well-defined block copolymer with functional groups was synthesized. The structure of the block copolymer was characterized by nuclear magnetic resonance, gel permeation chromatography, and differential scanning calorimetry. Thermally induced phase separation was employed to fabricate nano-fibrous scaffolds based on the synthesized block copolymer. The scaffold, with fiber diameter ranging from 400 to 500 nm, was fabricated for in vitro culture of PC12 cells. The carboxyl groups on the side chain resulted in increased hydrophilicity of nano-fibrous scaffolds and enhanced cell proliferation. In addition, this scaffold structure was beneficial in directing the growth of regenerating axons in nerve tissue engineering. Results of 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scanning electron microscopy confirmed that the nano-fibrous scaffolds with functional groups were suitable for PC12 cells growth. Moreover, the carboxyl groups were suitable for coupling with biological signals. Thus, the nano-fibrous scaffolds have potential applications in tissue engineering.
authors.affiliation: a Department of Chemical Engineering , Huizhou University , Huizhou , China.
authors.forename: Na
authors.initials: N
authors.lastname: Qiang
authors.affiliation: b Department of Orthopaedics , West China Hospital, Sichuan University , Chengdu , China.|c Department of Orthopaedics , Mianyang Center Hospital , Mianyang , China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.affiliation: b Department of Orthopaedics , West China Hospital, Sichuan University , Chengdu , China.
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Shi
authors.affiliation: a Department of Chemical Engineering , Huizhou University , Huizhou , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: a Department of Chemical Engineering , Huizhou University , Huizhou , China.
authors.forename: Yi-hong
authors.initials: YH
authors.lastname: Ma
authors.affiliation: a Department of Chemical Engineering , Huizhou University , Huizhou , China.
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Tao
authors.affiliation: d Department of Orthopaedics , Guangdong Hospital of Traditional Chinese Medicine , Guangzhou , China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D018929:Cell Culture Techniques; D049108:Cell Enlargement; D049109:Cell Proliferation; D002850:Chromatography, Gel; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry; D009416:Nerve Regeneration; D016501:Neurites; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D016716:PC12 Cells; D044367:Phase Transition; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters
keywords: Block copolymer; PC12 cells; functional groups; nano-fibrous scaffolds