title: Genomic characterization of Ralstonia solanacearum phage ϕRS138 of the family Siphoviridae.
abstract: ϕRS138, a bacteriophage of the family Siphoviridae that lyses Ralstonia solanacearum, was isolated. The genomic DNA of ϕRS138 was 41,941 bp long with a GC content of 65.1 % and contained 56 putative open reading frames. The ϕRS138 genome could be divided into three regions based on similarities to other genomes: (1) a region containing genes encoding a putative transcriptional regulator and an integrase, similar to the prophage genes in Ralstonia solanacearum K60-1; (2) a region encoding proteins related to structural modules and virion morphogenesis, similar to genes in the Pseudomonas phages of the family Siphoviridae; and (3) a region highly similar to the genomes of other Ralstonia solanacearum strains.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.|Department of Molecular Biotechnology, Biotechnology Research and Development Institute, Can Tho University, Cantho City, Vietnam.
authors.forename: Bich
authors.initials: B
authors.lastname: Van Truong Thi
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
authors.forename: Nguyen Huan
authors.initials: NH
authors.lastname: Pham Khanh
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Namikawa
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
authors.forename: Kaito
authors.initials: K
authors.lastname: Miki
authors.affiliation: Hiyoshi Corporation, 908 Kitanosho, Omihachiman, Shiga, 523-8555, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kondo
authors.affiliation: Department of Molecular and Environmental Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam.
authors.forename: Phuong Thao
authors.initials: PT
authors.lastname: Dang Thi
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kame@kit.ac.jp.
authors.forename: Kaeko
authors.identifier: http://orcid.org/0000-0002-7496-4692
authors.initials: K
authors.lastname: Kamei
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D043368:Ralstonia solanacearum / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D017906:Siphoviridae / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral