title: [Diagnostic performance of graphical anomalies in the detection of large platelets and platelet clumps].
abstract: Thrombocytopenia is a current situation for making a blood smear in routine practice in a medical analysis laboratory. Recent automated hematology analyzers enumerate platelets and generate histograms and specific flags. Operators must be aware of the characteristics of their analyzer in order to avoid spurious results in the case where microscopy review is not possible.
  
  
  OBJECTIVE
  We evaluated the diagnostic performance of various graphical anomalies in the detection of large platelets and platelet clumps.
  
  
  METHODS
  Three hundred cases of thrombocytopenia were included in the study on the basis of a platelet count less than 150 × 10(9)/L. This evaluation is expressed by the results of the sensitivity, specificity, positive predictive value and negative predictive value compared to the microscopic review of blood smear.
  
  
  RESULTS
  Graphical performances are variable according to microscopic review of blood smears. Indeed, a not fitted curve is the most sensitive change on platelet histogram to the presence of large platelet. A high specificity to the presence of platelet clumps is announced when the platelet curve fails to return to the baseline. Moreover, characteristic findings on the DIFF scattergram are very specific to the presence of platelet clumps.
  
  
  CONCLUSIONS
  A normal platelet histogram can validate with great confidence thrombocytopenia in cases where a blood smear cannot be read immediately.
authors.affiliation: Laboratoire du centre de maternité et de néonatologie de Monastir, 5000 Monastir, Tunisie; Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie. Electronic address: mouna.bahrini@yahoo.fr.
authors.forename: M
authors.initials: M
authors.lastname: Sassi
authors.affiliation: Laboratoire du centre de maternité et de néonatologie de Monastir, 5000 Monastir, Tunisie; Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie.
authors.forename: W
authors.initials: W
authors.lastname: Dibej
authors.affiliation: Laboratoire du centre de maternité et de néonatologie de Monastir, 5000 Monastir, Tunisie; Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie.
authors.forename: B
authors.initials: B
authors.lastname: Abdi
authors.affiliation: Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie; Laboratoire d'hématologie et banque du sang, hôpital Fattouma Bourguiba, 5000, Monastir, Tunisie.
authors.forename: F
authors.initials: F
authors.lastname: Abderrazak
authors.affiliation: Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie; Laboratoire d'hématologie et banque du sang, hôpital Fattouma Bourguiba, 5000, Monastir, Tunisie.
authors.forename: M
authors.initials: M
authors.lastname: Hassine
authors.affiliation: Laboratoire du centre de maternité et de néonatologie de Monastir, 5000 Monastir, Tunisie; Faculté de pharmacie, université de Monastir, 5000 Monastir, Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Babba
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000473:pathology; D048429:Cell Size; D003581:Cytodiagnosis / Q000379:methods / Q000592:standards; D006801:Humans; D010363:Pattern Recognition, Automated / Q000592:standards; D010974:Platelet Aggregation; D010976:Platelet Count / Q000295:instrumentation / Q000379:methods; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013921:Thrombocytopenia / Q000097:blood / Q000175:diagnosis*
keywords: Agrégats plaquettaires; Blood smear; Frottis sanguin; Histogram; Histogramme; Large platelets; Macroplaquettes; Platelet clumps; Thrombocytopenia; Thrombopénie