title: Sialomes and Mialomes: A Systems-Biology View of Tick Tissues and Tick-Host Interactions.
abstract: Tick saliva facilitates tick feeding and infection of the host. Gene expression analysis of tick salivary glands and other tissues involved in host-pathogen interactions has revealed a wide range of bioactive tick proteins. Transcriptomic analysis has been a milestone in the field and has recently been enhanced by next-generation sequencing (NGS). Furthermore, the application of quantitative proteomics to ticks with unknown genomes has provided deeper insights into the molecular mechanisms underlying tick hematophagy, pathogen transmission, and tick-host-pathogen interactions. We review current knowledge on the transcriptomics and proteomics of tick tissues from a systems-biology perspective and discuss future challenges in the field.
authors.affiliation: Faculty of Science, University of South Bohemia in České Budějovice, Budweis, Czech Republic; Department of Clinical Pathobiochemistry, Technische Universität Dresden, Dresden, Germany.
authors.forename: Jindřich
authors.initials: J
authors.lastname: Chmelař
authors.affiliation: Faculty of Science, University of South Bohemia in České Budějovice, Budweis, Czech Republic; Institute of Parasitology, Biology Center, Czech Academy of Sciences, Budweis, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kotál
authors.affiliation: Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS, USA.
authors.forename: Shahid
authors.initials: S
authors.lastname: Karim
authors.affiliation: Institute of Parasitology, Biology Center, Czech Academy of Sciences, Budweis, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Kopacek
authors.affiliation: Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA.
authors.forename: Ivo M B
authors.initials: IMB
authors.lastname: Francischetti
authors.affiliation: Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
authors.forename: Joao H F
authors.initials: JHF
authors.lastname: Pedra
authors.affiliation: Institute of Parasitology, Biology Center, Czech Academy of Sciences, Budweis, Czech Republic. Electronic address: mich_kotsyfakis@yahoo.com.
authors.forename: Michail
authors.initials: M
authors.lastname: Kotsyfakis
mesh_terms: D000818:Animals; D006790:Host-Parasite Interactions / Q000235:genetics / Q000502:physiology*; D006801:Humans; D020543:Proteome; D049490:Systems Biology; D013987:Ticks / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D020543:Proteome
keywords: next-generation sequencing; sialomes; systems biology; tick-borne pathogens