title: Apolipoprotein A1 -75 G/A and +83 C/T polymorphisms and renal cancer risk.
abstract: BACKGROUND
  Apolipoprotein A1 (ApoA1) is the major apoprotein constituent of high-density lipoprotein that can play important roles in tumor invasion and metastasis. The objective of the present study was to evaluate the association of two genetic variants (-75 G/A and +83 C/T) of APOA1 with predisposition to renal cancer.
  
  
  METHODS
  A total of 432 subjects, including 216 pathologically-proven renal cancer cases and 216 age- and gender-matched healthy controls, were recruited into this hospital-based case-control study. Genotyping of the APOA1 was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with gel electrophoresis, and then confirmed by direct sequencing.
  
  
  RESULTS
  Patients with renal cancer had a significantly higher frequency of APOA1 -75 AA genotype [odds ratio (OR) = 2.10, 95% confidence interval (CI) = 1.18, 3.75; P = 0.01] and APOA1 -75 A allele (OR =1.40, 95% CI = 1.05, 1.87; P = 0.02) than controls. When stratifying by the distant metastasis status, patients with distant metastasis had a significantly higher frequency of APOA1 -75 AA genotype genotype (OR =2.20, 95% CI = 1.04, 4.68; P = 0.04).
  
  
  CONCLUSIONS
  This study is, to our knowledge, the first to examine prospectively an increased risk role of APOA1 -75 AA genotype and APOA1 -75 A allele in renal cancer susceptibility.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: ZhiHong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Urology, The Second people's Hospital of Sichuan, Chengdu, 610041, China.
authors.forename: YingMing
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: LiangYou
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: YuJie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: RuoChen
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Department of Urology, West China Hospital, Sichuan University, No 37, Guo Xue Xiang, Chengdu, 610041, China. ypinglu@outlook.com.
authors.forename: YiPing
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I