title: From Structure to Function: A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand Their Role in Cytokinesis.
abstract: Unveiling the function of a novel protein is a challenging task that requires careful experimental design. Yeast cytokinesis is a conserved process that involves modular structural and regulatory proteins. For such proteins, an important step is to identify their domains and structural organization. Here we briefly discuss a collection of methods commonly used for sequence alignment and prediction of protein structure that represent powerful tools for the identification homologous domains and design of structure-function approaches to test experimentally the function of multi-domain proteins such as those implicated in yeast cytokinesis.
authors.affiliation: Centre de Recherche, Institut Curie, 75248, Paris, France.|CNRS UMR144, 75248, Paris, France.
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Rincon
authors.affiliation: Centre de Recherche, Institut Curie, 75248, Paris, France. anne.paoletti@curie.fr.|CNRS UMR144, 75248, Paris, France. anne.paoletti@curie.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Paoletti
mesh_terms: D000595:Amino Acid Sequence; D019295:Computational Biology / Q000379:methods*; D048749:Cytokinesis; D015870:Gene Expression; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D021281:Quantitative Structure-Activity Relationship; D016415:Sequence Alignment; D015003:Yeasts / Q000235:genetics
chemical_list: D011506:Proteins
keywords: Anillin; Cell division; Cytokinesis; Sad kinase; Structure-function analysis