title: Bead milling for lipid recovery from thraustochytrid cells and selective hydrolysis of Schizochytrium DT3 oil using lipase.
abstract: Marine microalgae present a renewable alternative source for sustainable production of omega-3 fatty acids, as compared to conventional sources such as krill oil and fish oil. In this study, we optimised a method for lipid extraction from marine thraustochytrids using a bead mill and enzymatic concentration of omega-3 fatty acids from the thraustochytrid oil. The optimised lipid extraction conditions were, bead size 0.4-0.6μm, 4500rpm, 4min of processing time at 5g biomass concentration. The maximum lipid yield (% dry weight basis) achieved at optimum conditions were 40.5% for Schizochytrium sp. S31 (ATCC) and 49.4% for Schizochytrium sp. DT3 (in-house isolate). DT3 oil contained 39.8% docosahexaenoic acid (DHA) as a percentage of lipid, a higher DHA percentage than S31. Partial hydrolysis of DT3 oil using Candida rugosa lipase was performed to enrich omega-3 polyunsaturated fatty acids (PUFAs) in the glyceride portion. Total omega-3 fatty acid content was increased to 88.7%.
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Avinesh R
authors.initials: AR
authors.lastname: Byreddy
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Waurn Ponds, Geelong, Victoria 3217, Australia. Electronic address: munish.puri@deakin.edu.au.
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
mesh_terms: D000818:Animals; D018533:Biomass; D002175:Candida / Q000378:metabolism; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004281:Docosahexaenoic Acids / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000737:chemistry; D005395:Fish Oils; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry*; D058086:Microalgae; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D004281:Docosahexaenoic Acids; D008049:Lipase
keywords: Bioactives; Candida rugosa; Cell disruption; Enzymes; Schizochytrium