title: [Assessment of Vitamin D Metabolism by LC-MS/MS].
abstract: Vitamin D is pro-hormone that has important roles in calcium metabolism in the intestine, kidneys, and bone. Many studies have indicated that vitamin D deficiency causes not only rickets and osteomalacia, but also increases the risk of various diseases such as cancer and autoimmune disease. Of the many vitamin D metabolites, 25-hydroxyvitamin D[25OH-D], 1α,25-dihydroxyvitamin D[1,25(OH)2-D], and 24,25-dihydroxyvitamin D [24,25 (OH) 2-D] are important for assessing vitamin D metabolism. They are circulating, biologically active, and major inactive forms of vitamin D metabolite, respectively. Immunoassays are widely used for the measurement of serum/plasma vitamin D metabolites. With such an assay, however, the accurate quantification of these metabolites is difficult because of cross-reactivity. Liquid chromatography-tandem mass spectrometry [LC-MS/MS] is the gold standard method for analyzing these metabolites due to its high sensitivity and selectivity. Derivatization with a Cookson-type reagent is a key technique for robust and sensitive analysis by LC-MS/MS. A Cookson-type reagent rapidly and quantitatively reacts with the s-cis-diene structure of vitamin D metabolites, and markedly enhances the ionization efficiency. Using a recently developed Cookson-type reagent (DAPTAD), we successfully established an LC-MS/MS-based method for analyzing serum vitamin D metabolites including 250H-D, 1,25 (OH) 2-D, and 24,25 (OH) 2-D. Thus, LC-MS/MS can be a powerful tool for the accurate determination of vitamin D metabolism. In this review, we describe the advantages of LC-MS/MS for the determination of vitamin D metabolites.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ishige
authors.forename: Mamoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Shoujiro
authors.initials: S
authors.lastname: Ogawa
authors.forename: Motoi
authors.initials: M
authors.lastname: Nishimura
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Higashi
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
mesh_terms: D015650:24,25-Dihydroxyvitamin D 3 / Q000097:blood; D002117:Calcitriol / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014807:Vitamin D / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism*
chemical_list: D011933:Reagent Kits, Diagnostic; D014807:Vitamin D; D015650:24,25-Dihydroxyvitamin D 3; C104450:25-hydroxyvitamin D; D002117:Calcitriol