title: [Identification of medicinal plant Dendrobium based on the chloroplast psbK-psbI intergenic spacer].
abstract: In this paper, the chloroplast psbK-psbI intergenic spacers of 18 species of Dendrobium and their adulterants were amplified and sequenced, and then the sequence characteristics were analyzed. The sequence lengths of chloroplast psbK-psbI regions of Dendrobium ranged from 474 to 513 bp and the GC contents were 25.4%-27.6%. The variable sites were 71 while the informative sites were 46. The inter-specific genetic distances calculated by Kimura 2-parameter (K2P) of Dendrobium were 0.006 1-0.058 1, with an average of 0.028 4. The K2P genetic distances between Dendrobium species and Bulbophyllum odoratissimum were 0.093 2-0.120 4. The NJ tree showed that the Dendrobium species can be easily differentiated from each other and 6 samples of the inspected Dendrobium species were identified successfully through sequencing the psbK-psbI intergenic spacer. Therefore, the chloroplast psbK-psbI intergenic spacer can be used as a candidate marker to identify Dendrobium species and its adulterants.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.forename: Shuang-jiao
authors.initials: SJ
authors.lastname: Ma
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Song
authors.forename: Shi-lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D002736:Chloroplasts; D018742:DNA, Chloroplast / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D031667:Dendrobium / Q000145:classification* / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification* / Q000235:genetics
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer