title: Implementation of automated testing for 1,25-dihydroxyvitamin D: Return of experience from a core-laboratory.
abstract: Measurement of 1,25(OH)2D, the most biologically active form of vitamin D, circulating levels is relevant in several physiopathological states such as chronic kidney disease, parathyroid dysfunction, sarcoidosis, and vitamin D dependent rickets. Our study determined the performances of a novel automated 1,25(OH)2D immunoassay in a core-laboratory environment. We observed satisfactory analytical performances for this assay and an excellent agreement with a well established LC-MS/MS method. Furthermore, this assay allows a reduced TAT, integration in automated core-laboratories and potential consolidation with other tests of the bone and mineral metabolism.
authors.affiliation: Department of Laboratory Medicine, Cliniques Universitaires St-Luc, Brussels, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Miller
authors.affiliation: Department of Laboratory Medicine, Cliniques Universitaires St-Luc, Brussels, Belgium; Pôle de recherche en Endocrinologie, Diabète et Nutrition, Institut de Recherche Expérimentale et Clinique, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Brussels, Belgium. Electronic address: damien.gruson@uclouvain.be.
authors.forename: Damien
authors.initials: D
authors.lastname: Gruson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D057205:Automation, Laboratory / Q000295:instrumentation* / Q000592:standards; D001842:Bone and Bones / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay; D007753:Laboratories; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D014807:Vitamin D; C097949:1,25-dihydroxyvitamin D
keywords: Automation; Bone; Calcitriol; Kidney; Mass spectrometry; Vitamin D