title: Fertilization of Mouse Gametes in Vitro Using a Digital Microfluidic System.
abstract: We demonstrated in vitro fertilization (IVF) using a digital microfluidic (DMF) system, so-called electrowetting on dielectric (EWOD). The DMF device was proved to be biocompatible and the DMF manipulation of a droplet was harmless to the embryos. This DMF platform was then used for the fertilization of mouse gametes in vitro and for embryo dynamic culture based on a dispersed droplet form. Development of the embryos was instantaneously recorded by a time-lapse microscope in an incubator. Our results indicated that increasing the number of sperms for IVF would raise the rate of fertilization. However, the excess of sperms in the 10 μL culture medium would more easily make the embryo dead during cell culture. Dynamic culture powered with EWOD can manipulate a single droplet containing mouse embryos and culture to the eight-cell stage. The fertilization rate of IVF demonstrated by DMF system was 34.8%, and about 25% inseminated embryos dynamically cultured on a DMF chip developed into an eight-cell stage. The results indicate that the DMF system has the potential for application in assisted reproductive technology.
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Huang
authors.forename: Hsien-Hua
authors.initials: HH
authors.lastname: Shen
authors.forename: Lung-Yuan
authors.initials: LY
authors.lastname: Chung
authors.forename: Yu-Hsiang
authors.initials: YH
authors.lastname: Chung
authors.forename: Chih-Chen
authors.initials: CC
authors.lastname: Chen
authors.forename: Chia-Hsien
authors.initials: CH
authors.lastname: Hsu
authors.forename: Shih-Kang
authors.initials: SK
authors.lastname: Fan
authors.forename: Da-Jeng
authors.initials: DJ
authors.lastname: Yao
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D058874:Electrowetting / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation* / Q000379:methods; D008297:Male; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009865:Oocytes / Q000166:cytology; D013094:Spermatozoa / Q000166:cytology