title: Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals.
abstract: Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an anti-bacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Republic of Korea; Hanyang Biomedical Research Institute, Hanyang University, Republic of Korea.
authors.forename: Mi-Yoon
authors.initials: MY
authors.lastname: Chang
authors.affiliation: Hanyang Biomedical Research Institute, Hanyang University, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Oh
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Republic of Korea; Hanyang Biomedical Research Institute, Hanyang University, Republic of Korea.
authors.forename: Yong-Hee
authors.initials: YH
authors.lastname: Rhee
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Republic of Korea; Hanyang Biomedical Research Institute, Hanyang University, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Republic of Korea. Electronic address: leesh@hanyang.ac.kr.
authors.forename: Sang-Hun
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002478:Cells, Cultured; D004318:Doxycycline / Q000008:administration & dosage / Q000627:therapeutic use*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004318:Doxycycline
keywords: Doxycycline; Human embryonic stem cell; Maintenance; Survival