title: Preparation and evaluation of amoxicillin loaded dual molecularly imprinted nanoparticles for anti-Helicobacter pylori therapy.
abstract: This paper reports studies on preparation and evaluation of amoxicillin loaded dual molecularly imprinted nanoparticles (Amo/Dual-MIPs) designed for anti-H. pylori therapy. Both MNQA and AmoNa were chosen as templates to prepare Dual-MIPs using inverse microemulsion polymerization method. NQA was modified with myristic acid (MNQA) to become amphiphilic and assist in leaving NQA cavities on the surface of Dual-MIPs for H. pylori adhesion. AmoNa was applied to produce imprinting sites in Dual-MIPs for rebinding AmoNa to exert its anti-H. pylori effect. Batch rebinding test demonstrated a preferential rebinding effect of NQA toward the Dual-MIPs. In vivofluorescence imaging showed the prolonged residence time of Dual-MIPs in H. pylori infected mice stomachs after intragastric administration of nanoparticles.In vivo H. pylori clearance tests indicated Amo/Dual-MIPs had a better aniti-H. pylori effect than amoxicillin powder did. In conclusion, Amo/Dual-MIPs may provide an alternative drug delivery strategy for anti-H. pylori therapy.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China.
authors.forename: Jiapeng
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Microbiology, Shanghai Medical College, Fudan University, Shanghai, PR China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China.
authors.forename: Miaolin
authors.initials: M
authors.lastname: Chai
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China.
authors.forename: Weiyue
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, PR China; Key Laboratory of Smart Drug Delivery (Fudan University), Ministry of Education, Shanghai, PR China. Electronic address: panjun@fudan.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Pan
mesh_terms: D000658:Amoxicillin / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D016480:Helicobacter pylori / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000008:administration & dosage*; D010316:Particle Size; D012995:Solubility
chemical_list: D000900:Anti-Bacterial Agents; D000658:Amoxicillin
keywords: Amoxicillin sodium; Dual molecularly imprinted nanoparticles; Lpp20; Template rebinding; anti-H. pylori therapy