title: A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells.
abstract: We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Yang
authors.forename: Ke
authors.initials: K
authors.lastname: Quan
authors.forename: Le
authors.initials: L
authors.lastname: Ying
authors.forename: Nuli
authors.initials: N
authors.lastname: Xie
authors.forename: Min
authors.initials: M
authors.lastname: Ou
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics; D055029:Inverted Repeat Sequences; D061986:MCF-7 Cells; D057054:Molecular Imaging; D009245:NADH Dehydrogenase / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D061848:Optical Imaging; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D000077022:Survivin; D013937:Thymidine Kinase / Q000235:genetics
chemical_list: C107489:BIRC5 protein, human; D005456:Fluorescent Dyes; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; C507225:MT-ND6 protein, human; D009245:NADH Dehydrogenase; D013937:Thymidine Kinase; C092717:thymidine kinase 1