title: Single-cell transcriptomics reveals receptor transformations during olfactory neurogenesis.
abstract: The sense of smell allows chemicals to be perceived as diverse scents. We used single-neuron RNA sequencing to explore the developmental mechanisms that shape this ability as nasal olfactory neurons mature in mice. Most mature neurons expressed only one of the ~1000 odorant receptor genes (Olfrs) available, and at a high level. However, many immature neurons expressed low levels of multiple Olfrs. Coexpressed Olfrs localized to overlapping zones of the nasal epithelium, suggesting regional biases, but not to single genomic loci. A single immature neuron could express Olfrs from up to seven different chromosomes. The mature state in which expression of Olfr genes is restricted to one per neuron emerges over a developmental progression that appears to be independent of neuronal activity involving sensory transduction molecules.
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
authors.forename: Naresh K
authors.initials: NK
authors.lastname: Hanchate
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
authors.forename: Kunio
authors.initials: K
authors.lastname: Kondoh
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
authors.forename: Donghui
authors.initials: D
authors.lastname: Kuang
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98115, USA. Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98115, USA.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Departments of Mathematics, Molecular and Cell Biology, and Electrical Engineering and Computer Sciences, University of California-Berkeley, Berkeley, CA 94720, USA.
authors.forename: Lior
authors.initials: L
authors.lastname: Pachter
authors.affiliation: Department of Genome Sciences, University of Washington, Seattle, WA 98115, USA. coletrap@uw.edu lbuck@fhcrc.org.
authors.forename: Cole
authors.initials: C
authors.lastname: Trapnell
authors.affiliation: Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA. coletrap@uw.edu lbuck@fhcrc.org.
authors.forename: Linda B
authors.initials: LB
authors.lastname: Buck
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D056426:Genetic Loci; D005819:Genetic Markers; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000378:metabolism*; D055495:Neurogenesis / Q000235:genetics*; D009831:Olfactory Mucosa / Q000294:innervation; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D018035:Receptors, Odorant / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059010:Single-Cell Analysis; D012903:Smell / Q000235:genetics*; D059467:Transcriptome
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D054815:Cyclic Nucleotide-Gated Cation Channels; D005819:Genetic Markers; D018035:Receptors, Odorant; C499317:Ascl1 protein, mouse; C518240:Cnga2 protein, mouse; C501008:Neurod1 protein, mouse