title: Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2.
abstract: We report the development of photo-crosslinked siRNA strands modified at each terminus with p-cyanostilbene. The siRNA was nuclease resistant and retained RNAi activity. We further studied the activation mechanism of the covalently-crosslinked siRNA. Interestingly Dicer, which is known to generate siRNA with overhanging 3' ends from the precursor siRNA, did not cleave the crosslinked siRNA at all. Our results suggest that the activation of the crosslinked siRNAs required cleavage by Argonaute2.
authors.affiliation: Department of Molecular Design and Engineering, Graduate school of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. asanuma@nubio.nagoya-u.ac.jp.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Kazuki
authors.initials: K
authors.lastname: Iishiba
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Doi
authors.forename: Kouki
authors.initials: K
authors.lastname: Tsuda
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kashida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Asanuma
mesh_terms: D060565:Argonaute Proteins / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D055668:Photochemical Processes; D034622:RNA Interference / Q000502:physiology / Q000528:radiation effects*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D034743:RNA-Induced Silencing Complex / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D013267:Stilbenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; D009841:Oligonucleotides; D034741:RNA, Small Interfering; D034743:RNA-Induced Silencing Complex; D013267:Stilbenes; C000613650:p-cyanostilbene