title: Pollen-mediated gene flow from transgenic cotton under greenhouse conditions is dependent on different pollinators.
abstract: With the large-scale release of genetically modified (GM) crops, there are ecological concerns on transgene movement from GM crops to non-GM counterparts and wild relatives. In this research, we conducted greenhouse experiments to measure pollen-mediated gene flow (PGF) in the absence and presence of pollinators (Bombus ignitus, Apis mellifera and Pieris rapae) in one GM cotton (resistant to the insect Helicoverpa armigera and the herbicide glyphosate) and two non-GM lines (Shiyuan321 and Hai7124) during 2012 and 2013. Our results revealed that: (1) PGF varied depending on the pollinator species, and was highest with B. ignitus (10.83%) and lowest with P. rapae (2.71%); (2) PGF with B. ignitus depended on the distance between GM and non-GM cottons; (3) total PGF to Shiyuan321 (8.61%) was higher than to Hai7124 (4.10%). To confirm gene flow, we tested hybrids carrying transgenes for their resistance to glyphosate and H. armigera, and most hybrids showed strong resistance to the herbicide and insect. Our research confirmed that PGF depended on pollinator species, distance between plants and the receptor plant.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.|National Agricultural Technology Extension and Service Center, Beijing, 100125, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.|Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, P.R. China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.
authors.forename: Weilong
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Entomology, Cornell University/New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
authors.forename: Anthony M
authors.initials: AM
authors.lastname: Shelton
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, P.R. China.
authors.forename: Junyu
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, P.R. China.
authors.forename: Jinjie
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing, 100193, P.R. China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics / Q000502:physiology; D002080:Butterflies / Q000235:genetics / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics; D017753:Ecosystem; D004780:Environment, Controlled; D051456:Gene Flow; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D003368:Gossypium / Q000145:classification / Q000235:genetics* / Q000469:parasitology; D053209:Herbicide Resistance / Q000235:genetics; D006540:Herbicides / Q000633:toxicity; D006790:Host-Parasite Interactions; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics*; D054817:Pollination / Q000502:physiology*; D013045:Species Specificity; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D000097797:Glyphosate