title: Indium-Labeling of siRNA for Small Animal SPECT Imaging.
abstract: Ever since the discovery of RNA interference (RNAi), therapeutic delivery of siRNA has attracted a lot of interest. However, due to the nature and structure of siRNA, a carrier is needed for any mode of systemic treatment. Furthermore, specific imaging techniques are required to trace where the deposition of the siRNA occurs throughout the body after treatment. Tracking in vivo siRNA biodistribution allows understanding and interpreting therapeutics effects and side effects. A great advantage of noninvasive imaging techniques such as SPECT imaging is that several time points can be assessed in the same subject. Thus, the time course of biodistribution or metabolic processes can be followed. Therefore, we have described an approach to modify siRNA with a DTPA (Diethylene Triamine Pentaacetic Acid) chelator in order to utilize an indium labeled siRNA for SPECT imaging. Here, we explain the details of the labeling and purification procedures.
authors.affiliation: Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Jones
authors.affiliation: Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA. Olivia.merkel@wayne.edu.|Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Room 3132, 259 Mack Avenue, Detroit, MI, 48201, USA. Olivia.merkel@wayne.edu.
authors.forename: Olivia
authors.initials: O
authors.lastname: Merkel
mesh_terms: D000818:Animals; D007204:Indium / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D004369:Pentetic Acid / Q000737:chemistry; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D013194:Staining and Labeling; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: D034741:RNA, Small Interfering; D007204:Indium; D004369:Pentetic Acid
keywords: DTPA; Indium labeling; Purification; SPECT imaging; siRNA; siRNA delivery; siRNA modification