title: Spatial frequency-domain multiplexed microscopy for simultaneous, single-camera, one-shot, fluorescent, and quantitative-phase imaging.
abstract: Multimodal imaging is a crucial tool when imaging biological phenomena that cannot be comprehensively captured by a single modality. Here, we introduce a theoretical framework for spatial-frequency-multiplexed microscopy via off-axis interference as a novel wide-field imaging technique that enables true simultaneous multimodal and multichannel wide-field imaging. We experimentally demonstrate this technique for single-camera, simultaneous two-channel fluorescence and one-channel quantitative-phase imaging for fluorescent microspheres and fixed cells stained for F-actin and nuclear fluorescence.
authors.forename: Shwetadwip
authors.initials: S
authors.lastname: Chowdhury
authors.forename: Will J
authors.initials: WJ
authors.lastname: Eldridge
authors.forename: Adam
authors.initials: A
authors.lastname: Wax
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Izatt
mesh_terms: D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure*; D002522:Chlorocebus aethiops; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods; D064847:Multimodal Imaging / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity