title: Rotavirus G and P types in children with acute diarrhea in Cairo, Egypt, 2011-2012.
abstract: BACKGROUND
  The WHO estimated the annual rotavirus-related mortality among children below 5 years old in Egypt in 2004 to be 30 deaths per 100 000, or out of an estimated 2616 deaths, 3.9% were because of rotavirus infection. The aim of this article was to study the epidemiology and circulating genotypes of rotaviruses in Cairo from 2011 to 2012.
  
  
  METHODS
  A total of 197 stool samples were collected from 130 inpatient children at the Cairo University Children Hospital and 67 outpatient children at the Al-Saff Children Clinic. The collected stool samples were then screened for rotavirus using enzyme immunoassay, followed by a screening for G-type and P-type using RT-PCR, and confirmation by sequence analysis.
  
  
  RESULTS
  Rotavirus was detected in 39.1% (77/197) of the children, with a higher rate in inpatients (43.9%, 57/130) than in outpatients (29.9%, 20/67). There was an increase of rotavirus infection in the winter season. The majority of rotavirus cases (85.7%) occurred during the first year of life. The predominant genotypes identified during this study were G3P[8] (37.7%) and G1P[8] (19.5%), but uncommon genotypes G1P[6] (3.9%), G9P[6] (1.3%), G8P[14] (1.3%), and G12P[6] (2.6%) were also detected.
  
  
  CONCLUSIONS
  The change in genotype distribution, compared with previous studies, along with the high burden of rotavirus-associated diarrhea among Egyptian children below 2 years old, emphasizes the importance of continuing strain surveillance and the need of developing and introducing rotavirus vaccine in Egypt.
authors.affiliation: aEnvironmental Virology Laboratory, Department of Water Pollution Research, National Research Centre, Giza, Egypt bGastroenteritis and Respiratory Viruses Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention 'CDC', Atlanta, Georgia, USA.
authors.forename: Ashraf R S
authors.initials: AR
authors.lastname: Shoeib
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Hull
authors.forename: Baoming
authors.initials: B
authors.lastname: Jiang
mesh_terms: D000554:Ambulatory Care Facilities; D001942:Breast Feeding; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D004534:Egypt / Q000453:epidemiology; D005243:Feces / Q000821:virology; D005260:Female; D005838:Genotype; D006776:Hospitals, Pediatric; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000235:genetics; D012621:Seasons