title: Application of response surface methodology for optimization of polygalacturonase production by Aspergillus niger.
abstract: Polygalacturonase (PG) degrades pectin into D-galacturonic acid monomers and is used widely in food industry especially for juice clarification. In the present study,. fermentation conditions for polygalacturonase production by Asgergillus niger NAIMCCF-02958, using mango peel as substrate, were optimized using the 2(3) factorial design with central composite rotatable experimental design (CCRD) of response surface methodology (RSM). The maximum PG activity 723.66 U g(-1) was achieved under pH 4.0, temperature 30 degrees C and 2% inoculum by response surface curve. The experimental value of PG activity wkas higher 607.65 U g(-1) than the predicted value 511.75 U g(-1). Under the proposed optimized conditions, the determination coefficient (R2) was equal to 0.66 indicating that the model could explain 66% of the total variation as well as establish the relationship between the variables and the responses. ANOVA analysis and the three dimensional plots also confirmed interactions among the parameters.
authors.forename: Kaushlesh K
authors.initials: KK
authors.lastname: Yadav
authors.forename: Neelima
authors.initials: N
authors.lastname: Garg
authors.forename: Devendra
authors.initials: D
authors.lastname: Kumar
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Achal
authors.initials: A
authors.lastname: Singh
authors.forename: M
authors.initials: M
authors.lastname: Muthukumar
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D011096:Polygalacturonase / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D011096:Polygalacturonase