title: Aptamer selection for fishing of palladium ion using graphene oxide-adsorbed nanoparticles.
abstract: A new aptamer selection method using graphene oxide (GO)-adsorbed nanoparticles (GO-adsorbed NPs) was employed for specific fishing of palladium ion. High affinity ssDNA aptamers were isolated through 13 rounds of selection and the capacity of the selected DNA aptamers for palladium ion uptake was measured, clarifying that DNA01 exhibits the highest affinity to palladium ion with a dissociation constant (Kd) of 4.60±1.17 μM. In addition, binding ability of DNA01 to palladium ion was verified against other metal ions, such as Li(+), Cs(+), Mg(2+), and Pt(2+). Results of the present study suggest that future modification of DNA01 may improve palladium ion-binding ability, leading to economic recovery of palladium from water solution.
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Yea Seul
authors.initials: YS
authors.lastname: Cho
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Gwan-Ho
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, 26 Kyunghee-daero, Dongdaemun-gu, Seoul 130-701, Republic of Korea. Electronic address: sshah@khu.ac.kr.
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Hah
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D007477:Ions; D053758:Nanoparticles / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D052156:SELEX Aptamer Technique
chemical_list: D007477:Ions; D010165:Palladium; D006108:Graphite
keywords: Adsorption; Aptamer; Graphene oxide; Palladium ion; SELEX