title: H2S, a novel gasotransmitter, involves in gastric accommodation.
abstract: H2S is produced mainly by two enzymes:cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), using L-cysteine (L-Cys) as the substrate. In this study, we investigated the role of H2S in gastric accommodation using CBS(+/-) mice, immunohistochemistry, immunoblot, methylene blue assay, intragastric pressure (IGP) recording and electrical field stimulation (EFS). Mouse gastric fundus expressed H2S-generating enzymes (CBS and CSE) and generated detectable amounts of H2S. The H2S donor, NaHS or L-Cys, caused a relaxation in either gastric fundus or body. The gastric compliance was significantly increased in the presence of L-Cys (1 mM). On the contrary, AOAA, an inhibitor for CBS, largely inhibited gastric compliance. Consistently, CBS(+/-) mice shows a lower gastric compliance. However, PAG, a CSE inhibitor, had no effect on gastric compliances. L-Cys enhances the non-adrenergic, non-cholinergic (NANC) relaxation of fundus strips, but AOAA reduces the magnitude of relaxations to EFS. Notably, the expression level of CBS but not CSE protein was elevated after feeding. Consistently, the production of H2S was also increased after feeding in mice gastric fundus. In addition, AOAA largely reduced food intake and body weight in mice. Furthermore, a metabolic aberration of H2S was found in patients with functional dyspepsia (FD). In conclusion, endogenous H2S, a novel gasotransmitter, involves in gastric accommodation.
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Xiao
authors.affiliation: Department of gastroenterology, Second Hospital, Shandong University, Jinan, People's Republic of China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Jianchun
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Tonghui
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of gastroenterology, Second Hospital, Shandong University, Jinan, People's Republic of China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Physiology, Shandong University School of Medicine, Jinan, People's Republic of China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D003541:Cystathionine beta-Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism / Q000494:pharmacology; D004415:Dyspepsia; D004435:Eating; D004558:Electric Stimulation; D064426:Gasotransmitters / Q000378:metabolism*; D005748:Gastric Fundus / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D005753:Gastric Mucosa / Q000378:metabolism*; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009119:Muscle Contraction / Q000187:drug effects; D011730:Pyridoxal / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013270:Stomach / Q000201:enzymology / Q000473:pathology; D013379:Substrate Specificity; D013440:Sulfides / Q000378:metabolism / Q000494:pharmacology; D016559:Tacrolimus / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: C112418:32-ascomycinyloxyacetic acid; D064426:Gasotransmitters; D006146:Guanidines; D013440:Sulfides; C549858:pyridoxylidene aminoguanidine; D011730:Pyridoxal; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; C025451:sodium bisulfide; D003545:Cysteine; D016559:Tacrolimus; D006862:Hydrogen Sulfide