title: In vivo cancer targeting and fluorescence-CT dual-mode imaging with nanoprobes based on silver sulfide quantum dots and iodinated oil.
abstract: In this article, a fluorescence-CT dual-mode nanoprobe is successfully synthesized by making use of distearoylphosphatidylethanolamine-poly(ethylene glycol)-folate (DSPE-PEG2000-FA) and other amphiphilic molecules to coat silver sulfide (Ag2S) quantum dots (QDs) and iodinated oil simultaneously. In vitro experiments show that the fluorescence wavelength of the nanoprobe is 1170 nm in the near infrared-II region. Its size is 139.6 nm, it has good dispersibility, and it has low cellular toxicity at concentrations up to 25 μg mL(-1) Ag. In vivo experiments revealed that the probe has a rather long circulation time (blood half-life of 5.7 hours), and the tissue histopathological tests show that it is not obviously harmful to major organs' normal function. Biochemical analysis (glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels) and blood analysis (white blood cell, red blood cell, hemoglobin and blood platelet counts) reveal that it has little influence on blood within 15 days of administration. When injected into HeLa xenograft nude mice by the tail vein, the probe elicited intensely enhanced fluorescence and X-ray computed tomography (CT) signals in the tumors after 24 hours, and the structure, size and position of tumor tissue were shown clearly. In a word, the probe has good tumor targeting capabilities, and it has significant value in fluorescence-CT dual-mode imaging in vivo.
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Meng-Yao
authors.initials: MY
authors.lastname: Qin
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Wang
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Xiao-Shuai
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Ji-Tao
authors.initials: JT
authors.lastname: Song
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Ming-Hao
authors.initials: MH
authors.lastname: Yao
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Yan
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China. zydi@mail.hust.edu.cn lbyang@mail.hust.edu.cn.
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao
mesh_terms: D000818:Animals; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007455:Iodine / Q000493:pharmacokinetics / Q000494:pharmacology; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000097:blood / Q000473:pathology; D009821:Oils / Q000493:pharmacokinetics / Q000494:pharmacology; D061848:Optical Imaging / Q000379:methods*; D045663:Quantum Dots; D018030:Silver Compounds / Q000493:pharmacokinetics / Q000494:pharmacology; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D009821:Oils; D018030:Silver Compounds; D007455:Iodine; C013251:silver sulfide