title: Occurrence, Persistence, and Virulence Potential of Listeria ivanovii in Foods and Food Processing Environments in the Republic of Ireland.
abstract: The aim of this study was to assess the occurrence of L. ivanovii in foods and food processing environments in Ireland, to track persistence, and to characterize the disease causing potential of the isolated strains. A total of 2,006 samples (432 food samples and 1,574 environmental swabs) were collected between March 2013 and March 2014 from 48 food business operators (FBOs) belonging to different production sectors (dairy, fish, meat, and fresh-cut vegetable). Six of the forty-eight FBOs had samples positive for L. ivanovii on at least one sampling occasion. L. ivanovii was present in fifteen samples (fourteen environmental samples and one food sample). All but one of those positive samples derived from the dairy sector, where L. ivanovii prevalence was 1.7%. Six distinguishable pulsotypes were obtained by PFGE analysis, with one pulsotype being persistent in the environment of a dairy food business. Sequence analysis of the sigB gene showed that fourteen isolates belonged to L. ivanovii subsp. londoniensis, while only one isolate was L. ivanovii subsp. ivanovii. Cell invasion assays demonstrated that the majority of L. ivanovii strains were comparable to L. monocytogenes EGDe in their ability to invade CACO-2 epithelial cells whilst four isolates had significantly higher invasion efficiencies.
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
authors.forename: Avelino
authors.initials: A
authors.lastname: Alvarez-Ordóñez
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
authors.forename: Dara
authors.initials: D
authors.lastname: Leong
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Ciara A
authors.initials: CA
authors.lastname: Morgan
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Colin
authors.initials: C
authors.lastname: Hill
authors.affiliation: School of Microbiology, University College Cork, Cork, Ireland.
authors.forename: Cormac G M
authors.initials: CG
authors.lastname: Gahan
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
authors.forename: Kieran
authors.initials: K
authors.lastname: Jordan
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D003611:Dairy Products / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004784:Environmental Monitoring / Q000379:methods; D004847:Epithelial Cells / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling; D019649:Food Industry; D005516:Food Microbiology; D006801:Humans; D007494:Ireland; D008087:Listeria / Q000302:isolation & purification* / Q000472:pathogenicity*; D008088:Listeriosis; D008460:Meat / Q000382:microbiology; D017747:Seafood / Q000382:microbiology; D014675:Vegetables / Q000382:microbiology; D014774:Virulence