title: Long-distance effects of inflammation on differentiation of adult spinal cord neural stem/progenitor cells.
abstract: Studies in multiple sclerosis have demonstrated that normal-appearing white matter can harbor pathological changes. Here we investigated the effects of neuroinflammation, modeled by experimental autoimmune encephalomyelitis (EAE) on neural stem/progenitor cells (NPCs) located distally to inflammatory foci. We observed that EAE-derived NPCs had a lower capacity to differentiate into oligodendrocytes and an increased neuronal differentiation than control NPCs. This finding was corroborated with changes in gene expression of early differentiation genes. We conclude that inflammation has a long range effect on the NPCs in the diseased central nervous system, reaching NPC populations outside the lesion sites.
authors.affiliation: Department of Clinical Neuroscience, Division of Neurosurgery, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Lisa
authors.initials: L
authors.lastname: Arvidsson
authors.affiliation: Division of Neurology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Ruxandra
authors.initials: R
authors.lastname: Covacu
authors.affiliation: Division of Neurology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Cynthia Perez
authors.initials: CP
authors.lastname: Estrada
authors.affiliation: Division of Neurology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Sreenivasa Raghavan
authors.initials: SR
authors.lastname: Sankavaram
authors.affiliation: Department of Clinical Neuroscience, Division of Neurosurgery, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Svensson
authors.affiliation: Division of Neurology, Karolinska Institutet, Stockholm, Sweden. Electronic address: lou.brundin@karolinska.se.
authors.forename: Lou
authors.initials: L
authors.lastname: Brundin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007249:Inflammation / Q000150:complications*; D018613:Microscopy, Confocal; D058953:Neural Stem Cells / Q000473:pathology*; D009474:Neurons / Q000473:pathology; D009836:Oligodendroglia / Q000473:pathology; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D013116:Spinal Cord / Q000473:pathology*
keywords: Adult neural progenitor cells; Gene expression; Gliogenesis; Neurogenesis; Neuroinflammation; Proliferation; Spinal cord