title: [Sequence and Structural Analyses of the Complete Genome of Bovine Papillomavirus 2 Genotype Aks-01 Strain from Skin Samples of Cows in Southern Xinjiang, China].
abstract: To study the complete genomic sequence, genomic characteristics, and genetic variation of the bovine papillomavirus 2 genotype (BPV-2) Aks-01 strain at the molecular level, genotyping of this strain from the skin samples of cows in southern Xinjiang (China) was first detected by the polymerase chain reaction with FAP59/FAP64 primers. Based on the complete genome of the BPV-2 reference strain, specific primers and sequencing primers were designed, and the complete genome of the Aks-01 strain amplified and sequenced. Sequence analyses showed that genotyping of the Aks-01 strain belonged to BPV-2. The Aks-01 strain had the structural characteristics of BPV-2. The 7944-bp full-length genomic sequence of the Aks-01 strain was compiled using DNAStar™. The sequence of the Aks-01 strain had 98% similarity to the reference strain from GenBank. The Aks-01 strain was most closely related to BPV-1 and BPV-13. BPV-2, BPV-1 and BPV-13 were grouped within the genus Deltapapillomavirus. The Aks-01 strain is the first BPV-2 strain reported in southern Xinjiang.
authors.forename: Wanqi
authors.initials: W
authors.lastname: Zhang
authors.forename: Jianjun
authors.initials: J
authors.lastname: Hu
authors.forename: Shilei
authors.initials: S
authors.lastname: Yan
authors.forename: Yaojie
authors.initials: Y
authors.lastname: Huang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xu
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Huang
authors.forename: Maoliang
authors.initials: M
authors.lastname: Zheng
authors.forename: Ziyan
authors.initials: Z
authors.lastname: Meng
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D002681:China; D019143:Evolution, Molecular; D005260:Female; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics; D005838:Genotype; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012867:Skin / Q000821:virology*
chemical_list: D009856:Oncogene Proteins, Viral; C075621:oncogene protein E7, Bovine papillomavirus type 1