title: Spatial control of chromosomal location in a live cell with functionalized magnetic particles.
abstract: Long-range chromosomal travel is a phenomenon unique to cell division. Methods for non-invasive, artificial manipulation of chromosomes, such as optical or magnetic tweezers, have difficulty in producing the motion of whole chromosomes in live cells. Here, we report the spatial control of chromosomes over 10 μm in a live mouse oocyte using magnetic particles driven by an external magnetic field. Selective capture of the chromosomes was achieved using antibodies specific for histone H1 in the chromosome that were conjugated to magnetic particles (H1-BMPs). When an external magnetic field was applied, the chromosomes captured by the H1-BMPs traveled through the cytosol and accumulated near the cell membrane though the movement of the chromosomes captured by H1-BMPs was strongly disturbed by the distribution of the cytoskeleton (e.g. actin filaments). Being non-invasive in nature, our approach will enable new opportunities in the remote manipulation of subcellular elements.
authors.affiliation: School of Mechanical and Aerospace Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-744, South Korea. jleenano@snu.ac.kr.
authors.forename: Juhee
authors.initials: J
authors.lastname: Hong
authors.forename: Prashant
authors.initials: P
authors.lastname: Purwar
authors.forename: Misun
authors.initials: M
authors.lastname: Cha
authors.forename: Junghoon
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D005260:Female; D006657:Histones / Q000378:metabolism; D060526:Magnetic Fields; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D006657:Histones