title: Release kinetics of circulating miRNA-208a   in the early phase of myocardial infarction.
abstract: BACKGROUND
  The biochemical confirmation of myocardial infarction is based on cardiac troponin (cTnI or cTnT) determination. Recent scientific results suggested that microRNAs (miRNAs) might become a new biomarker of tissue injury.
  
  
  OBJECTIVE
  To evaluate the release kinetics of circulating heart-specific miRNA-208a and also to test the hypothesis that miRNA-208a can serve as an accessible, diagnostically sensitive plasma biomarker of ST-elevation acute myocardial infarction (STEMI).
  
  
  METHODS
  Nineteen STEMI patients (four women and 15 men, aged 44-85 years), 12 patients with stable coronary artery disease (CAD), and eight patients with a negative observation of CAD as a control group were studied. Blood samples were collected on admission and at three, six, 12, 24, and 48 h afterwards; in the CAD and control group blood samples were taken only once. Plasma levels of miRNA-208a determined by real-time polymerase chain reaction and their relative fold changes were calculated. cTnI and creatinine kinase (CK)-MB mass were also measured in the patients’ serum samples.
  
  
  RESULTS
  miRNA-208a was increased in STEMI patients at the time of admission and nearly undetectable in CAD patients and controls. The peak of miRNA-208a was observed at 3 h after reperfusion (p < 0.001). The traditional biomarkers (cTnI and CK-MBmass), which increase later in comparison to miRNA-208a reaching the maximum concentrations 6 h after reperfusion, were observed. Circulating miRNA-208a levels strongly correlated with cTnI and CK-MBmass released from the infarcted area.
  
  
  CONCLUSIONS
  These results demonstrate that plasma miRNA-208a is an interesting and promising candidate for a new biomarker released early after onset of myocardial infarction.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Białek
authors.forename: Dariusz
authors.initials: D
authors.lastname: Górko
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zajkowska
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Kołtowski
authors.forename: Marcin
authors.initials: M
authors.lastname: Grabowski
authors.forename: Anna
authors.initials: A
authors.lastname: Stachurska
authors.forename: Janusz
authors.initials: J
authors.lastname: Kochman
authors.forename: Grażyna
authors.initials: G
authors.lastname: Sygitowicz
authors.forename: Maciej
authors.initials: M
authors.lastname: Małecki
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Opolski
authors.forename: Dariusz
authors.initials: D
authors.lastname: Sitkiewicz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000378:metabolism; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; C550906:MIRN208 microRNA, human; D035683:MicroRNAs