title: Metallothionein gene expression in embryos of the terrestrial snail (Cantareus aspersus) exposed to cadmium and copper in the Bordeaux mixture.
abstract: The response specificity of three metallothionein (MT) genes (CdMT, CuMT and Cd/CuMT) was assessed after long-term exposure (20 days) of Cantareus aspersus eggs to cadmium (Cd) (2 to 6 mg/L) or to the fungicide Bordeaux mixture (BM) (2.5 and 7.5 g/L). MT gene expression measured by quantitative real-time PCR (qRT-PCR) revealed that in the unexposed embryos, the transcript levels of the three MT genes decreased significantly through embryonic development. However, the CdMT gene was strongly upregulated with increasing Cd exposure concentration, whereas the transcript levels of the other two genes increased less pronouncedly, but significantly above an exposure concentration of 4 mg Cd/L. Upon exposure to BM, all three MT genes were significantly upregulated above a BM concentration of 2.5 g/L. It is concluded that long-term Cd exposure in hatched snails induced patterns of MT gene expression that differed from those obtained after short-term exposure (24 h).
authors.affiliation: Chrono-Environnement, UMR 6249 University of Bourgogne Franche-Comté, 16 route de Gray, 25000, Besançon, France.
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Baurand
authors.affiliation: Institute of Zoology, University of Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Dallinger
authors.affiliation: Chrono-Environnement, UMR 6249 University of Bourgogne Franche-Comté, 16 route de Gray, 25000, Besançon, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Capelli
authors.affiliation: Chrono-Environnement, UMR 6249 University of Bourgogne Franche-Comté, 16 route de Gray, 25000, Besançon, France. annette.devaufleury@univ-fcomte.fr.|Department of Health Safety Environment, avenue des Rives du Lac, BP179, 70003, Vesoul cedex, France. annette.devaufleury@univ-fcomte.fr.
authors.forename: Annette
authors.initials: A
authors.lastname: de Vaufleury
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D008668:Metallothionein / Q000235:genetics*; D012908:Snails / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D002104:Cadmium; D003300:Copper; C006788:Bordeaux mixture; D008668:Metallothionein
keywords: Bordeaux mixture; Detoxification; Embryotoxicity