title: Control of enzymatic degradation of biodegradable polymers by treatment with biosurfactants, mannosylerythritol lipids, derived from Pseudozyma spp. yeast strains.
abstract: Cutinase-like esterase from the yeasts Pseudozyma antarctica (PaE) shows strong degradation activity in an agricultural biodegradable plastic (BP) model of mulch films composed of poly(butylene succinate-co-adipate) (PBSA). P. antarctica is known to abundantly produce a glycolipid biosurfactant, mannosylerythritol lipid (MEL). Here, the effects of MEL on PaE-catalyzed degradation of BPs were investigated. Based on PBSA dispersion solution, the degradation of PBSA particles by PaE was inhibited in the presence of MEL. MEL behavior on BP substrates was monitored by surface plasmon resonance (SPR) using a sensor chip coated with polymer films. The positive SPR signal shift indicated that MEL readily adsorbed and spread onto the surface of a BP film. The amount of BP degradation by PaE was monitored based on the negative SPR signal shift and was decreased 1.7-fold by MEL pretreatment. Furthermore, the shape of PBSA mulch films in PaE-containing solution was maintained with MEL pretreatment, whereas untreated films were almost completely degraded and dissolved. These results suggest that MEL covering the surface of BP film inhibits adsorption of PaE and PaE-catalyzed degradation of BPs. We applied the above results to control the microbial degradation of BP mulch films. MEL pretreatment significantly inhibited BP mulch film degradation by both PaE solution and BP-degradable microorganism. Moreover, the degradation of these films was recovered after removal of the coated MEL by ethanol treatment. These results demonstrate that the biodegradation of BP films can be readily and reversibly controlled by a physical approach using MEL.
authors.affiliation: Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kan-nondai, Tsukuba, Ibaraki, 305-8604, JAPAN.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Shinozaki
authors.affiliation: Biomolecular Research Unit, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kan-nondai, Tsukuba, Ibaraki, 305-8602, JAPAN.
authors.forename: Wataru
authors.initials: W
authors.lastname: Tsuchiya
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kan-nondai, Tsukuba, Ibaraki, 305-8604, JAPAN.
authors.forename: Ken
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kan-nondai, Tsukuba, Ibaraki, 305-8604, JAPAN.
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Biomolecular Research Unit, National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kan-nondai, Tsukuba, Ibaraki, 305-8602, JAPAN.
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
authors.affiliation: National Institute for Agro-Environmental Sciences (NIAES), 3-1-3 Kan-nondai, Tsukuba, Ibaraki, 305-8604, JAPAN. kitamoto@affrc.go.jp.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kitamoto
mesh_terms: D000272:Adipates / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects; D006017:Glycolipids / Q000378:metabolism*; D006868:Hydrolysis; D013386:Succinates / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013501:Surface-Active Agents / Q000378:metabolism*; D014587:Ustilaginales / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D000272:Adipates; D006017:Glycolipids; D013386:Succinates; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; C437084:poly(tetramethylene succinate-co-tetramethylene adipate)
keywords: Biodegradable plastics; Biosurfactants; Mannosylerythritol lipid; PaE; Pseudozyma yeast