title: Immune parameters differentiating active from latent tuberculosis infection in humans.
abstract: Tuberculosis remains a highly prevalent infectious disease worldwide. Identification of the immune parameters that differentiate active disease from latent infection will facilitate the development of efficient control measures as well as new diagnostic modalities for tuberculosis. Here, we investigated the cytokine production profiles of monocytes and CD4(+) T lymphocytes upon encountering mycobacterial antigens. In addition, cytokines and lipid mediators with immune-modulating activities were examined in plasma samples ex vivo. Comparison of these parameters in active tuberculosis patients and healthy subjects with latent infection revealed that, active tuberculosis was associated with diminished Th1-type cytokine secretion from CD4(+) T cells and less augmented inflammatory cytokine secretion from monocytes induced by IFN-γ than that in latent tuberculosis infection. In addition, a higher plasma concentration of lipoxin A4 and lower ratio of prostaglandin E2 to lipoxin A4 were observed in active cases than in latent infections. These findings have implications for preparing new therapeutic strategies and for differential diagnosis of the two types of tuberculosis infection.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Jung-gu Community Health Center, Seoul 132-713, Republic of Korea.
authors.forename: Young Won
authors.initials: YW
authors.lastname: Jung
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Ina
authors.initials: I
authors.lastname: Jeong
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Joon-Sung
authors.initials: JS
authors.lastname: Joh
authors.affiliation: Center for Chronic Diseases, Research Institute, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Soo Yeon
authors.initials: SY
authors.lastname: Sim
authors.affiliation: Center for Chronic Diseases, Research Institute, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Choi
authors.affiliation: Center for Chronic Diseases, Research Institute, National Medical Center, Seoul 100-799, Republic of Korea.
authors.forename: Hyeon-Gun
authors.initials: HG
authors.lastname: Jee
authors.affiliation: Center for Chronic Diseases, Research Institute, National Medical Center, Seoul 100-799, Republic of Korea. Electronic address: dglim@nmc.or.kr.
authors.forename: Dong-Gyun
authors.initials: DG
authors.lastname: Lim
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000097:blood / Q000276:immunology*; D003937:Diagnosis, Differential; D015232:Dinoprostone / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D055985:Latent Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D044045:Lipoxins / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011237:Predictive Value of Tests; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D016207:Cytokines; D044045:Lipoxins; C040527:lipoxin A4; D015232:Dinoprostone
keywords: CD4(+) T cells; Cytokine; Eicosanoids; Monocytes; Tuberculosis