title: Structural advances on titin: towards an atomic understanding of multi-domain functions in myofilament mechanics and scaffolding.
abstract: Titin is a gigantic filamentous protein of the muscle sarcomere that plays roles in myofibril mechanics and homoeostasis. 3D-structures of multi-domain fragments of titin are now available that start revealing the molecular mechanisms governing its mechanical and scaffolding functions. This knowledge is now being translated into the fabrication of self-assembling biopolymers. Here we review the structural advances on titin, the novel concepts derived from these and the emerging translational avenues.
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zacharchenko
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
authors.forename: Eleonore
authors.initials: E
authors.lastname: von Castelmur
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Rigden
authors.affiliation: Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, U.K. Olga.Mayans@liv.ac.uk.
authors.forename: Olga
authors.initials: O
authors.lastname: Mayans
mesh_terms: D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry; D064211:Connectin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D009210:Myofibrils / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012518:Sarcomeres / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D064211:Connectin; D011993:Recombinant Fusion Proteins; C577292:TCAP protein, human; C577285:TTN protein, human
keywords: crystal structure; multi-Ig tandem; protein nanofibre; protein scaffold; titin