title: Effects of μ-Opioid Receptor Agonist DAMGO on Heart Contractility and Necrotic Injury to Cardiomyocytes during Ischemia and Reperfusion of Isolated Rat Heart.
abstract: We studied the effects of μ-opioid receptor activation in vivo and in vitro on the tolerance of isolated perfused rat heart to global ischemia (45 min) and reperfusion (30 min). Stimulation of μ-receptors in vivo by intraperitoneal administration of μ-opioid receptor agonist DAMGO (0.1 mg/kg) reduced reperfusion release of creatinine phosphokinase and promoted aggravation of postischemic systolic and diastolic dysfunction of the isolated heart. Activation of μ-opioid receptors in vitro by addition of selective agonist DAMGO in a concentration of 170 nM to perfusion solution had no effect on necrotic death of cardiomyocytes and aggravated reperfusion stunning of the heart.
authors.affiliation: Department of Medical and Biological Disciplines, Tomsk State Pedagogical University, Tomsk, Russia. tlasukova@mail.ru.|Laboratory of Experimental Cardiology, Research Institute of Cardiology, Tomsk, Russia. tlasukova@mail.ru.
authors.forename: T V
authors.initials: TV
authors.lastname: Lasukova
authors.affiliation: Laboratory of Experimental Cardiology, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: L N
authors.initials: LN
authors.lastname: Maslov
authors.affiliation: Laboratory of Experimental Cardiology, Research Institute of Cardiology, Tomsk, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Gorbunov
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000494:pharmacology* / Q000627:therapeutic use; D006321:Heart / Q000187:drug effects / Q000502:physiology; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000473:pathology*; D009336:Necrosis; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000819:agonists; D015424:Reperfusion
chemical_list: D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
keywords: heart; ischemia; opioid receptors; reperfusion