title: GENETIC SUBTYPES OF BLASTOCYSTIS ISOLATED FROM THAI HOSPITALIZED PATIENTS IN NORTHEASTERN THAILAND.
abstract: Blastocystis sp is probably the most common intestinal protozoan of humans. This taxon is known to include more than 17 subtypes, some of which likely cause human disease. We investigated the distribution of Blastocystis subtypes in Thai patients admitted for a variety of conditions at a hospital in northeastern Thailand. Fresh fecal samples, positive for Blastocystis by microscopy, were individually cultured in Jones' medium (n = 20) and each sample was used for amplification and sequencing a fragment of 18S rDNA. BLAST search and phylogenetic analysis demonstrated that Blastocystis subtypes ST1 (20%), ST3 (60%), ST6 (10%) and ST7 (10%) were present. No clear link between gastro-intestinal symptoms and any particular subtype of Blastocystis was apparent. Thus, there is a need to extend the work to evaluate clinical signs and subtypes in a larger cohort of patients.
authors.forename: Oranuch
authors.initials: O
authors.lastname: Sanpool
authors.forename: Pokkamol
authors.initials: P
authors.lastname: Laoraksawong
authors.forename: Penchom
authors.initials: P
authors.lastname: Janwan
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.forename: Kittisak
authors.initials: K
authors.lastname: Sawanyawisuth
authors.forename: Tongjit
authors.initials: T
authors.lastname: Thanchomnang
authors.forename: Yossombat
authors.initials: Y
authors.lastname: Changtrakul
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016844:Blastocystis / Q000235:genetics*; D016776:Blastocystis Infections / Q000453:epidemiology / Q000469:parasitology*; D002648:Child; D015331:Cohort Studies; D004275:DNA, Ribosomal / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D005260:Female; D006761:Hospitals; D006801:Humans; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013785:Thailand / Q000453:epidemiology; D055815:Young Adult
chemical_list: D004275:DNA, Ribosomal