title: Phenolic constituents of the Bangladeshi medicinal plant Pothos scandens and their anti-estrogenic, hyaluronidase inhibition, and histamine release inhibitory activities.
abstract: Extracts from the stem and roots of the Bangladeshi medicinal plant Pothos scandens L. (Araceae) were isolated, and three hemiterpene glucoside aromatic esters, pothobanosides A (1), B (2), and C (3), and a phenylisobutanoid, pothobanol (4), along with 14 known compounds, were characterized. The isolates were tested for their estrogenic/anti-estrogenic activity using the estrogen-responsive human breast cancer cell lines MCF-7 and T47D, and syringoyl derivatives (2, 3, and canthoside B) showed strong inhibitory activity against both cell lines. Their less oxygenated analogs (1, and markhamioside F) were almost inactive. The isolates were also evaluated for hyaluronidase and histamine release inhibitory activities, and pothobanoside A (1) showed significant hyaluronidase inhibitory activity among the isolated compounds, which was similar to that of the positive control rosmarinic acid. Because hyaluronidase produces an angiogenic response that has been implicated in tumor invasiveness and metastasis, 1 could be valuable as an anti-tumor compound with a different mechanism of action from related compounds (2, 3). Pothobanoside C (3) and pothobanol (4) were also found to inhibit histamine release to a similar degree to the positive control epigallocatechin 3-O-(3"-O-methyl)-gallate. The histamine release inhibitory potency of these isolates may support the traditional uses of this plant in folk medicine.
authors.affiliation: School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan; Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
authors.forename: Md Abdul
authors.initials: MA
authors.lastname: Muhit
authors.affiliation: School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Izumikawa
authors.affiliation: School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan. Electronic address: umehara@u-shizuoka-ken.ac.jp.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Umehara
authors.affiliation: School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka 422-8526, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
mesh_terms: D029064:Araceae / Q000737:chemistry*; D001459:Bangladesh; D002073:Butanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D005960:Glucosides; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D045782:Hemiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D002073:Butanes; D004965:Estrogen Antagonists; D005960:Glucosides; D006027:Glycosides; D045782:Hemiterpenes; D010636:Phenols; C000609203:pothobanol; C000609200:pothobanoside A; C000609201:pothobanoside B; C000609202:pothobanoside C; D006821:Hyaluronoglucosaminidase
keywords: Anti-estrogenic activity; Araceae; Hemiterpene; Histamine release inhibitory activity; Hyaluronidase inhibitory activity; Pothobanol; Pothobanoside; Pothos scandens; Syringoyl