title: In vivo evidence for chylomicrons as mediators of postprandial inflammation.
abstract: OBJECTIVE
  The postprandial situation is a pro-inflammatory condition most likely linked to the development of atherosclerosis. We evaluated the relationship between apolipoprotein (apo) B48 and fasting and postprandial leukocyte activation markers.
  
  
  METHODS
  Leukocyte activation markers and apo B48 were determined in 80 subjects with and without coronary artery disease (CAD). Twelve healthy subjects underwent an oral fat loading test (up to 8 h).
  
  
  RESULTS
  Fasting apo B48 was significantly higher in patients with CAD (n = 47, 8.1 ± 5.2 mg/L) than in subjects without CAD (n = 33, 5.9 ± 3.9 mg/L, p = 0.022). Fasting apo B48 and triglycerides correlated positively with fasting monocyte CD11b and neutrophil CD66b expression. Plasma apo B48 and leukocyte activation markers increased after an oral fat load. No correlations were found between fasting or postprandial triglycerides and postprandial leukocyte activation markers. We observed no correlations between postprandial apo B48 and postprandial neutrophil CD11b or CD66b expression.
  
  
  CONCLUSIONS
  This study suggests that chylomicron remnants may be responsible for postprandial leukocyte activation in the circulation. The postprandial chylomicron response may be a stronger mediator of postprandial inflammation than postprandial triglyceridemia.
authors.affiliation: Department of Internal Medicine, Center for Diabetes and Vascular Medicine, Rotterdam, The Netherlands.
authors.forename: Marijke A
authors.initials: MA
authors.lastname: de Vries
authors.affiliation: Department of Internal Medicine, Center for Diabetes and Vascular Medicine, Rotterdam, The Netherlands.
authors.forename: Boudewijn
authors.initials: B
authors.lastname: Klop
authors.affiliation: Department of Internal Medicine, Center for Diabetes and Vascular Medicine, Rotterdam, The Netherlands.
authors.forename: Arash
authors.initials: A
authors.lastname: Alipour
authors.affiliation: Department of Clinical Chemistry, Rotterdam, The Netherlands.
authors.forename: Gert-Jan M
authors.initials: GJ
authors.lastname: van de Geijn
authors.affiliation: Department of Clinical Chemistry, Rotterdam, The Netherlands.
authors.forename: Lenneke
authors.initials: L
authors.lastname: Prinzen
authors.affiliation: Department of Cardiology, Sint Franciscus Gasthuis, Rotterdam, The Netherlands.
authors.forename: Anho H
authors.initials: AH
authors.lastname: Liem
authors.affiliation: Internal Medicine Unit, Hospital Virgen de la Victoria and Department of Medicine and Dermatology, IBIMA, University Málaga, Campus de Teatinos s/n, 29010 Málaga, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Valdivielso
authors.affiliation: Internal Medicine Unit, Hospital Virgen de la Victoria and Department of Medicine and Dermatology, IBIMA, University Málaga, Campus de Teatinos s/n, 29010 Málaga, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Rioja Villodres
authors.affiliation: Internal Medicine Unit, Hospital Virgen de la Victoria and Department of Medicine and Dermatology, IBIMA, University Málaga, Campus de Teatinos s/n, 29010 Málaga, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Ramírez-Bollero
authors.affiliation: Department of Internal Medicine, Center for Diabetes and Vascular Medicine, Rotterdam, The Netherlands. Electronic address: m.castrocabezas@sfg.nl.
authors.forename: Manuel
authors.initials: M
authors.lastname: Castro Cabezas
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood; D053283:Apolipoprotein B-48 / Q000097:blood; D039481:CD11b Antigen / Q000097:blood; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000097:blood; D053298:Chylomicron Remnants / Q000097:blood*; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D003430:Cross-Sectional Studies; D004041:Dietary Fats / Q000008:administration & dosage / Q000097:blood*; D005260:Female; D058851:GPI-Linked Proteins / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood* / Q000175:diagnosis / Q000209:etiology; D018836:Inflammation Mediators / Q000097:blood*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D012307:Risk Factors; D015398:Signal Transduction; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D015703:Antigens, CD; D053283:Apolipoprotein B-48; D039481:CD11b Antigen; C504120:CEACAM8 protein, human; D015815:Cell Adhesion Molecules; D053298:Chylomicron Remnants; D004041:Dietary Fats; D058851:GPI-Linked Proteins; C509295:ITGAM protein, human; D018836:Inflammation Mediators; D014280:Triglycerides
keywords: Apolipoprotein B48; Apolipoproteins; Atherosclerosis; Leukocyte activation; Monocytes; Neutrophils; Postprandial lipemia; Triglycerides