title: Ethyl acetate Salix alba leaves extract-loaded chitosan-based hydrogel film for wound dressing applications.
abstract: High toxicity and multidrug resistance associated with various standard antimicrobial drugs have necessitated search for safer alternatives in plant-derived materials. In this study, we performed biological examination of chitosan-based hydrogel film loaded with ethyl acetate Salix alba leaves extract against 11 standard laboratory strains. FTIR showed regeneration of saccharide peak in CP1A at 1047 cm(-1) and increased in height of other peaks. DSC exothermic decomposition peaks at 112 °C, 175 °C and 251 °C reveal the effect of extract on hydrogel film. From FESEM images, three-dimensional cross-linking and extract easily seen in the globular form from the surface. MTT assay on HEK 293 cells showed that CP1A was non-toxic. Minimum inhibitory concentration ranges from 4000 μg/ml to 125 μg/ml. Enterococcus faecium, Candida glabrata and Candida tropicalis were the most resistant, while Salmonella typhi and Candida guilliermondii were the most susceptible micro-organisms.
authors.affiliation: a Faculty of Engineering & Technology, Department of Applied Sciences & Humanities , Jamia Millia Islamia , New Delhi 110025 , India.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Qureshi
authors.affiliation: a Faculty of Engineering & Technology, Department of Applied Sciences & Humanities , Jamia Millia Islamia , New Delhi 110025 , India.
authors.forename: Fehmeeda
authors.initials: F
authors.lastname: Khatoon
authors.affiliation: b Faculty of Natural Science, Department of Bioscience , Jamia Millia Islamia , New Delhi 110025 , India.
authors.forename: Moshahid A
authors.initials: MA
authors.lastname: Rizvi
authors.affiliation: b Faculty of Natural Science, Department of Bioscience , Jamia Millia Islamia , New Delhi 110025 , India.
authors.forename: Md
authors.initials: M
authors.lastname: Zafaryab
mesh_terms: D000085:Acetates / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D047070:Bandages, Hydrocolloid; D002175:Candida / Q000187:drug effects; D041221:Candida glabrata / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D016984:Enterococcus faecium / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D008422:Materials Testing; D008756:Methylgalactosides / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D032108:Salix; D012485:Salmonella typhi / Q000187:drug effects; D013499:Surface Properties
chemical_list: D000085:Acetates; D000900:Anti-Bacterial Agents; D008756:Methylgalactosides; D010936:Plant Extracts; C109801:hydrogel film; C007650:ethyl acetate; D048271:Chitosan
keywords: Salix alba; antimicrobial; chitosan; poly vinyl pyrolidone