title: Screening bactericidal effect of Pectobacterium carotovorum subsp. carotovorum strains against causal agent of potato soft rot.
abstract: This study focuses on the potential of Pectobacterium carotovorum subsp. carotovorum (Pcc) strains producing bacteriocin as a tool to control potato soft rot disease. Thirty out of 48 purified bacterial strains were characterized as Pcc using specific PCR and phenotypic tests. The pathogenicity and pectate degrading assays were recorded positive for 13 strains. Bacteriocin typing clustered producers into three groups according to their antimicrobial spectra. Majority of the producers except strains of group II showed antibacterial activity toward relative genus and the role of UV or mitomycin C was inductive. In addition, none of the distant genus was sensitive to Pcc bacteriocins except Rhizobium vitis. Molecular detection of four bacteriocins including carotovoricin, carosin S1, S2 and carosin D was performed. Overall, 54.5% of group I, 47.3 and 70% of groups II and III strains carried carotovoricin and four strains harbored gene corresponding to carosin S1. According to our data divers antimicrobial patterns obtained by Pcc strains and existence of new bateriocines could be possible. Moreover, our findings recommended that direct application of P29 or expression of corresponding genes of Pog22 or P21 in a nonpathogenic strain as a biocontrol agent may improve soft rot disease control.
authors.affiliation: Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Kazemi-Zaromi
authors.affiliation: Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Sareh
authors.initials: S
authors.lastname: Baghaee-Ravari
authors.affiliation: Department of Plant Protection, Faculty of Agriculture, Vali-E-Asr University, Rafsanjan, Iran.
authors.forename: Pejman
authors.initials: P
authors.lastname: Khodaygan
authors.affiliation: Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Mahrokh
authors.initials: M
authors.lastname: Falahati-Rastegar
mesh_terms: D000898:Antibiosis / Q000187:drug effects* / Q000528:radiation effects*; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism*; D008403:Mass Screening; D016685:Mitomycin / Q000378:metabolism; D016973:Pectobacterium carotovorum / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control*; D011198:Solanum tuberosum / Q000382:microbiology*; D014466:Ultraviolet Rays
chemical_list: D001430:Bacteriocins; D016685:Mitomycin
keywords: Control; Inducer; Inhibitory effect; Tuber decay