title: Abnormal Expression of Sg I is Closely Related to Seminal Vesiculitis.
abstract: OBJECTIVE
  To investigate firstly the relationship between semenogelin I (Sg I) expression and seminal vesiculitis. Seminal vesiculitis is one of the most common diseases in male urogenital system. However, the cause and the pathogenesis of seminal vesiculitis remain unknown. Sg I, mainly synthesized and secreted by seminal vesicle, is abundant in human seminal plasma and has antibacterial activity.
  
  
  METHODS
  Tissue samples were collected from 15 normal cases and 28 patients with seminal vesiculitis. Reverse transcription-polymerase chain reaction was performed to detect the expression difference of Sg I messenger ribonucleic acid (RNA) between normal seminal vesicle tissues and seminal vesiculitis tissues. Immunohistochemistry was applied to detect the expression difference of Sg I protein between the 2 groups.
  
  
  RESULTS
  Reverse transcription-polymerase chain reaction showed the expression of Sg I messenger RNA in seminal vesiculitis tissues to be significantly lower than in normal seminal vesicle tissues. In most cases with seminal vesiculitis (78.6%), the same result was observed upon immunohistochemical analysis at the protein level.
  
  
  CONCLUSIONS
  Abnormal expression of Sg I is closely related to seminal vesiculitis. Low expression of Sg I may play an important role in the occurrence and the development of seminal vesiculitis through weakening the antibacterial activity of seminal vesicle.
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Bianjiang
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Urology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Su
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Aiming
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Ninghong
authors.initials: N
authors.lastname: Song
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Gu
authors.affiliation: Department of Urology and State Key Laboratory of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address: zengjunwang@njmu.edu.cn.
authors.forename: Zengjun
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D029607:Seminal Vesicle Secretory Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D012669:Seminal Vesicles; D055815:Young Adult
chemical_list: D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen