title: Over-expressed RPL34 promotes malignant proliferation of non-small cell lung cancer cells.
abstract: Ribosomal protein L34 (RPL34) was reported to be involved in the regulation of cell proliferation of prokaryotes, plant and animal cells. In the present study, we analyze the expression and function of RPL34 in NSCLC. Immunohistochemical analysis, qPCR and Western blot were used to detect the expression of RPL34 in NSCLC tissues and cells lines. Flow cytometry was used to detect cell activity of NSCLC cell line H1299 under lentivirus-mediated RNAi on RPL34. Cell proliferation and colony formation assays were used to analyze the role of RPL34 in NSCLC cell proliferation. We found that expression of ribosomal protein RPL34 was significantly up-regulated in NSCLC tissues compared to adjacent normal tissues. Lentivirus-mediated shRNA knockdown of RPL34 in NSCLC cell line H1299 resulted in a strong decrease of proliferation, and a moderate but significant increase of apoptosis and S-phase arrest. These data indicate that over-expressed RPL34 may promote malignant proliferation of NSCLC cells, thus playing an important role in development and progress of NSCLC.
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Shaoxing
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery, Haidian Hospital, No. 29 Zhongguancun Ave., Haidian District, Beijing 100080, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Thoracic Surgery, Haidian Hospital, No. 29 Zhongguancun Ave., Haidian District, Beijing 100080, China.
authors.forename: Yingshun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemotherapy, Cixian Tumor Hospital, No. 15 Cizhou Road, Cixian, Hebei 056500, China.
authors.forename: Zhaoping
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Chemotherapy, Cixian Tumor Hospital, No. 15 Cizhou Road, Cixian, Hebei 056500, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Yan
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Chuanhao
authors.initials: C
authors.lastname: Tang
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China.
authors.forename: Weixia
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Haidian Hospital, No. 29 Zhongguancun Ave., Haidian District, Beijing 100080, China. Electronic address: huangyq77@163.com.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Pulmonary Oncology, Affiliated Hospital, Academy of Military Medical Science, No. 8 East Avenue, Fengtai District, Beijing 100071, China. Electronic address: gaohj001@163.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D012269:Ribosomal Proteins / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D012269:Ribosomal Proteins; C041672:ribosomal protein L34
keywords: Cell proliferation; NSCLC; RPL34; Ribosomal protein