title: Combined High-Density Lipoprotein Proteomic and Glycomic Profiles in Patients at Risk for Coronary Artery Disease.
abstract: OBJECTIVE
  To test whether recently developed methods for comprehensive profiling of the high-density lipoprotein (HDL) glycome combined with the HDL proteome can distinguish individuals with coronary artery disease (CAD) from those without.
  
  
  METHODS
  Twenty subjects at risk for CAD, who underwent diagnostic coronary arteriography, were analyzed. Ten subjects had CAD, and ten did not. HDL was extracted from fasting plasma samples by ultracentrifugation, followed by shotgun proteomic, glycomic, and ganglioside analyses using LC-MS. CAD vs non-CAD subjects' data were compared using univariate and multivariate statistics.
  
  
  RESULTS
  Principal components analysis showed a clear separation of CAD and non-CAD subjects, confirming that combined HDL proteomic and glycomic profiles distinguished at-risk subjects with atherosclerosis from those without. CAD patients had lower HDL apolipoprotein content (specifically ApoA-I, A-II, and E, p < 0.05), and lower serum amyloid A2 (SAA2, p = 0.020) and SAA4 (p = 0.007) but higher sialylated glycans (p < 0.05).
  
  
  CONCLUSIONS
  Combined proteomic and glycomic profiling of isolated HDL was tested as a novel analytical approach for developing biomarkers of disease. In this pilot study we found that HDL proteome and glycome distinguished between individuals who had CAD from those who did not within a group of individuals equally at risk for heart disease.
authors.forename: Sridevi
authors.initials: S
authors.lastname: Krishnan
authors.forename: Jincui
authors.initials: J
authors.lastname: Huang
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Andres
authors.initials: A
authors.lastname: Guerrero
authors.affiliation: Department of Veterans Affairs, Northern California Health Care System , Sacramento, California 95655, United States.
authors.forename: Lars
authors.initials: L
authors.lastname: Berglund
authors.forename: Erdembileg
authors.initials: E
authors.lastname: Anuurad
authors.forename: Carlito B
authors.initials: CB
authors.lastname: Lebrilla
authors.forename: Angela M
authors.initials: AM
authors.lastname: Zivkovic
mesh_terms: D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000097:blood; D016022:Case-Control Studies; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis; D005260:Female; D005732:Gangliosides / Q000032:analysis; D054794:Glycomics / Q000379:methods; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D012307:Risk Factors
chemical_list: D005732:Gangliosides; D008075:Lipoproteins, HDL
keywords: HDL; apolipoproteins; atherosclerosis; cardiovascular disease; coronary artery disease; glycolipids; glycomics; proteomics