title: Alocasia denudata Engler treatment enhance open wound healing activities in Wistar rat's skin.
abstract: BACKGROUND
  A. denudata is traditionally used to treat various skin disorders, including wounds. It is widely used by the traditional healers as an effective wound treatment.
  
  
  OBJECTIVE
  This study was done to determine A. denudata treatment effects on open wound healing activities in Wistar rat's skin.
  
  
  METHODS
  120 Wistar rats (250-300 g) were divided into four main groups, 1.5% and 3% A. denudata stem juice treated group, 10% Solcoseryl® gel treated group as positive control and phosphate buffer saline (PBS) treated group as negative control. Six full thicknesses wounds (6mm) were induced bilaterally on the dorsal of the rat's skin. Rats were sacrificed on day 1, 3, 6, 10 and 14. The percentage of wound contraction, biochemical estimations, macroscopic observation and histological examinations were done to evaluate the wound healing activities.
  
  
  RESULTS
  Results showed wounds treated with A. denudata stem juice possess a significant higher rate of wound contraction (p<0.001), total protein concentration (p<0.05), hexosamine concentration (p<0.001) and uronic acid concentration (p<0.001). Moreover, cathepsin B (p<0.05) and hydroxyproline (p<0.05) level showed lower concentration in wounds treated with A. denudata stem juice. Histological observation of wounds treated with A. denudata stem juice displayed organized epithelial layer with dense and compact collagen fibers.
  
  
  CONCLUSIONS
  Both doses of A. denudata stem juice were found to enhance wound healing process. However, wounds treated with 3% A. denudata stem juice were reported to be more effective as a wound healing agent thus support its traditional usage.
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Mazlyzam Abdul
authors.initials: MA
authors.lastname: Latif
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Mohd Zulasyraf Mohd
authors.initials: MZ
authors.lastname: Zaki
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Tai May
authors.initials: TM
authors.lastname: Leng
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Noor Hidayah Abdul
authors.initials: NH
authors.lastname: Rahman
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
authors.forename: Siti Aisyah
authors.initials: SA
authors.lastname: Arshad
authors.affiliation: Programme of Biomedical Science, School of Diagnostic & Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. Electronic address: asmah0901@yahoo.com.
authors.forename: Asmah
authors.initials: A
authors.lastname: Hamid
mesh_terms: D031055:Alocasia; D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D003879:Dermatologic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D006595:Hexosamines / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D010940:Plant Proteins / Q000378:metabolism; D018547:Plant Stems; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014574:Uronic Acids / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D003879:Dermatologic Agents; D006595:Hexosamines; D010936:Plant Extracts; D010940:Plant Proteins; D014574:Uronic Acids; D002401:Cathepsin B; D006909:Hydroxyproline
keywords: Alocasia denudata Engler; Skin; Wound healing