title: A novel lumazine synthase molecule from Brucella significantly promotes the immune-stimulation effects of antigenic protein.
abstract: Brucella, an intracellular parasite that infects some livestock and humans, can damage or destroy the reproductive system of livestock. The syndrome is referred to as brucellosis and often occurs in pastoral areas; it is contagious from livestock to humans. In this study, the intact Brucella suis outer membrane protein 31 (omp31) gene was cloned, recombinantly expressed, and examined as a subunit vaccine candidate. The intact Brucella lumazine synthase (bls) gene was cloned and recombinantly expressed to study polymerization function in vitro. Non-reducing gel electrophoresis showed that rBs-BLS existed in different forms in vitro, including as a dimer and a pentamer. An enzyme-linked immunosorbent assay result showed that rOmp31 protein could induce production of an antibody in rabbits. However, the rOmp31-BLS fusion protein could elicit a much higher antibody titer in rabbits; this construct involved fusion of the Omp31 molecule with the BLS molecule. Our results indicate that Omp31 is involved in immune stimulation, while BLS has a polymerizing function based on rOmp31-BLS fusion protein immunogenicity. These data suggest that Omp31 is an ideal subunit vaccine candidate and that the BLS molecule is a favorable transport vector for antigenic proteins.
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia Autonomous Region, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Du
authors.affiliation: The Institute of Bioengineering and Technology, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia Autonomous Region, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002002:Brucella / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary; D056747:Immunomodulation; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; C044822:6,7-dimethyl-8-ribityllumazine synthase