title: TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells.
abstract: OBJECTIVE
  The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs).
  
  
  METHODS
  TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls.
  
  
  RESULTS
  In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation.
  
  
  CONCLUSIONS
  The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC. Electronic address: acolufreia@yahoo.com.tw.
authors.forename: Hsin-Chien
authors.initials: HC
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Cheng-Ping
authors.initials: CP
authors.lastname: Shih
authors.affiliation: Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Sheau-Huei
authors.initials: SH
authors.lastname: Chueh
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Shu-Fan
authors.initials: SF
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Hang-Kang
authors.initials: HK
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Lin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003051:Cochlea / Q000166:cytology*; D020868:Gene Silencing; D008808:Mice, Inbred CBA; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013136:Spiral Ganglion / Q000166:cytology*; D013234:Stem Cells / Q000502:physiology*; D050052:TRPC Cation Channels / Q000235:genetics / Q000502:physiology*
chemical_list: D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1
keywords: Canonical transient receptor potential channel; Cochlea; Proliferation; Spiral ganglion neuron; Stem/progenitor cell