title: A Peptide to Reduce Pulmonary Edema in a Rat Model of Lung Transplantation.
abstract: BACKGROUND
  Despite significant advances in organ preservation, surgical techniques and perioperative care, primary graft dysfunction is a serious medical problem in transplantation medicine in general and a specific problem in patients undergoing lung transplantation. As a result, patients develop lung edema, causing reduced tissue oxygenation capacity, reduced lung compliance and increased requirements for mechanical ventilatory support. Yet, there is no effective strategy available to protect the grafted organ from stress reactions induced by ischemia/reperfusion and by the surgical procedure itself.
  
  
  METHODS
  We assessed the effect of a cingulin-derived peptide, XIB13 or a random peptide in an established rat model of allogeneic lung transplantation. Donor lungs and recipients received therapeutic peptide at the time of transplantation and outcome was analyzed 100min and 28 days post grafting.
  
  
  RESULTS
  XIB13 improved blood oxygenation and reduced vascular leak 100min post grafting. Even after 28 days, lung edema was significantly reduced by XIB13 and lungs had reduced fibrotic or necrotic zones. Moreover, the induction of an allogeneic T cell response was delayed indicating a reduced antigen exchange between the donor and the host.
  
  
  CONCLUSIONS
  In summary, we provide a new tool to strengthen endothelial barrier function thereby improving outcomes in lung transplantation.
authors.affiliation: Department of Dermatology, Skin and Endothelium Research Division (SERD) Medical University of Vienna, Vienna, Austria.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Schossleitner
authors.affiliation: Department of Cardiac Surgery Medical University of Vienna, Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Habertheuer
authors.affiliation: Department of Dermatology, Skin and Endothelium Research Division (SERD) Medical University of Vienna, Vienna, Austria.
authors.forename: Richard
authors.initials: R
authors.lastname: Finsterwalder
authors.affiliation: Department of Dermatology, Skin and Endothelium Research Division (SERD) Medical University of Vienna, Vienna, Austria.
authors.forename: Heinz P
authors.initials: HP
authors.lastname: Friedl
authors.affiliation: Core Facility Imaging Medical University of Vienna, Vienna, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Rauscher
authors.affiliation: Core Facility Imaging Medical University of Vienna, Vienna, Austria.
authors.forename: Marion
authors.initials: M
authors.lastname: Gröger
authors.affiliation: Department of Cardiac Surgery Medical University of Vienna, Vienna, Austria.
authors.forename: Alfred
authors.initials: A
authors.lastname: Kocher
authors.affiliation: Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases Medical University of Vienna, Vienna, Austria.
authors.forename: Christine
authors.initials: C
authors.lastname: Wagner
authors.affiliation: Department of Dermatology, Division of Immunology, Allergy and Infectious Diseases Medical University of Vienna, Vienna, Austria.
authors.forename: Stephan N
authors.initials: SN
authors.lastname: Wagner
authors.affiliation: Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.
authors.forename: Gottfried
authors.initials: G
authors.lastname: Fischer
authors.affiliation: Department of Intensive Care & Laboratory of Experimental Intensive Care and Anesthesiology (L.E.I.C.A), Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Schultz
authors.affiliation: Department of Cardiac Surgery Medical University of Vienna, Vienna, Austria.
authors.forename: Dominik
authors.initials: D
authors.lastname: Wiedemann
authors.affiliation: Department of Dermatology, Skin and Endothelium Research Division (SERD) Medical University of Vienna, Vienna, Austria.
authors.forename: Peter
authors.initials: P
authors.lastname: Petzelbauer
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009926:Organ Preservation; D010446:Peptide Fragments / Q000494:pharmacology*; D055031:Primary Graft Dysfunction / Q000209:etiology / Q000517:prevention & control*; D011654:Pulmonary Edema / Q000209:etiology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014655:Vascular Resistance / Q000187:drug effects*; D014691:Ventilation
chemical_list: C061407:CGN protein, human; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010446:Peptide Fragments