title: [The effect of TLR4/MyD88/NF-κB signaling pathway on proliferation and apoptosis in human nasopharyngeal carcinoma 5-8F cells induced by LPS].
abstract: OBJECTIVE
  To evaluate the effects of NF-κB activation on the proliferation and apoptosis throughTLR4/MyD88 signaling pathway in human nasopharyngeal carcinoma (NPC) 5-8F cell lines.
  
  
  METHODS
  TLR4 induced by LPS is inhibited by PE anti-human. Real-Time Quantitative PCR and Western blot were employed to evaluate the efficacy of mRNA level and protein expression. The growth inhibition rate of 5-8F by Celecoxib was evaluated with MTT method. The cell cycle and apoptosis were measured with flow cytometric method (FCM).
  
  
  RESULTS
  By using the specific inhibitor, the protein and gene expression of NF-κB and MyD88 were both significantly lower than the control group (P<. 05). Meanwhile, the down-rugulation of NF-κB could inhibit proliferation of NPC 5-8F cells and promote their apoptosis (P<0. 05).
  
  
  CONCLUSIONS
  By inhibiting TLR4 / MyD88 signaling pathway, the expression of NF-κB in NPC 5-8F cells could decrease, then the cell proliferation was inhibited and cell apoptosis was induced. The results showed that TLR4 / MyD88 / NF-κB induced by LPS is an important pathway in the genesis and development of NPC. This study provides evidence for targeting research of NPC.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Li
authors.forename: Guangru
authors.initials: G
authors.lastname: Xie
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Zhangshen
authors.initials: Z
authors.lastname: Jiang
authors.forename: Zhensong
authors.initials: Z
authors.lastname: Yue
authors.forename: Zhanyu
authors.initials: Z
authors.lastname: Pan
mesh_terms: D017209:Apoptosis; D002277:Carcinoma; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4