title: In situ dsDNA-bevacizumab anticancer monoclonal antibody interaction electrochemical evaluation.
abstract: The interaction of the anticancer monoclonal antibody bevacizumab (BEVA) with double-stranded DNA (dsDNA) was studied by voltammetry and gel-electrophoresis in incubated samples and using the dsDNA-electrochemical biosensor. The voltammetric results revealed a decrease and disappearance of the dsDNA oxidation peaks with increasing incubation time, showing that BEVA binds to the dsDNA but no DNA oxidative damage was detected electrochemically. Non denaturing agarose gel-electrophoresis experiments were in agreement with the voltammetric results showing the formation of compact BEVA-dsDNA adduct. The dsDNA-electrochemical biosensor in incubated solutions showed that BEVA also undergoes structural modification upon binding dsDNA, and BEVA electroactive amino acid residues oxidation peaks were detected.
authors.affiliation: Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, 3004-535, Coimbra, Portugal.
authors.forename: Luciana I N
authors.initials: LI
authors.lastname: Tomé
authors.affiliation: Serviços Farmacêuticos, Centro Hospitalar e Universitário de Coimbra, EPE, 3000-075 Coimbra, Portugal.
authors.forename: Nuno V
authors.initials: NV
authors.lastname: Marques
authors.affiliation: Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, 3004-535, Coimbra, Portugal.
authors.forename: Victor C
authors.initials: VC
authors.lastname: Diculescu
authors.affiliation: Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, 3004-535, Coimbra, Portugal. Electronic address: brett@ci.uc.pt.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Oliveira-Brett
mesh_terms: D000068258:Bevacizumab / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D055664:Electrochemical Techniques / Q000379:methods*
chemical_list: D000068258:Bevacizumab; D004247:DNA
keywords: Bevacizumab; DNA damage; Glassy carbon; Interaction; Monoclonal antibody; Voltammetry; dsDNA-electrochemical biosensor