title: [ISOLATION AND IDENTIFICATION OF CARTILAGE PROGENITOR CELLS AND INFLUENCE OF INTERLEUKIN 1β ON ITS CHONDROGENESIS].
abstract: OBJECTIVE
  To isolate and identify the cartilage progenitor cells (CPCs) from normal cartilage, and to explore the influence of interleukin 1β (IL-1β) in different concentrations on its chondrogenesis.
  
  
  METHODS
  CPCs were isolated from normal cartilage of adult New Zealand white rabbit with the fibronectin adhesion assay; the cell phenotype was identified; and the cloning and differentiation of CPCs were observed. CPCs were incubated with H-DMEM in group A, with chondrogenic induced medium in group B, with chondrogenic induced medium+0.1 ng/mL IL-1β in group C and chondrogenic induced medium+1.0 ng/mL IL-1β in group D for 3 weeks. The histology, biochemistry, and real-time fluorescence quantitative PCR were performed to observe the effect of IL-1β on the chondrgenic differentiation.
  
  
  RESULTS
  The CPCs from normal cartilage expressed positively stem cell phenotype, which have similar ability of cloning and differentiation to stem cells. The cell pellets in groups C and D were significantly smaller than those in group B, and cell showed hypertrophic morphology change. There were more expressions of collagen type II and collagen type X in group B than in group A, in group B than in groups C and D, and in group C than group D with Safranin O staining. The biochemistry results showed that collagen type II content, glycosaminoglycan (GAG) content, and the ratio of GAG/DNA were significantly lower in groups C and D than in group B (P < 0.05), and in group D than in group C (P < 0.05); but the DNA content was significantly higher in groups C and D than in group B (P < 0.05), and no significant difference between groups C and D (P > 0.05). The real-time fluorescence quantitative PCR results showed that the relative mRNA expressions of collagen type II, collagen type X, and Sox-9 were significantly lower in groups C and D than in group B (P < 0.05), and in group D than in group C (P < 0.05), but the relative mRNA expressions of Runx-2 and matrix metalloproteinase 13 were significantly higher in groups C and D than in group B (P < 0.05), and in group D than in group C (P < 0.05).
  
  
  CONCLUSIONS
  There are CPCs having the character of stem cells in normal cartilage, and they have the capability of cloning and potential differentiation. IL-1β can inhibit the chondrogenesis of CPCs, and possibly promote the osteogenic differentiation.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Ding
authors.forename: Yiqiu
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jianchao
authors.initials: J
authors.lastname: Gui
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D024121:Collagen Type X / Q000235:genetics / Q000378:metabolism; D006025:Glycosaminoglycans; D053583:Interleukin-1beta / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D024043:Collagen Type II; D024121:Collagen Type X; D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D012333:RNA, Messenger