title: In vitro effect of sodium nitrite on platelet aggregation in human platelet rich plasma--preliminary report.
abstract: OBJECTIVE
  The role of nitrates and nitric oxide on platelet functions has obtained an increasing attention with respect to their potential effects on cardiovascular disorders. In this study we aimed to analyze the effect of sodium nitrite on platelet functions in human platelets.
  
  
  METHODS
  This in vitro study was designed to show the effect of sodium nitrite on platelet functions in seven healthy volunteers. Blood samples were centrifuged to prepare platelet rich plasma and platelet poor plasma. Platelet rich plasma was diluted with the platelet poor plasma to have a final count of 300,000 ± 25,000 platelets. Platelet rich plasma was incubated with six different increasing doses (from 10 μM to 5 mM) of sodium nitrite for 1 hour at 37°C. Then stimulating agents including collagen (3 μg ml-1), adenosine diphosphate (10 μM), and epinephrine (10 μM) were added to the cuvette. Changes in light transmission were observed for 10 minutes. In addition spontaneous aggregation were performed in control group with all aggregating agents separately.
  
  
  RESULTS
  Effect of sodium nitrite on agonist-induced platelet aggregation depends on the concentration of sodium nitrite. Compared with control group, agonist-induced platelet aggregations were significantly suppressed by sodium nitrite at the concentration of 5, 1.0 and 0.5 mM.
  
  
  CONCLUSIONS
  Our results suggested that sodium nitrite has inhibitory effects in vitro on platelet aggregation in a dose-dependent manner.
authors.affiliation: Department of Cardiovascular Surgery, Gulhane Military Academy of Medicine, Etlik, Ankara, Turkey. muratkadan@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Kadan
authors.forename: S
authors.initials: S
authors.lastname: Doğanci
authors.forename: V
authors.initials: V
authors.lastname: Yildirim
authors.forename: G
authors.initials: G
authors.lastname: Özgür
authors.forename: G
authors.initials: G
authors.lastname: Erol
authors.forename: K
authors.initials: K
authors.lastname: Karabacak
authors.forename: F
authors.initials: F
authors.lastname: Avcu
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects* / Q000502:physiology; D010979:Platelet Function Tests / Q000379:methods; D053657:Platelet-Rich Plasma / Q000187:drug effects* / Q000502:physiology; D012977:Sodium Nitrite / Q000494:pharmacology*
chemical_list: D000244:Adenosine Diphosphate; D012977:Sodium Nitrite