title: BODIPY/Nile-Red-Based Efficient FRET Pair: Selective Assay of Endoplasmic Reticulum Membrane Fluidity.
abstract: We synthesized a boron-dipyrromethene (BODIPY)/Nile Red hybrid probe capable of selectively recognizing fluidity changes in the endoplasmic reticulum (ER) membrane due to its preferential localization to the ER and strong energy transfer from BODIPY to the Nile Red moiety, emitting only in nonaqueous environments. ER membrane fluidity in HepG2 cells was markedly reduced by a cell model of metabolic syndrome.
authors.affiliation: Department of Chemistry, Korea University, Seoul, 136-701, Korea.|Key Laboratory of optoelectronic devices and systems of ministry of education and Guangdong province, College of optoelectronic engineering, Shenzhen University, Shenzhen, 518060, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701, Korea.
authors.forename: Youngjin
authors.initials: Y
authors.lastname: Wi
authors.affiliation: The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701, Korea.
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Yoon
authors.affiliation: Department of Chemistry, Korea University, Seoul, 136-701, Korea.
authors.forename: Peter
authors.initials: P
authors.lastname: Verwilst
authors.affiliation: Department of Chemistry, Korea University, Seoul, 136-701, Korea.
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Jang
authors.affiliation: The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701, Korea. tw1275@khu.ac.kr.
authors.forename: Tae Woo
authors.initials: TW
authors.lastname: Kim
authors.affiliation: The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701, Korea. kangch@khu.ac.kr.
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Korea University, Seoul, 136-701, Korea. jongskim@korea.ac.kr.
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008560:Membrane Fluidity; D024821:Metabolic Syndrome / Q000175:diagnosis; D061848:Optical Imaging; D010078:Oxazines / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010078:Oxazines; C044808:nile red
keywords: FRET; endoplasmic reticulum; fluorescence; membrane fluidity; metabolic syndrome