title: A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae.
abstract: Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3'-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.
authors.affiliation: a Department of Applied Life Science , United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsujii
authors.affiliation: b Faculty of Agriculture, Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okuda
authors.affiliation: c Department of Applied Biological Chemistry , Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Kazutomo
authors.initials: K
authors.lastname: Ishi
authors.affiliation: c Department of Applied Biological Chemistry , Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Madokoro
authors.affiliation: a Department of Applied Life Science , United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology , Fuchu , Japan.|b Faculty of Agriculture, Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.|c Department of Applied Biological Chemistry , Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: a Department of Applied Life Science , United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology , Fuchu , Japan.|b Faculty of Agriculture, Department of Applied Biological Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.|c Department of Applied Biological Chemistry , Graduate School of Agriculture, Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Yamagata
mesh_terms: D001236:Aspergillus oryzae / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005091:Exons; D020224:Expressed Sequence Tags; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013172:Spores, Fungal / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: Aspergillus oryzae; CSLNR; long natural antisense RNA