title: Combinatorial effects of genistein and sex-steroids on the level of cystic fibrosis transmembrane regulator (CFTR), adenylate cyclase (AC) and cAMP in the cervix of ovariectomised rats.
abstract: The combinatorial effects of genistein and estrogen (E) or estrogen plus progesterone (E+P) on CFTR, AC and cAMP levels in cervix were investigated. Ovariectomised adult female rats received 50 or 100mg/kg/day genistein with E or E followed by E+P [E+(E+P)] for seven consecutive days. Cervixes were harvested and analyzed for CFTR mRNA levels by Real-time PCR. Distribution of AC and CFTR proteins in endocervix were observed by immunohistochemistry. Levels of cAMP were measured by enzyme-immunoassay. Molecular docking predicted interaction between genistein and AC. Our results indicate that levels of CFTR, AC and cAMP in cervix of rats receiving genistein plus E were higher than E-only treatment (p<0.05) while genistein plus [E+(E+P)] were higher than E+(E+P)-only treatment (p<0.05). In conclusions, increased levels of CFTR, AC and cAMP in cervix of E and E+(E+P)-treated rats by genistein could affect the cervical secretory function which could influence the female reproductive processes.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia. Electronic address: naguib.salleh@yahoo.com.my.
authors.forename: Naguib
authors.initials: N
authors.lastname: Salleh
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia.
authors.forename: Nurain
authors.initials: N
authors.lastname: Ismail
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia.
authors.forename: Sekaran
authors.initials: S
authors.lastname: Muniandy
authors.affiliation: Department of Biomedical Informatics, Asia University, Taichung 41354, Taiwan.
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Korla
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Lembah Pantai, Kuala Lumpur, Malaysia.
authors.forename: Nelli
authors.initials: N
authors.lastname: Giribabu
mesh_terms: D000262:Adenylyl Cyclases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002584:Cervix Uteri / Q000187:drug effects* / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D019833:Genistein / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D062105:Molecular Docking Simulation; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D011485:Protein Binding; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D013997:Time Factors; D015854:Up-Regulation
chemical_list: C579654:CFTR protein, rat; D004967:Estrogens; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D011374:Progesterone; D019833:Genistein; D000242:Cyclic AMP; D000262:Adenylyl Cyclases
keywords: AC; CFTR; Cervix; Genistein; Sex-steroids; cAMP