title: GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions.
abstract: There are numerous cell types with scarcely understood functions, whose interactions with the immune system are not well characterized. To facilitate their study, we generated a mouse bearing enhanced green fluorescent protein (EGFP)-specific CD8+T cells. Transfer of the T cells into EGFP reporter animals can be used to kill EGFP-expressing cells, allowing selective depletion of desired cell types, or to interrogate T-cell interactions with specific populations. Using this system, we eliminate a rare EGFP-expressing cell type in the heart and demonstrate its role in cardiac function. We also show that naive T cells are recruited into the mouse brain by antigen-expressing microglia, providing evidence of an immune surveillance pathway in the central nervous system. The just EGFP death-inducing (Jedi) T cells enable visualization of a T-cell antigen. They also make it possible to utilize hundreds of existing EGFP-expressing mice, tumors, pathogens and other tools, to study T-cell interactions with many different cell types, to model disease states and to determine the functions of poorly characterized cell populations.
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Judith
authors.initials: J
authors.lastname: Agudo
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Albert
authors.initials: A
authors.lastname: Ruzo
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Park
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Sweeney
authors.affiliation: Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kana
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The New York Stem Cell Foundation Research Institute, New York, New York, USA.
authors.forename: Dieter
authors.initials: D
authors.lastname: Egli
authors.affiliation: Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.|Mount Sinai Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.|Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.|Mount Sinai Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.|Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.|Diabetes Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Brown