title: Macrophage activation induced by Brucella DNA suppresses bacterial intracellular replication via enhancing NO production.
abstract: Brucella DNA can be sensed by TLR9 on endosomal membrane and by cytosolic AIM2-inflammasome to induce proinflammatory cytokine production that contributes to partially activate innate immunity. Additionally, Brucella DNA has been identified to be able to act as a major bacterial component to induce type I IFN. However, the role of Brucella DNA in Brucella intracellular growth remains unknown. Here, we showed that stimulation with Brucella DNA promote macrophage activation in TLR9-dependent manner. Activated macrophages can suppresses wild type Brucella intracellular replication at early stage of infection via enhancing NO production. We also reported that activated macrophage promotes bactericidal function of macrophages infected with VirB-deficient Brucella at the early or late stage of infection. This study uncovers a novel function of Brucella DNA, which can help us further elucidate the mechanism of Brucella intracellular survival.
authors.affiliation: Central Laboratory, The Second Hospital of Jilin University, Changchun 130041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
authors.forename: Wanchun
authors.initials: W
authors.lastname: Sun
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China. Electronic address: Qishengpeng@yahoo.com.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002002:Brucella / Q000254:growth & development* / Q000276:immunology*; D004269:DNA, Bacterial / Q000276:immunology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism*; D051217:Toll-Like Receptor 9 / Q000819:agonists / Q000172:deficiency
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D009569:Nitric Oxide
keywords: Activation; Brucella DNA; Macrophage; NO