title: A new method for separation and determination of Cr(III) and Cr(VI) in water samples by high-performance liquid chromatography based on anion exchange stationary phase of ionic liquid modified silica.
abstract: In this work, N-methylimidazolium-chloride ionic liquid functionalized silica was prepared and used as an anion-exchange stationary phase for separation of chromium species by high-performance liquid chromatography (HPLC) with UV detection at 200 nm. The Cr(VI) as HCr2O7(-) and chelated Cr(III) with potassium hydrogen phthalate (PHP) as Cr(PHP)2 (-) was retained on the prepared column and separated using a mobile phase composed of 5% methanol in 25 mM phosphate buffer at pH 6.5. Several variables affecting the chelation/separation steps were modeled by response surface methodology (RSM) using Box-Behnken (BBD) design. The significance of the independent variables and their interactions were tested by the analysis of variances (ANOVA) with 95% confidence limit. Under the optimized conditions, the Cr(III) and Cr(VI) anionic species were well separated with a single peak for each Cr species at retention times of 2.3 and 4.3 min, respectively. The relationship between the peak area and concentration was linear in the range of 0.025-30 for Cr(III) and 0.5-20 mg L(-1) for Cr(VI) with detection limits of 0.010 and 0.210 mg L(-1) for Cr(III) and Cr(VI), respectively. The proposed method was validated by simultaneous separation and determination of the Cr species in tap and underground water samples without impose to any pretreatment.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Birjand, Birjand, South Khorasan, Iran. ssadeghi@birjand.ac.ir.
authors.forename: Susan
authors.initials: S
authors.lastname: Sadeghi
authors.affiliation: Department of Chemistry, Faculty of Science, University of Birjand, Birjand, South Khorasan, Iran.
authors.forename: Ali Zeraatkar
authors.initials: AZ
authors.lastname: Moghaddam
mesh_terms: D000838:Anions / Q000032:analysis; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid; D002857:Chromium / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D052578:Ionic Liquids / Q000737:chemistry; D007477:Ions / Q000032:analysis; D012822:Silicon Dioxide / Q000032:analysis / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000838:Anions; D002614:Chelating Agents; D052578:Ionic Liquids; D007477:Ions; D014874:Water Pollutants, Chemical; D002857:Chromium; C074702:chromium hexavalent ion; D012822:Silicon Dioxide
keywords: Anion-exchange stationary phase; Chromium; Ionic liquids; Response surface methodology; Speciation