title: Osteogenic Differentiation Gene Expression Profiling of hMSCs on Hydroxyapatite and Mineralized Collagen.
abstract: In this study, human mesenchymal stem cells (hMSCs) were cultured on the hydroxyapatite (HA) and mineralized collagen (MC), and their proliferation, adhesion, and differentiation, especially the molecular mechanisms on gene level, were investigated. Proliferation and morphological responses of hMSCs and their osteogenic differentiation were detected by quantitative detection of alkaline phosphatase. Gene expression profilings were examined by microarrays, and the gene expression data were studied through gene ontology terms and pathway analyses. The results showed that MC promoted cell proliferation and osteogenic differentiation of hMSCs. Microarray analysis showed that MC was conducive to express osteogenesis-related genes, such as BMP-2, COL1A1, and CTSK, and stimulate osteogenic differentiation, such as osteoblast differentiation pathway and skeletal system development pathway.
authors.affiliation: 1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .|2 College of Life Sciences, Zhejiang Sci-Tech University , Hangzhou, China .
authors.forename: Su-Ju
authors.initials: SJ
authors.lastname: Xu
authors.affiliation: 1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .
authors.forename: Zhi-Ye
authors.initials: ZY
authors.lastname: Qiu
authors.affiliation: 1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .|3 School of Engineering and Technology, China University of Geosciences , Beijing, China .
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Wu
authors.affiliation: 2 College of Life Sciences, Zhejiang Sci-Tech University , Hangzhou, China .
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Kong
authors.affiliation: 4 Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College , Beijing, China .
authors.forename: Xi-Sheng
authors.initials: XS
authors.lastname: Weng
authors.affiliation: 1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
authors.affiliation: 1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism*; D017886:Durapatite / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects*
chemical_list: D000943:Antigens, Differentiation; D003094:Collagen; D017886:Durapatite