title: Protective effects of thymoquinone against apoptosis and oxidative stress by arsenic in rat kidney.
abstract: We aimed to investigate the protective role of thymoquinone (TQ) by targeting its antiapoptotic and antioxidant properties against kidney damage induced by arsenic in rats. We have used the 24 male Sprague-Dawley rats. Rats were divided into three groups. Physiological serum in 10 mL/kg dose as intragastric was given to the control group. Sodium arsenite (10 mg/kg, intragastric by gavage for fifteen days) was given to the arsenic group. Sodium arsenite (10 mg/kg, intragastric by gavage for fifteen days) and TQ (10 mg/kg, intragastric by gavage for 15 days) was given to the arsenic + TQ group. After 15 days, the animals' kidneys were taken theirs, then we have performed histological and apoptotic assessment. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzyme activities and malondialdehyde (MDA) levels have examined as the oxidative stress parameters. We have determined the levels of arsenic. Increased renal injury and apoptotic cells have been detected in the arsenic group. Degenerative changes in the arsenic + TQ group were diminished. Although the MDA levels were augmented in the arsenic group, SOD, CAT and GSH-Px enzyme activities were lessened than the other groups. Our findings suggest that TQ may impede the oxidative stress, the cells have been damaged and also the generation of apoptotic cells arisen from arsenic. TQ plays a protective role against arsenic-induced toxicity in kidney and may potentially be used as a remedial agent.
authors.affiliation: a Department of Physiology , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Umit
authors.initials: U
authors.lastname: Sener
authors.affiliation: b Department of Anatomy , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Ramazan
authors.initials: R
authors.lastname: Uygur
authors.affiliation: c Department of Histology and Embryology , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Cevat
authors.initials: C
authors.lastname: Aktas
authors.affiliation: a Department of Physiology , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Emine
authors.initials: E
authors.lastname: Uygur
authors.affiliation: c Department of Histology and Embryology , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Mustafa
authors.initials: M
authors.lastname: Erboga
authors.affiliation: d Department of Biochemistry , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey , and.
authors.forename: Gulseren
authors.initials: G
authors.lastname: Balkas
authors.affiliation: b Department of Anatomy , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Veli
authors.initials: V
authors.lastname: Caglar
authors.affiliation: e Department of Forensic Medicine , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Kumral
authors.affiliation: d Department of Biochemistry , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey , and.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gurel
authors.affiliation: a Department of Physiology , Faculty of Medicine, Namik Kemal University , Tekirdag , Turkey .
authors.forename: Hasan
authors.initials: H
authors.lastname: Erdogan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D001151:Arsenic / Q000378:metabolism; D020261:Arsenic Poisoning / Q000150:complications* / Q000201:enzymology / Q000473:pathology; D016227:Benzoquinones / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D007668:Kidney / Q000201:enzymology / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D016227:Benzoquinones; D008315:Malondialdehyde; D001151:Arsenic; C003466:thymoquinone
keywords: Arsenic; apoptosis; kidney; oxidative stress; thymoquinone