title: Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units.
abstract: Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids 218-228), respectively, within the dorsal striatum. After nicotine administration, there was a positive shift in correlation of mass/charge peak expression levels with substance P and proenkephalin A (218-228). Our exploratory analyses demonstrate the technical capacity of MALDI-IMS for comprehensive identification of peptidomic regulation patterns along histochemically distinguishable striatal projection pathways.
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Akitoyo
authors.initials: A
authors.lastname: Hishimoto
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nomaru
authors.affiliation: Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Kenny
authors.initials: K
authors.lastname: Ye
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Akira
authors.initials: A
authors.lastname: Nishi
authors.affiliation: Laboratory for Macromolecular Analysis & Proteomics, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Jihyeon
authors.initials: J
authors.lastname: Lim
authors.affiliation: Laboratory for Macromolecular Analysis & Proteomics, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Jennifer T
authors.initials: JT
authors.lastname: Aguilan
authors.affiliation: Laboratory for Macromolecular Analysis & Proteomics, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Edward
authors.initials: E
authors.lastname: Nieves
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Gina
authors.initials: G
authors.lastname: Kang
authors.affiliation: Laboratory for Macromolecular Analysis & Proteomics, Albert Einstein College of Medicine, Bronx, New York.
authors.forename: Ruth Hogue
authors.initials: RH
authors.lastname: Angeletti
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, New York; Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Albert Einstein College of Medicine, Bronx, New York; Department of Genetics, Albert Einstein College of Medicine, Bronx, New York. Electronic address: noboru.hiroi@einstein.yu.edu.
authors.forename: Noboru
authors.initials: N
authors.lastname: Hiroi
mesh_terms: D000818:Animals; D004745:Enkephalins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004745:Enkephalins; D009479:Neuropeptides; D011498:Protein Precursors; C029992:proenkephalin
keywords: Caudate-putamen; In situ mass spectrometry; Nicotine addiction; Peptides; Peptidomics; Striatum