title: Nanoparticle-assisted laser desorption/ionization using sinapic acid-modified iron oxide nanoparticles for mass spectrometry analysis.
abstract: Iron oxide-based nanoparticles (NP) were covalently modified with sinapic acid (SA) through a condensation reaction to assist the ionization of both large and small molecules. The morphology of SA-modified NPs (SA-NP) was characterized by transmission electron microscopy (TEM), and the modification of the NP surface with SA was confirmed using ultraviolet (UV) and infrared (IR) spectroscopy. The number of SA molecules was estimated to be 6 per NP. SA-NP-assisted laser desorption/ionization was carried out on small molecules, such as pesticides and plant hormones, and large molecules, such as peptides and proteins. A peptide fragment from degraded proteins was detected more efficiently compared with conventional methods.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
authors.forename: Hanaka
authors.initials: H
authors.lastname: Komori
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
authors.forename: Riho
authors.initials: R
authors.lastname: Hashizaki
authors.affiliation: Center for Nano Materials and Technology, Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi-shi, Ishikawa 923-1292, Japan.
authors.forename: Issey
authors.initials: I
authors.lastname: Osaka
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Hibi
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
authors.forename: Hajime
authors.initials: H
authors.lastname: Katano
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Eiheiji, Fukui 910-1195, Japan. staira@fpu.ac.jp.
authors.forename: Shu
authors.initials: S
authors.lastname: Taira
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods; D003373:Coumaric Acids / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D013058:Mass Spectrometry; D053768:Metal Nanoparticles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003373:Coumaric Acids; D005290:Ferric Compounds; C000499:ferric oxide; C073734:sinapinic acid