title: Inflammation-induced oxidative stress in breast cancer patients.
abstract: Inflammation induced by cytokines has been linked to increased production of reactive oxygen species and breast cancer development. The aim of this study was to evaluate the influence COX-2, IL-1β, IL-8, and TNF-α gene expressions on DNA damage, and investigate a possible link between these factors with neoplastic process. The mRNA expression was measured by real-time PCR, and the DNA damage was analyzed by single-cell gel electrophoresis (comet assay). Our data indicated a significant increase on inflammatory gene expression in tumor tissues compared with normal tissue, and it was also associated with undifferentiated grade patients. Moreover, the results showed that the higher levels of DNA damage were observed among tumor tissue samples. Taken together, the findings presented in this study highlight the relevance of inflammation-induced oxidative stress in breast carcinogenesis.
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil.
authors.forename: Aline Tengan
authors.initials: AT
authors.lastname: Roque
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil.
authors.forename: Rafael Zuppardo
authors.initials: RZ
authors.lastname: Gambeloni
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil.
authors.forename: Simone
authors.initials: S
authors.lastname: Felitti
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil. marcelo.ribeiro@usf.edu.br.|Programa de Pós Graduação em Genética e Biologia Molecular, State University of Campinas, Campinas, SP, 13083-970, Brazil. marcelo.ribeiro@usf.edu.br.
authors.forename: Marcelo Lima
authors.initials: ML
authors.lastname: Ribeiro
authors.affiliation: Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Av. São Francisco de Assis, 218. Jd. São José, Bragança Paulista, SP, Brazil. ju_carvalho_s@hotmail.com.|Programa de Pós Graduação em Genética e Biologia Molecular, State University of Campinas, Campinas, SP, 13083-970, Brazil. ju_carvalho_s@hotmail.com.
authors.forename: Juliana Carvalho
authors.initials: JC
authors.lastname: Santos
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism*; D020552:Comet Assay; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D016207:Cytokines; D017382:Reactive Oxygen Species
keywords: Breast cancer; Comet assay; DNA damage; Gene expression; Inflammation