title: Production of viable trout offspring derived from frozen whole fish.
abstract: Long-term preservation of fish fertility is essential for the conservation of endangered fishes. However, cryopreservation techniques for fish oocytes and embryos have not yet been developed. In the present study, functional eggs and sperm were derived from whole rainbow trout that had been frozen in a freezer and stored without the aid of exogenous cryoprotectants. Type A spermatogonia retrieved from frozen-thawed whole trout remained viable after freezing duration up to 1,113 days. Long-term-frozen trout spermatogonia that were intraperitoneally transplanted into triploid salmon hatchlings migrated toward the recipient gonads, where they were incorporated, and proliferated rapidly. Although all triploid recipients that did not undergo transplantation were functionally sterile, 2 of 12 female recipients and 4 of 13 male recipients reached sexual maturity. Eggs and sperm obtained from the salmon recipients were capable of producing donor-derived trout offspring. This methodology is thus a convenient emergency tool for the preservation of endangered fishes.
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.|Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 404-708, Korea.
authors.forename: Seungki
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Seki
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Katayama
authors.affiliation: Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
authors.forename: Goro
authors.initials: G
authors.lastname: Yoshizaki
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology; D005260:Female; D005298:Fertility / Q000187:drug effects / Q000502:physiology*; D008297:Male; D017686:Oncorhynchus mykiss / Q000502:physiology*; D009865:Oocytes / Q000502:physiology; D013093:Spermatogonia / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents