title: [Significance of Th1/Th17 Imbalance in Mice with Acute Graft-versus-Host Disease].
abstract: OBJECTIVE
  To investigate the effects of Th1/Th17 cell imbalance on the pathogenesis of acute graft-versus-host disease (GVHD) in mice.
  
  
  METHODS
  In a murine GVHD model of C57BL/6 (H-2(b)), a low dose of halofuginone (HF) was applied for treating the recipients in order to result in Th1/Th17 imbalance. Rechipient mice were divided into GVHD group (without HF intervention) and GVHD plus HF group (treated by HF). The recipients were monitored for survival rate, clinical scores of acute GVHD, contents of circulatory Th1 and Th17 cells, Th1/Th17 ratio and serum level of IFN-γ and IL-17A. Expression levels of IFN-γ and IL-17A in target organs were analyzed by using real-time PCR, and the target organs were delivered for histological examinations.
  
  
  RESULTS
  Recipients treated with HF showed that all the mortality, circulatory Th1/Th17 ratio and clinical score were higher than those in the mice without HF intervention (P < 0.05). Circulatory Th1/Th17 ratio positively correlates with clinical score (P < 0.001). HF administration reduces the expression level of intestinal IL-17A and increases intrahepatic and intestinal IFN-γ level (P < 0.05), HF treatment aggravates GVHD in liver and small intestine with augmented hepatic and intestinal inflammation.
  
  
  CONCLUSIONS
  Th1/Th17 imbalance contributes to the pathogenesis of acute GVHD.
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Bian
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Xia
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Song
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Ling-Yu
authors.initials: LY
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail: kailinxu_xzmc@126.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010880:Piperidines; D052999:Quinazolinones; D018417:Th1 Cells / Q000166:cytology*; D058504:Th17 Cells / Q000166:cytology*
chemical_list: C098680:Il17a protein, mouse; D020381:Interleukin-17; D010880:Piperidines; D052999:Quinazolinones; D007371:Interferon-gamma; C010176:halofuginone