title: NUP50 is necessary for the survival of primordial germ cells in mouse embryos.
abstract: Nucleoporin 50 kDa (NUP50), a component of the nuclear pore complex, is highly expressed in male germ cells, but its role in germ cells is largely unknown. In this study, we analyzed the expression and function of NUP50 during the embryonic development of germ cells using NUP50-deficient mice. NUP50 was expressed in germ cells of both sexes at embryonic day 15.5 (E15.5), E13.5, and E12.5. In addition, NUP50 expression was also detected in primordial germ cells (PGCs) migrating into the genital ridges at E9.5. The gonads of Nup50-/- embryos of both sexes contained few PGCs at both E11.5 and E12.5 and no developing germ cells at E15.5. The migratory PGCs in Nup50-/- embryos at E9.5 showed increased apoptosis but a normal rate of proliferation, resulting in the progressive loss of germ cells at later stages. Taken together, these results suggest that NUP50 plays an essential role in the survival of PGCs during embryonic development.
authors.affiliation: School of Biological Sciences and TechnologyKorea Basic Science Institute Gwangju CenterChonnam National University, Gwangju 500-757, Republic of KoreaDivisions of Clinical Research and Human BiologyFred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA School of Biological Sciences and TechnologyKorea Basic Science Institute Gwangju CenterChonnam National University, Gwangju 500-757, Republic of KoreaDivisions of Clinical Research and Human BiologyFred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: Eunsook
authors.initials: E
authors.lastname: Park
authors.affiliation: School of Biological Sciences and TechnologyKorea Basic Science Institute Gwangju CenterChonnam National University, Gwangju 500-757, Republic of KoreaDivisions of Clinical Research and Human BiologyFred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: Bobae
authors.initials: B
authors.lastname: Lee
authors.affiliation: School of Biological Sciences and TechnologyKorea Basic Science Institute Gwangju CenterChonnam National University, Gwangju 500-757, Republic of KoreaDivisions of Clinical Research and Human BiologyFred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Clurman
authors.affiliation: School of Biological Sciences and TechnologyKorea Basic Science Institute Gwangju CenterChonnam National University, Gwangju 500-757, Republic of KoreaDivisions of Clinical Research and Human BiologyFred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA klee@chonnam.ac.kr.
authors.forename: Keesook
authors.initials: K
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004622:Embryo, Mammalian / Q000166:cytology; D047108:Embryonic Development; D000066473:Embryonic Germ Cells / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006066:Gonads / Q000737:chemistry / Q000196:embryology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D028861:Nuclear Pore Complex Proteins / Q000032:analysis / Q000172:deficiency* / Q000502:physiology*
chemical_list: D028861:Nuclear Pore Complex Proteins; C105412:Nup50 protein, mouse