title: Rapidly responsive and highly selective fluorescent probe for sulfite detection in real samples and living cells.
abstract: Sulfites (HSO3(-) or SO3(-)) have very significant toxicity in the environment and in the system. However, developing specific identification of sulfite probes is still very important. In this paper, a highly selective colorimetric and fluorescent probe (HHC) was synthesized to detect HSO3(-) in real samples and living cells. Sensing performance and preponderance are listed as follows. First, probe HHC showed remarkable selectivity for HSO3(-) over varieties of other species, including cysteine, glutathione, S(2-), CN(-), and reactive oxygen species, mainly because of the introduction of the electron-poor C=C double bond for HSO3(-). Second, probe HHC has great molar absorptivity, allowing it to act as a visual detection of probe for HSO3(-). Third, the fluorescence intensities of HHC linearly correlate with the concentration of HSO3(-), with a detection limit of 6.8 nm. Finally, our proposed probe can be applied to the visually determination of trace HSO3(-) in real samples and living HeLa cells with high precision. We hope that our proposed probe will greatly benefit biological sciences when biological researchers survey the role of HSO3(-) in biological systems.
authors.affiliation: Department of Forensic Chemistry, National Police University of China, Shenyang 110854, China. Electronic address: lhd870821@163.com.
authors.forename: Hongda
authors.initials: H
authors.lastname: Li
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D002470:Cell Survival; D060766:Drinking Water / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D015394:Molecular Structure; D013447:Sulfites / Q000032:analysis*; D014407:Tumor Cells, Cultured; D014920:Wine / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D060766:Drinking Water; D005456:Fluorescent Dyes; D013447:Sulfites
keywords: Biomaging; Bisulfite detection; Fluorescent probe; Quantification; Real samples