title: Role of malate transporter in lipid accumulation of oleaginous fungus Mucor circinelloides.
abstract: Fatty acid biosynthesis in oleaginous fungi requires the supply of reducing power, NADPH, and the precursor of fatty acids, acetyl-CoA, which is generated in the cytosol being produced by ATP: citrate lyase which requires citrate to be, transported from the mitochondrion by the citrate/malate/pyruvate transporter. This transporter, which is within the mitochondrial membrane, transports cytosolic malate into the mitochondrion in exchange for mitochondrial citrate moving into the cytosol (Fig. 1). The role of malate transporter in lipid accumulation in oleaginous fungi is not fully understood, however. Therefore, the expression level of the mt gene, coding for a malate transporter, was manipulated in the oleaginous fungus Mucor circinelloides to analyze its effect on lipid accumulation. The results showed that mt overexpression increased the lipid content for about 70 % (from 13 to 22 % dry cell weight, CDW), whereas the lipid content in mt knockout mutant decreased about 27 % (from 13 to 9.5 % CDW) compared with the control strain. Furthermore, the extracellular malate concentration was decreased in the mt overexpressing strain and increased in the mt knockout strain compared with the wild-type strain. This work suggests that the malate transporter plays an important role in regulating lipid accumulation in oleaginous fungus M. circinelloides.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Departamento de Genética y Microbiología (Unidad asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, 30071, Murcia, Spain.
authors.forename: José T
authors.initials: JT
authors.lastname: Cánovas-Márquez
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People's Republic of China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People's Republic of China.
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China.|Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Departamento de Genética y Microbiología (Unidad asociada al IQFR-CSIC), Facultad de Biología, Universidad de Murcia, 30071, Murcia, Spain. vgarre@um.es.
authors.forename: Victoriano
authors.initials: V
authors.lastname: Garre
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, 214122, People's Republic of China. ysong@jiangnan.edu.cn.
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Biological Sciences, University of Hull, Hull, UK.
authors.forename: Colin
authors.initials: C
authors.lastname: Ratledge
mesh_terms: D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D050356:Lipid Metabolism; D008293:Malates / Q000378:metabolism*; D009089:Mucor / Q000235:genetics / Q000378:metabolism*; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008293:Malates; D027361:Organic Anion Transporters
keywords: Citrate/malate/pyruvate shuttle; Lipid content; Malate concentration; Malate transporter; Mucor circinelloides