title: Recombinant Mitochondrial Manganese Containing Superoxide Dismutase Protects Against Ochratoxin A-Induced Nephrotoxicity.
abstract: Ochratoxin A (OTA) is a natural mycotoxin, involved in the development of important human and animal diseases. In this work we have studied the role of oxidative stress in the development of OTA nephrotoxicity and the effect of a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) to prevent kidney damage induced by OTA. Blood pressure, glomerular filtration rate and renal histology were analyzed in control rats and in OTA treated rats. In addition, lipid peroxidation, catalase and superoxide dismutase productions were measured. Our data showed that animals treated with OTA presented hypertension and reduction of glomerular filtration rate (GFR). These effects are most probably related to an increase in the reactive oxygen species (ROS) productions. In fact, we have shown that treatment with rMnSOD restored the levels of blood pressure and GFR simultaneously. Moreover, we have noted that OTA induced alteration on glomerular and tubular degeneration and interstitial infiltrates and that use of rMnSOD combined with OTA prevent this renal histological damage confirming the potential therapeutic role in the treatment of rMnSOD OTA nephrotoxicity.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ciarcia
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Damiano
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Squillacioti
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Mirabella
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Ugo
authors.initials: U
authors.lastname: Pagnini
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Florio
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Lorella
authors.initials: L
authors.lastname: Severino
authors.affiliation: Department of Nephrology, Second University of Naples, Naples, Italy.
authors.forename: Giovambattista
authors.initials: G
authors.lastname: Capasso
authors.affiliation: Molecular Biology and Viral Oncology Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, "Fondazione G. Pascale", IRCCS, Naples, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Borrelli
authors.affiliation: Leadhexa Biotechnologies Inc., San Francisco, California.
authors.forename: Aldo
authors.initials: A
authors.lastname: Mancini
authors.affiliation: Sbarro Institute for Cancer Research and Molecular Medicine, Center of Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania.
authors.forename: Silvia
authors.initials: S
authors.lastname: Boffo
authors.affiliation: Sbarro Institute for Cancer Research and Molecular Medicine, Center of Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Romano
authors.affiliation: Sbarro Institute for Cancer Research and Molecular Medicine, Center of Biotechnology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania.|Department of Medicine, Surgery and Neuroscience, University of Siena, Siena, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Giordano
authors.affiliation: Department of Veterinary Medicine and Animal Productions, University of Naples "Federico II", 80137 Naples, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Florio
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007674:Kidney Diseases / Q000139:chemically induced / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008345:Manganese / Q000737:chemistry*; D009793:Ochratoxins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D009793:Ochratoxins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; C025589:ochratoxin A; D008345:Manganese; D013482:Superoxide Dismutase
keywords: NEPHROTOXICITY; OCHRATOXIN A; OXIDATIVE STRESS; rMnSOD