title: Dynamic nano-imaging of label-free living cells using electron beam excitation-assisted optical microscope.
abstract: Optical microscopes are effective tools for cellular function analysis because biological cells can be observed non-destructively and non-invasively in the living state in either water or atmosphere condition. Label-free optical imaging technique such as phase-contrast microscopy has been analysed many cellular functions, and it is essential technology for bioscience field. However, the diffraction limit of light makes it is difficult to image nano-structures in a label-free living cell, for example the endoplasmic reticulum, the Golgi body and the localization of proteins. Here we demonstrate the dynamic imaging of a label-free cell with high spatial resolution by using an electron beam excitation-assisted optical (EXA) microscope. We observed the dynamic movement of the nucleus and nano-scale granules in living cells with better than 100 nm spatial resolution and a signal-to-noise ratio (SNR) around 10. Our results contribute to the development of cellular function analysis and open up new bioscience applications.
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu 432-8561, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukuta
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu 432-8561, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kanamori
authors.affiliation: Institute of NanoScience Design, Osaka University, 1-3, Machikaneyamacho, Toyonaka 560-0043, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Furukawa
authors.affiliation: Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu 432-8561, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nawa
authors.affiliation: Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu 432-8561, Japan.|CREST, Japan Science and Technology Agency, 4-1-8, Honmachi, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Inami
authors.affiliation: CREST, Japan Science and Technology Agency, 4-1-8, Honmachi, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.affiliation: Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu 432-8561, Japan.|CREST, Japan Science and Technology Agency, 4-1-8, Honmachi, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawata
authors.affiliation: CREST, Japan Science and Technology Agency, 4-1-8, Honmachi, Kawaguchi, Saitama 332-0012, Japan.|Photon Medical Research Center, Hamamatsu University School of Medicine, Hondayama, Higashi, Hamamatsu.
authors.forename: Susumu
authors.initials: S
authors.lastname: Terakawa
mesh_terms: D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D004583:Electrons; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D008858:Microscopy, Phase-Contrast; D010316:Particle Size; D059629:Signal-To-Noise Ratio; D017640:Silicates / Q000737:chemistry; D059008:Time-Lapse Imaging; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D017640:Silicates; D017967:Zinc Compounds; D006046:Gold; C029500:zinc silicate