title: Temporal Dynamics of Acute Stress-Induced Dendritic Remodeling in Medial Prefrontal Cortex and the Protective Effect of Desipramine.
abstract: Stressful events are associated with increased risk of mood disorders. Volumetric reductions have been reported in brain areas critical for the stress response, such as medial prefrontal cortex (mPFC), and dendritic remodeling has been proposed as an underlying factor. Here, we investigated the time-dependent effects of acute stress on dendritic remodeling within the prelimbic (PL) region of the PFC, and whether treatment with the antidepressant desipramine (DMI) may interfere. Rodents were subjected to foot-shock stress: dendritic length and spine density were analyzed 1 day, 7 days, and 14 days after stress. Acute stress produced increased spine density and decreased cofilin phosphorylation at 1 day, paralleled with dendritic retraction. An overall shift in spine population was observed at 1 day, resulting in a stress-induced increase in small spines. Significant atrophy of apical dendrites was observed at 1 day, which was prevented by chronic DMI, and at 14 days after stress exposure. Chronic DMI resulted in dendritic elaboration at 7 days but did not prevent the effects of FS-stress. Collectively, these data demonstrate that 1) acute stressors may induce rapid and sustained changes of PL neurons; and 2) chronic DMI may protect neurons from rapid stress-induced synaptic changes.
authors.affiliation: Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus C 8000, Denmark.|Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov 8240, Denmark.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Nava
authors.affiliation: Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov 8240, Denmark.|Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento di Scienze Farmacologiche e Biomolecolari and Center of Excellence on Neurodegenerative Diseases (CEND), Università degli Studi di Milano, Milan 20133, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Treccani
authors.affiliation: Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging,Aarhus University Hospital, Aarhus C 8000, Denmark.
authors.forename: Abdelrahman
authors.initials: A
authors.lastname: Alabsi
authors.affiliation: Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov 8240, Denmark.
authors.forename: Heidi
authors.initials: H
authors.lastname: Kaastrup Mueller
authors.affiliation: Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov 8240, Denmark.
authors.forename: Betina
authors.initials: B
authors.lastname: Elfving
authors.affiliation: Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento di Scienze Farmacologiche e Biomolecolari and Center of Excellence on Neurodegenerative Diseases (CEND), Università degli Studi di Milano, Milan 20133, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Popoli
authors.affiliation: Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov 8240, Denmark.|Pharmaceutical Research Center of Excellence, School of Pharmacy, North-West University, Potchefstroom, South Africa.
authors.forename: Gregers
authors.initials: G
authors.lastname: Wegener
authors.affiliation: Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging,Aarhus University Hospital, Aarhus C 8000, Denmark.
authors.forename: Jens Randel
authors.initials: JR
authors.lastname: Nyengaard
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology*; D001284:Atrophy; D001835:Body Weight; D003345:Corticosterone / Q000097:blood; D003712:Dendrites / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D003891:Desipramine / Q000494:pharmacology*; D004597:Electroshock; D005528:Foot; D008297:Male; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D017966:Pyramidal Cells / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D051339:Actin Depolymerizing Factors; D000929:Antidepressive Agents, Tricyclic; D012333:RNA, Messenger; D003891:Desipramine; D003345:Corticosterone
keywords: Golgi staining; antidepressant; cofilin; dendrite; foot-shock