title: Synchronization of the Budding Yeast Saccharomyces cerevisiae.
abstract: A number of model organisms have provided the basis for our understanding of the eukaryotic cell cycle. These model organisms are generally much easier to manipulate than mammalian cells and as such provide amenable tools for extensive genetic and biochemical analysis. One of the most common model organisms used to study the cell cycle is the budding yeast Saccharomyces cerevisiae. This model provides the ability to synchronise cells efficiently at different stages of the cell cycle, which in turn opens up the possibility for extensive and detailed study of mechanisms regulating the eukaryotic cell cycle. Here, we describe methods in which budding yeast cells are arrested at a particular phase of the cell cycle and then released from the block, permitting the study of molecular mechanisms that drive the progression through the cell cycle.
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas/Universidad de Cantabria, Albert Einstein 22, 39011, Santander, Spain.|Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Foltman
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas/Universidad de Cantabria, Albert Einstein 22, 39011, Santander, Spain.|Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain.
authors.forename: Iago
authors.initials: I
authors.lastname: Molist
authors.affiliation: Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, 39011, Santander, Spain. a.sanchezdiaz@unican.es.|Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas, Universidad de Cantabria, Albert Einstein 22, 39011, Santander, Spain. a.sanchezdiaz@unican.es.
authors.forename: Alberto
authors.initials: A
authors.lastname: Sanchez-Diaz
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000378:metabolism; D005434:Flow Cytometry; D019204:GTP-Binding Proteins / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology; D000072235:Mating Factor; D015739:Nocodazole / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*
chemical_list: C082279:CDC15 protein; D018797:Cell Cycle Proteins; D010455:Peptides; D000072235:Mating Factor; D019204:GTP-Binding Proteins; D015739:Nocodazole; D006918:Hydroxyurea
keywords: Alpha factor; Budding yeast; Early S phase; G1 phase; G2 and M phases; Hydroxyurea; Mitosis; Nocodazole; Saccharomyces cerevisiae; Synchronization; cdc15-2