title: Heterogeneity of miR-10b expression in circulating tumor cells.
abstract: Circulating tumor cells (CTCs) in the blood of cancer patients are recognized as important potential targets for future anticancer therapies. As mediators of metastatic spread, CTCs are also promising to be used as 'liquid biopsy' to aid clinical decision-making. Recent work has revealed potentially important genotypic and phenotypic heterogeneity within CTC populations, even within the same patient. MicroRNAs (miRNAs) are key regulators of gene expression and have emerged as potentially important diagnostic markers and targets for anti-cancer therapy. Here, we describe a robust in situ hybridization (ISH) protocol, incorporating the CellSearch(®) CTC detection system, enabling clinical investigation of important miRNAs, such as miR-10b on a cell by cell basis. We also use this method to demonstrate heterogeneity of such as miR-10b on a cell-by-cell basis. We also use this method to demonstrate heterogeneity of miR-10b in individual CTCs from breast, prostate and colorectal cancer patients.
authors.affiliation: School of Medicine, Deakin University, Geelong, VIC, Australia.
authors.forename: Christin
authors.initials: C
authors.lastname: Gasch
authors.affiliation: School of Medicine, Deakin University, Geelong, VIC, Australia.
authors.forename: Prue N
authors.initials: PN
authors.lastname: Plummer
authors.affiliation: Australian Prostate Cancer Research Centre-Queensland, Queensland University of Technology, Brisbane, QLD, Australia.|Institute of Health and Biomedical Innovation &School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD, Australia.
authors.forename: Lidija
authors.initials: L
authors.lastname: Jovanovic
authors.affiliation: School of Surgery, The University of Western Australia, Perth, WA, Australia.
authors.forename: Linda M
authors.initials: LM
authors.lastname: McInnes
authors.affiliation: School of Medicine, Deakin University, Geelong, VIC, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Wescott
authors.affiliation: School of Surgery, The University of Western Australia, Perth, WA, Australia.
authors.forename: Christobel M
authors.initials: CM
authors.lastname: Saunders
authors.affiliation: National Center for Tumor Diseases, Heidelberg, Germany.|Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Schneeweiss
authors.affiliation: National Center for Tumor Diseases, Heidelberg, Germany.|Department of Obstetrics and Gynecology, University of Heidelberg, Heidelberg Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Wallwiener
authors.affiliation: Australian Prostate Cancer Research Centre-Queensland, Queensland University of Technology, Brisbane, QLD, Australia.|Institute of Health and Biomedical Innovation &School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD, Australia.
authors.forename: Colleen
authors.initials: C
authors.lastname: Nelson
authors.affiliation: Ingham Institute for Applied Medical Research, Liverpool Hospital, Liverpool, NSW, Australia.|Liverpool Clinical School, School of Medicine, Western Sydney University, NSW, Australia.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Spring
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Riethdorf
authors.affiliation: Institute of Health and Biomedical Innovation &School of Biomedical Sciences, Queensland University of Technology, Brisbane, QLD, Australia.|St. Vincent's Institute of Medical Research and Department of Surgery, University of Melbourne, Melbourne, Australia.
authors.forename: Erik W
authors.initials: EW
authors.lastname: Thompson
authors.affiliation: Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Klaus
authors.initials: K
authors.lastname: Pantel
authors.affiliation: School of Medicine, Deakin University, Geelong, VIC, Australia.|School of Medicine, University of New South Wales, NSW, Australia.
authors.forename: Albert S
authors.initials: AS
authors.lastname: Mellick
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D017403:In Situ Hybridization; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C547183:MIRN10 microRNA, human; D035683:MicroRNAs