title: Genetic variants in miR-196a2 and miR-499 are associated with susceptibility to esophageal squamous cell carcinoma in Chinese Han population.
abstract: Esophageal squamous cell carcinoma (ESCC) is the dominant type of esophageal cancer in the East Asian population. MicroRNAs (miRNAs) have been studied to play important roles in tumorigenesis. Single nucleotide polymorphisms (SNPs) in miRNA lead to the aberrant expression and structural alteration of miRNA and are hypothesized to be involved in tumorigenesis and cancer development. We conducted a population-based case-control study to evaluate the association between SNPs in miRNAs and ESCC risk in 1400 ESCC cases and 2185 matched controls. Four SNPs including miR-196a2 rs11614913, miR-146a rs2910164, miR-499 rs3746444, and miR-423 rs6505162 were selected with comprehensive collection strategy and genotyped using the SNaPshot Multiplex System. Odds ratio (OR) and 95 % confidence interval (95 % CI) were used to assess the strength of association. The CC genotype of miR-196a2 rs11614913 was significantly associated with an increased ESCC risk compared with the TT genotype (OR 1.11, 95 % CI 1.01-1.22, P 0.049) and the TT/TC genotypes (OR 1.09, 95 % CI 1.01-1.19, P 0.043). The association was more pronounced in non-drinkers in the recessive model (OR 1.13, 95 % CI 1.01-1.27, P 0.029). A significantly increased risk of ESCC associated with miR-499 rs3746444 polymorphism was evident among patients who never smoking and drinking. This study suggests that miR-196a2 rs11614913 and miR-499 rs3746444 are associated with an increased ESCC risk in a Chinese population.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Shen
authors.affiliation: China National Center for Biotechnology Development, Beijing, China.
authors.forename: Jiejun
authors.initials: J
authors.lastname: Chen
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, 200433, Shanghai, China.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Yabiao
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, 200433, Shanghai, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, 200433, Shanghai, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Chenji
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Dunmei
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Mengyun
authors.initials: M
authors.lastname: Wang
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.|Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.|Department of Thoracic Surgery, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Xiang
authors.affiliation: College of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.|Duke Cancer Institute, Duke University Medical Center, Durham, NC, USA.
authors.forename: Qingyi
authors.initials: Q
authors.lastname: Wei
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, 200433, Shanghai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: MOE Key Laboratory of Contemporary Anthropology and State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Handan Road 220, 200433, Shanghai, China. jcwang@fudan.edu.cn.
authors.forename: Jiucun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Ren'ai Road 199, 215123, Suzhou, China. mhwang@suda.edu.cn.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D008957:Models, Genetic; D020641:Polymorphism, Single Nucleotide; D012372:ROC Curve; D012307:Risk Factors
chemical_list: C542543:MIRN196 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs
keywords: ESCC susceptibility; miR-196a2; miR-499; rs11614913; rs3746444