title: [Expression of HMGB1 and NF-κB in Patients with Acute Leukemia and Its Significance].
abstract: OBJECTIVE
  To explore the expression of high mobility group box protein 1 (HMGB1) and nuclear factor-kappa B (NF-κB) in patients with acute leukemia and its significance.
  
  
  METHODS
  20 samples of bone marrow and peripheral blood from each acute leukemia groups (newly diagnozed, relapsed and complete remission groups) and 20 samples as control from patients with no-hematologic malignancies were collected. The expression level of HMGB1 in peripheral blood plasma was determined by ELISA; HMGB1 and NF-κB level in mononuclear cells were examined by RT-PCR. Western blot was used to determine HMGB1 and NF-κB protein levels. HMGB1 and NF-κB in bone marrow smears were determined by immnohistochemistry method (IHC).
  
  
  RESULTS
  The expression level of HMGB1 obviously increased in patients of newly diagnosed and relapsed groups, as compared with control group there was statistical significance (P < 0.05), but there was no obvious difference in expression level of HMGB1 between complete remission group and control group (P > 0.05). The expression level of HMGB1 and NF-kB in monnuclear cells of bone marrow in newly-diagnosed group and relapsed group was significantly higher than that in control group (P < 0.05), but the expression levels of HMGB1 and NF-kB in complete remisson group did not change (P > 0.05). The results of immnohistochemistry method indicated that the possitive expression of HMGB1 and NF-kB maily was found in bone marrow smears of newly diagnosed and relapsed groups.
  
  
  CONCLUSIONS
  HMGB1 is overexpressed in acute leukemia, which may be involved in the occurrence and development of acute leukemia by activating the NF-κB signaling pathway, HMGB1 may be a important index for observing therapeutic effectiveness and predicting recurrence of acute leukemia.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.|Department of Hematology, Affiliated Hospital of Jinggangshan University, JiAn 343000, Jiangxi Province, China.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Zheng
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.
authors.forename: Liu-Yan
authors.initials: LY
authors.lastname: Xin
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.|Department of Hematology, Affiliated Hospital of Jinggangshan University, JiAn 343000, Jiangxi Province, China.
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Zhong
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China. E-mail: chenyj2005@163.com.
authors.forename: Yi-Jian
authors.initials: YJ
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D015153:Blotting, Western; D001853:Bone Marrow; D016022:Case-Control Studies; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D012074:Remission Induction; D015398:Signal Transduction
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human