title: Mincle, an Innate Immune Receptor, Is Expressed in Urothelial Cancer Cells of Papillomavirus-Associated Urothelial Tumors of Cattle.
abstract: BACKGROUND
  Mincle, macrophage-inducible C-type lectin, is a member of C-type lectin receptors. It plays an important role in anti-mycobacterial and anti-fungal immunity. Furthermore it senses dead cells through its primary ligand SAP130.
  We examined ten urothelial tumors of the urinary bladder of cattle. Eight of them expressed E5 cDNA of bovine papillomaviruses type 2 (BPV-2) and type 13 (BPV-13) that belong to Deltapapillomavirus genus. Two of them were not examined for detection of E5 cDNA. Mincle expression appeared to occur in urothelial neoplastic cells only. No mincle expression was detected in urothelial cells from healthy cattle. Mincle expression was characterized by a membranous pattern in papillary urothelial cancers; isolated and/or clustered urothelial cells showing a strong cytoplasmic immunoreactivity were primarily seen in invasive urothelial cancers.
  
  
  CONCLUSIONS
  This is the first study about the expression of mincle in veterinary oncology and the first report which describes the expression of functional mincle receptor in neoplastic cells in medical literature. As it has been shown that urothelial cancer cells have the ability to function as antigen-presenting cells (APCs), it is conceivable that mincle expression is involved in the presentation of cancer cell antigens to cells of the immune system. Furthermore, since expression of mincle contributes to the control of Mycobacterium bovis BCG infection, this study has exciting clinical implications in comparative medicine keeping in mind that Bacillus Calmette-Guérin (BCG) immunotherapy is currently the most effective treatment of non-muscle invasive bladder cancer in man. Mincle expression in urothelial tumor cells warrants further study to better understand the role, if any, of this receptor in bladder cancer. Future studies will provide insights in the role of mincle receptor of urothelial cancer cells in antitumor immunotherapy.
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Malattie Infettive, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Patologia Generale, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Valeria
authors.initials: V
authors.lastname: Russo
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Patologia Generale, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Iolanda
authors.initials: I
authors.lastname: Esposito
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Patologia Generale, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Dora Maria
authors.initials: DM
authors.lastname: Ceccarelli
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Patologia Generale, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Orlando
authors.initials: O
authors.lastname: Paciello
authors.affiliation: Dipartimento di Medicina Veterinaria e Produzioni Animali, Settore Fisiologia, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Luigi
authors.initials: L
authors.lastname: Avallone
authors.affiliation: Dipartimento di Agraria, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Capparelli
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Napoli, Italia.
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
mesh_terms: D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002277:Carcinoma / Q000378:metabolism* / Q000662:veterinary / Q000821:virology; D002417:Cattle; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000662:veterinary / Q000821:virology; D019459:Urothelium / Q000378:metabolism / Q000473:pathology / Q000821:virology
chemical_list: D011971:Receptors, Immunologic