title: Laccase-modified gold nanorods for electrocatalytic reduction of oxygen.
abstract: cathodes. Nanostructuring was provided by gold nanorods (AuNRs), which were characterized and covalently attached to electrodes made of low-density graphite. The nanostructured electrode was the scaffold for covalent and oriented attachment of ThLc. The bioelectrocatalytic currents measured for oxygen reduction were as high as 0.5 mA/cm(2 and 0.7 mA/cm(2), which were recorded under direct and mediated electron transfer regimes, respectively. )The experimental data were fitted to mathematical models showing that when the O2 is bioelectroreduced at high rotation speed of the electrode the heterogeneous electron transfer step is the rate-liming stage. The electrochemical measurement hints a wider population of non-optimally wired laccases than previously reported for 5–8 nm size Au nanoparticle-modified electrode, which could be due to a larger size of the AuNRs when compared to the laccases as well as their different crystal facets.
authors.affiliation: Instituto de Catálisis y Petroleoquimica, CSIC, c/Marie Curie 2, L10, 28049 Madrid, Spain.
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Bari
authors.affiliation: Biomedical Sciences Faculty of Health and Society, Malmo University, SE-205 06 Malmo, Sweden.
authors.forename: Sergey
authors.initials: S
authors.lastname: Shleev
authors.affiliation: Instituto de Catálisis y Petroleoquimica, CSIC, c/Marie Curie 2, L10, 28049 Madrid, Spain. Electronic address: alopez@icp.csic.es.
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
authors.affiliation: Instituto de Catálisis y Petroleoquimica, CSIC, c/Marie Curie 2, L10, 28049 Madrid, Spain. Electronic address: marcospita@icp.csic.es.
authors.forename: Marcos
authors.initials: M
authors.lastname: Pita
mesh_terms: D055162:Biocatalysis; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite; D042845:Laccase / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008962:Models, Theoretical; D043942:Nanotubes / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry*; D013499:Surface Properties; D055454:Trametes / Q000201:enzymology; D014867:Water / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D014867:Water; D006046:Gold; D006108:Graphite; D042845:Laccase; D010100:Oxygen
keywords: Biocathode; Biofuel cell; Gold nanorod; Graphite; Laccase