title: Using zebrafish in systems toxicology for developmental toxicity testing.
abstract: With the high cost and the long-term assessment of developmental toxicity testing in mammals, the vertebrate zebrafish has become a useful alternative model organism for high-throughput developmental toxicity testing. Zebrafish is also very favorable for the 3R perspective in toxicology; however, the methodologies used by research groups vary greatly, posing considerable challenges to integrative analysis. In this review, we discuss zebrafish developmental toxicity testing, focusing on the methods of chemical exposure, the assessment of morphological abnormalities, housing conditions and their effects on the production of healthy embryos, and future directions. Zebrafish as a systems toxicology model has the potential to elucidate developmental toxicity pathways, and to provide a sound basis for human health risk assessments.
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.|Mie University Medical Zebrafish Research Center, Tsu, Mie.|Department of Systems Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie.|Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Tsu, Mie.|Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie.
authors.forename: Yuhei
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Ono Pharmaceutical Co, Ltd, Osaka, Japan.
authors.forename: Atsuto
authors.initials: A
authors.lastname: Inoue
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.
authors.forename: Shota
authors.initials: S
authors.lastname: Sasagawa
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.
authors.forename: Junko
authors.initials: J
authors.lastname: Koiwa
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.
authors.forename: Koki
authors.initials: K
authors.lastname: Kawaguchi
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.
authors.forename: Reiko
authors.initials: R
authors.lastname: Kawase
authors.affiliation: Ono Pharmaceutical Co, Ltd, Osaka, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Ono Pharmaceutical Co, Ltd, Osaka, Japan.
authors.forename: Soonih
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Molecular and Cellular Pharmacology, Pharmacogenomics and Pharmacoinformatics, Mie University Graduate School of Medicine, Tsu, Mie.|Mie University Medical Zebrafish Research Center, Tsu, Mie.|Department of Systems Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie.|Department of Omics Medicine, Mie University Industrial Technology Innovation Institute, Tsu, Mie.|Department of Bioinformatics, Mie University Life Science Research Center, Tsu, Mie.
authors.forename: Toshio
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D006801:Humans; D013723:Teratogens / Q000633:toxicity*; D018675:Toxicity Tests; D015027:Zebrafish
chemical_list: D013723:Teratogens
keywords: chemical exposure; developmental toxicity; housing; systems toxicology; zebrafish