title: Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.
abstract: During development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is required for dendrite pruning in Drosophila sensory neurons. Spn-F acts downstream of IKK-related kinase Ik2 in the same pathway for dendrite pruning. Spn-F exhibits a punctate pattern in larval neurons, whereas these Spn-F puncta become redistributed in pupal neurons, a step that is essential for dendrite pruning. The redistribution of Spn-F from puncta in pupal neurons requires the phosphorylation of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex. Spn-F is a key component to link Ik2 kinase to dynein motor complex, and the formation of Ik2/Spn-F/dynein complex is critical for Spn-F redistribution and for dendrite pruning. Our findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons.
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Tzu
authors.initials: T
authors.lastname: Lin
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Po-Yuan
authors.initials: PY
authors.lastname: Pan
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Lai
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Kai-Wen
authors.initials: KW
authors.lastname: Chiang
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsin-Lun
authors.initials: HL
authors.lastname: Hsieh
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Wu
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Ke
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Myong-Chol
authors.initials: MC
authors.lastname: Lee
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Shih-Sian
authors.initials: SS
authors.lastname: Liao
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsueh-Tzu
authors.initials: HT
authors.lastname: Shih
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Chiou-Yang
authors.initials: CY
authors.lastname: Tang
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, United States of America.
authors.forename: Shi-Bing
authors.initials: SB
authors.lastname: Yang
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Hsu-Chen
authors.initials: HC
authors.lastname: Cheng
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: June-Tai
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Howard Hughes Medical Institute, Department of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, United States of America.
authors.forename: Yuh-Nung
authors.initials: YN
authors.lastname: Jan
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: Hsiu-Hsiang
authors.initials: HH
authors.lastname: Lee
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D003712:Dendrites / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism / Q000502:physiology*; D004331:Drosophila melanogaster; D004398:Dyneins / Q000378:metabolism; D051550:I-kappa B Kinase / Q000378:metabolism*; D010766:Phosphorylation; D011984:Sensory Receptor Cells / Q000166:cytology*
chemical_list: D029721:Drosophila Proteins; D051550:I-kappa B Kinase; C510861:IKKepsilon protein, Drosophila; D004398:Dyneins