title: Cutaneous myxidiosis in European eel, Anguilla anguilla (Linnaeus, 1758): histopathology, histochemistry and laminin immunohistochemistry.
abstract: Histopathology, histochemistry and immunohistochemistry of the integument of European eel, Anguilla anguilla (Linnaeus, 1758), infected by Myxidium sp. are reported. Skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with H&E, Periodic acid-Schiff's staining method, Alcian Blue 8 GX pH 2.5/Periodic acid-Schiff's and McCallum-Goodpasture's Gram stain. Moreover, immunohistochemistry was performed using a primary polyclonal laminin antibody. Histologically, cysts (diameter 2-3 mm) were observed mainly under the scale pockets, encircled by a thin collagen layer, lined by elongated, flattened fibroblasts and containing bipolar, PAS- and Gram-positive spores with opposite polar capsules. The epidermis stretched by the underlying cyst appeared dysplastic, thinned with a significant reduction in mucous cells number. Only inconsistent and aspecific inflammatory reaction was noted around the cysts at the dermis/epidermis interface. Intense laminin-like protein immunolabel was documented in the plasmodial ectoplasm and related to host anergia. This was the first report of laminin immunolabel in a member of the Myxozoa. Epidermal dysplasia represents likely an aspecific response against the underlying tensile force exerted by the developing parasite cyst, while fibroblast and collagen encapsulation denote a parasite-driven host response protecting, rather than harming, the encircled parasite.
authors.affiliation: Faculty of Biosciences, Food and Environmental Technologies, University of Teramo, Teramo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Manera
authors.affiliation: Small Animal Praxis, Roseto degli Abruzzi, TE, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Borreca
authors.affiliation: Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
authors.forename: B S
authors.initials: BS
authors.lastname: Dezfuli
mesh_terms: D000811:Anguilla; D000818:Animals; D005393:Fish Diseases / Q000469:parasitology / Q000473:pathology*; D007150:Immunohistochemistry / Q000662:veterinary; D007797:Laminin / Q000378:metabolism*; D055764:Myxozoa / Q000502:physiology*; D010273:Parasitic Diseases, Animal / Q000469:parasitology / Q000473:pathology*; D012871:Skin Diseases / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
chemical_list: D007797:Laminin
keywords: Myxidium spp.; histochemistry; histopathology; immune evasion; immunohistochemistry; laminin