title: Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia.
abstract: Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx 2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx 1a, stx 2a, stx 2c, and ehxA and the other carrying stx 1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
authors.forename: Asanthi
authors.initials: A
authors.lastname: Perera
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
authors.forename: Charles M
authors.initials: CM
authors.lastname: Clarke
authors.affiliation: School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Dykes
authors.affiliation: CSIRO, Food and Nutrition Flagship, 671 Sneydes Road, Werribee, VIC 3030, Australia.
authors.forename: Narelle
authors.initials: N
authors.lastname: Fegan
mesh_terms: D000818:Animals; D004927:Escherichia coli Infections / Q000235:genetics* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008296:Malaysia; D012418:Ruminants / Q000382:microbiology; D012703:Serotyping; D022621:Shiga Toxin / Q000235:genetics; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022621:Shiga Toxin