title: Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins.
abstract: Multiple membrane-binding proteins are key players in cytokinesis in yeast and other organisms. In vivo techniques for analyzing protein-membrane interactions are currently limited. In vitro assays allow characterization of the biochemical properties of these proteins to build a mechanistic understanding of protein-membrane interactions during cytokinesis. Here, we describe two in vitro assays to characterize FCH-Bin/Amphyphysin/RVS (F-BAR) domains and other protein's interactions with membranes: liposome co-pelleting and giant unilamellar vesicle fluorescent binding.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 465 21st Ave S., Nashville, TN, 37240-7935, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: McDonald
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, 465 21st Ave S., Nashville, TN, 37240-7935, USA. kathy.gould@vanderbilt.edu.
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Gould
mesh_terms: D048749:Cytokinesis; D008081:Liposomes; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs
chemical_list: D008081:Liposomes; D008565:Membrane Proteins
keywords: F-BAR domain; Giant unilamellar vesicle s; Liposomes; Membrane bind ing