title: Marinobacter aromaticivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment.
abstract: A rod-shaped, Gram-stain-negative, slightly halotolerant bacterium, designated strain D15-8PT, was isolated from a sediment sample from the South China Sea. The strain could grow in NaCl concentrations ranging from 0.5 % to 10 % (w/v) (optimum 0.5-1.5 %), and could be cultivated at 10-40 °C (optimum 25 °C) and pH 5.5-9.5 (optimum pH 7.0-8.0). The strain was positive for catalase, oxidase, and hydrolysis of Tween 80, but negative for hydrolysis of DNA and gelatin, nitrite reduction, indole production, Voges-Proskauer reaction, and methyl red test. Strain D15-8PT could biodegrade naphthalene, phenanthrene, and anthracene. The major respiratory quinone was Q-9. The main cellular fatty acids were C12 : 0 (11.5 %), C14 : 0 3-methyl (22.0 %), C16 : 0 (19.2 %), C16 : 1ω9c (22.9 %), and C18 : 1ω9c (6.7 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G+C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA genes showed that strain D15-8PT was most closely related to Marinobacter maritimus JCM 12521T (98.5 % 16S rRNA gene sequence similarity), Marinobacter antarcticus CGMCC 1.10835T (98.1 %), Marinobacter lipolyticus DSM 15157T (97.1 %), and Marinobacter guineae CECT 7243T (97.0 %). Results of the gyrB gene analysis and DNA-DNA hybridization were both less than the cut-off values (90 % for gyrB gene sequence similarity and 70 % for DNA-DNA hybridization). On the basis of this taxonomic study using a polyphasic approach, strain D15-8PT represents a novel species of the genus Marinobacter, for which the name Marinobacter aromaticivorans sp. nov. is proposed. The type strain is D15-8PT ( = CGMCC 1.11015T = KCTC 23781T).
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.|College of Marine life, Ocean University of China, Qingdao, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Guangfei
authors.initials: G
authors.lastname: Xu
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luan
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Yin
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.
authors.forename: Deming
authors.initials: D
authors.lastname: Huang
authors.affiliation: Marine Ecology Research Center, First Institute of Oceanography, State Oceanic Administration of China, Qingdao, PR China.|Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
mesh_terms: D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9