title: Multi-Omic Dynamics Associate Oxygenic Photosynthesis with Nitrogenase-Mediated H2 Production in Cyanothece sp. ATCC 51142.
abstract: To date, the proposed mechanisms of nitrogenase-driven photosynthetic H2 production by the diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142 have assumed that reductant and ATP requirements are derived solely from glycogen oxidation and cyclic-electron flow around photosystem I. Through genome-scale transcript and protein profiling, this study presents and tests a new hypothesis on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in Cyanothece 51142. Our results show that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized with nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the role of concurrent photocatalytic H2O oxidation as a participating process.
authors.affiliation: Chemical and Biological Signature Science, Pacific Northwest National Laboratory, Richland, WA 99352.|Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Hans C
authors.initials: HC
authors.lastname: Bernstein
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Moiz A
authors.initials: MA
authors.lastname: Charania
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Ryan S
authors.initials: RS
authors.lastname: McClure
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Sadler
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Melnicki
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Hill
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Lye Meng
authors.initials: LM
authors.lastname: Markillie
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Wright
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Margaret F
authors.initials: MF
authors.lastname: Romine
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA, 99352.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Beliaev
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D046889:Cyanothece / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D020869:Gene Expression Profiling; D006003:Glycogen / Q000737:chemistry / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex; D012333:RNA, Messenger; D014867:Water; D006859:Hydrogen; D006003:Glycogen; D006864:Hydrogenase; D009591:Nitrogenase; D010100:Oxygen