title: Luminescent silica nanoparticles for sensing acetylcholinesterase-catalyzed hydrolysis of acetylcholine.
abstract: This work highlights the H-function of Tb(III)-doped silica nanoparticles in aqueous solutions of acetic acid as a route to sense acetylcholinesterase-catalyzed hydrolysis of acetylcholine (ACh). The H-function results from H(+)-induced quenching of Tb(III)-centered luminescence due to protonation of Tb(III) complexes located close to silica/water interface. The H-function can be turned on/switched off by the concentration of complexes within core or nanoparticle shell zones, by the silica surface decoration and adsorption of both organic and inorganic cations on silica surface. Results indicate the optimal synthetic procedure for making nanoparticles capable of sensing acetic acid produced by enzymatic hydrolysis of acetylcholine. The H-function of nanoparticles was determined at various concentrations of ACh and AChE. The measurements show experimental conditions for fitting the H-function to Michaelis-Menten kinetics. Results confirm that reliable fluorescent monitoring AChE-catalyzed hydrolysis of ACh is possible through the H-function properties of Tb(III)-doped silica nanoparticles.
authors.affiliation: Kazan Federal University, Kremlyovskaya Street 18, 420008 Kazan, Russian Federation.
authors.forename: Alsu R
authors.initials: AR
authors.lastname: Mukhametshina
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Fedorenko
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Zueva
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Petrov
authors.affiliation: Kazan Federal University, Kremlyovskaya Street 18, 420008 Kazan, Russian Federation.
authors.forename: Patrick
authors.initials: P
authors.lastname: Masson
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
authors.forename: Irek R
authors.initials: IR
authors.lastname: Nizameev
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation. Electronic address: asiyamust@mail.ru.
authors.forename: Asiya R
authors.initials: AR
authors.lastname: Mustafina
authors.affiliation: A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Street 8, 420088 Kazan, Russian Federation.
authors.forename: Oleg G
authors.initials: OG
authors.lastname: Sinyashin
mesh_terms: D000109:Acetylcholine / Q000737:chemistry*; D000110:Acetylcholinesterase / Q000032:analysis* / Q000737:chemistry; D002384:Catalysis; D004867:Equipment Design; D019544:Equipment Failure Analysis; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide; D000110:Acetylcholinesterase; D000109:Acetylcholine
keywords: Acetylcholine; Acetylcholinesterase; Enzymatic hydrolysis; Luminescence; Silica nanoparticles; Tb(III) complex