title: Photoprotective Potential of Penta-O-Galloyl-β-DGlucose by Targeting NF-κB and MAPK Signaling in UVB Radiation-Induced Human Dermal Fibroblasts and Mouse Skin.
abstract: Exposure of the skin to ultraviolet radiation can cause skin damage with various pathological changes including inflammation. In the present study, we identified the skin-protective activity of 1,2,3,4,6-penta-O-galloyl-β-D-glucose (pentagalloyl glucose, PGG) in ultraviolet B (UVB) radiation-induced human dermal fibroblasts and mouse skin. PGG exhibited antioxidant activity with regard to intracellular reactive oxygen species (ROS) generation as well as ROS and reactive nitrogen species (RNS) scavenging. Furthermore, PGG exhibited anti-inflammatory activity, inhibiting the activation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, resulting in inhibition of the expression of pro-inflammatory mediators. Topical application of PGG followed by chronic exposure to UVB radiation in the dorsal skin of hairless mice resulted in a significant decrease in the progression of inflammatory skin damages, leading to inhibited activation of NF-κB signaling and expression of pro-inflammatory mediators. The present study demonstrated that PGG protected from skin damage induced by UVB radiation, and thus, may be a potential candidate for the prevention of environmental stimuli-induced inflammatory skin damage.
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.|Biomedical Science Project (BK21 PLUS), Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Byung-Hak
authors.initials: BH
authors.lastname: Kim
authors.affiliation: Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan 38610, Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Choi
authors.affiliation: Department of Microbiology and Immunology, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Song-Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Kum Hee
authors.initials: KH
authors.lastname: Noh
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Sunho
authors.initials: S
authors.lastname: Kwon
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Ae Jin
authors.initials: AJ
authors.lastname: Jeong
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Haeri
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.|Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Yi
authors.affiliation: Industry-Academic Cooperation Foundation, Hanbat National University, Daejeon 305-719, Korea.
authors.forename: Jung Youl
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Cosmeceutical Science, Daegu Haany University, Gyeongsan 38610, Korea.
authors.forename: Jintae
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Herbal Biotechnology, Daegu Haany University, Gyeongsan 38610, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Joo
authors.affiliation: Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.|Biomedical Science Project (BK21 PLUS), Seoul National University College of Medicine, Seoul 110-799, Korea.|Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.|Neuro-Immune Information Storage Network Research Center, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Sang-Kyu
authors.initials: SK
authors.lastname: Ye
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D011855:Radiodermatitis / Q000517:prevention & control*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D047348:Hydrolyzable Tannins; D016328:NF-kappa B; D013473:Sunscreening Agents; C435084:pentagalloylglucose; D020928:Mitogen-Activated Protein Kinases
keywords: 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG); inflammation; mitogen-activated protein kinase (MAPK); nuclear factor-kappaB (NF-κB); reactive oxygen species (ROS); ultraviolet B (UVB)