title: Ex Vivo and In Situ Evaluation of 'Dispelling-Wind' Chinese Medicine Herb-Drugs on Intestinal Absorption of Chlorogenic Acid.
abstract: This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used 'dispelling-wind' TCM botanical drugs [roots of Angelica dahurica (Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) and Saposhnikovia divaricata (Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance. Ex vivo everted intestinal sac and in situ single pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P-gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the P(app) and K(a) of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P-gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions.
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.|Centre for Pharmacognosy and Phytotherapy, Research Cluster Biodiversity and Medicines, UCL School of Pharmacy, University of London, UK.
authors.forename: Lixiang
authors.identifier: http://orcid.org/0000-0001-5281-790X
authors.initials: L
authors.lastname: Zhai
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Weitong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Centre for Pharmacognosy and Phytotherapy, Research Cluster Biodiversity and Medicines, UCL School of Pharmacy, University of London, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Heinrich
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
authors.forename: Wenji
authors.initials: W
authors.lastname: Deng
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000037:antagonists & inhibitors; D029969:Angelica / Q000737:chemistry; D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D002726:Chlorogenic Acid / Q000378:metabolism*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014700:Verapamil / Q000494:pharmacology
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D002726:Chlorogenic Acid; D014700:Verapamil
keywords: Angelica dahurica; Saposhnikovia divaricata; chlorogenic acid; everted rat gut sac; intestinal absorption; single-pass intestinal perfusion