title: Electroanalysis of Shewanella oneidensis MR-1.
abstract: Electrochemical parameters of bacterial cells Shewanella oneidensis MR-1 were investigated. For registration of the direct electron transfer between S. oneidensis MR-1 and electrode, bacterial cells were pretreated with didodecyldimethylammonium bromide (DDAB), a synthetic membrane-like substance of polycationic nature that exhibits membrane-loosening properties. Such pretreatment of S. oneidensis MR-1 allowed increasing the efficiency of extracellular electron transfer by the proteobacterium due to better availability of electroactive proteins for registration of electron transfer processes. The electroanalysis of bacterial cells S. oneidensis MR-1 under anaerobic conditions allows registering redox-active proteins and biomolecules in the range of potentials of-0.40,-0.16, and-0 V, which corresponds to flavohemoproteins, quinone derivatives, and c-type cytochromes of the external membrane of S. oneidensis MR-1 cells.
authors.affiliation: Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, ul. Pogodinskaya 10/8, Moscow, 119121, Russia. viktoria.shumyantseva@ibmc.msk.ru.
authors.forename: V V
authors.initials: VV
authors.lastname: Shumyantseva
authors.affiliation: Moscow State University, Moscow, 119991, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Shebanova
authors.affiliation: Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, ul. Pogodinskaya 10/8, Moscow, 119121, Russia.
authors.forename: Ya M
authors.initials: YM
authors.lastname: Chalenko
authors.affiliation: State Research Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi pr. 1, Moscow, 117545, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Voeikova
authors.affiliation: Moscow State University, Moscow, 119991, Russia.
authors.forename: M P
authors.initials: MP
authors.lastname: Kirpichnikov
authors.affiliation: Moscow State University, Moscow, 119991, Russia.|Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119991, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Shaitan
authors.affiliation: State Research Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi pr. 1, Moscow, 117545, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Debabov
mesh_terms: D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D005110:Extracellular Space / Q000737:chemistry / Q000187:drug effects; D006108:Graphite / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D020592:Shewanella / Q000737:chemistry* / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D000644:Quaternary Ammonium Compounds; D014867:Water; C046112:didodecyldimethylammonium; D006108:Graphite