title: Multiple thrombophilic single nucleotide polymorphisms lack a significant effect on outcomes in fresh IVF cycles: an analysis of 1717 patients.
abstract: OBJECTIVE
  The aim of the study is to determine if thrombophilic single nucleotide polymorphisms (SNPs) affect outcomes in fresh in vitro fertilization (IVF) cycles in a large general infertility population.
  
  
  METHODS
  A prospective cohort analysis was performed at a university-affiliated private IVF center of female patients undergoing fresh non-donor IVF cycles. The effect of the following thrombophilic SNPs on IVF outcomes were explored: factor V (Leiden and H1299R), prothrombin (G20210A), factor XIII (V34L), β-fibrinogen (-455G → A), plasminogen activator inhibitor-1 (4G/5G), human platelet antigen-1 (a/b9L33P), and methylenetetrahydrofolate reductase (C677T and A1298C). The main outcome measures included positive pregnancy test, clinical pregnancy, embryo implantation, live birth, and pregnancy loss.
  
  
  RESULTS
  Patients (1717) were enrolled in the study, and a total of 4169 embryos were transferred. There were no statistically significant differences in positive pregnancy test, clinical pregnancy, embryo implantation, live birth, or pregnancy loss in the analysis of 1717 patients attempting their first cycle of IVF. Receiver operator characteristics and logistic regression analyses showed that outcomes cannot be predicted by the cumulative number of thrombophilic mutations present in the patient.
  
  
  CONCLUSIONS
  Individual and cumulative thrombophilic SNPs do not affect IVF outcomes. Therefore, initial screening for these SNPs is not indicated.
authors.affiliation: National Institutes of Health, Bethesda, MD, USA.
authors.forename: George
authors.initials: G
authors.lastname: Patounakis
authors.affiliation: Mount Sinai School of Medicine, New York, NY, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Bergh
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA.|Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Forman
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Lonczak
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA.|Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Franasiak
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA.|Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Treff
authors.affiliation: Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA. rscott@rmanj.com.|Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA. rscott@rmanj.com.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
mesh_terms: D000328:Adult; D016824:Antigens, Human Platelet / Q000235:genetics; D010064:Embryo Implantation / Q000235:genetics; D005165:Factor V / Q000235:genetics; D005176:Factor XIII / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D005340:Fibrinogen / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D007246:Infertility / Q000235:genetics* / Q000473:pathology; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D009154:Mutation; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000235:genetics*; D011516:Prothrombin / Q000235:genetics; D019851:Thrombophilia / Q000235:genetics* / Q000473:pathology
chemical_list: D016824:Antigens, Human Platelet; C000592498:FGB protein, human; D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; D005165:Factor V; D011516:Prothrombin; D005340:Fibrinogen; D005176:Factor XIII; D042965:Methylenetetrahydrofolate Reductase (NADPH2)
keywords: IVF outcomes; Infertility; Pregnancy; SNP; Thrombophilia