title: Components of chicken egg white extract smaller than 3 kDa in size promote 293T cell proliferation.
abstract: We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations. The effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). We demonstrate that components from chicken egg white smaller than 3 kDa in size are able to function as active ingredients promoting cellular proliferation. This discovery may identify a new and convenient additive for cell culture media to promote cell growth and proliferation.
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China. ruangp@126.com.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China. ruangp@126.com.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China. ruangp@126.com.
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Ruan
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Yao
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Jin-Xiang
authors.initials: JX
authors.lastname: Wang
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Ju-Fen
authors.initials: JF
authors.lastname: Liu
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Shu
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Zi-An
authors.initials: ZA
authors.lastname: Li
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China.
authors.forename: Rong-Qing
authors.initials: RQ
authors.lastname: Pang
authors.affiliation: The Cell Biological Therapy Center, Kunming General Hospital of Chengdu Military Command, Kunming, 650032, China. ynkmry@163.com.|Stem Cells and Immune Cells Biomedical Techniques Integrated Engineering Laboratory of State and Regions (Yunnan Province), Kunming, 650032, China. ynkmry@163.com.|Cell Therapy Technology Transfer Medical Key Laboratory of Yunnan Province, Kunming, 650032, China. ynkmry@163.com.
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Pan
keywords: Active ingredients; Cell proliferation; Chicken egg white extract; Separation