title: MTHFR C677T and A1298C polymorphisms as predictors of radiotherapy response in head and neck squamous cell carcinoma.
abstract: The C677T and A1298C polymorphisms in methylene-tetrahydrofolate reductase (MTHFR), which regulates the release of active folate in the body, may have reduced activity. Given that folate participates in important intracellular pathways, such as nucleotide synthesis and biomolecule methylation, it seems plausible that patients with head and neck squamous cell carcinoma (HNSCC) may respond differently to radiotherapy treatments, based on genetic polymor-phisms. Therefore, this study sought to understand the role of these polymorphisms in HNSCC patient radiotherapy response. Genotypes were detected by PCR-RFLP after extraction of DNA from peripheral blood lymphocytes. Survival curves were analyzed by the Kaplan- Meier model, and significant differences were analyzed by the Wil-coxon test. Response to radiotherapy in patients with laryngeal SCC was significantly associated with the MTHFR C677T polymorphism (P = 0.030). Indeed, the presence of at least one T allele decreases the mortality rate up to 3-fold. Therefore, we propose that MTHFR C677T may represent a putative biomarker for radiotherapy prognosis in la-ryngeal SCC patients.
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: Q S
authors.initials: QS
authors.lastname: Anders
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: E
authors.initials: E
authors.lastname: Stur
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: L P
authors.initials: LP
authors.lastname: Agostini
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: F M
authors.initials: FM
authors.lastname: Garcia
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: R S
authors.initials: RS
authors.lastname: Reis
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: J A
authors.initials: JA
authors.lastname: Santos
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: S O
authors.initials: SO
authors.lastname: Mendes
authors.affiliation: Departamento de Biologia, Universidade Federal do Espírito Santo, Alegre, ES, Brasil.
authors.forename: L L
authors.initials: LL
authors.lastname: Maia
authors.affiliation: Departamento de Biologia, Universidade Federal do Espírito Santo, Alegre, ES, Brasil.
authors.forename: G T
authors.initials: GT
authors.lastname: Peterle
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: V
authors.initials: V
authors.lastname: Stange
authors.affiliation: Laboratório de Biologia Molecular, Hospital Heliópolis, São Paulo, SP, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Carvalho
authors.affiliation: Departamento de Biologia Molecular, Faculdade de Medicina, São José do Rio Preto, SP, Brasil.
authors.forename: E H
authors.initials: EH
authors.lastname: Tajara
authors.affiliation: Departamento de Medicina, Universidade Federal do Rio Grande do Norte, Campus Caicó, Caicó, RN, Brasil.
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: A M A
authors.initials: AM
authors.lastname: Silva-Conforti
authors.affiliation: Núcleo de Genética Humana e Molecular, Departamento de Biologia, Universidade Federal do Espírito Santo.
authors.forename: I D
authors.initials: ID
authors.lastname: Louro
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000532:radiotherapy*; D005260:Female; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000532:radiotherapy*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D000077195:Squamous Cell Carcinoma of Head and Neck; D016896:Treatment Outcome
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)