title: Visual and Real-Time Event-Specific Loop-Mediated Isothermal Amplification Based Detection Assays for Bt Cotton Events MON531 and MON15985.
abstract: Bt cotton events MON531 and MON15985 are authorized for commercial cultivation in more than 18 countries. In India, four Bt cotton events have been commercialized; more than 95% of total area under genetically modified (GM) cotton cultivation comprises events MON531 and MON15985. The present study reports on the development of efficient event-specific visual and real-time loop-mediated isothermal amplification (LAMP) assays for detection and identification of cotton events MON531 and MON15985. Efficiency of LAMP assays was compared with conventional and real-time PCR assays. Real-time LAMP assay was found time-efficient and most sensitive, detecting up to two target copies within 35 min. The developed real-time LAMP assays, when combined with efficient DNA extraction kit/protocol, may facilitate onsite GM detection to check authenticity of Bt cotton seeds.
authors.forename: Gurinder Jit
authors.initials: GJ
authors.lastname: Randhawa
authors.forename: Rashmi
authors.initials: R
authors.lastname: Chhabra
authors.forename: Rajesh K
authors.initials: RK
authors.lastname: Bhoge
authors.forename: Monika
authors.initials: M
authors.lastname: Singh
mesh_terms: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001681:Biological Assay / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification; D003368:Gossypium / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000302:isolation & purification; D007194:India; D007300:Insect Control; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D009691:Nucleic Acid Denaturation; D030821:Plants, Genetically Modified; D019076:Transgenes
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D017931:DNA Primers; D018744:DNA, Plant; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis