title: Failure of a single dose of medroxyprogesterone acetate to induce uterine infertility in postnatally treated domestic cats.
abstract: In mice and sheep, neonatal administration of progesterone or progestins inhibited development of uterine glands. The aims of the present study were (1) to describe uterine gland development on postnatal Days 6 to 8 and (2) to evaluate the effects of a single postnatal administration of a progestin on reproduction and adult uterine glands morphology and function in domestic cats. Necropsy was performed on three 1-week-old female cats which had died unrelated to this study. Ten female kittens were randomly assigned within the first 24 hours of birth to: medroxyprogesterone acetate 10 mg/animal subcutaneously (MPA; n = 6) or placebo (PLC; n = 4) and followed up until puberty when they were mated. Twenty-four days after the end of estrus, ovulation and pregnancy were diagnosed by serum progesterone measurement and ultrasonography, respectively. Then, all the cats were ovariohysterectomized. After necropsy or surgery, the excised organs were histologically evaluated. Seven queens ovulated (4 of 6 MPA and 3 of 4 PLC; P > 0.1) and were pregnant (P > 0.1). Four MPA cats presented endometrial hyperplasia and one of them developed a pyometra. The 1-week-old females presented uterine glands in the stage of budding and incipient penetration of the glandular epithelium into the underlying stroma. The MPA-treated queens revealed that the area occupied by uterine glands per square-micrometer (0.55 ± 0.2 vs. 0.49 ± 0.2; P > 0.1) and the height of the glandular epithelium (μm; 24.5 ± 6.7 vs. 24.4 ± 7.2; P > 0.1) did not differ from those of the PLC group. Neither significant gross nor microscopical differences were also found for ovaries (P > 0.1). It is concluded that 1-week-old kittens had an incipient stage of uterine gland development and that a single postnatal supraphysiological dose of MPA did not alter uterine adenogenesis in this species. Furthermore, this treatment seemed to predispose to uterine disease without prevention of fertility.
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Lopez Merlo
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Faya
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina.
authors.forename: P G
authors.initials: PG
authors.lastname: Blanco
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Carranza
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Barbeito
authors.affiliation: Laboratory of Reproductive Physiology, Faculty of Veterinary Medicine, National University of La Plata-CONICET, La Plata, Argentina. Electronic address: cgobello@fcv.unlp.edu.ar.
authors.forename: C
authors.initials: C
authors.lastname: Gobello
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002415:Cats; D003271:Contraceptive Agents, Female / Q000008:administration & dosage / Q000494:pharmacology*; D004334:Drug Administration Schedule; D005260:Female; D017258:Medroxyprogesterone Acetate / Q000008:administration & dosage / Q000494:pharmacology*; D011247:Pregnancy; D012741:Sexual Maturation; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects
chemical_list: D003271:Contraceptive Agents, Female; D017258:Medroxyprogesterone Acetate
keywords: Endocrine disruption; Felid; Genital tract