title: Cell-based screens and phenomics with fission yeast.
abstract: Next-generation sequencing approaches have considerably advanced our understanding of genome function and regulation. However, the knowledge of gene function and complex cellular processes remains a challenge and bottleneck in biological research. Phenomics is a rapidly emerging area, which seeks to rigorously characterize all phenotypes associated with genes or gene variants. Such high-throughput phenotyping under different conditions can be a potent approach toward gene function. The fission yeast Schizosaccharomyces pombe (S. pombe) is a proven eukaryotic model organism that is increasingly used for genomewide screens and phenomic assays. In this review, we highlight current large-scale, cell-based approaches used with S. pombe, including computational colony-growth measurements, genetic interaction screens, parallel profiling using barcodes, microscopy-based cell profiling, metabolomic methods and transposon mutagenesis. These diverse methods are starting to offer rich insights into the relationship between genotypes and phenotypes.
authors.affiliation: a Research Department of Genetics , Evolution and Environment and UCL Institute of Healthy Ageing, University College London , London , UK.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Rallis
authors.affiliation: a Research Department of Genetics , Evolution and Environment and UCL Institute of Healthy Ageing, University College London , London , UK.
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D004251:DNA Transposable Elements; D016679:Genome, Viral; D055432:Metabolomics; D008954:Models, Biological; D016296:Mutagenesis; D010641:Phenotype; D012568:Schizosaccharomyces / Q000145:classification / Q000235:genetics*
chemical_list: D000596:Amino Acids; D004251:DNA Transposable Elements
keywords: Drug screen; functional profiling; metabolomics; microbial growth; mutant screen