title: Development and Validation of an HPLC-UV Method for Determination of Eight Phenolic Compounds in Date Palms.
abstract: A simple, precise, accurate, and selective method was developed and validated for determination of eight phenolic compounds (gallic acid, p-hydroxybenzoic acid, vanilic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, and sinapic acid) in date palms. Separation was achieved on an RP C18 column using the mobile phase methanol-water with 2% acetic acid (18+82, v/v). This method was validated according to the requirements for new methods, which include accuracy, precision, selectivity, robustness, LOD, LOQ, linearity, and range. The method demonstrated good linearity over the range 1-1000 ppm of gallic acid, p-hydroxybenzoic acid, vanilic acid, caffeic acid, and syringic acid with r2 greater than 0.99, and in the range of 3-1000 ppm for p-coumaric acid, ferulic acid, and sinapic acid with r2 greater than 0.99. The recovery of the eight phenolic compounds ranged from 97.1 to 102.2%. The method is selective because adjacent peaks of phenolic compounds were well separated with good resolution. The degree of reproducibility of the results obtained as a result of small deliberate variations in the method parameters and by changing analytical operators proved that the method is robust and rugged.
authors.affiliation: Al-Quds University, Faculty of Science and Technology, PO Box 20002, East Jerusalem, Palestinian Authority.
authors.forename: Fuad
authors.initials: F
authors.lastname: Al-Rimawi
authors.forename: Imad
authors.initials: I
authors.lastname: Odeh
mesh_terms: D002109:Caffeic Acids / Q000032:analysis; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D003373:Coumaric Acids / Q000032:analysis; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000032:analysis; D010226:Parabens / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D065749:Phoeniceae / Q000737:chemistry*; D011422:Propionates; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D010226:Parabens; D010636:Phenols; D011422:Propionates; D005707:Gallic Acid; C073734:sinapinic acid; C004999:ferulic acid; C001945:syringic acid; C495469:p-coumaric acid; C038193:4-hydroxybenzoic acid; C040048:caffeic acid