title: Spiroindole Alkaloids and Spiroditerpenoids from Aspergillus duricaulis and Their Potential Neuroprotective Effects.
abstract: Six new spiroindole alkaloids (1-6) and two new spiroditerpenoids (7 and 8) were isolated from an EtOAc extract of Aspergillus duricaulis culture media together with five known compounds. The structures of the isolated compounds were elucidated by analysis of NMR and MS data, and the absolute configurations of compounds 1-8 were confirmed by CD spectroscopic methods. All isolated compounds were evaluated for their inhibition of beta-amyloid (Aβ) aggregate-induced toxicity in PC12 cells and Aβ aggregation. Compounds 8-11 efficiently protected PC12 cells against Aβ aggregate-induced toxicity, but only compound 9 inhibited Aβ aggregation. On the other hand, compounds 4 and 5 exhibited moderate inhibitory effects on Aβ aggregation, but did not protect the cells from Aβ aggregate-induced toxicity. Preincubating Aβ monomers with compounds 4 and 5 rescued PC12 cells against Aβ aggregate-induced toxicity by reducing neurotoxic Aβ aggregates. Compound 9 inhibited both Aβ aggregate-induced toxicity and Aβ aggregation.
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Young Hye
authors.initials: YH
authors.lastname: Seo
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Dankook University , Cheonan 31116, Republic of Korea.
authors.forename: Jae-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Ewha Womans University , Seoul 03760, Republic of Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Seo
authors.affiliation: Tianjin Key Laboratory of Molecular Drug Research, College of Pharmacy, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Molecular Drug Research, College of Pharmacy, Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Korean Agricultural Culture Collection, National Academy of Agricultural Science , Wanju 55365, Republic of Korea.
authors.forename: Seung-Beom
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Dankook University , Cheonan 31116, Republic of Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors; D000818:Animals; D001230:Aspergillus / Q000187:drug effects; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D016716:PC12 Cells; D051381:Rats; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D004224:Diterpenes; D026121:Indole Alkaloids; D018696:Neuroprotective Agents; D013141:Spiro Compounds