title: Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments.
abstract: To accurately measure gene expression using quantitative reverse transcription PCR (qRT-PCR), reliable reference gene(s) are required for data normalization. Corchorus capsularis, an annual herbaceous fiber crop with predominant biodegradability and renewability, has not been investigated for the stability of reference genes with qRT-PCR. In this study, 11 candidate reference genes were selected and their expression levels were assessed using qRT-PCR. To account for the influence of experimental approach and tissue type, 22 different jute samples were selected from abiotic and biotic stress conditions as well as three different tissue types. The stability of the candidate reference genes was evaluated using geNorm, NormFinder, and BestKeeper programs, and the comprehensive rankings of gene stability were generated by aggregate analysis. For the biotic stress and NaCl stress subsets, ACT7 and RAN were suitable as stable reference genes for gene expression normalization. For the PEG stress subset, UBC, and DnaJ were sufficient for accurate normalization. For the tissues subset, four reference genes TUBβ, UBI, EF1α, and RAN were sufficient for accurate normalization. The selected genes were further validated by comparing expression profiles of WRKY15 in various samples, and two stable reference genes were recommended for accurate normalization of qRT-PCR data. Our results provide researchers with appropriate reference genes for qRT-PCR in C. capsularis, and will facilitate gene expression study under these conditions.
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Niu
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Qi
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China ; College of Life Sciences, Shangrao Normal University Shangrao, China.
authors.forename: Gaoyang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Jiantang
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Aifen
authors.initials: A
authors.lastname: Tao
authors.affiliation: Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University Fuzhou, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Fang
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences Changsha, China.
authors.forename: Jianguang
authors.initials: J
authors.lastname: Su
keywords: abiotic and biotic stress; gene expression; jute (Corchorus capsularis); qRT-PCR; reference genes