title: Production of Hyaluronic Acid by Streptococcus zooepidemicus on Protein Substrates Obtained from Scyliorhinus canicula Discards.
abstract: This work investigates the production of hyaluronic acid (H) by Streptococcus equi subsp. zooepidemicus in complex media formulated with peptones obtained from Scyliorhinus canicula viscera by-products. Initially, in batch cultures, the greatest productions were achieved using commercial media (3.03 g/L) followed by peptones from alcalase hydrolyzed viscera (2.32 g/L) and peptones from non-hydrolyzed viscera (2.26 g/L). An increase of between 12% and 15% was found in subsequent fed-batch cultures performed on waste peptones. Such organic nitrogen sources were shown to be an excellent low-cost substrate for microbial H, saving more than 50% of the nutrient costs.
authors.affiliation: Grupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain. jvazquez@iim.csic.es.
authors.forename: José A
authors.initials: JA
authors.lastname: Vázquez
authors.affiliation: Departamento de Química Analítica y Alimentaria, Facultad de Ciencias de Ourense (Universidad de Vigo), Campus As Lagoas s/n, Ourense-32004 Galicia, Spain. pastrana@uvigo.es.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Pastrana
authors.affiliation: Servicio de Instrumentación Científica (SICIM), Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain. cpineiro@iim.csic.es.
authors.forename: Carmen
authors.initials: C
authors.lastname: Piñeiro
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal. jateixeira@deb.uminho.pt.
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.affiliation: Grupo de Bioquímica de Alimentos, Instituto de Investigacións Mariñas (IIM-CSIC), r/Eduardo Cabello, 6. Vigo-36208 Galicia, Spain. ricardo@iim.csic.es.
authors.forename: Ricardo I
authors.initials: RI
authors.lastname: Pérez-Martín
authors.affiliation: Departamento de Química Analítica y Alimentaria, Facultad de Ciencias de Ourense (Universidad de Vigo), Campus As Lagoas s/n, Ourense-32004 Galicia, Spain. sabelara@uvigo.es.|Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal. sabelara@uvigo.es.
authors.forename: Isabel R
authors.initials: IR
authors.lastname: Amado
mesh_terms: D000818:Animals; D003470:Culture Media; D004284:Dogfish / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010461:Peptones / Q000378:metabolism*; D018502:Streptococcus equi / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010461:Peptones; D006820:Hyaluronic Acid; D009584:Nitrogen
keywords: Scyliorhinus canicula by-products; Streptococcus zooepidemicus; hyaluronic acid production; logistic equation; marine peptones; viscera waste valorization