title: The Native Plasmid pML21 Plays a Role in Stress Tolerance in Enterococcus faecalis ML21, as Analyzed by Plasmid Curing Using Plasmid Incompatibility.
abstract: To investigate the role of the native plasmid pML21 in Enterococcus faecalis ML21's response to abiotic stresses, the plasmid pML21 was cured based on the principle of plasmid incompatibility and segregational instability, generating E. faecalis mutant strain ML0. The mutant and the wild strains were exposed to abiotic stresses: bile salts, low pH, H2O2, ethanol, heat, and NaCl, and their survival rate was measured. We found that curing of pML21 lead to reduced tolerance to stress in E. faecalis ML0, especially oxidative and osmotic stress. Complementation analysis suggested that the genes from pML21 played different role in stress tolerance. The result indicated that pML21 plays a role in E. faecalis ML21's response to abiotic stresses.
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Fang-Lei
authors.initials: FL
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chen
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Feng
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.affiliation: Chinese National Egg Engineering Research Center, Beijing, 102115, People's Republic of China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Lu
authors.affiliation: College of Agriculture and Biotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Ma
authors.affiliation: Key Laboratory of Functional Dairy Science of Chinese Ministry of Education and Municipal Government of Beijing, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road Haidian District, Beijing, 100083, People's Republic of China. swchen@cau.edu.cn.
authors.forename: Shang-Wu
authors.initials: SW
authors.lastname: Chen
mesh_terms: D000222:Adaptation, Physiological; D013293:Enterococcus faecalis / Q000254:growth & development / Q000502:physiology*; D010957:Plasmids; D013312:Stress, Physiological
keywords: Enterococcus faecalis; Incompatibility; Native plasmid; Plasmid curing; Stress tolerance