title: Flavones Inhibit the Activity of AKR1B10, a Promising Therapeutic Target for Cancer Treatment.
abstract: AKR1B10 is an NADPH-dependent reductase that plays an important function in several physiological reactions such as the conversion of retinal to retinol, reduction of isoprenyl aldehydes, and biotransformation of procarcinogens and drugs. A growing body of evidence points to the important role of the enzyme in the development of several types of cancer (e.g., breast, hepatocellular), in which it is highly overexpressed. AKR1B10 is regarded as a therapeutic target for the treatment of these diseases, and potent and specific inhibitors may be promising therapeutic agents. Several inhibitors of AKR1B10 have been described, but the area of natural plant products has been investigated sparingly. In the present study almost 40 diverse phenolic compounds and alkaloids were examined for their ability to inhibit the recombinant AKR1B10 enzyme. The most potent inhibitors-apigenin, luteolin, and 7-hydroxyflavone-were further characterized in terms of IC50, selectivity, and mode of action. Molecular docking studies were also conducted, which identified putative binding residues important for the interaction. In addition, cellular studies demonstrated a significant inhibition of the AKR1B10-mediated reduction of daunorubicin in intact cells by these inhibitors without a considerable cytotoxic effect. Although these compounds are moderately potent and selective inhibitors of AKR1B10, they constitute a new structural type of AKR1B10 inhibitor and may serve as a template for the development of better inhibitors.
authors.forename: Lucie
authors.initials: L
authors.lastname: Zemanova
authors.forename: Jakub
authors.initials: J
authors.lastname: Hofman
authors.forename: Eva
authors.initials: E
authors.lastname: Novotna
authors.affiliation: Department of Chemistry, Faculty of Science, University of Hradec Kralove , Rokitanskeho 62, 500 03 Hradec Kralove, Czech Republic.
authors.forename: Kamil
authors.initials: K
authors.lastname: Musilek
authors.forename: Tereza
authors.initials: T
authors.lastname: Lundova
authors.forename: Jana
authors.initials: J
authors.lastname: Havrankova
authors.forename: Anna
authors.initials: A
authors.lastname: Hostalkova
authors.forename: Jakub
authors.initials: J
authors.lastname: Chlebek
authors.forename: Lucie
authors.initials: L
authors.lastname: Cahlikova
authors.forename: Vladimír
authors.initials: V
authors.lastname: Wsol
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors / Q000187:drug effects*; D000074408:Aldo-Keto Reductases; D047310:Apigenin / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000737:chemistry; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D045325:HCT116 Cells; D006801:Humans; D047311:Luteolin / Q000494:pharmacology; D008968:Molecular Conformation; D015394:Molecular Structure; D009369:Neoplasms / Q000188:drug therapy*
chemical_list: D004791:Enzyme Inhibitors; D047309:Flavones; D005419:Flavonoids; D047310:Apigenin; C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D047311:Luteolin; C429845:7-hydroxyflavone; D003630:Daunorubicin