title: EUCAST recommendations for antimicrobial susceptibility testing applied to the three main Campylobacter species isolated in humans.
abstract: Antimicrobial susceptibility testing of Campylobacter isolates is of great importance for treatment options especially in systemic diseases. The European Committee for Antimicrobial Susceptibility Testing (EUCAST) recently proposed epidemiological cut-offs (ECOFFs) for a limited number of antimicrobial compounds and for Campylobacter jejuni and Campylobacter coli only. In the present study, the EUCAST method was used after minor modifications to define antimicrobial susceptibility patterns for, 1997 C. jejuni, 419 C. coli and 100 Campylobacter fetus strains received at the French National Reference Center for Campylobacters and Helicobacters. Our results show that the ECOFFs defined by EUCAST for tetracycline and ciprofloxacin can be used for C. jejuni and C. coli. The same ECOFF can be used for erythromycin for the three species. The C. jejuni and C. coli ECOFFs for ciprofloxacin however cannot be applied to C. fetus. We also provide data to categorise two 2 β-lactams of interest for systemic diseases, ampicillin and amoxicillin+clavulanate, for the three species.
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Sifré
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France.
authors.forename: Ben Amor
authors.initials: BA
authors.lastname: Salha
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France.
authors.forename: Astrid
authors.initials: A
authors.lastname: Ducournau
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Floch
authors.affiliation: Centre Hospitalier du Pays d'Aix, Service de diagnostic biologique des maladies infectieuses, Aix en Provence, France.
authors.forename: Hubert
authors.initials: H
authors.lastname: Chardon
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Mégraud
authors.affiliation: Univ. Bordeaux, National Reference Center for Campylobacters and Helicobacters, Laboratoire de Bactériologie, Bordeaux, France; CHU de Bordeaux, 33076 Bordeaux, France. Electronic address: philippe.lehours@u-bordeaux.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lehours
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002167:Campylobacter / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000188:drug therapy / Q000382:microbiology*; D005060:Europe; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial susceptibility testing; Campylobacter; Disk diffusion; EUCAST; MIC