title: DNA barcoding identification of Dermestidae species.
abstract: Dermestidae species are important stored product insects. The traditional morphological identification has its limitation. The purpose of the study is to explore the effectiveness of identification by DNA barcoding technology in Dermestidae. The COI gene sequences of 39 samples from 17 species of Dermestidae were analyzed, and we evaluated the identification ability of the sequences by the tree building method and the distance evaluation method in this study. The COI sequences whose length was 356 bp had a large difference between intra-specific and inter-specific and a significant barcoding gap. The success rate of identification was 92.5%. The results showed a certain feasibility to identify the most species of Dermestidae by this segment of COI.
authors.affiliation: a Lab for Prevention and Control of Alienpests, Suzhou Entry Exit Inspection and Quarantine Bureau , Suzhou, Jiangsu Province , PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Sizhu
authors.affiliation: b Gold Mantis School of Architecture and Urban Environment of Soochow University , Suzhou, Jiangsu Province , PR China , and.
authors.forename: Li
authors.initials: L
authors.lastname: Jialin
authors.affiliation: b Gold Mantis School of Architecture and Urban Environment of Soochow University , Suzhou, Jiangsu Province , PR China , and.
authors.forename: Wei
authors.initials: W
authors.lastname: Yihan
authors.affiliation: a Lab for Prevention and Control of Alienpests, Suzhou Entry Exit Inspection and Quarantine Bureau , Suzhou, Jiangsu Province , PR China.
authors.forename: Gao
authors.initials: G
authors.lastname: Yuan
authors.affiliation: a Lab for Prevention and Control of Alienpests, Suzhou Entry Exit Inspection and Quarantine Bureau , Suzhou, Jiangsu Province , PR China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Guohui
authors.affiliation: c Lab for Plant Quarantine, Jiangsu Entry-Exit Inspection and Quarantine Bureau , Nanjing, Jiangsu Province , PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiaojun
authors.affiliation: a Lab for Prevention and Control of Alienpests, Suzhou Entry Exit Inspection and Quarantine Bureau , Suzhou, Jiangsu Province , PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yunfang
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D058893:DNA Barcoding, Taxonomic; D030541:Databases, Genetic; D003576:Electron Transport Complex IV / Q000145:classification / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004247:DNA; D003576:Electron Transport Complex IV
keywords: COI gene; DNA barcoding; Dermestidae; genetic distance; species identification