title: Clinical usefulness of WT1 mRNA expression in bone marrow detected by a new WT1 mRNA assay kit for monitoring acute myeloid leukemia: a comparison with expression of WT1 mRNA in peripheral blood.
abstract: We have previously shown the clinical usefulness of Wilms' tumor 1 gene (WT1) mRNA expression in peripheral blood (PB) as a minimal residual disease (MRD) monitoring marker in 191 acute myeloid leukemia (AML) patients using the WT1 mRNA assay kit "Otsuka" (Otsuka Pharmaceutical Co., Ltd.; "former kit"). In contrast, the usefulness of WT1 mRNA expression in bone marrow (BM) has been investigated in only a limited number of subjects using former kit. Following that previous study, a next-generation kit, WT1 mRNA assay kit II "Otsuka" (Otsuka Pharmaceutical Co., Ltd.; "new kit") has been newly developed. In the present study, we aimed to evaluate the performance of the new kit and to investigate the clinical usefulness of WT1 mRNA expression in BM. The PB and BM were collected on the same day from 164 blood disease patients, including 118 AML patients. WT1 mRNA expression was determined using the new and former kits and the values obtained were compared. The performance of new kit was shown to be equivalent to that of former kit. As reported in PB, WT1 mRNA expression in BM was found to be a useful marker for monitoring disease status as well as for a diagnosis of early stage relapse in AML patients.
authors.affiliation: Division of Hematology, Ichinomiya Municipal Hospital, 2-2-22 Bunkyo Ichinomiya, Aichi, 491-8558, Japan. kk-kitamura@nifty.com.
authors.forename: Kunio
authors.initials: K
authors.lastname: Kitamura
authors.affiliation: Division of Hematology, Ichinomiya Municipal Hospital, 2-2-22 Bunkyo Ichinomiya, Aichi, 491-8558, Japan.|Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Aichi, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Division of Hematology, Tokyo Metropolitan Ohtsuka Hospital, Tokyo, Japan.|Department of Hematology, Kanazawa University Hospital, Ishikawa, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Ishiyama
authors.affiliation: Division of Hematology, Tokyo Metropolitan Ohtsuka Hospital, Tokyo, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyawaki
authors.affiliation: Department of Hematology, Japanese Red Cross Medical Center, Tokyo, Japan.
authors.forename: Kanji
authors.initials: K
authors.lastname: Miyazaki
authors.affiliation: Department of Hematology, Japanese Red Cross Medical Center, Tokyo, Japan.
authors.forename: Kenshi
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Department of Hematology and Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masaie
authors.affiliation: Division of Hematology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Okada
authors.affiliation: Division of Hematology, Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ogawa
authors.affiliation: Department of Hematology, Sapporo Hokuyu Hospital, Sapporo, Japan.
authors.forename: Kiyotoshi
authors.initials: K
authors.lastname: Imai
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Aichi, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kiyoi
authors.affiliation: Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Aichi, Japan.|National Hospital Organization Nagoya Medical Center, Aichi, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
authors.affiliation: Department of Hematology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Yokoyama
authors.affiliation: Department of Hematology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.affiliation: Department of Hematology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Hata
authors.affiliation: Department of Hematology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: Department of Hematology and Rheumatology, Nihon University School of Medicine, Itabashi Hospital, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hatta
authors.affiliation: Department of Hematology and Rheumatology, Nihon University School of Medicine, Itabashi Hospital, Tokyo, Japan.|Division of Hematology, International University of Health and Welfare Shioya Hospital, Tochigi, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Takeuchi
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nannya
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Mineo
authors.initials: M
authors.lastname: Kurokawa
authors.affiliation: Department of Haematology/Oncology, Transfusion and Haemapheresis Center, Kurashiki Central Hospital, Okayama, Japan.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: Diagnostic Division, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koga
authors.affiliation: Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: The Japanese Association of Medical Sciences, Tokyo, Japan.
authors.forename: Fumimaro
authors.initials: F
authors.lastname: Takaku
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001853:Bone Marrow; D005260:Female; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis* / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D025721:WT1 Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D011933:Reagent Kits, Diagnostic; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Acute myeloid leukemia; Bone marrow; Myelodysplastic syndromes; One step real-time RT-PCR; WT1 mRNA