title: (E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression.
abstract: We investigated the potential melanogenic effect of compounds from Zingiber cassumunar Roxb. Our data revealed that chloroform-soluble extract of Z. cassumunar enhanced melanin synthesis in B16F10 melanoma cells. Among the components of the chloroform extract, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) increased melanogenesis in both B16F10 cells and human primary melanocytes. In B16F10 cells, DMPB enhanced the activation of ERK and p38, and the level of tyrosinase. Although the level of microphthalmia-associated transcription factor was unchanged in DMPB-treated B16F10 cells, DMPB increased levels and nuclear localization of upstream stimulating factor-1 (USF1). Consistently, DMPB-mediated melanin synthesis was diminished in USF1-knockdown cells. Furthermore, DMPB induced hyperpigmentation in brown guinea pigs in vivo. Together, these data suggest that DMPB may promote melanin synthesis via USF1 dependent fashion and could be used as a clinical therapeutic agent against hypopigmentation-associated diseases.
authors.affiliation: Department of Life Sciences, the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul, Korea.
authors.forename: Jisu
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Life Sciences, the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul, Korea.
authors.forename: Heesung
authors.initials: H
authors.lastname: Chung
authors.affiliation: Department of Dermatology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: Seung Hyun
authors.initials: SH
authors.lastname: Bang
authors.affiliation: The Global Top5 Research Program, College of Pharmacy, Ewha Womans University, Seoul, Korea.
authors.forename: Ah-Reum
authors.initials: AR
authors.lastname: Han
authors.affiliation: The Global Top5 Research Program, College of Pharmacy, Ewha Womans University, Seoul, Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Seo
authors.affiliation: Department of Dermatology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Chang
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Ewha Medical Research Center, Ewha Womans University School of Medicine, Seoul, Korea.
authors.forename: Duk-Hee
authors.initials: DH
authors.lastname: Kang
authors.affiliation: Department of Life Sciences, the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul, Korea.
authors.forename: Eok-Soo
authors.initials: ES
authors.lastname: Oh
mesh_terms: D000818:Animals; D000440:Butanols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D020939:Zingiber officinale / Q000737:chemistry* / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D059625:Liquid-Liquid Extraction; D008543:Melanins / Q000378:metabolism*; D008544:Melanocytes / Q000166:cytology / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010858:Pigmentation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051720:Upstream Stimulatory Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000440:Butanols; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D034741:RNA, Small Interfering; D051720:Upstream Stimulatory Factors; C497183:Usf1 protein, mouse; C053776:4-(3',4'-dimethoxyphenyl)but-3-en-1-ol; D014442:Monophenol Monooxygenase; D020928:Mitogen-Activated Protein Kinases; D000432:Methanol