title: Tailoring sub-micron PLGA particle release profiles via centrifugal fractioning.
abstract: Poly(D,L-lactic-co -glycolic) acid (PLGA)-based sub-micron particles are uniquely posed to overcome limitations of conventional drug delivery systems. However, tailoring cargo/payload release profiles from PLGA micro/nanoparticles typically requires optimization of the multi-parameter formulation, where small changes may cause drastic shifts in the resulting release profiles. In this study, we aimed to establish whether refining the average diameter of sub-micron particle populations after formulation alters protein release profiles. PLGA particles were first produced via double emulsion-solvent evaporation method to encapsulate bovine serum albumin. Particles were then subjected to centrifugal fractioning protocols varying in both spin time and force to determine encapsulation efficiency and release profile of differently sized populations that originated from a single batch. We found the average particle diameter was related to marked alterations in encapsulation efficiencies (range: 36.4-49.4%), burst release (range: 15.8-49.1%), and time for total cargo release (range: 38-78 days). Our data corroborate previous reports relating PLGA particle size with such release characteristics, however, this is the first study, to our knowledge, to directly compare particle population size while holding all formulation parameters constant. In summary, centrifugal fractioning to selectively control the population distribution of sub-micron PLGA particles represents a feasible tool to tailor release characteristics. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A 104A: 688-696, 2016.
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Dutta
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.
authors.forename: Mariama
authors.initials: M
authors.lastname: Salifu
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.|Barrow Brain Tumor Research Center, Barrow Neurological Institute, Phoenix, Arizona.
authors.forename: Rachael W
authors.initials: RW
authors.lastname: Sirianni
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Stabenfeldt
mesh_terms: D000818:Animals; D002417:Cattle; D002498:Centrifugation / Q000379:methods*; D016503:Drug Delivery Systems; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013997:Time Factors
chemical_list: D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine
keywords: PLGA particles; centrifugal fractioning; protein encapsulation; release profile