title: Monitoring of Fasciola Species Contamination in Water Dropwort by cox1 Mitochondrial and ITS-2 rDNA Sequencing Analysis.
abstract: Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 35015, Korea.
authors.forename: In-Wook
authors.initials: IW
authors.lastname: Choi
authors.affiliation: Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Korea.
authors.forename: Hwang-Yong
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
authors.forename: Juan-Hua
authors.initials: JH
authors.lastname: Quan
authors.affiliation: Microbial Safety Team, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Korea.
authors.forename: Jae-Gee
authors.initials: JG
authors.lastname: Ryu
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 35015, Korea.
authors.forename: Rubing
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 35015, Korea.
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005210:Fasciola hepatica / Q000235:genetics* / Q000302:isolation & purification*; D007723:Korea; D008969:Molecular Sequence Data; D031050:Oenanthe / Q000469:parasitology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV
keywords: DNA sequencing analysis; Fasciola species; ITS-2; cox1; water dropwort