title: Protein and messenger RNA expression of interleukin 1 system members in bovine ovarian follicles and effects of interleukin 1β on primordial follicle activation and survival in vitro.
abstract: This study aimed to investigate the expression of interleukin 1 (IL-1) system members (proteins and messenger RNA of ligands and receptors) and its distribution in ovarian follicles of cyclic cows and to evaluate the effects of IL-1β on the survival and activation of primordial follicles in vitro. The ovaries were processed for localization of IL-1 system in preantral and antral follicles by immunohistochemical, real-time polymerase chain reaction, and Western blot analysis. For in vitro studies, ovarian fragments were cultured in α-MEM(+) supplemented with IL-1β (0, 1, 10, 50, or 100 ng/mL), and after 6 d, the cultured tissues were processed for histologic analysis. Immunohistochemical results showed that the IL-1 system proteins IL-1β, IL-1RA, IL-1RI, and IL-1RII were detected in the cytoplasm of oocytes and granulosa cells from all follicular categories and theca cells of antral follicles. Variable levels of messenger RNA for the IL-1 system members were observed at different stages of development. After 6 d of culture, the presence of IL-1β (10 or 50 ng/mL) was effective in maintaining the percentage of normal follicles and in promoting primordial follicle activation. In conclusion, IL-1 system members are differentially expressed in ovarian follicles according to their stage of development. Moreover, IL-1β promotes the development of primordial follicles. These results indicate an important role of the IL-1 system in the regulation of bovine folliculogenesis.
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: J R S
authors.initials: JR
authors.lastname: Passos
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: J J N
authors.initials: JJ
authors.lastname: Costa
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: E V
authors.initials: EV
authors.lastname: da Cunha
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: A W B
authors.initials: AW
authors.lastname: Silva
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: R P
authors.initials: RP
authors.lastname: Ribeiro
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: G B
authors.initials: GB
authors.lastname: de Souza
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: P A A
authors.initials: PA
authors.lastname: Barroso
authors.affiliation: Faculty of Veterinary Medicine, University Veterinary Hospital, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: A M P
authors.initials: AM
authors.lastname: Dau
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil.
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.affiliation: Faculty of Veterinary Medicine, University Veterinary Hospital, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: P B D
authors.initials: PB
authors.lastname: Gonçalves
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: van den Hurk
authors.affiliation: Faculty of Medicine, Biotechnology Nucleus of Sobral-NUBIS, Federal University of Ceara, Sobral, CE CEP 62042-280, Brazil. Electronic address: roberto_viana@yahoo.com.
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000502:physiology*; D005260:Female; D006107:Granulosa Cells / Q000737:chemistry; D007150:Immunohistochemistry / Q000662:veterinary; D053590:Interleukin 1 Receptor Antagonist Protein / Q000032:analysis / Q000235:genetics; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D053583:Interleukin-1beta / Q000032:analysis / Q000235:genetics / Q000494:pharmacology*; D009865:Oocytes / Q000737:chemistry; D006080:Ovarian Follicle / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D053574:Receptors, Interleukin-1 Type II / Q000032:analysis / Q000235:genetics; D013799:Theca Cells / Q000737:chemistry
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D053583:Interleukin-1beta; D012333:RNA, Messenger; D053574:Receptors, Interleukin-1 Type II
keywords: Bovine; Gene expression; Interleukin 1 system; Primordial follicle