title: Predictive value of E-cadherin and Ep-CAM in cervical lymph node metastasis of supraglottic larynx carcinoma.
abstract: OBJECTIVE
  To evaluate the value of E-cadherin and epithelial cell adhesion molecule (Ep-CAM) expression in laryngeal biopsy materials for predicting cervical lymph node metastasis in patients with supraglottic laryngeal carcinoma.
  
  
  METHODS
  All patients participating in the study were selected from among the surgically treated patients at the department of Otolaryngology, Head and Neck Surgery, Erciyes University School of Medicine between 1991 and 2005. The study consisted of thirty patients who had pathologically metastatic lymph nodes (pN+ group) and 30 age-, sex-, T value- and differentiation matched patients without pathologically metastatic lymph nodes (pN0 group). Immunohistochemical studies were performed with E-cadherin and Ep-CAM antibodies on representative tumor sections collected from paraffin sections of laryngeal biopsy materials. The expression of E-cadherin and Ep-CAM was compared between the pN0 and pN+ groups. The association between immunostaining of E-cadherin and Ep-CAM was also evaluated.
  
  
  RESULTS
  There was no significant difference between the two groups in terms of E-cadherin and Ep-CAM expression. There was also a very poor agreement between the expression of E-cadherin and Ep-CAM.
  
  
  CONCLUSIONS
  Multi-institutional and multidisciplinary immunohistochemical studies conducted with standardized methodology and also with more patient participation may help to obtain more specific results.
authors.affiliation: Department of ENT, Kayseri Training and Research Hospital, Kayseri, Turkey. Electronic address: dralibayram@gmail.com.
authors.lastname: Ali Bayram
authors.affiliation: Department of ENT, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: İmdat
authors.initials: İ
authors.lastname: Yüce
authors.affiliation: Department of ENT, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: Sedat
authors.initials: S
authors.lastname: Çağlı
authors.affiliation: Department of Pathology, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Canöz
authors.affiliation: Department of ENT, Erciyes University School of Medicine, Kayseri, Turkey.
authors.forename: Ercihan
authors.initials: E
authors.lastname: Güney
mesh_terms: D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D015820:Cadherins / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D007830:Larynx / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015820:Cadherins; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule