title: ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study.
abstract: In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.
authors.affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Kutuzov
authors.affiliation: N.N. Semenov Institute of Chemical Physics, RAS, Kosigin str. 4, Moscow, Russian Federation, 119991.|Chemistry Faculty, Moscow State University, Leninskie Gory 1-3, Moscow, Russian Federation, 119991.
authors.forename: Alexander
authors.initials: A
authors.lastname: Gulin
authors.affiliation: All-Russian Research Institute for Optical and Physical Measurements, Ozernaya 46, Moscow, Russian Federation, 119361.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lyaskovskiy
authors.affiliation: N.N. Semenov Institute of Chemical Physics, RAS, Kosigin str. 4, Moscow, Russian Federation, 119991.|Chemistry Faculty, Moscow State University, Leninskie Gory 1-3, Moscow, Russian Federation, 119991.|Moscow Institute of Physics and Technology State University, Institutskiy per. 9, Dolgoprudny, Moscow Region, Russian Federation, 141700.|Institute of Problems of Chemical Physics RAS, Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432.
authors.forename: Victor
authors.initials: V
authors.lastname: Nadtochenko
authors.affiliation: Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.
authors.forename: Georgy
authors.initials: G
authors.lastname: Maksimov
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000465:Algorithms; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism*; D008560:Membrane Fluidity / Q000187:drug effects; D008954:Models, Biological; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism*; D011896:Rana temporaria; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008051:Lipid Bilayers; D000255:Adenosine Triphosphate