title: The Effect of Schizandrol A-Induced DNA Methylation on SH-SY5YAB 1-40 Altered Neuronal Cell Line: A Potential Use in Alzheimer's Disease.
abstract: Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimer's disease (AD). The SH-SY5Y neuronal cell line treated with Aβ1-40 (5 μmol/L) protein is considered to be a model of AD. Hence the aim of this study was to examine the influence of Schizandrol A, a plant extract, on DNA methylation in SH-SY5Y cells exposed to Aβ1-40. Aβ1-40 were incubated with varying concentrations of Sehizandrol A to a final concentration of 1 (low), 3 (intermediate) or 9 μg/ml (high). Exposure of SH-SY5Y with Aβ1-40 reduced viability, and altered cellular morphology and mRNA expression of DNA methyltransferase (DNMT3A) and DNMT3B. Treatment with 1 or 3 μg/ml Sehizandrol A resulted in normal cell morphology as well as elevated cell number, enhanced viability, and increased mRNA expression of DNMT3A and DNMT3B compared to saline. However, an increase in Sehizandrol A to 9 μg/ml produced a fall in cell viability, as well as a decrease in mRNA DNMT3A and DNMT3B expression to control levels. Data demonstrated that Schizandrol A at 1 or 3 μg/ml improved cell morphological appearance and viability of Aβ1-40 injured SH-SY5Y cells by an enhanced DNA methylation pathway.
authors.affiliation: a Department of Anatomy , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a Department of Anatomy , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Huo
authors.affiliation: a Department of Anatomy , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Cai
authors.affiliation: b Medical Research Center of Biomedicine , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Guo
authors.initials: G
authors.lastname: Shao
authors.affiliation: c School of Health Sciences , University of Newcastle , Newcastle , Australia.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: a Department of Anatomy , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Zhi-ying
authors.initials: ZY
authors.lastname: Zhao
authors.affiliation: a Department of Anatomy , Baotou Medical College of Inner Mongolia University of Science and Technology , Inner Mongolia , China.
authors.forename: Zhan-jun
authors.initials: ZJ
authors.lastname: Yang
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D034242:Cyclooctanes / Q000494:pharmacology*; D019175:DNA Methylation / Q000187:drug effects*; D005786:Gene Expression Regulation; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016229:Amyloid beta-Peptides; D034242:Cyclooctanes; D017705:Lignans; D010446:Peptide Fragments; D011083:Polycyclic Compounds; D012333:RNA, Messenger; C078592:amyloid beta-protein (1-40); C011105:schizandrin