title: Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin.
abstract: 16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test.
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA patrick.t.mcgann4.ctr@mail.mil.
authors.forename: Patrick
authors.initials: P
authors.lastname: McGann
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Sarah
authors.initials: S
authors.lastname: Chahine
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Darius
authors.initials: D
authors.lastname: Okafor
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Ana C
authors.initials: AC
authors.lastname: Ong
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Rosslyn
authors.initials: R
authors.lastname: Maybank
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Yoon I
authors.initials: YI
authors.lastname: Kwak
authors.affiliation: Infectious Diseases Service, Walter Reed National Military Medical Center, Bethesda, Maryland, USA.
authors.forename: Kerry
authors.initials: K
authors.lastname: Wilson
authors.affiliation: Division of Bacterial & Rickettsial Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Zapor
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Emil
authors.initials: E
authors.lastname: Lesho
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Hinkle
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism*; D003982:Dibekacin / Q000031:analogs & derivatives* / Q000378:metabolism; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods*; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012359:tRNA Methyltransferases / Q000032:analysis* / Q000235:genetics
chemical_list: D000890:Anti-Infective Agents; D012336:RNA, Ribosomal, 16S; D003982:Dibekacin; D012359:tRNA Methyltransferases; C031986:arbekacin