title: Isolation and characterization of two novel psychrotrophic decabromodiphenyl ether-degrading bacteria from river sediments.
abstract: Decabromodiphenyl ether (BDE-209) is a brominated flame retardant and a priority contaminant. Currently, little information is available about its significance in the environment, specifically about its susceptibility to aerobic biotransformation at low temperature. In this work, five phylogenetically diverse BDE-209-degrading bacterial strains were isolated from river sediments of northern China. These strains were distributed among four different genera-Acinetobacter, Pseudomonas, Bacillus and Staphylococcus. All five isolates were capable of growing on BDE-209, among which two isolates show better growth. By detailed morphological, physiological, and biochemical characteristics and 16S rDNA sequence analysis, the two strains were identified and named as Staphylococcus haemolyticus LY1 and Bacillus pumilus LY2. The two bacteria can grow in mineral salt medium containing BDE-209 substrate across the temperatures ranging from 2.5 to 35 °C, with an optimum temperature of 25 °C which could be considered as psychrotrophs accordingly. The degradation experiment showed that more than 70.6 and 85.5 % of 0.5 mg/L BDE-209 were degraded and the highest mineralization efficiencies of 29.8 and 39.2 % were achieved for 0.5 mg/L BDE-209 by S. haemolyticus LY1 and B. pumilus LY2, respectively. To the best of our knowledge, this is the first demonstration for the biodegradation of BDE-209 by two psychrotrophic bacteria isolated from environment.
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Linqiong
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China. envly@hhu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China. 1223zhangwenlong@163.com.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Niu
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.affiliation: Key Laboratory of Integrated Regulation and Resource Development of Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Xikang Road #1, Nanjing, 210098, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000150:Acinetobacter / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D000069979:Bacillus pumilus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001711:Biotransformation; D002681:China; D004275:DNA, Ribosomal; D005411:Flame Retardants / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism*; D010802:Phylogeny; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D045483:Rivers / Q000382:microbiology; D041162:Staphylococcus haemolyticus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; C010902:decabromobiphenyl ether
keywords: Aerobic biodegradation; Bacillus pumilus; Decabromodiphenyl ether; Psychrotrophic bacteria; Staphylococcus haemolyticus