title: Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahliae infection in tobacco.
abstract: Verticillium wilt is a soil borne disease that can cause devastating losses to the production of many economically important crops. A Ve1 homologous gene responding to Verticillium dahliae infection was identified in Vitis vinifera cv. "HeiFeng" by semi-quantitative reverse transcription polymerase chain reaction and was designated as VvVe. The overexpression of VvVe in transgenic Nicotiana benthamiana plants significantly enhanced the resistance to isolate V991 of V. dahliae when compared with the wild type plants. The expressions of defense-related genes including the salicylic acid regulated gene pathogen-related 1 (PR1) but not PR2, the ethylene- and jasmonic acid-regulated genes ethylene response factor 1 (ERF1) and lipoxygenase (LOX) were significantly increased due to over expression of VvVe. And greater accumulation of active oxygen, callose and phenylalanine-ammonia lyase were observed in the leaves of transgenic VvVe tobacco plants than the wild type when under infection by V. dahliae. Moreover, the hypersensitive response mimicking cell death was exclusively occurred in the transgenic VvVe tobacco plants but not in the wild type. Taken together, the VvVe gene is a Ve1 like gene which involves in the signal cascade of salicylic acid, jasmonate, and ethylene defense pathways and enhances defense response to V. dahliae infection in the transgenic tobacco.
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Tang
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Tianzi
authors.initials: T
authors.lastname: Chen
authors.affiliation: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Xitie
authors.initials: X
authors.lastname: Ling
authors.affiliation: Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
authors.forename: Baolong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Electronic address: cyh@jaas.ac.cn.
authors.forename: Youhong
authors.initials: Y
authors.lastname: Chang
mesh_terms: D017343:Genes, Plant; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000382:microbiology*; D020172:Verticillium / Q000276:immunology* / Q000472:pathogenicity; D027843:Vitis / Q000235:genetics*
keywords: Disease resistance; Nicotiana benthamiana; Verticillium wilt; Vitis vinifera; VvVe