title: Sparstolonin B inhibits lipopolysaccharide-induced inflammation in 3T3-L1 adipocytes.
abstract: Sparstolonin B (SsnB), an isocoumarin compound isolated from the tubers of both Sparganium stoloniferum and Scirpus yagara, has been reported to have anti-inflammatory effects. However, whether SsnB has anti-inflammatory effects on LPS-stimulated 3T3-L1 adipocytes remains unclear. In this study, we investigated the effects of SsnB on adipocyte inflammation in 3T3-L1 adipocytes and anti-obesity properties in high fat diet (HFD)-induced obese rats. 3T3-L1 adipocytes were pretreated with SsnB 1h before LPS treatment. The expression of MCP-1, IL-6, TNF-α, and IL-10 were measured by qRT-PCR and ELISA. The expression of PPAR-γ, TLR4 and NF-κB were detected by western blotting. SsnB was administered to HFD-induced obese rats to confirm its effects in vivo. Our results showed that SsnB dose-dependently inhibited LPS-induced MCP-1, IL-6, and TNF-α production. SsnB was found to inhibit LPS-induced TLR4 expression and NF-κB activition. Furthermore, SsnB was found to activate PPAR-γ and the inhibitory effects of SsnB on MCP-1, IL-6, and TNF-α production can be reversed by PPAR-γ antagonist GW9662. In vivo, SsnB was found to reduce the body weight of rats fed with HFD. SsnB also inhibited the levels of serum triglyceride (TG) and cholesterol (TC) induced by HFD. In conclusion, the results suggested that SsnB could reduce HFD-induced obesity in rats and inhibited LPS-induced cytokines production in 3T3-L1 adipocytes by activating PPAR-γ.
authors.affiliation: Nephrology center of integrated traditional Chinese Medicine and Western Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Epidemiology and Medical Statistics, School of Public Health, Guangdong Medical College, Dongguan, Guangdong 523808, China.
authors.forename: Liangchang
authors.initials: L
authors.lastname: Xiu
authors.affiliation: The key laboratory of molecular biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Diao
authors.affiliation: Nephrology center of integrated traditional Chinese Medicine and Western Medicine, ZhuJiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Lianbo
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Endocrinology, ZhuJiang Hospital, Southern Medical University, Guangzhou ,510280 China. Electronic address: sunjiagz@163.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Sun
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000235:genetics; D059305:Diet, High-Fat / Q000009:adverse effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology* / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009765:Obesity / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D047495:PPAR gamma / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D006576:Heterocyclic Compounds, 4 or More Rings; D015850:Interleukin-6; D016328:NF-kappa B; D047495:PPAR gamma; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C561322:sparstolonin B; D016753:Interleukin-10
keywords: 3T3-L1 adipocyte; LPS; NF-κB; Sparstolonin B; TLR4