title: Ultrastructure of medial rectus muscles in patients with intermittent exotropia.
abstract: PURPOSE To study the ultrastructure of the medial rectus in patients with intermittent exotropia at different ages.PATIENTS AND METHODS The medial recti were harvested surgically from 20 patients with intermittent exotropia. Patients were divided into adolescent (age<18 years, n=10) and adult groups (age >18 years, n=10). The normal control group included five patients without strabismus and undergoing eye enucleation. Hematoxylin and eosin staining and transmission electron microscopy were used to visualize the medial recti. Western blot was used to determine the levels of myosin and actin.RESULTS Varying fiber thickness, atrophy, and misalignment of the medial recti were visualized under optical microscope in patients with exotropia. Electron microscopy revealed sarcomere destruction, myofilament disintegration, unclear dark and light bands, collagen proliferation, and fibrosis. The adolescent group manifested significantly higher levels of myosin and actin than the adult group (P<0.05).CONCLUSION Younger patients with intermittent exotropia show stronger contraction of the medial recti compared with older patients. Our findings suggest that childhood was the appropriate time for surgery as the benefit of the intervention was better than in adulthood.
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: Yao
authors.affiliation: Medical College of Soochow University, Suzhou, Jiangsu Province, China.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: P
authors.initials: P
authors.lastname: Lu
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D005099:Exotropia / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D046529:Microscopy, Electron, Transmission; D009218:Myosins / Q000378:metabolism; D009801:Oculomotor Muscles / Q000378:metabolism / Q000601:surgery / Q000648:ultrastructure*; D013508:Ophthalmologic Surgical Procedures; D055815:Young Adult
chemical_list: D000199:Actins; D009218:Myosins