title: Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity.
abstract: Vaccines blocking malaria parasites in the blood-stage diminish mortality and morbidity caused by the disease. Here, we isolated antigens from total parasite proteins by antibody affinity chromatography to test an immunization against lethal malaria infection in a murine model. We used the sera of malaria self-resistant ICR mice to lethal Plasmodium yoelii yoelii 17XL for purification of their IgGs which were subsequently employed to isolate blood-stage parasite antigens that were inoculated to immunize BALB/c mice. The presence of specific antibodies in vaccinated mice serum was studied by immunoblot analysis at different days after vaccination and showed an intensive immune response to a wide range of antigens with molecular weight ranging between 22 and 250 kDa. The humoral response allowed delay of the infection after the inoculation to high lethal doses of P. yoelii yoelii 17XL resulting in a partial protection against malaria disease, although final survival was managed in a low proportion of challenged mice. This approach shows the potential to prevent malaria disease with a set of antigens isolated from blood-stage parasites.
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Central Tehran Branch, Tehran 14676-86831, Iran.
authors.forename: Ali N
authors.initials: AN
authors.lastname: Kamali
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Department of Medicine and Surgery, Psychology, Preventive Medicine and Public Health and Medical Immunology and Microbiology, Faculty of Health Sciences, Universidad Rey Juan Carlos, Alcorcón, 28922 Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Marín-García
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Research Institute Hospital 12 de Octubre, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Isabel G
authors.initials: IG
authors.lastname: Azcárate
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Research Institute Hospital 12 de Octubre, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Puyet
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Research Institute Hospital 12 de Octubre, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Amalia
authors.initials: A
authors.lastname: Diez
authors.affiliation: Department of Biochemistry and Molecular Biology IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Ciudad Universitaria, 28040 Madrid, Spain ; Research Institute Hospital 12 de Octubre, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Bautista
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D008288:Malaria / Q000276:immunology / Q000517:prevention & control; D017780:Malaria Vaccines / Q000276:immunology; D051379:Mice; D010961:Plasmodium / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D017780:Malaria Vaccines