title: Single nucleotide polymorphisms in 5'-UTR of the SLC2A4 gene regulate solute carrier family 2 member 4 gene expression in visceral adipose tissue.
abstract: BACKGROUND
  The glucose uptake by numerous cells like adipocytes or skeletal muscle cells requires activation of specific proteins called glucose transporters. GLUT4 content and its activation as well as SLC2A4 gene expression level are decreased in patients with type 2 diabetes. The SLC2A4 gene expression rate might be modulated by genetic variances in UTRs. The relationship between two SNPs in 5'UTR and one SNP in 3'UTR and SLC2A4 gene expression rate in adipose tissue and lymphocytes has been evaluated.
  
  
  METHODS
  The study was performed on lymphocytes collected from 34 type 2 diabetic patients and equal number of controls. Visceral adipose tissue biopsies were collected from 15 patients with T2DM and from 24 controls.
  
  
  RESULTS
  SLC2A4 gene expression level was slightly lower within type 2 diabetic patients in both type of tissues. Furthermore, the negative correlation between SLC2A4 gene expression level in visceral adipose tissue and BMI has been noticed. The genotypes of two SNPs in 5'UTRs (rs5417 and rs5418) were correlated with lower mRNA amount of GLUT4 within recessive homozygotes belonging to control group.
  
  
  CONCLUSIONS
  Present results suggest the relationship between genetic variances within UTRs of SLC2A4 gene and gene expression rate. However, the relationship is tissue specific and is noticeable in adipose tissue of lean and healthy subjects.
authors.affiliation: Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Sklodowskiej-Curie 52, 50-369 Wroclaw, Poland. Electronic address: malgorzata.malodobra@umed.wroc.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Malodobra-Mazur
authors.affiliation: Department of Angiology, Hypertension and Diabetology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bednarska-Chabowska
authors.affiliation: First Department and Clinic of General, Gastroenterological and Endocrinological Surgery, Wroclaw Medical University, M. Curie-Skłodowskiej 66, 50-369 Wrocław, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Olewinski
authors.affiliation: Provincial Specialist Hospital in Wroclaw, Kamieńskiego 73 a, 51-124 Wroclaw, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Chmielecki
authors.affiliation: Department of Angiology, Hypertension and Diabetology, Wroclaw Medical University, Borowska 213, 50-556 Wroclaw, Poland.
authors.forename: Rajmund
authors.initials: R
authors.lastname: Adamiec
authors.affiliation: Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Sklodowskiej-Curie 52, 50-369 Wroclaw, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Dobosz
mesh_terms: D020121:5' Untranslated Regions; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D051275:Glucose Transporter Type 4 / Q000235:genetics*; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide
chemical_list: D020121:5' Untranslated Regions; D051275:Glucose Transporter Type 4; C495692:SLC2A4 protein, human
keywords: Lymphocytes; SLC2A4 gene; SNP; Type 2 diabetes; UTR; Visceral adipose tissue