title: Detection and Monitoring of Amyloid Fibrillation Using a Fluorescence "Switch-On" Probe.
abstract: Amyloid protein fibrillation is associated with a variety of neurodegenerative and other diseases, and their efficient detection and monitoring can greatly advance early diagnosis and therapy. Herein, we report a fluorescent "switch-on" probe for the reliable detection and monitoring of amyloid fibrils. The probe consists of a peptide component for binding with amyloid structure and a color component with an aggregation-induced green emission property. This probe is nonfluorescent in the presence of amyloid forming monomer protein/peptide, but fluorescence "switch-on" occurs after binding with amyloid fibrils. Compared to conventionally used thioflavin T, this probe offers a high signal-to-noise ratio, which is unaffected by the quencher ion/nanoparticle. The proposed new probe has been used for the detection and monitoring of amyloid fibrils produced by a wide variety of amyloid protein/peptides and can be extended for in vitro diagnostic applications.
authors.affiliation: Centre for Advanced Materials, Indian Association for the Cultivation of Science , Kolkata 700 032, India.
authors.forename: Nibedita
authors.initials: N
authors.lastname: Pradhan
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology , Shibpur, Howrah 711 103, India.
authors.forename: Debabrata
authors.initials: D
authors.lastname: Jana
authors.affiliation: Department of Chemistry, Indian Institute of Engineering Science and Technology , Shibpur, Howrah 711 103, India.
authors.forename: Binay K
authors.initials: BK
authors.lastname: Ghorai
authors.affiliation: Centre for Advanced Materials, Indian Association for the Cultivation of Science , Kolkata 700 032, India.
authors.forename: Nikhil R
authors.initials: NR
authors.lastname: Jana
mesh_terms: D000682:Amyloid / Q000032:analysis*; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D052160:Benzothiazoles; D002417:Cattle; D002645:Chickens; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D010455:Peptides; D013844:Thiazoles; C009462:thioflavin T; D001224:Aspartic Acid
keywords: Alzheimer’s; aggregation-induced emission; amyloid detection; amyloid fibril; fluorescence probe; tetraphenylethene