title: High-Efficiency Recognition and Identification of Disulfide Bonded Peptides in Rat Neuropeptidome Using Targeted Electron Transfer Dissociation Tandem Mass Spectrometry.
abstract: The main goal of the present study is to develop a method to recognize and identify endogenous intrachain disulfide bonded peptide, which are rarely sequenced in current peptidomics studies. In order to achieve highly efficient detection of these peptides in a neuropeptidome analysis, we alkylated the peptides, mined the raw mass spectrometry data, and then recognized the candidates of untreated disulfide bonded peptides from unalkylated peptide extracts. After removing more than 90% features, targeted electron transfer dissociation fragmentation was performed for detecting and fragmenting disulfide bonded peptides, and even most of them were present in low abundance in the original sample. Diverse endogenous disulfide bonded peptides were then detected and sequenced, opening up new perspectives for comprehensively understanding the response of a neuropeptidome.
authors.affiliation: Division of Biological Technology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Zhongshan Road 457, Dalian, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yu
authors.affiliation: Visual Cognition Laboratory, Department of Medicine, University of Fribourg , Chemin de Musee 5, Fribourg, CH-1700, Switzerland.
authors.forename: Abbas
authors.initials: A
authors.lastname: Khani
authors.affiliation: Shenyang Pharmaceutical University , Wenhua Road 103, Shenyang, China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Ye
authors.affiliation: Visual Cognition Laboratory, Department of Medicine, University of Fribourg , Chemin de Musee 5, Fribourg, CH-1700, Switzerland.
authors.forename: Filomena
authors.initials: F
authors.lastname: Petruzziello
authors.affiliation: Shenyang Pharmaceutical University , Wenhua Road 103, Shenyang, China.
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Gao
authors.affiliation: Division of Biological Technology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Zhongshan Road 457, Dalian, China.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Visual Cognition Laboratory, Department of Medicine, University of Fribourg , Chemin de Musee 5, Fribourg, CH-1700, Switzerland.
authors.forename: Gregor
authors.initials: G
authors.lastname: Rainer
mesh_terms: D000818:Animals; D004220:Disulfides / Q000737:chemistry*; D004583:Electrons; D009479:Neuropeptides / Q000032:analysis* / Q000737:chemistry*; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D004220:Disulfides; D009479:Neuropeptides