title: Pattern of cytokine and chemokine production by THP-1 derived macrophages in response to live or heat-killed Mycobacterium bovis bacillus Calmette-Guérin Moreau strain.
abstract: Tuberculosis has great public health impact with high rates of mortality and the only prophylactic measure for it is the Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccine. The present study evaluated the release of cytokines [interleukin (IL)-1, tumour necrosis factor and IL-6] and chemokines [macrophage inflammatory protein (MIP)-1α and MIP-1β] by THP-1 derived macrophages infected with BCG vaccine obtained by growing mycobacteria in Viscondessa de Moraes Institute medium medium (oral) or Sauton medium (intradermic) to compare the effects of live and heat-killed (HK) mycobacteria. Because BCG has been reported to lose viability during the lyophilisation process and during storage, we examined whether exposing BCG to different temperatures also triggers differences in the expression of some important cytokines and chemokines of the immune response. Interestingly, we observed that HK mycobacteria stimulated cytokine and chemokine production in a different pattern from that observed with live mycobacteria.
authors.affiliation: Laboratório de Imunologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Periela da Silva
authors.initials: Pda S
authors.lastname: Sousa-Vasconcelos
authors.affiliation: Departamento de Pesquisa, Fundação Ataulpho Paiva, Rio de Janeiro, RJ, Brasil.
authors.forename: Wellington da Silva
authors.initials: Wda S
authors.lastname: Seguins
authors.affiliation: Laboratório de Imunologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Eduardo de Souza
authors.initials: Ede S
authors.lastname: Luz
authors.affiliation: Laboratório de Imunologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Rosa Teixeira de
authors.initials: RT
authors.lastname: Pinho
mesh_terms: D002460:Cell Line; D054405:Chemokine CCL3 / Q000378:metabolism; D054407:Chemokine CCL4 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D050296:Microbial Viability / Q000276:immunology*; D009163:Mycobacterium bovis / Q000145:classification* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015164:Vaccines, Inactivated
chemical_list: D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D015164:Vaccines, Inactivated