title: Rapid method for protein quantitation by Bradford assay after elimination of the interference of polysorbate 80.
abstract: Bradford assay is one of the most common methods for measuring protein concentrations. However, some pharmaceutical excipients, such as detergents, interfere with Bradford assay even at low concentrations. Protein precipitation can be used to overcome sample incompatibility with protein quantitation. But the rate of protein recovery caused by acetone precipitation is only about 70%. In this study, we found that sucrose not only could increase the rate of protein recovery after 1 h acetone precipitation, but also did not interfere with Bradford assay. So we developed a method for rapid protein quantitation in protein drugs even if they contained interfering substances.
authors.affiliation: Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, China; Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China.
authors.forename: Haiming
authors.initials: H
authors.lastname: Wei
authors.affiliation: Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, China; Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Sun
authors.affiliation: Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, China; Hefei National Laboratory for Physical Sciences at Microscale, Hefei, Anhui 230027, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027, China. Electronic address: zhxdd@ustc.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001548:Benzalkonium Compounds / Q000737:chemistry; D002417:Cattle; D011232:Chemical Precipitation; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003124:Colorimetry; D003902:Detergents / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D001548:Benzalkonium Compounds; D003902:Detergents; D011136:Polysorbates; D011506:Proteins; D012710:Serum Albumin, Bovine; D014238:Trichloroacetic Acid; C015163:dimethyldodecylbenzylammonium
keywords: Bradford assay; Protein precipitation; Sucrose