title: Immune-mediated Skin Inflammation is Similar in Severe Atopic Dermatitis Patients With or Without Filaggrin Mutation.
abstract: Inflammatory cytokines can impair the skin barrier, but the question as to whether barrier alterations affect keratinocyte immune responses remains unanswered. The aim of this study was to investigate whether immune-mediated skin inflammation differs between severe atopic dermatitis patients with or without filaggrin mutation. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified by immunohistochemistry. The results were confirmed by quantitative PCR analyses. No significant differences were found between the 2 patient groups. Expression of atopic dermatitis-specific cytokines showed significant correlation with histological severity. These findings suggest that the immune-mediated skin inflammation (represented by keratinocyte-derived factors, T cell and dendritic cell counts) is similar in the 2 patient groups with severe atopic dermatitis, and that immune activation is connected to the severity of the disease rather than to the origin of barrier alterations.
authors.affiliation: Division of Dermatological Allergology, Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt 98, Debrecen, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Dajnoki
authors.forename: Gabriella
authors.initials: G
authors.lastname: Béke
authors.forename: Gábor
authors.initials: G
authors.lastname: Mócsai
authors.forename: Anikó
authors.initials: A
authors.lastname: Kapitány
authors.forename: Krisztián
authors.initials: K
authors.lastname: Gáspár
authors.forename: Krisztina
authors.initials: K
authors.lastname: Hajdu
authors.forename: Gabriella
authors.initials: G
authors.lastname: Emri
authors.forename: Bence
authors.initials: B
authors.lastname: Nagy
authors.forename: Ilona
authors.initials: I
authors.lastname: Kovács
authors.forename: Lívia
authors.initials: L
authors.lastname: Beke
authors.forename: Balázs
authors.initials: B
authors.lastname: Dezső
authors.forename: Andrea
authors.initials: A
authors.lastname: Szegedi
mesh_terms: D000293:Adolescent; D001706:Biopsy; D054425:Chemokine CCL27 / Q000276:immunology; D002648:Child; D016207:Cytokines / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000276:immunology*; D000091344:Filaggrin Proteins; D005838:Genotype; D006801:Humans; D007113:Immunity, Innate; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology; D000067596:Interleukin-33 / Q000276:immunology; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000276:immunology*; D015603:Keratinocytes / Q000276:immunology; D018655:Lymphocyte Count; D009154:Mutation; D016133:Polymerase Chain Reaction; D055815:Young Adult; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D054425:Chemokine CCL27; D016207:Cytokines; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D000067596:Interleukin-33; D007381:Intermediate Filament Proteins; D000094632:Thymic Stromal Lymphopoietin