title: Selective separation and determination of isoproterenol on thin layers of bismuth silicate ion-exchanger.
abstract: A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate, a synthetic inorganic ion exchanger as adsorbent in thin layer chromatography (TLC). A mixture of methanol and 0.1 mol/L formic acid (3:7, v/v) was employed as the mobile phase. The development time was 32 min. The quantitative measurement were performed with a Camag TLC Scanner-3 at wavelength (λ) of 410 nm. The isoproterenol recovery in this procedure was 98.9%. The linear correlation coefficient was greater than 0. 987 1 and the relative standard deviation (RSD) was less than 0.94. The limit of detection (LOD) and limit of quantification ( LOQ) were 7.7 x 10(-7) mol/L and 3.85 x 10(-6) mol/L, respectively. This method has been applied in the determination of isoproterenol in dosage forms and in biological fluids.
authors.lastname: Ghoulipour Vanik
authors.lastname: Hassankhani-Majd Zahra
mesh_terms: D001729:Bismuth; D002855:Chromatography, Thin Layer; D007545:Isoproterenol / Q000302:isolation & purification*; D057230:Limit of Detection; D017640:Silicates
chemical_list: D017640:Silicates; D007545:Isoproterenol; D001729:Bismuth