title: First report of the toxigenic Nitzschia navis-varingica (Bacillariophyceae) isolated from Tebrau Straits, Johor, Malaysia.
abstract: Screening of the occurrence of potentially toxic diatoms was carried out at two sites of cage cultures in Tebrau Straits, Johor. Phytoplankton samples from Sungai Pendas and Teluk Sengat were collected using a 20 μm mesh plankton net and salinity was recorded in-situ. Nitzschia and Pseudo-nitzschia cells were isolated and established into clonal cultures. All cultures were tested for domoic acid using HPLC-UV analysis and verified by LC-MS analysis. Three Nitzschia spp. and one Pseudo-nitzschia sp. were identified from these locations. Toxic and non-toxic strains of Nitzschia navis-varingica are found at the cage culture areas. Cellular toxin content in the toxic strain of N. navis-varingica is 1.8 pg cell(-1). This is a new record from Malaysia and this species was isolated from estuarine water with salinity 28 PSU. The discovery of toxic Nitzschia species in Tebrau Straits indicates the potential for domoic acid accumulation in seafood.
authors.affiliation: School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia. Electronic address: sue_0586@yahoo.com.my.
authors.forename: S N P
authors.initials: SN
authors.lastname: Suriyanti
authors.affiliation: School of Environmental and Natural Resources Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.
authors.forename: Gires
authors.initials: G
authors.lastname: Usup
mesh_terms: D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D017377:Diatoms / Q000145:classification* / Q000302:isolation & purification; D063366:Estuaries; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D008296:Malaysia; D008387:Marine Toxins / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry
chemical_list: D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid
keywords: Amnesic shellfish poisoning; Benthic diatom; Domoic acid; Marine; Toxic