title: Alkyl hydroperoxide reductase from Bacillus aquimaris MKSC 6.2 protects Esherichia coli from oxidative stress.
abstract: Alkyl hydroperoxide reductase genes (ahpCF) from the soft coral associated Bacillus aquimaris MKSC6.2 have been isolated. The cloned 546 bp ahpC gene encodes a 181 amino acid residues polypeptide. The AhpC belongs to typical 2-Cys peroxiredoxin (Prx) containing conserved peroxidatic cysteine residue (C46 ) required for hydroperoxide reduction and conserved resolving cysteine (C166 ). The isolated 1530 bp ahpF gene encodes a polypeptide of 509 amino acid residues with two conserved C128 HNC131 and C337 PHC340 catalytic residues required for reduction of oxidized-AhpC during catalytic turnover. A survival study with Escherichia coli showed that overexpression of AhpC and AhpF resulted in a total protection against 0.16 mM t-butyl hydroperoxide.
authors.affiliation: Division of Biochemistry Research, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia.|Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Dessy
authors.initials: D
authors.lastname: Natalia
authors.affiliation: Division of Biochemistry Research, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Ozi
authors.initials: O
authors.lastname: Jumadila
authors.affiliation: Division of Biochemistry Research, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Irika Devi
authors.initials: ID
authors.lastname: Anggraini
authors.affiliation: Division of Biochemistry Research, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Febrina
authors.initials: F
authors.lastname: Meutia
authors.affiliation: Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung, Indonesia.
authors.forename: Fernita
authors.initials: F
authors.lastname: Puspasari
authors.affiliation: Bioscience and Biotechnology Research Center, Institut Teknologi Bandung, Bandung, Indonesia.|Biochemistry Laboratory, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, West Java, Indonesia.
authors.forename: Khomaini
authors.initials: K
authors.lastname: Hasan
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism*; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins
keywords: Alkyl hydroperoxide; Alkyl hydroperoxide reductase; Oxidative stress