title: Sequence and structure analysis of a mirror tRNA located upstream of the cytochrome oxidase I mRNA in mouse mitochondria.
abstract: RNA fragments corresponding to the mirror tRNA that is located upstream of the cytochrome oxidase I (COXI) gene in the mouse mitochondrial genome were found in the sequences obtained from the mouse brain by the next generation sequencing. RNA fragments corresponding to the 5' terminal of COXI mRNA were also found and it was suggested that the precursor of the COXI mRNA is processed at three residues upstream of the first AUG codon. The mirror tRNA fragment has poly(A) in its 3' terminal and variable 5' terminal, suggesting that this RNA is produced during the 5' processing of COXI mRNA. Secondary structure prediction and NMR analysis indicated that the mirror tRNA is folded into a tRNA-like secondary structure, suggesting that the tRNA-like conformation of the 5' adjacent sequence of COXI mRNA is involved in the COXI mRNA maturation in the mouse mitochondria.
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Tsudanuna 2-17-1, Narashino, Chiba 275-0016, Japan;
authors.forename: Saya
authors.initials: S
authors.lastname: Okui
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Aomori 036-8560, Japan;
authors.forename: Chisato
authors.initials: C
authors.lastname: Ushida
authors.affiliation: Department of Environmental Medicine, Kochi Medical School, Kochi University,Kochi 783-8505, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kiyosawa
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, Tsudanuna 2-17-1, Narashino, Chiba 275-0016, Japan; gkawai@sea.it-chiba.ac.jp.
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D018387:Codon, Initiator / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D054629:Genome, Mitochondrial; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D011061:Poly A / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000737:chemistry*; D012343:RNA, Transfer / Q000235:genetics*; D017423:Sequence Analysis, RNA
chemical_list: D018387:Codon, Initiator; D012333:RNA, Messenger; D058727:RNA, Small Untranslated; D011061:Poly A; D012343:RNA, Transfer; D003576:Electron Transport Complex IV
keywords: NMR; mitochondria; sequencer; structure analysis; tRNA