title: Transcriptome analysis of genes involved in defence response in Polyporus umbellatus with Armillaria mellea infection.
abstract: Polyporus umbellatus, a species symbiotic with Armillaria mellea and it also exhibits substantial defence response to Armillaria mellea infection. There are no genomics resources databases for understanding the molecular mechanism underlying the infection stress of P. umbellatus. Therefore, we performed a large-scale transcriptome sequencing of this fungus with A. mellea infection using Illumina sequencing technology. The assembly of the clean reads resulted in 120,576 transcripts, including 38,444 unigenes. Additionally, we performed a gene expression profiling analysis upon infection treatment. The results indicated significant differences in the gene expression profiles between the control and the infection group. In total, 10933 genes were identified between the two groups. Based on the differentially expressed genes, a Gene Ontology annotation analysis showed many defence-relevant categories. Meanwhile, the Kyoto Encyclopedia of Genes and Genomes pathway analysis uncovered some important pathways. Furthermore, the expression patterns of 13 putative genes that are involved in defence response resulting from quantitative real-time PCR were consistent with their transcript abundance changes as identified by RNA-seq. The sequenced genes covered a considerable proportion of the P. umbellatus transcriptome, and the expression results may be useful to strengthen the knowledge on the defence response of this fungus defend against Armillaria mellea invasion.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193, P. R. China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Liu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193, P. R. China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Xing
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193, P. R. China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &Peking Union Medical College, Beijing 100193, P. R. China.
authors.forename: Shun-Xing
authors.initials: SX
authors.lastname: Guo
mesh_terms: D055392:Armillaria / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology; D055492:Polyporus / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D018832:Molecular Chaperones; D012331:RNA, Fungal