title: DNA Origami Nanoantennas with over 5000-fold Fluorescence Enhancement and Single-Molecule Detection at 25 μM.
abstract: Optical nanoantennas are known to focus freely propagating light and reversely to mediate the emission of a light source located at the nanoantenna hotspot. These effects were previously exploited for fluorescence enhancement and single-molecule detection at elevated concentrations. We present a new generation of self-assembled DNA origami based optical nanoantennas with improved robustness, reduced interparticle distance, and optimized quantum-yield improvement to achieve more than 5000-fold fluorescence enhancement and single-molecule detection at 25 μM background fluorophore concentration. Besides outperforming lithographic optical antennas, DNA origami nanoantennas are additionally capable of incorporating single emitters or biomolecular assays at the antenna hotspot.
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Puchkova
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Vietz
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Enrico
authors.initials: E
authors.lastname: Pibiri
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Wünsch
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: María
authors.initials: M
authors.lastname: Sanz Paz
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Guillermo P
authors.initials: GP
authors.lastname: Acuna
authors.affiliation: Institute for Physical & Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology , 38106 Braunschweig, Germany.
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld
mesh_terms: D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles; D018613:Microscopy, Confocal; D049329:Nanostructures
chemical_list: D006046:Gold; D004247:DNA
keywords: DNA origami; fluorescence enhancement; gold nanoparticles; plasmonics; single-molecule detection