title: Emergence and Characterization of Unusual DS-1-Like G1P[8] Rotavirus Strains in Children with Diarrhea in Thailand.
abstract: The emergence and rapid spread of unusual DS-1-like G1P[8] rotaviruses in Japan have been recently reported. During rotavirus surveillance in Thailand, three DS-1-like G1P[8] strains (RVA/Human-wt/THA/PCB-180/2013/G1P[8], RVA/Human-wt/THA/SKT-109/2013/G1P[8], and RVA/Human-wt/THA/SSKT-41/2013/G1P[8]) were identified in stool specimens from hospitalized children with severe diarrhea. In this study, we sequenced and characterized the complete genomes of strains PCB-180, SKT-109, and SSKT-41. On whole genomic analysis, all three strains exhibited a unique genotype constellation including both genogroup 1 and 2 genes: G1-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. This novel genotype constellation is shared with Japanese DS-1-like G1P[8] strains. Phylogenetic analysis revealed that the G/P genes of strains PCB-180, SKT-109, and SSKT-41 appeared to have originated from human Wa-like G1P[8] strains. On the other hand, the non-G/P genes of the three strains were assumed to have originated from human DS-1-like strains. Thus, strains PCB-180, SKT-109, and SSKT-41 appeared to be derived through reassortment event(s) between Wa-like G1P[8] and DS-1-like human rotaviruses. Furthermore, strains PCB-180, SKT-109, and SSKT-41 were found to have the 11-segment genome almost indistinguishable from one another in their nucleotide sequences and phylogenetic lineages, indicating the derivation of the three strains from a common origin. Moreover, all the 11 genes of the three strains were closely related to those of Japanese DS-1-like G1P[8] strains. Therefore, DS-1-like G1P[8] strains that have emerged in Thailand and Japan were assumed to have originated from a recent common ancestor. To our knowledge, this is the first report on whole genome-based characterization of DS-1-like G1P[8] strains that have emerged in an area other than Japan. Our observations will provide important insights into the evolutionary dynamics of emerging DS-1-like G1P[8] rotaviruses.
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komoto
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Ratana
authors.initials: R
authors.lastname: Tacharoenmuang
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Ratigorn
authors.initials: R
authors.lastname: Guntapong
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Ide
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Haga
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Takema
authors.initials: T
authors.lastname: Kato
authors.affiliation: Genome and Transcriptome Analysis Center, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Ouchi
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.|Genome and Transcriptome Analysis Center, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
authors.affiliation: Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Tsuji
authors.affiliation: Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
authors.forename: Somchai
authors.initials: S
authors.lastname: Sangkitporn
authors.affiliation: Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D019143:Evolution, Molecular; D014644:Genetic Variation / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics; D023281:Genomics / Q000379:methods; D005838:Genotype; D006801:Humans; D007223:Infant; D007564:Japan; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000379:methods; D013785:Thailand
chemical_list: D012367:RNA, Viral