title: Global changes of phospholipids identified by MALDI imaging mass spectrometry in a mouse model of Alzheimer's disease.
abstract: Alzheimer's disease (AD) is the most common form of dementia; however, at the present time there is no disease-modifying drug for AD. There is increasing evidence supporting the role of lipid changes in the process of normal cognitive aging and in the etiology of age-related neurodegenerative diseases. AD is characterized by the presence of intraneuronal protein clusters and extracellular aggregates of β-amyloid (Aβ). Disrupted Aβ kinetics may activate intracellular signaling pathways, including tau hyperphosphorylation and proinflammatory pathways. We analyzed and visualized the lipid profiles of mouse brains using MALDI-TOF MS. Direct tissue analysis by MALDI-TOF imaging MS (IMS) can determine the relative abundance and spatial distribution of specific lipids in different tissues. We used 5XFAD mice that almost exclusively generate and rapidly accumulate massive cerebral levels of Aβ-42 (1). Our data showed changes in lipid distribution in the mouse frontal cortex, hippocampus, and subiculum, where Aβ plaques are first generated in AD. Our results suggest that MALDI-IMS is a powerful tool for analyzing the distribution of various phospholipids and that this application might provide novel insight into the prediction of disease.
authors.affiliation: Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Kang
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Dong Kyu
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Baik
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Selina Rahman
authors.initials: SR
authors.lastname: Shanta
authors.affiliation: Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Sciences, Kyung Hee University, Yongin, Republic of Korea.
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Inhee
authors.initials: I
authors.lastname: Mook-Jung
authors.affiliation: Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Sciences, Kyung Hee University, Yongin, Republic of Korea kimkp@khu.ac.kr.
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D053764:Presenilin-1 / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C545487:APP protein, human; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; C508853:PSEN1 protein, human; D010743:Phospholipids; D053764:Presenilin-1
keywords: MS/MS (LIFT); matrix-assisted laser desorption/ionization; principal component analysis