title: Double Copies of bla(KPC-3)::Tn4401a on an IncX3 Plasmid in Klebsiella pneumoniae Successful Clone ST512 from Italy.
abstract: A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla(KPC) genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla(KPC-3) gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla(KPC-3)::Tn4401a caused by intramolecular transposition events were detected in the plasmid.
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fortini
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Villa
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Feudi
authors.affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
authors.forename: João
authors.initials: J
authors.lastname: Pires
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care G. D'Alessandro, University of Palermo, Palermo, Italy.
authors.forename: Celestino
authors.initials: C
authors.lastname: Bonura
authors.affiliation: Department of Sciences for Health Promotion and Mother-Child Care G. D'Alessandro, University of Palermo, Palermo, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Mammina
authors.affiliation: Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
authors.forename: Andrea
authors.identifier: http://orcid.org/0000-0003-3186-5421
authors.initials: A
authors.lastname: Endimiani
authors.affiliation: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy Institute for Infectious Diseases, University of Bern, Bern, Switzerland alecara@iss.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Carattoli
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015780:Carbapenems / Q000494:pharmacology; D002999:Clone Cells; D004251:DNA Transposable Elements; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D007558:Italy; D007710:Klebsiella Infections / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C063912:carbapenemase