title: Influence of doxazosin on biosynthesis of S100A6 and atrial natriuretic factor peptides in the heart of spontaneously hypertensive rats.
abstract: Hypertension frequently results in severe complications in cardiovascular system and histopathological changes in the heart. To better understand the cellular processes and signaling pathways responsible for the proper functioning of the heart, we decided to check whether doxazosin affects the density of structures containing S100A6 and atrial natriuretic factor in the heart of spontaneously hypertensive rats. The aim of this study is to find differences in the density of the structures containing S100A6 and atrial natriuretic factor in the heart of spontaneously hypertensive rats treated with doxazosin compared to untreated animals. Fragments of heart were collected from five spontaneously hypertensive rats and five spontaneously hypertensive rats receiving doxazosin for six weeks (dose 0.1 mg per 1 kg of body weight). On the paraffin sections S100A6 and atrial natriuretic factor peptides were localized in the heart using immunohistochemistry. Positive immunohistochemical reaction for S100A6 was observed in atrial and ventricular cardiomyocytes and in the coronary vasculature. In the heart of hypertensive rats treated with doxazosin the S100A6 immunoreactivity was significantly lower compared to untreated animals. Immunodetection of atrial natriuretic factor in the heart of rats confirmed presence of peptide in atrial myocardium. Delicate atrial natriuretic factor-immunoreactivity was observed also in few ventricular cardiomyocytes. The atrial natriuretic factor-immunosignal was significantly weaker in hearts of hypertensive rats receiving doxazosin compared to spontaneously hypertensive rats untreated. Since we found that doxazosin reduces the levels of S100A6 and atrial natriuretic factor peptides in the heart of spontaneously hypertensive rats, it can be assumed that cardiovascular disorders that occur in hypertension may be associated with disturbances of cellular processes and signaling pathways.
authors.affiliation: Department of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C, 15-222 Białystok, Poland kasacka@umb.edu.pl.
authors.forename: Irena
authors.initials: I
authors.lastname: Kasacka
authors.affiliation: Department of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C, 15-222 Białystok, Poland.
authors.forename: Żaneta
authors.initials: Ż
authors.lastname: Piotrowska
authors.affiliation: Nencki Institute of Experimental Biology, Laboratory of Calcium Binding Proteins, 02-093 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Filipek
authors.affiliation: Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Majewski
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D018797:Cell Cycle Proteins / Q000032:analysis*; D017292:Doxazosin / Q000008:administration & dosage*; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000737:chemistry; D011918:Rats, Inbred SHR; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000032:analysis*
chemical_list: D000959:Antihypertensive Agents; D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; D009320:Atrial Natriuretic Factor; D017292:Doxazosin
keywords: ANF; S100A6; doxazosin; heart; rat; spontaneously hypertension