title: Production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes.
abstract: Production of polyhydroxyalkanoate (PHA) co-polymers by Bacillus spp. was studied by feeding defined volatile fatty acids (VFAs) obtained through controlled hydrolysis of various wastes. Eleven mixed hydrolytic cultures (MHCs) each containing 6 strains could generate VFA from slurries of (2% total solids): pea-shells (PS), potato peels (PP), apple pomace (AP) and onion peels (OP). PS hydrolysates (obtained with MHC2 and MHC5) inoculated with Bacillus cereus EGU43 and Bacillus thuringiensis EGU45 produced co-polymers of PHA at the rate of 15-60mg/L with a 3HV content of 1%w/w. An enhancement in PHA yield of 3.66-fold, i.e. 205-550mg/L with 3HV content up to 7.5%(w/w) was observed upon addition of OP hydrolysate and 1% glucose (w/v) to PS hydrolysates. This is the first demonstration, where PHA co-polymer composition, under non-axenic conditions, could be controlled by customizing VFA profile of the hydrolysate by the addition of different biowastes.
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Department of Biotechnology, Savitribai Phule Pune University, Pune 411007, India. Electronic address: prasun.mcr@gmail.com.
authors.forename: Prasun
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Academy of Scientific & Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: Subhasree
authors.initials: S
authors.lastname: Ray
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India; Academy of Scientific & Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi 110001, India.
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
mesh_terms: D001407:Bacillus; D001412:Bacillus subtilis; D001413:Bacillus thuringiensis; D001709:Biotechnology / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry; D005232:Fatty Acids, Volatile / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D027845:Malus; D019697:Onions; D018532:Pisum sativum; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011513:Proteus mirabilis; D012037:Refuse Disposal / Q000379:methods*; D011198:Solanum tuberosum
chemical_list: D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D005947:Glucose
keywords: Bacillus; Biowaste; Co-polymer; Defined mixed culture; Volatile fatty acids