title: Benzothiazole-Pyimidine-Based BF2 Complex for Selective Detection of Cysteine.
abstract: Due to the similar structure and reactivity of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), the simultaneous discrimination of Cys over Hcy and GSH by a single fluorescent sensor is still a great challenge. In this work, a benzothiazole-pyimidine-based boron difluoride complex (BPB) was developed as a new fluorescent sensor for Cys. The sensor exhibits a highly selective "turn-on" response to cysteine over Hcy, GSH and other amino acids in aqueous solution at physiological pH. The observed pseudo-first-order rate constant for the reaction of BPB with Cys was calculated to be about 0.062 min(-1) . The detection limit of this sensor for Cys was determined to be 332 nm, and bioimaging of exogenous Cys by this sensor was successfully applied in living cells, thus indicating that this sensor holds great potential for biological applications.
authors.affiliation: School of Chemistry and Chemical Engineering & School of Material Science and Engineering, Liaocheng University, No.1 Hunan Road, Liaocheng, 252000, P. R. China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of chemistry, Nanjing Xiaozhuang College, 3601 Hongjing Road, Nanjing, 211171, P. R. China.
authors.forename: Changli
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Chemistry and Chemical Engineering & School of Material Science and Engineering, Liaocheng University, No.1 Hunan Road, Liaocheng, 252000, P. R. China. wangxiaoqing@lcu.edu.cn.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering & School of Material Science and Engineering, Liaocheng University, No.1 Hunan Road, Liaocheng, 252000, P. R. China. gongshw@lcu.edu.cn.
authors.forename: Shuwen
authors.initials: S
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Hankou Road No. 22, Nanjing, 210093, P. R. China.
authors.forename: Weijiang
authors.initials: W
authors.lastname: He
authors.affiliation: School of Chemistry and Chemical Engineering & School of Material Science and Engineering, Liaocheng University, No.1 Hunan Road, Liaocheng, 252000, P. R. China. iamzpliu@njtech.edu.cn.|Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (Nanjing Tech), 30 South Puzhu Road, Nanjing, 211816, P. R. China. iamzpliu@njtech.edu.cn.
authors.forename: Zhipeng
authors.identifier: http://orcid.org/0000-0002-5458-9362
authors.initials: Z
authors.lastname: Liu
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry*; D015374:Biosensing Techniques; D003545:Cysteine / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D006367:HeLa Cells; D006710:Homocysteine / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D015394:Molecular Structure; D011743:Pyrimidines / Q000737:chemistry*
chemical_list: D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011743:Pyrimidines; D006710:Homocysteine; C005465:benzothiazole; D005978:Glutathione; D003545:Cysteine
keywords: BODIPY; cysteine; fluorescent sensing; fluorescent sensors; selectivity