title: Electrochemical immunosensor based on Pd-Au nanoparticles supported on functionalized PDDA-MWCNT nanocomposites for aflatoxin B1 detection.
abstract: This paper reports a label-free electrochemical immunosensor for the determination of aflatoxin B1 (AFB1), which is based on a gold electrode modified by a biocompatible film of carbon nanotubes/poly(diallyldimethylammoniumchloride)/Pd-Au nanoparticles (CNTs/PDDA/Pd-Au). The nanocomposite was characterized by transmission electron microscopy and the electrochemical behavior of modified electrodes was investigated by cyclic voltammetry. The CNTs/PDDA/Pd-Au nanocomposites film showed good electron transfer ability, which ensured high sensitivity to detect AFB1 in a range from 0.05 to 25 ng mL(-1) with a detection limit of 0.03 ng mL(-1) obtained at 3σ (where σ is the standard deviation of the blank solution, n = 10). The proposed immunosensor provides a simple tool for AFB1 detection. This strategy can be extended to any other antigen detection by using the corresponding antibodies.
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China; State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China. Electronic address: sgyrab@163.com.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.affiliation: Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Shen
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of China.
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000276:immunology; D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D012275:Oryza / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D010165:Palladium; D006046:Gold; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Carbon nanotubes; Immunosensors; Pd–Au nanoparticles; Poly(diallyldimethylammoniumchloride)