title: Comparative evaluation of effect of different irrigation solutions against Enterococcus faecalis: A polymerase chain reaction-based study.
abstract: Enterococcus faecalis is the most isolated or detected species from oral infections including marginal periodontitis, infected root canals, periradicular abscesses and also detected in cases of failed endodontic therapy. To prevent endodontic treatment failure irrigation is mandatory for the effective removal of smear layer, pulp tissue, and microorganisms. Cultivation and other traditional identification methods have been demonstrated to have several limitations when it comes to microbiological identification. Polymerase chain reaction was selected because it has an added advantage over traditional microbiological methods.
  
  
  METHODS
  Twenty single rooted premolars were taken were taken stored in 0.1% thymol solution at 4°C decoronated to obtain 12 mm length, teeth were autoclaved at 121°C, canals were instrumented up to 35k file (International Organization for Standardization). The samples were randomly divided into three groups Group I - 5.25% sodium hypochlorite (NaOCL) and 17% ethylenediaminetetraacetic acid (EDTA), Group - II 5.25% NaOCL and 2% chlorhexidine (CHX), Group III - 5.25% NaOCL and 17% EDTA and 2% CHX.
  
  
  RESULTS
  The results showed that Group III which is 5% NaOCl followed by 17% EDTA and followed by 2% CHX showed maximum antimicrobial activity in all the three different time intervals.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Rajas Dental College, Tirunelveli, Tamil Nadu, India.
authors.forename: R Gnana
authors.initials: RG
authors.lastname: Seelan
authors.affiliation: Department of Conservative Dentistry and Endodontics, Rajas Dental College, Tirunelveli, Tamil Nadu, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Conservative Dentistry and Endodontics, Rajas Dental College, Tirunelveli, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Jonathan
authors.affiliation: Department of Conservative Dentistry and Endodontics, Rajas Dental College, Tirunelveli, Tamil Nadu, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Maheswari
authors.affiliation: Department of Periodontics, Rajas Dental College, Tirunelveli, Tamil Nadu, India.
authors.forename: Jacob
authors.initials: J
authors.lastname: Raja
authors.affiliation: Department of Paedodontics and Preventive Dentistry, Sri Mookambika Institute Of Dental Sciences, Kulasekharam, Kanyakumari, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Chelliah
keywords: Enterococcus faecalis; irrigants; polymerase chain reaction