title: Biotic and abiotic roles of leachate recirculation in batch mode solid-state anaerobic digestion of cattle manure.
abstract: Solid state anaerobic digestion, with leachate recirculation, is suitable for exploiting manure with a high solid content. The biotic and abiotic effects of the leachates were studied in lab-scale leach bed reactors (LBRs). LBRs were fed with cow manure and four leachates either biologically active or inert. The biotic impact of leachate was assessed by monitoring the microbial communities in the manure and in the leachates. LBRs with biologically active leachates, regardless to their origin, produced equivalent methane volumes (114.52±19.05 and 99.79±6.4NL/kgVS) while LBRs with inert leachates produced half less methane (60.22±5.71 and 58.87±13.2NL/kgVS) attesting to the biotic role of leachate. Moreover, its beneficial abiotic role is mainly due to its initial nutrient content, pH, and buffering capacity. The microbial community in the manure was strongly involved in methane production, and no transfer of microorganisms from the liquid phase was found (p<0.05).
authors.affiliation: Irstea, UR GERE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, F-35044 Rennes, France.
authors.forename: Axelle
authors.initials: A
authors.lastname: Degueurce
authors.affiliation: Irstea, UR GERE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, F-35044 Rennes, France.
authors.forename: Nair
authors.initials: N
authors.lastname: Tomas
authors.affiliation: Irstea, UR GERE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, F-35044 Rennes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Le Roux
authors.affiliation: Irstea, UR GERE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, F-35044 Rennes, France.
authors.forename: José
authors.initials: J
authors.lastname: Martinez
authors.affiliation: Irstea, UR GERE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France; Université Européenne de Bretagne, F-35044 Rennes, France. Electronic address: pascal.peu@irstea.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Peu
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001105:Archaea; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002021:Buffers; D002417:Cattle; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008372:Manure; D008697:Methane / Q000737:chemistry*; D018807:Polymorphism, Single-Stranded Conformational; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D012037:Refuse Disposal / Q000379:methods*; D013696:Temperature
chemical_list: D056804:Biofuels; D002021:Buffers; D008372:Manure; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008697:Methane
keywords: Leach bed reactor; Leachate; Manure; Methane production rate; Single strand conformation polymorphism