title: High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil.
abstract: OBJECTIVE
  The goals of the present study were to identify, to analyse the phylogenetic relations and to evaluate the genetic variability in Diaporthe endophytic isolates from common bean.
  
  
  RESULTS
  Diaporthe sp., D. infecunda and D. phaseolorum strains were identified using multilocus phylogeny (rDNA ITS region; EF1-α, β-tubulin, and calmodulin genes). IRAP (Inter-Retrotransposon Amplified Polymorphism) and REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) molecular markers reveal the existence of high genetic variability, especially among D. infecunda isolates.
  
  
  CONCLUSIONS
  It was concluded that the multilocus phylogenetic approach was more effective than individual analysis of ITS sequences, in identifying the isolates to species level, and that IRAP and REMAP markers can be used for studying the genetic variability in the genus Diaporthe particularly at the intraspecific level.
  
  
  CONCLUSIONS
  The combined use of molecular tools such as multilocus phylogenetic approach and molecular markers, as performed in this study, is the best way to distinguish endophytic strains of Diaporthe isolated from common bean (Phaseolus vulgaris L.).
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.|Instituto de Ciências e Tecnologia das Águas, Universidade Federal do Oeste do Pará, Santarém, Brazil.
authors.forename: T T
authors.initials: TT
authors.lastname: Dos Santos
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: de Souza Leite
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: C B
authors.initials: CB
authors.lastname: de Queiroz
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: de Araújo
authors.affiliation: Department of Phytopathology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: O L
authors.initials: OL
authors.lastname: Pereira
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Brazil.
authors.forename: M V
authors.initials: MV
authors.lastname: de Queiroz
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001938:Brazil; D060026:Endophytes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000382:microbiology*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D014404:Tubulin / Q000235:genetics
chemical_list: D014404:Tubulin
keywords: DNA fingerprinting; Diaporthe; IRAP; MLST; REMAP; endophytic fungus; genetic variability