title: Genotype-dependent lifespan effects in peptone deprived Caenorhabditis elegans.
abstract: Dietary restriction appears to act as a general non-genetic mechanism that can robustly prolong lifespan. There have however been reports in many systems of cases where restricted food intake either shortens, or does not affect, lifespan. Here we analyze lifespan and the effect of food restriction via deprived peptone levels on lifespan in wild isolates and introgression lines (ILs) of the nematode Caenorhabditis elegans. These analyses identify genetic variation in lifespan, in the effect of this variation in diet on lifespan and also in the likelihood of maternal, matricidal, hatching. Importantly, in the wild isolates and the ILs, we identify genotypes in which peptone deprivation mediated dietary restriction reduces lifespan. We also identify, in recombinant inbred lines, a locus that affects maternal hatching, a phenotype closely linked to dietary restriction in C. elegans. These results indicate that peptone deprivation mediated dietary restriction affects lifespan in C. elegans in a genotype-dependent manner, reducing lifespan in some genotypes. This may operate by a mechanism similar to dietary restriction.
authors.affiliation: Biomolecular Research Group, Canterbury Christ Church University, Canterbury, CT1 1QU, UK.
authors.forename: Jana J
authors.initials: JJ
authors.lastname: Stastna
authors.affiliation: Laboratory of Nematology, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: L Basten
authors.initials: LB
authors.lastname: Snoek
authors.affiliation: Laboratory of Nematology, Wageningen University, 6708 PB Wageningen, The Netherlands.
authors.forename: Jan E
authors.initials: JE
authors.lastname: Kammenga
authors.affiliation: Biomolecular Research Group, Canterbury Christ Church University, Canterbury, CT1 1QU, UK.
authors.forename: Simon C
authors.initials: SC
authors.lastname: Harvey
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D004032:Diet; D005838:Genotype; D008017:Life Expectancy; D010461:Peptones / Q000378:metabolism*
chemical_list: D010461:Peptones