title: Cell type- and brain region-resolved mouse brain proteome.
abstract: Brain transcriptome and connectome maps are being generated, but an equivalent effort on the proteome is currently lacking. We performed high-resolution mass spectrometry-based proteomics for in-depth analysis of the mouse brain and its major brain regions and cell types. Comparisons of the 12,934 identified proteins in oligodendrocytes, astrocytes, microglia and cortical neurons with deep sequencing data of the transcriptome indicated deep coverage of the proteome. Cell type-specific proteins defined as tenfold more abundant than average expression represented about a tenth of the proteome, with an overrepresentation of cell surface proteins. To demonstrate the utility of our resource, we focused on this class of proteins and identified Lsamp, an adhesion molecule of the IgLON family, as a negative regulator of myelination. Our findings provide a framework for a system-level understanding of cell-type diversity in the CNS and serves as a rich resource for analyses of brain development and function.
authors.affiliation: Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Kirti
authors.initials: K
authors.lastname: Sharma
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Neurology, University of Göttingen, Göttingen, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schmitt
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Neurology, University of Göttingen, Göttingen, Germany.
authors.forename: Caroline G
authors.initials: CG
authors.lastname: Bergner
authors.affiliation: Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Stefka
authors.initials: S
authors.lastname: Tyanova
authors.affiliation: Department of Psychiatry, Ludwig-Maximillian University, Munich, Germany.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Kannaiyan
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Neurology, University of Göttingen, Göttingen, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Manrique-Hoyos
authors.affiliation: Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Estonia.
authors.forename: Karina
authors.initials: K
authors.lastname: Kongi
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Neurology, University of Göttingen, Göttingen, Germany.
authors.forename: Ludovico
authors.initials: L
authors.lastname: Cantuti
authors.affiliation: Department of Neuropathology, University of Göttingen, Göttingen, Germany.
authors.forename: Uwe-Karsten
authors.initials: UK
authors.lastname: Hanisch
authors.affiliation: Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Estonia.
authors.forename: Mari-Anne
authors.initials: MA
authors.lastname: Philips
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Psychiatry, Ludwig-Maximillian University, Munich, Germany.
authors.forename: Moritz J
authors.initials: MJ
authors.lastname: Rossner
authors.affiliation: Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.affiliation: Max Planck Institute of Experimental Medicine, Göttingen, Germany.|Department of Neurology, University of Göttingen, Göttingen, Germany.
authors.forename: Mikael
authors.initials: M
authors.lastname: Simons
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology*; D020543:Proteome / Q000235:genetics*
chemical_list: D020543:Proteome