title: Development of surface immobilized 3-azidocoumarin-based fluorogenic probe via strain promoted click chemistry.
abstract: A new class of imaging probe, a fluorogenic version of 1, 3-dipolar cycloaddition of azides and alkynes has been developed. 3-azidocoumarin scaffolds were selectively immobilized on the DBCO modified bead surface via SPAAC and provide direct and strong fluorescence in fluorescence microscopy. This developed click-on beads could be applied to label various biomolecules, such as nucleic acids, proteins and other molecules. To this end, 5'(7-hydroxy 3-azido coumarin) labelled DNA primer also displayed strong fluorescence upon successful immobilization on the bead surface.
authors.affiliation: Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, Tamilnadu, India.
authors.forename: M Vijaya
authors.initials: MV
authors.lastname: Bharathi
authors.affiliation: Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, Tamilnadu, India.
authors.forename: Mohit
authors.initials: M
authors.lastname: Chhabra
authors.affiliation: Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore 632014, Tamilnadu, India. Electronic address: priyankar.paira@vit.ac.in.
authors.forename: Priyankar
authors.initials: P
authors.lastname: Paira
mesh_terms: D057930:Click Chemistry; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D003374:Coumarins; D017931:DNA Primers; D005456:Fluorescent Dyes; D011137:Polystyrenes; D004247:DNA; C030123:coumarin
keywords: 3-Azido coumarin; Click chemistry; Fluorogenic probe; Polystyrene beads; SPAAC