title: Preparation and characterization of carboxymethylated carrageenan modified with collagen peptides.
abstract: The preparation of carboxymethyl κ-carrageenan collagen peptide (CMKC-COP) was via an imide-bond forming reaction between carboxyl groups in carboxymethyl κ-carrageenan (CMKC) and amino groups in collagen peptide in the presence of 1-ethyl-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy sulfosuccinimide (NHS). CMKC-COP products were verified with infrared spectroscopy (FT-IR). The results of degree of substitution (DS) of CMKC-COP was presented, which are depended on reaction time, molar ratio of collagen peptide to carboxymethyl κ-carrageenan and reaction temperature. The optimal reaction conditions were studied by means of single factor experiment. Also MTT assay was applied to evaluate the effects of CMKC-COP on proliferation of chick embryo fibroblasts. The animal experiment results indicated that the wound covered with CMKC-COP were completely filled with new epithelium within 2 weeks without any significant adverse side reactions. Therefore, the CMKC-COP showed the potentiality to repair skin in cosmetic, biomedical and pharmaceutical fields.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lihongfan2000@hotmail.com.
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tong
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Tang
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wu
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Peng
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Yi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002351:Carrageenan / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003094:Collagen / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D016166:Free Radical Scavengers; D010455:Peptides; D002351:Carrageenan; D003094:Collagen
keywords: Carboxymethyl κ-carrageenan collagen peptide; Cell viability; Wound healing