title: [Experimental modeling of nucleoprotein disposal disorders in systemic lupus erythematosus].
abstract: The objective of this research was to adapt the experimental model simulating the nucleoprotein disposal disorders in systemic lupus erythematosus (SLE) for further study of its extracorporeal correction, as well as to assess validity of the model by short-term experiment. Twenty to female Wistar rats were intraperitoneally injected with the chromatin-containing extract from bovine liver followed by intravenous administration of anti-DNA antibodies derived from SLE patients. After these procedures plasma concentrations of anti-dsDNA, circulating immune complexes and DNA became sharply increased, together with distinct elevation of leukocytes. On the contrary, changes in erythrocytes, platelets, total protein concentration, creatinine, asparagine and alanine aminotransferase activities, as well as blood coagulation time were changed insignificantly. Using direct immunofluorescence of cryosections, we detected human IgG deposition in rat kidneys treated in accordance with the simulation protocol. Thus, our model reproduces essential DNA disposal disorders in SLE without any animal death or the life-threatening changes in examined markers during short-term experiment.
authors.affiliation: Research Institute for Clinical and Experimental Rheumatology RAMS, Volgograd, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Trofimenko
authors.affiliation: Research Institute for Clinical and Experimental Rheumatology RAMS, Volgograd, Russia.
authors.forename: I P
authors.initials: IP
authors.lastname: Gontar
authors.affiliation: Volgograd State Medical University, Volgograd, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Paramonova
authors.affiliation: Research Institute for Clinical and Experimental Rheumatology RAMS.
authors.forename: E S
authors.initials: ES
authors.lastname: Simakova
authors.affiliation: Research Institute for Clinical and Experimental Rheumatology RAMS.
authors.forename: I A
authors.initials: IA
authors.lastname: Zborovskaya
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology / Q000473:pathology; D002417:Cattle; D045424:Complex Mixtures / Q000008:administration & dosage / Q000276:immunology; D003404:Creatinine / Q000097:blood; D004247:DNA / Q000097:blood* / Q000276:immunology; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007668:Kidney / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D008099:Liver / Q000737:chemistry / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D009698:Nucleoproteins / Q000097:blood* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D000637:Transaminases / Q000097:blood
chemical_list: D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D045424:Complex Mixtures; D007074:Immunoglobulin G; D009698:Nucleoproteins; D004247:DNA; D003404:Creatinine; D000637:Transaminases; D000410:Alanine Transaminase; C022636:serine-glyoxylate aminotransferase
keywords: DNase I; anti-DNA antibodies; biosimulation; chromatin; systemic lupus erythematosus