title: Electroblotting from Polyacrylamide Gels.
abstract: Transferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N-terminal and internal sequencing using Edman chemistry. This unit contains procedures for electroblotting proteins from polyacrylamide gels onto a variety of membranes, including polyvinylidene difluoride (PVDF) and nitrocellulose. In addition to the commonly used tank or wet transfer system, protocols are provided for electroblotting using semidry and dry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents for specialized applications.
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania.
authors.forename: Aaron
authors.initials: A
authors.lastname: Goldman
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania.
authors.forename: Jeanine A
authors.initials: JA
authors.lastname: Ursitti
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania.
authors.forename: Jacek
authors.initials: J
authors.lastname: Mozdzanowski
authors.affiliation: The Wistar Institute, Philadelphia, Pennsylvania.
authors.forename: David W
authors.initials: DW
authors.lastname: Speicher
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D008567:Membranes, Artificial; D011506:Proteins
keywords: SDS-PAGE gel transfer; electroblotting; electrotransfer; polyacrylamide gel transfer; protein transfer; western blotting