title: Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.
abstract: A novel competitive host-guest strategy regulated by protein biogate was developed for sensitive and selective analysis of prion protein. The methylene blue (MB)-tagged prion aptamer (MB-Apt) was introduced to the multiwalled carbon nanotubes-β-cyclodextrins (MWCNTs-β-CD) composites-modified glassy carbon (GC) electrode through the host-guest interaction between β-CD and MB. In the absence of prion, MB-Apt could be displaced by ferrocenecarboxylic acid (FCA) due to its stronger binding affinity to β-CD, resulting in a large oxidation peak of FCA. However, in the presence of prion, the specific prion-aptamer interaction drove the formation of protein biogate to seal the cavity of β-CD, which hindered the guest displacement of MB by FCA and resulted in the oxidation peak current of MB (IMB) increased and that of FCA (IFCA) decreased. The developed aptasensor showed good response towards the target (prion protein) with a low detection limit of 160 fM. By changing the specific aptamers, this strategy could be easily extended to detect other proteins, showing promising potential for extensive applications in bioanalysis.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Jiawan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Erhu
authors.initials: E
authors.lastname: Xiong
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D057230:Limit of Detection; D000075163:Metallocenes; D008751:Methylene Blue / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction; D011328:Prions / Q000032:analysis* / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D052158:Aptamers, Peptide; D005296:Ferrous Compounds; D000075163:Metallocenes; D037742:Nanotubes, Carbon; D011328:Prions; D047392:beta-Cyclodextrins; C032949:ferrocenecarboxylic acid; D008751:Methylene Blue