title: Assessment of Retrograde Coronary Venous Infusion of Mesenchymal Stem Cells Combined with Basic Fibroblast Growth Factor in Canine Myocardial Infarction Using Strain Values Derived from Speckle-Tracking Echocardiography.
abstract: Speckle-tracking echocardiography was used to assess retrograde coronary venous infusion of mesenchymal stem cells (MSCs) combined with basic fibroblast growth factor (bFGF) in a canine model of acute myocardial infarction (AMI). AMI was induced by ligation of the left anterior descending coronary artery. Coronary venous retroperfusion was performed at 1 wk after AMI. Twenty-eight animals were randomized into four groups: saline, bFGF+saline, saline+MSCs and bFGF+MSCs. Echocardiography was performed before AMI, at 7 d post-AMI and 40 d after retroperfusion. Apoptotic cardiomyocytes in the border zone of the ischemic region were evaluated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling. Vascular endothelial growth factor and factor VIII concentrations were measured by western blotting. The left ventricular end-systolic volume increased significantly, whereas the left ventricular ejection fraction and global and segmental strain values decreased significantly after AMI. After retroperfusion, the strain values of the infarct zone, but not conventional echocardiographic parameters, were significantly different between control and bFGF+MSC groups. Cardiomyocyte apoptosis decreased, whereas vascular endothelial growth factor and factor VIII concentrations were higher in the bFGF+MSC, bFGF and MSC groups. Cardiomyocyte apoptosis was well correlated with the strain values. Although retrograde coronary venous infusion of bFGF and MSCs promoted neo-vascularization of the infarcted myocardium and inhibited apoptosis, there was only a slight strain improvement without a substantial increase in global cardiac functions.
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Sun
authors.affiliation: Emergency & Critical Care Center of Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhen
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Yang
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Yi-Jia
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Rong-Juan
authors.initials: RJ
authors.lastname: Li
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Zhi-An
authors.initials: ZA
authors.lastname: Li
authors.affiliation: Beijing Institute of Heart, Lung, and Blood Vessel Diseases and The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education, Beijing, China.
authors.forename: Lu-Ya
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Emergency & Critical Care Center of Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.affiliation: Ultrasound Department, Beijing AnZhen Hospital Affiliated with Capital Medical University, Beijing, China. Electronic address: Yangya99@hotmail.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000208:Acute Disease; D000818:Animals; D015153:Blotting, Western; D003331:Coronary Vessels / Q000000981:diagnostic imaging*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000628:therapy*; D012965:Sodium Chloride / Q000008:administration & dosage; D014463:Ultrasonography
chemical_list: D016222:Fibroblast Growth Factor 2; D012965:Sodium Chloride
keywords: Acute myocardial infarction; Canine; Mesenchymal stem cells; Retrograde coronary venous infusion; Speckle-tracking echocardiography; Strain analysis