title: Effects of Sleep Deprivation on Mice Bone Marrow and Spleen B Lymphopoiesis.
abstract: B lymphocytes are immune cells crucial for the maintenance and viability of the humoral response. Sleep is an essential event for the maintenance and integrity of all systems, including the immune system (IS). Thus, sleep deprivation (SD) causes problems in metabolism and homeostasis in many cell systems, including the IS. In this study, our goal was to determine changes in B lymphocytes from the bone marrow (BM) and spleen after SD. Three-month-old male Swiss mice were used. These mice were sleep deprived through the modified multiple platform method for different periods (24, 48, and 72 h), whereas another group was allowed to sleep for 24 h after 72 h of SD (rebound group) and a third group was allowed to sleep normally during the entire experiment. After this, the spleen and BM were collected, and cell analyses were performed. The numbers of B lymphocytes in the BM and spleen were reduced by SD. Additionally, reductions in the percentage of lymphocyte progenitors and their ability to form colonies were observed. Moreover, an increase in the death of B lymphocytes from the BM and spleen was associated with an increase in oxidative stress indicators, such as DCFH-DA, CAT, and mitochondrial SOD. Rebound was not able to reverse most of the alterations elicited by SD. The reduction in B lymphocytes and their progenitors by cell death, with a concomitant increase in oxidative stress, showed that SD promoted a failure in B lymphopoiesis.
authors.affiliation: Department of Psychobiology, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Lisandro
authors.initials: L
authors.lastname: Lungato
authors.affiliation: Department of Biophysics, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Nogueira-Pedro
authors.affiliation: Department de Biochemistry, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Carvalho Dias
authors.affiliation: Department de Biochemistry, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.|Centro Interdisciplinar de Investigação Bioquı́mica, Universidade de Mogi das Cruzes, Av. Dr Cândido Xavier de Almeida Souza, Mogi das Cruzes, SP, Brazil.
authors.forename: Edgar Julian
authors.initials: EJ
authors.lastname: Paredes-Gamero
authors.affiliation: Department of Psychobiology, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.affiliation: Department of Psychobiology, Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Vânia
authors.initials: V
authors.lastname: D'Almeida
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016923:Cell Death; D004195:Disease Models, Animal; D018655:Lymphocyte Count; D038041:Lymphopoiesis; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D054448:Precursor Cells, B-Lymphoid / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012892:Sleep Deprivation / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013154:Spleen / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D015415:Biomarkers