title: Establishment of human-embryonic-stem-cell line from mosaic trisomy 9 embryo.
abstract: OBJECTIVE
  Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders.
  
  
  METHODS
  In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of mosaic trisomy 9 embryos.
  
  
  RESULTS
  A karyotype analysis showed that the hESC line possessed a euploid (46 chromosomes). The undifferentiated hESCs exhibited long-term proliferation capacity and expressed typical markers of OCT4, TRA-1-60, and TRA-1-81. In vitro embryoid-body (EB) formation, differentiation, and in vivo teratoma production confirmed the pluripotency of the hESC line. The data represented here are the first detailed report on the characterization and differentiation of one Chinese hESC line generated from mosaic trisomy 9 embryos.
  
  
  CONCLUSIONS
  Our study showed that chromosomally aberrant embryos could generate a normal hESC line, which would be useful in investigating gene function and embryo development.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210038, China.
authors.forename: Boxian
authors.initials: B
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yugui
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jiazi
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Lingbo
authors.initials: L
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Liao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Song
authors.initials: S
authors.lastname: Ning
authors.affiliation: Department of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA.
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Jiang
authors.affiliation: Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Guoping
authors.initials: G
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. Electronic address: ljqin@njmu.edu.cn.
authors.forename: Lianju
authors.initials: L
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210038, China. Electronic address: jyliu_nj@126.com.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Liu
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D059785:Karyotype; D007621:Karyotyping; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D014314:Trisomy / Q000235:genetics*
keywords: euploid recovery; human embryonic stem cells; mosaic trisomy 9