title: Multiphoton imaging with a novel compact diode-pumped Ti:sapphire oscillator.
abstract: Multiphoton laser scanning microscopy commonly relies on bulky and expensive femtosecond lasers. We integrated a novel minimal-footprint Ti:sapphire oscillator, pumped by a frequency-doubled distributed Bragg reflector tapered diode laser, into a clinical multiphoton tomograph and evaluated its imaging capability using different biological samples, i.e. cell monolayers, corneal tissue, and human skin. With the novel laser, the realization of very compact Ti:sapphire-based systems for high-quality multiphoton imaging at a significantly size and weight compared to current systems will become possible.
authors.affiliation: JenLab GmbH, Jena, D-07745, Germany.|Department of Biophotonics and Laser Technology, Saarland University, Saarbrücken, D-66123, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: König
authors.affiliation: DTU Fotonik, Department of Photonics Engineering, Technical University of Denmark, Roskilde, DK- 4000, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Andersen
authors.affiliation: FemtoLasers, Vienna, A-1100, Austria.
authors.forename: Tuan
authors.initials: T
authors.lastname: Le
authors.affiliation: JenLab GmbH, Jena, D-07745, Germany.
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Breunig
mesh_terms: D002460:Cell Line; D003315:Cornea / Q000737:chemistry / Q000166:cytology; D006801:Humans; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D012867:Skin / Q000737:chemistry / Q000166:cytology
keywords: diode-pumped lasers; fluorescence microscopy; multiphoton imaging; ultrafast lasers