title: Occurrence and characterization of plum pox virus strain D isolates from European Russia and Crimea.
abstract: Numerous plum pox virus (PPV) strain D isolates have been found in geographically distant regions of European Russia and the Crimean peninsula on different stone fruit hosts. Phylogenetic analysis of their partial and complete genomes suggests multiple introductions of PPV-D into Russia. Distinct natural isolates from Prunus tomentosa were found to bear unique amino acid substitutions in the N-terminus of the coat protein (CP) that may contribute to the adaptation of PPV-D to this host. Serological analysis using the PPV-D-specific monoclonal antibody 4DG5 provided further evidence that mutations at positions 58 and 59 of the CP are crucial for antibody binding.
authors.affiliation: Lomonosov Moscow State University, Moscow, Russia. s-chirkov1@yandex.ru.
authors.forename: Sergei
authors.initials: S
authors.lastname: Chirkov
authors.affiliation: Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Peter
authors.initials: P
authors.lastname: Ivanov
authors.affiliation: Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Sheveleva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Kudryavtseva
authors.affiliation: All-Russian Center of Plant Quarantine, Moscow, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Prikhodko
authors.affiliation: Nikita Botanical Gardens, National Scientific Center, Yalta, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Mitrofanova
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D016000:Cluster Analysis; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017801:Plum Pox Virus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D011485:Protein Binding; D012367:RNA, Viral / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D012367:RNA, Viral
keywords: 454 Pyrosequencing; Phylogenetic analysis; Plum pox virus; Serological analysis; Strain D