title: Quinone-Modified Mn-Doped ZnS Quantum Dots for Room-Temperature Phosphorescence Sensing of Human Cancer Cells That Overexpress NQO1.
abstract: Early detection of cancer cells in a rapid and sensitive approach is one of the great challenges in modern clinical cancer care. This study has demonstrated the first example of a rapid, selective, and sensitive phosphorescence probe based on phosphorescence energy transfer (PET) for cancer-associated human
  quinone oxidoreductase isozyme 1 (NQO1). An efficient room-temperature phosphorescence NQO1 probe was constructed by using Mn-doped ZnS quantum dots (Mn:ZnS QDs) as donors and trimethylquinone propionic acids as acceptors. Phosphorescence quenching of Mn:ZnS QDs from the Mn:ZnS QDs to a covalently bonded quinone was achieved through PET. Phosphorescence of Mn:ZnS QDs was turned on by the rapid reduction-initiated removal of the quinone quencher by NQO1. This probe shows low cellular toxicity and can rapidly distinguish between NQO1-expressing and -nonexpressing cancer cell lines through phosphorescence imaging.
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Sung
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Srivardhan Reddy
authors.initials: SR
authors.lastname: Gayam
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Pei-Ying
authors.initials: PY
authors.lastname: Hsieh
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Hsin-Yun
authors.initials: HY
authors.lastname: Hsu
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Eric Wei-Guang
authors.initials: EW
authors.lastname: Diau
authors.affiliation: Department of Applied Chemistry and ‡Center for Interdisciplinary Science (CIS), National Chiao Tung University , Hsinchu 300, Taiwan.
authors.forename: Shu-Pao
authors.initials: SP
authors.lastname: Wu
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D055664:Electrochemical Techniques; D004735:Energy Transfer; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods*; D008345:Manganese / Q000737:chemistry*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D011809:Quinones / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013440:Sulfides / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D011809:Quinones; D013440:Sulfides; D017967:Zinc Compounds; D008345:Manganese; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; C031238:zinc sulfide
keywords: NQO1; cancer cells; phosphorescence energy transfer; quantum dots; room temperature phosphorescence