title: Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry.
abstract: Mass cytometry is flow cytometry based on single cell mass spectrometry with decreased crosstalk between channels and an ability to probe >40 parameters per cell, making it well suited for multiplexed assays. Peptide major histocompatibility (MHC) tetramer staining allows direct detection of antigen specific cells and is also amenable to multiplexing/combinatorial approaches. Here we describe methods for multiplexed pMHC-tetramer staining using mass cytometry.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, Singapore, 637551.
authors.forename: Mei Ling
authors.initials: ML
authors.lastname: Leong
authors.affiliation: Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR), 8A Biomedical Grove, Immunos #03-06, Singapore, Singapore, 138648. evan_newell@immunol.a-star.edu.sg.
authors.forename: Evan W
authors.initials: EW
authors.lastname: Newell
mesh_terms: D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007202:Indicators and Reagents; D008285:Major Histocompatibility Complex; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D059010:Single-Cell Analysis / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D007202:Indicators and Reagents; D010455:Peptides; D019809:Streptavidin
keywords: Antigen-specific T cell; Combinatorial tetramer staining; Mass cytometry; Peptide-MHC tetramer