title: Analysis of genetic variation in sorghum (Sorghum bicolor (L.) Moench) genotypes with various agronomical traits using SPAR methods.
abstract: Genetic variation among 45 genotypes of sorghum (Sorghum bicolor L.) representing seven subpopulations was assessed using three single primer amplification reaction (SPAR) methods viz., inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and directed amplification of minisatellite-region DNA (DAMD). Totally 15 ISSR, 8 RAPD and 7 DAMD primers generated 263 amplification products, accounting for 84.6% polymorphism across all the genotypes. The Mantel's test of correlation revealed the best correlation between ISSR and cumulative data with a correlation coefficient (r) of 0.84. Assessment of population diversity indicated that the maximum intra population genetic diversity was recorded among high FeZn lines (HFL) having maximum values of Nei's genetic diversity (h) (0.244), Shannon information index (I) (0.368) and the percentage of polymorphic loci (Pp) (72.65%) while the corresponding lowest values of 0.074, 0.109 and 17.95% respectively were observed among the members of MDT subpopulation. The mean coefficient of gene differentiation (GST) and the gene flow (Nm) between populations were observed to be 0.396 and 0.7680 respectively. The analysis of molecular variance (AMOVA) suggested that maximum genetic variation exists within populations (95%) than among populations (5%). Thus the information obtained from this study could be utilized in sorghum breeding programmes for the development of varieties with improved nutrition and agronomic values in future.
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India. Electronic address: pandu.pine@gmail.com.
authors.forename: Lakkakula
authors.initials: L
authors.lastname: Satish
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
authors.forename: Jayabalan
authors.initials: J
authors.lastname: Shilpha
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
authors.forename: Subramani
authors.initials: S
authors.lastname: Pandian
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
authors.forename: Arockiam Sagina
authors.initials: AS
authors.lastname: Rency
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
authors.forename: Periyasamy
authors.initials: P
authors.lastname: Rathinapriya
authors.affiliation: Division of Plant Biotechnology, Entomology Research Institute, Loyola College, Chennai 600 034, India.
authors.forename: Stanislaus Antony
authors.initials: SA
authors.lastname: Ceasar
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India.
authors.forename: Muthiah Joe Virgin
authors.initials: MJ
authors.lastname: Largia
authors.affiliation: Sorghum Breeding, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Andhra Pradesh 502 324, India.
authors.forename: Are Ashok
authors.initials: AA
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Science campus, Alagappa University, Karaikudi, Tamil Nadu 630 004, India. Electronic address: mrbiotech.alu@gmail.com.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Ramesh
mesh_terms: D014644:Genetic Variation; D005838:Genotype; D019105:Random Amplified Polymorphic DNA Technique; D045868:Sorghum / Q000235:genetics*
keywords: DAMD; Genetic variation; ISSR; RAPD; SPAR methods; Sorghum