title: High-Speed Super-Resolution Imaging of Live Fission Yeast Cells.
abstract: We describe a step-by-step method for high-speed fluorescence photoactivation localization microscopy (FPALM) of live fission yeast cells. The resolution with this method is tenfold better than spinning disk confocal microscopy.
authors.affiliation: Department of Molecular Cellular and Developmental Biology, Yale University, 208103, New Haven, CT, 06520-8103, USA.
authors.forename: Caroline
authors.initials: C
authors.lastname: Laplante
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA.|Department of Biomedical Engineering, Yale University, 208103, New Haven, CT, 06520-8103, USA.
authors.forename: Joerg
authors.initials: J
authors.lastname: Bewersdorf
authors.affiliation: Department of Molecular Cellular and Developmental Biology, Yale University, 208103, New Haven, CT, 06520-8103, USA. thomas.pollard@yale.edu.|Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA. thomas.pollard@yale.edu.|Department of Molecular Biophysics and Biochemistry, Yale University, 208103, New Haven, CT, 06520-8103, USA. thomas.pollard@yale.edu.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Pollard
mesh_terms: D018390:Gene Targeting; D008856:Microscopy, Fluorescence / Q000379:methods*; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics; D014170:Transformation, Genetic
keywords: FPALM; Fission yeast; Fluorescence microscopy; Live cell s; Super-resolution