title: Glutathione S-Transferase M1 and T1 Gene Polymorphisms in Patients with Chronic Plaque-Type Psoriasis: A Case-Control Study.
abstract: OBJECTIVE
  To determine the role of glutathione S-transferase (GST) isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort.
  
  
  METHODS
  In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs.
  
  
  RESULTS
  Of the 150 patients with psoriasis, 83 (79%) were identified with the GSTT1 genotype and 22 (21%) with the null genotype. Of the 102 patients in the control group, 69 (67.6%) subjects were identified with the GSTT1 genotype and 33 (32.4%) with the null genotype. There was no significant difference between the patient and control groups (p = 0.063). Regarding the GSTM1 polymorphism, 54 (51.4%) patients were identified with this genotype and 51 (48.6%) with the null genotype; in the control group, 50 (49%) were identified with this genotype and 52 (51%) with the null genotype. Again there was no statistically significant difference between the groups (p = 0.957).
  
  
  CONCLUSIONS
  In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed.
authors.affiliation: Department of Dermatology, School of Medicine, Sakarya University, Sakarya, Turkey.
authors.forename: Berna
authors.initials: B
authors.lastname: Solak
authors.forename: Mutlu
authors.initials: M
authors.lastname: Karkucak
authors.forename: Hakan
authors.initials: H
authors.lastname: Turan
authors.forename: Gökhan
authors.initials: G
authors.lastname: Ocakoğlu
authors.forename: Şebnem
authors.initials: Ş
authors.lastname: Özemri Sağ
authors.forename: Esma
authors.initials: E
authors.lastname: Uslu
authors.forename: Tahsin
authors.initials: T
authors.lastname: Yakut
authors.forename: Teoman
authors.initials: T
authors.lastname: Erdem
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011565:Psoriasis / Q000235:genetics*; D012307:Risk Factors; D014421:Turkey
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1