title: Chemosensory proteins involved in host recognition in the stored-food mite Tyrophagus putrescentiae.
abstract: BACKGROUND
  Chemosensory proteins (CSPs) have been proposed to transport a range of aliphatic compounds, esters and other long-chain compounds. A large number of CSPs from different gene subfamilies have been identified and annotated in arthropods; however, the CSP genes in mites remain unknown. Tyrophagus putrescentiae Schrank is an important stored-product and house-dust pest.
  
  
  RESULTS
  By analysing the transcriptome, two putative CSPs were identified, namely TputCSP1 and TputCSP2 (14.9 kDa and 12.1 kDa respectively). The phylogenetic tree showed that the two TputCSPs shared most homology with CSPs in Ixodes scapularis and partially with Diptera, including Anopheles gambiae, Drosophila melanogaster, D. pseudoobscura, D. simulans, Delia antiqua and Culex quinquefasciatus. Additionally, they had similar secondary structure. The 3D models revealed that there are six α-helices enclosing the hydrophobic ligand binding pocket. Based on a docking study, we found that three ligands, (-)-alloaromadendrene, 2-methylnaphthalene and cyclopentadecane, had high binding affinities for TputCSP1. Moreover, the TputCSP2 protein had a higher inhibition constant with different affinities to all test ligands from host volatile substances.
  
  
  CONCLUSIONS
  The two CSPs have distinct physiological functions. TputCSP1 may mediate host recognition. © 2015 Society of Chemical Industry.
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Shao-Xuan
authors.initials: SX
authors.lastname: Qu
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Li
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, Jiangsu, China.
authors.forename: Jin-Di
authors.initials: JD
authors.lastname: Song
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Hong
mesh_terms: D040041:Acaridae / Q000378:metabolism*; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D005260:Female; D008024:Ligands; D008297:Male; D062105:Molecular Docking Simulation; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D060829:Arthropod Proteins; D008024:Ligands
keywords: acarid mite; chemosensory proteins; chemosensory recognition; olfactory system