title: [Progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes].
abstract: Cytochrome P450 (CYP) enzymes and uridine 5-diphospho-glucuronosyltransferase (UGT) enzymes are critical enzymes for drug metabolism. Both chemical drugs and traditional Chinese medicines are converted to more readily excreted compounds by drug metabolizing enzymes in human livers. Because of the disparate expression of CYP and UGT enzymes among different individuals, accurate quantification of these enzymes is essential for drug pharmacology, drug-drug interactions and drug clinical applications. The research progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes in the recent decade is reviewed.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Wang
authors.forename: Yayao
authors.initials: Y
authors.lastname: Lu
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002853:Chromatography, Liquid; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004347:Drug Interactions; D014453:Glucuronosyltransferase / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000201:enzymology*
chemical_list: D003577:Cytochrome P-450 Enzyme System; D014453:Glucuronosyltransferase