title: Mediated amperometry reveals different modes of yeast responses to sugars.
abstract: Menadione-mediated amperometry at carbon paste electrodes modified with various yeasts (Saccharomyces cerevisiae, Candida pulcherrima, Pichia guilliermondii and Debaryomyces hansenii) was employed to monitor redox activity inside the yeast cells induced by glucose, fructose, sucrose, maltose or galactose. Continuous measurements revealed distinct modes (transient or gradually increasing) of the current development during the first 2 to 3 min after subjection to glucose, fructose and sucrose at electrodes containing S. cerevisiae and non-Saccharomyces strains. Different modes (increasing or decreasing) of the current development after yeast subjection to galactose at electrodes with S. cerevisiae or D. hansenii and at electrodes with C. pulcherrima and P. guilliermondii suggested different mechanisms of galactose assimilation.
authors.affiliation: Center for Physical Sciences and Technology, Institute of Chemistry, Gostauto 9, 01108 Vilnius, Lithuania. Electronic address: rasa.garjonyte@chi.lt.
authors.forename: Rasa
authors.initials: R
authors.lastname: Garjonyte
authors.affiliation: Nature Research Center, Institute of Botany, Zaliuju ezeru 49, 08406 Vilnius, Lithuania.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Melvydas
authors.affiliation: Center for Physical Sciences and Technology, Institute of Chemistry, Gostauto 9, 01108 Vilnius, Lithuania.
authors.forename: Albertas
authors.initials: A
authors.lastname: Malinauskas
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D024483:Vitamin K 3 / Q000737:chemistry*; D015003:Yeasts / Q000145:classification / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D024483:Vitamin K 3
keywords: Mediated amperometry; Menadione; Sugars; Yeasts