title: Purification and Characterization of a New κ-Carrageenase from the Marine Bacterium Vibrio sp. NJ-2.
abstract: The carrageenan-degrading marine bacterium Vibrio sp. strain NJ-2 was isolated from rotten red algae, and κ-carrageenase with high activity was purified from the culture supernatant. The purified enzyme with molecular mass of 33 kDa showed the maximal activity of 937 U/mg at 40°C and pH 8.0. It maintained 80% of total activity below 40°C and between pH 6.0 and 10.0. The kinetics experiment showed the Km and Vmax values were 2.54 mg/ml and 138.89 mmol/min/mg, respectively. The thin layer chromatography and ESI-MS analysis of hydrolysates indicated that the enzyme can endolytically depolymerize the kappa-carrageenan into oligosaccharides with degrees of depolymerization of 2-8. Owing to its high activity, it could be a valuable tool to produce κ-carrageenan oligosaccharides with various biological activities.
authors.affiliation: Preclinical Medicine College, Nanjing University of Chinese Medicine, Nanjing 210023, P.R. China.|School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China.
authors.forename: Benwei
authors.initials: B
authors.lastname: Zhu
authors.affiliation: Preclinical Medicine College, Nanjing University of Chinese Medicine, Nanjing 210023, P.R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Ning
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002351:Carrageenan / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D010802:Phylogeny; D000461:Rhodophyta / Q000382:microbiology; D013696:Temperature; D014733:Vibrio / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D009844:Oligosaccharides; D002351:Carrageenan; D006026:Glycoside Hydrolases
keywords: characterization; oligosaccharides; purification; κ-carrageenase