title: Long Non Coding RNA MALAT1 Promotes Tumor Growth and Metastasis by inducing Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma.
abstract: The prognosis of advanced oral squamous cell carcinoma (OSCC) patients remains dismal, and a better understanding of the underlying mechanisms is critical for identifying effective targets with therapeutic potential to improve the survival of patients with OSCC. This study aims to clarify the clinical and biological significance of metastasis-associated long non-coding RNA, metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in OSCC. We found that MALAT1 is overexpressed in OSCC tissues compared to normal oral mucosa by real-time PCR. MALAT1 served as a new prognostic factor in OSCC patients. When knockdown by small interfering RNA (siRNA) in OSCC cell lines TSCCA and Tca8113, MALAT1 was shown to be required for maintaining epithelial-mesenchymal transition (EMT) mediated cell migration and invasion. Western blot and immunofluorescence staining showed that MALAT1 knockdown significantly suppressed N-cadherin and Vimentin expression but induced E-cadherin expression in vitro. Meanwhile, both nucleus and cytoplasm levels of β-catenin and NF-κB were attenuated, while elevated MALAT1 level triggered the expression of β-catenin and NF-κB. More importantly, targeting MALAT1 inhibited TSCCA cell-induced xenograft tumor growth in vivo. Therefore, these findings provide mechanistic insight into the role of MALAT1 in regulating OSCC metastasis, suggesting that MALAT1 is an important prognostic factor and therapeutic target for OSCC.
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Su
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Genetics, Geisel School of Medicine at Dartmouth, Hanover, NH, 03755, USA.
authors.forename: Guoshuai
authors.initials: G
authors.lastname: Cai
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Lingping
authors.initials: L
authors.lastname: Kong
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ren
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Tianjin Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Mei
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute &Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000235:genetics*; D016328:NF-kappa B / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D051176:beta Catenin / Q000235:genetics
chemical_list: C557041:MALAT1 long non-coding RNA, human; D016328:NF-kappa B; D062085:RNA, Long Noncoding; D051176:beta Catenin