title: Influence of fucoidans and their derivatives on antitumor and phagocytic activity of human blood leucocytes.
abstract: The immunotropic activity of structurally different fucoidans and their derivatives towards isolated immune blood cells, effectors of innate immune system, was studied. The most potent effect was observed for high molecular weight fucoidan CF from the alga Chordaria flagelliformis, whose backbone is built of (1→3)-linked units of α-L-fucopyranose, and branches included residues of α-D-glucuronic acid and α-L-fucofuranose. This compound at the concentration of 0.05 mg/ml potentiated phagocytosis of Saccharomyces cerevisiae and Lactobacillus acidophilus by neutrophils, increasing relative quantity of phagocytes as well as their effectiveness. Along with this, 14% increase in the concentration of membrane-bound integrin CD11c molecules was observed. The systemic effect of CF at the dose of 0.01 mg/mouse i.p. led to potentiation of cytotoxic activity of spleen mononuclear leucocytes towards melanoma cells of line B16 by 1.9-fold and towards chronic myelogenous leukemia cells of line K-562 by 1.7-fold. These results indicate that fucoidan CF can stimulate anti-infective and antitumor activity of effectors of the innate immune system via CD11c integrins.
authors.affiliation: Blokhin Russian Cancer Research Center, Moscow, 115478, Russia. kisele@inbox.ru.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Anisimova
authors.forename: N E
authors.initials: NE
authors.lastname: Ustyuzhanina
authors.forename: F V
authors.initials: FV
authors.lastname: Donenko
authors.forename: M I
authors.initials: MI
authors.lastname: Bilan
authors.forename: N A
authors.initials: NA
authors.lastname: Ushakova
authors.forename: A I
authors.initials: AI
authors.lastname: Usov
authors.forename: N E
authors.initials: NE
authors.lastname: Nifantiev
authors.forename: M V
authors.initials: MV
authors.lastname: Kiselevskiy
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010586:Phagocytes / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; C007789:fucoidan