title: Expression of Fatty Acid Synthase Negatively Correlates with PTEN and Predicts Peritoneal Dissemination of Human Gastric Cancer.
abstract: BACKGROUND
  This study aimed to examine the clinical significance of fatty acid synthase (FASN) expression in gastric cancer (GC), and investigate any prognostic role.
  
  
  METHODS
  FASN expression was assessed in gastric cancers by immunohistochemistry using 60 paraffin-embedded tissue specimens, and clinical data were collected by retrospective chart review. Moreover, FASN mRNA expression in 15 fresh resected specimens was evaluated by the reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical staining of PTEN was performed to assess the correlation of PTEN with FASN in gastric cancer.
  
  
  RESULTS
  Increased expression of FASN was noted in gastric cancers. The frequency of FASN gene amplification was also significantly higher in gastric cancer than in adjacent normal tissue. FASN expression in human gastric cancer tissues was significantly correlated with patient TNM stage and peritoneal dissemination (p<0.05). Moreover, higher FASN expression significantly correlated with shorter overall survival (p<0.05). Here, upregulation of FASN negatively correlated with PTEN expression in gastric cancer.
  
  
  CONCLUSIONS
  These findings indicate that FASN expression is upregulated in gastric cancer, and increased FASN may be critical to th peritoneal metastasis and survival. Our results suggest that FASN upregulation and PTEN downregualtion may be involved in peritoneal dissemination for gastric cancer progression.
authors.affiliation: Department of Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China E-mail : xinhuachenlei@126.com.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Xiang
authors.forename: Jun
authors.initials: J
authors.lastname: Hao
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
mesh_terms: D002277:Carcinoma / Q000737:chemistry* / Q000235:genetics / Q000556:secondary*; D015536:Down-Regulation; D054890:Fatty Acid Synthase, Type I / Q000032:analysis* / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase / Q000032:analysis*; D010534:Peritoneal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000556:secondary*; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology*; D015996:Survival Rate; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; C518729:FASN protein, human; D054890:Fatty Acid Synthase, Type I; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human