title: The utilization of natural soda resource of Ordos in the cultivation of Nannochloropsis oceanica.
abstract: Nannochloropsis oceanica is famous for its strong environmental adaptability and oil-richness, especially high eicosapentaenoic acid (EPA) content. In this report, the possibility and cultivation parameters for N. oceanica using natural crude soda were testified and compared with seawater culture. To keep a suitable salinity range, different ratio of sea salt addition into soda lake water were used and the growth, lipid content, Fv/Fm and fatty acids profiling were inspected with nitrogen repletion or depletion. The results showed the best performance were achieved while 18g/L (salinity 25‰) sea salt was added into crude soda solution. The μmax and EPA content in fatty acids were 0.72/0.42 and 36%/23% in 500mL/100L bioreactor cultivations respectively, which maintained a relative high productivity to other reports. By comparing the growth and operations with Spirulina production, the feasibility of N. Oceanica in Ordos was proved on both technical and economical point of view.
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China; Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Yang
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Peili
authors.initials: P
authors.lastname: Shen
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China.
authors.forename: Peichun
authors.initials: P
authors.lastname: Wu
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China. Electronic address: c_x_p@dicp.ac.cn.
authors.forename: Xupeng
authors.initials: X
authors.lastname: Cao
authors.affiliation: Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D004952:Esters; D005227:Fatty Acids / Q000096:biosynthesis* / Q000737:chemistry; D005285:Fermentation; D060106:Lakes; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000254:growth & development; D009584:Nitrogen / Q000737:chemistry*; D054712:Salinity; D012492:Salts; D012623:Seawater; D053545:Spirulina / Q000254:growth & development; D058009:Stramenopiles / Q000254:growth & development*
chemical_list: D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D012492:Salts; D002244:Carbon; D015118:Eicosapentaenoic Acid; D009584:Nitrogen
keywords: EPA; Fatty acids; Nannochloropsis oceanica; Natural soda lake water; Ordos