title: [Determination of the Plant Origin of Licorice Oil Extract, a Natural Food Additive, by Principal Component Analysis Based on Chemical Components].
abstract: "Licorice oil extract" (LOE) (antioxidant agent) is described in the notice of Japanese food additive regulations as a material obtained from the roots and/or rhizomes of Glycyrrhiza uralensis, G. inflata or G. glabra. In this study, we aimed to identify the original Glycyrrhiza species of eight food additive products using LC/MS. Glabridin, a characteristic compound in G. glabra, was specifically detected in seven products, and licochalcone A, a characteristic compound in G. inflata, was detected in one product. In addition, Principal Component Analysis (PCA) (a kind of multivariate analysis) using the data of LC/MS or (1)H-NMR analysis was performed. The data of thirty-one samples, including LOE products used as food additives, ethanol extracts of various Glycyrrhiza species and commercially available Glycyrrhiza species-derived products were assessed. Based on the PCA results, the majority of LOE products was confirmed to be derived from G. glabra. This study suggests that PCA using (1)H-NMR analysis data is a simple and useful method to identify the plant species of origin of natural food additive products.
authors.affiliation: National Institute of Health Sciences.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Tada
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ishizuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Kayo
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kawahara
authors.forename: Takako
authors.initials: T
authors.lastname: Suematsu
authors.forename: Kazunori
authors.initials: K
authors.lastname: Arifuku
authors.forename: Toshio
authors.initials: T
authors.lastname: Fukai
authors.forename: Yukiyoshi
authors.initials: Y
authors.lastname: Tamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
authors.forename: Maiko
authors.initials: M
authors.lastname: Tahara
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
mesh_terms: D047188:Chalcones / Q000032:analysis; D002853:Chromatography, Liquid; D000431:Ethanol; D005503:Food Additives / Q000737:chemistry*; D006035:Glycyrrhiza / Q000737:chemistry* / Q000145:classification*; D007529:Isoflavones / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011522:Protons
chemical_list: D047188:Chalcones; D005503:Food Additives; D007529:Isoflavones; D010636:Phenols; D010936:Plant Extracts; D010938:Plant Oils; D011522:Protons; D000431:Ethanol; C107601:glabridin; C070840:licochalcone A