title: Resistance training minimizes catabolic effects induced by sleep deprivation in rats.
abstract: Sleep deprivation (SD) can induce muscle atrophy. We aimed to investigate the changes underpinning SD-induced muscle atrophy and the impact of this condition on rats that were previously submitted to resistance training (RT). Adult male Wistar EPM-1 rats were randomly allocated into 1 of 5 groups: control, sham, SD (for 96 h), RT, and RT+SD. The major outcomes of this study were muscle fiber cross-sectional area (CSA), anabolic and catabolic hormone profiles, and the abundance of select proteins involved in muscle protein synthesis and degradation pathways. SD resulted in muscle atrophy; however, when SD was combined with RT, the reduction in muscle fiber CSA was attenuated. The levels of IGF-1 and testosterone were reduced in SD animals, and the RT+SD group had higher levels of these hormones than the SD group. Corticosterone was increased in the SD group compared with the control group, and this increase was minimized in the RT+SD group. The increases in corticosterone concentrations paralleled changes in the abundance of ubiquitinated proteins and the autophagic proteins LC3 and p62/SQSTM1, suggesting that corticosterone may trigger these changes. SD induced weight loss, but this loss was minimized in the RT+SD group. We conclude that SD induced muscle atrophy, probably because of the increased corticosterone and catabolic signal. High-intensity RT performed before SD was beneficial in containing muscle loss induced by SD. It also minimized the catabolic signal and increased synthetic activity, thereby minimizing the body's weight loss.
authors.affiliation: a Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.|b Laboratório Interdisciplinar em Fisiologia e Exercício, São Paulo, Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Mônico-Neto
authors.affiliation: b Laboratório Interdisciplinar em Fisiologia e Exercício, São Paulo, Brazil.|c Department of Biosciences, Universidade Federal de São Paulo, Santos, Brazil.
authors.forename: Hanna Karen Moreira
authors.initials: HK
authors.lastname: Antunes
authors.affiliation: d Department of Biochemistry, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Kil Sun
authors.initials: KS
authors.lastname: Lee
authors.affiliation: e Department of Kinesiology, McMaster University, Hamilton, ON, Canada.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Phillips
authors.affiliation: b Laboratório Interdisciplinar em Fisiologia e Exercício, São Paulo, Brazil.|c Department of Biosciences, Universidade Federal de São Paulo, Santos, Brazil.
authors.forename: Sara Quaglia de Campos
authors.initials: SQ
authors.lastname: Giampá
authors.affiliation: a Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.|b Laboratório Interdisciplinar em Fisiologia e Exercício, São Paulo, Brazil.
authors.forename: Helton de Sá
authors.initials: Hde S
authors.lastname: Souza
authors.affiliation: a Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.|b Laboratório Interdisciplinar em Fisiologia e Exercício, São Paulo, Brazil.
authors.forename: Murilo
authors.initials: M
authors.lastname: Dáttilo
authors.affiliation: c Department of Biosciences, Universidade Federal de São Paulo, Santos, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Medeiros
authors.affiliation: c Department of Biosciences, Universidade Federal de São Paulo, Santos, Brazil.
authors.forename: Wilson Max
authors.initials: WM
authors.lastname: de Moraes
authors.affiliation: a Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
authors.affiliation: a Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.|f School of Physical Education, Physiotherapy and Occupational Therapy, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Marco Túlio
authors.initials: MT
authors.lastname: de Mello
mesh_terms: D000818:Animals; D001343:Autophagy; D003345:Corticosterone / Q000378:metabolism; D004195:Disease Models, Animal; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009133:Muscular Atrophy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D017208:Rats, Wistar; D055070:Resistance Training; D015398:Signal Transduction; D012892:Sleep Deprivation / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D013739:Testosterone / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D009124:Muscle Proteins; C548904:insulin-like growth factor-1, rat; D013739:Testosterone; D007334:Insulin-Like Growth Factor I; D046988:Proteasome Endopeptidase Complex; D003345:Corticosterone
keywords: atrophie musculaire; autophagie; autophagy; exercice contre résistance; muscle atrophy; perte de sommeil; proteasome; protéasome; resistance exercise; sleep loss