title: Immunohistochemical expression of matrix metalloproteinase-1, matrix metalloproteinase-2 and matrix metalloproteinase-9, myofibroblasts and Ki-67 in actinic cheilitis and lip squamous cell carcinoma.
abstract: Matrix metalloproteinases (MMPs), myofibroblasts (MFs) and epithelial proliferation have key roles in neoplastic progression. In this study immunoexpression of MMP-1, MMP-2 and MMP-9, presence of MFs and the epithelial proliferation index were investigated in actinic cheilitis (AC), lip squamous cell carcinoma (LSCC) and mucocele (MUC). Thirty cases of AC, thirty cases of LSCC and twenty cases of MUC were selected for immunohistochemical investigation of the proteins MMP-1, MMP-2, MMP-9, α-smooth muscle actin (α-SMA) and Ki-67. The MMP-1 expression in the epithelial component was higher in the AC than the MUC and LSCC. In the connective tissue, the expression was higher in the LSCC. MMP-2 showed lower epithelial and stromal immunostaining in the LSCC when compared to the AC and MUC. The epithelial staining for MMP-9 was higher in the AC when compared to the LSCC. However, in the connective tissue, the expression was lower in the AC compared to other lesions. The cell proliferation rate was increased in proportion to the severity of dysplasia in the AC, while in the LSCC it was higher in well-differentiated lesions compared to moderately differentiated. There were no statistically significant differences in number of MFs present in the lesions studied. The results suggest that MMPs could affect the biological behaviour of ACs and LSCCs inasmuch as they could participate in the development and progression from premalignant lesions to malignant lesions.
authors.affiliation: Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Bianca C
authors.initials: BC
authors.lastname: Bianco
authors.affiliation: Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Scotti
authors.affiliation: Pathology Department, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Daniella S C
authors.initials: DS
authors.lastname: Vieira
authors.affiliation: Morphology Sciences Department and Dentistry Graduate Program, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Michelle T
authors.initials: MT
authors.lastname: Biz
authors.affiliation: Dentistry Department, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Renata G
authors.initials: RG
authors.lastname: Castro
authors.affiliation: Pathology Department and Dentistry Graduate Program, Federal University of Santa Catarina, Florianopolis, Brazil.
authors.forename: Filipe
authors.identifier: http://orcid.org/0000-0002-5395-0486
authors.initials: F
authors.lastname: Modolo
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002613:Cheilitis / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008048:Lip Neoplasms / Q000378:metabolism / Q000473:pathology*; D020781:Matrix Metalloproteinase 1 / Q000032:analysis / Q000096:biosynthesis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000096:biosynthesis; D020782:Matrix Metalloproteinases / Q000032:analysis / Q000096:biosynthesis*; D058628:Myofibroblasts / Q000473:pathology*; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*
chemical_list: D019394:Ki-67 Antigen; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020781:Matrix Metalloproteinase 1
keywords: actinic cheilitis; cell proliferation; immunohistochemistry; lip cancer; matrix metalloproteinase-1; matrix metalloproteinase-2; matrix metalloproteinase-9; myofibroblasts; oral cancer; squamous cell carcinoma