title: Facile Synthesis of Biocompatible Fluorescent Nanoparticles for Cellular Imaging and Targeted Detection of Cancer Cells.
abstract: In this work, we report the facile synthesis of functional core-shell structured nanoparticles with fluorescence enhancement, which show specific targeting of cancer cells. Biopolymer poly-l-lysine was used to coat the silver core with various shell thicknesses. Then, the nanoparticles were functionalized with folic acid as a targeting agent for folic acid receptor. The metal-enhanced fluorescence effect was observed when the fluorophore (5-(and-6)-carboxyfluorescein-succinimidyl ester) was conjugated to the modified nanoparticle surface. Cellular imaging assay of the nanoparticles in folic acid receptor-positive cancer cells showed their excellent biocompatibility and selectivity. The as-prepared functional nanoparticles demonstrate the efficiency of the metal-enhanced fluorescence effect and provide an alternative approach for the cellular imaging and targeting of cancer cells.
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, P. R. China.
authors.forename: Fu
authors.initials: F
authors.lastname: Tang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, P. R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, P. R. China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, P. R. China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Li
mesh_terms: D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis*; D012834:Silver / Q000737:chemistry / Q000633:toxicity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D012834:Silver
keywords: cellular imaging; core−shell nanoparticles; fluorescence; fluorescence enhancement; polymer