title: Dispersion Behaviour of Silica Nanoparticles in Biological Media and Its Influence on Cellular Uptake.
abstract: Given the increasing variety of manufactured nanomaterials, suitable, robust, standardized in vitro screening methods are needed to study the mechanisms by which they can interact with biological systems. The in vitro evaluation of interactions of nanoparticles (NPs) with living cells is challenging due to the complex behaviour of NPs, which may involve dissolution, aggregation, sedimentation and formation of a protein corona. These variable parameters have an influence on the surface properties and the stability of NPs in the biological environment and therefore also on the interaction of NPs with cells. We present here a study using 30 nm and 80 nm fluorescently-labelled silicon dioxide NPs (Rubipy-SiO2 NPs) to evaluate the NPs dispersion behaviour up to 48 hours in two different cellular media either supplemented with 10% of serum or in serum-free conditions. Size-dependent differences in dispersion behaviour were observed and the influence of the living cells on NPs stability and deposition was determined. Using flow cytometry and fluorescence microscopy techniques we studied the kinetics of the cellular uptake of Rubipy-SiO2 NPs by A549 and CaCo-2 cells and we found a correlation between the NPs characteristics in cell media and the amount of cellular uptake. Our results emphasize how relevant and important it is to evaluate and to monitor the size and agglomeration state of nanoparticles in the biological medium, in order to interpret correctly the results of the in vitro toxicological assays.
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Blanka
authors.initials: B
authors.lastname: Halamoda-Kenzaoui
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Ceridono
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Pascal
authors.initials: P
authors.lastname: Colpo
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Valsesia
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Patricia
authors.initials: P
authors.lastname: Urbán
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Isaac
authors.initials: I
authors.lastname: Ojea-Jiménez
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gioria
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Douglas
authors.initials: D
authors.lastname: Gilliland
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: François
authors.initials: F
authors.lastname: Rossi
authors.affiliation: Nanobiosciences Unit, Institute for Health and Consumer Protection, European Commission Joint Research Centre (JRC), via E. Fermi 2749, 21027 Ispra, (VA), Italy.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kinsner-Ovaskainen
mesh_terms: D018938:Caco-2 Cells; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry*; D005434:Flow Cytometry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry / Q000493:pharmacokinetics*; D013499:Surface Properties
chemical_list: D003470:Culture Media; D012822:Silicon Dioxide