title: Brassinosteroid (BR) biosynthetic gene lhdd10 controls late heading and plant height in rice (Oryza sativa L.).
abstract: A Brd2 allele suppresses heading date by altering the expression of heading date regulators such as OsMADS50 , and also negatively regulates chlorophyll biosynthesis. Heading date and plant height are important determinants of yield in rice (Oryza sativa L.). In this study, we characterized a late heading, dwarf mutant known as lhdd10 selected following ethyl methane sulfonate (EMS)-treatment of ssp. indica cultivar 93-11. lhdd10 showed late heading, dwarfness and slightly darker-green leaves than wild-type 93-11 under long-day and short-day conditions. We isolated lhdd10 by map-based cloning; it encoded a putative FAD-linked oxidoreductase protein (a brassinosteroid biosynthetic gene) that localized to the nucleus. LHDD10 was constitutively expressed in various tissues, but more so in shoot apices and panicles. Our data showed that lhdd10 influences heading date by controlling the expression of heading date regulators, such as OsMADS50 in both LD and SD conditions. lhdd10 also negatively regulated expression of chlorophyll biosynthetic genes to reduce the chlorophyll content. Our data indicated that BRs play important roles in regulating heading date and chlorophyll biosynthesis. This work provides material that will allow study of how BRs regulate heading date in rice.
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Feng
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Zhou
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: C L
authors.initials: CL
authors.lastname: Mou
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: T
authors.initials: T
authors.lastname: Wu
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. jiangling@njau.edu.cn.
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China. wanjm@njau.edu.cn.|National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. wanjm@njau.edu.cn.
authors.forename: J M
authors.initials: JM
authors.lastname: Wan
mesh_terms: D060406:Brassinosteroids / Q000096:biosynthesis*; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics* / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D060406:Brassinosteroids; D010940:Plant Proteins; D002734:Chlorophyll
keywords: Brassinosteroid (BRs); Chlorophyll biosynthesis; Dwarfism; Heading date