title: Ligand Replacement Approach to Raman-Responded Molecularly Imprinted Monolayer for Rapid Determination of Penicilloic Acid in Penicillin.
abstract: Penicilloic acid (PA) is a degraded byproduct of penicillin and often causes fatal allergies to humans, but its rapid detection in penicillin drugs remains a challenge due to its similarity to the mother structure of penicillin. Here, we reported a ligand-replaced molecularly imprinted monolayer strategy on a surface-enhanced Raman scattering (SERS) substrate for the specific recognition and rapid detection of Raman-inactive PA in penicillin. The bis(phenylenediamine)-Cu(2+)-PA complex was first synthesized and stabilized onto the surface of silver nanoparticle film that was fabricated by a bromide ion-added silver mirror reaction. A molecularly imprinted monolayer was formed by the further modification of alkanethiol around the stabilized complex on the Ag film substrate, and the imprinted recognition site was then created by the replacement of the complex template with Raman-active probe molecule p-aminothiophenol. When PA rebound into the imprinted site in the alkanethiol monolayer, the SERS signal of p-aminothiophenol exhibited remarkable enhancement with a detection limit of 0.10 nM. The imprinted monolayer can efficiently exclude the interference of penicillin and thus provides a selective determination of 0.10‰ (w/w) PA in penicillin, which is about 1 order of magnitude lower than the prescribed residual amount of 1.0‰. The strategy reported here is simple, rapid and inexpensive compared to the traditional chromatography-based methods.
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
authors.forename: Shuhu
authors.initials: S
authors.lastname: Du
authors.affiliation: Institute of Intelligent Machines, Chinese Academy of Sciences , Hefei, Anhui 230031, China.|State Key Laboratory of Transducer Technology, Chinese Academy of Sciences , Hefei, Anhui 230031, China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D008024:Ligands; D054802:Molecular Imprinting; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D010406:Penicillins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013997:Time Factors
chemical_list: D008024:Ligands; D010406:Penicillins; C008782:penicilloic acid; D010397:Penicillanic Acid