title: Optimizing Multiple Analyte Injections in Surface Plasmon Resonance Biosensors with Analytes having Different Refractive Index Increments.
abstract: Surface plasmon resonance-based biosensors have been successfully applied to the study of the interactions between macromolecules and small molecular weight compounds. In an effort to increase the throughput of these SPR-based experiments, we have already proposed to inject multiple compounds simultaneously over the same surface. When specifically applied to small molecular weight compounds, such a strategy would however require prior knowledge of the refractive index increment of each compound in order to correctly interpret the recorded signal. An additional experiment is typically required to obtain this information. In this manuscript, we show that through the introduction of an additional global parameter corresponding to the ratio of the saturating signals associated with each molecule, the kinetic parameters could be identified with similar confidence intervals without any other experimentation.
authors.affiliation: Department of Chemical Engineering, École Polytechnique de Montréal. P.O. Box 6079, Centre-ville Station, H3C 3A7 Montréal, Québec, Canada.
authors.forename: Massinissa Si
authors.initials: MS
authors.lastname: Mehand
authors.affiliation: Department of Chemical Engineering, École Polytechnique de Montréal. P.O. Box 6079, Centre-ville Station, H3C 3A7 Montréal, Québec, Canada.
authors.forename: Bala
authors.initials: B
authors.lastname: Srinivasan
authors.affiliation: Department of Chemical Engineering, École Polytechnique de Montréal. P.O. Box 6079, Centre-ville Station, H3C 3A7 Montréal, Québec, Canada.
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
mesh_terms: D015374:Biosensing Techniques; D007700:Kinetics; D008962:Models, Theoretical; D008970:Molecular Weight; D012031:Refractometry; D013449:Sulfonamides / Q000032:analysis / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D013449:Sulfonamides