title: Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative.
abstract: Dental caries, a biofilm-related oral disease, is a result of disruption of the microbial ecological balance in the oral environment. Streptococcus mutans, which is one of the primary cariogenic bacteria, produces glucosyltransferases (Gtfs) that synthesize extracellular polysaccharides (EPSs). The EPSs, especially water-insoluble glucans, contribute to the formation of dental plaque, biofilm stability, and structural integrity, by allowing bacteria to adhere to tooth surfaces and supplying the bacteria with protection against noxious stimuli and other environmental attacks. The identification of novel alternatives that selectively inhibit cariogenic organisms without suppressing oral microbial residents is required. The goal of the current study is to investigate the influence of an oxazole derivative on S. mutans biofilm formation and the development of dental caries in rats, given that oxazole and its derivatives often exhibit extensive and pharmacologically important biological activities. Our data shows that one particular oxazole derivative, named 5H6, inhibited the formation of S. mutans biofilms and prevented synthesis of extracellular polysaccharides by antagonizing Gtfs in vitro, without affecting the growth of the bacteria. In addition, topical applications with the inhibitor resulted in diminished incidence and severity of both smooth and sulcal surface caries in vivo with a lower percentage of S. mutans in the animals' dental plaque compared to the control group (P < 0.05). Our results showed that this oxazole derivative has the capacity to inhibit biofilm formation and cariogenicity of S. mutans.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China.|Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China.|Department of Endodontics Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Structural Biology, Key Laboratory of Applied and Environmental Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, People's Republic of China.
authors.forename: Jumei
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China. zoujing1970@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, People's Republic of China. liyuqing@scu.edu.cn.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Li
mesh_terms: D000631:Aminopyridines / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors; D010080:Oxazoles / Q000737:chemistry / Q000494:pharmacology*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D051381:Rats; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence / Q000187:drug effects
chemical_list: C000611843:5H6 oxazolone; D000631:Aminopyridines; D005936:Glucans; D010080:Oxazoles; D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases
keywords: Biofilm; Dental caries; Extracellular polysaccharides; Glucosyltransferase; Oxazole derivative; Streptococcus mutans