title: Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2'-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay.
abstract: Snow chrysanthemum (Coreopsis tinctoria Nutt.), a world-widely well-known flower tea material, has attracted more and more attention because of its beneficial health effects such as antioxidant activity and special flavor. In this study, a high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) and 2,2'-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt (ABTS) based assay was employed for comparison and identification of antioxidants in different samples of snow chrysanthemum. The results showed that snow chrysanthemum flowers possessed the highest while stems presented the lowest antioxidant capacities. Fourteen detected peaks with antioxidant activity were temporarily identified as 3,4',5,6,7-pentahydroxyflavanone-O-hexoside, chlorogenic acid, 2R-3',4',8-trihydroxyflavanone-7-O-glucoside, flavanomarein, flavanocorepsin, flavanokanin, quercetagitin-7-O-glucoside, 3',5,5',7-tetrahydroxyflavanone-O-hexoside, marein, maritimein, 1,3-dicaffeoylquinic acid, coreopsin, okanin and acetyl-marein by comparing their UV spectra, retention times and MS data with standards or literature data. Antioxidants existed in snow chrysanthemum are quite different from those reported in Chrysanthemum morifolium, a well-known traditional beverage in China, which indicated that snow chrysanthemum may be a promising herbal tea material with obvious antioxidant activity.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: L X
authors.initials: LX
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: D J
authors.initials: DJ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: S C
authors.initials: SC
authors.lastname: Lam
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao.
authors.forename: L
authors.initials: L
authors.lastname: Ge
authors.affiliation: China Science and Technology Exchange Center, Beijing 100045, China.
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao. Electronic address: zhaojing.cpu@163.com.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Urumqi 830011, China.
authors.forename: Z R
authors.initials: ZR
authors.lastname: Long
authors.affiliation: Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute, Urumqi 830011, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Urumqi Jiangqi Agriculture Development Co. Ltd., Urumqi 830011, China.
authors.forename: B
authors.initials: B
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao. Electronic address: lishaoping@hotmail.com.
authors.forename: S P
authors.initials: SP
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002681:China; D002851:Chromatography, High Pressure Liquid; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry; D013058:Mass Spectrometry; D018547:Plant Stems / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: C487840:1,3-dicaffeoylquinic acid; D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D013451:Sulfonic Acids; D011801:Quinic Acid
keywords: Antioxidant activity; Coreopsis tinctoria; HPLC; Online ABTS-based assay