title: Study on the homology of the genomes of tetraploid Asiatic lilies (Lilium) using FISH.
abstract: Asiatic lily cultivars, bred by hybridization and (or) chromosome doubling of species of section Sinomartagon of Lilium, are diploid, triploid, or tetraploid, but the homology of the genomes among species of section Sinomartagon and Asiatic lilies remains unclear. In the present research, two tetraploid Asiatic cultivars were analyzed, using 45S rDNA as probe, for their FISH karyotypes and their chromosomal association, anaphase I, telophase II, and pollen viability were surveyed to assess the multivalent segregation. Chromosomal assortment of six progenies of the two tetraploid cultivars were also investigated. The results showed that the tetraploid cultivars had similar FISH karyotypes, they predominantly formed multivalents, and these were equally separated because their anaphase I, telophase II, and pollen viability were similar to those of diploid species. Apart from minor variations, FISH karyotypes of progenies were similar to each other and to their parents. Based on these results and considering the high crossability among species of section Sinomartagon and (or) Asiatic lilies, we concluded that species of section Sinomartagon and their resulting cultivars share a common genome; thus, polyploidy Asiatic lilies are autopolyploid.
authors.affiliation: a College of Forestry, Jiangxi Agricultural University, 1101 of Zhimindayao, Nanchang 330045, Jiangxi Province, China.|b Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhou
authors.affiliation: a College of Forestry, Jiangxi Agricultural University, 1101 of Zhimindayao, Nanchang 330045, Jiangxi Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.affiliation: a College of Forestry, Jiangxi Agricultural University, 1101 of Zhimindayao, Nanchang 330045, Jiangxi Province, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a College of Forestry, Jiangxi Agricultural University, 1101 of Zhimindayao, Nanchang 330045, Jiangxi Province, China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Xu
authors.affiliation: a College of Forestry, Jiangxi Agricultural University, 1101 of Zhimindayao, Nanchang 330045, Jiangxi Province, China.
authors.forename: Xuxin
authors.initials: X
authors.lastname: Liu
authors.affiliation: b Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Kehu
authors.initials: K
authors.lastname: Li
authors.affiliation: b Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China.
authors.forename: Guixue
authors.initials: G
authors.lastname: Zhou
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D004275:DNA, Ribosomal / Q000235:genetics; D004171:Diploidy; D018745:Genome, Plant; D006824:Hybridization, Genetic; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D027762:Lilium / Q000235:genetics*; D018519:Meristem / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D000069600:Plant Breeding; D011058:Pollen; D057891:Tetraploidy
chemical_list: D004275:DNA, Ribosomal
keywords: 45S rDNA; ADNr 45S; autotetraploid; autotétraploïde; biological species; espèce biologique; homologous; homologue; multivalent