title: A Quinoline-Based Ratiometric and Reversible Fluorescent Probe for Cadmium Imaging in Living Cells.
abstract: We report a novel ratiometric and reversible fluorescent probe for Cd(2+) detection utilizing a 6-(dimethylamino)quinaldine derivative as the fluorophore and a 2-hydrazinopyridine derivative as Cd(2+) chelator. This ratiometric fluorescent probe possesses favorable photophysical properties. It shows a large (55 nm) red-shift from 515 nm to 570 nm in the emission spectrum. Moreover, this probe also exhibits an excellent linear relationship of fluorescence intensity ratio (F570/F515) (R(2)=0.989) vs. Cd(2+) concentration in the range of 0-10 µM at physiological pH, which can serve as a "quantitative detecting" probe for Cd(2+). Utilizing this sensitive and selective probe, we have successfully detected Cd(2+) in living cells.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Sun
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Yuan
authors.forename: Jun-Fa
authors.initials: JF
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.forename: Ming-li
authors.initials: ML
authors.lastname: Zhu
authors.forename: Jigar A
authors.initials: JA
authors.lastname: Makawana
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Zhu
mesh_terms: D002104:Cadmium / Q000032:analysis*; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008744:Methylamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D015394:Molecular Structure; D011728:Pyridones / Q000737:chemistry; D011789:Quantum Theory; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: C000610325:6-(dimethylamino)quinaldine; D005456:Fluorescent Dyes; D008744:Methylamines; D011728:Pyridones; D011804:Quinolines; D002104:Cadmium; C062607:2-hydrazinopyridine