title: Evolution and mutations of hepatitis B virus quasispecies in genotype B and C during vertical transmission.
abstract: Evolution patterns of HBV QS between genotype B and C during vertical transmission are not well understood. In this study, we enrolled 10 HBV infected mother-infant pairs (four pairs with genotype B, four pairs with genotype C, and two with co-infection) without anti-viral therapy. Serum HBV DNA of mothers and infants were sequenced, HBV QS complexity and diversity were analyzed, polymorphisms and mutation sites were recorded, and phylogenetic trees were performed. Our result showed that the QS complexities in P (amino acid), C/PreC (amino acid), and PreS1 (nucleotide) gene were significantly higher in mothers than in infants in pairs with genotype C (P < 0.05), however, full-length and other genes showed non-significant differences (P > 0.05). Unlike genotype C, QS complexity of P gene (nucleotide) was significantly higher in infants than in mothers (P < 0.05) in pairs with genotype B, similarly, QS complexities of full-length and other genes (except Pre S2) were also higher in infants than in mothers but without significant differences (P > 0.05). QS diversities of full-length and most genes in genotype B were comparable between mothers and their infants (P > 0.05), in pairs with genotype C, dS of P, X, RT genes, genetic distance of Pre S1 gene (amino acid) and dN of Pre S1 gene were significant higher in mothers than in infants (P < 0.05). Several HBV mutations correlated with immune escape, e antigen loss and drug resistance were observed in infants. The results indicated that differences of HBV QS evolution patterns between genotype B and C during vertical transmission might contribute to distinct prognosis.
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.|Cadre Ward Two, Wuhan General Hospital of Guangzhou Military Command, Wuhan, China.
authors.forename: Quanxin
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Gynecology and Obstetrics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yan
authors.affiliation: National Plateau Wetland Research Center, Southwest Forest University, Kunming, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Yue
authors.affiliation: Department of Gynecology and Obstetrics, The First People's Hospital, Zigong, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Deng
authors.affiliation: Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing, China.|The Chongqing Key Laboratory for Research of Infectious Diseases, Chongqing, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D060085:Coinfection; D004279:DNA, Viral / Q000097:blood; D024882:Drug Resistance, Viral / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000635:transmission* / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006513:Hepatitis B e Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000635:transmission* / Q000821:virology; D006801:Humans; D057131:Immune Evasion / Q000235:genetics; D007223:Infant; D018445:Infectious Disease Transmission, Vertical; D009035:Mothers; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011498:Protein Precursors / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen
keywords: HBV mutation; immune escape; perinantal; quasispecies; transmission