title: Interaction of BODIPY Dyes with the Blood Plasma Proteins.
abstract: Photophysical properties of several BODIPY-based fluorescent dyes were investigated in systems containing blood plasma biomolecules and in model system containing bovine serum albumin in terms of electronic absorption and fluorescence spectroscopy. The interaction between the investigated dyes and protein plasma components changes spectral characteristics of the dyes and leads to bathochromic and hypochromic absorption spectra shifts accompanied by changing of fluorescence intensity. The mechanism of fluorescence changing was defined in the terms of Stern-Volmer theory. It was shown that the static factor of molecular dye-biopolymers complex formation prevails at plasma protein concentration up to 1 g/l, while the higher viscosity range is characterized mainly by nonspecific fluorophore interactions. The increase of fluorescent characteristics of phenyl-substituted BODIPY in the presence of proteins caused by resonance energy transfer and change of physicochemical properties of the molecular environment of the fluorophore was shown for the first time.
authors.affiliation: Ivanovo State University of Chemistry and Technology, Ivanovo, Russia. ymarfin@gmail.com.
authors.forename: Yu S
authors.initials: YS
authors.lastname: Marfin
authors.affiliation: Ivanovo State Medical Academy, Ivanovo, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Aleksakhina
authors.affiliation: Ivanovo State University of Chemistry and Technology, Ivanovo, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Merkushev
authors.affiliation: Ivanovo State University of Chemistry and Technology, Ivanovo, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Rumyantsev
authors.affiliation: Ivanovo State Medical Academy, Ivanovo, Russia.
authors.forename: I K
authors.initials: IK
authors.lastname: Tomilova
mesh_terms: D000818:Animals; D002417:Cattle; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008968:Molecular Conformation; D011162:Porphobilinogen / Q000031:analogs & derivatives* / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: C538930:3-anilino difluoroboron dipyrromethene; D005456:Fluorescent Dyes; D012709:Serum Albumin; D011162:Porphobilinogen
keywords: Blood plasma; Bodipy; Intermolecular interactions; Photophysical properties; Stern-volmer equation