title: Protective mechanism of grape seed oil on carbon tetrachloride-induced brain damage in γ-irradiated rats.
abstract: This study investigated the possible beneficial effects of grape seed oil (GSO) on carbon tetrachloride (CCl4)-induced acute neurotoxicity in γ-irradiated rats. A statistical significant decrease in superoxide-dismutase (SOD), catalase (CAT), and glutathione-peroxidase (GPx) activities and reduced glutathione (GSH) content were exhibited. Further, a significant elevation in malondialdehyde (MDA), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and transforming growth factor-beta-1 (TGF-β1) levels was observed. Furthermore, xanthine oxidase (XO) and inducible nitric oxide synthase (iNOS) gene expression were elevated in the γ-irradiated animals treated with an acute dose of CCl4. The pretreatment of GSO exerts significant amelioration of the studied parameters. In conclusion, this study demonstrated that GSO has a neuroprotective effect against CCl4-induced brain injury in γ-irradiated rats, which is likely attributed to its ability to scavenge the free radicals, suppress the inflammatory responses, improve the activity of the antioxidant enzymes and inhibit the XO and iNOS gene expression levels.
authors.affiliation: Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Nasr City, Cairo, Egypt. Electronic address: afmismail@gmail.com.
authors.forename: Amel F M
authors.initials: AF
authors.lastname: Ismail
authors.affiliation: Health Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Nasr City, Cairo, Egypt.
authors.forename: Fatma S M
authors.initials: FS
authors.lastname: Moawed
authors.affiliation: Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, Nasr City, Cairo, Egypt.
authors.forename: Marwa Abdelhameed
authors.initials: MA
authors.lastname: Mohamed
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000528:radiation effects*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005720:Gamma Rays; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D027843:Vitis / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Acute brain damage; Grape seed oil; Inducible nitric oxide synthase gene expression; Lipid peroxidation; Nitric oxide; Xanthine oxidase