title: The expression of the Cuphea palustris thioesterase CpFatB2 in Yarrowia lipolytica triggers oleic acid accumulation.
abstract: The conversion of industrial by-products into high-value added compounds is a challenging issue. Crude glycerol, a by-product of the biodiesel production chain, could represent an alternative carbon source for the cultivation of oleaginous yeasts. Here, we developed five minimal synthetic glycerol-based media, with different C/N ratios, and we analyzed the production of biomass and fatty acids by Yarrowia lipolytica Po1g strain. We identified two media at the expense of which Y. lipolytica was able to accumulate ∼5 g L(-1) of biomass and 0.8 g L(-1) of fatty acids (0.16 g of fatty acids per g of dry weight). These optimized media contained 0.5 g L(-1) of urea or ammonium sulfate and 20 g L(-1) of glycerol, and were devoid of yeast extract. Moreover, Y. lipolytica was engineered by inserting the FatB2 gene, coding for the CpFatB2 thioesterase from Cuphea palustris, in order to modify the fatty acid composition towards the accumulation of medium-chain fatty acids. Contrary to the expected, the expression of the heterologous gene increased the production of oleic acid, and concomitantly decreased the level of saturated fatty acids.
authors.affiliation: Dept. of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.|CSGI, Dept. of Chemistry, University of Florence, Sesto Fiorentino (FI), Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Stefan
authors.affiliation: Dept. of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.|CSGI, Dept. of Chemistry, University of Florence, Sesto Fiorentino (FI), Italy.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hochkoeppler
authors.affiliation: Consiglio per La Ricerca in Agricoltura E L'analisi Dell'economia Agraria, Centro Di Ricerca per Le Colture Industriali (CRA-CIN), Bologna, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ugolini
authors.affiliation: Consiglio per La Ricerca in Agricoltura E L'analisi Dell'economia Agraria, Centro Di Ricerca per Le Colture Industriali (CRA-CIN), Bologna, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Lazzeri
authors.affiliation: Dept. of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Conte
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D018533:Biomass; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D031562:Cuphea / Q000201:enzymology; D005990:Glycerol / Q000378:metabolism; D060847:Metabolic Engineering; D019301:Oleic Acid / Q000096:biosynthesis* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D013869:Thiolester Hydrolases / Q000096:biosynthesis* / Q000378:metabolism; D025062:Yarrowia / Q000201:enzymology / Q000235:genetics
chemical_list: D003470:Culture Media; D010940:Plant Proteins; D019301:Oleic Acid; D002244:Carbon; C098243:FatB2 protein, Cuphea palustris; D013869:Thiolester Hydrolases; D005990:Glycerol; D000645:Ammonium Sulfate
keywords: Yarrowia lipolytica; crude glycerol; fatty acids; oleaginous yeast; pure glycerol; single cell oil