title: Safety and efficacy of allogeneic adipose tissue-derived mesenchymal stem cells for treatment of dogs with inflammatory bowel disease: Clinical and laboratory outcomes.
abstract: Mesenchymal stem cells (MSCs) have shown immunomodulatory and anti-inflammatory effects in experimental colitis, and promising clinical results have been obtained in humans with Crohn's disease and ulcerative colitis. The aim of this study was to determine the safety and feasibility of adipose tissue-derived MSC (ASC) therapy in dogs with inflammatory bowel disease (IBD). Eleven dogs with confirmed IBD received one ASC intravascular (IV) infusion (2 × 10(6) cells/kg bodyweight). The outcome measures were clinical response based on percentage reduction of the validated Clinical Inflammatory Bowel Disease Activity Index (CIBDAI) and Canine Chronic Enteropathy Clinical Activity Index (CCECAI), as well as normalisation of C-reactive protein (CRP), albumin, folate and cobalamin serum concentrations at day 42 post-treatment. The Wilcoxon test was used to compare variables before and after treatment. No acute reaction to ASC infusion and no side effects were reported during follow-up in any dog. Six weeks post-treatment, the CIBDAI and CCECAI decreased significantly and albumin, cobalamin and folate concentrations increased substantially. Differences in CRP concentrations pre- and post-treatment were not significant (P = 0.050). Clinical remission (defined by a reduction of initial CIBDAI and CCECAI >75%) occurred in 9/11 dogs at day 42. The two remaining dogs showed a partial response with reduction percentages of 69.2% and 71.4%. In conclusion, a single IV infusion of allogeneic ASCs was well tolerated and appeared to produce clinical benefits in dogs with severe IBD.
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, University of Extremadura, 10003 Cáceres, Spain. Electronic address: evama@unex.es.
authors.forename: E M
authors.initials: EM
authors.lastname: Pérez-Merino
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, University of Extremadura, 10003 Cáceres, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Usón-Casaús
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, University of Extremadura, 10003 Cáceres, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Zaragoza-Bayle
authors.affiliation: Veterinary Teaching Hospital, Veterinary Faculty, University of Extremadura, 10003 Cáceres, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Duque-Carrasco
authors.affiliation: Centauri Biotech Company, 15142 Arteixo, A Coruña, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Mariñas-Pardo
authors.affiliation: Centauri Biotech Company, 15142 Arteixo, A Coruña, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Hermida-Prieto
authors.affiliation: Department of Animal Medicine and Surgery, Veterinary Faculty, University of Extremadura, 10003 Cáceres, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Barrera-Chacón
authors.affiliation: Department of Health Animal Science and Food Safety, Section of Surgery, Veterinary Faculty, Via Celoria, 10. 20133 Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Gualtieri
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D005260:Female; D015212:Inflammatory Bowel Diseases / Q000628:therapy / Q000662:veterinary*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000662:veterinary*; D059630:Mesenchymal Stem Cells; D014184:Transplantation, Homologous / Q000662:veterinary; D016896:Treatment Outcome
keywords: Albumin; Canine; Clinical activity index; Inflammatory bowel disease; Mesenchymal stem cell; Treatment