title: Making a Hematopoietic Stem Cell.
abstract: Previous attempts to either generate or expand hematopoietic stem cells (HSCs) in vitro have involved either ex vivo expansion of pre-existing patient or donor HSCs or de novo generation from pluripotent stem cells (PSCs), comprising both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). iPSCs alleviated ESC ethical issues but attempts to generate functional mature hematopoietic stem and progenitor cells (HSPCs) have been largely unsuccessful. New efforts focus on directly reprogramming somatic cells into definitive HSCs and HSPCs. To meet clinical needs and to advance drug discovery and stem cell therapy, alternative approaches are necessary. In this review, we synthesize the strategies used and the key findings made in recent years by those trying to make an HSC.
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, NY, USA; Black Family Stem Cell Institute, Icahn School of Medicine, New York, NY, USA; The Graduate School of Biomedical Science, Icahn School of Medicine, New York, NY, USA.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Daniel
authors.affiliation: CNC - Center for Neuroscience and Cell Biology, University of Coimbra, UC Biotech Building, Biocant Park, 3060-197 Cantanhede, Portugal.
authors.forename: Carlos-Filipe
authors.initials: CF
authors.lastname: Pereira
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, NY, USA; Black Family Stem Cell Institute, Icahn School of Medicine, New York, NY, USA; Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine, New York, NY, USA.
authors.forename: Ihor R
authors.initials: IR
authors.lastname: Lemischka
authors.affiliation: Department of Developmental and Regenerative Biology, Icahn School of Medicine, New York, NY, USA; Black Family Stem Cell Institute, Icahn School of Medicine, New York, NY, USA. Electronic address: kateri.moore@mssm.edu.
authors.forename: Kateri A
authors.initials: KA
authors.lastname: Moore
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D053595:Embryonic Stem Cells / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology
keywords: hematopoiesis; hematopoietic stem cell; reprogramming; transcription factors