title: Role of leptin in body temperature regulation and lipid metabolism following splenectomy.
abstract: OBJECTIVE
  The physiological changes in serum triglycerides and body temperature that are induced by splenectomy are poorly understood. Therefore, the aim of this study was to investigate parameters related to lipid and glucose metabolism, as well as thermoregulation, in splenectomized mice.
  
  
  METHODS
  Splenectomized and sham-operated WT mice (C57Bl/6) and ob/ob mice were randomly divided and treated with a standard or high fat diet, and several metabolic parameters and the body temperature were investigated.
  
  
  RESULTS
  Splenectomy induced a significant increase in triglyceride levels regardless of the diet. It was found that the splenectomized WT mice showed greater serum leptin and insulin levels compared with the sham-operated mice. Additionally, the body temperatures of the splenectomized WT mice were greater than the body temperatures of the control animals regardless of diet; this result too was observed without any significant change in the temperature of the splenectomized ob/ob animals.
  
  
  CONCLUSIONS
  The results suggest that splenectomy interferes with serum triglyceride metabolism and body temperature regardless of the fat content in the diet and that leptin is involved in the regulation of body temperature related to splenectomy.
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil; Graduate Program on Physical Education and Health, Catholic University of Brasilia, Brasilia, Brazil.
authors.forename: T S
authors.initials: TS
authors.lastname: Rosa
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil.
authors.forename: C E N
authors.initials: CE
authors.lastname: Amorim
authors.affiliation: University Federal of Pelotas, UFPEL, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Barros
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Haro
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Wasinski
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil.
authors.forename: F J
authors.initials: FJ
authors.lastname: Russo
authors.affiliation: School of Arts Sciences and Humanities, USP, Brazil.
authors.forename: R F P
authors.initials: RF
authors.lastname: Bacurau
authors.affiliation: Department of Biophysics, University Federal of Sao Paulo, UNIFESP, Brazil. Electronic address: araujo.ronaldo@unifesp.br.
authors.forename: R C
authors.initials: RC
authors.lastname: Araujo
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001833:Body Temperature Regulation; D001835:Body Weight; D059305:Diet, High-Fat; D004435:Eating; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D020738:Leptin / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000378:metabolism*; D013156:Splenectomy; D014280:Triglycerides / Q000378:metabolism
chemical_list: D007328:Insulin; D020738:Leptin; D014280:Triglycerides; D005947:Glucose
keywords: Leptin; Splenectomy; Temperature; Triglyceridemia