title: Beta-chitosan extracted from Loligo Japonica for a potential use to inhibit Newcastle disease.
abstract: Beta-chitosan has a parallel structure, which differs from alpha-chitosan's antiparallel structure while producing different properties and difficulties. In this paper, we prepared the beta-chitosan through acid and alkali methods and the resultant material was characterized by elemental analysis, FT-IR, HPLC, XRD, NMR and AFS. To increase the solubility and biological activity of the beta-chitosan, we degraded it through microwave-assisted process. After characterization, we determined that the chitosan had not changed its configuration during the reaction with H2O2 under microwave irradiation. The inhibitory activity of the degraded chitosan for Newcastle disease was revealed by a hemagglutination test and RT-PCR. The yield of the beta-chitosan was approximately 30%, and its molecular weight can be degraded to 1000 to 10,000g/mol. Moreover, the degraded β-chitosan has higher antiviral activity, reducing the hemagglutination titre to zero, compared with alpha-chitosan. Therefore, beta-chitosan has good development prospects during the development of veterinary drugs for Newcastle disease.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of the Chinese Academy of Sciences, Beijing 100049, China. Electronic address: hexiaofei12@mails.ucas.ac.cn.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: xingronge@qdio.ac.cn.
authors.forename: Ronge
authors.initials: R
authors.lastname: Xing
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: likecheng777@163.com.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: ykqin@qdio.ac.cn.
authors.forename: Yukun
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of the Chinese Academy of Sciences, Beijing 100049, China. Electronic address: zouping246@sina.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: sliu@qdio.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: huahuayu@qdio.ac.cn.
authors.forename: Huahua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: pcli@qdio.ac.cn.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology*; D002642:Chick Embryo; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D049834:Loligo / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009521:Newcastle Disease / Q000188:drug therapy / Q000821:virology; D009522:Newcastle disease virus / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000998:Antiviral Agents; D048271:Chitosan
keywords: Antiviral activities for Newcastle disease; Degraded beta-chitosan; Microwave-assisted