title: Exposure of native bees foraging in an agricultural landscape to current-use pesticides.
abstract: The awareness of insects as pollinators and indicators of environmental quality has grown in recent years, partially in response to declines in honey bee (Apis mellifera) populations. While most pesticide research has focused on honey bees, there has been less work on native bee populations. To determine the exposure of native bees to pesticides, bees were collected from an existing research area in northeastern Colorado in both grasslands (2013-2014) and wheat fields (2014). Traps were deployed bi-monthly during the summer at each land cover type and all bees, regardless of species, were composited as whole samples and analyzed for 136 current-use pesticides and degradates. This reconnaissance approach provides a sampling of all species and represents overall pesticide exposure (internal and external). Nineteen pesticides and degradates were detected in 54 composite samples collected. Compounds detected in >2% of the samples included: insecticides thiamethoxam (46%), bifenthrin (28%), clothianidin (24%), chlorpyrifos (17%), imidacloprid (13%), fipronil desulfinyl (7%; degradate); fungicides azoxystrobin (17%), pyraclostrobin (11%), fluxapyroxad (9%), and propiconazole (9%); herbicides atrazine (19%) and metolachlor (9%). Concentrations ranged from 1 to 310 ng/g for individual pesticides. Pesticides were detected in samples collected from both grasslands and wheat fields; the location of the sample and the surrounding land cover at the 1000 m radius influenced the pesticides detected but because of a small number of temporally comparable samples, correlations between pesticide concentration and land cover were not significant. The results show native bees collected in an agricultural landscape are exposed to multiple pesticides, these results can direct future research on routes/timing of pesticide exposure and the design of future conservation efforts for pollinators.
authors.affiliation: U.S. Geological Survey, California Water Science Center, 6000 J Street, Placer Hall, Sacramento, CA 95819, USA. Electronic address: mhladik@usgs.gov.
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Hladik
authors.affiliation: U.S. Geological Survey, Fort Collins Science Center, 2150 Centre Ave Bldg C, Fort Collins, CO 80526, USA,. Electronic address: vandeverm@usgs.gov.
authors.forename: Mark
authors.initials: M
authors.lastname: Vandever
authors.affiliation: U.S. Geological Survey, New Jersey Water Science Center, 3450 Princeton Pike, Suite 110, Lawrenceville, NJ 08648, USA. Electronic address: ksmall@usgs.gov.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Smalling
mesh_terms: D000383:Agriculture; D000818:Animals; D001516:Bees / Q000502:physiology*; D003120:Colorado; D004781:Environmental Exposure / Q000032:analysis* / Q000706:statistics & numerical data; D004785:Environmental Pollutants / Q000032:analysis*; D004787:Environmental Pollution; D010575:Pesticides / Q000032:analysis*; D054817:Pollination
chemical_list: D004785:Environmental Pollutants; D010575:Pesticides
keywords: Agroecosystems; Crop production; Native bees; Pesticides