title: Glucaric acids from Leonurus japonicus.
abstract: Three new glucaric acids, namely 2-feruloyl-4-syringoyl or 5-feruloyl-3-syringoyl glucaric acid (1), 2-syringoyl-4-feruloyl or 5-syringoyl-3-feruloyl glucaric acid (2), and 3-feruloyl-4-syringoyl or 4-feruloyl-3-syringoyl glucaric acid (3), were isolated from Leonurus japonicus Houtt. Their structures were elucidated by detailed spectroscopic means including UV, IR, HR-ESI-MS, 1D and 2D NMR data spectra. The bioactive assays of compounds 1-3 against hepatoprotection activity were determined. The result suggested that compound 2 exhibited a moderate hepatoprotection activity and the cell survival rate was 74% (10(-5)mol/L), using bicyclol (survival rate: 66%, 10(-5)mol/L) as a positive control. Furthermore, compounds 1-3 were evaluated cytotoxic activities in vitro using HCT-8, Bel-7402, BGC-823, A-549, and A2780 model and the results exhibited no obvious cytotoxicity activity.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, People's Republic of China.
authors.forename: Jianshuang
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Pharmacy, The Second Affiliated Hospital of Nanchang, Nanchang 330000, People's Republic of China.
authors.forename: Yixiu
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, People's Republic of China.
authors.forename: Ziming
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, People's Republic of China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, People's Republic of China. Electronic address: pczhang@imm.ac.cn.
authors.forename: Peicheng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D005937:Glucaric Acid / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D031332:Leonurus / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D005937:Glucaric Acid
keywords: Cytotoxicity activities; Glucaric acid; Hepatoprotection activity; Leonurus japonicus Houtt