title: Transcriptional Profiling of Candida albicans in the Host.
abstract: Gene expression profiling has become an important tool for determining gene functions, performing phenotypic analysis, and quantifying the differential expression of individual transcripts under various conditions. Candida albicans gene expression is highly responsive to environmental conditions and rapidly adapts to various niches within the host. Here, we describe a mouse model of gastrointestinal colonization with C. albicans, the measurement of colonization in fecal pellets, and the collection of samples for transcriptional profiling. We describe how to extract and purify RNA suitable for analysis via RT-qPCR or microarray.
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA, 02111, USA.
authors.forename: Kearney T W
authors.initials: KT
authors.lastname: Gunsalus
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA, 02111, USA. carol.kumamoto@tufts.edu.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Kumamoto
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology; D005260:Female; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction
keywords: Candida albicans; Gene expression; Host; RNA; Transcriptional profiling