title: The Effect of Interferon-γ and Zoledronate Treatment on Alpha-Tricalcium Phosphate/Collagen Sponge-Mediated Bone-Tissue Engineering.
abstract: Inflammatory responses are frequently associated with the expression of inflammatory cytokines and severe osteoclastogenesis, which significantly affect the efficacy of biomaterials. Recent findings have suggested that interferon (IFN)-γ and zoledronate (Zol) are effective inhibitors of osteoclastogenesis. However, little is known regarding the utility of IFN-γ and Zol in bone tissue engineering. In this study, we generated rat models by generating critically sized defects in calvarias implanted with an alpha-tricalcium phosphate/collagen sponge (α-TCP/CS). At four weeks post-implantation, the rats were divided into IFN-γ, Zol, and control (no treatment) groups. Compared with the control group, the IFN-γ and Zol groups showed remarkable attenuation of severe osteoclastogenesis, leading to a significant enhancement in bone mass. Histomorphometric data and mRNA expression patterns in IFN-γ and Zol-injected rats reflected high bone-turnover with increased bone formation, a reduction in osteoclast numbers, and tumor necrosis factor-α expression. Our results demonstrated that the administration of IFN-γ and Zol enhanced bone regeneration of α-TCP/CS implants by enhancing bone formation, while hampering excess bone resorption.
authors.affiliation: Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan. li-peiqi@cc.osaka-dent.ac.jp.
authors.forename: Peiqi
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Biomaterials, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan. honda-y@cc.osaka-dent.ac.jp.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Hashimoto
authors.affiliation: Institute of Dental Research, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan. honda-y@cc.osaka-dent.ac.jp.
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Honda
authors.affiliation: Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan. arima.dental@gmail.com.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Arima
authors.affiliation: Department of Orthodontics, Graduate School of Dentistry, Osaka Dental University, 8-1 Kuzuha Hanazonocho, Hirakata, Osaka 573-1121, Japan. naoyuki@cc.osaka-dent.ac.jp.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D004164:Diphosphonates / Q000494:pharmacology*; D048091:Guided Tissue Regeneration / Q000379:methods*; D007093:Imidazoles / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D016348:Osseointegration / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D000077211:Zoledronic Acid
chemical_list: D001672:Biocompatible Materials; D050071:Bone Density Conservation Agents; D002130:Calcium Phosphates; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D007371:Interferon-gamma; D003094:Collagen; C018392:tricalcium phosphate
keywords: RANKL; TNF-α; bone tissue engineering; inflammatory response; interferon-γ; osteoclastogenesis; zoledronate; α-TCP/CS