title: Real-time tracking of hydrogen peroxide secreted by live cells using MnO2 nanoparticles intercalated layered doubled hydroxide nanohybrids.
abstract: We report a facile and green method for the fabrication of new type of electrocatalysts based on MnO2 nanoparticles incorporated on MgAl LDH P-type semiconductive channel and explore its practical applications as high-performance electrode materials for electrochemical biosensor. A series of MgAl layered doubled hydroxide (LDH) nanohybrids with fixed Mg/Al (M(2+)/M(3+) atomic ratio of 3) and varied amount of MnCl2.4H2O are fabricated by a facile co-precipitation method. This approach demonstrates the combination of distinct properties including excellent intercalation features of LDH for entrapping nanoparticles and high loading of MnO2 nanoparticles in the host layers of LDH. Among all samples, Mn5-MgAl with 0.04% loaded manganese has a good crystalline morphology. A well-dispersed MnO2 nanoparticles encapsulated into the host matrix of hydrotalcite exhibit enhanced electrocatalytic activity towards the reduction of H2O2 as well as excellent stability, selectivity and reproducibility due to synergistic effect of good catalytic ability of MnO2 and conductive MgAl LDH. Glass carbon electrode (GCE) modified with Mn5-MgAl possesses a wide linear range of 0.05-78 mM, lowest detection limit 5 μM (S/N = 3) and detection sensitivity of 0.9352 μAmM(-1). This outstanding performance enables it to be used for real-time tracking of H2O2 secreted by live HeLa cells. This work may provide new insight in clinical diagnosis, on-site environmental analysis and point of care testing devices.
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asif
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Aziz
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China; Hue Industrial College, 70 Nguyen Hue, Hue, Thua Thien Hue, 531081, Viet Nam.
authors.forename: Anh Quang
authors.initials: AQ
authors.lastname: Dao
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Abdul
authors.initials: A
authors.lastname: Hakeem
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Dong
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China.
authors.forename: Guoan
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China; Shenzhen Institute of Huazhong University of Science & Technology, Shenzhen, 518000, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, 430074, PR China; Shenzhen Institute of Huazhong University of Science & Technology, Shenzhen, 518000, PR China. Electronic address: liuhf@hust.edu.cn.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Liu
mesh_terms: D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010087:Oxides / Q000737:chemistry*
chemical_list: D017895:Manganese Compounds; D010087:Oxides; D006861:Hydrogen Peroxide; C016552:manganese dioxide
keywords: Electrochemical biosensor; Hydrogen peroxide; Layered doubled hydroxide nanohybrids; Live cells; Manganese dioxide nanoparticles