title: A Reversible DNA Logic Gate Platform Operated by One- and Two-Photon Excitations.
abstract: We demonstrate the use of two different wavelength ranges of excitation light as inputs to remotely trigger the responses of the self-assembled DNA devices (D-OR). As an important feature of this device, the dependence of the readout fluorescent signals on the two external inputs, UV excitation for 1 min and/or near infrared irradiation (NIR) at 800 nm fs laser pulses, can mimic function of signal communication in OR logic gates. Their operations could be reset easily to its initial state. Furthermore, these DNA devices exhibit efficient cellular uptake, low cytotoxicity, and high bio-stability in different cell lines. They are considered as the first example of a photo-responsive DNA logic gate system, as well as a biocompatible, multi-wavelength excited system in response to UV and NIR. This is an important step to explore the concept of photo-responsive DNA-based systems as versatile tools in DNA computing, display devices, optical communication, and biology.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China).
authors.forename: Dick Yan
authors.initials: DY
authors.lastname: Tam
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China).
authors.forename: Ziwen
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China).
authors.forename: Miu Shan
authors.initials: MS
authors.lastname: Chan
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China).
authors.forename: Ling Sum
authors.initials: LS
authors.lastname: Liu
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China).
authors.forename: Man Ching
authors.initials: MC
authors.lastname: Cheung
authors.affiliation: Laboratoire de Conception et Application des Molécules bioactives, UMR Université of Strasbourg-CNRS 7199, Faculté de Pharmacie, Université de Strasbourg (France).
authors.forename: Frederic
authors.initials: F
authors.lastname: Bolze
authors.affiliation: Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China).
authors.forename: Chung
authors.initials: C
authors.lastname: Tin
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong SAR (China). peggylo@cityu.edu.hk.|Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057 (China). peggylo@cityu.edu.hk.
authors.forename: Pik Kwan
authors.initials: PK
authors.lastname: Lo
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D005453:Fluorescence; D008128:Logic; D017785:Photons
chemical_list: D004247:DNA
keywords: logic gates; optical communication; photo-cleavage; photo-responsive; reversible