title: Expression of HIP/PAP in hepatocellular carcinoma and effect of siRNA on migration and invasion in HCC cells.
abstract: OBJECTIVE
  To investigate the expression of HIP/PAP in hepatocellular carcinoma (HCC) patients and explore its role in migration and invasion of HCC.
  
  
  METHODS
  The expression of HIP/PAP in HCC tissue and corresponding adjacent noncancerous tissue was assessed by IHC, RT-PCR and Western blot. The correlation between clinicopathological features and HIP/PAP expression was analyzed. The role of HIP/PAP on invasion and migration of HCC cells was observed by RNA interference, wound healing and Transwell assay.
  
  
  RESULTS
  Both mRNA and protein expression of HIP/PAP was upregulated in HCC tissues compared to tumor-adjacent tissue and correlated with poor tumor differentiation, advanced tumor stage and vascular invasion. HIP/PAP expression was also upregulated in HCC cells, and silencing its expression by specific siRNA could inhibit the invasion and migration of HCC cells.
  
  
  CONCLUSIONS
  HIP/APA is overexpressed in HCC and contributes to the migration and invasion of HCC cells.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China. Electronic address: lidechun2133@163.com.
authors.forename: De-Chun
authors.initials: DC
authors.lastname: Li
keywords: HIP/PAP; Hepatocellular carcinoma; Invasion; Migration; Prognosis