title: Insights into immune responses in oral cancer through proteomic analysis of saliva and salivary extracellular vesicles.
abstract: The development and progression of oral cavity squamous cell carcinoma (OSCC) involves complex cellular mechanisms that contribute to the low five-year survival rate of approximately 20% among diagnosed patients. However, the biological processes essential to tumor progression are not completely understood. Therefore, detecting alterations in the salivary proteome may assist in elucidating the cellular mechanisms modulated in OSCC and improve the clinical prognosis of the disease. The proteome of whole saliva and salivary extracellular vesicles (EVs) from patients with OSCC and healthy individuals were analyzed by LC-MS/MS and label-free protein quantification. Proteome data analysis was performed using statistical, machine learning and feature selection methods with additional functional annotation. Biological processes related to immune responses, peptidase inhibitor activity, iron coordination and protease binding were overrepresented in the group of differentially expressed proteins. Proteins related to the inflammatory system, transport of metals and cellular growth and proliferation were identified in the proteome of salivary EVs. The proteomics data were robust and could classify OSCC with 90% accuracy. The saliva proteome analysis revealed that immune processes are related to the presence of OSCC and indicate that proteomics data can contribute to determining OSCC prognosis.
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Flavia V
authors.initials: FV
authors.lastname: Winck
authors.affiliation: Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, ICESP, São Paulo, SP, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Prado Ribeiro
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Romênia
authors.initials: R
authors.lastname: Ramos Domingues
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol, CTBE, CNPEM, Campinas, SP, Brazil.
authors.forename: Liu Yi
authors.initials: LY
authors.lastname: Ling
authors.affiliation: Laboratório Nacional de Ciência e Tecnologia do Bioetanol, CTBE, CNPEM, Campinas, SP, Brazil.
authors.forename: Diego Mauricio
authors.initials: DM
authors.lastname: Riaño-Pachón
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.|Departamento de Ciencias Básicas Biomédicas, Universidad de Talca (UTALCA), Talca, Chile.
authors.forename: César
authors.initials: C
authors.lastname: Rivera
authors.affiliation: Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, ICESP, São Paulo, SP, Brazil.
authors.forename: Thaís Bianca
authors.initials: TB
authors.lastname: Brandão
authors.affiliation: Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, ICESP, São Paulo, SP, Brazil.
authors.forename: Adriele Ferreira
authors.initials: AF
authors.lastname: Gouvea
authors.affiliation: Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Alan Roger
authors.initials: AR
authors.lastname: Santos-Silva
authors.affiliation: Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, UNICAMP, Piracicaba, SP, Brazil.
authors.forename: Ricardo D
authors.initials: RD
authors.lastname: Coletta
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, SP, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000276:immunology / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D000067128:Extracellular Vesicles / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology* / Q000378:metabolism*; D011379:Prognosis; D020543:Proteome / Q000276:immunology* / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012463:Saliva / Q000276:immunology / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome