title: Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine.
abstract: In the present work the effect of cyclodextrin and coronatine on both trans-resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans-resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans-resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans-resveratrol in the spent medium was not detected. Therefore, despite the fact that trans-resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans-R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain. Electronic address: lorena.almagro@um.es.
authors.forename: Lorena
authors.initials: L
authors.lastname: Almagro
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Sarai
authors.initials: S
authors.lastname: Belchí-Navarro
authors.affiliation: Department of Agrochemistry and Biochemistry, Faculty of Sciences, University of Alicante, Alicante, Spain.
authors.forename: Ascensión
authors.initials: A
authors.lastname: Martínez-Márquez
authors.affiliation: Department of Agrochemistry and Biochemistry, Faculty of Sciences, University of Alicante, Alicante, Spain.
authors.forename: Roque
authors.initials: R
authors.lastname: Bru
authors.affiliation: Department of Plant Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: María A
authors.initials: MA
authors.lastname: Pedreño
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003505:Cyclodextrins / Q000494:pharmacology*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D007192:Indenes / Q000494:pharmacology*; D042541:Intracellular Space / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D013535:Suspensions; D027843:Vitis / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003505:Cyclodextrins; D007192:Indenes; D013267:Stilbenes; D013535:Suspensions; C058487:coronatine; D000077185:Resveratrol
keywords: Coronatine; Cyclodextrins; Gene expression; Grapevine suspension-cultured cells; trans-resveratrol