title: Pharmacokinetic study of dendrobine in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry.
abstract: Dendrobine, considered as the major active alkaloid compound, has been used for the quality control and discrimination of Dendrobium which is documented in the Chinese Pharmacopoeia. In this work, a sensitive and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for determination of dendrobine in rat plasma is developed. After addition of caulophyline as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 (2.1 ×100 mm, 1.7 µm) column with acetonitrile and 0.1% formic acid as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 264.2 → 70.0 for dendrobine and m/z 205.1 → 58.0 for IS. Calibration plots were linear throughout the range 2-1000 ng/mL for dendrobine in rat plasma. The RSDs of intra-day and inter-day precision were both <13%. The accuracy of the method was between 95.4 and 103.9%. The method was successfully applied to pharmacokinetic study of dendrobine after intravenous administration. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Wenzhou Medical University, Lishui, 323000, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, the Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, 325000, China.
authors.forename: Haiya
authors.initials: H
authors.lastname: Wu
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Wenzhou Medical University, Lishui, 323000, China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Geng
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Lin
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Zezheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Ma
authors.affiliation: The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Wenzhou Medical University, Lishui, 323000, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Laboratory Animal Center, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Wen
mesh_terms: D000470:Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; C002077:dendrobine
keywords: UPLC-MS/MS; dendrobine; pharmacokinetics; rat plasma