title: Microsecond-pulsed dielectric barrier discharge plasma stimulation of tissue macrophages for treatment of peripheral vascular disease.
abstract: Angiogenesis is the formation of new blood vessels from pre-existing vessels and normally occurs during the process of inflammatory reactions, wound healing, tissue repair, and restoration of blood flow after injury or insult. Stimulation of angiogenesis is a promising and an important step in the treatment of peripheral artery disease. Reactive oxygen species have been shown to be involved in stimulation of this process. For this reason, we have developed and validated a non-equilibrium atmospheric temperature and pressure short-pulsed dielectric barrier discharge plasma system, which can non-destructively generate reactive oxygen species and other active species at the surface of the tissue being treated. We show that this plasma treatment stimulates the production of vascular endothelial growth factor, matrix metalloproteinase-9, and CXCL 1 that in turn induces angiogenesis in mouse aortic ringsin vitro. This effect may be mediated by the direct effect of plasma generated reactive oxygen species on tissue.
authors.affiliation: AJ Drexel Plasma Institute, Drexel University , Camden, New Jersey 08103, USA.
authors.forename: V
authors.initials: V
authors.lastname: Miller
authors.affiliation: AJ Drexel Plasma Institute, Drexel University , Camden, New Jersey 08103, USA.
authors.forename: A
authors.initials: A
authors.lastname: Lin
authors.affiliation: Department of Physiology, Independence Blue Cross Cardiovascular Research Center, Temple University School of Medicine , Philadelphia, Pennsylvania 19140, USA.
authors.forename: F
authors.initials: F
authors.lastname: Kako
authors.affiliation: Department of Physiology, Independence Blue Cross Cardiovascular Research Center, Temple University School of Medicine , Philadelphia, Pennsylvania 19140, USA.
authors.forename: K
authors.initials: K
authors.lastname: Gabunia
authors.affiliation: Department of Physiology, Independence Blue Cross Cardiovascular Research Center, Temple University School of Medicine , Philadelphia, Pennsylvania 19140, USA.
authors.forename: S
authors.initials: S
authors.lastname: Kelemen
authors.affiliation: AJ Drexel Plasma Institute, Drexel University , Camden, New Jersey 08103, USA.
authors.forename: J
authors.initials: J
authors.lastname: Brettschneider
authors.affiliation: AJ Drexel Plasma Institute, Drexel University , Camden, New Jersey 08103, USA.
authors.forename: G
authors.initials: G
authors.lastname: Fridman
authors.affiliation: AJ Drexel Plasma Institute, Drexel University , Camden, New Jersey 08103, USA.
authors.forename: A
authors.initials: A
authors.lastname: Fridman
authors.affiliation: Department of Physiology, Independence Blue Cross Cardiovascular Research Center, Temple University School of Medicine , Philadelphia, Pennsylvania 19140, USA.
authors.forename: M
authors.initials: M
authors.lastname: Autieri