title: Note: Small anaerobic chamber for optical spectroscopy.
abstract: The study of oxygen-sensitive biological samples requires an effective control of the atmosphere in which they are housed. In this aim however, no commercial anaerobic chamber is adequate to solely enclose the sample and small enough to fit in a compact spectroscopic system with which analysis can be performed. Furthermore, spectroscopic analysis requires the probe beam to pass through the whole chamber, introducing a requirement for adequate windows. In response to these challenges, we present a 1 l anaerobic chamber that is suitable for broad-band spectroscopic analysis. This chamber has the advantage of (1) providing access, via a septum, to the sample and (2) allows the sample position to be adjusted while keeping the chamber fixed and hermetic during the experiment.
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide, ISIC, Faculté des Sciences de Base, Station 6, 1015 Lausanne, Switzerland.
authors.forename: Adrien A P
authors.initials: AA
authors.lastname: Chauvet
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Rachna
authors.initials: R
authors.lastname: Agarwal
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Cramer
authors.affiliation: Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide, ISIC, Faculté des Sciences de Base, Station 6, 1015 Lausanne, Switzerland.
authors.forename: Majed
authors.initials: M
authors.lastname: Chergui
mesh_terms: D004867:Equipment Design; D006418:Heme / Q000737:chemistry; D044085:Microfluidics / Q000295:instrumentation; D010084:Oxidation-Reduction; D010100:Oxygen / Q000008:administration & dosage / Q000737:chemistry; D013057:Spectrum Analysis / Q000295:instrumentation* / Q000379:methods
chemical_list: D006418:Heme; D010100:Oxygen