title: Activation of MAPK Is Required for ROS Generation and Exocytosis in HMC-1 Cells Induced by Trichomonas vaginalis-Derived Secretory Products.
abstract: Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47(phox) in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.|Department of Basic Science, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
authors.forename: Giimaa
authors.initials: G
authors.lastname: Narantsogt
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Arim
authors.initials: A
authors.lastname: Min
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Nam
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Lee
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Kyeong Ah
authors.initials: KA
authors.lastname: Kim
authors.affiliation: Department of Basic Science, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
authors.forename: Gurbadam
authors.initials: G
authors.lastname: Agvaandaram
authors.affiliation: Department of Basic Science, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
authors.forename: Temuulen
authors.initials: T
authors.lastname: Dorjsuren
authors.affiliation: DR-CNRS Centre de Recherche Biomedical, INSERM U773, Universite Paris & Denis Diderot, Paris, France.
authors.forename: Jamel
authors.initials: J
authors.lastname: El-Benna
authors.affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Myeong Heon
authors.initials: MH
authors.lastname: Shin
mesh_terms: D015550:Cell Degranulation; D002460:Cell Line; D005089:Exocytosis; D006801:Humans; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014246:Trichomonas vaginalis / Q000378:metabolism*; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D037521:Virulence Factors; D020928:Mitogen-Activated Protein Kinases
keywords: MAPK; ROS generation; Trichomonas vaginalis; exocytotic degranulation; mast cell; secretory product