title: Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae.
abstract: In dry savannahs of West-Africa, the malarial mosquitoes of the Anopheles gambiae sensu stricto complex annually survive the harsh desiccating conditions of the dry season. However, the physiological and biochemical mechanisms underlying how these mosquitoes survive such desiccating conditions are still undefined, and controversial. In this context, we provide the first work examining both proteomic and metabolomic changes in the two molecular forms of A. gambiae s.s (M and S forms) experimentally exposed to the rainy and dry season conditions as they experience in the field. Protein abundances of the mosquitoes were measured using a two-dimensional fluorescence difference gel electrophoresis (2D DIGE) coupled with a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) and tandem mass spectrometry (MS) for protein identification. These assays were conducted by Applied Biomics (http://www.appliedbiomics.com, Applied Biomics, Inc. Hayward, CA, USA), and the mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD000294. The metabolomic analysis was conducted using both Acquity UPLC(®) system (for amino acid identification), and a gas-chromatography-mass spectrometry platform (for sugars identification). Metabolomic fingerprintings were assessed in the University of Rennes 1, UMR CNRS 6553 EcoBio (France). A detailed interpretation of the obtained data can be found in Hidalgo et al. (2014) [1] (Journal of Insect Physiology (2014)).
authors.affiliation: Université de Rennes 1, UMR CNRS 6553 Ecobio, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205 35042 Rennes Cedex, France ; Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
authors.forename: K
authors.initials: K
authors.lastname: Hidalgo
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France ; Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté 01, BP 545 Bobo-Dioulasso, Burkina Faso.
authors.forename: K
authors.initials: K
authors.lastname: Mouline
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France ; Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté 01, BP 545 Bobo-Dioulasso, Burkina Faso.
authors.forename: W
authors.initials: W
authors.lastname: Mamai
authors.affiliation: Université Claude Bernard Lyon 1, UMR CNRS 5023 LEHNA, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: N
authors.initials: N
authors.lastname: Foucreau
authors.affiliation: Institut de Recherche en Sciences de la Santé (IRSS), Direction Régionale de l'Ouest (DRO), 399 Avenue de la Liberté 01, BP 545 Bobo-Dioulasso, Burkina Faso.
authors.forename: K R
authors.initials: KR
authors.lastname: Dabiré
authors.affiliation: Université de Rennes 1, UMR INRA IGEPP, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205, 35042 Rennes Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Bouchereau
authors.affiliation: Institut de Recherche pour le Développement (IRD), UMR IRD 224-CNRS 5290-Université de Montpellier MIVEGEC, 911 Avenue Agropolis, BP 64501, 34394 Montpellier cedex 5, France.
authors.forename: F
authors.initials: F
authors.lastname: Simard
authors.affiliation: Université de Rennes 1, UMR CNRS 6553 Ecobio, Campus de Beaulieu, 263 Avenue du Général Leclerc, CS 74205 35042 Rennes Cedex, France.
authors.forename: D
authors.initials: D
authors.lastname: Renault
keywords: 2D electrophoresis; Amino acid; Metabolomic; Mosquito; Proteomic; Sugar