title: Measurement of Ensemble TRPV1 Ion Channel Currents Using Droplet Bilayers.
abstract: Electrophysiological characterization of ion channels is useful for elucidation of channel function as well as quantitative assessment of pharmaceutical effects on ion channel conductance. We used droplet bilayers to measure ensemble ion channel currents from membrane preparations made from TRPV1-expressing HEK cells. Conductance measurements showed rectification, activation by acid and capsaicin, and inhibition by capsazepine, SB 452533, and JNJ 17293212. We also quantitatively measured concentration-dependent inhibition of channel conductance through determination of capsazepine IC50 in agreement with previously published studies using patch clamp. These results, combined with the reduced apparatus and material requirements of droplet bilayers, indicate that this platform could be used for study of other physiologically relevant ion channels.
authors.affiliation: Department of Bioengineering, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Viksita
authors.initials: V
authors.lastname: Vijayvergiya
authors.affiliation: Department of Bioengineering, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Shiv
authors.initials: S
authors.lastname: Acharya
authors.affiliation: Department of Bioengineering, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Sidney P
authors.initials: SP
authors.lastname: Wilson
authors.affiliation: Department of Bioengineering, University of California Los Angeles, Los Angeles, California, United States of America.
authors.forename: Jacob J
authors.initials: JJ
authors.lastname: Schmidt
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008051:Lipid Bilayers; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D008051:Lipid Bilayers; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; C071423:capsazepine; D002211:Capsaicin