title: P-glycoprotein and its inducible expression in three bivalve species after exposure to Prorocentrum lima.
abstract: P-glycoprotein (P-gp or ABCB1) belongs to the family of ATP-binding cassette (ABC) transporters responsible for multixenobiotic resistance (MXR) in aquatic organisms. To provide more information of P-gp in shellfish, in this study, complete cDNA of P-gp in three bivalve species including Ruditapes philippinarum, Scapharca subcrenata and Tegillarca granosa were cloned and its expressions in gill, digestive gland, adductor muscle and mantle of the three bivalves were detected after exposure to Prorocentrum lima, a toxogenic dinoflagellate. The complete sequences of R. philippinarum, S. subcrenata and T. granosa P-gp showed high homology with MDR/P-gp/ABCB proteins from other species, having a typical sequence organization as full transporters from the ABCB family. Phylogenetic analyses revealed that the amino acid sequences of P-gp from S. subcrenata and T. granosa had a closest relationship, forming an independent branch, then grouping into the other branch with Mytilus californianus, Mytilus galloprovincialis and Crassostrea gigas. However, P-gp sequences from R. philippinarum were more similar to the homologs from the more distantly related Aplysia californica than to homologs from S. subcrenata and T. granosa, suggesting that bivalves P-gp might have different paralogs. P-glycoprotein expressed in all detected tissues but there were large differences between them. After exposure to P. lima, the expression of P-gp changed in the four tissues in varying degrees within the same species and between different species, but the changes in mRNA and protein level were not always synchronous.
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China; Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Huang
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zheng
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Hong-Ye
authors.initials: HY
authors.lastname: Li
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China.
authors.forename: Jie-Sheng
authors.initials: JS
authors.lastname: Liu
authors.affiliation: College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510632, China. Electronic address: tywd@jnu.edu.cn.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000469:parasitology; D018076:DNA, Complementary / Q000235:genetics; D004141:Dinoflagellida / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects; D008387:Marine Toxins / Q000633:toxicity*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018076:DNA, Complementary; D008387:Marine Toxins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical
keywords: P-glycoprotein; Prorocentrum lima; Ruditapes philippinarum; Scapharca subcrenata; Tegillarca granosa