title: An Anti-Influenza Virus Antibody Inhibits Viral Infection by Reducing Nucleus Entry of Influenza Nucleoprotein.
abstract: To date, four main mechanisms mediating inhibition of influenza infection by anti-hemagglutinin antibodies have been reported. Anti-globular-head-domain antibodies block either influenza virus receptor binding to the host cell or progeny virion release from the host cell. Anti-stem region antibodies hinder the membrane fusion process or induce antibody-dependent cytotoxicity to infected cells. In this study we identified a human monoclonal IgG1 antibody (CT302), which does not inhibit both the receptor binding and the membrane fusion process but efficiently reduced the nucleus entry of viral nucleoprotein suggesting a novel inhibition mechanism of viral infection by antibody. This antibody binds to the subtype-H3 hemagglutinin globular head domain of group-2 influenza viruses circulating throughout the population between 1997 and 2007.
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Aerin
authors.initials: A
authors.lastname: Yoon
authors.affiliation: Biotechnology Research Institute, Celltrion Inc., Incheon, South Korea.
authors.forename: Kye Sook
authors.initials: KS
authors.lastname: Yi
authors.affiliation: Institut Pasteur Korea, Gyeonggi-do, South Korea.
authors.forename: So Young
authors.initials: SY
authors.lastname: Chang
authors.affiliation: Biotechnology Research Institute, Celltrion Inc., Incheon, South Korea.
authors.forename: Sung Hwan
authors.initials: SH
authors.lastname: Kim
authors.affiliation: International Vaccine Institute, Seoul, South Korea.
authors.forename: Manki
authors.initials: M
authors.lastname: Song
authors.affiliation: International Vaccine Institute, Seoul, South Korea.
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Choi
authors.affiliation: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
authors.forename: Melissa
authors.initials: M
authors.lastname: Bourgeois
authors.affiliation: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
authors.forename: M Jaber
authors.initials: MJ
authors.lastname: Hossain
authors.affiliation: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Chen
authors.affiliation: Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
authors.forename: Ruben O
authors.initials: RO
authors.lastname: Donis
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Hyori
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Yujean
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Do Been
authors.initials: DB
authors.lastname: Hwang
authors.affiliation: Institut Pasteur Korea, Gyeonggi-do, South Korea.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Min
authors.affiliation: Biotechnology Research Institute, Celltrion Inc., Incheon, South Korea.
authors.forename: Shin Jae
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Junho
authors.initials: J
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D002460:Cell Line; D004195:Disease Models, Animal; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005289:Ferrets; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology*; D008297:Male; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009698:Nucleoproteins / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000821:virology; D011485:Protein Binding; D011487:Protein Conformation; D016415:Sequence Alignment; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007074:Immunoglobulin G; D009698:Nucleoproteins