title: Human microRNAs profiling in response to influenza A viruses (subtypes pH1N1, H3N2, and H5N1).
abstract: MicroRNAs (miRNAs) play an important role in regulation of gene silencing and are involved in many cellular processes including inhibition of infected viral replication. This study investigated cellular miRNA expression profiles operating in response to influenza virus in early stage of infection which might be useful for understanding and control of viral infection. A549 cells were infected with different subtypes of influenza virus (pH1N1, H3N2 and H5N1). After 24 h post-infection, miRNAs were extracted and then used for DNA library construction. All DNA libraries with different indexes were pooled together with equal concentration, followed by high-throughput sequencing based on MiSeq platform. The miRNAs were identified and counted from sequencing data by using MiSeq reporter software. The miRNAs expressions were classified into up and downregulated miRNAs compared to those found in non-infected cells. Mostly, each subtype of influenza A virus triggered the upregulated responses in miRNA expression profiles. Hsa-miR-101, hsa-miR-193b, hsa-miR-23b, and hsa-miR-30e* were upregulated when infected with all three subtypes of influenza A virus. Target prediction results showed that virus infection can trigger genes in cellular process, metabolic process, developmental process and biological regulation. This study provided some insights into the cellular miRNA profiling in response to various subtypes of influenza A viruses in circulation and which have caused outbreaks in human population. The regulated miRNAs might be involved in virus-host interaction or host defense mechanism, which should be investigated for effective antiviral therapeutic interventions.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Jarika
authors.initials: J
authors.lastname: Makkoch
authors.affiliation: Research affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Witthaya
authors.initials: W
authors.lastname: Poomipak
authors.affiliation: Joint Chulalongkorn University - University of Liverpool PhD Programme in Biomedical Sciences and Biotechnology, Bangkok 10330, Thailand.
authors.forename: Suthat
authors.initials: S
authors.lastname: Saengchoowong
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Kritsada
authors.initials: K
authors.lastname: Khongnomnan
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand.
authors.forename: Kesmanee
authors.initials: K
authors.lastname: Praianantathavorn
authors.affiliation: Systems Biology Center, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330 Thailand.
authors.forename: Thananya
authors.initials: T
authors.lastname: Jinato
authors.affiliation: Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand Systems Biology Center, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330 Thailand sp.medbiochemcu@gmail.com.
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
mesh_terms: D002460:Cell Line; D019295:Computational Biology; D004847:Epithelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000254:growth & development*; D053122:Influenza A Virus, H3N2 Subtype / Q000254:growth & development*; D053124:Influenza A Virus, H5N1 Subtype / Q000254:growth & development*; D035683:MicroRNAs / Q000032:analysis*; D017422:Sequence Analysis, DNA
chemical_list: D035683:MicroRNAs
keywords: expression profiles; influenza A virus; miRNAs; next-generation sequencing