title: Import of Fluorescent RNA into Mitochondria of Living Cells.
abstract: Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences.
authors.affiliation: Department No. 75, Membrane Transprot Biophysics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1084, Prague 4, 14220, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Zelenka
authors.affiliation: Department No. 75, Membrane Transprot Biophysics, Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1084, Prague 4, 14220, Czech Republic. jezek@biomed.cas.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Ježek
mesh_terms: D045744:Cell Line, Tumor; D004272:DNA, Mitochondrial / Q000235:genetics; D005456:Fluorescent Dyes; D056945:Hep G2 Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D034443:RNA Transport / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D014162:Transfection / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial; D005456:Fluorescent Dyes; D000077278:RNA, Mitochondrial; D012341:RNA, Ribosomal, 5S; D012313:RNA
keywords: 5S-rRNA; Fluorescent in vivo hybridization of mtDNA; Mitochondrial DNA; Mitochondrial nucleoids; Nucleic acid import into mitochondria