title: A human monoclonal IgE antibody that binds to MGL_1304, a major allergen in human sweat, without activation of mast cells and basophils.
abstract: MGL_1304, a major allergen in human sweat for patients with atopic dermatitis and/or cholinergic urticaria, is secreted from Malassezia globosa on human skin. The amounts of MGL_1304 and IgE against MGL_1304 are evaluated by the histamine release test using basophils or mast cells sensitized with serum containing IgE against MGL_1304, and enzyme linked sorbent assay (ELISA) using MGL_1304 and anti-MGL_1304 antibodies. Here, we identified a human monoclonal IgE (ABS-IgE) that binds to the high affinity IgE receptor (FcεRI) and MGL_1304 with high affinity (KD = 1.99 nM) but does not release histamine from basophils and mast cells. An ELISA using ABS-IgE as a standard IgE revealed that the amount of IgE against MGL_1304 (1000 U/ml) in the standard sera of patients with AD, employed in our previous report, is 32 ng/ml. A sandwich ELISA using ABS-IgE as a detection antibody showed approximately 10 times lower detection limit for MGL_1304 than ELISA in which MGL_1304 is directly bound to an ELISA plate. Moreover, ABS-IgE prevented histamine release from mast cells and basophils by neutralizing MGL_1304 not only in a free form in solution, but also on FcεRI expressed on the cell surface without cell activation. ABS-IgE may be used both to quantify the amount of MGL_1304 and anti-MGL_1304 IgE, and possibly for the treatment of diseases caused/aggravated by type I allergy to MGL_1304.
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Hiragun
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hiragun
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kan
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kawaguchi
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Yanase
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Tanaka
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Takahagi
authors.affiliation: Department of Dermatology, Institute of Biochemical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8851, Japan. Electronic address: ed1h-w1de-road@hiroshima-u.ac.jp.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Hide
mesh_terms: D000485:Allergens / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000276:immunology / Q000382:microbiology; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008290:Malassezia / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D017455:Receptors, IgE / Q000276:immunology; D013542:Sweat / Q000276:immunology*
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal; D017455:Receptors, IgE; D007073:Immunoglobulin E
keywords: Atopic dermatitis; ELISA; Human IgE; MGL_1304; Malassezia globosa; Sweat antigen