title: Identification and Analysis of Regulatory Elements in Porcine Bone Morphogenetic Protein 15 Gene Promoter.
abstract: Bone morphogenetic protein 15 (BMP15) is secreted by the mammalian oocytes and is indispensable for ovarian follicular development, ovulation, and fertility. To determine the regulation mechanism of BMP15 gene, the regulatory sequence of porcine BMP15 was investigated in this study. The cloned BMP15 promoter retains the cell-type specificity, and is activated in cells derived from ovarian tissue. The luciferase assays in combination with a series of deletion of BMP15 promoter sequence show that the -427 to -376 bp region of BMP15 promoter is the primary regulatory element, in which there are a number of transcription factor binding sites, including LIM homeobox 8 (LHX8), newborn ovary homeobox gene (NOBOX), and paired-like homeodomain transcription factor 1 (PITX1). Determination of tissue-specific expression reveals that LHX8, but not PITX1 and NOBOX, is exclusively expressed in pig ovary tissue and is translocated into the cell nuclei. Overexpression of LHX8 in Chinese hamster ovary (CHO) cells could significantly promote BMP15 promoter activation. This study confirms a key regulatory element that is located in the proximal region of BMP15 promoter and is regulated by the LHX8 factor.
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Xi'an 712100, Shaanxi, China. wanqianhui_casey@163.com.
authors.forename: Qianhui
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Xi'an 712100, Shaanxi, China. wangyaxianwyx@163.com.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Xi'an 712100, Shaanxi, China. hhwang101@nwsuaf.edu.cn.
authors.forename: Huayan
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D060850:LIM-Homeodomain Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D051761:Paired Box Transcription Factors / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D013552:Swine; D015533:Transcriptional Activation
chemical_list: D055430:Bone Morphogenetic Protein 15; D060850:LIM-Homeodomain Proteins; D051761:Paired Box Transcription Factors; C101485:homeobox protein PITX1
keywords: BMP15; LHX8; pig; promoter; transcription factor