title: Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process.
abstract: This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffer was used rather than citrate buffer. Initially, with each process variable tests, the optimal values were 100 minutes of milling, an enzyme concentration of 16 FPU/g-biomass, and a 12-hour enzymatic hydrolysis. Typically, interactions between these experimental conditions and their effects on glucose production were next investigated using RSM. Glucose yields from the Pinus rigida waste exceeded 80% with several of the conditions tested, demonstrating that milling can be used to obtain high levels of glucose bioconversion from woody biomass for biorefinery purposes.
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea ; Department of Chemical Engineer, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Jeong Heo
authors.initials: JH
authors.lastname: Kwon
authors.affiliation: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
authors.forename: Siseon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Forest Products and Technology, Chonnam National University, Gwangju 61186, Republic of Korea.
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
authors.forename: Youn-Woo
authors.initials: YW
authors.lastname: Hong
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Chang
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
authors.forename: Daekyung
authors.initials: D
authors.lastname: Sung
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineer, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Byoung-In
authors.initials: BI
authors.lastname: Sang
authors.affiliation: School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Mitchell
authors.affiliation: Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea.
authors.forename: Jin Hyung
authors.initials: JH
authors.lastname: Lee
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000096:biosynthesis* / Q000737:chemistry; D002480:Cellulase / Q000737:chemistry*; D005947:Glucose / Q000096:biosynthesis*; D006868:Hydrolysis; D028223:Pinus / Q000737:chemistry / Q000378:metabolism; D014934:Wood / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D002480:Cellulase; D005947:Glucose