title: Enzymatic-induced upconversion photoinduced electron transfer for sensing tyrosine in human serum.
abstract: This paper reports a novel nanosensor for tyrosine based on photoinduced electron-transfer (PET) between NaYF4:Yb, Tm upconversion nanoparticles (UCNPs) and melanin-like polymers. Melanin-like films were obtained from catalytic oxidation of tyrosine by tyrosinase, and deposited on the surface of UCNPs, and then quenched the fluorescence of UCNPs. Under the optimized conditions, the fluorescence quenching of UCNPs showed a good linear response to tyrosine concentration in the range of 0.8-100 μΜ with a detection limit of 1.1 μΜ. Meanwhile, it showed good sensitivity, stability and has been successfully applied to the detection of tyrosine in human serum.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
authors.forename: Qiongqiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
authors.forename: Aijin
authors.initials: A
authors.lastname: Fang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. Electronic address: zhangyy@hunnu.edu.cn.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation*; D004579:Electron Transport / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005459:Fluorides / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D008027:Light; D014442:Monophenol Monooxygenase / Q000737:chemistry* / Q000528:radiation effects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013667:Technetium / Q000737:chemistry* / Q000528:radiation effects; D014443:Tyrosine / Q000032:analysis* / Q000528:radiation effects; D015019:Yttrium / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D004800:Enzymes, Immobilized; C525362:sodium yttriumtetrafluoride; D014443:Tyrosine; D015019:Yttrium; D013667:Technetium; D014442:Monophenol Monooxygenase; D005459:Fluorides
keywords: Enzymatic; Photoinduced electron-transfer; Tyrosine; Upconversion nanoparticles