title: Synthesis, characterization, DNA interactions, DNA cleavage, radical scavenging activity, antibacterial, anti-proliferative and docking studies of new transition metal complexes.
abstract: The compound N-(2-hydroxybenzylidene)-1-ethyl-1, 4-dihydro-7-methyl-4-oxo-1, 8 naphthyridine-3-carbohydrazide (LH) and its Cu (II), Co (II) and Zn (II) complexes were synthesized and characterized. The absorption spectral titrations and competitive DNA binding studies depicted those complexes of title compound bind to CT-DNA through intercalation. Interestingly [Cu (II)-(L2)] showed relatively high binding constant value (6.61 x 10(5) M(-1)) compared to [Co (II)-(L2)] (4.378× 10(5) M(-1)) and [Zn (II)-(L2)] (3.1x10(5) M(-1)). Ligand and its complexes were also examined for DNA nuclease activity against pBR-322 plasmid DNA, which showed that [Cu (II)-(L2)] had the best hydrolytic cleavage property displaying prominent double-strand DNA cleavage. In addition, antioxidant activities of the ligand and its metal complexes investigated through scavenging effects for DPPH radical in- vitro, indicated their potentiality as good antioxidants. The in vitro anti-bacterial study inferred the better anti-bacterial activity of [Cu (II)-(L2)] and this was also correlated theoretically by employing docking studies wherein [Cu (II)-(L2)] displayed good Gold score and Chem score. Finally the in vitro anti- proliferative activity of studied compounds was tested against HeLa and MCF-7 cell lines. Interestingly [Cu (II)-(L2)] displayed lower IC50 value and lower percentage of viability in both HeLa and MCF-7 cell lines.
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India.
authors.forename: Kishan Prasad
authors.initials: KP
authors.lastname: Chennam
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India.
authors.forename: Mudavath
authors.initials: M
authors.lastname: Ravi
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India.
authors.forename: B
authors.initials: B
authors.lastname: Ushaiah
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India.
authors.forename: V
authors.initials: V
authors.lastname: Srinu
authors.affiliation: Department of Bio-Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India.
authors.forename: Ravi Kumar
authors.initials: RK
authors.lastname: Eslavath
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, Telangana, 500 007, India. dr_saraladevich@yahoo.com.
authors.forename: Ch Sarala
authors.initials: ChS
authors.lastname: Devi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001597:Benzylidene Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D053837:DNA Cleavage; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008670:Metals / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D062105:Molecular Docking Simulation; D015394:Molecular Structure; D009287:Naphthyridines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D001597:Benzylidene Compounds; D016166:Free Radical Scavengers; D008670:Metals; C000611345:N-(2-hydroxybenzylidene)-1-ethyl-1, 4-dihydro-7-methyl-4-oxo-1, 8 naphthyridine-3-carbohydrazide; D009287:Naphthyridines; D009942:Organometallic Compounds; D004247:DNA
keywords: Anti- proliferative activity; Anti-bacterial activity; DNA interactions; DNA nuclease activity; Docking studies; Radical scavenging