title: [The molecular mechanisms of platelets activation in patients with cerebrovascular disease].
abstract: Cerebrovascular disease is a main cause of mortality and one of the big medical problems. After the vascular wall's damage the endothelial cells secrete the von Willebrand factor which then connects with its platelet's receptor GP Ib-V-IX. There are two polymorphisms Thr145Met and T(-5)C of the GP Iba gene associated with arterial thrombosis development. Also the difference in platelets' genes expressions was shown in patients with various clinical course of ischemic heart disease. The aim of this study was to investigate the role of platelet's receptor for von Willebrand factor in platelets' activation in patients with cerebrovascular disease. 123 patients with cerebrovascular disease and 97 healthy donors were included into the study. We analyzed the level of receptor for von Willebrand factor on platelet's membrane by flow cytometry, Thr145Met and T(-5)C GP Iba polymorphiams by PCR-RFLP, the GP Iba gene expression by RT-PCR and ADP-induced platelet aggregation by Born method. We have shown: 1) the 145Met GP Iba allele prevalence in patients with atherotrombotic stroke development due to macroangiopathy; 2) the pre-mRNA transform into the mature mRNA in activated platelets and this process may be stopped by the antiplatelet therapy by acetylsalicylic acid.
authors.affiliation: National Research Centre "Kurchatov institute", B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russia; Federal Almazov North-West Medical Research Centre, St. Petersburg, Russia; Mechnikov North-Western State Medical University, St. Petersburg, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Sirotkina
authors.affiliation: Mechnikov North-Western State Medical University, St. Petersburg, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Laskovets
authors.affiliation: Mechnikov North-Western State Medical University, St. Petersburg, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Goldobin
authors.affiliation: Mechnikov North-Western State Medical University, St. Petersburg, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Topanova
authors.affiliation: National Research Centre "Kurchatov institute", B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, Russia; St.Petersburg State Polytechnical University, St. Petersburg, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Karelov
authors.affiliation: Federal Almazov North-West Medical Research Centre, St. Petersburg, Russia; Mechnikov North-Western State Medical University, St. Petersburg, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Vavilova
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000483:Alleles; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D003328:Coronary Thrombosis / Q000097:blood* / Q000150:complications / Q000235:genetics / Q000473:pathology; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D061251:Primary Cell Culture; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D020521:Stroke / Q000097:blood* / Q000150:complications / Q000235:genetics / Q000473:pathology; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism
chemical_list: D010975:Platelet Aggregation Inhibitors; D019038:Platelet Glycoprotein GPIb-IX Complex; D012322:RNA Precursors; C094990:adhesion receptor; D014841:von Willebrand Factor; D000244:Adenosine Diphosphate; D001241:Aspirin
keywords: GP Iba gene; and T(-5)C GP Iba gene polymorphisms; mature mRNA Iba; pre-mRNA Iba; receptor for von Willebrand factor