title: MicroRNA-766 targeting regulation of SOX6 expression promoted cell proliferation of human colorectal cancer.
abstract: MicroRNAs (miRNAs) have emerged as important regulators of cancer-cell biological processes. Previous studies have shown that miR-766 plays an important role in a variety of biological processes in various human cancers. However, the underlying mechanism of miR-766 in colorectal cancer (CRC) cells remains unclear. In this study, we investigated miR-766's role in CRC cell proliferation. Polymerase chain reaction results showed that miR-766 expression was significantly upregulated in CRC tissues and cells. Ectopic expression of miR-766 promoted cell growth and anchorage-independent growth in CRC cells. Bioinformatic analysis predicted SOX6, a potential target of miR-766, acting as a tumor suppressor. Luciferase reporter assay results demonstrated that miR-766 directly bound to the 3'-untranslated region of SOX6. Overexpression of miR-766 suppressed SOX6 expression, resulting in the downregulation of p21 and upregulation of cyclin D1. In a further experiment, SOX6-silenced SW480 cells transfected with miR-766 promoted cell growth, suggesting that downregulation of SOX6 was required for miR-766-induced CRC cell proliferation. Taken together, these results suggested that miR-766 represents an onco-miRNA and participates in the development of CRC by modulating SOX6 expression.
authors.affiliation: Department of Gastrointestinal Surgery, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Li
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Li
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Chen
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Li
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Jing-Peng
authors.initials: JP
authors.lastname: Jin
authors.affiliation: Department of Endoscopy Center, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
keywords: SOX6; cell proliferation; colorectal cancer; miR-766