title: Effect of nanofiber orientation of electrospun nanofibrous scaffolds on cell growth and elastin expression of muscle cells.
abstract: Tissue regeneration after smooth muscle tissue injury is a pivotal issue in tissue engineering. Good artificial scaffolds to continuously form long thin spindle-shaped smooth muscle cells in the damaged muscle tissues are important for tissue regeneration. In this work, poly(lactide-co-glycolide) (PLGA) and poly(ϵ-caprolactone) (PCL) were used to fabricate aligned or random electrospun nanofibrous scaffolds (ENSs) by using electrospinning technique. The cell growth and elastin expression of human vascular smooth muscle cells (HVSMCs) on these membranes were analyzed. Smooth PLGA/PCL film was used as control. The experimental results showed that the aligned ENS could maintain cell shapes of HVSMCs during the culture process. During the HVSMCs proliferation process, elastin expression firstly increase due to cell proliferation, and then decrease due to elastin degradation by elastase secreted by the cells. All these results suggest that aligned PLGA/PCL ENS can be a promising candidate for cell regeneration after smooth muscle tissue injury.
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China. Electronic address: jzhong@shou.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Iowa State University, Ames Laboratory, Ames, IA 50010, USA.
authors.forename: Huan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Chemical & Petroleum Engineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gong
mesh_terms: D002455:Cell Division; D004549:Elastin / Q000378:metabolism*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers
chemical_list: D004549:Elastin
keywords: Elastin; Electrospinning technique; Nanofiber orientation; Scaffolds; Smooth muscle cell