title: Biocompatibility of a novel zinc stent with a closed-cell-design.
abstract: Biomaterials made of zinc have been widely described to be antioxidative, hypothrombogenic, antiinflammatory and antiproliferative. Additionally in vivo zinc is toxic only in high concentrations and can completely be metabolized in vivo. Due to these properties zinc based vascular stents might be able to reduce the rate of restenosis in comparison to bare metal stents and zinc stents might be also able to limit the foreign body reaction. In the presented study we tested the biocompatibility and degradability of a stent made of zinc and characterized by a closed-cell-design to achieve high opening force and to increase stent stiffness. After 100 days of enzymatic and hydrolytic degradation in 15 ml blood serum (fetal calf serum) a significant loss of weight (1.72 wt% ) was measured. Zinc was compared to other metals in terms of degradation rates. After six weeks of incubation in physiologic sodium chloride solution zinc showed the slowest degradation time, 6 times less than stainless steel and 4 times less than magnesium. In the tests for cytotoxic effects the degraded zinc stent caused no changes in the LDH-release and cell membrane integrity (3T3 cells, mouse fibroblasts) respectively, in the cell activity/proliferation (MTS assay) and in the morphological characteristics of the cells and cell layers in comparison to the control material (polystyrene). Based on these results the tested zinc stent proved to be non-cytotoxic and to be characterized by degradation characteristics which might be advantageous in comparison to magnesium and stainless steel.
authors.affiliation: Center for Medical Basic Research, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Hiebl
authors.affiliation: OPTIMED Medizinische Instrumente GmbH, Ettlingen, Germany.
authors.forename: Ernst
authors.initials: E
authors.lastname: Nennig
authors.affiliation: Limedion GmbH, Mannheim, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schiestel
authors.affiliation: Limedion GmbH, Mannheim, Germany.
authors.forename: Adalbert
authors.initials: A
authors.lastname: Kovacs
authors.affiliation: Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Kantstrasse, Teltow, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Jung
authors.affiliation: CREAMEDIX GmbH, Weingarten, Germany.
authors.forename: Harald
authors.initials: H
authors.lastname: Fischer
mesh_terms: D001672:Biocompatible Materials; D011474:Prosthesis Design; D015607:Stents; D015032:Zinc
chemical_list: D001672:Biocompatible Materials; D015032:Zinc
keywords: biocompatibility; degradation rate; stent; zinc