title: Effects of fluoride-ion-implanted titanium surface on the cytocompatibility in vitro and osseointegatation in vivo for dental implant applications.
abstract: As an attractive technique for the improvement of biomaterials, Plasma immersion ion implantation (PIII) has been applied to modifying the titanium material for dental implant application. The present study investigated the cytocompatibility and early osseointegration of fluoride-ion-implanted titanium (F-Ti) surface and implants, both characterizing in their composition of titanium oxide and titanium fluoride. The cytocompatibility of F-Ti was evaluated in vitro by using scanning electron microscope, Cell Counting Kit-8 assay, alkaline phosphatase activity assay, and quantitative real-time polymerase chain reaction. The results showed that the F-Ti weakened the effects that Porphyromonas gingivalis exerted on the MG-63 cells in terms of morphology, proliferation, differentiation, and genetic expression when MG-63 cells and Porphyromonas gingivalis were co-cultured on the surface of F-Ti. Meanwhile, the osteogenic activity of F-Ti implants was assessed in vivo via evaluating the histological morphology and estimating histomorphometric parameters. The analysis of toluidine blue staining indicated that the new bone was more mature in subjects with F-Ti group, which exhibited the Haversian system, and the mean bone-implant contact value of F-Ti group was slightly higher than that of cp-Ti group (p>0.05). Fluorescence bands were wider and brighter in the F-Ti group, and the intensity of fluorochromes deposited at the sites of mineralized bone formation was significantly higher for F-Ti surfaces than for cp-Ti surfaces, within the 2nd, 3rd and 4th weeks (p<0.05). An indication is that the fluoride modified titanium can promote cytocompatibility and early osseointegration, thus providing a promising alternative for clinical use.
authors.affiliation: Department of Stomatology, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, PR China.
authors.forename: Xue-jin
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China. Electronic address: lhy04512000@163.com.
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Liu
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ren
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Hui-yan
authors.initials: HY
authors.lastname: Sun
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Zhu
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Ying
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Shu-hai
authors.initials: SH
authors.lastname: Hu
authors.affiliation: Laboratory Animal Center, Dalian Medical University, Dalian 116044, PR China.
authors.forename: Ze-wen
authors.initials: ZW
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Advanced Welding Production Technology, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Lang-ping
authors.initials: LP
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Advanced Welding Production Technology, Harbin Institute of Technology, Harbin 150001, PR China.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Wang
authors.affiliation: School of Stomatology, Dalian Medical University, Dalian 116044, PR China. Electronic address: mgw64024@163.com.
authors.forename: Guo-wu
authors.initials: GW
authors.lastname: Ma
mesh_terms: D001672:Biocompatible Materials; D002460:Cell Line; D015921:Dental Implants; D005459:Fluorides / Q000737:chemistry*; D006801:Humans; D016348:Osseointegration; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium; D005459:Fluorides
keywords: Cytocompatibility; Dental implant; Fluoride; Osseointegration; Plasma immersion ion implantation; Titanium