title: Application of flow cytometry for myelodysplastic syndromes: Pitfalls and technical considerations.
abstract: The application of flow cytometry (FC) is recommended as part of the diagnostic approach for MDS. The complexity of flow cytometric analysis of bone marrow cells in MDS has been an obstacle for general application. However, in the past years several studies showed practical flow cytometric approaches for the diagnosis and prognosis of MDS. In this report we discuss technical considerations and highlight issues that require special attention when handling and analyzing bone marrow samples of patients with cytopenia and suspicion of MDS. © 2015 Clinical Cytometry Society.
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Canan
authors.initials: C
authors.lastname: Alhan
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Theresia M
authors.initials: TM
authors.lastname: Westers
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Eline M P
authors.initials: EM
authors.lastname: Cremers
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cali
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Gert J
authors.initials: GJ
authors.lastname: Ossenkoppele
authors.affiliation: Department of Hematology, Cancer Center Amsterdam (CCA), VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Arjan A
authors.initials: AA
authors.lastname: van de Loosdrecht
mesh_terms: D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D009190:Myelodysplastic Syndromes / Q000175:diagnosis* / Q000473:pathology*; D011379:Prognosis
keywords: flow cytometry; myelodysplastic syndromes; pitfalls