title: Development of a biointegrated mandibular reconstruction device consisting of bone compatible titanium fiber mesh scaffold.
abstract: Coating biomaterials with a thin hydroxyapatite (HA) was proven effective in enhancing bone compatibility. Segmental bone defects are considered as the most difficult defect to repair in bone regeneration therapy. We developed submicron-thin HA-coated titanium fiber mesh scaffolds to reconstruct immediately loaded segmental mandibular defects and evaluated their bone compatibility in vitro and in vivo. Human osteoblasts attachment, proliferation, and osteocalcin expression in non- and HA-coated scaffolds were evaluated. A 10-mm long segmental bone defect in a rabbit mandibular bone was reconstructed with non- or HA-coated scaffolds, which were removed at 9 and 21 weeks, to evaluate the mechanical strength of the bone-scaffold connection and the bone formation around the scaffold. Expression of osteocalcin was greater in HA-coated scaffolds. In vivo bone formation in HA-coated scaffolds was greater than that in non-coated scaffolds at 21 weeks. Newly formed bone in HA-coated scaffolds mostly restored bone continuity. Scanning electron microscopy identified strong integration of the bone and HA-coated scaffolds. The mechanical strength of the bone-scaffold connection was 3-fold greater in HA-coated scaffolds than that in non-coated scaffolds. These results suggest that a thin HA-coated titanium fiber mesh scaffold is a bone-compatible mandibular reconstruction device in immediately loaded segmental defects.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan. Electronic address: mhirota@med.yokohama-cu.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirota
authors.affiliation: Medical Device Department, HI-LEX Corporation, Inc, 1-12-28 Sakae-cho, Takaraduka, 665-0845, Japan.
authors.forename: Takaki
authors.initials: T
authors.lastname: Shima
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Itaru
authors.initials: I
authors.lastname: Sato
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Tomomichi
authors.initials: T
authors.lastname: Ozawa
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Iwai
authors.affiliation: Medical Device Department, HI-LEX Corporation, Inc, 1-12-28 Sakae-cho, Takaraduka, 665-0845, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ametani
authors.affiliation: Department of Applied Physics, School of Advanced Engineering, Kogakuin University, 1-24-2 Nishi-Shinjjuku, Tokyo, 163-8677, Japan.
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Sato
authors.affiliation: Division of Artificial Organ Science, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Noishiki
authors.affiliation: Laboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, 10833 Le Conte Avenue, Box 951668, Los Angeles, CA, 90095-1668, USA.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Department of Dental Engineering, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, 230-8501, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Hayakawa
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: Iwai
authors.initials: I
authors.lastname: Tohnai
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology; D006801:Humans; D008297:Male; D008334:Mandible / Q000033:anatomy & histology / Q000000981:diagnostic imaging / Q000601:surgery* / Q000648:ultrastructure; D063175:Mandibular Reconstruction / Q000295:instrumentation*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: D020099:Coated Materials, Biocompatible; C101914:titanium fiber; D017886:Durapatite; D014025:Titanium
keywords: Hydroxyapatite coating; Mandibular reconstruction; Scaffold; Titanium fiber mesh