title: Transcriptome changes in STSV2-infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition.
abstract: A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, whereas those of DNA replication, DNA repair, transcriptional regulation and some antitoxin-toxin pairs and transposases were unchanged or enhanced. Antisense RNAs were implicated in the transcriptional regulation of adaptation and interference modules of the type I-A CRISPR-Cas system, and evidence was found for the occurrence of functional co-ordination between the single CRISPR-Cas adaptation module and the functionally diverse interference modules.
authors.affiliation: Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, DK-2200, Denmark.
authors.forename: Carlos
authors.initials: C
authors.lastname: León-Sobrino
authors.affiliation: Department of Environmental Science, Aarhus University, Roskilde, Denmark.
authors.forename: Witold P
authors.initials: WP
authors.lastname: Kot
authors.affiliation: Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen, DK-2200, Denmark.
authors.forename: Roger A
authors.initials: RA
authors.lastname: Garrett
mesh_terms: D023641:Archaeal Viruses / Q000235:genetics*; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D019848:Gene Expression Regulation, Archaeal; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D016944:Sulfolobus / Q000235:genetics* / Q000254:growth & development / Q000821:virology*; D015533:Transcriptional Activation; D059467:Transcriptome; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral