title: Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia coli Cells with Single-Molecule Sensitivity.
abstract: Single-cell proteomic and transcriptomic analysis is an emerging approach for providing quantitative and comprehensive characterization of gene functions in individual cells. This analysis, however, is often hampered by insufficient sensitivity for detecting low copy gene expression products such as transcription factors and regulators. Here I describe a method for the quantitative genome-wide analysis of single-cell protein and mRNA copy numbers with single molecule sensitivity for the model organism Escherichia coli.
authors.affiliation: Laboratory for Single Cell Gene Dynamics, Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka, 5650874, Japan. taniguchi@riken.jp.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Taniguchi
mesh_terms: D004867:Equipment Design; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D018628:Gene Dosage; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D013269:Stochastic Processes
chemical_list: D029968:Escherichia coli Proteins; D012333:RNA, Messenger
keywords: Escherichia coli; Fluorescent protein fusion library; High-throughput platform; Microfluidics; Single molecule detection; Single-cell proteomic and transcriptomic analysis; Stochastic gene expression