title: Ultrastructure and molecular phylogeny of Pleistophora hyphessobryconis (Microsporidia) infecting hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a Brazilian aquaculture facility.
abstract: A microsporidian infecting the skeletal muscle of hybrid jundiara (Leiarius marmoratus × Pseudoplatystoma reticulatum) in a commercial aquaculture facility in Brazil is described. Affected fish exhibited massive infections in the skeletal muscle that were characterized by large opaque foci throughout the affected fillets. Histologically, skeletal muscle was replaced by inflammatory cells and masses of microsporidial developmental stages. Generally pyriform spores had a wrinkled bi-layer spore wall and measured 4·0 × 6·0 µm. Multinucleate meronts surrounded by a simple plasma membrane were observed. The polar filament had an external membrane and a central electron dense mass. The development of sporoblasts within a sporophorous vesicle appeared synchronized. Ultrastructural observations and molecular analysis of 16S rDNA sequences revealed that the microsporidian was Pleistophora hyphessobryconis. This study is the first report of a P. hyphessobryconis infection in a non-ornamental fish.
authors.affiliation: Department of Fisheries and Wildlife,Michigan State University,East Lansing,Michigan 48824,USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Winters
authors.affiliation: Department of Pathobiological Sciences,Louisiana State University,Baton Rouge,Louisiana 70803,USA.
authors.forename: Ingeborg M
authors.initials: IM
authors.lastname: Langohr
authors.affiliation: Clinical Veterinary Medical Department,Faculty of Agronomy,Veterinary Medicine and Animal Science,Federal University of Mato Grosso,Cuiabá,Mato Grosso,Brazil.
authors.forename: Marcos D E A
authors.initials: MD
authors.lastname: Souza
authors.affiliation: Clinical Veterinary Medical Department,Faculty of Agronomy,Veterinary Medicine and Animal Science,Federal University of Mato Grosso,Cuiabá,Mato Grosso,Brazil.
authors.forename: Edson M
authors.initials: EM
authors.lastname: Colodel
authors.affiliation: Regional Diagnostic Laboratory,Federal University of Pelotas,Pelotas,RS,Brazil.
authors.forename: Mauro P
authors.initials: MP
authors.lastname: Soares
authors.affiliation: Department of Fisheries and Wildlife,Michigan State University,East Lansing,Michigan 48824,USA.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Faisal
mesh_terms: D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D001938:Brazil; D002397:Catfishes / Q000382:microbiology*; D002678:Chimera; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology* / Q000473:pathology; D016881:Microsporidiosis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D018482:Muscle, Skeletal / Q000382:microbiology; D010802:Phylogeny; D021864:Pleistophora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal
keywords: 16S rDNA; Microsporidia; Pleistophora hyphessobryconis; aquaculture