title: Morphologic and Genotypic Characterization of Psoroptes Mites from Water Buffaloes in Egypt.
abstract: Species delimitation of Psoroptes spp. and identity of the parasite in water buffaloes remain poorly defined. In this study, Psoroptes infestation on three water buffalo farms in Egypt was examined based on morphometric characteristics, especially the opisthosomal setae of adult male mites. Clinical investigations showed that 28% (196/700) of the sampled animals had mange infestation. Microscopic examinations of 80 skin scrapings indicated the occurrence of Psoroptes mites in 17 (21.3%) samples, Sarcoptes mites in 27 (33.7%) samples, and the concurrence of both in 36 (45.0%) samples. Morphologically, the Psoroptes parasite was identified as Psoroptes natalensis. DNA sequence analysis of the second internal transcribed spacer (ITS2) in 11 representative samples confirmed the diagnosis and suggested the presence of a distinct variety of Psoroptes natalensis in Egypt.
authors.affiliation: Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafr El Sheikh, Egypt.
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.affiliation: Animal Health Research Institute, Kafr El Sheikh Provisional Laboratory, Kafr El Sheikh, Egypt.
authors.forename: Taher
authors.initials: T
authors.lastname: Abd El Wahab
authors.affiliation: Animal Health Research Institute, Kafr El Sheikh Provisional Laboratory, Kafr El Sheikh, Egypt.
authors.forename: Abd
authors.initials: A
authors.lastname: El Naby Metwaly
authors.affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D015897:Comorbidity; D004247:DNA / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D008924:Mite Infestations / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D040042:Psoroptidae / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D012532:Scabies / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D004247:DNA