title: SRYTH: A New Yeast Two-Hybrid Method.
abstract: Many biological processes are regulated by protein-protein interactions, and the analysis of these interactions has been a productive endeavor contributing to our understanding of cellular organization and function. The yeast two-hybrid technique is a widely used, powerful method of analyzing protein-protein interactions. The currently used formats, however, have inherent limitations, providing an opportunity to develop new alternatives that extend our ability to detect protein-protein interactions of biological relevance. Here we present a two-hybrid system named SRYTH (Ste11p/Ste50p related yeast two-hybrid) based on the Ste11p/Ste50p interaction that uses the activation of the HOG pathway of Saccharomyces cerevisiae as a reporter for interactions. The system is suitable for detecting cytoplasmic protein interactions in their natural subcellular environment, and has been successfully used to investigate protein-protein interactions, including transcription factor associations, in Candida albicans.
authors.affiliation: Biology Department, Concordia University, 7141 Sherbrooke St. West, Montreal, QC, Canada, H4B 1R6.
authors.forename: Jaideep
authors.initials: J
authors.lastname: Mallick
authors.affiliation: Biochemistry Department, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, Canada, H3G 1Y6.
authors.forename: Gregor
authors.initials: G
authors.lastname: Jansen
authors.affiliation: National Research Council of Canada, 6100 Royalmount Ave, Montreal, QC, Canada, H4P 2R2.|Division of Experimental Medicine, McGill University, 1100 Pine Ave.West, Montreal, QC, Canada, H3A 1A3.
authors.forename: Cunle
authors.initials: C
authors.lastname: Wu
authors.affiliation: Biology Department, Concordia University, 7141 Sherbrooke St. West, Montreal, QC, Canada, H4B 1R6. Malcolm.whiteway@concordia.ca.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Whiteway
mesh_terms: D025941:Protein Interaction Mapping / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D020798:Two-Hybrid System Techniques
chemical_list: D029701:Saccharomyces cerevisiae Proteins
keywords: Candida albicans; HOG pathway; Protein-protein interaction; Yeast two-hybrid