title: A novel signal amplification strategy of an electrochemical aptasensor for kanamycin, based on thionine functionalized graphene and hierarchical nanoporous PtCu.
abstract: An ultrasensitive electrochemical aptasensor for the quantitative detection of kanamycin antibiotic was fabricated based on a novel signal amplification strategy. This aptasensor was developed using thionine functionalized graphene (GR-TH) and hierarchical nanoporous (HNP) PtCu alloy as biosensing substrates for the first time. HNP-PtCu alloy with controllable bimodal ligament/pore distributions was successfully prepared by two-step dealloying of a well-designed PtCuAl precursor alloy combined with an annealing operation. GR-TH composite was synthesized by one-step reduction of graphene oxide (GO) in TH solution. Greatly amplified sensitivity was achieved by using GR-TH/HNP-PtCu composite owing to its large specific surface and good electron-transfer ability. Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to kanamycin in the range 5 × 10(-7)-5 × 10(-2) μgmL(-1) with a low detection limit of 0.42 pgmL(-1). This aptasensor also displayed a satisfying electrochemical performance with good stability, selectivity and reproducibility. The as-prepared aptasensor was successfully used for the determination of kanamycin in animal derived food.
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qin
authors.affiliation: School of Environmental science and Engineering, Qilu University of Technology, Jinan 250353, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Wendeng Osteopath Hosptical of Shandong Province, Weihai 264400, China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Yu
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address: chm_guowj@163.com.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Shandong Provincial Key Laboratory of Chemical Sensing & Analysis, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Meishan
authors.initials: M
authors.lastname: Pei
mesh_terms: D000497:Alloys / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D003300:Copper / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis; D006108:Graphite / Q000737:chemistry; D007612:Kanamycin / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure*; D058608:Nanopores / Q000648:ultrastructure; D010640:Phenothiazines / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D010640:Phenothiazines; D010984:Platinum; D007612:Kanamycin; D006108:Graphite; D003300:Copper; C009469:thionine
keywords: Electrochemical aptasensor; Kanamycin; Platinum; Thionine