title: Isolation of flavonoids from Delonix elata and determination of its rutin content using capillary electrophoresis.
abstract: Delonix elata (L.) Gamble (Fabaceae) is an important, traditionally used plant in Saudi Arabia. It is used to relieve rheumatic pain, flatulence and the seeds are employed as purgatives. The aim of the present study was to isolate chemical constituents of the n-butanol fraction (BF) of D. elata and to find out, by capillary electrophoresis (CE), percentage of rutin present in this BF. Three quercetin glycosides and one kaempferol rutinoside were isolated from the BF of aerial parts of D. elata; namely, Quercetin 3-O-rutinoside-7-O-glucoside (1), Quercetin 3,7-diglucoside (2), Quercetin 3-O-rutinoside (RUT) (3) and Kaempferol 3-O-rutinoside (4). Rutin, an active constituent has been reported to possess good pharmacological as well as therapeutic potentials. A sensitive and rapid procedure for quantitative determination of RUT by capillary electrophoresis was developed and its content was found to be 7.349 mg/gm, relative to n-butanol fraction and 18.373 mg%, relative to the dry powder of D. elata. The method could be recommended for approval and use in the pharmaceutical and food industries.
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Areej Mohammed
authors.initials: AM
authors.lastname: Al-Taweel
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, Saudi Arabia / Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
authors.forename: Maged Saad
authors.initials: MS
authors.lastname: Abdel-Kader
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia / Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Ghada Ahmed
authors.initials: GA
authors.lastname: Fawzy
authors.affiliation: Pharmacognosy Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Shagufta
authors.initials: S
authors.lastname: Perveen
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia / Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Alexandria University, El-Messalah, Alexandria, Egypt.
authors.forename: Hadir Mohamed
authors.initials: HM
authors.lastname: Maher
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Nourah Zoman
authors.initials: NZ
authors.lastname: Al-Zoman
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mona Mohamed
authors.initials: MM
authors.lastname: Al-Shehri
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Haya
authors.initials: H
authors.lastname: Al-Johar
authors.affiliation: Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Hessa
authors.initials: H
authors.lastname: Al-Showiman
mesh_terms: D000440:Butanols; D002138:Calibration; D019075:Electrophoresis, Capillary; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification*; D006868:Hydrolysis; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis*; D012997:Solvents
chemical_list: D000440:Butanols; D005419:Flavonoids; D010936:Plant Extracts; D012997:Solvents; D012431:Rutin