title: Preparation of Cell Cultures and Vaccinia Virus Stocks.
abstract: The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included.
authors.affiliation: National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Cotter
authors.affiliation: National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Earl
authors.affiliation: National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Linda S
authors.initials: LS
authors.lastname: Wyatt
authors.affiliation: National Institutes of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004279:DNA, Viral / Q000302:isolation & purification; D011309:Preservation, Biological / Q000379:methods*; D014616:Vaccinia virus / Q000254:growth & development* / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: cell culture; modified vaccinia virus Ankara; vaccinia virus