title: Murine Monoclonal Antibodies for Antigenic Discrimination of HIV-1 Envelope Proteins.
abstract: In the influenza virus field, antibody reagents from research animals have been instrumental in the characterization of antigenically distinct hemagglutinin and neuraminidase membrane molecules. These small animal reagents continue to support the selection of components for inclusion in human influenza virus vaccines. Other cocktail vaccines against variant pathogens (e.g., polio virus, pneumococcus) are similarly designed to represent variant antigens, as defined by antibody reactivity patterns. However, a vaccine cocktail comprising diverse viral membrane antigens defined in this way has not yet been advanced to a clinical efficacy study in the HIV-1 field. In this study, we describe the preparation of mouse antibodies specific for HIV-1 gp140 or gp120 envelope molecules. Our experiments generated renewable reagents able to discriminate HIV-1 envelopes from one another. Monoclonals yielded more precise discriminatory capacity against their respective immunogens than did a small panel of polyclonal human sera derived from recently HIV-1-infected patients. Perhaps these and other antibody reagents will ultimately support high-throughput cartography studies with which antigenically-distinct envelope immunogens may be formulated into a successful HIV-1 envelope cocktail vaccine.
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Sealy
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.
authors.forename: Bart G
authors.initials: BG
authors.lastname: Jones
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.
authors.forename: Sherri L
authors.initials: SL
authors.lastname: Surman
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.
authors.forename: Kristen
authors.initials: K
authors.lastname: Branum
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.
authors.forename: Nanna M
authors.initials: NM
authors.lastname: Howlett
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.|2 Department of Pediatrics, University of Tennessee Health Science Center , Memphis, Tennessee.|3 Department of Preventive Medicine, University of Tennessee Health Science Center , Memphis, Tennessee.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Flynn
authors.affiliation: 1 Department of Infectious Diseases, St. Jude Children's Research Hospital , Memphis, Tennessee.|4 Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center , Memphis, Tennessee.
authors.forename: Julia L
authors.initials: JL
authors.lastname: Hurwitz
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D054299:env Gene Products, Human Immunodeficiency Virus; C514793:gp120 protein, Human immunodeficiency virus 1; C097729:gp140 envelope protein, Human immunodeficiency virus 1