title: Surface modification of polytetrafluoroethylene column for two-stationary phase separations by counter-current chromatography.
abstract: To improve the separation capability of CCC, a novel solid-liquid two-stationary phases CCC (ASP-CCC) column was prepared employing graphene oxide (GO) conjugated poly-dopamine (PD) coating (GO/PD) as auxiliary stationary phase (ASP). The results of Scanning electron microscopy (SEM), contact angle and X-ray photoelectron spectroscopy (XPS) indicated that nanostructured GO and PD were successfully grafted on the inner wall of the PTFE column. Three alkaloid compounds were selected as the target analytes to evaluate the performance of the novel column. Because of the intermolecular force (hydrogen bond, electrostatic interaction and π-π interaction) between the ASP and model compounds, three analytes were well separated with this novel ASP-CCC column. Additionally, the novel column exhibited higher stationary phase retention ratio, about 8%, than original column without changing the chromatographic condition. Furthermore, the eluotropic sequence of analytes on novel column was in accordance with that in the original column. This suggested that the novel column is a CCC column with auxiliary stationary phase (ASP) in its own right, and the present separation mode is the combination of partition chromatography and adsorption chromatography.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Kai-Jun
authors.initials: KJ
authors.lastname: Quan
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China.
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Huang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Xiao-Ting
authors.initials: XT
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Gao-Hong
authors.initials: GH
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Yan-Juan
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
authors.forename: Wen-da
authors.initials: WD
authors.lastname: Duan
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, No. 18. Tianshui Middle Road, Lanzhou, 730000, China. Electronic address: didl@licp.cas.cn.
authors.forename: Duo-Long
authors.initials: DL
authors.lastname: Di
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002853:Chromatography, Liquid; D006108:Graphite / Q000737:chemistry; D010087:Oxides; D056951:Photoelectron Spectroscopy; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D010087:Oxides; D006108:Graphite; D011138:Polytetrafluoroethylene
keywords: Adsorption; Alkaloid; Auxiliary stationary phase; CCC; Graphene oxide; Poly-dopamine