title: The syntaxin protein (MoSyn8) mediates intracellular trafficking to regulate conidiogenesis and pathogenicity of rice blast fungus.
abstract: Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) mediate cellular membrane fusion and intracellular vesicle trafficking in eukaryotic cells, and are critical in the growth and development of pathogenic fungi such as Magnaporthe oryzae which causes rice blast. Rice blast is thought to involve distinct SNARE-mediated transport and secretion of fungal effector proteins into the host to modulate rice immunity. We have previously characterized two SNARE proteins, secretory protein (MoSec22) and vesicle-associated membrane protein (MoVam7), as being important in cellular transport and pathogenicity. Here, we show that syntaxin 8 (MoSyn8), a Qc-SNARE protein homolog, also plays important roles in growth, conidiation, and pathogenicity. The MoSYN8 deletion mutant (∆Mosyn8) mutant exhibits defects in endocytosis and F-actin organization, appressorium turgor pressure generation, and host penetration. In addition, the ∆Mosyn8 mutant cannot elaborate biotrophic invasion of the susceptible rice host, or secrete avirulence factors Avr-Pia (corresponding to the rice resistance gene Pia) and Avrpiz-t (the cognate Avr gene for the resistance gene Piz-t) proteins. Our study of MoSyn8 advances our understanding of SNARE proteins in effector secretion which underlies the normal physiology and pathogenicity of M. oryzae, and it sheds new light on the mechanism of the blight disease caused by M. oryzae.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Zhongqiang
authors.initials: Z
authors.lastname: Qi
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Muxing
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Yanhan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Lianwei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ru
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, LA, 70118, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, Jiangsu, 210095, China.
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D001692:Biological Transport; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D042541:Intracellular Space / Q000378:metabolism*; D020082:Magnaporthe / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity*; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D011312:Pressure; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D012098:Reproduction; D013172:Spores, Fungal / Q000378:metabolism*; D014774:Virulence / Q000235:genetics
chemical_list: D000199:Actins; D005656:Fungal Proteins; D050765:Qa-SNARE Proteins
keywords: Magnapother oryzae; Qc-SNARE; conidiogenesis; endocytosis; pathogenesis; secretion