title: Beneficial effects of low dose radiation in response to the oncogenic KRAS induced cellular transformation.
abstract: Recently low dose irradiation has gained attention in the field of radiotherapy. For lack of understanding of the molecular consequences of low dose irradiation, there is much doubt concerning its risks on human beings. In this article, we report that low dose irradiation is capable of blocking the oncogenic KRAS-induced malignant transformation. To address this hypothesis, we showed that low dose irradiation, at doses of 0.1 Gray (Gy); predominantly provide defensive response against oncogenic KRAS -induced malignant transformation in human cells through the induction of antioxidants without causing cell death and acts as a critical regulator for the attenuation of reactive oxygen species (ROS). Importantly, we elucidated that knockdown of antioxidants significantly enhanced ROS generation, invasive and migratory properties and abnormal acini formation in KRAS transformed normal as well as cancer cells. Taken together, this study demonstrates that low dose irradiation reduces the KRAS induced malignant cellular transformation through diminution of ROS. This interesting phenomenon illuminates the beneficial effects of low dose irradiation, suggesting one of contributory mechanisms for reducing the oncogene induced carcinogenesis that intensify the potential use of low dose irradiation as a standard regimen.
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Rae-Kwon
authors.initials: RK
authors.lastname: Kim
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Ki Moon
authors.initials: KM
authors.lastname: Seong
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Neha
authors.initials: N
authors.lastname: Kaushik
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Yongjoon
authors.initials: Y
authors.lastname: Suh
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Ki-Chun
authors.initials: KC
authors.lastname: Yoo
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Cui
authors.affiliation: Laboratory of Radiation Exposure and Therapeutics, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
authors.forename: Young Woo
authors.initials: YW
authors.lastname: Jin
authors.affiliation: Radiation Health Institute, Korea Hydro and Nuclear Power Co. Ltd, Seoul, Korea.
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Nam
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002374:Catalase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D058750:Epithelial-Mesenchymal Transition; D005720:Gamma Rays; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D018631:ras Proteins