title: The hetero-transplantation of human bone marrow stromal cells carried by hydrogel unexpectedly demonstrates a significant role in the functional recovery in the injured spinal cord of rats.
abstract: Spinal cord injury (SCI) often causes a disturbance in the microenvironment in the lesion site resulting in sudden loss of sensory and motor function. Transplantation of stem cells provides a promising strategy in the treatment of SCI. But limited growth and immunological incompatibility of the stem cells with the host limits the application of this strategy. In order to get better survival and integration with the host, we employed a hyaluronic acid (HA) based scaffold covalently modified by poly-l-Lysine (PLL) as a vehicle to deliver the human bone marrow stromal cells (BMSCs) to the injured spinal cord of rats. The BMSCs were chosen as an ideal candidate for its advantage of low expression of major histocompatibility complex II. The data unexpectedly showed that the hetero-transplanted cells survived well in the lesion site even at 8 weeks post injury. Both the immunofluorescent and the electrophysiological assay indicated better survival of the transplanted cells and improved axonal growth in SCI rats transplanted with BMSCs in HA-PLL in contrast to the groups without either BMSCs or the HA scaffold transplantation. These promotions may account for the functional recovery assessed by Basso-Beattie-Bresnahan (BBB) locomotor rating scale in the HA-PLL seeded with BMSCs group. These data suggests that hetero-transplantation of human BMSCs delivered by HA scaffold demonstrates a significant role in the functional recovery in the injured spinal cord of rats.
authors.affiliation: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China. Electronic address: raynald_liu@yahoo.com.
authors.lastname: Raynald
authors.affiliation: Beijing Di Tan Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: Biomaterials Laboratory, Department of Materials Science and Engineering, TsingHua University, Beijing 100054, China.
authors.forename: Muyao
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Stem Cell Transplantation, The General Hospital of Chinese People׳s Armed Police Forces, Beijing 100039, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Hua
authors.affiliation: Department of Stem Cell Transplantation, The General Hospital of Chinese People׳s Armed Police Forces, Beijing 100039, China.
authors.forename: Fenjun
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors.forename: Kaihua
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Beijing Neurosurgical Institute, Capital Medical University, Beijing 100050, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Inner Mongolia Medical University Affiliated Hospital, Inner Mongolia 010050, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Li
authors.affiliation: Biomaterials Laboratory, Department of Materials Science and Engineering, TsingHua University, Beijing 100054, China.
authors.forename: FuZhai
authors.initials: F
authors.lastname: Cui
authors.affiliation: Department of Stem Cell Transplantation, The General Hospital of Chinese People׳s Armed Police Forces, Beijing 100039, China; Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, China. Electronic address: doctoran2010@hotmail.com.
authors.forename: Yihua
authors.initials: Y
authors.lastname: An
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid; D020100:Hydrogels; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D009043:Motor Activity; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D011107:Polylysine / Q000031:analogs & derivatives; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000601:surgery*; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D011107:Polylysine; D006820:Hyaluronic Acid
keywords: Bone marrow stromal cell; Functional recovery; Hetero-transplantation; Hyaluronic acid; Spinal cord injury