title: Bioluminescence assay for cell viability.
abstract: Theoretical aspects of the adenosine triphosphate bioluminescence assay based on the use of the firefly luciferin-luciferase system are considered, as well as its application for assessing cell viability in microbiology, sanitation, medicine, and ecology. Various approaches for the analysis of individual or mixed cultures of microorganisms are presented, and capabilities of the method for investigation of biological processes in live cells including necrosis, apoptosis, as well as for investigation of the dynamics of metabolism are described.
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia. nugarova@gmail.com.
authors.forename: G Yu
authors.initials: GY
authors.lastname: Lomakina
authors.forename: Yu A
authors.initials: YA
authors.lastname: Modestova
authors.forename: N N
authors.initials: NN
authors.lastname: Ugarova
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D049454:Firefly Luciferin / Q000737:chemistry; D049409:Luciferases, Firefly / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism
chemical_list: D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D049409:Luciferases, Firefly