title: [Identification of A Novel HLA Allele DRB1 * 16:36 by Sequencing-Based Typing].
abstract: OBJECTIVE
  To identify a novel HLA allele DRB1 * 16:36 from a Uygur woman.
  
  
  METHODS
  PCR-SBT technology was applied to the extracted DNA for genotyping, and a possible new gene was sequenced by using sequence specific primers and single stranded SBT. This novel allele was compared with known most homologous gene sequences and their difference was analyzed.
  
  
  RESULTS
  This novel allele was different from HLA alle DRB1 * 16:23, and had highest similarity in 2 nucleotides at position 227 A→T and 236 T→C in exon 2, resulting in 3 amino acid changes from Tyr to Phe at codon 47 and Val to Ala at codon 50. The sequence of this novel allele had been submitted to GenBank.
  
  
  CONCLUSIONS
  This HLA allele DRB1 * 16:23 has been confirmed to be a novel allele, and has been officially named DRB1 * 16:36 by the World Health Organization (WHO) Nomenclature Committee in May 2015.
authors.affiliation: State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China.|Department of Infectiou Disease, The First Affiliated Hospital of Shihezi University Medical College, Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Lu
authors.affiliation: Department of Infectiou Disease, The First Affiliated Hospital of Shihezi University Medical College, Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
authors.forename: Wei-Ze
authors.initials: WZ
authors.lastname: Zuo
authors.affiliation: State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
authors.forename: Wan-Jiang
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory, Ministry of Education, Shihezi University Medical College; Shihezi 832000, Xinjiang Uygur Autonomous Region, China. E-mail: wujiangdong@shzu.edu.cn.
authors.forename: Jiang-Dong
authors.initials: JD
authors.lastname: Wu
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D005260:Female; D005838:Genotype; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D059811:HLA-DRB1 Chains