title: Propofol promotes cell apoptosis via inhibiting HOTAIR mediated mTOR pathway in cervical cancer.
abstract: OBJECTIVE
  Cervical cancer is one of the most common gynecologic malignant tumors. Propofol has been proposed to play a role of antitumor in various cancers. However, the functions and mechanisms of Propofol in cervical cancer is still not clear.
  
  
  METHODS
  In vitro, the different concentrations of propofol were co-incubated with cervical cancer cell lines, including Hela, Caski and C-33A cells respectively. The pcDNA-HOTAIR plasmid was transfected into cells after the treatment of 10 μg/ml propofol. The cell viability and apoptosis were detected by MTT assay and TUNEL method. In vivo, propofol was injected into mice of transplantation tumor with Caski cells or with pcDNA-HOTAIR treated Caski cells.
  
  
  RESULTS
  Propofol significantly decreased the cell viability and increased the cell apoptosis in Hela, Caski and C-33A cells, while HOTAIR overexpression promoted cell viability and inhibits cell apoptosis. mTOR/p70S6K protein expression levels were also markedly reduced by propofol but the effects were reversed with pcDNA-HOTAIR. In vivo, propofol inhibited the tumor size but had no inhibition effect in HOTAIR overexpression group.
  
  
  CONCLUSIONS
  Propofol inhibited tumor size, cell viability and promoted cell apoptosis via inhibiting mTOR/p70S6K pathway mediated by HOTAIR in cervical cancer.
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Xin-hui
authors.initials: XH
authors.lastname: Zhou
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Yun-xiao
authors.initials: YX
authors.lastname: Zhou
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ying
authors.affiliation: Department of Oncology, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Wu
authors.affiliation: Department of Gynecology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. Electronic address: jianhuaq0@163.com.
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Qian
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D015742:Propofol / Q000008:administration & dosage*; D062085:RNA, Long Noncoding / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding; C546843:mTOR protein, mouse; D058570:TOR Serine-Threonine Kinases; D015742:Propofol
keywords: Cervical cancer; Propofol; lncRNAs; mTOR