title: Prognostic value of MAGE-A9 expression in patients with colorectal cancer.
abstract: MAGE-A9 is a novel member of the melanoma-associated antigen (MAGE) family and is expressed in testicular cancer. The present study investigated MAGE-A9 expression as a potential biomarker in colorectal cancer (CRC). Immunohistochemical analysis was used to determine the expression of MAGE-A9 in 201 cases CRC tissues. We used quantitative real-time polymerase chain reaction (RT-PCR) and western blot analysis to further verify the results. The correlation between MAGE-A9 expression, clinicopathological features and prognosis of CRC patients was analyzed. The results showed that MAGE-A9 was predominantly localized in the cytoplasm of cancer cells and stromal cells. Compared to normal adjacent tissues, the high expression rate of MAGE-A9 in CRC tissues was significantly increased (P<0.001). High MAGE-A9 expression was significantly associated with venous invasion (P=0.008) and lymph node metastasis (P<0.001). The survival rate of the CRC patients who were positive for MAGE-A9 expression was significantly lower than that of CRC patients with negative MAGE-A9 expression. Moreover, univariate and multivariate analyses showed that high MAGE-A9 expression was a poor prognostic factor for CRC patients. Hence, MAGE-A9 is expected to become a new target for CRC treatment.
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Lanzhou Petrochemical Hospital, Fuli West Road No. 487, Xigu District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Zhaoqin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Jichi
authors.initials: J
authors.lastname: Ma
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Yuanhui
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Gansu Provincial Hospital, Donggang West Road No. 204, Chengguan District, Lanzhou City 730030, Gansu Province, China. Electronic address: gansuyj66@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011379:Prognosis; D015996:Survival Rate
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C520253:MAGEA9 protein, human; D009363:Neoplasm Proteins