title: 15-Deoxy-prostaglandin J2 anti-inflammation in a rat model of chronic obstructive pulmonary disease and human bronchial epithelial cells via Nrf2 activation.
abstract: Nuclear factor-erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates antioxidant and anti-inflammatory genes, and it plays a crucial role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Moreover, 15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) plays a protective role against oxidative stress and inflammation both in vivo and in vitro. In a previous study, we found that 15d-PGJ2 increased the expression of Nrf2 in a COPD rat model. This study aims to elucidate the role of 15d-PGJ2 in COPD pathogenesis and the relationship between Nrf2 and human bronchial epithelial (HBE) cells. Normal HBE (HBE) cells were cultured. Following cigarette smoke extract (CSE) stimulation, pre-incubation with or without small interfering RNA (siRNA) Nrf2, and stimulation with or without 15d-PGJ2, the expression levels of Nrf2, NF-κBp65, and IL-8 were detected by reverse transcription-polymerase chain reaction and western blot, respectively. The expression of NF-κBp65 and IL-8 in CSE-stimulated normal HBE cells was inhibited by 15d-PGJ2 at both the mRNA level and the protein level. Moreover, the expression of Nrf2 in normal HBE cells was improved by 15d-PGJ2 at both the mRNA level and the protein level. However, the inhibitory or improving effects of 15d-PGJ2 were disengaged by siRNA Nrf2 at both the mRNA level and the protein level. 15d-PGJ2 possesses anti-inflammatory properties in the pathogenesis of COPD, and HBE cells stimulated by CSE via Nrf2 activation.
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, Hunan, China bllo181@163.com.
authors.forename: B L
authors.initials: BL
authors.lastname: Luo
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, Hunan People's Hospital, Changsha, Hunan, China.
authors.forename: W D
authors.initials: WD
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, Hunan People's Hospital, Changsha, Hunan, China.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D051267:NF-E2-Related Factor 2 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism
chemical_list: C477819:15-deoxyprostaglandin J2; D000893:Anti-Inflammatory Agents; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C495637:Nfe2l2 protein, rat; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D015230:Prostaglandin D2