title: Analysis of the TCP genes expressed in the inflorescence of the orchid Orchis italica.
abstract: TCP proteins are plant-specific transcription factors involved in many different processes. Because of their involvement in a large number of developmental pathways, their roles have been investigated in various plant species. However, there are almost no studies of this transcription factor family in orchids. Based on the available transcriptome of the inflorescence of the orchid Orchis italica, in the present study we identified 12 transcripts encoding TCP proteins. The phylogenetic analysis showed that they belong to different TCP classes (I and II) and groups (PCF, CIN and CYC/TB1), and that they display a number of conserved motifs when compared with the TCPs of Arabidopsis and Oryza. The presence of a specific cleavage site for the microRNA miRNA319, an important post-transcriptional regulator of several TCP genes in other species, was demonstrated for one transcript of O. italica, and the analysis of the expression pattern of the TCP transcripts in different inflorescence organs and in leaf tissue suggests that some TCP transcripts of O. italica exert their role only in specific tissues, while others may play multiple roles in different tissues. In addition, the evolutionary analysis showed a general purifying selection acting on the coding region of these transcripts.
authors.affiliation: Department of Biology, University of Naples Federico II, Napoli, Italy.
authors.forename: Sofia
authors.initials: S
authors.lastname: De Paolo
authors.affiliation: Department of Biology, University of Naples Federico II, Napoli, Italy.
authors.forename: Luciano
authors.initials: L
authors.lastname: Gaudio
authors.affiliation: Department of Biology, University of Naples Federico II, Napoli, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Aceto
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D019143:Evolution, Molecular; D056626:Inflorescence / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000737:chemistry / Q000378:metabolism; D029595:Orchidaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D014157:Transcription Factors