title: Staphylococcus-Candida Interaction Models: Antibiotic Resistance Testing and Host Interactions.
abstract: The fungus Candida albicans and bacterium Staphylococcus aureus can coexist in polymicrobial biofilms. S. aureus attaches strongly to hyphae, but not to the yeast form, of C. albicans with important consequences for virulence. Hyphae-associated S. aureus is less susceptible to antibiotic treatment. Furthermore, co-inoculation of C. albicans and S. aureus causes more severe and widespread infection than either microorganism alone. In this chapter, a basic in vitro model for studying the interaction between C. albicans hyphae and S. aureus is presented, which makes use of a fluorescently labeled S. aureus strain. Furthermore, two protocols are described that allow investigation of the effect of C. albicans and S. aureus interaction on antibiotic susceptibility or on interactions with the host. The latter focuses on phagocytosis of C. albicans-adhered S. aureus by macrophages. The protocols presented here may serve as a starting point to study the interaction of C. albicans with various other bacterial species.
authors.affiliation: Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.
authors.forename: Nina
authors.initials: N
authors.lastname: Scheres
authors.affiliation: Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Free University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands. b.krom@acta.nl.
authors.forename: Bastiaan P
authors.initials: BP
authors.lastname: Krom
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D002460:Cell Line; D060085:Coinfection; D004352:Drug Resistance, Microbial; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D051379:Mice; D056265:Microbial Interactions; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D010587:Phagocytosis; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*; D059008:Time-Lapse Imaging
keywords: Antibiotic treatment; Candida albicans; Fungus; Macrophages; Staphylococcus aureus