title: Human eukaryotic elongation factor 1A forms oligomers through specific cysteine residues.
abstract: Eukaryotic elongation factor 1A (eEF1A) is a multifunctional protein involved in bundling actin, severing microtubule, activating the phosphoinositol-4 kinase, and recruiting aminoacyl-tRNAs to ribosomes during protein biosynthesis. Although evidence has shown the presence of the isoform eEF1A1 oligomers, the substantial mechanism of the self-association remains unclear. Herein, we found that human eEF1A1 could spontaneously form oligomers. Specifically, mutagenesis screen on cysteine residues demonstrated that Cys(234) was essential for eEF1A1 oligomerization. In addition, we also found that hydrogen peroxide treatment could induce the formation of eEF1A oligomers in cells. By cysteine replacement, eEF1A2 isoform displayed the ability to oligomerize in cells under the oxidative environment. In summary, in this study we characterized eEF1A1 oligomerization and demonstrated that specific cysteine residues are required for this oligomerization activity.
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Guangshi
authors.initials: G
authors.lastname: Du
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Muran
authors.initials: M
authors.lastname: Ding
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Life Sciences and Key Laboratory of Bio-Resources and Eco-Environment, Sichuan University, Ministry of Education, Chengdu 610064, China wuchuanfangsichuan@gmail.com.
authors.forename: Chuanfang
authors.initials: C
authors.lastname: Wu
mesh_terms: D000199:Actins / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D016296:Mutagenesis; D009154:Mutation; D016366:Open Reading Frames; D018384:Oxidative Stress; D020648:Peptide Elongation Factor 1 / Q000737:chemistry*; D010766:Phosphorylation; D011485:Protein Binding; D014176:Protein Biosynthesis; D020033:Protein Isoforms / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D000199:Actins; C094814:EEF1A1 protein, human; D020648:Peptide Elongation Factor 1; D020033:Protein Isoforms; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D003545:Cysteine
keywords: cysteine; eEF1A1; oligomerization