title: Bioremediation strategies of hydrocarbons and microbial diversity in the Trindade Island shoreline--Brazil.
abstract: This study analyzed the microbial diversity colonizing the surface of an oil sample during its contact with water, off the Trindade Island coast and simulated the efficiency of eight different bioremediation strategies for this environment. The diversity analysis was performed using acrylic coupons that served as the support for an oil inclusion at sea. The coupons were sampled over 30 days, and T-RFLP multiplex was employed to access the diversity of fungi, Bacteria and Archaea present on the oil surface. The bioremediation strategies were simulated in a respirometer. The results showed that the bacterial domain was the most dominant in oil colonization and that the richness of the species attached to the oil gradually increases with the exposure time of the coupons. The combination of biostimulation and bioaugmentation with a native population was proven to be an effective strategy for the remediation of oil off the Trindade Island shoreline.
authors.affiliation: Laboratory of Environmental Biotechnology and Biodiversity, Microbiology Department Federal University of Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address: edmomontes@yahoo.com.br.
authors.forename: Edmo M
authors.initials: EM
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Environmental Biotechnology and Biodiversity, Microbiology Department Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Karlos H M
authors.initials: KH
authors.lastname: Kalks
authors.affiliation: Laboratory of Environmental Biotechnology and Biodiversity, Microbiology Department Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Péricles L
authors.initials: PL
authors.lastname: Fernandes
authors.affiliation: Laboratory of Environmental Biotechnology and Biodiversity, Microbiology Department Federal University of Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address: totolaufv@gmail.com.
authors.forename: Marcos R
authors.initials: MR
authors.lastname: Tótola
mesh_terms: D001105:Archaea / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D001938:Brazil; D006838:Hydrocarbons / Q000378:metabolism; D062312:Islands; D059013:Microbial Consortia / Q000502:physiology; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution; D012240:Rhodococcus / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*
chemical_list: D006838:Hydrocarbons; D010578:Petroleum
keywords: Bioremediation; Hydrocarbon degradation; Ocean contamination; Pristine environment