title: Generation and Characterization of Smad7 Conditional Knockout Mice.
abstract: Smad7 is an important negative modulator that regulates the duration and strength of TGF-β signaling. Dysregulation of Smad7 is associated with the pathogenesis of many human diseases. Various mouse models are developed to facilitate addressing the physiological functions of Smad7. We have recently demonstrated that loss of Smad7 function by deletion in its MH2 domain leads to multiple cardiac defects and aggravates ethanol-induced liver injury. Here, we describe the procedure to construct and characterize the Smad7 conditional knockout mice.
authors.affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. ychen3@sibs.ac.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial; D053595:Embryonic Stem Cells / Q000378:metabolism; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D051906:Smad7 Protein / Q000172:deficiency / Q000235:genetics*
chemical_list: D051906:Smad7 Protein
keywords: Gene deletion; Heart development; Mouse model; Smad7