title: Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer.
abstract: Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.
authors.affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.|School of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: Shi-Jie
authors.initials: SJ
authors.lastname: Li
authors.affiliation: School of Science, Shantou University, Shantou, China.
authors.forename: Tong-Song
authors.initials: TS
authors.lastname: Wang
authors.affiliation: School of Life Science, Xiamen University, Xiamen, China.
authors.forename: Fu-Niu
authors.initials: FN
authors.lastname: Qin
authors.affiliation: College of Life Science, Anqing Normal University, Anqing, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Liang
authors.affiliation: School of Science, Shantou University, Shantou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Song
authors.affiliation: Colleage of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Zeng-Ming
authors.initials: ZM
authors.lastname: Yang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D010064:Embryo Implantation / Q000502:physiology*; D004624:Embryo Transfer / Q000379:methods*; D047108:Embryonic Development / Q000502:physiology; D004717:Endometrium / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D011555:Pseudopregnancy / Q000196:embryology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism; D015053:Zygote / Q000254:growth & development / Q000637:transplantation*
chemical_list: D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2