title: Phytochrome A and B Function Antagonistically to Regulate Cold Tolerance via Abscisic Acid-Dependent Jasmonate Signaling.
abstract: Light signaling and phytohormones both influence plant growth, development, and stress responses; however, cross talk between these two signaling pathways in response to cold remains underexplored. Here, we report that far-red light (FR) and red light (R) perceived by phytochrome A (phyA) and phyB positively and negatively regulated cold tolerance, respectively, in tomato (Solanum lycopersicum), which were associated with the regulation of levels of phytohormones such as abscisic acid (ABA) and jasmonic acid (JA) and transcript levels of ABA- and JA-related genes and the C-REPEAT BINDING FACTOR (CBF) stress signaling pathway genes. A reduction in the R/FR ratio did not alter cold tolerance, ABA and JA accumulation, and transcript levels of ABA- and JA-related genes and the CBF pathway genes in phyA mutant plants; however, those were significantly increased in wild-type and phyB plants with the reduction in the R/FR ratio. Even though low R/FR treatments did not confer cold tolerance in ABA-deficient (notabilis [not]) and JA-deficient (prosystemin-mediated responses2 [spr2]) mutants, it up-regulated ABA accumulation and signaling in the spr2 mutant, with no effect on JA levels and signaling in the not mutant. Foliar application of ABA and JA further confirmed that JA functioned downstream of ABA to activate the CBF pathway in light quality-mediated cold tolerance. It is concluded that phyA and phyB function antagonistically to regulate cold tolerance that essentially involves FR light-induced activation of phyA to induce ABA signaling and, subsequently, JA signaling, leading to an activation of the CBF pathway and a cold response in tomato plants.
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0001-5351-1531
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Huizi
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Eugen
authors.identifier: http://orcid.org/0000-0001-7292-9802
authors.initials: E
authors.lastname: Onac
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Kai
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.).
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, People's Republic of China (F.W., Z.G., H.L., J.Z., X.X., K.S., J.Y., Y.Z.);Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, People's Republic of China (F.W., J.Y., Y.Z.);Philips Research China, Shanghai 200233, People's Republic of China (M.W.); andPhilips Research Europe, High Tech Campus 34, 5656 AE Eindhoven, The Netherlands (E.O.) yanhongzhou@zju.edu.cn.
authors.forename: Yanhong
authors.identifier: http://orcid.org/0000-0002-7860-8847
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D003080:Cold Temperature; D003517:Cyclopentanes / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D008027:Light; D018551:Solanum lycopersicum / Q000187:drug effects / Q000502:physiology*; D009154:Mutation; D054883:Oxylipins / Q000378:metabolism* / Q000494:pharmacology; D051942:Phytochrome A / Q000235:genetics / Q000378:metabolism*; D051939:Phytochrome B / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D051942:Phytochrome A; D051939:Phytochrome B; C011006:jasmonic acid; D000040:Abscisic Acid