title: Protective role of carbon dioxide (CO2) in generation of reactive oxygen species.
abstract: The results testify to the fact that CO2 is a powerful inhibitor of reactive oxygen species (ROS) generation by cells (blood phagocytes and alveolar macrophages of 96 people and cells of inner organs and tissue phagocytes (of liver, brain, myocardium, lungs, kidneys, stomach, and skeleton muscles), as well as by mitochondria of the liver of 186 white mice and human tissues. Generation of ROS was determined using various methods with CO2 directly acting on the cells and bioptates and indirectly on the organism as a whole. CO2 in the concentration of 5.1% (P = 37.5 mmHg), 8.2% (P = 60.0 mmHg), and 20% (P = 146.0 mmHg) in a mixture with air (total pressure = 730 mmHg) inhibits the basal ROS generation by phagocytes on the average by 3.52, 5.69, and 10.03 times, respectively (p < 0.05), and the stimulated by corpuscular particles: (a) zymosan by 3.24, 4.43, and 7.95 times; (b)SiO2: by 2.99, 3.24, and 5.76 times (p < 0.05). This is confirmed by the feet that CO2, along with inhibiting the O2 (-) generation by cells of the various organs, including the liver, as a rule, by 2.19-4.7 times, p < 0.01 or <0.001 induces simultaneously a decrease in the O2 (-) generation by mitochondria isolated from the liver (by 1.91-3.2 times, p < 0.001). The mechanism of CO2 influence is realized, in part, by inhibition of NADPH-oxidase activity. Taken into consideration proven role of CO2 in different pathophysiological conditions, (such as endoarteritis, bronchial asthma, and infectious diseases), present findings may be of clinical interest in terms of potential implementation of CO2 donors as adjuvant therapeutics in these diseases.
authors.affiliation: Department of Human Pathology, 1st Moscow State Medical University IM Sechenov, Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Bolevich
authors.affiliation: Department of Human Pathology, 1st Moscow State Medical University IM Sechenov, Moscow, Russia.
authors.forename: Alekandr Haritonovic
authors.initials: AH
authors.lastname: Kogan
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zivkovic
authors.affiliation: Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
authors.forename: Dusan
authors.initials: D
authors.lastname: Djuric
authors.affiliation: Department of Human Pathology, 1st Moscow State Medical University IM Sechenov, Moscow, Russia.
authors.forename: Aleksey Aleksejevic
authors.initials: AA
authors.lastname: Novikov
authors.affiliation: Department of Human Pathology, 1st Moscow State Medical University IM Sechenov, Moscow, Russia.
authors.forename: Sergey Ivanovic
authors.initials: SI
authors.lastname: Vorobyev
authors.affiliation: Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia. drvladakgbg@yahoo.com.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Jakovljevic
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism*; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D002245:Carbon Dioxide
keywords: Animals; Carbon dioxide; Humans; Reactive oxygen species