title: Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity.
abstract: BACKGROUND
  The aim of this study was to develop chick embryo chorioallantoic membrane (CAM) model of laryngeal squamous cell carcinoma (LSCC) and to evaluate the morphological and morphometric characteristics and angiogenic features of it.
  
  
  METHODS
  Fresh LSCC tissue samples obtained from 6 patients were implanted onto 15 chick embryo CAMs. Morphological, morphometric, and angiogenic changes in the CAM and chorionic epithelium were evaluated up to 4 days after the tumor implantation. Immunohistochemical analysis (34βE12, CD31, and Ki67 staining) was performed to detect cytokeratins and tumor endothelial cells and to evaluate the proliferative capacity of the tumor before and after implantation on the CAM.
  
  
  RESULTS
  The implanted LSCC tissue samples survived on the CAM in all the experiments and retained the essential morphologic characteristics and proliferative capacity of the original tumor. Implants induced thickening of both the CAM (103-417%, p = 0.0001) and the chorionic epithelium (70-140%, p = 0.0001) and increase in number of blood vessels (75-148%, p = 0.0001) in the CAM.
  
  
  CONCLUSIONS
  This study clarifies that chick embryo CAM is a relevant assay for implanting LSCC tissue and provides the first morphological and morphometric characterization of the LSCC CAM model that opens new perspectives to study this disease.
authors.affiliation: Department of Otorhinolaryngology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Virgilijus
authors.initials: V
authors.lastname: Uloza
authors.affiliation: Department of Otorhinolaryngology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Alina
authors.initials: A
authors.lastname: Kuzminienė
authors.affiliation: Department of Histology and Embryology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Sonata
authors.initials: S
authors.lastname: Šalomskaitė-Davalgienė
authors.affiliation: Department of Histology and Embryology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Jolita
authors.initials: J
authors.lastname: Palubinskienė
authors.affiliation: Department of Histology and Embryology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Ingrida
authors.initials: I
authors.lastname: Balnytė
authors.affiliation: Department of Otorhinolaryngology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Ingrida
authors.initials: I
authors.lastname: Ulozienė
authors.affiliation: Department of Physics, Mathematics and Biophysics, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Viktoras
authors.initials: V
authors.lastname: Šaferis
authors.affiliation: Department of Histology and Embryology, Lithuanian University of Health Sciences, LT-50009 Kaunas, Lithuania.
authors.forename: Angelija
authors.initials: A
authors.lastname: Valančiūtė
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000254:growth & development* / Q000473:pathology; D002823:Chorion / Q000473:pathology; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D009389:Neovascularization, Pathologic