title: [Expression of CSN Complex in ATRA-induced APL Cell Differentiation and Its Clinical Significance].
abstract: OBJECTIVE
  To investigate the expression of CSN complex (COP9 signal some subunits) in the patients with acute promyelocytic leukemia (APL) and its significance in the ATRA-induced APL differentiation.
  
  
  METHODS
  Using the NB4 cells as a model, morphologic observation and myeloid differentiation marker CD11b detection were used to monitor ATRA-induced APL differentiation, the expression of CSN complex in cell differentiation was detected by Western blot and reverse transcription real time fluorescent quantitative PCR (RT-qPCR) method. RT-qPCR was also used to detect the relative expression level of COP9 signalosome subunits in the APL patients and remission after treatment.
  
  
  RESULTS
  ATRA could obviously enhance CD11b expression; the cell morphology showed obvious differentiation characteristics. During the differentiation, the expression of COP9 signalosome subunits was down-regulated by ATRA. Meanwhile, the CSN expression level in newly diagnosed APL patients was much higher than that in controls (non-leukemia) (P < 0.05). The level of CSN expression was obviously down-regulated when APL patients achieved complete remission.
  
  
  CONCLUSIONS
  The high CSN expression level in APL patients can be down-regulated by ATRA. CSN complex may have a significant effect on the pathogenesis and therapy of APL.
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Shu-Yuan
authors.initials: SY
authors.lastname: Liu
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: La-Gen
authors.initials: LG
authors.lastname: Wan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Gao
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Yun-Yuan
authors.initials: YY
authors.lastname: Kong
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Laboratorial Examination, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. E-mail: zhzl1984@sohu.com.
authors.forename: Zhang-Lin
authors.initials: ZL
authors.lastname: Zhang
mesh_terms: D000075686:COP9 Signalosome Complex; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D015536:Down-Regulation; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D046912:Multiprotein Complexes; D014212:Tretinoin; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex