title: Bacillus cereus eﬄux protein BC3310 - a multidrug transporter of the unknown major facilitator family, UMF-2.
abstract: Phylogenetic classification divides the major facilitator superfamily (MFS) into 82 families, including 25 families that are comprised of transporters with no characterized functions. This study describes functional data for BC3310 from Bacillus cereus ATCC 14579, a member of the "unknown major facilitator family-2" (UMF-2). BC3310 was shown to be a multidrug eﬄux pump conferring resistance to ethidium bromide, SDS and silver nitrate when heterologously expressed in Escherichia coli DH5α ΔacrAB. A conserved aspartate residue (D105) in putative transmembrane helix 4 was identified, which was essential for the energy dependent ethidium bromide eﬄux by BC3310. Transport proteins of the MFS comprise specific sequence motifs. Sequence analysis of UMF-2 proteins revealed that they carry a variant of the MFS motif A, which may be used as a marker to distinguish easily between this family and other MFS proteins. Genes orthologous to bc3310 are highly conserved within the B. cereus group of organisms and thus belong to the core genome, suggesting an important conserved functional role in the normal physiology of these bacteria.
authors.affiliation: Laboratory for Microbial Dynamics, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo Oslo, Norway ; Institut für Pharmazeutische Biologie und Biotechnologie, Albert-Ludwigs Universität Freiburg, Germany.
authors.forename: Jasmin K
authors.initials: JK
authors.lastname: Kroeger
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney NSW, Australia.
authors.forename: Karl
authors.initials: K
authors.lastname: Hassan
authors.affiliation: Laboratory for Microbial Dynamics, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo Oslo, Norway.
authors.forename: Aniko
authors.initials: A
authors.lastname: Vörös
authors.affiliation: Laboratory for Microbial Dynamics, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo Oslo, Norway.
authors.forename: Roger
authors.initials: R
authors.lastname: Simm
authors.affiliation: School of BioMedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds Leeds, UK.
authors.forename: Massoud
authors.initials: M
authors.lastname: Saidijam
authors.affiliation: School of BioMedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds Leeds, UK.
authors.forename: Kim E
authors.initials: KE
authors.lastname: Bettaney
authors.affiliation: Institut für Pharmazeutische Biologie und Biotechnologie, Albert-Ludwigs Universität Freiburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
authors.affiliation: Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney NSW, Australia.
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
authors.affiliation: School of BioMedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds Leeds, UK.
authors.forename: Peter J F
authors.initials: PJ
authors.lastname: Henderson
authors.affiliation: Laboratory for Microbial Dynamics, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo Oslo, Norway.
authors.forename: Anne-Brit
authors.initials: AB
authors.lastname: Kolstø
keywords: Bacillus cereus; MFS; UMF-2; drug resistance; eﬄux protein