title: Effects of Controlled Cortical Impact on the Mouse Brain Vasculome.
abstract: Perturbations in blood vessels play a critical role in the pathophysiology of brain injury and neurodegeneration. Here, we use a systematic genome-wide transcriptome screening approach to investigate the vasculome after brain trauma in mice. Mice were subjected to controlled cortical impact and brains were extracted for analysis at 24 h post-injury. The core of the traumatic lesion was removed and then cortical microvesels were isolated from nondirectly damaged ipsilateral cortex. Compared to contralateral cortex and normal cortex from sham-operated mice, we identified a wide spectrum of responses in the vasculome after trauma. Up-regulated pathways included those involved in regulation of inflammation and extracellular matrix processes. Decreased pathways included those involved in regulation of metabolism, mitochondrial function, and transport systems. These findings suggest that microvascular perturbations can be widespread and not necessarily localized to core areas of direct injury per se and may further provide a broader gene network context for existing knowledge regarding inflammation, metabolism, and blood-brain barrier alterations after brain trauma. Further efforts are warranted to map the vasculome with higher spatial and temporal resolution from acute to delayed phase post-trauma. Investigating the widespread network responses in the vasculome may reveal potential mechanisms, therapeutic targets, and biomarkers for traumatic brain injury.
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Guo
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.|2 Department of Pediatrics, Massachusetts General Hospital , Harvard Medical School, Boston, Massachusetts.
authors.forename: Josephine
authors.initials: J
authors.lastname: Lok
authors.affiliation: 3 The Department of Spine Surgery, the First Hospital of Jilin University , Changchun, China .
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Wendy
authors.initials: W
authors.lastname: Leung
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Angel T
authors.initials: AT
authors.lastname: Som
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Hayakawa
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Qingzhi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Changhong
authors.initials: C
authors.lastname: Xing
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.affiliation: 4 Cerebrovascular Research Center, Department of Neurosurgery, Xuanwu Hospital, Capital Medical University , Beijing, China .
authors.forename: Xunming
authors.initials: X
authors.lastname: Ji
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: 1 Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital , Harvard Medical School, Charlestown, Massachusetts.
authors.forename: Eng H
authors.initials: EH
authors.lastname: Lo
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000276:immunology / Q000378:metabolism; D000070642:Brain Injuries, Traumatic / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000293:injuries*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055806:Microvessels / Q000276:immunology / Q000378:metabolism; D059467:Transcriptome
keywords: extracellular matrix; inflammation; microvessels; neurovascular unit; traumatic brain injury