title: Green fabricated reduced graphene oxide: evaluation of its application as nano-carrier for pH-sensitive drug delivery.
abstract: A green and mild approach for the preparation of reduced graphene oxide (rGO) was proposed by using riboflavin-5'-phosphate sodium salt dihydrate as a reducing reagent and stabilizer without any other reagent. The fabricated nano-rGO was systematically evaluated for its application as nano-carrier for pH-sensitive drug delivery. The hemolytic toxicity test indicated the as-prepared nano-rGO had negligible hemolytic activity, which demonstrating its safety in drug delivery system. Doxorubicin hydrochloride (DOX) as a model drug was successfully attached onto the surface of nano-rGO via strong π-π stacking interaction. Compared with common carriers, the obtained DOX-loaded nano-rGO nanohybrid exhibited characteristics of high drug loading content, good stability, pH-sensitive and sustainable release of drugs. Cytotoxicity assay results suggested such nanohybrid exhibited effective cytotoxicity to MCF-7 and A549 cells by nonspecific endocytosis mechanism. Therefore, the present green fabricated rGO could be a good candidate as an ideal nano-carrier for drug delivery and controlled release.
authors.affiliation: School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan, Shandong Province, 250012, PR China.
authors.forename: Naxin
authors.initials: N
authors.lastname: Ma
authors.affiliation: Key Lab of Colloid & Interface Chemistry, Shandong University, Ministry of Education, 250100, PR China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan, Shandong Province, 250012, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan, Shandong Province, 250012, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Lab of Colloid & Interface Chemistry, Shandong University, Ministry of Education, 250100, PR China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan, Shandong Province, 250012, PR China. Electronic address: yuxialuan@sdu.edu.cn.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Luan
mesh_terms: D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000008:administration & dosage; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004337:Drug Carriers; D010087:Oxides; D006108:Graphite; D004317:Doxorubicin
keywords: Drug carrier; Graphene oxide; Green chemistry; Reduced graphene oxide