title: A set of GFP-based organelle marker lines combined with DsRed-based gateway vectors for subcellular localization study in rice (Oryza sativa L.).
abstract: In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.|Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC.
authors.forename: Tsung-Meng
authors.initials: TM
authors.lastname: Wu
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.
authors.forename: Ke-Chun
authors.initials: KC
authors.lastname: Lin
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.
authors.forename: Wei-Shiang
authors.initials: WS
authors.lastname: Liau
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.|Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC.
authors.forename: Yun-Yang
authors.initials: YY
authors.lastname: Chao
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.
authors.forename: Ling-Hung
authors.initials: LH
authors.lastname: Yang
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC.
authors.forename: Szu-Yun
authors.initials: SY
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, National Central University, Jhongli City, Taoyuan County, 320, Taiwan, ROC.
authors.forename: Chung-An
authors.initials: CA
authors.lastname: Lu
authors.affiliation: Department of Agricultural Chemistry, College of Bioresources and Agriculture, National Taiwan University, Taipei, 10617, Taiwan, ROC. cyhong@ntu.edu.tw.
authors.forename: Chwan-Yang
authors.initials: CY
authors.lastname: Hong
mesh_terms: D060387:Ascorbate Peroxidases / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011523:Protoplasts; D011993:Recombinant Fusion Proteins
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins; D060387:Ascorbate Peroxidases
keywords: DsRed; GFP; Organelle marker lines; Oryza sativa; Protoplast; Subcellular localization