title: Crystallization and X-ray diffraction studies of a complete bacterial fatty-acid synthase type I.
abstract: While a deep understanding of the fungal and mammalian multi-enzyme type I fatty-acid synthases (FAS I) has been achieved in recent years, the bacterial FAS I family, which is narrowly distributed within the Actinomycetales genera Mycobacterium, Corynebacterium and Nocardia, is still poorly understood. This is of particular relevance for two reasons: (i) although homologous to fungal FAS I, cryo-electron microscopic studies have shown that bacterial FAS I has unique structural and functional properties, and (ii) M. tuberculosis FAS I is a drug target for the therapeutic treatment of tuberculosis (TB) and therefore is of extraordinary importance as a drug target. Crystals of FAS I from C. efficiens, a homologue of M. tuberculosis FAS I, were produced and diffracted X-rays to about 4.5 Å resolution.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main, Germany.
authors.forename: Mathias
authors.initials: M
authors.lastname: Enderle
authors.affiliation: EMBL Grenoble, 71 Avenue des Martyrs, 38042 Grenoble CEDEX 9, France.
authors.forename: Andrew
authors.initials: A
authors.lastname: McCarthy
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main, Germany.
authors.forename: Karthik Shivaji
authors.initials: KS
authors.lastname: Paithankar
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Grininger
mesh_terms: D003352:Corynebacterium / Q000201:enzymology*; D003460:Crystallization; D054890:Fatty Acid Synthase, Type I / Q000737:chemistry* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D014961:X-Ray Diffraction
chemical_list: D054890:Fatty Acid Synthase, Type I
keywords: fatty-acid synthase; fatty-acid synthesis; multienzyme; mycolic acid; tuberculosis