title: The activity of detoxifying enzymes in the infective juveniles of Heterorhabditis bacteriophora strains: Purification and characterization of two acetylcholinesterases.
abstract: The infectivity and detoxifying enzyme activities including glutathione-S-transferase (GST), acetylcholinesterase (AChE) and carboxylesterase (CaE) are investigated in the infective juveniles (IJs) of six different strains of Heterorhabditis bacteriophora as a biocontrol agent against insect pests. The specific activities ranged from 10.8-29.8 and 50-220units/mg protein for GST and AChE, respectively; and from 24.7-129 and 22.6-77.3units/mg protein for CaE as estimated by P-nitrophenyl and α-naphthyl acetates, respectively. H. bacteriophora EM2 strain has the highest infectivity and the highest enzymatic activities as well. AChE is the predominant detoxifying enzyme that might imply its major role in the detoxification of insecticide(s). The isoenzyme pattern demonstrated two major slow-moving isoforms in all EPN strains examined. Purification of two AChE isoforms, AChEAII and AChEBI, from H. bacteriophora EM2 strain is performed by ammonium sulfate precipitation, gel filtration on Sephacryl S-200 and chromatography on DEAE-Sepharose. AChEAII and AChEBII have specific activities of 1207 and 1560unit/mg protein, native molecular weights of 180 and 68kDa, and are found in dimeric and monomeric forms, respectively. Both isoforms showed optimum activity at pH8.5 and 35°C. AChEBI exhibited higher thermal stability and higher activation energy than AChEAII. The enzymatic activities of purified AChEs are completely inhibited by Hg(+2) and Ni(+2) and greatly enhanced by Mn(+2). The substrate specificity, the relative efficiency of substrates hydrolysis, substrate inhibition and inhibition by BW284C51, but not by iso-OMPA, clearly indicated that they are true AChEs; their properties are compared with those recorded for insects as target hosts for H. bacteriophora EM2.
authors.affiliation: Molecular Biology Department, National Research Centre, 33-El Bohouth St. Dokki, P.O. 12622, Giza, Egypt. Electronic address: magdasoroor@yahoo.com.
authors.forename: Magda A
authors.initials: MA
authors.lastname: Mohamed
authors.affiliation: Biochemistry Department, Hellwan University, Cairo, Egypt.
authors.forename: El-Sayed M E
authors.initials: el-SM
authors.lastname: Mahdy
authors.affiliation: Molecular Biology Department, National Research Centre, 33-El Bohouth St. Dokki, P.O. 12622, Giza, Egypt.
authors.forename: Abd-El-Hady M
authors.initials: AE
authors.lastname: Ghazy
authors.affiliation: Molecular Biology Department, National Research Centre, 33-El Bohouth St. Dokki, P.O. 12622, Giza, Egypt.
authors.forename: Nihal M
authors.initials: NM
authors.lastname: Ibrahim
authors.affiliation: Biochemistry Department, Hellwan University, Cairo, Egypt.
authors.forename: Hatem A
authors.initials: HA
authors.lastname: El-Mezayen
authors.affiliation: Molecular Biology Department, National Research Centre, 33-El Bohouth St. Dokki, P.O. 12622, Giza, Egypt.
authors.forename: Manal M E
authors.initials: MM
authors.lastname: Ghanem
mesh_terms: D000110:Acetylcholinesterase / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002412:Cations; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D006790:Host-Parasite Interactions; D007527:Isoenzymes; D008670:Metals; D009036:Moths / Q000469:parasitology; D009348:Nematoda / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D002412:Cations; D007527:Isoenzymes; D008670:Metals; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase (AChE); Characterization; Detoxifying enzymes; Entomopathogenic nematodes; Heterorhabditis bacteriophora; Inhibition; Kinetics; Purification