title: Hydrolyzed fish proteins reduced activation of caspase-3 in H2O2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar).
abstract: Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids and low molecular peptides that potentially benefit health. The following study aimed to test whether the water soluble phase of H-pro could reduce apoptosis and inflammation in primary liver cells isolated from Atlantic salmon following H2O2 provoked oxidative stress. Cells were grown as monocultures or co-cultured with head kidney cells to assess possible cross talk in inflammation and metabolism during treatments. Cells were grown in media with or without H-pro for 2 days before being stressed with 200 µM H2O2 then harvested 24 h post exposure. Both treatments were compared to the respective treatments without H2O2 supplementation. Oxidative stressed cells had increased activation of caspase-3, but supplementation with H-pro in the media prior to the oxidative stress reduced caspase-3 activation. In conclusion, free amino acids and low molecular weight peptides from H-pro attenuated oxidative stress, and made cells able to withstand apoptosis after H2O2 provoked oxidative stress.
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Espe
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Holen
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway ; Fish Nutrition Laboratory, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China.
authors.forename: J
authors.initials: J
authors.lastname: He
authors.affiliation: International Research Institute of Stavanger AS (IRIS), PoBox 8046, 4068 Stavanger, Norway.
authors.forename: F
authors.initials: F
authors.lastname: Provan
authors.affiliation: National Institute of Nutrition and Seafood Research (NIFES), PoBox 2029, 5817 Nordnes, Norway ; School of Life Science, East China Normal University, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: International Research Institute of Stavanger AS (IRIS), PoBox 8046, 4068 Stavanger, Norway.
authors.forename: K B
authors.initials: KB
authors.lastname: Øysæd
authors.affiliation: Hordafor AS, Salthella, 5397 Bekkjarvik, Norway.
authors.forename: J
authors.initials: J
authors.lastname: Seliussen
keywords: Atlantic salmon; Caspase-3; Co-cultures; Head kidney cells; Hydrolyzed proteins; Oxidative stress; Primary liver cells; Viability