title: Protein immobilization on the surface of polydimethylsiloxane and polymethyl methacrylate microfluidic devices.
abstract: PDMS and PMMA are two of the most used polymers in the fabrication of lab-on-chip or microfluidic devices. In order to use these polymers in biological applications, it is sometimes essential to be able to bind biomolecules such as proteins and DNA to the surface of these materials. In this work, we have evaluated a number of processes that have been developed to bind protein to PDMS surfaces which include passive adsorption, passive adsorption with glutaraldehyde cross-linking, (3-aminopropyl) triethoxysilane functionalization followed by glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride cross-linkers. It has been shown that the latter technique--using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride--results in more than twice the bonding of protein to the surface of PDMS microchannels than proteins binding passively. We have also evaluated a few techniques that have been tested for the functionalization of PMMA microchannels where we have found that the use of polyethyleneimine (PEI) has led to the strongest protein-PMMA microchannel bond. We finally demonstrated the effect of PDMS curing methodology on protein adsorption to its surface, and showed that increased curing time is the factor that reduces passive adsorption the most.
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Ruba
authors.initials: R
authors.lastname: Khnouf
authors.affiliation: Department of Biomedical Engineering, Faculty of Engineering, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Dina
authors.initials: D
authors.lastname: Karasneh
authors.affiliation: Department of Applied Physics, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Borhan Aldeen
authors.initials: BA
authors.lastname: Albiss
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D055767:Immobilized Proteins; C013830:baysilon; D019904:Polymethyl Methacrylate
keywords: Microfluidic devices; PDMS; PMMA; Protein immobilization; Surface modification