title: A specific antimicrobial protein CAP-1 from Pseudomonas sp. isolated from the jellyfish Cyanea capillata.
abstract: A bacterium strain, designated as CMF-2, was isolated from the jellyfish Cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. The strain CMF-2 was identified as Pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16S rRNA sequence analysis. In this study, an antimicrobial protein, named as CAP-1, was isolated from the culture of CMF-2 through ammonium sulfate precipitation and gel filtration chromatography. According to the result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), a major band indicated that the antimicrobial protein had a molecular mass of about 15 kDa, and it was identified as a hypothetical protein by MALDI-TOF-MS analysis and Mascot searching. CAP-1 displayed a broad antimicrobial spectrum against the indicator bacteria and fungus, including Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans, especially some marine-derived microorganisms such as Vibrio vulnificus, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio cholera, and Vibrio anguillarum, but showed little impact on tumor cells and normal human cells. The protein CAP-1 remained a stable antimicrobial activity in a wide range of temperature (20-80°C) and pH (2-10) conditions. These results suggested that CAP-1 might have a specific antimicrobial function not due to cytotoxicity.
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: mmy19900215@163.com.
authors.forename: Manman
authors.initials: M
authors.lastname: Yin
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: liudanzhongyao@163.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Food Microbiology, College of Food Science and Engineering, Dalian Ocean University, Dalian 116021, China. Electronic address: xufeng19880424@126.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: hormat830713@hotmail.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: abc_w@163.com.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: lilly_wang@126.com.
authors.forename: Beilei
authors.initials: B
authors.lastname: Wang
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China. Electronic address: donlone@yeah.net.
authors.forename: Yinlong
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: jmzheng@smmu.edu.cn.
authors.forename: Jiemin
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Tao
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: lgy_laurie@aliyun.com.
authors.forename: Guoyan
authors.initials: G
authors.lastname: Liu
authors.affiliation: Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai 200433, China; Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: lmzhang@smmu.edu.cn.
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D005285:Fermentation; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D037401:Scyphozoa / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013559:Symbiosis; D013696:Temperature
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S
keywords: Antimicrobial activity; Antimicrobial protein; Cyanea capillata