title: Channel formation by RTX-toxins of pathogenic bacteria: Basis of their biological activity.
abstract: The pore-forming cytolysins of the RTX-toxin (Repeats in ToXin) family are a relatively small fraction of a steadily increasing family of proteins that contain several functionally important glycine-rich and aspartate containing nonapeptide repeats. These cytolysins produced by a variety of Gram-negative bacteria form ion-permeable channels in erythrocytes and other eukaryotic cells. Hemolytic and cytolytic RTX-toxins represent pathogenicity factors of the toxin-producing bacteria and are very often important key factors in pathogenesis of the bacteria. Channel formation by RTX-toxins lead to the dissipation of ionic gradients and membrane potential across the cytoplasmic membrane of target cells, which results in cell death. Here we discuss channel formation and channel properties of some of the best known RTX-toxins, such as α-hemolysin (HlyA) of Escherichia coli and the uropathogenic EHEC strains, the adenylate cyclase toxin (ACT, CyaA) of Bordetella pertussis and the RTX-toxins (ApxI, ApxII and ApxIII) produced by different strains of Actinobacillus pleuropneumoniae. The channels formed by these RTX-toxins in lipid bilayers share some common properties such as cation selectivity and voltage-dependence. Furthermore the channels are transient and show frequent switching between different ion-conducting states. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale.
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University, Campus Ring 1, 28759, Bremen, Germany. Electronic address: r.benz@jacobs-university.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Benz
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001424:Bacterial Infections / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers / Q000378:metabolism; D052899:Pore Forming Cytotoxic Proteins / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D007473:Ion Channels; D008051:Lipid Bilayers; D052899:Pore Forming Cytotoxic Proteins
keywords: Electrophysiology; Hemolysin; Lipid bilayer; Pathogenicity factor; Pore formation; RTX-toxin