title: Clinical Significance of Contamination of the Preservation Solution in Liver Transplantation.
abstract: BACKGROUND
  The aim of the present study was to describe the incidence and microbiological profiles of positive cultures obtained from preservation solution (PS) and correlate these findings with infectious complications detected in the liver transplant (LT) recipient.
  
  
  METHODS
  We conducted a single-center, retrospective study between December 2010 and August 2014 among 178 LT. In all grafts, a PS culture was carried out. All the infections in the receipt until hospital discharge were collected. In patients with >1, infection was considered the most severe according to Clavien-Dindo classification.
  
  
  RESULTS
  PS culture was positive for bacterial or fungal agents in 79 of 178 LT recipients (44%). The most commonly cultured organisms were coagulase-negative staphylococci (64%), Enterobacteriaceae (17%), and Staphylococcus aureus (4.7%). In the 79 patients with positive PS, 49 blood cultures were requested in the period after LT. Twenty-five postoperative infections (31.7%) were diagnosed. Only 4 of 79 patients (5%) with PS contamination had a postoperative infections related with isolated microorganism.
  
  
  CONCLUSIONS
  Contamination of PS appears in a high percentage of liver grafts before LT, although there is a poor correlation with postoperative infections in LT recipient. In these patients, a standardized process including fungal and bacterial cultures could be useful.
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: C
authors.initials: C
authors.lastname: Garcia-Zamora
authors.affiliation: Department of Microbiology.
authors.forename: J
authors.initials: J
authors.lastname: Segura
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: V
authors.initials: V
authors.lastname: Lopez-Lopez
authors.affiliation: Department of Microbiology.
authors.forename: C
authors.initials: C
authors.lastname: Salvador
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: P A
authors.initials: PA
authors.lastname: Cascales Campos
authors.affiliation: Department of Hepatology.
authors.forename: J A
authors.initials: JA
authors.lastname: Pons Miñano
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: R
authors.initials: R
authors.lastname: Robles Campos
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: F
authors.initials: F
authors.lastname: Sanchez Bueno
authors.affiliation: Biologist Liver Transplant Unit.
authors.forename: R
authors.initials: R
authors.lastname: Gonzalez
authors.affiliation: Department of Microbiology.
authors.forename: G
authors.initials: G
authors.lastname: Yagüe
authors.affiliation: Liver Transplant Unit, Department of Surgery. Electronic address: ramirezp@um.es.
authors.forename: P
authors.initials: P
authors.lastname: Ramirez
authors.affiliation: Liver Transplant Unit, Department of Surgery.
authors.forename: P
authors.initials: P
authors.lastname: Parrilla Paricio
mesh_terms: D004340:Drug Contamination; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D019188:Organ Preservation Solutions; D012189:Retrospective Studies; D013210:Staphylococcus / Q000302:isolation & purification
chemical_list: D019188:Organ Preservation Solutions