title: Effects of Inhibitors of Necroptosis and Autophagy on Morphofunctional Characteristics of the Myocardium during Static Cold Storage of Donor Rat Heart.
abstract: Cardioprotective effects of necrostatin-1 (necroptosis inhibitor) and 3-methyladenine (autophagy inhibitor) were studied on the model of long-term cold preservation of rat heart. Addition of necrostatin-1 (490 nmol/liter) or 3-methyladenine (4.5 mmol/liter) to custodiol preserving solution reduced myocardial infarction size and improved left-ventricular function during reperfusion after 8-h preservation at 4°C. Inhibition of necroptosis and autophagy contributed to the cardioprotective effect under conditions of cold preservation of the donor heart.
authors.affiliation: North-Western Federal Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia. yuri.v.dmitriev@gmail.com.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Dmitriev
authors.affiliation: North-Western Federal Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: S M
authors.initials: SM
authors.lastname: Minasyan
authors.affiliation: North-Western Federal Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Vasina
authors.affiliation: North-Western Federal Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Demchenko
authors.affiliation: North-Western Federal Medical Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Galagudza
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002316:Cardiotonic Agents / Q000494:pharmacology; D003080:Cold Temperature; D015925:Cryopreservation; D005947:Glucose / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D009929:Organ Size; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods*
chemical_list: C039984:Bretschneider cardioplegic solution; D002316:Cardiotonic Agents; D007093:Imidazoles; D007211:Indoles; C507699:necrostatin-1; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose
keywords: 3-methyladenine; autophagy; cold preservation; necroptosis; necrostatin-1