title: Ex Vivo Signaling Protein Mapping in T Lymphocytes in the Psoriatic Arthritis Joints.
abstract: We assessed signaling protein mapping in total T cells, to analyze the proportions of T regulatory (Treg) and TCD4+ effector (Teff) cell phenotypes, and the respective interleukin 6Rα (IL-6Rα) expression in the inflammatory microenvironment of synovial fluid (SF) of patients with sustained psoriatic arthritis (PsA). Our approach was to measure the IL-6 level in SF using a multiplex bead immunoassay. Reverse-phase protein array was used to assess Janus kinase (JAK) 1 and JAK2, extra-cellular regulated kinase (ERK) 1 and 2, protein kinase Cδ (PKCδ), signal transducer and activator and transcription (STAT) 1, STAT3, and STAT5 phosphoproteins in total T cell lysates from SF of patients with PsA. Frequencies of CD4+IL-17A-F+IL-23+ CD4+ Th cells producing IL-17A and IL-17F (Th17) and CD4+CD25high intracellular forkhead box transcription factor+ (FOXP3+) phenotypes, and the percentage of Treg- and Teff- cells were quantified in SF and matched peripheral blood (PB) of patients with PsA and PB of healthy controls (HC) by flow cytometry. Our results were the following: In PsA SF samples, a coordinate increase of JAK1, ERK1/2, STAT1, STAT3, and STAT5 phosphoproteins was found in total T cells in SF of PsA; where IL-6 levels were higher than in PB from HC. Expanded CD4+IL-17A-F+IL-23+ Th17, CD4+ CD25- Teff- and CD4+CD25(high) FoxP3+Treg subsets, showing similar levels of enhanced IL-6Rδ expression, were confined to PsA joints. In our studies, the transcriptional network profile identified by ex vivo signaling protein mapping in T lymphocytes in PsA joints revealed the complex interplay between IL-1, IL-6, and IL-23 signaling and differentiation of Th17 cells and CD4+Tregs in sustained joint inflammation in PsA.
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Ugo
authors.initials: U
authors.lastname: Fiocco
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Veronica
authors.initials: V
authors.lastname: Martini
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Benedetta
authors.initials: B
authors.lastname: Accordi
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Francesco
authors.initials: F
authors.lastname: Caso
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Luisa
authors.initials: L
authors.lastname: Costa
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Francesca
authors.initials: F
authors.lastname: Oliviero
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Anna
authors.initials: A
authors.lastname: Scanu
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Mara
authors.initials: M
authors.lastname: Felicetti
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Paola
authors.initials: P
authors.lastname: Frallonardo
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Monica
authors.initials: M
authors.lastname: Facco
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Daniele
authors.initials: D
authors.lastname: Boso
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Beatrice
authors.initials: B
authors.lastname: Molena
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Renato
authors.initials: R
authors.lastname: Zambello
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Roberta
authors.initials: R
authors.lastname: Ramonda
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Franco
authors.initials: F
authors.lastname: Cozzi
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Raffaele
authors.initials: R
authors.lastname: Scarpa
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Basso
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Gianpietro
authors.initials: G
authors.lastname: Semenzato
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Dayer
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Andrea
authors.initials: A
authors.lastname: Doria
authors.affiliation: From the Rheumatology Unit, Department of Medicine DIMED, University of Padua; Hematology and Clinical Immunology Branch, University of Padua; Department of Woman and Child Health, University of Padua, Padua; Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II, Naples, Italy; and Faculty of Medicine, University Medical Center, University of Geneva, Geneva, Switzerland.U. Fiocco, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; V. Martini, Hematology and Clinical Immunology Branch, University of Padua; B. Accordi, Department of Woman and Child Health, University of Padua; F. Caso, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; L. Costa, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua and Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; F. Oliviero, Rheumatology Unit, Department of Medicine DIMED, University of Padua; A. Scanu, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Felicetti, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; P. Frallonardo, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; M. Facco, Hematology and Clinical Immunology Branch, University of Padua; D. Boso, MD, Hematology and Clinical Immunology Branch, University of Padua; B. Molena, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Zambello, MD, Hematology and Clinical Immunology Branch, University of Padua; R. Ramonda, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; F. Cozzi, MD, Rheumatology Unit, Department of Medicine DIMED, University of Padua; R. Scarpa, MD, Rheumatology Unit, Department of Clinical Medicine and Surgery, University Federico II; G. Basso, MD, Department of Woman and Child Health, University of Padua; G. Semenzato,
authors.forename: Leonardo
authors.initials: L
authors.lastname: Punzi
mesh_terms: D015535:Arthritis, Psoriatic / Q000201:enzymology* / Q000276:immunology; D016022:Case-Control Studies; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D015850:Interleukin-6 / Q000032:analysis; D007596:Joints / Q000201:enzymology* / Q000276:immunology; D010641:Phenotype; D010766:Phosphorylation; D040081:Protein Array Analysis; D060066:Protein Interaction Maps; D011494:Protein Kinases / Q000032:analysis*; D050791:STAT Transcription Factors / Q000032:analysis*; D015398:Signal Transduction; D013582:Synovial Fluid / Q000201:enzymology* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000201:enzymology* / Q000276:immunology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D050791:STAT Transcription Factors; D011494:Protein Kinases
keywords: INTERLEUKIN 6Rα SIGNALING; JAK1/STAT3/STAT5 PHOSPHOPROTEINS; PSORIATIC ARTHRITIS; T REGULATORY (TREG) CELLS; TH17 CELLS