title: A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria.
abstract: We present a method suitable for quantitative analysis of intracellular proteins, metabolites and secondary messengers of single bacterial cells. The method integrates the concept of immunoassays on a microfluidic device that facilitates single cell trapping and isolating in a small volume of a few tens of picoliters. Combination of the benefits of microfluidic systems for single cell analysis with the high analytical selectivity and sensitivity of immunoassays enables the detection of even low abundant intracellular analytes which occur only at a few hundred copies per bacterium.
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zurich, Vladimir-Prelog-Weg 3, Zurich, 8091, Switzerland.
authors.forename: Simone
authors.initials: S
authors.lastname: Stratz
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zurich, Vladimir-Prelog-Weg 3, Zurich, 8091, Switzerland. petra.dittrich@bsse.ethz.ch.
authors.forename: Petra S
authors.initials: PS
authors.lastname: Dittrich
mesh_terms: D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation*
chemical_list: D029968:Escherichia coli Proteins
keywords: Bacteria analysis; E. coli; Enzyme activity; Immunoassay; Microfluidics; Single-cell analysis