title: Identification of DNA lesions using a third base pair for amplification and nanopore sequencing.
abstract: Damage to the genome is implicated in the progression of cancer and stress-induced diseases. DNA lesions exist in low levels, and cannot be amplified by standard PCR because they are frequently strong blocks to polymerases. Here, we describe a method for PCR amplification of lesion-containing DNA in which the site and identity could be marked, copied and sequenced. Critical for this method is installation of either the dNaM or d5SICS nucleotides at the lesion site after processing via the base excision repair process. These marker nucleotides constitute an unnatural base pair, allowing large quantities of marked DNA to be made by PCR amplification. Sanger sequencing confirms the potential for this method to locate lesions by marking, amplifying and sequencing a lesion in the KRAS gene. Detection using the α-hemolysin nanopore is also developed to analyse the markers in individual DNA strands with the potential to identify multiple lesions per strand.
authors.affiliation: Department of Chemistry, University of Utah, 315S 1400 East, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Riedl
authors.affiliation: Department of Chemistry, University of Utah, 315S 1400 East, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Chemistry, University of Utah, 315S 1400 East, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Fleming
authors.affiliation: Department of Chemistry, University of Utah, 315S 1400 East, Salt Lake City, Utah 84112-0850, USA.
authors.forename: Cynthia J
authors.initials: CJ
authors.lastname: Burrows
mesh_terms: D020029:Base Pairing; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004249:DNA Damage / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D058608:Nanopores; D009711:Nucleotides / Q000032:analysis / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D013141:Spiro Compounds / Q000737:chemistry; D014498:Uracil / Q000737:chemistry; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D009711:Nucleotides; D013141:Spiro Compounds; D042461:Vascular Endothelial Growth Factor A; C408822:spiroiminodihydantoin; D006151:Guanosine; C046215:8-hydroxyguanosine; D014498:Uracil; D004247:DNA