title: Immunocytochemical localization of the calcium-binding proteins calbindin D28K, calretinin, and parvalbumin in bat visual cortex.
abstract: It is a common misconception that bats are blind, and various studies have suggested that bats have visual abilities. The purpose of this study was to investigate the cytoarchitecture of calbindin D28K (CB)-, calretinin (CR)-, and parvalbumin (PV)-immunoreactive (IR) neurons in the bat visual cortex using immunocytochemistry. The highest density of CB- and PV-IR neurons was located in layer IV of the visual cortex. The majority of CB- and PV-IR neurons were characterized by a stellate or round/oval shape. CR-IR neurons were predominantly located in layers II/III, and the cells were principally round/oval in shape. Two-color immunofluorescence revealed that 65.96%, 24.24%, and 77.00% of the CB-, CR-, and PV-IR neurons, respectively, contained gamma-aminobutyric acid (GABA). We observed calcium-binding protein (CBP)-IR neurons in specific layers of the bat visual cortex and in specific cell types. Many of the CBP-IR neurons were GABAergic interneurons. These data provide useful clues to aid in understanding the functional aspects of the bat visual system.
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea.
authors.forename: Hang-Gu
authors.initials: HG
authors.lastname: Kim
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Gu
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea.
authors.forename: Kyoung-Pil
authors.initials: KP
authors.lastname: Lee
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea.
authors.forename: Ji-Gun
authors.initials: JG
authors.lastname: Lee
authors.affiliation: Korea Science Academy of KAIST, Baegyanggwanmun-ro, Busanjin-gu, Busan, South Korea.
authors.forename: Chan-Wook
authors.initials: CW
authors.lastname: Kim
authors.affiliation: Korea Science Academy of KAIST, Baegyanggwanmun-ro, Busanjin-gu, Busan, South Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Korea Science Academy of KAIST, Baegyanggwanmun-ro, Busanjin-gu, Busan, South Korea.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Jeong
authors.affiliation: Korea Science Academy of KAIST, Baegyanggwanmun-ro, Busanjin-gu, Busan, South Korea.
authors.forename: Young-Wun
authors.initials: YW
authors.lastname: Jeong
authors.affiliation: Department of Biology, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, and Brain Science and Engineering Institute, Kyungpook National University, Daegu, South Korea. cjjeon@knu.ac.kr.
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Jeon
mesh_terms: D000818:Animals; D064092:Calbindin 1 / Q000032:analysis / Q000096:biosynthesis; D064032:Calbindin 2 / Q000032:analysis / Q000096:biosynthesis; D002685:Chiroptera; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010320:Parvalbumins / Q000032:analysis / Q000096:biosynthesis; D014793:Visual Cortex / Q000166:cytology* / Q000378:metabolism
chemical_list: D064092:Calbindin 1; D064032:Calbindin 2; D010320:Parvalbumins