title: Layer-by-layer inorganic/polymeric nanoparticles for kinetically controlled multigene delivery.
abstract: Nonviral gene delivery methods represent a potential safe and effective approach for treating myriad diseases. For many gene therapy applications, delivering multiple exogenous genes and controlling the time profile that these genes are expressed would be advantageous. Polymeric nonviral gene carriers are versatile and can be readily tailored for particular therapeutic applications, have the ability to carry multiple large genes within each particle, and can be more easily manufactured than viruses used for gene delivery. A layer-by-layer (LbL) theranostic-enabling nanoparticle was developed to incorporate two plasmid types which have differing expression time profiles. Temporally controlling the expression of exogenous DNA enables superior control over the microenvironment and could lead to better control over differentiation pathways and cell fate. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 707-713, 2016.
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.
authors.forename: Corey J
authors.initials: CJ
authors.lastname: Bishop
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.
authors.forename: Allen L
authors.initials: AL
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.
authors.forename: David S
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Murdock
authors.affiliation: Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Translational Tissue Engineering Center, Baltimore, Maryland, 21231.|Departments of Ophthalmology, Oncology, and Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, 21231.|Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, 21231.
authors.forename: Jordan J
authors.initials: JJ
authors.lastname: Green
mesh_terms: D045744:Cell Line, Tumor; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry; D006801:Humans; D057446:Hydrodynamics; D007287:Inorganic Chemicals / Q000737:chemistry*; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000378:metabolism; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection
chemical_list: D007287:Inorganic Chemicals; D009696:Nucleic Acids; D011108:Polymers; D006046:Gold
keywords: cell engineering; gene delivery; gold nanoparticle; layer-by-layer; poly(beta-amino ester)