title: Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides.
abstract: GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the r cor values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the r cor values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China ; West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine, West Anhui University, Lu'an 237012, China ; School of Pharmacy, Anhui Medical University, Hefei 230032, China.
authors.forename: Nai-Dong
authors.initials: ND
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
authors.forename: Nai-Fu
authors.initials: NF
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Anhui Medical University, Hefei 230032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Cao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, China.
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Huang