title: Expression and function of PDGF-α in columnar epithelial cells of age-related cataracts patients.
abstract: We studied the expression and function of platelet-derived growth factor A (PDGF-α) in the lens epithelial cells of cataracts patients. Ninety age-related cataracts patients were recruited in our hospital between January 2012 and January 2014. The expression levels of platelet-derived growth factor receptor (PDGFR) in the anterior capsule of the lens at different degrees of turbidity, and PDGF-α in the aqueous humor were detected. A human lens epithelium cell line was also cultured and studied. To investigate its function, PDGF-α was used to treat a PDGFR-silenced human lens epithelium cell line to observe changes in the proliferation, transfer, and epithelial mesenchymal transition (EMT). The expression of PDGF-α and its receptor increased in patients with more serious cataracts. Lens epithelium cells stimulated by PDGF-α showed greater proliferation and migration. The degree of EMT was also upregulated in cells stimulated by PDGF-α. However, silencing the expression of PDGFR inhibited the effects. The development and severity of age-related cataracts was related to the secretion and expression of PDGF-α. This may be a new therapeutic target for cataracts treatment.
authors.affiliation: Department of Ophthalmology, The Second Hospital of Suzhou University, Jiangsu, China.
authors.forename: J
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Ophthalmology, The Second Hospital of Suzhou University, Jiangsu, China.
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Ophthalmology, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Xu
authors.affiliation: Department of Ophthalmology, Shanghai Tenth People's Hospital Affiliated Tongji University, Shanghai, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Ophthalmology, The Second Hospital of Suzhou University, Jiangsu, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Xie
authors.affiliation: Department of Ophthalmology, The Second Hospital of Suzhou University, Jiangsu, China.
authors.forename: G X
authors.initials: GX
authors.lastname: Xu
mesh_terms: D000368:Aged; D000375:Aging; D015415:Biomarkers; D002386:Cataract / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D058750:Epithelial-Mesenchymal Transition; D019573:Epithelium, Corneal / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A