title: Volumetric structured illumination microscopy enabled by a tunable-focus lens.
abstract: We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.
authors.forename: Taylor
authors.initials: T
authors.lastname: Hinsdale
authors.forename: Bilal H
authors.initials: BH
authors.lastname: Malik
authors.forename: Cory
authors.initials: C
authors.lastname: Olsovsky
authors.forename: Javier A
authors.initials: JA
authors.lastname: Jo
authors.forename: Kristen C
authors.initials: KC
authors.lastname: Maitland
mesh_terms: D000818:Animals; D002417:Cattle; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D066298:In Vitro Techniques; D007909:Lenses; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D009061:Mouth Mucosa / Q000166:cytology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity