title: Autoantibodies Against Multiple Epitopes in Bp180 and Laminin Gamma-1 in Subepidermal Blistering Skin Disease Associated with Psoriatic Erythroderma.
abstract: We report a 79-year-old Japanese man who developed subepidermal blistering skin disease after an 8-year history of psoriasis. Histology of a bullous lesion revealed a subepidermal blister with a mixed inflammatory cell infiltrate and fibrin nets. Indirect immunofluorescence using normal human skin sections revealed IgG and IgA autoantibodies in the patient serum, which bound to the epidermal side of 1M NaCl-split skin sections. Immunoblot analysis revealed that both IgA and IgG antibodies reacted with the BP180 NC16a domain and the 120-kDa LAD-1 and that IgG antibodies also reacted with the BP180 C-terminal domain and laminin gamma-1. These findings indicated that autoantibodies to laminin gamma-1 and multiple epitopes in BP180 ectodomain played a role in the pathogenesis of this unique autoimmune subepidermal blistering skin disease associated with psoriasis.
authors.affiliation: Department of Dermatology, Kawaguchi Municipal Medical Center, 180 Nishiaraijyuku, Kawaguchi, Saitama 333-0833, Japan ; Division of Cutaneous Science, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo 173-0032, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Goto-Hamano
authors.affiliation: Department of Dermatology, Kawaguchi Municipal Medical Center, 180 Nishiaraijyuku, Kawaguchi, Saitama 333-0833, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Ito
authors.affiliation: Division of Cutaneous Science, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo 173-0032, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sakamoto-Kimura
authors.affiliation: Division of Cutaneous Science, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo 173-0032, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Terui
authors.affiliation: Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Bungo
authors.initials: B
authors.lastname: Ohyama
authors.affiliation: Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka 830-0011, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hashimoto
authors.affiliation: Functional Morphology, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo 173-0032, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hara
keywords: Multiple target antigens; psoriasis; subepidermal blistering skin disease