title: Somatic stem cell aging and malignant transformation--impact on therapeutic application.
abstract: Somatic stem cells possess unique properties of self-renewal and plasticity which make them promising candidates for use in tissue engineering and regenerative medicine, in addition to serving as efficient delivery vehicles in site-specific therapy. In the case of therapeutic application, it is essential to isolate and culture stem cells in vitro, to obtain them in sufficient quantities. Although long-term cultivation provides an adequate number of cells, it has been shown that this approach is associated with increased risk of transformation of cultured cells, which presents a significant biological hazard. This article reviews information about biological features and cellular events which occur during long-term cultivation of somatic stem cells, with respect to their safe utilization in potential clinical practice.
authors.forename: Marcela
authors.initials: M
authors.lastname: Kuniakova
authors.forename: Lenka
authors.initials: L
authors.lastname: Oravcova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Varchulova-Novakova
authors.forename: Diana
authors.initials: D
authors.lastname: Viglaska
authors.forename: Lubos
authors.initials: L
authors.lastname: Danisovic
mesh_terms: D002471:Cell Transformation, Neoplastic; D016922:Cellular Senescence; D006801:Humans; D034904:Lamin Type A / Q000378:metabolism; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D034904:Lamin Type A; D019098:Telomerase