title: [A negative correlation between Drosha expression and gastric adenocarcinoma malignancy and invasion].
abstract: OBJECTIVE
  To investigate the role of Drosha expression in the progression of gastric adenocarcinoma and its impact on the invasive ability of SGC-7901 human gastric cancer cells.
  
  
  METHODS
  Drosha expression was detected in 889 gastric carcinoma samples on tissue microarrays by immunohistochemistry staining and quantified by Image-Pro Plus software. Statistical analysis was used to evaluate the correlations between Drosha expression and the clinicopathological characteristics of the 889 tumor cases or the outcomes of 309 gastric adenocarcinoma patients. Drosha was knocked down in SGC-7901 cells by small interfering RNA (siRNA), and cell invasive ability was assessed by Transwell(TM) assay.
  
  
  RESULTS
  Drosha expression was the highest in the well differentiated gastric adenocarcinoma (median absorbance, 0.4195), and the lowest in the poorly differentiated samples. Drosha expression was significantly related to Laren classification, tumor size, tumor invasion depth, lymph node metastasis, tumor pathological grade and stage. Patients in Drosha positive group had a higher survival rate than those in Drosha negative group. Silencing Drosha in SGC-7901 cells resulted in an enhanced cell invasion.
  
  
  CONCLUSIONS
  Drosha expression was reduced gradually with the degrading histological differentiation of gastric adenocarcinoma, and the knock-down of Drosha expression could promote gastric adenocarcinoma cell invasion.
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Xu
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Zhou
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yan'e
authors.initials: Y
authors.lastname: DU
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Siyang
authors.initials: S
authors.lastname: Wen
authors.affiliation: College of Laboratory Medicine, Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Manran
authors.initials: M
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology*; D000328:Adult; D000368:Aged; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D043244:Ribonuclease III / Q000032:analysis / Q000502:physiology*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology*
chemical_list: C517918:DROSHA protein, human; D043244:Ribonuclease III