title: [MUC1-2VNTR DNA Vaccine Induces Immune Responses in Mouse Model with Multiple Myeloma].
abstract: OBJECTIVE
  To investigate the humoral and cellular immune responses induced by MUC1-2VNTR DNA vaccine in multiple myeloma (MM) tumor-bearing mice.
  
  
  METHODS
  In vitro, multiple myeloma cells were transfected by plasmid pcDNA3.1-2VNTR/myc-hisB with Lipofectamine2000. The above-mentioned mouse myeloma cells were inoculated subcutaneously into female BALB/c mice for establishing tumor-bearing animal models. These female BALB/c mice were immunized with pcDNA-2VNTR/myc-hisB or pcDNA/myc-hisB. The cytotoxic T lymphocyte (CTL) activity was detected by the LDH method and the spleen lymphocyte proliferation activity was detected by CCK-8 method.
  
  
  RESULTS
  After immunization of BALB/c tumor-bearing mice with recombinant plasmid for 25 days, the tumor mass (0.5605 ± 0.2065 g) was significantly lighter than that in the empty plasmid control group (1.521 ± 0.6985 g) (P < 0.01) and the control group (1.5315 ± 0.5425 g) (P < 0.01). The difference of tumor mass was not statislically significant between empty plasmid control group (1.521 ± 0.6985 g) and the control group (1.5315 ± 0.5425 g) (P > 0.05). The CTL and NK cell activity was significantly higher in the group of intramuscular injection with recombinant plasmid than that in control group. The spleen lymphocyte proliferation was statistically significantly increased after being immunized with recombinant plasmid pcDNA3.1-2VNTR/myc-hisB, compared with empty vector (P < 0.01). The results showed that MUC1-2VNTR gene immunization could induce anti-tumor effect in MM tumor-bearing mice.
  
  
  CONCLUSIONS
  MUC1-2VNTR DNA immunization can elicit both humoral and cellular tumor specific immune response to multiple myeloma in MM tumor-bearing mice. It suggested that the MUC1-2VNTR DNA vaccine may be a potential treatment measure for patients with MM.
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
authors.forename: Yue-Bo
authors.initials: YB
authors.lastname: Liu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
authors.forename: Ze-Ping
authors.initials: ZP
authors.lastname: Zhou
authors.affiliation: Department of Blood Transfusion, Qilu Hospital of Shandong University, Jinan 250012, Shangdong Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Mu
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
authors.forename: Gui-Yun
authors.initials: GY
authors.lastname: Huang
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China. E-mail: youzhangmd@aliyun.com.
authors.forename: You
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use*; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D018598:Minisatellite Repeats; D055262:Mucin-2 / Q000235:genetics*; D009101:Multiple Myeloma / Q000276:immunology / Q000628:therapy*; D009368:Neoplasm Transplantation; D010957:Plasmids; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D019444:Vaccines, DNA / Q000627:therapeutic use*
chemical_list: D019496:Cancer Vaccines; C524625:MUC2 protein, human; D055262:Mucin-2; D019444:Vaccines, DNA