title: Molecular typing of the actin gene of Trichomonas vaginalis isolates by PCR-RFLP in Iran.
abstract: Trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral, parasitic sexually transmitted infection in the world. At present, little is known regarding the degree of strain variability of T. vaginalis. A classification method for T. vaginalis strains would be a useful tool in the study of the epidemiology, drug resistance, pathogenesis and transmission of T. vaginalis. Eight different types of actin genes have been identified by PCR-RFLP in T. vaginalis; the purpose of this study is to determine the genotypes of this parasite in Karaj city, Iran. Forty-five clinical T. vaginalis isolates from vaginal secretions and urine sediment were collected from Karaj city from 2012 through 2014. DNA was extracted and the actin gene was amplified by nested-PCR; all samples were positive. To determine the genetic differences, sequencing on seven samples was conducted. Then, all PCR products were digested with HindII, MseI, and RsaI restriction enzymes. Of 45 isolates, 23 samples (51.1%) were of actin genotype G, 11 samples (24.4%) of genotype E, six samples (13.3%) of genotype H, three samples (6.6%) of genotype I, and two samples (4.4%) were mixed genotypes of G and E. Genetic diversity of T. vaginalis isolates is notable. The actin genotype G may be the dominant genotype in Karaj city, Iran.
authors.affiliation: Parasitology & Entomology Dept., Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Momeni
authors.affiliation: Parasitology & Entomology Dept., Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran. Electronic address: sadraeij@modares.ac.ir.
authors.forename: Javid
authors.initials: J
authors.lastname: Sadraei
authors.affiliation: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Bahram
authors.initials: B
authors.lastname: Kazemi
authors.affiliation: Parasitology & Entomology Dept., Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Abdolhossein
authors.initials: A
authors.lastname: Dalimi
mesh_terms: D000199:Actins / Q000235:genetics*; D000465:Algorithms; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D058889:Molecular Typing; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D016415:Sequence Alignment; D014245:Trichomonas Infections / Q000453:epidemiology / Q000469:parasitology*; D014247:Trichomonas Vaginitis / Q000453:epidemiology / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000145:classification / Q000235:genetics*; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000469:parasitology
chemical_list: D000199:Actins; D016054:DNA, Protozoan
keywords: Actin gene; Iran; Karaj city; Molecular typing; PCR-RFLP; Trichomonas vaginalis