title: Whole blood glucose analysis based on smartphone camera module.
abstract: Complementary metal oxide semiconductor (CMOS) image sensors have received great attention for their high efficiency in biological applications. The present work describes a CMOS image sensor-based whole blood glucose monitoring system through a point-of-care (POC) approach. A simple poly-ethylene terephthalate (PET) chip was developed to carry out the enzyme kinetic reaction at various concentrations (110–586 mg∕dL) of mouse blood glucose. In this technique, assay reagent is immobilized onto amine functionalized silica (AFSiO2) nanoparticles as an electrostatic attraction in order to achieve glucose oxidation on the chip. The assay reagent immobilized AFSiO2 nanoparticles develop a semi-transparent reaction platform, which is technically a suitable chip to analyze by a camera module. The oxidized glucose then produces a green color according to the glucose concentration and is analyzed by the camera module as a photon detection technique; the photon number decreases when the glucose concentration increases. The combination of these components, the CMOS image sensor and enzyme immobilized PET film chip, constitute a compact, accurate, inexpensive, precise, digital, highly sensitive, specific, and optical glucose-sensing approach for POC diagnosis.
authors.affiliation: Gachon University, Department of Bionanotechnology, Sungnam-461-701, Republic of Korea.
authors.forename: Jasmine Pramila
authors.initials: JP
authors.lastname: Devadhasan
authors.affiliation: Gachon Medical Research Institute, Gil Medical Center, Incheon, 405-760, Republic of KoreacKorea Mouse Metabolic Phenotyping Center, Gachon University, Lee Gil Ya Cancer and Diabetes Institute, Gil Medical Center, Incheon 405-760, Republic of Korea.
authors.forename: Hyunhee
authors.initials: H
authors.lastname: Oh
authors.affiliation: Gachon Medical Research Institute, Gil Medical Center, Incheon, 405-760, Republic of KoreacKorea Mouse Metabolic Phenotyping Center, Gachon University, Lee Gil Ya Cancer and Diabetes Institute, Gil Medical Center, Incheon 405-760, Republic of Korea.
authors.forename: Cheol Soo
authors.initials: CS
authors.lastname: Choi
authors.affiliation: Gachon University, Department of Bionanotechnology, Sungnam-461-701, Republic of KoreabGachon Medical Research Institute, Gil Medical Center, Incheon, 405-760, Republic of Korea.
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D015190:Blood Glucose Self-Monitoring / Q000295:instrumentation* / Q000379:methods; D003124:Colorimetry / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D063731:Mobile Applications; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000068997:Smartphone
chemical_list: D001786:Blood Glucose