title: Recombinase polymerase amplification: Emergence as a critical molecular technology for rapid, low-resource diagnostics.
abstract: Isothermal molecular diagnostics are bridging the technology gap between traditional diagnostics and polymerase chain reaction-based methods. These new techniques enable timely and accurate testing, especially in settings where there is a lack of infrastructure to support polymerase chain reaction facilities. Despite this, there is a significant lack of uptake of these technologies in developing countries where they are highly needed. Among these novel isothermal technologies, recombinase polymerase amplification (RPA) holds particular potential for use in developing countries. This rapid nucleic acid amplification approach is fast, highly sensitive and specific, and amenable to countries with a high burden of infectious diseases. Implementation of RPA technology in developing countries is critically required to assess limitations and potentials of the diagnosis of infectious disease, and may help identify impediments that prevent adoption of new molecular technologies in low resource- and low skill settings. This review focuses on approaching diagnosis of infectious disease with RPA.
authors.affiliation: a 1 Genecology Research Centre, Inflammation and Healing Research Cluster, School of Science and Engineering, University of the Sunshine Coast , Queensland, Australia.|b 2 Keystone Laboratories International, Kuje, FCT , Abuja, Nigeria.
authors.forename: Ameh
authors.initials: A
authors.lastname: James
authors.affiliation: a 1 Genecology Research Centre, Inflammation and Healing Research Cluster, School of Science and Engineering, University of the Sunshine Coast , Queensland, Australia.|c 3 Division of Experimental Therapeutics, Department of Medicine, Columbia University , NY, USA.
authors.forename: Joanne
authors.initials: J
authors.lastname: Macdonald
mesh_terms: D003906:Developing Countries; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000191:economics / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods; D045522:Recombinases; D012680:Sensitivity and Specificity
chemical_list: D045522:Recombinases
keywords: developing countries; isothermal amplification; molecular diagnostics; rapid test; recombinase polymerase amplification; resource constrained setting