title: Identification of Putative Olfactory Genes from the Oriental Fruit Moth Grapholita molesta via an Antennal Transcriptome Analysis.
abstract: BACKGROUND
  The oriental fruit moth, Grapholita molesta, is an extremely important oligophagous pest species of stone and pome fruits throughout the world. As a host-switching species, adult moths, especially females, depend on olfactory cues to a large extent in locating host plants, finding mates, and selecting oviposition sites. The identification of olfactory genes can facilitate investigation on mechanisms for chemical communications.
  
  
  RESULTS
  We generated transcriptome of female antennae of G.molesta using the next-generation sequencing technique, and assembled transcripts from RNA-seq reads using Trinity, SOAPdenovo-trans and Abyss-trans assemblers. We identified 124 putative olfactory genes. Among the identified olfactory genes, 118 were novel to this species, including 28 transcripts encoding for odorant binding proteins, 17 chemosensory proteins, 48 odorant receptors, four gustatory receptors, 24 ionotropic receptors, two sensory neuron membrane proteins, and one odor degrading enzyme. The identified genes were further confirmed through semi-quantitative reverse transcription PCR for transcripts coding for 26 OBPs and 17 CSPs. OBP transcripts showed an obvious antenna bias, whereas CSP transcripts were detected in different tissues.
  
  
  CONCLUSIONS
  Antennal transcriptome data derived from the oriental fruit moth constituted an abundant molecular resource for the identification of genes potentially involved in the olfaction process of the species. This study provides a foundation for future research on the molecules involved in olfactory recognition of this insect pest, and in particular, the feasibility of using semiochemicals to control this pest.
authors.affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.|Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.|Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.|Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, Shaanxi, People's Republic of China.|Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism*; D005260:Female; D005638:Fruit; D020869:Gene Expression Profiling / Q000379:methods; D019476:Insect Proteins / Q000235:genetics; D008297:Male; D009036:Moths / Q000235:genetics*; D009812:Odorants; D018035:Receptors, Odorant / Q000235:genetics; D012903:Smell / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D018035:Receptors, Odorant; C045648:odorant-binding protein