title: Enhanced Fibroblast Cellular Ligamentization Process to Polyethylene Terepthalate Artificial Ligament by Silk Fibroin Coating.
abstract: Artificial ligaments utilized in reconstruction of anterior cruciate ligament (ACL) are usually made of polyethylene terepthalate (PET) because of its good mechanical properties in vivo. However, it was found that the deficiencies in hydrophilicity and biocompatibility of PET hindered the process of ligamentization. Therefore, surface modification of the PET is deemed as a solution in resolving such problem. Silk fibroin (SF), which is characterized by good biocompatibility and low immunogenicity in clinical applications, was utilized to prepare a coating on the PET ligament (PET+SF) in this work. At first, decrease of hydrophobicity and appearance of amino groups were found on the surface of artificial PET ligament after coating with SF. Second, mouse fibroblasts were cultured on the two different kinds of ligament in order to clarify the possible effect of SF coating. It was proved that mouse fibroblasts display better adhesion and proliferation on PET+SF than PET ligament according to the results of several technical methods including SEM observation, cell adhesive force and spread area test, and mRNA analysis. Meanwhile, methylthiazolyldiphenyl-tetrazolium bromide and DNA content tests showed that biocompatibility of PET+SF is better than PET ligament. In addition, collagen deposition tests also indicated that the quantity of collagen in PET+SF is higher than PET ligament. Based on these results, it can be concluded that SF coating is suggested to be an effective approach to modify the surface of PET ligament and enhance the "ligamentization" process in vivo accordingly.
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.|State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Chengchong
authors.identifier: http://orcid.org/0000-0003-2143-103X
authors.initials: C
authors.lastname: Ai
authors.affiliation: Taihe Hospital, Affiliated Hospital of Hubei University of Medicine, Shiyan, Hubei Province, China.
authors.forename: Zufeng
authors.initials: Z
authors.lastname: Zhan
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wan
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai, China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Advanced Materials Laboratory, Department of Macromolecular Science, Fudan University, Shanghai, China.
authors.forename: Zhengzhong
authors.initials: Z
authors.lastname: Shao
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Tianwu
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Forest Products, Anhui Agricultural University, Hefei, Anhui Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shiyan, Hubei Province, China.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology*; D005349:Fibroins / Q000494:pharmacology*; D008022:Ligaments; D008422:Materials Testing; D051379:Mice; D011093:Polyethylene Terephthalates / Q000494:pharmacology*
chemical_list: D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D005349:Fibroins
keywords: Fibroblast cell; Ligamentization; Polyethylene terepthalate artificial ligament; Silk fibroin