title: Association between pre-miR-27a functional polymorphism and risk of colorectal cancer in north Chinese Han population.
abstract: MicroRNA-27a (miR-27a) is deemed as an oncogene in malignancies including colorectal cancer (CRC), and rs895819 within pre-miR-27a may affect its secondary structure, leading to its aberrant expression and dysfunction of its targeted gene. We investigated genotype and allele frequencies of the locus in 412 I-III stage CRC cases and 412 age- and sex-matched healthy individuals to explore the possible association between them in the north of Chinese population. The results showed that frequencies of alleles A and G and genotypes GG, AG, and AA of the locus were 65.7%, 34.3%, 17.0%, 34.7%, and 48.3% in cases and 69.9%, 30.1%, 9.9%, 40.2%, and 49.8% in controls, respectively. GG genotype of the locus was positively associated with an increased risk of CRC in codominant (P=0.01, adjusted odds ratio =1.541, 95% confidence interval =1.110-2.239 for genotype GG vs AA) and recessive (P=0.003, adjusted odds ratio =1.855, 95% confidence interval =1.221-2.786 for genotype GG vs AA/GA) models, indicating that GG genotype of the locus might increase susceptibility to CRC. Moreover, genotypes AG and GG and allele G were significantly associated with III stage (P<0.001, P<0.001, and P=0.001, respectively), suggesting that the locus was associated with the progression of CRC. These results suggested that rs895819 within pre-miR-27a was involved in colorectal carcinogenesis and progression, genotype GG of the locus might be a susceptible factor for CRC, and allele G and allele G carrier (genotypes AG and GG) could predict CRC progression in north Chinese Han population.
authors.affiliation: Department of General Surgery, Tianjin Hospital, Tianjin, People's Republic of China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Bian
authors.affiliation: Department of General Surgery, Beijing Chaoyang Hospital, Beijing, People's Republic of China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Tianjin Hospital, Tianjin, People's Republic of China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Gu
authors.affiliation: Department of Gastrointestinal Surgery, Union Medical Center of Tianjin, Nankai University Affiliated Hospital, Tianjin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
keywords: colorectal cancer; miR-27a; polymorphism