title: Coagulase-Positive Staphylococcus: Prevalence and Antimicrobial Resistance.
abstract: Staphylococcus pseudintermedius is the most prevalent coagulase-positive Staphylococcus inhabitant of the skin and mucosa of dogs and cats, causing skin and soft tissue infections in these animals. In this study, coagulase-positive Staphylococcus species were isolated from companion animals, veterinary professionals, and objects from a clinical veterinary environment by using two particular culture media, Baird-Parker RPF agar and CHROMagar Staph aureus. Different morphology features of colonies on the media allowed the identification of the species, which was confirmed by performing a multiplex polymerase chain reaction (PCR). Among 23 animals, 15 (65.2%) harbored coagulase-positive Staphylococcus, being 12 Staphylococcus pseudintermedius carriers. Four out of 12 were methicillin-resistant S. pseudintermedius (MRSP). All veterinary professionals had coagulase-positive Staphylococcus (CoPS) species on their hands and two out of nine objects sampled harbored MRSP. The antimicrobial-resistance pattern was achieved for all isolates, revealing the presence of many multidrug-resistant CoPS, particularly S. pseudintermedius . The combined analysis of the antimicrobial-resistance patterns shown by the isolates led to the hypothesis that there is a possible crosscontamination and dissemination of S. aureus and S. pseudintermedius species between the three types of carriers sampled in this study that could facilitate the spread of the methicillin-resistance phenotype.
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Nuno
authors.initials: N
authors.lastname: Beça
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Lucinda Janete
authors.initials: LJ
authors.lastname: Bessa
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Ângelo
authors.initials: Â
authors.lastname: Mendes
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Santos
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Leite-Martins
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Augusto J F
authors.initials: AJ
authors.lastname: Matos
authors.affiliation: From the ICBAS-Abel Salazar Institute for the Biomedical Sciences (P.M.C., N.B., A.M., J.S., L.L-M., A.J.F.M.); and CIIMAR-Interdisciplinary Center for Marine and Environmental Research (L.J.B), University of Porto, Portugal.
authors.forename: Paulo Martins
authors.initials: PM
authors.lastname: da Costa
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003030:Coagulase / Q000378:metabolism; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D006762:Hospitals, Animal; D006801:Humans; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification* / Q000187:drug effects / Q000201:enzymology
chemical_list: D000900:Anti-Bacterial Agents; D003030:Coagulase