title: Proteomic Analysis of the Relationship between Metabolism and Nonhost Resistance in Soybean Exposed to Bipolaris maydis.
abstract: Nonhost resistance (NHR) pertains to the most common form of plant resistance against pathogenic microorganisms of other species. Bipolaris maydis is a non-adapted pathogen affecting soybeans, particularly of maize/soybean intercropping systems. However, no experimental evidence has described the immune response of soybeans against B. maydis. To elucidate the molecular mechanism underlying NHR in soybeans, proteomics analysis based on two-dimensional polyacrylamide gel electrophoresis (2-DE) was performed to identify proteins involved in the soybean response to B. maydis. The spread of B. maydis spores across soybean leaves induced NHR throughout the plant, which mobilized almost all organelles and various metabolic processes in response to B. maydis. Some enzymes, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), mitochondrial processing peptidase (MPP), oxygen evolving enhancer (OEE), and nucleoside diphosphate kinase (NDKs), were found to be related to NHR in soybeans. These enzymes have been identified in previous studies, and STRING analysis showed that most of the protein functions related to major metabolic processes were induced as a response to B. maydis, which suggested an array of complex interactions between soybeans and B. maydis. These findings suggest a systematic NHR against non-adapted pathogens in soybeans. This response was characterized by an overlap between metabolic processes and response to stimulus. Several metabolic processes provide the soybean with innate immunity to the non-adapted pathogen, B. maydis. This research investigation on NHR in soybeans may foster a better understanding of plant innate immunity, as well as the interactions between plant and non-adapted pathogens in intercropping systems.
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Dong
authors.affiliation: The Life Science and Technology Department of Kunming University, Kunming, 650214, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Mei
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Xiahong
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Manhua
authors.initials: M
authors.lastname: Pan
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Youyong
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
authors.forename: Chengyun
authors.initials: C
authors.lastname: Li
mesh_terms: D001203:Ascomycota / Q000276:immunology; D060467:Disease Resistance; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013025:Glycine max / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins; D020543:Proteome