title: A polydopamine nanosphere based highly sensitive and selective aptamer cytosensor with enzyme amplification.
abstract: With CCRF-CEM as the model cell, a highly sensitive and selective cytosensor was developed by taking advantage of polydopamine nanospheres for the first time. The strategies of aptamer/membrane protein recognition and Exonuclease III assisted cycle amplification were used for improving selectivity and sensitivity. The detection of limit reached was as low as 15 cells per mL.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China. ekwang@ciac.ac.cn.
authors.forename: Daoqing
authors.initials: D
authors.lastname: Fan
authors.forename: Changtong
authors.initials: C
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Gu
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Liu
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D002417:Cattle; D015815:Cell Adhesion Molecules / Q000737:chemistry; D045744:Cell Line, Tumor; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D057230:Limit of Detection; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D015815:Cell Adhesion Molecules; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D012710:Serum Albumin, Bovine; C096905:PTK7 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III