title: The Structure, Pathogenicity and Immunogenicity of Two Virion Fractions Harvested from Cell Cultures Infected with the CA16 Virus.
abstract: OBJECTIVE
  To investigate the biological characteristics of the two types of virion fractions of Coxsackievirus A 16 (CA16), which include the real virion fraction and pseudo-virion fraction in their structure, pathogenicity and immunogenicity.
  
  
  METHODS
  We obtained the two CA16 virion fractions by density gradient centrifugation. The morphology of virion fractions was analyzed by electron microscopy, while the antigenic characteristics and immunogenicity of two virion fractions were determined by ELISA, SDS-PAGE, Western blot, qRT-PCR, and the mouse model of immune response.
  
  
  RESULTS
  The two virion fractions contained the major viral antigen components in their structures, showed similar pathogenicity in a neonatal murine model and were capable of inducing an effective primary immune response in adult mice, regardless of the essential distinction between the two virion fractions, which was the cleavage of VP0 to VP2 and VP4.
  
  
  CONCLUSIONS
  The two CA16 virion fractions showed antigenicity and immunogenicity with inducing a specific immune response in animals.
authors.affiliation: Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Pu
authors.forename: Hongtai
authors.initials: H
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.forename: Erxia
authors.initials: E
authors.lastname: Yang
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Che
authors.forename: Chenghong
authors.initials: C
authors.lastname: Dong
authors.forename: Yun
authors.initials: Y
authors.lastname: Liao
authors.forename: Longding
authors.initials: L
authors.lastname: Liu
authors.forename: Lichun
authors.initials: L
authors.lastname: Wang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D001921:Brain / Q000821:virology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003384:Coxsackievirus Infections / Q000276:immunology* / Q000821:virology*; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D029821:Enterovirus A, Human / Q000276:immunology; D008168:Lung / Q000648:ultrastructure / Q000821:virology; D051379:Mice; D014771:Virion / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral