title: Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b.
abstract: Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Su
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Tuizi
authors.initials: T
authors.lastname: Feng
authors.affiliation: Department of Entomology, University of Kentucky, Lexington, KY, 40546, USA.
authors.forename: Xuguo
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Ju'e
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Lu
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Integrated Management of Pest and Disease on Horticultural Crops in Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000821:virology; D012241:Rhodopseudomonas / Q000378:metabolism*; D016415:Sequence Alignment; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000254:growth & development / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000998:Antiviral Agents; D001426:Bacterial Proteins