title: Functional characterization of a new promoter isolated from torque teno sus virus 1.
abstract: Torque teno sus virus 1 (TTSuV1) has a non-enveloped, single-stranded, negative-sense circular DNA genome, and it is widely distributed in pigs. Open reading frame 1 (ORF1) of TTSuV1 can be transcribed into mRNA and then translated into protein; however, its promoter has not yet been identified. We used a dual-luciferase reporter system, involving pGL3-Basic and pRL-TK, to identify the promoter of TTSuV1 ORF1. Our results revealed that the sequence between nucleotides 196 and 525 promoted the transcription of the firefly luciferase gene. The core sequence of the promoter was between nucleotides 250 and 400. A comparison of the identified TTSuV1 ORF1 promoter with that from cytomegalovirus (CMV) suggested that the two promoters were similar in strength. Our findings provide new information regarding the molecular biology of TTSuV1 and have revealed a new promoter that can be used in plasmids for numerous applications.
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Yanwu
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Chen
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Wu
authors.affiliation: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, 150001, China. lcm@hvri.ac.cn.
authors.forename: Changming
authors.initials: C
authors.lastname: Liu
mesh_terms: D019677:Artificial Gene Fusion; D017930:Genes, Reporter; D049409:Luciferases, Firefly / Q000032:analysis / Q000235:genetics; D011401:Promoter Regions, Genetic; D022783:Torque teno virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: C446939:ORF1 protein, TT virus; D014764:Viral Proteins; D049409:Luciferases, Firefly