title: Protective Effect of Artemisia asiatica Extract and Its Active Compound Eupatilin against Cisplatin-Induced Renal Damage.
abstract: The present study investigated the renoprotective effect of an Artemisia asiatica extract and eupatilin in kidney epithelial (LLC-PK1) cells. Although cisplatin is effective against several cancers, its use is limited due to severe nephrotoxicity. Eupatilin is a flavonoid compound isolated from the Artemisia plant and possesses antioxidant as well as potent anticancer properties. In the LLC-PK1 cellular model, the decline in cell viability induced by oxidative stress, such as that induced by cisplatin, was significantly and dose-dependently inhibited by the A. asiatica extract and eupatilin. The increased protein expressions of phosphorylated JNK and p38 by cisplatin in cells were markedly reduced after A. asiatica extract or eupatilin cotreatment. The elevated expression of cleaved caspase-3 was significantly reduced by A. asiatica extract and eupatilin, and the elevated percentage of apoptotic cells after cisplatin treatment in LLC-PK1 cells was markedly decreased by cotreatment with A. asiatica extract or eupatilin. Taken together, these results suggest that A. asiatica extract and eupatilin could cure or prevent cisplatin-induced renal toxicity without any adverse effect; thus, it can be used in combination with cisplatin to prevent nephrotoxicity.
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Jun Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Dahae
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Surgery, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 210-711, Republic of Korea.
authors.forename: Hyuk-Jai
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Department of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea.
authors.forename: Hak Cheol
authors.initials: HC
authors.lastname: Kwon
authors.affiliation: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung 210-340, Republic of Korea.
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Gwi Seo
authors.initials: GS
authors.lastname: Hwang
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Ki Sung
authors.initials: KS
authors.lastname: Kang
authors.affiliation: Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 210-711, Republic of Korea.
authors.forename: Dae-Woon
authors.initials: DW
authors.lastname: Eom