title: FcRγ-chain deficiency reduces the development of diet-induced obesity.
abstract: OBJECTIVE
  Pathogenic immunoglobulins are produced during the development of obesity and contribute to the development of insulin resistance (IR). However, the mechanisms by which these antibodies affect IR are largely unknown. This study investigated whether Fc-receptors contribute to the development of diet-induced obesity and IR by studying FcRγ(-/-) mice that lack the γ-subunit necessary for signaling and cell surface expression of FcγR and FcεRI.
  
  
  METHODS
  FcRγ(-/-) and wild-type (WT) mice were fed a high-fat diet (HFD) to induce obesity. At 4 and 11 weeks, body weight and insulin sensitivity were measured, and adipose tissue (AT) inflammation was determined. Furthermore, intestinal triglyceride (TG) uptake and plasma TG clearance were determined, and gut microbiota composition was analyzed.
  
  
  RESULTS
  FcRγ(-/-) mice gained less weight after 11 weeks of HFD. They had reduced adiposity, adipose tissue inflammation, and IR. Interestingly, FcRγ(-/-) mice had higher lean mass compared to WT mice, which was associated with increased energy expenditure. Intestinal TG absorption was increased whereas plasma TG clearance was not affected in FcRγ(-/-) mice. Gut microbial composition differed significantly and might therefore have added to the observed phenotype.
  
  
  CONCLUSIONS
  FcRγ-chain deficiency reduces the development of diet-induced obesity, as well as associated AT inflammation and IR at 11 weeks of HFD.
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.|Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Lianne
authors.initials: L
authors.lastname: van Beek
authors.affiliation: Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.|Department of Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Irene O C M
authors.initials: IO
authors.lastname: Vroegrijk
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.|Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Saeed
authors.initials: S
authors.lastname: Katiraei
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.|Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Mattijs M
authors.initials: MM
authors.lastname: Heemskerk
authors.affiliation: Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.|Department of Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Andrea D
authors.initials: AD
authors.lastname: van Dam
authors.affiliation: Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.|Department of Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Sander
authors.initials: S
authors.lastname: Kooijman
authors.affiliation: Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.|Department of Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Patrick C N
authors.initials: PC
authors.lastname: Rensen
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Frits
authors.initials: F
authors.lastname: Koning
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: J Sjef
authors.initials: JS
authors.lastname: Verbeek
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.|Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.|Department of Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Ko
authors.initials: K
authors.lastname: Willems van Dijk
authors.affiliation: Department of Human Genetics, Leiden University Medical Center, Leiden, the Netherlands.|Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Vanessa
authors.initials: V
authors.lastname: van Harmelen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D050154:Adiposity / Q000235:genetics; D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects*; D007333:Insulin Resistance / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009765:Obesity / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D015434:Panniculitis / Q000235:genetics; D017455:Receptors, IgE / Q000378:metabolism; D017452:Receptors, IgG / Q000172:deficiency*; D015398:Signal Transduction / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism
chemical_list: C521907:Fc-epsilon receptor I beta-chain, mouse; D017455:Receptors, IgE; D017452:Receptors, IgG; D014280:Triglycerides