title: Peptide/protein vaccine delivery system based on PLGA particles.
abstract: Due to the excellent safety profile of poly (D,L-lactide-co-glycolide) (PLGA) particles in human, and their biodegradability, many studies have focused on the application of PLGA particles as a controlled-release vaccine delivery system. Antigenic proteins/peptides can be encapsulated into or adsorbed to the surface of PLGA particles. The gradual release of loaded antigens from PLGA particles is necessary for the induction of efficient immunity. Various factors can influence protein release rates from PLGA particles, which can be defined intrinsic features of the polymer, particle characteristics as well as protein and environmental related factors. The use of PLGA particles encapsulating antigens of different diseases such as hepatitis B, tuberculosis, chlamydia, malaria, leishmania, toxoplasma and allergy antigens will be described herein. The co-delivery of antigens and immunostimulants (IS) with PLGA particles can prevent the systemic adverse effects of immunopotentiators and activate both dendritic cells (DCs) and natural killer (NKs) cells, consequently enhancing the therapeutic efficacy of antigen-loaded PLGA particles. We will review co-delivery of different TLR ligands with antigens in various models, highlighting the specific strengths and weaknesses of the system. Strategies to enhance the immunotherapeutic effect of DC-based vaccine using PLGA particles can be designed to target DCs by functionalized PLGA particle encapsulating siRNAs of suppressive gene, and disease specific antigens. Finally, specific examples of cellular targeting where decorating the surface of PLGA particles target orally administrated vaccine to M-cells will be highlighted.
authors.affiliation: a Department of Recombinant Protein Production , Research & Production Complex, Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Allahyari
authors.affiliation: b Department of Pharmaceutical Biotechnology , School of Pharmacy, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Mohit
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage*; D004337:Drug Carriers / Q000008:administration & dosage*; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage*; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011506:Proteins / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D011506:Proteins; D022223:Vaccines, Subunit; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: PLGA particle; TLR ligands; delivery system; micro/nano particles; protein/peptide vaccine