title: Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize.
abstract: Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20-30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program. In this study, a diverse maize population comprising of 527 inbred lines was evaluated in four environments and a genome-wide association study (GWAS) was undertaken with over 556000 SNP markers. Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions. The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results. Significant correlation (R2 = 0.79) between the MRDD severity and the number of resistance alleles was observed. Consequently, we have broadened the resistant germplasm to MRDD and identified a number of resistance alleles by GWAS. The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Gengshen
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Hao
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China; College of Agronomy, Synergetic Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Ding
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D002681:China; D060467:Disease Resistance / Q000235:genetics*; D055106:Genome-Wide Association Study; D010935:Plant Diseases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D003313:Zea mays / Q000235:genetics*