title: Imaging of Electrotransferred siRNA.
abstract: SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer. SiRNA is electrophoretically drifted directly to the cytoplasm during the pulse. No post pulse transfer is observed. The uploaded siRNA then freely diffuse in the cytoplasm with no access to the nuclei.
authors.affiliation: IPBS (Institut de Pharmacologie et de Biologie Structurale), CNRS, BP 64182, 205 route de Narbonne, 31077, Toulouse, France.|IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, UPS (Université Paul Sabatier), BP 64182, 31077, Toulouse, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Golzio
authors.affiliation: IPBS (Institut de Pharmacologie et de Biologie Structurale), CNRS, BP 64182, 205 route de Narbonne, 31077, Toulouse, France. justin.teissie@ipbs.fr.|IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, UPS (Université Paul Sabatier), BP 64182, 31077, Toulouse, France. justin.teissie@ipbs.fr.
authors.forename: Justin
authors.initials: J
authors.lastname: Teissié
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018274:Electroporation / Q000379:methods; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008546:Melanoma, Experimental; D051379:Mice; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering
keywords: Confocal fluorescence imaging; Electroporation; Electropulsation; siRNA