title: Micromorphology of the elytral cuticle of beetles, with an emphasis on weevils (Coleoptera: Curculionoidea).
abstract: The elytral cuticle of 40 beetle species, comprising 14 weevils (Curculionoidea) and 26 representatives of other taxa, is examined. All weevils and 18 other species have an endocuticle with prominent macrofibers, which corresponds to a modified pseudo-orthogonal cuticle. Angles between successive layers of macrofibers range between 30° and 90°, but are constantly less than 60° in weevils. In all Curculionoidea, as well as in one buprestid and one erotylid species exo- and endocuticle are densely interlocked. In the weevil Sitophilus granarius, transmission electron microscopy revealed vertical microfibrils extending from the exocuticle between the macrofibers of the underlaying endocuticle. Vertical microfibrils connecting successive macrofiber layers of the endocuticle were observed in S. granarius and Trigonopterus nasutus. Distinct cuticular characters are traced on a beetle phylogeny: the angles between unidirectional endocuticle layers; the presence and the shape of endocuticular macrofibers; and the interlocking of exo- and endocuticle. While character traits seem to be more or less randomly distributed among Coleoptera, the Curculionoidea have a uniform groundplan: The "weevil-specific" combination of characters includes 1) interlocking of exo- and endocuticle, 2) an endocuticle with distinct ovoid macrofibers embedded in a matrix and 3) comparatively small angles between successive endocuticular layers. Thus, phylogenetic constraints appear equally important to functional factors in the construction of the weevil elytron.
authors.affiliation: ANKA/Institute for Photon Science and Synchrotron Radiation, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany; Laboratory for Applications of Synchrotron Radiation, Karlsruhe Institute of Technology, Kaiserstr. 12, D-76131 Karlsruhe, Germany; State Museum of Natural History (SMNK), Erbprinzenstr. 13, D-76133 Karlsruhe, Germany; Institute for Cell Biology, Heinrich Heine University, Universitätsstr. 1, D-40225 Düsseldorf, Germany. Electronic address: thomas.vandekamp@kit.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: van de Kamp
authors.affiliation: State Museum of Natural History (SMNK), Erbprinzenstr. 13, D-76133 Karlsruhe, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Riedel
authors.affiliation: Institute for Cell Biology, Heinrich Heine University, Universitätsstr. 1, D-40225 Düsseldorf, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Greven
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D048889:Weevils / Q000648:ultrastructure; D014921:Wings, Animal / Q000648:ultrastructure
keywords: Coleoptera; Curculionoidea; Cuticle; Elytra; Macrofibers