title: Translation elongation factor eEF1A1 is a novel partner of a multifunctional protein Sgt1.
abstract: Mammalian translation elongation factor eEF1A is involved in ribosomal polypeptide synthesis. Also, the protein fulfills many additional duties in an eukaryotic cell. Here, we identified a novel partner of the eEF1A1 isoform, namely Sgt1, a protein that possesses co-chaperon properties and participates in antiviral defense processes. By applying different methods, we demonstrated the interaction between eEF1A1 and Sgt1 using both purified proteins and cell lysates. We also found that the D2 and D3 domains of eEF1A1 and the TPR domain of Sgt1 are involved in complex formation. Modeling of the Sgt1-eEF1A1 complex suggested both shape and charge complementarities of the eEF1A1-Sgt1 interface stabilized by a number of salt bridges. As long as such interaction mode is typical more for protein-nucleic acid interaction we suggested a possibility that Sgt1 competes with viral RNA for binding to eEF1A and obtained in vitro evidence to this effect.
authors.affiliation: State Key Laboratory on Molecular and Cell Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., Kiev 03680, Ukraine.
authors.forename: Oleksandra
authors.initials: O
authors.lastname: Novosylna
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur str., 02-093 Warsaw, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Jurewicz
authors.affiliation: State Key Laboratory on Molecular and Cell Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., Kiev 03680, Ukraine; Laboratory of Bioinformatics and Structural Biology, Institute of Food Biotechnology and Genomics, 2A Оsipovskogo str., Kiev 04123, Ukraine.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Pydiura
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur str., 02-093 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Goral
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur str., 02-093 Warsaw, Poland. Electronic address: a.filipek@nencki.gov.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Filipek
authors.affiliation: State Key Laboratory on Molecular and Cell Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., Kiev 03680, Ukraine. Electronic address: negrutskii@imbg.org.ua.
authors.forename: Boris
authors.initials: B
authors.lastname: Negrutskii
authors.affiliation: State Key Laboratory on Molecular and Cell Biology, Institute of Molecular Biology and Genetics, 150 Zabolotnogo str., Kiev 03680, Ukraine.
authors.forename: Anna
authors.initials: A
authors.lastname: El'skaya
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D062105:Molecular Docking Simulation; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C094814:EEF1A1 protein, human; D008024:Ligands; D020648:Peptide Elongation Factor 1; D010446:Peptide Fragments; D020033:Protein Isoforms; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C490164:SUGT1 protein, human; D005982:Glutathione Transferase
keywords: Protein–protein interaction; Sgt1; eEF1A