title: Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae.
abstract: Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is a promising approach to engineering heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed a xylose-fermenting yeast SyBE001 through combinatorial fine-tuning the expression of XylA and endogenous XKS1. Additional overexpression of genes RKI1, RPE1, TKL1, and TAL1 in the non-oxidative pentose phosphate pathway (PPP) in SyBE001 increased the xylose consumption rate by 1.19-fold. By repetitive adaptation, the xylose utilization rate was further increased by ∼10-fold in the resultant strain SyBE003. Gene expression analysis identified a variety of genes with significantly changed expression in the PPP, glycolysis and the tricarboxylic acid cycle in SyBE003.
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zha
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Gao-Gang
authors.initials: GG
authors.lastname: Liu
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Bing-Zhi
authors.initials: BZ
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University Tianjin, China ; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University Tianjin, China.
authors.forename: Ying-Jin
authors.initials: YJ
authors.lastname: Yuan
keywords: XylA; evolutionary engineering; synthetic biology; xylose isomerase; xylose utilization; yeast