title: Modulation of triglyceride accumulation in adipocytes by psychopharmacological agents in vitro.
abstract: Weight gain is a major problem during psychopharmacological treatment. Research has concentrated on the appetite inducing properties and mechanisms of these drugs in the central nervous system. The potential contribution of direct effects of drugs on metabolically relevant peripheral cells such as adipocytes is less well understood. We examined the influence of the antidepressant imipramine, the antipsychotic clozapine, and the mood stabilizer lithium on preadipocytes and adipocytes in vitro, using Simpson-Golabi-Behmel syndrome (SGBS) cells, an established human preadipocyte model. Parameters of cell differentiation and signaling, and cell metabolism were measured. We found significantly increased triglyceride accumulation in adipocytes after supplementation with imipramine and lithium at therapeutic concentrations, compared to non-supplemented control samples. However, gene expression levels of an early marker of adipogenesis, the peroxisome proliferator-activated receptor gamma (PPAR-γ) and a late marker of adipogenesis, the fatty acid binding protein 4 (FABP4), as well as expression of adiponectin (ADIPOQ) did not change significantly in the presence of these psychopharmacological agents. The results suggest a direct influence of imipramine and lithium but not clozapine on fat storage of adipocytes. The underlying mechanisms of fatty acid storage and adipocyte differentiation however remain to be elucidated.
authors.affiliation: Integrated Research and Treatment Center (IFB) AdiposityDiseases, Leipzig, Germany; Center for Pediatric Research Leipzig, University Hospital for Children & Adolescents, Leipzig, Germany.
authors.forename: Dennis
authors.initials: D
authors.lastname: Löffler
authors.affiliation: Integrated Research and Treatment Center (IFB) AdiposityDiseases, Leipzig, Germany; Center for Pediatric Research Leipzig, University Hospital for Children & Adolescents, Leipzig, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Landgraf
authors.affiliation: Integrated Research and Treatment Center (IFB) AdiposityDiseases, Leipzig, Germany; Center for Pediatric Research Leipzig, University Hospital for Children & Adolescents, Leipzig, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Körner
authors.affiliation: Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kratzsch
authors.affiliation: Department of Psychiatry, University of Tasmania, Hobart, Tasmania, Australia.
authors.forename: Kenneth Clifford
authors.initials: KC
authors.lastname: Kirkby
authors.affiliation: Integrated Research and Treatment Center (IFB) AdiposityDiseases, Leipzig, Germany; Department of Psychiatry and Psychotherapy, University of Leipzig, Leipzig, Germany. Electronic address: Hubertus.Himmerich@medizin.uni-leipzig.de.
authors.forename: Hubertus
authors.initials: H
authors.lastname: Himmerich
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology; D052242:Adiponectin / Q000378:metabolism; D002460:Cell Line; D003024:Clozapine / Q000009:adverse effects* / Q000494:pharmacology; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007099:Imipramine / Q000009:adverse effects* / Q000494:pharmacology; D018020:Lithium Compounds / Q000009:adverse effects* / Q000494:pharmacology; D047495:PPAR gamma / Q000378:metabolism; D011619:Psychotropic Drugs / Q000009:adverse effects* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D018020:Lithium Compounds; D047495:PPAR gamma; D011619:Psychotropic Drugs; D012333:RNA, Messenger; D014280:Triglycerides; D003024:Clozapine; D007099:Imipramine
keywords: Adipocytes; Adipose tissue; Clozapine; Imipramine; Lithium; Weight gain