title: Reporter systems for in vivo tracking of lactic acid bacteria in animal model studies.
abstract: Bioluminescence (BLI) and fluorescence imaging (FI) allow for non-invasive detection of viable microorganisms from within living tissue and are thus ideally suited for in vivo probiotic studies. Highly sensitive optical imaging techniques detect signals from the excitation of fluorescent proteins, or luciferase-catalyzed oxidation reactions. The excellent relation between microbial numbers and photon emission allow for quantification of tagged bacteria in vivo with extreme accuracy. More information is gained over a shorter period compared to traditional pre-clinical animal studies. The review summarizes the latest advances in in vivo bioluminescence and fluorescence imaging and points out the advantages and limitations of different techniques. The practical application of BLI and FI in the tracking of lactic acid bacteria in animal models is addressed.
authors.affiliation: a Department of Microbiology ; Stellenbosch University ; Matieland, Stellenbosch , South Africa.
authors.forename: Winschau F
authors.initials: WF
authors.lastname: van Zyl
authors.affiliation: a Department of Microbiology ; Stellenbosch University ; Matieland, Stellenbosch , South Africa.
authors.forename: Shelly M
authors.initials: SM
authors.lastname: Deane
authors.affiliation: a Department of Microbiology ; Stellenbosch University ; Matieland, Stellenbosch , South Africa.
authors.forename: Leon M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000382:microbiology*; D017930:Genes, Reporter; D056546:Lactobacillales / Q000254:growth & development*; D008163:Luminescent Measurements / Q000379:methods*; D023421:Models, Animal; D061848:Optical Imaging / Q000379:methods*; D019936:Probiotics / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
keywords: lactic acid bacteria; reporter systems