title: Modulatory effect of iron chelators on adenosine deaminase activity and gene expression in Trichomonas vaginalis.
abstract: Trichomonas vaginalis is a flagellate protozoan that parasitises the urogenital human tract and causes trichomoniasis. During the infection, the acquisition of nutrients, such as iron and purine and pyrimidine nucleosides, is essential for the survival of the parasite. The enzymes for purinergic signalling, including adenosine deaminase (ADA), which degrades adenosine to inosine, have been characterised in T. vaginalis. In the evaluation of the ADA profile in different T. vaginalis isolates treated with different iron sources or with limited iron availability, a decrease in activity and an increase in ADA gene expression after iron limitation by 2,2-bipyridyl and ferrozine chelators were observed. This supported the hypothesis that iron can modulate the activity of the enzymes involved in purinergic signalling. Under bovine serum limitation conditions, no significant differences were observed. The results obtained in this study allow for the assessment of important aspects of ADA and contribute to a better understanding of the purinergic system in T. vaginalis and the role of iron in establishing infection and parasite survival.
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Muriel
authors.initials: M
authors.lastname: Primon-Barros
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Graziela Vargas
authors.initials: GV
authors.lastname: Rigo
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Amanda Piccoli
authors.initials: AP
authors.lastname: Frasson
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Odelta dos
authors.initials: Od
authors.lastname: Santos
authors.affiliation: Laboratório de Biologia Molecular, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brasil.
authors.forename: Lisiane
authors.initials: L
authors.lastname: Smiderle
authors.affiliation: Laboratório de Biologia Molecular, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brasil.
authors.forename: Silvana
authors.initials: S
authors.lastname: Almeida
authors.affiliation: Laboratório de Tecnologia Bioquímica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Alexandre José
authors.initials: AJ
authors.lastname: Macedo
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: Tiana
authors.initials: T
authors.lastname: Tasca
mesh_terms: D000243:Adenosine Deaminase / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development
chemical_list: D007502:Iron Chelating Agents; D015800:Protozoan Proteins; D000243:Adenosine Deaminase