title: SERUM CONCENTRATION OF NITRIC OXIDE IN WOMEN SMOKERS AND NONSMOKERS WITH OVERWEIGHT.
abstract: OBJECTIVE
  to evaluate the serum concentration of NO in overweight women, smokers (SG) and nonsmokers (NSG).
  
  
  METHODS
  blood samples from smokers (n = 20) and nonsmokers (n = 18) were collected to obtain serum, and stored at -80 °C until analysis. NO was assessed by measuring total nitrite, determined by Greiss method. It was adopted as reference 24.4 μmol/L, mean value found in a study with healthy subjects without excess weight. We used the Student t test to compare the means of age and waist circumference, and the Mann-Whitney U test to compare the median of concentrations of nitrite, number of cigarettes/day and Body Mass Index. We adopted a significance level of p < 0.05.
  
  
  RESULTS
  the median nitrite in SG was 16.53 (2.79 - 69.72) μmol/L, whereas in NSG was 10.85 (1.44 - 43.25) μmol/L (p = 0.028). BMI median value to SG and NSG, was respectively 29.50 (25.00 - 38.14) kg/m2 and 30.68 (25.10 - 36.98) kg/m2 (p = 0.530), being classified as overweight. The data showing that the average nitrite was below the estimated value for healthy individuals.
  
  
  CONCLUSIONS
  the results indicate a decrease of NO metabolites in women with excess weight, independently of being smoker. Despite the significant difference found between groups, these women had values well below the reference value of NO for healthy women. Therefore, it seems that smoking does not interfere in nitrite levels in patients already compromised by obesity.
authors.affiliation: Post graduate program in Brazilian health, Faculty of Medicine, Federal University of Juiz de Fora (UFJF). University City, Juiz de Fora, MG.. ketakeulen@gmail.com.
authors.forename: Henriqueta
authors.initials: H
authors.lastname: Vieira van Keulen
authors.affiliation: Post graduate program in health and nutrition, Federal University of Ouro Preto (UFOP), Campus Morros do Cruzeiro, s/n, Bauxita, Ouro Preto, MG.. arthurdasilvagomes@gmail.com.
authors.forename: Arthur
authors.initials: A
authors.lastname: da Silva Gomes
authors.affiliation: Post graduate program in Brazilian health, Faculty of Medicine, Federal University of Juiz de Fora (UFJF). University City, Juiz de Fora, MG.. maylanut@yahoo.com.br.
authors.forename: Mayla
authors.initials: M
authors.lastname: Cardoso Fernandes Toffolo
authors.affiliation: Institute of Biological Sciences - Cell therapy laboratory and immunology applied, Federal University of Juiz de Fora (UFJF). University City, Juiz de Fora, MG.. erickestevesdeoliveira@gmail.com.
authors.forename: Erick
authors.initials: E
authors.lastname: Esteves Oliveira
authors.affiliation: Institute of Biological Sciences - Cell therapy laboratory and immunology applied, Federal University of Juiz de Fora (UFJF). University City, Juiz de Fora, MG.. luan_jfmg2@yahoo.com.br.
authors.forename: Luan
authors.initials: L
authors.lastname: Cristian da Silva
authors.affiliation: Institute of Biological Sciences - Department of Nutrition, Federal University of Juiz de Fora, University City, Juiz de Fora, MG. Brazil.. scpotentedutra@yahoo.com.br.
authors.forename: Sheila
authors.initials: S
authors.lastname: Cristina Potente Dutra Luquetti
authors.affiliation: Institute of Biological Sciences - Cell therapy laboratory and immunology applied, Federal University of Juiz de Fora (UFJF). University City, Juiz de Fora, MG.. anapaula@ufjf.edu.br.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ferreira
authors.affiliation: Institute of Biological Sciences - Department of Nutrition, Federal University of Juiz de Fora, University City, Juiz de Fora, MG. Brazil.. aline.aguiar@ufjf.edu.br.
authors.forename: Aline
authors.initials: A
authors.lastname: Silva de Aguiar
mesh_terms: D000328:Adult; D000368:Aged; D015992:Body Mass Index; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D009765:Obesity / Q000097:blood; D050177:Overweight / Q000097:blood*; D012907:Smoking / Q000097:blood*; D055815:Young Adult
chemical_list: D009569:Nitric Oxide