title: Pros and cons of VP1-specific maternal IgG for the protection of Enterovirus 71 infection.
abstract: Enterovirus 71 (EV71) causes hand, foot, and mouth diseases and can result in severe neurological disorders when it infects the central nervous system. Thus, there is a need for the development of effective vaccines against EV71 infection. Here we report that viral capsid protein 1 (VP1), one of the main capsid proteins of EV71, efficiently elicited VP1-specific immunoglobulin G (IgG) in the serum of mice immunized with recombinant VP1. The VP1-specific IgG produced in female mice was efficiently transferred to their offspring, conferring protection against EV71 infection immediately after birth. VP1-specific antibody can neutralize EV71 infection and protect host cells. VP1-specific maternal IgG in offspring was maintained for over 6 months. However, the pre-existence of VP1-specific maternal IgG interfered with the production of VP1-specific IgG antibody secreting cells by active immunization in offspring. Therefore, although our results showed the potential for VP1-specific maternal IgG protection against EV71 in neonatal mice, other strategies must be developed to overcome the hindrance of maternal IgG in active immunization. In this study, we developed an effective and feasible animal model to evaluate the protective efficacy of humoral immunity against EV71 infection using a maternal immunity concept.
authors.affiliation: Laboratory of Microbiology, College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Young-In
authors.initials: YI
authors.lastname: Kim
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jae-Hyoung
authors.initials: JH
authors.lastname: Song
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Bo-Eun
authors.initials: BE
authors.lastname: Kwon
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Ha-Neul
authors.initials: HN
authors.lastname: Kim
authors.affiliation: Laboratory of Microbiology, College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea; Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of Korea.
authors.forename: Min-Duk
authors.initials: MD
authors.lastname: Seo
authors.affiliation: Department of Microbiology, Chungcheongnam-Do Institute of Health and Environment Research, Daejeon 300-801, Republic of Korea.
authors.forename: KwiSung
authors.initials: K
authors.lastname: Park
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Cheongju 361-951, Republic of Korea.
authors.forename: SangWon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention, Cheongju 361-951, Republic of Korea.
authors.forename: Sang-Gu
authors.initials: SG
authors.lastname: Yeo
authors.affiliation: Mucosal Immunology Laboratory, Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea.
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Kweon
authors.affiliation: Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: hjko@kangwon.ac.kr.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Ko
authors.affiliation: Laboratory of Microbiology, College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea. Electronic address: sychang@ajou.ac.kr.
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Chang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology*; D004769:Enterovirus Infections / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D056724:Immunity, Humoral; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D015678:Viral Structural Proteins; D014765:Viral Vaccines
keywords: Enterovirus 71; Maternal IgG; Pre-existing immunity; Protective immunity; VP1