title: Process Performance and Bacterial Community Structure Under Increasing Influent Disturbances in a Membrane-Aerated Biofilm Reactor.
abstract: The membrane-aerated biofilm reactor (MABR) is a promising municipal wastewater treatment process. In this study, two cross-flow MABRs were constructed to explore the carbon and nitrogen removal performance and bacterial succession, along with changes of influent loading shock comprising flow velocity, COD, and NH4-N concentrations. Redundancy analysis revealed that the function of high flow velocity was mainly embodied in facilitating contaminants diffusion and biosorption rather than the success of overall bacterial populations (p > 0.05). In contrast, the influent NH4-N concentration contributed most to the variance of reactor efficiency and community structure (p < 0.05). Pyrosequencing results showed that Anaerolineae, and Beta- and Alphaproteobacteria were the dominant groups in biofilms for COD and NH4-N removal. Among the identified genera, Nitrosomonas and Nitrospira were the main nitrifiers, and Hyphomicrobium, Hydrogenophaga, and Rhodobacter were the key denitrifiers. Meanwhile, principal component analysis indicated that bacterial shift in MABR was probably the combination of stochastic and deterministic processes.
authors.affiliation: Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R. China.|State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, P.R. China.|Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, P.R. China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, P.R. China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Tian
authors.affiliation: Institute of New Energy and Low-Carbon Technology, Sichuan University, Chengdu 610000, P.R. China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Yan
authors.affiliation: College of Food Science and Biotechnology, Food Safety Key Laboratory of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, P.R. China.
authors.forename: Yuewen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R. China.|State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, P.R. China.|Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, P.R. China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, P.R. China.
authors.forename: Baoan
authors.initials: B
authors.lastname: Li
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism; D059013:Microbial Consortia / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism; D009606:Nitrosomonas / Q000235:genetics / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D025341:Principal Component Analysis; D014865:Waste Disposal, Fluid / Q000592:standards; D062065:Wastewater / Q000737:chemistry
chemical_list: D002244:Carbon; D009584:Nitrogen; D010100:Oxygen; D062065:Wastewater
keywords: MABR; bacterial community assembly; biofilm; flow velocity