title: Microfabrication of Cell-Laden Hydrogels for Engineering Mineralized and Load Bearing Tissues.
abstract: Microengineering technologies and advanced biomaterials have extensive applications in the field of regenerative medicine. In this chapter, we review the integration of microfabrication techniques and hydrogel-based biomaterials in the field of dental, bone, and cartilage tissue engineering. We primarily discuss the major features that make hydrogels attractive candidates to mimic extracellular matrix (ECM), and we consider the benefits of three-dimensional (3D) culture systems for tissue engineering applications. We then focus on the fundamental principles of microfabrication techniques including photolithography, soft lithography and bioprinting approaches. Lastly, we summarize recent research on microengineering cell-laden hydrogel constructs for dental, bone and cartilage regeneration, and discuss future applications of microfabrication techniques for load-bearing tissue engineering.
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, New Research Building, 458H, 77 Avenue Louis Pastuer, Boston, MA, USA.|Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: Chia-Cheng
authors.initials: CC
authors.lastname: Li
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: Kharaziha
authors.affiliation: Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Min
authors.affiliation: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, New Research Building, 458H, 77 Avenue Louis Pastuer, Boston, MA, USA. maas@genetics.med.harvard.edu.
authors.forename: Richard
authors.initials: R
authors.lastname: Maas
authors.affiliation: Harrington Department of Biomedical Engineering, School of Biological and Health Systems Engineering, Arizona State University, ECG 334A, 501 E. Tyler Mall, Tempe, AZ, 85287, USA. mnikkhah@asu.edu.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Nikkhah
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism*; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002356:Cartilage / Q000166:cytology / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism*; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000166:cytology / Q000502:physiology; D016474:Weight-Bearing
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D008903:Minerals
keywords: Bioprinting; Bone; Cartilage; Dentistry; Hydrogel; Load-bearing tissue engineering; Microfabrication; Photolithography; Soft lithography