title: Iron metabolism following intravenous transfusion with stored versus fresh autologous erythrocyte concentrate in healthy dogs.
abstract: OBJECTIVE To determine effects of IV transfusion with fresh (3-day-old) or stored (35-day-old) autologous erythrocyte concentrate on serum labile iron concentration, iron-binding capacity, and protein interaction with iron in dogs. ANIMALS 10 random-source healthy dogs. PROCEDURES Dogs were randomly assigned to receive autologous erythrocyte concentrate stored for 3 days (n = 5) or 35 days (5). One unit of whole blood was collected from each dog, and erythrocyte concentrates were prepared and stored as assigned. After erythrocyte storage, IV transfusion was performed, with dogs receiving their own erythrocyte concentrate. Blood samples were collected from each dog before and 5, 9, 24, 48, and 72 hours after transfusion. Serum was harvested for measurement of total iron, labile iron, transferrin, ferritin, hemoglobin, and haptoglobin concentrations. RESULTS For dogs that received fresh erythrocytes, serum concentrations of the various analytes largely remained unchanged after transfusion. For dogs that received stored erythrocytes, serum concentrations of total iron, labile iron, hemoglobin, and ferritin increased markedly and serum concentrations of transferrin and haptoglobin decreased after transfusion. CONCLUSIONS AND CLINICAL RELEVANCE Transfusion with autologous erythrocyte concentrate stored for 35 days resulted in evidence of intravascular hemolysis in healthy dogs. The associated marked increases in circulating concentrations of free iron and hemoglobin have the potential to adversely affect transfusion recipients.
authors.forename: Virginie A
authors.initials: VA
authors.lastname: Wurlod
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Smith
authors.forename: Maureen A
authors.initials: MA
authors.lastname: McMichael
authors.forename: Mauria
authors.initials: M
authors.lastname: O'Brien
authors.forename: Jennifer
authors.initials: J
authors.lastname: Herring
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Swanson
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods*; D004285:Dogs; D017707:Erythrocyte Transfusion / Q000662:veterinary*; D004912:Erythrocytes / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D006242:Haptoglobins / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000097:blood / Q000378:metabolism*; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism
chemical_list: D006242:Haptoglobins; D006454:Hemoglobins; D014168:Transferrin; D005293:Ferritins; D007501:Iron