title: Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo.
abstract: In zebrafish embryos, factors involved in both axis induction and primordial germ cell (PGC) development are localized to the vegetal pole of the egg. However, upon egg activation axis induction factors experience an asymmetric off-center shift whereas PGC factors undergo symmetric animally-directed movement. We examined the spatial relationship between the proposed dorsal genes wnt8a and grip2a and the PGC factor dazl at the vegetal cortex. We find that RNAs for these genes localize to different cortical depths, with the RNA for the PGC factor dazl at a deeper cortical level than those for axis-inducing factors. In addition, and in contrast to the role of microtubules in the long-range transport of dorsal determinants, we find that germ line determinant transport depends on the actin cytoskeleton. Our results support a model in which vegetal cortex differential RNA transport behavior is facilitated by RNA localization along cortical depth and differential coupling to cortical transport.
authors.affiliation: a Laboratory of Genetics; University of Wisconsin - Madison ; Madison , WI USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Welch
authors.affiliation: a Laboratory of Genetics; University of Wisconsin - Madison ; Madison , WI USA.
authors.forename: Francisco
authors.initials: F
authors.lastname: Pelegri
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D001692:Biological Transport; D019521:Body Patterning / Q000235:genetics*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005260:Female; D005306:Fertilization; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018091:Receptors, AMPA / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D053778:Thiazolidines / Q000494:pharmacology; D050257:Tubulin Modulators / Q000494:pharmacology; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism; D015053:Zygote / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C546118:DAZL protein, zebrafish; C000610114:Grip2a protein, zebrafish; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018091:Receptors, AMPA; D053778:Thiazolidines; D050257:Tubulin Modulators; D051153:Wnt Proteins; D029961:Zebrafish Proteins; C094521:wnt8a protein, zebrafish; D015739:Nocodazole; C037067:latrunculin A
keywords: RNA localization; axis induction; cortical rotation; embryo; germ cell determination; germ plasm; vegetal cortex; zebrafish