title: Field evidence of reproduction impairment through sperm DNA damage in the fish nase (Chondrostoma nasus) in anthropized hydrosystems.
abstract: This work aims to explore in the field the relationship between the integrity of sperm DNA and the quality of offspring as a possible cause of the decline of a feral fish population through reproduction impairment. Mature nase (Chondrostoma nasus) were caught during the breeding season in three locations (A-C) of the Rhône River basin and gametes collected by stripping. Sampling locations were chosen according to the following gradient of contamination due to human activities on the watershed: A≤B<C. Samples of a pool of collected oocytes were fertilized with the sperm of individual males and then incubated individually back in the lab to study embryo-larval development as well as using sperm samples to assess DNA integrity. Genetic analysis clearly showed the absence of a difference in genetic structure between the three studied C. nasus populations from the Rhône basin. Sperm DNA integrity was significantly lower in males from station C compared to other ones while sperm biochemical characteristics and fertilization rate remained almost unchanged whatever the station. Mortality and abnormality rates measured at both hatching and at the end of yolk sac resorption stages followed the same trend as the sperm DNA damage, demonstrating an impact of river water quality on nase fitness through a loss of sperm DNA integrity. Since the level of both abnormalities and mortality measured in offspring of fish caught in the most contaminated area reached high values up to 15% and 80%, respectively, the hypothesis that the observed nase decline in Rhône River stemming through selection forces can be put forward.
authors.affiliation: Université de Lyon, USC INRA 1369, UMR CNRS 5023 LEHNA, ENTPE, Rue Maurice Audin, F-69518 Vaulx en Velin, France. Electronic address: alain.devaux@entpe.fr.
authors.forename: Alain
authors.initials: A
authors.lastname: Devaux
authors.affiliation: Université de Lyon, USC INRA 1369, UMR CNRS 5023 LEHNA, ENTPE, Rue Maurice Audin, F-69518 Vaulx en Velin, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bony
authors.affiliation: Université de Lyon, UMR CNRS 5023 LEHNA, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Plenet
authors.affiliation: Université de Lyon, UMR CNRS 5023 LEHNA, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Sagnes
authors.affiliation: Université de Lyon, UMR CNRS 5023 LEHNA, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
authors.forename: Samuel
authors.initials: S
authors.lastname: Segura
authors.affiliation: Université de Lyon, USC INRA 1369, UMR CNRS 5023 LEHNA, ENTPE, Rue Maurice Audin, F-69518 Vaulx en Velin, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Suaire
authors.affiliation: Université de Lyon, UMR CNRS 5023 LEHNA, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Novak
authors.affiliation: Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, Aix Marseille Université, CNRS, IRD, Avignon Université, centre Saint Charles, 3 place Victor Hugo, F-13331 Marseille, France.
authors.forename: André
authors.initials: A
authors.lastname: Gilles
authors.affiliation: Université de Lyon, UMR CNRS 5023 LEHNA, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Olivier
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000235:genetics / Q000502:physiology*; D004249:DNA Damage; D004625:Embryo, Nonmammalian; D008297:Male; D012098:Reproduction / Q000187:drug effects*; D045483:Rivers / Q000737:chemistry; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical
keywords: Fish population decline; Fitness; Genotoxicity; Pollutant; Sperm