title: A new method for monitoring the extracellular proteolytic activity of wine yeasts during alcoholic fermentation of grape must.
abstract: The existing methods for testing proteolytic activity are time consuming, quite difficult to perform, and do not allow real-time monitoring. Proteases have attracted considerable interest in winemaking and some yeast species naturally present in grape must, such as Metschnikowia pulcherrima, are capable of expressing this activity. In this study, a new test is proposed for measuring proteolytic activity directly in fermenting grape must, using azocasein, a chromogenic substrate. Several yeast strains were tested and differences in proteolytic activity were observed. Moreover, analysis of grape must proteins in wines revealed that protease secreted by Metschnikowia strains may be active against wine proteins.
authors.affiliation: Univ. de Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33140 Villenave d'Ornon cedex, France; BioLaffort, 126 Quai de la Souys, 33100 Bordeaux, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Chasseriaud
authors.affiliation: Univ. de Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33140 Villenave d'Ornon cedex, France; INRA, ISVV, USC 1219 Œnologie, 33140 Villenave d'Ornon cedex, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Miot-Sertier
authors.affiliation: BioLaffort, 126 Quai de la Souys, 33100 Bordeaux, France.
authors.forename: Joana
authors.initials: J
authors.lastname: Coulon
authors.affiliation: BioLaffort, 126 Quai de la Souys, 33100 Bordeaux, France.
authors.forename: Nerea
authors.initials: N
authors.lastname: Iturmendi
authors.affiliation: BioLaffort, 126 Quai de la Souys, 33100 Bordeaux, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Moine
authors.affiliation: Univ. de Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33140 Villenave d'Ornon cedex, France.
authors.forename: Warren
authors.initials: W
authors.lastname: Albertin
authors.affiliation: Univ. de Bordeaux, ISVV, EA 4577, Unité de Recherche Œnologie, 33140 Villenave d'Ornon cedex, France. Electronic address: laura.chasseriaud@u-bordeaux.fr.
authors.forename: Marina
authors.initials: M
authors.lastname: Bely
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007700:Kinetics; D055163:Metschnikowia / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism*; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000032:analysis / Q000382:microbiology*; D015003:Yeasts / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D000431:Ethanol; D010447:Peptide Hydrolases
keywords: Alcoholic fermentation; Azocasein; Metschnikowia spp.; Proteolytic activity; Wine