title: Bacillus licheniformis Isolated from Traditional Korean Food Resources Enhances the Longevity of Caenorhabditis elegans through Serotonin Signaling.
abstract: In this study, we investigated potentially probiotic Bacillus licheniformis strains isolated from traditional Korean food sources for ability to enhance longevity using the nematode Caenorhabditis elegans as a simple in vivo animal model. We first investigated whether B. licheniformis strains were capable of modulating the lifespan of C. elegans. Among the tested strains, preconditioning with four B. licheniformis strains significantly enhanced the longevity of C. elegans. Unexpectedly, plate counting and transmission electron microscopy (TEM) results indicated that B. licheniformis strains were not more highly attached to the C. elegans intestine compared with Escherichia coli OP50 or Lactobacillus rhamnosus GG controls. In addition, qRT-PCR and an aging assay with mutant worms showed that the conditioning of B. licheniformis strain 141 directly influenced genes associated with serotonin signaling in nematodes, including tph-1 (tryptophan hydroxylase), bas-1 (serotonin- and dopamine-synthetic aromatic amino acid decarboxylase), mod-1 (serotonin-gated chloride channel), ser-1, and ser-7 (serotonin receptors) during C. elegans aging. Our findings suggest that B. licheniformis strain 141, which is isolated from traditional Korean foods, is a probiotic generally recognized as safe (GRAS) strain that enhances the lifespan of C. elegans via host serotonin signaling.
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University , Jeonju 561-756, Korea.
authors.forename: Mi Ri
authors.initials: MR
authors.lastname: Park
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University , Jeonju 561-756, Korea.
authors.forename: Sangnam
authors.initials: S
authors.lastname: Oh
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University , Jeonju 561-756, Korea.
authors.forename: Seok Jun
authors.initials: SJ
authors.lastname: Son
authors.affiliation: Korea Food Research Institute , Seongnam-si, Gyeonggi-do 463-746, Korea.
authors.forename: Dong-June
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Division of Animal Science, Chonnam National University , Gwangju 500-757, Korea.
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.affiliation: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University , 136-701 Seoul, Korea.
authors.forename: Sae Hun
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Microbial Institute for Fermentation Industry , Sunchang, Jeonbuk 595-804, Republic of Korea.
authors.forename: Do-Youn
authors.initials: DY
authors.lastname: Jeong
authors.affiliation: R&D Center, Seoul Dairy Cooperative , Ansan, Gyeonggi-do 425-839, South Korea.
authors.forename: Nam Su
authors.initials: NS
authors.lastname: Oh
authors.affiliation: Department of Applied Chemistry, Hanyang University , ERICA Campus, Ansan, Gyeonggi-do 426-791, Korea.
authors.forename: Youngbok
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Animal Science and Biotechnology, Chungnam National University , Daejeon 305-764, Korea.
authors.forename: Minho
authors.initials: M
authors.lastname: Song
authors.affiliation: BK21 Plus Graduate Program, Department of Animal Science and Institute Agricultural Science & Technology, Chonbuk National University , Jeonju 561-756, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D008136:Longevity; D008297:Male; D019936:Probiotics / Q000008:administration & dosage / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D015398:Signal Transduction; D014675:Vegetables / Q000382:microbiology*
chemical_list: D029742:Caenorhabditis elegans Proteins; D012701:Serotonin
keywords: Bacillus licheniformis; Caenorhabditis elegans; Korean traditional foods; antiaging; serotonin signaling