title: The association and aggregation of the metamorphic chemokine lymphotactin with fondaparinux: from nm molecular complexes to μm molecular assemblies.
abstract: Transmission electron microscopy, mass spectrometry, and drift tube ion mobility-mass spectrometry are used to study the assemblies formed by the metamorphic chemokine lymphotactin in the presence of a model pentameric glycosaminoglycan, fondaparinux. This combination of techniques delineates significant differences in the complexes observed for two forms of the full length protein as well as a truncated form, without the intrinsically disordered C-terminal tail, over a length scale from few nm to μm assemblies.
authors.affiliation: School of Chemistry, University of Edinburgh, West Mains Road, EH8 3JJ, Edinburg, UK.
authors.forename: Sophie R
authors.initials: SR
authors.lastname: Harvey
authors.forename: Cait E
authors.initials: CE
authors.lastname: MacPhee
authors.forename: Brian F
authors.initials: BF
authors.lastname: Volkman
authors.forename: Perdita E
authors.initials: PE
authors.lastname: Barran
mesh_terms: D019744:Chemokines, C / Q000737:chemistry / Q000378:metabolism*; D000077425:Fondaparinux; D008222:Lymphokines / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D066329:Protein Aggregates; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D012795:Sialoglycoproteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019744:Chemokines, C; D008222:Lymphokines; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011134:Polysaccharides; D066329:Protein Aggregates; D012795:Sialoglycoproteins; C018997:lymphotactin; D000077425:Fondaparinux