title: Anti-Tumor Effect of Pinus massoniana Bark Proanthocyanidins on Ovarian Cancer through Induction of Cell Apoptosis and Inhibition of Cell Migration.
abstract: Pinus massoniana bark proanthocyanidins (PMBPs), an active component isolated from Pinus massoniana bark, has been reported to possess a wide range of biochemical properties. Here, we investigated the anti-tumor effect of PMBPs on ovarian cancer. The results indicated that PMBPs significantly reduced the growth of ovarian cancer cells and induced dose-dependent apoptosis. The underlying mechanisms involved were elucidated to include the loss of mitochondrial membrane potential, down-regulation of the anti-apoptotic protein Bcl-2 and the activation of Caspase 3/9, suggesting that PMBPs triggered apoptosis through activation of mitochondria-associated apoptotic pathway. In addition, wound healing and transwell chamber assays revealed that PMBPs could suppress migration and invasion of ovarian cancer cells. PMBPs dramatically inhibited MMP-9 activity and expression, blocked the activity of NFκB and the activation of ERK1/2 and p38 MAPK. Our findings suggest that PMBPs has the potential to be developed as an anti-tumor drug for ovarian cancer treatment and/ or disease management.
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Dachang
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.|Department of Pharmaceutical Sciences, University of South Florida, Tampa, United States of America.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Owusu
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Ershao
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D044945:Proanthocyanidins / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D044945:Proanthocyanidins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D048051:p38 Mitogen-Activated Protein Kinases; D053148:Caspase 3; D053453:Caspase 9