title: Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages.
abstract: The activation of murine peritoneal macrophages by Ganoderma lucidum polysaccharides liposomes (GLPL) was investigated in vitro. After treatment with GLPL, the changes of the nitric oxide (NO) secretion and iNOS (inducible nitric oxide synthase) activity were evaluated. The results showed that NO production and iNOS activity of macrophages were enhanced compared to GLP and BL group. In addition, both the phagocytic activity and levels of cytokines IL-1β, TNF-α and IFN-γ were enhanced in the peritoneal macrophages of mice by stimulation of GLPL. The expression of the major histocompatibility complex class II molecule (MHC II) on the surface of peritoneal macrophages significantly increased. These indicated that GLPL could enhance the activation of peritoneal macrophages and their potential for use as a delivery system of GLP.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Zhenguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Ruonan
authors.initials: R
authors.lastname: Bo
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Luo
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Yale
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism
chemical_list: D016207:Cytokines; D062610:Fungal Polysaccharides; D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II
keywords: Ganoderma lucidum polysaccharides liposome; MHC II; Peritoneal macrophages; Phagocytosis