title: CD99 regulates CXCL12-induced chemotaxis of human plasma cells.
abstract: Migration of plasma cells (PCs) is crucial for the control of PC survival and antibody production and is controlled by chemokines, most importantly by CXCL12. This study investigated the role of CD99 in CXCL12-induced PC migration. Among B cell subsets in the tonsils, CD99 expression was highest in PCs. CD99 expression increased during in vitro differentiation of germinal center B cells and was highest in PCs. CD99 engagement reduced chemotactic migration of PCs toward CXCL12 and reduced extracellular signal-regulated kinase (ERK) activation by CXCL12. An ERK inhibitor reduced CXCL12-mediated chemotactic migration, which suggests that ERK has a critical role in migration. CD99 engagement did not influence apoptosis, differentiation, or antibody secretion of PCs. We propose a novel role of CD99 in PCs that suppresses ERK activation and chemotactic migration of these cells.
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Minchan
authors.initials: M
authors.lastname: Gil
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Hyo-Kyung
authors.initials: HK
authors.lastname: Pak
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: A-Neum
authors.initials: AN
authors.lastname: Lee
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Seo-Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Roh
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Hyunji
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea.
authors.forename: Yoo-Sam
authors.initials: YS
authors.lastname: Chung
authors.affiliation: Cell Dysfunction Research Center, University of Ulsan College of Medicine, Seoul, South Korea; Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea; Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: csikpark@amc.seoul.kr.
authors.forename: Chan-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D000071443:12E7 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods; D018858:Germinal Center / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007739:L Cells; D051379:Mice; D010950:Plasma Cells / Q000187:drug effects* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D054377:Chemokine CXCL12; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: CD99; Extracellular signal-regulated kinase; Migration; Plasma cell