title: Fixed single-cell transcriptomic characterization of human radial glial diversity.
abstract: The diverse progenitors that give rise to the human neocortex have been difficult to characterize because progenitors, particularly radial glia (RG), are rare and are defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed Fixed and Recovered Intact Single-cell RNA (FRISCR), a method for profiling the transcriptomes of individual fixed, stained and sorted cells. Using FRISCR, we profiled primary human RG that constitute only 1% of the midgestation cortex and classified them as ventricular zone-enriched RG (vRG) that express ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that express HOPX. Our study identified vRG and oRG markers and molecular profiles, an essential step for understanding human neocortical progenitor development. FRISCR allows targeted single-cell profiling of any tissues that lack live-cell markers.
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Elliot R
authors.identifier: http://orcid.org/0000-0001-9092-8678
authors.initials: ER
authors.lastname: Thomsen
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: John K
authors.initials: JK
authors.lastname: Mich
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Zizhen
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Hodge
authors.affiliation: Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Adele M
authors.initials: AM
authors.lastname: Doyle
authors.affiliation: Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Sumin
authors.initials: S
authors.lastname: Jang
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Soraya I
authors.initials: SI
authors.lastname: Shehata
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Angelique M
authors.initials: AM
authors.lastname: Nelson
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Nadiya V
authors.initials: NV
authors.lastname: Shapovalova
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.
authors.forename: Boaz P
authors.initials: BP
authors.lastname: Levi
authors.affiliation: Allen Institute for Brain Science, Seattle, Washington, USA.|Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA.|School of Engineering and Applied Science, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Sharad
authors.initials: S
authors.lastname: Ramanathan
mesh_terms: D001921:Brain / Q000166:cytology*; D006801:Humans; D009457:Neuroglia / Q000166:cytology*; D059010:Single-Cell Analysis; D059467:Transcriptome