title: Evaluation of less invasive methods to assess fatty acids from phospholipid fraction: cheek cell and capillary blood sampling.
abstract: Plasma is the most commonly employed matrix for analyzing fatty acids (FAs), but its extraction is not well accepted in the infant population. The objectives of this study were to evaluate cheek cells and capillary blood as alternatives to plasma sampling for FA analysis and to standardize the methodology. Samples were obtained from 20 children who underwent lipid extraction, phospholipid isolation by Solid Phase Extraction (SPE) in a 96-well plate, methylation, and analysis by fast gas chromatography (GC). A positive correlation was found for most of the FAs, especially long-chain polyunsaturated fatty acids (LC-PUFAs), in cheek cells and capillary blood versus plasma samples (r = 0.32-0.99). No differences were found in the levels of n-6: n-3 PUFA and n-6: n-3 LC-PUFA ratios between cheek cells and capillary blood. These two proposed samples can therefore be used as alternatives to plasma sampling for phospholipid FA analysis, especially LC-PUFAs.
authors.affiliation: a Department of Nutrition and Food Science , Faculty of Pharmacy, University of Barcelona , Barcelona , Spain .
authors.forename: Andrea
authors.initials: A
authors.lastname: de la Garza Puentes
authors.affiliation: a Department of Nutrition and Food Science , Faculty of Pharmacy, University of Barcelona , Barcelona , Spain .|b CIBER Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III , Madrid , Spain , and.
authors.forename: Rosa
authors.initials: R
authors.lastname: Montes Goyanes
authors.affiliation: a Department of Nutrition and Food Science , Faculty of Pharmacy, University of Barcelona , Barcelona , Spain .
authors.forename: Aida Maribel
authors.initials: AM
authors.lastname: Chisaguano Tonato
authors.affiliation: a Department of Nutrition and Food Science , Faculty of Pharmacy, University of Barcelona , Barcelona , Spain .|b CIBER Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III , Madrid , Spain , and.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Castellote
authors.affiliation: c EURISTIKOS Excellence Centre for Pediatric Research, University of Granada , Granada , Spain.
authors.forename: Rosario
authors.initials: R
authors.lastname: Moreno-Torres
authors.affiliation: c EURISTIKOS Excellence Centre for Pediatric Research, University of Granada , Granada , Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Campoy Folgoso
authors.affiliation: a Department of Nutrition and Food Science , Faculty of Pharmacy, University of Barcelona , Barcelona , Spain .|b CIBER Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III , Madrid , Spain , and.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: López-Sabater
mesh_terms: D002610:Cheek; D002648:Child; D002849:Chromatography, Gas; D015525:Fatty Acids, Omega-3 / Q000097:blood*; D043371:Fatty Acids, Omega-6 / Q000097:blood*; D006801:Humans; D010743:Phospholipids / Q000032:analysis*; D010949:Plasma / Q000737:chemistry; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D010743:Phospholipids
keywords: Buccal mucosal cells; fingertip blood; infants; phospholipids; plasma