title: Co-expression of G2-EPSPS and glyphosate acetyltransferase GAT genes conferring high tolerance to glyphosate in soybean.
abstract: Glyphosate is a widely used non-selective herbicide with broad spectrum of weed control around the world. At present, most of the commercial glyphosate tolerant soybeans utilize glyphosate tolerant gene CP4-EPSPS or glyphosate acetyltransferase gene GAT separately. In this study, both glyphosate tolerant gene G2-EPSPS and glyphosate degraded gene GAT were co-transferred into soybean and transgenic plants showed high tolerance to glyphosate. Molecular analysis including PCR, Sothern blot, qRT-PCR, and Western blot revealed that target genes have been integrated into genome and expressed effectively at both mRNA and protein levels. Furthermore, the glyphosate tolerance analysis showed that no typical symptom was observed when compared with a glyphosate tolerant line HJ06-698 derived from GR1 transgenic soybean even at fourfold labeled rate of Roundup. Chlorophyll and shikimic acid content analysis of transgenic plant also revealed that these two indexes were not significantly altered after glyphosate application. These results indicated that co-expression of G2-EPSPS and GAT conferred high tolerance to the herbicide glyphosate in soybean. Therefore, combination of tolerant and degraded genes provides a new strategy for developing glyphosate tolerant transgenic crops.
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China ; College of Agriculture, Northeast Agricultural University , Harbin, China.
authors.forename: Bingfu
authors.initials: B
authors.lastname: Guo
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China ; College of Agriculture, Northeast Agricultural University , Harbin, China.
authors.forename: Huilong
authors.initials: H
authors.lastname: Hong
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Longguo
authors.initials: L
authors.lastname: Jin
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Ru-Zhen
authors.initials: RZ
authors.lastname: Chang
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Qiu
keywords: G2-EPSPS; GAT; chlorophyll content; glyphosate tolerance; shikimic acid; transgenic soybean