title: Optimised extraction of Erythronium sibiricum bulb polysaccharides and evaluation of their bioactivities.
abstract: In this study, the extraction of Erythronium sibiricum bulb polysaccharides (ESBP) through hot water decoction was optimised using response surface methodology (RSM) and a three-level, four-factor Box-Behnken design. The optimum extraction conditions were as follows: extraction time of 4.28h, extraction temperature of 90°C, ratio of liquid to raw material of 37 mL/g and extraction cycle number of three. The experimental yield (37.25%±0.17%) agreed with the predicted value of the RSM model (37.465%). Preliminary ESBP characterisation was conducted through physicochemical analysis. Biological activity test results showed that ESBP exhibited antioxidant activities and excellent anti-inflammatory and analgesic activities, indicating its potential as an anti-inflammatory and analgesic agent.
authors.affiliation: Pharmacy College of Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Chen
authors.affiliation: Pharmacy College of Xinjiang Medical University, Urumqi, 830011, China. Electronic address: renakasimu@vip.sina.com.
authors.forename: Rena
authors.initials: R
authors.lastname: Kasimu
authors.affiliation: Pharmacy College of Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Xie
authors.affiliation: College of Medical Engineering and Technology, Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Central Laboratory of Xinjiang Medical University, Urumqi, 830011, China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Ding
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D019668:Liliaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Biological activities; Erythronium sibiricum; Polysaccharide extraction