title: Modifying alginate with early embryonic extracellular matrix, laminin, and hyaluronic acid for adipose tissue engineering.
abstract: Extracellular matrix provides both mechanistic and chemical cues that can influence cellular behaviors such as adhesion, migration, proliferation, and differentiation. In this study, a new material, HA-L-Alg, was synthesized by linking developmentally essential ECM constituents hyaluronic acid (HA) and laminin(L) to alginate (Alg). The fabrication of HA-L-Alg was confirmed by FTIR spectroscopy, and it was used to form 3D cell-carrying beads. HA-L-Alg beads had a steady rate of degradation and retained 63.25% of mass after 9 weeks. HA-L-Alg beads showed biocompatibility comparable to beads formed by Alg-only with no obvious cytotoxic effect on the embedded 3T3-L1 preadipocytes. HA-L-Alg encapsulated 3T3-L1 cells were found to have a higher proliferation rate over those in Alg-only beads. These cells also showed better differentiation capacity after 2 weeks of adipogenic induction within HA-L-Alg beads. These results support that HA-L-Alg facilitated cell survival and proliferation, as well as stimulated and maintained cell differentiation. Our results suggest that HA-L-Alg has a great clinical potential to be used as stem cell carrier for adipose tissue engineering. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 669-677, 2016.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan.|Department of Plastic Surgery, Far Eastern Memorial Hospital, New Taipei City, 22060, Taiwan.
authors.forename: Yo-Shen
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Yen-Yu
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Hsueh
authors.affiliation: Department of Plastic Surgery, National Taiwan University Hospital, Taipei, 10051, Taiwan.
authors.forename: Hao-Chih
authors.initials: HC
authors.lastname: Tai
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, 10051, Taiwan.
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology*; D000464:Alginates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002135:Calcium-Binding Proteins; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005109:Extracellular Matrix / Q000378:metabolism*; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D008863:Microspheres; D047495:PPAR gamma / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D002135:Calcium-Binding Proteins; C512925:Dlk1 protein, mouse; D006603:Hexuronic Acids; D036341:Intercellular Signaling Peptides and Proteins; D007797:Laminin; D047495:PPAR gamma; D020723:Glucuronic Acid; D006820:Hyaluronic Acid
keywords: HA-L-Alg beads; adipose tissue engineering; embryonic; extracellular matrix