title: 1H, 13C, and 15N chemical shift assignments of cyanobacteriochrome NpR6012g4 in the green-absorbing photoproduct state.
abstract: Cyanobacteriochromes (CBCRs) are cyanobacterial photosensory proteins with a tetrapyrrole (bilin) chromophore that belong to the phytochrome superfamily. Like phytochromes, CBCRs photoconvert between two photostates with distinct spectral properties. NpR6012g4 from Nostoc punctiforme is a model system for widespread CBCRs with conserved red/green photocycles. Atomic-level structural information for the photoproduct state in this subfamily is not known. Here, we report NMR backbone chemical shift assignments of the light-activated state of NpR6012g4 (BMRB no. 26577) as a first step toward determining its atomic resolution structure.
authors.affiliation: Department of Chemistry, University of California, Davis, CA, 95616, USA.
authors.forename: Sunghyuk
authors.initials: S
authors.lastname: Lim
authors.affiliation: Department of Chemistry, University of California, Davis, CA, 95616, USA.
authors.forename: Qinhong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Rockwell
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
authors.forename: Shelley S
authors.initials: SS
authors.lastname: Martin
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA.
authors.forename: J Clark
authors.initials: JC
authors.lastname: Lagarias
authors.affiliation: Department of Chemistry, University of California, Davis, CA, 95616, USA. jbames@ucdavis.edu.
authors.forename: James B
authors.initials: JB
authors.lastname: Ames
mesh_terms: D065638:Absorption, Radiation; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D003116:Color; D046937:Nostoc; D019906:Nuclear Magnetic Resonance, Biomolecular; D020283:Photoreceptors, Microbial / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial
keywords: CBCR; Cyanobacteria; Cyanobacteriochrome; NMR; Photoreceptor; Phycocyanobilin; Phytochrome