title: Characterization of material properties of soft solid thin layers with acoustic radiation force and wave propagation.
abstract: Evaluation of tissue engineering constructs is performed by a series of different tests. In many cases it is important to match the mechanical properties of these constructs to those of native tissues. However, many mechanical testing methods are destructive in nature which increases cost for evaluation because of the need for additional samples reserved for these assessments. A wave propagation method is proposed for characterizing the shear elasticity of thin layers bounded by a rigid substrate and fluid-loading, similar to the configuration for many tissue engineering applications. An analytic wave propagation model was derived for this configuration and compared against finite element model simulations and numerical solutions from the software package Disperse. The results from the different models found very good agreement. Experiments were performed in tissue-mimicking gelatin phantoms with thicknesses of 1 and 4 mm and found that the wave propagation method could resolve the shear modulus with very good accuracy, no more than 4.10% error. This method could be used in tissue engineering applications to monitor tissue engineering construct maturation with a nondestructive wave propagation method to evaluate the shear modulus of a material.
authors.affiliation: Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55902, USA.
authors.forename: Matthew W
authors.initials: MW
authors.lastname: Urban
authors.affiliation: Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55902, USA.
authors.forename: Ivan Z
authors.initials: IZ
authors.lastname: Nenadic
authors.affiliation: Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55902, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Qiang
authors.affiliation: Cardiovascular Dynamics Research Group, Universidad Pontificia Bolivariana, Medellin, Colombia.
authors.forename: Miguel
authors.initials: M
authors.lastname: Bernal
authors.affiliation: Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55902, USA.
authors.forename: Shigao
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55902, USA.
authors.forename: James F
authors.initials: JF
authors.lastname: Greenleaf
mesh_terms: D003198:Computer Simulation; D004548:Elasticity; D020342:Finite Element Analysis; D005780:Gelatin; D005990:Glycerol; D008962:Models, Theoretical; D019047:Phantoms, Imaging; D033081:Shear Strength; D012984:Software; D023822:Tissue Engineering / Q000379:methods*; D000069453:Ultrasonic Waves
chemical_list: D005780:Gelatin; D005990:Glycerol