title: The role of interleukin-8 (CXCL8) and CXCR2 in acquired chemoresistance of human colorectal carcinoma cells HCT116.
abstract: Colorectal cancer is one of the most common malignant diseases and is a leading cause of cancer mortality in the Western world. Primary or acquired resistance to chemotherapeutic drugs is a common phenomenon which causes a failure in cancer treatment. A diverse range of molecular mechanisms has been implicated in drug resistance: DNA damage repair, alterations in drug metabolism, mutation of drug targets, increased rates of drug efflux, and activation of survival signaling pathways. The aim of this study was to investigate the expression of CXCL8-CXCR1/2 pathway, its impact on cell proliferation and cytokine expression in human colorectal carcinoma HCT116 cells, and their chemotherapy-resistant subline. We found that IL-1 alpha stimulates the production of CXCL8 through IL-1 receptor signaling. Our data indicate that CXCL8 is upregulated in chemoresistant subline of colorectal cancer cells HCT116, and modulation of CXCR2 pathway can be a target for proliferation inhibition of chemoresistant colorectal cancer cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania. daiva.dabkeviciene@gf.vu.lt.
authors.forename: Daiva
authors.identifier: http://orcid.org/0000-0003-3261-7622
authors.initials: D
authors.lastname: Dabkeviciene
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Violeta
authors.initials: V
authors.lastname: Jonusiene
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Vilmante
authors.initials: V
authors.lastname: Zitkute
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Egle
authors.initials: E
authors.lastname: Zalyte
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Pranas
authors.initials: P
authors.lastname: Grigaitis
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Vida
authors.initials: V
authors.lastname: Kirveliene
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Natural Sciences, Vilnius University, Vilnius, Lithuania.
authors.forename: Ausra
authors.initials: A
authors.lastname: Sasnauskiene
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects* / Q000502:physiology*; D005472:Fluorouracil / Q000494:pharmacology; D045325:HCT116 Cells; D006801:Humans; D053582:Interleukin-1alpha / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D009944:Organoplatinum Compounds / Q000494:pharmacology; D000077150:Oxaliplatin; D060888:Real-Time Polymerase Chain Reaction; D023063:Receptors, Interleukin-8B / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; C508606:CXCL8 protein, human; D053582:Interleukin-1alpha; D016209:Interleukin-8; D009944:Organoplatinum Compounds; D023063:Receptors, Interleukin-8B; D000077150:Oxaliplatin; D005472:Fluorouracil
keywords: 5-fluorouracil; CXCL8; CXCR2; Chemoresistance; Interleukin-1 alpha; Oxaliplatin