title: Sulfur decreases cadmium translocation and enhances cadmium tolerance by promoting sulfur assimilation and glutathione metabolism in Brassica chinensis L.
abstract: We investigated the ameliorative role of sulfur (S) in protecting plants against cadmium (Cd) toxicity by using two pakchoi (Brassica chinensis L.) cultivars with different Cd tolerance levels. The exposure of pakchoi seedlings to 100μM Cd inhibited plant growth, increased superoxide content, enhanced membrane lipid peroxidation, and induced Cd accumulation in the roots and shoots. Application of S to Cd-stressed plants alleviated Cd-induced oxidative stress by promoting the capacity of the ascorbate (AsA)-glutathione (GSH) cycle, enhanced S assimilation by increasing the activity of ATP sulfurylase (ATPS) and o-acetylserine(thiol)lyase (OASTL), and decreased Cd translocation from the roots to the shoots by enhancing phytochelatins (PCs) biosynthesis. Results suggested that S reversed Cd-induced growth inhibition and oxidative stress by restraining Cd translocation from the roots to the shoots and upregulating S assimilation and GSH metabolism, including the AsA-GSH cycle and PCs synthesis.
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Taishuai
authors.initials: T
authors.lastname: Liang
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Han
authors.initials: H
authors.lastname: Ding
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Jingquan
authors.initials: J
authors.lastname: Kang
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Hongxi
authors.initials: H
authors.lastname: Pang
authors.affiliation: College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: jinyinlv@nwsuaf.edu.cn.
authors.forename: Jinyin
authors.initials: J
authors.lastname: Lv
mesh_terms: D001205:Ascorbic Acid / Q000378:metabolism; D001937:Brassica / Q000187:drug effects* / Q000378:metabolism; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D003547:Cysteine Synthase / Q000378:metabolism; D004361:Drug Tolerance; D005978:Glutathione / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D012989:Soil Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D013430:Sulfate Adenylyltransferase / Q000378:metabolism; D013455:Sulfur / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D012989:Soil Pollutants; D002104:Cadmium; D013455:Sulfur; D003547:Cysteine Synthase; D013430:Sulfate Adenylyltransferase; D005978:Glutathione; D001205:Ascorbic Acid
keywords: Cadmium; Cd translocation; GSH metabolism; Pakchoi; Sulfur