title: Inhibition of acetylcholinesterase and cytochrome oxidase activity in Fasciola gigantica cercaria by phytoconstituents.
abstract: Fasciolosis is an important cattle and human disease caused by Fasciola hepatica and Fasciola gigantica. One of the possible methods to control this problem is to interrupt the life cycle of Fasciola by killing its larva (redia and cercaria) in host snail. Molecular identification of cercaria larva of F. gigantica was done by comparing the nucleotide sequencing with adult F. gigantica. It was noted that nucleotide sequencing of cercaria larva and adult F. gigantica were 99% same. Every month during the year 2011-2012, in vivo treatment with 60% of 4 h LC50 of phyto cercaricides citral, ferulic acid, umbelliferone, azadirachtin and allicin caused significant inhibition of acetylcholinesterase (AChE) and cytochrome oxidase activity in the treated cercaria larva of F. gigantica. Whereas, activity of both enzymes were not significantly altered in the nervous tissues of vector snail Lymnaea acuminata exposed to same treatments. Maximum reduction in AChE (1.35% of control in month of June) and cytochrome oxidase (3.71% of control in the month of July) activity were noted in the cercaria exposed to 60% of 4 h LC50 of azadirachtin and allicin, respectively.
authors.affiliation: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur, 273009 Uttar Pradesh, India.
authors.forename: Kumari
authors.initials: K
authors.lastname: Sunita
authors.affiliation: Human Genetics Lab., Department of Biosciences, Jamia Millia Islamia University, New Delhi 110025, India. Electronic address: ksunita705@yahoo.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Habib
authors.affiliation: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur, 273009 Uttar Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur, 273009 Uttar Pradesh, India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Singh
authors.affiliation: Human Genetics Lab., Department of Biosciences, Jamia Millia Islamia University, New Delhi 110025, India.
authors.forename: Syed Akhtar
authors.initials: SA
authors.lastname: Husain
authors.affiliation: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur, 273009 Uttar Pradesh, India. Electronic address: dksingh_gpu@yahoo.co.in.
authors.forename: D K
authors.initials: DK
authors.lastname: Singh
mesh_terms: D000818:Animals; D058487:Cercaria / Q000187:drug effects* / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D005209:Fasciola / Q000187:drug effects* / Q000201:enzymology; D008195:Lymnaea / Q000187:drug effects / Q000201:enzymology
chemical_list: D002800:Cholinesterase Inhibitors; D003576:Electron Transport Complex IV
keywords: AChE; Cercaria larva; Cercaricide; Cytochrome oxidase; Lymnaea acuminata; Nervous tissues