title: Isolation and identification of bioactive compounds from chloroform fraction of methanolic extract of Carissa opaca roots.
abstract: Carissa opaca is a shrub known for its variety of medicinal applications. This study reports isolation and identification of four chemical compounds from its roots for the first time. The methanolic extract of the roots was fractionated into various solvents with increasing polarity. Chloroform fraction was subjected to column and thin layer chromatography to ultimately yield 2H-cyclopropanaphthalene-2-one, 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one, 3-(4-methoxyphenyl)-2,6-dimethylbenzofuran and 5(1H)-azulenone, 2,4,6,7,8,8a-hexahydro-3,8-dimethyl-4-(1-methylethylidene)-,(8S-cis). They were identified by GC-MS analysis. The compounds exhibited considerable antimicrobial activities against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger with zones of inhibition ranging from 10 to 13 mm as compared to the standard drug amoxicillin with zones of inhibition 13-17 mm under the similar conditions. In conclusion, the roots of C. opaca can provide new leads for future antimicrobial drugs.
authors.affiliation: a Department of Chemistry , Forman Christian College (A Chartered University) , Lahore , Pakistan.
authors.forename: Dildar
authors.initials: D
authors.lastname: Ahmed
authors.affiliation: a Department of Chemistry , Forman Christian College (A Chartered University) , Lahore , Pakistan.
authors.forename: Khaizran
authors.initials: K
authors.lastname: Fatima
authors.affiliation: a Department of Chemistry , Forman Christian College (A Chartered University) , Lahore , Pakistan.
authors.forename: Ramsha
authors.initials: R
authors.lastname: Saeed
authors.affiliation: a Department of Chemistry , Forman Christian College (A Chartered University) , Lahore , Pakistan.
authors.forename: Rashid
authors.initials: R
authors.lastname: Masih
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029062:Apocynaceae / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D002725:Chloroform; D003374:Coumarins; D000432:Methanol; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D003374:Coumarins; D010936:Plant Extracts; D002725:Chloroform; D000432:Methanol
keywords: Carissa opaca; antibacterial; antifungal; compounds; roots