title: Single Base-Resolution Methylome of the Dizygotic Sheep.
abstract: Sheep is an important livestock in the world for meat, dairy and wool production. The third version of sheep reference genome has been recently assembled, but sheep DNA methylome has not been profiled yet. In this study, we report the comprehensive sheep methylome with 94.38% cytosine coverage at single base resolution by sequencing DNA samples from Longissimus dorsi of dizygotic Sunit sheep, which were bred in different habitats. We also compared methylomes between the twin sheep. DNA methylation status at genome-scale differentially methylated regions (DMRs), functional genomic regions and 248 DMR-containing genes were identified between the twin sheep. Gene ontology (GO) and KEGG annotations of these genes were performed to discover computationally predicted function. Lipid metabolism, sexual maturity and tumor-associated categories were observed to significantly enrich DMR-containing genes. These findings could be used to illustrate the relationship between phenotypic variations and gene methylation patterns.
authors.affiliation: Kunming University of Science and Technology, Chenggong District, Kunming, China.
authors.forename: Yangzi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.|Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, China.|Inner Mongolia Prataculture Research Center, Chinese Academy of Science, Hohhot, China.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Yunnan Agricultural University, Kunming, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.|Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.|College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Su
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.|College of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Kunming University of Science and Technology, Chenggong District, Kunming, China.|State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000818:Animals; D018899:CpG Islands; D003596:Cytosine / Q000378:metabolism; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods*; D017422:Sequence Analysis, DNA; D012756:Sheep; D014158:Transcription, Genetic
chemical_list: D003596:Cytosine