title: Preparation of Proteoglycan Mimetic Graft Copolymers.
abstract: Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains. The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans. By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities. The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end. Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
authors.affiliation: Department of Chemical and Biological Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80526-1370, USA. matthew.kipper@colostate.edu.
authors.forename: Matt J
authors.initials: MJ
authors.lastname: Kipper
authors.affiliation: Department of Chemical and Biological Engineering and School of Biomedical Engineering, Colorado State University, Fort Collins, CO, 80526-1370, USA.
authors.forename: Laura W
authors.initials: LW
authors.lastname: Place
mesh_terms: D006025:Glycosaminoglycans / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D058105:Polymerization; D011509:Proteoglycans / Q000138:chemical synthesis*
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D006820:Hyaluronic Acid
keywords: Aggrecan; Chondroitin sulfate; Glycosaminoglycans; Graft copolymers; Heparin; Hyaluronan; Proteoglycans; Versican