title: MOLECULAR CHARACTERIZATION OF FLAB FOR LEPTOSPIRA IDENTIFICATION.
abstract: PCR amplification of the nearly full-length of virulence flagellin gene (flaB) was employed for rapid identification of Leptospira spp and of Leptospira-specific 16S rDNA (rrs) for differentiation from other bacteria. This approach distinguished pathogenic from non-pathogenic Leptospira strains, and the generation of restriction fragment length polymorphism profiles using a combination of restriction endonucleases allowed identification of pathogenic Leptospira species. PCR-based identification of Leptospira flaB provides an accurate and rapid tool for identification of leptospires and can be used as a means for rapidly identifying animal reservoirs responsible for leptospirosis outbreaks. Furthermore, these techniques could be applied to clinical diagnosis without the need for leptospiral isolation.
authors.forename: Worawidh
authors.initials: W
authors.lastname: Wajjwalkul
authors.forename: Manakorn
authors.initials: M
authors.lastname: Sukmak
authors.forename: Patamaporn
authors.initials: P
authors.lastname: Amavisit
authors.forename: Thavajchai
authors.initials: T
authors.lastname: Sukpuaram
authors.forename: Anchalee
authors.initials: A
authors.lastname: La-ard
mesh_terms: D000818:Animals; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D005408:Flagellin / Q000235:genetics*; D006801:Humans; D007919:Leptospira / Q000235:genetics*; D007922:Leptospirosis / Q000175:diagnosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D005408:Flagellin; C416532:flaB flagellin