title: Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis.
abstract: BACKGROUND
  Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment.
  
  
  METHODS
  Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia).
  
  
  RESULTS
  DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively.
  
  
  CONCLUSIONS
  The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance.
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Carolina Cella
authors.initials: CC
authors.lastname: Conter
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Herintha Coeto
authors.initials: HC
authors.lastname: Neitzke-Abreu
authors.affiliation: Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Raissa Bocchi
authors.initials: RB
authors.lastname: Pedroso
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Maria Valdrinez Campana
authors.initials: MV
authors.lastname: Lonardoni
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Thaís Gomes Verzignassi
authors.initials: TG
authors.lastname: Silveira
authors.affiliation: Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
authors.forename: Sandra Mara Alessi
authors.initials: SM
authors.lastname: Aristides
mesh_terms: D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D006801:Humans; D007891:Leishmania / Q000235:genetics*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D007963:Leukocytes, Mononuclear / Q000469:parasitology*; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D016054:DNA, Protozoan