title: N-carboxyethyl chitosan fibers prepared as potential use in tissue engineering.
abstract: To improve the hydrophilicity of chitosan fiber, N-carboxyethyl chitosan fiber was prepared through Michael addition between chitosan fiber with acrylic acid. The structure was studied by (1)H NMR. The degree of N-substitution, measured via (1)H NMR, was easily varied from 0.10 to 0.51 by varying the molar ratio of acrylic acid to chitosan. Series of properties of N-carboxyethyl chitosan fiber including mechanical property, crystallinity, thermal property and in vitro degradation were investigated by Instron machine, X-ray diffraction and differential scanning calorimetry and thermogravimetric analysis, respectively. The results showed that, introducing the carboxyethyl group into the backbone chain of chitosan fiber destroyed the intra/intermolecular hydrogen bonding, leading to loss of the intra/intermolecular hydrogen bonding and improvement of hydrophilicity. Indirect cytotoxicity assessment of carboxyethyl chitosan fibers was investigated using a L929 cell line. And the obtained results clearly suggested that N-carboxyethyl chitosan fiber was nontoxic to L929 cells. The N-carboxyethyl chitosan fibers are potential as tissue engineering scaffolds.
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Shuoshuo
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Dong
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Shaojin
authors.initials: S
authors.lastname: Gu
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China. Electronic address: zyssyz@126.com.
authors.forename: Yingshan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430073, People's Republic of China; Key Laboratory of Green Processing and Functional Textiles of New Textile Materials, Ministry of Education, Wuhan Textile University, Wuhan 430073, People's Republic of China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Xu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D045744:Cell Line, Tumor; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D055595:Mechanical Phenomena; D066244:Proton Magnetic Resonance Spectroscopy; D013816:Thermodynamics; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan
keywords: Carboxyethylation; Chitosan; Fiber; Hydrophilicity; Tissue engineering