title: Genotyping of Enterocytozoon bieneusi in Farmed Blue Foxes (Alopex lagopus) and Raccoon Dogs (Nyctereutes procyonoides) in China.
abstract: Enterocytozoon bieneusi is the most common species of microsporidia found both in humans and animals. Farmed animals, particularly closely associated to humans, may play an important role of zoonotic reservoir in transmitting this disease to humans. The fur industry is a major economic component in some parts of China. To understand the prevalence, genotype variety and zoonotic risk of E. bieneusi in farmed foxes and raccoon dogs, two species of fur animals, fecal specimens of 110 blue foxes and 49 raccoon dogs from Heilongjiang and Jilin provinces in China were examined by internal transcribed spacer (ITS)-based PCR. E. bieneusi was detected in 16.4% (18/110) blue foxes and 4.1% (2/49) raccoon dogs. Altogether, four genotypes of E. bieneusi were identified, including two known genotypes D (n = 13) and EbpC (n = 5), and two novel genotypes named as CHN-F1 (n = 1) in a fox and CHN-R1 (n = 1) in a raccoon dog. Phylogenetic analysis revealed that all the four genotypes were the members of zoonotic group 1. Genotypes D and EbpC were found in humans previously. The findings of zoonotic genotypes of E. bieneusi in the foxes and raccoon dogs suggest these animals infected with E. bieneusi may pose a threat to human health.
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Weizhe
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Aiqin
authors.initials: A
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Fengkun
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Harbin Medical University China; Harbin Medical University, Heilongjiang Provincial Key Laboratory for Infection and Immunity; Heilongjiang Key Laboratory for Pathogen Biology, Harbin, Heilongjiang 150081, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ling
mesh_terms: D000818:Animals; D002681:China; D021901:DNA, Intergenic / Q000235:genetics; D021862:Enterocytozoon / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D005589:Foxes / Q000382:microbiology*; D060005:Genotyping Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D046031:Raccoon Dogs / Q000382:microbiology*
chemical_list: D021901:DNA, Intergenic