title: The respiratory DC/macrophage network at steady-state and upon influenza infection in the swine biomedical model.
abstract: Human and mouse respiratory tracts show anatomical and physiological differences, which will benefit from alternative experimental models for studying many respiratory diseases. Pig has been recognized as a valuable biomedical model, in particular for lung transplantation or pathologies such as cystic fibrosis and influenza infection. However, there is a lack of knowledge about the porcine respiratory immune system. Here we segregated and studied six populations of pig lung dendritic cells (DCs)/macrophages (Mθs) as follows: conventional DCs (cDC) 1 and cDC2, inflammatory monocyte-derived DCs (moDCs), monocyte-derived Mθs, and interstitial and alveolar Mθs. The three DC subsets present migratory and naive T-cell stimulation capacities. As observed in human and mice, porcine cDC1 and cDC2 were able to induce T-helper (Th)1 and Th2 responses, respectively. Interestingly, porcine moDCs increased in the lung upon influenza infection, as observed in the mouse model. Pig cDC2 shared some characteristics observed in human but not in mice, such as the expression of FCɛRIα and Langerin, and an intra-epithelial localization. This work, by unraveling the extended similarities of the porcine and human lung DC/Mθ networks, highlights the relevance of pig, both as an exploratory model of DC/Mθ functions and as a model for human inflammatory lung pathologies.
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: P
authors.initials: P
authors.lastname: Maisonnasse
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: E
authors.initials: E
authors.lastname: Bouguyon
authors.affiliation: INRA, UMR Génétique Animale et Biologie Intégrative (GABI), Equipe Génétique Immunité Santé, Jouy-en-Josas, France.|Laboratoire de Radiobiologie et Etude du genome, CEA, Direction des Sciences du Vivant, Institut de Radiobiologie Cellulaire et Moléculaire, Jouy-en-Josas, France.
authors.forename: G
authors.initials: G
authors.lastname: Piton
authors.affiliation: Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Urien
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Deloizy
authors.affiliation: I2BC, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: M
authors.initials: M
authors.lastname: Bourge
authors.affiliation: INRA, UMR Génétique Animale et Biologie Intégrative (GABI), Equipe Génétique Immunité Santé, Jouy-en-Josas, France.|Laboratoire de Radiobiologie et Etude du genome, CEA, Direction des Sciences du Vivant, Institut de Radiobiologie Cellulaire et Moléculaire, Jouy-en-Josas, France.
authors.forename: J-J
authors.initials: JJ
authors.lastname: Leplat
authors.affiliation: Anses, Laboratoire de Ploufragan/Plouzané, Unité Virologie Immunologie Porcines, BP53, Ploufragan, France.|Université Européenne de Bretagne, Rennes, France.
authors.forename: G
authors.initials: G
authors.lastname: Simon
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Chevalier
authors.affiliation: INRA, UMR Génétique Animale et Biologie Intégrative (GABI), Equipe Génétique Immunité Santé, Jouy-en-Josas, France.|Laboratoire de Radiobiologie et Etude du genome, CEA, Direction des Sciences du Vivant, Institut de Radiobiologie Cellulaire et Moléculaire, Jouy-en-Josas, France.
authors.forename: S
authors.initials: S
authors.lastname: Vincent-Naulleau
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: E
authors.initials: E
authors.lastname: Crisci
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.|The Pirbright Institute, Surrey, UK.
authors.forename: M
authors.initials: M
authors.lastname: Montoya
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: I
authors.initials: I
authors.lastname: Schwartz-Cornil
authors.affiliation: Virologie et Immunologie Moléculaires UR892, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: N
authors.initials: N
authors.lastname: Bertho
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D004195:Disease Models, Animal; D006801:Humans; D007251:Influenza, Human / Q000276:immunology*; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000821:virology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000821:virology; D037241:Mannose-Binding Lectins / Q000378:metabolism; D051379:Mice; D009975:Orthomyxoviridae / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D017455:Receptors, IgE / Q000378:metabolism; D012137:Respiratory System / Q000276:immunology*; D013552:Swine; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D015703:Antigens, CD; C121916:CD207 protein, human; C475664:FCER1A protein, human; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D017455:Receptors, IgE