title: Development and Characterization of Monoclonal Antibodies Specific for Mouse and Human Fcγ Receptors.
abstract: FcγRs are key regulators of the immune response, capable of binding to the Fc portion of IgG Abs and manipulating the behavior of numerous cell types. Through a variety of receptors, isoforms, and cellular expression patterns, they are able to fine-tune and direct appropriate responses. Furthermore, they are key determinants of mAb immunotherapy, with mAb isotype and FcγR interaction governing therapeutic efficacy. Critical to understanding the biology of this complex family of receptors are reagents that are robust and highly specific for each receptor. In this study, we describe the development and characterization of mAb panels specific for both mouse and human FcγR for use in flow cytometry, immunofluorescence, and immunocytochemistry. We highlight key differences in expression between the two species and also patterns of expression that will likely impact on immunotherapeutic efficacy and translation of therapeutic agents from mouse to clinic.
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Alison L
authors.initials: AL
authors.lastname: Tutt
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Sonya
authors.initials: S
authors.lastname: James
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Stéphanie A
authors.initials: SA
authors.lastname: Laversin
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Thomas R W
authors.initials: TR
authors.lastname: Tipton
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Margaret
authors.initials: M
authors.lastname: Ashton-Key
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Ruth R
authors.initials: RR
authors.lastname: French
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Khiyam
authors.initials: K
authors.lastname: Hussain
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Vaughan
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Lang
authors.initials: L
authors.lastname: Dou
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Alexander
authors.initials: A
authors.lastname: Earley
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Lekh N
authors.initials: LN
authors.lastname: Dahal
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Chen
authors.initials: C
authors.lastname: Lu
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Melanie
authors.initials: M
authors.lastname: Dunscombe
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: H T Claude
authors.initials: HT
authors.lastname: Chan
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Penfold
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Jinny H
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Potter
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: C Ian
authors.initials: CI
authors.lastname: Mockridge
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Ali
authors.initials: A
authors.lastname: Roghanian
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Oldham
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Kerry L
authors.initials: KL
authors.lastname: Cox
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Sean H
authors.initials: SH
authors.lastname: Lim
authors.affiliation: BioInvent International, 22362 Lund, Sweden.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Teige
authors.affiliation: BioInvent International, 22362 Lund, Sweden.
authors.forename: Bjorn
authors.initials: B
authors.lastname: Frendéus
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Glennie
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Beers
authors.affiliation: Antibody and Vaccine Group, Cancer Sciences Unit, University of Southampton Faculty of Medicine, Southampton General Hospital, Southampton, Hampshire SO16 6YD, United Kingdom; and msc@soton.ac.uk.
authors.forename: Mark S
authors.identifier: http://orcid.org/0000-0003-2077-089X
authors.initials: MS
authors.lastname: Cragg
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014066:Palatine Tonsil / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000276:immunology*; D013154:Spleen / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D020033:Protein Isoforms; D017452:Receptors, IgG