title: Transcriptome Analysis of Two Vicia sativa Subspecies: Mining Molecular Markers to Enhance Genomic Resources for Vetch Improvement.
abstract: The vetch (Vicia sativa) is one of the most important annual forage legumes globally due to its multiple uses and high nutritional content. Despite these agronomical benefits, many drawbacks, including cyano-alanine toxin, has reduced the agronomic value of vetch varieties. Here, we used 454 technology to sequence the two V. sativa subspecies (ssp. sativa and ssp. nigra) to enrich functional information and genetic marker resources for the vetch research community. A total of 86,532 and 47,103 reads produced 35,202 and 18,808 unigenes with average lengths of 735 and 601 bp for V. sativa sativa and V. sativa nigra, respectively. Gene Ontology annotations and the cluster of orthologous gene classes were used to annotate the function of the Vicia transcriptomes. The Vicia transcriptome sequences were then mined for simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. About 13% and 3% of the Vicia unigenes contained the putative SSR and SNP sequences, respectively. Among those SSRs, 100 were chosen for the validation and the polymorphism test using the Vicia germplasm set. Thus, our approach takes advantage of the utility of transcriptomic data to expedite a vetch breeding program.
authors.affiliation: Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 340-702, Korea. tk227gm@gmail.com.
authors.forename: Tae-Sung
authors.initials: TS
authors.lastname: Kim
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. raveendars@gmail.com.
authors.forename: Sebastin
authors.initials: S
authors.lastname: Raveendar
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. sureshplant@gmail.com.|Department of Botany, Directorate of Distance Education, Madurai Kamaraj University, Madurai 625 021, India. sureshplant@gmail.com.
authors.forename: Sundan
authors.initials: S
authors.lastname: Suresh
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. gkntl1@korea.kr.
authors.forename: Gi-An
authors.initials: GA
authors.lastname: Lee
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. jrmail@korea.kr.
authors.forename: Jung-Ro
authors.initials: JR
authors.lastname: Lee
authors.affiliation: Department of Biological and Environmental Science, Dongguk University, Seoul 100-175, Korea. jrmail@korea.kr.
authors.forename: Joon-Hyeong
authors.initials: JH
authors.lastname: Cho
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. lsy007@korea.kr.
authors.forename: Sok-Young
authors.initials: SY
authors.lastname: Lee
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. khma@korea.kr.
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Ma
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. gtcho@korea.kr.
authors.forename: Gyu-Taek
authors.initials: GT
authors.lastname: Cho
authors.affiliation: National Agrobiodiversity Centre, National Academy of Agricultural Science, Rural Development Administration, Jeonju 560-500, Korea. jwchung73@korea.kr.
authors.forename: Jong-Wook
authors.initials: JW
authors.lastname: Chung
keywords: 454 pyrosequencing; SNPs; SSRs; the vetch (Vicia sativa); transcriptome