title: A study of vertebra number in pigs confirms the association of vertnin and reveals additional QTL.
abstract: BACKGROUND
  Formation of the vertebral column is a critical developmental stage in mammals. The strict control of this process has resulted in little variation in number of vertebrae across mammalian species and no variation within most mammalian species. The pig is quite unique as considerable variation exists in number of thoracic vertebrae as well as number of lumbar vertebrae. At least two genes have been identified that affect number of vertebrae in pigs yet considerable genetic variation still exists. Therefore, a genome-wide association (GWA) analysis was conducted to identify additional genomic regions that affect this trait.
  
  
  RESULTS
  A total of 1883 animals were phenotyped for the number of ribs and thoracolumbar vertebrae as well as successfully genotyped with the Illumina Porcine SNP60 BeadChip. After data editing, 41,148 SNP markers were included in the GWA analysis. These animals were also phenotyped for kyphosis. Fifty-three 1 Mb windows each explained at least 1.0 % of the genomic variation for vertebrae counts while 16 regions were significant for kyphosis. Vertnin genotype significantly affected vertebral counts as well. The region with the largest effect for number of lumbar vertebrae and thoracolumbar vertebrae were located over the Hox B gene cluster and the largest association for thoracic vertebrae number was over the Hox A gene cluster. Genetic markers in significant regions accounted for approximately 50% of the genomic variation. Less genomic variation for kyphosis was described by QTL regions and no region was associated with kyphosis and vertebra counts.
  
  
  CONCLUSIONS
  The importance of the Hox gene families in vertebral development was highlighted as significant associations were detected over the A, B and C families. Further evaluation of these regions and characterization of variants within these genes will expand our knowledge on vertebral development using natural genetic variants segregating in commercial swine.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service,, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA. Gary.Rohrer@ars.usda.gov.
authors.forename: Gary A
authors.initials: GA
authors.lastname: Rohrer
authors.affiliation: United States Department of Agriculture, Agricultural Research Service,, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA. Dan.Nonneman@ars.usda.gov.
authors.forename: Dan J
authors.initials: DJ
authors.lastname: Nonneman
authors.affiliation: United States Department of Agriculture, Agricultural Research Service,, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA. Wiedmann@windstream.net.
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Wiedmann
authors.affiliation: United States Department of Agriculture, Agricultural Research Service,, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA. james.f.schneider@gmail.com.
authors.forename: James F
authors.initials: JF
authors.lastname: Schneider
mesh_terms: D000818:Animals; D033481:Chromosomes, Mammalian / Q000235:genetics; D055106:Genome-Wide Association Study; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics*; D013131:Spine / Q000033:anatomy & histology*