title: HSPC280, a winged helix protein expressed in the subventricular zone of the developing ganglionic eminences, inhibits neuronal differentiation.
abstract: Winged helix proteins have critical roles in a variety of developmental processes. During a screening for genes expressed in the developing forebrain, we identified HSPC280, a non-typical winged helix protein, which shares similarity with a protein-protein interaction domain found in the proteins of the actin-binding Rho-activating protein family. In this work, we analyzed HSPC280 expression during mouse development as well as during neuronal differentiation of mouse Neuro2a cells. HSPC280 expression is tightly regulated; during mouse development, it was detected predominantly in the ganglionic eminences of the ventral telencephalon, from their appearance at E11.5 to P0, with the highest levels between E13.5 and E15.5, a period that correlates with the peak of neurogenesis in these structures. Comparative expression analysis of HSPC280 with Dlx2, cyclinD2 and Lhx6 revealed that, within the ganglionic eminences, HSPC280 was restricted in the proliferating cell population of the subventricular zone, in a pattern similar to that of cyclinD2. Finally, we showed that HSPC280 is a nuclear protein which, when overexpressed in Neuro2a cells, it inhibited neuronal differentiation in vitro, suggesting its involvement in the mechanisms controlling neural progenitor cells proliferation.
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece.
authors.forename: Electra
authors.initials: E
authors.lastname: Stylianopoulou
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece.
authors.forename: Georgios
authors.initials: G
authors.lastname: Kalamakis
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece.
authors.forename: Margarita
authors.initials: M
authors.lastname: Pitsiani
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Fysekis
authors.affiliation: Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Petros
authors.initials: P
authors.lastname: Ypsilantis
authors.affiliation: Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Simopoulos
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Skavdis
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Building 10, University Campus, Dragana, 68100, Alexandroupolis, Greece. mgrigor@mbg.duth.gr.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Grigoriou
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005260:Female; D051858:Forkhead Transcription Factors / Q000096:biosynthesis* / Q000378:metabolism*; D005724:Ganglia / Q000166:cytology* / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D020547:Lateral Ventricles / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C000611722:HSPC280 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins
keywords: Abra; Proliferation; Subventricular zone; Ventral forebrain; Winged helix domain