title: An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer.
abstract: A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix assembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and differential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and morphological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid carboxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical performance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ngmL(-1) (r=0.995, p«0.01), with a very low limit of detection (0.006 ngmL(-1)). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with excellent affinity to cTnT binding (KD=7.3 10(-13) molL(-1)). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Bárbara V M
authors.initials: BV
authors.lastname: Silva
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil.
authors.forename: Blanca A G
authors.initials: BA
authors.lastname: Rodríguez
authors.affiliation: BioMark/ISEP, School of Engineering, Polytechnic Institute of Porto, Porto, Portugal.
authors.forename: Goreti F
authors.initials: GF
authors.lastname: Sales
authors.affiliation: Chemistry Institute, State University of São Paulo (UNESP), Araraquara, Brazil.
authors.forename: Maria Del Pilar T
authors.initials: Mdel P
authors.lastname: Sotomayor
authors.affiliation: Biomedical Engineering Laboratory, Federal University of Pernambuco, Recife, Brazil. Electronic address: rosa.dutra@ufpe.br.
authors.forename: Rosa F
authors.initials: RF
authors.lastname: Dutra
mesh_terms: D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004566:Electrodes; D004595:Electroplating / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D054802:Molecular Imprinting / Q000379:methods*; D010781:Photography / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020107:Troponin T / Q000097:blood*
chemical_list: D020107:Troponin T; D006108:Graphite
keywords: Acid carboxylic-3-pyrrole; Cardiac troponin T; Graphene; Molecularly imprinted polymer; Point-of-care testing; Pyrrole