title: Studying Protein-Protein Interactions in Budding Yeast Using Co-immunoprecipitation.
abstract: Understanding protein-protein interactions and the architecture of protein complexes in which they work is essential to identify their biological role. Protein co-immunoprecipitation (co-IP) is an invaluable technique used in biochemistry allowing the identification of protein interactors. Here, we describe in detail an immunoaffinity purification protocol as a one-step or two-step immunoprecipitation from budding yeast Saccharomyces cerevisiae cells to subsequently detect interactions between proteins involved in the same biological process.
authors.affiliation: Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, Albert Einstein 22, Santander, 39011, Spain.|Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, Santander, 39011, Spain.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Foltman
authors.affiliation: Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Cardenal Herrera Oria s/n, Santander, 39011, Spain. a.sanchezdiaz@unican.es.|Instituto de Biomedicina y Biotecnología de Cantabria, Consejo Superior de Investigaciones Científicas, Universidad de Cantabria, Albert Einstein 22, Santander, 39011, Spain. a.sanchezdiaz@unican.es.
authors.forename: Alberto
authors.initials: A
authors.lastname: Sanchez-Diaz
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D047468:Immunoprecipitation / Q000379:methods*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D004718:Saccharomycetales / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins
keywords: Antibody; Cell extract; Co-immunoprecipitation; Freezer/Mill grinder; Immunoaffinity purification; Magnetic bead s; Protein complexes; Protein interaction s