title: [Determination of seven phthalate metabolites in human urine by high performance liquid chromatography-tandem mass spectrometry].
abstract: A method has been developed for the analysis of seven metabolites of phthalates in human urine by high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS). The urine samples were hydrolyzed with glucuronidase followed by purification with solid-phase extraction (SPE) cartridges. Both 0. 1% formic acid in water (v/v) and 0.1% formic acid in acetonitrile were used as the mobile phases in a gradient mode. The chromatographic separation was achieved on a phenyl column. Mass detection was then conducted by electrospray ionization in negative ion mode and multiple reaction monitoring mode. The components were quantified by stable isotope-labelled (13C-) phthalate monoester internal standards. The calibration curves of the seven phthalates metabolites showed good linear relationships in the range of 0.2-200.0 µg/L (r > 0.999 76). The recoveries at three levels were from 88.8% to 108.9% with relative standard deviations no more than 17.05%. The limits of detection of the method were 13.43-80.2 ng/L. The limits of quantification were 44.77-267.37 ng/L. This method was successfully applied to the determination of metabolism of phthalates in human urine with efficiency, increased accuracy and high sensitivity.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Zhongxiu
authors.initials: Z
authors.lastname: Jin
authors.forename: Haiting
authors.initials: H
authors.lastname: Hu
authors.forename: Jie
authors.initials: J
authors.lastname: Sheng
authors.forename: Lingling
authors.initials: L
authors.lastname: Ren
authors.forename: Fangbiao
authors.initials: F
authors.lastname: Tao
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010795:Phthalic Acids / Q000378:metabolism / Q000652:urine*; D012015:Reference Standards; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D010795:Phthalic Acids; C032279:phthalic acid