title: Influence of Klotho gene polymorphisms on vascular gene expression and its relationship to cardiovascular disease.
abstract: Klotho protein has been associated with beneficial effects that contribute to the maintenance of cardiovascular health. Diverse studies suggest that alterations in the levels of this molecule may be associated with pathophysiological abnormalities that result in increased cardiovascular risk. The primary aim of this proof-of-concept study was to analyse the existence of a potential link between Klotho gene polymorphisms and the expression level of this gene in the vascular wall, and additionally with the incidence of cardiovascular disease and cardiovascular risk factors. Our results indicate that the variant G-395A, located in the promoter region, influences Klotho gene vascular expression and is associated with the incidence of diabetes. Similarly, the exonic variant KL-VS was associated with the incidence of atherosclerotic vascular disease and coronary artery disease. Moreover, vascular expression levels of Klotho were related with the incidence of diabetes mellitus and coronary artery disease. These findings, which need to be confirmed in larger studies, suggest a potential role of Klotho in the pathogenesis of vascular damage.
authors.affiliation: Research Unit, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Donate-Correa
authors.affiliation: Research Unit, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Martín-Núñez
authors.affiliation: Cardiovascular Surgery Service, Hospital Universitario de Canarias, Santa Cruz de Tenerife, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Martínez-Sanz
authors.affiliation: Clinical Biochemistry Service, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Muros-de-Fuentes
authors.affiliation: Research Unit, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mora-Fernández
authors.affiliation: Clinical Biochemistry Service, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Nayra
authors.initials: N
authors.lastname: Pérez-Delgado
authors.affiliation: Research Unit, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.|Nephrology Service, University Hospital Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Spain.
authors.forename: Juan F
authors.identifier: http://orcid.org/0000-0002-5015-7474
authors.initials: JF
authors.lastname: Navarro-González
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001024:Aortic Valve Stenosis / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D001026:Coronary Artery Bypass; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005966:Glucuronidase / Q000235:genetics*; D006349:Heart Valve Diseases / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D000090265:Klotho Proteins; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D059467:Transcriptome
chemical_list: D005966:Glucuronidase; D000090265:Klotho Proteins
keywords: Klotho; single nucleotide polymorphisms; vascular expression