title: Efficient nitro reduction and dechlorination of 2,4-dinitrochlorobenzene through the integration of bioelectrochemical system into upflow anaerobic sludge blanket: A comprehensive study.
abstract: Bioelectrochemical system (BES) coupled upflow anaerobic sludge blanket (UASB) was developed for the removal of recalcitrant pollutants but lack of a comprehensive study. Thus in this study an integrated UASB-BES system was operated continuously for 240 d to systematically investigate the feasibility of the enhanced reduction of 2,4-dinitrochlorobenzene (DNCB), with the key operation parameters, the system stability as well as the microbial biodiversity emphasized. The results indicate that high voltage supplied had a positive effect on DNCB reduction but a negative impact for the overhigh voltage (>1.6 V). The ability to resist shock loading was strengthened in the UASB-BES system in comparison with the control UASB system. High-throughput sequencing analysis suggested that the enhanced reduction of DNCB in UASB-BES could be attributed to higher diversity and the enrichment of reduction-related species, potential electroactive species and fermentative species. Both DNCB removal and dechlorination gradually increased with the increase of operation time, indicating the improved performance of the coupled UASB-BES system. The heatmap visualized only slight differences in the microbial community during long-term operation, indicating the stability of the microbial community. The observed efficient and stable performance highlights the potential for long-term operation and full-scale application of the UASB-BES coupled system particularly for highly recalcitrant pollutants removal.
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Xinbai
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China. Electronic address: shenjinyou@mail.njust.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Shen
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Lou
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Sun
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China.
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, Anhui Province, China. Electronic address: yangmu@ustc.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Jiangsu Key Laboratory for Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China. Electronic address: wanglj@mail.njust.edu.cn.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Wang
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology*; D004137:Dinitrochlorobenzene / Q000737:chemistry* / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D054879:Halogenation; D059013:Microbial Consortia; D054712:Salinity; D012722:Sewage; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
chemical_list: D004137:Dinitrochlorobenzene; D012722:Sewage; D014874:Water Pollutants, Chemical
keywords: Bioelectrochemical system (BES); Chloronitrobenzenes (ClNBs); Microbial community analysis; Reduction; Upflow anaerobic sludge blanket (UASB)