title: Simultaneous quantification of 21 water soluble vitamin circulating forms in human plasma by liquid chromatography-mass spectrometry.
abstract: This manuscript reports a validated analytical approach for the quantification of 21 water soluble vitamins and their main circulating forms in human plasma. Isotope dilution-based sample preparation consisted of protein precipitation using acidic methanol enriched with stable isotope labelled internal standards. Separation was achieved by reversed-phase liquid chromatography and detection performed by tandem mass spectrometry in positive electrospray ionization mode. Instrumental lower limits of detection and quantification reached <0.1-10nM and 0.2-25nM, respectively. Commercially available pooled human plasma was used to build matrix-matched calibration curves ranging 2-500, 5-1250, 20-5000 or 150-37500nM depending on the analyte. The overall performance of the method was considered adequate, with 2.8-20.9% and 5.2-20.0% intra and inter-day precision, respectively and averaged accuracy reaching 91-108%. Recovery experiments were also performed and reached in average 82%. This analytical approach was then applied for the quantification of circulating water soluble vitamins in human plasma single donor samples. The present report provides a sensitive and reliable approach for the quantification of water soluble vitamins and main circulating forms in human plasma. In the future, the application of this analytical approach will give more confidence to provide a comprehensive assessment of water soluble vitamins nutritional status and bioavailability studies in humans.
authors.affiliation: Vitamins & Phytonutrients Group, Analytical Sciences Department, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland. Electronic address: Karine.Meisser@rdls.nestle.com.
authors.forename: Karine
authors.initials: K
authors.lastname: Meisser Redeuil
authors.affiliation: Vitamins & Phytonutrients Group, Analytical Sciences Department, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Karin
authors.initials: K
authors.lastname: Longet
authors.affiliation: Vitamins & Phytonutrients Group, Analytical Sciences Department, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bénet
authors.affiliation: CT - R&D Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Caroline
authors.initials: C
authors.lastname: Munari
authors.affiliation: Vitamins & Phytonutrients Group, Analytical Sciences Department, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
authors.forename: Esther
authors.initials: E
authors.lastname: Campos-Giménez
mesh_terms: D001682:Biological Availability; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid; D006801:Humans; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D014815:Vitamins / Q000097:blood*; D014867:Water / Q000737:chemistry
chemical_list: D014815:Vitamins; D014867:Water
keywords: Circulating forms; Human plasma; Liquid chromatography; Tandem mass spectrometry; Water soluble vitamins