title: Galantamine attenuates amyloid-β deposition and astrocyte activation in APP/PS1 transgenic mice.
abstract: While galantamine may not provide a cure for Alzheimer's disease (AD), it decelerates the progression and provides symptomatic relief for this disorder. The aim of this study was to investigate the effect of chronic galantamine treatment on cognitive performance, Aβ deposition and astrocyte activation in the transgenic APP/PS1 mouse model of AD. Galantamine (5mg/kg, i.p.) or 0.9% saline was administrated twice daily for eight weeks in 10-month-old APP/PS1 mice. In addition a separate group of 10-month old male C57BL/6 wild type mice was included as a reference control. Compared with saline treated APP/PS1 mice, galantamine treated mice exhibited significantly improved escape latencies on Days 6 and 7 of testing (p<0.05) and significantly decreased numbers of platform crossings (p<0.01) as assessed in the Morris water maze. Galantamine reduced the total area of amyloid load within the hippocampus of transgenic APP/PS1 mice, inhibited astrocyte activation as assessed by immunohistochemistry and decreased intracellular TNF-α and IL-6 expression as determined by immunofluorescence. Galantamine failed to alter hippocampal levels of TNF-α and IL-6 as assayed by ELISA. Galantamine may be involved in modifying AD pathophysiological mechanisms by alleviating Aβ deposition and neuroinflammation. The results from this study provide new evidence for use of galantamine in the treatment of AD.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province, PR China.
authors.forename: Zhaoxuan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province, PR China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province, PR China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin, Hei Longjiang Province, PR China. Electronic address: zhangyanhyd@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects*; D004195:Disease Models, Animal; D005702:Galantamine / Q000008:administration & dosage*; D006624:Hippocampus / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D055495:Neurogenesis / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D016229:Amyloid beta-Peptides; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005702:Galantamine
keywords: Amyloid beta; Astrocyte; Galantamine; IL-6; TNF-α