title: Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.
abstract: Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.
authors.affiliation: These authors contributed equally to the work.
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Park
authors.forename: Ranawaka A P M
authors.initials: RA
authors.lastname: Perera
authors.forename: Pyoeng Gyun
authors.initials: PG
authors.lastname: Choe
authors.forename: Eric H Y
authors.initials: EH
authors.lastname: Lau
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: June Young
authors.initials: JY
authors.lastname: Chun
authors.forename: Hong Sang
authors.initials: HS
authors.lastname: Oh
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Song
authors.forename: Ji Hwan
authors.initials: JH
authors.lastname: Bang
authors.forename: Eu Suk
authors.initials: ES
authors.lastname: Kim
authors.forename: Hong Bin
authors.initials: HB
authors.lastname: Kim
authors.forename: Wan Beom
authors.initials: WB
authors.lastname: Park
authors.forename: Nam Joong
authors.initials: NJ
authors.lastname: Kim
authors.forename: Leo Lit Man
authors.initials: LL
authors.lastname: Poon
authors.forename: Malik
authors.initials: M
authors.lastname: Peiris
authors.forename: Myoung Don
authors.initials: MD
authors.lastname: Oh
mesh_terms: D000328:Adult; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002162:Camelus / Q000097:blood; D018352:Coronavirus Infections / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D065207:Middle East Respiratory Syndrome Coronavirus / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests / Q000379:methods*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral