title: Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes.
abstract: We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number of sperm bound to the PVM in fresh (T1) and frozen-thawed (T2) semen from both species was compared to the sperm quality observed in routine tests. The PVM was stretched on a circular silicone device to create a standardized area for analysis. In both treatments and for both species, the spermatozoa were able to bind to the PVM, indicating that PVM may be used for a sperm binding assay in ocelot and oncilla. The S-PVM assay did not differ in fresh and frozen-thawed ocelot sperm (p>0.05). However, fewer oncilla sperm (p<0.05) were bound to the PVM in T2, indicating that the proposed test may be able to detect injuries that compromise sperm binding abilities. The device maintained the PVM stretched during the processing and defined the evaluation area.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Gediendson Ribeiro
authors.initials: GR
authors.lastname: de Araujo
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Tarcizio Antônio Rego
authors.initials: TA
authors.lastname: de Paula
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil. Electronic address: tarcizio@ufv.br.
authors.forename: Thyara de
authors.initials: Td
authors.lastname: Deco-Souza
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Rafael de Morais
authors.initials: Rde M
authors.lastname: Garay
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Letícia Bergo
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Antônio Carlos
authors.initials: AC
authors.lastname: Csermak-Júnior
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Leanes Cruz
authors.initials: LC
authors.lastname: da Silva
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa (UFV), Av. P.H. Rolfs s/n, Centro, Viçosa MG 36570-000, Brazil.
authors.forename: Saullo Vinícius Pereira
authors.initials: SV
authors.lastname: Alves
mesh_terms: D000818:Animals; D002645:Chickens; D015925:Cryopreservation / Q000662:veterinary; D045989:Felidae / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D014817:Vitelline Membrane / Q000502:physiology*
keywords: Leopardus pardalis; Leopardus tigrinus; Semen