title: Effect of α7n-Acetylcholine Receptor Activation and Antibodies to TNF-α on Mortality of Mice and Concentration of Proinflammatory Cytokines During Early Stage of Sepsis.
abstract: Experiments on random-bred albino mice showed that activation α7n-acetylcholine receptors with anabasine (0.5 LD50) and the use of antibodies to TNF-α (1 mg/kg) 2 h before sepsis modeling significantly reduces mortality of mice from experimental sepsis (intraperitoneal injection of E. coli) due to a decrease in the blood concentration of proinflammatory cytokines TNF-α, IL-1β, and IL-6. After combined administration of anti-TNF-α antibodies and anabasine, an additive effect was observed.
authors.affiliation: Saratov Branch of Samara Medical Institute REAVIZ, Saratov, Russia. pfzabrodsky@gmail.com.
authors.forename: P F
authors.initials: PF
authors.lastname: Zabrodskii
authors.affiliation: Saratov Branch of Samara Medical Institute REAVIZ, Saratov, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Gromov
authors.affiliation: Saratov Branch of Samara Medical Institute REAVIZ, Saratov, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Maslyakov
mesh_terms: D000691:Anabasine / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D016207:Cytokines / Q000097:blood*; D018450:Disease Progression; D005260:Female; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D051379:Mice; D018805:Sepsis / Q000097:blood / Q000401:mortality* / Q000473:pathology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000276:immunology*; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000819:agonists*
chemical_list: D000906:Antibodies; D016207:Cytokines; D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; D064569:alpha7 Nicotinic Acetylcholine Receptor; D000691:Anabasine
keywords: antibodies to TNF-α; cholinergic anti-inflammatory pathway; proinflammatory cytokines; sepsis; α7n-acetylcholine receptors