title: Complete genome sequences and analysis of the Fusobacterium nucleatum subspecies animalis 7-1 bacteriophage ɸFunu1 and ɸFunu2.
abstract: Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demonstrating a more virulent phenotype than others; however, as yet the genetic basis for these differences is not understood. Bacteriophage are known to contribute to the virulence phenotype of several bacterial species. In this work, we set out to characterize the bacteriophage associated with F. nucleatum subsp. animalis strain 7-1, a highly invasive isolate from the human gastrointestinal tract. As well, we used computational approaches to predict and compare bacteriophage signatures across available sequenced F. nucleatum genomes.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Kyla
authors.initials: K
authors.lastname: Cochrane
authors.affiliation: Broad Institute of Harvard and M.I.T., 415 Main Street, Cambridge, Massachusetts 02142, USA.
authors.forename: Abigail Manson
authors.initials: AM
authors.lastname: McGuire
authors.affiliation: Broad Institute of Harvard and M.I.T., 415 Main Street, Cambridge, Massachusetts 02142, USA.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Priest
authors.affiliation: Broad Institute of Harvard and M.I.T., 415 Main Street, Cambridge, Massachusetts 02142, USA.
authors.forename: Amr
authors.initials: A
authors.lastname: Abouelleil
authors.affiliation: Broad Institute of Harvard and M.I.T., 415 Main Street, Cambridge, Massachusetts 02142, USA.
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: Cerqueira
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Reggie
authors.initials: R
authors.lastname: Lo
authors.affiliation: Broad Institute of Harvard and M.I.T., 415 Main Street, Cambridge, Massachusetts 02142, USA.
authors.forename: Ashlee M
authors.initials: AM
authors.lastname: Earl
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Emma
authors.initials: E
authors.lastname: Allen-Vercoe
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004279:DNA, Viral; D016967:Fusobacterium nucleatum / Q000821:virology*; D016679:Genome, Viral; D023281:Genomics / Q000379:methods; D006801:Humans; D058977:Molecular Sequence Annotation; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral