title: Draft Genome Sequences of Two Species of "Difficult-to-Identify" Human-Pathogenic Corynebacteria: Implications for Better Identification Tests.
abstract: Non-diphtheriae Corynebacterium species have been increasingly recognized as the causative agents of infections in humans. Differential identification of these bacteria in the clinical microbiology laboratory by the most commonly used biochemical tests is challenging, and normally requires additional molecular methods. Herein, we present the annotated draft genome sequences of two isolates of "difficult-to-identify" human-pathogenic corynebacterial species: C. xerosis and C. minutissimum. The genome sequences of ca. 2.7 Mbp, with a mean number of 2,580 protein encoding genes, were also compared with the publicly available genome sequences of strains of C. amycolatum and C. striatum. These results will aid the exploration of novel biochemical reactions to improve existing identification tests as well as the development of more accurate molecular identification methods through detection of species-specific target genes for isolate's identification or drug susceptibility profiling.
authors.affiliation: 1. Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
authors.forename: Luis G C
authors.initials: LG
authors.lastname: Pacheco
authors.affiliation: 2. Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Mattos-Guaraldi
authors.affiliation: 1. Institute of Health Sciences, Federal University of Bahia (UFBA), Salvador, BA, Brazil.
authors.forename: Carolina S
authors.initials: CS
authors.lastname: Santos
authors.affiliation: 3. Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
authors.forename: Adonney A O
authors.initials: AA
authors.lastname: Veras
authors.affiliation: 3. Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil. ; 4. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Luis C
authors.initials: LC
authors.lastname: Guimarães
authors.affiliation: 4. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Vinícius
authors.initials: V
authors.lastname: Abreu
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Felipe L
authors.initials: FL
authors.lastname: Pereira
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Siomar C
authors.initials: SC
authors.lastname: Soares
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Alex F
authors.initials: AF
authors.lastname: Carvalho
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Leal
authors.affiliation: 5. National Reference Laboratory for Aquatic Animal Diseases, Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Henrique C P
authors.initials: HC
authors.lastname: Figueiredo
authors.affiliation: 2. Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil. ; 6. National Institute for Quality Control in Health - Oswaldo Cruz Foundation (INCQS - Fiocruz), Rio de Janeiro, RJ, Brazil.
authors.forename: Juliana N
authors.initials: JN
authors.lastname: Ramos
authors.affiliation: 6. National Institute for Quality Control in Health - Oswaldo Cruz Foundation (INCQS - Fiocruz), Rio de Janeiro, RJ, Brazil.
authors.forename: Veronica V
authors.initials: VV
authors.lastname: Vieira
authors.affiliation: 7. Unité de Prévention et Thérapie Moléculaires des Maladies Humaines, Institut Pasteur, Paris, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Farfour
authors.affiliation: 7. Unité de Prévention et Thérapie Moléculaires des Maladies Humaines, Institut Pasteur, Paris, France.
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
authors.affiliation: 2. Faculty of Medical Sciences, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil.
authors.forename: Raphael
authors.initials: R
authors.lastname: Hirata
authors.affiliation: 4. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: 3. Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.affiliation: 3. Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, PA, Brazil.
authors.forename: Rommel T J
authors.initials: RT
authors.lastname: Ramos
keywords: Biochemical tests; Corynebacterium spp.; Emerging pathogens; Molecular identification