title: Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis.
abstract: Osteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was largely unknown. The purpose of this study was to evaluate the therapeutic effect of intra-articular injection of CDCA in a rabbit OA model. OA was induced in experimental rabbits by anterior cruciate ligament transection (ACLT) and then rabbits were intra-articularly injected with CDCA (10 mg/kg or 50 mg/kg) once per week for 5 weeks. The results showed that CDCA significantly decreased cartilage degradation on the surface of femoral condyles, reducing the pathological changes of articular cartilage and synovial membrane by macroscopic and histological analysis. CDCA also significantly decreased bone destruction and erosion of joint evaluated by micro-CT. Furthermore, CDCA could markedly reduce the release of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in synovial fluid. These observations highlight CDCA might be a potential therapeutic agent for OA.
authors.affiliation: Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
authors.forename: Zhao-wei
authors.initials: ZW
authors.lastname: Yan
authors.affiliation: Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China.
authors.forename: Chen-hao
authors.initials: CH
authors.lastname: Qin
authors.affiliation: Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China.
authors.forename: Chun-yang
authors.initials: CY
authors.lastname: Zhao
authors.affiliation: Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China.
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Miao
authors.affiliation: Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China.
authors.forename: Chun-yan
authors.initials: CY
authors.lastname: He
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000601:surgery; D002358:Cartilage, Articular / Q000473:pathology; D002635:Chenodeoxycholic Acid / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005269:Femur / Q000473:pathology; D007270:Injections, Intra-Articular; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy*; D011458:Prostaglandins E / Q000378:metabolism; D011817:Rabbits; D013583:Synovial Membrane / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: D053583:Interleukin-1beta; D011458:Prostaglandins E; D002635:Chenodeoxycholic Acid; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1