title: Orally administered glycidol and its fatty acid esters as well as 3-MCPD fatty acid esters are metabolized to 3-MCPD in the F344 rat.
abstract: IARC has classified glycidol and 3-monochloropropane-1,2-diol (3-MCPD) as group 2A and 2B, respectively. Their esters are generated in foodstuffs during processing and there are concerns that they may be hydrolyzed to the carcinogenic forms in vivo. Thus, we conducted two studies. In the first, we administered glycidol and 3-MCPD and associated esters (glycidol oleate: GO, glycidol linoleate: GL, 3-MCPD dipalmitate: CDP, 3-MCPD monopalmitate: CMP, 3-MCPD dioleate: CDO) to male F344 rats by single oral gavage. After 30 min, 3-MCPD was detected in serum from all groups. Glycidol was detected in serum from the rats given glycidol or GL and CDP and CDO in serum from rats given these compounds. In the second, we examined if metabolism occurs on simple reaction with rat intestinal contents (gastric, duodenal and cecal contents) from male F344 gpt delta rats. Newly produced 3-MCPD was detected in all gut contents incubated with the three 3-MCPD fatty acid esters and in gastric and duodenal contents incubated with glycidol and in duodenal and cecal contents incubated with GO. Although our observation was performed at 1 time point, the results showed that not only 3-MCPD esters but also glycidol and glycidol esters are metabolized into 3-MCPD in the rat.
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan; Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1, Yanagido, Gifu, 501-1193, Japan.
authors.forename: Saeko
authors.initials: S
authors.lastname: Onami
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Young-Man
authors.initials: YM
authors.lastname: Cho
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Toyoda
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Akagi
authors.affiliation: Division of Pharmaceutical and Life Sciences, Shimadzu Techno-Research Inc., 1, Nishinokyo-Shimoaicho, Nakagyo-ku, Kyoto, 604-8436, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fujiwara
authors.affiliation: Division of Pharmaceutical and Life Sciences, Shimadzu Techno-Research Inc., 1, Nishinokyo-Shimoaicho, Nakagyo-ku, Kyoto, 604-8436, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Ochiai
authors.affiliation: Division of Pharmaceutical and Life Sciences, Shimadzu Techno-Research Inc., 1, Nishinokyo-Shimoaicho, Nakagyo-ku, Kyoto, 604-8436, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Tsujino
authors.affiliation: Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1, Yanagido, Gifu, 501-1193, Japan; Biological Safety Research Center, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Nishikawa
authors.affiliation: Division of Pathology, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan. Electronic address: ogawa93@nihs.go.jp.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001711:Biotransformation; D002432:Cecum / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D004852:Epoxy Compounds / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D004952:Esters / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D005227:Fatty Acids / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D005753:Gastric Mucosa / Q000378:metabolism; D006868:Hydrolysis; D008297:Male; D020005:Propanols / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism* / Q000633:toxicity; D011916:Rats, Inbred F344; D000517:alpha-Chlorohydrin / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism* / Q000633:toxicity
chemical_list: D004852:Epoxy Compounds; D004952:Esters; D005227:Fatty Acids; D020005:Propanols; D000517:alpha-Chlorohydrin; C004312:glycidol
keywords: 3-MCPD; Fatty acid esters; Glycidol; Linoleate; Metabolism; Palmitate