title: [Effects of Different Dose of Cyclophosphamide on Peripheral Blood Cells of Normal ICR Mice].
abstract: OBJECTIVE
  To investigate effective doses of cyclophosphamide (CTX) for establishment of leukopenia model in ICR mouse.
  
  
  METHODS
  Normal ICR mice (n = 96) were divided into CTX(80), CTX(100), CTX(120), CTX(150) and CTX(300) groups, of which mice received CTX intraperitoneally at a dose of 80, 100, 120, or 150 mg/kg once a day for 3 consecutive days, or 300 mg/kg with single injection. The peripheral blood cell counts were detected at various times before and after CTX administration.
  
  
  RESULTS
  The peripheral white blood cell nadirs in the mice injected with CTX appeared on day 4 after the first dose of CTX. The peripheral white blood cell nadir in group CTX(100) was 26.7% of the value measured in mice before CTX administrated, and that of group CTX(80) was 35.0%. Higher doses of CTX, however, caused too severity in hematopoietic injury.
  
  
  CONCLUSIONS
  The dose of CTX 100 mg/(kg·d) × 3d is appropriate for leucopenia model of ICR mouse.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Xiong
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Xing
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Shen
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. E-mail: yuzy79@yahoo.com.
authors.forename: Zu-Yin
authors.initials: ZY
authors.lastname: Yu
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D003520:Cyclophosphamide / Q000008:administration & dosage*; D007962:Leukocytes / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D003520:Cyclophosphamide