title: miR-346 Up-regulates Argonaute 2 (AGO2) Protein Expression to Augment the Activity of Other MicroRNAs (miRNAs) and Contributes to Cervical Cancer Cell Malignancy.
abstract: MicroRNAs (miRNAs) are a class of post-transcriptional regulators of gene expression, and AGO2 is essential for miRNA activity. In this study, we focused on the regulation of AGO2 by miR-346 and the consequences in cervical cancer cells. miR-346 enhanced the expression of AGO2, resulting in the increased activity of other miRNAs and contributing to the malignancy of HeLa cells. GRSF1 participated in the regulation of AGO2 by miR-346, and the middle sequence of miR-346 was vital for the synergy effect of miR-346 and GRSF1. We determined that miR-346 promoted the migration and invasion of HeLa cells. In summary, we are the first to report that AGO2 is regulated positively by miRNA and that GRSF1 participates in the miRNA pathway.
authors.affiliation: From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Junfei
authors.initials: J
authors.lastname: Guo
authors.affiliation: From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lv
authors.affiliation: From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: From the Tianjin Life Science Research Center, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China htang2002@yahoo.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D060565:Argonaute Proteins / Q000096:biosynthesis* / Q000235:genetics; D002465:Cell Movement; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D039102:Poly(A)-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: C466422:AGO2 protein, human; D060565:Argonaute Proteins; C088356:GRSF1 protein, human; C560923:MIRN346 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D039102:Poly(A)-Binding Proteins; D012334:RNA, Neoplasm
keywords: AGO2; GRSF1; eukaryotic initiation factor 2 (eIF2); gene activation; gene expression; gene regulation; miR-346; microRNA (miRNA); microRNA mechanism