title: Elucidation of novel budding yeast separase mutants.
abstract: The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.
authors.affiliation: a Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Shimizu
authors.affiliation: a Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Nagai
authors.affiliation: b Faculty of Science, Graduate School of Science , Shizuoka University , Shizuoka , Japan.
authors.forename: Akter M S T
authors.initials: AM
authors.lastname: Yeasmin
authors.affiliation: a Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Koike
authors.affiliation: b Faculty of Science, Graduate School of Science , Shizuoka University , Shizuoka , Japan.
authors.forename: Muhammad Waliullah
authors.initials: MW
authors.lastname: Talukdar
authors.affiliation: a Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.|b Faculty of Science, Graduate School of Science , Shizuoka University , Shizuoka , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushimaru
mesh_terms: D001542:Benomyl / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics; D009154:Mutation; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D064247:Separase / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D001761:Bleomycin; D049452:Green Fluorescent Proteins; D064247:Separase; D001542:Benomyl
keywords: DNA repair; MPT5; Pumilio; securin; separase