title: Monashia flavagen. nov., sp. nov., an actinobacterium of the familyIntrasporangiaceae.
abstract: A novel actinobacterial strain, MUSC 78T, was isolated from a mangrove soil collected from Peninsular Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 78Trepresented a novel lineage within the classActinobacteria. Strain MUSC 78Tformed a distinct clade in the familyIntrasporangiaceaeand was related most closely to members of the generaTerrabacter(98.3-96.8 % 16S rRNA gene sequence similarity),Intrasporangium(98.2-96.8 %),Humibacillus(97.2 %),Janibacter(97.0-95.3 %),Terracoccus(96.8 %),Kribbia(96.6 %),Phycicoccus(96.2-94.7 %),Knoellia(96.1-94.8 %),Tetrasphaera(96.0-94.9 %) andLapillicoccus(95.9 %). Cells were irregular rod-shaped or cocci and stained Gram-positive. The cell-wall peptidoglycan type was A3γ, with ll-diaminopimelic acid as the diagnostic diamino acid. The main cell-wall sugar was mannose and lower amounts of galactose and rhamnose were present. The predominant menaquinone was MK-8(H4). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, diphosphatidylglycerol and phosphoglycolipid. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0and iso-C16 : 0. The DNA G+C content was 73.1 mol%. Based on this polyphasic study, MUSC 78Texhibited phylogenetic and phenotypic differences from members of the genera of the familyIntrasporangiaceae, and therefore a novel species of a new genus,Monashia flavagen. nov., sp. nov., is proposed. The type strain ofMonashia flavais MUSC 78T( = DSM 29621T= MCCC 1K00454T= NBRC 110749T).
authors.affiliation: Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences,Monash University Malaysia, 46150 Bandar Sunway, Selangor Darul Ehsan,Malaysia.
authors.forename: Adzzie-Shazleen
authors.initials: AS
authors.lastname: Azman
authors.affiliation: Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences,Monash University Malaysia, 46150 Bandar Sunway, Selangor Darul Ehsan,Malaysia.|Division of Genetics and Molecular Biology,Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur,Malaysia.
authors.forename: Nurullhudda
authors.initials: N
authors.lastname: Zainal
authors.affiliation: UKM Medical Molecular Biology Institute (UMBI),UKM Medical Centre, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur,Malaysia.
authors.forename: Nurul-Syakima Ab
authors.initials: NA
authors.lastname: Mutalib
authors.affiliation: Division of Genetics and Molecular Biology,Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur,Malaysia.
authors.forename: Wai-Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology,Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur,Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
authors.affiliation: Biomedical Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences,Monash University Malaysia, 46150 Bandar Sunway, Selangor Darul Ehsan,Malaysia.
authors.forename: Learn-Han
authors.initials: LH
authors.lastname: Lee