title: [Protective effect of Danxuetong injection against testicular injury after testis torsion/detorsion in rats].
abstract: OBJECTIVE
  To investigate the protective effect of Danxuetong injection (DXT, a combination of Danshen and Xueshuantong injections) against testicular ischemia-reperfusion injury following testis torsion/detorsion in rats.
  
  
  METHODS
  Thirty-two 4-week-old healthy male SD rats were randomly divided into four groups of equal number: sham operation, normal saline, single DXT injection, and successive DXT injection. The rat models of testicular ischemia-reperfusion injury were established by 2-hour 720-degree torsion/detorsion of the unilateral testis. At 6 weeks after modeling, the rats were killed and their testes were harvested for measure- ment of testicular coefficients, sperm counts, sperm motility, and the levels of total anti-oxidative capacity (T-AOC) , superoxide dismutase (SOD) , nitric oxide synthase (NOS) , and malondialdehyde ( MDA) in the testis tissue.
  
  
  RESULTS
  Compared with the rats of the normal saline group, those of the single DXT injection and successive DXT injection groups showed significant increases in the testicular coefficient (0.11 ± 0.03 vs 0.35 ± 0.04 and 0.40 ± 0.06, P < 0.05), sperm count ([0.46 ± 0.10] vs [1.44 ± 0.50] and [3.00 ± 1.28] x10(9)/ml, P < 0.05), sperm motility ([13.63 ± 14.04] vs [39.63 ± 5.04] and [76.31 ± 3.67]%, P < 0.05), the activity of SOD (72.76 ± 5.58 vs 116.25 ± 8.83 and 133.20 ± 13.84, P < 0.05), and the level of T-AOC (5.58 ± 1.07 vs 13.34 ± 5.81 and 19.21 ± 5.69, P < 0.05), but a remarkable decrease in the content of MDA (42.38 ± 8.94 vs 20.94 ± 5.65 and 15.02 ± 1.03, P < 0. 05) in the injured testes.
  
  
  CONCLUSIONS
  DXT can effectively rid the testis tissue of oxygen free radicals, improve sperm count and motility by antioxidation, and protect the testis tissue of prepubertal rats against testicular ischemia-reperfusion injury after testis torsion/detorsion. It also has a protective effect on the contralateral testis, and successive injection has a better effect than single injection of DXT.
authors.forename: Fei
authors.initials: F
authors.lastname: Yang
authors.forename: Chao-ling
authors.initials: CL
authors.lastname: Yang
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Yu
authors.forename: Zi-feng
authors.initials: ZF
authors.lastname: Yu
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Liu
authors.forename: Pei-yun
authors.initials: PY
authors.lastname: Ni
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D004359:Drug Therapy, Combination / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D027561:Salvia miltiorrhiza; D013086:Spermatic Cord Torsion / Q000150:complications / Q000628:therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000098:blood supply* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; C519374:Panax notoginseng extract; C028972:dan-shen root extract; D008315:Malondialdehyde; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase