title: Tuftsin-derived T-peptide prevents cellular immunosuppression and improves survival rate in septic mice.
abstract: The primary mechanisms of sepsis induced cellular immunosuppression involve immune dysfunction of T lymphocytes and negative immunoregulation of regulatory T cells (Tregs). It has been found that tuftsin is an immune modulating peptide derived from IgG in spleen. T-peptide is one of tuftsin analogs. Herein, we examined the effect of T-peptide on cell-mediated immunity in the presence of lipopolysaccharide (LPS) and the survival rate in septic mice. T-peptide regulated the proliferative ability of CD4(+)CD25(-) T cells in dual responses. Meanwhile, 10 and 100 μg/ml T-peptides were able to enhance the apoptotic rate of CD4(+)CD25(-) T cells compared with 1 μg/ml T-peptide, but markedly lowered interleukin (IL)-2 levels. When CD4(+)CD25(+) Tregs were treated with T-peptide for 24 hours, and co-cultured with normal CD4(+)CD25(-) T cells, the suppressive ability of CD4(+)CD25(+) Tregs on CD4(+)CD25(-) T cells was significantly lowered, along with decreased expression in forkhead/winged helix transcription factor p-3 (Foxp-3) as well as cytotoxic T lymphocyte-associated antigen (CTLA)-4, and secretion of transforming growth factor (TGF)-β. Moreover, T-peptide has the ability to improve outcome of septic mice in a dose- and time- dependent manner, and associated with improvement in the microenvironment of cellular immunosuppression in septic mice.
authors.affiliation: Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.|Trauma Research Center, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Yu-Lei
authors.initials: YL
authors.lastname: Gao
authors.affiliation: Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
authors.forename: Yan-Fen
authors.initials: YF
authors.lastname: Chai
authors.affiliation: Trauma Research Center, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Dong
authors.affiliation: College of Pharmacy, Nankai University, Tianjin 300071, P.R. China.
authors.forename: Su
authors.initials: S
authors.lastname: Han
authors.affiliation: Trauma Research Center, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhu
authors.affiliation: Trauma Research Center, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, P.R. China.
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Zhang
authors.affiliation: Trauma Research Center, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, P.R. China.|State Key Laboratory of Kidney Disease, the Chinese PLA General Hospital, Beijing 100853, P.R. China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Yao
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D060908:CTLA-4 Antigen / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007111:Immunity, Cellular / Q000187:drug effects*; D007155:Immunologic Factors / Q000494:pharmacology*; D056747:Immunomodulation / Q000187:drug effects*; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D051379:Mice; D010641:Phenotype; D018805:Sepsis / Q000188:drug therapy / Q000276:immunology* / Q000401:mortality*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014405:Tuftsin / Q000494:pharmacology*
chemical_list: D000954:Antigens, Surface; D060908:CTLA-4 Antigen; D016207:Cytokines; D051858:Forkhead Transcription Factors; D007155:Immunologic Factors; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D014405:Tuftsin