title: Silica fertilization and nano-MnO₂ amendment on bacterial community composition in high arsenic paddy soils.
abstract: Silica fertilization and nano-MnO2 amendment are reported as useful approaches in lowering the accumulation of arsenic in rice grains, but the effects of silica fertilization or nano-MnO2 amendment on microbial community in the paddy soils containing high concentration of arsenic are still unknown. In order to elucidate this question, the structures and composition of microbial community in the paddy soils, in response to silica fertilization and nano-MnO2 amendment, were investigated using pyrosequencing technique. The results indicated that Proteobacteria, Chloroflexi, and Acidobacteria were the main dominating phyla in these paddy soils. A decrease in the relative abundance of Chloroflexi and Cyanobacteria, but an increase in the relative abundance of Acidobacteria was observed after silica fertilization and nano-MnO2 amendment. The changes of Acidobacteria, Chloroflexi, and Cyanobacteria were strongly correlated with pH and the concentration of bioavailable arsenic in the paddy soils. The α-diversity of bacteria in the paddy soils increased in response to silica fertilization at low amendment level, but decreased under silica or nano-MnO2 amendment at high amendment level. Results of β-diversity analysis indicated that the microbial communities in the control treatment shared more similarity with that of those received low level of nano-MnO2 amendment, and the two silica fertilization treatments also shared more similarity with each other.
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Jihai
authors.initials: J
authors.lastname: Shao
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: He
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Collaborative Innovation Center of Grain and Oil Crops in South China, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Peng
authors.affiliation: Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Agricultural University, Changsha, 410128, People's Republic of China. jdgu@hku.hk.|School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, People's Republic of China. jdgu@hku.hk.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Gu
mesh_terms: D001151:Arsenic / Q000032:analysis; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D058448:Biota / Q000187:drug effects*; D005308:Fertilizers; D017895:Manganese Compounds / Q000008:administration & dosage*; D053758:Nanoparticles / Q000008:administration & dosage*; D012275:Oryza / Q000254:growth & development; D010087:Oxides / Q000008:administration & dosage*; D012822:Silicon Dioxide / Q000008:administration & dosage*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D017895:Manganese Compounds; D010087:Oxides; D012987:Soil; D012822:Silicon Dioxide; D001151:Arsenic; C016552:manganese dioxide
keywords: Arsenic; Bacterial community; Nano-MnO2; Paddy soil; Pyrosequencing; Silica