title: Nanotechnology in Phytotherapy: Antiinflammatory Effect of a Nanostructured Thymol Gel from Lippia sidoides in Acute Periodontitis in Rats.
abstract: Lippia sidoides Cham (Verbenaceae) is largely distributed in the northeastern region of Brazil. It is popularly known as 'Alecrim-pimenta'. Recent studies have shown that some species of Lippia have interesting pharmacological activities. This study aimed to evaluate the effect of a nanostructured thymol gel (TG) 1.2 mg/g on acute phase of ligature-induced periodontitis model [acute periodontal disease (APD)] in rats. APD was induced in 24 Wistar rats subjected to ligature placement on left molars in maxillae. Animals were treated with TG, immediately after APD induction. Saline-based gel was utilized as negative control and diethylammonium diclofenac gel 10 mg/g was used as positive control. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels by ELISA method. TG treatment reduced tissue lesion at histopathology coupled to decreased myeloperoxidase activity production in gingival tissue when compared with the saline gel control group (p < 0.05). The TG gel was able to provide a significant myeloperoxidase decreasing in gingiva tissue confirming to be effective in reducing gingival inflammation in this model.
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.|Department of Surgery, Microsurgery and Medicine Sciences, School of Dentistry, University of Sassari, Sassari, Italy.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Botelho
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Gisele
authors.initials: G
authors.lastname: Barros
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Dinalva B
authors.initials: DB
authors.lastname: Queiroz
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Celso Felício
authors.initials: CF
authors.lastname: Carvalho
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Julia
authors.initials: J
authors.lastname: Gouvea
authors.affiliation: School of Health, University Anhembi-Morumbi, Sao Paulo, SP, Brazil.
authors.forename: Lia
authors.initials: L
authors.lastname: Patrus
authors.affiliation: School of Health, University Anhembi-Morumbi, Sao Paulo, SP, Brazil.
authors.forename: Mariane
authors.initials: M
authors.lastname: Bannet
authors.affiliation: School of Health, University Anhembi-Morumbi, Sao Paulo, SP, Brazil.
authors.forename: Danile
authors.initials: D
authors.lastname: Patrus
authors.affiliation: Post Graduation Program in Biotechnology, University Potiguar, Natal, RN, Brazil.
authors.forename: Amália
authors.initials: A
authors.lastname: Rego
authors.affiliation: Departament of Gynecology, Federal University of São Paulo, Sao Paulo, SP, Brazil.
authors.forename: Ivaldo
authors.initials: I
authors.lastname: Silva
authors.affiliation: Department of Surgery, Microsurgery and Medicine Sciences, School of Dentistry, University of Sassari, Sassari, Italy.
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Campus
authors.affiliation: Departament of Surgery, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Irami
authors.initials: I
authors.lastname: Araújo-Filho
mesh_terms: D000818:Animals; D001938:Brazil; D004195:Disease Models, Animal; D008401:Gas Chromatography-Mass Spectrometry; D005782:Gels / Q000494:pharmacology; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D032411:Lippia / Q000737:chemistry*; D008297:Male; D036103:Nanotechnology; D009504:Neutrophils / Q000166:cytology; D009822:Oils, Volatile / Q000737:chemistry; D010518:Periodontitis / Q000188:drug therapy*; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013943:Thymol / Q000494:pharmacology*
chemical_list: D005782:Gels; D009822:Oils, Volatile; D010938:Plant Oils; D013943:Thymol; D009195:Peroxidase
keywords: Brazilian plants; Lippia sidoides; Verbenacea; nanotechnology; periodontitis; thymol