title: Production, structural characterization and gel forming property of a new exopolysaccharide produced by Agrobacterium HX1126 using glycerol or d-mannitol as substrate.
abstract: A strain Agrobacterium HX1126 was isolated from soil sample near the canal in Wuxi. Glycerol was used as carbon source for the production of a new exopolysaccharide which was named PGHX. PGHX composed mainly of galactose, with lower amounts of arabinose and aminogalactose. It was found that this strain could use d-mannitol as carbon source to produce PGHX too. A method for the preparation of crude PGHX was proposed and the crude PGHX can be formed in a gel formation when 30 g/L was put into the boiling water for 10 min, with an achieved gel strength of 957 g/cm(2). The concentration of proteins in the crude product was considered to be an important parameter which directly influence the gel forming property. The highest production of PGHX (24.9 g/L) was obtained under the nitrogen depletion condition. The structure of the product was confirmed by NMR and FTIR.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: liuym82@163.com.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Qiuya
authors.initials: Q
authors.lastname: Gu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Fred Kwame
authors.initials: FK
authors.lastname: Ofosu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: princess090326@163.com.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Yu
mesh_terms: D060054:Agrobacterium / Q000378:metabolism*; D005782:Gels / Q000737:chemistry*; D007218:Industrial Microbiology / Q000379:methods; D008353:Mannitol / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D013718:Tensile Strength
chemical_list: D005782:Gels; D011135:Polysaccharides, Bacterial; D008353:Mannitol
keywords: Agrobacterium HX1126; Exopolysaccharide; Gel forming property; Glycerol; d-Mannitol