title: Characterization of Recombinant Human Cytomegaloviruses Encoding IE1 Mutants L174P and 1-382 Reveals that Viral Targeting of PML Bodies Perturbs both Intrinsic and Innate Immune Responses.
abstract: PML is the organizer of cellular structures termed nuclear domain 10 (ND10) or PML-nuclear bodies (PML-NBs) that act as key mediators of intrinsic immunity against human cytomegalovirus (HCMV) and other viruses. The antiviral function of ND10 is antagonized by viral regulatory proteins such as the immediate early protein IE1 of HCMV. IE1 interacts with PML through its globular core domain (IE1CORE) and induces ND10 disruption in order to initiate lytic HCMV infection. Here, we investigate the consequences of a point mutation (L174P) in IE1CORE, which was shown to abrogate the interaction with PML, for lytic HCMV infection. We found that a recombinant HCMV encoding IE1-L174P displays a severe growth defect similar to that of an IE1 deletion virus. Bioinformatic modeling based on the crystal structure of IE1CORE suggested that insertion of proline into the highly alpha-helical domain severely affects its structural integrity. Consistently, L174P mutation abrogates the functionality of IE1CORE and results in degradation of the IE1 protein during infection. In addition, our data provide evidence that IE1CORE as expressed by a recombinant HCMV encoding IE1 1-382 not only is required to antagonize PML-mediated intrinsic immunity but also affects a recently described function of PML in innate immune signaling. We demonstrate a coregulatory role of PML in type I and type II interferon-induced gene expression and provide evidence that upregulation of interferon-induced genes is inhibited by IE1CORE. In conclusion, our data suggest that targeting PML by viral regulatory proteins represents a strategy to antagonize both intrinsic and innate immune mechanisms.
  
  
  OBJECTIVE
  PML nuclear bodies (PML-NBs), which represent nuclear multiprotein complexes consisting of PML and additional proteins, represent important cellular structures that mediate intrinsic resistance against many viruses, including human cytomegalovirus (HCMV). During HCMV infection, the major immediate early protein IE1 binds to PML via a central globular domain (IE1CORE), and we have shown previously that this is sufficient to antagonize intrinsic immunity. Here, we demonstrate that modification of PML by IE1CORE not only abrogates intrinsic defense mechanisms but also attenuates the interferon response during infection. Our data show that PML plays a novel coregulatory role in type I as well as type II interferon-induced gene expression, which is antagonized by IE1CORE. Importantly, our finding supports the view that targeting of PML-NBs by viral regulatory proteins has evolved as a strategy to inhibit both intrinsic and innate immune defense mechanisms.
authors.affiliation: Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Myriam
authors.initials: M
authors.lastname: Scherer
authors.affiliation: Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Victoria
authors.initials: V
authors.lastname: Otto
authors.affiliation: Division of Bioinformatics, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Joachim D
authors.initials: JD
authors.lastname: Stump
authors.affiliation: Division of Biotechnology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Klingl
authors.affiliation: Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Müller
authors.affiliation: Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Reuter
authors.affiliation: Division of Biotechnology, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Yves A
authors.initials: YA
authors.lastname: Muller
authors.affiliation: Division of Bioinformatics, Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Sticht
authors.affiliation: Institute for Clinical and Molecular Virology, Universität Erlangen-Nürnberg, Erlangen, Germany thomas.stamminger@viro.med.uni-erlangen.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Stamminger
mesh_terms: D019295:Computational Biology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007113:Immunity, Innate; D008958:Models, Molecular; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D017354:Point Mutation; D000071156:Promyelocytic Leukemia Protein; D011487:Protein Conformation; D017384:Sequence Deletion; D014157:Transcription Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: C088645:IE1 protein, cytomegalovirus; D017874:Immediate-Early Proteins; D050505:Mutant Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human