title: Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds.
abstract: Cell-based systems are useful for discovering antiviral agents. Dissecting the viral life cycle, particularly the early entry stages, allows a mechanistic approach to identify and evaluate antiviral agents that target specific steps of the viral entry. In this report, the methods of examining viral inactivation, viral attachment, and viral entry/fusion as antiviral assays for such purposes are described, using hepatitis C virus as a model. These assays should be useful for discovering novel antagonists/inhibitors to early viral entry and help expand the scope of candidate antiviral agents for further drug development.
authors.affiliation: Department of Chinese Medicine, Taipei Medical University Hospital; Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University.
authors.forename: Chen-Jei
authors.initials: CJ
authors.lastname: Tai
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University.
authors.forename: Chia-Lin
authors.initials: CL
authors.lastname: Li
authors.affiliation: Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University.
authors.forename: Cheng-Jeng
authors.initials: CJ
authors.lastname: Tai
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University; Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital.
authors.forename: Chien-Kai
authors.initials: CK
authors.lastname: Wang
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University; Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University; ltlin@tmu.edu.tw.
authors.forename: Liang-Tzung
authors.initials: LT
authors.lastname: Lin
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D053586:Virus Internalization / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents