title: An AhR-Luciferase Adenovirus Infection System for Rapid Screening of Dioxins in Soils.
abstract: Our goal was to develop a fast-screening method for measuring dioxin levels in soils. The adenovirus (Ad)-dioxin-responsive (DR) bioassay system (AdEasy-6XDRE-TATA-Luc) combined with a fast-cleanup system was examined under conventional conditions (i.e., with incubation at 37°C) and three alternative conditions [incubation at 37°C with addition of phorbol-12-myristate-13-acetate (PMA), incubation at 33°C, and incubation at 33°C with addition of PMA]. The best conditions for carrying out the Ad-DR bioassay was 33°C and no addition of PMA. The background level of soil dioxins determined by the chemical assay [6.49 ng I-TEQ/kg dry weight (dw)] was well correlated (Pearson's r = 0.873, p < 0.001) with that by the Ad-DR bioassay [expressed in ng bioanalytical equivalents (BEQ) 81.1 ng BEQ/kg dw] (n = 17). When surveyed in contaminated soil samples (n = 114) from industrial areas by the Ad-DR bioassay, dioxin levels were 117, 102, 98.5, and 112 ng BEQ/kg dw, respectively, in northern, central, southern, and eastern Taiwan.
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, 1 Shuefu Road, Neipu, Pingtung County, 912, Taiwan.|Department of Radiology, Pingtung Christian Hospital, Pingtung City, Pingtung, 900, Taiwan.|Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, 20 Weixin Road, Yanpu, Pingtung County, 907, Taiwan.
authors.forename: Cherng-Gueih
authors.initials: CG
authors.lastname: Shy
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, 1 Shuefu Road, Neipu, Pingtung County, 912, Taiwan. hrchao@mail.npust.edu.tw.
authors.forename: How-Ran
authors.initials: HR
authors.lastname: Chao
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, 1 Shuefu Road, Neipu, Pingtung County, 912, Taiwan.
authors.forename: Ding-Yan
authors.initials: DY
authors.lastname: Lin
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, 1 Shuefu Road, Neipu, Pingtung County, 912, Taiwan.
authors.forename: Yan-You
authors.initials: YY
authors.lastname: Gou
authors.affiliation: Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu City, 300, Taiwan.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Chuang
authors.affiliation: Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung County, 912, Taiwan.
authors.forename: Kuo Pin
authors.initials: KP
authors.lastname: Chuang
authors.affiliation: Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, 1 Shuefu Road, Neipu, Pingtung County, 912, Taiwan.
authors.forename: Yung-Chun
authors.initials: YC
authors.lastname: Hsiao
authors.affiliation: Department of Pharmacy, Graduate Institute of Pharmaceutical Technology, Tajen University, 20 Weixin Road, Yanpu, Pingtung County, 907, Taiwan. fachen@tajen.edu.tw.
authors.forename: Fu-An
authors.initials: FA
authors.lastname: Chen
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004147:Dioxins / Q000032:analysis* / Q000737:chemistry; D004784:Environmental Monitoring / Q000379:methods*; D017930:Genes, Reporter; D008156:Luciferases / Q000737:chemistry; D051381:Rats; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry; D013624:Taiwan
chemical_list: D004147:Dioxins; D012987:Soil; D012989:Soil Pollutants; D008156:Luciferases
keywords: Ad-DR bioassay; Aryl hydrocarbon receptor (AhR); Dioxins; Luciferase; Soil