title: Effect of GABA on blood pressure and blood dynamics of anesthetic rats.
abstract: BACKGROUND
  This study aimed to investigate GABA effects on blood pressure and blood dynamics of anesthetic rats by observing spontaneously hypertensive rats under both anesthesia and waking state.
  
  
  METHODS
  72 male waking Wistar-Kyokos (WKY) rats and 72 male anesthetized spontaneously hypertensive (SHR) rats were randomly divided into control group and experimental group (N = 36 each). Rats were further divided into three subgroups (N = 12 each), which received 15 μmol GABA, 35 nmol muscimol, or 4 nmol dicentrine into unilateral paraventricular nucleus, respectively. Rats in the control group (WKY1) and experimental group (SHR1) were compared for the GABA effect on blood pressure (MAP), heart rate (HR), and arterial baroreceptor reflex function (BRS) changes under waking state. Anesthetic WKY rats (WKY2) and spontaneously hypertensive rats (SHR2) were compared for the GABA effect on those abovementioned indexes. Abdominal aorta mean arterial pressure, heart rate, and arterial baroreceptor reflex function changes were compared in all rats.
  
  
  RESULTS
  MAP, HR, and BRS were slightly lower in the rats under anesthetic state than in waking state before treatment (P < 0.05); they did not show significant changes between anesthetic and waking state, however, after treatment (P > 0.05). Unilateral paraventricular nucleus injection of GABA or muscimol elevated MAP, HR, and BRS in both normal and spontaneously hypertensive rats under waking or anesthetic state (P < 0.05). In addition, the amplitudes of changes of three indicators in spontaneously hypertensive group were markedly higher than those of control group (P < 0.05). Dicentrine could induce MAP and HR to increase, while BRS decreased significantly (P < 0.05). The amplitudes of changes in spontaneously hypertensive group were larger than those of normal group (P < 0.05).
  
  
  CONCLUSIONS
  Centrally GABA injection can enhance the BRS function in spontaneously hypertensive rats and adjust heart rate to reduce the blood pressure fluctuation. It may play a role in reducing blood pressure and protecting cardiovascular function.
authors.affiliation: Department of Anesthesiogy, Anqiu People's Hospital Anqiu 262100, China.
authors.forename: Pengju
authors.initials: P
authors.lastname: Ma
authors.affiliation: Delivery Room, People's Hospital of Anqiu Anqiu 262100, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Anesthesiogy, Weifang People's Hospital Weifang 261041, China.
authors.forename: Fanceng
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Critical Care Medicine of Weifang People's Hospital Weifang 261041, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Critical Care Medicine of Weifang People's Hospital Weifang 261041, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Pang
keywords: arterial baroreceptor reflex function; blood dynamics; blood pressure; cardiovascular; γ-aminobutiric acid