title: Expressional profiles of transcription factors in the progression of Helicobacter pylori-associated gastric carcinoma based on protein/DNA array analysis.
abstract: Transcription factors (TFs) are crucial modulators of gene expression during the development and progression of gastric carcinoma. Helicobacter pylori (H. pylori) is one of the most significant risk factors of gastric carcinoma, and it is widely known that chronic inflammation with H. pylori infection triggers gastric carcinogenesis through inflammation-carcinoma chain [gastric carcinogenesis stages: non-atrophic gastritis, chronic atrophic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma (GC)], but its mechanism regarding changed TFs remains unknown. In this study, we investigated the expressional profiles of 345 transcription factors in gastric mucosa of healthy volunteers and patients at different gastric carcinogenesis stages using protein/DNA array-based approach. The data demonstrated the up-regulated TFs such as GATA-3, AP4, c-Myc and Pbx1 in the gastric mucosa of GC patients compared with the healthy volunteers, while other TFs, particularly CCAAT and CACC, showed the consistently decreasing trend along the gastric carcinogenesis. The increased expressions of AP4, Pbx1 and C/EBPα were further validated by quantitative real-time PCR and Western blot in various H. pylori-infected models such as clinical gastric tissues, gastric epithelial cell lines and Mongolian gerbils. This study provides insights into and potential laws for gene transcriptional regulation by identifying potential TFs targets against the development of H. pylori-associated gastric carcinoma.
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Ting-Zi
authors.initials: TZ
authors.lastname: Hu
authors.affiliation: Center for Medical Experiment, Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan Province, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China. xucanxia2000@163.com.|Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Changsha, 410013, China. xucanxia2000@163.com.
authors.forename: Can-Xia
authors.initials: CX
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Liu
authors.affiliation: Department of Internal Medicine, The Third People's Hospital of Huaihua, Huaihua, 418000, Hunan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital of Central South University, 138 Tongzipo Street, Changsha, 410013, Hunan Province, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Jiang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods*; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D054884:Host-Pathogen Interactions / Q000235:genetics*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D013274:Stomach Neoplasms / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors
keywords: AP4; C/EBPα; Gastric carcinogenesis; Helicobacter pylori; Pbx1; Protein/DNA array analysis; Transcription factors