title: The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan.
abstract: In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Iguchi
authors.forename: Takehisa
authors.initials: T
authors.lastname: Soma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suzuki
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D053626:Atovaquone / Q000494:pharmacology*; D001403:Babesia / Q000187:drug effects* / Q000235:genetics; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D004351:Drug Resistance / Q000235:genetics; D062665:Epidemiological Monitoring; D005260:Female; D007564:Japan / Q000453:epidemiology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000977:Antiparasitic Agents; D053626:Atovaquone