title: Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability.
abstract: Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species.
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. xuj@mail.sceuc.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. houfuyuany@sina.com.
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Hou
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. wandr666@163.com.
authors.forename: Ding-Rong
authors.initials: DR
authors.lastname: Wan
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. shaqueen@sina.com.
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. xudongmei777@sina.com.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Xu
authors.affiliation: College of Pharmacy, South Central University for Nationalities, 708 Minyuan Road, Wuhan 430074, China. yanggz888@126.com.
authors.forename: Guang-Zhong
authors.initials: GZ
authors.lastname: Yang
mesh_terms: D003433:Crosses, Genetic; D014644:Genetic Variation; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D031963:Sedum / Q000235:genetics*
keywords: Sedum sarmentosum; genetic diversity; microsatellite markers; polymorphism; transferability