title: Single-particle cryo-EM data acquisition by using direct electron detection camera.
abstract: Recent advances in single-particle electron cryo-microscopy (cryo-EM) were largely facilitated by the application of direct electron detection cameras. These cameras feature not only a significant improvement in detective quantum efficiency but also a high frame rate that enables images to be acquired as 'movies' made of stacks of many frames. In this review, we discuss how the applications of direct electron detection cameras in cryo-EM have changed the way the data are acquired.
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Shenping
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Armache
authors.affiliation: Department of Biochemistry and Biophysics, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA Howard Hughes Medical Institute, University of California San Francisco, 600 16th Street, San Francisco, CA 94158, USA ycheng@ucsf.edu.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D004583:Electrons; D021621:Imaging, Three-Dimensional / Q000379:methods*
keywords: direct electron detection camera; electron cryo-microscopy; single-particle cryo-EM