title: Low T3 State Is Correlated with Cardiac Mitochondrial Impairments after Ischemia Reperfusion Injury: Evidence from a Proteomic Approach.
abstract: Mitochondria are major determinants of cell fate in ischemia/reperfusion injury (IR) and common effectors of cardio-protective strategies in cardiac ischemic disease. Thyroid hormone homeostasis critically affects mitochondrial function and energy production. Since a low T3 state (LT3S) is frequently observed in the post infarction setting, the study was aimed to investigate the relationship between 72 h post IR T3 levels and both the cardiac function and the mitochondrial proteome in a rat model of IR. The low T3 group exhibits the most compromised cardiac performance along with the worst mitochondrial activity. Accordingly, our results show a different remodeling of the mitochondrial proteome in the presence or absence of a LT3S, with alterations in groups of proteins that play a key role in energy metabolism, quality control and regulation of cell death pathways. Overall, our findings highlight a relationship between LT3S in the early post IR and poor cardiac and mitochondrial outcomes, and suggest a potential implication of thyroid hormone in the cardio-protection and tissue remodeling in ischemic disease.
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. simona@ifc.cnr.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Forini
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. nucciferri@ifc.cnr.it.
authors.forename: Nadia
authors.initials: N
authors.lastname: Ucciferri
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. kusmic@ifc.cnr.it.
authors.forename: Claudia
authors.initials: C
authors.lastname: Kusmic
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. nicolini@ifc.cnr.it.|Fondazione Toscana Gabriele Monasterio, Via Giuseppe Moruzzi 1, Pisa 56124, Italy. nicolini@ifc.cnr.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Nicolini
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa, Via Volta Pisa 56124, Italy. acecch@biomed.unipi.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Cecchettini
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. silvia.rocchiccioli@ifc.cnr.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rocchiccioli
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. lorenzo.citti@ifc.cnr.it.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Citti
authors.affiliation: Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology,Via Giuseppe Moruzzi 1, Pisa 56124, Italy. iervasi@ifc.cnr.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Iervasi
mesh_terms: D000818:Animals; D016923:Cell Death / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D008929:Mitochondria, Heart / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009203:Myocardial Infarction / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D014284:Triiodothyronine / Q000172:deficiency / Q000235:genetics*
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome; D014284:Triiodothyronine
keywords: ischemia/reperfusion; low T3 state; mitochondria proteomic profiling; mitochondrial function