title: Follicular dendritic cell-secreted protein may enhance osteoclastogenesis in periodontal disease.
abstract: OBJECTIVE
  Follicular dendritic cell-secreted protein (FDC-SP) has been found to be expressed in periodontal ligament (PDL), a layer of soft connective tissue between tooth root and alveolar bone, and involved in immunoreaction. This study was performed to explore the potential role of FDC-SP in periodontal disease.
  
  
  METHODS
  The human periodontal ligament cells (hPDLCs) were stimulated with Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) and FDC-SP expression was examined by real-time PCR and western blot. Then this molecule was overexpressed or silenced in hPDLCs by transfection of FDC-SP expression plasmids or its small-interfering (si) RNA, respectively, and the effects of FDC-SP on expression of osteogenesis- and osteoclastogenesis-related genes in hPDLCs were analyzed by real-time PCR and western blot.
  
  
  RESULTS
  Our results showed that P. gingivalis LPS upregulated FDC-SP expression in hPDLCs. Overexpression of FDC-SP could decrease the expression of osteogenesis-related genes, increase the expression of osteoclastogenesis-related genes and RANKL/OPG ratio in hPDLCs. Meanwhile, silence of FDC-SP expression in hPDLCs remarkably inversed the above results.
  
  
  CONCLUSIONS
  LPS-induced upregulation of FDC-SP expression in hPDLCs may enhance osteoclastogenesis in periodontal disease.
authors.affiliation: a Department of Periodontology , Peking University School and Hospital of Stomatology , Beijing , China.
authors.forename: Jianru
authors.initials: J
authors.lastname: Liu
authors.affiliation: b Department of Stomatology , the First Affiliated Hospital of the Chinese PLA General Hospital , Beijing , China.
authors.forename: Huan
authors.initials: H
authors.lastname: Bian
authors.affiliation: c Department of VIP dental service , Peking University School and Hospital of Stomatology , Beijing , China.
authors.forename: Ruiyu
authors.initials: R
authors.lastname: Ding
authors.affiliation: c Department of VIP dental service , Peking University School and Hospital of Stomatology , Beijing , China.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Chi
authors.affiliation: d Central laboratory , Peking University School and Hospital of Stomatology , Beijing , China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010010:Osteoclasts / Q000378:metabolism* / Q000473:pathology; D010012:Osteogenesis; D010510:Periodontal Diseases / Q000378:metabolism* / Q000473:pathology; D010513:Periodontal Ligament / Q000378:metabolism* / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000737:chemistry; D011506:Proteins / Q000378:metabolism*
chemical_list: C465580:FDCSP protein, human; D008070:Lipopolysaccharides; D011506:Proteins
keywords: FDC-SP; lipopolysaccharide; osteoclastogenesis; osteogenesis; periodontal ligament cells