title: Cysteine-Rich Peptide Family with Unusual Disulfide Connectivity from Jasminum sambac.
abstract: Cysteine-rich peptides (CRPs) are natural products with privileged peptidyl structures that represent a potentially rich source of bioactive compounds. Here, the discovery and characterization of a novel plant CRP family, jasmintides from Jasminum sambac of the Oleaceae family, are described. Two 27-amino acid jasmintides (jS1 and jS2) were identified at the gene and protein levels. Disulfide bond mapping of jS1 by mass spectrometry and its confirmation by NMR spectroscopy revealed disulfide bond connectivity of C-1-C-5, C-2-C-4, and C-3-C-6, a cystine motif that has not been reported in plant CRPs. Structural determination showed that jS1 displays a well-defined structure framed by three short antiparallel β-sheets. Genomic analysis showed that jasmintides share a three-domain precursor arrangement with a C-terminal mature domain preceded by a long pro-domain of 46 residues and an intron cleavage site between the signal sequence and pro-domain. The compact cysteine-rich structure together with an N-terminal pyroglutamic acid residue confers jasmintides high resistance to heat and enzymatic degradation, including exopeptidase treatment. Collectively, these results reveal a new plant CRP structure with an unusual cystine connectivity, which could be useful as a scaffold for designing peptide drugs.
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Geeta
authors.initials: G
authors.lastname: Kumari
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Aida
authors.initials: A
authors.lastname: Serra
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Joon
authors.initials: J
authors.lastname: Shin
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Phuong Q T
authors.initials: PQ
authors.lastname: Nguyen
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Siu Kwan
authors.initials: SK
authors.lastname: Sze
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Ho Sup
authors.initials: HS
authors.lastname: Yoon
authors.affiliation: School of Biological Sciences, Nanyang Technological University , 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: James P
authors.initials: JP
authors.lastname: Tam
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids; D003545:Cysteine / Q000737:chemistry*; D003553:Cystine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D031660:Jasminum / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D029592:Oleaceae / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017434:Protein Structure, Tertiary
chemical_list: D000596:Amino Acids; D004220:Disulfides; D010455:Peptides; D010940:Plant Proteins; C000605762:jasmintide jS1; D003553:Cystine; D003545:Cysteine