title: Highly Sensitive LC-MS-MS Method for the Determination of Tacrine in Rat Plasma: Application to Pharmacokinetic Studies in Rats.
abstract: A rapid and highly sensitive assay method has been developed and validated for the estimation of tacrine in rat plasma using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The assay procedure involves a simple liquid-liquid extraction of tacrine and phenacetin (internal standard, IS) from rat plasma using ethyl acetate. Chromatographic separation was achieved with 0.2% formic acid : acetonitrile (30 : 70, v/v) at a flow rate of 0.50 mL/min on an Atlantis dC18 column with a total run time of 3.0 min. The MS-MS ion transitions monitored were 199.10 → 171.20 for tacrine and 180.10 → 110.10 for IS. Method validation was performed as per United States Food and Drug Administration (US FDA) guidelines and the results met the acceptance criteria. The lower limit of quantification achieved was 0.008 ng/mL and linearity was observed from 0.008 to 53.4 ng/mL. The intra- and inter-day precision was in the range of 2.76-12.5 and 5.15-12.8%, respectively. This novel method has been applied to a pharmacokinetic study in rats.
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Vels University, Chennai 600117, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Ponnayyan Sulochana
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Vels University, Chennai 600117, India.
authors.forename: Vishnuvardh
authors.initials: V
authors.lastname: Ravichandiran
authors.affiliation: Jubilant Biosys Ltd, Industrial Suburb, Yeshwanthpur, Bangalore 560 022, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Mullangi
authors.affiliation: Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Vels University, Chennai 600117, India sathesh2000@gmail.com.
authors.forename: Sathesh Kumar
authors.initials: SK
authors.lastname: Sukumaran
mesh_terms: D000085:Acetates; D000097:Acetonitriles; D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards*; D005561:Formates; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D018697:Nootropic Agents / Q000097:blood / Q000493:pharmacokinetics*; D015588:Observer Variation; D010615:Phenacetin / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods / Q000592:standards*; D013619:Tacrine / Q000097:blood / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards*
chemical_list: D000085:Acetates; D000097:Acetonitriles; D005561:Formates; D018697:Nootropic Agents; D012997:Solvents; C030544:formic acid; D013619:Tacrine; C007650:ethyl acetate; D010615:Phenacetin; C032159:acetonitrile