title: Functional variants inPXRare associated with colorectal cancer susceptibility in Chinese populations.
abstract: BACKGROUND
  As an important member of the steroid nuclear receptor family, recent research has suggested that PXR may play important roles in the development of multiple cancers. However, no well-designed studies has been conducted to investigate the associations between genetic polymorphisms of PXR and colorectal cancer (CRC) risk in Chinese populations.
  
  
  METHODS
  We performed a hospital-based case-control analysis to assess two genetic polymorphisms in the 3'-untranslated regions (3'-UTR) via allele-specific MALDI-TOF mass spectrometry assay and evaluated the associations between two polymorphisms and risk of CRC.
  
  
  RESULTS
  The PXR rs3814058C>T polymorphism was significantly associated with a higher risk of CRC (P<10-3), and the CT/TT variant genotypes had an increased CRC risk (adjusted odds ratio=1.54, 95% confidence interval=1.27-1.83) comparing CC genotype. In stratified analyses, rs3814058CT+TT genotypes was associated with increased risk among alcohol consumers (P=0.002). In vitro experiments indicated that the rs3814058C to rs3814058T transition gained a new binding of the microRNA hsa-miR-129-5p and decreased the PXR expression.
  
  
  CONCLUSIONS
  Our data suggest that the functional polymorphism rs3814058C>T in 3'-UTR of PXR may be a functional biomarker to predict risk of CRC.
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Bofeng
authors.initials: B
authors.lastname: Su
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Lemen
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Endoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Xueyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Operating Room, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
authors.forename: Xixia
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Endoscopic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China. Electronic address: wz1370666@126.com.
authors.forename: Xiangjian
authors.initials: X
authors.lastname: Chen
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016017:Odds Ratio; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020641:Polymorphism, Single Nucleotide; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C541915:Mirn129 microRNA, human; D000074435:Peroxisome-Targeting Signal 1 Receptor; D018160:Receptors, Cytoplasmic and Nuclear
keywords: Colorectal cancer; PXR; SNP