title: Evaluation of the Post-Mortem Superoxide Dismutase, Glutathione Peroxidase Activities and Malondialdehyde Level in Renal and Brain Tissues: Is it Possible to Estimate Post-Mortem Interval Using these Parameters?
abstract: BACKGROUND
  Estimation of the postmortem interval (PMI) is one major area of interest in forensic medicine. We investigated changes of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels in rat brain and renal tissues in the PMI and examined the potential changes which may be useful for an estimation of time of death.
  
  
  METHODS
  Forty albino female rats were used in this study. The rats were sacrificed and divided into five groups: Group 1: 0 hour, Group 2: 6 hours, Group 3: 12 hours, Group 4: 24 hours, and Group 5: 48 hours. The rats were housed at room temperature (22 degrees C ± 2 degrees C) for the defined period for each group, and their brains and kidneys were excised.
  
  
  RESULTS
  The postmortem MDA levels were significantly different between groups for both renal and brain tissues (p = 0.003; p = 0.007). The diagnostic adequacy of MDA was calculated. Regarding SOD and GSH-Px, we were not able to obtain any date that would be useful for the prediction of PMI.
  
  
  CONCLUSIONS
  According to this study, it is possible that an estimation of the PMI can be made using postmortem MDA levels. Regarding SOD and GSH-Px, we were not able to obtain any data that would be useful for the prediction of PMI.
authors.forename: Burak
authors.initials: B
authors.lastname: Gümüş
authors.forename: Alper
authors.initials: A
authors.lastname: Gümüş
authors.forename: Ali
authors.initials: A
authors.lastname: Yildirim
authors.forename: Erdal
authors.initials: E
authors.lastname: Ozer
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Ozyurt
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sahin
authors.forename: Macit
authors.initials: M
authors.lastname: Koldaş
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis*; D007668:Kidney / Q000737:chemistry*; D008315:Malondialdehyde / Q000032:analysis*; D011180:Postmortem Changes; D012372:ROC Curve; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000032:analysis*; D013696:Temperature; D013997:Time Factors
chemical_list: D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase