title: Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis.
abstract: PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans. In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L. lactis while under stress. PprI was stably expressed in L. lactis as confirmed by western blot assays. L. lactis expressing PprI exhibited significantly improved resistance to oxidative stress and high osmotic pressure. This enhanced cellular tolerance to stressors might be due to the regulation of resistance-related genes (e.g., recA, recO, sodA, and nah) by pprI. Moreover, transformed L. lactis demonstrated increased lactic acid production, attributed to enhanced lactate dehydrogenase activity. These results suggest that pprI can improve the tolerance of L. lactis to environmental stresses, and this transformed bacterial strain is a promising candidate for industrial applications of lactic acid production.
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Tian
authors.affiliation: Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Shang
authors.initials: S
authors.lastname: Dai
authors.affiliation: Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Linna
authors.initials: L
authors.lastname: Guo
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, China.
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Hua
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D034301:Deinococcus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D007218:Industrial Microbiology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D009997:Osmotic Pressure; D018384:Oxidative Stress; D014170:Transformation, Genetic
chemical_list: D001426:Bacterial Proteins; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase