title: [Protective effect of Sanhuangyinchi Fang drug serum on hydrogen peroxide-induced DNA oxidative damage in LO2 cells].
abstract: OBJECTIVE
  To study the protective effect of Sanhuangyinchi Fang drug serum (SF) against hydrogen peroxide-mediated DNA oxidative damage in LO2 cells.
  
  
  METHODS
  The LO2 cells were randomly divided into the control group, H(2)O(2) group, SF groups (5%, 10%, and 15%) and vitE group. The morphological features of the treated LO2 cells were observed under inverted microscope. The viability of the treated cells was assessed with CCK-8 method, and the activity of SOD, CAT and GSH-PX were detected biochemically. Reactive oxygen species (ROS) levels, the content of 8-OHdG, and DNA damage of the cells were evaluated by flow cytometry, ELISA, and Comet assay, respectively.
  
  
  RESULTS
  Compared with H(2)O(2) group, the cells in SF groups (10% and 15%) and vitE group showed higher cell survival rate (P<0.05) and higher SOD, CAT, GSH-PX (P<0.05) and ROS scavenging activities (P<0.01) with markedly decreases the content of 8-OHdG (P<0.01) and reduced tailing ratio, tail length, tail moment and Olive tail moment (P<0.05).
  
  
  CONCLUSIONS
  SF drug serum, especially at the concentration of 15%, can protect LO2 cells from H(2)O(2)-mediated DNA oxidative damage.
authors.affiliation: Southern Medical University, School of Traditional Chinese Medicine, Guangzhou 510515, China. E-mail: 504614883@qq.com.
authors.forename: Huan
authors.initials: H
authors.lastname: Dai
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Diao
authors.forename: Jin-Ying
authors.initials: JY
authors.lastname: Ou
authors.forename: Hai-Ye
authors.initials: HY
authors.lastname: Li
authors.forename: Yun-Gao
authors.initials: YG
authors.lastname: Yang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine