title: Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish.
abstract: Although atherosclerosis is a multifactorial disease, the role of hemodynamic information has become more important. Low and oscillating wall shear stress (WSS) that changes its direction is associated with the early stage of atherosclerosis. Several in vitro and in vivo models were proposed to reveal the relation between the WSS and the early atherosclerosis. However, these models possess technical limitations in mimicking real physiological conditions and monitoring the developmental course of the early atherosclerosis. In this study, a hypercholesterolaemic zebrafish model is proposed as a novel experimental model to resolve these limitations. Zebrafish larvae are optically transparent, which enables temporal observation of pathological variations under in vivo condition. WSS in blood vessels of 15 days post-fertilisation zebrafish was measured using a micro particle image velocimetry (PIV) technique, and spatial distribution of lipid deposition inside the model was quantitatively investigated after feeding high cholesterol diet for 10 days. Lipids were mainly deposited in blood vessel of low WSS. The oscillating WSS was not induced by the blood flows in zebrafish models. The present hypercholesterolaemic zebrafish would be used as a potentially useful model for in vivo study about the effects of low WSS in the early atherosclerosis.
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang 790-784, Republic of Korea.
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang 790-784, Republic of Korea.
authors.forename: Woorak
authors.initials: W
authors.lastname: Choi
authors.affiliation: Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Dalseong, Daegu 711-873, Republic of Korea.
authors.forename: Eunseok
authors.initials: E
authors.lastname: Seo
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang 790-784, Republic of Korea.
authors.forename: Eunseop
authors.initials: E
authors.lastname: Yeom
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000209:etiology* / Q000473:pathology* / Q000503:physiopathology; D001808:Blood Vessels / Q000473:pathology*; D004195:Disease Models, Animal; D006439:Hemodynamics; D006937:Hypercholesterolemia / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D008055:Lipids / Q000032:analysis; D011673:Pulsatile Flow; D013314:Stress, Mechanical; D015027:Zebrafish
chemical_list: D008055:Lipids