title: Raman spectroscopy-based identification of nosocomial outbreaks of the clonal bacterium Escherichia coli.
abstract: DNA-based techniques are frequently used to confirm the relatedness of putative outbreak isolates. These techniques often lack the discriminatory power when analyzing closely related microbes such as E. coli. Here the value of Raman spectroscopy as a typing tool for E. coli in a clinical setting was retrospectively evaluated.
authors.affiliation: Department of Medical Microbiology, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands. h.kusters@umcutrecht.nl.
authors.forename: J G
authors.initials: JG
authors.lastname: Kusters
authors.affiliation: Department of Innovative Molecular Diagnostics, University of Applied Sciences Leiden, Leiden, The Netherlands.
authors.forename: W B
authors.initials: WB
authors.lastname: van Leeuwen
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Maquelin
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: H E M
authors.initials: HE
authors.lastname: Blok
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: H F M
authors.initials: HF
authors.lastname: Willemse
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: L A M
authors.initials: LA
authors.lastname: de Graaf-Miltenburg
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: A C
authors.initials: AC
authors.lastname: Fluit
authors.affiliation: Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Troelstra
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D004926:Escherichia coli / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D012189:Retrospective Studies; D013059:Spectrum Analysis, Raman / Q000379:methods*