title: Polymerase Chain Reaction in the Diagnosis of Visceral Leishmaniasis Recurrence in the Setting of Negative Splenic Smears.
abstract: This report presents two cases of visceral leishmaniasis (VL) recurrence where the microscopy of the splenic smear failed in diagnosis. However, a strong clinical suspicion compelled further evaluation by polymerase chain reaction (PCR), which validated the etiology. This short report highlights the usefulness of PCR in diagnosing cases of suspected smear-negative VL recurrence.
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Golam
authors.initials: G
authors.lastname: Hasnain
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Ariful
authors.initials: A
authors.lastname: Basher
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Proggananda
authors.initials: P
authors.lastname: Nath
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Prakash
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Faria
authors.initials: F
authors.lastname: Hossain
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh.
authors.forename: Shakhawat
authors.initials: S
authors.lastname: Hossain
authors.affiliation: International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh din63d@icddrb.org.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Mondal
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage / Q000627:therapeutic use; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000627:therapeutic use; D001459:Bangladesh / Q000453:epidemiology; D001707:Biopsy, Needle; D002648:Child; D005260:Female; D006801:Humans; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology / Q000473:pathology; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012008:Recurrence; D012680:Sensitivity and Specificity; D013154:Spleen / Q000469:parasitology*
chemical_list: D000981:Antiprotozoal Agents; C068538:liposomal amphotericin B; D000666:Amphotericin B