title: Assessing the performance of novel software Strain Solution on automated discrimination of Escherichia coli serotypes and their mixtures using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
abstract: O157, O26, and O111 are the most important O serogroups of enterohemorrhagic Escherichia coli worldwide. Recently we reported a strategy for discriminating these serotypes from the others using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) based on the S10-spc-alpha operon gene-encoded ribosomal protein mass spectrum (S10-GERMS) method. To realize the fully automated identification of microorganisms at species- or serotype-level with the concept of S10-GERMS method, novel software named Strain Solution for MALDI-TOF MS was developed. In this study, the Strain Solution was evaluated with a total of 45 E. coli isolates including O26, O91, O103, O111, O115, O121, O128, O145, O157, O159, and untyped serotypes. The Strain Solution could accurately discriminate 92% (11/12) of O157 strains, 100% (13/13) of O26 and O111 strains from the others with three biomarkers in an automated manner. In addition, this software could identify 2 different E. coli strains (K-12 as a non-O157 representative and O157) in mixed samples. The results suggest that Strain Solution will be useful for species- or serotype-level classification of microorganisms in the fields of food safety and diagnostics.
authors.affiliation: Knowledge Hub Aichi, Aichi Science and Technology Foundation, Yakusa, Toyota, Aichi 470-0356, Japan. Electronic address: teruyo.ojima@gmail.com.
authors.forename: Teruyo
authors.initials: T
authors.lastname: Ojima-Kato
authors.affiliation: School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-0073, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Yamamoto
authors.affiliation: Kobe Institute of Health, Minatojimanakamachi, Chuo-ku, Kobe, Hyogo 650-0046, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Iijima
authors.affiliation: Knowledge Hub Aichi, Aichi Science and Technology Foundation, Yakusa, Toyota, Aichi 470-0356, Japan; School of Agriculture, Meijo University, Shiogamaguchi, Tenpaku-ku, Nagoya, Aichi 468-0073, Japan. Electronic address: hiroto@meijo-u.ac.jp.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tamura
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D054324:Enterohemorrhagic Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins
keywords: Escherichia coli; MALDI-TOF MS; S10-GERMS; Strain Solution