title: Clinical significance of TP53 (R72P) and MDM2 (T309G) polymorphisms in breast cancer patients.
abstract: BACKGROUND
  TP53 gene is the most frequently altered tumor suppressor gene in breast cancer. It has been observed that MDM2 plays a central role in regulating the TP53 pathway. This study aimed to investigate the role of TP53 Arg72Pro and MDM2 T309G polymorphisms in breast cancer patients.
  
  
  METHODS
  The TP53 (Arg72Pro) and MDM2 (T309G) polymorphisms were studied in a hospital-based case control study by AS-PCR in 100 breast cancer patients and 100 healthy control subjects.
  
  
  RESULTS
  It was observed that TP53 Arg72Pro polymorphism was significantly associated with breast cancer (χ (2) = 9.92, p = 0.007). A significantly increased breast cancer risk was associated with the Proline allele [odds ratio 1.84 (95 % CI: 1.22-2.77), risk ratio 1.34 (95 % CI: 1.11-1.63), p value 0.003], HER2/neu status (p = 0.01) and distant metastasis (p = 0.05). On the other hand, we have found a significant correlation between MDM2 (T309G) polymorphism with HER2/neu status (χ (2) = 11.14, p = 0.003) and distant metastasis (p value = 0.04).
  
  
  CONCLUSIONS
  Our finding suggests that TP53 (Arg72Pro) polymorphism may play a significant role as risk factor for breast cancer in north Indian breast cancer patients. While MDM2 (T309G) polymorphism may not be directly associated with the risk of breast cancer occurrence in the same population, but it may play role in disease progression by triggering TP53.
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: P
authors.initials: P
authors.lastname: Yadav
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: M
authors.initials: M
authors.lastname: Masroor
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: K
authors.initials: K
authors.lastname: Tanwer
authors.affiliation: Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia.
authors.forename: R
authors.initials: R
authors.lastname: Mir
authors.affiliation: Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, 71491, Saudi Arabia.
authors.forename: J
authors.initials: J
authors.lastname: Javid
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: I
authors.initials: I
authors.lastname: Ahmad
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: M
authors.initials: M
authors.lastname: Zuberi
authors.affiliation: Department of Surgery, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: R C M
authors.initials: RC
authors.lastname: Kaza
authors.affiliation: Department of Surgery, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
authors.affiliation: Department of Pathology, Maulana Azad Medical College and Associated Hospitals, New Delhi, India.
authors.forename: N
authors.initials: N
authors.lastname: Khurana
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India.
authors.forename: P C
authors.initials: PC
authors.lastname: Ray
authors.affiliation: Department of Biochemistry, Maulana Azad Medical College and Associated Hospitals, New Delhi, 110002, India. prasant.mamc@hotmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Saxena
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*
chemical_list: C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
keywords: Breast cancer; MDM2; TP53