title: Multilayered dense collagen-silk fibroin hybrid: a platform for mesenchymal stem cell differentiation towards chondrogenic and osteogenic lineages.
abstract: Type I collagen is a major structural and functional protein in connective tissues. However, collagen gels exhibit unstable geometrical properties, arising from extensive cell-mediated contraction. In an effort to stabilize collagen-based hydrogels, plastic compression was used to hybridize dense collagen (DC) with electrospun silk fibroin (SF) mats, generating multilayered DC-SF-DC constructs. Seeded mesenchymal stem cell (MSC)-mediated DC-SF-DC contraction, as well as growth and differentiation under chondrogenic and osteogenic supplements, were compared to those seeded in DC and on SF alone. The incorporation of SF within DC prevented extensive cell-mediated collagen gel contraction. The effect of the multilayered hybrid on MSC remodelling capacity was also evident at the transcription level, where the expression of matrix metalloproteinases and their inhibitor (MMP1, MMP2, MMP3, MMP13 and Timp1) by MSCs within DC-SF-DC were comparable to those on SF and significantly downregulated in comparison to DC, except for Timp1. Chondrogenic supplements stimulated extracellular matrix production within the construct, stabilizing its multilayered structure and promoting MSC chondrogenic differentiation, as indicated by the upregulation of the genes Col2a1 and Agg and the production of collagen type II. In osteogenic medium there was an upregulation in ALP and OP along with the presence of an apatitic phase, indicating MSC osteoblastic differentiation and matrix mineralization. In sum, these results have implications on the modulation of three-dimensional collagen-based gel structural stability and on the stimulation and maintenance of the MSC committed phenotype inherent to the in vitro formation of chondral tissue and bone, as well as on potential multilayered complex tissues. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada.
authors.forename: Chiara E
authors.initials: CE
authors.lastname: Ghezzi
authors.affiliation: Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada.
authors.forename: Benedetto
authors.initials: B
authors.lastname: Marelli
authors.affiliation: Innovhub-Stazioni Sperimentali per l'Industria, Div. Stazione Sperimentale per la Seta, Milan, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Donelli
authors.affiliation: Innovhub-Stazioni Sperimentali per l'Industria, Div. Stazione Sperimentale per la Seta, Milan, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alessandrino
authors.affiliation: Innovhub-Stazioni Sperimentali per l'Industria, Div. Stazione Sperimentale per la Seta, Milan, Italy.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Freddi
authors.affiliation: Department of Mining and Materials Engineering, McGill University, Montreal, Quebec, Canada.
authors.forename: Showan N
authors.initials: SN
authors.lastname: Nazhat
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D003094:Collagen / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010012:Osteogenesis
chemical_list: D000943:Antigens, Differentiation; D003094:Collagen; D005349:Fibroins
keywords: bone; cartilage; dense collagen gels; extracellular matrix (ECM); real architecture for 3D tissue (RAFT™); silk fibroin