title: From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review.
abstract: Feruloyl esterases (FAEs) are a diverse group of hydrolases widely distributed in plants and microorganisms which catalyzes the cleavage and formation of ester bonds between plant cell wall polysaccharides and phenolic acids. FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of substrates for cleaving ester bonds and synthesizing highadded value molecules through esterification and transesterification reactions. During the past two decades extensive studies have been carried out on the production, characterization and classification of FAEs, however only a few reports of suitable High Throughput Screening assays for this kind of enzymes have been reported. This review is focused on a concise but complete revision of classical to High Throughput Screening methods for FAEs, highlighting its advantages and disadvantages, and finally suggesting future perspectives for this important research field.
authors.forename: Lorena
authors.initials: L
authors.lastname: Ramírez-Velasco
authors.forename: Mariana
authors.initials: M
authors.lastname: Armendáriz-Ruiz
authors.forename: Jorge Alberto
authors.initials: JA
authors.lastname: Rodríguez-González
authors.forename: Marcelo
authors.initials: M
authors.lastname: Müller-Santos
authors.forename: Ali
authors.initials: A
authors.lastname: Asaff-Torres
authors.affiliation: Industrial Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.; Camino Arenero 1227 El Bajío del Arenal, Zapopan, Jalisco, Mexico. jcmateos@ciatej.mx.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mateos-Díaz
mesh_terms: D001709:Biotechnology / Q000379:methods; D002265:Carboxylic Ester Hydrolases; D002473:Cell Wall / Q000737:chemistry; D004951:Esterification; D057166:High-Throughput Screening Assays / Q000379:methods* / Q000639:trends; D059828:Plant Cells / Q000648:ultrastructure; D011134:Polysaccharides / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase