title: Penetrable silica microspheres for immobilization of bovine serum albumin and their application to the study of the interaction between imatinib mesylate and protein by frontal affinity chromatography.
abstract: In the current study, novel featured silica, named penetrable silica, simultaneously containing macropores and mesopores, was immobilized with bovine serum albumin (BSA) via Schiff base method. The obtained BSA-SiO2 was employed as the high-performance liquid chromatographic (HPLC) stationary phase. Firstly, D- and L-tryptophan were used as probes to investigate the chiral separation ability of the BSA-SiO2 stationary phase. An excellent enantioseparation factor was obtained up to 4.3 with acceptable stability within at least 1 month. Next, the BSA-SiO2 stationary phase was applied to study the interaction between imatinib mesylate (IM) and BSA by frontal affinity chromatography. A single type of binding site was found for IM with the immobilized BSA, and the hydrogen-bonding and van der Waals interactions were expected to be contributing interactions based on the thermodynamic studies, and this was a spontaneous process. Compared to the traditional silica for HPLC stationary phase, the proposed penetrable silica microsphere possessed a larger capacity to bond more BSA, minimizing column overloading effects and enhancing enantioseparation ability. In addition, the lower running column back pressure and fast mass transfer were meaningful for the column stability and lifetime. It was a good substrate to immobilize biomolecules for fast chiral resolution and screening drug-protein interactions.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Ma
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liao
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan, 430072, China. shizg@whu.edu.cn.
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Shi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D000068877:Imatinib Mesylate / Q000737:chemistry*; D008863:Microspheres; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism; D014364:Tryptophan / Q000737:chemistry
chemical_list: D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D000068877:Imatinib Mesylate; D014364:Tryptophan
keywords: Bovine serum albumin; Chiral analysis; Frontal affinity chromatography; Imatinib mesylate; Penetrable silica microspheres