title: Synthesis of vanillic acid using whole cell nitrilase of wild and mutant Gordonia terrae.
abstract: The resting cells of Gordonia terrae mutant E9 having enhanced nitrilase activity were used for biotransformation of 4-hydroxy-3-methoxybenzonitrile into vanillic acid. The maximum conversion was observed in 0.1 M phosphate buffer (pH 8.0), using 60 mM substrate and 0.75 mgDCW resting cells in 1 mL reaction at 40 °C. Km of the whole cell nitrilase of wild and mutant strains of G. terrae for this substrate were 20 and 16.6 mM, and Vmax were 0.19 and 0.95 Umg(-1)(DCW), respectively. Fed batch reaction for transformation of 4-hydroxy-3-methoxybenzonitrile using whole cell nitrilase of wild G. terrae resulted in 2.36 g of vanillic acid in 5 h with a catalytic and volumetric productivity of 0.78 gg(-1)(DCW) h(-1) and 4.72 gL(-1)h(-1), respectively. The whole cell nitrilase of G. terrae mutant E9 resulted in higher catalytic and volumetric productivity, i.e., 1.68 gg(-1)DCW h(-1) and 10 gL(-1)h(-1). A total 5.04 g of vanillic acid with 99% purity were accumulated in 100 mL of reaction after 5 h.
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India. bhallatc@rediffmail.com.
authors.forename: Tek Chand
authors.initials: TC
authors.lastname: Bhalla
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India.
authors.lastname: Prashant
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India.
authors.forename: Nisha
authors.initials: N
authors.lastname: Kumari
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India.
authors.forename: Virender
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Himachal Pradesh University, Summer Hill Shimla, Himachal Pradesh, 171005, India.
authors.lastname: Savitri
mesh_terms: D000619:Aminohydrolases / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D033601:Gordonia Bacterium / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D014641:Vanillic Acid / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D000619:Aminohydrolases; C021867:nitrilase; D014641:Vanillic Acid
keywords: 4-hydroxy-3-methoxybenzonitrile; Mutant; Nitrilase; Vanillic acid