title: [Pretreatment with dexmedetomidine ameliorates renal inflammation and oxidative stress in rats with lipopolysaccharide-induced sepsis and acute kidney injury].
abstract: OBJECTIVE
  To investigate the effects of dexmedetomidine on inflammatory reaction, oxidative stress, and renal pathologies in a rat model of lipopolysaccharide (LPS)-induced sepsis.
  
  
  METHODS
  Thirty-two SD rats were randomly divided into 4 groups, including a sham-operated group, LPS group with LPS (5 mg/kg) injection via the caudal vein 30 min before the operation, dexmedetomidine (Dex) +LPS group with additional Dex (10 µg/kg) injection via the caudal vein 10 min before LPS injection, and yohimbine+DEX+LPS group with intraperitoneal yohimbine (1 mg/kg) injection 40 min before and Dex injection 10 min before LPS injection. The levels of IL-1β, SOD and MDA in the plasma and renal tissues were determined, and the renal pathologies were examined.
  
  
  RESULTS
  Compared with the sham-operated rats, the rats in LPS group showed significantly increased IL-1β and MDA levels and lowered SOD activity in the plasma and renal tissues (P<0.05) with obvious renal pathologies. Dex pretreatment obviously lowered IL-1β and MDA levels and enhanced SOD activity in the plasma and renal tissues in LPS-challenged rats (P<0.05), and significantly lessened LPS-induced renal pathologies.
  
  
  CONCLUSIONS
  Dex can protect the rats against LPS-induced renal injury by alleviating the inflammatory reactions and cytokine oxidative stress, and this effect is mediated possibly by α2 receptors.
authors.affiliation: Department of Intensive Care Unit, Guangzhou Economic Technological Development Zone Hospital, Guangzhou 510730, China. E-mail: 13527896098@139.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Chen
authors.forename: Chu-Lian
authors.initials: CL
authors.lastname: Gong
authors.forename: Fang
authors.initials: F
authors.lastname: Tan
authors.forename: Shao-Li
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy*; D000818:Animals; D020927:Dexmedetomidine / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology; D008070:Lipopolysaccharides; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000139:chemically induced / Q000188:drug therapy*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D008315:Malondialdehyde; D020927:Dexmedetomidine; D013482:Superoxide Dismutase