title: One-pot synthesis of active copper-containing carbon dots with laccase-like activities.
abstract: Herein, an effective strategy for designing a new type of nanozyme, blue fluorescent laccase mimics, is reported. Active copper-containing carbon dots (Cu-CDs) were synthesized through a simple, nontoxic and one-pot hydrothermal method, which showed favorable photoluminescence properties and good photostability under high-salt conditions or in a broad pH range (3.0-13.5). The Cu-CDs possessed intrinsic laccase-like activities and could catalyze the oxidation of the laccase substrate p-phenylenediamine (PPD) to produce a typical color change from colorless to brown. Poly(methacrylic acid sodium salt) (PMAA) not only was used as the carbon source and reducing agent, but also provided carboxyl groups to assist flocculation between Cu-CDs and polyacrylamide, which facilitated the removal of PPD. Importantly, the intrinsic fluorescence of the as-prepared Cu-CDs could indicate the presence of hydroquinone, one of the substrates of laccases, based on laccase mimics and fluorescence quenching.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology & Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. mengxw@mail.ipc.ac.cn tangfq@mail.ipc.ac.cn.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology & Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. mengxw@mail.ipc.ac.cn tangfq@mail.ipc.ac.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology & Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. mengxw@mail.ipc.ac.cn tangfq@mail.ipc.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology & Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. mengxw@mail.ipc.ac.cn tangfq@mail.ipc.ac.cn.
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology & Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. mengxw@mail.ipc.ac.cn tangfq@mail.ipc.ac.cn.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
mesh_terms: D002244:Carbon / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry*
chemical_list: D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D002244:Carbon; D003300:Copper; D042845:Laccase