title: [The severity of gestational diabetes mellitus affects microvascular dysfunction measured three years after pregnancy that may be related to increased oxidative stress].
abstract: BACKGROUND
  Oxidative-nitrative stress and poly(ADP-ribose) polymerase activation observed in gestational diabetes may play role in the increased cardiovascular risk in later life.
  
  
  OBJECTIVE
  The present study aimed to examine the influence of the severity of previous gestational diabetes (insulin need) on vascular function three years after delivery. Furthermore, the authors investigated the relation of vascular function with oxidative-nitrative stress and poly(ADP-ribose) polymerase activation.
  
  
  METHODS
  Macrovascular function was measured by applanation tonometry; microvascular reactivity was assessed by provocation tests during Laser-Doppler flowmetry in 40 women who had gestational diabetes 3 years before the study. Oxidative-nitrative stress and poly(ADP-ribose) polymerase activity in blood components were determined by colorimetry and immunohistochemistry.
  
  
  RESULTS
  Three years after insulin treated gestational diabetes impaired microvascular function and increased oxidative stress was observed compared to mild cases.
  
  
  CONCLUSIONS
  The severity of previous gestational diabetes affects microvascular dysfunction that is accompanied by elevated oxidative stress. Nitrative stress and poly(ADP-ribose) polymerase activity correlates with certain vascular factors not related to the severity of the disease.
authors.affiliation: Élettani Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Tűzoltó u. 37-47., 1094.|Klinikai Kísérleti Kutató- és Humán Élettani Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Eszter Mária
authors.initials: EM
authors.lastname: Horváth
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Rita
authors.initials: R
authors.lastname: Mágenheim
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Beatrix Annamária
authors.initials: BA
authors.lastname: Domján
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Viktória
authors.initials: V
authors.lastname: Ferencz
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Tímea
authors.initials: T
authors.lastname: Tänczer
authors.affiliation: Szent Imre Egyetemi Oktatókórház Budapest.
authors.forename: Eszter
authors.initials: E
authors.lastname: Szabó
authors.affiliation: Élettani Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest, Tűzoltó u. 37-47., 1094.|Klinikai Kísérleti Kutató- és Humán Élettani Intézet, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Rita
authors.initials: R
authors.lastname: Benkő
authors.affiliation: Department of Anesthesiology, University of Texas Medical Branch Galveston, TX, USA.
authors.forename: Csaba
authors.initials: C
authors.lastname: Szabó
authors.affiliation: I. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.|Research Department of Epidemiology and Public Health, University College London London, Egyesült Királyság.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Tabák
authors.affiliation: II. Belgyógyászati Klinika, Semmelweis Egyetem, Általános Orvostudományi Kar Budapest.
authors.forename: Anikó
authors.initials: A
authors.lastname: Somogyi
mesh_terms: D000328:Adult; D002318:Cardiovascular Diseases / Q000378:metabolism* / Q000503:physiopathology; D016640:Diabetes, Gestational / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology*; D004789:Enzyme Activation; D005260:Female; D005500:Follow-Up Studies; D005609:Free Radicals / Q000378:metabolism*; D006801:Humans; D008833:Microcirculation; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D012720:Severity of Illness Index
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases
keywords: follow-up; gestational diabetes; gestatiós diabetes mellitus; oxidative stress; oxidatív stressz; utánkövetés; vascular function; vascularis funkció