title: Assessment of microbial populations within Chicago area nearshore waters and interfaces with river systems.
abstract: The Chicago area locks separate and control water flow between the freshwaters of Lake Michigan and the network of Illinois waterways. Under extreme storm conditions, however, the locks are opened and storm waters, untreated waste, and runoff are released directly into the lake. These combined sewer overflow (CSO) events introduce microbes, viruses, and nutrients such as nitrogen and phosphorous into nearshore waters which likely affect the native species. We collected surface water samples from four Chicago area beaches - Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach - every two weeks from May 13 through August 5, 2014. Sampling was conducted with four biological replicates for each sampling date and location, resulting in 112 samples. Each community was surveyed through targeted sequencing of the V4 16S rRNA gene. Technical replicates were also sequenced and are included in this dataset. Taxa were identified using Mothur. Raw sequence data is available via NCBI׳s SRA database (part of BioProject PRJNA245802).
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Emily
authors.initials: E
authors.lastname: Sible
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Alexandria
authors.initials: A
authors.lastname: Cooper
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Kema
authors.initials: K
authors.lastname: Malki
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Bruder
authors.affiliation: Bioinformatics Program, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA ; Department of Computer Science, Loyola University Chicago, 820N Michigan Ave, Chicago, IL 60611, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: Hatzopoulos
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA.
authors.forename: Siobhan C
authors.initials: SC
authors.lastname: Watkins
authors.affiliation: Department of Biology, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA ; Bioinformatics Program, Loyola University Chicago, 1032W Sheridan Rd, Chicago, IL 60660, USA ; Department of Computer Science, Loyola University Chicago, 820N Michigan Ave, Chicago, IL 60611, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Putonti
keywords: Freshwater; Lake Michigan; Metagenomics; Microbial community