title: [Establishment of MDCK cell models expressing human MATE1 or co-expressing with human OCT1 or OCT2].
abstract: To establish single- and double-transfected transgenic cells stably expressing hMATE1, hMATE1 cDNA was cloned by RT-PCR from human cryopreserved kidney tissue, and subcloned into pcDNA3.1(+) plasmid by virtue of both HindIII and Kpn I restriction enzyme sites. Subsequently, the recombined pcDNA3.1(+)- hMATE1 plasmid was transfected into MDCK, MDCK-hOCT1 or MDCK-hOCT2 cells using Lipofectamine 2000 Reagent. After a 14-day-cultivation with hygromycin B at the concentration of 400 µg · mL(-1), all clones were screened with DAPI and MPP+ as substrates to identify the best candidate. The mRNA content of hMATE1, the cellular accumulation of metformin with or without cimetidine as inhibitor, or transportation of cimetidine was further valuated. The results showed that all of the three cell models over expressed hMATE1 mRNA. The cellular accumulation of metformin in MDCK-hMATE1 was 17.6 folds of the control cell, which was significantly inhibited by 100 µmol · L(-1) cimetidine. The transcellular transport parameter net efflux ratios of cimetidine across MDCK-hOCT1/hMATE1 and MDCK-hOCT2/hMATE1 monolayer were 17.5 and 3.65, respectively. In conclusion, cell models with good hMATE1 function have been established successfully, which can be applied to study the drug transport or drug-drug interaction involving hMATE1 alone or together with hOCT1/2 in vitro.
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Lei
authors.forename: Si-yuan
authors.initials: SY
authors.lastname: Sun
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Li
authors.forename: Mei-juan
authors.initials: MJ
authors.lastname: Tu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
authors.forename: Hui-di
authors.initials: HD
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001692:Biological Transport; D002927:Cimetidine / Q000494:pharmacology; D018076:DNA, Complementary; D004285:Dogs; D004347:Drug Interactions; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D008687:Metformin / Q000494:pharmacology; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D027701:Organic Cation Transport Proteins; C521869:SLC47A1 protein, human; D002927:Cimetidine; D008687:Metformin