title: Ruthenium Complex "Light Switches" that are Selective for Different G-Quadruplex Structures.
abstract: Recognition and regulation of G-quadruplex nucleic acid structures is an important goal for the development of chemical tools and medicinal agents. The addition of a bromo-substituent to the dipyridylphenazine (dppz) ligands in the photophysical "light switch", [Ru(bpy)2 dppz](2+) , and the photochemical "light switch", [Ru(bpy)2 dmdppz](2+) , creates compounds with increased selectivity for an intermolecular parallel G-quadruplex and the mixed-hybrid G-quadruplex, respectively. When [Ru(bpy)2 dppz-Br](2+) and [Ru(bpy)2 dmdppz-Br](2+) are incubated with the G-quadruplexes, they have a stabilizing effect on the DNA structures. Activation of [Ru(bpy)2 dmdppz-Br](2+) with light results in covalent adduct formation with the DNA. These complexes demonstrate that subtle chemical modifications of Ru(II) complexes can alter G-quadruplex selectivity, and could be useful for the rational design of in vivo G-quadruplex probes.
authors.affiliation: Department of Chemistry, University of Kentucky, 505 Rose Street, Lexington, KY 40506 (USA).
authors.forename: Erin
authors.initials: E
authors.lastname: Wachter
authors.affiliation: Department of Chemistry, University of Kentucky, 505 Rose Street, Lexington, KY 40506 (USA).
authors.forename: Diego
authors.initials: D
authors.lastname: Moyá
authors.affiliation: Department of Chemistry, University of Kentucky, 505 Rose Street, Lexington, KY 40506 (USA).
authors.forename: Sean
authors.initials: S
authors.lastname: Parkin
authors.affiliation: Department of Chemistry, University of Kentucky, 505 Rose Street, Lexington, KY 40506 (USA). ec.glazer@uky.edu.
authors.forename: Edith C
authors.initials: EC
authors.lastname: Glazer
mesh_terms: D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D009696:Nucleic Acids / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D009696:Nucleic Acids; D010619:Phenazines; D012428:Ruthenium; D004247:DNA
keywords: G-quadruplexes; light switch; photochemistry; photophysics; ruthenium