title: Comparison of antioxidant activity of the fruits derived from in vitro propagated and traditionally cultivated tayberry plants.
abstract: BACKGROUND
  Tayberry is a hybrid between Rubus fruticosus L. and Rubus idaeus L. These fruits contain valuable vitamins and antioxidants. An effective protocol for micropropagation of tayberry plants is here described. Different concentrations of cytokinins (6-benzylaminopurine, zeatin, and 6-(γ,γ-dimethylallylamino)purine) were added in Murashige and Skoog, 1962 (MS) medium to micropropagation using stem tip and nodal explants.
  
  
  RESULTS
  The highest propagation rate was recorded on MS medium containing 2 mg L(-1) zeatin, where the shoot formation resulted in 3.4 shoots per stem tip explant after 4 weeks of culture. It was found that half-strength MS medium with 0.1 mg L(-1) indole-3-butyric acid was the best for plant rooting. For ex vitro acclimatization of plants, the mixture of peat, soil, and perlite (1:1:1 v/v/v) was the most suitable planting substrate for hardening. The micropropagation protocol described in this study might be useful for the production of healthy plant materials. Tayberry fruits from in vitro propagated plants and adapted to the field conditions possessed higher antioxidant capacity in comparison to traditionally cultivated plants.
  
  
  CONCLUSIONS
  Fruit extracts of micropropagated tayberry plants and adapted to field conditions can be used as a rich source of natural antioxidants. © 2015 Society of Chemical Industry.
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria.
authors.forename: Ely G
authors.initials: EG
authors.lastname: Zayova
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria.
authors.forename: Ira V
authors.initials: IV
authors.lastname: Stancheva
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria.
authors.forename: Maria P
authors.initials: MP
authors.lastname: Geneva
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Petrova
authors.affiliation: Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria.
authors.forename: Ludmila I
authors.initials: LI
authors.lastname: Dimitrova
mesh_terms: D000383:Agriculture; D000975:Antioxidants / Q000378:metabolism*; D003470:Culture Media; D003583:Cytokinins / Q000378:metabolism; D005638:Fruit / Q000254:growth & development / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D065856:Rubus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D003583:Cytokinins; D007211:Indoles; C014612:indolebutyric acid
keywords: antioxidant capacity; flavonoids; in vitro shoot induction; phenols; tayberry