title: Articular cartilage repair with recombinant human type II collagen/polylactide scaffold in a preliminary porcine study.
abstract: The purpose of this study was to investigate the potential of a novel recombinant human type II collagen/polylactide scaffold (rhCo-PLA) in the repair of full-thickness cartilage lesions with autologous chondrocyte implantation technique (ACI). The forming repair tissue was compared to spontaneous healing (spontaneous) and repair with a commercial porcine type I/III collagen membrane (pCo). Domestic pigs (4-month-old, n = 20) were randomized into three study groups and a circular full-thickness chondral lesion with a diameter of 8 mm was created in the right medial femoral condyle. After 3 weeks, the chondral lesions were repaired with either rhCo-PLA or pCo together with autologous chondrocytes, or the lesion was only debrided and left untreated for spontaneous repair. The repair tissue was evaluated 4 months after the second operation. Hyaline cartilage formed most frequently in the rhCo-PLA treatment group. Biomechanically, there was a trend that both treatment groups resulted in better repair tissue than spontaneous healing. Adverse subchondral bone reactions developed less frequently in the spontaneous group (40%) and the rhCo-PLA treated group (50%) than in the pCo control group (100%). However, no statistically significant differences were found between the groups. The novel rhCo-PLA biomaterial showed promising results in this proof-of-concept study, but further studies will be needed in order to determine its effectiveness in articular cartilage repair. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:745-753, 2016.
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.
authors.forename: Virpi
authors.initials: V
authors.lastname: Muhonen
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.
authors.forename: Eve
authors.initials: E
authors.lastname: Salonius
authors.affiliation: Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Haaparanta
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.
authors.forename: Elina
authors.initials: E
authors.lastname: Järvinen
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.|Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland.
authors.forename: Teemu
authors.initials: T
authors.lastname: Paatela
authors.affiliation: Laboratory Animal Center, University of Helsinki, Helsinki, Finland.
authors.forename: Anna
authors.initials: A
authors.lastname: Meller
authors.affiliation: Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Hannula
authors.affiliation: Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.
authors.forename: Mimmi
authors.initials: M
authors.lastname: Björkman
authors.affiliation: Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland.
authors.forename: Tuomo
authors.initials: T
authors.lastname: Pyhältö
authors.affiliation: Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Ellä
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.|Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland.
authors.forename: Anna
authors.initials: A
authors.lastname: Vasara
authors.affiliation: Department of Applied Physics, University of Eastern Finland, Kuopio, Finland.|Department of Clinical Neurophysiology, Kuopio University Hospital, Kuopio, Finland.
authors.forename: Juha
authors.initials: J
authors.lastname: Töyräs
authors.affiliation: Department of Electronics and Communications Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Kellomäki
authors.affiliation: Department of Orthopaedics and Traumatology, University of Helsinki, Helsinki, Finland.|Department of Orthopaedics and Traumatology, Helsinki University Hospital, Helsinki, Finland.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Kiviranta
mesh_terms: D000818:Animals; D002357:Cartilage Diseases / Q000628:therapy*; D002358:Cartilage, Articular / Q000293:injuries*; D019902:Chondrocytes / Q000637:transplantation*; D024043:Collagen Type II; D005260:Female; D020342:Finite Element Analysis; D006801:Humans; D011091:Polyesters; D011897:Random Allocation; D013552:Swine; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D024043:Collagen Type II; D011091:Polyesters; C033616:poly(lactide)
keywords: ACI; cartilage repair; polylactide; recombinant type II collagen; scaffold