title: Microbial "social networks".
abstract: BACKGROUND
  It is well understood that distinct communities of bacteria are present at different sites of the body, and that changes in the structure of these communities have strong implications for human health. Yet, challenges remain in understanding the complex interconnections between the bacterial taxa within these microbial communities and how they change during the progression of diseases. Many recent studies attempt to analyze the human microbiome using traditional ecological measures and cataloging differences in bacterial community membership. In this paper, we show how to push metagenomic analyses beyond mundane questions related to the bacterial taxonomic profiles that differentiate one sample from another.
  
  
  METHODS
  We develop tools and techniques that help us to investigate the nature of social interactions in microbial communities, and demonstrate ways of compactly capturing extensive information about these networks and visually conveying them in an effective manner. We define the concept of bacterial "social clubs", which are groups of taxa that tend to appear together in many samples. More importantly, we define the concept of "rival clubs", entire groups that tend to avoid occurring together in many samples. We show how to efficiently compute social clubs and rival clubs and demonstrate their utility with the help of examples including a smokers' dataset and a dataset from the Human Microbiome Project (HMP).
  
  
  RESULTS
  The tools developed provide a framework for analyzing relationships between bacterial taxa modeled as bacterial co-occurrence networks. The computational techniques also provide a framework for identifying clubs and rival clubs and for studying differences in the microbiomes (and their interactions) of two or more collections of samples.
  
  
  CONCLUSIONS
  Microbial relationships are similar to those found in social networks. In this work, we assume that strong (positive or negative) tendencies to co-occur or co-infect is likely to have biological, physiological, or ecological significance, possibly as a result of cooperation or competition. As a consequence of the analysis, a variety of biological interpretations are conjectured. In the human microbiome context, the pattern of strength of interactions between bacterial taxa is unique to body site.
authors.forename: Mitch
authors.initials: M
authors.lastname: Fernandez
authors.forename: Juan D
authors.initials: JD
authors.lastname: Riveros
authors.forename: Michael
authors.initials: M
authors.lastname: Campos
authors.forename: Kalai
authors.initials: K
authors.lastname: Mathee
authors.forename: Giri
authors.initials: G
authors.lastname: Narasimhan
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D005260:Female; D006801:Humans; D008297:Male; D056186:Metagenomics / Q000379:methods*; D064307:Microbiota; D008875:Middle Aged; D012907:Smoking