title: Photocatalytical Antibacterial Activity of Mixed-Phase TiO2 Nanocomposite Thin Films against Aggregatibacter actinomycetemcomitans.
abstract: Mixed-phase TiO2 nanocomposite thin films consisting of anatase and rutile prepared on commercially pure Ti sheets via the electrochemical anodization and annealing treatments were investigated in terms of their photocatalytic activity for antibacterial use around dental implants. The resulting films were characterized by scanning electron microscopy (SEM), and X-ray diffraction (XRD). The topology was assessed by White Light Optical Profiling (WLOP) in the Vertical Scanning Interferometer (VSI) mode. Representative height descriptive parameters of roughness R a and R z were calculated. The photocatalytic activity of the resulting TiO2 films was evaluated by the photodegradation of Rhodamine B (RhB) dye solution. The antibacterial ability of the photocatalyst was examined by Aggregatibacter actinomycetemcomitans suspensions in a colony-forming assay. XRD showed that anatase/rutile mixed-phase TiO2 thin films were predominantly in anatase and rutile that were 54.6 wt% and 41.9 wt%, respectively. Craters (2-5 µm) and protruding hills (10-50 µm) on Ti substrates were produced after electrochemical anodization with higher R a and R z surface roughness values. Anatase/rutile mixed-phase TiO2 thin films showed 26% photocatalytic decolorization toward RhB dye solution. The number of colonizing bacteria on anatase/rutile mixed-phase TiO2 thin films was decreased significantly in vitro. The photocatalyst was effective against A. actinomycetemcomitans colonization.
authors.affiliation: Department of Oral Implantology, Faculty of Dentistry, Istanbul University, 34093 Istanbul, Turkey.
authors.forename: Sinem
authors.initials: S
authors.lastname: Yeniyol
authors.affiliation: Metallurgical and Materials Engineering Department, Faculty of Engineering, Istanbul University, Avcilar, 34320 Istanbul, Turkey.
authors.forename: Ilven
authors.initials: I
authors.lastname: Mutlu
authors.affiliation: Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Mechanical Engineering, Istanbul Aydın University, 34668 Istanbul, Turkey.
authors.forename: Behiye
authors.initials: B
authors.lastname: Yüksel
authors.affiliation: Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: Robert Joseph
authors.initials: RJ
authors.lastname: Boylan
authors.affiliation: Department of Metallurgical and Materials Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, 34469 Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ürgen
authors.affiliation: Department of Oral Implantology, Faculty of Dentistry, Istanbul University, 34093 Istanbul, Turkey.
authors.forename: Zihni Cüneyt
authors.initials: ZC
authors.lastname: Karabuda
authors.affiliation: Department of Oral Implantology, Faculty of Dentistry, Istanbul University, 34093 Istanbul, Turkey.
authors.forename: Cansu
authors.initials: C
authors.lastname: Basegmez
authors.affiliation: Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, NY 10010, USA.
authors.forename: John Lawrence
authors.initials: JL
authors.lastname: Ricci
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects* / Q000502:physiology* / Q000528:radiation effects; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000138:chemical synthesis / Q000528:radiation effects; D002384:Catalysis / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects; D008027:Light; D053761:Nanocomposites / Q000008:administration & dosage* / Q000737:chemistry* / Q000528:radiation effects; D044367:Phase Transition; D014025:Titanium / Q000008:administration & dosage* / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D000900:Anti-Bacterial Agents; C009495:titanium dioxide; D014025:Titanium