title: Early Detection of Junctional Adhesion Molecule-1 (JAM-1) in the Circulation after Experimental and Clinical Polytrauma.
abstract: Severe tissue trauma-induced systemic inflammation is often accompanied by evident or occult blood-organ barrier dysfunctions, frequently leading to multiple organ dysfunction. However, it is unknown whether specific barrier molecules are shed into the circulation early after trauma as potential indicators of an initial barrier dysfunction. The release of the barrier molecule junctional adhesion molecule-1 (JAM-1) was investigated in plasma of C57BL/6 mice 2 h after experimental mono- and polytrauma as well as in polytrauma patients (ISS ≥ 18) during a 10-day period. Correlation analyses were performed to indicate a linkage between JAM-1 plasma concentrations and organ failure. JAM-1 was systemically detected after experimental trauma in mice with blunt chest trauma as a driving force. Accordingly, JAM-1 was reduced in lung tissue after pulmonary contusion and JAM-1 plasma levels significantly correlated with increased protein levels in the bronchoalveolar lavage as a sign for alveolocapillary barrier dysfunction. Furthermore, JAM-1 was markedly released into the plasma of polytrauma patients as early as 4 h after the trauma insult and significantly correlated with severity of disease and organ dysfunction (APACHE II and SOFA score). The data support an early injury- and time-dependent appearance of the barrier molecule JAM-1 in the circulation indicative of a commencing trauma-induced barrier dysfunction.
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Denk
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wiegner
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Felix M
authors.initials: FM
authors.lastname: Hönes
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: David A C
authors.initials: DA
authors.lastname: Messerer
authors.affiliation: Institute of Pathophysiology and Process Development in Anesthesia, University of Ulm, 89081 Ulm, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Radermacher
authors.affiliation: Department of Anesthesiology, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Weiss
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Kalbitz
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Ehrnthaller
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Braumüller
authors.affiliation: Institute of Pathophysiology and Process Development in Anesthesia, University of Ulm, 89081 Ulm, Germany.
authors.forename: Oscar
authors.initials: O
authors.lastname: McCook
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Gebhard
authors.affiliation: Department of Orthopedic Surgery, University of Ulm, RKU, 89081 Ulm, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Weckbach
authors.affiliation: Department of Orthopedic Trauma, Hand, Plastic and Reconstructive Surgery, University Hospital of Ulm, 89081 Ulm, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Huber-Lang
mesh_terms: D018806:APACHE; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000097:blood*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009104:Multiple Trauma / Q000097:blood*; D011956:Receptors, Cell Surface / Q000097:blood*; D012720:Severity of Illness Index
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; C498686:F11r protein, mouse; D011956:Receptors, Cell Surface