title: Cell-Penetrating Peptide as a Means of Directing the Differentiation of Induced-Pluripotent Stem Cells.
abstract: Protein transduction using cell-penetrating peptides (CPPs) is useful for the delivery of large protein molecules, including some transcription factors. This method is safer than gene transfection methods with a viral vector because there is no risk of genomic integration of the exogenous DNA. Recently, this method was reported as a means for the induction of induced pluripotent stem (iPS) cells, directing the differentiation into specific cell types and supporting gene editing/correction. Furthermore, we developed a direct differentiation method to obtain a pancreatic lineage from mouse and human pluripotent stem cells via the protein transduction of three transcription factors, Pdx1, NeuroD, and MafA. Here, we discuss the possibility of using CPPs as a means of directing the differentiation of iPS cells and other stem cell technologies.
authors.affiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjyo, Kumamoto 860-8556, Japan. kaitsuka@kumamoto-u.ac.jp.
authors.forename: Taku
authors.initials: T
authors.lastname: Kaitsuka
authors.affiliation: Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjyo, Kumamoto 860-8556, Japan. tomikt@kumamoto-u.ac.jp.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tomizawa
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D057846:Cell-Penetrating Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D065150:Cellular Reprogramming / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology; D015870:Gene Expression; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D051473:Maf Transcription Factors, Large / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009536:Niacinamide / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D014704:Veratrum Alkaloids / Q000494:pharmacology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D057846:Cell-Penetrating Peptides; D002934:Cinnamates; D052216:Glucagon-Like Peptide 1; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D051473:Maf Transcription Factors, Large; D009419:Nerve Tissue Proteins; D009536:Niacinamide; D015534:Trans-Activators; D014212:Tretinoin; D014704:Veratrum Alkaloids; C542465:3-keto-N-aminoethylaminoethylcaproyldihydrocinnamoyl cyclopamine; C485159:MAFA protein, human; C084010:pancreatic and duodenal homeobox 1 protein; C093564:Neurogenic differentiation factor 1
keywords: cell-penetrating peptide; induced pluripotent stem cell; pancreatic differentiation; poly-arginine; protein transduction