title: RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences.
abstract: The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels.
authors.affiliation: Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands. M.Derksen@ncmls.ru.nl.|Department of Biomolecular Chemistry, Radboud Institute for Molecular Life Sciences, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands. M.Derksen@ncmls.ru.nl.
authors.forename: Merel
authors.initials: M
authors.lastname: Derksen
authors.affiliation: Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands. vicky.mertens@student.ru.nl.
authors.forename: Vicky
authors.initials: V
authors.lastname: Mertens
authors.affiliation: Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands. G.Pruijn@ncmls.ru.nl.|Department of Biomolecular Chemistry, Radboud Institute for Molecular Life Sciences, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands. G.Pruijn@ncmls.ru.nl.
authors.forename: Ger J M
authors.initials: GJ
authors.lastname: Pruijn
mesh_terms: D000818:Animals; D055785:Gene Knockdown Techniques / Q000379:methods; D020868:Gene Silencing; D006801:Humans; D012323:RNA Processing, Post-Transcriptional; D043262:Ribonuclease P / Q000378:metabolism*
chemical_list: D043262:Ribonuclease P
keywords: RNA cleavage; RNA knockdown; RNA targeting; RNase P; external guide sequence