title: Developing Aptamers by Cell-Based SELEX.
abstract: The reliable targeting of cell surface disease-associated proteins is a major challenge in chemical biology and molecular medicine. In this regard, aptamers represent a very attractive and innovative class of ligand molecules. Aptamers are generated by a reiterated in vitro procedure, named SELEX (Systematic Evolution of Ligands by Exponential enrichment). In order to generate aptamers for heavily modified cell surface-bound proteins and transmembrane receptors, the SELEX procedure has been recently adapted to the use of living cells as complex targets (referred as "cell-SELEX"). Here we give an overview on the most recent advances in the field of cell-SELEX technology, providing a detailed description of the differential cell-SELEX approach that has been developed in our laboratory to identify specific signatures for human malignant glioma and non-small-cell lung cancer. The procedures used for the evaluation of binding specificity and for the preliminary identification of potential target receptors will be also described.
authors.affiliation: Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR "G. Salvatore", Via S. Pansini 5, 80131, Naples, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Catuogno
authors.affiliation: Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR "G. Salvatore", Via S. Pansini 5, 80131, Naples, Italy.
authors.forename: Carla Lucia
authors.initials: CL
authors.lastname: Esposito
authors.affiliation: Istituto per l'Endocrinologia e l'Oncologia Sperimentale del CNR "G. Salvatore", Via S. Pansini 5, 80131, Naples, Italy. defranci@unina.it.
authors.forename: Vittorio
authors.initials: V
authors.lastname: de Franciscis
mesh_terms: D052157:Aptamers, Nucleotide; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D015723:Gene Library; D005910:Glioma / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D011485:Protein Binding; D052156:SELEX Aptamer Technique; D017422:Sequence Analysis, DNA
chemical_list: D052157:Aptamers, Nucleotide; D008565:Membrane Proteins
keywords: Aptamer; Cell-SELEX; Glioma; NSCLC; RTK