title: Short Duration Combined Mild Hypothermia Improves Resuscitation Outcomes in a Porcine Model of Prolonged Cardiac Arrest.
abstract: OBJECTIVE
  In this study, our aim was to investigate the effects of combined hypothermia with short duration maintenance on the resuscitation outcomes in a porcine model of ventricular fibrillation (VF).
  
  
  METHODS
  Fourteen porcine models were electrically induced with VF and untreated for 11 mins. All animals were successfully resuscitated manually and then randomized into two groups: combined mild hypothermia (CH group) and normothermia group (NT group). A combined hypothermia of ice cold saline infusion and surface cooling was implemented in the animals of the CH group and maintained for 4 hours. The survival outcomes and neurological function were evaluated every 24 hours until a maximum of 96 hours. Neuron apoptosis in hippocampus was analyzed.
  
  
  RESULTS
  There were no significant differences in baseline physiologies and primary resuscitation outcomes between both groups. Obvious improvements of cardiac output were observed in the CH group at 120, 180, and 240 mins following resuscitation. The animals demonstrated better survival at 96 hours in the CH group when compared to the NT group. In comparison with the NT group, favorable neurological functions were observed in the CH group.
  
  
  CONCLUSIONS
  Short duration combined cooling initiated after resuscitation improves survival and neurological outcomes in a porcine model of prolonged VF.
authors.affiliation: Emergency Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China ; Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: Emergency Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China ; Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Zhengfei
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Emergency Department, Affiliated Dongguan Hospital, Medical College of Jinan University, Dongguan 523900, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.affiliation: Biomedical Engineering Department, Guangzhou Medical University, Guangzhou 511436, China.
authors.forename: Youde
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Emergency Department, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China ; Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou 510120, China.
authors.forename: Zitong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou 510120, China ; School of Biomedical Engineering, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D016887:Cardiopulmonary Resuscitation / Q000379:methods* / Q000706:statistics & numerical data*; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000628:therapy*; D007036:Hypothermia, Induced / Q000379:methods*; D008297:Male; D013552:Swine; D013997:Time Factors; D016896:Treatment Outcome