title: Expansion of CD14(+)CD16(+) monocytes is related to acute leukemia.
abstract: BACKGROUND
  
  
  
  OBJECTIVE
  Aim to investigate the proportion of CD14(+)CD16(+) monocytes and understand the pathogenesis of this monocyte subset in acute leukemia.
  
  
  METHODS
  Flow cytometry was utilized to study the phenotype expression of CD14(+)CD16(+) monocytes and CD3(+) T lymphocytes in peripheral blood derived from patients with acute leukemia. All the data were analyzed by SPSS 13.0 software.
  
  
  RESULTS
  The proportion of CD14(+)CD16(+) monocytes including both intermediate and non-classical monocytes, increased significantly in patients with acute leukemia and changed negatively or positively according to the disease process. Meanwhile, the proportion of CD14(+)CD16(+) monocytes was inversely correlated with absolute number of CD4(+) T lymphocytes, ratio of CD4(+)/CD8(+) T cells, and positively correlated with the proportion of neutrophil granulocytes.
  
  
  CONCLUSIONS
  The proportion of CD14(+)CD16(+) monocytes (especially the intermediate subpopulation) is related to the progression of acute leukemia, and the expansion of this monocyte subset could indicate the severity of the disease.
authors.affiliation: Institute of Public Health, Taishan Medical University No.619 Changcheng Road, Taian, Shandong, People's Republic of China.
authors.forename: Xin-Quan
authors.initials: XQ
authors.lastname: Jiang
authors.affiliation: Clinical Laboratory, Maternity and Child Health Care Hospital District of Dong Chang Fu, Liaocheng, Shandong, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory, Taishan Sanatorium Taian, Shandong, People's Republic of China.
authors.forename: Hong-Ai
authors.initials: HA
authors.lastname: Liu
authors.affiliation: Institute of Public Health, Taishan Medical University No.619 Changcheng Road, Taian, Shandong, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yuan
authors.affiliation: Center for Disease Control and Prevention Taian, Shandong, People's Republic of China.
authors.forename: Pei-Qiang
authors.initials: PQ
authors.lastname: Hou
authors.affiliation: Center for Disease Control and Prevention Taian, Shandong, People's Republic of China.
authors.forename: Rong-Qiang
authors.initials: RQ
authors.lastname: Zhang
authors.affiliation: Institute of Public Health, Taishan Medical University No.619 Changcheng Road, Taian, Shandong, People's Republic of China.
authors.forename: Tuo
authors.initials: T
authors.lastname: Wu
keywords: Acute leukemia; T lymphocyte; inflammation; monocyte subset