title: Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
abstract: BACKGROUND
  Resistin (Retn) is a cytokine which has a controversial physiological role regarding its involvement with obesity and type II diabetes mellitus. Recently, murine Retn was found to be a possibly potential regulator of hematopoiesis in mice shown in the screening results of a set of gene chips which mapped the expression level of murine genes during regeneration of impaired bone marrow (BM) by 5-fluorouracil.
  
  
  RESULTS
  Recombinant mice Retn was expressed in Escherichia coli and purified using ion exchange chromatography. Totally 11.4 mg rmRetn was obtained from 500 ml culture with endotoxin level less than 1.0 EU/ug. The purity of recombinant murine Resistin reached to at least 97.6% via SDS-PAGE analysis and HPLC. The protein possessed chemotaxis effects in the mouse aortic endothelial cells in vitro in transwell analysis. In vitro, rmRetn could up regulate the CFU number of mice BM and after rmRetn was administered, the cell number of murine bone marrow was significantly increased in vivo after chemotherapy. Finally, rmRetn was found able to protect mice from the chemotoxicity of 5-fluorouracil.
  
  
  CONCLUSIONS
  The discovery demonstrated a new function of murine Retn and suggested that it could potentially accelerate bone marrow regeneration post chemotherapy.
authors.affiliation: The Center of Research Laboratory, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, 200030, China. sunflowerwang@hotmail.com.|Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, 200080, China. sunflowerwang@hotmail.com.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China. g_jin@sjtu.edu.cn.|College of Pharmacy, Washington State University, Spokane, WA, 99202, USA. g_jin@sjtu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Gao
authors.affiliation: College of Pharmacy, Washington State University, Spokane, WA, 99202, USA. 122603326@qq.com.|College of Arts and Sciences, Gonzaga University, Spokane, 99258, USA. 122603326@qq.com.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Malisani
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, 200080, China. xixiaowei1966@126.com.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xi
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China. weihan@sjtu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital, Tong Ji University School of Medicine, No.536, Changle Road, Shanghai, 200080, China. wanxp@sjtu.edu.cn.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D001853:Bone Marrow / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D005472:Fluorouracil / Q000633:toxicity; D006410:Hematopoiesis / Q000187:drug effects*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000235:genetics; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D052243:Resistin / Q000235:genetics* / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D052243:Resistin; C499623:Retn protein, mouse; D005472:Fluorouracil