title: Effect of microfabricated microgroove-surface devices on the morphology of mesenchymal stem cells.
abstract: The surface of a material that is in contact with cells is known to affect cell morphology and function. To develop an appropriate surface for tendon engineering, we used zigzag microgroove surfaces, which are similar to the tenocyte microenvironment. The purpose of this study was to investigate the effect of microgroove surfaces with different ridge angles (RAs), ridge lengths (RLs), ridge widths (RWs), and groove widths (GWs) on human bone marrow-derived mesenchymal stem cell (MSC) shape. Dishes with microgroove surfaces were fabricated using cyclic olefin polymer by injection-compression molding. The other parameters were fixed, and effects of different RAs (180 - 30 °), RLs (5 - 500 μm), RWs (5 - 500 μm), and GWs (5 - 500 μm) were examined. Changes in the zigzag shape of the cell due to different RAs, RLs, RWs, and GWs were observed by optical microscopy and scanning electron microscopy. Cytoskeletal changes were investigated using Phalloidin immunofluorescence staining. As observed by optical microscopy, MSCs changed to a zigzag shape in response to microgroove surfaces with different ridge and groove properties. . As observed by scanning electron microscopy, the cell shape changed at turns in the microgroove surface. Phalloidin immunofluorescence staining indicated that F-actin, not only in cell filopodia but also inside the cell body, changed orientation to conform to the microgrooves. In conclusion, the use of zigzag microgroove surfaces microfabricated by injection-compression molding demonstrated the property of MSCs to alter their shapes to fit the surface.
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan. blue@hs.med.kyoto-u.ac.jp.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.affiliation: Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yasuda
authors.affiliation: Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Oike
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Tajino
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Nagai
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Rune
authors.initials: R
authors.lastname: Fujioka
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Iijima
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Shoki
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Precision Machinery Department, SEIKOH GIKEN Co., Ltd., Chiba, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kakinuma
authors.affiliation: Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuroki
mesh_terms: D048430:Cell Shape; D002478:Cells, Cultured; D004867:Equipment Design; D005455:Fluorescent Antibody Technique; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D055616:Microtechnology; D013499:Surface Properties
keywords: Cell shape; Mesenchymal stem cell; Microgroove; Zigzag