title: Derinat Protects Skin against Ultraviolet-B (UVB)-Induced Cellular Damage.
abstract: Ultraviolet-B (UVB) is one of the most cytotoxic and mutagenic stresses that contribute to skin damage and aging through increasing intracellular Ca(2+) and reactive oxygen species (ROS). Derinat (sodium deoxyribonucleate) has been utilized as an immunomodulator for the treatment of ROS-associated diseases in clinics. However, the molecular mechanism by which Derinat protects skin cells from UVB-induced damage is poorly understood. Here, we show that Derinat significantly attenuated UVB-induced intracellular ROS production and decreased DNA damage in primary skin cells. Furthermore, Derinat reduced intracellular ROS, cyclooxygenase-2 (COX-2) expression and DNA damage in the skin of the BALB/c-nu mice exposed to UVB for seven days in vivo. Importantly, Derinat blocked the transient receptor potential canonical (TRPC) channels (TRPCs), as demonstrated by calcium imaging. Together, our results indicate that Derinat acts as a TRPCs blocker to reduce intracellular ROS production and DNA damage upon UVB irradiation. This mechanism provides a potential new application of Derinat for the protection against UVB-induced skin damage and aging.
authors.affiliation: The Institute of Basic Medical Sciences, Medical of College, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan. mhtsai0522@gmail.com.|Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. mhtsai0522@gmail.com.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. toddherpuma@yahoo.com.tw.
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Lu
authors.affiliation: Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8581, Japan. mami@med.kyushu-u.ac.jp.
authors.forename: Mami
authors.initials: M
authors.lastname: Noda
authors.affiliation: Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. yup.kmu@gmail.com.|Department of Dermatology, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. yup.kmu@gmail.com.
authors.forename: Ching-Ying
authors.initials: CY
authors.lastname: Wu
authors.affiliation: Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8581, Japan. liubeibei.35@163.com.
authors.forename: Jia-Dai
authors.initials: JD
authors.lastname: Liu
authors.affiliation: School of High-Technology for Human Welfare, Tokai University, 410-0321 Numazu, Shizuoka, Japan. msakaki@chime.ocn.ne.jp.
authors.forename: Manabu
authors.initials: M
authors.lastname: Sakakibara
authors.affiliation: Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. mhtsai0522@gmail.com.|Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. mhtsai0522@gmail.com.
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Tsai
authors.affiliation: Department of Dermatology, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. yup.kmu@gmail.com.|National Health Research Institutes, Distinguished Investigator, National Environmental Health Research Center, No. 35, Keyan Road, Zhunan Town, Miaoli County 35053, Taiwan. yup.kmu@gmail.com.
authors.forename: Hsin-Su
authors.initials: HS
authors.lastname: Yu
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. tanukikimo@yahoo.com.tw.|Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. tanukikimo@yahoo.com.tw.
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Lin
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. yabihu@kmu.edu.tw.|Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. yabihu@kmu.edu.tw.
authors.forename: Yaw-Bin
authors.initials: YB
authors.lastname: Huang
authors.affiliation: The Institute of Basic Medical Sciences, Medical of College, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan. DROSOUV@gmail.com.|Department of Physiology, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan. DROSOUV@gmail.com.
authors.forename: Shian-Jang
authors.initials: SJ
authors.lastname: Yan
authors.affiliation: Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. yoshitohru@gmail.com.|Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan. yoshitohru@gmail.com.
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: C541364:Derinat; D020011:Protective Agents; D017382:Reactive Oxygen Species; D004247:DNA; D051546:Cyclooxygenase 2; D002118:Calcium
keywords: Derinat; ROS; TRPCs; UVB; calcium