title: Electrochemical acetylcholinesterase biosensor based on ZnO nanocuboids modified platinum electrode for the detection of carbosulfan in rice.
abstract: The consumption of carbosulfan-contaminated rice affects the immune and lymphocyte response, germinal centers in the spleen, plasma cells in popliteal lymphoid nodes, bone marrow cells and granulocyte-macrophage progenitor cells. Towards this, a highly sensitive acetylcholinesterase (AChE) cyclic voltammetric biosensor based on zinc oxide (ZnO) nanocuboids modified platinum (Pt) electrode has been successfully developed. The Pt/ZnO/AChE/Chitosan bio-electrode was employed for the electrochemical detection of carbosulfan in rice sample. Under optimum conditions, the Pt/ZnO/AChE/Chitosan bio-electrode detected carbosulfan ranging from 5 to 30 nM with a detection limit (LOD) of 0.24 nM. The developed Pt/ZnO/AChE/Chitosan bio-electrode showed good recovery (99.06-100.96%), thus providing a promising tool for analysis of carbosulfan in rice sample.
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur 613401, Tamil Nadu, India; Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur 613401, Tamil Nadu, India; School of Electrical & Electronics Engineering, SASTRA University, Thanjavur 613401, Tamil Nadu, India.
authors.forename: Noel
authors.initials: N
authors.lastname: Nesakumar
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur 613401, Tamil Nadu, India; School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur 613401, Tamil Nadu, India; School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamil Nadu, India.
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.affiliation: Nanosensors Lab, SASTRA University, Thanjavur 613401, Tamil Nadu, India; Centre for Nanotechnology & Advanced Biomaterials, SASTRA University, Thanjavur 613401, Tamil Nadu, India; School of Electrical & Electronics Engineering, SASTRA University, Thanjavur 613401, Tamil Nadu, India. Electronic address: rjbosco@ece.sastra.edu.
authors.forename: John Bosco Balaguru
authors.initials: JB
authors.lastname: Rayappan
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D015374:Biosensing Techniques; D002219:Carbamates / Q000032:analysis* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation; D005506:Food Contamination / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012275:Oryza / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D002219:Carbamates; D010984:Platinum; D000110:Acetylcholinesterase; D015034:Zinc Oxide; C035038:carbosulfan
keywords: Acetylcholinesterase; Carbosulfan; Cyclic voltammetry; Rice sample; Zinc oxide