title: Genetic Variation of BCL2 (rs2279115), NEIL2 (rs804270), LTA (rs909253), PSCA (rs2294008) and PLCE1 (rs3765524, rs10509670) Genes and Their Correlation to Gastric Cancer Risk Based on Universal Tagged Arrays and Fe3O4 Magnetic Nanoparticles.
abstract: With the help of Fe3O4 nagnetic nanoparticles as a solid carrier and an excellent tool for separation, six SNP loci (rs2279115 of BCL2 gene, rs804270 of NEIL2 gene, rs909253 of LTA gene, rs2294008 of PSCA gene, rs3765524 and rs10509670 of PLCE1 gene) were selected to evaluate their relation to gastric cancer risk. Using two kinds of functionalized magnetic nanoparticles and universal tagged arrays, the whole operation procedure including genome DNA extraction and SNP genotyping was performed. All genotypes and allele frequencies were calculated in the cases and controls respectively to analyze their association with gastric cancer risk. Totally 200 pathological samples and 134 normal control subjects were collected. The results demonstrated that four SNP loci (rs2279115, rs804270, rs909253 and rs3765524) showed a potential association with gastric cancer risk, and the other two (rs2294008, rs10509670) possessed no difference/association among cases and controls.
authors.forename: Xianbo
authors.initials: X
authors.lastname: Mou
authors.forename: Taotao
authors.initials: T
authors.lastname: Li
authors.forename: Jiuhai
authors.initials: J
authors.lastname: Wang
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Ali
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Enben
authors.initials: E
authors.lastname: Su
authors.forename: Qiongying
authors.initials: Q
authors.lastname: Jia
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Ni
authors.forename: Daxiang
authors.initials: D
authors.lastname: Cui
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D016022:Case-Control Studies; D045647:DNA Glycosylases / Q000235:genetics*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics*; D058851:GPI-Linked Proteins / Q000235:genetics; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D009363:Neoplasm Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D054801:Phosphoinositide Phospholipase C / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics*
chemical_list: D000951:Antigens, Neoplasm; C000595707:BCL2 protein, human; D058851:GPI-Linked Proteins; D008233:Lymphotoxin-alpha; D058185:Magnetite Nanoparticles; D009363:Neoplasm Proteins; C110843:PSCA protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon; D045647:DNA Glycosylases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; C473642:NEIL2 protein, human