title: Centrifugal Microfluidic System for Nucleic Acid Ampliﬁcation and Detection.
abstract: We report here the development of a rapid PCR microﬂuidic system comprising a double-shaft turntable and centrifugal-based disc that rapidly drives the PCR mixture between chambers set at different temperatures, and the bidirectional flow improved the space utilization of the disc. Three heating resistors and thermistors maintained uniform, specific temperatures for the denaturation, annealing, and extension steps of the PCR. Infrared imaging showed that there was little thermal interference between reaction chambers; the system enabled the cycle number and reaction time of each step to be independently adjusted. To validate the function and efficiency of the centrifugal microfluidic system, a 350-base pair target gene from the hepatitis B virus was ampliﬁed and quantitated by fluorescence detection. By optimizing the cycling parameters, the reaction time was reduced to 32 min as compared to 120 min for a commercial PCR machine. DNA samples with concentrations ranging from 10 to 10⁶ copies/mL could be quantitatively analyzed using this system. This centrifugal-based microfluidic platform is a useful system and possesses industrialization potential that can be used for portable diagnostics.
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. miaobaogang@126.com.
authors.forename: Baogang
authors.initials: B
authors.lastname: Miao
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. pnc@mail.xjtu.edu.cn.
authors.forename: Niancai
authors.initials: N
authors.lastname: Peng
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. lileixjtu@stu.xjtu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. horly@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. hufei0701@yeah.net.
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. zhangzengming@stu.xjtu.edu.cn.
authors.forename: Zengming
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, China. chhw@mail.xjtu.edu.cn.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Wang
keywords: Lab-on-a-disc; PCR; centrifugal microﬂuidics; real-time fluorescence detection