title: Study of distinct serum proteomics for the biomarkers discovery in colorectal cancer.
abstract: To utilize surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to identify potential biomarkers for diagnosis, preoperative pathological classification, staging, and postoperative prognosis in patients with colorectal cancer (CRC), a total of 152 samples were analyzed in this study, including 53 untreated CRC, 12 colorectal adenoma, 15 healthy volunteers, 30 post-treatment CRC patients with stable disease, and 42 post-treatment CRC with progressive disease. The samples were all analyzed by SELDI-TOF-MS and CM10 ProteinChip technology. The proteomic profiles were validated using a bioinformatics tool based on support vector machine (SVM) and undecimated discrete wavelet transform (UDWT) methods. Seven protein peaks were selected as potential biomarkers for CRC, with a specificity of 85.19% and a sensitivity of 96.23%. Four protein peaks were selected as potential markers for colorectal mucinous adenocarcinoma and non-mucinous adenocarcinoma, with a specificity of 95.12% and sensitivity of 83.33%. In addition, SELDI-based serum profiling discriminated between patients with locally advanced (stage I-II) and regionally advanced (stage III) CRC, and between patients with locoregional (stage I-III) and systemic (stage IV) CRC, with high specificity and sensitivity. A protein peak at 5909 Da was identified as a potential marker for tumor progression and prognosis in CRC. In conclusion, we have demonstrated that ProteinChip technology with SELDI-TOF-MS could provide a novel, non-invasive tool for diagnosis, potential preoperative biomarkers for pathological classification, staging, and postoperative prognostic markers for patients with CRC.
authors.affiliation: Department of Radiation Oncology, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.|Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Wenhong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Xuexin
authors.initials: X
authors.lastname: He
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Zhixuan
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pan
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Hong
authors.initials: H
authors.lastname: He
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Weiting
authors.initials: W
authors.lastname: Ge
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Jiekai
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Radiation Oncology, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Qichun
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Yiding
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Cancer Institute, Key Laboratory of Cancer Prevention and Intervention, the Second Affiliated Hospital, Zhejiang University, College of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Suzhan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015179:Colorectal Neoplasms / Q000097:blood*; D006801:Humans; D009362:Neoplasm Metastasis; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D014408:Biomarkers, Tumor