title: MMP-9 gene polymorphisms (rs3918242, rs3918254 and rs4810482) and the risk of psoriasis vulgaris: No evidence for associations in a Chinese Han population.
abstract: Several previous studies including one of them co-authored by our group have revealed that serum and psoriatic plaque expression of matrix metalloproteinase-9 (MMP-9) was significantly upregulated in psoriasis. The aim of this study was to investigate the association of three single nucleotide polymorphisms (SNPs) and haplotypes of MMP-9 (rs3918242, rs3918254 and rs4810482) with psoriasis vulgaris in a Chinese Han population. The serum levels of MMP-9 in 245 psoriasis vulgaris cases and 256 healthy controls were assessed using ELSA kits, and the three SNPs were genotyped using polymerase chain reaction-ligation detection reaction (PCR-LDR) method. Four haplotypes based on the three SNPs were also analyzed. Our study showed that the serum MMP-9 levels in patients with psoriasis vulgaris were significantly higher than that in controls (P<0.05). However, the three SNPs were not significantly associated with psoriasis vulgaris susceptibility (all P>0.05). Similar results were found in further subgroup analysis based on gender, age of onset, family history, and serum MMP-9 levels, except that a protective effect of psoriasis vulgaris was detected among female subjects with the CT genotype of rs3918254 (OR=0.47, 95% CI=0.23-0.96, P=0.038), but this association did not survive after Bonferroni correction (P(adj)=0.076). The haplotype analysis also failed to show any association with psoriasis vulgaris. We found no evidence for the association between the MMP-9 polymorphisms and psoriasis vulgaris susceptibility in a Chinese Han population.
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Jingyao
authors.initials: J
authors.lastname: Liang
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Sanquan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Zhenming
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China.
authors.forename: Rihua
authors.initials: R
authors.lastname: Lin
authors.affiliation: Institute of Dermatology, Guangzhou Medical University, Guangzhou 510095, PR China; Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou 510095, PR China. Electronic address: gzpfbfzs@yahoo.com.
authors.forename: Xibao
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D002648:Child; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D012307:Risk Factors; D055815:Young Adult
chemical_list: D020780:Matrix Metalloproteinase 9
keywords: MMP-9; Psoriasis; Risk; Single nucleotide polymorphisms