title: Rusa alfredi papillomavirus 1 - a novel deltapapillomavirus inducing endemic papillomatosis in the endangered Visayan spotted deer.
abstract: We describe a novel papillomavirus - Rusa alfredi papillomavirus 1 (RalPV1) - which causes endemic fibropapillomatosis in the European conservation breeding population of the highly endangered Visayan spotted deer (Rusa alfredi). Degenerated papillomavirus-specific primers were used to amplify and sequence parts of the viral DNA. Subsequently, the complete genomic DNA was cloned and the sequence was determined. The RalPV1 genome has a length of 8029 bp, encodes the early proteins E6, E7, E1, E2 and E5, the two late proteins L1 and L2 and contains an upstream regulatory region. Highest sequence identities were observed with two deltapapillomaviruses, the Capreolus capreolus PV1 and Cervus elaphus PV1. Pairwise comparisons and phylogenetic analysis based on the ORF L1 suggested that RalPV1 is a putative new type of the papillomavirus species Deltapapillomavirus 5.
authors.affiliation: Institute for Infectious Diseases and Zoonoses, LMU Munich, Veterinärstrasse 13, D-80539 Munich, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Fux
authors.affiliation: Institute for Infectious Diseases and Zoonoses, LMU Munich, Veterinärstrasse 13, D-80539 Munich, Germany.|Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, LMU Munich, Veterinärstrasse 13, D-80539 Munich, Germany.
authors.forename: Martin C
authors.initials: MC
authors.lastname: Langenmayer
authors.affiliation: Zoo Landau in der Pfalz, Hindenburgstrasse 12, D-76829 Landau in der Pfalz, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Jörgens
authors.affiliation: Zoo Landau in der Pfalz, Hindenburgstrasse 12, D-76829 Landau in der Pfalz, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Schubert
authors.affiliation: Zoo Landau in der Pfalz, Hindenburgstrasse 12, D-76829 Landau in der Pfalz, Germany.
authors.forename: Jens-Ove
authors.initials: JO
authors.lastname: Heckel
authors.affiliation: Institute for Infectious Diseases and Zoonoses, LMU Munich, Veterinärstrasse 13, D-80539 Munich, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D003670:Deer / Q000821:virology*; D052687:Deltapapillomavirus / Q000145:classification* / Q000302:isolation & purification*; D019353:Endemic Diseases; D005060:Europe / Q000453:epidemiology; D016679:Genome, Viral; D006651:Histocytochemistry; D008853:Microscopy; D008969:Molecular Sequence Data; D010212:Papilloma / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins