title: A sensitive fluorescence method for detection of E. Coli using rhodamine 6G dyeing.
abstract: Negatively charged bacteria combined with positively charged alkaline dye rhodamine 6G (Rh6G) in NaH2 PO4 -Na2 HPO4 buffer solution pH 7.4, by electrostatic interaction. The dyed bacteria exhibited a strong fluorescence peak at 552 nm and fluorescence intensity was directly linear to Escherichia coli (E. coli), Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aureus) concentrations in the range of 7.06 × 10(4) to 3.53 × 10(7) , 4.95 × 10(5) to 2.475 × 10(8) and 32.5 to 16250 colony forming unit/mL (cfu/mL) respectively, with detection limits of 3.2 × 10(4) cfu/mL E. coli, 2.3 × 10(5) cfu/mL B. subtilis and 16 cfu/mL S. aureus, respectively. Samples were cultured for 12 h, after which the linear detection range for E. coli was 2 to 88 cfu/mL. This simple, rapid and sensitive method was used for the analysis of water and drinking samples. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Caina
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Guiqing
authors.initials: G
authors.lastname: Wen
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Yanghe
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Aimiao
authors.initials: A
authors.lastname: Qin
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry of Education; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000302:isolation & purification; D004926:Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013211:Staphylococcus aureus / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G
keywords: E. coli; Rh6G, dying; fluorescence analysis