title: Reprint of "Pharmacological study of the light/dark preference test in zebrafish (Danio rerio): Waterborne administration".
abstract: Anxiety is a complex disorder; thus, its mechanisms remain unclear. Zebrafish (Danio rerio) are a promising pharmacological model for anxiety research. Light/dark preference test is a behaviorally validated measure of anxiety in zebrafish; however, it requires pharmacological validation. We sought to evaluate the sensitivity of the light/dark preference test in adult zebrafish by immersing them in drug solutions containing clonazepam, buspirone, imipramine, fluoxetine, paroxetine, haloperidol, risperidone, propranolol, or ethanol. The time spent in the dark environment, the latency time to first crossing, and the number of midline crossings were analyzed. Intermediate concentrations of clonazepam administered for 600s decreased the time spent in the dark and increased locomotor activity. Buspirone reduced motor activity. Imipramine and fluoxetine increased time spent in the dark and the first latency, and decreased the number of alternations. Paroxetine did not alter the time in the dark; however, it increased the first latency time and decreased locomotor activity. Haloperidol decreased the time spent in the dark at low concentrations. Risperidone and propranolol did not change any parameters. Ethanol reduced the time spent in the dark and increased the number of crossings at intermediate concentrations. These results corroborate the previous work using intraperitoneal drug administration in zebrafish and rodents, suggesting that water drug delivery in zebrafish can effectively be used as an animal anxiety model.
authors.affiliation: Laboratório de Neurociências e Comportamento, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa, 01, Campus básico, Guamá, CEP: 66075-110 Belém, PA, Brazil. Electronic address: lilianmagno16@gmail.com.
authors.forename: Lílian Danielle Paiva
authors.initials: LD
authors.lastname: Magno
authors.affiliation: Programa de Engenharia Biomédica/COPPE, Universidade Federal do Rio de Janeiro, Avenida Horácio Macedo 2030, Bloco H, CEP: 21945-970 Rio de Janeiro, RJ, Brazil. Electronic address: aldo.fontes@gmail.com.
authors.forename: Aldo
authors.initials: A
authors.lastname: Fontes
authors.affiliation: Laboratório de Neurociências e Comportamento, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa, 01, Campus básico, Guamá, CEP: 66075-110 Belém, PA, Brazil. Electronic address: beatriznecy@hotmail.com.
authors.forename: Beatriz Maria Necy
authors.initials: BM
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Neurociências e Comportamento, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Corrêa, 01, Campus básico, Guamá, CEP: 66075-110 Belém, PA, Brazil; Núcleo de Teoria e Pesquisa do Comportamento, Instituto de Psicologia, Universidade Federal do Pará, Belém, PA, Brazil. Electronic address: gouveiajr.a@gmail.com.
authors.forename: Amauri
authors.initials: A
authors.lastname: Gouveia
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage; D001007:Anxiety / Q000188:drug therapy; D001522:Behavior, Animal / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D008027:Light; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D015027:Zebrafish / Q000502:physiology*
chemical_list: D014151:Anti-Anxiety Agents
keywords: Animal model; Anxiety; Behavior; Drugs; Zebrafish