title: Screening of peroxynitrite scavengers in Flos Lonicerae by using two new methods, an HPLC-DAD-CL technique and a peroxynitrite spiking test followed by HPLC-DAD analysis.
abstract: BACKGROUND
  Peroxynitrite is involved in the pathogenesis of a number of significant diseases. Peroxynitrite scavengers thus have potential application in understanding and treating these diseases. It is, therefore, important to establish screening methods able to rapidly identify peroxynitrite scavengers from herbal plants.
  
  
  OBJECTIVE
  To develop effective and easily operable screening methods for identifying peroxynitrite scavengers in complex matrices, including Chinese herbal medicines.
  
  
  METHODS
  Two simple and efficient screening methods have been developed for the identification of natural peroxynitrite scavengers in Flos Lonicerae Japonicae (FLJ). Method I used HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while Method II used pre-column reaction of the sample with peroxynitrite, followed by HPLC separation and Q-TOF MS/MS analysis.
  
  
  RESULTS
  Five peroxynitrite scavengers (neochlorogenic acid, chlorogenic acid, 3,4-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acid and 4,5-O-dicaffeoyl quinic acid) were identified in FLJ using Method I. Besides the compounds identified using Method I, three additional peroxynitrite scavengers (rutin, isoquercitrin and luteoloside) were identified using Method II.
  
  
  CONCLUSIONS
  The two new methods proved to be complementary and the use of these methods should allow rapid detection of peroxynitrite-scavenging natural products from FLJ and other complex matrices.
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: You-Hua
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, P. R. China.
authors.forename: Yuan-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing, 211198, P. R. China.
authors.forename: Bo-Yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D005960:Glucosides / Q000032:analysis / Q000494:pharmacology; D031239:Lonicera / Q000737:chemistry; D008165:Luminol / Q000737:chemistry; D047311:Luteolin / Q000032:analysis / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000378:metabolism*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D012431:Rutin / Q000032:analysis / Q000494:pharmacology
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; C016527:isoquercitrin; D030421:Peroxynitrous Acid; D002726:Chlorogenic Acid; D008165:Luminol; D012431:Rutin; C066408:luteolin-7-glucoside; D011794:Quercetin; D047311:Luteolin; C100434:3,5-dicaffeoylquinic acid
keywords: Flos Lonicerae Japonicae; HPLC-DAD-CL; HPLC-ESI-Q-TOF MS/MS; peroxynitrite spiking test; peroxynitrite-scavenging activity