title: Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis.
abstract: We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.
authors.affiliation: Department of Chemistry, Center for Molecular Innovation and Drug Discovery, Chemistry of Life Processes Institute, Northwestern University, Silverman Hall, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
authors.forename: Heeseon
authors.initials: H
authors.lastname: An
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Statsyuk
mesh_terms: D002384:Catalysis; D004798:Enzymes / Q000737:chemistry*; D015335:Molecular Probes / Q000138:chemical synthesis*
chemical_list: D004798:Enzymes; D015335:Molecular Probes