title: The Effect of Acute and Chronic Social Stress on the Hippocampal Transcriptome in Mice.
abstract: Psychogenic stress contributes to the formation of brain pathology. Using gene expression microarrays, we analyzed the hippocampal transcriptome of mice subjected to acute and chronic social stress of different duration. The longest period of social stress altered the expression of the highest number of genes and most of the stress-induced changes in transcription were reversible after 5 days of rest. Chronic stress affected genes involved in the functioning of the vascular system (Alas2, Hbb-b1, Hba-a2, Hba-a1), injury response (Vwf, Mgp, Cfh, Fbln5, Col3a1, Ctgf) and inflammation (S100a8, S100a9, Ctla2a, Ctla2b, Lcn2, Lrg1, Rsad2, Isg20). The results suggest that stress may affect brain functions through the stress-induced dysfunction of the vascular system. An important issue raised in our work is also the risk of the contamination of brain tissue samples with choroid plexus. Such contamination would result in a consistent up- or down-regulation of genes, such as Ttr, Igf2, Igfbp2, Prlr, Enpp2, Sostdc1, 1500015O10RIK (Ecrg4), Kl, Clic6, Kcne2, F5, Slc4a5, and Aqp1. Our study suggests that some of the previously reported, supposedly specific changes in hippocampal gene expression, may be a result of the inclusion of choroid plexus in the hippocampal samples.
authors.affiliation: Department of Animal Behaviour, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland.
authors.forename: Adrian M
authors.initials: AM
authors.lastname: Stankiewicz
authors.affiliation: Software Department, Faculty of Computer Science, Bialystok University of Technology, Bialystok, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Goscik
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Majewska
authors.affiliation: Department of Human and Animal Physiology, Institute of Biology, University of Gdansk, Gdansk, Poland.|Department of Pharmacology, Toxicology and Neuroscience, Louisiana State University Health Sciences Center, Shreveport, Louisiana, United States of America.
authors.forename: Artur H
authors.initials: AH
authors.lastname: Swiergiel
authors.affiliation: Department of Animal Behaviour, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, Poland.
authors.forename: Grzegorz R
authors.initials: GR
authors.lastname: Juszczak
mesh_terms: D000208:Acute Disease; D000818:Animals; D002831:Choroid Plexus / Q000378:metabolism; D002908:Chronic Disease; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D005810:Multigene Family; D009929:Organ Size / Q000235:genetics; D012919:Social Behavior; D013154:Spleen / Q000033:anatomy & histology; D013315:Stress, Psychological / Q000235:genetics*; D013950:Thymus Gland / Q000033:anatomy & histology; D013997:Time Factors; D059467:Transcriptome