title: Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.
abstract: Pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem (iPS) cells, are regarded as new sources for cell replacement therapy. These cells can unlimitedly expand under undifferentiated conditions and be differentiated into multiple cell types. Automated culture systems enable the large-scale production of cells. In addition to reducing the time and effort of researchers, an automated culture system improves the reproducibility of cell cultures. In the present study, we newly designed a fully automated cell culture system for human iPS maintenance. Using an automated culture system, hiPS cells maintained their undifferentiated state for 60 days. Automatically prepared hiPS cells had a potency of differentiation into three germ layer cells including dopaminergic neurons and pancreatic cells.
authors.affiliation: Department of Regenerative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Konagaya
authors.affiliation: Global Manufacturing Division, Panasonic Corporation, 2-7 Matsuba-cho, Kadoma City, Osaka 571-8502, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ando
authors.affiliation: Global Manufacturing Division, Panasonic Corporation, 2-7 Matsuba-cho, Kadoma City, Osaka 571-8502, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yamauchi
authors.affiliation: Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Suemori
authors.affiliation: Department of Regenerative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D001331:Automation; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D007621:Karyotyping; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors