title: Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA--a new platform for cirrhosis diagnosis.
abstract: The cirrhotic condition of the liver has long been acknowledged as the preface to liver cancer. The desialylation status of the serum acute phase protein, haptoglobin, has been introduced as a new diagnostic analyte for liver cirrhosis. The reliability of this new diagnostic molecule has been evaluated in 30 liver cirrhosis patients having a history of earlier viral hepatitis C (HCV-LC). A novel enzyme linked lectinosorbent assay has been developed coupled with the plasmon mechanism of gold nanoparticle aggregation as the colorimetric read out which can visually distinguish the cirrhotic liver patients from the normal healthy and hepatitis C controls. The assay can be useful for rapid point-of-care detection, and even an untrained person can execute it without a specialized instrument. This method employs Sambucus nigra agglutinin (SNA) to detect the extent of α-2,6 sialylation of serum haptoglobin, the new diagnostic molecule for liver cirrhosis.
authors.affiliation: Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur 844102, India. get.ppb@gmail.com ppb.mm@jcbose.ac.in.
authors.forename: Partha Pratim
authors.initials: PP
authors.lastname: Bose
authors.forename: Gautam
authors.initials: G
authors.lastname: Mandal
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.forename: Ajay
authors.initials: A
authors.lastname: Duseja
authors.forename: Bishnu Pada
authors.initials: BP
authors.lastname: Chatterjee
mesh_terms: D000328:Adult; D001212:Asialoglycoproteins / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006242:Haptoglobins; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged
chemical_list: D001212:Asialoglycoproteins; D006242:Haptoglobins; C031636:asialohaptoglobin