title: The in vitro antioxidant properties of alcalase hydrolysate prepared from silkie fowl (Gallus gallus) blood protein.
abstract: Two types of proteins including blood plasma protein and blood cell protein were isolated from silkie fowl (Gallus gallus) blood and hydrolyzed using alcalase for 0, 2, 4 and 6 h. The blood plasma protein hydrolysate (BPH) and blood cell protein hydrolysate (BCH) were analyzed for pH value, peptide content and antioxidative properties. The significantly higher peptide contents were observed in BPH than that of BCH, which showed that blood plasma protein was more suitable to hydrolysis by alcalase than blood cell protein. Both BPH and BCH showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and Fe(2+) chelating ability. BPH at 4 h of hydrolysis (BPH4) demonstrated significantly higher antioxidant capacity than those treated by alcalase in most of the assays. The BPH4 was separated using ultra-filtration and assessment of the fractions and indicated that low molecular weight of peptides (< 3 kDa) possessed greater DPPH scavenging activity, Fe(2+) chelating ability and inhibitory activity of lipid peroxidation. These results show that BPH has the potential to be ingredients in the food industry as a replacement of synthetic antioxidants.
authors.affiliation: Department of Nutritional Science, Toko University, Chiayi.
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Cheng
authors.affiliation: Graduate Institute of Animal Science, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: I-Chun
authors.initials: IC
authors.lastname: Lai
authors.affiliation: Graduate Institute of Animal Science, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Liang-Chuan
authors.initials: LC
authors.lastname: Lin
authors.affiliation: School of Veterinary Medicine, Azabu University, Sagamihara, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Sakata
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001713:Biphenyl Compounds; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002614:Chelating Agents; D002645:Chickens / Q000097:blood*; D016166:Free Radical Scavengers; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D010851:Picrates; D013381:Subtilisins / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D001798:Blood Proteins; D002614:Chelating Agents; D016166:Free Radical Scavengers; D010455:Peptides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D013381:Subtilisins
keywords: antioxidant properties; blood protein; hydrolysate; silkie fowl