title: Effect of Dynamic Culture and Periodic Compression on Human Mesenchymal Stem Cell Proliferation and Chondrogenesis.
abstract: We have recently developed a bioreactor that can apply both shear and compressive forces to engineered tissues in dynamic culture. In our system, alginate hydrogel beads with encapsulated human mesenchymal stem cells (hMSCs) were cultured under different dynamic conditions while subjected to periodic, compressive force. A customized pressure sensor was developed to track the pressure fluctuations when shear forces and compressive forces were applied. Compared to static culture, dynamic culture can maintain a higher cell population throughout the study. With the application of only shear stress, qRT-PCR and immunohistochemistry revealed that hMSCs experienced less chondrogenic differentiation than the static group. The second study showed that chondrogenic differentiation was enhanced by additional mechanical compression. After 14 days, alcian blue staining showed more extracellular matrix formed in the compression group. The upregulation of the positive chondrogenic markers such as Sox 9, aggrecan, and type II collagen were demonstrated by qPCR. Our bioreactor provides a novel approach to apply mechanical forces to engineered cartilage. Results suggest that a combination of dynamic culture with proper mechanical stimulation may promote efficient progenitor cell expansion in vitro, thereby allowing the culture of clinically relevant articular chondrocytes for the treatment of articular cartilage defects.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Casey G
authors.initials: CG
authors.lastname: Lim
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Addison S
authors.initials: AS
authors.lastname: Goodley
authors.affiliation: Department of Ophthalmology, Renming Hospital of Wuhan University, Wuhan, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Jesse K
authors.initials: JK
authors.lastname: Placone
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Ferlin
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Bao-Ngoc B
authors.initials: BN
authors.lastname: Nguyen
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA.
authors.forename: Adam H
authors.initials: AH
authors.lastname: Hsieh
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, 3238 Jeong H. Kim Engineering Building, College Park, MD, 20742, USA. jpfisher@umd.edu.
authors.forename: John P
authors.initials: JP
authors.lastname: Fisher
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D020219:Chondrogenesis; D019245:Compressive Strength; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000943:Antigens, Differentiation
keywords: Cartilage; Chondrogenesis; Compression; Differentiation; Dynamic culture; Mesenchymal stem cell