title: Study on 3'-UTR length polymorphism in peripheral blood mononuclear cells of uremia patient.
abstract: OBJECTIVE
  The objective of this study was to measure the 3'-untranslated region (3'-UTR) polymorphism lengths in peripheral blood mononuclear cells (PBMCs) from uremia patients.
  
  
  METHODS
  We sequenced the alternative polyadenylation sites in the 3'-UTR of PBMCs from 10 uremic patients and 10 healthy people to detect different gene expression levels between uremia patients and healthy controls. Quantitative reverse transcription polymerase chain reaction was used as validation.
  
  
  RESULTS
  Compared with the healthy control group, 691 genes in uremic patients had significantly different 3'-UTR lengths. Of these genes, 475 genes showed shortened 3'-UTRs, and the 3'-UTRs of 216 genes were lengthened. The verification results matched the original sequencing results.
  
  
  CONCLUSIONS
  There were significant differences in 3'-UTR lengths between uremic patients and healthy controls, and analysis of the differential genes may contribute to the understanding of uremia pathogenesis.
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Sui
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Can
authors.initials: C
authors.lastname: Zheng
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Minglin
authors.initials: M
authors.lastname: Ou
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Jiejing
authors.initials: J
authors.lastname: Chen
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Li
authors.initials: L
authors.lastname: Dong
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Hou
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Liu
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Wei
authors.affiliation: a Guangxi Key Laboratory of Metabolic Diseases Research, Nephrology Department of Guilin 181st Hospital , Guilin , Guangxi , China and.|b Clinical Medical Research Center, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital , Shenzhen , Guangdong , China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Dai
mesh_terms: D020413:3' Untranslated Regions; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014511:Uremia / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions
keywords: 3′-untranslated region; APA site sequencing; Uremia; length polymorphism