title: Isolation and characterization of a Far-Eastern strain of tick-borne encephalitis virus in China.
abstract: Tick-borne encephalitis virus (TBEV) is a leading cause of human neurological infection in many parts of Europe and Asia. Although several TBEV isolates have been reported, current understanding of the biological characteristics of a Chinese strain is limited. In this study, a Far-Eastern strain of TBEV designated WH2012 was isolated in northern China. Its genome has been sequenced and found to be closely related to other Chinese TBEV isolates. Human cell lines of neural origin exposed to WH2012 showed cytopathic effects and WH2012 replicated most efficiently in human neuroblastoma cells SK-N-SH. In addition, WH2012 possessed a pathogenic potential in the mouse model, characterized by inducing a complete paralysis in the hindlimbs with a fatal outcome. We herein describe the first data regarding biological properties of TBEV from China. This study may help future research on pathogenic mechanisms of the neurological disease induced by TBEV infection in China.
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shu
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Panyong
authors.initials: P
authors.lastname: Mao
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Bingke
authors.initials: B
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Qinxue
authors.initials: Q
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Electronic address: wanghz@wh.iov.cn.
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D002681:China; D016000:Cluster Analysis; D003588:Cytopathogenic Effect, Viral; D004195:Disease Models, Animal; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D051379:Mice; D009474:Neurons / Q000821:virology; D010243:Paralysis; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D016019:Survival Analysis
chemical_list: D012367:RNA, Viral
keywords: China; Encephalitis; Growth curve; Human brain-derived cell lines; Tick-borne encephalitis virus