title: The polymorphism in miR-25 attenuated the oncogenic function in gastric cancer.
abstract: miR-25 was identified as an essential oncogene by promoting the growth and metastasis through TOB1 in gastric cancer (GC). The function of the single nucleotide polymorphism (SNP) located in the mature region of miR-25 (rs41274221) has not been investigated. In this study, we aimed to explore the involvement of rs41274221 in miR-25 in gastric cancer. We found that SNP rs41274221 in miR-25 was participated in the occurrence of GC by acting as a tumor protective factor associating with the tumor growth and metastasis. Besides, further investigation found that upregulation of miR-25 with AA genotype could attenuate the proliferation and invasion of tumor cells caused by wild-type miR-25. The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease.
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of General Surgery, Yixing People's Hospital Affiliated to Jiangsu University, Yixing, 214200, Jiangsu, China. staff030@sina.com.|Yixing People's Hospital (The Affiliated Hospital of Jiangsu University), Yixing, 214200, Jiangsu, China. staff030@sina.com.
authors.forename: Sujun
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000483:Alleles; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D020641:Polymorphism, Single Nucleotide; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; C098669:TOB1 protein, human; D025521:Tumor Suppressor Proteins
keywords: 3′ UTR; Invasion; Proliferation; SNP; miR-25