title: A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells.
abstract: We show that a cage-shaped F-actin network is essential for maintaining a tight spatial organization of Cav1.3 Ca(2+) channels at the synaptic ribbons of auditory inner hair cells. This F-actin network is also found to provide mechanosensitivity to the Cav1.3 channels when varying intracellular hydrostatic pressure. Furthermore, this F-actin mesh network attached to the synaptic ribbons directly influences the efficiency of otoferlin-dependent exocytosis and its sensitivity to intracellular hydrostatic pressure, independently of its action on the Cav1.3 channels. We propose a new mechanistic model for vesicle exocytosis in auditory hair cells where the rate of vesicle recruitment to the ribbons is directly controlled by a synaptic F-actin network and changes in intracellular hydrostatic pressure.
authors.affiliation: Bordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, France.
authors.forename: Philippe Fy
authors.initials: PF
authors.lastname: Vincent
authors.affiliation: Bordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, France.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Bouleau
authors.affiliation: Unité de Génétique et Physiologie de l'Audition, Institut Pasteur, Paris, France.|UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.|Sorbonne Universités, UPMC Université Paris, Paris, France.|Syndrome de Usher et Autres Atteintes Rétino-Cochléaires, Institut de la Vision, Paris, France.|Collège de France, Paris, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Petit
authors.affiliation: Bordeaux Neurocampus, Equipe Neurophysiologie de la Synapse Auditive, Université de Bordeaux, Bordeaux, France.|UMRS 1120, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Dulon
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D005089:Exocytosis; D006199:Hair Cells, Auditory, Inner / Q000502:physiology*; D006874:Hydrostatic Pressure; D008565:Membrane Proteins / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D013569:Synapses / Q000378:metabolism*
chemical_list: D000199:Actins; D008565:Membrane Proteins; C516233:otoferlin protein, mouse
keywords: actin network; cell biology; exocytosis; hair cell; mouse; neuroscience; osmotic pressure