title: Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone.
abstract: A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4'-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N(⁎)), anionic (A(⁎)) and tautomeric (T(⁎)), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A(⁎)/T(⁎)), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A(⁎) band and had almost no effect on the T(⁎) band. As the ratios of the two bands (A(⁎)/T(⁎)) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves.
authors.affiliation: Department of Chemistry, Faculty of Science, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
authors.forename: Asli
authors.initials: A
authors.lastname: Capan
authors.affiliation: Marmara University, Department of Chemical Engineering, 34722 Kadikoy, Istanbul, Turkey.
authors.forename: Muge S
authors.initials: MS
authors.lastname: Bostan
authors.affiliation: TUBITAK UME, Chemistry Group Laboratories, PO Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Mozioglu
authors.affiliation: TUBITAK UME, Chemistry Group Laboratories, PO Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Muslum
authors.initials: M
authors.lastname: Akoz
authors.affiliation: TUBITAK UME, Chemistry Group Laboratories, PO Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Ahmet C
authors.initials: AC
authors.lastname: Goren
authors.affiliation: Marmara University, Department of Chemical Engineering, 34722 Kadikoy, Istanbul, Turkey; TUBITAK UME, Chemistry Group Laboratories, PO Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Mehmet S
authors.initials: MS
authors.lastname: Eroglu
authors.affiliation: Department of Chemistry, Faculty of Science, Istanbul Technical University, Maslak, Istanbul 34469, Turkey; TUBITAK UME, Chemistry Group Laboratories, PO Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Turan
authors.initials: T
authors.lastname: Ozturk
mesh_terms: D001482:Base Composition; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D005419:Flavonoids / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry
chemical_list: D004277:DNA, Single-Stranded; D005419:Flavonoids; D005456:Fluorescent Dyes; D014867:Water; C041477:3-hydroxyflavone
keywords: 3-Hydroxyflavone; DNA; Fluorophore; Specific recognition; Spectroscopic methods