title: Metagenomic analysis of anammox communities in three different microbial aggregates.
abstract: There is great potential to understand the functional diversity of microorganisms that are involved in waste water treatment through metagenomic analyses. This study presents the first metagenomic comparison of taxonomic and functional profiles of the microbial communities occurring in different aggregates from anaerobic ammonium-oxidizing (anammox) bioreactors. The anammox bacterial communities in both biofilm and granule sludge samples showed relatively high abundance and diversity compared with floccular sludge. Four of the five known genera of anammox bacteria were detected in the three cultures except Candidatus Jettenia, which was absent in the granules. Candidatus Kuenenia comprised the major population of anammox bacteria in these three sludges, independent of their growth morphologies. The genome assembled for the Candidatus Kuenenia in the granule was very similar to the published reference genome of Candidatus K. stuttgartiensis. Genes involved in the metabolism of the anammox process were highly detected in the biofilm and granule sludges. In particular, the abundance of hydrazine synthase gene (hzs) in the biofilm was around 486 times more pronounced than that in the granules. The knowledge gained in this study highlights an important role of sludge aggregate in affecting community structure and metabolic potential of anammox systems.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China. gjh@bjut.edu.cn.|Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia. gjh@bjut.edu.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Guo
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Lu
authors.initials: L
authors.lastname: Fan
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Ni
authors.affiliation: Microbiology, IWWR, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.|Department of Biochemistry and Microbiology, Laboratory of Microbiology, Gent University, Gent, 9000, Belgium.
authors.forename: Boran
authors.initials: B
authors.lastname: Kartal
authors.affiliation: Research Department of Microbiology, Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: Microbiology, IWWR, Faculty of Science, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
authors.forename: Mike S M
authors.initials: MS
authors.lastname: Jetten
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China.|Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism*; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D016680:Genome, Bacterial; D056186:Metagenomics; D010084:Oxidation-Reduction; D012722:Sewage / Q000382:microbiology*
chemical_list: D064751:Ammonium Compounds; D012722:Sewage