title: Rough surface Au@Ag core-shell nanoparticles to fabricating high sensitivity SERS immunochromatographic sensors.
abstract: Immunochromatographic sensors (ICSs) are inexpensive, simple, portable, and robust, thus making ICSs commonplace in clinical diagnoses, food testing, and environmental monitoring. However, commonly used gold nanoparticles (AuNPs) ICSs have low sensitivity. Therefore, we developed highly sensitive surface enhanced Raman scattering (SERS) ICSs. To enhance the sensitivity of SERS ICSs, rough surface core-shell Au@Ag nanoparticles (RSAu@AgNPs) were prepared by coating silver on the surface of gold nanoflowers (AuNFs). Then these nanoparticles were used as SERS substrate in the SERS ICSs, after which the SERS ICSs were implemented to detect haemoglobin and heavy metal cadmium ion (Cd(2+)). The limit of detection (LOD) of the SERS ICSs for detecting haemoglobin was 8 ng/mL, and the linear range of the SERS ICSs was from 31.3 to 2000 ng/mL. The LOD of the SERS ICSs for detecting Cd(2+) was 0.05 ng/mL and the linear analysis range was from 0.05 to 25 ng/mL. The cross reactivity of the SERS ICSs was studied and results showed that the SERS ICSs exhibited highly specific for detection of haemoglobin and Cd(2+), respectively. The SERS ICSs were then used to detect haemoglobin (spiked in serum and in stool) and Cd(2+) (spiked in tap water, river water, and soil leaching water), and the results showed high recovery. These characteristics indicated that SERS ICSs were ideal tools for clinical diagnosis and environmental pollution monitoring.
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 982623065@qq.com.
authors.forename: Qiangqiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Integrated Optics and Biophotonics Laboratory, Department of Electronic Engineering, Jinan University, Guangzhou, 510632, People's Republic of China. 422146585@qq.com.
authors.forename: Hongwu Liu
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 525745821@qq.com.
authors.forename: Ze
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Integrated Optics and Biophotonics Laboratory, Department of Electronic Engineering, Jinan University, Guangzhou, 510632, People's Republic of China. 1049511189@qq.com.
authors.forename: An
authors.initials: A
authors.lastname: Liu
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 420652442@qq.com.
authors.forename: Cuize
authors.initials: C
authors.lastname: Yao
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 724220245@qq.com.
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 857367713@qq.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. 308515575@qq.com.
authors.forename: Shiting
authors.initials: S
authors.lastname: Yu
authors.affiliation: Integrated Optics and Biophotonics Laboratory, Department of Electronic Engineering, Jinan University, Guangzhou, 510632, People's Republic of China. zhluocn@gmail.com.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Department of Bioengineering, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com.|Institute of Biotranslational Medicine, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D002104:Cadmium / Q000032:analysis; D002846:Chromatography, Affinity / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013499:Surface Properties
chemical_list: D006454:Hemoglobins; D002104:Cadmium; D012834:Silver; D006046:Gold