title: pCramoll and rCramoll as New Preventive Agents against the Oxidative Dysfunction Induced by Hydrogen Peroxide.
abstract: Oxidative stress plays an important role in the induction of cell death and is associated with various pathologic disorders; therefore, the search for natural products that attenuate the effects produced by oxidant agents is greatly increased. Here, the protective effects of native lectin from Cratylia mollis seeds (pCramoll) and recombinant Cramoll 1 (rCramoll) against H2O2-induced oxidative stress in Vero cells were evaluated. Both lectins significantly attenuated the H2O2-induced cytotoxicity in a concentration-dependent way. The maximum protective effects were 96.85 ± 15.59% (rCramoll) and 59.48 ± 23.44% (pCramoll). The Live/Dead analysis showed a reduction in the percentage of dead cells from 65.04 ± 3.29% (H2O2) to 39.77 ± 2.93% (pCramoll) and 13.90 ± 9.01% (rCramoll). The deleterious effects of H2O2 on cell proliferation were reduced to 10.83% (pCramoll) and 24.17% (rCramoll). Lectins treatment attenuated the excessive superoxide production, the collapse of the mitochondrial membrane potential, and the lysosomal and DNA damage in H2O2-treated cells. In conclusion, our results suggest that pCramoll and rCramoll blocked H2O2-induced cytotoxicity through decreasing reactive oxygen species, restoring the mitochondrial potential, preventing the lysosomal damage and DNA fragmentation, and thus promoting cell survival and proliferation.
authors.affiliation: Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rêgo, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil ; Functional Genomics, Department of Biology, Faculty of Science, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark.
authors.forename: Luís Cláudio Nascimento
authors.initials: LC
authors.lastname: da Silva
authors.affiliation: Laboratório de Biologia Celular, Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Pernambuco, Avenida Professor Moraes Rêgo, s/n, Cidade Universitária, 50670-420 Recife, PE, Brazil.
authors.forename: Neyla Maria Pereira
authors.initials: NM
authors.lastname: Alves
authors.affiliation: Laboratório de Imunogenética, Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Pernambuco, Avenida Professor Moraes Rêgo, s/n, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Laboratório de Parasitologia, Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Rua Alto do Reservatório, s/n, Bela Vista, 55608-680 Vitória de Santo Antão, PE, Brazil.
authors.forename: Maria Carolina Accioly Brelaz
authors.initials: MC
authors.lastname: de Castro
authors.affiliation: Laboratório de Biologia Celular, Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Pernambuco, Avenida Professor Moraes Rêgo, s/n, Cidade Universitária, 50670-420 Recife, PE, Brazil.
authors.forename: Taciana Mirely Maciel
authors.initials: TM
authors.lastname: Higino
authors.affiliation: Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rêgo, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil.
authors.forename: Cássia Regina Albuquerque
authors.initials: CR
authors.lastname: da Cunha
authors.affiliation: Laboratório de Imunogenética, Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Pernambuco, Avenida Professor Moraes Rêgo, s/n, Cidade Universitária, 50670-420 Recife, PE, Brazil.
authors.forename: Valéria Rêgo Alves
authors.initials: VR
authors.lastname: Pereira
authors.affiliation: Laboratório de Agregação de Proteínas e Amiloidoses, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21.941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Nathalia Varejão Nogueira
authors.initials: NV
authors.lastname: da Paz
authors.affiliation: Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rêgo, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil.
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.affiliation: Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rêgo, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil.
authors.forename: Maria Tereza dos Santos
authors.initials: MT
authors.lastname: Correia
authors.affiliation: Laboratório de Biologia Celular, Departamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Pernambuco, Avenida Professor Moraes Rêgo, s/n, Cidade Universitária, 50670-420 Recife, PE, Brazil.
authors.forename: Regina Celia Bressan Queiroz
authors.initials: RC
authors.lastname: de Figueiredo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D004249:DNA Damage / Q000187:drug effects; D007887:Fabaceae / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000633:toxicity*; D037102:Lectins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D012639:Seeds / Q000378:metabolism; D014709:Vero Cells
chemical_list: D037102:Lectins; D020011:Protective Agents; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide