title: Nano-curcumin prepared via supercritical: Improved anti-bacterial, anti-oxidant and anti-cancer efficacy.
abstract: Curcumin (CM) possesses multiple biological activities. However, poor water solubility and low bioavailability limit its application in biomedical fields. CM nanoparticles (NPs) (230-240nm) were prepared by solution-enhanced dispersion via supercritical CO2 (SEDS) (22-22.5MPa pressure, 31-32.5°C temperature) and its biological functions were evaluated in this study. The Minimum inhibitory concentration of CM NPs against S. aureus (∼250μg/mL) was lower than CM-DMSO (∼500μg/mL). Meanwhile, CM NPs showed effective anti-oxidant ability at a concentration raging from 125 to 2000μg/mL. CM NPs showed time-dependent intracellular internalization ability, resulting in an enhanced anti-cancer effect on colorectal cancer cells (HCT116), and the mechanism could be explained by cell cycle arrest in G2/M phase associated with inducing apoptotic cells. Moreover, CM NPs exhibited reduced cytotoxicity on normal cells (NCM460) compared to CM-DMSO and 5-Fu. In conclusion, CM NPs prepared via SEDS showed potentials in biomedical applications.
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK.
authors.forename: Maobin
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Fan
authors.affiliation: State Key Lab of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 43000, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: He
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Aizheng
authors.initials: A
authors.lastname: Chen
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK.
authors.forename: Jiashen
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhi
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK. Electronic address: henry.yili@manchester.ac.uk.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China. Electronic address: lanping@mail.sysu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D000975:Antioxidants / Q000138:chemical synthesis* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002626:Chemistry, Pharmaceutical; D003474:Curcumin / Q000138:chemical synthesis* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D045325:HCT116 Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D003474:Curcumin
keywords: Anti-bacterial; Anti-cancer; Anti-oxidant; Curcumin; Supercritical CO(2)