title: Locus-specific gene repositioning in prostate cancer.
abstract: Genes occupy preferred spatial positions within interphase cell nuclei. However, positioning patterns are not an innate feature of a locus, and genes can alter their localization in response to physiological and pathological changes. Here we screen the radial positioning patterns of 40 genes in normal, hyperplasic, and malignant human prostate tissues. We find that the overall spatial organization of the genome in prostate tissue is largely conserved among individuals. We identify three genes whose nuclear positions are robustly altered in neoplastic prostate tissues. FLI1 and MMP9 position differently in prostate cancer than in normal tissue and prostate hyperplasia, whereas MMP2 is repositioned in both prostate cancer and hyperplasia. Our data point to locus-specific reorganization of the genome during prostate disease.
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Marc
authors.initials: M
authors.lastname: Leshner
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Michelle
authors.initials: M
authors.lastname: Devine
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Roloff
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA 98195.
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: True
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 mistelit@mail.nih.gov meaburnk@mail.nih.gov.
authors.forename: Tom
authors.initials: T
authors.lastname: Misteli
authors.affiliation: National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 mistelit@mail.nih.gov meaburnk@mail.nih.gov.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Meaburn
mesh_terms: D002874:Chromosome Mapping; D022004:Chromosome Structures; D056426:Genetic Loci; D015894:Genome, Human; D006801:Humans; D007399:Interphase / Q000235:genetics; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D051789:Proto-Oncogene Protein c-fli-1 / Q000235:genetics
chemical_list: C078433:FLI1 protein, human; D051789:Proto-Oncogene Protein c-fli-1; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9