title: Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9.
abstract: The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step 'pop in & out' targeting approach facilitates this task.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Robert Rössle Strasse, 13125, Berlin, Germany. ralf.kuehn@mdc-berlin.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Kühn
authors.affiliation: Max Delbrück Center for Molecular Medicine, Robert Rössle Strasse, 13125, Berlin, Germany.
authors.forename: Van Trung
authors.initials: VT
authors.lastname: Chu
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D002460:Cell Line; D018390:Gene Targeting; D016678:Genome / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000235:genetics; D009154:Mutation; D017393:RNA Editing / Q000235:genetics