title: A Functional Single-Nucleotide Polymorphism in Pre-microRNA-196a2 is Associated with Atrial Fibrillation in Han Chinese.
abstract: BACKGROUND
  MicroRNAs are small, single-stranded, non-protein-coding RNAs of about 22 nucleotides. MicroRNA molecules have been identified to play key roles in a broad range of physiologic and pathologic processes. Polymorphisms in the corresponding sequence space are likely to make a significant contribution to phenotypic variation. A T/C genetic variant (rs11614913) in the pre-miR-196a2 sequence could alter mature miR-196a expression and target mRNA binding. The aim of the present study is to evaluate the relationship between this polymorphism and atrial fibrillation (AF).
  
  
  METHODS
  A total of 123 participants were enrolled, 65 AF patients were confirmed with electrocardiogram (ECG) or dynamic electrocardiography, 58 normal individuals were assigned to the control group. Genotypes of the premiR-196a2 were distinguished using the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
  
  
  RESULTS
  The distribution of the pre-miR-196a2 genotypes (TT, TC, and CC) was 15.38%, 46.15%, and 38.46% in the AF group and 39.66%, 46.55%, and 13.79% in the controls, respectively (p = 0.0011). Compared with the TT genotype, the C allele carriers (TC+CC genotypes) had a 3.968-fold increased risk of AF (adjusted OR = 3.968, 95% CI = 1.633 - 9.644, p = 0.002). AF patients with the TC+CC genotype had greater left atrial dimension than did patients with the TT genotype (42.10 ± 8.74 vs. 35.13 ± 8.16, p = 0.0224).
  
  
  CONCLUSIONS
  Our data support that the pre-miR-196a2 polymorphism is associated with AF, and the C allele is a risk factor for AF.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Su
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Guo
authors.forename: Fei
authors.initials: F
authors.lastname: Cai
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Cai
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Pan
authors.forename: Xin-Tao
authors.initials: XT
authors.lastname: Deng
authors.forename: Min
authors.initials: M
authors.lastname: Pan
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001281:Atrial Fibrillation / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003920:Diabetes Mellitus / Q000453:epidemiology; D004562:Electrocardiography; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006325:Heart Atria / Q000473:pathology; D006801:Humans; D006973:Hypertension / Q000453:epidemiology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009929:Organ Size; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs