title: Bacillus ectoiniformanssp. nov., a halotolerant bacterium isolated from deep-sea sediments.
abstract: A halotolerant, Gram-positive bacterium (strain NE-14T), which was isolated from sediment samples of the South China Sea, was subjected to a taxonomic study. Strain NE-14Tgrew well at wide temperature and pH ranges, 10.0-45.0 °C and pH 6-10, with an optimum at 30 °C and pH 8.0, respectively. Growth of strain NE-14Twas observed at total salt concentrations of 0-10 % (w/v) with optimum at 2 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate belongs to the genusBacillus. Strain NE-14Twas related most closely toBacillus shackletoniiLMG 18435T,Bacillus bataviensisLMG 21833T,Bacillus idriensisSMC 4352-2TandBacillus drentensisLMG 21831Twith 16S rRNA gene sequence similarities of 96.2, 95.9, 95.8 and 95.7 %, respectively. DNA-DNA hybridization between strain NE-14TandB. shackletoniiLMG 18435T,B. bataviensisLMG 21833T,B. idriensisSMC 4352-2TandB. drentensisLMG 21831Tgave reassociation values of about 27.4, 22.4, 16.4 and 15.9 %, respectively. The DNA G+C content of strain NE-14Twas 39.2 mol%. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan containedmeso-diaminopimelic acid as the diagnostic diamino acid. The predominant cellular fatty acids of strain NE-14Twere iso-C15 : 0and anteiso-C15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phospholipid. Based on the phenotypic and phylogenetic characteristics, it is proposed that strain NE-14Tbe classified as representing a novel species of the genusBacillus, for which the nameBacillus ectoiniformanssp. nov. is proposed. The type strain is NE-14T( = DSM 28970T= JCM 30397T).
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy & Food Co. Ltd, Shanghai 200436, PR China.|School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Daochen
authors.initials: D
authors.lastname: Zhu
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Changxiao
authors.initials: C
authors.lastname: Xie
authors.affiliation: Institute of Agricultural Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Li
authors.affiliation: School of Environmental Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, PR China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy & Food Co. Ltd, Shanghai 200436, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Hang