title: Construction, Enzyme Response, and Substrate Capacity of a Hyaluronan-Cyclodextrin Supramolecular Assembly.
abstract: A supramolecular assembly was constructed with a cationic cyclodextrin (EICD) and native hyaluronan (HA). The cationic carboxylic ester pendants on HA support hyaluronidase (HAase)-responsive sites and the EICD supports artificial carboxylic esterase responsive sites. Substrate-binding models were investigated by using environment-sensitive fluorescence probes 2-p-toluidino-6-naphthalenesulfoniate sodium (2,6-TNS) and thioflavin T (ThT). On a HA/EICD assembly, EICD was able to bind an anionic substrate and HA and EICD constructed the cationic substrate binding site together. This assembly could be used as a sequential dual-substrate carrier.
authors.affiliation: Department Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.affiliation: Department Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P.R. China.|Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, 300071, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P.R. China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Li
authors.affiliation: Department Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin, 300071, P.R. China. yuliu@nankai.edu.cn.|Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin, 300071, P.R. China. yuliu@nankai.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D043182:Carboxylesterase / Q000378:metabolism*; D002417:Cattle; D003505:Cyclodextrins / Q000737:chemistry / Q000378:metabolism*; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007700:Kinetics
chemical_list: D003505:Cyclodextrins; D003692:Delayed-Action Preparations; D006820:Hyaluronic Acid; D043182:Carboxylesterase; D006821:Hyaluronoglucosaminidase
keywords: cyclodextrins; enzymes; fluorescent probes; substrate binding; supramolecular chemistry