title: Saccharibacillus deserti sp. nov., isolated from desert soil.
abstract: A Gram-stain-positive, facultatively anaerobic bacterial strain, designated WLJ055T, with polar and subpolar flagella was isolated from the top layer of desert soil from Erdos, Inner Mongolia, northern China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain WLJ055T was a member of the genus Saccharibacillus, and shared 97.17-97.24 % 16S rRNA gene sequence similarities with Saccharibacillus sacchari GR21T and Saccharibacillus kuerlensis HR1T. The major polar lipids of strain WLJ055T were diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone, while anteiso-C15 : 0, C16 : 0, iso-C16 : 0, and anteiso-C17 : 0 were the major cellular fatty acids. Its genomic DNA G+C content was 55.5 mol%. DNA-DNA hybridization revealed that strain WLJ055T showed 45 ± 5 % and 40 ± 5 % genomic DNA relatedness with its two closest relatives, S. sacchari GR21T and S. kuerlensis HR1T, respectively. The results of physiological and biochemical tests allowed the discrimination of strain WLJ055T from its phylogenetic relatives. Saccharibacillus deserti sp. nov. is therefore proposed to be a novel species of the genus Saccharibacillus, with strain WLJ055T ( = CGMCC 1.15276T = KCTC 33693T) as the type strain.
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.|College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Ji-Quan
authors.initials: JQ
authors.lastname: Sun
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Wang
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.|Institute of Innovation (Baotou), Peking University, Baotou, Inner Mongolia 014030, PR China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7