title: Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells.
abstract: Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine) for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines [1]. Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]. In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2-10.0 µM long-chain bases and GML were not cytotoxic to DC; 40.0-80.0 µM long-chain bases, but not GML, were cytotoxic for DC; and 80.0 µM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20 mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.
authors.affiliation: Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Mehalick
authors.affiliation: Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Poulsen
authors.affiliation: Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Carol L
authors.initials: CL
authors.lastname: Fischer
authors.affiliation: Department of Oral Pathology, Radiology and Medicine, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Emily A
authors.initials: EA
authors.lastname: Lanzel
authors.affiliation: Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Amber M
authors.initials: AM
authors.lastname: Bates
authors.affiliation: Central Microscopy Research Facility, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Walters
authors.affiliation: Department of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Joseph E
authors.initials: JE
authors.lastname: Cavanaugh
authors.affiliation: College of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Guthmiller
authors.affiliation: Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Georgia K
authors.initials: GK
authors.lastname: Johnson
authors.affiliation: Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA ; Department of Oral Pathology, Radiology and Medicine, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Wertz
authors.affiliation: Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA ; Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
authors.forename: Kim A
authors.initials: KA
authors.lastname: Brogden
keywords: Cytotoxicity; Dendritic cells; Dihydrosphingosine; Glycerol monolaurate; Human oral gingival epithelial keratinocytes; Oral gingival fibroblasts; Oral squamous cell carcinoma cells; Phytosphingosine; Sphingosine