title: Basal Lamina Mimetic Nanofibrous Peptide Networks for Skeletal Myogenesis.
abstract: Extracellular matrix (ECM) is crucial for the coordination and regulation of cell adhesion, recruitment, differentiation and death. Therefore, equilibrium between cell-cell and cell-matrix interactions and matrix-associated signals are important for the normal functioning of cells, as well as for regeneration. In this work, we describe importance of adhesive signals for myoblast cells' growth and differentiation by generating a novel ECM mimetic peptide nanofiber scaffold system. We show that not only structure but also composition of bioactive signals are important for cell adhesion, growth and differentiation by mimicking the compositional and structural properties of native skeletal muscle basal lamina. We conjugated laminin-derived integrin binding peptide sequence, "IKVAV", and fibronectin-derived well known adhesive sequence, "RGD", into peptide nanostructures to provide adhesive and myogenic cues on a nanofibrous morphology. The myogenic and adhesive signals exhibited a synergistic effect on model myoblasts, C2C12 cells. Our results showed that self-assembled peptide nanofibers presenting laminin derived epitopes support adhesion, growth and proliferation of the cells and significantly promote the expression of skeletal muscle-specific marker genes. The functional peptide nanofibers used in this study present a biocompatible and biodegradable microenvironment, which is capable of supporting the growth and differentiation of C2C12 myoblasts into myotubes.
authors.affiliation: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey 06800.
authors.forename: I Ceren
authors.initials: IC
authors.lastname: Yasa
authors.affiliation: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey 06800.
authors.forename: Nuray
authors.initials: N
authors.lastname: Gunduz
authors.affiliation: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey 06800.
authors.forename: Murat
authors.initials: M
authors.lastname: Kilinc
authors.affiliation: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey 06800.
authors.forename: Mustafa O
authors.initials: MO
authors.lastname: Guler
authors.affiliation: Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, Turkey 06800.
authors.forename: Ayse B
authors.initials: AB
authors.lastname: Tekinay
mesh_terms: D000818:Animals; D001485:Basement Membrane; D040761:Biomimetic Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D020869:Gene Expression Profiling; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry; D012038:Regeneration; D013499:Surface Properties; D013501:Surface-Active Agents
chemical_list: D010455:Peptides; D013501:Surface-Active Agents