title: Clinical implications of and lessons learnt from external assessment of Mers-CoV diagnostics.
abstract: With the emergence of new viral infections, it is necessary to set up new target-specific assays, based on existing molecular techniques such as real-time PCR, as quickly as possible. Without these diagnostic tools, the geographical spread of new infections, follow-up of the disease outbreak and analysis of the pathogenesis of the disease are not possible. Therefore, the genomic information of the emerging pathogen, diagnostic protocols and standards allowing quality control need to be available in a few days. This can only be implemented with good quality experienced laboratories having suitable infrastructure to establish in-house assays. Even though these molecular tools are available quickly, challenges still remain with what sample types to select for a proper diagnostic value.
authors.affiliation: a Department of Viroscience , Erasmus MC , Rotterdam , The Netherlands.
authors.forename: Suzan D
authors.initials: SD
authors.lastname: Pas
authors.affiliation: a Department of Viroscience , Erasmus MC , Rotterdam , The Netherlands.
authors.forename: Marion P G
authors.initials: MP
authors.lastname: Koopmans
authors.forename: Matthias
authors.initials: M
authors.lastname: Niedrig
mesh_terms: D018352:Coronavirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D004196:Disease Outbreaks; D006801:Humans; D065207:Middle East Respiratory Syndrome Coronavirus / Q000235:genetics / Q000302:isolation & purification*; D057089:Pathology, Molecular; D014777:Virus Diseases / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology
keywords: Coronavirus; European quality assurance panel; MERS; Middle East respiratory syndrome; SARS; molecular diagnostics; virus