title: A diagnostic tool for malaria based on computer software.
abstract: Nowadays, the gold standard method for malaria diagnosis is a staining of thick and thin blood film examined by expert laboratorists. It requires well-trained laboratorists, which is a time consuming task, and is un-automated protocol. For this study, Maladiag Software was developed to predict malaria infection in suspected malaria patients. The demographic data of patients, examination for malaria parasites, and complete blood count (CBC) profiles were analyzed. Binary logistic regression was used to create the equation for the malaria diagnosis. The diagnostic parameters of the equation were tested on 4,985 samples (703 infected and 4,282 control samples). The equation indicated 81.2% sensitivity and 80.3% specificity for predicting infection of malaria. The positive likelihood and negative likelihood ratio were 4.12 (95% CI = 4.01-4.23) and 0.23 (95% CI = 0.22-0.25), respectively. This parameter also had odds ratios (P value < 0.0001, OR = 17.6, 95% CI = 16.0-19.3). The equation can predict malaria infection after adjust for age, gender, nationality, monocyte (%), platelet count, neutrophil (%), lymphocyte (%), and the RBC count of patients. The diagnostic accuracy was 0.877 (Area under curve, AUC) (95% CI = 0.871-0.883). The system, when used in combination with other clinical and microscopy methods, might improve malaria diagnoses and enhance prompt treatment.
authors.affiliation: Medical Technology Program, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand.
authors.forename: Manas
authors.initials: M
authors.lastname: Kotepui
authors.affiliation: Medical Technology Program, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand.
authors.forename: Kwuntida
authors.initials: K
authors.lastname: Uthaisar
authors.affiliation: Medical Technology Laboratory, Phop Phra Hospital, Phop Phra District, Tak Province, 63160. Thailand.
authors.forename: Bhukdee
authors.initials: B
authors.lastname: Phunphuech
authors.affiliation: Medical Technology Laboratory, Phop Phra Hospital, Phop Phra District, Tak Province, 63160. Thailand.
authors.forename: Nuoil
authors.initials: N
authors.lastname: Phiwklam
mesh_terms: D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D012372:ROC Curve; D012044:Regression Analysis; D015203:Reproducibility of Results; D012984:Software