title: Plant Esterase-Chitosan/Gold Nanoparticles-Graphene Nanosheet Composite-Based Biosensor for the Ultrasensitive Detection of Organophosphate Pesticides.
abstract: As broad-spectrum pesticides, organophosphates (OPs) are widely used in agriculture all over the world. However, due to their neurotoxicity in humans and their increasing occurrence in the environment, there is growing interest in their sensitive and selective detection. This paper reports a new cost-effective plant esterase-chitosan/gold nanoparticles-graphene nanosheet (PLaE-CS/AuNPs-GNs) biosensor for the sensitive detection of methyl parathion and malathion. Highly pure plant esterase is produced from plants at low cost and shares the same inhibition mechanism with OPs as acetylcholinesterase, and then it was used to prepare PLaE-CS/AuNPs-GNs nanocomposites, which were systematically characterized using SEM, TEM, and UV-vis. The PLaE-CS/AuNPs-GNs composite-based biosensor measured as low as 50 ppt (0.19 nM) of methyl parathion and 0.5 ppb (1.51 nM) of malathion (S/N = 3) with a calibration curve up to 200 ppb (760 nM) and 500 ppb (1513.5 nM) for methyl parathion and malathion, respectively. There is also no interference observed from most of common species such as metal ions, inorganic ions, glucose, and citric acid. In addition, its applicability to OPs-contaminated real samples (carrot and apple) was also demonstrated with excellent response recovery. The developed simple, sensitive, and reliable PLaE-CS/AuNPs-GNs composite-based biosensor holds great potential in OPs detection for food and environmental safety.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bao
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Hou
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Danqun
authors.initials: D
authors.lastname: Huo
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University , Chongqing 400030, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Connecticut , 191 Auditorium Road, Unit 3222, Storrs, Connecticut 06269, United States.
authors.forename: Yu
authors.initials: Y
authors.lastname: Lei
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry; D004950:Esterases / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D010940:Plant Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D010940:Plant Proteins; D006046:Gold; D006108:Graphite; D048271:Chitosan; D004950:Esterases
keywords: biosensor; gold nanoparticles; graphene nanosheets; organophosphates; plant esterase