title: Structural measurements and cell line studies of the copper–  PEG–Amikacin complex against Mycobacterium tuberculosis.
abstract: The bacterium responsible for causing tuberculosis is increasing its resistance to antibiotics resulting in new multidrug-resistant Mycobacterium tuberculosis (MDR-TB) and extensively drug-resistant M. tuberculosis (XDR-TB) strains. In this study, several analytical techniques including NMR, FT-ICR, MALDI-MS, and LC–MS are used to study different aspects of the Copper–polyethylene glycol (PEG)–Amikacin complex. The Cu(II) cation and the aggregate formed by PEG serve as a carrier for the antibiotic. Several Cu–PEG–Amikacin complex variations were tested against NIH-NIAID cell lines containing both resistant and nonresistant strains of M. tuberculosis.
authors.affiliation: Chemistry, Valdosta State University, Valdosta, GA 31698, United States
authors.forename: Thomas
authors.initials: T
authors.lastname: Manning
authors.forename: Hatel
authors.initials: H
authors.lastname: Patel
authors.forename: Greg
authors.initials: G
authors.lastname: Wylie
authors.forename: Dennis
authors.initials: D
authors.lastname: Phillips
authors.forename: Jackie
authors.initials: J
authors.lastname: Jarvis
mesh_terms: D000583:Amikacin / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D011092:Polyethylene Glycols; D003300:Copper; D000583:Amikacin