title: Sunflower-type nanogels carrying a quantum dot nanoprobe for both superior gene delivery efficacy and tracing of human mesenchymal stem cells.
abstract: Sunflower-type nanogels carrying the QD 655 nanoprobe can be used for both gene transfection and bioimaging of hMSCs. The entry of sunflower-type nanogels into hMSCs can be possibly controlled by changing the formation of QDs. The physico-chemical properties of sunflower-type nanogels internalized by hMSCs were confirmed by AFM, SEM, TEM, gel retardation, and ζ-potential analyses. The bioimaging capacity was confirmed by confocal laser microscopy, Kodak imaging, and Xenogen imaging. Specifically, we investigated the cytotoxicity of sunflower-type nanogels via SNP analysis. Internalization of sunflower-type nanogels does not cause malfunction of hMSCs.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea.
authors.forename: Se Won
authors.initials: SW
authors.lastname: Yi
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea.
authors.forename: Seong Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea. Electronic address: shshim@cha.ac.kr.
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Shim
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-complex, Sampyeong-Dong, Bundang-gu, Seongam-si, Republic of Korea. Electronic address: pkh0410@cha.ac.kr.
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis / Q000235:genetics; D058948:Cell Tracking / Q000379:methods*; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis / Q000235:genetics; D018076:DNA, Complementary / Q000008:administration & dosage / Q000235:genetics; D004339:Drug Compounding; D011992:Endosomes; D005260:Female; D018628:Gene Dosage; D017930:Genes, Reporter; D006493:Heparin; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008853:Microscopy / Q000379:methods; D000080385:Nanogels; D020442:Poloxamer; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000633:toxicity; D011094:Polyethyleneimine / Q000008:administration & dosage* / Q000633:toxicity; D020641:Polymorphism, Single Nucleotide; D045663:Quantum Dots / Q000032:analysis*; D055758:SOX9 Transcription Factor / Q000032:analysis / Q000235:genetics; D055672:Static Electricity; D014162:Transfection
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D050659:Core Binding Factor Alpha 1 Subunit; D018076:DNA, Complementary; D000080385:Nanogels; C493709:RUNX2 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C413692:polyethylene glycol polyethyleneimine nanogel; D020442:Poloxamer; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D006493:Heparin
keywords: Bioimaging; Gene delivery; Nanogel; Sunflower-type