title: High-affinity interaction between interleukin-11 and S100P protein.
abstract: Interleukin-11 (IL-11) and S100P are oncoproteins co-expressed in numerous cancers, which might favor their interaction during oncogenesis. We have explored the possibility of this interaction by surface plasmon resonance spectroscopy, intrinsic fluorescence, and chemical crosslinking. Recombinant forms of IL-11 and S100P interact with each other under physiological level of calcium ions. IL-11 molecule has at least two S100P-binding sites with dissociation constants of 32 nM and 288 nM, which is 5-13-fold lower than its affinity to extracellular domain of IL-11 receptor subunit α. S100P does not alter IL-11-induced STAT3 activation in HEK293 cells co-expressing IL-11 receptors, but could affect other tumorigenic signaling pathways. The highly specific IL-11 - S100P interaction occurring under physiologically relevant conditions should be taken into consideration upon development of the antineoplastics inhibiting IL-11 signaling.
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Alexei S
authors.initials: AS
authors.lastname: Kazakov
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Andrei S
authors.initials: AS
authors.lastname: Sokolov
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Rastrygina
authors.affiliation: Biocad, Lubuchany, Moscow Region, 142380, Russia.
authors.forename: Valery V
authors.initials: VV
authors.lastname: Solovyev
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Ramis G
authors.initials: RG
authors.lastname: Ismailov
authors.affiliation: Antherix, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Roman V
authors.initials: RV
authors.lastname: Mikhailov
authors.affiliation: Biocad, Lubuchany, Moscow Region, 142380, Russia.
authors.forename: Andrei B
authors.initials: AB
authors.lastname: Ulitin
authors.affiliation: CJSC R-Pharm, Berzarina Str. 19/1, Moscow, 123154, Russia.
authors.forename: Andrey R
authors.initials: AR
authors.lastname: Yakovenko
authors.affiliation: Antherix, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia; Biomirex Inc., 304 Pleasant Str., Watertown, MA, 02472, USA.
authors.forename: Tajib A
authors.initials: TA
authors.lastname: Mirzabekov
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Permyakov
authors.affiliation: Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya Str. 7, Pushchino, Moscow Region, 142290, Russia. Electronic address: permyakov.s@gmail.com.
authors.forename: Sergei E
authors.initials: SE
authors.lastname: Permyakov
mesh_terms: D001665:Binding Sites; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D017370:Interleukin-11 / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; C508611:IL11 protein, human; D017370:Interleukin-11; D009687:Nuclear Proteins; C496889:S100PBP protein, human; D002118:Calcium
keywords: Cancer; Interleukin; Protein–protein interaction; S100 protein; STAT3