title: The association of factor V leiden mutation with recurrent pregnancy loss.
abstract: OBJECTIVE
  To determine the association of factor V Leiden mutation with recurrent pregnancy loss.
  
  
  METHODS
  The case-control study was conducted at the Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan, from January to June 2012, and comprised women of 18 to 45 years of age who had a history of recurrent pregnancy loss, and controls with no history of pregnancy loss. All the subjects belonged to Punjabi ethnic group. Three ml blood was taken from cases and controls and deoxyribonucleic acid was extracted. In order to identify Factor V Leiden mutation, polymerase chain reaction method was utilised combined with the amplification refractory mutation system. Data was analysed using SPSS 17.
  
  
  RESULTS
  Of the 112 subjects, 56(50%) were in each of the two groups. The presence of factor V Leiden mutation among the cases was 3(5.4%) while it was absent among the controls. The mutation was significantly associated with recurrent pregnancy loss (p=0.017).Recurrent pregnancy loss was higher in cases than controls (p=0.001).
  
  
  CONCLUSIONS
  Factor V Leiden mutation, Recurrent pregnancy loss, PCR (Polymerase chain reaction).
authors.affiliation: Department of Pathology, Shaheed Mohtarma Benazir Bhutto Medical College, Lyari, Karachi.
authors.forename: Sumreen
authors.initials: S
authors.lastname: Kashif
authors.affiliation: Combine Military Hospital Cherat, Frontier Medical College, Abbottabad.
authors.forename: Muhammad Ali
authors.initials: MA
authors.lastname: Kashif
authors.affiliation: Department of Pathology, Frontier Medical College, Abbottabad.
authors.forename: Anjum
authors.initials: A
authors.lastname: Saeed
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D009154:Mutation / Q000235:genetics*; D010154:Pakistan; D011247:Pregnancy; D019851:Thrombophilia / Q000150:complications* / Q000235:genetics; D055815:Young Adult
chemical_list: C095381:factor V Leiden; D005165:Factor V
keywords: Factor V Leiden mutation, Recurrent pregnancy loss, PCR (Polymerase chain reaction).