title: Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion.
abstract: The present study investigates the effects of xenotransplantation of Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) in animals after ventral root avulsion. AT-MSC has similar characteristics to bone marrow mesenchymal stem cells (BM-MSCs), such as immunomodulatory properties and expression of neurotrophic factors. In this study, Lewis rats were submitted to surgery for unilateral avulsion of the lumbar ventral roots and received 5 × 10(5) AT-MSCs via the lateral funiculus. Two weeks after cell administration, the animals were sacrificed and the moto neurons, T lymphocytes and cell defense nervous system were analyzed. An increased neuronal survival and partial preservation of synaptophysin-positive nerve terminals, related to GDNF and BDNF expression of AT-MSCs, and reduction of pro-inflammatory reaction were observed. In conclusion, AT-MSCs prevent second phase neuronal injury, since they suppressed lymphocyte, astroglia and microglia effects, which finally contributed to rat motor-neuron survival and synaptic stability of the lesioned motor-neuron. Moreover, the survival of the injected AT- MSCs lasted for at least 14 days. These results indicate that neuronal survival after lesion, followed by mesenchymal stem cell (MSC) administration, might occur through cytokine release and immunomodulation, thus suggesting that AT-MSCs are promising cells for the therapy of neuronal lesions.
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Thiago B
authors.initials: TB
authors.lastname: Ribeiro
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Adriana S S
authors.initials: AS
authors.lastname: Duarte
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.|Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.
authors.forename: Ana Leda F
authors.initials: AL
authors.lastname: Longhini
authors.affiliation: Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Pradella
authors.affiliation: Neuroimmunomodulation Group, Dept. Genetics, Evolution and Bioagents, University of Campinas, Campinas, Brazil.
authors.forename: Alessandro S
authors.initials: AS
authors.lastname: Farias
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Angela C M
authors.initials: AC
authors.lastname: Luzo
authors.affiliation: Dept. of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
authors.forename: Alexandre L R
authors.initials: AL
authors.lastname: Oliveira
authors.affiliation: Hematology and Hemotherapy Center-University of Campinas/Hemocentro-Unicamp, Instituto Nacional de Ciência e Tecnologia do Sangue, Campinas, São Paulo, Brazil.
authors.forename: Sara Teresinha
authors.initials: ST
authors.lastname: Olalla Saad
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation; D000818:Animals; D064593:Heterografts; D006801:Humans; D056747:Immunomodulation; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D009046:Motor Neurons / Q000473:pathology*; D009416:Nerve Regeneration; D000066829:Neuroprotection; D011843:Radiculopathy / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D013116:Spinal Cord / Q000503:physiopathology / Q000637:transplantation*; D013126:Spinal Nerve Roots / Q000503:physiopathology; D013569:Synapses / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016708:Synaptophysin / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D016708:Synaptophysin