title: Classification of reproductive toxicants with diverse mechanisms in the embryonic stem cell test.
abstract: The embryonic stem cell test (EST) is a promising system to detect embryotoxicity in vitro. Recent studies have pointed out some limitations of the EST and suggest that the applicability domain of the EST and its prediction model have to be better defined. Here, eight substances of known reproductive toxicity were tested in the EST under blind conditions. We applied the prediction model to the data of the EST after classifying the substances according to the published criteria. In addition, a simplified classification of the EST results into two classes as an approach to hazard assessment was compared to the European Union Classification, Labelling and Packaging (CLP) Regulation labels of the substances. With one exception, substances that are labeled as reproductive toxicants according to the CLP Regulation were detected as embryotoxic in the EST while substances without label were found to be non-embryotoxic according to the EST.
authors.affiliation: Department of Experimental Toxicology and ZEBET, German Federal Institute for Risk Assessment (BfR), Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Riebeling
authors.forename: Kristin
authors.initials: K
authors.lastname: Fischer
authors.forename: Andreas
authors.initials: A
authors.lastname: Luch
authors.forename: Andrea E M
authors.initials: AE
authors.lastname: Seiler
mesh_terms: D016475:3T3 Cells; D000085:Acetates / Q000633:toxicity; D000090:Acetoacetates / Q000633:toxicity*; D000613:Aminobutyrates / Q000633:toxicity; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D001562:Benzimidazoles / Q000633:toxicity; D019256:Cadmium Chloride / Q000633:toxicity*; D002219:Carbamates / Q000633:toxicity; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000187:drug effects*; D051379:Mice; D010080:Oxazoles / Q000633:toxicity; D010636:Phenols / Q000633:toxicity*; D012965:Sodium Chloride / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*; D000098826:Bisphenol A Compounds
chemical_list: D000085:Acetates; D000090:Acetoacetates; D000613:Aminobutyrates; D001559:Benzhydryl Compounds; D001562:Benzimidazoles; D019256:Cadmium Chloride; D002219:Carbamates; D010080:Oxazoles; D010636:Phenols; D012965:Sodium Chloride; C003121:phosphinothricin; C005217:methyl acetoacetate; C013598:methoxyacetic acid; C006698:carbendazim; C025643:vinclozolin; C006780:bisphenol A; D000098826:Bisphenol A Compounds