title: Carboxymethylation of polysaccharide from Cyclocarya paliurus and their characterization and antioxidant properties evaluation.
abstract: In this study, three chemically carboxymethyl polysaccharides (CM-CPs) were derived from Cyclocarya paliurus polysaccharides. The physicochemical properties and antioxidant activity in vitro of carboxymethyl derivatives were determined. The results of degree of substitution and FT-IR analysis showed the carboxymethylation of polysaccharides were successful. Compared with unmodified polysaccharides, the contents of carbohydrate and protein were decreased while CM-CP3 with highest DS value had more uronic acid. The carboxymethyl derivatives was mainly composed of Ara, Gal, Glc, Man, GalA, with a molecular weight (Mw) of 1.03-1.08 × 10(6)Da. Compared with the native polysaccharide, the CM-CP3 with highest DS and Mw exhibited the highest antioxidant activity in β-carotene-linoleic acid assay. However, the superoxide radical and hydroxyl radical scavenging activity were decreased by CM-CPs. These results demonstrated appropriate carboxymethylation modification could enhance the potential of C. paliurus polysaccharide as oxidation inhibitor.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address: jhxie@ncu.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Ming-Yue
authors.initials: MY
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Life Sciences, Nanchang University, Nanchang 330031, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Nie
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Xie
mesh_terms: D016166:Free Radical Scavengers / Q000737:chemistry*; D019684:Magnoliopsida / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antioxidant activity; Carboxymethylation; Characterization; Cyclocarya paliurus; Polysaccharide