title: Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest.
abstract: BACKGROUND
  An intricate gene regulatory network drives neural crest migration and differentiation. How epigenetic regulators contribute to this process is just starting to be understood.
  
  
  RESULTS
  We found that mutation of med14 or brg1 in zebrafish embryos resulted in a cluster of neural crest cell-related defects. In med14 or brg1 mutants, neural crest cells that form the jaw skeleton were specified normally and migrated to target sites. However, defects in their subsequent terminal differentiation were evident. Transplantation experiments demonstrated that med14 and brg1 are required directly in neural crest cells. Analysis of med14; brg1 double mutant embryos suggested the existence of a strong genetic interaction between members of the Mediator and BAF complexes.
  
  
  CONCLUSIONS
  These results suggest a critical role for Mediator and BAF complex function in neural crest development, and may also clarify the nature of defects in some craniofacial abnormalities.
authors.affiliation: Model Animal Research Center, Nanjing University, 12 Xuefu Road, Nanjing, 210061, Jiangsu, China. xin.lou@nju.edu.cn.|Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada. xin.lou@nju.edu.cn.|Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, M5S 1A8, Ontario, Canada. xin.lou@nju.edu.cn.|Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, 101 College Street, Toronto, M5G 1L7, Ontario, Canada. xin.lou@nju.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Lou
authors.affiliation: Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada. jeff.burrows@utoronto.ca.|Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, M5S 1A8, Ontario, Canada. jeff.burrows@utoronto.ca.
authors.forename: Jeffrey T A
authors.initials: JT
authors.lastname: Burrows
authors.affiliation: Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G 0A4, Canada. ian.scott@sickkids.ca.|Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, M5S 1A8, Ontario, Canada. ian.scott@sickkids.ca.|Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, 101 College Street, Toronto, M5G 1L7, Ontario, Canada. ian.scott@sickkids.ca.
authors.forename: Ian C
authors.initials: IC
authors.lastname: Scott
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks / Q000235:genetics; D007568:Jaw / Q000196:embryology; D007569:Jaw Abnormalities / Q000235:genetics; D008445:Maxillofacial Development / Q000235:genetics* / Q000502:physiology; D056892:Mediator Complex / Q000235:genetics* / Q000378:metabolism; D009432:Neural Crest / Q000166:cytology*; D010012:Osteogenesis / Q000235:genetics* / Q000502:physiology; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C515835:Med14 protein, zebrafish; D056892:Mediator Complex; D029961:Zebrafish Proteins; C516328:smarca4a protein, zebrafish