title: Interaction of Mg with heavy metals (Cu, Cd) in T. aestivum with special reference to oxidative and proline metabolism.
abstract: Little effort has been made to understand the influence of Mg on cellular processes of plant cell during Cu and Cd toxicities. The present work demonstrates the influence of magnesium (Mg) on copper (Cu) and cadmium (Cd) toxicity on Triticum aestivum (Wheat). We measured a range of parameters related to oxidative stress in wheat exposed to Cu or Cd toxicity in media with different concentrations of Mg. Decreasing Mg concentration significantly exacerbated Cu and Cd toxicity and optimum supply of Mg improved the growth and decreased the toxicity-induced oxidative stress (a substantial decline in the amount of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in root and shoot tissues). Activity of antioxidant enzymes-superoxide dismutase (SOD), ascorbae peroxidase (APX), catalase (CAT) was restored upon optimum Mg concentration in the presence of Cu and Cd toxicity. An increase in proline concentration in roots and shoots that was triggered by Cu and Cd exposure was partly reversed. This was due to decline in pyrroline-5-carboxylate synthetase (P5CS) and pyrroline-5-carboxylate reductase (P5CR) activity and enhanced proline dehydrogenase (PDH) activity. In conclusion, decreasing supply of Mg effectively exacerbated the toxicities of Cu and Cd in wheat.
authors.affiliation: Amity Institute of Biotechnology, Amity University, Gautam Budha Nagar, Noida, 201303, India. vijetasingh1@gmail.com.
authors.forename: Vijeta
authors.initials: V
authors.lastname: Singh
authors.affiliation: Department of Botany, Guru Ghasidas University, Bilaspur, 495009, Chhattisgarh, India.
authors.forename: Bhumi Nath
authors.initials: BN
authors.lastname: Tripathi
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India.
authors.forename: Vinay
authors.initials: V
authors.lastname: Sharma
mesh_terms: D060387:Ascorbate Peroxidases / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D006861:Hydrogen Peroxide / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity*; D010084:Oxidation-Reduction / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D011758:Pyrroles / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014908:Triticum / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
chemical_list: D019216:Metals, Heavy; D011758:Pyrroles; D002734:Chlorophyll; C015485:delta-1-pyrroline-5-carboxylate; D002338:Carotenoids; D008315:Malondialdehyde; C037184:chlorophyll b; D011392:Proline; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D008274:Magnesium; D000077194:Chlorophyll A
keywords: Cadmium; Copper; Heavy metals; Oxidative stress; Proline metabolism; Triticum aestivum