title: Extraction, purification and elicitor activities of polysaccharides from Chrysanthemum indicum.
abstract: Polysaccharides isolated from Chrysanthemum indicum were studied for their pathogen-derived resistance against Sclerotium rolfsii sacc in Atractylodis maceocephalae koidz. The total sugar content and monosaccharide analysis were determined by phenol-sulfuric acid method and gas chromatography, and infrared spectroscopy performed for simple structure information. The activities of CAT and POD as protective enzymes in A. maceocephalae leaves were evaluated. The purified polysaccharides exhibited strong CAT and POD activities in inoculated with S. rolfsii in A. macrocephala leaves, attained the maximum value 568.3 Ug(-1)min(-1) and 604.4 Ug(-1)min(-1)respectively. Whereas, when compared with the control plants, 20mg/ml purified polysaccharides exhibited the strongest CAT and POD activities. Notably, the treatments of A. macepcephalae seedlings with C. indicum polysaccharides (CIP) decreased disease index development caused by S. rolfsii. The disease index after 10 days was significantly reduced when the seedlings treated with 20mg/ml CIP, 4.41 compared to the control plants 32.00. Given together, these results indicated that purified polysaccharides derived from C. indicum may be useful as a natural inducer.
authors.affiliation: School of Engineering, Zhejiang A&F University, Hangzhou 311300, PR China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Du
authors.affiliation: Department of Forestry and Biotechnology, State Key Laboratory of Forest Culture Cultivation Base, Natural Medicine Laboratory, Zhejiang A&F University, Hangzhou 311300, PR China. Electronic address: red_flag11@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: Department of Forestry and Biotechnology, State Key Laboratory of Forest Culture Cultivation Base, Natural Medicine Laboratory, Zhejiang A&F University, Hangzhou 311300, PR China.
authors.forename: Dongfang
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Forestry and Biotechnology, State Key Laboratory of Forest Culture Cultivation Base, Natural Medicine Laboratory, Zhejiang A&F University, Hangzhou 311300, PR China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Shool of Agriculture and Food Science, Zhejiang A&F University, Hangzhou 311300, PR China.
authors.forename: Pinyan
authors.initials: P
authors.lastname: Qin
mesh_terms: D002374:Catalase / Q000737:chemistry; D031202:Chrysanthemum / Q000737:chemistry*; D060467:Disease Resistance; D009005:Monosaccharides / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D036226:Seedlings / Q000382:microbiology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D009005:Monosaccharides; D011134:Polysaccharides; D002374:Catalase; D009195:Peroxidase
keywords: Atractylodis maceocephalae koidz; Chrysanthemum indicum polysaccharides; Disease resistance; Protective enzymes; Sclerotium rolfsii sacc