title: Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics.
abstract: The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. Minor quantitative trait loci (QTLs) for whitefly adult survival (AS) and oviposition rate (OR) were identified and some were confirmed in an F2 BC1 population, where they showed increased percentages of explained variance (more than 30%). Bulked segregant analyses on pools of whitefly-resistant and -susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whitefly-resistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.|Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box 16, 6700AA, Wageningen, The Netherlands.|Graduate School Experimental Plant Sciences, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Floor
authors.initials: F
authors.lastname: van den Oever-van den Elsen
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.|Graduate School Experimental Plant Sciences, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Alejandro F
authors.initials: AF
authors.lastname: Lucatti
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.
authors.forename: Sjaak
authors.initials: S
authors.lastname: van Heusden
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.
authors.forename: Colette
authors.initials: C
authors.lastname: Broekgaarden
authors.affiliation: Plant Research International, Business Unit Bioscience, Wageningen University and Research Centre, P.O. Box 16, 6700AA Wageningen, The Netherlands.
authors.forename: Roland
authors.initials: R
authors.lastname: Mumm
authors.affiliation: Laboratory of Entomology, Wageningen University and Research Centre, P.O. Box 16, 6700AA, Wageningen, The Netherlands.
authors.forename: Marcel
authors.initials: M
authors.lastname: Dicke
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700AJ, Wageningen, The Netherlands.
authors.forename: Ben
authors.initials: B
authors.lastname: Vosman
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D016002:Discriminant Analysis; D060467:Disease Resistance / Q000235:genetics*; D008401:Gas Chromatography-Mass Spectrometry; D005838:Genotype; D006430:Hemiptera / Q000502:physiology*; D055442:Metabolome / Q000235:genetics; D055432:Metabolomics; D010058:Oviposition; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000469:parasitology*; D040641:Quantitative Trait Loci / Q000235:genetics; D032322:Solanum / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology*
keywords: Genetic linkage map; life-history; metabolic fingerprinting; parameters; tomato; whitefly