title: GX1-mediated anionic liposomes carrying adenoviral vectors for enhanced inhibition of gastric cancer vascular endothelial cells.
abstract: Gastric cancer is a highly lethal malignancy and its 5-year survival rate remains depressed in spite of multiple treatment options. Targeting drug delivery to tumor vasculature may be a promising strategy for gastric cancer therapy, for it can block the nutrition source of tumor and inhibit the metastasis and invasion in a certain extent. In present study, we have prepared the drug-targeting delivery system of peptide GX1-mediated anionic liposomes carrying adenoviral vectors (GX1-Ad5-AL), in which the tumor suppressor gene of PTEN was integrated into DNA of Ad5 and the GX1 peptide could play targeting role to vascular of gastric cancer. The inhibition ability of GX1-Ad5-AL to human gastric cancer cell lines (SGC-7901) and human umbilical vein endothelial cells (HUVEC) was evaluated by MTT assay. Further, the cell migration assay was carried out in transwell inserts and the cells uptaking of GX1-Ad5-AL was detected by confocal laser scanning microscopy. The experimental results indicated that the average cell proliferation inhibition rates resulted from the drug delivery system of GX1-Ad5-AL in SGC-7901 and HUVEC were 68.36% and 64.13%, respectively which were higher than that resulted from GX1 or Ad5-AL. Meanwhile, results of cell migration experiment demonstrated that GX1-Ad5-AL could significantly suppress the migration of gastric cancer cell of SGC-7901. Moreover, both the imaging from confocal laser scanning microscopy and the quantitative analysis of fluorescence intensity showed that, GX1-Ad5-AL was more easily uptaken by SGC-7901 cells, as compared to Ad5-AL. Therefore, the formulation of GX1-Ad5-AL was effective for enhancing the inhibition effect and suppressing the migration of gastric cancer vascular endothelial cells.
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiong
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Tierong
authors.initials: T
authors.lastname: Bian
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Huang
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Pei
authors.initials: P
authors.lastname: Jing
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Sichuan Medical University, Luzhou, Sichuan 646 000, PR China. Electronic address: zhongzhirong@126.com.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhong
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000838:Anions; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000235:genetics / Q000493:pharmacokinetics; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D010446:Peptide Fragments / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism*
chemical_list: D000838:Anions; D008081:Liposomes; D010446:Peptide Fragments
keywords: Adenoviral vectors; Anionic liposomes; Cell migration; Cell proliferation inhibition; Gastric cancer vascular endothelial cells; Peptide GX1