title: [Homologous Analysis Using Repetitive-sequence-based PCR Typing of Exfoliative Toxin-producing Staphylococcus aureus Isolated from Our Hospital].
abstract: We examined staphylococcal coagulase types and homologous analysis using the DiversiLab repetitive-sequence-based PCR system in exfoliative toxin (ET)-producing Staphylococcus aureus. Twenty-two isolates (17 methicillin-sensitive Staphylococcus aureus (MSSA) and 5 methicillin-resistant Staphylococcus aureus (MRSA) isolates) obtained in our hospital from January 2012 and December 2013 were used. Three groups were classified according to the coagulase types and serotypes of ET. The first group (4 MSSA) showed coagulase type I and ET-A, and the second group (3 MSSA and 2 MRSA) showed coagulase type I and ET-B. The third group (10 MSSA and 3 MRSA) showed coagulase type V and ET-B. An analysis by DiversiLab demonstrated that homology was high in both the first and second groups. The homogenousness was high among the third group isolates except for the ocular isolates. In our hospital, three important groups were present according to a coagulase type and an ET type, and the homology of ocular isolates could be different from other materials isolates.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyamoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Murakami
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishimiya
authors.forename: Koichiro
authors.initials: K
authors.lastname: Suemori
authors.forename: Hisamichi
authors.initials: H
authors.lastname: Tauchi
mesh_terms: D003030:Coagulase / Q000032:analysis; D005083:Exfoliatins / Q000096:biosynthesis*; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003030:Coagulase; D005083:Exfoliatins