title: Novel Vein Patterns in Arabidopsis Induced by Small Molecules.
abstract: The critical role of veins in transporting water, nutrients, and signals suggests that some key regulators of vein formation may be genetically redundant and, thus, undetectable by forward genetic screens. To identify such regulators, we screened more than 5000 structurally diverse small molecules for compounds that alter Arabidopsis (Arabidopsis thaliana) leaf vein patterns. Many compound-induced phenotypes were observed, including vein networks with an open reticulum; decreased or increased vein number and thickness; and misaligned, misshapen, or nonpolar vascular cells. Further characterization of several individual active compounds suggests that their targets include hormone cross talk, hormone-dependent transcription, and PIN-FORMED trafficking.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 (F.C., T.N.);Bloomfield Industries, Inc., Staten Island, New York 10314 (A.D.); andDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, California 92507 (S.C.).
authors.forename: Francine
authors.identifier: http://orcid.org/0000-0003-2769-7058
authors.initials: F
authors.lastname: Carland
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 (F.C., T.N.);Bloomfield Industries, Inc., Staten Island, New York 10314 (A.D.); andDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, California 92507 (S.C.).
authors.forename: Andrew
authors.identifier: http://orcid.org/0000-0002-3188-4529
authors.initials: A
authors.lastname: Defries
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 (F.C., T.N.);Bloomfield Industries, Inc., Staten Island, New York 10314 (A.D.); andDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, California 92507 (S.C.).
authors.forename: Sean
authors.initials: S
authors.lastname: Cutler
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520 (F.C., T.N.);Bloomfield Industries, Inc., Staten Island, New York 10314 (A.D.); andDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, California 92507 (S.C.) timothy.nelson@yale.edu.
authors.forename: Timothy
authors.identifier: http://orcid.org/0000-0001-7600-0275
authors.initials: T
authors.lastname: Nelson
mesh_terms: D000199:Actins / Q000378:metabolism; D017360:Arabidopsis / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D057166:High-Throughput Screening Assays / Q000379:methods; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000187:drug effects; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000199:Actins; D029681:Arabidopsis Proteins; D005030:Ethylenes; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D011494:Protein Kinases; D054852:Small Molecule Libraries; C116363:PIN1 protein, Arabidopsis; C117013:AT5G57090 protein, Arabidopsis; C036216:ethylene; C108270:BRI1 protein, Arabidopsis