title: Enhanced Laser Desorption/Ionization Mass Spectrometric Detection of Gold Nanoparticles in Biological Samples Using the Synergy between Added Matrix and the Gold Core.
abstract: Laser desorption/ionization mass spectrometry (LDI-MS) has been used to detect gold nanoparticles (AuNPs) in biological samples, such as cells and tissues, by ionizing their attached monolayer ligands. Many NP-attached ligands, however, are difficult to ionize by LDI, making it impossible to track these NPs in biological samples. In this work, we demonstrate that concentrations of matrix-assisted LDI (MALDI) matrices an order of magnitude below the values typically used in MALDI can facilitate the selective detection of AuNPs with these ligands, even in samples as complex as cell lysate. This enhanced sensitivity arises from a synergistic relationship between the gold core and the matrix that helps to selectively ionize ligands attached to the AuNPs.
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Alyssa L M
authors.initials: AL
authors.lastname: Marsico
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Gokhan S
authors.initials: GS
authors.lastname: Elci
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Moyano
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Gulen
authors.initials: G
authors.lastname: Yesilbag Tonga
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Bradley
authors.initials: B
authors.lastname: Duncan
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Ryan F
authors.initials: RF
authors.lastname: Landis
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.affiliation: Department of Chemistry, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Richard W
authors.initials: RW
authors.lastname: Vachet
mesh_terms: D002477:Cells / Q000378:metabolism*; D006046:Gold / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000032:analysis*; D015394:Molecular Structure; D050607:Organogold Compounds / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D050607:Organogold Compounds; D006046:Gold