title: Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla).
abstract: Detecting and separating specific effects of contaminants in a multi-stress field context remain a major challenge in ecotoxicology. In this context, the aim of this study was to assess the usefulness of a non-invasive transcriptomic method, by means of a complementary DNA (cDNA) microarray comprising 1000 candidate genes, on caudal fin clips. Fin gene transcription patterns of European eels (Anguilla anguilla) exposed in the laboratory to cadmium (Cd) or a polychloro-biphenyl (PCBs) mixture but also of wild eels from three sampling sites with differing contamination levels were compared to test whether fin clips may be used to detect and discriminate the exposure to these contaminants. Also, transcriptomic profiles from the liver and caudal fin of eels experimentally exposed to Cd were compared to assess the detection sensitivity of the fin transcriptomic response. A similar number of genes were differentially transcribed in the fin and liver in response to Cd exposure, highlighting the detection sensitivity of fin clips. Moreover, distinct fin transcription profiles were observed in response to Cd or PCB exposure. Finally, the transcription profiles of eels from the most contaminated site clustered with those from laboratory-exposed fish. This study thus highlights the applicability and usefulness of performing gene transcription assays on non-invasive tissue sampling in order to detect the in situ exposure to Cd and PCBs in fish.
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France.|CNRS, EPOC, UMR 5805, F-33400, Talence, France.
authors.forename: Lucie
authors.initials: L
authors.lastname: Baillon
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France. f.pierron@epoc.u-bordeaux1.fr.|CNRS, EPOC, UMR 5805, F-33400, Talence, France. f.pierron@epoc.u-bordeaux1.fr.
authors.forename: Fabien
authors.initials: F
authors.lastname: Pierron
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France.|CNRS, EPOC, UMR 5805, F-33400, Talence, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Oses
authors.affiliation: Institut National de la Recherche Scientifique, Centre Eau Terre Environnement, 490 de la Couronne, Québec, QC, G1K 9A9, Canada.
authors.forename: Pauline
authors.initials: P
authors.lastname: Pannetier
authors.affiliation: Département de biologie, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC, G1V 0A6, Canada.
authors.forename: Eric
authors.initials: E
authors.lastname: Normandeau
authors.affiliation: Institut National de la Recherche Scientifique, Centre Eau Terre Environnement, 490 de la Couronne, Québec, QC, G1K 9A9, Canada.
authors.forename: Patrice
authors.initials: P
authors.lastname: Couture
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France.|CNRS, EPOC, UMR 5805, F-33400, Talence, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Labadie
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France.|CNRS, EPOC, UMR 5805, F-33400, Talence, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: Irtsea, UR EABX, 50 avenue de Verdun-Gazinet, 33612, Cestas, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Lambert
authors.affiliation: Département de biologie, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC, G1V 0A6, Canada.
authors.forename: Louis
authors.initials: L
authors.lastname: Bernatchez
authors.affiliation: Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France.|CNRS, EPOC, UMR 5805, F-33400, Talence, France.
authors.forename: Magalie
authors.initials: M
authors.lastname: Baudrimont
mesh_terms: D000811:Anguilla / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000032:analysis* / Q000494:pharmacology; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D014874:Water Pollutants, Chemical; D002104:Cadmium; D011078:Polychlorinated Biphenyls
keywords: European eel; Multi-pollutant; Non-invasive sampling; Transcriptomics; cDNA microarray