title: Molecular Effects of Irradiation (Cobalt-60) on the Control of Panonychus citri (Acari: Tetranychidae).
abstract: The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p < 0.05) effects on the adult survival, fecundity and egg viability of Panonychus citri. The irradiation caused the degradation of the DNA of P. citri adults and damaged the plasma membrane system of the egg, which led to condensed nucleoli and gathered yolk. Additionally, the transcriptomes and gene expression profiles between irradiated and non-irradiated mites were compared, and three digital gene expression libraries were assembled and analyzed. The differentially expressed genes were putatively involved in apoptosis, cell death and the cell cycle. Finally, the expression profiles of some related genes were studied using quantitative real-time PCR. Our study provides valuable information on the changes in the transcriptome of irradiated P. citri, which will facilitate a better understanding of the molecular mechanisms that cause the sterility induced by irradiation.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. wengweng@scau.edu.cn.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. luolingyan0915@gmail.com.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. xtchan@outlook.com.
authors.forename: Xieting
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. humy@scau.edu.cn.
authors.forename: Meiying
authors.initials: M
authors.lastname: Hu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. hqbscau@126.com.
authors.forename: Qiongbo
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China. lianggong@scbg.ac.cn.
authors.forename: Liang
authors.initials: L
authors.lastname: Gong
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of China, South China Agricultural University, Guangzhou 510642, China. huabao@scau.edu.cn.
authors.forename: Qunfang
authors.initials: Q
authors.lastname: Weng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D016923:Cell Death / Q000528:radiation effects; D016000:Cluster Analysis; D003037:Cobalt Radioisotopes / Q000009:adverse effects; D004249:DNA Damage / Q000528:radiation effects; D005720:Gamma Rays; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects; D010063:Ovum / Q000528:radiation effects / Q000648:ultrastructure; D012098:Reproduction / Q000528:radiation effects; D040102:Tetranychidae / Q000235:genetics* / Q000528:radiation effects*; D059467:Transcriptome
chemical_list: D003037:Cobalt Radioisotopes
keywords: Panonychus citri; apoptosis pathway; gene expression profile; radiation-induced; sterility exposure