title: Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma.
abstract: A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.
authors.affiliation: Gifu Pharmaceutical University.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Murakami
authors.forename: Erina
authors.initials: E
authors.lastname: Otani
authors.forename: Tomoko
authors.initials: T
authors.lastname: Iwata
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Esaka
authors.forename: Takuma
authors.initials: T
authors.lastname: Aoyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Toshiki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Shinya
authors.initials: S
authors.lastname: Minatoguchi
authors.forename: Bunji
authors.initials: B
authors.lastname: Uno
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000302:isolation & purification*; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
chemical_list: D000241:Adenosine