title: Ichthyobacterium seriolicida gen. nov., sp. nov., a member of the phylum 'Bacteroidetes', isolated from yellowtail fish (Seriola quinqueradiata) affected by bacterial haemolytic jaundice, and proposal of a new family, Ichthyobacteriaceae fam. nov.
abstract: A novel Gram-stain-negative, rod-shaped (0.3 × 4-6 μm), non-flagellated, aerobic strain with gliding motility, designated JBKA-6T, was isolated in 1991 from a yellowtail fish, Seriola quinqueradiata, showing symptoms of bacterial haemolytic jaundice. 16S rRNA gene sequence analysis showed that strain JBKA-6T was related most closely to members of the family Flavobacteriaceae in the phylum 'Bacteroidetes'. Furthermore, based on gyrB gene sequence analysis, JBKA-6T was classified into a single clade within the order Flavobacteriales, which was distinct from the known clades of the families Flavobacteriaceae, Blattabacteriaceae and Cryomorphaceae. The predominant isoprenoid quinone was identified as MK-6 (97.9 %), and the major cellular fatty acids (>10 %) were C14 : 0 and iso-C15 : 0. The main polar lipids were phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminophospholipids and two unidentified polar lipids. The DNA G+C content of JBKA-6T, as derived from its whole genome, was 33.4 mol%. The distinct phylogenetic position and phenotypic traits of strain JBKA-6T distinguish it from all other described species of the phylum 'Bacteroidetes', and therefore it was concluded that strain JBKA-6T represents a new member of the phylum 'Bacteroidetes', and the name Ichthyobacterium seriolicida gen. nov., sp. nov. is proposed. The type strain of Ichthyobacterium seriolicida is JBKA-6T ( = ATCC BAA-2465T = JCM 18228T). We also propose that Icthyobacterium gen. nov. is the type genus of a novel family, Ichthyobacteriaceae fam. nov.
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Takano
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Matsuyama
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Sakai
authors.affiliation: National Research Institute of Fisheries Science,Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa, 236-8648,Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamaishi
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Chihaya
authors.initials: C
authors.lastname: Nakayasu
authors.affiliation: Laboratory of Genome Science,Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477,Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Laboratory of Genome Science,Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477,Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
authors.affiliation: Fisheries Research Division,Oita Prefectural Agriculture, Forestry and Fisheries Research Center, 194-6 Tsuiura, Kamiura, Saiki, Oita, 879-2602,Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Fukuda
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Sorimachi
authors.affiliation: National Research Institute of Aquaculture,Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-ise, Mie 516-0193,Japan.
authors.forename: Takaji
authors.initials: T
authors.lastname: Iida
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005393:Fish Diseases / Q000382:microbiology; D007565:Jaundice / Q000382:microbiology; D010473:Perciformes / Q000382:microbiology*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6