title: Stochastic model explains formation of cell arrays on H/O-diamond patterns.
abstract: Cell migration plays an important role in many biological systems. A relatively simple stochastic model is developed and used to describe cell behavior on chemically patterned substrates. The model is based on three parameters: the speed of cell movement (own and external), the probability of cell adhesion, and the probability of cell division on the substrate. The model is calibrated and validated by experimental data obtained on hydrogen- and oxygen-terminated patterns on diamond. Thereby, the simulations reveal that: (1) the difference in the cell movement speed on these surfaces (about 1.5×) is the key factor behind the formation of cell arrays on the patterns, (2) this difference is provided by the presence of fetal bovine serum (validated by experiments), and (3) the directional cell flow promotes the array formation. The model also predicts that the array formation requires mean distance of cell travel at least 10% of intended stripe width. The model is generally applicable for biosensors using diverse cells, materials, and structures.
authors.affiliation: Department of Thin Films and Nanostructures, Institute of Physics ASCR, v.v.i., Cukrovarnická 10, 16200 Prague, Czech Republic.
authors.forename: Egor
authors.initials: E
authors.lastname: Ukraintsev
authors.affiliation: Institute of Inherited Metabolic Disorders, Charles University, Ke Karlovu 2, 12853 Prague, Czech Republic.
authors.forename: Antonin
authors.initials: A
authors.lastname: Broz
authors.affiliation: Institute of Inherited Metabolic Disorders, Charles University, Ke Karlovu 2, 12853 Prague, Czech Republic and Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, alej Svobody 76, 32300 Pilsen, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Hubalek Kalbacova
authors.affiliation: Institute of Physics ASCR, v.v.i., Cukrovarnická 10, 16200 Prague, Czech Republic.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kromka
authors.affiliation: Institute of Physics ASCR, v.v.i., Cukrovarnická 10, 16200 Prague, Czech Republic and Faculty of Electrical Engineering, Czech Technical University, Technická 2, 16627 Prague, Czech Republic.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rezek
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018130:Diamond / Q000737:chemistry*; D006801:Humans; D008124:Locomotion; D008954:Models, Biological; D015233:Models, Statistical; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties
chemical_list: D018130:Diamond