title: The use of Resonant X-ray Emission Spectroscopy (RXES) for the electronic analysis of metal complexes and their interactions with biomolecules.
abstract: This review presents a new application of Resonant X-ray Emission Spectroscopy (RXES) to study the mechanism of action of metal containing anticancer derivatives and in particular platinum in situ and in vivo. The technique is an example of a photon-in photon-out X-ray spectroscopic approach, which enables chemical speciation of drugs to be determined and therefore to derive action mechanisms, and to determine drug binding rates under physiological conditions and therapeutic concentrations. This is made feasible due to the atomic specificity and high penetration depth of RXES. The review presents examples of the three main types of information that can be obtained by RXES and establishes an experimental protocol to perfect the measurements within cells.
authors.affiliation: Department of Chemistry, Ångström Laboratory, Uppsala University, Sweden; Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland. Electronic address: jacinto.sa@kemi.uu.se.
authors.forename: Jacinto
authors.initials: J
authors.lastname: Sá
authors.affiliation: Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland; Paul Scherrer Institute (PSI), 5232 Villigen, Switzerland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czapla-Masztafiak
authors.affiliation: Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Lipiec
authors.affiliation: Paul Scherrer Institute (PSI), 5232 Villigen, Switzerland.
authors.forename: Yves
authors.initials: Y
authors.lastname: Kayser
authors.affiliation: Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kwiatek
authors.affiliation: Centre for Biospectroscopy, School of Chemistry, Monash University, 3800 Victoria, Australia.
authors.forename: Bayden
authors.initials: B
authors.lastname: Wood
authors.affiliation: School of Chemistry, Faculty of Science, Monash University, 3800 Victoria, Australia.
authors.forename: Glen B
authors.initials: GB
authors.lastname: Deacon
authors.affiliation: Laboratoire de Chimie Pharmaceutique Organique, Campus Plaine CP205/5, Université Libre de Bruxelles, Bd du Triomphe, B1050 Brussels, Belgium.
authors.forename: Gilles
authors.initials: G
authors.lastname: Berger
authors.affiliation: Laboratoire de Chimie Pharmaceutique Organique, Campus Plaine CP205/5, Université Libre de Bruxelles, Bd du Triomphe, B1050 Brussels, Belgium.
authors.forename: François
authors.initials: F
authors.lastname: Dufrasne
authors.affiliation: Department of Chemistry, Ångström Laboratory, Uppsala University, Sweden.
authors.forename: Daniel L A
authors.initials: DL
authors.lastname: Fernandes
authors.affiliation: Paul Scherrer Institute (PSI), 5232 Villigen, Switzerland; Institute of Physics, Jan Kochanowski University in Kielce, 25-406 Kielce, Poland. Electronic address: jakub.szlachetko@psi.ch.
authors.forename: Jakub
authors.initials: J
authors.lastname: Szlachetko
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D056831:Coordination Complexes / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D009369:Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy / Q000378:metabolism; D011859:Radiography; D013052:Spectrometry, X-Ray Emission / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes