title: The effects of TRIM5α polymorphism on HIV-2ROD and SIVmac239 replication in PBMCs from Chinese rhesus macaques and Vietnamese-origin cynomolgus macaques.
abstract: Because of the difficulty of obtaining Indian-origin rhesus macaques, Chinese-origin rhesus macaques (CR) and Vietnamese-origin cynomolgus macaques (CM) are now used frequently in HIV/AIDS research. Nonetheless, the effects of TRIM5α polymorphism on viral replication in both CR and CM are unclear. To address these questions, we recruited 70 unrelated CR and 40 unrelated CM and studied the effect of TRIM5α polymorphism on HIV-2ROD and SIVmac239 replication in PBMCs. We found that 3 polymorphisms, located in the B30.2 domain of CR TRIM5α formed a haplotype and affected HIV-2ROD replication. In addition, we found that the variant Y178H, located in the Coiled-coil domain of CM TRIM5α, affected TRIM5α-mediated HIV-2ROD restriction. Finally, two polymorphisms, located in the Coiled-coil domain, altered anti-SIVmac239 activity in CR. We concluded that, CM TRIM5α polymorphism could alter HIV-2ROD infection; however, a different domain of CR TRIM5α was responsible for restricting different virus replication.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China.
authors.forename: Feng-Liang
authors.initials: FL
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Ya-Bin
authors.initials: YB
authors.lastname: Jin
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Deng
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Bei-Lei
authors.initials: BL
authors.lastname: Liu
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhuo
authors.affiliation: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China. Electronic address: zhengyt@mail.kiz.ac.cn.
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: fling@scut.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002681:China; D004195:Disease Models, Animal; D005838:Genotype; D015498:HIV-2 / Q000254:growth & development*; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000821:virology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000254:growth & development*; D014744:Vietnam; D014779:Virus Replication; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D002352:Carrier Proteins
keywords: Chinese-origin rhesus macaques; HIV-2ROD; SIVmac239; TRIM5α polymorphism; Vietnamese-origin cynomolgus macaques