title: Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo.
abstract: TRIM27 (tripartite motif-containing 27) was originally identified as a fusion partner with the RET (REarranged during transfection) proto-oncogene and is highly expressed in various tumor cells and tissues. However, the level of expression and function of TRIM27 in ovarian cancer remain unclear. Here we have measured the expression of TRIM27 in normal ovarian and fallopian tube epithelial cells and in ovarian serous carcinoma cells and correlated TRIM27 expression with clinical and pathological parameters. In addition, we detected the effect of TRIM27 knockdown on proliferation of ovarian cancer cells in cell culture and xenografts. The results demonstrated that TRIM27 was highly expressed in ovarian serous carcinoma cells, and TRIM27 expression was significantly correlated with metastasis and FIGO stage in ovarian serous carcinoma patients. Downregulation of TRIM27 expression suppressed the proliferation of ovarian cancer cells in cell culture and inhibited the growth of xenografts in nude mice. TRIM27 knockdown induced cell cycle arrest and apoptosis in ovarian cancer cells by upregulating the expression of p-P38 and downregulating the expression of p-AKT. Thus the present study suggests that TRIM27 could have important roles as an oncogene during the development of ovarian cancer and could serve as a diagnostic and therapeutic target.
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Gynecology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.|Department of Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Zengtao
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Experimental Therapeutics, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Bast
authors.affiliation: Department of Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Zhanying
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Gynecology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.|Department of Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, P. R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Lining
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D000070584:Tripartite Motif Proteins
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; C000715470:MAS1 protein, human; C064419:TRIM27 protein, human; D000070584:Tripartite Motif Proteins