title: Behavioral and metabolic effects of sublethal doses of two insecticides, chlorpyrifos and methomyl, in the Egyptian cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae).
abstract: Insecticides have long been used as the main method in limiting agricultural pests, but their widespread use has resulted in environmental pollution, development of resistances, and biodiversity reduction. The effects of insecticides at low residual doses on both the targeted crop pest species and beneficial insects have become a major concern. In particular, these low doses can induce unexpected positive (hormetic) effects on pest insects, such as surges in population growth exceeding what would have been observed without pesticide application. Methomyl and chlorpyrifos are two insecticides commonly used to control the population levels of the cotton leafworm Spodoptera littoralis, a major pest moth. The aim of the present study was to examine the effects of sublethal doses of these two pesticides, known to present a residual activity and persistence in the environment, on the moth physiology. Using a metabolomic approach, we showed that sublethal doses of methomyl and chlorpyrifos have a systemic effect on the treated insects. We also demonstrated a behavioral disruption of S. littoralis larvae exposed to sublethal doses of methomyl, whereas no effects were observed for the same doses of chlorpyrifos. Interestingly, we highlighted that sublethal doses of both pesticides did not induce a change in acetylcholinesterase activity in head of exposed larvae.
authors.affiliation: Bioassay Research Department, Central Agricultural Pesticides Laboratory (CAPL), Sabahia Research Station, Agricultural Research Center (ARC), Sabahia, Baccous, P.O. Box 21616, Alexandria, Egypt.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Dewer
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France.
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Pottier
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France.
authors.forename: Lisa
authors.initials: L
authors.lastname: Lalouette
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Maria
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Dacher
authors.affiliation: INRA, Laboratoire de Toxicologie Environnementale, UR 406 A&E, 228 Route de l'Aérodrome, CS 40509, 84914, Avignon Cedex 9, France.
authors.forename: Luc P
authors.initials: LP
authors.lastname: Belzunces
authors.affiliation: INRA, Laboratoire de Toxicologie Environnementale, UR 406 A&E, 228 Route de l'Aérodrome, CS 40509, 84914, Avignon Cedex 9, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Kairo
authors.affiliation: Université de Rennes 1, UMR CNRS 6553 Ecobio 263 Avenue du Gal Leclerc, CS 74205, 35042, Rennes, France.
authors.forename: David
authors.initials: D
authors.lastname: Renault
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Maibeche
authors.affiliation: Institute of Ecology and Environmental Sciences of Paris (iEES Paris) - Sensory Ecology Department - UMR UPMC 113, CNRS, IRD, INRA, PARIS 7, Sorbonne Universités, UPMC Univ Paris 06, UPEC - 7 Quai Saint Bernard, F-75005, Paris, France. david.siaussat@upmc.fr.
authors.forename: David
authors.initials: D
authors.lastname: Siaussat
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D004390:Chlorpyrifos / Q000494:pharmacology*; D004534:Egypt; D004785:Environmental Pollutants / Q000494:pharmacology*; D006458:Hemolymph / Q000378:metabolism; D059165:Hormesis; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D007928:Lethal Dose 50; D008724:Methomyl / Q000494:pharmacology*; D018411:Spodoptera / Q000187:drug effects / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D008724:Methomyl; D000110:Acetylcholinesterase; D004390:Chlorpyrifos
keywords: Chlorpyrifos; Crop pest; Insect behavior; Insecticide; Methomyl; Olfaction; Spodoptera littoralis; Sublethal doses