title: Polydopamine-Gelatin as Universal Cell-Interactive Coating for Methacrylate-Based Medical Device Packaging Materials: When Surface Chemistry Overrules Substrate Bulk Properties.
abstract: Despite its widespread application in the fields of ophthalmology, orthopedics, and dentistry and the stringent need for polymer packagings that induce in vivo tissue integration, the full potential of poly(methyl methacrylate) (PMMA) and its derivatives as medical device packaging material has not been explored yet. We therefore elaborated on the development of a universal coating for methacrylate-based materials that ideally should reveal cell-interactivity irrespective of the polymer substrate bulk properties. Within this perspective, the present work reports on the UV-induced synthesis of PMMA and its more flexible poly(ethylene glycol) (PEG)-based derivative (PMMAPEG) and its subsequent surface decoration using polydopamine (PDA) as well as PDA combined with gelatin B (Gel B). Successful application of both layers was confirmed by multiple surface characterization techniques. The cell interactivity of the materials was studied by performing live-dead assays and immunostainings of the cytoskeletal components of fibroblasts. It can be concluded that only the combination of PDA and Gel B yields materials possessing similar cell interactivities, irrespective of the physicochemical properties of the underlying substrate. The proposed coating outperforms both the PDA functionalized and the pristine polymer surfaces. A universal cell-interactive coating for methacrylate-based medical device packaging materials has thus been realized.
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University , Krijgslaan 281 S4Bis, Ghent B-9000, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Van De Walle
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University , Krijgslaan 281 S4Bis, Ghent B-9000, Belgium.
authors.forename: Ine
authors.initials: I
authors.lastname: Van Nieuwenhove
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University , Krijgslaan 281 S4Bis, Ghent B-9000, Belgium.
authors.forename: Els
authors.initials: E
authors.lastname: Vanderleyden
authors.affiliation: Tissue Engineering Group, Department of Basic Medical Sciences, Ghent University , De Pintelaan 185 6B3, Ghent B-9000, Belgium.
authors.forename: Heidi
authors.initials: H
authors.lastname: Declercq
authors.affiliation: Tissue Engineering Group, Department of Basic Medical Sciences, Ghent University , De Pintelaan 185 6B3, Ghent B-9000, Belgium.
authors.forename: Karolien
authors.initials: K
authors.lastname: Gellynck
authors.affiliation: Center for Microsystems Technology (CMST), Imec and Ghent University , Technologiepark 914A, B-9052 Ghent, Belgium.
authors.forename: David
authors.initials: D
authors.lastname: Schaubroeck
authors.affiliation: B-PHOT Brussels Photonics Team, Department of Applied Physics and Photonics, Vrije Universiteit Brussels , Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Heidi
authors.initials: H
authors.lastname: Ottevaere
authors.affiliation: B-PHOT Brussels Photonics Team, Department of Applied Physics and Photonics, Vrije Universiteit Brussels , Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Hugo
authors.initials: H
authors.lastname: Thienpont
authors.affiliation: Department of Molecular Biotechnology, Ghent University , Coupure links 653, 9000 Ghent, Belgium.|Department of Veterinary Sciences, Antwerp University , Groenenborgerlaan 171, 2020 Antwerp, Belgium.
authors.forename: Winnok H
authors.initials: WH
authors.lastname: De Vos
authors.affiliation: Tissue Engineering Group, Department of Basic Medical Sciences, Ghent University , De Pintelaan 185 6B3, Ghent B-9000, Belgium.
authors.forename: Maria
authors.initials: M
authors.lastname: Cornelissen
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University , Krijgslaan 281 S4Bis, Ghent B-9000, Belgium.|B-PHOT Brussels Photonics Team, Department of Applied Physics and Photonics, Vrije Universiteit Brussels , Pleinlaan 2, 1050 Brussels, Belgium.|Department of Chemistry, University of Antwerp , Universiteitsplein 1, BE-2610 Wilrijk-Antwerp, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Van Vlierberghe
authors.affiliation: Polymer Chemistry & Biomaterials Research Group, Department of Organic and Macromolecular Chemistry, Ghent University , Krijgslaan 281 S4Bis, Ghent B-9000, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Dubruel
mesh_terms: D004864:Equipment and Supplies; D005780:Gelatin / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D019064:Product Packaging / Q000379:methods; D013499:Surface Properties
chemical_list: D007211:Indoles; D008689:Methacrylates; D011108:Polymers; C568283:polydopamine; D011092:Polyethylene Glycols; D005780:Gelatin; D019904:Polymethyl Methacrylate