title: New reassortant H5N8 highly pathogenic avian influenza virus from waterfowl in Southern China.
abstract: New reassortant H5N8 highly pathogenic avian influenza viruses were isolated from waterfowl in Southern China. Blast analysis demonstrated that the PB2 gene in these viruses were most closely related to A/wild duck/Shangdong/628/2011 (H5N1), while their NP genes were both more closely related to A/wild duck/Shandong/1/2011 (H5N1) and A/duck/Jiangsu/k1203/2010 (H5N8). However, the HA, NA, PB1, PA, M, and NS genes had the highest identity with A/duck/Jiangsu/k1203/2010 (H5N8). Phylogenetic analysis revealed that their HA genes belonged to the same GsGd H5 clade 2.3.4.4 detected in China in 2010. Therefore, we supposed that these H5N8 viruses might be novel reassortant viruses that have a H5N8 backbone while acquiring PB2 and NP genes from H5N1 viruses. This study is useful for better understanding the genetic and antigenic evolution of H5 avian influenza viruses in Southern China.
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Yafen
authors.initials: Y
authors.lastname: Song
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Ye
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; College of Life Science, South China Agricultural University Guangzhou, China.
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Siyu
authors.initials: S
authors.lastname: Wu
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Xu
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Peirong
authors.initials: P
authors.lastname: Jiao
authors.affiliation: National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou, China ; Key Laboratory of Animal Vaccine Development, Ministry of Agriculture Guangzhou, China ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Guangzhou, China ; College of Veterinary Medicine, South China Agricultural University Guangzhou, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
keywords: H5N8; Southern China; highly pathogenic avian influenza virus; reassortant; waterfowl