title: Does residual H2O2result in inhibitory effect on enhanced anaerobic digestion of sludge pretreated by microwave-H2O2pretreatment process?
abstract: This study investigated the effects of residual H2O2on hydrolysis-acidification and methanogenesis stages of anaerobic digestion after microwave-H2O2(MW-H2O2) pretreatment of waste activated sludge (WAS). Results showed that high sludge solubilization at 35-45 % was achieved after pretreatment, while large amounts of residual H2O2remained and refractory compounds were thus generated with high dosage of H2O2(0.6 g H2O2/g total solids (TS), 1.0 g H2O2/g TS) pretreatment. The residual H2O2not only inhibited hydrolysis-acidification stage mildly, such as hydrolase activity, but also had acute toxic effect on methanogens, resulting in long lag phase, low methane yield rate, and no increase of cumulative methane production during the 30-day BMP tests. When the low dosage of H2O2at 0.2 g H2O2/g TS was used in MW-H2O2pretreatment, sludge anaerobic digestion was significantly enhanced. The cumulative methane production increased by 29.02 %, but still with a lag phase of 1.0 day. With removing the residual H2O2by catalase, the initial lag phase of hydrolysis-acidification stage decreased from 1.0 to 0.5 day.
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Jibao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Ruilai
authors.initials: R
authors.lastname: Jia
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. yswei@rcees.ac.cn.
authors.forename: Yuansong
authors.initials: Y
authors.lastname: Wei
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Junya
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Shenyang Academy of Environmental Science, Shenyang, 110016, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Cai
mesh_terms: D000693:Anaerobiosis; D006861:Hydrogen Peroxide; D008697:Methane; D008872:Microwaves; D012722:Sewage; D014865:Waste Disposal, Fluid
chemical_list: D012722:Sewage; D006861:Hydrogen Peroxide; D008697:Methane
keywords: Anaerobic digestion; Enzyme activity; Hydrogen peroxide; Inhibition; Microwave; Sludge