title: Excitation energy transfer and charge separation are affected in Arabidopsis thaliana mutants lacking light-harvesting chlorophyll a/b binding protein Lhcb3.
abstract: The composition of LHCII trimers as well as excitation energy transfer and charge separation in grana cores of Arabidopsis thaliana mutant lacking chlorophyll a/b binding protein Lhcb3 have been investigated and compared to those in wild-type plants. In grana cores of lhcb3 plants we observed increased amounts of Lhcb1 and Lhcb2 apoproteins per PSII core. The additional copies of Lhcb1 and Lhcb2 are expected to substitute for Lhcb3 in LHCII trimers M as well as in the LHCII "extra" pool, which was found to be modestly enlarged as a result of the absence of Lhcb3. Time-resolved fluorescence measurements reveal a deceleration of the fast phase of excitation dynamics in grana cores of the mutant by ~15 ps, whereas the average fluorescence lifetime is not significantly altered. Monte Carlo modeling predicts a slowing down of the mean hopping time and an increased stabilization of the primary charge separation in the mutant. Thus our data imply that absence of apoprotein Lhcb3 results in detectable differences in excitation energy transfer and charge separation.
authors.affiliation: Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Adamiec
authors.affiliation: Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gibasiewicz
authors.affiliation: Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614 Poznań, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Luciński
authors.affiliation: Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Giera
authors.affiliation: Department of Solid State Theory, Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Chełminiak
authors.affiliation: Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Szewczyk
authors.affiliation: Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, ul. Umultowska 85, 61-614 Poznań, Poland.
authors.forename: Weronika
authors.initials: W
authors.lastname: Sipińska
authors.affiliation: Department of Physics and Astronomy, VU University, De Boelelaan 1081,1081 HV Amsterdam, The Netherlands.
authors.forename: Rienk
authors.initials: R
authors.lastname: van Grondelle
authors.affiliation: Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614 Poznań, Poland. Electronic address: grzesiek@amu.edu.pl.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jackowski
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004735:Energy Transfer; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes / Q000235:genetics / Q000378:metabolism*; D009010:Monte Carlo Method; D009154:Mutation; D010788:Photosynthesis; D055503:Protein Multimerization; D013050:Spectrometry, Fluorescence; D020524:Thylakoids / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes
keywords: Charge separation; Excitation energy transfer; Light Harvesting Complex II trimers; Monte Carlo simulation; Photosystem II