title: Serotonin Promotes Development and Regeneration of Spinal Motor Neurons in Zebrafish.
abstract: In contrast to mammals, zebrafish regenerate spinal motor neurons. During regeneration, developmental signals are re-deployed. Here, we show that, during development, diffuse serotonin promotes spinal motor neuron generation from pMN progenitor cells, leaving interneuron numbers unchanged. Pharmacological manipulations and receptor knockdown indicate that serotonin acts at least in part via 5-HT1A receptors. In adults, serotonin is supplied to the spinal cord mainly (90%) by descending axons from the brain. After a spinal lesion, serotonergic axons degenerate caudal to the lesion but sprout rostral to it. Toxin-mediated ablation of serotonergic axons also rostral to the lesion impaired regeneration of motor neurons only there. Conversely, intraperitoneal serotonin injections doubled numbers of new motor neurons and proliferating pMN-like progenitors caudal to the lesion. Regeneration of spinal-intrinsic serotonergic interneurons was unaltered by these manipulations. Hence, serotonin selectively promotes the development and adult regeneration of motor neurons in zebrafish.
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK.
authors.forename: Antón
authors.initials: A
authors.lastname: Barreiro-Iglesias
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK.
authors.forename: Karolina S
authors.initials: KS
authors.lastname: Mysiak
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK.
authors.forename: Angela L
authors.initials: AL
authors.lastname: Scott
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK; Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence at the TU Dresden, Fetscherstraße 105, 01307 Dresden, Germany.
authors.forename: Michell M
authors.initials: MM
authors.lastname: Reimer
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK; Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh EH16 4SB, UK. Electronic address: catherina.becker@ed.ac.uk.
authors.forename: Catherina G
authors.initials: CG
authors.lastname: Becker
authors.affiliation: Centre for Neuroregeneration, Edinburgh Medical School, Biomedical Sciences, The Chancellor's Building, University of Edinburgh, Edinburgh EH16 4SB, UK; Euan MacDonald Centre for MND Research, University of Edinburgh, Edinburgh EH16 4SB, UK. Electronic address: thomas.becker@ed.ac.uk.
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
mesh_terms: D000818:Animals; D007395:Interneurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009416:Nerve Regeneration; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D013116:Spinal Cord / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D015027:Zebrafish
chemical_list: D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin