title: High-resolution complementary chemical imaging of bio-elements in Caenorhabditis elegans.
abstract: Here, we present a sub-μm multimodal approach to image essential elements in Caenorhabditis elegans. A combination of chemical imaging technologies reveals total metal concentration, chemical state and the protein to which an element is associated. This application of distinct yet complementary chemical imaging techniques provided unique insight into essential and trace elements at the subcellular level.
authors.affiliation: Elemental Bio-imaging Facility, University of Technology Sydney, Broadway, New South Wales 2007, Australia and The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia. gmccoll@florey.edu.au.
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Hare
authors.affiliation: Australian Synchrotron, Clayton, Victoria 3168, Australia and ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, 3086, Australia.
authors.forename: Michael W M
authors.initials: MW
authors.lastname: Jones
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia. gmccoll@florey.edu.au.
authors.forename: Verena C
authors.initials: VC
authors.lastname: Wimmer
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia. gmccoll@florey.edu.au.
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Jenkins
authors.affiliation: Australian Synchrotron, Clayton, Victoria 3168, Australia.
authors.forename: Martin D
authors.initials: MD
authors.lastname: de Jonge
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia. gmccoll@florey.edu.au.
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
authors.affiliation: The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Victoria 3010, Australia. gmccoll@florey.edu.au.
authors.forename: Gawain
authors.initials: G
authors.lastname: McColl
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008670:Metals / Q000737:chemistry* / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*
chemical_list: D029742:Caenorhabditis elegans Proteins; D008670:Metals