title: TANGO1 recruits ERGIC membranes to the endoplasmic reticulum for procollagen export.
abstract: Previously we showed that membrane fusion is required for TANGO1-dependent export of procollagen VII from the endoplasmic reticulum (ER) (Nogueira, et al., 2014). Along with the t-SNARE Syntaxin 18, we now reveal the complete complement of SNAREs required in this process, t-SNAREs BNIP1 and USE1, and v-SNARE YKT6. TANGO1 recruits YKT6-containing ER Golgi Intermediate Compartment (ERGIC) membranes to procollagen VII-enriched patches on the ER. Moreover residues 1214-1396, that include the first coiled coil of TANGO1, specifically recruit ERGIC membranes even when targeted to mitochondria. TANGO1 is thus pivotal in concentrating procollagen VII in the lumen and recruiting ERGIC membranes on the cytoplasmic surface of the ER. Our data reveal that growth of a mega transport carrier for collagen export from the ER is not by acquisition of a larger patch of ER membrane, but instead by addition of ERGIC membranes to procollagen-enriched domains of the ER by a TANGO1-mediated process.
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: António J M
authors.initials: AJ
authors.lastname: Santos
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Ishier
authors.initials: I
authors.lastname: Raote
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Margherita
authors.initials: M
authors.lastname: Scarpa
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Brouwers
authors.affiliation: Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain.|Universitat Pompeu Fabra, Barcelona, Spain.|Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain.
authors.forename: Vivek
authors.initials: V
authors.lastname: Malhotra
mesh_terms: D051784:Aryl Hydrocarbon Receptor Nuclear Translocator / Q000378:metabolism*; D002460:Cell Line; D024143:Collagen Type VII / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D021381:Protein Transport
chemical_list: C497396:ARNT protein, human; D024143:Collagen Type VII; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator
keywords: cell biology; collagen secretion; human; membrane transport; vesicle biogenesis