title: Carriage of Enterobacteria Producing Extended-Spectrum β-Lactamases and Composition of the Gut Microbiota in an Amerindian Community.
abstract: Epidemiological and individual risk factors for colonization by enterobacteria producing extended-spectrum beta-lactamases (E-ESBL) have been studied extensively, but whether such colonization is associated with significant changes in the composition of the rest of the microbiota is still unknown. To address this issue, we assessed in an isolated Amerindian Guianese community whether intestinal carriage of E-ESBL was associated with specificities in gut microbiota using metagenomic and metatranscriptomic approaches. While the richness of taxa of the active microbiota of carriers was similar to that of noncarriers, the taxa were less homogeneous. In addition, species of four genera, Desulfovibrio, Oscillospira, Parabacteroides, and Coprococcus, were significantly more abundant in the active microbiota of noncarriers than in the active microbiota of carriers, whereas such was the case only for species of Desulfovibrio and Oscillospira in the total microbiota. Differential genera in noncarrier microbiota could either be associated with resistance to colonization or be the consequence of the colonization by E-ESBL.
authors.affiliation: Unidad Mixta de Investigación en Genómica y Salud de la Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública) y el Institut Cavanilles de Biodiversitat y Biología Evolutiva (Universitat de València), Valencia, Spain CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gosalbes
authors.affiliation: Unidad Mixta de Investigación en Genómica y Salud de la Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública) y el Institut Cavanilles de Biodiversitat y Biología Evolutiva (Universitat de València), Valencia, Spain CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Vázquez-Castellanos
authors.affiliation: EA 3964 Bacterial resistance in vivo, University Paris-Diderot Medical School and APHP, Paris, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Angebault
authors.affiliation: EA 3964 Bacterial resistance in vivo, University Paris-Diderot Medical School and APHP, Paris, France.
authors.forename: Paul-Louis
authors.initials: PL
authors.lastname: Woerther
authors.affiliation: EA 3964 Bacterial resistance in vivo, University Paris-Diderot Medical School and APHP, Paris, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Ruppé
authors.affiliation: Unidad Mixta de Investigación en Genómica y Salud de la Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública) y el Institut Cavanilles de Biodiversitat y Biología Evolutiva (Universitat de València), Valencia, Spain.
authors.forename: María Loreto
authors.initials: ML
authors.lastname: Ferrús
authors.affiliation: Unidad Mixta de Investigación en Genómica y Salud de la Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública) y el Institut Cavanilles de Biodiversitat y Biología Evolutiva (Universitat de València), Valencia, Spain CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.
authors.forename: Amparo
authors.initials: A
authors.lastname: Latorre
authors.affiliation: EA 3964 Bacterial resistance in vivo, University Paris-Diderot Medical School and APHP, Paris, France andres.moya@uv.es antoine.andremont@bch.aphp.fr.
authors.forename: Antoine
authors.initials: A
authors.lastname: Andremont
authors.affiliation: Unidad Mixta de Investigación en Genómica y Salud de la Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública) y el Institut Cavanilles de Biodiversitat y Biología Evolutiva (Universitat de València), Valencia, Spain CIBER en Epidemiología y Salud Pública (CIBERESP), Madrid, Spain andres.moya@uv.es antoine.andremont@bch.aphp.fr.
authors.forename: Andrés
authors.initials: A
authors.lastname: Moya
mesh_terms: D000328:Adult; D000368:Aged; D002353:Carrier State; D003901:Desulfovibrio / Q000235:genetics / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005616:French Guiana / Q000453:epidemiology; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007198:Indians, North American; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D059467:Transcriptome; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D012336:RNA, Ribosomal, 16S; D001618:beta-Lactamases