title: EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE，VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES.
abstract: BACKGROUND
  Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource.
  
  
  OBJECTIVE
  To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.
  
  
  METHODS
  Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated.
  
  
  RESULTS
  The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05).
  
  
  CONCLUSIONS
  Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China. mahong_ok@163.com.
authors.forename: H
authors.initials: H
authors.lastname: Ma
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China. m18104626703_1@163.com.
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: X
authors.initials: X
authors.lastname: He
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*; D014199:Trehalose / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D014810:Vitamin E; D014199:Trehalose; D001205:Ascorbic Acid