title: Using Ancient Samples in Projection Analysis.
abstract: Projection analysis is a tool that extracts information from the joint allele frequency spectrum to better understand the relationship between two populations. In projection analysis, a test genome is compared to a set of genomes from a reference population. The projection's shape depends on the historical relationship of the test genome's population to the reference population. Here, we explore in greater depth the effects on the projection when ancient samples are included in the analysis. First, we conduct a series of simulations in which the ancient sample is directly ancestral to a present-day population (one-population model), or the ancient sample is ancestral to a sister population that diverged before the time of sampling (two-population model). We find that there are characteristic differences between the projections for the one-population and two-population models, which indicate that the projection can be used to determine whether a test genome is directly ancestral to a present-day population or not. Second, we compute projections for several published ancient genomes. We compare two Neanderthals and three ancient human genomes to European, Han Chinese and Yoruba reference panels. We use a previously constructed demographic model and insert these five ancient genomes to assess how well the observed projections are recovered.
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, Calfornia 94720-3140 mel.yang@berkeley.edu.
authors.forename: Melinda A
authors.identifier: http://orcid.org/0000-0001-9004-7563
authors.initials: MA
authors.lastname: Yang
authors.affiliation: Department of Integrative Biology, University of California, Berkeley, Calfornia 94720-3140.
authors.forename: Montgomery
authors.initials: M
authors.lastname: Slatkin
mesh_terms: D003198:Computer Simulation; D066264:Datasets as Topic; D003710:Demography; D005828:Genetics, Population; D015894:Genome, Human; D023281:Genomics; D006801:Humans; D008957:Models, Genetic
keywords: ancient genomes; human demography; human population genetics; projection