title: Simple and Rapid Immobilization of Coating Polymers on Poly(dimethyl siloxane)-glass Hybrid Microchips by a Vacuum-drying Method.
abstract: A simple and rapid vacuum-drying modification method was applied to several neutral and charged polymers to obtain coating layers for controlling electroosmotic flow (EOF) and suppressing sample adsorption on poly(dimethyl siloxane) (PDMS)-glass hybrid microchips. In the vacuum-dried poly(vinylpyrrolidone) coating, the electroosmotic mobility (μeo) was suppressed from +2.1 to +0.88 × 10(-4) cm(2)/V·s, and the relative standard deviation (RSD) of μeo was improved from 10.2 to 2.5% relative to the bare microchannel. Among several neutral polymers, poly(vinylalcohol) (PVA) and poly(dimethylacrylamide) coatings gave more suppressed and repeatable EOF with RSDs of less than 2.3%. The vacuum-drying method was also applicable to polyanions and polycations to provide accelerated and inversed EOF, respectively, with acceptable RSDs of less than 4.9%. In the microchip electrophoresis (MCE) analysis of bovine serum albumin (BSA) in the vacuum-dried and thermally-treated PVA coating channel, an almost symmetric peak of BSA was obtained, while in the native microchannel a significantly skewed peak was observed. The results demonstrated that the vacuum-dried polymer coatings were effective to control the EOF, and reduced the surface adsorption of proteins in MCE.
authors.affiliation: Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kitagawa
authors.forename: Syo
authors.initials: S
authors.lastname: Nakagawara
authors.forename: Isoshi
authors.initials: I
authors.lastname: Nukatsuka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Hori
authors.forename: Kenji
authors.initials: K
authors.lastname: Sueyoshi
authors.forename: Koji
authors.initials: K
authors.lastname: Otsuka