title: Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS.
abstract: In this study, a method of metabolite profiling based on UPLC-QTOF/MS was developed to analyze Platycodon grandiflorum. In the optimal UPLC, various metabolites, including major platycosides, were separated well in 15 min. The metabolite extraction protocols were also optimized by selecting a solvent for use in the study, the ratio of solvent to sample and sonication time. This method was used to profile two different parts of P. grandiflorum, i.e., the roots of P. grandiflorum (PR) and the stems and leaves of P. grandiflorum (PS), in the positive and negative ion modes. As a result, PR and PS showed qualitatively and quantitatively different metabolite profiles. Furthermore, their metabolite compositions differed according to individual plant samples. These results indicate that the UPLC-QTOF/MS-based profiling method is a good tool to analyze various metabolites in P. grandiflorum. This metabolomics approach can also be applied to evaluate the overall quality of P. grandiflorum, as well as to discriminate the cultivars for the medicinal plant industry.
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. jaewon3@gmail.com.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. jiddung205@chungbuk.ac.kr.
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Ji
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. kimgs0725@korea.kr.
authors.forename: Geum-Soog
authors.initials: GS
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea. kssong@knu.ac.kr.
authors.forename: Kyung-Sik
authors.initials: KS
authors.lastname: Song
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. urspower@korea.kr.
authors.forename: Yurry
authors.initials: Y
authors.lastname: Um
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. kimot@korea.kr.
authors.forename: Ok Tae
authors.initials: OT
authors.lastname: Kim
authors.affiliation: Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 361-763, Korea. leeyi22@cbnu.ac.kr.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Theragen Etex, Suwon 443-270, Korea. changpyo.hong@therabio.kr.
authors.forename: Chang Pyo
authors.initials: CP
authors.lastname: Hong
authors.affiliation: Genomics Division, National Academy of Agricultural Science (NAAS), Jeonju 560-500, Korea. dhshin013@korea.kr.
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Genomics Division, National Academy of Agricultural Science (NAAS), Jeonju 560-500, Korea. chang@korea.kr.
authors.forename: Chang-Kug
authors.initials: CK
authors.lastname: Kim
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. herbin3@korea.kr.
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Lee
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. ay21cay@korea.kr.
authors.forename: Young-Sup
authors.initials: YS
authors.lastname: Ahn
authors.affiliation: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea. dylee0809@korea.kr.
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D055442:Metabolome; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism; D031238:Platycodon / Q000737:chemistry* / Q000378:metabolism; D012503:Saponins / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification*
chemical_list: D012503:Saponins; D012997:Solvents; D014315:Triterpenes; D009828:Oleanolic Acid
keywords: Platycodon grandiflorum; UPLC-QTOF/MS; metabolomics