title: miR-23a binds to p53 and enhances its association with miR-128 promoter.
abstract: Apoptosis plays an important role in cardiac pathology, but the molecular mechanism by which apoptosis regulated remains largely elusive. Here, we report that miR-23a promotes the apoptotic effect of p53 in cardiomyocytes. Our results showed that miR-23a promotes apoptosis induced by oxidative stress. In exploring the molecular mechanism by which miR-23a promotes apoptosis, we found that it sensitized the effect of p53 on miR-128 regulation. It promoted the association of p53 to the promoter region of miR-128, and enhanced the transcriptional activation of p53 on miR-128 expression. miR-128 can downregulate prohibitin expression, and subsequently promote apoptosis. Our data provides novel evidence revealing that miR-23a can stimulate transcriptional activity of p53.
authors.affiliation: Department of Physiology, Shantou University School of Medicine, Shantou 515031, China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Lynn Htet Htet
authors.initials: LH
authors.lastname: Aung
authors.affiliation: Central Research Laboratory, Peking Union Medical College Hospital, Peking Union Medical College &Chinese Academy of Medical Sciences, Beijing, 100730, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Long
authors.affiliation: Department of Physiology, Shantou University School of Medicine, Shantou 515031, China.
authors.forename: Danian
authors.initials: D
authors.lastname: Qin
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Shejuan
authors.initials: S
authors.lastname: An
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Peifeng
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000091402:Prohibitins; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; C557010:MIRN128 microRNA, rat; C000590974:MIRN23a microRNA, rat