title: Insulin induces the expression of FGF2 but does not synergize with it during angiogenesis.
abstract: Cardiovascular and ischemic diseases are often associated with diabetes mellitus and develop due to occlusion of blood vessels leading to the blockage and insufficient blood supply to the target organs. Current therapeutic strategies for treating these pathologies include growth factor-, gene- and stem cell-based therapies. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) have been used in clinical trials to induce blood vessels. On the other hand, increased levels of both these growth factors have been observed with intense insulin therapy in diabetes mellitus patients further leading to increased risk of retinopathy. This suggests the presence of a possible crosstalk between insulin, FGF and VEGF pathways during angiogenesis. In the present work, we report the likely absence of synergistic effect between insulin and FGF-2. This was initially observed at morphological and histological levels using chick embryonic chorioallantoic membrane (CAM) assay and confirmed by analyzing the expression of angiogenesis regulatory genes by semi-quantitative reverse transcriptase PCR (RT-PCR). Absence of combinatorial effect between insulin, FGF-2 and VEGF during angiogenesis was also demonstrated using CAM assay.
authors.affiliation: Developmental Biology Group, MACS-Agharkar Research Institute, Pune 411 004, India. Electronic address: skrishnapatimsc@gmail.com.
authors.forename: Lakshmi Surekha
authors.initials: LS
authors.lastname: Krishnapati
authors.affiliation: Developmental Biology Group, MACS-Agharkar Research Institute, Pune 411 004, India. Electronic address: smghaskadbi@aripune.org.
authors.forename: Surendra
authors.initials: S
authors.lastname: Ghaskadbi
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects* / Q000378:metabolism; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply*; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013997:Time Factors; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
chemical_list: D043925:Angiogenesis Inducing Agents; D007328:Insulin; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2
keywords: Angiogenesis; FGF-2; Insulin; VEGF