title: Application of Aptamers in Histopathology.
abstract: Aptamers are proving to be exceedingly effective in a number of applications. Given the disadvantages of antibodies, such as batch-to-batch variation and cross-reactivity, aptamers have the potential to revolutionize the field of histopathology due to their high specificity and the ease of their synthesis and modification. Here, we describe a chromogenic staining method for paraffin-embedded tissue sections with FITC-labeled aptamers.
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, 3216, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Shigdar
authors.affiliation: Department of Pathology, Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Pathology, Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, China. lifen_w@163.com.
authors.forename: Lifen
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Medicine, Deakin University, Waurn Ponds, VIC, 3216, Australia. wduan@deakin.edu.au.
authors.forename: Wei
authors.initials: W
authors.lastname: Duan
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000941:Antigens / Q000276:immunology / Q000378:metabolism; D052157:Aptamers, Nucleotide; D007150:Immunohistochemistry / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D052157:Aptamers, Nucleotide
keywords: Aptamers; Chemical antibodies; Chromogenic staining; Diagnostics; Histology; Immunohistochemistry; Paraffin-embedded tissues; Pathology