title: Altered Gut Microbiota Composition in Rag1-deficient Mice Contributes to Modulating Homeostasis of Hematopoietic Stem and Progenitor Cells.
abstract: Hematopoietic stem and progenitor cells (HSPCs) can produce all kind of blood lineage cells, and gut microbiota that consists of various species of microbe affects development and maturation of the host immune system including gut lymphoid cells and tissues. However, the effect of altered gut microbiota composition on homeostasis of HSPCs remains unclear. Here we show that compositional change of gut microbiota affects homeostasis of HSPCs using Rag1 (-/-) mice which represent lymphopenic condition. The number and proportions of HSPCs in Rag1 (-/-) mice are lower compared to those of wild types. However, the number and proportions of HSPCs in Rag1 (-/-) mice are restored as the level of wild types through alteration of gut microbiota diversity via transferring feces from wild types. Gut microbiota composition of Rag1 (-/-) mice treated with feces from wild types shows larger proportions of family Prevotellaceae and Helicobacterceae whereas lower proportions of family Lachnospiraceae compared to unmanipulated Rag1 (-/-) mice. In conclusion, gut microbiota composition of lymphopenic Rag1 (-/-) mice is different to that of wild type, which may lead to altered homeostasis of HSPCs.
authors.affiliation: Integrative Biosciences & Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
authors.forename: Ohseop
authors.initials: O
authors.lastname: Kwon
authors.affiliation: Integrative Biosciences & Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
authors.forename: Seungwon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
authors.forename: Ji-Hae
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Integrative Biosciences & Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
authors.forename: Hyekang
authors.initials: H
authors.lastname: Kim
authors.affiliation: Integrative Biosciences & Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea. ; Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea.
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Lee
keywords: Hematopoietic stem and progenitor cells; gut microbiota; lymphopenic state