title: Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins.
abstract: Vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves and other plant materials.
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of life sciences, Yantai University, Yantai 264005, PR China.
authors.forename: Haizhu
authors.initials: H
authors.lastname: Jin
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Sujing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Fang
authors.affiliation: School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China. Electronic address: chunhuawang508@126.com.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address: chxia@yic.ac.cn.
authors.forename: Chuanhai
authors.initials: C
authors.lastname: Xia
mesh_terms: D000327:Adsorption; D047310:Apigenin / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007529:Isoflavones / Q000302:isolation & purification*; D007700:Kinetics; D018515:Plant Leaves / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D005960:Glucosides; D007529:Isoflavones; C477953:isovitexin-4'-O-glucoside; C021329:vitexin rhamnoside; D047310:Apigenin
keywords: Hawthorn leaves; Macroporous resins; Purification; Vitexin-2″-O-rhamnoside; Vitexin-4″-O-glucoside