title: The influence of ALA-mediated photodynamic therapy on secretion of selected growth factors by colon cancer cells in hypoxia-like environment in vitro.
abstract: BACKGROUND
  Photodynamic therapy (PDT) affects the immune system and tumor cells' secretory activity. Hypoxia may limit PDT effects. The aim of this study was to determine the influence of PDT with aminolevulinic acid (ALA-PDT) in a hypoxic-like microenvironment on the secretion of growth factors: GM-CSF (granulocyte and macrophage colony stimulating factor), G-CSF (granulocyte colony-stimulating factor) and FGF (fibroblast growth factor) by experimental models of colon cancer cells in vitro.
  
  
  METHODS
  Sublethal doses of ALA-PDT (ALA of 1000 μM, light fluence 10J/cm(2), power density rate of 1.5mW/cm(2), wavelength 600-720nm) were administered to two colorectal cancer cell lines varying in malignancy potential: SW480 (local malice) and SW620 (high metastatic activity) under normoxia and hypoxia-like environment. Hypoxia-mimic conditions were achieved by adding environment cobalt (II) chloride. Concentrations of growth factors were analyzed with Bio-Plex Pro™ Assay.
  
  
  RESULTS
  ALA-PDT amplified the secretion of GM-CSF by both cell lines. The decrease in secretion of G-CSF and FGF was noticed in the SW620. SW620 line cells secreted higher levels of FGF and G-CSF, while SW480 cells more actively released GM-CSF. Compared to normoxic condition, no differences in the secretion of these factors in a hypoxic-like environment were found.
  
  
  CONCLUSIONS
  ALA-PDT increased GM-CSF secretion, which stimulates antitumor defense and decreased secretion of FGF and G-CSF-factors responsible for tumor progression. No differences in the effects of ALA-PDT in the hypoxic-like environment suggests that the beneficial results of PDT are also continued in the later stages of the reaction.
authors.affiliation: Department of Internal Diseases, 11 Listopada 48, 28-200, Staszow, Poland. Electronic address: Logia_k@wp.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wawrzyniec
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department and Clinic of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy in Bytom, Batorego 15, 41-902 Bytom, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kawczyk-Krupka
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Zenon P
authors.initials: ZP
authors.lastname: Czuba
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Król
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Department and Clinic of Internal Diseases, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy in Bytom, Batorego 15, 41-902 Bytom, Poland.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Sieroń
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000693:Anaerobiosis; D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D003035:Cobalt / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors; D016179:Granulocyte Colony-Stimulating Factor / Q000037:antagonists & inhibitors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D000860:Hypoxia; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D017319:Photosensitizing Agents; D016179:Granulocyte Colony-Stimulating Factor; D003035:Cobalt; D005346:Fibroblast Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000622:Aminolevulinic Acid; C018021:cobaltous chloride
keywords: 5-Aminolevulinic acid; Colon cancer; FGF; G-CSF; GM-CSF; Photodynamic therapy