title: Endogenous Murine BST-2/Tetherin Is Not a Major Restriction Factor of Influenza A Virus Infection.
abstract: BST-2 (tetherin, CD317, HM1.24) restricts virus growth by tethering enveloped viruses to the cell surface. The role of BST-2 during influenza A virus infection (IAV) is controversial. Here, we assessed the capacity of endogenous BST-2 to restrict IAV in primary murine cells. IAV infection increased BST-2 surface expression by primary macrophages, but not alveolar epithelial cells (AEC). BST-2-deficient AEC and macrophages displayed no difference in susceptibility to IAV infection relative to wild type cells. Furthermore, BST-2 played little role in infectious IAV release from either AEC or macrophages. To examine BST-2 during IAV infection in vivo, we infected BST-2-deficient mice. No difference in weight loss or in viral loads in the lungs and/or nasal tissues were detected between BST-2-deficient and wild type animals. This study rules out a major role for endogenous BST-2 in modulating IAV in the mouse model of infection.
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Londrigan
authors.affiliation: Centre for Innate Immunity and Infectious Diseases, MIMR-PHI Institute of Medical Research, Clayton, Victoria, 3168, Australia.|Monash University, Clayton, Victoria, 3168, Australia.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Tate
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Emma R
authors.initials: ER
authors.lastname: Job
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Moffat
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Wakim
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Gonelli
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Damien F J
authors.initials: DF
authors.lastname: Purcell
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Brooks
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.|Department of Biochemistry and Molecular Biology, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Rd, Parkville, Victoria, 3010, Australia.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.affiliation: Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, Victoria, 3010, Australia.|WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, 3000, Australia.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Reading
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Melbourne, Bio21 Molecular Science and Biotechnology Institute, 30 Flemington Rd, Parkville, Victoria, 3010, Australia.
authors.forename: Justine D
authors.initials: JD
authors.lastname: Mintern
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D002460:Cell Line; D004195:Disease Models, Animal; D004285:Dogs; D004847:Epithelial Cells / Q000821:virology*; D005260:Female; D005786:Gene Expression Regulation; D009980:Influenza A virus; D008168:Lung / Q000821:virology; D008264:Macrophages / Q000166:cytology / Q000821:virology*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000166:cytology; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D008562:Membrane Glycoproteins; C513868:BST2 protein, mouse; D000074010:Bone Marrow Stromal Antigen 2