title: Recombination elevates the effective evolutionary rate and facilitates the establishment of HIV-1 infection in infants after mother-to-child transmission.
abstract: BACKGROUND
  Previous studies have demonstrated that single HIV-1 genotypes are commonly transmitted from mother to child, but such analyses primarily used single samples from mother and child. It is possible that in a single sample, obtained early after infection, only the most replication competent virus is detected even when other forms may have been transmitted. Such forms may have advantages later in infection, and may thus be detected in follow-up samples. Because HIV-1 frequently recombines, phylogenetic analyses that ignore recombination may miss transmission of multiple forms if they recombine after transmission. Moreover, recombination may facilitate adaptation, thus providing an advantage in establishing infection. The effect of recombination on viral evolution in HIV-1 infected children has not been well defined.
  
  
  RESULTS
  We analyzed full-length env sequences after single genome amplification from the plasma of four subtype B HIV-1 infected women (11-67 env clones from 1 time point within a month prior to delivery) and their non-breastfed, intrapartum-infected children (3-6 longitudinal time points per child starting at the time of HIV-1 diagnosis). To address the potential beneficial or detrimental effects of recombination, we used a recently developed hierarchical recombination detection method based on the pairwise homoplasy index (PHI)-test. Recombination was observed in 9-67% of the maternal sequences and in 25-60% of the child sequences. In the child, recombination only occurred between variants that had evolved after transmission; taking recombination into account, we identified transmission of only 1 or 2 phylogenetic lineages from mother to child. Effective HIV-1 evolutionary rates of HIV-1 were initially high in the child and slowed over time (after 1000 days). Recombination was associated with elevated evolutionary rates.
  
  
  CONCLUSIONS
  Our results confirm that 1-2 variants are typically transmitted from mothers to their newborns. They also demonstrate that early abundant recombination elevates the effective evolutionary rate, suggesting that recombination increases the rate of adaptation in HIV-1 evolution.
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, 01605, MA, USA. keri.sanborn@umassmed.edu.
authors.forename: Keri B
authors.initials: KB
authors.lastname: Sanborn
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, 01605, MA, USA. mohan.somasundaran@umassmed.edu.
authors.forename: Mohan
authors.initials: M
authors.lastname: Somasundaran
authors.affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, 01605, MA, USA. katherine.luzuriaga@umassmed.edu.
authors.forename: Katherine
authors.initials: K
authors.lastname: Luzuriaga
authors.affiliation: Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA. tkl@lanl.gov.
authors.forename: Thomas
authors.initials: T
authors.lastname: Leitner
mesh_terms: D019143:Evolution, Molecular; D005260:Female; D015752:Genes, env; D014644:Genetic Variation; D016679:Genome, Viral; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D009035:Mothers; D010802:Phylogeny; D011247:Pregnancy; D011995:Recombination, Genetic