title: The effects of cold stress on the phenolic compounds and antioxidant capacity of grapevine (Vitis vinifera L.) leaves.
abstract: According to some estimates, a 70% increase in crop yield could be achieved if the environmental conditions were close to the optimum ones for a given plant, which is why the identification and control of adverse environmental effects is a top priority in many countries worldwide. This paper contains a discussion of the changes in selected elements of the secondary metabolism in the leaves of two grapevine varieties (Vitis vinifera L.) with a different degree of tolerance to cold stress during prolonged and constant low temperature stress. The analyses have shown that the more-tolerant variety was characterized by a higher content of phenolic compounds, better radical-scavenging capacity and stronger reducing power. However, the cold stress caused a decrease in the concentration of the phenolics and decreased the scavenging capacity in the leaves of both varieties. Four phenolic acids have been identified in the extracts from the leaves of both grapevines: caffeic acid, p-coumaric acid, ferulic acid and a caffeic acid derivative. Caffeic acid appeared in the highest concentrations in all the leaf extracts. Additionally, it has been noted that in the leaves of the varieties susceptible and tolerant to cold stress, the prolonged exposure to low temperature caused a considerable reduction of the content of all identified phenolic acids. The results of the analyses have demonstrated large differences in the functioning of the secondary metabolism in response to the same stressor.
authors.affiliation: Department of Biology and Biotechnology, Chair of Biochemistry, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego St. 1A, Kortowo, 10-957 Olsztyn, Poland. Electronic address: angelika.krol@uwm.edu.pl.
authors.forename: A
authors.initials: A
authors.lastname: Król
authors.affiliation: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima Street 10, 10-748 Olsztyn, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Amarowicz
authors.affiliation: Department of Biology and Biotechnology, Chair of Biochemistry, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego St. 1A, Kortowo, 10-957 Olsztyn, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Weidner
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002109:Caffeic Acids / Q000378:metabolism; D003080:Cold Temperature; D003373:Coumaric Acids / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000502:physiology*; D011422:Propionates; D064210:Secondary Metabolism; D013312:Stress, Physiological; D027843:Vitis / Q000737:chemistry / Q000502:physiology*
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010636:Phenols; D011422:Propionates; C004999:ferulic acid; C017616:phenolic acid; C495469:p-coumaric acid; C040048:caffeic acid
keywords: Abiotic stress; Antioxidants; Grapevine coultivars; Phenolic acids; Phenolic compounds