title: Evaluation of a triplex real-time PCR system to detect the plant-pathogenic molds Alternaria spp., Fusarium spp. and C. purpurea.
abstract: This article describes the development of a triplex real-time PCR system for the simultaneous detection of three major plant-pathogenic mold genera (Alternaria spp., Fusarium spp. and the species Claviceps purpurea). The designed genus-specific primer-probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA.
authors.affiliation: Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, D-41065 Mönchengladbach, Germany; Institute of Virology and Microbiology, University of Witten/Herdecke, D-58448 Witten, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Grube
authors.affiliation: GEN-IAL GmbH, D-53842 Troisdorf, Germany.
authors.forename: Jutta
authors.initials: J
authors.lastname: Schönling
authors.affiliation: Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, D-41065 Mönchengladbach, Germany; Institute of Virology and Microbiology, University of Witten/Herdecke, D-58448 Witten, Germany; Center for Advanced Microstructures and Devices (CAMD), Louisiana State University, Baton Rouge, LA 70806, United States. Electronic address: a.prange@gmx.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Prange
mesh_terms: D000528:Alternaria / Q000235:genetics / Q000302:isolation & purification*; D002967:Claviceps / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal
keywords: Alternaria spp.; C. purpurea; Fungal contamination; Fusarium spp.; Plant pathogen molds; Real-time PCR