title: Serotoninergic antidepressants positively affect platelet ADAM10 expression in patients with Alzheimer's disease.
abstract: Studies have demonstrated a decreased platelet ADAM10 expression in patients with Alzheimer's Disease (AD), classifying this protein as a blood-based AD biomarker. About 50% of the patients with AD are diagnosed with depression, which is commonly treated with tricyclic and tetracyclic antidepressants, monoaminoxidade (MAO) inhibitors and, more preferably, with selective serotonin reuptake inhibitors (SSRIs). Considering that a large proportion of patients with AD takes antidepressant medications during the course of the disease we investigated the influence of this medication on the expression of platelet ADAM10, which is considered the main α-secretase preventing beta-amyloid (βA) formation.
  
  
  
  Blood was collected for protein extraction from platelets. ADAM10 was analyzed by using western blotting and reactive bands were measured using β-actin as endogenous control.
  
  
  
  Platelet ADAM10 protein expression in patients with AD was positively influenced by serotoninergic medication.
  
  
  
  More studies on the positive effects of serotonergic antidepressants on ADAM10 platelet expression should be performed in order to understand its biological mechanisms and to verify whether these effects are reflected in the central nervous system. This work represents an important advance for the study of AD biomarkers, as well as for more effective pharmacological treatment of patients with AD and associated depression.
authors.affiliation: Department of Gerontology,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Otávio Augusto Fernandes Marques
authors.initials: OA
authors.lastname: Bianco
authors.affiliation: Department of Gerontology,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Patrícia Regina
authors.initials: PR
authors.lastname: Manzine
authors.affiliation: Department of Gerontology,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Carla Manuela Crispim
authors.initials: CM
authors.lastname: Nascimento
authors.affiliation: Department of Medicine,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Francisco Assis Carvalho
authors.initials: FA
authors.lastname: Vale
authors.affiliation: Department of Gerontology,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Sofia Cristina Iost
authors.initials: SC
authors.lastname: Pavarini
authors.affiliation: Department of Gerontology,Federal University of São Carlos (UFSCar),São Carlos,SP,Brazil.
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Cominetti
mesh_terms: D000072197:ADAM10 Protein / Q000187:drug effects / Q000378:metabolism*; D000544:Alzheimer Disease / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D053829:Amyloid Precursor Protein Secretases / Q000097:blood / Q000378:metabolism; D000928:Antidepressive Agents / Q000009:adverse effects / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001938:Brazil; D003866:Depressive Disorder / Q000188:drug therapy; D005260:Female; D006801:Humans; D017367:Selective Serotonin Reuptake Inhibitors / Q000009:adverse effects / Q000627:therapeutic use*
chemical_list: D000072197:ADAM10 Protein; D053829:Amyloid Precursor Protein Secretases; D000928:Antidepressive Agents; D015415:Biomarkers; D017367:Selective Serotonin Reuptake Inhibitors
keywords: Alzheimer’s disease; antidepressant drugs; biomarkers; dementia; depression; human ADAM10 protein