title: Two rhodamine lactam modulated lysosome-targetable fluorescence probes for sensitively and selectively monitoring subcellular organelle pH change.
abstract: Be a powerful technique for convenient detection of pH change in living cells, especially at subcellular level, fluorescent probes has attracted more and more attention. In this work, we designed and synthesized three rhodamine lactam modulated fluorescent probes RS1, RS2 and RS3, which all respond sensitively toward weak acidity (pH range 4-6) via the photophysical property in buffer solution without interference from the other metal ions, and they also show ideal pKa values and excellent reversibility. Particularly, by changing the lone pair electrons distribution of lactam-N atom with different conjugations, RS2 and RS3 exhibit high quantum yield, negligible cytotoxicity and excellent permeability. They are suitable to stain selectively lysosomes of tumor cells and monitor its pH changes sensitively via optical molecular imaging. The above findings suggest that the probes we designed could act as ideal and easy method for investigating the pivotal role of H(+) in lysosomes and are potential pH detectors in disease diagnosis through direct intracellular imaging.
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: She
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Yuelu
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Jidong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China. Electronic address: liuping@nwu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China.
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China. Electronic address: lijianli@nwu.edu.cn.
authors.forename: Jianli
authors.initials: J
authors.lastname: Li
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007769:Lactams / Q000737:chemistry*; D008247:Lysosomes / Q000737:chemistry*; D015388:Organelles / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D013347:Subcellular Fractions / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D007769:Lactams; D012235:Rhodamines
keywords: Bio-imaging; Conjugation effect; Fluorescence probe; Lysosome-targetable; Rhodamine lactam