title: Bone regeneration by nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffolds seeded with human umbilical cord mesenchymal stem cells in the calvarial defects of the nude mice.
abstract: In the preliminary study, we have found an excellent osteogenic property of nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffolds seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and subcutaneously in the nude mice. The aim of this study was to further evaluate the osteogenic capacity of nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice. Totally 108 nude mice were included and divided into 6 groups: PLGA scaffolds + hUCMSCs; nHA/PLGA scaffolds + hUCMSCs; CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds without seeding; the control group (no scaffolds) (n = 18). The scaffolds were implanted into the calvarial defects of nude mice. The amount of new bones was evaluated by fluorescence labeling, H&E staining, and Van Gieson staining at 4 and 8 weeks, respectively. The results demonstrated that the amount of new bones was significantly increased in the group of nHA/CS/PLGA scaffolds seeded with hUCMSCs (p < 0.01). On the basis of previous studies in vitro and in subcutaneous implantation of the nude mice, the results revealed that the nHA and CS also enhanced the bone regeneration by nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice at early stage.
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Su
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Guo
authors.affiliation: Research Center for Stomatology, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Yin-Cheng
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Guan
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zou
authors.affiliation: Department of Orthodontics, Stomatological Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xin-Qin
authors.initials: XQ
authors.lastname: Si
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D048271:Chitosan / Q000737:chemistry; D036101:Cord Blood Stem Cell Transplantation / Q000295:instrumentation* / Q000379:methods; D017886:Durapatite / Q000737:chemistry; D019544:Equipment Failure Analysis; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000295:instrumentation*; D051379:Mice; D008819:Mice, Nude; D053761:Nanocomposites / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011474:Prosthesis Design; D012887:Skull Fractures / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; D017886:Durapatite