title: Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells.
abstract: Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors including toxins, iron acquisition systems and adhesins. In this study, a comparative proteomic approach was utilised to define the UPEC pan and core surface proteome following growth in pooled human urine. Identified proteins were investigated for subcellular origin, prevalence and homology to characterised virulence factors. Fourteen core surface proteins were identified, as well as eleven iron uptake receptor proteins and four distinct fimbrial types, including type 1, P, F1C/S and a previously uncharacterised fimbrial type, designated UCA-like (UCL) fimbriae in this study. These pathogenicity island (PAI)-associated fimbriae are related to UCA fimbriae of Proteus mirabilis, associated with UPEC and exclusively found in members of the E. coli B2 and D phylogroup. We further demonstrated that UCL fimbriae promote significant biofilm formation on abiotic surfaces and mediate specific attachment to exfoliated human uroepithelial cells. Combined, this study has defined the surface proteomic profiles and core surface proteome of UPEC during growth in human urine and identified a new type of fimbriae that may contribute to UTI.
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.
authors.forename: Daniël J
authors.initials: DJ
authors.lastname: Wurpel
authors.affiliation: Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
authors.forename: Makrina
authors.initials: M
authors.lastname: Totsika
authors.affiliation: MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, London, United Kingdom.
authors.forename: Luke P
authors.initials: LP
authors.lastname: Allsopp
authors.affiliation: Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Australia.
authors.forename: Richard I
authors.initials: RI
authors.lastname: Webb
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia.
authors.forename: Danilo G
authors.initials: DG
authors.lastname: Moriel
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Australia. Electronic address: m.schembri@uq.edu.au.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Schembri
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000378:metabolism; D036781:Fimbriae Proteins / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006801:Humans; D020543:Proteome / Q000378:metabolism; D014556:Urine / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000378:metabolism*; D019459:Urothelium / Q000382:microbiology*
chemical_list: D015815:Cell Adhesion Molecules; D029968:Escherichia coli Proteins; D020543:Proteome; D036781:Fimbriae Proteins
keywords: Adhesion; Chaperone-usher; F17-like; Outer membrane; Pili; Proteome; UPEC; Vesicles