title: Effect of α-Allocryptopine on Delayed Afterdepolarizations and Triggered Activities in Mice Cardiomyocytes Treated with Isoproterenol.
abstract: Objective. To investigate the effect of α-allocryptopine (ALL) on delayed afterdepolarization (DAD) incidence and triggered activity (TA) in mice administered isoproterenol (ISO). Methods. Mouse ventricular myocytes were isolated. And the cellular electrophysiological properties of ventricular myocytes were investigated. Results. We found that the incidences of DADs and TA in mouse myocytes were increased by ISO treatment. In sharp contrast, triggered arrhythmia events were rarely observed in myocytes with 10 μM ALL treatment. Transient inward current (I ti) was reduced significantly with ALL treatment, which contributed to DAD-related triggered arrhythmia. Compared to Iso-treated group, the L-type calcium current (I Ca,L) densities were decreased after exposure to ALL, along with slower activation, quicker inactivation, and longer time constant of recovery from inactivation kinetics. Conclusion. There is less triggered arrhythmia events in ventricular myocytes treated with ALL. This effect may be associated with the inhibition of I ti and I Ca,L.
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Lin
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Zhongqi
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Gerontology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Cardiology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li