title: High Expression of Neuropilin-1 Associates with Unfavorable Clinicopathological Features in Hepatocellular Carcinoma.
abstract: As a co-receptor for vascular endothelial growth factor (VEGF), Neuropilin-1 (NRP-1) plays an important role in angiogenesis and malignant progression of many human cancers. However, the role of NRP-1 in hepatocellular carcinoma (HCC) is not well understood. The study aimed to detected the expression of Neuropilin-1 in HCC and investigate the association between its expression and the clinicopathological characteristics and prognosis of HCC. Quantitative real-time PCR (qRT-PCR), Western blot, Immunofluorescence and immunohistochemistry (IHC) analyses were performed to characterize the expression of NRP-1 in HCC cell lines and tissues. The association of NRP-1 expression with the clinicopathological characteristics and the prognosis was subsequently assessed. qRT-PCR and Western blot assays revealed that the expression of NRP-1 in HCC was significantly increased relative to that of normal live cells and tissues (P < 0.05,and <0.001, respectively). In addition, high expression of NRP-1 was significantly associated with intrahepatic metastasis (P = 0.036), Edmondson grade (P = 0.007), TNM classification (P = 0.0031), and portal vein invasion (P = 0.004). Furthermore, the HCC patients with high NRP-1 expression had shorter overall survival (OS), and recurrence-free survival (RFS), whereas, patients with low NRP-1 expression had better OS and RFS (P = 0.0035, and 0.0048, respectively). These data indicate that NRP-1 expression may play an important role in the progression of HCC, and that high NRP-1 expression suggests unfavorable clinicopathological characteristics and survival in HCC patients.
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Nuclear Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, No.88 Jiefang Road, Hangzhou, 310009, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dou
authors.affiliation: Cancer Research Center of Medical School, Xiamen University, Xiangan South Road, Xiamen, 361102, China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Weixing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Su
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Nuclear Medicine, Zhongshan Hospital Xiamen University, No.201 Hubin South Road, Xiamen, 361004, China. suxinhuii@163.com.
authors.forename: Xinhui
authors.identifier: http://orcid.org/0000-0003-4355-6052
authors.initials: X
authors.lastname: Su
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000556:secondary* / Q000601:surgery; D016022:Case-Control Studies; D005260:Female; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D039942:Neuropilin-1 / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D039942:Neuropilin-1
keywords: Clinicopathology; Expression; Hepatocellular carcinoma; Neuropilin-1