title: A novel resource for studying function and dysfunction of α-synuclein: mouse lines for modulation of endogenous Snca gene expression.
abstract: Pathological modification of α-synuclein is believed to be an important event in pathogenesis of Parkinson's disease and several other neurodegenerative diseases. In normal cells this protein has been linked to many intracellular processes and pathways. However, neither normal function of α-synuclein in neuronal and certain other types of cells nor its exact role in the disease pathogenesis is well understood, which is largely due to limitations of animal models used for studying this protein. We produced and validated several novel mouse lines for manipulating expression of the endogenous Snca gene coding for α-synuclein. These include a line for conditional Cre-recombinase-driven inactivation of the gene; a line for conditional Flp-driven restoration of a neo-cassete-blocked α-synuclein expression; a new line with a "clean" constituent knockout of the gene as well as a line carrying this knockout locus and Rosa26-stop-lacZ reporter locus linked at the same mouse chromosome 6. Altogether these lines represent a set of new useful tools for studies of α-synuclein normal function and the role of this protein in disease pathogenesis.
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.|Institute of Physiologically Active Compounds Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka 142432, Moscow Region, Russian Federation.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ninkina
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
authors.forename: Natalie
authors.initials: N
authors.lastname: Connor-Robson
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.|Institute of Physiologically Active Compounds Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka 142432, Moscow Region, Russian Federation.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Ustyugov
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.|Institute of Physiologically Active Compounds Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka 142432, Moscow Region, Russian Federation.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Tarasova
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.|Institute of Physiologically Active Compounds Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka 142432, Moscow Region, Russian Federation.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Shelkovnikova
authors.affiliation: School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, United Kingdom.|Institute of Physiologically Active Compounds Russian Academy of Sciences, 1 Severniy proezd, Chernogolovka 142432, Moscow Region, Russian Federation.
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Buchman
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000378:metabolism*; D015870:Gene Expression; D018390:Gene Targeting / Q000379:methods; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D051844:alpha-Synuclein / Q000235:genetics* / Q000378:metabolism*
chemical_list: C497605:Snca protein, mouse; D051844:alpha-Synuclein; C045073:Cre recombinase; D019426:Integrases