title: Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes.
abstract: High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5-1.1 folds, with concomitant reduction of linoleic acid by 0.4-1.0 folds and palmitic acid by 0.1-0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53-58%), and 28 ILs with low oil content (42-50%) were selected that may be released for cultivation upon further evaluation.
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Pasupuleti
authors.initials: P
authors.lastname: Janila
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Manish K
authors.initials: MK
authors.lastname: Pandey
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Yaduru
authors.initials: Y
authors.lastname: Shasidhar
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Murali T
authors.initials: MT
authors.lastname: Variath
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Manda
authors.initials: M
authors.lastname: Sriswathi
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Pawan
authors.initials: P
authors.lastname: Khera
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Surendra S
authors.initials: SS
authors.lastname: Manohar
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Patne
authors.initials: P
authors.lastname: Nagesh
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India.
authors.forename: Manish K
authors.initials: MK
authors.lastname: Vishwakarma
authors.affiliation: Directorate of Groundnut Research (DGR) of Indian Council of Agricultural Research (ICAR), Junagadh, India.
authors.forename: Gyan P
authors.initials: GP
authors.lastname: Mishra
authors.affiliation: Directorate of Groundnut Research (DGR) of Indian Council of Agricultural Research (ICAR), Junagadh, India.
authors.forename: T
authors.initials: T
authors.lastname: Radhakrishnan
authors.affiliation: Department of Oilseeds, Centre of Plant Breeding and Genetics, Tamil Nadu Agricultural University (TNAU), Coimbatore, India.
authors.forename: N
authors.initials: N
authors.lastname: Manivannan
authors.affiliation: Main Oilseeds Research Station, Junagadh Agricultural University (JAU), Junagadh, India.
authors.forename: K L
authors.initials: KL
authors.lastname: Dobariya
authors.affiliation: Regional Agricultural Research Station, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Vasanthi
authors.affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India. Electronic address: r.k.varshney@cgiar.org.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Varshney
mesh_terms: D000483:Alleles; D010367:Arachis / Q000235:genetics* / Q000378:metabolism; D003433:Crosses, Genetic; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D005819:Genetic Markers; D005838:Genotype; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008041:Linoleic Acids / Q000378:metabolism / Q000592:standards; D009154:Mutation; D009829:Oleic Acids / Q000378:metabolism / Q000592:standards; D010169:Palmitic Acids / Q000378:metabolism / Q000592:standards; D000074241:Peanut Oil; D000069600:Plant Breeding / Q000379:methods*; D010938:Plant Oils / Q000378:metabolism / Q000592:standards*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011786:Quality Control; D000068618:Selective Breeding
chemical_list: D005819:Genetic Markers; D007527:Isoenzymes; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D000074241:Peanut Oil; D010938:Plant Oils; D010940:Plant Proteins; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase
keywords: Marker-assisted backcrossing; Marker-assisted selection; Oil content; Oil quality; Oleic acid; Peanut