title: Quantitative MS analysis of therapeutic mAbs and their glycosylation for pharmacokinetics study.
abstract: Therapeutic mAbs play an important role in the treatment of a wide range of diseases. Due to their complexity, comprehensive evaluation of their pharmacokinetics has yet to be fully achieved. It is crucial to develop sensitive, accurate, reliable, and reproducible methods for quantitation of mAbs in complex samples. In addition, it is also important to evaluate the PTMs which can affect their safety and/or effectiveness. MS-based methods provide an emerging approach for quantitation of proteins and their modification forms. In this review, we give a brief overview of quantification analysis of mAbs in complex biological samples and the characterization of N-glycosylation by biological MS.
authors.affiliation: Research Center for Drug Metabolism, School of Life Sciences, Jilin University, Changchun, China.|CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cong
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Research Center for Drug Metabolism, School of Life Sciences, Jilin University, Changchun, China.
authors.forename: Lianghai
authors.initials: L
authors.lastname: Hu
authors.affiliation: Research Center for Drug Metabolism, School of Life Sciences, Jilin University, Changchun, China.
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002853:Chromatography, Liquid; D006031:Glycosylation; D006207:Half-Life; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000032:analysis*; D007141:Immunoglobulin Fc Fragments / Q000032:analysis*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D010446:Peptide Fragments / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D059748:Proteolysis; D014357:Trypsin / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D010446:Peptide Fragments; D011134:Polysaccharides; D014357:Trypsin
keywords: Mass spectrometry; Pharmacokinetics; Proteomics; Therapeutic mAb