title: Optimization of selenylation modification for garlic polysaccharide based on immune-enhancing activity.
abstract: Garlic polysaccharide (GPS) was modified in selenylation respectively by nitric acid-sodium selenite (NA-SS), glacial acetic acid-selenous acid (GA-SA), glacial acetic acid-sodium selenite (GA-SS) and selenium oxychloride (SOC) methods each under nine modification conditions of L9(3(4)) orthogonal design and each to obtain nine selenizing GPSs (sGPSs). Their structures were identified, yields and selenium contents were determined, selenium yields were calculated, and the immune-enhancing activities of four sGPSs with higher selenium yields were compared taking unmodified GPS as control. The results showed that among four methods the selenylation efficiency of NA-SS method were the highest, the activity of sGPS5 was the strongest and significantly stronger than that of unmodified GPS. This indicates that selenylation modification can significantly enhance the immune-enhancing activity of GPS, NA-SS method is the best method and the optimal conditions are 0.8:1 weight ratio of sodium selenite to GPS, reaction temperature of 70 °C and reaction time of 10h.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: National Research Center of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shulei
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Youying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ranran
authors.initials: R
authors.lastname: Hou
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Yue
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
authors.forename: Hongquan
authors.initials: H
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: ylhu@njau.edu.cn.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D016207:Cytokines / Q000378:metabolism; D005737:Garlic / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D012643:Selenium / Q000737:chemistry*
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides; D012643:Selenium
keywords: Anhydrous sodium carbonate (PubChem CID: 10340); Ascorbic acid (PubChem CID: 54670067); Cytokines; Garlic polysaccharide; Glacial acetic acid (PubChem CID: 176); Hydrochloric acid (PubChem CID: 313); Immune response; Lymphocyte proliferation; Nitric acid (PubChem CID: 944); Perchloric acid (PubChem CID: 24247); Pyridine (PubChem CID: 1049); Selenium oxychloride (PubChem CID: 24647); Selenous acid (PubChem CID: 1091); Selenylation modification; Sodium Selenite (PubChem CID: 24934)