title: Natural Inhibitors of Lipase: Examining Lipolysis in a Single Droplet.
abstract: Inhibition of lipase activity is one of the approaches to reduced fat intake with nutritional prevention promoting healthier diet. The food industry is very interested in the use of natural extracts, hence reducing the side effects of commercial drugs inhibiting lipolysis. In this work we propose a novel methodology to rapidly assess lipolysis/inhibition in a single droplet by interfacial tension and dilatational elasticity. The evolution of the interfacial tension of lipase in simplified duodenal fluid in the absence and that in the presence of the pharmaceutical drug Xenical are the negative (5 ± 1 mN/m) and positive (9 ± 1 mN/m) controls of the inhibition of lipolysis, respectively. Then, we correlate the inhibition with the reduction of the interfacial activity of lipase and further identify the mode of action of the inhibition based on dilatational response (conformational changes induced in the molecule/blocking of adsorption sites). This work provides new insight into the lipase inhibition mechanism and a rapid methodology to identify the potential of new natural inhibitors.
authors.affiliation: Department of Applied Physics, University of Granada , Campus de Fuentenueva sn, 18071 Granada, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: del Castillo-Santaella
authors.affiliation: Department of Applied Physics, University of Granada , Campus de Fuentenueva sn, 18071 Granada, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Maldonado-Valderrama
authors.affiliation: Department of Applied Physics, University of Granada , Campus de Fuentenueva sn, 18071 Granada, Spain.
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Cabrerizo-Vílchez
authors.affiliation: Department of Biotechnology, Biosearch Life S.A. , Camino de Purchil 66, 18004 Granada, Spain.
authors.forename: Ceferino
authors.initials: C
authors.lastname: Rivadeneira-Ruiz
authors.affiliation: Department of Biotechnology, Biosearch Life S.A. , Camino de Purchil 66, 18004 Granada, Spain.
authors.forename: Deyanira
authors.initials: D
authors.lastname: Rondón-Rodriguez
authors.affiliation: Department of Applied Physics, University of Granada , Campus de Fuentenueva sn, 18071 Granada, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gálvez-Ruiz
mesh_terms: D000327:Adsorption; D004791:Enzyme Inhibitors / Q000737:chemistry*; D008049:Lipase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D008066:Lipolysis
chemical_list: D004791:Enzyme Inhibitors; D008049:Lipase
keywords: adsorption; interfacial tension; lipase; lipolysis; natural inhibitor