title: Design of a microfluidic device to quantify dynamic intra-nuclear deformation during cell migration through confining environments.
abstract: The ability of cells to migrate through tissues and interstitial spaces is an essential factor during development and tissue homeostasis, immune cell mobility, and in various human diseases. Deformation of the nucleus and its associated lamina during 3-D migration is gathering increasing interest in the context of cancer metastasis, with the underlying hypothesis that a softer nucleus, resulting from reduced levels of lamin A/C, may aid tumour spreading. However, current methods to study the migration of cells in confining three dimensional (3-D) environments are limited by their imprecise control over the confinement, physiological relevance, and/or compatibility with high resolution imaging techniques. We describe the design of a polydimethylsiloxane (PDMS) microfluidic device composed of channels with precisely-defined constrictions mimicking physiological environments that enable high resolution imaging of live and fixed cells. The device promotes easy cell loading and rapid, yet long-lasting (>24 hours) chemotactic gradient formation without the need for continuous perfusion. Using this device, we obtained detailed, quantitative measurements of dynamic nuclear deformation as cells migrate through tight spaces, revealing distinct phases of nuclear translocation through the constriction, buckling of the nuclear lamina, and severe intranuclear strain. Furthermore, we found that lamin A/C-deficient cells exhibited increased and more plastic nuclear deformations compared to wild-type cells but only minimal changes in nuclear volume, implying that low lamin A/C levels facilitate migration through constrictions by increasing nuclear deformability rather than compressibility. The integration of our migration devices with high resolution time-lapse imaging provides a powerful new approach to study intracellular mechanics and dynamics in a variety of physiologically-relevant applications, ranging from cancer cell invasion to immune cell recruitment.
authors.affiliation: Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Davidson
authors.forename: Josiah
authors.initials: J
authors.lastname: Sliz
authors.forename: Philipp
authors.initials: P
authors.lastname: Isermann
authors.forename: Celine
authors.initials: C
authors.lastname: Denais
authors.forename: Jan
authors.initials: J
authors.lastname: Lammerding
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D053144:Cell Nucleus Shape / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D057809:HEK293 Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D056656:Lab-On-A-Chip Devices; D034904:Lamin Type A / Q000172:deficiency / Q000502:physiology; D051379:Mice; D041681:NIH 3T3 Cells; D059008:Time-Lapse Imaging
chemical_list: D004129:Dimethylpolysiloxanes; D034904:Lamin Type A; C013830:baysilon