title: Characterization and immunoenhancement activities of Eucommia ulmoides polysaccharides.
abstract: The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv. polysaccharide. The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da. The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively. In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation. Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production. In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation. Together, these results suggest that EUPS is a strong immunostimulant.
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China. Electronic address: fenghaiborc@126.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan 610051, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Song
authors.affiliation: Applied Biophysics and Immune Engineering Laboratory, College of Life and Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Jishuang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Song
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects; D005260:Female; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D019684:Magnoliopsida / Q000737:chemistry; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000914:Antibodies, Viral; D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Adjuvant; Cytokines; Dendritic cell; Eucommia ulmoides Oliv. polysaccharide; Lymphocyte proliferation