title: A whole new ball game: Stem cell-derived epithelia in the study of host-microbe interactions.
abstract: Recent advances in developmental and stem cell biology have resulted in techniques that enable the generation and maintenance of complex epithelium in vitro. While these models have been utilized to study host development and disease, a renewed appreciation of host-microbe interactions has sparked interest in employing these new techniques to study microbes at the epithelial interface. Here we review the current advances in host-microbe interactions that have resulted from experiments using these complex epithelia. Furthermore we highlight aspects of these techniques that warrant further development to facilitate the study of host-microbe interactions.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr., Ann Arbor, MI 48109, USA.
authors.forename: Jhansi L
authors.initials: JL
authors.lastname: Leslie
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Dr., Ann Arbor, MI 48109, USA; Division of Infectious Diseases, Department of Internal Medicine, University of Michigan Medical School, 1150 W Medical Center Dr., Ann Arbor, MI 48109, USA. Electronic address: youngvi@umich.edu.
authors.forename: Vincent B
authors.initials: VB
authors.lastname: Young
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D016360:Clostridioides difficile / Q000502:physiology; D016480:Helicobacter pylori / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000382:microbiology*; D019779:Salmonella enterica / Q000502:physiology; D013234:Stem Cells / Q000382:microbiology
keywords: Clostridium difficile; Enteroid; Helicobacter pylori; Organoid; Salmonella enterica