title: Molecular epidemiology of genogroup II norovirus infection among hospitalized children with acute gastroenteritis in Suzhou (Jiangsu, China) from 2010 to 2013.
abstract: Noroviruses (NoVs) are the most common cause of acute gastroenteritis in both sporadic and outbreak cases. Genotyping and recombination analyses were performed in order to help getting more knowledge of the distribution and genetic diversity of NoVs in Suzhou, located in Jiangsu province of China. All stool samples were collected from hospitalized children younger than 5 years old with acute gastroenteritis. For genotyping, the open reading frame (ORF) 1 and ORF2 were partially amplified and sequenced. 26.9% of stool samples were positive for genogroup II NoVs. The most common genotype was GII.4 and its variants included Den Haag-2006b, New Orleans-2009, and Sydney-2012. The Den Haag-2006b variants predominated during 2010-2012. In 2013, it was replaced by the Sydney-2012 variant. The second most common genotype was GII.12/GII.3. NoVs could be detected throughout the year, with GII.4 and GII.12/GII.3 coexisting during the cold months, and GII.4 was the main genotype during the warm months. The highest prevalence of NoV was detected in young children aged <24 months. Patients infected with GII.4 had a higher chance of getting moderate fever than other NoV-positive patients, while those infected with GII.12/GII.3 tended to have a mild degree of fever. NoV is an important pathogen responsible for viral gastroenteritis among children in Suzhou. Analyses of NoV circulating between 2010 and 2013 revealed a change of predominant variant of NoV GII.4 in each epidemic season and intergenotype recombinant strains represented an important part.
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Jian-Guang
authors.initials: JG
authors.lastname: Fu
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ai
authors.affiliation: Suzhou Center for Disease Control and Prevention, Suzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Soochow University Affiliated Children's Hospital, Suzhou, China.
authors.forename: Qing-Bin
authors.initials: QB
authors.lastname: Wu
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Qi
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ji
authors.affiliation: Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: Suzhou Center for Disease Control and Prevention, Suzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Shen-Jiao
authors.initials: SJ
authors.lastname: Wang
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Shan
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Bao
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.
authors.forename: Fen-Yang
authors.initials: FY
authors.lastname: Tang
authors.affiliation: Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China.|The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Zhu
mesh_terms: D000208:Acute Disease; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D058872:Epidemics; D062665:Epidemiological Monitoring; D005243:Feces / Q000821:virology; D005260:Female; D005334:Fever / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D014644:Genetic Variation; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012621:Seasons; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral
keywords: genogroup II; molecular epidemiology; norovirus