title: In Vitro and In Vivo Imaging of Fluorescent Aptamers.
abstract: Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).
authors.affiliation: CEA, I2BM, Molecular Imaging Research Center (MIRCen), UMR 9199, 18 Route du Panorama, BP n°6, Fontenay-aux-Roses, 92265, France.|INSERM U1023, Paris, France.|Université Paris Sud, Paris, France.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Théodorou
authors.affiliation: CEA, I2BM, Molecular Imaging Research Center (MIRCen), UMR 9199, 18 Route du Panorama, BP n°6, Fontenay-aux-Roses, 92265, France.|INSERM U1023, Paris, France.|Université Paris Sud, Paris, France.
authors.forename: Nam Nguyen
authors.initials: NN
authors.lastname: Quang
authors.affiliation: INSERM U1023, Paris, France.|Université Paris Sud, Paris, France.|CEA, I2BM, Service Hospitalier Frédéric Joliot (SHFJ), Paris, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Gombert
authors.affiliation: INSERM U1023, Paris, France.|Université Paris Sud, Paris, France.|CEA, I2BM, Service Hospitalier Frédéric Joliot (SHFJ), Paris, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Thézé
authors.affiliation: CEA, I2BM, Molecular Imaging Research Center (MIRCen), UMR 9199, 18 Route du Panorama, BP n°6, Fontenay-aux-Roses, 92265, France.|INSERM U1023, Paris, France.|Université Paris Sud, Paris, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Lelandais
authors.affiliation: CEA, I2BM, Molecular Imaging Research Center (MIRCen), UMR 9199, 18 Route du Panorama, BP n°6, Fontenay-aux-Roses, 92265, France. frederic.duconge@cea.fr.|INSERM U1023, Paris, France. frederic.duconge@cea.fr.|Université Paris Sud, Paris, France. frederic.duconge@cea.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Ducongé
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005456:Fluorescent Dyes / Q000737:chemistry; D064593:Heterografts; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D018715:Microscopy, Video / Q000379:methods; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism; D013194:Staining and Labeling; D059008:Time-Lapse Imaging / Q000379:methods; D041622:Tomography, Optical / Q000379:methods
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes
keywords: Aptamer; Fluorescence; Fluorescence diffuse optical tomography; Oligonucleotide; Time-lapse microscopy