title: Effect of Nisin and Thermal Treatments on the Heat Resistance of Clostridium sporogenes Spores.
abstract: The aim of this research was to evaluate the effect of thermal treatments (isothermal or nonisothermal) combined with nisin, a natural antimicrobial, on the survival and recovery of Clostridium sporogenes spores. The addition of nisin to the heating medium at concentrations up to 0.1 mg liter(-1) did not reduce the heat resistance of C. sporogenes. Without a thermal treatment, nisin added at concentrations up to 0.1 mg liter(-1) did not reduce the viable counts of C. sporogenes when added to the recovery medium, but inactivation of more than 4 log cycles was achieved after only 3 s at 100°C. At 100°C, the time needed to reduce viable counts by more than 3 log cycles was nine times shorter when 0.01 mg liter(-1) nisin was added to the recovery medium than without it. The heat resistance values calculated under isothermal conditions were used to predict the survival in the nonisothermal experiments, and the predicted values accurately fit the experimental data. The combination of nisin with a thermal treatment can help control C. sporogenes.
authors.affiliation: Departamento Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional Campus Mare Nostrum, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Ros-Chumillas
authors.affiliation: Departamento Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional Campus Mare Nostrum, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena, Spain.
authors.forename: Maria-Dolores
authors.initials: MD
authors.lastname: Esteban
authors.affiliation: Departamento Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional Campus Mare Nostrum, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena, Spain.
authors.forename: Juan-Pablo
authors.initials: JP
authors.lastname: Huertas
authors.affiliation: Departamento Ingeniería de Alimentos y del Equipamiento Agrícola, Campus de Excelencia Internacional Regional Campus Mare Nostrum, Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203 Cartagena, Spain. alfredo.palop@upct.es.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Palop
mesh_terms: D000900:Anti-Bacterial Agents; D003013:Clostridium / Q000187:drug effects / Q000502:physiology*; D005520:Food Preservatives; D006358:Hot Temperature; D009561:Nisin / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D005520:Food Preservatives; D009561:Nisin