title: Capillary electrophoresis with end-column electrochemiluminescence for ultrasensitive determination of urapidil hydrochloride in rat plasma and its application to pharmacokinetics study.
abstract: A simple, sensitive and selective method for determination of urapidil hydrochloride was developed using capillary electrophoresis with electrochemiluminescence (CE-ECL) technique for the first time. Under the optimized experimental conditions, the ECL intensity was linear with the concentration of urapidil hydrochloride in the range from 0.050 to 50.0ng/mL and the detection limit was 0.014ng/mL (S/N=3). The proposed method was used for studying pharmacokinetics of urapidil hydrochloride in rat plasma and the main pharmacokinetic parameters of the peak concentration (Cmax), half life time (T1/2) and peak concentration time (Tmax) were 240.45±21.15ng/mL, 0.58±0.16h and 1.08±0.13h, respectively. The recoveries of urapidil hydrochloride in the diluted extracts of rat plasma samples ranged from 96.68 to 98.82%. The RSD was lower than 3%.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China; Shaoyang medical college, Shaoyan 422000, China.
authors.forename: Shuangjiao
authors.initials: S
authors.lastname: Sun
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
authors.forename: Chanjuan
authors.initials: C
authors.lastname: Long
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China. Electronic address: dengby16@163.com.
authors.forename: Biyang
authors.initials: B
authors.lastname: Deng
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D009942:Organometallic Compounds; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D009942:Organometallic Compounds; D010879:Piperazines; C103599:bis(bipyridyl)ruthenium(II); D015082:2,2'-Dipyridyl; C015568:urapidil
keywords: Capillary electrophoresis; Electrochemiluminescence; Pharmacokinetics; Rat plasma; Urapidil hydrochloride