title: Biological characteristics of different epidemic enterovirus 71 strains and their pathogeneses in neonatal mice and rhesus monkeys.
abstract: Hand, foot and mouth disease (HFMD) has been prevalent in China since 2008. Enterovirus 71 (EV71) is a common causative agent of HFMD, and various strains of EV71 are prevalent worldwide. The EV71C4 subgenotype is the most endemic strain in China. However, few studies investigating the biological characteristics and pathogeneses of different C4 strains have been reported. Therefore, the current study investigated 19 clinical EV71 strains in neonatal ICR mice and neonatal rhesus monkeys by comparing pathogenicity; the virulence of different viral passages, dosages, and routes of infection; and the effects produced by subject animal age. These 19 clinical EV71 strains, which were of the same subtype, displayed varying pathogenic effects. Three strains (HE31, 231 and 262) induced limb paralysis in neonatal ICR mice. In addition, the degree of virulence was largely dependent upon the dose, route of infection, and number of passages of the challenge virus, as well as the ages of the infected animals. The present study provides valuable basic data to enable further research into EV71 pathogenesis and to facilitate the development of new drugs and vaccines.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Zhongping
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Li
authors.initials: L
authors.lastname: Hua
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ting
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Zhengling
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Min
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Xie
authors.initials: X
authors.lastname: Tianhong
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Long
authors.initials: L
authors.lastname: Runxiang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Dong
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Guangju
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Rong
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Fangyu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China. Electronic address: imbcams.lq@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Qihan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002681:China; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology*; D058872:Epidemics; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D008253:Macaca mulatta; D008813:Mice, Inbred ICR; D014774:Virulence
keywords: Animal models; Enterovirus 71 (EV71); Foot and mouth disease (HFMD); Hand