title: Application and optimization of organic-inorganic hybrid monolithic capillary electrochromatography for in vivo cefdinir determination with microdialysis.
abstract: In this study, an organic-inorganic hybrid monolithic capillary column was applied and optimized for the determination of cefdinir in plasma, and the electro-osmotic flow that usually hinders migration in reverse polarity became a driving force. The Sample used for pharmacokinetic research was collected by microdialysis using phosphate buffer (pH 7.4) as perfusate, and a volume of 60 μL fluid was mixed with 140 μL of acetonitrile. By using a silica-allyldimethyldodecylammonium monolithic column (100 μm inner diameter, 21 cm effective length and 31.2 cm total length), and a mobile phase consisting of phosphate and acetonitrile (pH 4.5, 50:50, v/v), at a voltage of 20 kV, the analytes were successfully separated with the background within 2.5 min. The detection wavelength was 214 nm. The calibration curve showed a good linearity (r(2) = 0.9994) over the concentration range of 0.2-50 μg/mL. The proposed method showed good specificity, linearity, sensitivity, precision and recovery, and the introduction of field amplified sample stacking helped to improve the low recovery caused by microdialysis. This method was successfully applied to quantify cefdinir in rat plasma to support a pre-clinical pharmacokinetic trial.
authors.affiliation: Department of TCM analysis, Guangdong Province Engineering Technology Research Institute of TCM, Guangzhou, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of TCM analysis, Guangdong Province Engineering Technology Research Institute of TCM, Guangzhou, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Bi
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, PR China.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, PR China.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, PR China.
authors.forename: Yuanjun
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai, PR China.|Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Shanghai, PR China.
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.affiliation: Department of TCM analysis, Guangdong Province Engineering Technology Research Institute of TCM, Guangzhou, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Sun
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000493:pharmacokinetics*; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D000077525:Cefdinir; D002511:Cephalosporins / Q000302:isolation & purification / Q000493:pharmacokinetics*; D017551:Microdialysis; D051381:Rats
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000077525:Cefdinir
keywords: Capillary electrochromatography; Cefdinir; Hybrid monolithic columns; Microdialysis