title: [Genetic diversity analysis of Fusarium oxysporum f. sp. cubense populations from China using Inter-Simple Sequence Repeats-PCR (ISSR-PCR) technique].
abstract: OBJECTIVE
  We used Inter-Simple Sequence Repeats (ISSR) markers to reveal the genetic diversity of 95 Fusarium oxysporum f. sp. cubense ( FOC ) isolates from banana in China, for the rational control of the disease.
  
  
  METHODS
  Eight primers were chosen for analyzing FOC isolates to study their genetic diversity by ISSR-PCR. All isolates were clustered using Unweighted Pair-Group Method with Arithmetic means (UPGMA) analysis by NTSYSpc v2.10e software.
  
  
  RESULTS
  A total of 52 sites were generated, among them 92.3% were polymorphic. Genetic distance was 0.57 to 1.00 based on the Nei's standard. Isolates were grouped into six distinct clusters (A, B, C, D, E and F) based on ISSR analysis using a genetic distance threshold of 0.68, the proportion of 51.06%, 39.58%, 5.20%, 2.08%, 1.04%, and 1.04%, respectively.
  
  
  CONCLUSIONS
  There were high levels of genetic variation among the FOC isolates, and the ISSR clustering groups had obvious correlation with hosts and races of the pathogen.
authors.forename: He
authors.initials: H
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jinji
authors.initials: J
authors.lastname: Pu
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Qi
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Qunfang
authors.initials: Q
authors.lastname: Yu
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Zhang
authors.forename: Yixian
authors.initials: Y
authors.lastname: Xie
mesh_terms: D002681:China; D004271:DNA, Fungal / Q000235:genetics; D005670:Fusarium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D014644:Genetic Variation; D058507:Host Specificity; D018895:Microsatellite Repeats; D028521:Musa / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004271:DNA, Fungal