title: [Effect of the nerve growth factor mimetic GK-2 on post-resuscitation expression of neurotrophic factors].
abstract: OBJECTIVE
  to elucidate an influence of nerve growth factor mimetic GK-2 on the expression of neurotrophic factors and the process of neuronal death after ischemia-reperfusion. Materials and methods. Adult white male rats underwent cardiac arrest for 12 minutes, followed by resuscitation. 10 rats were injected GK-2 (Img/kg i/ρ) at 30 minutes and 48 hours after resuscitation. 10 untreated animals received equivalent doses of saline. The control group consisted of sham-operated animals (n = 10). On the 7th postoperative day the total density of hypoxia-sensitive cerebellar Purkinje cells was determined by morphometric analysis. Immunohistochemical study of proteins FGFb, NT4, BDNF was performed by indirect peroxidase-antiperoxidase method using primary polyclonal antibodies. The number of neurons with different expression levels of the neurotrophic factors was determined.
  
  
  RESULTS
  In the post-resuscitation period the neuronal loss was detected in untreated animals. Namely NT4-negative, FGFb-negative and BDNF-negative cells died. GK-2 had no effect on the expression level of FGFb and NT4, however, promoted an increase in the expression level of BDNF. Initiating the expression of BDNF in neurons that were not previously producing this factor, GK-2 prevents the development of post-resuscitation neuronal death. Obtained facts lead to the conclusion that one of the mechanisms of neuroprotective action of nerve growth factor mimetic GK-2 is its ability to activate the expression of BDNF in nerve cells.
authors.forename: M Sh
authors.initials: MSh
authors.lastname: Avrushchenko
authors.forename: I V
authors.initials: IV
authors.lastname: Ostrova
authors.forename: Yu V
authors.initials: YV
authors.lastname: Zarzhetsky
authors.forename: V V
authors.initials: VV
authors.lastname: Moroz
authors.forename: T A
authors.initials: TA
authors.lastname: Gudasheva
authors.forename: S B
authors.initials: SB
authors.lastname: Seredenin
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis; D004151:Dipeptides / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006323:Heart Arrest / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D020932:Nerve Growth Factor; D011689:Purkinje Cells / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012151:Resuscitation
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004151:Dipeptides; C572946:bis(N-succinyl-glutamyllysin)hexamethylenediamine; D016222:Fibroblast Growth Factor 2; D020932:Nerve Growth Factor