title: Dissociation of cephamycin C and clavulanic acid biosynthesis by 1,3-diaminopropane in Streptomyces clavuligerus.
abstract: Streptomyces clavuligerus produces simultaneously cephamycin C (CephC) and clavulanic acid (CA). Adding 1,3-diaminopropane to culture medium stimulates production of beta-lactam antibiotics. However, there are no studies on the influence of this diamine on coordinated production of CephC and CA. This study indicates that 1,3-diaminopropane can dissociate CephC and CA productions. Results indicated that low diamine concentrations (below 1.25 g l(-1)) in culture medium increased CA production by 200%, but not that of CephC. Conversely, CephC production increased by 300% when 10 g l(-1) 1,3-diaminopropane was added to culture medium. Addition of just L-lysine (18.3 g l(-1)) to culture medium increased both biocompounds. On the other hand, while L-lysine plus 7.5 g l(-1) 1,3-diaminopropane increased volumetric production of CephC by 1100%, its impact on CA production was insignificant. The combined results suggest that extracellular concentration of 1,3-diaminopropane may trigger the dissociation of CephC and CA biosynthesis in S. clavuligerus.
authors.affiliation: Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil carlaandrealeite@iq.unesp.br.
authors.forename: Carla A
authors.initials: CA
authors.lastname: Leite
authors.affiliation: Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil.
authors.forename: André P
authors.initials: AP
authors.lastname: Cavallieri
authors.affiliation: Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil.
authors.forename: Amanda S
authors.initials: AS
authors.lastname: Baptista
authors.affiliation: Department of Biochemistry and Technological Chemistry, Institute of Chemistry, UNESP-São Paulo State University, 14800-900 Araraquara, SP, Brazil.
authors.forename: Maria L G C
authors.initials: ML
authors.lastname: Araujo
mesh_terms: D053898:Biosynthetic Pathways / Q000187:drug effects*; D002513:Cephamycins / Q000096:biosynthesis*; D019818:Clavulanic Acid / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry; D003959:Diamines / Q000378:metabolism*; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D002513:Cephamycins; D003470:Culture Media; D003959:Diamines; D019818:Clavulanic Acid; C009475:trimethylenediamine; C024925:cephamycin C
keywords: L-lysine; Streptomyces clavuligerus; beta-lactam antibiotics; culture medium; diamine