title: fosI Is a New Integron-Associated Gene Cassette Encoding Reduced Susceptibility to Fosfomycin.
abstract: In this work, we demonstrate that the fosI gene encodes a predicted small protein with 134 amino acids and determines reduced susceptibility to fosfomycin. It raised the MIC from 0.125 to 6 μg/ml when the pBRA100 plasmid was introduced into Escherichia coli TOP10 and to 16 μg/ml when the gene was cloned into the pBC_SK(-) vector and expressed in E. coli TOP10.
authors.affiliation: School of Medicine, Infectious Diseases Department, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Karla de Oliveira
authors.initials: Kde O
authors.lastname: Pelegrino
authors.affiliation: Clinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Juliana Coutinho
authors.initials: JC
authors.lastname: Campos
authors.affiliation: Departamento de Microbiologia e Imunologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Suely Carlos Ferreira
authors.initials: SC
authors.lastname: Sampaio
authors.affiliation: School of Medicine, Otorhinolaryngology Lab/LIM32, Hospital das Clínicas, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Lezirovitz
authors.affiliation: Clinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Bruna Mara
authors.initials: BM
authors.lastname: Seco
authors.affiliation: Clinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Mayne de Oliveira
authors.initials: Mde O
authors.lastname: Pereira
authors.affiliation: Clinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Darlan Augusto da Costa
authors.initials: DA
authors.lastname: Rocha
authors.affiliation: Université Limoges, INSERM, CHU Limoges, UMR_S 1092, Limoges, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Jové
authors.affiliation: School of Medicine, Infectious Diseases Department, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Antonio Carlos
authors.initials: AC
authors.lastname: Nicodemo
authors.affiliation: Clinical Analysis and Toxicology Department, School of Pharmacy, University of São Paulo, São Paulo, SP, Brazil Fleury Medicine and Health, Microbiology Department, São Paulo, SP, Brazil sampaio@usp.br.
authors.forename: Jorge Luiz Mello
authors.initials: JL
authors.lastname: Sampaio
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005578:Fosfomycin / Q000494:pharmacology*; D015870:Gene Expression; D032023:Integrons; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014169:Transformation, Bacterial
chemical_list: D000900:Anti-Bacterial Agents; D005578:Fosfomycin