title: Genome Characteristics of a Novel Type I Methanotroph (Sn10-6) Isolated from a Flooded Indian Rice Field.
abstract: Flooded rice fields are important sources of atmospheric methane. Aerobic methanotrophs living in the vicinity of rice roots oxidize methane and act as environmental filters. Here, we present genome characteristics of a gammaproteobacterial methanotroph, isolate Sn10-6, which was isolated from a rice rhizosphere of a flooded field in India. Sn10-6 has been identified as a member of a putative novel genus and species within the family Methylococcaceae (Type I methanotrophs). The draft genome of Sn10-6 showed pathways for the following: methane oxidation, formaldehyde assimilation (RuMP), nitrogen fixation, conversion of nitrite to nitrous oxide, and other interesting genes including the ones responsible for survival in the rhizosphere environment. The majority of genes found in this genome were most similar to Methylovulum miyakonese which is a forest isolate. This draft genome provided insight into the physiology, ecology, and phylogeny of this gammaproteobacterial methanotroph.
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India. monalirahalkar@aripune.org.
authors.forename: Monali C
authors.identifier: http://orcid.org/0000-0003-0945-4378
authors.initials: MC
authors.lastname: Rahalkar
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India.
authors.forename: Pranitha S
authors.initials: PS
authors.lastname: Pandit
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Dhakephalkar
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India.
authors.forename: Soham
authors.initials: S
authors.lastname: Pore
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Arora
authors.affiliation: MACS Agharkar Research Institute, G.G. Agarkar Road, Pune, 411004, Maharashtra, India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Kapse
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D055868:Floods; D016680:Genome, Bacterial; D007194:India; D008697:Methane / Q000378:metabolism*; D008772:Methylococcaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012275:Oryza / Q000254:growth & development* / Q000382:microbiology; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D008697:Methane
keywords: Draft genome; Flooded rice fields; Methanotrophs