title: Sinigrin, a major glucosinolate from cruciferous vegetables restrains non-enzymatic glycation of albumin.
abstract: Sinigrin is a major component of cruciferous vegetables such as cabbage, Brussels sprout, mustard greens and broccoli. The present study demonstrates the protective effects of sinigrin against the non-enzymatic glycation of albumin and lens crystallin based on fluorescence spectroscopy, circular dichroism and molecular interaction studies. Sinigrin was found to be a potent inhibitor for both the early (Amadori product) and advanced glycation end products (AGEs). In addition, the in vitro glycation studies of lens crystallin demonstrated the strong antiglycation activity of sinigrin. Computational studies using molecular docking approach revealed the interaction pattern of sinigrin with BSA and the binding affinity of sinigrin was found to be greater than the other potent natural inhibitors of glycation such as quercetin, apigenin, and curcumin.
authors.affiliation: Molecular Biophysics Lab, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Awasthi
authors.affiliation: Molecular Biophysics Lab, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613401, Tamilnadu, India. Electronic address: saras@scbt.sastra.edu.
authors.forename: N T
authors.initials: NT
authors.lastname: Saraswathi
mesh_terms: D000818:Animals; D019607:Brassicaceae / Q000737:chemistry*; D002417:Cattle; D005961:Glucosinolates / Q000378:metabolism / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006041:Goats; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D062105:Molecular Docking Simulation; D066329:Protein Aggregates; D050050:Protein Carbonylation / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005961:Glucosinolates; D017127:Glycation End Products, Advanced; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine; C010330:sinigrin; D008239:Lysine
keywords: Advanced glycation end products; Albumin; Sinigrin