title: miR-339-5p inhibits migration and invasion in ovarian cancer cell lines by targeting NACC1 and BCL6.
abstract: This study aimed to explore the role of miR-339-5p in ovarian cancer. The expression of miR-339-5p in seven ovarian cancer cell lines (Hey, SKOV3, OVCAR5, SKOV3-IP, A2780, CAOV3, and OVCA433) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The miR-339-5p mimic and inhibitor were used to regulate its expression. Migration, invasion, and proliferation were examined. A bioinformatics analysis was used to predict targets, and a dual-luciferase reporter system was applied for validation, along with Western blot verification. Additionally, the association of miR-339-5p and its target genes with ovarian cancer was analyzed based on The Cancer Genome Atlas (TCGA) database. OVCAR5 and SKOV3 had the highest and lowest miR-339-5p expression, respectively. Inhibition of miR-339-5p expression increased the migration and invasion of OVCAR5 cells, while in SKOV3 cells, upregulated miR-339-5p attenuated the migration and invasion ability. Modulation of miR-339-5p had no effect on proliferation. The genes nucleus accumbens associated 1(BEN and BTB (POZ) domain containing) (NACC1) and B cell lymphoma-6 (bcl6) were validated to be targets of miR-339-5p. Clinically, patients with a high expression of NACC1 had a high risk in the survival analysis. miR-339-5p inhibits migration and invasion in ovarian cancer by targeting NACC1 and BCL6. miR-339-5p may be a biomarker of metastasis in ovarian cancer; NACC1 had a predictive value for ovarian cancer progression.
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 128 Shenyang Road in Yangpu District, Shanghai, 200011, China.|Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Shan
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 128 Shenyang Road in Yangpu District, Shanghai, 200011, China.|Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 128 Shenyang Road in Yangpu District, Shanghai, 200011, China.|Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 128 Shenyang Road in Yangpu District, Shanghai, 200011, China. 052108365@fudan.edu.cn.|Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, 200011, China. 052108365@fudan.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D011266:Pregnancy-Associated Plasma Protein-A / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C084103:BCL6 protein, human; D018797:Cell Cycle Proteins; C543820:MIRN339 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C516477:NACC1 protein, human; D009363:Neoplasm Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins; C490198:PTP4A1 protein, human; D017027:Protein Tyrosine Phosphatases; D011266:Pregnancy-Associated Plasma Protein-A; C579283:PAPPA protein, human
keywords: Invasion; Migration; Ovarian cancer; miR-339-5p