title: Overexpression of the olive acyl carrier protein gene (OeACP1) produces alterations in fatty acid composition of tobacco leaves.
abstract: Taking into account that fatty acid (FA) biosynthesis plays a crucial role in lipid accumulation in olive (Olea europaea L.) mesocarp, we investigated the effect of olive acyl carrier protein (ACP) on FA composition by overexpressing an olive ACP cDNA in tobacco plants. The OeACP1.1A cDNA was inserted in the nucleus or in the chloroplast DNA of different tobacco plants, resulting in extensive transcription of the transgenes. The transplastomic plants accumulated lower olive ACP levels in comparison to nuclear-transformed plants. Moreover, the phenotype of the former plants was characterized by pale green/white cotyledons with abnormal chloroplasts, delayed germination and reduced growth. We suggest that the transplastomic phenotype was likely caused by inefficient olive ACP mRNA translation in chloroplast stroma. Conversely, total lipids from leaves of nuclear transformants expressing high olive ACP levels showed a significant increase in oleic acid (18:1) and linolenic acid (18:3), and a concomitant significant reduction of hexadecadienoic acid (16:2) and hexadecatrienoic acid (16:3). This implies that in leaves of tobacco transformants, as likely in the mesocarp of olive fruit, olive ACP not only plays a general role in FA synthesis, but seems to be specifically involved in chain length regulation forwarding the elongation to C18 FAs and the subsequent desaturation to 18:1 and 18:3.
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: De Marchis
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.|PlantLab, Institute of Life Sciences, Scuola Superiore Sant'Anna, 56127, Pisa, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Valeri
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Pompa
authors.affiliation: LISM, CNRS, Aix-Marseille University, 13402, Marseille Cedex 20, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bouveret
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.|Research Unit for Table Grapes and Wine Growing in Mediterranean Environment, CREA, Via Casamassima 148, Turi, 70010, Bari, Italy.
authors.forename: Fiammetta
authors.initials: F
authors.lastname: Alagna
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Grisan
authors.affiliation: Institute for Agricultural and Forest Systems in the Mediterranean (ISAFOM), Research Division of Perugia, CNR, Via Madonna Alta 128, 06128, Perugia, Italy.
authors.forename: Vitale
authors.initials: V
authors.lastname: Stanzione
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Mariotti
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Nicolò
authors.initials: N
authors.lastname: Cultrera
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Baldoni
authors.affiliation: Institute of Biosciences and Bioresources (IBBR), Research Division of Perugia, CNR, Via Madonna Alta 130, 06128, Perugia, Italy. michele.bellucci@ibbr.cnr.it.
authors.forename: Michele
authors.identifier: http://orcid.org/0000-0001-7476-9603
authors.initials: M
authors.lastname: Bellucci
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics / Q000378:metabolism*; D018548:Cotyledon / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics; D050356:Lipid Metabolism / Q000235:genetics; D031658:Olea / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D018087:Plastids / Q000235:genetics; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
chemical_list: D000213:Acyl Carrier Protein; D005227:Fatty Acids
keywords: Chloroplast transformation; Chloroplast ultrastructure; Fatty acid; Nuclear transformation; White cotyledons