title: Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells.
abstract: Accumulating evidence implicates phosphoinositide 4-phosphate as a regulatory molecule in its own right recruiting specific effector proteins to cellular membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.
authors.affiliation: Division of Medicine, Institute for Liver & Digestive Health, Royal Free Hospital, University College London (UCL), Royal Free Campus, Rowland Hill Street, London, NW3 2PF, UK. k.rombouts@ucl.ac.uk.
authors.forename: Krista
authors.initials: K
authors.lastname: Rombouts
authors.affiliation: Department of Experimental and Clinical Medicine, Center for Research, Transfer and High Education, DENOthe, University of Florence, Largo Brambilla 3, Florence, 50134, Italy. vinicio.carloni@unifi.it.
authors.forename: Vinicio
authors.initials: V
authors.lastname: Carloni
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D055166:Hepatic Stellate Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D021962:Membrane Microdomains / Q000378:metabolism; D011485:Protein Binding; D059470:Tetraspanins / Q000378:metabolism*
chemical_list: D059470:Tetraspanins; D019870:1-Phosphatidylinositol 4-Kinase
keywords: Density gradient centrifugation; Hepatocellular carcinoma cells; Human hepatic stellate cells; Tetraspanin -enriched microdomains; Tetraspanins; Thin layer chromatography