title: Therapeutic effects of mouse bone marrow-derived clonal mesenchymal stem cells in a mouse model of inflammatory bowel disease.
abstract: Mouse bone marrow-derived clonal mesenchymal stem cells (mcMSCs), which were originated from a single cell by a subfractionation culturing method, are recognized as new paradigm for stem cell therapy featured with its homogenous cell population. Next to proven therapeutic effects against pancreatitis, in the current study we demonstrated that mcMSCs showed significant therapeutic effects in dextran sulfate sodium (DSS)-induced experimental colitis model supported with anti-inflammatory and restorative activities. mcMSCs significantly reduced the disease activity index (DAI) score, including weight loss, stool consistency, and intestinal bleeding and significantly increased survival rates. The pathological scores were also significantly improved with mcMSC. We have demonstrated that especial mucosal regeneration activity accompanied with significantly lowered level of apoptosis as beneficiary actions of mcMSCs in UC models. The levels of inflammatory cytokines including TNF-α, IFN-γ, IL-1β, IL-6, and IL-17 were all significantly concurrent with significantly repressed NF-κB activation compared to the control group and significantly decreased infiltrations of responsible macrophage and neutrophil. Conclusively, our findings provide the rationale that mcMSCs are applicable as a potential source of cell-based therapy in inflammatory bowel diseases, especially contributing either to prevent relapse or to accelerate healing as solution to unmet medical needs in IBD therapy.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746, Korea.
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Park
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea ; SCM Lifescience Co., Ltd., 366 Saohae-daero, Jung-gu, Incheon 400-711, Korea.
authors.forename: Tac-Ghee
authors.initials: TG
authors.lastname: Yi
authors.affiliation: Digestive Disease Center, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam 463-838, Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Digestive Disease Center, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam 463-838, Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Han
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746, Korea.
authors.forename: Jun-Hyung
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea ; SCM Lifescience Co., Ltd., 366 Saohae-daero, Jung-gu, Incheon 400-711, Korea.
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Shin
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea.
authors.forename: Seon Ji
authors.initials: SJ
authors.lastname: Tak
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea.
authors.forename: Kyuheon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746, Korea.
authors.forename: Youn Sook
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea.
authors.forename: Myung-Shin
authors.initials: MS
authors.lastname: Jeon
authors.affiliation: Digestive Disease Center, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam 463-838, Korea.
authors.forename: Ki-Baik
authors.initials: KB
authors.lastname: Hahm
authors.affiliation: Translational Research Center and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 100 Inharo, Nam-gu, Incheon 400-711, Korea ; SCM Lifescience Co., Ltd., 366 Saohae-daero, Jung-gu, Incheon 400-711, Korea.
authors.forename: Sun U
authors.initials: SU
authors.lastname: Song
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon 440-746, Korea.
authors.forename: Seok Hee
authors.initials: SH
authors.lastname: Park
keywords: anti-inflammation; clonal MSCs; colitis; inflammatory bowel disease; mucosal regeneration