title: Nondestructive evaluation of photosynthesis by delayed luminescence in Arabidopsis in Petri dishes.
abstract: Nondestructive evaluation of photosynthesis is a valuable tool in the field and laboratory. Delayed luminescence (DL) can reflect charge recombination through the backflow of electrons. However, DL detection has not yet been adapted for whole plants in Petri dishes. To compensate for differences in DL decay between sibling Arabidopsis plants grown under the same conditions, we developed a time-sequential double measurement method. Using this method, we examined the influence of photosynthetic electron flow inhibitors, and differences in the DL decay curves were categorized by considering the initial and late phases of the decay curves, as well as their intermediate slopes. The appearance of concavity and convexity in DL curves in Arabidopsis was different from unicellular algae, suggesting complexity in the photosynthetic machinery of higher plants. This detection method should be invaluable for evaluating photosynthetic defects in higher plants under sterile conditions without interrupting plant culture.
authors.affiliation: a Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Paweena
authors.initials: P
authors.lastname: Chuenwarin
authors.affiliation: a Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Azumi
authors.initials: A
authors.lastname: Shimazaki
authors.affiliation: b School of Health Promotional Science , Tokoha University , Hamamatsu , Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: c Central Research Laboratory , Hamamatsu Photonics K.K. , Hamamatsu , Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: c Central Research Laboratory , Hamamatsu Photonics K.K. , Hamamatsu , Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Katsumata
authors.affiliation: a Laboratory of Plant Molecular Improvement, Graduate School of Nutritional and Environmental Sciences , University of Shizuoka , Shizuoka , Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kobayashi
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000502:physiology*; D049449:Luminescence; D010788:Photosynthesis
keywords: 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB); 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU); Arabidopsis thaliana; antimycin A; delayed luminescence