title: Arctigenin from Fructus Arctii (Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers.
abstract: Fructus Arctii is used as a traditional herbal medicine to treat inflammatory diseases in oriental countries. This study aimed to investigate effect of F. Arctii extract on intestinal barrier function in human intestinal epithelial Caco-2 cells and to reveal the active component of F. Arctii. We measured transepithelial electrical resistance (TEER) value (as an index of barrier function) and ovalbumin (OVA) permeation (as an index of permeability) to observe the changes of intestinal barrier function. The treatment of F. Arctii increased TEER value and decreased OVA influx on Caco-2 cell monolayers. Furthermore, we found that arctigenin as an active component of F. Arctii increased TEER value and reduced permeability of OVA from apical to the basolateral side but not arctiin. In the present study, we revealed that F. Arctii could enhance intestinal barrier function, and its active component was an arctigenin on the functionality. We expect that the arctigenin from F. Arctii could contribute to prevention of inflammatory, allergic, and infectious diseases by reinforcing intestinal barrier function.
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Kyeonggi-do 463-746, Republic of Korea ; Division of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Kyeonggi-do 463-746, Republic of Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Kyeonggi-do 463-746, Republic of Korea.
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Back
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Kyeonggi-do 463-746, Republic of Korea.
authors.forename: Jeong-Ryong
authors.initials: JR
authors.lastname: Do
authors.affiliation: Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Kyeonggi-do 463-746, Republic of Korea ; Division of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea.
authors.forename: Dong-Hwa
authors.initials: DH
authors.lastname: Shon