title: γδ T cells support gut Ag-reactive colitogenic effector T-cell generation by enhancing Ag presentation by CD11b(+) DCs in the mesenteric LN.
abstract: T cells expressing the γδ TCR are dominant T-cell subsets in the intestinal immune system. We previously demonstrated that γδ T cells play important roles in augmenting Th17-type colitogenic immune responses in a T-cell-induced colitic inflammation model. However, its underlying mechanism remains poorly understood. In this study, an in vitro coculture system using effector T cells enriched in gut Ag-reactive cells was employed as a readout tool to search for gut Ag presenting APCs. We found that the presence of γδ T cells dramatically enhances gut Ag presentation within the mLN in mice. Gut Ag presentation by CD11b(+) DC subsets was particularly controlled by γδ T cells. Interestingly, γδ T-cell entry to the lymph nodes was essential to improve the Ag presentation. Therefore, our results highlight that γδ T cells play a previously unrecognized role to support colitogenic immunity by regulating gut Ag presentation in the draining LN.
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Jeongsu
authors.initials: J
authors.lastname: Do
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Anabelle
authors.initials: A
authors.lastname: Visperas
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Freeman
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Jang
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Sohee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Case 906, Institut National de la Santé et de la Recherche Médicale, U631, Centre National de la Recherche Scientifique, UMR6102, Marseille, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.affiliation: Department of Immunology, Lerner Research Institute, Lerner Research InstituteCleveland Clinic Foundation, Cleveland, OH, USA.
authors.forename: Booki
authors.initials: B
authors.lastname: Min
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D002478:Cells, Cultured; D003092:Colitis / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D006801:Humans; D018928:Immunity, Mucosal; D007422:Intestines / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008643:Mesentery / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: Colitis; DCs; Gut Ag; Presentation; γδ T cells