title: Structure and function of longin SNAREs.
abstract: Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins constitute the core membrane fusion machinery of intracellular transport and intercellular communication. A little more than ten years ago, it was proposed that the long N-terminal domain of a subset of SNAREs, henceforth called the longin domain, could be a crucial regulator with multiple functions in membrane trafficking. Structural, biochemical and cell biology studies have now produced a large set of data that support this hypothesis and indicate a role for the longin domain in regulating the sorting and activity of SNAREs. Here, we review the first decade of structure-function data on the three prototypical longin SNAREs: Ykt6, VAMP7 and Sec22b. We will, in particular, highlight the conserved molecular mechanisms that allow longin domains to fold back onto the fusion-inducing SNARE coiled-coil domain, thereby inhibiting membrane fusion, and describe the interactions of longin SNAREs with proteins that regulate their intracellular sorting. This dual function of the longin domain in regulating both the membrane localization and membrane fusion activity of SNAREs points to its role as a key regulatory module of intracellular trafficking.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut Jacques Monod, CNRS UMR 7592, Membrane Traffic in Health & Disease, INSERM ERL U950, Paris F-75013, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Daste
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut Jacques Monod, CNRS UMR 7592, Membrane Traffic in Health & Disease, INSERM ERL U950, Paris F-75013, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Galli
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, Institut Jacques Monod, CNRS UMR 7592, Membrane Traffic in Health & Disease, INSERM ERL U950, Paris F-75013, France david.tareste@inserm.fr.
authors.forename: David
authors.initials: D
authors.lastname: Tareste
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D006801:Humans; D017434:Protein Structure, Tertiary; D050600:SNARE Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D050600:SNARE Proteins
keywords: Lipid transfer; Longin; Membrane proteins; SNARE; Vesicular trafficking