title: Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels.
abstract: The quantification of circulating thrombin is a valuable tool to accurately assess the activity of the blood coagulation system. Here, we describe the combined application of the thrombin-specific reversible active-site inhibitor argatroban and the DNA-aptamer HD1-22 for conduction of an enzyme capture assay for reliable measurement of plasma thrombin levels.
authors.affiliation: Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Müller
authors.affiliation: Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Becher
authors.affiliation: Life & Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Mayer
authors.affiliation: Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Pötzsch
mesh_terms: D000991:Antithrombins / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D010875:Pipecolic Acids / Q000494:pharmacology; D013449:Sulfonamides; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000991:Antithrombins; D052157:Aptamers, Nucleotide; D010875:Pipecolic Acids; D013449:Sulfonamides; D001120:Arginine; D013917:Thrombin; C031942:argatroban
keywords: Active-site inhibitor; Aptamer; Argatroban; Enzyme capture assay; Plasma; Thrombin