title: Kallikrein Promotes Inflammation in Human Dental Pulp Cells Via Protease-Activated Receptor-1.
abstract: Plasma kallikrein (KLKB1), a serine protease, cleaves high-molecular weight kininogen to produce bradykinin, a potent vasodilator and pro-inflammatory peptide. In addition, KLKB1 activates plasminogen and other leukocyte and blood coagulation factors and processes pro-enkephalin, prorenin, and C3. KLKB1 has also been shown to cleave protease-activated receptors in vascular smooth muscle cells to regulate the expression of epidermal growth factor receptor. In this study, we investigated KLKB1-dependent inflammation and activation of protease-activated receptor-1 in human dental pulp cells. These cells responded to KLKB1 stimulation by increasing intracellular Ca(2+) , upregulating cyclooxygenase-2, and secreting prostaglandin E2 . Remarkably, SCH79797, an antagonist of protease-activated receptor-1, blocked these effects. Thus, these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1. J. Cell. Biochem. 117: 1522-1528, 2016. © 2015 Wiley Periodicals, Inc.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Hayama
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Kamio
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.
authors.forename: Tatsu
authors.initials: T
authors.lastname: Okabe
authors.affiliation: Department of Pulp Biology and Endodontics, Graduate School of Dentistry, Kanagawa Dental University, Yokosuka, Kanagawa, 238-8580, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Muromachi
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.|Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D002478:Cells, Cultured; D003176:Complement C3 / Q000378:metabolism; D003782:Dental Pulp / Q000378:metabolism* / Q000473:pathology; D004745:Enkephalins / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007610:Kallikreins / Q000378:metabolism*; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D011671:Pulpitis / Q000378:metabolism* / Q000473:pathology; D011758:Pyrroles / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology; D044463:Receptor, PAR-1 / Q000378:metabolism*; D012083:Renin / Q000378:metabolism
chemical_list: D003176:Complement C3; D004745:Enkephalins; C415424:N3-cyclopropyl-7-((4-(1-methylethyl)phenyl)methyl)-7H-pyrrolo(3, 2-f)quinazoline-1,3-diamine; D011498:Protein Precursors; D011758:Pyrroles; D011799:Quinazolines; D044463:Receptor, PAR-1; C029992:proenkephalin; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen; D012083:Renin
keywords: DENTAL PULP; INFLAMMATION; KALLIKREIN; PROTEASE-ACTIVATED RECEPTOR; PULPITIS