title: Development of a SIDA-LC-MS/MS Method for the Determination of Phomopsin A in Legumes.
abstract: A novel method for the determination of phomopsin A (1) in lupin flour, pea flour, and bean flour as well as whole lupin plants was established based on stable isotope dilution assay (SIDA) LC-MS/MS using (15)N6-1 as an isotopically labeled internal standard. Artificially infected samples were used to develop an optimized extraction procedure and sample pretreatment. The limits of detection were 0.5-1 μg/kg for all matrices. The limits of quantitation were 2-4 μg/kg. The method was used to analyze flour samples generated from selected legume seeds and lupin plant samples that had been inoculated with Diaporthe toxica and two further fungal strains. Finally, growing lupin plants infected with D. toxica were investigated to simulate a naturally in-field mycotoxicosis. Toxin levels of up to 10.1 μg/kg of 1 were found in the pods and 7.2 μg/kg in the stems and leaves.
authors.affiliation: BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Straße 11, 12489 Berlin, Germany.|University of Hamburg, Hamburg School of Food Science , Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Svenja
authors.initials: S
authors.lastname: Schloß
authors.affiliation: BAM Federal Institute for Materials Research and Testing , Richard-Willstätter-Straße 11, 12489 Berlin, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Koch
authors.affiliation: University of Hamburg, Hamburg School of Food Science , Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Rohn
authors.affiliation: University of Hamburg, Hamburg School of Food Science , Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.|Leibniz-Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V. , Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
authors.forename: Ronald
authors.initials: R
authors.lastname: Maul
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007553:Isotope Labeling / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D009842:Oligopeptides
chemical_list: D009183:Mycotoxins; C035753:phomopsin; D009842:Oligopeptides
keywords: Diaporthe toxica; isotopically labeled internal standard; legume contamination; stable isotope dilution assay