title: The Metabolomic Profile of Spent Culture Media from Day-3 Human Embryos Cultured under Low Oxygen Tension.
abstract: Despite efforts made to improve the in vitro embryo culture conditions used during assisted reproduction procedures, human embryos must adapt to different in vitro oxygen concentrations and the new metabolic milieu provided by the diverse culture media used for such protocols. It has been shown that the embryo culture environment can affect not only cellular metabolism, but also gene expression in different species of mammalian embryos. Therefore we wanted to compare the metabolic footprint left by human cleavage-stage embryos under two types of oxygen atmospheric culture conditions (6% and 20% O2). The spent culture media from 39 transferred and implanted embryos from a total of 22 patients undergoing egg donation treatment was analyzed; 23 embryos came from 13 patients in the 6% oxygen concentration group, and 16 embryos from 9 patients were used in the 20% oxygen concentration group. The multivariate statistics we used in our analysis showed that human cleavage-stage embryos grown under both types of oxygen concentration left a similar metabolic fingerprint. We failed to observe any change in the net depletion or release of relevant analytes, such as glucose and especially fatty acids, by human cleavage-stage embryos under either type of culture condition. Therefore it seems that low oxygen tension during embryo culture does not alter the global metabolism of human cleavage-stage embryos.
authors.affiliation: IVI Valencia, Valencia, Spain.|INCLIVA Biomedical Research and Fundación IVI, Valencia, Spain.
authors.forename: Maria José
authors.initials: MJ
authors.lastname: de Los Santos
authors.affiliation: IVI Valencia, Valencia, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Gámiz
authors.affiliation: IVI Valencia, Valencia, Spain.|INCLIVA Biomedical Research and Fundación IVI, Valencia, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: de Los Santos
authors.affiliation: IVI Valencia, Valencia, Spain.
authors.forename: Josep Lluís
authors.initials: JL
authors.lastname: Romero
authors.affiliation: IVI Sevilla, Sevilla, Spain.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Prados
authors.affiliation: OWL, Derio, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Alonso
authors.affiliation: IVI Valencia, Valencia, Spain.|INCLIVA Biomedical Research and Fundación IVI, Valencia, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Remohí
authors.affiliation: INCLIVA Biomedical Research and Fundación IVI, Valencia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Dominguez
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods; D017077:Culture Media, Conditioned / Q000378:metabolism*; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors
chemical_list: D017077:Culture Media, Conditioned; D010100:Oxygen