title: Comparative Proteomic Analysis of Two Varieties of Genetically Modified (GM) Embrapa 5.1 Common Bean (Phaseolus vulgaris L.) and Their Non-GM Counterparts.
abstract: The genetically modified (GM) common bean event Embrapa 5.1 was commercially approved in Brazil in 2011; it is resistant to golden mosaic virus infection. In the present work grain proteome profiles of two Embrapa 5.1 common bean varieties, Pérola and Pontal, and their non-GM counterparts were compared by two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry (MS). Analyses detected 23 spots differentially accumulated between GM Pérola and non-GM Pérola and 21 spots between GM Pontal and non-GM Pontal, although they were not the same proteins in Pérola and Pontal varieties, indicating that the variability observed may not be due to the genetic transformation. Among them, eight proteins were identified in Pérola varieties, and four proteins were identified in Pontal. Moreover, we applied principal component analysis (PCA) on 2-DE data, and variation between varieties was explained in the first two principal components. This work provides a first 2-DE-MS/MS-based analysis of Embrapa 5.1 common bean grains.
authors.affiliation: Food Science and Technology Department, Federal University of Santa Catarina , Rod. Admar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil.
authors.forename: Geisi M
authors.initials: GM
authors.lastname: Balsamo
authors.affiliation: Food Science and Technology Department, Federal University of Santa Catarina , Rod. Admar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil.
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Valentim-Neto
authors.affiliation: Food Science and Technology Department, Federal University of Santa Catarina , Rod. Admar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil.
authors.forename: Carla S
authors.initials: CS
authors.lastname: Mello
authors.affiliation: Food Science and Technology Department, Federal University of Santa Catarina , Rod. Admar Gonzaga 1346, 88034-001 Florianópolis, Santa Catarina, Brazil.
authors.forename: Ana C M
authors.initials: AC
authors.lastname: Arisi
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D030841:Food, Genetically Modified; D027805:Phaseolus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins
keywords: 2-DE; GMO; MS/MS; PCA; common bean; proteome