title: Proteomic Approaches for Biomarker Panels in Cancer.
abstract: Proteomic technologies remain the main backbone of biomarkers discovery in cancer. The continuous development of proteomic technologies also enlarges the bioinformatics domain, thus founding the main pillars of cancer therapy. The main source for diagnostic/prognostic/therapy monitoring biomarker panels are molecules that have a dual role, being both indicators of disease development and therapy targets. Proteomic technologies, such as mass-spectrometry approaches and protein array technologies, represent the main technologies that can depict these biomarkers. Herein, we will illustrate some of the most recent strategies for biomarker discovery in cancer, including the development of immune-markers and the use of cancer stem cells as target therapy. The challenges of proteomic biomarker discovery need new forms of cross-disciplinary conglomerates that will result in increased and tailored access to treatments for patients; diagnostic companies would benefit from the enhanced co-development of companion diagnostics and pharmaceutical companies. In the technology optimization in biomarkers, immune assays are the leaders of discovery machinery.
authors.affiliation: a Victor Babes National Institute of Pathology , Bucharest , Romania.|b Faculty of Medicine , Titu Maiorescu University , Bucharest , Romania.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Tanase
authors.affiliation: a Victor Babes National Institute of Pathology , Bucharest , Romania.|c National Institute for Chemical-Pharmaceutical R&D , Bucharest , Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Albulescu
authors.affiliation: a Victor Babes National Institute of Pathology , Bucharest , Romania.|d Faculty of Biology , Bucharest University , Bucharest , Romania.
authors.forename: Monica
authors.initials: M
authors.lastname: Neagu
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007118:Immunoassay; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D040081:Protein Array Analysis; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015398:Signal Transduction
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: cancer; immune-markers; mass spectrometry; protein arrays; protein biomarker panels; proteomics; signaling pathway; stem cells