title: Biological and immunotoxicity evaluation of antimicrobial peptide-loaded coatings using a layer-by-layer process on titanium.
abstract: The prevention and control of peri-implantitis is a challenge in dental implant surgery. Dental implants with sustained antimicrobial coating are an ideal way of preventing peri-implantitis. This study reports development of a non- immunotoxicity multilayered coating on a titanium surface that had sustained antimicrobial activity and limited early biofilm formation. In this study, the broad spectrum AMP, Tet213, was linked to collagen IV through sulfo-SMPB and has been renamed as AMPCol. The multilayer AMPCol coatings were assembled on smooth titanium surfaces using a LBL technique. Using XPS, AFM, contact angle analysis, and QCM, layer-by-layer accumulation of coating thickness was measured and increased surface wetting compared to controls was confirmed. Non-cytotoxicity to HaCaT and low erythrocyte hemolysis by the AMPCol coatings was observed. In vivo immunotoxicity assays showed IP administration of AMPCol did not effect serum immunoglobulin levels. This coating with controlled release of AMP decreased the growth of both a Gram-positive aerobe (Staphylococcus aureus) and a Gram-negative anaerobe (Porphyromonas gingivalis) up to one month. Early S. aureus biofilm formation was inhibited by the coating. The excellent long-term sustained antimicrobial activity of this multilayer coating is a potential method for preventing peri-implantitis through coated on the neck of implants before surgery.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oral Medicine, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Shifang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Implantology, Stomatology Hospital, School of Medical, Zhejiang university, Yan'an Road, Hangzhou 310006, China.
authors.forename: Guoli
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000276:immunology / Q000494:pharmacology / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000276:immunology / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology / Q000633:toxicity; D015921:Dental Implants; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D057873:Peri-Implantitis; D056951:Photoelectron Spectroscopy; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D013718:Tensile Strength; D014025:Titanium
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D014025:Titanium