title: Antimycobacterial action of a new glycolipid-peptide complex obtained from extracellular metabolites of Raoultella ornithinolytica.
abstract: In this paper, an antimycobacterial component of extracellular metabolites of a gut bacterium Raoultella ornithinolytica from D. veneta earthworms was isolated and its antimycobacterial action was tested using Mycobacterium smegmatis. After incubation with the complex obtained, formation of pores and furrows in cell walls was observed using microscopic techniques. The cells lost their shape, stuck together and formed clusters. Surface-enhanced Raman spectroscopy analysis showed that, after incubation, the complex was attached to the cell walls of the Mycobacterium. Analyses of the component performed with Fourier transform infrared spectroscopy demonstrated high similarity to a bacteriocin nisin, but energy dispersive X-ray spectroscopy analysis revealed differences in the elemental composition of this antimicrobial peptide. The component with antimycobacterial activity was identified using mass spectrometry techniques as a glycolipid-peptide complex. As it exhibits no cytotoxicity on normal human fibroblasts, the glycolipid-peptide complex appears to be a promising compound for investigations of its activity against pathogenic mycobacteria.
authors.affiliation: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Marta J
authors.initials: MJ
authors.lastname: Fiołka
authors.affiliation: Department of Biochemistry, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grzywnowicz
authors.affiliation: Analytical Laboratory, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Ewaryst
authors.initials: E
authors.lastname: Mendyk
authors.affiliation: Isobolographic Analysis Laboratory, Institute of Rural Health, Lublin, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Zagaja
authors.affiliation: Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, Łódź
authors.forename: Rafał
authors.initials: R
authors.lastname: Szewczyk
authors.affiliation: Analytical Laboratory, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Rawski
authors.affiliation: Analytical Laboratory, Faculty of Chemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Keller
authors.affiliation: Department of Biology and Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Rzymowska
authors.affiliation: Department of Comparative Anatomy and Anthropology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Wydrych
mesh_terms: D000818:Animals; D000904:Antibiotics, Antitubercular / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004755:Enterobacteriaceae / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D009561:Nisin / Q000737:chemistry / Q000494:pharmacology; D009835:Oligochaeta / Q000382:microbiology*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000904:Antibiotics, Antitubercular; D006017:Glycolipids; D010455:Peptides; D009561:Nisin; C102526:nisin A
keywords: Antimicrobial activity; Mycobacterium smegmatis; bacterial metabolites; natural product