title: The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis.
abstract: The guts of metazoans are in permanent contact with the microbial realm that includes beneficial symbionts, nonsymbionts, food-borne microbes and life-threatening pathogens. However, little is known concerning how host immunity affects gut bacterial community. Here, we analyze the role of a dual oxidase gene (BdDuox) in regulating the intestinal bacterial community homeostasis of the oriental fruit fly Bactrocera dorsalis. The results showed that knockdown of BdDuox led to an increased bacterial load, and to a decrease in the relative abundance of Enterobacteriaceae and Leuconostocaceae bacterial symbionts in the gut. The resulting dysbiosis, in turn, stimulates an immune response by activating BdDuox and promoting reactive oxygen species (ROS) production that regulates the composition and structure of the gut bacterial community to normal status by repressing the overgrowth of minor pathobionts. Our results suggest that BdDuox plays a pivotal role in regulating the homeostasis of the gut bacterial community in B. dorsalis.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Global Health Institute, Station 19, EPFL, Lausanne, Switzerland.
authors.forename: Bruno
authors.identifier: http://orcid.org/0000-0001-7970-1667
authors.initials: B
authors.lastname: Lemaitre
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Entomology, Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004755:Enterobacteriaceae / Q000145:classification* / Q000254:growth & development* / Q000378:metabolism; D000069196:Gastrointestinal Microbiome; D006706:Homeostasis; D056546:Lactobacillales / Q000145:classification* / Q000254:growth & development* / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D017382:Reactive Oxygen Species; D017422:Sequence Analysis, DNA; D033621:Tephritidae / Q000201:enzymology / Q000235:genetics / Q000382:microbiology*; D059467:Transcriptome
chemical_list: D017382:Reactive Oxygen Species; D010088:Oxidoreductases