title: Applications of Aptamers in Flow and Imaging Cytometry.
abstract: Aptamers compete with antibodies in many applications, in which high-affinity and specificity ligands are needed. In this regard, fluorescence-tagged aptamers have gained applications in flow and imaging cytometry for detecting cells expressing distinct antigens. Here we present prospective methods, as a starting point, for using these high-affinity ligands for cytometry applications.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, 05508-900, Brazil.
authors.forename: Isis C
authors.initials: IC
authors.lastname: Nascimento
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, 05508-900, Brazil.
authors.forename: Arthur A
authors.initials: AA
authors.lastname: Nery
authors.affiliation: Laboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Vinícius
authors.initials: V
authors.lastname: Bassaneze
authors.affiliation: Laboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: José E
authors.initials: JE
authors.lastname: Krieger
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo, 05508-900, Brazil. henning@iq.usp.br.
authors.forename: Henning
authors.initials: H
authors.lastname: Ulrich
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D019044:Image Cytometry / Q000379:methods*; D007700:Kinetics; D011485:Protein Binding; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D052157:Aptamers, Nucleotide
keywords: Aptamers; Automation; Flow cytometry; Fluorescence-labeling; Imaging cytometry