title: miR-539 induces cell cycle arrest in nasopharyngeal carcinoma by targeting cyclin-dependent kinase 4.
abstract: Dysregulation of microRNAs has been demonstrated to contribute to malignant progression of cancers, including nasopharyngeal carcinoma (NPC). miR-539 was previously reported to be significantly downregulated in osteosarcoma. However, the potential role and mechanism of action of miR-539 in the initiation and progression of NPC remain largely unknown. Quantitative reverse transcription (RT)-PCR demonstrated that miR-539 was significantly downregulated in NPC tumour tissues compared with nontumour tissues. The cell viability, colony formation assay and tumourigenicity assays in nude mice showed that miR-539 could inhibit NPC cell growth in vitro and in vivo. The cyclin-dependent kinase 4 (CDK4) was verified as a miR-539 target gene using dual-luciferase reporter assays, quantitative RT-PCR and Western blotting and was involved in miR-539-regulated NPC cell growth. These results indicated that miR-539 plays an important role in the initiation and progression of NPC by targeting CDK4 and the miR-539/CDK4 pathway may contribute to the development of novel therapeutic strategies for NPC in the future.
authors.affiliation: Department of Otolaryngology, Affiliated Liaocheng Second People Hospital, Taishan Medical College, Liaocheng, China.
authors.forename: Ling-yan
authors.initials: LY
authors.lastname: Lv
authors.affiliation: Department of Otolaryngology, Affiliated Liaocheng Second People Hospital, Taishan Medical College, Liaocheng, China.
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Affiliated Liaocheng Second People Hospital, Taishan Medical College, Liaocheng, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Beijing Tongren Hospital, Beijing, China.
authors.forename: Hong-rui
authors.initials: HR
authors.lastname: Zang
authors.affiliation: Department of Otolaryngology, Affiliated Liaocheng Second People Hospital, Taishan Medical College, Liaocheng, China.
authors.forename: Xing-jie
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002277:Carcinoma; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: C552081:MIRN539 microRNA, human; D035683:MicroRNAs; D051358:Cyclin-Dependent Kinase 4
keywords: CDK4; cell cycle; miR-539; nasopharyngeal carcinoma