title: Litomosoides sigmodontis: a jird urine metabolome study.
abstract: The neglected tropical disease onchocerciasis affects more than 35 million people worldwide with over 95% in Africa. Disease infection initiates from the filarial parasitic nematode Onchocerca volvulus, which is transmitted by the blackfly vector Simulium sp. carrying infectious L3 larvae. New treatments and diagnostics are required to eradicate this parasitic disease. Herein, we describe that a previously discovered biomarker for onchocerciasis, N-acetyltyramine-O-glucuronide (NATOG) is also present in urine samples of jirds infected with the onchocerciasis model nematode Litomosoides sigmodontis. Increased NATOG values paralleled a progressing infection and demonstrated that quantification of NATOG in this rodent model can be utilized to track its infectivity. Moreover, our findings suggest how NATOG monitoring may be used for evaluating potential drug candidates.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States; Department of Immunology, The Skaggs Institute for Chemical Biology, The Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Globisch
authors.affiliation: Institute of Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Sigmund Freud Straße 25, 53105 Bonn, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Specht
authors.affiliation: Institute of Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Sigmund Freud Straße 25, 53105 Bonn, Germany.
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Pfarr
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States; Department of Immunology, The Skaggs Institute for Chemical Biology, The Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Eubanks
authors.affiliation: Institute of Medical Microbiology, Immunology, and Parasitology (IMMIP), University Hospital Bonn, Sigmund Freud Straße 25, 53105 Bonn, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Hoerauf
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States; Department of Immunology, The Skaggs Institute for Chemical Biology, The Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, United States. Electronic address: kdjanda@scripps.edu.
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D005370:Filarioidea / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology; D005849:Gerbillinae; D020719:Glucuronides / Q000652:urine*; D008018:Life Cycle Stages; D055442:Metabolome; D009855:Onchocerciasis / Q000469:parasitology / Q000473:pathology* / Q000662:veterinary; D025341:Principal Component Analysis
chemical_list: D015415:Biomarkers; D020719:Glucuronides
keywords: Biomarker; Litomosoides sigmodontis; Metabolomics; Neurotransmitter; Onchocerciasis