title: Fermentation and genomic analysis of acetone-uncoupled butanol production by Clostridium tetanomorphum.
abstract: In typical acetone-butanol-ethanol (ABE) fermentation, acetone is the main by-product (50 % of butanol mass) of butanol production, resulting in a low yield of butanol. It is known that some Clostridium tetanomorphum strains are able to produce butanol without acetone in nature. Here, we described that C. tetanomorphum strain DSM665 can produce 4.16 g/L butanol and 4.98 g/L ethanol at pH 6.0, and 9.81 g/L butanol and 1.01 g/L ethanol when adding 1 mM methyl viologen. Butyrate and acetate could be reassimilated and no acetone was produced. Further analysis indicated that the activity of the acetate/butyrate:acetoacetyl-CoA transferase responsible for acetone production is lost in C. tetanomorphum DSM665. The genome of C. tetanomorphum DSM665 was sequenced and deposited in DDBJ, EMBL, and GenBank under the accession no. APJS00000000. Sequence analysis indicated that there are no typical genes (ctfA/B and adc) that are typically parts of an acetone synthesis pathway in C. tetanomorphum DSM665. This work provides new insights in the mechanism of clostridial butanol production and should prove useful for the design of a high-butanol-producing strain.
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Fuyu
authors.initials: F
authors.lastname: Gong
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Guanhui
authors.initials: G
authors.lastname: Bao
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China.|University of the Chinese Academy of Sciences, Beijing, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China. zhangyp@im.ac.cn.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing, China. redarmy305@126.com.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Dong
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048012:Clostridium tetanomorphum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D016680:Genome, Bacterial; D023281:Genomics; D008969:Molecular Sequence Data
chemical_list: D001426:Bacterial Proteins; D000096:Acetone; D020001:1-Butanol
keywords: Acetone; Butanol; Clostridium tetanomorphum; Fermentation; adc; ctfA/B