title: Increased binding of circulating systemic lupus erythematosus autoantibodies to recombinant interferon alpha 2b.
abstract: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by various types of immunological abnormalities including circulating and tissue-fixed autoantibodies reactive with autoantigens. The mechanism that can explain the production of these antibodies is unclear. Here we address the binding specificity of SLE autoantibodies with recombinant alpha interferon 2b (hrIFN α-2b), commercially available interferon (IFN α-2b), and the gene (cIFN α-2b) encoding this interferon. hrIFN α-2b showed higher binding with naturally occurring SLE autoantibodies as compared to IFN α-2b (p < 0.05) or cIFN α-2b gene (p < 0.001) as assessed by direct binding, inhibition ELISA, and quantitative precipitin titration. The relative affinity of SLE autoantibodies for hrIFN α-2b, IFN α-2b, and cIFN α-2b gene was in the order of 1.13 × 10(-7) , 1.38 × 10(-6) , and 1.22 × 10(-6) , respectively. hrIFN α-2b is shown to have unique epitopes that would explain the possible antigenic role of hrIFN α-2b in the generation of SLE autoantibodies. Anti-hrIFN α-2b antibodies have been shown to represent an alternative immunological probe for the estimation of interferon alpha 2b in the serum of SLE patients.
authors.affiliation: Department of Clinical Biochemistry, College of Medicine, King Khalid University, Abha, Saudi Arabia.
authors.forename: Wahid Ali
authors.initials: WA
authors.lastname: Khan
authors.affiliation: Department of Clinical Biochemistry, College of Medicine, King Khalid University, Abha, Saudi Arabia.
authors.forename: Javed Anwer
authors.initials: JA
authors.lastname: Qureshi
mesh_terms: D000915:Antibody Affinity / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001379:Azathioprine / Q000627:therapeutic use; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use; D008875:Middle Aged; D011239:Prednisolone / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000276:immunology*
chemical_list: D001323:Autoantibodies; C554158:IFNA2 protein, human; D016898:Interferon-alpha; D011994:Recombinant Proteins; D011239:Prednisolone; D001379:Azathioprine; D008727:Methotrexate
keywords: ELISA; Recombinant interferon alpha 2b (hrINF α-2b); affinity; antibodies; systemic lupus erythematosus