title: Isolation of a novel polyomavirus, related to Japanese eel endothelial cell-infecting virus, from marbled eels, Anguilla marmorata (Quoy & Gaimard).
abstract: Marbled eels, Anguilla marmorata (Quoy & Gaimard), cultured in Taiwan exhibited haemorrhage and mortality in January 2012. The severely diseased eels bled from the gills and showed congestion of the central venous sinus of the gill filaments and haemorrhage throughout the body similar to viral endothelial cell necrosis of eel. In this study, a novel polyomavirus (AmPyV) was isolated from the diseased eels using the AMPF cell line established from the pectoral fin of healthy marbled eels. AmPyV was found to encode a long T-antigen orthologous gene. Phylogenetic analysis showed that AmPyV was closely related to Japanese eel endothelial cell-infecting virus. PCR assays revealed AmPyV infection throughout the systemic organs. AmPyV proliferated in the AMPF, EK-1 and EO-2 cells at temperatures 25-30 °C, and the progeny virus yields were 10(7.0) , 10(7.4) and 10(7.7) TCID50  mL(-1) , respectively. The purified virions were icosahedral particles, 70-80 nm in diameter. No clinical signs or mortality was observed among the eels injected with the virus; however, the virus was reisolated from the brain, eyes, kidneys, fins and gills of infected eels 2 month after injection. Our results suggest that AmPyV exhibits a latent infection. Pathogen of the disease needs to study further.
authors.affiliation: Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.
authors.forename: C M
authors.initials: CM
authors.lastname: Wen
authors.affiliation: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
authors.forename: M M
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan.
authors.forename: C S
authors.initials: CS
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: P C
authors.initials: PC
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: F H
authors.initials: FH
authors.lastname: Nan
mesh_terms: D000595:Amino Acid Sequence; D000811:Anguilla; D000818:Animals; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011120:Polyomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D027601:Polyomavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment / Q000662:veterinary; D013624:Taiwan; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Anguilla; T-antigen; eel diseases; haemorrhage; polyomavirus; viral endothelial cell necrosis of eel