title: Multiple abiotic stress tolerance of the transformants yeast cells and the transgenic Arabidopsis plants expressing a novel durum wheat catalase.
abstract: Catalases are reactive oxygen species scavenging enzymes involved in response to abiotic and biotic stresses. In this study, we described the isolation and functional characterization of a novel catalase from durum wheat, designed TdCAT1. Molecular Phylogeny analyses showed that wheat TdCAT1 exhibited high amino acids sequence identity to other plant catalases. Sequence homology analysis showed that TdCAT1 protein contained the putative calmodulin binding domain and a putative conserved internal peroxisomal targeting signal PTS1 motif around its C-terminus. Predicted three-dimensional structural model revealed the presence of four putative distinct structural regions which are the N-terminal arm, the β-barrel, the wrapping and the α-helical domains. TdCAT1 protein had the heme pocket that was composed by five essential residues. TdCAT1 gene expression analysis showed that this gene was induced by various abiotic stresses in durum wheat. The expression of TdCAT1 in yeast cells and Arabidopsis plants conferred tolerance to several abiotic stresses. Compared with the non-transformed plants, the transgenic lines maintained their growth and accumulated more proline under stress treatments. Furthermore, the amount of H2O2 was lower in transgenic lines, which was due to the high CAT and POD activities. Taken together, these data provide the evidence for the involvement of durum wheat catalase TdCAT1 in tolerance to multiple abiotic stresses in crop plants.
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Kaouthar
authors.initials: K
authors.lastname: Feki
authors.affiliation: Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Kamoun
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Rihem
authors.initials: R
authors.lastname: Ben Mahmoud
authors.affiliation: Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Ameny
authors.initials: A
authors.lastname: Farhat-Khemakhem
authors.affiliation: Laboratory of Molecular Biotechnologie of Eukaryotes, Centre of Biotechnology of Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
authors.affiliation: Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, Tunisia. Electronic address: faical.brini@cbs.rnrt.tn.
authors.forename: Faiçal
authors.initials: F
authors.lastname: Brini
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D000975:Antioxidants / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D002374:Catalase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological / Q000235:genetics; D014170:Transformation, Genetic; D014908:Triticum / Q000201:enzymology*
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D012333:RNA, Messenger; D011392:Proline; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: Abiotic stress; Antioxidant enzymes; Arabidopsis; Catalase; Oxidative stress; Triticum durum