title: A chicken homologue of nectin-4 functions as a measles virus receptor.
abstract: Measles virus (MV) vaccine strains use CD46, signaling lymphocyte activation molecule, and nectin-4 in human cells as receptors. Meanwhile, many of them are propagated in primary chicken embryonic fibroblasts (CEFs). Our data revealed that CEFs express a nectin-4 homologous molecule (CEF nectin-4) containing well-conserved motifs in the FG and BC loops, but not in the C'C″ loop. MV infected CHO cells expressing CEF nectin-4 and induced syncytia in these cells, confirming that CEF nectin-4 functions as an MV receptor and that the C'C″ loop is not critical for this function. Nectin-4-blind mutations in MV H protein reduced the infectivity of MV in CEF nectin-4-expressing cells. Infection of CEFs with the MV vaccine AIK-C strain was partially blocked by an anti-nectin-4 antibody, indicating that CEF nectin-4 plays a role for propagation of MV vaccines in CEFs.
authors.affiliation: Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: fseki@nih.go.jp.
authors.forename: Fumio
authors.initials: F
authors.lastname: Seki
authors.affiliation: Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Someya
authors.affiliation: Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Komase
authors.affiliation: Department of Virology 3, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeda
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D005347:Fibroblasts / Q000821:virology*; D008459:Measles virus / Q000502:physiology*; D000075983:Nectins; D011991:Receptors, Virus / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: D015815:Cell Adhesion Molecules; D000075983:Nectins; D011991:Receptors, Virus
keywords: Chicken embryonic fibroblasts; Measles virus; Nectin-4; Viral receptor