title: Temporal variation of Bistorta vivipara-associated ectomycorrhizal fungal communities in the High Arctic.
abstract: Ectomycorrhizal (ECM) fungi are important for efficient nutrient uptake of several widespread arctic plant species. Knowledge of temporal variation of ECM fungi, and the relationship of these patterns to environmental variables, is essential to understand energy and nutrient cycling in Arctic ecosystems. We sampled roots of Bistorta vivipara ten times over two years; three times during the growing-season (June, July and September) and twice during winter (November and April) of both years. We found 668 ECM OTUs belonging to 25 different ECM lineages, whereof 157 OTUs persisted throughout all sampling time-points. Overall, ECM fungal richness peaked in winter and species belonging to Cortinarius, Serendipita and Sebacina were more frequent in winter than during summer. Structure of ECM fungal communities was primarily affected by spatial factors. However, after accounting for spatial effects, significant seasonal variation was evident revealing correspondence with seasonal changes in environmental conditions. We demonstrate that arctic ECM richness and community structure differ between summer (growing-season) and winter, possibly due to reduced activity of the core community, and addition of fungi adapted for winter conditions forming a winter-active fungal community. Significant month × year interactions were observed both for fungal richness and community composition, indicating unpredictable between-year variation. Our study indicates that addressing seasonal changes requires replication over several years.
authors.affiliation: The University Centre in Svalbard, P.O. Box 156, Longyearbyen, NO-9171, Norway.|Section for Genetics and Evolutionary Biology, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, Oslo, NO-0316, Norway.
authors.forename: Sunil
authors.initials: S
authors.lastname: Mundra
authors.affiliation: Institute of Ecology and Earth Sciences, Tartu University, 14A Ravila, Tartu, 50411, Estonia.|Department of Organismal Biology, Evolutionary Biology Centre, Uppsala University, Uppsala, SE 75236, Sweden.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bahram
authors.affiliation: Natural History Museum, University of Tartu, 14A Ravila, Tartu, 50411, Estonia.
authors.forename: Leho
authors.initials: L
authors.lastname: Tedersoo
authors.affiliation: Section for Genetics and Evolutionary Biology, Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, Oslo, NO-0316, Norway.
authors.forename: Håvard
authors.initials: H
authors.lastname: Kauserud
authors.affiliation: Natural History Museum, University of Oslo, Oslo, Norway.
authors.forename: Rune
authors.initials: R
authors.lastname: Halvorsen
authors.affiliation: The University Centre in Svalbard, P.O. Box 156, Longyearbyen, NO-9171, Norway.
authors.forename: Pernille Bronken
authors.initials: PB
authors.lastname: Eidesen
mesh_terms: D001110:Arctic Regions; D044822:Biodiversity; D004271:DNA, Fungal; D059014:High-Throughput Nucleotide Sequencing; D038821:Mycorrhizae / Q000145:classification* / Q000235:genetics; D018517:Plant Roots / Q000382:microbiology; D019664:Polygonaceae / Q000382:microbiology*; D012621:Seasons; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004271:DNA, Fungal
keywords: Arctic; Bistorta vivipara; Illumina sequencing; Svalbard; ectomycorrhizal fungi; fungal richness and community; metagenomics/community genomics; seasonal/temporal variation