title: New species of Torque Teno miniviruses infecting gorillas and chimpanzees.
abstract: Anelloviridae family is comprised of small, non-enveloped viruses of various genome lengths, high sequence diversity, sharing the same genome organization. Infections and co-infections by different genotypes in humans are ubiquitous. Related viruses were described in number of mammalian hosts, but very limited data are available from the closest human relatives - great apes and non-human primates. Here we report the 100% prevalence determined by semi-nested PCR from fecal samples of 16 captive primate species. Only the Mandrillus sphinx, showed the prevalence only 8%. We describe three new species of gorillas׳ and four new species of chimpanzees׳ Betatorqueviruses and their co-infections in one individual. This study is also first report and analysis of nearly full length TTMV genomes infecting gorillas. Our attempts to sequence the complete genomes of anelloviruses from host feces invariably failed. Broader usage of blood /tissue material is necessary to understand the diversity and interspecies transmission of anelloviruses.
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic. Electronic address: kristyna@hrazdilova.cz.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hrazdilová
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Slaninková
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Brožová
authors.affiliation: CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; Department of Pathology and Parasitology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Branišovská 31, 37005 České Budějovice, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Modrý
authors.affiliation: The Prague Zoological Garden, Prague 171 00, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Vodička
authors.affiliation: Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic; CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno,612 42 Brno, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Celer
mesh_terms: D000818:Animals; D018420:Ape Diseases / Q000453:epidemiology* / Q000821:virology; D001483:Base Sequence; D060085:Coinfection / Q000235:genetics; D004266:DNA Virus Infections / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral / Q000235:genetics*; D006071:Gorilla gorilla / Q000821:virology*; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000821:virology*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000145:classification / Q000235:genetics*
chemical_list: D004279:DNA, Viral
keywords: Anellovirus; Genome sequence; Great apes; Non-human primates; Torque Teno mini virus