title: Association Between Graft Storage Time and Donor Age With Endothelial Cell Density and Graft Adherence After Descemet Membrane Endothelial Keratoplasty.
abstract: OBJECTIVE
  After retrospectively evaluating the clinical outcome of 500 consecutive cases after Descemet membrane endothelial keratoplasty (DMEK), we extended the analysis in this study by assessing the effect of donor-related parameters on endothelial cell density (ECD) decline and detachment rate in this group.
  
  
  METHODS
  This retrospective case series included 500 cases who had undergone DMEK from October 2007 to September 2012 at the Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands. Logistic regression analysis (n = 332 eyes) showed that donor age might be associated with a 3% increase in the risk for a detachment (odds ratio, 0.97; 95% CI, 0.94-1.00; P = .049) (ie, higher donor age seems to be associated with lower chances of a detachment). In addition, linear regression analysis indicated that graft storage time in medium was associated with ECD decrease (ie, the longer the storage time, the larger the decrease at 6 months after DMEK) (P = .01).
  
  
  CONCLUSIONS
  We showed an association between graft storage time and ECD decline after DMEK and possibly between donor age and graft detachment. Therefore, donor storage times should be kept as short as possible to improve short-term ECDs. More research is needed to draw definite conclusions on the possible effect of donor age on the chance of a detachment after DMEK.
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands2Melles Cornea Clinic, Rotterdam, the Netherlands.
authors.forename: Marina
authors.initials: M
authors.lastname: Rodríguez-Calvo de Mora
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands3Amnitrans EyeBank, Rotterdam, the Netherlands.
authors.forename: Esther A
authors.initials: EA
authors.lastname: Groeneveld-van Beek
authors.affiliation: Department of Methodology and Statistics, Utrecht University, Utrecht, the Netherlands.
authors.forename: Laurence E
authors.initials: LE
authors.lastname: Frank
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands3Amnitrans EyeBank, Rotterdam, the Netherlands.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: van der Wees
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands2Melles Cornea Clinic, Rotterdam, the Netherlands.
authors.forename: Silke
authors.initials: S
authors.lastname: Oellerich
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands2Melles Cornea Clinic, Rotterdam, the Netherlands.
authors.forename: Marieke
authors.initials: M
authors.lastname: Bruinsma
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery (NIIOS), Rotterdam, the Netherlands2Melles Cornea Clinic, Rotterdam, the Netherlands3Amnitrans EyeBank, Rotterdam, the Netherlands.
authors.forename: Gerrit R J
authors.initials: GR
authors.lastname: Melles
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D055954:Corneal Endothelial Cell Loss / Q000209:etiology* / Q000503:physiopathology; D057111:Descemet Stripping Endothelial Keratoplasty; D004728:Endothelium, Corneal; D005260:Female; D006084:Graft Rejection / Q000209:etiology* / Q000503:physiopathology; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation; D012189:Retrospective Studies; D013997:Time Factors; D014019:Tissue Donors