title: Potential of the Lectin/Inhibitor Isolated from Crataeva tapia Bark (CrataBL) for Controlling Callosobruchus maculatus Larva Development.
abstract: Callosobruchus maculatus is an important predator of cowpeas. Due to infestation during storage, this insect affects the quality of seed and crop yield. This study aimed to investigate the effects of CrataBL, a multifunction protein isolated from Crataeva tapia bark, on C. maculatus larva development. The protein, which is stable even in extreme pH conditions, showed toxic activity, reducing the larval mass 45 and 70% at concentrations of 0.25 and 1.0% (w/w), respectively. Acting as an inhibitor, CrataBL decreased by 39% the activity of cysteine proteinases from larval gut. Conversely, the activity of serine proteinases was increased about 8-fold. The toxic properties of CrataBL may also be attributed to its capacity of binding to glycoproteins or glycosaminoglycans. Such binding interferes with larval metabolism, because CrataBL-FITC was found in the fat body, Malpighian tubules, and feces of larvae. These results demonstrate the potential of this protein for controlling larva development.
authors.affiliation: Departamento de Bioquı́mica, Universidade Federal de São Paulo-UNIFESP-EPM , 04044-020 São Paulo, SP, Brazil.
authors.forename: Natalia N S
authors.initials: NN
authors.lastname: Nunes
authors.affiliation: Departamento de Bioquı́mica, Universidade Federal de São Paulo-UNIFESP-EPM , 04044-020 São Paulo, SP, Brazil.
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Ferreira
authors.affiliation: Centro de Engenharia e Ciências Exatas, Universidade Estadual do Oeste do Paraná , Toledo, Paraná, Brazil.
authors.forename: Rosemeire A
authors.initials: RA
authors.lastname: Silva-Lucca
authors.affiliation: Laboratório de Quı́mica e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia-CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro-UENF , Campos dos Goytacazes, RJ, Brazil.
authors.forename: Leonardo F R
authors.initials: LF
authors.lastname: de Sá
authors.affiliation: Laboratório de Quı́mica e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia-CBB, Universidade Estadual do Norte Fluminense Darcy Ribeiro-UENF , Campos dos Goytacazes, RJ, Brazil.
authors.forename: Antônia Elenir A
authors.initials: AE
authors.lastname: de Oliveira
authors.affiliation: Departamento de Bioquı́mica, Universidade Federal de Pernambuco , Recife, PE, Brazil.
authors.forename: Maria Tereza dos S
authors.initials: MT
authors.lastname: Correia
authors.affiliation: Departamento de Bioquı́mica, Universidade Federal de Pernambuco , Recife, PE, Brazil.
authors.forename: Patrícia Maria G
authors.initials: PM
authors.lastname: Paiva
authors.affiliation: Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702, United States.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wlodawer
authors.affiliation: Departamento de Bioquı́mica, Universidade Federal de São Paulo-UNIFESP-EPM , 04044-020 São Paulo, SP, Brazil.
authors.forename: Maria Luiza V
authors.initials: ML
authors.lastname: Oliva
mesh_terms: D000818:Animals; D031483:Capparaceae / Q000737:chemistry*; D001517:Coleoptera / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D057056:Cysteine Proteases / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D019476:Insect Proteins / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D037102:Lectins / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D019476:Insect Proteins; D037102:Lectins; D010936:Plant Extracts; D057056:Cysteine Proteases
keywords: C. maculatus; Crataeva tapia; bioinsecticide; glycosaminoglycan; inhibitor; lectin