title: Biogenesis of autophagosomal precursors for LC3 lipidation from the ER-Golgi intermediate compartment.
abstract: Autophagosome biogenesis requires efficient mobilization and delivery of membranes from intracellular sources. How these membranes are mobilized remains poorly understood. Our recent work reported an autophagic signal-induced membrane mobilization event from the ER-Golgi intermediate compartment (ERGIC) to generate an early autophagosomal membrane precursor. We found that starvation activates the autophagic phosphatidylinositol 3-kinase, which promotes a relocation of COPII proteins from the ER-exit sites to the ERGIC. The relocation of COPII generates ERGIC-derived COPII vesicles as a membrane template for LC3 lipidation, a key step for autophagosome biogenesis.
authors.affiliation: a Department of Molecular and Cell Biology and Howard Hughes Medical Institute ; University of California ; Berkeley , CA USA.
authors.forename: Liang
authors.initials: L
authors.lastname: Ge
authors.affiliation: a Department of Molecular and Cell Biology and Howard Hughes Medical Institute ; University of California ; Berkeley , CA USA.
authors.forename: Livia
authors.initials: L
authors.lastname: Wilz
authors.affiliation: a Department of Molecular and Cell Biology and Howard Hughes Medical Institute ; University of California ; Berkeley , CA USA.
authors.forename: Randy
authors.initials: R
authors.lastname: Schekman
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D019869:Phosphatidylinositol 3-Kinases
keywords: COPII; ER-Golgi intermediate compartment; LC3; autophagosome; autophagy; phosphatidylinositol 3-kinase