title: A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat.
abstract: As there are numerous pathogen species that cause disease and limit yields of crops, such as wheat (Triticum aestivum), single genes that provide resistance to multiple pathogens are valuable in crop improvement. The mechanistic basis of multi-pathogen resistance is largely unknown. Here we use comparative genomics, mutagenesis and transformation to isolate the wheat Lr67 gene, which confers partial resistance to all three wheat rust pathogen species and powdery mildew. The Lr67 resistance gene encodes a predicted hexose transporter (LR67res) that differs from the susceptible form of the same protein (LR67sus) by two amino acids that are conserved in orthologous hexose transporters. Sugar uptake assays show that LR67sus, and related proteins encoded by homeoalleles, function as high-affinity glucose transporters. LR67res exerts a dominant-negative effect through heterodimerization with these functional transporters to reduce glucose uptake. Alterations in hexose transport in infected leaves may explain its ability to reduce the growth of multiple biotrophic pathogen species.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: John W
authors.initials: JW
authors.lastname: Moore
authors.affiliation: International Maize and Wheat Improvement Center (CIMMYT), México DF, Mexico.
authors.forename: Sybil
authors.initials: S
authors.lastname: Herrera-Foessel
authors.affiliation: International Maize and Wheat Improvement Center (CIMMYT), México DF, Mexico.
authors.forename: Caixia
authors.initials: C
authors.lastname: Lan
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Wendelin
authors.initials: W
authors.lastname: Schnippenkoetter
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Michael
authors.initials: M
authors.lastname: Ayliffe
authors.affiliation: Campo Experimental Valle de México INIFAP, Chapingo, Edo de México, México.
authors.forename: Julio
authors.initials: J
authors.lastname: Huerta-Espino
authors.affiliation: Department of Plant Sciences, Norwegian University of Life Sciences, Ås, Norway.
authors.forename: Morten
authors.initials: M
authors.lastname: Lillemo
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Libby
authors.initials: L
authors.lastname: Viccars
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Ricky
authors.initials: R
authors.lastname: Milne
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Sambasivam
authors.initials: S
authors.lastname: Periyannan
authors.affiliation: The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Kong
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Spielmeyer
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Talbot
authors.affiliation: The University of Sydney Plant Breeding Institute-Cobbitty, Narellan, Australia.
authors.forename: Harbans
authors.initials: H
authors.lastname: Bariana
authors.affiliation: The University of Newcastle, School of Environmental &Life Sciences, Callaghan, Australia.
authors.forename: John W
authors.initials: JW
authors.lastname: Patrick
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Dodds
authors.affiliation: International Maize and Wheat Improvement Center (CIMMYT), México DF, Mexico.
authors.forename: Ravi
authors.initials: R
authors.lastname: Singh
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture, Canberra, Australia.
authors.forename: Evans
authors.initials: E
authors.lastname: Lagudah
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology*
chemical_list: D009004:Monosaccharide Transport Proteins; D010940:Plant Proteins