title: Use of a porcine urinary bladder acellular matrix for corneal reconstruction in dogs and cats.
abstract: OBJECTIVE
  To describe the use of a porcine urinary bladder acellular matrix for surgical reconstruction of the cornea in cases of canine and feline deep corneal ulcers, and feline corneal sequestra.
  
  
  METHODS
  Twenty-seven dogs and three cats with deep corneal ulcers and seven cats with corneal sequestra were included in the study with overall 38 eyes. For each patient, the necrotic material (ie corneal sequestrum or collagenolytic tissue) was removed by circular lamellar keratectomy. The collagen graft was then cut and prepared to match the stromal defect and then sutured into the lamellar keratectomy bed using interrupted and continuous patterns of absorbable polyglactin 9-0 sutures. Postoperative medical treatment consisted of topical and systemic administration of antibiotics, combined with topical administration of atropine sulfate. The animals were examined 18, 45, and 90 days after the surgery.
  
  
  RESULTS
  Postoperative examination revealed complete integration of the biomaterial in 93.5% of ulceration cases in both species and in 100% of feline corneal sequestrum cases. In two cases of ulceration (1 dog and 1 cat), progression of the collagenolytic process at the graft periphery required an additional conjunctival graft 7 days after the first surgery. At 90 days post-op, 100% of the eyes were sighted.
  
  
  CONCLUSIONS
  Use of a porcine urinary bladder acellular matrix appears to be effective in the surgical management of deep corneal ulcers and feline corneal sequestra.
authors.affiliation: Centre Hospitalier Vétérinaire, 95 rue des Mazurots, Ludres, 54710, France. balland@lorrainevet.fr.
authors.forename: Olivier
authors.initials: O
authors.lastname: Balland
authors.affiliation: Centre Hospitalier Vétérinaire, 95 rue des Mazurots, Ludres, 54710, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Poinsard
authors.affiliation: Clinique vétérinaire des Acacias, 42 Avenue Lucien-Servanty, Blagnac, 31700, France.
authors.forename: Frank
authors.identifier: http://orcid.org/0000-0001-6199-9063
authors.initials: F
authors.lastname: Famose
authors.affiliation: Clinique Vétérinaire, Parc d'activité Mermoz, 19 Avenue de la forêt, Eysines, 33320, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Goulle
authors.affiliation: Centre Hospitalier Vétérinaire, 275 route Impériale, Saint-Martin Bellevue, 74370, France.
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Isard
authors.affiliation: Eyevet Referrals, 41-43 Halton Station Road, Sutton Weaver, Cheshire, WA7 3DN, United Kingdom.
authors.forename: Iona
authors.initials: I
authors.lastname: Mathieson
authors.affiliation: Centre Hospitalier Vétérinaire, 275 route Impériale, Saint-Martin Bellevue, 74370, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dulaurent
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000601:surgery*; D002415:Cats; D003315:Cornea / Q000601:surgery*; D003316:Corneal Diseases / Q000601:surgery; D003320:Corneal Ulcer / Q000601:surgery / Q000662:veterinary*; D004283:Dog Diseases / Q000601:surgery*; D004285:Dogs; D013552:Swine; D001743:Urinary Bladder
keywords: acell; acellular matrix; cat; dog; sequestrum; ulcer