title: Isolation and analysis of discreet human prostate cellular populations.
abstract: The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.
authors.affiliation: Department of Urology, UT Southwestern University Medical Center, Dallas, TX, USA.
authors.forename: Douglas W
authors.initials: DW
authors.lastname: Strand
authors.affiliation: Department of Cancer Biology, Meharry Medical College, Nashville, TN, USA.
authors.forename: LaTayia
authors.initials: L
authors.lastname: Aaron
authors.affiliation: Department of Urology, UT Southwestern University Medical Center, Dallas, TX, USA.
authors.forename: Gervaise
authors.initials: G
authors.lastname: Henry
authors.affiliation: Department of Surgery, NorthShore University Health System, Research Institute, Evanston, IL, USA.
authors.forename: Omar E
authors.initials: OE
authors.lastname: Franco
authors.affiliation: Department of Surgery, NorthShore University Health System, Research Institute, Evanston, IL, USA. Electronic address: shayward@northshore.org.
authors.forename: Simon W
authors.initials: SW
authors.lastname: Hayward
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002469:Cell Separation / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D011467:Prostate / Q000166:cytology* / Q000254:growth & development