title: Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins.
abstract: A convenient reverse micellar one-pot reaction yields multifunctional silica nanoparticles, which can be tailored to effectively suppress non-specific adsorption and, at the same time, enable efficient specific covalent immobilization of proteins. Using two highly sensitive proteins, it is demonstrated that the new particles provide a suitable microenvironment to maintain the protein's activity.
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Wang
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Kersten S
authors.initials: KS
authors.lastname: Rabe
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.affiliation: Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG-1), Hermann-von-Helmholtz-Platz, 76344, Eggenstein-Leopoldshafen, Germany.
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques; D005947:Glucose / Q000032:analysis; D055767:Immobilized Proteins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D055767:Immobilized Proteins; D013438:Sulfhydryl Compounds; D012822:Silicon Dioxide; D005947:Glucose
keywords: biomolecules; immobilization; proteins; silica nanoparticles; surface chemistry