title: The association of HPV genotype with the regression, persistence or progression of low-grade squamous intraepithelial lesions.
abstract: BACKGROUND
  Human papillomavirus (HPV) is a highly prevalent sexually transmitted virus causing cytological alterations that precede cervical cancer. Approximately 130 genotypes have been sequenced. Low-grade squamous intraepithelial lesions (LSIL) are the most frequent cytological alteration and have an uncertain behavior.
  
  
  OBJECTIVE
  To analyze the frequency of HPV types in LSIL and their association with the regression, persistence or progression of these lesions.
  
  
  METHODS
  A cohort study of forty patients with LSIL cytology was conducted from December 2007 to March 2011. The follow-up lasted two years and included cytology and colposcopy. HPV detection was performed using PCR, and genotyping was performed using PCR-specific and RFLP techniques.
  
  
  RESULTS
  DNA-HPV was detected in 87% (35/40) of the cases, with oncogenic HPV accounting for 76%; type 16 in 32% (11/35) and type 18 in 20%. LSIL regression, persistence and progression rates at the end of the study were 60%, 23% and 17%, respectively. There was 50% regression in lesions in the high oncogenic risk group (types 16 and 18).
  
  
  CONCLUSIONS
  HPV 16 was the most frequent genotype found in LSIL. The persistence and progression of the LSIL were related to the persistence of oncogenic HPV. The longer the follow-up time, the lower the LSIL persistence rate and the higher its regression rate; the progression rate remained stable. In addition to the presence of oncogenic HPV, other factors are necessary for the progression of LSIL.
authors.affiliation: Institute of Gynecology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil; Post-Graduate Program in Surgical Sciences, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: F A
authors.initials: FA
authors.lastname: Silveira
authors.affiliation: Institute of Gynecology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil; Post-Graduate Program in Surgical Sciences, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: G
authors.initials: G
authors.lastname: Almeida
authors.affiliation: Institute of Gynecology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil; Post-Graduate Program in Surgical Sciences, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil. Electronic address: yarafurtadorj@terra.com.br.
authors.forename: Y L
authors.initials: YL
authors.lastname: Furtado
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal Fluminense (UFF), Niterói, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Cavalcanti
authors.affiliation: National Institute for Women's, Children's and Adolescents' Health, Instituto Fernandes Figueira, Fundação Osvaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
authors.forename: K S
authors.initials: KS
authors.lastname: Silva
authors.affiliation: Institute of Gynecology, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Maldonado
authors.affiliation: Pathology Department, Laboratory of Molecular Pathology, Clementino Fraga Filho Hospital, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: M G C
authors.initials: MG
authors.lastname: Carvalho
mesh_terms: D018450:Disease Progression; D005260:Female; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications / Q000821:virology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000473:pathology* / Q000821:virology*
keywords: Cytology; Diagnosis; Genotyping techniques; PCR; Papillomavirus infections