title: Transcriptome profiling of the cysticercus stage of the laboratory model Taenia crassiceps, strain ORF.
abstract: Neurocysticercosis (NC) is a serious public health problem mainly in developing countries. NC caused by the cysticercus stage from cestode Taenia solium is considered by the WHO and ITFDE as a potentially eradicable disease. Definitive diagnosis of NC is challenging because of the unspecific clinical manifestations such as the non-definitive evidence presented by neuroimaging (in most cases) and the lack of definitive serological test. Taenia crassiceps (ORF strain) is a cestode closely related to T. solium and it has frequently been used as a source of antigens for immunodiagnostics. A murine model to study host immune response to infection has also been established by using T. crassiceps. Despite the extensive use of T. crassiceps for research, molecular information for this cestode is scarce in public databases. With the aim of providing more extensive information on T. crassiceps biology, an RNA-seq experiment and subsequent bioinformatic transcriptome processing of this cestode parasite mRNA in its cysticercus stage were carried out. A total of 227,082 read/ESTs were sequenced using the 454-GS FLX Titanium technology and assembled into 10,787 contigs. This transcriptome dataset represents new and valuable molecular information of the cestode T. crassiceps (ORF). This information will substantially improve public information and will help to achieve a better understanding of the biology of T. crassiceps and to identify target proteins for serodiagnosis and vaccination.
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.
authors.forename: Gisela M
authors.initials: GM
authors.lastname: García-Montoya
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Universidad de Antioquia, Colombia.
authors.forename: Jairo A
authors.initials: JA
authors.lastname: Mesa-Arango
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Universidad de Antioquia, Colombia.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Isaza-Agudelo
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia.
authors.forename: Sonia P
authors.initials: SP
authors.lastname: Agudelo-Lopez
authors.affiliation: Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Universidad de Antioquia, Colombia; Grupo Sistemas Embebidos e Inteligencia Computacional-SISTEMIC, Departamento de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Antioquia, Colombia.
authors.forename: Felipe
authors.initials: F
authors.lastname: Cabarcas
authors.affiliation: Grupo de Inmunología Celular e Inmunogenética, Facultad de Medicina, Universidad de Antioquia-GICIG, Colombia.
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barrera
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia, Colombia; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Universidad de Antioquia, Colombia. Electronic address: jfernando.alzate@udea.edu.co.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Alzate
mesh_terms: D000818:Animals; D003552:Cysticercus / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000175:diagnosis / Q000276:immunology*; D017434:Protein Structure, Tertiary; D012698:Serologic Tests; D013621:Taenia / Q000235:genetics*
keywords: 454 Pyrosequencing; Cysticercosis; Next generation sequencing-NGS; ORF strain; RNA-seq; Taenia crassiceps; Transcriptome