title: The voltage-gated proton channel Hv1 is expressed in pancreatic islet β-cells and regulates insulin secretion.
abstract: The voltage-gated proton channel Hv1 is a potent acid extruder that participates in the extrusion of the intracellular acid. Here, we showed for the first time, Hv1 is highly expressed in mouse and human pancreatic islet β-cells, as well as β-cell lines. Imaging studies demonstrated that Hv1 resides in insulin-containing granules in β-cells. Knockdown of Hv1 with RNA interference significantly reduces glucose- and K(+)-induced insulin secretion in isolated islets and INS-1 (832/13) β-cells and has an impairment on glucose- and K(+)-induced intracellular Ca(2+) homeostasis. Our data demonstrated that the expression of Hv1 in pancreatic islet β-cells regulates insulin secretion through regulating Ca(2+) homeostasis.
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: School of Medicine, Nankai University, Tianjin 300071, PR China.
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Che
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Shangrong
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Artificial Cell, Third Central Clinical College of Tianjin Medical University, Tianjin 300170, PR China.
authors.forename: Ying-Tang
authors.initials: YT
authors.lastname: Gao
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xi
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China.
authors.forename: Weiyan
authors.initials: W
authors.lastname: Zuo
authors.affiliation: Department of Biophysics, School of Physics Science, The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, PR China. Electronic address: shujieli@nankai.edu.cn.
authors.forename: Shu Jie
authors.initials: SJ
authors.lastname: Li
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000502:physiology*; D015640:Ion Channel Gating / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D051379:Mice
chemical_list: C510235:HVCN1 protein, human; D007328:Insulin; D007473:Ion Channels; D005947:Glucose; D002118:Calcium
keywords: Hv1; Insulin secretion; Pancreatic islets; Voltage-gated proton channel; β-cells