title: microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2.
abstract: miR-186 has been demonstrated to have a significant role as a tumor suppressor in many types of cancers. Nevertheless, its biological function in esophageal squamous cell carcinoma (ESCC) remains unknown. In the present study, we found that the expression level of miR-186 was downregulated in ESCC in comparison with the adjacent normal tissues and was significantly associated with differentiation level, TNM stage, and lymph node metastasis of ESCC. Functional experiments revealed that enforced overexpression of miR-186 in ESCC cells suppressed the proliferation, invasion, and induced the apoptosis of cells. Luciferase reporter assay and western blotting analysis were performed to verify the target gene regulated by miR-186, SKP2. Our findings established that the miR-186 has a suppressive role in ESCC progression via SKP2-mediated pathway, and this implies that miR-186 could be a potential therapeutic target for ESCC.
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Experimental Teaching Center of Medical Functional Science, Medical College of Henan University of Science and Technology, Luoyang, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Wenqiao
authors.initials: W
authors.lastname: Zang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D000077277:Esophageal Squamous Cell Carcinoma; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000032:analysis; D044786:S-Phase Kinase-Associated Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: C533096:MIRN186 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D044786:S-Phase Kinase-Associated Proteins