title: Effects of Double Transgenesis of Somatotrophic Axis (GH/GHR) on Skeletal Muscle Growth of Zebrafish (Danio rerio).
abstract: Transgenic fish for growth hormone (GH) has been considered as a potential technological improvement in aquaculture. In this study, a double-transgenic zebrafish was used to evaluate the effect of GH and its receptor (GHR) on muscle growth. Double transgenics reached the same length of GH transgenic, but with significantly less weight, featuring an unbalanced growth. The condition factor of GH/GHR-transgenic fish was lower than the other genotypes. Histological analysis showed a decrease in the percentage of thick muscle fibers in GH/GHR genotype of ∼ 80% in comparison to GH-transgenic line. The analysis of gene expression showed a significant decrease in genes related to muscle growth in GH/GHR genotype. It seems that concomitant overexpression of GH and GHR resulted in a strong decrease of the somatotrophic axis intracellular signaling by diminishing its principal transcription factor signal transducer and activator of transcription 5.1 (STAT5.1).
authors.affiliation: 1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Ana Cecilia Gomes
authors.initials: AC
authors.lastname: Silva
authors.affiliation: 1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Daniela Volcan
authors.initials: DV
authors.lastname: Almeida
authors.affiliation: 1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Bruna Felix
authors.initials: BF
authors.lastname: Nornberg
authors.affiliation: 1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Marcio Azevedo
authors.initials: MA
authors.lastname: Figueiredo
authors.affiliation: 2 Laboratório de Imunologia e Patologia de Organismos Aquáticos, Estação Marinha de Aquicultura, Instituto de Oceanografia, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Romano
authors.affiliation: 1 Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande-FURG , Rio Grande-RS, Brazil .
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Marins
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005838:Genotype; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000254:growth & development*; D011986:Receptors, Somatotropin / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D011986:Receptors, Somatotropin; D013006:Growth Hormone