title: Cu(2+)-mediated fluorescence switching of gold nanoclusters for the selective detection of clioquinol.
abstract: It is of great significance to sense clioquinol (CQ) in a simple and fast way because of its potential application in the treatment of neurodegenerative diseases. In this contribution, we proposed a Cu(2+)-mediated fluorescence switchable strategy to detect CQ by taking bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) as probes. It was found that the strong red fluorescence of BSA-protected AuNCs at 610 nm could be effectively quenched by Cu(2+) (off state) and reversibly recovered by CQ (on state) owing to the specific coordination of CQ and Cu(2+). Under the optimal conditions, there was a good linear relationship between the off-on efficiency (Eoff-on) and the amount of CQ in the range of 1-12 μM (R(2) = 0.9902), with a detection limit of 0.63 μM (3σ). The "turn off-on" mode and the fast and unique complexation of CQ and Cu(2+) endow AuNCs with high specificity for CQ sensing. The proposed strategy is label-free, fast and selective, which is applicable to the analysis of CQ in cream with satisfactory results.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Science, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chang
authors.affiliation: College of Physical Science and Technology, Southwest University, Chongqing 400715, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Shao Qing
authors.initials: SQ
authors.lastname: Lie
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Science, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn.
authors.forename: Peng Fei
authors.initials: PF
authors.lastname: Gao
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Science, Southwest University, Chongqing 400715, China. chengzhi@swu.edu.cn and Chongqing Key Laboratory of Biomedical Analysis (Southwest University), Chongqing Science & Technology Commission, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
mesh_terms: D000818:Animals; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D007464:Clioquinol / Q000032:analysis*; D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013997:Time Factors
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D003300:Copper; D007464:Clioquinol