title: Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging.
abstract: Acidic phospholipids are minor membrane lipids but critically important for signaling events. The main acidic phospholipids are phosphatidylinositol phosphates (PIPs also known as phosphoinositides), phosphatidylserine (PS), and phosphatidic acid (PA). Acidic phospholipids are precursors of second messengers of key signaling cascades or are second messengers themselves. They regulate the localization and activation of many proteins, and are involved in virtually all membrane trafficking events. As such, it is crucial to understand the subcellular localization and dynamics of each of these lipids within the cell. Over the years, several techniques have emerged in either fixed or live cells to analyze the subcellular localization and dynamics of acidic phospholipids. In this chapter, we review one of them: the use of genetically encoded biosensors that are based on the expression of specific lipid binding domains (LBDs) fused to fluorescent proteins. We discuss how to design such sensors, including the criteria for selecting the lipid binding domains of interest and to validate them. We also emphasize the care that must be taken during data analysis as well as the main limitations and advantages of this approach.
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 46 Allée d'Italie, 69364, Lyon Cedex 07, France.
authors.forename: Matthieu Pierre
authors.initials: MP
authors.lastname: Platre
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 46 Allée d'Italie, 69364, Lyon Cedex 07, France. yvon.jaillais@ens-lyon.fr.
authors.forename: Yvon
authors.initials: Y
authors.lastname: Jaillais
mesh_terms: D001692:Biological Transport; D015374:Biosensing Techniques; D057054:Molecular Imaging / Q000379:methods*; D015335:Molecular Probes; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs
chemical_list: D015335:Molecular Probes; D010743:Phospholipids
keywords: Biosensor; Genetically encoded probe s; Lipid binding domain; Lipid signaling; Live imaging; Phosphatidic acid; Phosphatidylinositol phosphate; Phosphatidylserine; Phospholipase; PtdIns