title: Standardisation of a novel sperm banking kit - NextGen(®) - to preserve sperm parameters during shipment.
abstract: Many male patients diagnosed with cancer are within their reproductive years. These men are advised to freeze their spermatozoa prior to the start of cancer treatment. Very often, sperm banking facilities may not be readily available and patients may be required to travel to distant sperm bank centres. Our objective was to design and standardise a remote home shipping sperm kit that allows patients to collect a semen sample at home and ship it overnight to a sperm bank. A total of 21 semen samples and two transport media (refrigeration media and human tubal fluid) and five different combinations of ice packs were tested for maintaining desired shipping temperature. Ten semen samples were assessed for pre- and post-shipment changes in sperm motility, membrane integrity, total motile spermatozoa and recovery of motile spermatozoa. Even though motility, membrane integrity and total motile spermatozoa declined both in samples examined under simulated shipped conditions and in overnight-shipped samples, the observed motility and total motile spermatozoa were adequate for use with assisted reproductive techniques. Using refrigeration media, cooling sleeve and ice packs, adequate sperm motility can be maintained utilising NextGen(®) kit and these spermatozoa can be used for procreation utilising ART techniques such as intracytoplasmic sperm injection.
authors.affiliation: Andrology Center and Reproductive Tissue Bank, and American Center for Reproductive Medicine, Cleveland, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: Andrology Center and Reproductive Tissue Bank, and American Center for Reproductive Medicine, Cleveland, OH, USA.|Health Services Department, Saint Joseph University, Philadelphia, PA, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Andrology Center and Reproductive Tissue Bank, and American Center for Reproductive Medicine, Cleveland, OH, USA.|The Ohio State University College of Medicine, Columbus, OH, USA.
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.affiliation: Andrology Center and Reproductive Tissue Bank, and American Center for Reproductive Medicine, Cleveland, OH, USA.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Andrology Center and Reproductive Tissue Bank, and American Center for Reproductive Medicine, Cleveland, OH, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sharma
mesh_terms: D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D013074:Sperm Banks; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
keywords: Home banking; human spermatozoa; hypoosmotic swelling; motility; sperm vitality