title: IgM-enriched solution BT086 improves host defense capacity and energy store preservation in a rabbit model of endotoxemia.
abstract: BACKGROUND
  The therapeutic value of intravenous immunoglobulin (IVIG) as an adjuvant therapy in sepsis remains debatable. We hypothesized that intravenous administration of BT086, a predominantly IgM IVIG solution, would improve host defense in an established rabbit model of endotoxemia and systemic sepsis.
  
  
  METHODS
  New Zealand white rabbits were randomized into the following four groups: (1) the negative control group without lipopolysaccharide (LPS, control), (2) the positive control group with LPS infusion (LPS group), (3) the albumin-treated LPS group (ALB+LPS group), and (4) the BT086-treated LPS group (BT086 + LPS group). A standardized amount of E. coli was intravenously injected into all of the animals. The vital parameters, the concentration of E. coli in the blood and other organs, the residual granulocyte phagocytosis activity, and the levels of the inflammatory mediators were measured. Histological changes in the lung and liver tissue were examined following autopsy.
  
  
  RESULTS
  The elimination of E. coli from the bloodstream was expedited in the BT086-treated group compared with the LPS- and albumin-treated groups. The BT086 + LPS group exhibited higher phagocytic activity of polymorphonuclear neutrophils (PMNs) than the control and ALB+LPS groups. The liver energy stores were higher in the BT086 + LPS group than in the other groups.
  
  
  CONCLUSIONS
  Our data suggest that the IgM-enriched IVIG has the potential to improve host defense in a rabbit model of endotoxemia. Studies using different animal models and dosages are necessary to further explore the potential benefits of IgM-enriched IVIG solutions.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Shmygalev
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Damm
authors.affiliation: Institute of Anatomy, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Knels
authors.affiliation: Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Strassburg
authors.affiliation: Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wünsche
authors.affiliation: Institute of Medical Microbiology and Hygiene, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Dumke
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Medical char Schleswig-Holstein, Lübeck, Germany.
authors.forename: S N
authors.initials: SN
authors.lastname: Stehr
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Koch
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany.
authors.forename: A R
authors.identifier: http://orcid.org/0000-0001-6144-3874
authors.initials: AR
authors.lastname: Heller
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology; D006439:Hemodynamics; D007075:Immunoglobulin M / Q000627:therapeutic use*; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use*; D009504:Neutrophils / Q000378:metabolism; D010587:Phagocytosis; D019999:Pharmaceutical Solutions / Q000627:therapeutic use; D011817:Rabbits; D016897:Respiratory Burst
chemical_list: D007075:Immunoglobulin M; D016756:Immunoglobulins, Intravenous; D019999:Pharmaceutical Solutions