title: Naringenin ameliorates renal and platelet purinergic signalling alterations in high-cholesterol fed rats through the suppression of ROS and NF-κB signaling pathways.
abstract: Naringenin (NGEN) is a natural flavonoid aglycone of naringin that has been reported to have a wide range of pharmacological properties, such as antioxidant activity and free radical scavenging capacity. The aim of this study was to investigate the protective effect of NGEN on oxidative and inflammatory parameters, as well as to evaluate the hydrolysis of adenine nucleotides in kidney and platelet membranes of rats exposed to a hypercholesterolemic diet (HCD) for 90 days. Kidney oxidative stress and mRNA expression of the ectonucleoside triphosphate diphosphohydrolases (NTPDases), ecto-5'-nucleotidase (CD73), inducible NO synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and the nuclear factor kappa B (NF-κB) genes were evaluated by real time RT-PCR. The co-administration of NGEN (50 mg kg(-1)) for 90 days significantly prevented renal failure in HCD rats as indicated by an improvement of renal markers. Histopathological observation findings are also consistent with these effects. Moreover, NGEN (50 mg kg(-1)) significantly decreased the lipid profile and inhibited pro-oxidant and inflammation marker levels in the kidney of HCD rats. Furthermore, the NTPDase activities were significantly decreased in platelets and kidney membranes of HCD-treated rats and these alterations were improved by NGEN. In conclusion, this study suggests that naringenin can potentially improve the renal failure and platelet alterations observed in rats fed a hypercholesterolemic diet probably through its antioxidant effects.
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia. yacine_chtourou@yahoo.fr.
authors.forename: Yassine
authors.initials: Y
authors.lastname: Chtourou
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia. yacine_chtourou@yahoo.fr.
authors.forename: Zeineb
authors.initials: Z
authors.lastname: Kamoun
authors.affiliation: Institute of Olive Tree, BP 1087, 3018 Sfax, Tunisia.
authors.forename: Wissem
authors.initials: W
authors.lastname: Zarrouk
authors.affiliation: Molecular Biology Laboratory, Faculty of Nature and Life Sciences, University of Jijel, PB 98, Ouled Aissa, 1800 Jijel, Algeria.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Kebieche
authors.affiliation: Hematology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia.
authors.forename: Choumous
authors.initials: C
authors.lastname: Kallel
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia. yacine_chtourou@yahoo.fr.
authors.forename: Radhouane
authors.initials: R
authors.lastname: Gdoura
authors.affiliation: Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of Sfax, Tunisia. yacine_chtourou@yahoo.fr.
authors.forename: Hamadi
authors.initials: H
authors.lastname: Fetoui
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D000975:Antioxidants; D001081:Apyrase / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D044950:Flavanones / Q000494:pharmacology*; D016166:Free Radical Scavengers; D015870:Gene Expression / Q000187:drug effects; D006868:Hydrolysis; D006937:Hypercholesterolemia / Q000150:complications / Q000378:metabolism; D007249:Inflammation / Q000235:genetics; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D016328:NF-kappa B / Q000378:metabolism*; D009708:Nucleotidases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D051437:Renal Insufficiency / Q000517:prevention & control; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000227:Adenine Nucleotides; D000975:Antioxidants; D002791:Cholesterol, Dietary; D044950:Flavanones; D016166:Free Radical Scavengers; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D009708:Nucleotidases; D001081:Apyrase; C005273:naringenin