title: Proteomic Analysis of Lonicera japonica Thunb. Immature Flower Buds Using Combinatorial Peptide Ligand Libraries and Polyethylene Glycol Fractionation.
abstract: Lonicera japonica Thunb. flower is a well-known medicinal plant that has been widely used for the treatment of human disease. To explore the molecular mechanisms underlying the biological activities of L. japonica immature flower buds, a gel-free/label-free proteomic technique was used in combination with combinatorial peptide ligand libraries (CPLL) and polyethylene glycol (PEG) fractionation for the enrichment of low-abundance proteins and removal of high-abundance proteins, respectively. A total of 177, 614, and 529 proteins were identified in crude protein extraction, CPLL fractions, and PEG fractions, respectively. Among the identified proteins, 283 and 239 proteins were specifically identified by the CPLL and PEG methods, respectively. In particular, proteins related to the oxidative pentose phosphate pathway, signaling, hormone metabolism, and transport were highly enriched by CPLL and PEG fractionation compared to crude protein extraction. A total of 28 secondary metabolism-related proteins and 25 metabolites were identified in L. japonica immature flower buds. To determine the specificity of the identified proteins and metabolites for L. japonica immature flower buds, Cerasus flower buds were used, which resulted in the abundance of hydroxymethylbutenyl 4-diphosphate synthase in L. japonica immature flower buds being 10-fold higher than that in Cerasus flower buds. These results suggest that proteins related to secondary metabolism might be responsible for the biological activities of L. japonica immature flower buds.
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.|College of Biomedical Engineering and Instrument Science, Zhejiang University , Hangzhou 310027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University , Hangzhou 310027, China.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University , Hangzhou 310027, China.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University , Hangzhou 310027, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D011232:Chemical Precipitation; D035264:Flowers / Q000378:metabolism*; D008024:Ligands; D031239:Lonicera / Q000378:metabolism*; D027845:Malus / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D019151:Peptide Library; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D020543:Proteome / Q000302:isolation & purification / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D008024:Ligands; D019151:Peptide Library; D010940:Plant Proteins; D020543:Proteome; D011092:Polyethylene Glycols
keywords: Lonicera japonica Thunb.; combinatorial peptide ligand libraries; immature flower buds; polyethylene glycol; proteomics