title: A new fluorescent dye for cell tracing and mitochondrial imaging in vitro and in vivo.
abstract: Mitochondria contribute to redox and calcium balance, and apoptosis thus regulating cellular fate. In the present study, mitochondrial staining applying a novel dye, V07-07059, was performed in human embryonic kidney cells, a human vascular endothelial cell line and primary human mononuclear cells. The new fluorescent mega Stokes dye (peak excitation: 488 nm, peak emission: 554 nm) showed superior fluorescent properties and stability. V07-07059 stains mitochondria dependent on their membrane potential and is safe to use in vitro and in vivo. Unlike other dyes applied in this context (e.g. Tetramethylrhodamine methyl ester), V07-07059 only marginally inhibits mitochondrial respiration and function. V07-07059 enables real time imaging of mitochondrial trafficking and remodeling. Prolonged staining with V07-07059 demonstrated the dyes suitability as a novel probe to track cells. In comparison to the widely used standard for cell proliferation and tracking studies 5(6)-diacetate N-succinimidyl ester, V07-07059 proved superior regarding toxicity and photostability.
authors.affiliation: Center for Sepsis Control & Care (CSCC), Jena University Hospital, Erlanger Allee 101, 07747, Jena, Germany.|Department of Anesthesiology & Critical Care, Jena University Hospital, Erlanger Allee 101, 07747, Jena.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.
authors.forename: Adrian T
authors.initials: AT
authors.lastname: Press
authors.affiliation: Center for Sepsis Control & Care (CSCC), Jena University Hospital, Erlanger Allee 101, 07747, Jena, Germany.|Department of Anesthesiology & Critical Care, Jena University Hospital, Erlanger Allee 101, 07747, Jena.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ungelenk
authors.affiliation: Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.|Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743, Jena, Germany.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Rinkenauer
authors.affiliation: Institute of Biochemistry II, Jena University Hospital, Friedrich Schiller University, 07743, Jena, Germany.
authors.forename: Marko
authors.initials: M
authors.lastname: Gröger
authors.affiliation: Dyomics GmbH, Jena Germany, Otto-Schott-Str. 15, 07745, Jena, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Lehmann
authors.affiliation: Center for Sepsis Control & Care (CSCC), Jena University Hospital, Erlanger Allee 101, 07747, Jena, Germany.|Institute of Biochemistry II, Jena University Hospital, Friedrich Schiller University, 07743, Jena, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Mosig
authors.affiliation: Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany.|Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743, Jena, Germany.
authors.forename: Ulrich S
authors.initials: US
authors.lastname: Schubert
authors.affiliation: Center for Sepsis Control & Care (CSCC), Jena University Hospital, Erlanger Allee 101, 07747, Jena, Germany.|Department of Biological Sciences and Center for Biomedical Engineering and Science, University of North Carolina at Charlotte, Charlotte, North Carolina, 28223, USA.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Clemens
authors.affiliation: Center for Sepsis Control & Care (CSCC), Jena University Hospital, Erlanger Allee 101, 07747, Jena, Germany. Michael.Bauer@med.uni-jena.de.|Department of Anesthesiology & Critical Care, Jena University Hospital, Erlanger Allee 101, 07747, Jena. Michael.Bauer@med.uni-jena.de.|Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743, Jena, Germany. Michael.Bauer@med.uni-jena.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Bauer
mesh_terms: D000818:Animals; D017209:Apoptosis; D042783:Endothelial Cells / Q000166:cytology; D005456:Fluorescent Dyes; D057809:HEK293 Cells; D006801:Humans; D000069416:Intravital Microscopy; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008297:Male; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes
keywords: cell tracing; dye; fluorescence; live cell imaging; mitochondria; staining