title: A single amino acid substitution in movement protein of tomato torrado virus influences ToTV infectivity in Solanum lycopersicum.
abstract: Tomato torrado virus (ToTV), which is a tomato-infecting member of the genus Torradovirus, induces severe systemic necrosis in Solanum lycopersicum cv. Beta Lux as well as leaf malformation and chlorosis in Nicotiana benthamiana. To date, neither the tomato gene conferring resistance to the pathogen nor the ToTV-encoded necrosis determinant have been characterized. We herein revealed that the phenylalanine 210 residue in the movement protein domain encoded by ToTV RNA2 is a necrosis-inducing pathogenicity determinant during tomato infection. Using a ToTV infectious RNA2 clone, we performed site-directed mutagenesis of the phenylalanine 210 residue, confirming its importance during ToTV infection and symptom manifestation in S. lycopersicum cv. Beta Lux, but not in N. benthamiana.
authors.affiliation: Interdepartmental Laboratory of Molecular Biology-National Research Institute, 20 WładysławaWęgorka St, Poznań 60-318, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Wieczorek
authors.affiliation: Interdepartmental Laboratory of Molecular Biology-National Research Institute, 20 WładysławaWęgorka St, Poznań 60-318, Poland. Electronic address: olaob@o2.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Obrępalska-Stęplowska
mesh_terms: D019943:Amino Acid Substitution; D018551:Solanum lycopersicum / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D010649:Phenylalanine / Q000235:genetics; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics / Q000472:pathogenicity*; D012328:RNA Viruses / Q000235:genetics / Q000472:pathogenicity*; D014026:Nicotiana / Q000821:virology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D053830:Plant Viral Movement Proteins; D037521:Virulence Factors; D010649:Phenylalanine
keywords: Movement protein; Mutation; Pathogenicity determinant; Plant response; Symptom expression; Tomato torrado virus