title: Long term effect of alkali types on waste activated sludge hydrolytic acidification and microbial community at low temperature.
abstract: The effect of four alkali reagents (NaOH, KOH, Ca(OH)2, mixed alkali) on waste activated sludge (WAS) hydrolytic acidification and microbial community was studied in semi-continuous fermentation systems at low temperature (15°C) over long term operational time (65day). The results showed that protein and polysaccharide of NaOH (124.26, 11.92) was similar to that of KOH (109.53, 11.30), both were higher than Ca(OH)2 (70.66, 3.74) and mixed alkali (90.66, 8.71). The short chain fatty acids (SCFAs) of NaOH (231.62) was higher than KOH (220.62mg chemical oxygen demand (COD)/g VSS). Although Ca(OH)2 system had strong acidification capacity, the shortage of SCFAs occurred due to the low activity of hydrolase. Illumina MiSeq sequencing revealed that Tissierella and Erysipelothrix were enriched in the NaOH and Ca(OH)2 systems, where Peptostreptococcaceae incertae_sedis was enriched in the NaOH and KOH systems, less Anaerolinea was involved in Ca(OH)2 condition.
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Baodan
authors.initials: B
authors.lastname: Jin
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address: wsy@bjut.edu.cn.
authors.forename: Shuying
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Xing
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China; Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Baikun
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000143:Acids; D000468:Alkalies / Q000737:chemistry*; D001421:Bacteria, Anaerobic / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002126:Calcium Hydroxide / Q000737:chemistry; D002412:Cations; D003080:Cold Temperature; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry; D006868:Hydrolysis; D059013:Microbial Consortia; D016133:Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology*; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D000143:Acids; D000468:Alkalies; D002412:Cations; D005232:Fatty Acids, Volatile; D012722:Sewage; D012972:Sodium Hydroxide; D006867:Hydrolases; D002126:Calcium Hydroxide
keywords: Alkali type; Hydrolytic acidification; Low temperature fermentation; Microbial community; Waste activated sludge