title: Insights into the proteomic response of soybean towards Al₂O₃, ZnO, and Ag nanoparticles stress.
abstract: Understanding the complex mechanisms involved in plant response to nanoparticles is indispensable in assessing the impact of nano-pollutants on environment. The present study compares the phytotoxicity of three different metal-based nanoparticles (Al2O3, ZnO, and Ag) in soybean seedling at proteome level. Plant growth, rigidity of roots, and root cell viability were markedly affected by ZnO- and Ag-NPs stress; while, Al2O3-NPs challenged soybean maintained normal seedling growth like control. Moreover, severe oxidative burst was evident in ZnO-NPs and Ag-NPs treatments. Gel-free proteomic analysis of NPs stressed soybean roots revealed 104 commonly changed proteins primarily associated with secondary metabolism, cell organization, and hormone metabolism. Oxidation-reduction cascade related genes, such as GDSL motif lipase 5, SKU5 similar 4, galactose oxidase, and quinone reductase were up-regulated in Al2O3-NPs challenged roots and down-regulated in ZnO- and Ag-NPs treatments. In comparison to root, 16 common proteins were found to be significantly changed in leaves of NPs exposed soybean that were predominantly associated to photosystem and protein degradation. The proteomic findings suggest that high abundance of proteins involved in oxidation-reduction, stress signaling, hormonal pathways related to growth and development might be the principal key for optimum growth of soybean under Al2O3-NPs stress.
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Department of Botany, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address: zahed_kly@yahoo.com.
authors.forename: Zahed
authors.initials: Z
authors.lastname: Hossain
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
authors.forename: Ghazala
authors.initials: G
authors.lastname: Mustafa
authors.affiliation: Maebashi Institute of Technology, Maebashi 371-0816, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Sakata
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan. Electronic address: skomatsu@affrc.go.jp.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D053758:Nanoparticles; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000494:pharmacology*; D013025:Glycine max / Q000187:drug effects* / Q000378:metabolism; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012834:Silver; D000537:Aluminum Oxide; D015034:Zinc Oxide
keywords: Al(2)O(3)-NP; Nanoparticles; Oxidative stress; Proteomics; Soybean