title: Upregulation of TGF-β1 and basic FGF in elastofibroma: an immunohistochemical analysis.
abstract: Elastofibroma is a rare tumour that occurs in the subscapular space, and it typically presents in middle-aged and older individuals. The aetiology of elastofibroma remains unknown. Recent, sporadic reports have shown, immunohistologically, that fibroblasts in elastofibroma may produce abnormal elastic and collagen fibres through the action of transforming growth factor-beta (TGF-β), a factor that promotes fibroblast proliferation. However, that finding lacked quantitative measurements and controls. Therefore, in this study, we performed quantitative, immunohistochemical analyses of TGF-β1 and basic fibroblast growth factor (bFGF) in three elastofibromas, and we compared them to ten dermatofibromas and keloids, and five normal skin. In elastofibroma specimens, 16-59 % fibroblasts were positive for TGF-β1 in the cytoplasm, compared to 96 % in dermatofibroma, 93 % in keloid and 2 % in normal dermis specimens. Also, in elastofibroma specimens, 26-67 % of fibroblasts were positive for bFGF in the cytoplasm, compared to 97 % in dermatofibroma, 97 % in keloid, and 22 % in normal dermis specimens. Intriguingly, the tumour size and growth rate were proportional to the percentage of cells positive for bFGF. Finally, greater levels of bFGF expressions in fibroblasts were associated with larger sized elastofibromas. These results suggested that elastofibroma development depended on high expression of TGF-β1 and bFGF.
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Imanishi
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan. imanishi12@med.osaka-cu.ac.jp.|Division of Dermatology, Saiseikai Tondabayashi Hospital, Osaka, Japan. imanishi12@med.osaka-cu.ac.jp.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Imanishi
authors.affiliation: Division of Dermatology, Saiseikai Tondabayashi Hospital, Osaka, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: Division of Dermatology, Saiseikai Tondabayashi Hospital, Osaka, Japan.
authors.forename: Aya
authors.initials: A
authors.lastname: Okabayashi
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
authors.forename: Chiharu
authors.initials: C
authors.lastname: Tateishi
authors.affiliation: Division of Surgery, Saiseikai Tondabayashi Hospital, Osaka, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Ikushima
authors.affiliation: Division of Pathology, Saiseikai Tondabayashi Hospital, Osaka, Japan.
authors.forename: Ren
authors.initials: R
authors.lastname: Nagasako
authors.affiliation: Division of Dermatology, Saiseikai Tondabayashi Hospital, Osaka, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: Department of Dermatology, Osaka City University Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsuruta
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005350:Fibroma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2
keywords: Basic FGF; Dermatofibroma; Elastofibroma; Fibroblast; TGF-β1