title: 1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501.
abstract: Conjugative transfer of DNA represents the most important transmission pathway in terms of antibiotic resistance and virulence gene dissemination among bacteria. TraH is a putative transfer protein of the type IV secretion system (T4SS) encoded by the Gram-positive (G+) conjugative plasmid pIP501. This molecular machine involves a multi-protein core complex spanning the bacterial envelope thereby serving as a macromolecular secretion channel. Here, we report the near complete (1)H, (13)C and (15)N resonance assignment of a soluble TraH variant comprising the C-terminal domain.
authors.affiliation: Institute for Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Fercher
authors.affiliation: Institute for Molecular Biosciences, University of Graz, Humboldtstraße 50/III, 8010, Graz, Austria.
authors.forename: Walter
authors.initials: W
authors.lastname: Keller
authors.affiliation: Institute of Chemistry, University of Graz, Heinrichstraße 28, 8010, Graz, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Zangger
authors.affiliation: General and Visceral Surgery, Department of Human Medicine, University of Oldenburg, Carl-von-Ossietzky-Straße 9-11, 26129, Oldenburg, Germany. helge.meyer@uni-oldenburg.de.
authors.forename: N Helge
authors.identifier: http://orcid.org/0000-0003-1018-4942
authors.initials: NH
authors.lastname: Meyer
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D013293:Enterococcus faecalis; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial
keywords: Antibiotic resistance dissemination; Backbone NMR resonance assignment; Bitopic transfer protein TraHpIP501; Clinically relevant Enterococcus faecalis; Gram-positive conjugation