title: Cytotoxic Orbitide from the Latex of Croton urucurana.
abstract: The bioactive ethyl acetate phase obtained from the latex of Croton urucurana Baillon afforded a novel orbitide (1), [1-9-NαC]-crourorb A1, that proved active against NCI-ADR/RES (ovary, multidrug-resistance phenotype) cells with the same potency as doxorubicin (positive control) and inactive up to the highest concentration tested against nontumor NIH/3T3 cells. The structure elucidation was based on 1D and 2D NMR spectroscopy, further supported by HRESIMS data and by application of Marfey's method for determination of the absolute configuration of its amino acid residues. This is the first orbitide obtained from C. urucurana.
authors.affiliation: Instituto de Química, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79074-460, Brazil.|Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79070-900, Brazil.
authors.forename: Priscila de M
authors.initials: Pde M
authors.lastname: Cândido-Bacani
authors.affiliation: Instituto de Química, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79074-460, Brazil.
authors.forename: Patrícia de O
authors.initials: Pde O
authors.lastname: Figueiredo
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79070-900, Brazil.
authors.forename: Maria de F C
authors.initials: Mde F
authors.lastname: Matos
authors.affiliation: Instituto de Química, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79074-460, Brazil.
authors.forename: Fernanda R
authors.initials: FR
authors.lastname: Garcez
authors.affiliation: Instituto de Química, Universidade Federal de Mato Grosso do Sul , Campo Grande, MS 79074-460, Brazil.
authors.forename: Walmir S
authors.initials: WS
authors.lastname: Garcez
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001938:Brazil; D029802:Croton / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007840:Latex / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D007840:Latex; D010456:Peptides, Cyclic; D010936:Plant Extracts