title: A silver complex of hyaluronan-lipoate (SHLS12): Synthesis, characterization and biological properties.
abstract: In this study we present a novel silver complex of hyaluronan-lipoate (SHLS12) in a gel-state form. NMR analysis, conductometry and elemental analysis demonstrated stable non-covalent interactions between silver ions and the polysaccharide-lipoate backbone, whereas rheological investigations confirmed its gel-like physical-chemical behavior. Biological studies showed the ability of SHLS12 to exert a straightforward activity against different bacterial strains grown in sessile/planktonic state. The biocompatibility was also proved toward two eukaryotic cell lines. By considering both its ability to preserve antibacterial properties when exposed to the serum protein BSA and its low susceptibility to be degraded by hyaluronidase enzyme, this novel complex may be considered as a promising biomaterial for future in vivo applications.
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Sacco
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sechi
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Trevisan
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Picotti
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Gianni
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Stucchi
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Fabbian
authors.affiliation: SIGEA Srl, AREA Science Park, loc. Padriciano 99, I-34149 Trieste, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Bosco
authors.affiliation: Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, I-34127 Trieste, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Paoletti
authors.affiliation: Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell'Ospitale 1, I-34129 Trieste, Italy. Electronic address: emarsich@units.it.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Marsich
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D012834:Silver / Q000737:chemistry; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005782:Gels; C000610709:SHLS12 oligosaccharide; D012834:Silver; D008063:Thioctic Acid; D006820:Hyaluronic Acid
keywords: Bacteria barrier effect; Gel; Hyaluronan; Hyaluronan–lipoate; Silver complex