title: Minocycline inhibits peritoneal macrophages but activates alveolar macrophages in acute pancreatitis.
abstract: Minocycline is a tetracycline antibiotic that, in addition to its antimicrobial function, has been reported to possess a relevant anti-inflammatory activity. Its effects have been extensively evaluated in inflammatory-related neurological diseases. Here, we evaluate its effect on the systemic inflammatory response in a model of experimental acute pancreatitis. Minocycline treatment significantly reduced the inflammation in pancreas and mesenterium, had no effect on the adipose tissue inflammation, and increased the inflammatory response in the lung. These differences seem to be related with different effects exerted on peritoneal and alveolar macrophages. In vitro, minocycline reduced the expression of IL-1β and inhibit the activation of nuclear factor kappa B (NF-κB) on peritoneal macrophages, while it had no effect on alveolar macrophages. Our data indicates that although minocycline may be useful as a tool to control some inflammatory processes, differences on its effects depending on the population of macrophages involved in the process can be expected. In the particular case of acute pancreatitis, it could promote or potentiate inflammation in the lung so that its use does not appear to be recommended.
authors.affiliation: Department of Experimental Pathology, IIBB-CSIC, IDIBAPS, c/Rosselló 161, 7°, Barcelona, 08036, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Bonjoch
authors.affiliation: Department of Experimental Pathology, IIBB-CSIC, IDIBAPS, c/Rosselló 161, 7°, Barcelona, 08036, Spain.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Gea-Sorlí
authors.affiliation: Departamento Ciencias Médicas, Facultad de Medicina, Universidad Castilla-La Mancha, Albacete, Spain.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Jordan
authors.affiliation: Department of Experimental Pathology, IIBB-CSIC, IDIBAPS, c/Rosselló 161, 7°, Barcelona, 08036, Spain. daniel.closa@iibb.csic.es.
authors.forename: Daniel
authors.initials: D
authors.lastname: Closa
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008911:Minocycline / Q000494:pharmacology*; D019283:Pancreatitis, Acute Necrotizing / Q000188:drug therapy* / Q000276:immunology; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D008911:Minocycline
keywords: Inflammation; Macrophages; Minocycline; Pancreatitis