title: A label-free fluorescent probe based on DNA-templated silver nanoclusters and exonuclease III-assisted recycling amplification detection of nucleic acid.
abstract: A number of specific nucleic acids are closely related with many serious diseases, in the current research, a platform taking advantage of exonuclease III (Exo III) to realize double recycling amplification and label-free fluorescent DNA-templated silver nanoclusters (DNA-AgNCs) for detecting of nucleic acid had been developed. In this method, a molecular beacon (MB) with 3'-protruding termini and a single-stranded cytosine-rich (C-rich) probe were designed that coexist stably with Exo III. Once the target DNA appeared, portion of the MB could hybridize with target DNA and was digested by Exo III, which allowed the release of target DNA and a residual sequence. Subsequently, the residual sequence could trigger the Exo III to digest C-rich probe, and the DNA-AgNCs was not able to be synthesized because of the C-rich probe was destroyed; finally the fluorescent of solution was quenched. This assay enables to monitor human hemochromatosis gene (as a model) with high sensitivity, the detection limit is as low as 120 pM compared with other fluorescence DNA-AgNCs methods, this assay also exhibits superior specificity even against single base mismatch. The strategy is applied to detect human hemochromatosis gene in real human serum samples successfully.
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: birdtjn@sina.com.
authors.forename: Jianniao
authors.initials: J
authors.lastname: Tian
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China. Electronic address: yanchunzhao@aliyun.com.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin 541004, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D004247:DNA / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures; D009696:Nucleic Acids / Q000032:analysis*; D012834:Silver / Q000737:chemistry*; D013698:Templates, Genetic
chemical_list: D005456:Fluorescent Dyes; D009696:Nucleic Acids; D012834:Silver; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III
keywords: DNA-templated silver nanoclusters; Exonuclease III; Fluorescence; Recycling amplification