title: Integrated production of cellulosic bioethanol and succinic acid from industrial hemp in a biorefinery concept.
abstract: The aim of this study was to develop integrated biofuel (cellulosic bioethanol) and biochemical (succinic acid) production from industrial hemp (Cannabis sativa L.) in a biorefinery concept. Two types of pretreatments were studied (dilute-acid and alkaline oxidative method). High cellulose recovery (>95%) as well as significant hemicelluloses solubilization (49-59%) after acid-based method and lignin solubilization (35-41%) after alkaline H2O2 method were registered. Alkaline pretreatment showed to be superior over the acid-based method with respect to the rate of enzymatic hydrolysis and ethanol productivity. With respect to succinic acid production, the highest productivity was obtained after liquid fraction fermentation originated from steam treatment with 1.5% of acid. The mass balance calculations clearly showed that 149kg of EtOH and 115kg of succinic acid can be obtained per 1ton of dry hemp. Results obtained in this study clearly document the potential of industrial hemp for a biorefinery.
authors.affiliation: Faculty of Materials, Civil and Environmental Engineering, University of Bielsko-Biala, Willowa 2, 43-309 Bielsko-Biala, Poland.
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kuglarz
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Merlin
authors.initials: M
authors.lastname: Alvarado-Morales
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Karakashev
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark. Electronic address: iria@env.dtu.dk.
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002188:Cannabis / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D019802:Succinic Acid / Q000378:metabolism*; D013997:Time Factors
chemical_list: D056804:Biofuels; D000431:Ethanol; D002482:Cellulose; D019802:Succinic Acid
keywords: Biorefinery; Cellulosic bioethanol; Industrial hemp; Pretreatment; Succinic acid