title: The Effect of Sleep Deprivation on Cardiac Function and Tolerance to Ischemia-Reperfusion Injury in Male Rats.
abstract: BACKGROUND
  Sleep deprivation (SD) is strongly associated with elevated risk for cardiovascular disease.
  
  
  OBJECTIVE
  To determine the effect of SD on basal hemodynamic functions and tolerance to myocardial ischemia-reperfusion (IR) injury in male rats.
  
  
  METHODS
  SD was induced by using the flowerpot method for 4 days. Isolated hearts were perfused with Langendorff setup, and the following parameters were measured at baseline and after IR: left ventricular developed pressure (LVDP); heart rate (HR); and the maximum rate of increase and decrease of left ventricular pressure (± dp/dt). Heart NOx level, infarct size and coronary flow CK-MB and LDH were measured after IR. Systolic blood pressure (SBP) was measured at start and end of study.
  
  
  RESULTS
  In the SD group, the baseline levels of LVDP (19%), +dp/dt (18%), and -dp/dt (21%) were significantly (p < 0.05) lower, and HR (32%) was significantly higher compared to the controls. After ischemia, hearts from SD group displayed a significant increase in HR together with a low hemodynamic function recovery compared to the controls. In the SD group, NOx level in heart, coronary flow CK-MB and LDH and infarct size significantly increased after IR; also SD rats had higher SBP after 4 days.
  
  
  CONCLUSIONS
  Hearts from SD rats had lower basal cardiac function and less tolerance to IR injury, which may be linked to an increase in NO production following IR.
authors.affiliation: Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Sajad
authors.initials: S
authors.lastname: Jeddi
authors.affiliation: Aerospace Medicine Research Center, Medical Faculty of Aerospace and subaquatic Medicine, AJA Medical Sciences University, Tehran, Iran.
authors.forename: Amir Nezami
authors.initials: AN
authors.lastname: Asl
authors.affiliation: Aerospace Medicine Research Center, Medical Faculty of Aerospace and subaquatic Medicine, AJA Medical Sciences University, Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Asgari
authors.affiliation: Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Ghasemi
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D052279:Creatine Kinase, MB Form / Q000032:analysis; D006321:Heart / Q000503:physiopathology*; D006439:Hemodynamics / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D009203:Myocardial Infarction; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D011897:Random Allocation; D017208:Rats, Wistar; D012016:Reference Values; D012307:Risk Factors; D012892:Sleep Deprivation / Q000378:metabolism / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form