title: Controllable Drug Release System in Living Cells Triggered by Enzyme-Substrate Recognition.
abstract: Vehicles can deliver the drug molecules into cells, yet immunoreaction of the commonly used capping agents and release triggers limit their biomedical use. This shortcoming might be circumvented through replacing these chemicals with certain biomolecules. Here, we show a new and facile way to encapsulate the drug delivery vehicles and release the cargos in a highly controllable manner via modulating supramolecular interactions between enzyme, substrate, and vehicle. The cargo release from the vehicles within cells can be achieved upon substrate treatment. Yeast cells were used, allowing for a fast and cost-effective way for imaging and morphological analysis. We believe this new platform can be readily extended to various carrier systems for different purposes based on shifting the recognition pattern of enzyme-substrate pairs.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University , 2699 Qianjin Street, Chaoyang, Changchun, Jilin 130012, People's Republic of China.
authors.forename: Pengchang
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University , 2699 Qianjin Street, Chaoyang, Changchun, Jilin 130012, People's Republic of China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University , 2699 Qianjin Street, Chaoyang, Changchun, Jilin 130012, People's Republic of China.|Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu , FI-90014 Oulu, Finland.
authors.forename: Kalervo
authors.initials: K
authors.lastname: Hiltunen
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University , 2699 Qianjin Street, Chaoyang, Changchun, Jilin 130012, People's Republic of China.|Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu , FI-90014 Oulu, Finland.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
mesh_terms: D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D050296:Microbial Viability; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D012235:Rhodamines / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D012822:Silicon Dioxide / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D003692:Delayed-Action Preparations; D012235:Rhodamines; C026188:rhodamine 6G; D012822:Silicon Dioxide; D005982:Glutathione Transferase; D005978:Glutathione
keywords: delivery; enzyme−substrate recognition; living cells; release control; yeast