title: Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking.
abstract: In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors' age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.
authors.affiliation: Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran Iran.
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
authors.affiliation: cGMP Grade Stem Cell Facility, Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Aghayan
authors.affiliation: School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Goodarzi
authors.affiliation: Department of Biology, Payame Noor University, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Mohamadi-Jahani
authors.affiliation: Brain and Spinal Cord Injury Research Center, Cellular Fanavaran Knowledge-Based Organization, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Norouzi-Javidan
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Dehpour
authors.affiliation: cGMP Grade Stem Cell Facility, Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Fallahzadeh
authors.affiliation: Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
authors.forename: Forough
authors.initials: F
authors.lastname: Azam Sayahpour
authors.affiliation: Department of Biology, Payame Noor University, Tehran, Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Bidaki
authors.affiliation: Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Babak
authors.initials: B
authors.lastname: Arjmand
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D018070:Biological Specimen Banks; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*
chemical_list: D003470:Culture Media
keywords: Adipose-derived stem cell; Cell therapy; Good manufacturing practice; Mesenchymal stem cells