title: Radiation enhancing effects of sanazole and gemcitabine in hypoxic breast and cervical cancer cells in vitro.
abstract: OBJECTIVE
  Sanazole and gemcitabine have been proven clinically as hypoxic cell radiosensitisers. This study was conducted to determine the radiation enhancing effects of sanazole and gemcitabine when administered together at relevant concentrations into hypoxic human MCF-7 and HeLa cells.
  
  
  METHODS
  A 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay was used to evaluate the number of surviving cells. Cell cycle was determined by flow cytometry. Cell surviving fractions were determined by the standard in vitro colony formation assay.
  
  
  RESULTS
  The cell colony formation assay indicated that the radiosensitivity of hypoxic MCF-7 and HeLa cells was enhanced by sanazole or gemcitabine. The combination of the two drugs displayed significant radiation enhancing effects at the irradiation doses of 6, 8, and 10 Gy in both cell lines, which were arrested in the S phase.
  
  
  CONCLUSIONS
  This study indicated that the co-administration of the two drugs may result in a beneficial gain in radio-therapy for hypoxic breast cancer and cervical cancer.
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China ; Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.
authors.forename: Yue-Can
authors.initials: YC
authors.lastname: Zeng
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
authors.affiliation: Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Xiao
authors.affiliation: Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chi
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Medical Oncology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xue
authors.affiliation: Cancer Insititute, No. 1 Hospital of China Medical University, Shenyang, China.
authors.forename: Nai-Qian
authors.initials: NQ
authors.lastname: Wang
keywords: HeLa cells; MCF-7 cells; Sanazole; gemcitabine; radiosensitiser