title: A portable optical waveguide resonance light-scattering scanner for microarray detection.
abstract: In the present work, a portable and low-cost planar waveguide based resonance light scattering (RLS) scanner (termed as: PW-RLS scanner) has been developed for microarray detection. The PW-RLS scanner employs a 2 × 4 white light emitting diode array (WLEDA) as the excitation light source, a folded optical path with a complementary metal oxide semiconductor (CMOS) as the signal/image acquisition device and stepper motors with gear drives as the mechanical drive system. The biological binding/recognizing events on the microarray can be detected with an evanescent waveguide-directed illumination and light-scattering label (e.g., nanoparticles) while the microarray slide acts as an evanescent waveguide substrate. The performance of the as-developed PW-RLS scanner has been evaluated by analyzing type 2 diabetes mellitus (T2DM) risk genes. Highly selective and sensitive (less than 1% allele frequency at the attomole-level) T2DM risk gene detection is achieved using single-stranded DNA functionalized gold nanoparticles (ssDNA-GNPs) as detection probes. Additionally, the successful simultaneous analysis of 15 T2DM patient genotypes suggests that the device has great potential for the realization of a personalized diagnostic test for a given disease or patient follow-up.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. litao@ciac.ac.cn wangzx@ciac.ac.cn.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Xing
authors.forename: Wanyao
authors.initials: W
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Shu
authors.initials: S
authors.lastname: Xing
authors.forename: Xueqi
authors.initials: X
authors.lastname: Fu
authors.forename: Dongyang
authors.initials: D
authors.lastname: Wu
authors.forename: Dianjun
authors.initials: D
authors.lastname: Liu
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D004867:Equipment Design; D008027:Light; D008670:Metals / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D055096:Optical Devices; D010087:Oxides / Q000737:chemistry; D012542:Scattering, Radiation; D012666:Semiconductors
chemical_list: D008670:Metals; D010087:Oxides