title: Synergistic enhancement of bone regeneration by LMP-1 and HIF-1α delivered by adipose derived stem cells.
abstract: OBJECTIVE
  To investigate the effect of the combination of LMP-1 and HIF-1α delivered by adipose-derived stem cells (ADSCs) on osteogenesis in vitro and in vivo.
  
  
  RESULTS
  Cells expressing both LMP-1 and HIF-1α genes had elevated mRNA expression of BMP-2, RunX2, alkaline phosphatase, osteocalcin, collagen I and alkaline phosphatase activity compared to cells from other groups. Furthermore, mineralization at day 14 in the cells expressing both LMP-1 and HIF-1α was significantly higher than in all the other groups. In vivo, H&E staining and immunohistochemical analysis of the cell-scaffolds also showed more ectopic bone formation at 4 weeks compared to other groups. More new vessel formation was apparent in the pLVX-rHIF-1α and pLVX-rLMP-1-rHIF-1α groups.
  
  
  CONCLUSIONS
  LMP-1 and HIF-1α gene delivery synergistically enhanced the osteo-differentiation of ADSCs in vitro and promoted osteogenesis in vivo compared with LMP-1 alone or HIF-1α alone.
authors.affiliation: Department of Human Movement Studies, Xi'an Physical Education University, Xi'an City, Shaanxi Province, 710068, People's Republic of China. panweiminpanda@hotmail.com.
authors.forename: Pan
authors.initials: P
authors.lastname: Weimin
authors.affiliation: Departments of Orthopaedics, The First Affiliated Hospital of China People's Liberation Army General Hospital, Beijing, 100037, People's Republic of China. caozheng_81@163.com.
authors.forename: Cao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Orthopaedics, Hospital of Armed Police Force of Shaanxi, Xi'an City, Shaanxi Province, 710054, People's Republic of China. shuaijun9500@hotmail.com.
authors.forename: Jia
authors.initials: J
authors.lastname: Shuaijun
authors.affiliation: Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an City, Shaanxi Province, 710032, People's Republic of China. bonebank@fmmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Dan
authors.affiliation: Department of Human Movement Studies, Xi'an Physical Education University, Xi'an City, Shaanxi Province, 710068, People's Republic of China. 88409727@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jianchang
authors.affiliation: Department of Human Movement Studies, Xi'an Physical Education University, Xi'an City, Shaanxi Province, 710068, People's Republic of China. 1463189872@qq.com.
authors.forename: Huang
authors.initials: H
authors.lastname: Yue
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006651:Histocytochemistry; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D060588:LIM Domain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects; D013234:Stem Cells / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D060588:LIM Domain Proteins; C484380:PDLIM7 protein, human
keywords: Adipose-derived stem cells; Gene transfer; Hypoxia-inducible factor-1α; LIM mineralization protein-1; Osteogenesis