title: Evaluation of Human Semenogelin Membrane Strip Test for Species Cross-reactivity in Dogs.
abstract: Semenogelins are proteins originating in the seminal vesicle and are useful markers for the presumptive identification of human semen. Detection of semenogelin can be done with a commercially available membrane test. In this study, a commercially available membrane test for human semenogelin proteins was used to assess for cross-reactivity in dog bodily fluids to allow for the potential utilization for detection of human semen in dog bodily fluids. The authors analyzed canine semen and other bodily fluids, including urine, saliva, vaginal secretions, fecal material, and blood. They also examined the distribution of human semenogelin I transcripts in the canine testis, prostate, and several bodily fluids by reverse transcription polymerase chain reaction. No cross-reactivity was observed in the canine bodily fluids tested except for a single rectal swab, which was negative on a second test. Further testing should be done to validate the use of this kit for screening samples from dogs suspected to have been victims of sexual abuse.
authors.affiliation: Veterinary Diagnostic Laboratory, University of Illinois, Urbana, IL, USA awstern@illinois.edu.
authors.forename: A W
authors.initials: AW
authors.lastname: Stern
authors.affiliation: Veterinary Diagnostic Laboratory, University of Illinois, Urbana, IL, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lanka
mesh_terms: D000827:Animal Welfare; D000818:Animals; D001769:Blood / Q000276:immunology; D012634:Bodily Secretions / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004285:Dogs; D005243:Feces; D005260:Female; D006801:Humans; D008297:Male; D010262:Paraphilic Disorders / Q000175:diagnosis; D011934:Reagent Strips; D012463:Saliva / Q000276:immunology; D012661:Semen / Q000276:immunology*; D029607:Seminal Vesicle Secretory Proteins / Q000276:immunology*; D014556:Urine; D014621:Vagina / Q000378:metabolism
chemical_list: D011934:Reagent Strips; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen
keywords: animal cruelty; dogs; forensic science; forensic serology; humans; semen; semenogelin protein; sexual abuse