title: Osteogenic differentiation of CD271(+) cells from rabbit bone marrow cultured on three phase PCL/TZ-HA bioactive scaffolds: comparative study with mesenchymal stem cells (MSCs).
abstract: Tissue engineering is one of the major challenges of orthopedics and trauma surgery for bone regeneration. Biomaterials filled with mesenchymal stem cells (MSCs) are considered the most promising approach in bone tissue engineering. Furthermore, our previous study showed that the multi-phase poly [ε-caprolactone]/thermoplastic zein-hydroxyapatite (PCL/TZ-HA) biomaterials improved rabbit (r) MSCs adhesion and osteoblast differentiation, thus demonstrating high potential of this bioengineered scaffold for bone regeneration. In the recent past, CD271 has been applied as a specific selective marker for the enrichment of MSCs from bone marrow (BM-MSCs). In the present study, we aimed at establishing whether CD271-based enrichment could be an efficient method for the selection of rBM-MSCs, displaying higher ability in osteogenic differentiation than non-selected rBM-MSCs in an in vitro system. CD271(+) cells were isolated from rabbit bone marrow and were compared with rMSCs in their proliferation rate and osteogenic differentiation capability. Furthermore, rCD271(+) cells were tested in their ability to adhere, proliferate and differentiate into osteogenic lineage, while growing on PCL/TZ-HA scaffolds, in comparison to rMSCs. Our result demonstrate that rCD271(+) cells were able to adhere, proliferate and differentiate into osteoblasts when cultured on PCL/TZ-HA scaffolds in significantly higher levels as compared to rMSCs. Based on these findings, CD271 marker might serve as an optimal alternative MSCs selection method for the potential preclinical and clinical application of these cells in bone tissue regeneration.
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Colosimo
authors.affiliation: Research Laboratory, "Stem Cells", U.O.C. Immunohematology and Transfusion Medicine, Hospital Santo Spirito Pescara, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rofani
authors.affiliation: Research Laboratory, "Stem Cells", U.O.C. Immunohematology and Transfusion Medicine, Hospital Santo Spirito Pescara, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Ciraci
authors.affiliation: Interdisciplinary Research Centre on Biomaterials (CRIB) and Italian Institute of Technology (IIT) Naples, Italy.
authors.forename: Aurelio
authors.initials: A
authors.lastname: Salerno
authors.affiliation: Department of Materials and Production Engineering, University of Naples Federico II Naples, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Oliviero
authors.affiliation: Department of Materials and Production Engineering, University of Naples Federico II Naples, Italy.
authors.forename: Ernesto Di
authors.initials: ED
authors.lastname: Maio
authors.affiliation: Institute of Composite and Biomedical Materials, National Research Council (IMCB-CNR) Naples, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Iannace
authors.affiliation: Interdisciplinary Research Centre on Biomaterials (CRIB) and Italian Institute of Technology (IIT) Naples, Italy.
authors.forename: Paolo A
authors.initials: PA
authors.lastname: Netti
authors.affiliation: Neurosurgery, Bambino Gesù Children's Hospital, Scientific Institute (IRCSS) Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Velardi
authors.affiliation: Research Laboratory, "Stem Cells", U.O.C. Immunohematology and Transfusion Medicine, Hospital Santo Spirito Pescara, Italy.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Berardi
keywords: CD271; Rabbit bone marrow; biomaterials; bone tissue engineering; mesenchymal stem cells; osteoblast