title: Genotoxicity and Cytotoxicity Evaluation of the Neolignan Analogue 2-(4-Nitrophenoxy)-1Phenylethanone and its Protective Effect Against DNA Damage.
abstract: Neolignans are secondary metabolites found in various groups of Angiosperms. They belong to a class of natural compounds with great diversity of chemical structures and pharmacological activities. These compounds are formed by linking two phenylpropanoid units. Several compounds that have ability to prevent genetic damage have been isolated from plants, and can be used to prevent or delay the development of tumor cells. Genetic toxicology evaluation is widely used in risk assessment of new drugs in preclinical screening tests. In this study, we evaluated the genotoxicity and cytotoxicity of the neolignan analogue 2-(4-nitrophenoxy)-1-phenylethanone (4NF) and its protective effect against DNA damage using the mouse bone marrow micronucleus test and the comet assay in mouse peripheral blood. Our results showed that this neolignan analogue had no genotoxic activity and was able to reduce induced damage both in mouse bone marrow and peripheral blood. Although the neolignan analogue 4NF was cytotoxic, it reduced cyclophosphamide-induced cytotoxicity. In conclusion, it showed no genotoxic action, but exhibited cytotoxic, antigenotoxic, and anticytotoxic activities.
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Alex Lucas
authors.initials: AL
authors.lastname: Hanusch
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Guilherme Roberto de
authors.initials: GR
authors.lastname: Oliveira
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Simone Maria Teixeira de
authors.initials: SM
authors.lastname: Sabóia-Morais
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Rafael Cosme
authors.initials: RC
authors.lastname: Machado
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Murilo Machado
authors.initials: MM
authors.lastname: Dos Anjos
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Lee
authors.initials: L
authors.lastname: Chen Chen
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D000818:Animals; D015197:Carcinogenicity Tests; D004249:DNA Damage / Q000187:drug effects*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests
chemical_list: C000608432:2-(4-nitrophenoxy)-1-phenylethanone; D000098:Acetophenones