title: Immunomodulation of Lactobacillus reuteri CRL1324 on Group B Streptococcus Vaginal Colonization in a Murine Experimental Model.
abstract: OBJECTIVE
  Maternal Group B Streptococcus (GBS) colonization is a risk factor for infectious disease in newborns. One promising strategy is the modulation of vaginal defense to increase the host's ability to combat infection.
  
  
  METHODS
  The effect of intravaginal (i.va.) Lactobacillus reuteri CRL1324 inoculation on different immune cell populations, cytokines, and immunoglobulin isotypes in a murine model of GBS vaginal colonization was evaluated.
  
  
  RESULTS
  Seven i.va. inoculations of L. reuteri CRL1324 previous to GBS challenge showed an immunomodulatory effect on the cells and mediators of innate immunity, decreasing the number of neutrophils induced by the pathogen and increasing the activated macrophage population. Moreover, increases in B lymphocytes and IgA and IgG subclasses were observed in mice inoculated with L. reuteri CRL1324 and then challenged with GBS.
  
  
  CONCLUSIONS
  Lactobacillus reuteri CRL1324 shows a protective effect against GBS colonization that could be mediated by the modulation of the immune response.
authors.affiliation: Centro de Referencia Para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: Priscilla Romina
authors.initials: PR
authors.lastname: De Gregorio
authors.affiliation: Centro de Referencia Para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: María Silvina
authors.initials: MS
authors.lastname: Juárez Tomás
authors.affiliation: Centro de Referencia Para Lactobacilos (CERELA)-CONICET, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: María Elena Fátima
authors.initials: ME
authors.lastname: Nader-Macías
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D056747:Immunomodulation; D052200:Limosilactobacillus reuteri / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009504:Neutrophils / Q000276:immunology* / Q000382:microbiology; D013290:Streptococcal Infections / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*; D014621:Vagina / Q000276:immunology* / Q000382:microbiology
keywords: Group B Streptococci; Lactobacillus; immune cells; mice; preventive effect; vaginal tract