title: Serum miR-21 may be a Potential Diagnostic Biomarker for Diabetic Nephropathy.
abstract: MiRNAs play important roles in initiation and progress of many pathologic processes. MiR-21 was closely associated with diabetic nephropathy (DN). However, whether serum miR-21 was as a potential diagnostic biomarker for DN and the relationship between serum miR-21 and tissue miR-21 remained unclear. In this study, real-time RT-PCR, cell transfection, luciferase reporter gene assays, western blot and confocal microscope were used, respectively. Here, we found that serum and renal tissue miR-21 was significantly elevated with the progress of DN. Moreover, luciferase reporter gene assays showed that smad7 was a validated miR-21 target, cell transfection showed that miR-21 overexpression downregulated target smad7 expression. Interestingly, serum miR-21 was significantly consistent with the alterations of tissue miR-21 with the development of DN. In addition, serum miR-21 was also positively correlated with GBM, GA, ACR and CCF, while negatively correlated with Ccr. Importantly, antagomiR-21 not only alleviated GBM, GA, ACR and CCF, but also ameliorated Ccr by increasing target smad7. In conclusion, our data demonstrated that serum miR-21 was closely associated with renal structure and function, and serum miR-21 may be regarded as a potential diagnostic biomarker of DN.
authors.affiliation: Department of Endocrinology, Gansu Provincial People's Hospital, Donggang West Road, Lanzhou, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Gansu Provincial People's Hospital, Donggang West Road, Lanzhou, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Duan
authors.affiliation: Department of Endocrinology, Gansu Provincial People's Hospital, Donggang West Road, Lanzhou, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Endocrinology, Gansu Provincial People's Hospital, Donggang West Road, Lanzhou, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Clinical College of Ophthalmology, Tianjin Medical University, Tianjin Eye Hospital, Tianjin, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: Metabolic Disease Center, School of Traditional Chinese medical, Capital Medical University, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D018929:Cell Culture Techniques; D003928:Diabetic Nephropathies / Q000097:blood / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000097:blood / Q000378:metabolism*; D019459:Urothelium / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs