title: Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.
abstract: Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.
authors.affiliation: Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287, Darmstadt, Germany.
authors.forename: Marc
authors.initials: M
authors.lastname: Vogel
authors.affiliation: Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287, Darmstadt, Germany. bsuess@bio.tu-darmstadt.de.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Suess
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002151:Calorimetry / Q000379:methods; D066298:In Vitro Techniques; D009154:Mutation; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013816:Thermodynamics; D017186:Titrimetry / Q000379:methods; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D012313:RNA
keywords: Aptamer; ITC; In vitro transcription; K d; RNA; Small molecule