title: Genomic Characterization of Dehalococcoides mccartyi Strain JNA That Reductively Dechlorinates Tetrachloroethene and Polychlorinated Biphenyls.
abstract: Dehalococcoides mccartyi strain JNA detoxifies highly chlorinated polychlorinated biphenyl (PCB) mixtures via 85 distinct dechlorination reactions, suggesting that it has great potential for PCB bioremediation. However, its genomic and functional gene information remain unknown due to extremely slow growth of strain JNA with PCBs. In this study, we used tetracholorethene (PCE) as an alternative electron acceptor to grow sufficient biomass of strain JNA for subsequent genome sequencing and functional gene identification. Analysis of the assembled draft genome (1 462 509 bp) revealed the presence of 29 putative reductive dehalogenase (RDase) genes. Among them, JNA_RD8 and JNA_RD11 genes were highly transcribed in both PCE- and PCB-fed cultures. Furthermore, in vitro assays with crude cell lysate from PCE grown cells revealed dechlorination activity against both PCE and 2,2',3,4,4',5,5'-heptachlorobiphenyl. These data suggest that both JNA_RD8 and JNA_RD11 may be bifunctional PCE/PCB RDases. This study deepens the knowledge of organohalide respiration of PCBs and facilitates in situ PCB-bioremediation with strain JNA.
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore , Singapore 117576.
authors.forename: Shanquan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore , Singapore 117576.|Computational and Systems Biology, Genome Institute of Singapore , Singapore 138672.
authors.forename: Kern Rei
authors.initials: KR
authors.lastname: Chng
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore , Singapore 117576.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Biological Sciences, Rensselaer Polytechnic Institute , 110 Eighth St., Troy, New York 12180, United States.
authors.forename: Donna L
authors.initials: DL
authors.lastname: Bedard
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore , Singapore 117576.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: He
mesh_terms: D001673:Biodegradation, Environmental; D001681:Biological Assay; D041862:Chloroflexi / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D023281:Genomics; D054879:Halogenation; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D013750:Tetrachloroethylene / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C034559:2,3,4,5,3',4',5'-heptachlorobiphenyl; C410127:PCB 180; D011078:Polychlorinated Biphenyls; D013750:Tetrachloroethylene