title: Association between interleukin 8 receptor α gene (CXCR1) and mastitis in dairy cattle.
abstract: The innate immune response plays an important role in the course of bacterial infections. Innate immunity effectiveness relies on the expression of many genes, connected, among others, to the activity of neutrophils. Interleukin 8 (IL-8) receptor α, coded by the CXCR1 gene, is present on the neutrophil surface and binds pro-inflammatory IL-8 with high affinity. This is why the bovine CXCR1 gene carries a potential for use as a dairy cattle mastitis marker. To date, several studies on the CXCR1 polymorphism brought out contradictory results. The aim of this study was to analyse the association between two SNPs of the CXCR1 gene, which is potentially important for the protein function and animal phenotype for mastitis susceptibility. A total of 554 Polish Holsteins were genotyped, and 140 among them were bacteriologically tested. The differences between animals carrying different genotypes and haplotypes of CXCR1 in test day somatic cell count (SCC) and Staphylococcus aureus mastitis susceptibility were estimated. We found that test day SCC was significantly related to CXCR1+472 SNP but not to CXCR1+735 SNP. No statistically significant association between CXCR1 polymorphism and susceptibility to S. aureus mastitis was found in the studied herd.
authors.affiliation: Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Magdalenka, Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Pawlik
authors.affiliation: Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Magdalenka, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Sender
authors.affiliation: Student at Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kapera
authors.affiliation: Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Magdalenka, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Korwin-Kossakowska
keywords: S. aureus; bovine CXCR1; mastitis; somatic cell count