title: Comparative Protein Profiling of Intraphagosomal Expressed Proteins of Mycobacterium bovis BCG.
abstract: BCG, the only available vaccine against tuberculosis affords a variable protection which wanes with time. In this study we have analyzed and compared the proteins which are expressed differentially during broth-culture and intraphagosomal growth of M.bovis BCG. Eight proteins which showed increased expression during the intraphagosomal growth were identified by MALDI-TOF/MS. These were - a precursor of alanine and proline-rich secreted protein apa, isoforms of malate dehydrogenase, large subunit alpha (Alpha-ETF) of electron transfer flavoprotein, immunogenic protein MPB64 precursor, UPF0036 protein, and two proteins with unknown function. Based on these findings we speculate that higher expression of these proteins has a probable role in intracellular survival, adaptation and/or immunoprotective effect of BCG. Further, these proteins might also be used as gene expression markers for endosome trafficking events of BCG.
authors.forename: Neelja
authors.initials: N
authors.lastname: Singhal
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Divakar
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra 282004, India. abd1109@rediffmail.com.
authors.forename: Deepa
authors.initials: D
authors.lastname: Bisht
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000382:microbiology*; D013058:Mass Spectrometry; D009163:Mycobacterium bovis / Q000254:growth & development* / Q000378:metabolism; D010588:Phagosomes / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome