title: [The influence of zoledronic acid on vascular endothelial cell].
abstract: OBJECTIVE
  To investigate the influence of zoledronic acid on vascular endothelial cells.
  
  
  METHODS
  The influence of zoledronic acid on proliferation, migration and adhesion of vascular endothelial cells were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), cell migration assay and cell adhesion assay. The results of each experimental group were compared with the control group and the data statistically analyzed.
  
  
  RESULTS
  In a concentration of 0-0.5 mmol/L, the absorbance value decreased from 0.09 to 0.34 as the drug concentration increased. Scratch test showed that the change of width of scratch before and after 24 hours in control, low, medium and high concentration groups were (38.7 ± 0.42), (35.8 ± 4.17), (19.9 ± 0.57) mm (P < 0.001), (12.5 ± 3.89) mm (P < 0.05). Adhesion test showed that the absorbance value in control, low, medium and high concentration groups were 1.14 ± 0.18, 0.95 ± 0.13, 0.81 ± 0.11 (P < 0.01), 0.67 ± 0.19 (P < 0.001). Comparisons between control and experimental groups were analyzed by t-test and P values < 0.05 were considered statistically significant.
  
  
  CONCLUSIONS
  Zoledronic acid inhibits the proliferation, migration and adhesion of vascular endothelial cells.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China.
authors.forename: Miaojie
authors.initials: M
authors.lastname: Lang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Mao
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Ren
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Oral and Maxillofacial Surgery, School & Hospital of Stomatology, Wenzhou Medical University, Wenzhou Zhejiang 325027, China; Email: zzh814490194@126.com.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Wang
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D004164:Diphosphonates / Q000493:pharmacokinetics / Q000494:pharmacology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D007093:Imidazoles / Q000493:pharmacokinetics / Q000494:pharmacology*; D000077211:Zoledronic Acid
chemical_list: D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid