title: Eribulin upregulates miR-195 expression and downregulates Wnt3a expression in non-basal-like type of triple-negative breast cancer cell MDA-MB-231.
abstract: Triple-negative breast cancer (TNBC), which does not show hormone sensitivity, is a poor prognosis disease without an established targeted treatment, so that establishing a therapeutic target for each subtype is desired. In addition, microRNA (miRNA), a non-cording RNA 19-25 nucleotide-longs in length, is known to be involved in regulating gene expression. We examined miRNA expression after exposure to eribulin, MDA-MB-231 cells, non-basal-like type of TNBC cell lines, and HCC1143 cells, basal-like type of TNBC cell lines. The activity of caspase-3 significantly increased compared to the control in MDA-MB-231, whereas no significant difference was observed in HCC1143. The expression level of 20-miRNAs significantly increased compared to the control in MDA-MB-231 after exposure to eribulin. The expression level of 6-miRNAs also significantly increased compared to the control in HCC1143. In these 2 cell types, miR-125b-1 and miR-195 were commonly expressed. While the expression level of miR-125b-1 decreased in both cells, the expression level of miR-195 increased in MDA-MB-231 and decreased in HCC1143. The expression level of miR-195 targeting Wnt3a significantly decreased compared to the control in MDA-MB-231, whereas it significantly increased in HCC1143. These results showed that exposure to eribulin highly increased the expression of miR-195 while it decreased the expression of Wnt3a in non-basal-like type of TNBC. Some miRNAs are known to regulate other signaling pathways involved in human pathogenesis by regulating the Wnt signaling pathway, and miRNA can act as a tumor-suppressing gene; therefore, miR-195 may serve as a therapeutic target in non-basal-like type of TNBC.
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan. k.ayuruf.5@gmail.com.
authors.forename: Kanji
authors.initials: K
authors.lastname: Furuya
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: Kameda Medical Center, Breast Center, 929 Higashi-cho, Kamogawa, Chiba, 296-8602, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsunoda
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tsuji
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Udaka
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Hideto
authors.initials: H
authors.lastname: Oyamada
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Tsuchiya
authors.affiliation: Department of Pharmacology, Showa University School of Medicine, Hatanodai 1-5-8, Shinagawaku, Tokyo, 142-8555, Japan.
authors.forename: Katsuji
authors.initials: K
authors.lastname: Oguchi
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D005663:Furans / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects*; D016147:Genes, Tumor Suppressor; D006801:Humans; D007659:Ketones / Q000494:pharmacology* / Q000627:therapeutic use; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D058990:Molecular Targeted Therapy; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects; D060509:Wnt3A Protein / Q000235:genetics* / Q000378:metabolism; D000095662:Polyether Polyketides
chemical_list: D005663:Furans; D007659:Ketones; D035683:MicroRNAs; D060509:Wnt3A Protein; C552722:MIRN195 microRNA, human; C555510:WNT3A protein, human; C490954:eribulin; D000095662:Polyether Polyketides
keywords: Breast cancer; Eribulin; MicroRNA; Triple negative; Wnt3a