title: The Tetrahymena telomerase p75-p45-p19 subcomplex is a unique CST complex.
abstract: Tetrahymena telomerase holoenzyme subunits p75, p45 and p19 form a subcomplex (7-4-1) peripheral to the catalytic core. We report structures of p45 and p19 and reveal them as the Stn1 and Ten1 subunits of the CST complex, which stimulates telomerase complementary-strand synthesis. 7-4-1 binds telomeric single-stranded DNA, and mutant p19 overexpression causes telomere 3'-overhang elongation. We propose that telomerase-tethered Tetrahymena CST coordinates telomere G-strand and C-strand synthesis.
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wan
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.|School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Tang
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Heather
authors.initials: H
authors.lastname: Upton
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shuai
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yuanzhe
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Northwestern University Center for Synchrotron Research, Life Sciences Collaborative Access Team, Argonne, Illinois, USA.
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Brunzelle
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Collins
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Center for Protein Science Shanghai, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.|Shanghai Science Research Center, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
mesh_terms: D008958:Models, Molecular; D011487:Protein Conformation; D055503:Protein Multimerization; D021122:Protein Subunits / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism; D013768:Tetrahymena / Q000201:enzymology*
chemical_list: D021122:Protein Subunits; D019098:Telomerase