title: Differences between calcium-stimulated and storage-induced erythrocyte-derived microvesicles.
abstract: Microvesicles (MVs), or microparticles, are a complex, dynamic and functional part of cells. Red blood cell (RBC)-derived MVs are naturally produced in vivo (during normal aging processes or in several diseases) as well as ex vivo during cold storage of RBCs, or in vitro by ATP depletion or treatment with Ca(2+) and calcium ionophore. All these MVs are equivalently classified according to their size and/or surface markers. Nevertheless, their content in proteins can differ and a few differences in terms of lipid raft proteins, notably stomatin and flotillin-2, have been reported. Based on two-dimensional gel electrophoreses, the present study highlights the differences between MVs induced during storage of RBCs (storage-MVs) and MVs stimulated by Ca(2+) entry (Ca-MVs). Upon treatment, Ca-MVs are formed following a clear recruitment of Ca(2+)-binding proteins (sorcin, grancalcin, PDCD6) and particularly annexins (4 and 5). Therefore, it emerges that different molecular pathways are available to produce similar MVs by disturbing the membrane/cytoskeleton interactions. Interestingly, these differences provide non-negligible pieces of information on the parent cells, and the mechanisms and modes of actions involved in the formation of MVs. In addition to biophysical characterization, protein analysis is important to classify these cellular corpuscles and evaluate their potential impacts in diseases or transfusion medicine.
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Prudent
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Crettaz
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Delobel
authors.affiliation: International Consultancy in Blood Components Quality/Safety, Audit/Inspection and DDR Strategy, London, UK.
authors.forename: Jerard
authors.initials: J
authors.lastname: Seghatchian
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Tissot
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland. Electronic address: niels.lion@itransfusion.ch.
authors.forename: Niels
authors.initials: N
authors.lastname: Lion
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001793:Blood Preservation; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D061207:Calcium Ionophores / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D055252:Cell-Derived Microparticles; D003599:Cytoskeleton / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male
chemical_list: D061207:Calcium Ionophores; D002135:Calcium-Binding Proteins; D000255:Adenosine Triphosphate; D002118:Calcium
keywords: Calcium; Erythrocyte; Microvesicle; Proteomics; Storage; Transfusion