title: Synthesis and characterization of starch-poly(methyl acrylate) graft copolymers using horseradish peroxidase.
abstract: Horseradish peroxidase (HRP)-mediated graft polymerization in the presence of hydrogen peroxide (H2O2) and acetylacetone (Acac) has been successfully applied to the synthesis of starch-poly(methyl acrylate) (PMA). The graft copolymer was characterized by Fourier transform infrared (FT-IR), elemental analysis, nuclear magnetic resonance ((1)H NMR and (13)C NMR), and differential scanning calorimetry (DSC). FT-IR, elemental analysis and NMR confirmed that methyl acrylate (MA) was grafted onto starch successfully. DSC results showed the graft reaction had changed the crystalline regions of the gelatinized starch. The effects of pH, MA content, HRP dosage, incubation temperature and time on grafting percentage (GP) and grafting efficiency (GE) were also investigated. The GP and GE under optimal conditions reached 30.21% and 45.13%, respectively.
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Su
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China; International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China; International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China. Electronic address: wxfxr@163.com.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Fan
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Jiugang
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi 214122, China.
authors.forename: Heling
authors.initials: H
authors.lastname: Jin
authors.affiliation: International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China; Center of Biological Engineering, University of Minho, Braga 4710-057, Portugal.
authors.forename: Artur
authors.initials: A
authors.lastname: Cavaco-Paulo
mesh_terms: D005782:Gels / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013213:Starch / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D005782:Gels; D013213:Starch; D019904:Polymethyl Methacrylate; D006735:Horseradish Peroxidase
keywords: Graft polymerization; Horseradish peroxidase; Methyl acrylate; Starch