title: Protective Effect of Eburicoic Acid of the Chicken of the Woods Mushroom, Laetiporus sulphureus (Higher Basidiomycetes), Against Gastric Ulcers in Mice.
abstract: In this study, we investigated the anti-inflammatory and tumor-inhibiting effects of eburicoic acid, the main bioactive component in the Laetiporus sulphureus, on gastric ulcers. A total of 48 Kunming mice were randomly divided into six groups: control, model, OL (omeprazole, 20 mg/kg/day, orally), EA-L (eburicoic acid, 10 mg/kg/day, orally), EA-M (eburicoic acid, 20 mg/kg/day, orally), and EA-H (eburicoic acid, 40 mg/kg/day, orally). Gastric ulcers were induced in mice by administering 80% ethanol containing 15 mg/mL aspirin (10.0 mL/kg, i.g.) 4 hours after drug administration on day 5. The ulcer index and H+/K+-ATPase activity were evaluated in vivo. Computer-aided molecular docking simulated the interaction between eburicoic acid and H+/K+-ATPase. The results showed that the oral administration of eburicoic acid protected the gastric mucosa from gastric lesions morphologically and especially attenuated H+/K+-ATPase activity. The results of this study indicate that the gastric protective effect of eburicoic acid might inhibit gastric acid.
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Sun
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
authors.forename: Huajun
authors.initials: H
authors.lastname: Luo
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: He
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Weiqiao
authors.initials: W
authors.lastname: Deng
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zou
authors.affiliation: College of Science and Technology, China Three Gorges University, Yichang, China.
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, Medical College, China Three Gorges University, Yichang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.affiliation: Hubei Key Laboratory of Natural Products Research and Development, Medical College, China Three Gorges University, Yichang, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D055453:Coriolaceae / Q000737:chemistry*; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000627:therapeutic use; D008297:Male; D051379:Mice; D015394:Molecular Structure; D020011:Protective Agents / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000473:pathology / Q000517:prevention & control*
chemical_list: D000897:Anti-Ulcer Agents; D020011:Protective Agents; D007810:Lanosterol; C004867:eburicoic acid; D017506:H(+)-K(+)-Exchanging ATPase