title: Cytotoxic T lymphocyte antigen 4 decreases humoral and cellular immunity by adenovirus to enhance target GFP gene transfer in C57BL/6 mice.
abstract: Adenoviruses (Ad) are once potential and promising vectors for gene delivery, but the immunogenicity attenuates its transfer efficiency. Cytotoxic T lymphocyte antigen 4 (CTLA-4) can inhibit T cell immunity. Thus, we aimed to study the effect of CTLA-4 in the process of Ad-mediated gene transfer. The C57BL/6 mice were injected by Ad vectors at twice, and CTLA-4 was administrated after the first Ad injection. Then, the CD3(+)CD4(+) T cells and circulating levels of IL-2, IL-4, and anti-Ad IgG were decreased by CTLA-4, while Ad generated immune responses. The green fluorescence protein (GFP) expressions of tissues were enhanced by CTLA-4 till injection of Ad at twice. Our results indicate that CTLA-4 can inhibit humoral and cellular immunity by adenovirus generation to enhance GFP delivery, and provide a potential way to assist in Ad-mediated gene transfer.
authors.affiliation: a Laboratory of Molecular Immunology , State Key Laboratory of Genetic Engineering, Institute of Biomedical Sciences, School of Life Sciences, Fudan University , Shanghai , P.R. China.
authors.forename: Dou
authors.initials: D
authors.lastname: Bai
authors.affiliation: a Laboratory of Molecular Immunology , State Key Laboratory of Genetic Engineering, Institute of Biomedical Sciences, School of Life Sciences, Fudan University , Shanghai , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: a Laboratory of Molecular Immunology , State Key Laboratory of Genetic Engineering, Institute of Biomedical Sciences, School of Life Sciences, Fudan University , Shanghai , P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: a Laboratory of Molecular Immunology , State Key Laboratory of Genetic Engineering, Institute of Biomedical Sciences, School of Life Sciences, Fudan University , Shanghai , P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Long
authors.affiliation: a Laboratory of Molecular Immunology , State Key Laboratory of Genetic Engineering, Institute of Biomedical Sciences, School of Life Sciences, Fudan University , Shanghai , P.R. China.
authors.forename: Naishuo
authors.initials: N
authors.lastname: Zhu
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D060908:CTLA-4 Antigen / Q000008:administration & dosage*; D005260:Female; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000008:administration & dosage*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage* / Q000096:biosynthesis; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D056724:Immunity, Humoral / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D060908:CTLA-4 Antigen; D049452:Green Fluorescent Proteins
keywords: Adenovirus; CTLA-4; gene transfer; humoral and cellular immunity