title: Regulation of formation of volatile compounds of tea (Camellia sinensis) leaves by single light wavelength.
abstract: Regulation of plant growth and development by light wavelength has been extensively studied. Less attention has been paid to effect of light wavelength on formation of plant metabolites. The objective of this study was to investigate whether formation of volatiles in preharvest and postharvest tea (Camellia sinensis) leaves can be regulated by light wavelength. In the present study, in contrast to the natural light or dark treatment, blue light (470 nm) and red light (660 nm) significantly increased most endogenous volatiles including volatile fatty acid derivatives (VFADs), volatile phenylpropanoids/benzenoids (VPBs), and volatile terpenes (VTs) in the preharvest tea leaves. Furthermore, blue and red lights significantly up-regulated the expression levels of 9/13-lipoxygenases involved in VFADs formation, phenylalanine ammonialyase involved in VPBs formation, and terpene synthases involved in VTs formation. Single light wavelength had less remarkable influences on formation of volatiles in the postharvest leaves compared with the preharvest leaves. These results suggest that blue and red lights can be promising technology for remodeling the aroma of preharvest tea leaves. Furthermore, our study provided evidence that light wavelength can activate the expression of key genes involved in formation of plant volatiles for the first time.
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Fu
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.|University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
authors.forename: Yiyong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Mei
authors.affiliation: Tea Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry 1706-11 Kurasawa, Kikugawa 439-0002, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Katsuno
authors.affiliation: Tea Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry 1706-11 Kurasawa, Kikugawa 439-0002, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Guangdong Food and Drug Vocational College, Longdongbei Road 321, Tianhe District, Guangzhou 510520, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan.
authors.forename: Naoharu
authors.initials: N
authors.lastname: Watanabe
authors.affiliation: Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.|Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.|University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.
authors.forename: Ziyin
authors.initials: Z
authors.lastname: Yang
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects; D019075:Electrophoresis, Capillary; D008401:Gas Chromatography-Mass Spectrometry; D008027:Light; D019787:Linoleic Acid / Q000032:analysis; D058945:Lipoxygenases / Q000235:genetics / Q000378:metabolism; D010649:Phenylalanine / Q000032:analysis / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013729:Terpenes / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000032:analysis
chemical_list: D010940:Plant Proteins; D013729:Terpenes; D055549:Volatile Organic Compounds; D017962:alpha-Linolenic Acid; D010649:Phenylalanine; D019787:Linoleic Acid; D058945:Lipoxygenases; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase