title: Characterization of successional changes in bacterial community composition during bioremediation of used motor oil-contaminated soil in a boreal climate.
abstract: The widespread use of motor oil makes it a notable risk factor to cause scattered contamination in soil. The monitoring of microbial community dynamics can serve as a comprehensive tool to assess the ecological impact of contaminants and their disappearance in the ecosystem. Hence, a field study was conducted to monitor the ecological impact of used motor oil under different perennial cropping systems (fodder galega, brome grass, galega-brome grass mixture and bare fallow) in a boreal climate zone. Length heterogeneity PCR characterized a successional pattern in bacterial community following oil contamination over a four-year bioremediation period. Soil pH and electrical conductivity were associated with the shifts in bacterial community composition. Crops had no detectable effect on bacterial community composition or complexity. However, the legume fodder galega increased soil microbial biomass, expressed as soil total DNA. Oil contamination induced an abrupt change in bacterial community composition at the early stage, yet the effect did not last as long as the oil in soil. The successional variation in bacterial community composition can serve as a sensitive ecological indicator of oil contamination and remediation in situ.
authors.affiliation: Department of Environmental Sciences, PO Box 65 (Viikinkaari 2a), 00014, University of Helsinki, Finland. Electronic address: lijuan.yan@helsinki.fi.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Food and Environmental Sciences, PO Box 56 (Latokartanonkaari 11), 00014, University of Helsinki, Finland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Sinkko
authors.affiliation: Department of Environmental Sciences, PO Box 65 (Viikinkaari 2a), 00014, University of Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Penttinen
authors.affiliation: Department of Environmental Sciences, PO Box 65 (Viikinkaari 2a), 00014, University of Helsinki, Finland.
authors.forename: Kristina
authors.initials: K
authors.lastname: Lindström
mesh_terms: D001673:Biodegradation, Environmental; D002980:Climate; D064307:Microbiota; D010578:Petroleum / Q000032:analysis*; D059392:Petroleum Pollution; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants
keywords: Bacterial community; Bioremediation; Brome grass; Fodder galega; LH-PCR; Oil contamination; Soil