title: Biosynthetic Study on Antihypercholesterolemic Agent Phomoidride: General Biogenesis of Fungal Dimeric Anhydrides.
abstract: To elucidate the general biosynthetic pathway of fungal dimeric anhydrides, a gene cluster for the biosynthesis of the antihy-percholesterolemic agent phomoidride was identified by heterologous expression of candidate genes encoding the highly reducing polyketide synthase, alkylcitrate synthase (ACS), and alkylcitrate dehydratase (ACDH). An in vitro analysis of ACS and ACDH revealed that they give rise to anhydride monomers. Based on the established monomer biosynthesis, we propose a general biogenesis of dimeric anhydrides involving a single donor unit and four acceptor units.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Ryuya
authors.initials: R
authors.lastname: Fujii
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Matsu
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Minami
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Nagamine
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takeuchi
authors.affiliation: Graduate School of Agricultural Science, Tohoku University , Sendai 981-8555, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University , Sapporo 060-0810, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D000812:Anhydrides / Q000737:chemistry; D000924:Anticholesteremic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001230:Aspergillus / Q000378:metabolism*; D053898:Biosynthetic Pathways; D006836:Hydro-Lyases / Q000378:metabolism; D008299:Maleic Anhydrides / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D005810:Multigene Family; D009183:Mycotoxins / Q000378:metabolism; D048630:Polyketide Synthases / Q000378:metabolism*
chemical_list: D000812:Anhydrides; D000924:Anticholesteremic Agents; C104957:CP 263114; D008299:Maleic Anhydrides; D009183:Mycotoxins; C100297:rubratoxins; D048630:Polyketide Synthases; D006836:Hydro-Lyases