title: Thalassobius abyssisp. nov., a marine bacterium isolated from cold-seep sediment.
abstract: A novel marine bacterial strain, designated JAMH 043T, was isolated from cold-seep sediment in Sagami Bay, Japan. Cells were Gram-stain-negative, rod-shaped, non-motile and aerobic chemo-organotrophs. The isolate grew optimally at 25 °C, at pH 7.0-7.5 and with 3 % (w/v) NaCl. The major respiratory quinone was ubiquinone-10 (Q-10). The predominant fatty acid was C18 : 1ω7c. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated to members of the genusThalassobiusin the classAlphaproteobacteria, and 16S rRNA gene sequence similarity of the novel isolate with the type strain of its closest related species,Thalassobius aestuariiJC2049T, was 98.4 %. The DNA G+C content of the novel strain was 58.0 mol%. The hybridization values for DNA-DNA relatedness between strain JAMH 043Tand reference strains belonging to the genusThalassobiuswere less than 14.1 ± 2.2 %. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genusThalassobius, for which the nameThalassobius abyssisp. nov. is proposed. The type strain is JAMH 043T( = JCM 30900T= DSM 100673T).
authors.affiliation: Research and Development Center for Marine Biosciences,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061,Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nogi
authors.affiliation: Research and Development Center for Marine Biosciences,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061,Japan.
authors.forename: Kozue
authors.initials: K
authors.lastname: Mori
authors.affiliation: Department of Subsurface Geobiological Analysis and Research,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061,Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Makita
authors.affiliation: Research and Development Center for Marine Biosciences,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061,Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada