title: Transcription factor C/EBPβ promotes the transcription of the porcine GPR120 gene.
abstract: G protein-coupled receptor 120 (GPR120), an adipogenic receptor critical for the differentiation and maturation of adipocytes, plays an important role in controlling obesity in both humans and rodents and, thus, is an attractive target of obesity treatment studies. However, the mechanisms that regulate the expression of porcine GPR120 remain unclear. In this study, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) techniques were used to analyze and identify the binding of C/EBPβ (transcription factor CCAAT/enhancer binding protein beta) to the GPR120 promoter. C/EBPβ overexpression and RNA interference studies showed that C/EBPβ regulated GPR120 promoter activity and endogenous GPR120 expression. The binding site of C/EBPβ in the GPR120 promoter region from -101 to -87 was identified by promoter deletion analysis and site-directed mutagenesis. Overexpression of C/EBPβ increased endogenous GPR120 expression in pig kidney cells (PK). Furthermore, when endogenous C/EBPβ was knocked down, GPR120 mRNA and protein levels were decreased. The stimulatory effect of C/EBPβ on GPR120 transcription and its ability to bind the transcription factor-binding site were confirmed by luciferase, ChIP, and EMSA. Moreover, the mRNA and protein expression levels of C/EBPβ were induced by high fat diet feeding. Taken together, it can be concluded that C/EBPβ plays a vital role in regulating GPR120 transcription and suggests HFD-feeding induces GPR120 transcription by influencing C/EBPβ expression.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Kun
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Ji-Dan
authors.initials: JD
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Meng-Si
authors.initials: MS
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Tang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Deng
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China leiminggang@aliyun.com xiongyuanzhu2008@163.com.
authors.forename: Ming-Gang
authors.initials: MG
authors.lastname: Lei
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministryand Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of ChinaWuhan Institute of Animal Science and Veterinary MedicineWuhan Academy of gricultural Science and Technology, Wuhan, Hubei, People's Republic of China leiminggang@aliyun.com xiongyuanzhu2008@163.com.
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Xiong
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D043562:Receptors, G-Protein-Coupled
keywords: C/EBPβ; ChIP; GPR120; porcine