title: Comparative Proteome Analysis of hAT-MSCs Isolated from Chronic Renal Failure Patients with Differences in Their Bone Turnover Status.
abstract: The relationship between the stem cells and the bone turnover in uremic bone disease due to chronic renal failure (CRF) is not described. The aim of this study was to investigate the effect of bone turnover status on stem cell properties. To search for the presence of such link and shed some light on stem-cell relevant mechanisms of bone turnover, we carried out a study with mesenchymal stem cells. Tissue biopsies were taken from the abdominal subcutaneous adipose tissue of a CRF patient with secondary hyperparathyroidism with the high turnover bone disease. This patient underwent parathyroidectomy operation (PTX) and another sample was taken from this patient after PTX. A CRF patient with adynamic bone disease with low turnover and a healthy control were also included. Mesenchymal stem cells isolated from the subjects were analyzed using proteomic and molecular approaches. Except ALP activity, the bone turnover status did not affect common stem cell properties. However, detailed proteome analysis revealed the presence of regulated protein spots. A total of 32 protein spots were identified following 2D gel electrophoresis and MALDI-TOF/TOF analyzes. The identified proteins were classified into seven distinct groups and their potential relationship to bone turnover were discussed. Distinct protein expression patterns emerged in relation to the bone turnover status indicate a possible link between the stem cells and bone turnover in uremic bone disease due to CRF.
authors.affiliation: Department of Medical Biology/DEKART Proteomics Laboratory, Kocaeli University Medical School, Kocaeli, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kasap
authors.affiliation: Department of Internal Medicine, Nephrology, Kocaeli University Medical School, Kocaeli, Turkey.
authors.forename: Itır
authors.initials: I
authors.lastname: Yeğenağa
authors.affiliation: Department of Medical Biology/DEKART Proteomics Laboratory, Kocaeli University Medical School, Kocaeli, Turkey.
authors.forename: Gurler
authors.initials: G
authors.lastname: Akpinar
authors.affiliation: Department of Internal Medicine, Nephrology, Kocaeli University Medical School, Kocaeli, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tuncay
authors.affiliation: Department of Stem Cell, Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey.
authors.forename: Ayça
authors.initials: A
authors.lastname: Aksoy
authors.affiliation: Liv Hospital Center for Regenerative Medicine and Stem Cell Research and Manufacturing, İstanbul, Turkey.
authors.forename: Erdal
authors.initials: E
authors.lastname: Karaoz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001847:Bone Diseases / Q000150:complications / Q000175:diagnosis; D016723:Bone Remodeling; D002454:Cell Differentiation; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006962:Hyperparathyroidism, Secondary / Q000150:complications / Q000175:diagnosis / Q000601:surgery; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D016105:Parathyroidectomy; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D051436:Renal Insufficiency, Chronic / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D019098:Telomerase / Q000378:metabolism
chemical_list: D020543:Proteome; D019098:Telomerase; D000469:Alkaline Phosphatase