title: Effect of Human Adipose Tissue Mesenchymal Stem Cells on the Regeneration of Ovine Articular Cartilage.
abstract: Cell therapy is a promising approach to improve cartilage healing. Adipose tissue is an abundant and readily accessible cell source. Previous studies have demonstrated good cartilage repair results with adipose tissue mesenchymal stem cells in small animal experiments. This study aimed to examine these cells in a large animal model. Thirty knees of adult sheep were randomly allocated to three treatment groups: CELLS (scaffold seeded with human adipose tissue mesenchymal stem cells), SCAFFOLD (scaffold without cells), or EMPTY (untreated lesions). A partial thickness defect was created in the medial femoral condyle. After six months, the knees were examined according to an adaptation of the International Cartilage Repair Society (ICRS 1) score, in addition to a new Partial Thickness Model scale and the ICRS macroscopic score. All of the animals completed the follow-up period. The CELLS group presented with the highest ICRS 1 score (8.3 ± 3.1), followed by the SCAFFOLD group (5.6 ± 2.2) and the EMPTY group (5.2 ± 2.4) (p = 0.033). Other scores were not significantly different. These results suggest that human adipose tissue mesenchymal stem cells promoted satisfactory cartilage repair in the ovine model.
authors.affiliation: Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil. alessandrozorzi@uol.com.br.|Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil. alessandrozorzi@uol.com.br.
authors.forename: Alessandro R
authors.initials: AR
authors.lastname: Zorzi
authors.affiliation: Department of Pathology, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil. ingrid@fcm.unicamp.br.
authors.forename: Eliane M I
authors.initials: EM
authors.lastname: Amstalden
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos/SP 13566-590, Brazil. amplepis@iqsc.usp.br.
authors.forename: Ana Maria G
authors.initials: AM
authors.lastname: Plepis
authors.affiliation: Instituto de Química de São Carlos, Universidade de São Paulo (USP), São Carlos/SP 13566-590, Brazil. virginia@iqsc.usp.br.
authors.forename: Virginia C A
authors.initials: VC
authors.lastname: Martins
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil. mario.ferretti@einstein.br.
authors.forename: Mario
authors.initials: M
authors.lastname: Ferretti
authors.affiliation: Hospital Israelita Albert Einstein, São Paulo/SP 05652-900, Brazil. eliane.antonioli@einstein.br.
authors.forename: Eliane
authors.initials: E
authors.lastname: Antonioli
authors.affiliation: Hematology Hemotherapy Centre, Universidade de Campinas (UNICAMP), Campinas/SP 13083-878, Brazil. adrianassduarte@uol.com.br.
authors.forename: Adriana S S
authors.initials: AS
authors.lastname: Duarte
authors.affiliation: Hematology Hemotherapy Centre, Universidade de Campinas (UNICAMP), Campinas/SP 13083-878, Brazil. angela.luzo@gmail.com.
authors.forename: Angela C M
authors.initials: AC
authors.lastname: Luzo
authors.affiliation: Department of Orthopedic Surgery, Faculty of Medical Sciences, Universidade de Campinas (UNICAMP), Campinas/SP 13083-887, Brazil. jotamiran@yahoo.com.br.
authors.forename: João B
authors.initials: JB
authors.lastname: Miranda
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D002358:Cartilage, Articular / Q000293:injuries / Q000601:surgery; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology* / Q000276:immunology; D005260:Female; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D012038:Regeneration / Q000502:physiology*; D012756:Sheep; D013264:Stifle / Q000293:injuries / Q000601:surgery; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous; D016896:Treatment Outcome
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens
keywords: adult stem cell; biomaterials; cartilage; regenerative medicine; stem cell therapy; tissue engineering