title: [Effect of topically applied recombinant adenovirus of adiponectin on bone defect repair].
abstract: OBJECTIVE
  To investigate the effect of local administration of recombinant adenovirus of human adiponectin (hAPN) Ad-hAPN-EGFP on tibial defect repair of SD rats.
  
  
  METHODS
  Tibial defect (2 mm) models of 36 SD rats (72 sides) were randomly divided into three groups (A, B, and C; n=24). The three groups were injected with Ad-hAPN-EGFP, Ad-EGFP, and normal saline, respectively, during operation and the day after operation. A week after the operation, the expression of hAPN and osteogenesis-related factors were detected by real-time polymerase chain reaction. Three weeks after operation, the tibias were examined by micro-computed tomography, hematoxylin-eosin staining, and Masson staining to evaluate the restoration of bone defects.
  
  
  RESULTS
  1) The hAPN expression was detected in group A but not in groups B and C. Osteogenesis-related factors expression of group A was significantly higher than that of the other groups (P<0.05). 2) Osteogenesis (including bone mineral density, relative bone volume, trabecular number and trabecular thickness) in group A were more evident than those in groups B and C (P<0.05). No significant differences were found between groups B and C (P>0.05).
  
  
  CONCLUSIONS
  Local administration of recombinant adenovirus Ad-hAPN-EGFP may be an effective strategy to improve the restoration of bone defects in vivo.
authors.forename: Xue
authors.initials: X
authors.lastname: Lü
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.forename: Jiayang
authors.initials: J
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.forename: En
authors.initials: E
authors.lastname: Luo
mesh_terms: D000256:Adenoviridae; D052242:Adiponectin; D000818:Animals; D015519:Bone Density; D001842:Bone and Bones; D006801:Humans; D010012:Osteogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D055114:X-Ray Microtomography
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin