title: GsCML27, a Gene Encoding a Calcium-Binding Ef-Hand Protein from Glycine soja, Plays Differential Roles in Plant Responses to Bicarbonate, Salt and Osmotic Stresses.
abstract: Calcium, as the most widely accepted messenger, plays an important role in plant stress responses through calcium-dependent signaling pathways. The calmodulin-like family genes (CMLs) encode Ca2+ sensors and function in signaling transduction in response to environmental stimuli. However, until now, the function of plant CML proteins, especially soybean CMLs, is largely unknown. Here, we isolated a Glycine soja CML protein GsCML27, with four conserved EF-hands domains, and identified it as a calcium-binding protein through far-UV CD spectroscopy. We further found that expression of GsCML27 was induced by bicarbonate, salt and osmotic stresses. Interestingly, ectopic expression of GsCML27 in Arabidopsis enhanced plant tolerance to bicarbonate stress, but decreased the salt and osmotic tolerance during the seed germination and early growth stages. Furthermore, we found that ectopic expression of GsCML27 decreases salt tolerance through modifying both the cellular ionic (Na+, K+) content and the osmotic stress regulation. GsCML27 ectopic expression also decreased the expression levels of osmotic stress-responsive genes. Moreover, we also showed that GsCML27 localized in the whole cell, including cytoplasm, plasma membrane and nucleus in Arabidopsis protoplasts and onion epidermal cells, and displayed high expression in roots and embryos. Together, these data present evidence that GsCML27 as a Ca2+-binding EF-hand protein plays a role in plant responses to bicarbonate, salt and osmotic stresses.
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Agronomy College, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Huizi
authors.initials: H
authors.lastname: Duanmu
authors.affiliation: College of Life Science, Qingdao Agricultural University, Qingdao, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Jia
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, P.R. China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002147:Calmodulin / Q000235:genetics; D002942:Circular Dichroism; D020832:EF Hand Motifs / Q000235:genetics; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009997:Osmotic Pressure / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics; D017434:Protein Structure, Tertiary; D055049:Salt Tolerance / Q000235:genetics*; D020539:Sequence Analysis, Protein; D015398:Signal Transduction / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D010940:Plant Proteins; D012965:Sodium Chloride; D002118:Calcium