title: Manool, a Salvia officinalis diterpene, induces selective cytotoxicity in cancer cells.
abstract: Manool, a diterpene isolated from Salvia officinalis, was evaluated by the XTT colorimetric assay for cytotoxicity and selectivity against different cancer cell lines: B16F10 (murine melanoma), MCF-7 (human breast adenocarcinoma), HeLa (human cervical adenocarcinoma), HepG2 (human hepatocellular carcinoma), and MO59J, U343 and U251 (human glioblastoma). A normal cell line (V79, Chinese hamster lung fibroblasts) was used to compare the selectivity of the test substance. Manool exhibited higher cytotoxic activity against HeLa (IC50 = 6.7 ± 1.1 µg/mL) and U343 (IC50 = 6.7 ± 1.2 µg/mL) cells. In addition, in the used experimental protocols, the treatment with manool was significantly more cytotoxic for different tumor cell lines than for the normal cell line V79 (IC50 = 49.3 ± 3.3 µg/mL), and showed high selectivity. These results suggest that manool may be used to treat cancer without affecting normal cells.
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil.
authors.forename: Pollyanna Francielli
authors.initials: PF
authors.lastname: de Oliveira
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil.
authors.forename: Carla Carolina
authors.initials: CC
authors.lastname: Munari
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil.
authors.forename: Heloiza Diniz
authors.initials: HD
authors.lastname: Nicolella
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil.
authors.forename: Rodrigo Cassio Sola
authors.initials: RC
authors.lastname: Veneziani
authors.affiliation: Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil. denisecrispim2001@yahoo.com.
authors.forename: Denise Crispim
authors.initials: DC
authors.lastname: Tavares
keywords: Cancer cells; Cytotoxicity; Manool; Salvia officinalis; Selectivity