title: Complete genomic characterization and genetic diversity of four European genotype porcine reproductive and respiratory syndrome virus isolates from China in 2011.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is an emerging disease that has caused serious economic losses to the swine industry worldwide. In 2011, a nation-wide surveillance program investigated the prevalence of PRRS viruses (PRRSV) in Chinese breeding swine farms, and four European genotype PRRSV (PRRSV-Type 1) were successfully isolated. To explore the genetic diversity of PRRSV-Type 1 in China, these 4 viral strains were subjected to genome sequencing and analysis. The four isolates shared 87.4-90.7 % nucleotide homology with the Lelystad strain (PRRSV-Type 1 stereotype strain). NSP2, ORF3, and ORF4 were the most variable regions and contained discontinuous deletions or insertions when compared to other PRRSV-Type 1 strains. All isolates fell into separate branches of the subtype 1 of PRRSV-Type 1 phylogenetic tree. This analysis of emerging PRRSV-Type 1 strains revealed previously unrecorded genetic diversity. Close attention should be paid to the prevention and control of this evolving virus.
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Hu
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Han
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Gu
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China. Zhaixy2010@sina.cn.
authors.forename: Xinyan
authors.initials: X
authors.lastname: Zhai
authors.affiliation: OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing, 100125, China. tiankg@263.net.|National Research Center for Veterinary Medicine, Cuiwei Road, High-Tech. District, Luoyang, Henan, China. tiankg@263.net.
authors.forename: Kegong
authors.initials: K
authors.lastname: Tian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D019143:Evolution, Molecular; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Chinese swine herds; Genetic variation; PRRSV-Type 1