title: Proliferative effect of plants used for wound healing in Rio Grande do Sul state, Brazil.
abstract: BACKGROUND
  Wounds are normally resolved in a few days, but chronic wounds represent a major burden because of economic and social factors. Thereby, the search for new agents is ongoing and natural products become a great target. Also, Brazil as a consumer of herbal medicines with rich social diversity is promising for ethnopharmacological studies.
  
  
  OBJECTIVE
  The study aims to find the plants popularly used for wound healing purposes in Rio Grande do Sul state, and test the traditional knowledge through an in vitro screening.
  
  
  METHODS
  Ethnobotanical studies from state of Rio Grande do Sul were analyzed to find the most used plants to treat wounds. The selected species were collected, identified and ethanolic and aqueous extracts were prepared. After, proliferative capacity was accessed by MTT assay in a keratinocyte cell line (HaCaT).
  
  
  RESULTS
  The survey comprehended almost all state regions and led to 117 plant species from 85 genera, from which 14 were selected for in vitro testing. Aqueous extracts from Achyrocline satureioides DC Lam., Matricaria recutita L., Melia azedarach L. and Mirabilis jalapa L. demonstrated the ability to stimulate keratinocyte growth up to 120% in concentrations of 25 µg/mL and 50 µg/mL. The ethanolic extract of A. satureioides was able to stimulate keratinocyte and fibroblast proliferation on the lower concentration tested, 1 µg/mL, being the most promising species.
  
  
  CONCLUSIONS
  The traditional knowledge collected from the ethnobotanical studies was accessed by in vitro investigation and extracts from Achyrocline satureioides, Matricaria recutita, Melia azedarach and Mirabilis jalapa can influence positively cell proliferation.
authors.affiliation: Laboratório de Farmacognosia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, Porto Alegre, RS 90610-000, Brazil.
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Alerico
authors.affiliation: Laboratório de Análises Bioquímicas, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, Porto Alegre, RS 90610-000, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Beckenkamp
authors.affiliation: Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Rua Sarmento Leite 245, Porto Alegre, RS 90050-170, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Vignoli-Silva
authors.affiliation: Laboratório de Análises Bioquímicas, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, Porto Alegre, RS 90610-000, Brazil.
authors.forename: Andréia
authors.initials: A
authors.lastname: Buffon
authors.affiliation: Laboratório de Farmacognosia, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, Porto Alegre, RS 90610-000, Brazil. Electronic address: gilsane@farmacia.ufrgs.br.
authors.forename: Gilsane L
authors.initials: GL
authors.lastname: von Poser
mesh_terms: D001938:Brazil; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D014945:Wound Healing
chemical_list: D010936:Plant Extracts
keywords: Achyrocline satureioides; Brazil; Fibroblasts; Keratinocytes; Proliferation; Wound healing