title: A cold-adapted and glucose-stimulated type II α-glucosidase from a deep-sea bacterium Pseudoalteromonas sp. K8.
abstract: OBJECTIVE
  To express and characterize a putative α-glucosidase, Pagl, from Pseudoalteromonas sp. K8 obtained via genome mining approach.
  
  
  RESULTS
  Pagl was expressed and purified to homogeneity, with a molecular mass of 60 kDa. It was optimally active at pH 8.5 and 30 °C, and showed cold-adapted activity. Pagl exhibited specific activity towards substrates with α-1,4-linkage, with the highest specific activity of 19.4 U/mg for maltose, followed by pNPαG and maltodextrins, suggesting that Pagl belongs to the type II α-glucosidase. Interestingly, the activity of Pagl is significantly enhanced (2.7 times) in the presence of 200 mM glucose.
  
  
  CONCLUSIONS
  The unique catalytic properties of Pagl make it an attractive candidate for several industrial applications.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Chemistry & Biochemistry, Florida International University, Miami, FL, 33199, USA.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Fang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D003080:Cold Temperature; D019295:Computational Biology; D057225:Data Mining; D020536:Enzyme Activators / Q000378:metabolism*; D004795:Enzyme Stability; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D031161:Pseudoalteromonas / Q000201:enzymology* / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D020536:Enzyme Activators; D000520:alpha-Glucosidases; D005947:Glucose
keywords: Cold adapted enzyme; Genome missing; Glucose stimulated enzyme; Pseudoaltemonas; α-Glucosidase