title: Cytotoxic effects of β-carboline alkaloids on human gastric cancer SGC-7901 cells.
abstract: To investigate the cytotoxic effects of β-carboline alkaloids on human gastric cancer SGC-7901 cells. Human gastric cancer SGC-790s1 cells were treated with β-carboline alkaloids at the concentration of 0, 10, 20, 30 and 40 μg/ml for 48 hr. Cell viability was measured by Cell Counting Kit-8 assay. Cell apoptosis was detected by Hoechst 33258 staining and DNA fragmentation analysis. The expression of phosphatase and tensin homolog (PTEN) and extracellular signal-regulated kinase (ERK) was examined by quantitative real-time PCR (qRT-PCR) assay and western blot analysis. β-carboline alkaloids inhibited the growth of SGC-7901 cells concentration dependently. β-carboline alkaloids treated SGC-7901 cells displayed apoptotic nuclei as detected using Hoechst 33258 staining. β-carboline alkaloids also induced DNA ladder, indicative of apoptosis in SGC-7901 cells concentration-dependently. Furthermore, β-carboline alkaloids increased PTEN and decreased ERK mRNA expression in SGC-7901 cells in a concentration dependent manner. They also increased PTEN and decreased ERK protein expression. β-carboline alkaloids inhibit the growth and induce apoptosis of SGC-7901 cells. The cytotoxic effects of β-carboline alkaloids might correlate with increased PTEN expression and decreased ERK expression in SGC-7901 cells.
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Abulimiti
authors.initials: A
authors.lastname: Patima
authors.affiliation: The Fourth Department of Internal Medicine, Traditional Chinese Medicine Hospital Changji Hui Autonomous Prefecture, 831100, Xinjiang Province, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Fanye
authors.initials: F
authors.lastname: Zeng
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Wenting
authors.initials: W
authors.lastname: He
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Lingjuan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Yanghua
authors.initials: Y
authors.lastname: Jie
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lei
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Xinmei
authors.initials: X
authors.lastname: Xie
authors.affiliation: Second Department of Oncology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University Urumqi 830000, Xinjiang Province, P.R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhang
keywords: ERK; PTEN; apoptosis; human SGC-7901 cells; β-carboline alkaloids