title: [Expression and characterization of a novel halohydrin dehalogenase from Tistrella mobilis KA081020-065].
abstract: Halohydrin dehalogenase is of great significance for biodegradation of the chlorinated pollutants, and also serves as an important biocatalyst in the synthesis of chiral pharmaceutical intermediates. A putative halohydrin dehalogenase (HheTM) gene from Tistrella mobilis KA081020-065 was cloned and over-expressed in Escherichia coli BL21 (DE3). The recombinant enzyme was purified by Ni-NTA column and characterized. Gel filtration and SDS-PAGE analysis showed that the native form of HheTM was a tetramer. It exhibited the highest activity at 50 degrees C. The nature and pH of the buffer had a great effect on its activity. The enzyme maintained high stability under the alkaline conditions and below 30 degrees C. HheTM catalyzed the transformation of ethyl(S)-4-chloro-3-hydroxybutyrate in the presence of cyanide, to give ethyl (R)-4-cyano-3-hydroxybutyrate, a key intermediate for the synthesis of atorvastatin.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Yao
authors.forename: Lihua
authors.initials: L
authors.lastname: Cheng
authors.forename: Meixian
authors.initials: M
authors.lastname: Xie
authors.forename: Rongrong
authors.initials: R
authors.lastname: Jia
authors.forename: Huijin
authors.initials: H
authors.lastname: Feng
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Qiaqing
authors.initials: Q
authors.lastname: Wu
authors.forename: Dunming
authors.initials: D
authors.lastname: Zhu
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012244:Rhodospirillaceae / Q000201:enzymology* / Q000235:genetics
chemical_list: C511345:4-chloro-3-hydroxybutyric acid; D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D011994:Recombinant Proteins; D006867:Hydrolases; C430848:halohydrin dehalogenase; D020155:3-Hydroxybutyric Acid