title: A new potential secretion pathway for recombinant proteins in Bacillus subtilis.
abstract: BACKGROUND
  Secretion of cytoplasmic expressed proteins into growth media has significant advantages. Due to the lack of an outer membrane, Bacillus subtilis is considered as a desirable 'cell factory' for the secretion of recombinant proteins. However, bottlenecks in the classical pathway for the secretion of recombinant proteins limit its use on a wide scale. In this study, we attempted to use four typical non-classically secreted proteins as signals to export three recombinant model proteins to the culture medium.
  
  
  RESULTS
  All four non-classically secreted proteins can direct the export of the intrinsically disordered nucleoskeletal-like protein (Nsp). Two of them can guide the secretion of alkaline phosphatase (PhoA). One can lead the secretion of the thermostable β-galactosidase BgaB, which cannot be secreted with the aid of typical Sec-dependent signal peptides.
  
  
  CONCLUSIONS
  Our results show that the non-classically secreted proteins lead the recombinant proteins to the culture medium, and thus non-classical protein secretion pathways can be exploited as a novel secretion pathway for recombinant proteins.
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China. 1015wanggq@163.com.|State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. 1015wanggq@163.com.
authors.forename: Guangqiang
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China. dreamup@126.com.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. zhennangu@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, Jiangsu, People's Republic of China. zhennangu@jiangnan.edu.cn.
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. zhanghao@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, Jiangsu, People's Republic of China. zhanghao@jiangnan.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. yqchen@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, Jiangsu, People's Republic of China. yqchen@jiangnan.edu.cn.
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. haiqinchen@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, Jiangsu, People's Republic of China. haiqinchen@jiangnan.edu.cn.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China. ailianzhong@163.com.
authors.forename: Lianzhong
authors.initials: L
authors.lastname: Ai
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China. chenwei66@jiangnan.edu.cn.|Synergetic Innovation Center of Food Safety and Nutrition, Wuxi, 214122, Jiangsu, People's Republic of China. chenwei66@jiangnan.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005838:Genotype; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase