title: In vitro evidence for efficacy in food intolerance for the multispecies probiotic formulation Ecologic® Tolerance (Syngut™).
abstract: The beneficial effects of probiotics are currently the subject of extensive studies in health and medical research. The aim of this research was to specifically design a new probiotic formulation for supplementation in people suffering from food intolerance. The selection of strains was focussed on the capacity to influence mechanisms of action that are important in development of food intolerance with the following parameters measure: in vitro capacity to produce β-galactosidase, in vitro strengthening of the epithelial barrier, in vitro stimulation of cytokines produced by regulatory T cells, in addition to assessing fundamental quality criteria (stability, gastrointestinal (GI)-survival, multispecies concept, allergen-free). Ecologic®Tolerance/Syngut™ was subsequently developed consisting of a multispecies concept using 4 different probiotic strains (Bifidobacterium lactis W51, Lactobacillus acidophilus W22, Lactobacillus plantarum W21 and Lactococcus lactis W19). Each of these strains demonstrated ability to survive the GI-tract and strain specific effects in producing β-galactosidase, strengthening the gut barrier function after immunological-induced stress and inhibiting Th2 cytokines (IL-4, IL-5 and IL-13 (≥50%), in addition to stimulating interleukin-10 levels; thus, providing in vitro evidence for the efficacy of the selected strains to provide beneficial effects in patients suffering from food intolerance.
authors.affiliation: 1 Winclove Probiotics B.V., Hulstweg 11, 1032 LB Amsterdam, the Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Besseling-van der Vaart
authors.affiliation: 2 Allergy Therapeutics plc., Dominion Way, Worthing, West Sussex BN14 8SA, United Kingdom.
authors.forename: M D
authors.initials: MD
authors.lastname: Heath
authors.affiliation: 3 Allergy Therapeutics Italia, Via IV Novembre 76, 20019 Settimo Milanese, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Guagnini
authors.affiliation: 4 Bencard Allergy GmbH, Messerschmitstrasse 4, 80992 München, Germany.
authors.forename: M F
authors.initials: MF
authors.lastname: Kramer
mesh_terms: D001644:Bifidobacterium; D018938:Caco-2 Cells; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D005512:Food Hypersensitivity / Q000178:diet therapy / Q000517:prevention & control*; D006801:Humans; D007444:Inulin / Q000494:pharmacology; D007779:Lactobacillus acidophilus; D048191:Lactiplantibacillus plantarum; D013294:Lactococcus lactis; D008903:Minerals / Q000494:pharmacology; D019936:Probiotics / Q000737:chemistry; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D016207:Cytokines; D008903:Minerals; D007444:Inulin; D001616:beta-Galactosidase
keywords: food intolerance; gut barrier function; immune modulation; probiotics; synbiotics