title: [Methylglyoxal inhibits human umbilical vein cell migration in vitro by down-regulating integrinβ3].
abstract: OBJECTIVE
  To explore the effects of methylglyoxal on endothelia cell migration.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) were stimulated by serial concentrations of methylglyoxal (MGO, 0, 25, 50, 100 and 200 µmol/L) for 24 h, and the cell migration was assessed by scratch wound and Transwell assay. The expression of integrin β3 in the treated cells was examined by immunoblotting, and the effect of an anti-β3 antibody, LM609, on cell migration was investigated.
  
  
  RESULTS
  Methylglyoxal significantly inhibited HUVEC migration in a concentration-dependent manner (P<0.05). Methylglyoxal decreased the expression of integrin β3 in a time- and concentration-dependent manner (P<0.05). LM609 also significantly inhibited HUVEC migration (P<0.05).
  
  
  CONCLUSIONS
  Methylglyoxal inhibits HUVEC migration in vitro by down-regulating integrin β3 expression.
authors.affiliation: Drug Discovery Research Center, Sichuan Medical University, Luzhou 646000, China. E-mail: 1046392469@qq.com.
authors.forename: Ning-Bo
authors.initials: NB
authors.lastname: Pang
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Wang
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wu
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D015536:Down-Regulation; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000494:pharmacology*
chemical_list: D039661:Integrin beta3; D011765:Pyruvaldehyde