title: Application of NMR Spectroscopy in the Assessment of Radiation Dose in Human Primary Cells.
abstract: We employed the primary cell model system as a first step toward establishing a method to assess the influence of ionizing radiation by using a combination of common and abundant metabolites. We applied X-ray irradiation amounts of 0, 1, and 5 Gy to the cells that were harvested 24, 48, or 72 h later, and profiled metabolites by 2D-NMR spectroscopy to sort out candidate molecules that could be used to distinguish the samples under different irradiation conditions. We traced metabolites stemming from the input ¹³C-glucose, identified twelve of them from the cell extracts, and applied statistical analysis to find out that all the metabolites, including glycine, alanine, and gluatamic acid, increased upon irradiation. The combinatorial use of the selected metabolites showed promising results where the product of signal intensities of alanine and lactate could differentiate samples according to the dose of X-ray irradiation. We hope that this work can form a base for treating radiation-poisoned patients in the future.
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: Chang-Mo
authors.initials: CM
authors.lastname: Kang
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.|Department of Chemistry, Sejong University, Seoul 143 - 747, Republic of Korea, (phone: +82-2-3408-3748; fax: +82-2-3408-4317).
authors.forename: Jin
authors.initials: J
authors.lastname: Seong Hyeon
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: So
authors.initials: S
authors.lastname: Ra Kim
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: Eun
authors.initials: E
authors.lastname: Kyeong Lee
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Jin Yun
authors.affiliation: Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 139-706, Republic of Korea.
authors.forename: Sun
authors.initials: S
authors.lastname: Young Kim
authors.affiliation: Department of Chemistry, Sejong University, Seoul 143 - 747, Republic of Korea, (phone: +82-2-3408-3748; fax: +82-2-3408-4317).
authors.forename: Young
authors.initials: Y
authors.lastname: Kee Chae
mesh_terms: D000409:Alanine / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D061251:Primary Cell Culture; D014965:X-Rays
chemical_list: D018698:Glutamic Acid; D000409:Alanine; D005998:Glycine
keywords: Metabolite profiling; NMR Spectroscopy; Primary cell; X-Ray irradiation