title: Ultrasensitive non-enzymatic immunosensor for carcino-embryonic antigen based on palladium hybrid vanadium pentoxide/multiwalled carbon nanotubes.
abstract: A novel and sensitive sandwich-type non-enzymatic electrochemical immunosensor was fabricated for quantitative monitoring of carcino-embryonic antigen (CEA). Nanocomposite of stannic oxide/reduced graphene oxide was used as substrate material to increase the specific surface area and enhance the conductivity of the glassy carbon electrode. Gold nanoparticles (Au NPs) were introduced to link substrate materials and primary antibodies (Ab1) and accelerate the electron transfer in this system. At the same time, the palladium nanoparticles (Pd NPs)-vanadium pentoxide (V2O5)/multiwalled carbon nanotubes (MWCNTs) were used as the label of secondary antibodies (Ab2). This composite label has shown excellent catalytic activity towards the reduction of H2O2. The nanomaterial-based signal amplification can improve the sensitivity and lower the limit of detection. The proposed immunosensor showed wide linear range from 0.5 pgmL(-1) to 25 ngmL(-1) with limit of detection of 0.17 pgmL(-1). This novel immunosensor was used to analyze serum sample. The results indicated that this immunosensor may find huge potential application for quantitative detection of CEA in the clinical diagnosis.
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Faying
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Dong
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China. Electronic address: liyueyun71@163.com.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000097:blood* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017968:Vanadium Compounds / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D037742:Nanotubes, Carbon; D017968:Vanadium Compounds; D010165:Palladium; C066075:vanadium pentoxide
keywords: Gold nanoparticles; Multi-walled carbon nanotubes; Palladium nanoparticles; Stannic oxide/reduced graphene oxide nanocomposite; Vanadium pentoxide