title: EFFECT OF SOLID MEDIUM DURING COOLED STORAGE ON STALLION SPERM PARAMETERS.
abstract: BACKGROUND
  Solid storage medium prevents cellular sedimentation, reduces metabolic demand via limiting movement, and avoids the modification of an extender composition in the sedimentary microenvironment. It has been proven to prolong spermatozoa viability in mammalians.
  
  
  OBJECTIVE
  This experiment aims to evaluate the effect of cool storage in solid phase extender on stallion sperms.
  
  
  METHODS
  Semen was collected from 10 Crioulo stallions (n=30) and submitted to treatments: control group (semen extender) and groups with gelatin addition in different concentrations (semen extender + 1%, 2% and 3%). Seminal analyses included motility, mitochondrial functionality, plasma membrane integrity, DNA and acrosome at 0; 24; 48 and 72 hours during cooled storage at 5 degree C.
  
  
  RESULTS
  Motility, mitochondrial functionality, plasma membrane and acrosome integrity declined during storage time, with no statistical difference between treatments. DNA integrity did not significantly change during storage period.
  
  
  CONCLUSIONS
  Solid medium was not harmful and did not improved stallion sperm parameters during cooled storage.
authors.affiliation: Departamento de Clinicas Veterinarias, Faculdade de Veterinaria, Universidade; Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil. antoniovarela@furg.br and carlini@portoweb.com.br.
authors.forename: F C
authors.initials: FC
authors.lastname: Santos
authors.affiliation: Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil.
authors.forename: C D
authors.initials: CD
authors.lastname: Corcini
authors.affiliation: Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil.
authors.forename: V G
authors.initials: VG
authors.lastname: Costa
authors.affiliation: Departamento de Patologia Animal, Laboratorio de Reproduçao Animal, Federal de Pelotas, Campus Universitario, Pelotas, Brasil.
authors.forename: S M
authors.initials: SM
authors.lastname: Gheller
authors.affiliation: Departamento de Clinicas Veterinarias, Faculdade de Veterinaria, Universidade, Federal de Pelotas, Campus Universitario, Pelotas, Brasil.
authors.forename: C E
authors.initials: CE
authors.lastname: Nogueira
authors.affiliation: Departamento de Clinicas Veterinarias, Faculdade de Veterinaria, Universidade, Federal de Pelotas, Campus Universitario, Pelotas, Brasil.
authors.forename: B
authors.initials: B
authors.lastname: da Rosa Curcio
authors.affiliation: Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Faculdade de Veterinaria, Universidade, Federal de Pelotas; Reproduçao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Varel
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D006736:Horses / Q000502:physiology*; D008297:Male; D012661:Semen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D003451:Cryoprotective Agents; D005780:Gelatin