title: MzPIP2;1: An Aquaporin Involved in Radial Water Movement in Both Water Uptake and Transportation, Altered the Drought and Salt Tolerance of Transgenic Arabidopsis.
abstract: BACKGROUND
  Plants are unavoidably subjected to various abiotic stressors, including high salinity, drought and low temperature, which results in water deficit and even death. Water uptake and transportation play a critical role in response to these stresses. Many aquaporin proteins, localized at different tissues, function in various transmembrane water movements. We targeted at the key aquaporin in charge of both water uptake in roots and radial water transportation from vascular tissues through the whole plant.
  
  
  RESULTS
  The MzPIP2;1 gene encoding a plasma membrane intrinsic protein was cloned from salt-tolerant apple rootstock Malus zumi Mats. The GUS gene was driven by MzPIP2;1 promoter in transgenic Arabidopsis. It indicated that MzPIP2;1 might function in the epidermal and vascular cells of roots, parenchyma cells around vessels through the stems and vascular tissues of leaves. The ectopically expressed MzPIP2;1 conferred the transgenic Arabidopsis plants enhanced tolerance to slight salt and drought stresses, but sensitive to moderate salt stress, which was indicated by root length, lateral root number, fresh weight and K+/Na+ ratio. In addition, the possible key cis-elements in response to salt, drought and cold stresses were isolated by the promoter deletion experiment.
  
  
  CONCLUSIONS
  The MzPIP2;1 protein, as a PIP2 aquaporins subgroup member, involved in radial water movement, controls water absorption and usage efficiency and alters transgenic plants drought and salt tolerance.
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Qingtian
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Lei
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Biological Sciences, China Agricultural University, Beijing, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Horticultural Plants, China Agricultural University, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Kong
mesh_terms: D000595:Amino Acid Sequence; D020346:Aquaporins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D027845:Malus / Q000235:genetics* / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011188:Potassium / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014867:Water / Q000378:metabolism*
chemical_list: D020346:Aquaporins; D010940:Plant Proteins; D020033:Protein Isoforms; D014867:Water; D012965:Sodium Chloride; D012964:Sodium; D005966:Glucuronidase; D011188:Potassium