title: Identification of Novel Inhibitors for Tobacco Mosaic Virus Infection in Solanaceae Plants.
abstract: Tobacco mosaic virus (TMV) infects several crops of economic importance (e.g., tomato) and remains as one of the major concerns to the farmers. TMV enters the host cell and produces the capping enzyme RNA polymerase. The viral genome replicates further to produce multiple mRNAs which encodes several proteins, including the coat protein and an RNA-dependent RNA polymerase (RdRp), as well as the movement protein. TMV replicase domain was chosen for the virtual screening studies against small molecules derived from ligand databases such as PubChem and ChemBank. Catalytic sites of the RdRp domain were identified and subjected to docking analysis with screened ligands derived from virtual screening LigandFit. Small molecules that interact with the target molecule at the catalytic domain region amino acids, GDD, were chosen as the best inhibitors for controlling the TMV replicase activity.
authors.affiliation: Data Mining & Text Mining Laboratory, Department of Bioinformatics, School of Life Sciences, Bharathiar University, Coimbatore, Tamilnadu 641046, India ; Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu 641003, India.
authors.forename: Archana
authors.initials: A
authors.lastname: Prabahar
authors.affiliation: Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu 641003, India.
authors.forename: Subashini
authors.initials: S
authors.lastname: Swaminathan
authors.affiliation: Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu 641003, India.
authors.forename: Arul
authors.initials: A
authors.lastname: Loganathan
authors.affiliation: Department of Plant Molecular Biology & Bioinformatics, Centre for Plant Molecular Biology & Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu 641003, India.
authors.forename: Ramalingam
authors.initials: R
authors.lastname: Jegadeesan