title: Anti-inflammatory properties of Morus bombycis Koidzumi via inhibiting IFN-β signaling and NLRP3 inflammasome activation.
abstract: BACKGROUND
  Morus bombycis Koidzumi (M. bombycis, Moraceae) has been used in Asian countries as a traditional medicine for the treatment of hypertension, diabetes, and inflammation-related disorders.
  
  
  OBJECTIVE
  Although its anti-inflammatory actions have been partly documented, scientific evidence involving its molecular mechanisms related to inflammasome activation signaling pathways remains unknown.
  
  
  METHODS
  Lipopolysaccharide-stimulated RAW 264.7 cells and bone marrow-derived murine macrophages were used to study the in vitro effect of methanolic extract of M. bombycis (MB) on inflammatory responses. A monosodium urate crystal (MSU)-induced peritonitis murine model was used to study the in vivo effects.
  
  
  RESULTS
  MB attenuated the production of nitric oxide and interleukin-6, through the regulation of the interferon-β receptor signaling pathway. MB also inhibited IL-1β secretion via attenuation of NLRP3 inflammasome activation. Furthermore, MB inhibited MSU-induced peritonitis in the in vivo murine model.
  
  
  CONCLUSIONS
  This study provides the key molecular mechanisms involved in the anti-inflammatory effects of M. bombycis, substantiating the traditional claims of its use in the treatment of inflammation-related disorders.
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Na-Hyun
authors.initials: NH
authors.lastname: Oh
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Han
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Do-Wan
authors.initials: DW
authors.lastname: Shim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Sim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Sushruta
authors.initials: S
authors.lastname: Koppula
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Su-Bin
authors.initials: SB
authors.lastname: Kwak
authors.affiliation: Department of Food Science and Engineering, Seowon University, Cheongju, South Korea.
authors.forename: Myong-Ki
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Young-Kyu
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Yanbian University, Yanji, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea. Electronic address: kangtbko@gmail.com.
authors.forename: Tae-Bong
authors.initials: TB
authors.lastname: Kang
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju, South Korea. Electronic address: kwangho@kku.ac.kr.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D058847:Inflammasomes; D016899:Interferon-beta / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008810:Mice, Inbred C57BL; D031625:Morus; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009569:Nitric Oxide / Q000378:metabolism; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D017382:Reactive Oxygen Species / Q000378:metabolism; D014527:Uric Acid
chemical_list: D000893:Anti-Inflammatory Agents; D002352:Carrier Proteins; D058847:Inflammasomes; D015850:Interleukin-6; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C577686:interleukin-6, mouse; D014527:Uric Acid; D009569:Nitric Oxide; D016899:Interferon-beta
keywords: Anti-inflammation; Inflammasome; Morus bombycis; Peritonitis; TRIF