title: Analysis of iminosugars and other low molecular weight carbohydrates in Aglaonema sp. extracts by hydrophilic interaction liquid chromatography coupled to mass spectrometry.
abstract: A method by hydrophilic interaction liquid chromatography coupled to tandem mass spectrometry (HILIC-MS(2)) has been successfully developed for the simultaneous analysis of bioactive iminosugars and other low molecular weight carbohydrates in Aglaonema leaf extracts. Among other experimental chromatographic conditions, mobile phase eluents, additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry provided for target carbohydrates. In general, narrow peaks (wh: 0.2-0.6min) with good symmetry (As: 0.9-1.3) and excellent resolution (Rs>1.8) were obtained for iminosugars using an acetonitrile:water gradient with 5mM ammonium acetate in both eluents at 55°C. Tandem mass spectra were used to confirm the presence of previously detected iminosugars in Aglaonema extracts and to tentatively identify for the first time others such as miglitol isomer, glycosyl-miglitol isomers and glycosyl-DMDP isomers. Concentration of total iminosugars varied from 1.35 to 2.84mgg(-1) in the extracts of the different Aglaonema samples analyzed. To the best of our knowledge, this is the first time that a HILIC-MS(2) method has been proposed for the simultaneous analysis of iminosugars and other low molecular weight carbohydrates of Aglaonema sp. extracts.
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Rodríguez-Sánchez
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: García-Sarrió
authors.affiliation: Instituto de Química-Física Rocasolano (CSIC), Serrano 119, 28006 Madrid, Spain.
authors.forename: J E
authors.initials: JE
authors.lastname: Quintanilla-López
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
authors.forename: A C
authors.initials: AC
authors.lastname: Soria
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. Electronic address: mlsanz@iqog.csic.es.
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D029064:Araceae / Q000737:chemistry*; D002241:Carbohydrates / Q000032:analysis*; D002853:Chromatography, Liquid; D057927:Hydrophobic and Hydrophilic Interactions; D050111:Imino Sugars / Q000032:analysis; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D002241:Carbohydrates; D050111:Imino Sugars; D010936:Plant Extracts; D014867:Water; C032159:acetonitrile
keywords: Aglaonema extracts; Hydrophilic interaction liquid chromatography; Iminosugars; Low molecular weight carbohydrates; Tandem mass spectrometry