title: Tachykinin acts upstream of autocrine Hedgehog signaling during nociceptive sensitization in Drosophila.
abstract: Pain signaling in vertebrates is modulated by neuropeptides like Substance P (SP). To determine whether such modulation is conserved and potentially uncover novel interactions between nociceptive signaling pathways we examined SP/Tachykinin signaling in a Drosophila model of tissue damage-induced nociceptive hypersensitivity. Tissue-specific knockdowns and genetic mutant analyses revealed that both Tachykinin and Tachykinin-like receptor (DTKR99D) are required for damage-induced thermal nociceptive sensitization. Electrophysiological recording showed that DTKR99D is required in nociceptive sensory neurons for temperature-dependent increases in firing frequency upon tissue damage. DTKR overexpression caused both behavioral and electrophysiological thermal nociceptive hypersensitivity. Hedgehog, another key regulator of nociceptive sensitization, was produced by nociceptive sensory neurons following tissue damage. Surprisingly, genetic epistasis analysis revealed that DTKR function was upstream of Hedgehog-dependent sensitization in nociceptive sensory neurons. Our results highlight a conserved role for Tachykinin signaling in regulating nociception and the power of Drosophila for genetic dissection of nociception.
authors.affiliation: Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, United States.
authors.forename: Seol Hee
authors.initials: SH
authors.lastname: Im
authors.affiliation: Department of Neurobiology, University of Massachusetts Medical School, Worcester, United States.
authors.forename: Kendra
authors.initials: K
authors.lastname: Takle
authors.affiliation: Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, United States.|Genes and Development Graduate Program, University of Texas Graduate School of Biomedical Sciences, Houston, United States.
authors.forename: Juyeon
authors.initials: J
authors.lastname: Jo
authors.affiliation: Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, United States.|Neuroscience Graduate Program, University of Texas Graduate School of Biomedical Sciences, Houston, United States.
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Babcock
authors.affiliation: Department of Neurobiology, University of Massachusetts Medical School, Worcester, United States.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Neurobiology, University of Massachusetts Medical School, Worcester, United States.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, United States.|Genes and Development Graduate Program, University of Texas Graduate School of Biomedical Sciences, Houston, United States.|Neuroscience Graduate Program, University of Texas Graduate School of Biomedical Sciences, Houston, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Galko
mesh_terms: D000200:Action Potentials; D000818:Animals; D004330:Drosophila / Q000502:physiology* / Q000528:radiation effects; D029721:Drosophila Proteins / Q000378:metabolism; D055724:Electrophysiological Phenomena; D053823:Hedgehog Proteins / Q000378:metabolism*; D006358:Hot Temperature; D009619:Nociceptors / Q000502:physiology*; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D015398:Signal Transduction; D015320:Tachykinins / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D017981:Receptors, Neurotransmitter; D015320:Tachykinins; C071259:TkR99D protein, Drosophila
keywords: D. melanogaster; Hedgehog; Substance P; Trp channels; electrophysiology; neuropeptides; neuroscience; nociceptive sensitization