title: [Optimization of method for determination of Salviae Miltiorrhizae Radix et Rhizoma].
abstract: OBJECTIVE
  To optimize the method in the Chinese Pharmacopoeia for determining Salviae Miltiorrhizae Radix et Rhizoma.
  
  
  METHODS
  Tanshinone II(A) and salvianolic acid B were selected as the index in optimization of the sample preparation method of Salviae Miltiorrhizae Radix et Rhizoma in Chinese Pharmacopoeia. Orthogonal test was used to optimize the extraction process of Salviae Miltiorrhizae Radix et Rhizoma, and concentration of contents were detected by high performance liquid chromatography method. A detection of using methanol-water (85: 15) at wavelength of 270 nm was employed for tanshinone II(A) and a detection of using methanol-acetonitrile-formic acid-water (30:10:1: 59) at wavelength of 286 nm was employed for salvianolic acid B.
  
  
  RESULTS
  The optimized extraction process of tanshinone II(A) and salvianolic acid B was: extracted by 90% methanol and reflux twice (0.5 h each time) at 75 degrees C, extracted by 70% methanol and reflux twice (1.5 h each time) at 75 degrees C, respectively.
  
  
  CONCLUSIONS
  Optimized extraction and determination methods could be used to reflect the content of tanshinone II(A) and salvianolic acid B in Salviae Miltiorrhizae Radix et Rhizoma more accurately and efficiently.
authors.forename: Nian-jun
authors.initials: NJ
authors.lastname: Yu
authors.forename: Dai-yin
authors.initials: DY
authors.lastname: Peng
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Chen
authors.forename: Hua-sheng
authors.initials: HS
authors.lastname: Peng
authors.forename: Fan
authors.initials: F
authors.lastname: Yu
authors.forename: Shun-min
authors.initials: SM
authors.lastname: Shi
mesh_terms: D045784:Abietanes / Q000032:analysis; D001572:Benzofurans / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D027343:Rhizome / Q000737:chemistry; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D013696:Temperature; D053630:Depsides
chemical_list: D045784:Abietanes; D001572:Benzofurans; C021751:tanshinone; C076944:salvianolic acid B; D053630:Depsides