title: [Phage display peptide library for screening the peptides that specifically bind to osteoblasts cells].
abstract: OBJECTIVE
  To explore the experimental methods that the phage peptide library technology screening human osteoblast specificity polypeptide, which will provide the basis of the experiment of the Ti surface biolization modification.
  
  
  METHODS
  Human calvarial osteoblasts were used as the target cells for whole-cell biopanning from a 12-mer peptide phage-display library. Cell eluent and cell lysis buffer were cultivate and count respectively after washing. Then the target cells were analyzed by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection to authenticate the positive phage clones by human gingival fibroblast as the absorber cells. The positive phage clones were deduced by DNA sequencing.
  
  
  RESULTS
  After four rounds of screening, twenty-two positive phage clones were found out from randomly selected phage monoclonals, whose single-strand DNA were extracted and sequenced. Amino acid sequence of the highest frequency peptide was MGWSWWPETWPM.
  
  
  CONCLUSIONS
  The specific peptide against human osteoblasts can be obtained from a phage-display peptide library for use as a new research approach and experimental basis of the biolization modification of the titanium surface.
authors.affiliation: Outpatient Department of Government District, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China; Email: wumingyue321@126.com.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Wu
authors.affiliation: Outpatient Department of Government District, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Outpatient Department of Government District, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Du
authors.affiliation: Outpatient Department of Government District, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qian
authors.affiliation: Outpatient Department of Government District, Stomatologic Hospital and College, Anhui Medical University & Key Laboratory of Oral Diseases Research of Anhui Province, Hefei 230032, China.
authors.forename: Quanli
authors.initials: Q
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D010006:Osteoblasts; D019151:Peptide Library; D010455:Peptides / Q000235:genetics*; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014025:Titanium
chemical_list: D019151:Peptide Library; D010455:Peptides; D014025:Titanium