title: The phenome analysis of mutant alleles in Leucine-Rich Repeat Receptor-Like Kinase genes in rice reveals new potential targets for stress tolerant cereals.
abstract: Plants are constantly exposed to a variety of biotic and abiotic stresses that reduce their fitness and performance. At the molecular level, the perception of extracellular stimuli and the subsequent activation of defense responses require a complex interplay of signaling cascades, in which protein phosphorylation plays a central role. Several studies have shown that some members of the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) family are involved in stress and developmental pathways. We report here a systematic analysis of the role of the members of this gene family by mutant phenotyping in the monocotyledon model plant rice, Oryza sativa. We have then targeted 176 of the ∼320 LRR-RLK genes (55.7%) and genotyped 288 mutant lines. Position of the insertion was confirmed in 128 lines corresponding to 100 LRR-RLK genes (31.6% of the entire family). All mutant lines harboring homozygous insertions have been screened for phenotypes under normal conditions and under various abiotic stresses. Mutant plants have been observed at several stages of growth, from seedlings in Petri dishes to flowering and grain filling under greenhouse conditions. Our results show that 37 of the LRR-RLK rice genes are potential targets for improvement especially in the generation of abiotic stress tolerant cereals.
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France. Electronic address: anne.dievart@cirad.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Dievart
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Perin
authors.affiliation: INRA, UMR BGPI, INRA-CIRAD-SupAgro, TA 54/K, Campus International de Baillarguet, 34398 Montpellier cedex 5, France.
authors.forename: Judith
authors.initials: J
authors.lastname: Hirsch
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Bettembourg
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Lanau
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Artus
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Bureau
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Noel
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Gaétan
authors.initials: G
authors.lastname: Droc
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Peyramard
authors.affiliation: INRA, UMR BGPI, INRA-CIRAD-SupAgro, TA 54/K, Campus International de Baillarguet, 34398 Montpellier cedex 5, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Pereira
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Courtois
authors.affiliation: INRA, UMR BGPI, INRA-CIRAD-SupAgro, TA 54/K, Campus International de Baillarguet, 34398 Montpellier cedex 5, France.
authors.forename: Jean-Benoit
authors.initials: JB
authors.lastname: Morel
authors.affiliation: CIRAD, UMR AGAP, 34398 Montpellier cedex 5, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Guiderdoni
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000483:Alleles; D016000:Cluster Analysis; D018548:Cotyledon / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D002523:Edible Grain / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D005838:Genotype; D008353:Mannitol / Q000494:pharmacology; D005810:Multigene Family; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000145:classification / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride; D017346:Protein Serine-Threonine Kinases
keywords: Abiotic stress; LRR RLK; Mutant; Rice