title: Low-dose exposure of silica nanoparticles induces cardiac dysfunction via neutrophil-mediated inflammation and cardiac contraction in zebrafish embryos.
abstract: The toxicity mechanism of nanoparticles on vertebrate cardiovascular system is still unclear, especially on the low-level exposure. This study was to explore the toxic effect and mechanisms of low-dose exposure of silica nanoparticles (SiNPs) on cardiac function in zebrafish embryos via the intravenous microinjection. The dosage of SiNPs was based on the no observed adverse effect level (NOAEL) of malformation assessment in zebrafish embryos. The mainly cardiac toxicity phenotypes induced by SiNPs were pericardial edema and bradycardia but had no effect on atrioventricular block. Using o-Dianisidine for erythrocyte staining, the cardiac output of zebrafish embryos was decreased in a dose-dependent manner. Microarray analysis and bioinformatics analysis were performed to screen the differential expression genes and possible pathway involved in cardiac function. SiNPs induced whole-embryo oxidative stress and neutrophil-mediated cardiac inflammation in Tg(mpo:GFP) zebrafish. Inflammatory cells were observed in atrium of SiNPs-treated zebrafish heart by histopathological examination. In addition, the expression of TNNT2 protein, a cardiac contraction marker in heart tissue had been down-regulated compared to control group using immunohistochemistry. Confirmed by qRT-PCR and western blot assays, results showed that SiNPs inhibited the calcium signaling pathway and cardiac muscle contraction via the down-regulated of related genes, such as ATPase-related genes (atp2a1l, atp1b2b, atp1a3b), calcium channel-related genes (cacna1ab, cacna1da) and the regulatory gene tnnc1a for cardiac troponin C. Moreover, the protein level of TNNT2 was decreased in a dose-dependent manner. For the first time, our results demonstrated that SiNPs induced cardiac dysfunction via the neutrophil-mediated cardiac inflammation and cardiac contraction in zebrafish embryos.
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Junchao
authors.initials: J
authors.lastname: Duan
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Li
authors.affiliation: c Hunter Biotechnology Inc. , Hangzhou, Zhejiang Province , P.R. China.
authors.forename: Hongcui
authors.initials: H
authors.lastname: Liu
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Li
authors.initials: L
authors.lastname: Jing
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Man
authors.initials: M
authors.lastname: Yang
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.affiliation: c Hunter Biotechnology Inc. , Hangzhou, Zhejiang Province , P.R. China.
authors.forename: Chunqi
authors.initials: C
authors.lastname: Li
authors.affiliation: a School of Public Health, Capital Medical University , Beijing , P.R. China .|b Beijing Key Laboratory of Environmental Toxicology, Capital Medical University , Beijing , P.R. China , and.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000276:immunology; D006321:Heart / Q000187:drug effects* / Q000196:embryology; D006334:Heart Function Tests; D007249:Inflammation / Q000139:chemically induced; D009200:Myocardial Contraction / Q000187:drug effects*; D053758:Nanoparticles / Q000633:toxicity*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D012822:Silicon Dioxide / Q000633:toxicity*; D015027:Zebrafish / Q000097:blood / Q000196:embryology / Q000276:immunology*
chemical_list: D012822:Silicon Dioxide
keywords: Cardiac dysfunction; cardiac muscle contraction; inflammation; silica nanoparticles; zebrafish