title: F1 -ATP synthase α-subunit: a potential target for RNAi-mediated pest management of Locusta migratoria manilensis.
abstract: BACKGROUND
  The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase (F0 F1 -ATPase) uses proton or sodium motive force to produce 90% of the cellular ATP, and the α-subunit of F1 -ATP synthase (ATP5A) is vital for F1 -ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi-mediated pest management of L. migratoria.
  
  
  RESULTS
  Lm-ATP5A was cloned and characterised. Lm-ATP5A is expressed in all tissues. Injection of 100 ng of the double-stranded RNA of ATP5A (dsATP5A) knocked down the transcription of the target gene and caused mortality in 1.5-5 days. The Lm-ATP5A protein level, the oligomycin-sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection.
  
  
  CONCLUSIONS
  These findings demonstrated the essential roles of Lm-ATP5A in L. migratoria and identified it as a potential target for insect pest control. © 2015 Society of Chemical Industry.
authors.affiliation: Genetic Engineering Research Centre, College of Life Science, Chongqing University, Chongqing, China.|Chongqing Engineering Research Centre for Fungal Insecticide, Chongqing, China.|Key Laboratory of Gene Function and Regulation Technologies under the Chongqing Municipal Education Commission, Chongqing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Genetic Engineering Research Centre, College of Life Science, Chongqing University, Chongqing, China.|Chongqing Engineering Research Centre for Fungal Insecticide, Chongqing, China.|Key Laboratory of Gene Function and Regulation Technologies under the Chongqing Municipal Education Commission, Chongqing, China.
authors.forename: Yuxian
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D055785:Gene Knockdown Techniques / Q000379:methods; D007300:Insect Control / Q000379:methods*; D048188:Locusta migratoria / Q000201:enzymology*; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000187:drug effects* / Q000235:genetics; D034622:RNA Interference
chemical_list: D006180:Proton-Translocating ATPases
keywords: ATP synthase α-subunit; RNA interference (RNAi); pest control; potential target