title: Dietary Fiber-Induced Improvement in Glucose Metabolism Is Associated with Increased Abundance of Prevotella.
abstract: The gut microbiota plays an important role in human health by interacting with host diet, but there is substantial inter-individual variation in the response to diet. Here we compared the gut microbiota composition of healthy subjects who exhibited improved glucose metabolism following 3-day consumption of barley kernel-based bread (BKB) with those who responded least to this dietary intervention. The Prevotella/Bacteroides ratio was higher in responders than non-responders after BKB. Metagenomic analysis showed that the gut microbiota of responders was enriched in Prevotella copri and had increased potential to ferment complex polysaccharides after BKB. Finally, germ-free mice transplanted with microbiota from responder human donors exhibited improved glucose metabolism and increased abundance of Prevotella and liver glycogen content compared with germ-free mice that received non-responder microbiota. Our findings indicate that Prevotella plays a role in the BKB-induced improvement in glucose metabolism observed in certain individuals, potentially by promoting increased glycogen storage.
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Petia
authors.initials: P
authors.lastname: Kovatcheva-Datchary
authors.affiliation: Food for Health Science Centre, Lund University, Medicon Village, Lund 22381, Sweden.
authors.forename: Anne
authors.initials: A
authors.lastname: Nilsson
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Rozita
authors.initials: R
authors.lastname: Akrami
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Ying Shiuan
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Filipe
authors.initials: F
authors.lastname: De Vadder
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Tulika
authors.initials: T
authors.lastname: Arora
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Hallen
authors.affiliation: Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109-5620, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Martens
authors.affiliation: Food for Health Science Centre, Lund University, Medicon Village, Lund 22381, Sweden.
authors.forename: Inger
authors.initials: I
authors.lastname: Björck
authors.affiliation: Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg 41345, Sweden; Novo Nordisk Foundation Center for Basic Metabolic Research, Section for Metabolic Receptology and Enteroendocrinology, Faculty of Health Sciences, University of Copenhagen, Copenhagen 2200, Denmark. Electronic address: fredrik.backhed@wlab.gu.se.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Bäckhed
mesh_terms: D000368:Aged; D000818:Animals; D001439:Bacteroides / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D001786:Blood Glucose / Q000032:analysis; D018592:Cross-Over Studies; D004043:Dietary Fiber / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D006859:Hydrogen / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007422:Intestines / Q000382:microbiology; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000187:drug effects*; D008875:Middle Aged; D018720:Prevotella / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004043:Dietary Fiber; D007328:Insulin; D012336:RNA, Ribosomal, 16S; D006859:Hydrogen; D006003:Glycogen; D005947:Glucose