title: Determination of residual arsenic compounds in chicken muscle by ultra-performance liquid chromatography coupled with ultraviolet detection after pre-column derivatization with toluene-3,4-dithiol.
abstract: A simple and sensitive derivatization method using toluene-3,4-dithiol as a derivatization reagent for the simultaneous analysis of seven arsenic compounds (roxarsone, nitarsone, p-arsanilic acid, o-arsanilic acid, phenylarsonic acid, phenylarsine oxide, and mono-methylarsonic acid) in chicken muscle was developed and validated by ultra-performance liquid chromatography coupled with ultraviolet detection (UPLC-UV). The structure of the derivatized arsenic compounds was confirmed by liquid chromatography-ion trap mass spectrometry or gas chromatography-mass spectrometry. Optimization of the derivatization reaction conditions was carried out by investigating the influence of reagent concentration, buffer or additive acids, temperature, and time. The optimized conditions were a derivatization reagent concentration of 20mg/mL with 0.05mol/L HCl as an additive acid at 60°C for 15min. In this study, baseline separation of arsenic compounds could be achieved within 13min, except for phenylarsonic acid and phenylarsine oxide whose derivatized products are equal. The developed method was successfully validated and applied to 12 chicken muscle samples from Korean districts and other countries.
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Han Young
authors.initials: HY
authors.lastname: Eom
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Dong-Hyug
authors.initials: DH
authors.lastname: Yang
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Joon Hyuk
authors.initials: JH
authors.lastname: Suh
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Unyong
authors.initials: U
authors.lastname: Kim
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea.
authors.forename: Hyun-Deok
authors.initials: HD
authors.lastname: Cho
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, 221 Heukseok-Dong, Dongjak-Gu, Seoul 156-756, South Korea. Electronic address: hansb@cau.ac.kr.
authors.forename: Sang Beom
authors.initials: SB
authors.lastname: Han
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D016014:Linear Models; D018482:Muscle, Skeletal / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014050:Toluene / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D001152:Arsenicals; D014050:Toluene; C004848:dithiol
keywords: Arsenic compounds; Chicken muscle; Derivatization; Toluene-3,4-dithiol; Ultra-performance liquid chromatography-ultraviolet detection