title: Downregulation of MicroRNA-1 is Associated with Poor Prognosis in Hepatocellular Carcinoma.
abstract: BACKGROUND
  The relationship between microRNA-1 (miR-1) expression and prognosis has not been reported in hepatocellular carcinoma (HCC). The present study aimed to explore the clinicopathological significance and the prognostic role of miR-1 in HCC.
  
  
  METHODS
  The expression levels of miR-1 were quantified using real-time quantitative PCR (q-PCR) in 40 surgically resected HCC samples and matched adjacent non-cancerous tissues.
  
  
  RESULTS
  MiR-1 expression was significantly downregulated in HCC compared with matched non-cancerous tissues. Aberrant miR-1 expression was significantly correlated with gender, expression of hepatitis B virus surface antigen (HBsAg), tumor differentiation, vein invasion, and TNM stage. Patients with low expression of miR-1 had significantly reduced overall survival compared with patients with high expression of miR-1 (p = 0.04).The multivariate Cox regression analysis indicated that miR-1 expression (HR = 2.79; p = 0.005), gender (HR = 0.087; p = 0.005), vein invasion (HR = 0.172; p = 0.007), and TNM stage (HR = 3.421; p = 0.001) were independent prognostic factors for overall survival.
  
  
  CONCLUSIONS
  Low miR-1 expression is associated with shortened survival time. MiR-1 may act as a potential prognostic biomarker for HCC patients.
authors.forename: Xuanyi
authors.initials: X
authors.lastname: Wang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Huang
authors.forename: Haiwen
authors.initials: H
authors.lastname: Zhuang
authors.forename: Yayun
authors.initials: Y
authors.lastname: Qian
authors.forename: Qiu
authors.initials: Q
authors.lastname: Zhao
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Hao
authors.initials: H
authors.lastname: Gu
authors.forename: Jue
authors.initials: J
authors.lastname: Chen
authors.forename: Renhua
authors.initials: R
authors.lastname: Guo
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D002681:China / Q000453:epidemiology; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006509:Hepatitis B / Q000453:epidemiology; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D053208:Kaplan-Meier Estimate; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: C097353:AFP protein, human; D014408:Biomarkers, Tumor; D006514:Hepatitis B Surface Antigens; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D000509:alpha-Fetoproteins