title: Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer.
abstract: We compared the immunohistochemical expression of putrescine (PUT), spermine (SPM), ornithine decarboxylase (ODC), and diamine oxidase (DAO) in bioptic samples of canine colonic mucosa with chronic inflammation (i.e., granulomatous colitis and lymphoplasmacytic colitis) or neoplasia. Single and total polyamines levels were significantly higher in neoplastic tissue than in normal samples. Samples with different degrees of inflammation showed a general decrease expression of ODC if compared to controls; SPM was practically not expressed in control samples and very low in samples with chronic-granulomatous inflammation. In carcinomatous samples, the ODC activity was higher with respect to controls and samples with inflammation. This is the first description of polyamines expression in dog colonic mucosa in normal and in different pathological conditions, suggesting that the balance between polyamine degradation and biosynthesis is evidently disengaged during neoplasia.
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Rossi
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Cerquetella
authors.affiliation: S. Antonio Clinic, 26020 Madignano, Italy.
authors.forename: Graziano
authors.initials: G
authors.lastname: Pengo
authors.affiliation: School of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, Italy.
authors.forename: Subeide
authors.initials: S
authors.lastname: Mari
authors.affiliation: Faculty of Veterinary Medicine, 050097 Bucharest, Romania.
authors.forename: Emilia
authors.initials: E
authors.lastname: Balint
authors.affiliation: Gastroenterology and Hepatology Section, Department of Medicine, Santa Maria della Misericordia Hospital, Piazzale Menghini 1, San Sisto, 06153 Perugia, Italy.
authors.forename: Gabrio
authors.initials: G
authors.lastname: Bassotti
authors.affiliation: Faculty of Veterinary Medicine, 050097 Bucharest, Romania.
authors.forename: Nicolae
authors.initials: N
authors.lastname: Manolescu
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D002908:Chronic Disease; D003092:Colitis / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D004285:Dogs; D007413:Intestinal Mucosa / Q000378:metabolism*; D009928:Organ Specificity; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism*; D012016:Reference Values; D013045:Species Specificity; D013096:Spermine / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D013096:Spermine; D006631:Amine Oxidase (Copper-Containing); D009955:Ornithine Decarboxylase; D011700:Putrescine