title: Diallyl trisulfide inhibits naphthalene-induced oxidative injury and the production of inflammatory responses in A549 cells and mice.
abstract: Diallyl trisulfide (DATS) is a garlic organosulfide that may have a therapeutic potential in the treatment of some diseases. We sought to determine whether DATS could inhibit naphthalene-induced oxidative injury and the production of inflammatory responses in vitro and in vivo. A549 cells were either pre-treated (PreTx, prevention) or concurrently treated (CoTx, treatment) with 20μM naphthalene and either 5 or 10μM DATS. PreTx and CoTx showed the prevention and the treatment potential of DATS to inhibit the generation of naphthalene-induced reactive oxygen species (ROS) in the A549 cells. DATS showed antioxidative activity by elevating the SOD activities in the low dose groups. The mechanistic study showed that the DATS-mediated inhibition of naphthalene-induced oxidative injury and the production of inflammatory responses (i.e., TNF-α, IL-6, and IL-8) were attributed to inhibiting the activity of nuclear factor-kappa B (NF-κB). In addition, DATS inhibited the production of serum nitric oxide NO and myeloperoxidase (MPO) in the lungs of Kunming mice. The histological analysis results indicate that DATS inhibited the naphthalene-induced lung damage, which is consistent with the in vitro study results. The in vivo and in vitro results suggest that DATS may be an effective attenuator of naphthalene-induced lung damage.
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China; School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Daxue Road, Western University Science Park, Jinan, Shandong 250353, PR China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
authors.forename: Guimei
authors.initials: G
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China; Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, PR China. Electronic address: uszxzhao@gmail.com.
authors.forename: Zhongxi
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, PR China.
authors.forename: Shanzhong
authors.initials: S
authors.lastname: Li
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, PR China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cai
authors.affiliation: Jiangsu Shengshi Kangde Biotech Corporation, Lianyungang, Jiangsu 222006, PR China.
authors.forename: Jimin
authors.initials: J
authors.lastname: Cao
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D009281:Naphthalenes / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013440:Sulfides / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000498:Allyl Compounds; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D018836:Inflammation Mediators; D016328:NF-kappa B; D009281:Naphthalenes; D017382:Reactive Oxygen Species; D013440:Sulfides; C042577:diallyl trisulfide; C031721:naphthalene; D009569:Nitric Oxide; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: Diallyl trisulfide; Inflammatory responses; Naphthalene; Nuclear factor-kappa B (NF-κB); Oxidative injury