title: Immunostimulation by phospholipopeptide biosurfactant from Staphylococcus hominis in Oreochromis mossambicus.
abstract: The immunostimulatory effect of phospholipopeptide biosurfactant from Staphylococcus hominis (GenBank Accession No: KJ564272) was assessed with Oreochromis mossambicus. The non-specific (serum lysozyme activity, serum antiprotease activity, serum peroxidase activity and serum bactericidal activity), specific (bacterial agglutination assay) immune responses and disease resistance activity against Aeromonas hydrophila were examined. Fish were intraperitonially injected with water soluble secondary metabolite (biosurfactant) of S. hominis at a dose of 2 mg, 20 mg and 200 mg kg(-1) body weight. Commercial surfactant surfactin (sigma) at 20 mg kg(-1) was used as standard and saline as negative control. All the doses of water soluble biosurfactant tested, significantly enhanced the specific, nonspecific immunity and disease resistance from the day of post administration of phospholipopeptide biosurfactant till the tail of the experimental period. These results clearly indicated that the secondary metabolite isolated from S. hominis stimulates the immunity of finfish thereby could enhance aquaculture production.
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Veluchamy
authors.initials: V
authors.lastname: Rajeswari
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Sekaran
authors.initials: S
authors.lastname: Kalaivani Priyadarshini
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Viswanathan
authors.initials: V
authors.lastname: Saranya
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India.
authors.forename: Ponnusamy
authors.initials: P
authors.lastname: Suguna
authors.affiliation: Postgraduate and Research Department of Zoology and Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India. Electronic address: shenbagarathai@gmail.com.
authors.forename: Rajaiah
authors.initials: R
authors.lastname: Shenbagarathai
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology; D000372:Agglutination Tests; D000818:Animals; D017756:Aquaculture; D060467:Disease Resistance; D005393:Fish Diseases / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D007114:Immunization; D008074:Lipoproteins / Q000096:biosynthesis / Q000276:immunology*; D009113:Muramidase / Q000097:blood; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D009195:Peroxidase / Q000097:blood; D011480:Protease Inhibitors / Q000097:blood; D041181:Staphylococcus hominis / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism; D017210:Tilapia / Q000097:blood / Q000276:immunology*
chemical_list: D008074:Lipoproteins; D010455:Peptides; D011480:Protease Inhibitors; D013501:Surface-Active Agents; D009195:Peroxidase; D009113:Muramidase
keywords: Disease resistance; Immunostimulant; Non-specific immune response; Phospholipopeptide biosurfactant; Secondary metabolite; Specific immune response