title: Short-term exposure to benzo[a]pyrene causes oxidative damage and affects haemolymph steroid levels in female crab Portunus trituberculatus.
abstract: Concern has increased regarding the adverse effects of polycyclic aromatic hydrocarbons (PAHs) on reproduction. However, limited information is available on the effects of PAHs in crustacean. In order to determine whether benzo[a]pyrene (B[a]P) could cause reproductive toxicity on the swimming crab Portunus trituberculatus, sexually mature female crabs were exposed to environmentally relevant concentrations of B[a]P (0, 0.1, 0.5 and 2.5 μg/L) for 10 days. B[a]P treatments resulted in high accumulation in ovary, and induced oxidative stress in a dose-dependent manner on ovary of crab. Furthermore, the haemolymph estradiol (E2) and testosterone (T) levels were significantly decreased. Histological investigation also revealed the reproductive toxicity caused by B[a]P. The results demonstrated that waterborne exposure to B[a]P caused oxidative damage and disrupted sex steroids in female crab P. trituberculatus, ultimately resulting in histological alternation.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wen
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D003386:Brachyura / Q000502:physiology; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006458:Hemolymph / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011084:Polycyclic Aromatic Hydrocarbons; D012098:Reproduction; D013256:Steroids / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D013256:Steroids; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D013739:Testosterone; D004958:Estradiol
keywords: Benzo[a]pyrene; Ovary; Oxidative damage; Portunus trituberculatus; Steroids levels