title: Preparation of SELEX Samples for Next-Generation Sequencing.
abstract: Fuelled by massive whole genome sequencing projects such as the human genome project, enormous technological advancements and therefore tremendous price drops could be achieved, rendering next-generation sequencing very attractive for deep sequencing of SELEX libraries. Herein we describe the preparation of SELEX samples for Illumina sequencing, based on the already established whole genome sequencing workflow. We describe the addition of barcode sequences for multiplexing and the adapter ligation, avoiding associated pitfalls.
authors.affiliation: Life & Medical Sciences Institute (LIMES), University of Bonn, Gerhard-Domagk-Str. 1, Bonn, 53121, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Tolle
authors.affiliation: Life & Medical Sciences Institute (LIMES), University of Bonn, Gerhard-Domagk-Str. 1, Bonn, 53121, Germany. gmayer@uni-bonn.de.
authors.forename: Günter
authors.initials: G
authors.lastname: Mayer
mesh_terms: D052157:Aptamers, Nucleotide; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide
keywords: Aptamer; High-throughput sequencing; Illumina dye sequencing; Next-generation sequencing; SELEX