title: Pregnancy-Induced ISG-15 and MX-1 Gene Expression is Detected in the Liver of Holstein-Friesian Heifers During Late Peri-Implantation Period.
abstract: The bovine embryonic signal interferon-τ (IFN-τ) produced by the trophoblast is known to pass through the uterine fluid towards the endometrium and further into the maternal blood, where IFN-τ induces specific expression of interferon-stimulated gene expression (ISG), for example in peripheral leucocytes. In sheep, it was shown experimentally by administration of IFN-τ that ISG is also detectable in the liver. The objective was to test whether ISG can be detected in liver biopsy specimens from Holstein-Friesian heifers during early pregnancy. Liver biopsies were taken on day 18 from pregnant and non-pregnant heifers (n = 19), and the interferon-stimulated protein 15 kDa (ISG-15) and myxovirus-resistance protein-1 (MX-1) gene expression was detected. The expression of both MX-1 (p: 24.33 ± 7.40 vs np: 9.00 ± 4.02) and ISG-15 (p: 43.73 ± 23.22 vs 7.83 ± 3.63) was higher in pregnant compared to non-pregnant heifers (p < 0.05). In conclusion, pregnancy induced ISG-15 and MX-1 gene expression in the liver already at day 18 in cattle.
authors.affiliation: Clinic for Cattle, Endocrinology Laboratory, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: M M
authors.initials: MM
authors.lastname: Meyerholz
authors.affiliation: Clinic for Cattle, Endocrinology Laboratory, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Mense
authors.affiliation: Clinic for Cattle, Endocrinology Laboratory, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Knaack
authors.affiliation: National Institute for Agricultural Research, UMR1198 Biologie du Développement et Reproduction, INRA, Jouy-en-Josas, France.
authors.forename: O
authors.initials: O
authors.lastname: Sandra
authors.affiliation: Clinic for Cattle, Endocrinology Laboratory, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schmicke
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D005865:Gestational Age; D007370:Interferon Type I / Q000494:pharmacology / Q000502:physiology; D008099:Liver / Q000737:chemistry*; D064547:Myxovirus Resistance Proteins / Q000235:genetics*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D014327:Trophoblasts / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics*
chemical_list: D007370:Interferon Type I; D064547:Myxovirus Resistance Proteins; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D014452:Ubiquitins; C020947:interferon tau