title: Form of supplemental selenium fed to cycling cows affects systemic concentrations of progesterone but not those of estradiol.
abstract: In areas where soils are deficient in selenium (Se), dietary supplementation of this trace mineral directly to cattle is recommended. Selenium status affects fertility, and the form of Se supplemented to cows affects tissue-specific gene expression profiles. The objective of this study was to determine whether the form of Se consumed by cows would affect follicular growth and the production of steroids. Thirty-three Angus-cross cows that had ad libitum access of a mineral mix containing 35 ppm of Se in free-choice vitamin-mineral mixes as either inorganic (ISe), organic (OSe), or a 50/50 mix of ISe and OSe (MIX) for 180 days were used. After 170 days of supplementation, all cows were injected with 25-mg PGF2α to induce regression of the CL and then monitored for behavioral estrus (Day 0). From Day 4 to Day 8 after estrus, follicular growth was determined by transrectal ultrasonography. On Day 6, cows were injected with PGF2α (20 then 15 mg, 8-12 hours apart) to induce regression of the developing CL and differentiation of the dominant follicle of the first follicular wave into a preovulatory follicle. On Day 8, 36 hours after PGF2α (20 mg), the contents of the preovulatory follicle were aspirated by ultrasound-guided follicular puncture. Blood collected on Days 6 and 8 and follicular fluid collected on Day 8 was analyzed for concentrations of progesterone and estradiol. Form of Se supplemented to cows affected (P = 0.04) the systemic concentration of progesterone on Day 6, but not on Day 8. Form of Se did not affect the systemic concentration of estradiol on Day 6 or Day 8. Form of Se tended to affect (P = 0.07) the concentration of progesterone, but not that of estradiol, in the follicular fluid. Form of Se did not affect diameter of the dominant ovarian follicle on Days 4 to 6, but tended to affect (P = 0.08) the diameter of the preovulatory follicle on Day 8. Our results suggest that form of Se fed to cows affects the production of progesterone but not that of estradiol. Further investigation of organic Se-induced increases in progesterone and potentially the effects of increased progesterone on the establishment of pregnancy, especially in cows of lower fertility, is warranted.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Katheryn L
authors.initials: KL
authors.lastname: Cerny
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Les
authors.initials: L
authors.lastname: Anderson
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: Walter R
authors.initials: WR
authors.lastname: Burris
authors.affiliation: Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Rhoads
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Matthews
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, Kentucky, USA. Electronic address: phillip.bridges@uky.edu.
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Bridges
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D004304:Dosage Forms; D004958:Estradiol / Q000032:analysis / Q000097:blood*; D030762:Estrous Cycle / Q000097:blood / Q000187:drug effects; D004973:Estrus Synchronization / Q000379:methods; D005260:Female; D005298:Fertility / Q000187:drug effects; D015571:Follicular Fluid / Q000737:chemistry / Q000187:drug effects; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D011374:Progesterone / Q000032:analysis / Q000097:blood*; D012643:Selenium / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D004304:Dosage Forms; D011374:Progesterone; D004958:Estradiol; D012643:Selenium
keywords: CL; Cattle; Follicle; Selenium; Steroidogenesis