title: Method development and validation of potent pyrimidine derivative by UV-VIS spectrophotometer.
abstract: BACKGROUND
  A rapid and sensitive ultraviolet-visible (UV-VIS) spectroscopic method was developed for the estimation of pyrimidine derivative 6-Bromo-3-(6-(2,6-dichlorophenyl)-2-(morpolinomethylamino) pyrimidine4-yl) -2H-chromen-2-one (BT10M) in bulk form.
  
  
  RESULTS
  Pyrimidine derivative was monitored at 275 nm with UV detection, and there is no interference of diluents at 275 nm. The method was found to be linear in the range of 50 to 150 μg/ml. The accuracy and precision were determined and validated statistically. The method was validated as a guideline.
  
  
  CONCLUSIONS
  The results showed that the proposed method is suitable for the accurate, precise, and rapid determination of pyrimidine derivative. Graphical Abstract Method development and validation of potent pyrimidine derivative by UV spectroscopy.
authors.affiliation: NIMS University, Shobha Nagar, Jaipur, 303001, Rajasthan, India. anshu_17oct@yahoo.co.in.|Vishveshwarya Institute of Medical Science, Gautambudh Nagar, 203207, Uttar Pradesh, India. anshu_17oct@yahoo.co.in.
authors.forename: Anshu
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: NIMS University, Shobha Nagar, Jaipur, 303001, Rajasthan, India. anoop_bajrang10@yahoo.co.in.
authors.forename: Anoop
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Pharmaceutical Sciences, M.D. University, Rohtak, 124001, Haryana, India. vermapk422@rediffmail.com.
authors.forename: Prabhakar Kumar
authors.initials: PK
authors.lastname: Verma
keywords: Derivative; Pyrimidine; UV-VIS spectroscopy; Validation