title: Mechanistic Study of the Phytocompound, 2- β -D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches.
abstract: Bidens pilosa, a medicinal herb worldwide, is rich in bioactive polyynes. In this study, by using high resolution 2-dimensional gel electrophoresis coupled with mass spectrometry analysis, as many as 2000 protein spots could be detected and those whose expression was specifically up- or downregulated in Jurkat T cells responsive to the treatment with 2-β-D-glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne (GHTT) can be identified. GHTT treatment can upregulate thirteen proteins involved in signal transduction, detoxification, metabolism, energy pathways, and channel transport in Jurkat cells. Nine proteins, that is, thioredoxin-like proteins, BH3 interacting domain death agonist (BID protein involving apoptosis), methylcrotonoyl-CoA carboxylase beta chain, and NADH-ubiquinone oxidoreductase, were downregulated in GHTT-treated Jurkat cells. Further, bioinformatics tool, Ingenuity software, was used to predict signaling pathways based on the data obtained from the differential proteomics approach. Two matched pathways, relevant to mitochondrial dysfunction and apoptosis, in Jurkat cells were inferred from the proteomics data. Biochemical analysis further verified both pathways involving GHTT in Jurkat cells. These findings do not merely prove the feasibility of combining proteomics and bioinformatics methods to identify cellular proteins as key players in response to the phytocompound in Jurkat cells but also establish the pathways of the proteins as the potential therapeutic targets of leukemia.
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan ; Institute of Biotechnology, National Taiwan University, 4F, No. 81, Chang-Xing Street, Taipei, Taiwan.
authors.forename: Jeng-Yuan
authors.initials: JY
authors.lastname: Shiau
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Yin
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Chang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Yi-Jou
authors.initials: YJ
authors.lastname: Hsu
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Kai-Wei
authors.initials: KW
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Tien-Fen
authors.initials: TF
authors.lastname: Kuo
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan.
authors.forename: Ching-Shan
authors.initials: CS
authors.lastname: Feng
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan ; Institute of Biotechnology, National Taiwan University, 4F, No. 81, Chang-Xing Street, Taipei, Taiwan.
authors.forename: Ning-Sun
authors.initials: NS
authors.lastname: Yang
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan ; Institute of Biotechnology, National Taiwan University, 4F, No. 81, Chang-Xing Street, Taipei, Taiwan.
authors.forename: Lie-Fen
authors.initials: LF
authors.lastname: Shyur
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, Taiwan ; Institute of Biotechnology, National Taiwan University, 4F, No. 81, Chang-Xing Street, Taipei, Taiwan ; Department of Life Sciences, National Chung Hsing University, Life Sciences Building 2F-7F, 250 Kuo Kuang Road, Taichung, Taiwan.
authors.forename: Wen-Chin
authors.initials: WC
authors.lastname: Yang
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
authors.forename: Tuan-Nan
authors.initials: TN
authors.lastname: Wen