title: A simple cytogenetic method to detect chromosomally integrated human herpesvirus-6.
abstract: Some healthy individuals carry human herpesvirus-6 (HHV-6) within a host chromosome, which is called inherited chromosomally integrated human herpesvirus-6 (iciHHV-6). Because iciHHV-6 is generally considered a non-pathogenic condition, it is important to distinguish iciHHV-6 from HHV-6 reactivation in immunocompromised hosts because both conditions manifest high copy numbers of the HHV-6 in peripheral blood mononuclear cells. Although fluorescent in situ hybridization (FISH) is a reliable method for the diagnosis of iciHHV-6, HHV-6-specific FISH probes are not commercially available. In our present study, we established a simple PCR-based method for producing FISH probes that can detect the chromosomal integration site of iciHHV-6 at high sensitivity. Using these probes, we confirmed that HHV-6 signals were consistently located at the telomeric region in all of the 13 iciHHV-6 individuals examined. Interestingly, in all seven Japanese iciHHV-6A patients, signals were detected exclusively on chromosome 22q. This method provides a simple and fast approach for iciHHV-6 diagnosis in the clinical laboratory.
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan; Department of Clinical Hematology, Faculty of Medical Technology, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tamae
authors.initials: T
authors.lastname: Ohye
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
authors.forename: Hidehito
authors.initials: H
authors.lastname: Inagaki
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Yoshikawa
authors.affiliation: Faculty of Clinical Engineering, Fujita Health University School of Health Sciences, Toyoake, Aichi 470-1192, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ihira
authors.affiliation: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.affiliation: Division of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan. Electronic address: kura@fujita-hu.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kurahashi
mesh_terms: D020732:Cytogenetic Analysis / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics; D015654:Herpesvirus 6, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D016662:Virus Integration
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes
keywords: Chromosomally integrated human herpesvirus-6; Chromosome; FISH; HHV-6 probe; PCR-based method; Telomere