title: Feed supplemented with polyphenolic byproduct from olive mill wastewater processing improves the redox status in blood and tissues of piglets.
abstract: In the present study, a polyphenolic byproduct from olive mill wastewater (OMWW) was used for making piglet feed with antioxidant activity. For examining the antioxidant capacity of the feed, 30 piglets of 20 d old were divided into two groups receiving basal or experimental feed for 30 d. Blood and tissue samples were drawn at days 2, 20, 35 and 50 post-birth. The tissues collected were brain, heart, kidney, liver, lung, quadriceps muscle, pancreas, spleen and stomach. The antioxidant effects of the experimental feed were assessed by measuring oxidative stress biomarkers in blood and tissues. The oxidative stress markers were total antioxidant capacity (TAC), glutathione (GSH), catalase activity (CAT), protein carbonyls (CARB) and thiobarbituric acid reactive species (TBARS). The results showed that piglets fed with diet supplemented with OMWW polyphenols had significantly increased antioxidant mechanisms in blood and the majority of the tested tissues as shown by increases in TAC, CAT and GSH compared to control group. Moreover, piglets fed with the experimental feed exhibited decreased oxidative stress-induced damage to lipids and proteins as shown by decreases in TBARS and CARB respectively. This is the first study in which OMWW polyphenols were used for making pig feed with antioxidant activity.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou, 41221 Larissa, Greece; Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Gerasopoulos
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou, 41221 Larissa, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Petrotos
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
authors.forename: Stylianos
authors.initials: S
authors.lastname: Kokkas
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kantas
authors.affiliation: Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Goulas
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 and Aiolou, 41221 Larissa, Greece. Electronic address: dkouret@uth.gr.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kouretas
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D015415:Biomarkers; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005511:Food Handling; D007220:Industrial Waste; D031658:Olea; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D059808:Polyphenols / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013552:Swine / Q000097:blood*; D013997:Time Factors; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D007220:Industrial Waste; D059808:Polyphenols; D062065:Wastewater
keywords: Antioxidant feed; Ceramic membrane microfiltration; Natural products; Olive mill wastewater; Oxidative stress; Piglet