title: A novel N-acetyl-glucosamine lectin of Lonchocarpus araripensis attenuates acute cellular inflammation in mice.
abstract: OBJECTIVE
  This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis.
  
  
  METHODS
  LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25-30 g; n = 6/group) in models of paw edema and peritonitis.
  
  
  METHODS
  ANOVA (p < 0.05).
  
  
  RESULTS
  LAL revealed two bands of 30 and 60 kDa (SDS-PAGE) and exhibited hemagglutinating activity. LAL (10 mg/kg) inhibited the paw edema (77%) and vascular permeability (26%) induced by carrageenan, and the paw edema induced by serotonin (80%), bradykinin (49%), sodium nitroprusside (83%), TNF-α (75%) and PGE2 (64%). LAL also inhibited the neutrophil migration induced by fMLP (70%) or carrageenan (69%). The intravital microscopy showed that LAL inhibited rolling (83%) and adhesion (70%) of leukocytes. LAL anti-inflammatory effect was reversed by its association with N-acetyl-glucosamine. The nine-daily treatment with LAL (10 mg/kg; i.v.) showed no toxicity.
  
  
  CONCLUSIONS
  The novel N-acetyl-D-glucosamine-binding lectin isolated from L. araripensis seeds presents anti-inflammatory effect involving the lectin domain and the inhibition of 5-HT, BK, PGE2, NO, TNF-α and leukocyte rolling and adhesion.
authors.affiliation: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil. alana_pires@yahoo.com.br.|Centro de Ciências da Saúde, Unidade Via Corpvs, Centro Universitário Estácio do Ceará, Rua Eliseu Uchoa Becco, 600, Fortaleza, CE, 60.810-270, Brazil. alana_pires@yahoo.com.br.
authors.forename: Alana F
authors.initials: AF
authors.lastname: Pires
authors.affiliation: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil.
authors.forename: Natália V F C
authors.initials: NV
authors.lastname: Rodrigues
authors.affiliation: Departamento de Morfologia e Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Delmiro de Farias, s/n, Rodolfo Teófilo, Fortaleza, CE, 60.416-030, Brazil.
authors.forename: Pedro Marcos G
authors.initials: PM
authors.lastname: Soares
authors.affiliation: Departamento de Morfologia e Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Delmiro de Farias, s/n, Rodolfo Teófilo, Fortaleza, CE, 60.416-030, Brazil.
authors.forename: Ronaldo de Albuquerque
authors.initials: Rde A
authors.lastname: Ribeiro
authors.affiliation: Departamento de Morfologia e Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Delmiro de Farias, s/n, Rodolfo Teófilo, Fortaleza, CE, 60.416-030, Brazil.
authors.forename: Karoline S
authors.initials: KS
authors.lastname: Aragão
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Mister Hull, s/n, Fortaleza, CE, 60.451-970, Brazil.
authors.forename: Márcia M
authors.initials: MM
authors.lastname: Marinho
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Mister Hull, s/n, Fortaleza, CE, 60.451-970, Brazil.
authors.forename: Mayara T L
authors.initials: MT
authors.lastname: da Silva
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Mister Hull, s/n, Fortaleza, CE, 60.451-970, Brazil.
authors.forename: Benildo S
authors.initials: BS
authors.lastname: Cavada
authors.affiliation: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil. anassreuy@gmail.com.
authors.forename: Ana Maria S
authors.initials: AM
authors.lastname: Assreuy
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004912:Erythrocytes / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D006384:Hemagglutination / Q000187:drug effects; D066298:In Vitro Techniques; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control*; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000517:prevention & control; D011817:Rabbits; D012639:Seeds / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D037102:Lectins; D000117:Acetylglucosamine
keywords: Acute inflammation; Leguminous lectin; Leukocyte; Lonchocarpus araripensis; N-acetyl-glucosamine