title: Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation.
abstract: Due to the low proliferative and migratory capacities of chondrocytes, cartilage repair remains a challenging clinical problem. Current therapeutic strategies for cartilage repair result in unsatisfactory outcomes. Autologous chondrocyte implantation (ACI) is a cell based therapy that relies on the in vitro expansion of healthy chondrocytes from the patient, during which proliferation‑promoting factors are frequently used. Neuroleukin (NLK) is a multifunctional protein that possesses growth factor functions, and its expression has been associated with cartilage development and bone regeneration, however its direct role in chondrocyte proliferation remains to be fully elucidated. In the current study, the role of NLK in chondrocyte proliferation in vitro in addition to its potential to act as an exogenous factor during ACI was investigated. Furthermore, the concentration of NLK for in vitro chondrocyte culture was optimized using a microfluidic device. An NLK concentration of 12.85 ng/ml was observed to provide optimal conditions for the promotion of chondrocyte proliferation. Additionally, NLK stimulation resulted in an increase in type II collagen synthesis by chondrocytes, which is a cartilaginous secretion marker and associated with the phenotype of chondrocytes. Together these data suggest that NLK is able to promote cell proliferation and type II collagen synthesis during in vitro chondrocyte propagation, and thus may serve as an exogenous factor for ACI.
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Kang
authors.initials: K
authors.lastname: Tian
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yingqiu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Yin
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Institute of Cancer Stem Cell, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D024043:Collagen Type II / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004867:Equipment Design; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D008297:Male; D044085:Microfluidics / Q000379:methods*; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D024043:Collagen Type II; D016207:Cytokines; C554959:GPI protein, human; D005956:Glucose-6-Phosphate Isomerase