title: Healing of skin wounds with a chitosan-gelatin sponge loaded with tannins and platelet-rich plasma.
abstract: A chitosan-gelatin sponge (CSGT) was prepared using a chitosan/ascorbic acid solution blend containing gelatin, followed by crosslinking with tannin acid and freeze-drying, thereby combining the chitosan sponge and gelatin sponge. The structure of the CSGT was observed by scanning electron microscopy and was shown to have uniform and abundant pores measuring about 145-240μm in size. We also characterized the sponges by infrared spectroscopy, thermogravimetric analysis, mechanical property tests, swelling behavior analysis, water retention capacity tests, antibacterial property analysis, and cytotoxicity tests. Our data showed that the CSGT had good thermostability and mechanical properties as well as efficient water absorption and retention capacities. Moreover, the CSGT could effectively inhibit the growth of Escherichia coli and Staphylococcus aureus with low toxicity. In animal experiments, macroscopic observations and histological examinations showed that the wound covered by the CSGT healed quickly. Additionally, loading of the CSGT with platelet-rich plasma resulted in further acceleration of wound healing. Therefore, the CSGT and the CSGT with platelet-rich plasma were suitable for application as a wound dressing and may have potential for use in various biomedical applications.
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Bitao
authors.initials: B
authors.lastname: Lu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Tianyou
authors.initials: T
authors.lastname: Wang
authors.affiliation: The Ninth People's Hospital of Chongqing, Chongqing 400715, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
authors.forename: Fangying
authors.initials: F
authors.lastname: Dai
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Lv
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Tang
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China. Electronic address: 30353930@qq.com.
authors.forename: Guangqian
authors.initials: G
authors.lastname: Lan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry*; D055595:Mechanical Phenomena; D051379:Mice; D008826:Microbial Sensitivity Tests; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D016062:Porosity; D012867:Skin / Q000187:drug effects* / Q000473:pathology*; D017550:Spectroscopy, Fourier Transform Infrared; D013634:Tannins / Q000008:administration & dosage* / Q000633:toxicity; D013816:Thermodynamics; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D013634:Tannins; D005780:Gelatin; D048271:Chitosan
keywords: Chitosan; Platelet-rich plasma; Sponge scaffold; Wound healing