title: Sensing and antibacterial activity of imidazolium-based conjugated polydiacetylenes.
abstract: In the current study, we report the first example of polydiacetylenes (PDAs), where our PDA-based system acts as both a sensing probe and killer for bacteria. The contact of imidazolium and imidazole-derived PDA with various bacterial strains including MRSA (methicillin-resistant Staphylococcus aureus) and ESBL-EC (extended-spectrum β-lactamase-producing Escherichia coli) results in a distinct blue-to-red colorimetric change of the solution as well as a rapid disruption of the bacterial membrane, which is demonstrated by transmission electron microscopy and confocal microscopy. Zeta potential analysis supports that antibacterial activity of the PDA solution originates from an electrostatic interaction between the negatively charged bacterial cell surface and the positively charged polymers. These results suggest that the PDA has a great potential to carry out the dual roles of a probe and killer for bacteria.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Songyi
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea.
authors.forename: Meiying
authors.initials: M
authors.lastname: Chi
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: xuql@ewha.ac.kr.
authors.forename: Qingling
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing 210-009, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Seoul Center, Korea Basic Science Institute, Seoul 136-713, Republic of Korea.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Eom
authors.affiliation: Department of Chemistry, University of Bath, Bath BA2-7AY, UK.
authors.forename: Tony D
authors.initials: TD
authors.lastname: James
authors.affiliation: School of Mechanical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea. Electronic address: nanopark@skku.edu.
authors.forename: Sungsu
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Republic of Korea. Electronic address: jyoon@ewha.ac.kr.
authors.forename: Juyoung
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000138:chemical synthesis; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage / Q000737:chemistry; D008422:Materials Testing; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000008:administration & dosage* / Q000138:chemical synthesis; D053279:Polyynes / Q000008:administration & dosage* / Q000138:chemical synthesis; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000302:isolation & purification* / Q000502:physiology; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D007093:Imidazoles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; C029899:imidazole
keywords: Antibacterial effect; Colorimetric sensor; Fluorescent sensor; Polydiacetylenes; Probe-killer