title: The Effect of Silencing HIF-1α Gene in BxPC-3 Cell Line on Glycolysis-Related Gene Expression, Cell Growth, Invasion, and Apoptosis.
abstract: Hypoxia has been proved to be a typical character of solid tumors. Tumor cells prefer to use glucose through the glycolysis pathway instead of aerobic respiration. However, the precise molecular mechanism underlying this so-called Warburg effect remains elusive. In the current study, siRNA was synthesized and transfected into BxPC-3 cell line to silence the expression of HIF-1α gene. It was found that hypoxia induced hypoxia-inducible factor 1α (HIF-1α) overexpression in BxPC-3 cells, enhanced the expression of pyruvate dehydrogenase kinase 1 and lactate dehydrogenase A, thus facilitating glycolysis and making tumor cells more tolerant to hypoxic stress. The silencing of HIF-1α gene significantly attenuated glycolysis under hypoxic conditions, inhibited the growth and invasion ability of BxPC-3 cells, and enhanced hypoxia-induced cell apoptosis.
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Guo-Hao
authors.initials: GH
authors.lastname: Wu
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: He
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Zhuang
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Qiu-Lei
authors.initials: QL
authors.lastname: Xi
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: a Department of General Surgery , Zhongshan Hospital, Fudan University , Shanghai , China.
authors.forename: Yu-Song
authors.initials: YS
authors.lastname: Han
authors.affiliation: b Key Laboratory of Nutrition and Metabolisms, Institute of Nutritional Sciences, SIBS, Chinese Academy of Sciences , Shanghai , China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing; D006019:Glycolysis / Q000235:genetics*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000502:physiology*; D007527:Isoenzymes / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D000080864:Lactate Dehydrogenase 5; D019344:Lactic Acid / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007527:Isoenzymes; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D034741:RNA, Small Interfering; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; D017346:Protein Serine-Threonine Kinases