title: Using the inherent chemistry of the endothelin-1 peptide to develop a rapid assay for pre-transplant donor lung assessment.
abstract: Endothelin-1 is a potent vasoconstrictive peptide that plays an important role in ex vivo lung perfusion. ET-1 expression levels are predictive of lung transplant outcomes and represent a valuable monitoring tool for surgeons; however, traditional techniques that measure [ET-1] are not suitable for the transplant setting. Herein, we demonstrate a new assay that rapidly measures ET-1 peptide levels in lung perfusate.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, Toronto, ON, CanadaM5S 3M2. shana.kelley@utoronto.ca.
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Sage
authors.affiliation: Division of Thoracic Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada and Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, ON, CanadaM5G 1L7.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Bai
authors.affiliation: Division of Thoracic Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada and Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, ON, CanadaM5G 1L7.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Cypel
authors.affiliation: Division of Thoracic Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada and Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, ON, CanadaM5G 1L7.
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Division of Thoracic Surgery, University of Toronto, Toronto, ON M5G 1L7, Canada and Latner Thoracic Surgery Research Laboratories, Toronto General Research Institute, University Health Network, Toronto, ON, CanadaM5G 1L7.
authors.forename: Shaf
authors.initials: S
authors.lastname: Keshavjee
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Toronto, Toronto, ON, CanadaM5S 3M2. shana.kelley@utoronto.ca and Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, ON M5S 3M2, Canada.
authors.forename: Shana O
authors.initials: SO
authors.lastname: Kelley
mesh_terms: D001681:Biological Assay; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D019332:Endothelin-1 / Q000737:chemistry*; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D016040:Lung Transplantation; D010455:Peptides / Q000737:chemistry*; D010477:Perfusion; D013997:Time Factors
chemical_list: D019332:Endothelin-1; D010455:Peptides