title: Protective effect of oleanolic acid on oxidized-low density lipoprotein induced endothelial cell apoptosis.
abstract: Oleanolic acid (3β-hydroxyolean-12-en-28-oic acid, OA) is a naturally-occurring triterpenoid with various promising pharmacological properties. The present study was conducted to determine the protective effects of OA against oxidized low-density lipoprotein (ox-LDL) induced endothelial cell apoptosis and the possible underlying mechanisms. Our results showed that ox-LDL significantly decreased cell viability and induced apoptosis in human umbilical vein endothelial cells (HUVECs). OA in the co-treatment showed a protective effect against ox-LDL induced loss in cell viability and an increase in apoptosis, which was associated with the modulating effect of OA on ox-LDL induced hypoxia-inducible factor 1α(HIF-1α) expression. Moreover, our results showed that the modulating effect of OA against ox-LDL induced HIF-1α expression was obtained via inhibition of lipoprotein receptor 1 (LOX-1)/reactive oxygen species (ROS) signaling. Collectively, we suggested that the protective effect of OA against ox-LDL induced HUVEC apoptosis might, at least in part, be obtained via inhibition of the LOX-1/ROS/HIF-1α signaling pathway.
authors.affiliation: Department of Pharmacy, the Third People's Hospital of Qingdao.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Guanghui
authors.initials: G
authors.lastname: Li
authors.forename: Meizhi
authors.initials: M
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Han
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D050197:Atherosclerosis / Q000517:prevention & control*; D004353:Drug Evaluation, Preclinical; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008077:Lipoproteins, LDL; D009828:Oleanolic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051127:Scavenger Receptors, Class E / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; D010936:Plant Extracts; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; D009828:Oleanolic Acid