title: Modulation of cytotoxic and genotoxic effects of nanoparticles in cancer cells by external magnetic field.
abstract: Magnetic nanoparticles are well known for anticancer activity by deregulating cellular functions. In the present study, cellular effects of low strength static magnetic field (SMF) were explored. How nanoparticles affect the cellular response in presence and absence of static magnetic field was also studied. Peripheral blood mononuclear cells (PBMC) and human lymphoma monocytic cell line U937 were chosen as representative normal and cancer cells models. The two effects we would like to report in this paper are, DNA damage induced by SMF of the order of 70 mT, and alteration in membrane potential. The other notable aspect was the changes were diametrically opposite in normal and cancer cell types. DNA damage was observed only in cancer cells whereas membrane depolarization was observed in normal cells. Iron oxide nanoparticles (IONP) and gold nanoparticles (AuNP) were also used for cellular response studies in presence and absence of SMF. The effects of the magnetic nanoparticle IONP and also of AuNP were sensitive to presence of SMF. Unlike cancer cells, normal cells showed a transient membrane depolarization sensitive to static magnetic field. This depolarization effect exclusive for normal cells was suggested to have correlations with their higher repair capacity and lesser propensity for DNA damage. The work shows cancer cells and normal cells respond to nanoparticle and static magnetic field in different ways. The static magnetic induced DNA damage observed exclusively in cancer cells may have therapeutic implications. From the conclusions of the present investigation we may infer that static magnetic field enhances the therapeutic potentials of nanoparticles. Such low strength magnetic field seems to be a promising external manoeuvring agent in designing theranostics.
authors.affiliation: Department of Biochemistry and Centre for excellence in Biomedical Engineering and Systems Biology, University of Calcutta, Kolkata, 700019 India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Shaw
authors.affiliation: Department of Biochemistry and Centre for excellence in Biomedical Engineering and Systems Biology, University of Calcutta, Kolkata, 700019 India.
authors.forename: Sufi O
authors.initials: SO
authors.lastname: Raja
authors.affiliation: Department of Biochemistry and Centre for excellence in Biomedical Engineering and Systems Biology, University of Calcutta, Kolkata, 700019 India.
authors.forename: Anjan Kr
authors.initials: AK
authors.lastname: Dasgupta
keywords: Cell viability; DNA damage; Magnetic nanoparticle; Membrane potential; Normal cell and cancer cell; Static magnetic field