title: Antioxidant status of turkey breast meat and blood after feeding a diet enriched with histidine.
abstract: The objective of this study was to investigate the effects of 1) spray dried blood cells rich in histidine and 2) pure histidine added to feed on the antioxidant status and concentration of carnosine related components in the blood and breast meat of female turkeys. The experiment was performed on 168 Big7 turkey females randomly assigned to 3 dietary treatments: control; control with the addition of 0.18% L-histidine (His); and control with the addition of spray dried blood cells (SDBC). Birds were raised for 103 d on a floor with sawdust litter, with drinking water and feed ad libitum. The antioxidant status of blood plasma and breast muscle was analyzed by ferric reducing ability (FRAP) and by 2,2-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging ability. The activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) was analyzed in the blood and breast meat, with the content of carnosine and anserine quantified by HPLC. Proximate analysis as well as amino acid profiling were carried out for the feed and breast muscles. Growth performance parameters also were calculated. Histidine supplementation of the turkey diet resulted in increased DPPH radical scavenging capacity in the breast muscles and blood, but did not result in higher histidine dipeptide concentrations. The enzymatic antioxidant system of turkey blood was affected by the diet with SDBC. In the plasma, the SDBC addition increased both SOD and GPx activity, and decreased GPx activity in the erythrocytes. Feeding turkeys with an SDBC containing diet increased BW and the content of isoleucine and valine in breast muscles.
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Kopec
authors.affiliation: Department of Animal Nutrition and Feed Quality, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Wiliczkiewicz
authors.affiliation: Department of Animal Nutrition and Feed Quality, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Jamroz
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Biazik
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Pudlo
authors.affiliation: Department of Animal Nutrition and Feed Quality, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Hikawczuk
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Skiba
authors.affiliation: Department of Animal Products Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland malgorzata.korzeniowska@up.wroc.pl.
authors.forename: M
authors.initials: M
authors.lastname: Korzeniowska
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000861:Anserine / Q000097:blood / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D001773:Blood Cells / Q000737:chemistry; D002336:Carnosine / Q000097:blood / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D057141:Food, Preserved / Q000032:analysis; D006639:Histidine / Q000032:analysis / Q000378:metabolism*; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D010084:Oxidation-Reduction; D011897:Random Allocation; D014422:Turkeys / Q000097:blood / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D006639:Histidine; D002336:Carnosine; D000861:Anserine
keywords: antioxidative potential; carnosine; histidine; turkey meat