title: Short Communication: Limited HIV Pretreatment Drug Resistance Among Adults Attending Free Antiretroviral Therapy Clinic of Pune, India.
abstract: In India, the roll out of the free antiretroviral therapy (ART) program completed a decade of its initiation in 2014. The success of first-line ART is influenced by prevalence of HIV pretreatment drug resistance (PDR) in the population. In this cross-sectional study, we sought to determine the prevalence of PDR among adults attending the state-sponsored free ART clinic in Pune in western India. Fifty-two individuals eligible for ART as per national guidelines with median CD4 cell count of 253 cells/mm(3) (inter quartile range: 149-326) were recruited between January 2014 and April 2015. Population-based sequencing of partial pol gene sequences from plasma specimen revealed predominant HIV-1 subtype C infection (96.15%) and presence of single-drug resistance mutations against non-nucleoside reverse transcriptase inhibitor in two sequences. The study supports the need for periodic surveillance, when offering PDR testing at individual level is not feasible.
authors.affiliation: 1 HIV Drug Resistance Laboratory, National AIDS Research Institute , Pune, India .|2 Maharashtra University of Health Sciences , Nashik, India .
authors.forename: Santosh
authors.initials: S
authors.lastname: Karade
authors.affiliation: 1 HIV Drug Resistance Laboratory, National AIDS Research Institute , Pune, India .
authors.forename: Ajit A
authors.initials: AA
authors.lastname: Patil
authors.affiliation: 3 Department of Clinical Sciences, National AIDS Research Institute , Pune, India .
authors.forename: Manisha
authors.initials: M
authors.lastname: Ghate
authors.affiliation: 4 Department of Molecular Virology, National AIDS Research Institute , Pune, India .
authors.forename: Smita S
authors.initials: SS
authors.lastname: Kulkarni
authors.affiliation: 1 HIV Drug Resistance Laboratory, National AIDS Research Institute , Pune, India .
authors.forename: Swarali N
authors.initials: SN
authors.lastname: Kurle
authors.affiliation: 5 Department of Microbiology, National AIDS Research Institute , Pune, India .
authors.forename: Arun R
authors.initials: AR
authors.lastname: Risbud
authors.affiliation: 6 National AIDS Control Organization , New Delhi, India .
authors.forename: Bharat B
authors.initials: BB
authors.lastname: Rewari
authors.affiliation: 3 Department of Clinical Sciences, National AIDS Research Institute , Pune, India .
authors.forename: Raman R
authors.initials: RR
authors.lastname: Gangakhedkar
mesh_terms: D000328:Adult; D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D003430:Cross-Sectional Studies; D024882:Drug Resistance, Viral; D005260:Female; D006678:HIV / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D015658:HIV Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000821:virology*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D020125:Mutation, Missense; D015995:Prevalence; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D044966:Anti-Retroviral Agents; D054302:pol Gene Products, Human Immunodeficiency Virus