title: Genome characterization and phylogenetic analysis of a lineage IV peste des petits ruminants virus in southern China.
abstract: Since 2013, the second outbreak of peste des petits ruminants (PPR) caused by Peste des petits ruminants virus (PPRV) has spread over more than 20 provinces, municipalities, and autonomous regions in China, resulting in major economic losses for livestock industry. In 2014, we encountered a clinical PPR case on a goat farm in Guangdong province, southern China. The complete genome of this PPRV strain, named CH/GDDG/2014, was sequenced to determine its similarities and differences with other strains. The CH/GDDG/2014 genome comprised 15,954 nucleotides (six nucleotides more than classical PPRVs identified before 2013, but complying with the rule of six) with six open reading frames encoding nucleocapsid protein, phosphoprotein, matrix protein, fusion protein, hemagglutinin, and large polymerase protein, respectively. The whole-genome-based alignment analysis indicated that CH/GDDG/2014 had the most proximate consensus (99.8 %) to China/XJYL/2013 and the least consensus (87.2 %) to KN5/2011. The phylogenetic analysis showed that CH/GDDG/2014 was clustered in one branch (lineage IV) with other emerging strains during the second outbreak. This study is the first report describing the whole-genome sequence of PPRV in Guangdong province, southern China and also suggests the PPR outbreak may be closely related to illegal cross-regional importation of goats.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. 616483942@qq.com.
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Li
authors.affiliation: Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Key Labratory of Animal Disease Prevention, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou, 510640, China. zhaishaolun@163.com.
authors.forename: Shao-Lun
authors.initials: SL
authors.lastname: Zhai
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. dhe@scau.edu.cn.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: He
authors.affiliation: Guangdong Key Laboratory of Laboratory Animals, Guangzhou, 510663, China. 1518227522@qq.com.
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Guo
authors.affiliation: Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Key Labratory of Animal Disease Prevention, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou, 510640, China. 137830037@qq.com.
authors.forename: Dian-Hong
authors.initials: DH
authors.lastname: Lv
authors.affiliation: Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Key Labratory of Animal Disease Prevention, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou, 510640, China. 282895173@qq.com.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wen
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. luoml@scau.edu.cn.
authors.forename: Man-Lin
authors.initials: ML
authors.lastname: Luo
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, No. 483 Wushan Road, Tianhe District, Guangzhou, 510642, China. chensa727@126.com.
authors.forename: Rui-Ai
authors.initials: RA
authors.lastname: Chen
authors.affiliation: Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Key Labratory of Animal Disease Prevention, No. 21 Baishigang Street, Wushan Road, Tianhe District, Guangzhou, 510640, China. wwk188@tom.com.
authors.forename: Wen-Kang
authors.initials: WK
authors.lastname: Wei
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D004196:Disease Outbreaks; D005814:Genes, Viral; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D019590:Nucleocapsid Proteins / Q000235:genetics; D029021:Peste-des-Petits-Ruminants / Q000235:genetics* / Q000401:mortality / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D019590:Nucleocapsid Proteins
keywords: Complete genome sequence; Goat; Imported case; Peste des petits ruminants virus; Southern China