title: Antibodies as means for selective mass spectrometry.
abstract: For protein analysis of biological samples, two major strategies are used today; mass spectrometry (MS) and antibody-based methods. Each strategy offers advantages and drawbacks. However, combining the two using an immunoenrichment step with MS analysis brings together the benefits of each method resulting in increased sensitivity, faster analysis and possibility of higher degrees of multiplexing. The immunoenrichment can be performed either on protein or peptide level and quantification standards can be added in order to enable determination of the absolute protein concentration in the sample. The combination of immunoenrichment and MS holds great promise for the future in both proteomics and clinical diagnostics. This review describes different setups of immunoenrichment coupled to mass spectrometry and how these can be utilized in various applications.
authors.affiliation: School of Biotechnology, Division of Protein Technology, KTH-Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Tove
authors.initials: T
authors.lastname: Boström
authors.affiliation: School of Biotechnology, Division of Proteomics and Nanobiotechnology, KTH-Royal Institute ofTechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Jenny Ottosson
authors.initials: JO
authors.lastname: Takanen
authors.affiliation: School of Biotechnology, Division of Protein Technology, KTH-Royal Institute of Technology, AlbaNova University Center, SE-106 91 Stockholm, Sweden. Electronic address: sophia@biotech.kth.se.
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D000906:Antibodies
keywords: Affinity enrichment; Antibody; Human Protein Atlas; Immunoaffinity MS; Mass spectrometry; Protein quantification; Review