title: Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.
abstract: Protein-protein interaction studies provide useful insights into biological processes taking place within the living cell. A number of techniques are available to unravel large structural protein complexes, functional protein modules, and temporary protein associations occurring during signal transduction. The choice of method depends on the nature of the proteins and the interaction being studied. Here we present an optimized and simplified yeast three-hybrid method for analysis of protein interactions involving three components.
authors.affiliation: Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Maruta
authors.affiliation: Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Trusov
authors.affiliation: Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia. j.botella@uq.edu.au.
authors.forename: Jose R
authors.initials: JR
authors.lastname: Botella
mesh_terms: D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins
keywords: Protein complexes; Protein interaction; Signal transduction; Signaling cascades; Yeast three hybrid; Yeast two hybrid