title: Protective effects of ellagic acid and ozone on rat ovaries with an ischemia/reperfusion injury.
abstract: OBJECTIVE
  This study investigated the effects of the antioxidant agents, ozone (O) and ellagic acid (EA), on ischemia/reperfusion (I/R) injuries developed from an ovarian torsion-detorsion model.
  
  
  METHODS
  Arteries in the left ovaries of rats were clamped for two hours to achieve torsion, and then the clamps were removed for a two-hour detorsion period. Thirty-five female Sprague-Dawley rats were randomly divided into five groups: control: administered only with anesthesia, rats were not subjected to torsion-detorsion; I/R: subjected to torsion and subsequent detorsion, without administering any treatment agent; and I/R + EA, I/R + O and I/R + O + EA: subjected to torsion and detorsion processes and administered with EA, O or EA + O at the 75th minute of torsion. The rats were then sacrificed under general anesthesia and the ovarian tissues were excised. The tissues were homogenized and levels of glutathione reductase, catalase, superoxide dismutase and malondialdehyde (MDA) were analyzed. Tissue damage was evaluated in terms of histopathological parameters, such as hemorrhage, congestion, edema and inflammation.
  
  
  RESULTS
  Antioxidant enzyme activity and MDA levels in the ovary tissue increased in the I/R group and decreased in the O, EA and O + EA groups (P < 0.05). Histopathological examination revealed that tissue damage in the O, EA and O + EA groups decreased in comparison with the I/R group (P < 0.05).
  
  
  CONCLUSIONS
  These biochemical and histopathological findings suggest that EA and O are effective against ovarian I/R injury.
authors.affiliation: Department of Pathology, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Ilyas
authors.initials: I
authors.lastname: Sayar
authors.affiliation: Department of Child Surgery, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Senol
authors.initials: S
authors.lastname: Bicer
authors.affiliation: Department of Physiology, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Gursul
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gürbüzel
authors.affiliation: Department of General Surgery, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Kemal
authors.initials: K
authors.lastname: Peker
authors.affiliation: Department of General Surgery, Faculty of Medicine, Erzincan University, Erzincan.
authors.forename: Arda
authors.initials: A
authors.lastname: Işik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D004610:Ellagic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D010049:Ovarian Diseases / Q000188:drug therapy* / Q000378:metabolism; D010053:Ovary / Q000098:blood supply / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014102:Torsion Abnormality / Q000188:drug therapy* / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D020011:Protective Agents; D004610:Ellagic Acid; D008315:Malondialdehyde; D010126:Ozone; D002374:Catalase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase
keywords: ellagic acid; immunohistochemistry; ischemia/reperfusion; ovary; ozone