title: Genetic Polymorphism and Expression of CXCR4 in Breast Cancer.
abstract: CXCR4 genetic polymorphisms, as well as their expression level, have been associated with cancer development and prognosis. The present study aimed to investigate the influence of CXCR4 rs2228014 polymorphism on its mRNA and protein expression in breast cancer samples. It was observed that patients presented higher CXCR4 mRNA relative expression (5.7-fold) than normal mammary gland, but this expression was not correlated with patients clinicopathological features (nuclear grade, nodal status, ER status, PR status, p53 staining, Ki67 index, and HER-2 status). Moreover, CXCR4 mRNA relative expression also did not differ regarding the presence or absence of T allele (p = 0.301). In the immunohistochemical assay, no difference was observed for CXCR4 cytoplasmic protein staining in relation to different genotypes (p = 0.757); however, high cytoplasmic CXCR4 staining was verified in invasive breast carcinoma (p < 0.01). All in all, the results from present study indicated that rs2228014 genetic variant does not alter CXCR4 mRNA or protein expression. However, this receptor was more expressed in tumor compared to normal tissue, in both RNA and protein levels, suggesting its promising applicability in the general context of mammary carcinogenesis.
authors.affiliation: Laboratory of Human Pathology, Department of Pathology, Clinical Analysis and Toxicology, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Okuyama Kishima
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Karen
authors.initials: K
authors.lastname: Brajão de Oliveira
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Carolina Batista
authors.initials: CB
authors.lastname: Ariza
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Carlos Eduardo Coral
authors.initials: CE
authors.lastname: de Oliveira
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Losi Guembarovski
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Bruna Karina
authors.initials: BK
authors.lastname: Banin Hirata
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Felipe Campos
authors.initials: FC
authors.lastname: de Almeida
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Glauco Akelinghton Freire
authors.initials: GA
authors.lastname: Vitiello
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Kleber Paiva
authors.initials: KP
authors.lastname: Trugilo
authors.affiliation: Laboratory of Human Pathology, Department of Pathology, Clinical Analysis and Toxicology, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Alda Fiorina Maria Losi
authors.initials: AF
authors.lastname: Guembarovski
authors.affiliation: Gineco-Med Clinical of Mastology, Londrina, PR, Brazil.
authors.forename: Walter
authors.initials: W
authors.lastname: Jorge Sobrinho
authors.affiliation: Cancer Hospital of Londrina, Londrina, PR, Brazil.
authors.forename: Clodoaldo Zago
authors.initials: CZ
authors.lastname: Campos
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Pr 445 Km 380 Celso Garcia Cid Highway, 86057-970 Londrina, PR, Brazil.
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D019718:Receptors, CXCR4 / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D019718:Receptors, CXCR4