title: Response to Pick.
abstract: In his letter, Dr. Pick criticizes our use of relative values when representing the NOX2 inhibitory action of a novel small molecule (GSK2795039) in a semi-recombinant NOX2 membrane assay. To address this concern, we performed additional experiments using the superoxide inhibitable assays cytochrome C and water soluble tetrazolium salt (WST-1) reduction. In this letter, we document turnover values between 80 and 100 mol O2(•-)/s/mol cytochrome b558 in our semi-recombinant assay and confirmed that GSK2795039 inhibits the NOX2 isoform in the submicromolar range. Antioxid. Redox Signal. 23, 1251-1253.
authors.affiliation: 1 Department of Pathology and Immunology, Geneva Medical Faculty, Geneva University Hospitals , Centre Médical Universitaire, Geneva, Switzerland .
authors.forename: Vincent
authors.initials: V
authors.lastname: Jaquet
authors.affiliation: 2 Neural Pathways Discovery Performance Unit, Neurosciences Therapeutic Area, GlaxoSmithKline, Biopolis , Singapore .
authors.forename: A Richard
authors.initials: AR
authors.lastname: Rutter
mesh_terms: D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D055808:Drug Discovery; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D000631:Aminopyridines; D004791:Enzyme Inhibitors; D008562:Membrane Glycoproteins; D013449:Sulfonamides; D019255:NADPH Oxidases