title: A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column.
abstract: A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB bus-powered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.
authors.affiliation: Division of Applied Chemistry, Faculty of Engineering, Hokkaido University.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishida
authors.forename: Mitsutaka
authors.initials: M
authors.lastname: Fujii
authors.forename: Takehiro
authors.initials: T
authors.lastname: Fujimoto
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Sasaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yanagisawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tani
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi