title: Transcriptomics in cancer diagnostics: developments in technology, clinical research and commercialization.
abstract: Transcriptomic technologies are evolving to diagnose cancer earlier and more accurately to provide greater predictive and prognostic utility to oncologists and patients. Digital techniques such as RNA sequencing are replacing still-imaging techniques to provide more detailed analysis of the transcriptome and aberrant expression that causes oncogenesis, while companion diagnostics are developing to determine the likely effectiveness of targeted treatments. This article examines recent advancements in molecular profiling research and technology as applied to cancer diagnosis, clinical applications and predictions for the future of personalized medicine in oncology.
authors.affiliation: a College of Arts and Sciences , Washington University in St. Louis , St. Louis , MO , USA.|b Rare Genomics Institute , Bethesda , MD , USA.
authors.forename: Monica
authors.initials: M
authors.lastname: Sager
authors.affiliation: b Rare Genomics Institute , Bethesda , MD , USA.|c School of Medicine , Washington University in St. Louis , St. Louis , MO , USA.
authors.forename: Nai Chien
authors.initials: NC
authors.lastname: Yeat
authors.affiliation: a College of Arts and Sciences , Washington University in St. Louis , St. Louis , MO , USA.|b Rare Genomics Institute , Bethesda , MD , USA.
authors.forename: Stefan
authors.initials: S
authors.lastname: Pajaro-Van der Stadt
authors.affiliation: b Rare Genomics Institute , Bethesda , MD , USA.|c School of Medicine , Washington University in St. Louis , St. Louis , MO , USA.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lin
authors.affiliation: b Rare Genomics Institute , Bethesda , MD , USA.|d Whiting School of Engineering , Johns Hopkins University , Baltimore , MD , USA.
authors.forename: Qiuyin
authors.initials: Q
authors.lastname: Ren
authors.affiliation: b Rare Genomics Institute , Bethesda , MD , USA.
authors.forename: Jimmy
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D035843:Biomedical Research; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D057285:Precision Medicine / Q000379:methods; D018581:Technology Transfer; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor
keywords: RNA sequencing; Transcriptomics; cancer; companion diagnostics; microarray; molecular diagnostics; personalized medicine; quantitative reverse transcription PCR (RT-qPCR); serial analysis of gene expression (SAGE)