title: Protective effect of TSLP delivered at the gut mucosa level by recombinant lactic acid bacteria in DSS-induced colitis mouse model.
abstract: BACKGROUND
  Thymic stromal lymphopoietin (TSLP) is a cytokine known to mature dendritics cells, lower pro-inflammatory IL-12 secretion, induce differentiation of anti-inflammatory FoxP3+ regulatory T cells (Treg). Moreover, Crohn's disease patients have shown a reduction of intestinal TSLP expression. To understand the role of TSLP in inflammation, we constructed Lactococcus lactis strain producing TSLP (LL-TSLP) and investigated the effect of its administration on dextran sulfate sodium (DSS)-induced colitis model in mice.
  
  
  RESULTS
  LL-TSLP secrete an active molecule which lowers secretion of IL-12 by dendritic cells. Treatment with LL-TSLP, increases the amount of TGF-β secreted by T cells in Mesenteric Lymph Node in healthy mice. In acute DSS-induced colitis, LL-TSLP delayed the Disease Activity Index and lowered histological score and colonic INF-γ production. In a DSS-recovery model, LL-TSLP induced a better protective effect if the strain was administered at the beginning of the colitis. At Day 4 of colitis we observed an induction of Treg by LL-TSLP.
  
  
  CONCLUSIONS
  TSLP showed an anti-inflammatory protective role in DSS-induced colitis. We have demonstrated that a short and early administration of LL-TSLP is more efficient than a long lasting treatment.
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. aubrycamille@yahoo.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. aubrycamille@yahoo.fr.
authors.forename: Camille
authors.initials: C
authors.lastname: Aubry
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. michon.christophe@yahoo.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. michon.christophe@yahoo.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Michon
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. florian.chain@jouy.inra.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. florian.chain@jouy.inra.fr.
authors.forename: Florian
authors.initials: F
authors.lastname: Chain
authors.affiliation: Merck Serono SA, 1170, Aubonne, Switzerland. yolande.chvatchko@calypsobiotech.com.
authors.forename: Yolande
authors.initials: Y
authors.lastname: Chvatchenko
authors.affiliation: Merck Serono SA, 1170, Aubonne, Switzerland. laurence.goffin@calypsobiotech.com.
authors.forename: Laurence
authors.initials: L
authors.lastname: Goffin
authors.affiliation: EMD Serono, 45A Middlesex Turnpike, Billerica, MA, 01821, USA. simone.favre-zimmerli@emdserono.com.
authors.forename: Simone C
authors.initials: SC
authors.lastname: Zimmerli
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. sylvia.leguin@jouy.inra.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. sylvia.leguin@jouy.inra.fr.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Leguin
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. philippe.langella@jouy.inra.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. philippe.langella@jouy.inra.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. luis.bermudez@jouy.inra.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. luis.bermudez@jouy.inra.fr.
authors.forename: Luis
authors.initials: L
authors.lastname: Bermudez-Humaran
authors.affiliation: INRA, UMR1319 Micalis, 78350, Jouy-en-Josas, France. jean-marc.chatel@jouy.inra.fr.|AgroParisTech, UMR Micalis, 78350, Jouy-en-Josas, France. jean-marc.chatel@jouy.inra.fr.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chatel
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D003106:Colon / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism; D016264:Dextran Sulfate / Q000633:toxicity; D004195:Disease Models, Animal; D007249:Inflammation / Q000517:prevention & control; D018664:Interleukin-12 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000382:microbiology; D013294:Lactococcus lactis / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D016212:Transforming Growth Factor beta; D018664:Interleukin-12; D016264:Dextran Sulfate; D000094632:Thymic Stromal Lymphopoietin