title: Molecular Cloning, Expression Pattern and Genotypic Effects on Glucoraphanin Biosynthetic Related Genes in Chinese Kale (Brassica oleracea var. alboglabra Bailey).
abstract: Glucoraphanin is a plant secondary metabolite that is involved in plant defense and imparts health-promoting properties to cruciferous vegetables. In this study, three genes involved in glucoraphanin metabolism, branched-chain aminotransferase 4 (BCAT4), methylthioalkylmalate synthase 1 (MAM1) and dihomomethionine N-hydroxylase (CYP79F1), were cloned from Chinese kale (Brassica oleracea var. alboglabra Bailey). Sequence homology and phylogenetic analysis identified these genes and confirmed the evolutionary status of Chinese kale. The transcript levels of BCAT4, MAM1 and CYP79F1 were higher in cotyledon, leaf and stem compared with flower and silique. BCAT4, MAM1 and CYP79F1 were expressed throughout leaf development with lower transcript levels during the younger stages. Glucoraphanin content varied extensively among different varieties, which ranged from 0.25 to 2.73 µmol·g(-1) DW (dry weight). Expression levels of BCAT4 and MAM1 were high at vegetative-reproductive transition phase, while CYP79F1 was expressed high at reproductive phase. BCAT4, MAM1 and CYP79F1 were expressed significantly high in genotypes with high glucoraphanin content. All the results provided a better understanding of the roles of BCAT4, MAM1 and CYP79F1 in the glucoraphanin biosynthesis of Chinese kale.
authors.affiliation: Department of Hortscience, South China Agricultural University, Guangzhou 510642, China. 1987.yinling@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Hortscience, South China Agricultural University, Guangzhou 510642, China. cmchen@scau.edu.cn.
authors.forename: Changming
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Hortscience, South China Agricultural University, Guangzhou 510642, China. gjchen@scau.edu.cn.
authors.forename: Guoju
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Hortscience, South China Agricultural University, Guangzhou 510642, China. caobh01@scau.edu.cn.
authors.forename: Bihao
authors.initials: B
authors.lastname: Cao
authors.affiliation: Department of Hortscience, South China Agricultural University, Guangzhou 510642, China. jjlei@scau.edu.cn.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Lei
mesh_terms: D001937:Brassica / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D005961:Glucosinolates / Q000096:biosynthesis*; D007096:Imidoesters; D010091:Oximes; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013454:Sulfoxides; D000637:Transaminases / Q000235:genetics / Q000378:metabolism
chemical_list: D005961:Glucosinolates; D007096:Imidoesters; D010091:Oximes; D010940:Plant Proteins; D013454:Sulfoxides; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase; C119494:glucoraphanin
keywords: BCAT4; CYP79F1; Chinese kale; MAM1; glucoraphanin