title: Phosphatase activity of Bombyx mori nucleopolyhedrovirus PTP is dispensable for enhanced locomotory activity in B. mori larvae.
abstract: Baculovirus-induced enhanced locomotory activity (ELA) is not induced in caterpillars infected with a mutant Bombyx mori nucleopolyhedrovirus (BmNPV) or Autographa californica multiple nucleopolyhedrovirus (AcMNPV) lacking a functional protein tyrosine phosphatase gene (ptp). Previous studies suggest that the PTP proteins from BmNPV and AcMNPV act in different ways to induce ELA, i.e., BmNPV PTP is utilized as a virion structural component, whereas AcMNPV PTP requires its phosphatase activity. Here, I generated and characterized two new BmNPV mutants expressing enzymatically inactive PTP proteins and confirmed that the phosphatase activity of PTP is not required for ELA induction in BmNPV-infected B. mori larvae.
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: katsuma@ss.ab.a.u-tokyo.ac.jp.
authors.forename: Susumu
authors.initials: S
authors.lastname: Katsuma
mesh_terms: D000818:Animals; D012831:Bombyx / Q000502:physiology / Q000821:virology*; D054884:Host-Pathogen Interactions; D007814:Larva / Q000502:physiology / Q000821:virology; D009043:Motor Activity; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D017027:Protein Tyrosine Phosphatases
keywords: Baculovirus; BmNPV; ELA; PTP