title: [Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene].
abstract: β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.
authors.forename: Yi-mei
authors.initials: YM
authors.lastname: Zang
authors.forename: Yan-peng
authors.initials: YP
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Qiao
authors.forename: Hong-hao
authors.initials: HH
authors.lastname: Chen
authors.forename: Chun-sheng
authors.initials: CS
authors.lastname: Liu
mesh_terms: D005091:Exons; D005838:Genotype; D029882:Glycyrrhiza uralensis / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D019751:Intramolecular Transferases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D010940:Plant Proteins; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase