title: Synchronized growth and neutral lipid accumulation in Chlorella sorokiniana FC6 IITG under continuous mode of operation.
abstract: Synchronized growth and neutral lipid accumulation with high lipid productivity under mixotrophic growth of the strain Chlorella sorokiniana FC6 IITG was achieved via manipulation of substrates feeding mode and supplementation of lipid elicitors in the growth medium. Screening and optimization of lipid elicitors resulted in lipid productivity of 110.59mgL(-1)day(-1) under the combined effect of lipid inducers sodium acetate and sodium chloride. Fed-batch cultivation of the strain in bioreactor with intermittent feeding of limiting nutrients and lipid inducer resulted in maximum biomass and lipid productivity of 2.08 and 0.97gL(-1)day(-1) respectively. Further, continuous production of biomass with concomitant lipid accumulation was demonstrated via continuous feeding of BG11 media supplemented with lipid inducers sodium acetate and sodium chloride. The improved biomass and lipid productivity in chemostat was found to be 2.81 and 1.27gL(-1)day(-1) respectively operated at a dilution rate of 0.54day(-1).
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Vikram
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Muthusivaramapandian
authors.initials: M
authors.lastname: Muthuraj
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
authors.forename: Basavaraj
authors.initials: B
authors.lastname: Palabhanvi
authors.affiliation: Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India; Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India. Electronic address: debasishd@iitg.ernet.in.
authors.forename: Debasish
authors.initials: D
authors.lastname: Das
mesh_terms: D000704:Analysis of Variance; D061249:Batch Cell Culture Techniques; D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology; D019346:Sodium Acetate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D012965:Sodium Chloride; D019346:Sodium Acetate
keywords: Chemostat; Chlorella; Lipid inducers; Mixotrophic; Simultaneous growth and lipid