title: Impact of the novel neonicotinoid insecticide Paichongding on bacterial communities in yellow loam and Huangshi soils.
abstract: Insecticides are widely sprayed in modern agriculture for ensuring the crop yield, which could also lead to contamination and insecticide residue in soils. Paichongding (IPP) is a novel neonicotinoid insecticide and was developed recently in China. Soil bacterial community, diversity, and community composition vary widely depending on environmental factors. As for now, little is known about bacterial species thriving, bacterial community diversity, and structure in IPP-spraying soils. In present study, IPP degradation in yellow loam and Huangshi soils was investigated, and bacterial communities and diversity were examined in soil without IPP spray and with IPP spray through pyrosequencing of 16S ribosomal RNA (rRNA) gene amplicons. The degradation ratio of IPP at 60 days after treatment (DAT) reached 51.22 and 34.01 % in yellow loam and Huangshi soil, respectively. A higher richness of operational taxonomic units (OTUs) was found in yellow loam soil (867 OTUs) and Huangshi soil (762 OTUs) without IPP spray while OUTs were relatively low in IPP-spraying soils. The community composition also differed both in phyla and genus level between these two environmental conditions. Proteobacteria, Firmicutes, Planctomycetes, Chloroflexi, Armatimonadetes, and Chlorobi were stimulated to increase after IPP application, while IPP inhibited the phyla of Bacteroidetes, Actinobacteria, and Acidobacteria.
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China. zhqcai@cczu.edu.cn.
authors.forename: Zhiqiang
authors.identifier: http://orcid.org/0000-0002-9180-675X
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Ma
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Cai
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Yang
authors.affiliation: Laboratory of Applied Microbiology, School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, 213164, China. xyzhao@cczu.edu.cn.
authors.forename: Xiyue
authors.initials: X
authors.lastname: Zhao
mesh_terms: D061271:Acidobacteria / Q000187:drug effects; D039903:Actinobacteria / Q000187:drug effects; D053961:Azabicyclo Compounds / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics; D002681:China; D007306:Insecticides / Q000494:pharmacology*; D020560:Proteobacteria / Q000235:genetics; D011725:Pyridines / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil; D012988:Soil Microbiology
chemical_list: D053961:Azabicyclo Compounds; D007306:Insecticides; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; D012987:Soil; C588015:paichongding
keywords: Bacterial community; Bacterial diversity; Degradation; Paichongding; Pyrosequencing