title: HSP70 overexpression may play a protective role in the mouse embryos stimulated by CUMS.
abstract: BACKGROUND
  We evaluated whether heat shock protein HSP70 plays a protective role in the embryos of Kunming mice subjected to chronic unpredictable mild stress.
  
  
  METHODS
  Female mice were stimulated for 4 weeks with nine stressors and then divided into mild, moderate and severe stress groups. Superovulation was induced with a gonadotropin preparation (PMSG/HCG) and HSP70 expression in 2-cell embryos and day 4 embryos was detected by immunofluorescence (IF) and real-time polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  In the mild stress group, ovarian response and oocyte development potential were similar to those of the control group, while the HSP70 mRNA levels of the embryos were significantly higher (P < 0.05). In the severe stress group, ovarian response and oocyte development potential decreased compared with the control group (P < 0.05), while the HSP70 mRNA levels were similar. The results of the moderate stress group were intermediate among the three groups. Furthermore, HSP70 mRNA levels of the embryos were shown to be positively associated with parameters of oocyte and embryo development potential (P < 0.05).
  
  
  CONCLUSIONS
  HSP70 overexpression may play a protective role in the embryos of the mild or moderate stress mice stimulated by chronic unpredictable mild stress.
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. lixiaohongham_001@163.com.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.affiliation: Department of Ultrasonography, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. 785365610@qq.com.
authors.forename: Hou-Qing
authors.initials: HQ
authors.lastname: Pang
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. qinglang123@163.com.
authors.forename: Lang
authors.initials: L
authors.lastname: Qin
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. js1972@163.com.
authors.forename: Song
authors.initials: S
authors.lastname: Jin
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. zengxunxun@126.com.
authors.forename: Xun
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. byfxfey@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Reproductive Medicine Center, West China Second Hospital of Sichuan University, Chengdu, Sichuan, China. lishangweilxh_001@163.com.
authors.forename: Shang-Wei
authors.initials: SW
authors.lastname: Li
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D013312:Stress, Physiological; D013315:Stress, Psychological / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins