title: BSA-conjugated zinc oxide nanoparticles as luminescent probes for the determination of histidine.
abstract: Zinc oxide nanoparticles doped with bovine serum albumin were used to determine histidine in aqueous solutions using a fluorescence spectroscopic technique. The results showed that histidine effectively quenched the fluorescence of the modified ZnO nanoparticles, whereas other amino acids did not significantly affect the light emission, thereby allowing selective and sensitive histidine detection in amino acid mixtures. Under optimal conditions (pH 7.0, 25 °C, 10 min preincubation), the detection limit for histidine was ~ 9.87 × 10(-7) mol/L. The high value of the determined quenching rate constant Kq (3.30 × 10(13)  L/mol/s) was consistent with a static quenching mechanism. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: School of Pharmacy, Guangdong Medical University, Dongguan, 523808, People's Republic of China.
authors.forename: Dudu
authors.initials: D
authors.lastname: Wu
authors.affiliation: School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China.
authors.forename: Qiaowen
authors.initials: Q
authors.lastname: Liang
authors.affiliation: School of Pharmacy, Guangdong Medical University, Dongguan, 523808, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D006639:Histidine / Q000032:analysis*; D049408:Luminescent Agents / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D012710:Serum Albumin, Bovine; D006639:Histidine; D015034:Zinc Oxide
keywords: fluorescence intensity; histidine; nanoparticles; zinc oxide