title: LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization during Dermal Regeneration.
abstract: High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.
authors.affiliation: Department of Plastic Surgery and Hand Surgery, University of Lübeck, Lübeck, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Kisch
authors.affiliation: Fraunhofer Research Institution for Marine Biotechnology, Lübeck, Germany.
authors.forename: Caroline
authors.initials: C
authors.lastname: Weber
authors.affiliation: Fraunhofer Research Institution for Marine Biotechnology, Lübeck, Germany.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Rapoport
authors.affiliation: Fraunhofer Research Institution for Marine Biotechnology, Lübeck, Germany.
authors.forename: Charli
authors.initials: C
authors.lastname: Kruse
authors.affiliation: Fraunhofer Research Institution for Marine Biotechnology, Lübeck, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Schumann
authors.affiliation: Department of Plastic Surgery and Hand Surgery, University of Lübeck, Lübeck, Germany.
authors.forename: Felix H
authors.initials: FH
authors.lastname: Stang
authors.affiliation: Department of Plastic Surgery and Hand Surgery, BG-Kliniken Bergmannstrost, Halle, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Siemers
authors.affiliation: Fraunhofer Research Institution for Marine Biotechnology, Lübeck, Germany.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Matthießen
mesh_terms: D000818:Animals; D001706:Biopsy; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000737:chemistry; D006801:Humans; D007249:Inflammation; D008070:Lipopolysaccharides / Q000737:chemistry; D051379:Mice; D008819:Mice, Nude; D018919:Neovascularization, Physiologic; D012038:Regeneration / Q000502:physiology*; D012867:Skin / Q000098:blood supply* / Q000166:cytology / Q000473:pathology*; D012879:Skin Physiological Phenomena; D017768:Skin, Artificial; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014945:Wound Healing
chemical_list: D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D003094:Collagen