title: [Analysis on HIV-1 genetics and threshold of drug resistance in Dehong prefecture of Yunnan province in 2013].
abstract: OBJECTIVE
  To study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2013.
  
  
  METHODS
  Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 54 plasma samples of recently reported HIV-infected individuals, aged between 16 and 25 years, were collected in Dehong prefecture from January to August 2013. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype. Prevalent levels of HIV-1 drug resistance transmission were analyzed.
  
  
  RESULTS
  Forty-eight plasma samples were successfully sequenced and analyzed. Among them, 45.8% were Chinese and the rest 54.2% were all Burmese. Based on pol sequences, identified HIV genotypes included subtype C (41.7%), URF (31.3%), CRF01_AE (12.5%), CRF07_BC (10.4%), CRF08_BC (2.1%) and subtype B (2.1%), C subtype appeared dominated in Chinese while URF was dominated in Burmese. One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in one sequence from Burmese. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as < 5%.
  
  
  CONCLUSIONS
  Diverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as at low prevalence level, in Dehong. To prevent the increase of the prevalence of transmitted HIV-1 drug resistance, standard treatment and scientific management for people living with HIV/AIDS should be strictly followed. Meanwhile, relevant surveillance, including drug resistance surveillance should also be performed among cross-border migrant population.
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Dehong Center for Disease Control and Prevention.
authors.forename: Jibao
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention.
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Dehong Center for Disease Control and Prevention.
authors.forename: Shitang
authors.initials: S
authors.lastname: Yao
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Huichao
authors.initials: H
authors.lastname: Chen
authors.affiliation: Dehong Center for Disease Control and Prevention.
authors.forename: Jin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Dehong Center for Disease Control and Prevention.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Li
authors.affiliation: Dehong Center for Disease Control and Prevention; Email: dhduansong@sina.com.
authors.forename: Song
authors.initials: S
authors.lastname: Duan
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China; Email: jiamanhong@hotmail.com.
authors.forename: Manhong
authors.initials: M
authors.lastname: Jia
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology / Q000627:therapeutic use; D002681:China; D024882:Drug Resistance, Viral / Q000235:genetics*; D015751:Genes, pol; D005838:Genotype; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D015995:Prevalence; D018894:Reverse Transcriptase Inhibitors
chemical_list: D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors