title: Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity.
abstract: Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Somi
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: TaeHyun
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Hye-Ryeon
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Young-Yun
authors.initials: YY
authors.lastname: Kong
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
keywords: Hippocampus; Memory; Mind bomb-2; Notch signaling; Synaptic plasticity