title: Facile in Situ Synthesis of Silver Nanoparticles on the Surface of Metal-Organic Framework for Ultrasensitive Surface-Enhanced Raman Scattering Detection of Dopamine.
abstract: Surface-enhanced Raman scattering (SERS) signals are intensively dominated by the Raman hot spots and distance between analyte molecules and metallic nanostructures. Herein, an efficient SERS substrate was developed by in situ synthesis of silver nanoparticles (AgNPs) on the surface of MIL-101 (Fe), a typical metal-organic framework (MOF). The as-prepared SERS substrate combines the numerous Raman hot spots between the high-density Ag NPs and the excellent adsorption performance of MOFs, making it an excellent SERS substrate for highly sensitive SERS detection by effectively concentrating analytes in close proximity to the Raman hot spots domains between the adjacent AgNPs. The resulting hybrid material was used for ultrasensitive SERS detection of dopamine based on the peroxidase-like activity of MIL-101 (Fe) by utilizing the enzyme-linked immunosorbent assay (ELISA) colorimetric substrate, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) as a SERS marker. This new developed method showed good linearity in the range from 1.054 pM to 210.8 nM for dopamine with the correlation coefficient of 0.992, detection limit of approximately 0.32 pM [signal-to-noise ratio (S/N) = 3], and acceptable recoveries ranging from 99.8% to 108.0% in human urine. These results predict that the proposed SERS system may open up a new opportunity for chemical and biological assay applications.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Beibei, Chongqing 400715, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis, Chongqing Science and Technology Commission, College of Pharmaceutical Sciences, Southwest University , Beibei, Chongqing 400716, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Gao
authors.affiliation: Chongqing Key Laboratory of Biomedical Analysis, Chongqing Science and Technology Commission, College of Pharmaceutical Sciences, Southwest University , Beibei, Chongqing 400716, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Beibei, Chongqing 400715, China.|Chongqing Key Laboratory of Biomedical Analysis, Chongqing Science and Technology Commission, College of Pharmaceutical Sciences, Southwest University , Beibei, Chongqing 400716, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University , Beibei, Chongqing 400715, China.
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Li
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D004298:Dopamine / Q000032:analysis*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013059:Spectrum Analysis, Raman; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties
chemical_list: D052160:Benzothiazoles; D009942:Organometallic Compounds; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D012834:Silver; D004298:Dopamine