title: Intravital imaging of the cellular dynamics of LysM-positive cells in a murine corneal suture model.
abstract: BACKGROUND
  Corneal suturing is a surgical procedure used in patients with corneal trauma or transplants. It was reported that endogenous neutrophils are brightly labelled in gene-targeted mice expressing enhanced green fluorescent protein (eGFP) under the control of the endogenous lysozyme M promoter (LysM-eGFP mice).
  
  
  METHODS
  We applied intravital imaging methods to analyse in vivo the dynamics of LysM-positive granulocytes (neutrophils) in LysM-eGFP mice with corneal sutures and examined their role in the elicitation of neutrophil infiltration.
  
  
  RESULTS
  We found that in the presuturing state, neutrophils strongly positive for LysM were located in the periphery of the corneal stromal layer; none were present in the centre of the cornea. After introducing a corneal suture, neutrophils accumulated in limbal vessels and then migrated to the corneal side and the conjunctival side, suggesting that they derived from limbal vessels. Thereafter they accumulated towards the central corneal area, arriving at the suture about 7 h after its placement. Although corneal sutures may elicit the continuous infiltration of neutrophils, their number was markedly decreased by day 1 after suture removal and continued to decrease thereafter.
  
  
  CONCLUSIONS
  Our results showed that corneal sutures may elicit the continuous infiltration of neutrophils.
authors.affiliation: Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ueta
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Koga
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Bioscience, Osaka University, Osaka, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Kikuta
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Ophthalmology, Faculty of Life Science, Kumamoto University, Kumamoto, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Shinomiya
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Bioscience, Osaka University, Osaka, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ishii
authors.affiliation: Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D002465:Cell Movement; D003315:Cornea / Q000601:surgery*; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007634:Keratitis / Q000201:enzymology / Q000209:etiology / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton; D009113:Muramidase / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology / Q000473:pathology*; D013537:Sutures
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D009113:Muramidase; C472450:lysozyme M, mouse
keywords: Cornea; Imaging; Inflammation