title: Biochemical Monitoring of Spinal Cord Injury by FT-IR Spectroscopy--Effects of Therapeutic Alginate Implant in Rat Models.
abstract: Spinal cord injury (SCI) induces complex biochemical changes, which result in inhibition of nervous tissue regeneration abilities. In this study, Fourier-transform infrared (FT-IR) spectroscopy was applied to assess the outcomes of implants made of a novel type of non-functionalized soft calcium alginate hydrogel in a rat model of spinal cord hemisection (n = 28). Using FT-IR spectroscopic imaging, we evaluated the stability of the implants and the effects on morphology and biochemistry of the injured tissue one and six months after injury. A semi-quantitative evaluation of the distribution of lipids and collagen showed that alginate significantly reduced injury-induced demyelination of the contralateral white matter and fibrotic scarring in the chronic state after SCI. The spectral information enabled to detect and localize the alginate hydrogel at the lesion site and proved its long-term persistence in vivo. These findings demonstrate a positive impact of alginate hydrogel on recovery after SCI and prove FT-IR spectroscopic imaging as alternative method to evaluate and optimize future SCI repair strategies.
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden, Germany.|Faculty of Physics, dept. of General Physics and Spectroscopy, Vilnius University, Vilnius, Lithuania.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tamosaityte
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Roberta
authors.initials: R
authors.lastname: Galli
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.
authors.forename: Ortrud
authors.initials: O
authors.lastname: Uckermann
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.
authors.forename: Kerim H
authors.initials: KH
authors.lastname: Sitoci-Ficici
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Later
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, TU Dresden, Dresden, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Beiermeister
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, TU Dresden, Dresden, Germany.
authors.forename: Falko
authors.initials: F
authors.lastname: Doberenz
authors.affiliation: Centre for Translational Bone, Joint and Soft Tissue Research, TU Dresden, Dresden, Germany.|CRTD / DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, TU Dresden, Dresden, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Gelinsky
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Leipnitz
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Schackert
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden, Germany.
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
authors.affiliation: Faculty of Physics, dept. of General Physics and Spectroscopy, Vilnius University, Vilnius, Lithuania.
authors.forename: Valdas
authors.initials: V
authors.lastname: Sablinskas
authors.affiliation: Clinical Sensoring and Monitoring, Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, TU Dresden, Dresden, Germany.|Faculty of Physics, dept. of General Physics and Spectroscopy, Vilnius University, Vilnius, Lithuania.
authors.forename: Gerald
authors.initials: G
authors.lastname: Steiner
authors.affiliation: Neurosurgery, Carl Gustav Carus University Hospital, TU Dresden, Dresden, Germany.|CRTD / DFG-Center for Regenerative Therapies Dresden-Cluster of Excellence, TU Dresden, Dresden, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Kirsch
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D002118:Calcium / Q000737:chemistry; D004195:Disease Models, Animal; D004343:Drug Implants; D005260:Female; D005355:Fibrosis / Q000473:pathology / Q000503:physiopathology; D020723:Glucuronic Acid / Q000737:chemistry / Q000627:therapeutic use; D006603:Hexuronic Acids / Q000737:chemistry / Q000627:therapeutic use; D020100:Hydrogels / Q000737:chemistry*; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared; D013116:Spinal Cord / Q000473:pathology*; D013119:Spinal Cord Injuries / Q000188:drug therapy / Q000503:physiopathology*; D013395:Sucrose / Q000737:chemistry; D014783:Viscosity; D066127:White Matter / Q000503:physiopathology
chemical_list: D000464:Alginates; D004343:Drug Implants; D006603:Hexuronic Acids; D020100:Hydrogels; D013395:Sucrose; D020723:Glucuronic Acid; D002118:Calcium