title: Aneuploidy causes premature differentiation of neural and intestinal stem cells.
abstract: Aneuploidy is associated with a variety of diseases such as cancer and microcephaly. Although many studies have addressed the consequences of a non-euploid genome in cells, little is known about their overall consequences in tissue and organism development. Here we use two different mutant conditions to address the consequences of aneuploidy during tissue development and homeostasis in Drosophila. We show that aneuploidy causes brain size reduction due to a decrease in the number of proliferative neural stem cells (NSCs), but not through apoptosis. Instead, aneuploid NSCs present an extended G1 phase, which leads to cell cycle exit and premature differentiation. Moreover, we show that this response to aneuploidy is also present in adult intestinal stem cells but not in the wing disc. Our work highlights a neural and intestine stem cell-specific response to aneuploidy, which prevents their proliferation and expansion.
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 12 rue Lhomond, Paris 75005, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Gogendeau
authors.affiliation: Institut Curie, CNRS UMR3215, INSERM U934, Paris 75005, France.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Siudeja
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 12 rue Lhomond, Paris 75005, France.
authors.forename: Davide
authors.initials: D
authors.lastname: Gambarotto
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 12 rue Lhomond, Paris 75005, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Pennetier
authors.affiliation: Institut Curie, CNRS UMR3215, INSERM U934, Paris 75005, France.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Bardin
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, 12 rue Lhomond, Paris 75005, France.
authors.forename: Renata
authors.initials: R
authors.lastname: Basto
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D004330:Drosophila; D016193:G1 Phase / Q000235:genetics*; D007150:Immunohistochemistry; D007422:Intestines / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009929:Organ Size; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014921:Wings, Animal / Q000166:cytology