title: [Validation of an in-house method for the determination of zinc in serum: Meeting the requirements of ISO 17025].
abstract: OBJECTIVE
  The aim of this report is to propose a scheme for validation of an analytical technique according to ISO 17025.
  
  
  METHODS
  According to ISO 17025, the fundamental parameters tested were: selectivity, calibration model, precision, accuracy, uncertainty of measurement, and analytical interference.
  
  
  RESULTS
  A protocol has been developed that has been applied successfully to quantify zinc in serum by atomic absorption spectrometry.
  
  
  CONCLUSIONS
  It is demonstrated that our method is selective, linear, accurate, and precise, making it suitable for use in routine diagnostics.
authors.affiliation: Instituto de Toxicología de la Defensa, Madrid, España. Electronic address: maitellorente2000@hotmail.com.
authors.forename: M T
authors.initials: MT
authors.lastname: Llorente Ballesteros
authors.affiliation: Instituto de Toxicología de la Defensa, Madrid, España.
authors.forename: I
authors.initials: I
authors.lastname: Navarro Serrano
authors.affiliation: Instituto de Toxicología de la Defensa, Madrid, España.
authors.forename: J L
authors.initials: JL
authors.lastname: López Colón
mesh_terms: D000068:Accreditation / Q000592:standards; D001774:Blood Chemical Analysis / Q000592:standards*; D002138:Calibration; D006801:Humans; D007390:International Agencies / Q000592:standards; D015588:Observer Variation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D015032:Zinc / Q000097:blood*
chemical_list: D015032:Zinc
keywords: Accreditation; Acreditación; Cinc sérico; ISO 17025; Serum zinc; Validación; Validation