title: [Establishment of a SCID beige mouse model bearing transplanted human choriocarcinoma using JAR cell line].
abstract: OBJECTIVE
  To establish a mouse model bearing human choriocarcinoma xenograft in severe combined immuno-deficient (SCID) beige mice and investigate the disease course and biological behaviors of the tumors.
  
  
  METHODS
  Human choriocarcinoma JAR cells were injected in female SCID beige mice (3-5 weeks old) either subcutaneously (group A, n=6) or via the tail vein (group B, n=6). Morphological studies, radioactive immunoassay, in vivo tumor imaging and histopathological examinations were performed to confirm JAR cell engraftment at the subcutaneous injection site and in the lungs of the mice.
  
  
  RESULTS
  On day 28 after tumor cell inoculation, the mice in group A showed palpable subcutaneous nodules, and HE staining revealed morphological features of the nodules consistent with choriocarcinoma cells; in vivo imaging in group B showed single or multiple solid tumor masses in the lungs, and tissue biopsy examination demonstrated varying degrees of tumor cell infiltration. Compared with the control mice, peripheral blood β-HCG levels in both groups A and B increased significantly on day 14 after cell inoculation (P<0.05), and the increment was more conspicuous in group B (P<0.05).
  
  
  CONCLUSIONS
  Mouse models bearing human choriocarcinoma xenograft can be successfully established by injecting JAR cells either subcutaneously or via the tail vein to mimic the characteristics of epithelial solid tumors and lung metastasis of human choriocarcinoma.
authors.affiliation: Department of Gynecology and Obstetrics, First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. E-mail: tianquan2012@yeah.net.
authors.forename: Quan
authors.initials: Q
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Rong
authors.initials: R
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Rui-Fang
authors.initials: RF
authors.lastname: An
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002822:Choriocarcinoma; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014594:Uterine Neoplasms