title: Steroidal saponins from stems and leaves of Paris polyphylla var. yunnanensis.
abstract: Phytochemical investigation of the stems and leaves of Paris polyphylla var. yunnanensis led to isolation of 12 steroidal saponins, chonglouosides SL-9-SL-20, which had not been described previously, along with 13 known compounds. Their structures were established on the basis of extensive spectroscopic analysis and chemical methods. Four of the twelve steroidal saponins possessed three steroidal aglycones which have not been reported in nature. Steroidal saponins were also evaluated for their cytotoxicities against two human cancer cell lines (HepG2 and HEK293) and anti-HCV effects. One known steroidal saponin was the most cytotoxic compound overall with IC50 values of 2.9 ± 0.5 μM and 5.0 ± 0.6 μM against HepG2 and HEK293 cell lines, respectively, while none showed anti-HCV activity at a concentration of 20 μM.
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Science, Beijing 100049, China.
authors.forename: Xu-Jie
authors.initials: XJ
authors.lastname: Qin
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Science, Beijing 100049, China.
authors.forename: Mu-Yuan
authors.initials: MY
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
authors.forename: Chang-Xiang
authors.initials: CX
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, School of Medicine, Yale University, New Haven, CT, USA.
authors.forename: Yung-Chi
authors.initials: YC
authors.lastname: Cheng
authors.affiliation: Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: haiyangliu@mail.kib.ac.cn.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Liu
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D057809:HEK293 Cells; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000187:drug effects; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D012503:Saponins; D013256:Steroids
keywords: Anti-HCV activity; Chonglouosides SL-9–SL-20; Cytotoxicity; Paris polyphylla var. yunnanensis; Steroidal saponins; Trilliaceae