title: SATB1 is a potential therapeutic target in intrahepatic cholangiocarcinoma.
abstract: BACKGROUND
  Intrahepatic cholangiocarcinoma (ICC) is the second most common primary malignant tumor of the liver with a poor prognosis. Upregulation of special AT-rich sequence-binding protein 1 (SATB1) promotes tumor progression. However, little is known about the role of SATB1 in ICC tumorigenesis.
  
  
  METHODS
  We firstly investigated the expression of SATB1 in 88 cases of ICC by immunohistochemistry (IHC), QRT-PCR, and western blot. Meanwhile, we constructed stably knockdown (shRNA) of SATB1 in ICC cell lines to evaluate the effects of SATB1 on the ability of cell proliferation and invasion by MTT and transwell invasion assay.
  
  
  RESULTS
  Our result showed that SATB1 was overexpressed in ICC tissues samples. Knockdown of SATB1 could inhibit ICC cell proliferation, and suppress ICC cell invasion of ICC cell lines. In addition, the depletion of SATB1 expression suppressed the MYC levels in vitro.
  
  
  CONCLUSIONS
  Our results highlight the significance of SATB1 in ICC and suggest that SATB1 could be a promising therapy target and a potential biomarker for prognosis in ICC patients.
authors.affiliation: Pancreato-Biliary Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Thoracic Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.affiliation: Colorectal Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: K
authors.initials: K
authors.lastname: Wu
authors.affiliation: Pancreato-Biliary Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.affiliation: Pancreato-Biliary Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.affiliation: Pancreato-Biliary Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Hu
authors.affiliation: Pancreato-Biliary Surgery Department, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong, People's Republic of China. drzhangks@163.com.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
mesh_terms: D001650:Bile Duct Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D049109:Cell Proliferation / Q000235:genetics; D018281:Cholangiocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human
keywords: ICC; Invasion; MYC; Proliferation; SATB1; Targeted therapy