title: Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells.
abstract: Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1β) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1β treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium.
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS). University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, España.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gómez-Florit
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS). University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, España.
authors.forename: Marta
authors.initials: M
authors.lastname: Monjo
authors.affiliation: Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS). University of Balearic Islands, Palma de Mallorca, Spain.|Instituto de Investigación Sanitaria de Palma, 07010 Palma, España.
authors.forename: Joana M
authors.initials: JM
authors.lastname: Ramis
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005881:Gingiva / Q000166:cytology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D010519:Periodontium / Q000502:physiology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D055815:Young Adult
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; C012526:quercitrin; D011794:Quercetin