title: [Study on effect of total matrines and extracts from Periplaneta americana on negative endometrial cancer cell JEC of progesterone receptors].
abstract: To study the effect of total matrines and extracts from Periplaneta americana on negative endometrial cancer cell JEC of progesterone receptors. After detecting the effect of total matrine, extracts from P. americana and their combination on JEC cells' growth inhibition, cell cycle, P53 and c-erbB-2 gene protein expressions through MTT, flow cytometry instrument and Western blot method, the author found that, (1) MTT: total matrines and extracts from P. americana could inhibit the growth of JEC cell, with significant increase in the inhibitory effect in the combination group. (2) Flow cytometry instrument: the cell cycle at G0/G1 increased after the treatment with total matrines, the cell cycle at G2/M increased after the treatment with extracts from periplaneta americana, and the ratio of G0/G1 cell cycle in the combination group was significantly higher than the other groups, with inhibition in cell growth and statistical difference in inter-group comparison (P < 0.05). (3) Western blot: the expression level of P53 increased and c-erbB-2 decreased after the treatment with total matrines, extracts from P. americana and their combination on JEC cell, with statistical difference in inter-group comparison (P < 0.05). The above results suggested that total matrines, extracts from P. americana and their combination could induce cell cycle arrest and inhibit the growth of JEC cell by up-regulating P53 and down-regulating the c-erbB-2 level.
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010526:Periplaneta; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D011807:Quinolizines / Q000627:therapeutic use*; D011980:Receptors, Progesterone / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000502:physiology; D000093842:Matrines
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D011807:Quinolizines; D011980:Receptors, Progesterone; D016159:Tumor Suppressor Protein p53; D000093842:Matrines