title: Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C.
abstract: Capillary electrophoresis-based SELEX (CE-SELEX) is an efficient technique for the isolation of aptamers binding to a wide range of target molecules. CE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be performed on non-immobilized targets, usually within a fewer number of selection cycles. Here we describe a complete procedure of CE-SELEX using activated protein C (APC) as the target protein.
authors.affiliation: Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany.
authors.forename: Nasim Shahidi
authors.initials: NS
authors.lastname: Hamedani
authors.affiliation: Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Müller
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D015723:Gene Library; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011486:Protein C / Q000378:metabolism; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D011486:Protein C
keywords: Activated protein C; Aptamer; CE-SELEX; Capillary electrophoresis; SELEX