title: The toxic effect of cypermethrin, amitraz and combinations of cypermethrin-amitraz in rats.
abstract: In this study, the effects of cypermethrin (CYP), amitraz (AMT) and combined cypermethrin-amitraz (CYP-AMT) on some serum biochemical, oxidative stress and drug-metabolising parameters were investigated in male Wistar albino rats. CYP, AMT and combined CYP-AMT were administered at doses of 80 mg kg(-1) bw(-1) of CYP and 170 mg kg(-1) bw(-1) of AMT for 1 day (single dose), and at doses of 12 mg kg(-1) bw(-1) of CYP and 25 mg kg(-1) bw(-1) of AMT for 40 days by oral gavage. Oxidative stress (malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G6PD)), serum biochemical (glucose, triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), creatinine, asparatate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein, albumin) in blood/tissues (liver, kidney, brain, spleen and testis) and hepatic drug-metabolising (cytochrome P450 2E1 (CYP2E1), NADH-cytochrome b5 reductase (CYPb5), NADPH-cytochrome c reductase/NADPH cytocrome P450 reductase (CYTC), glutathione S-transferase (GST), glutathione (GSH)) parameters were measured in liver samples taken on days 1 and 40. In result, it was determined that CYP, AMT and their combinations led to significant changes in the parameters investigated, and it was ascertained that long-term exposure to insecticides and the administration of insecticide combinations produced greater toxic effects in comparison with the administration of insecticides alone.
authors.affiliation: Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Erciyes University, Kayseri, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kanbur
authors.affiliation: Faculty of Medicine, Department of Biochemistry, Cumhuriyet University, Sivas, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Siliğ
authors.affiliation: Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Erciyes University, Kayseri, Turkey. geraslan38@hotmail.com.
authors.forename: Gökhan
authors.initials: G
authors.lastname: Eraslan
authors.affiliation: Safiye Çıkrıkcıoğlu Vacational Collage, Animal Health Department, Erciyes University, Kayseri, Turkey.
authors.forename: Mürsel
authors.initials: M
authors.lastname: Karabacak
authors.affiliation: Hakan Çetinsaya Experimantal Animal Center, Erciyes University, Kayseri, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Soyer Sarıca
authors.affiliation: Faculty of Pharmacy, Department of Biochemistry, Cumhuriyet University, Sivas, Turkey.
authors.forename: Serap
authors.initials: S
authors.lastname: Şahin
mesh_terms: D000818:Animals; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects; D014052:Toluidines / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D011722:Pyrethrins; D014052:Toluidines; C017160:cypermethrin; C014983:amitraz
keywords: Amitraz; Combination; Cypermethrin; Rat; Toxicity