title: Candiduria in Catheter Associated Urinary Tract Infection with Special Reference to Biofilm Production.
abstract: BACKGROUND
  Urinary tract infections as a result of Candida species are becoming increasingly common in hospital settings. The association is higher in patients with prolonged urinary catheterization and also various pre-disposing factors.
  
  
  OBJECTIVE
  This study was done to look into the significance of candiduria in the catheterized patients and to perform microbial catheterization of yeast and biofilm detection by tube method to guide treatment protocol.
  
  
  METHODS
  This is a prospective study. One hundred urine samples were collected over a period of 3 months. Specimens included were those of patients presenting with nosocomial Urinary tract infection (UTI) after 72 hours of hospitalization. The urine samples obtained were immediately processed in microbiology laboratory by semi-quantitative method as per standard protocol. All yeast isolates were stored for further microbial characterization. Biofilm production was detected by tube method.
  
  
  RESULTS
  In the present study we observed that out of 100 samples obtained from catheterized patients presenting with nosocomial UTI 26% were caused by Candida species. Among the 26 Candida isolates 16 (61.53%) were non albicans Candida and 10(38.47%) were Candida albicans. Out 26 Candida isolates, 14(53.84%) of the candida isolates were found to produce biofilm. Biofilm production was found to occur more frequently among non albicans Candida 10(62.5%) than Candida albicans 4(40.0%).
  
  
  CONCLUSIONS
  The present study reiterates the presence of candiduria in catheterized patients. Non-albicans candida speices are replacing candida albicans as the predominant pathogen for nosocomial UTI. It was also observed that Biofilm formation is seen more frequently with non albicans candida species than with Candida albicans.
authors.affiliation: Student, Bangalore Medical College and Research Institute , Bangalore, India .
authors.forename: Mythreyi Shekar
authors.initials: MS
authors.lastname: Rishpana
authors.affiliation: Professor, Department of Microbiology, Bangalore Medical College and Research Institute , Bangalore, India .
authors.forename: Jyoti S
authors.initials: JS
authors.lastname: Kabbin
keywords: Candida spp.; Catheterization; Nosocomial Urinary tract infection