title: Protein tetrazinylation via diazonium coupling for covalent and catalyst-free bioconjugation.
abstract: An efficient and bench-stable reagent was synthesized for direct and covalent introduction of tetrazines onto target protein or virus surfaces, which can be further modified based on tetrazine-ene ligation to achieve fluorescence labelling or PEGylation under mild conditions.
authors.affiliation: State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. lvshanshan@mail.buct.edu.cn yilong@mail.buct.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Men
authors.forename: Shanshan
authors.initials: S
authors.lastname: Lv
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Chen
mesh_terms: D000818:Animals; D002417:Cattle; D003979:Diazonium Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D003979:Diazonium Compounds; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D009113:Muramidase