title: Platelet - derived CD154 antigen in patients with chronic kidney disease.
abstract: BACKGROUND
  CD154 is a surface glycoprotein present on activated platelets, lymphocytes and mast cells. It mediates the transmission of information between cells and initiates an inflammatory response. The interaction of CD154 with its receptor CD40 leads to increase in concentrations of soluble forms of both molecules (sCD154, sCD40), which has an important prognostic value in cardiovascular complications. The metabolic disorders in chronic kidney disease (CKD), chronic inflammation, increased oxidative stress and type of renal replacement therapy may influence on the balance of sCD154/sCD40 in plasma and blood platelets. The purpose of the reasearch was to analyse the concentrations of sCD154 antigen and sCD40 receptor in platelet pure plasma (PPP) and platelet rich plasma (PRP) of patients with CKD treated conservatively, haemodialysed and on petitoneal dialysis.
  
  
  METHODS
  The group examined comprised 141 patients with chronic kidney disease: in pre-dialysis stage (n = 68), haemodialysed (n = 38) and on peritoneal dialysis (n = 35). The concentrations of sCD154 and sCD40 in PRP and PPP were determined with an ELISA method. The biochemical parameters were obtained using colorimetric method.
  
  
  RESULTS
  The concentrations of sCD154 and sCD40 in PPP and PRP in examined group were significantly different depending on the method of renal replacement therapy. The haemodialysis procedure caused a significant increase in sCD40 concentration in PRP. The concentrations of sCD40 and sCD154 were correlated with lipid parameters.
  
  
  CONCLUSIONS
  The type of renal replacement therapy influences on platelet activation which may be a factor contributing to increased cardiovascular complications in patients suffering from CKD.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland. Electronic address: asia_stepniewska@wp.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Stępniewska
authors.affiliation: Department of Medical Analysis, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołęgowska
authors.affiliation: Department of Medical Analysis, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Chruściana
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Gołembiewska
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Malinowska-Jędraszczyk
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Marchelek-Myśliwiec
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Ciechanowski
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000502:physiology; D001792:Blood Platelets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019013:CD40 Antigens / Q000097:blood / Q000276:immunology; D023201:CD40 Ligand / Q000097:blood*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000502:physiology; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000276:immunology
chemical_list: D019013:CD40 Antigens; D023201:CD40 Ligand
keywords: Cardio-vascular disease; Chronic kidney disease; Heamodialysis; Peritoneal dialysis; Platelets