title: The critical points in induction of experimental autoimmune uveitis.
abstract: BACKGROUND
  Autoimmune uveitis is a leading cause of visual impairment in developed countries in patients of working age. Animal models of experimental autoimmune uveitis (EAU) have been established to serve as a useful template for novel therapeutic approaches.
  
  
  METHODS
  Experimental autoimmune uveitis is induced in C57BL/6 mice by subcutaneous application of interphotoreceptor retinoid binding protein in complete Freund's adjuvant and pertussis toxin. Clinical and histological grading is used to assess the inflammation intensity of EAU.
  
  
  RESULTS
  The protocol of induction of EAU in mice hides several important aspects, which are crucial for developing the disease. These details have to be addressed to ensure reproducible disease induction. We describe our experience in establishing the model by pointing out the critical steps in EAU protocol which we found important.
  
  
  CONCLUSIONS
  The mouse model of EAU has practical value for preclinical studies, is robust and well established. However, the induction of inflammation of the eye can be quite challenging when important details of the protocol are not recognized and adhered to.
authors.affiliation: Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic.
authors.forename: Aneta
authors.initials: A
authors.lastname: Klimova
authors.affiliation: Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Seidler Stangova
authors.affiliation: Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Svozilkova
authors.affiliation: Section of Immunology and Infection, Institute of Medical Sciences, University of Aberdeen, United Kingdom.|Immunology and Virology Program, Centre for Ophthalmology and Visual Science, The University of Western Australia, Crawley, Western Australia, Australia and Centre for Experimental Immunology, Lions Eye Institute, Nedlands, Western Australia, Australia.
authors.forename: John V
authors.initials: JV
authors.lastname: Forrester
authors.affiliation: Section of Immunology and Infection, Institute of Medical Sciences, University of Aberdeen, United Kingdom.
authors.forename: Izabela
authors.initials: I
authors.lastname: Klaska
authors.affiliation: Department of Ophthalmology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Czech Republic.
authors.forename: Jarmila
authors.initials: J
authors.lastname: Heissigerova
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced*; D002908:Chronic Disease; D004195:Disease Models, Animal; D005136:Eye Proteins; D005260:Female; D005620:Freund's Adjuvant; D007509:Irritants; D008810:Mice, Inbred C57BL; D037342:Pertussis Toxin; D012177:Retinol-Binding Proteins; D014605:Uveitis / Q000139:chemically induced*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D000276:Adjuvants, Immunologic; D005136:Eye Proteins; D005620:Freund's Adjuvant; D007509:Irritants; D037342:Pertussis Toxin; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial
keywords: C57BL/6 mouse strain; chronic posterior autoimmune uveitis; experimental autoimmune uveitis; induction protocol; mouse model