title: Improvingz-tracking accuracy in the two-photon single-particle tracking microscope.
abstract: Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we have precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectoriesin silico. Our method can be generally applied to other 3D single-particle tracking techniques.
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Perillo
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: N
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Dunn
authors.affiliation: Department of Biomedical Engineering, University of Texas at Austin , Austin, Texas 78712, USA.
authors.forename: H-C
authors.initials: HC
authors.lastname: Yeh