title: Naphthalimide-based fluorescent probe for selectively and specifically detecting glutathione in the lysosomes of living cells.
abstract: A novel naphthalimide-based fluorescent probe employing a sulfonamide unit as a thiol-responsive group is reported. It is capable of efficiently distinguishing GSH from cysteine and homocysteine. Bioimaging shows that it has high selectivity in living cells and can visualize the level of GSH in lysosomes. It is worth mentioning that different groups on the imide unit can affect the selectivity and reaction dynamics of the probe towards thiols.
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China. yinj@mail.ccnu.edu.cn.
authors.forename: Meijiao
authors.initials: M
authors.lastname: Cao
authors.affiliation: Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing 210009, P. R. China. chenhaiyan@cpu.edu.cn.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing 210009, P. R. China. chenhaiyan@cpu.edu.cn.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China. yinj@mail.ccnu.edu.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China. yinj@mail.ccnu.edu.cn.
authors.forename: Sheng Hua
authors.initials: SH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, Nanjing 210009, P. R. China. chenxq@njtech.edu.cn.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China. yinj@mail.ccnu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry*; D053644:Naphthalimides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013449:Sulfonamides / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D013438:Sulfhydryl Compounds; D013449:Sulfonamides; D005978:Glutathione