title: Polymyxin B immobilized on cross-linked cellulose microspheres for endotoxin adsorption.
abstract: Cross-linked cellulose microspheres (CL-CMs) were successfully prepared by inverse crosslinking suspension method. NaOH/urea aqueous solution was used as solvent to dissolve cellulose at low temperature. The microspheres presented good spherical shape and monodispersity, which were applied to synthesize endotoxin adsorbent with polymyxin B (PMB) as ligand. The adsorbent showed good adsorption capability on endotoxin in physiologic saline solution and the maximum adsorption capacity was 3605 EU/g (1 EU=100 pg). It was worth noting that more than 70% of endotoxin could be effectively removed from the human plasma with the initial concentration of endotoxin ranged from 1 EU/mL to 5 EU/mL. The dynamic adsorption efficiency of endotoxin was 72.3% at the plasma perfusion rate of 300 mL/h with the endotoxin concentration of 4 EU/mL, while the variation of plasma protein before and after adsorption was only 8.9%. It suggests that the PMB immobilized CL-CMs have great potential application in clinical blood purification.
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cao
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Biyan
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, PR China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China. Electronic address: donghua@scut.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Dong
mesh_terms: D000327:Adsorption; D002072:Butylene Glycols / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry*; D004731:Endotoxins / Q000097:blood / Q000737:chemistry*; D006801:Humans; D008863:Microspheres; D011112:Polymyxin B / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002072:Butylene Glycols; D004731:Endotoxins; D014867:Water; D012972:Sodium Hydroxide; D014508:Urea; D002482:Cellulose; C014376:1,4-bis(2,3-epoxypropoxy)butane; D011112:Polymyxin B
keywords: Blood purification; Cellulose microspheres; Endotoxin; Inverse crosslinking suspension; PMB