title: Involvement of cyclic-nucleotide response element-binding family members in the radiation response of Ramos B lymphoma cells.
abstract: The aim of the present study was to investigate the role of Cyclic-nucleotide Response Element-Binding (CREB) family members and related nuclear transcription factors in the radiation response of human B lymphoma cell lines (Daudi and Ramos). Unlike the more radiosensitive Daudi cells, Ramos cells demonstrated only a moderate increase in early apoptosis after 3-5 Gy irradiation doses, which was detected with Annexin V/PI staining. Moreover, a significant and dose-dependent G2/M phase accumulation was observed in the same cell line at 24 h after both ionizing radiation (IR) doses. Western blot analysis showed an early increase in CREB protein expression that was still present at 3 h and more evident after 3 Gy IR in Ramos cells, along with the dose-dependent upregulation of p53 and NF-κB. These findings were consistent with real-time RT-PCR analysis that showed an early- and dose-dependent upregulation of NFKB1, IKBKB and XIAP gene expression. Unexpectedly, pre-treatment with SN50 did not increase cell death, but cell viability. Taken together, these findings let us hypothesise that the early induction and activation of NF-κB1 in Ramos cells could mediate necrotic cell death and be linked to other molecules belonging to CREB family and involved in the cell cycle regulation.
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Nisio
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Sancilio
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Di Giacomo
authors.affiliation: Molecular Genetics Institute CNR, I-27100 Pavia, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Rapino
authors.affiliation: Department of Medicine and Ageing Sciences, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Sancillo
authors.affiliation: Institute of Oncologic Radiotherapy, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Genovesi
authors.affiliation: Sanitary Physics Unit, SS. Annunziata Hospital, I-66100 Chieti, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Di Siena
authors.affiliation: Department of Medicine and Ageing Sciences, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Rosa Alba
authors.initials: RA
authors.lastname: Rana
authors.affiliation: Department of Pharmacy, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.affiliation: Department of Medicine and Ageing Sciences, G. d'Annunzio University, I-66100 Chieti, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Di Pietro
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D005720:Gamma Rays; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D016328:NF-kappa B