title: miR-490-5p suppresses tumour growth in renal cell carcinoma through targeting PIK3CA.
abstract: BACKGROUND
  Dysregulated micro-RNAs have been reported in many human cancers, including renal cell carcinoma. Recent studies indicated that miR-490 is involved in tumour development and progression. However, the expression profile and function in renal cell carcinoma remains unknown.
  
  
  RESULTS
  Herein, we showed that miR-490-5p was down-regulated in renal cell carcinoma tissues and cells compared with the adjacent normal tissues and normal cells. We also provided evidence that miR-490-5p acts as a tumour suppressor in renal carcinoma in a variety of in vitro and in vivo assays. Mechanistically, miR-490-5p was verified to directly bind to 3' UTR of the PIK3CA mRNA and reduce the expression of PIK3CA at both mRNA and protein levels, which further inhibits phosphatidylinositol 3-kinase/Akt signalling pathway. We further showed that knockdown of PIK3CA can block the growth inhibitory effect of miR-490-5p, and over-expression of PIK3CA can reverse the inhibitory effect of miR-490-5p on renal cancer cell tumourigenicity.
  
  
  CONCLUSIONS
  Taken together, our results indicated for the first time that miR-490-5p functions as a tumour suppressor in renal carcinoma by targeting PIK3CA.
  
  
  CONCLUSIONS
  Our findings suggest that miR-490-5p may be a potential gene therapy target for the treatment of renal cell carcinoma.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Tang
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Haibing
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Hu
authors.affiliation: College of Basic Medicine Science, Hubei University of Chinese Medicine, 430065, PR China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Yao
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Gan
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, PR China.|Hubei Institute of Urology, Wuhan, 430030, PR China.
authors.forename: Zhangqun
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D058534:Class I Phosphatidylinositol 3-Kinases; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*
chemical_list: C574862:MIRN490 microRNA, human; C000589011:MIRN490 microRNA, mouse; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human; C546639:Pik3ca protein, mouse
keywords: PIK3CA; Renal cell carcinoma; Tumour suppressor; miR-490-5p