title: Protective effects against hepatic ischemia-reperfusion injury after rat orthotopic liver transplantation because of BCL-2 overexpression.
abstract: This study aims to investigate the protective effects and mechanism of recombinant adenovirus Ad.VSG-hBCL-2 towards ischemia/reperfusion injury in rat liver graft. Recombinant adenovirus Ad.VSG-hBCL-2 was injected into the donor rat liver of the experiment group through the portal vein, the laparotomy was performed for liver 36 h later, and the liver was save in lactated Ringer's solution at 4°C for 4 h, "two-cuff method" was used to perform the orthotopic liver transplantation. The bile secretion situations of two groups were observed 6 h after the portal vein reflow; the recipient rats were killed to detect the plasma levels of AST, ALT and LDH. And the expressions of Bcl-2 and TNF-α in liver tissue, and TUNEL assay was used to detect the apoptosis of liver tissue cells, electron microscopy was used to observe the changes of subcellular structures of liver tissue. 6 h after the surgery, the immunohistochemistry and Western Blot test showed that the Bcl-2 expression in the liver of the experiment group significantly increased than the control group, the bile secretion increased, the levels of AST, ALT and LDH were significantly lower, and the TNF-α expression increased significantly. The changes of cellular morphology of the experiment group were milder, and the apoptotic index was significantly lower than the control group. The portal vein-transfected recombinant adenovirus Ad.VSG-hBCL-2 could be effectively expressed in rat liver, and the high expressed Bcl-2 could reduce the ischemia/reperfusion injury in the transplanted liver.
authors.affiliation: Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University Huai'an 223300, Jiangsu, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Intensive Care Uunit, First Hospital Affiliated to Xinjiang Medical University Urumqi, 830054, Xinjiang, China.
authors.forename: Long
authors.initials: L
authors.lastname: Ma
authors.affiliation: Central Laboratory of Huai'an First People's Hospital, Nanjing Medical University Huai'an 223300, Jiangsu, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University Huai'an 223300, Jiangsu, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University Huai'an 223300, Jiangsu, China.
authors.forename: Tianfang
authors.initials: T
authors.lastname: Xia
authors.affiliation: Department of Intensive Care Uunit, First Hospital Affiliated to Xinjiang Medical University Urumqi, 830054, Xinjiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Intensive Care Uunit, First Hospital Affiliated to Xinjiang Medical University Urumqi, 830054, Xinjiang, China.
authors.forename: Xiangyou
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University Huai'an 223300, Jiangsu, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Pang
keywords: Bcl-2; Liver transplantation; gene transfection; ischemia/reperfusion injury; rat