title: Antidiabetic Activity of Ruellia tuberosa L., Role of α-Amylase Inhibitor: In Silico, In Vitro, and In Vivo Approaches.
abstract: Ruellia tuberosa L. is a folk remedy in the treatment of diabetes mellitus. However, its hypoglycemic activity has not been investigated so far. In the present study, the antidiabetic mechanism of the n-hexane fraction of methanolic extract (HFME) of this plant was investigated in silico, in vitro, and in vivo. In silico study was performed using AutoDock4.2 software. In vitro  α-amylase inhibitory activity was investigated by starch-iodine method. A single dose of 450 mg/kg HFME for 14 days was subjected to an antidiabetic screening in vivo by a multiple low dose streptozotocin (MLD-STZ) induced rats. Molecular modeling results show that Betulin exhibited noncompetitive α-amylase inhibitory activities. The effect of HFME elicited significant reductions of diabetic rat blood glucose. A single dose administration of HFME inhibited α-amylase activity in vivo (P < 0.01) compared to a diabetic control group. Moreover, this extract strongly inhibited the α-amylase activity in vitro (IC50 0.14 ± 0.005 mg/mL). It is concluded that HFME exerted an antidiabetic effect via α-amylase inhibitor. Our findings provide a possible hypoglycemic action of R. tuberosa L. as an alternative therapy in the management of diabetes.
authors.affiliation: Academy of Food and Pharmacy Analyst, Putra Indonesia Malang, Street Barito 5, East Java, Malang 65123, Indonesia.
authors.forename: Dyah
authors.initials: D
authors.lastname: Ratna Wulan
authors.affiliation: Department of Chemistry, Faculty of Science, Brawijaya University, Street Veteran, East Java, Malang 65145, Indonesia.
authors.forename: Edi
authors.initials: E
authors.lastname: Priyo Utomo
authors.affiliation: Department of Chemistry, Faculty of Science, Brawijaya University, Street Veteran, East Java, Malang 65145, Indonesia.
authors.forename: Chanif
authors.initials: C
authors.lastname: Mahdi