title: A Comparative Study on Two Cationic Porphycenes: Photophysical and Antimicrobial Photoinactivation Evaluation.
abstract: Over the last decades, the number of pathogenic multi-resistant microorganisms has grown dramatically, which has stimulated the search for novel strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT) is one of the promising alternatives to conventional treatments based on antibiotics. Here, we present a comparative study of two aryl tricationic porphycenes where photoinactivation efficiency against model pathogenic microorganisms is correlated to the photophysical behavior of the porphycene derivatives. Moreover, the extent of photosensitizer cell binding to bacteria has been assessed by flow cytometry in experiments with, or without, removing the unbound porphycene from the incubation medium. Results show that the peripheral substituent change do not significantly affect the overall behavior for both tricationic compounds neither in terms of photokilling efficiency, nor in terms of binding.
authors.affiliation: Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, E-08017 Barcelona, Spain. ruben.ruiz@iqs.url.edu.
authors.forename: Rubén
authors.initials: R
authors.lastname: Ruiz-González
authors.affiliation: Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, E-08017 Barcelona, Spain. montserrat.agut@iqs.url.edu.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Agut
authors.affiliation: Department of Biology, University of Padova, via U. Bassi 58/B, E-35121 Padova, Italy. elena.reddi@unipd.it.
authors.forename: Elena
authors.initials: E
authors.lastname: Reddi
authors.affiliation: Institut Químic de Sarrià, Universitat Ramon Llull, via Augusta 390, E-08017 Barcelona, Spain. santi.nonell@iqs.url.edu.
authors.forename: Santi
authors.initials: S
authors.lastname: Nonell
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000528:radiation effects; D002412:Cations / Q000737:chemistry*; D005434:Flow Cytometry; D008027:Light; D050296:Microbial Viability / Q000187:drug effects / Q000528:radiation effects; D015394:Molecular Structure; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology*; D011166:Porphyrins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D002412:Cations; D017319:Photosensitizing Agents; D011166:Porphyrins; C051407:porphycene
keywords: MRSA; P. aeruginosa; antimicrobial photoinactivation; flow cytometry; photodynamic therapy; photosensitizer; porphycene; singlet oxygen