title: Polymorphism in the IL-8 Gene Promoter and the Risk of Acne Vulgaris in a Pakistani Population.
abstract: Interleukin-8 (IL-8) is a well-known inflammatory chemokine and suggested to be involved in the development of acne vulgaris. This study investigates IL-8 plasma levels in acne patients and healthy controls and the molecular basis for the regulation of the IL-8 gene in a Pakistani population. Patients with acne vulgaris (n = 264) and healthy individuals (n = 264) were enrolled in this investigation. Plasma IL-8 levels were determined by enzyme-linked immunosorbent assay (ELISA). The genotyping for IL-8 gene was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Our data showed a statistically significant increase in IL-8 levels from acne patients compared with healthy subjects (154.2 ± 52.1 pg/mL in patients vs. 101.6 ± 33.5 pg/mL in controls, p<0.0001). The IL-8-251T>A (rs4073) polymorphism was significantly higher in patients with acne compared with the control group (p=0.013). There was a significant difference between the T and A alleles from acne cases and controls (odds ratio OR=1.6,95 % CI= 1.16-2.19, p=0.003). Logistic-regression analysis showed that the increased IL-8 levels, and the IL-8-251T>A polymorphism were significantly associated with acne. Our data suggest that the elevated IL-8 levels and the IL-8-251T>A polymorphism may be associated with acne vulgaris in the study population.
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan.
authors.forename: Sabir
authors.initials: S
authors.lastname: Hussain
authors.affiliation: Department of Internal Medicine, Shifa College of Medicine, Shifa International Hospital, H-8/4, Islamabad, 44000 Pakistan.
authors.forename: Tahir
authors.initials: T
authors.lastname: Iqbal
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan.
authors.forename: Irfan
authors.initials: I
authors.lastname: Sadiq
authors.affiliation: Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Chak Shazad, Islamabad-44000, Pakistan.
authors.forename: Saima
authors.initials: S
authors.lastname: Feroz
authors.affiliation: Department of Hematology/Medicine, Armed Forces Bone Marrow Transplant Centre, Rawalpindi, 46000 Pakistan.
authors.forename: Humayoon
authors.initials: H
authors.lastname: Shafique Satti
mesh_terms: D000152:Acne Vulgaris / Q000209:etiology / Q000235:genetics*; D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012306:Risk
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
keywords: Acne vulgaris; Association; Interleukin-8; Pakistan; Polymorphism