title: Chromatographic immunoassays: strategies and recent developments in the analysis of drugs and biological agents.
abstract: A chromatographic immunoassay is a technique in which an antibody or antibody-related agent is used as part of a chromatographic system for the isolation or measurement of a specific target. Various binding agents, detection methods, supports and assay formats have been developed for this group of methods, and applications have been reported that range from drugs, hormones and herbicides to peptides, proteins and bacteria. This review discusses the general principles and applications of chromatographic immunoassays, with an emphasis being given to methods and formats that have been developed for the analysis of drugs and biological agents. The relative advantages or limitations of each format are discussed. Recent developments and research in this field, as well as possible future directions, are also considered.
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Matsuda
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: Elliott
authors.initials: E
authors.lastname: Rodriguez
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.|Home Department: Department of Chemistry, Tumkur University, Tumkur, Karnataka 572103, India.
authors.forename: Doddavenkatanna
authors.initials: D
authors.lastname: Suresh
authors.affiliation: Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Hage
mesh_terms: D001685:Biological Factors / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*
chemical_list: D001685:Biological Factors; D004364:Pharmaceutical Preparations