title: Stelleralides D-J and Anti-HIV Daphnane Diterpenes from Stellera chamaejasme.
abstract: Bioassay-guided fractionation of a petroleum ether extract of the roots of Stellera chamaejasme led to the isolation of seven new (stelleralides D-J, 1-7) and 12 known (8-19) daphnane diterpenoids. The structures and relative configurations of 1-7 were established on the basis of extensive spectroscopic analysis, including HRESIMS and comprehensive NMR techniques. All isolates were evaluated for anti-HIV activity in MT4 cells. All compounds tested, except 2, showed anti-HIV activity, and, especially, five 1α-alkyldaphnane diterpenoids (3, 4, 5, 10, and 11) exhibited extremely potent anti-HIV activity, with EC50 values of 0.06-1.1 nM and selectivity index values of more than 10,000.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University , Shanghai 201203, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University , Shanghai 201203, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Duke University Medical Center , Box 2926, SORF, Durham, North Carolina 27710, United States.
authors.forename: Chin-Ho
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina , Chapel Hill, North Carolina 27599-7568, United States.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Natural Products Research Laboratories, UNC Eshelman School of Pharmacy, University of North Carolina , Chapel Hill, North Carolina 27599-7568, United States.|Chinese Medicine Research and Development Center, China Medical University and Hospital , Taichung, Taiwan.
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Fudan University , Shanghai 201203, People's Republic of China.
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Chen
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D029645:Thymelaeaceae / Q000737:chemistry
chemical_list: D019380:Anti-HIV Agents; D004224:Diterpenes; D004365:Drugs, Chinese Herbal; C000605749:stelleralide G