title: Development of Fluorescent Protein Probes Specific for Parallel DNA and RNA G-Quadruplexes.
abstract: We have developed fluorescent protein probes specific for parallel G-quadruplexes by attaching cyan fluorescent protein to the G-quadruplex-binding motif of the RNA helicase RHAU. Fluorescent probes containing RHAU peptide fragments of different lengths were constructed, and their binding to G-quadruplexes was characterized. The selective recognition and discrimination of G-quadruplex topologies by the fluorescent protein probes was easily detected by the naked eye or by conventional gel imaging.
authors.affiliation: School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
authors.forename: Dung Thanh
authors.initials: DT
authors.lastname: Dang
authors.affiliation: School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore. phantuan@ntu.edu.sg.
authors.forename: Anh Tuân
authors.initials: AT
authors.lastname: Phan
mesh_terms: D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D054856:G-Quadruplexes; D008164:Luminescent Proteins / Q000032:analysis* / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D012313:RNA / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D010455:Peptides; D012313:RNA; D004247:DNA
keywords: DNA; G-quadruplexes; RHAU; RNA; fluorescent probes; proteins