title: Genome Analysis of Kingella kingae Strain KWG1 Reveals How a β-Lactamase Gene Inserted in the Chromosome of This Species.
abstract: We describe the genome of a penicillinase-producing Kingella kingae strain (KWG1), the first to be isolated in continental Europe, whose bla(TEM-1) gene was, for the first time in this species, found to be chromosomally inserted. The bla(TEM) gene is located in an integrative and conjugative element (ICE) inserted in Met-tRNA and comprising genes that encode resistance to sulfonamides, streptomycin, and tetracycline. This ICE is homologous to resistance-conferring plasmids of K. kingae and other Gram-negative bacteria.
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bidet
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Basmaci
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Guglielmini
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Doit
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Jost
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France.
authors.forename: André
authors.initials: A
authors.lastname: Birgy
authors.affiliation: INSERM, IAME, UMR 1137, Paris, France Université Paris Diderot, Sorbonne Paris Cité, IAME, UMR 1137, Paris, France AP-HP, Laboratoire de Microbiologie, Hôpital Robert-Debré, Paris, France stephane.bonacorsi@rdb.aphp.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bonacorsi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000737:chemistry*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D063990:Gene Ontology; D016680:Genome, Bacterial; D006801:Humans; D017882:Kingella kingae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D058977:Molecular Sequence Annotation; D016870:Neisseriaceae Infections / Q000382:microbiology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D013307:Streptomycin / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013449:Sulfonamides; D001618:beta-Lactamases; D013752:Tetracycline; D013307:Streptomycin