title: Bisdemethoxycurcumin (BDMC) Alters Gene Expression-associated Cell Cycle, Cell Migration and Invasion and Tumor Progression in Human Lung Cancer NCI-H460 Cells.
abstract: OBJECTIVE
  Lung cancer is one of the most common malignancies and a predominant cause of cancer-related death. It can metastasize in almost all organs, and currently, while new cases are increasing, treatment is still insufficient. Bisdemethoxycurcumin (BDMC), one of the components of turmeric, has been known to possess biological activities. However, the effects of BDMC on the genetic level remain unclear.
  
  
  METHODS
  Human lung cancer NCI-H460 cells were treated with 35 μM BDMC for 24 h and cells were harvested for total RNA extraction. The purified RNA was used for cDNA synthesis, labeling, microarray hybridization, and flour-labeled cDNA on-chip hybridization. The expression Console software (Affymetrix) with default RNA parameters was used to detect and quantitate concentrations of fluorescent molecules. The key genes involved and their possible interaction pathways were analyzed by the GeneGo software.
  
  
  RESULTS
  Seven genes, such as CCNE2 (cyclin E), associated with cell cycle, were over 4-fold overexpressed, 22 genes, such as ERCC6L (excision repair cross-complementing rodent repair deficiency, complementation group 6-like) associated with DNA damage and repair, were from 3- to 4-fold overexpressed and 266, such as cell division cycle, S-phase associated kinase and associated with cell death, genes were from 2- to 3-fold overexpressed.
  
  
  CONCLUSIONS
  BDMC induced changes in gene expression that may reveal cytotoxic information on the genetic level while presenting novel biomarkers or targets for treatment of human lung cancer in the future.
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Yu
authors.affiliation: Department of Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Mei-Due
authors.initials: MD
authors.lastname: Yang
authors.affiliation: School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Hui-Yi
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Department of Nursing, St. Mary's Medicine Nursing and Management College, Yilan, Taiwan, R.O.C.
authors.forename: An-Cheng
authors.initials: AC
authors.lastname: Huang
authors.affiliation: School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Jing-Pin
authors.initials: JP
authors.lastname: Lin
authors.affiliation: School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Chao-Lin
authors.initials: CL
authors.lastname: Kuo
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Liu
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
authors.forename: Hsin-Chung
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Department of Radiology, China Medical University Hospital, Taichung, Taiwan, R.O.C. School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw styang@mail.cmu.edu.tw.
authors.forename: Su-Tso
authors.initials: ST
authors.lastname: Yang
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. Department of Biotechnology, Asia University, Wu Feng, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw styang@mail.cmu.edu.tw.
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D030024:Curcuma / Q000737:chemistry; D003474:Curcumin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004249:DNA Damage / Q000187:drug effects; D036381:Diarylheptanoids; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D046228:Microarray Analysis; D009361:Neoplasm Invasiveness / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D036381:Diarylheptanoids; D009363:Neoplasm Proteins; C034786:bisdemethoxycurcumin; D003474:Curcumin
keywords: Bisdemethoxycurcumin; cDNA; human lung cancer NCI-H460 cells