title: Transient junction anisotropies orient annular cell polarization in the Drosophila airway tubes.
abstract: In contrast to planes, three-dimensional (3D) structures such as tubes are physically anisotropic. Tubular organs exhibit a striking orientation of landmarks according to the physical anisotropy of the 3D shape, in addition to planar cell polarization. However, the influence of 3D tissue topography on the constituting cells remains underexplored. Here, we identify a regulatory network polarizing cellular biochemistry according to the physical anisotropy of the 3D tube geometry (tube cell polarization) by a genome-wide, tissue-specific RNAi screen. During Drosophila airway remodelling, each apical cellular junction is equipotent to establish perpendicular actomyosin cables, irrespective of the longitudinal or transverse tube axis. A dynamic transverse enrichment of atypical protein kinase C (aPKC) shifts the balance and transiently targets activated small GTPase RhoA, myosin phosphorylation and Rab11 vesicle trafficking to longitudinal junctions. We propose that the PAR complex translates tube physical anisotropy into longitudinal junctional anisotropy, where cell-cell communication aligns the contractile cytoskeleton of neighbouring cells.
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, S-10691 Stockholm, Sweden.
authors.forename: Chie
authors.initials: C
authors.lastname: Hosono
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, S-10691 Stockholm, Sweden.
authors.forename: Ryo
authors.initials: R
authors.lastname: Matsuda
authors.affiliation: Cambridge Systems Biology Centre, University of Cambridge, Cambridge CB2 1QR, UK.
authors.forename: Boris
authors.initials: B
authors.lastname: Adryan
authors.affiliation: Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, S-10691 Stockholm, Sweden.|ECCPS, University of Giessen, Aulweg 130 35392 Giessen, Germany.
authors.forename: Christos
authors.initials: C
authors.lastname: Samakovlis
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D056151:Airway Remodeling; D000818:Animals; D030801:Animals, Genetically Modified; D016880:Anisotropy; D002450:Cell Communication; D016764:Cell Polarity; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000196:embryology / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005260:Female; D053263:Gene Regulatory Networks; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007365:Intercellular Junctions / Q000378:metabolism*; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D012137:Respiratory System / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism; D000099193:rab11 GTP-Binding Proteins
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins; D009218:Myosins; D011493:Protein Kinase C; D020691:rab GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein; C115015:PKC-3 protein; C559123:Rab11 protein, Drosophila; D000099193:rab11 GTP-Binding Proteins