title: Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.
abstract: The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally-derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.
authors.affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.
authors.forename: Nathan
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.
authors.forename: Jenna
authors.initials: J
authors.lastname: Richter
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, United States.
authors.forename: Josh
authors.initials: J
authors.lastname: Cutts
authors.affiliation: Department of Pediatrics, University of California, San Diego, San Diego, United States.
authors.forename: Kevin T
authors.identifier: http://orcid.org/0000-0001-5889-2847
authors.initials: KT
authors.lastname: Bush
authors.affiliation: Department of Pediatrics, University of California, San Diego, San Diego, United States.
authors.forename: Cleber
authors.initials: C
authors.lastname: Trujillo
authors.affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.|Department of Pediatrics, University of California, San Diego, San Diego, United States.
authors.forename: Sanjay K
authors.initials: SK
authors.lastname: Nigam
authors.affiliation: Scripps Institution of Oceanography, Scripps Genome Center, University of California, San Diego, San Diego, United States.
authors.forename: Terry
authors.initials: T
authors.lastname: Gaasterland
authors.affiliation: School of Biological and Health Systems Engineering, Arizona State University, Tempe, United States.
authors.forename: David
authors.initials: D
authors.lastname: Brafman
authors.affiliation: Department of Cellular and Molecular Medicine, University of California, San Diego, San Diego, United States.
authors.forename: Karl
authors.initials: K
authors.lastname: Willert
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D008648:Mesoderm; D039904:Pluripotent Stem Cells / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D023822:Tissue Engineering
keywords: arrayed cellular microenvironment; developmental biology; differentiation; human; intermediate mesoderm; pluripotent stem cell; stem cells