title: Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
abstract: Although an expansion of distinct γδ T-lymphocyte subpopulations has been shown after Salmonella infection of chicks, functions and activation states of these cells are still largely unknown. To characterize the activation status of avian γδ T lymphocytes after Salmonella exposure, an avian whole-blood Salmonella Enteritidis live-vaccine-strain (SE-LV) stimulation assay and flow cytometry were used, and the bacteria-provoked CD25-antigen expression of CD8-characterized γδ T-cell subsets determined in comparison to αβ T cells. In non-stimulated controls, low numbers of CD25-positive cells were observed in all T-cell populations. The SE-LV stimulation resulted in a significantly enhanced percentage of CD25-expressing CD8α(-), CD8α(lo+) and CD8αα(hi+) γδ T cells accompanied by an increased intensity of the CD25 expression of these cells. Highest values were always seen within the CD8αα(hi+) γδ T-lymphocyte subset. The αβ T cells showed no changed CD25 expression. In conclusion, the increased CD25 expression on discrete peripheral T-lymphocyte populations after ex vivo stimulation demonstrates a Salmonella-induced activation of γδ T-cell subsets and underlines the unique, probably activated character, especially of the avian CD8αα(hi+) γδ T cells.
authors.affiliation: Institute of Molecular Pathogenesis, 'Friedrich-Loeffler-Institut' (Federal Research Institute for Animal Health), Jena, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Braukmann
authors.affiliation: Institute of Bacterial Infections and Zoonoses, 'Friedrich-Loeffler-Institut' (Federal Research Institute for Animal Health), Jena, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Methner
authors.affiliation: Institute of Molecular Pathogenesis, 'Friedrich-Loeffler-Institut' (Federal Research Institute for Animal Health), Jena, Germany. Electronic address: angela.berndt@fli.bund.de.
authors.forename: Angela
authors.initials: A
authors.lastname: Berndt
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism; D002645:Chickens / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D066298:In Vitro Techniques; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008213:Lymphocyte Activation; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control; D022562:Salmonella Vaccines / Q000008:administration & dosage; D012477:Salmonella enteritidis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D016827:CD8 Antigens; C431698:CD8alpha antigen; D053645:Interleukin-2 Receptor alpha Subunit; D016692:Receptors, Antigen, T-Cell, gamma-delta; D022562:Salmonella Vaccines
keywords: CD25(+) γδ T cells; Chicken; Salmonella; Whole blood