title: A novel method to derive and expand mice neural stem cells efficiently without neuro-sphere formation.
abstract: Neural stem cells (NSCs) are multi-potent stem cells able to self-renew and generate immature and differentiated cell populations by asymmetric division. The NSCs are of considerable interest for cell replacement in neuro-degenerative diseases. NSCs are usually identified and expanded by their ability to generate free-floating aggregates termed neurospheres. However, neurospheres are not a pure population of NSCs with as little as 1% population in primary spheres. Neurospheres also contain neurons, astrocytes and oligodendrocytes. The heterogeneity of these cells may hinder their repopulation potential when used in cell transplantation. Furthermore, to obtain 1 million NSCs by the neurosphere protocol usually takes one month, which is inconvenient for future clinical trials. In this study, we tried to derive the NSCs from mice embryo neuroepithelium without neurosphere formation. Three different protocols were compared. We generated a direct and efficient NSCs generation, expanding and freezing protocol. This protocol can provide sufficient amount of the NSCs from first a few passages for cell transplantation.
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University Shijiazhuang, Hebei Province 050000, P.R. China.
authors.forename: Zhi-Zhao
authors.initials: ZZ
authors.lastname: Ma
authors.affiliation: Tongji University Medical School Siping Road 1239, Yangpu District 200092, Shanghai, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Thoracic Surgery, Lixian Hospital Ziwei East Road 666, Baoding City 071400, P.R. China.
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Huang
authors.affiliation: Tongji University Medical School Siping Road 1239, Yangpu District 200092, Shanghai, P.R. China ; Fountain Biotechnological (Yantai) Corporation Jinshajiang Road 163, Economic and Technology Development Zone, Yantai City, Shandong Province 264006, P.R. China.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Pan
keywords: Neural stem cell; differentiation; neuro-sphere; neuron