title: Protective effects of HGF gene-expressing human mesenchymal stem cells in acetaminophen-treated hepatocytes.
abstract: Mesenchymal stem cells (MSC) secrete a great variety of cytokines that have beneficial paracrine actions. Hepatocyte growth factor (HGF) promotes proliferation in several cell types. The aim of the present study was to investigate the protective effect of HGF gene-transfected MSC (HGF-MSC) in acetaminophen (AAP)-treated hepatocytes. We transfected the HGF gene into MSCs and confirmed HGF expression by RT-PCR and western blot. The concentration of HGF in HGF-MSC conditioned media (HGFCM) was upregulated compared with that in control MSCCM samples. Cell viability was increased in HGFCM-treated hepatocytes. Expression of Mcl-1, an anti-apoptosis protein, was increased and expression of pro-apoptosis proteins (Bad, Bik and Bid) was decreased in HGFCM-treated hepatocytes. HGF-MSC had protective effects on AAP-induced hepatocyte damage by enhancing proliferation. These results suggest that HGF-expressing MSCs may provide regenerative potential for liver cell damage.
authors.affiliation: a Department of Biological Science , Gachon University , Seongnam , South Korea and.
authors.forename: Yun Ho
authors.initials: YH
authors.lastname: Jang
authors.affiliation: a Department of Biological Science , Gachon University , Seongnam , South Korea and.
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: You
authors.affiliation: a Department of Biological Science , Gachon University , Seongnam , South Korea and.|b HanCell Inc. , Incheon , South Korea.
authors.forename: Myeong Jin
authors.initials: MJ
authors.lastname: Nam
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects* / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D051023:BH3 Interacting Domain Death Agonist Protein / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000293:injuries; D008565:Membrane Proteins / Q000096:biosynthesis; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D024101:Mitochondrial Proteins; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000096:biosynthesis; D014162:Transfection; D051025:bcl-Associated Death Protein / Q000096:biosynthesis
chemical_list: D018712:Analgesics, Non-Narcotic; D051017:Apoptosis Regulatory Proteins; C494819:BAD protein, human; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; C096820:BIK protein, human; D017077:Culture Media, Conditioned; C066855:HGF protein, human; C579095:MCL1 protein, human; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D051025:bcl-Associated Death Protein; D000082:Acetaminophen; D017228:Hepatocyte Growth Factor; D007770:L-Lactate Dehydrogenase
keywords: Acetaminophen; hepatocyte growth factor; mesenchymal stem cells; protection