title: Acetone-butanol-ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation.
abstract: Paper mill sludge (PS), a solid waste from pulp and paper industry, was investigated as a feedstock for acetone-butanol-ethanol (ABE) production by simultaneous saccharification and fermentation (SSF). ABE fermentation of paper sludge by Clostridium acetobutylicum required partial removal of ash in PS to enhance its enzymatic digestibility. Enzymatic hydrolysis was found to be a rate-limiting step in the SSF. A total of 16.4-18.0g/L of ABE solvents were produced in the SSF of de-ashed PS with solid loading of 6.3-7.4% and enzyme loading of 10-15FPU/g-glucan, and the final solvent yield reached 0.27g/g sugars. No pretreatment and pH control were needed in ABE fermentation of paper sludge, which makes it an attractive feedstock for butanol production. The results suggested utilization of paper sludge should not only consider the benefits of buffering effect of CaCO3 in fermentation, but also take into account its inhibitory effect on enzymatic hydrolysis.
authors.affiliation: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, United States.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, United States.
authors.forename: Suan
authors.initials: S
authors.lastname: Shi
authors.affiliation: Department of Biomedical, Chemical and Environmental Engineering, University of Cincinnati, 2901 Woodside Drive, Cincinnati, OH 45221, United States; Forest Products Laboratory and Center for Bioenergy and Bioproducts, Auburn University, 520 Devall Drive, Auburn, AL 36849, United States. Electronic address: tumg@uc.edu.
authors.forename: Maobing
authors.initials: M
authors.lastname: Tu
authors.affiliation: Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, United States.
authors.forename: Yoon Y
authors.initials: YY
authors.lastname: Lee
mesh_terms: D000096:Acetone / Q000378:metabolism*; D000468:Alkalies / Q000494:pharmacology; D000440:Butanols / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008897:Panicum / Q000187:drug effects; D010209:Paper; D012722:Sewage / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D000468:Alkalies; D000440:Butanols; D012722:Sewage; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; D014994:Xylose; D005947:Glucose
keywords: Acetone–butanol–ethanol (ABE); Clostridium acetobutylicum; Paper mill sludge; Simultaneous saccharification and fermentation (SSF)