title: Isolation, Identification and Partial Characterization of a Lactobacillus casei Strain with Bile Salt Hydrolase Activity from Pulque.
abstract: The aim of this study was to isolate, from pulque, Lactobacillus spp. capable of survival in simulated gastrointestinal stress conditions. Nine Gram-positive rods were isolated; however, only one strain (J57) shared identity with Lactobacillus and was registered as Lactobacillus casei J57 (GenBank accession: JN182264). The other strains were identified as Bacillus spp. The most significant observation during the test of tolerance to simulated gastrointestinal conditions (acidity, gastric juice and bile salts) was that L. casei J57 showed a rapid decrease (p ≤ 0.05) in the viable population at 0 h. Bile salts were the stress condition that most affected its survival, from which deoxycholic acid and the mix of bile salts (oxgall) were the most toxic. L. casei J57 showed bile salt hydrolase activity over primary and secondary bile salts as follows: 44.91, 671.72, 45.27 and 61.57 U/mg to glycocholate, taurocholate, glycodeoxycholate and taurodeoxycholate. In contrast, the control strain (L. casei Shirota) only showed activity over tauroconjugates. These results suggest that L. casei J57 shows potential for probiotic applications.
authors.affiliation: Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico. rakgova@yahoo.com.mx.|Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico. rakgova@yahoo.com.mx.
authors.forename: R
authors.initials: R
authors.lastname: González-Vázquez
authors.affiliation: Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Azaola-Espinosa
authors.affiliation: Departamento de Sistemas Biológicos, Maestría en Ciencias Farmacéuticas, Universidad Autónoma Metropolitana, Calzada del Hueso, 1100, Coyoacan, 04960, Mexico City, Mexico.
authors.forename: L
authors.initials: L
authors.lastname: Mayorga-Reyes
authors.affiliation: Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico.
authors.forename: L A
authors.initials: LA
authors.lastname: Reyes-Nava
authors.affiliation: Food and Nutritional Science Division, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Pok Fu Lam, Hong Kong.
authors.forename: N P
authors.initials: NP
authors.lastname: Shah
authors.affiliation: Departamento de Graduados e Investigación en Alimentos, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, 11340, Mexico City, Mexico.
authors.forename: Y
authors.initials: Y
authors.lastname: Rivera-Espinoza
mesh_terms: D027882:Agave / Q000382:microbiology*; D000581:Amidohydrolases / Q000378:metabolism*; D001628:Beverages / Q000382:microbiology*; D001647:Bile Acids and Salts / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D005285:Fermentation; D005750:Gastric Juice / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D019936:Probiotics
chemical_list: D001647:Bile Acids and Salts; D000581:Amidohydrolases; C023154:choloylglycine hydrolase
keywords: Bile salt hydrolase; Lactobacillus; Probiotics; Pulque