title: Identification of DLD, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three Vibrio species in Epinephelus coioides.
abstract: Vibrio spp. represent a serious threat to the culture of Epinephelus coioides (Orange-spotted Grouper) in Southeast Asia. In this study we used two-dimensional electrophoresis (2-DE) and Western blotting to identify common immunogenic proteins of Vibrio alginolyticus, Vibrio harveyi and Vibrio parahaemolyticus. Membranes were probed with orange-spotted grouper anti-V. alginolyticus sera and accordingly 60, 58 and 48 immunogenic protein spots were detected. By matching analysis for the three Western blotting membranes, 6 cross immunogenic spots for the three Vibrio species were identified. They were Outer membrane protein W (OmpW), dihydrolipoamide dehydrogenase (DLD), succinate dehydrogenase flavoprotein subunit(SDHA), elongation factor Ts(Ts), peptide ABC transporter periplasmic peptide-binding protein and phosphoenolpyruvate carboxykinase(PEPCK). One of the proteins, DLD, was used to evaluate the cross protective function for E. coioides with a bacterial immunization and challenge method. The relative percent survival rate of E. coioides against V. alginolyticus, V. harveyi and V. parahaemolyticus was 90%, 86% and 80%, respectively. This work may provide potential cross protective vaccine candidate antigens for three Vibrio species, and DLD may be considered as an effective cross-protective immunogen against three Vibrio species.
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China.
authors.forename: Huanying
authors.initials: H
authors.lastname: Pang
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Chen
authors.affiliation: Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK.
authors.forename: Rowena
authors.initials: R
authors.lastname: Hoare
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China; Zhongkai University of Agriculuture and Engineering, Guangzhou 510225, China.
authors.lastname: ZaoheWu
authors.affiliation: College of Fishery, Guangdong Ocean University, Zhanjiang 524025, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524025, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524025, China. Electronic address: jianjic@gmail.com.
authors.forename: Jichang
authors.initials: J
authors.lastname: Jian
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D001492:Bass; D056738:Cross Protection; D008058:Dihydrolipoamide Dehydrogenase / Q000276:immunology*; D005393:Fish Diseases / Q000517:prevention & control; D020543:Proteome; D011994:Recombinant Proteins / Q000276:immunology; D014733:Vibrio / Q000201:enzymology*; D014735:Vibrio Infections / Q000517:prevention & control / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D020543:Proteome; D011994:Recombinant Proteins; D008058:Dihydrolipoamide Dehydrogenase
keywords: Cross immunogenic proteins; DLD (dihydrolipoamide dehydrogenase); Immunoproteomic; Vibrio alginolyticus; Vibrio harveyi; Vibrio parahaemolyticus