title: [Antibacterial effect of the carboxymethyl chitosan zinc peptide on several periodontal pathogens in vitro].
abstract: OBJECTIVE
  To determine the antimicrobial effects of carboxymethyl chitosan zinc (CMC-Zn(+)) and CMC-Zn(+)-peptide (CMC-Zn(+)-P) on four kinds of periodontal pathogens.
  
  
  METHODS
  Dilution method was used to determine the minimum inhibitory concentration (MIC) of CMC-Zn(+) for Porphyromonas gingivalis (Pg), Actinobacillusactinomycetemcomitans (Aa), Prevotella intermedia (Pi) and Actinomyces viscosus (Av). The antimicrobial characters of CMC-Zn(+) and CMC-Zn(+)-P on these four kinds of pathogens were evaluated by disk diffusion method.
  
  
  RESULTS
  The MIC of CMC-Zn(+) for Pg, Aa, Pi and Av was 0.312 5%, 0.156 25%, 0.156 25% and 0.078 125% respectively. Significant antimicrobial effects were improved along with the increased concentration of CMC-Zn(+) and CMC-Zn(+)-P (P < 0.01), while the best antimicrobial concentrations of both CMC-Zn(+) and CMC-Zn(+)-P were 5%. The antimicrobial effect of CMC-Zn(+)-P was better than that of CMC-Zn(+) on the same kind of periodontal pathogen (P < 0.01). CMC-Zn(+)-P showed different antimicrobial effects on the four periodontal pathogens (Av > Aa > Pi or Av > Aa > Pg, P < 0.01).
  
  
  CONCLUSIONS
  CMC-Zn(+) and CMC-Zn(+)-P have inhibition effect on Pg, Aa, Pi and Av, and the best antibacterial concentration was 5%. The CMC-Zn(+)-P has better antibacterial effect than CMC-Zn(+) on Pg, Aa, Pi and Av.
authors.affiliation: Department of Oral Medcine, The Affiliated Hosipital of Qingdao University, Qingdao Shandong 266003, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.affiliation: Email: dengjing3333@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.forename: Wanshun
authors.initials: W
authors.lastname: Liu
authors.forename: Fulai
authors.initials: F
authors.lastname: Song
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents; D048271:Chitosan / Q000031:analogs & derivatives* / Q000494:pharmacology; D015169:Colony Count, Microbial; D008826:Microbial Sensitivity Tests; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D018713:Prevotella intermedia / Q000187:drug effects*; D015032:Zinc
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C514968:carboxymethyl-chitosan; D048271:Chitosan; D015032:Zinc