title: Selection, characterisation and evaluation of potential probiotic Lactobacillus spp. isolated from poultry droppings.
abstract: Aim of the present study was to characterise and evaluate probiotic potential of lactobacilli isolated from indigenous poultry. Lactobacilli were isolated from poultry droppings and identified by genus specific polymerase chain reaction and 16S rRNA gene sequencing. Isolates were characterised in vitro by their ability to tolerate low pH and bile salts, phytase activity, antimicrobial activity, antibiotic susceptibility profile, and autoaggregation and coaggregation with poultry gut pathogens. In vivo evaluation of selected isolates was done by their effect on the body weight gain and immune response of broiler chicks. Total of 90, one-day old chicks, were randomly divided in 9 groups and given selected lactobacilli alone and in combinations (108cfu/bird, daily) from day 7 to day 35. Body weight gain and humoral immune response to New Castle Disease Virus (NDV) vaccine were determined weekly. Three lactobacilli isolates (SMP52, SMP64 and SMP70) were selected as potentially probiotic bacteria on the basis of in vitro characterisation and identified as Lactobacillus crispatus, Lactobacillus casei and L. crispatus, respectively. Chicks supplemented with 'SMP52', 'SMP64', 'SMP70' and 'SMP64+SMP70' and a commercial probiotic product (Protexin) showed significantly higher mean weight gain per bird (1,584±35.2, 1,629±30.6, 1,668±34.7, 1,619±29.5 and 1,576±31.7 g/bird, respectively) as compared to negative control group (1,394±26.7 g/bird), on day 35. SMP 70 also showed significantly higher geometric mean titre against NDV vaccine at day 21 as compared to negative control. It is concluded that L. crispatus SMP52, L. casei SMP64 and L. crispatus SMP70 are potential probiotic candidates which alone or in different combinations may increase body weight of broilers.
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Asghar
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Arif
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Nawaz
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: K
authors.initials: K
authors.lastname: Muhammad
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: M A
authors.initials: MA
authors.lastname: Ali
authors.affiliation: 2 Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: M D
authors.initials: MD
authors.lastname: Ahmad
authors.affiliation: 3 Department of Food Science and Human Nutrition, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: S
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: A A
authors.initials: AA
authors.lastname: Anjum
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Khan
authors.affiliation: 1 Department of Microbiology, University of Veterinary and Animal Sciences, Out-Fall Road Lahore, 54000 Lahore, Pakistan.
authors.forename: J
authors.initials: J
authors.lastname: Nazir
mesh_terms: D010832:6-Phytase / Q000378:metabolism; D000222:Adaptation, Physiological; D000383:Agriculture; D000818:Animals; D001647:Bile Acids and Salts; D001835:Body Weight; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000302:isolation & purification*; D000070003:Lactobacillus crispatus / Q000235:genetics / Q000302:isolation & purification*; D009522:Newcastle disease virus / Q000276:immunology; D010154:Pakistan; D019936:Probiotics / Q000302:isolation & purification* / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D001647:Bile Acids and Salts; D012336:RNA, Ribosomal, 16S; D014765:Viral Vaccines; D010832:6-Phytase
keywords: Lactobacillus casei; Lactobacillus crispatus; antibacterial activity; bile tolerance