title: Multiple virus resistance using artificial trans-acting siRNAs.
abstract: Plant TAS gene encoded trans-acting siRNAs (ta-siRNAs) regulate the expression of target mRNAs by guiding their cleavage at the sequence complementary region as microRNAs. Since one TAS transcript is cleaved into multiple ta-siRNAs in a phased manner, TAS genes may be engineered to express multiple artificial ta-siRNAs (ata-siRNAs) that target multiple viruses at several distinct genomic positions. To test this hypothesis, the Arabidopsis TAS3a gene was engineered to express ata-siRNAs targeting the genome of Turnip mosaic virus (TuMV) and Cucumber mosaic virus (CMV). Transgenic Arabidopsis thaliana plants expressing these ata-siRNAs showed high level of resistance to both viruses. These results suggest that plant TAS genes can be modified to express artificial ta-siRNAs to confer multiple virus resistance and could have broad applications for future development in virus resistance strategies.
authors.affiliation: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, PR China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Life and Environmental Science, Hangzhou Normal University, 310036 Hangzhou, Zhejiang, PR China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, PR China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, PR China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhan
authors.affiliation: Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wu
authors.affiliation: Institute of Horticultural Sciences, Heilongjiang Academy of Agricultural Science, 150069 Harbin, Heilongjiang, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Agricultural and Food Science, Zhejiang Agricultural and Forestry University, Lin'an 311300, Zhejiang, PR China. Electronic address: wxy5551@126.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Wu
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000821:virology; D017799:Cucumovirus / Q000235:genetics / Q000502:physiology; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000138:chemical synthesis*; D017806:Tymovirus / Q000235:genetics / Q000502:physiology
chemical_list: D018749:RNA, Plant; D034741:RNA, Small Interfering; D015534:Trans-Activators
keywords: Artificial trans-acting siRNA; Cucumber mosaic virus; Multiple virus resistance; TAS; Turnip mosaic virus