title: [Role of SREBP-1c in risk of liver disease associated with the triacylglycerol lipase PNPLA3 I148M variant].
abstract: OBJECTIVE
  To investigate the relationship between SREBP-1c and the risk of liver disease associated with the triacylglyceride lipase PNPLA3 I148M variant using a human hepatoma cell line model transfected with recombinant lentiviruses.
  
  
  METHODS
  Huh7 cells were transfected with control lentivirus or lentivirus containing the PNPLA3 I148M variant (variant). The two cell groups were compared to assess differences in triglyceride content (using oil red O staining), levels of triglyceride and cholesterol (using automated biochemical analyzer), expression of SREBP-lc mRNA (using fluorescence quantitative PCR), and expression of SREBP-1c protein (using western blot.
  
  
  RESULTS
  Cells expressing the PNPLA3 I148M variant showed higher triglyceride content (0.54+/-0.03 mmol/L vs. control cells: 0.23+/-0.02 mmol/L; t=22.58, P<0.001), cholesterol level (0.28+/-0.03 mmol/L vs. control cells: 0.13+/-0.02 mmol/L; t =11.83, P<0.001), SREBP-1cmRNA expression (13.59+/-0.60 vs. 11.81+/-0.82; [The abstract and text in the paper say variant increases, but the data shown says the higher value is in the control cells. Please correct to properly express the data.] P=0.001), and SREBP-1c protein expression. The level of SREBP-1c was positively correlated with serum triglyceride in the cells expressing the PNPLA3 I148M variant (r=0.912, P<0.01).
  
  
  CONCLUSIONS
  The risk of liver disease associated with the PNPLA3 I148M variant, which increases lipogenesis, may involve SREBP-1c and a pathway that increases triglycerides.
authors.affiliation: Second Department of Gasteroenterology, Qingdao Municipal Hospital, Qingdao 260021, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.forename: Baiquan
authors.initials: B
authors.lastname: An
authors.forename: Yongning
authors.initials: Y
authors.lastname: Xin
authors.forename: Man
authors.initials: M
authors.lastname: Jiang
authors.forename: Wenwen
authors.initials: W
authors.lastname: Jin
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Lin
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Jiang
authors.forename: Shiying
authors.initials: S
authors.lastname: Xuan
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008049:Lipase; D008107:Liver Diseases; D008565:Membrane Proteins; D012307:Risk Factors; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D008049:Lipase; D008565:Membrane Proteins; D051780:Sterol Regulatory Element Binding Protein 1; D014280:Triglycerides; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent