title: [Diversity of faecal bacteria in several mammals by 454 high-throughput sequencing].
abstract: OBJECTIVE
  The aim of this study was to analyze the diversities and differences of several mammalian' s faecal bacteria, to understand the relationships between bacterium diversities and animals' evolutionary and animals' feeds.
  
  
  METHODS
  Genomic DNA of feces was extracted and amplified for the 16S rDNA V3 tags, and then the tags were sequenced by 454 sequencing. QIIME were used to analyze faecal bacterial diversities.
  
  
  RESULTS
  Faecal bacteria of all animals were dominated by Firmicutes, Bacteroidetes and Proteobacteria. Bacterial diversities of Hylobates hoolock, Pan troglodytes and Rhinopithecus roxellanae were the highest, followed by Panthera tigris altaica, Ailuropoda melanoleuca and Ursus thibetanus were the lowest through α diversity analysis. The constituents of faecal bacteria among Hylobates hoolock, Pan troglodytes and Rhinopithecus roxellanae were similar. The constituents of faecal bacteria among Ailuropoda melanoleuca, Ursus thibetanu, and Panthera tigris altaica were similar. Mainly for containning Fusobacteria, the faecal bacterial of Panthera tigris altaica differed from the other two carnivore animals through β diversity analysis.
  
  
  CONCLUSIONS
  The dominating faecal bacteria were obvious, the bacteria similarities of the two repetitions were the highest. The diversities of each animal were different and higher in the primates. Both evolution and food were related to faecal bacteria. This study provided some references for exploring the new microorganism and further research of faecal bacteria.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.forename: Qinyuan
authors.initials: Q
authors.lastname: Li
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Xue
authors.forename: Guiding
authors.initials: G
authors.lastname: Li
authors.forename: Man
authors.initials: M
authors.lastname: Jia
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiangl
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D000882:Haplorhini; D059014:High-Throughput Nucleotide Sequencing; D006922:Hylobates; D008322:Mammals / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D046010:Tigers; D001503:Ursidae
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S