title: Immunohistochemical expression of WNT5A and MMPs in odontogenic epithelial tumors and cysts.
abstract: OBJECTIVE
  The aim of this study was compare the expression of WNT5A and MMP2, 7 and 20, in frequent benign odontogenic tumors and odontogenic cysts, since these lesions have a different biological behavior.
  
  
  METHODS
  Eighty-one paraffin-embedded specimens of odontogenic tumors, including ameloblastoma and keratocystic odontogenic tumor, and thirty-two odontogenic cysts were used for immunohistochemical analysis.
  
  
  RESULTS
  The expression of WNT5A in odontogenic tumors and inflammatory cyst was higher than in developmental odontogenic cyst. There was no statistical difference (p<0.05) in the expression of WNT5A when comparing the analyzed tumors. The expression of MMP7 was lower in RC with a statistical difference when compared with all tumors and cysts. Statistical differences also occurred when comparing glandular odontogenic cyst (GOC) to keratocyst odontogenic tumor (KOT) and calcifying cystic odontogenic tumor (CCOT). MMP20 expression was higher in ameloblastoma when compared to adenomatoid odontogenic tumor (AOT), DC and GOC. The expression of MMP20 was lower in CCOT when compared to all tumors and cysts.
  
  
  CONCLUSIONS
  The expression of WNT5A in a group of odontogenic lesions suggests the participation of a non-canonical WNT signaling pathway in the progression and maintenance of these lesions. These molecules are possibly involved in the biological differences between odontogenic tumors and cysts. Considering previous studies, WNT5A may help promote the calcification seen in AOT, CCOT and CEOT by activating MMP7.
authors.affiliation: University of São Paulo, School of Dentistry, Department of Oral Pathology, Av Professor Lineu Prestes, 2227 São Paulo, Brazil.
authors.forename: Douglas Magno
authors.initials: DM
authors.lastname: Guimarães
authors.affiliation: University of São Paulo, School of Dentistry, Department of Oral Pathology, Av Professor Lineu Prestes, 2227 São Paulo, Brazil.
authors.forename: Daniella Moraes
authors.initials: DM
authors.lastname: Antunes
authors.affiliation: University of São Paulo, School of Dentistry, Department of Oral Pathology, Av Professor Lineu Prestes, 2227 São Paulo, Brazil.
authors.forename: Juvani Lago
authors.initials: JL
authors.lastname: Saturno
authors.affiliation: University of São Paulo, School of Dentistry, Department of Oral Pathology, Av Professor Lineu Prestes, 2227 São Paulo, Brazil.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Massuda
authors.affiliation: University of São Paulo, Biomedical Sciences Institute, Department of Anatomy, Av Professor Lineu Prestes, 2415 São Paulo, Brazil.
authors.forename: Katiúcia Batista da Silva
authors.initials: KB
authors.lastname: Paiva
authors.affiliation: University of São Paulo, School of Dentistry, Department of Oral Pathology, Av Professor Lineu Prestes, 2227 São Paulo, Brazil. Electronic address: fadnunes@usp.br.
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000564:Ameloblastoma / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000378:metabolism*; D008297:Male; D053519:Matrix Metalloproteinase 20 / Q000378:metabolism*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; C507111:MMP20 protein, human; D053519:Matrix Metalloproteinase 20
keywords: Adenomatoid odontogenic tumor; Ameloblastoma; Calcifying epithelial odontogenic tumor and calcifying cystic odontogenic tumor; Odontogenic tumors; WNT5A