title: Assay for CDP-Diacylglycerol Generation by CDS in Membrane Fractions.
abstract: CDP-DAG is a liponucleotide formed by the condensation of CTP with the phospholipid phosphatidic acid in a reaction catalyzed by CDP-DAG synthase (CDS). CDP-DAG is required for the synthesis of phosphatidylinositol; the parent molecule whence all seven phosphoinositides including the signaling molecules PI4P, PI(4,5)P2, and PI(3,4,5)P3 are derived. This protocol describes a highly sensitive radiometric assay to detect the generation of CDP-DAG on isolated biological membrane fractions.
authors.affiliation: School of Life and Medical Sciences, University College London, Royal Free Campus, Rowland Hill Street, London, NW3 2PF, UK. m.waugh@ucl.ac.uk.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Waugh
mesh_terms: D000818:Animals; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D003567:Cytidine Diphosphate Diglycerides / Q000096:biosynthesis*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D013347:Subcellular Fractions
chemical_list: D003567:Cytidine Diphosphate Diglycerides; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
keywords: Assay; CDP-DAG; CDS; Endoplasmic reticulum; Liponucleotide; Membrane