title: Proteomic Study to Survey the CIGB-552 Antitumor Effect.
abstract: CIGB-552 is a cell-penetrating peptide that exerts in vitro and in vivo antitumor effect on cancer cells. In the present work, the mechanism involved in such anticancer activity was studied using chemical proteomics and expression-based proteomics in culture cancer cell lines. CIGB-552 interacts with at least 55 proteins, as determined by chemical proteomics. A temporal differential proteomics based on iTRAQ quantification method was performed to identify CIGB-552 modulated proteins. The proteomic profile includes 72 differentially expressed proteins in response to CIGB-552 treatment. Proteins related to cell proliferation and apoptosis were identified by both approaches. In line with previous findings, proteomic data revealed that CIGB-552 triggers the inhibition of NF-κB signaling pathway. Furthermore, proteins related to cell invasion were differentially modulated by CIGB-552 treatment suggesting new potentialities of CIGB-552 as anticancer agent. Overall, the current study contributes to a better understanding of the antitumor action mechanism of CIGB-552.
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Arielis
authors.initials: A
authors.lastname: Rodríguez-Ulloa
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Jeovanis
authors.initials: J
authors.lastname: Gil
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Yassel
authors.initials: Y
authors.lastname: Ramos
authors.affiliation: Department of Bioinformatics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Lilian
authors.initials: L
authors.lastname: Hernández-Álvarez
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Lisandra
authors.initials: L
authors.lastname: Flores
authors.affiliation: Pharmaceutical Department, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Brizaida
authors.initials: B
authors.lastname: Oliva
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Dayana
authors.initials: D
authors.lastname: García
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Aniel
authors.initials: A
authors.lastname: Sánchez-Puente
authors.affiliation: Department of Bioinformatics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Alexis
authors.initials: A
authors.lastname: Musacchio-Lasa
authors.affiliation: Department of Bioinformatics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Jorge
authors.initials: J
authors.lastname: Fernández-de-Cossio
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Padrón
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Luis J
authors.initials: LJ
authors.lastname: González López
authors.affiliation: Department of Proteomics, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Besada
authors.affiliation: Pharmaceutical Department, Center for Genetic Engineering and Biotechnology, 10600 Havana, Cuba.
authors.forename: Maribel
authors.initials: M
authors.lastname: Guerra-Vallespí
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D057846:Cell-Penetrating Peptides / Q000008:administration & dosage* / Q000737:chemistry*; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods; D016896:Treatment Outcome
chemical_list: C000598873:CIGB-552 peptide; D057846:Cell-Penetrating Peptides; D009363:Neoplasm Proteins; D020543:Proteome