title: Hypolipidemic and Hepatic Steatosis Preventing Activities of the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes) Ethanol Extract In Vivo and In Vitro.
abstract: Obesity, a rapidly growing threat to human health worldwide, is responsible for a large proportion of the total burden of disease. Therefore, obesity control could be a vital scheme to prevent many diseases. The aim of this study was to examine the activities and mechanism of Auricularia auricula-judae 70% ethanol extract (AAE) in preventing hypolipidemic and hepatic steatosis. A normal diet (ND) and a high-fat diet (HFD) with or without 0.1% (w/w), 0.3% (w/w), and 1% (w/w) AAE were given to male C57BL/6 mice. Plasma lipids and liver enzymes were measured and tissue sections of liver were examined. Further mechanistic studies of mouse 3T3-L1 adipocytes were performed in vitro by verifying triglyceride, glycerol, and glycerol-3-phosphate dehydrogenase activity and messenger RNA expression of adipogenic and lipogenic genes using reverse transcriptase polymerase chain reaction amplification. Body weight and adipose tissue mass were significantly reduced in mice fed an ND and a HFD plus AAE compared with mice fed an HFD. In AAE-supplemented groups, plasma lipids and liver enzymes decreased dose-dependently. AAE suppressed the expression of adipogenic/lipogenic genes (PPARγ, C/EBPα, FAS) in 3T3-L1 cells without cytotoxicity. These findings suggest that AAE may reduce the risk of hepatic steatosis by modulating plasma lipids via the regulation of adipogenic/lipogenic transcriptional factors. AAE may have interesting applications to improve plasma lipids and liver enzymes.
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University (Barisal Campus), Babugonj, Barisal, Bangladesh.
authors.forename: Md Ahsanur
authors.initials: MA
authors.lastname: Reza
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Md Akil
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Dereje
authors.initials: D
authors.lastname: Damte
authors.affiliation: Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-701, Republic of Korea.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jo
authors.affiliation: Department of Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
authors.forename: Walter H
authors.initials: WH
authors.lastname: Hsu
authors.affiliation: Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Kyungpook National University, Daegu 702701, Republic of Korea.
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D001786:Blood Glucose / Q000187:drug effects; D001798:Blood Proteins / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology; D059305:Diet, High-Fat; D005234:Fatty Liver / Q000097:blood / Q000517:prevention & control*; D015870:Gene Expression / Q000187:drug effects; D000960:Hypolipidemic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D007009:Hypolipoproteinemias / Q000517:prevention & control*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D003603:Cytotoxins; D000960:Hypolipidemic Agents; D008055:Lipids