title: Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
abstract: This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor. In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners. For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures. Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Academy of Agricultural Sciences, Shandong 271018, China.
authors.forename: Yongheng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Xu
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Life Science, Shandong Agricultural University, Shandong 271018, China. Electronic address: jia__le@126.com.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D025282:Mycelium / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry*; D012210:Rhamnose / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014994:Xylose / Q000737:chemistry; D015032:Zinc / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D014994:Xylose; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D015032:Zinc; D012210:Rhamnose
keywords: Antioxidant and hepatoprotective effects; Characterization; Mycelia zinc polysaccharides; Pleurotus djamor; Purification