title: Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study.
abstract: Metabolites are the end products of cellular regulatory processes. Squamous cervical cancer (SCC) can alter the level of certain small molecular metabolite in plasma through modulating gene expression. In this study, we identified two metabolites, phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), which are significantly down- and upregulated in plasma of SCC as compared to uterine fibroid (UF) patients via ultra-performance liquid chromatographic-mass spectrometry (UPLC-MS). In external prospective cohort, our assay has a sensitivity of 93.2 %, a specificity of 91.3 %, and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.972. The level of LPC is significantly higher in SCC than in UF patients. An opposite result was observed in PC level. Our findings suggest that the PC and lysoPC could be used as novel biomarkers to facilitate SCC diagnosis.
authors.affiliation: State Key Laboratory of Natural Products, Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198, People's Republic of China.|Department of Pathology, Yale School of Medicine, New Haven, USA.
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Yin
authors.affiliation: Department of Gynecology Oncology, The Tumor Affiliated Hospital of Harbin Medical University, Harbin, 150086, People's Republic of China.|Department of Pathology, Yale School of Medicine, New Haven, USA.
authors.forename: Shu
authors.initials: S
authors.lastname: Tan
authors.affiliation: Department of Pathology, Yale School of Medicine, New Haven, USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Epidemiology and Biostatistics, Harbin Medical University, Harbin, 150081, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Natural Products, Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198, People's Republic of China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Epidemiology and Biostatistics, Harbin Medical University, Harbin, 150081, People's Republic of China. likang@ems.hrbmu.edu.cn.
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.affiliation: State Key Laboratory of Natural Products, Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing, 211198, People's Republic of China. junpingkou@cpu.edu.cn.
authors.forename: Junping
authors.initials: J
authors.lastname: Kou
authors.affiliation: Department of Gynecology Oncology, The Tumor Affiliated Hospital of Harbin Medical University, Harbin, 150086, People's Republic of China. yinlouge@126.com.
authors.forename: Ge
authors.initials: G
authors.lastname: Lou
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000097:blood / Q000473:pathology; D008244:Lysophosphatidylcholines / Q000097:blood*; D013058:Mass Spectrometry; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood*; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines
keywords: Biomarker; Cervical cancer; Lysophosphatidylcholine; Metabonomics; Phosphatidylcholine