title: Biowire platform for maturation of human pluripotent stem cell-derived cardiomyocytes.
abstract: Human pluripotent stem cells (hPSCs)-derived cardiomyocytes (hPSC-CMs) represent a potential indefinite cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, these cells are immature compared with adult ventricular cardiomyocytes. In order to overcome this limitation, an engineered platform, called biowire, was devised to provide cultured cardiomyocytes important biomimetic cues present during embryo development, such as three-dimensional cell culture, extracellular matrix composition, soluble factors and pacing through electrical stimulation, to induce the maturation of hPSC-CMs in vitro.
authors.affiliation: University Health Network, Toronto General Research Institute, 101 College St., Canada.
authors.forename: Xuetao
authors.initials: X
authors.lastname: Sun
authors.affiliation: University Health Network, Toronto General Research Institute, 101 College St., Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Canada; Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, Canada. Electronic address: sara.vasconcelos@utoronto.ca.
authors.forename: Sara S
authors.initials: SS
authors.lastname: Nunes
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D004558:Electric Stimulation; D006801:Humans; D032383:Myocytes, Cardiac / Q000502:physiology*; D039904:Pluripotent Stem Cells / Q000502:physiology*
keywords: Biomimetic cues; Cardiomyocyte; Maturation; Stem cells; Tissue engineering