title: Neutral monosaccharide composition analysis of plant-derived oligo- and polysaccharides by high performance liquid chromatography.
abstract: A novel analytical method for neutral monosaccharide composition analysis of plant-derived oligo- and polysaccharides was developed using hydrophilic interaction liquid chromatography coupled to a charged aerosol detector. The effects of column type, additives, pH and column temperature on retention and separation were evaluated. Additionally, the method could distinguish potential impurities in samples, including chloride, sulfate and sodium, from sugars. The results of validation demonstrated that this method had good linearity (R(2) ≥ 0.9981), high precision (relative standard deviation ≤ 4.43%), and adequate accuracy (94.02-103.37% recovery) and sensitivity (detection limit: 15-40 ng). Finally, the monosaccharide compositions of the polysaccharide from Eclipta prostrasta L. and stachyose were successfully profiled through this method. This report represents the first time that all of these common monosaccharides could be well-separated and determined simultaneously by high performance liquid chromatography without additional derivatization. This newly developed method is convenient, efficient and reliable for monosaccharide analysis.
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Songshan
authors.initials: S
authors.lastname: Shi
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Liu
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China. Electronic address: shunchunwang@126.com.
authors.forename: Shunchun
authors.initials: S
authors.lastname: Wang
mesh_terms: D002712:Chlorides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036601:Eclipta / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D002712:Chlorides; D009005:Monosaccharides; D009844:Oligosaccharides; D011134:Polysaccharides; D013431:Sulfates; C005695:stachyose; D012964:Sodium
keywords: Carbohydrates; Charged aerosol detector; Eclipta prostrasta L.; Hydrophilic interaction liquid chromatography; Monosaccharide composition