title: Galactosylation of steroidal saponins by β-galactosidase from Lactobacillus bulgaricus L3.
abstract: The galactosylation of furostanosides and spirostanosides were investigated by using β-galactosidase from Lactobacillus bulgaricus L3 as a catalyst and lactose as a sugar donor. Four novel galactosylated furostanoside products (compounds 1-4) from compound F, compound G, compound I, and compound H were obtained and their structures were identified by HR-ESI-MS, 1D and 2D NMR spectra. The result showed that galactosyl moiety was found to be added to the 6-OH of the 26-O-glucosyl in these four furostanoside substrates.
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.|Ovation Health Science and Technology Co. Ltd., ENN Group, Langfang, 065001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda South Road, Jinan, 250100, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Lu
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Dao-di Herbs, National Resource Center of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 16# Nanxiaojie, Dongcheng District, Beijing, 100700, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Kang
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Pang
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China.
authors.forename: Tengchuan
authors.initials: T
authors.lastname: Jiang
authors.affiliation: State Key Lab of Microbial Technology and National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda South Road, Jinan, 250100, China. minxiao@sdu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Xiao
authors.affiliation: Beijing Institute of Radiation Medicine, 27# Tai-ping Road, Haidian District, Beijing, 100850, China. mabaiping@sina.com.
authors.forename: Baiping
authors.initials: B
authors.lastname: Ma
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D005690:Galactose / Q000031:analogs & derivatives*; D007778:Lactobacillus / Q000201:enzymology*; D012503:Saponins / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012503:Saponins; D001616:beta-Galactosidase; D005690:Galactose
keywords: Galactosylation; Lactobacillus bulgaricus L3; Steroidal saponins; β-galactosidase