title: The role for human nasal epithelial nuclear factor kappa B activation in histamine-induced mucin 5 subtype B overproduction.
abstract: BACKGROUND
  Mucin 5 subtype B (MUC5B) is 1 of the major mucins secreted by airway epithelial cells. We sought to determine the effect of histamine on MUC5B expression in human nasal epithelial cells.
  
  
  METHODS
  Human nasal epithelial cells from allergic rhinitis patients were cultured, and stimulated with 4 concentrations of histamine, or pretreated with a specific nuclear factor-kappa B (NF-κB) inhibitor (Bay11-7082) before histamine stimulation. Immunocytochemistry and Western blotting were used to detect phosphorylated inhibitor of kappa B alpha (p-IκBα), NF-κBp65 and MUC5B protein. MUC5B content in supernatants was assayed by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Histamine promoted IκBα phosphorylation and NF-κBp65 nuclear translocation. A concentration-dependent histamine-induced increase of MUC5B protein was observed, and its content in supernatants was upregulated in a concentration-dependent fashion, but these effects were attenuated by Bay11-7082.
  
  
  CONCLUSIONS
  Histamine activated the IκBα/NF-κB pathway by promoting IκBα phosphorylation and inducing NF-κBp65 nuclear translocation, contributing to MUC5B overproduction and secretion.
authors.affiliation: Program in Molecular and Translational Medicine and Department of Anatomy, Schools of Medicine and Nursing Sciences, Huzhou University, Huzhou City, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Schools of Medicine and Nursing Sciences, Huzhou University, Huzhou City, China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Shao
authors.affiliation: Schools of Medicine and Nursing Sciences, Huzhou University, Huzhou City, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006632:Histamine / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055257:Mucin-5B / Q000235:genetics / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009297:Nasal Mucosa / Q000187:drug effects / Q000276:immunology*; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects / Q000276:immunology; D021381:Protein Transport / Q000187:drug effects / Q000276:immunology; D065631:Rhinitis, Allergic / Q000276:immunology*; D013450:Sulfones / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D055815:Young Adult
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D055257:Mucin-5B; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D000072000:NF-KappaB Inhibitor alpha; D006632:Histamine
keywords: MUC5B; NF-κB; inhibitor of kappa B alpha; mucin 5 subtype B; nasal epithelial cells; nuclear factor-kappa B