title: RAGE/NF-κB signaling mediates lipopolysaccharide induced acute lung injury in neonate rat model.
abstract: Lipopolysaccharide (LPS) is known to induce acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Accumulating data suggest the crucial role of RAGE in the pathogenesis of ALI/ARDS. However, the mechanism by which RAGE mediates inflammatory lung injury in the neonates remains elusive. In this study we established LPS-induced ALI model in neonate rats, and investigated the role of RAGE/NF-κB signaling in mediating ALI. We found that RAGE antibody or bortezomib reduced LPS-induced histopathological abnormalities in the lung and lung damage score. RAGE antibody or bortezomib also reduced TNF-α level in both serum and BALF of the rats. Furthermore, RAGE antibody or bortezomib significantly reduced LPS-induced upregulation of RAGE and NF-κB expression in the lung. In conclusion, we established ALI model in neonate rats to demonstrate that LPS induced inflammatory lung injury via RAGE/NF-κB signaling. Interference with RAGE/NF-κB signaling is a potential approach to prevent and treat sepsis-related ALI/ARDS.
authors.affiliation: Department of Neonatology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, PR China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Neonatal Medical Center, Huai'an Maternity and Child Healthcare Hospital, Yangzhou University Medical College Huai'an 223002, Jiangsu, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
authors.affiliation: Basic Medical Colloge, Nanjing Medical University Nanjing, Jiangsu, PR China.
authors.forename: Yian
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Neonatology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Neonatology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Neonatology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, PR China.
authors.forename: Huaipin
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Neonatology, Huai'an First People's Hospital, Nanjing Medical University 6 Beijing Road West, Huai'an 223300, Jiangsu, PR China.
authors.forename: Zhaofang
authors.initials: Z
authors.lastname: Tian
keywords: Acute lung injury; NF-κB; RAGE; bortezomib; neonate