title: Myelinated mouse nerves studied by X-ray phase contrast zoom tomography.
abstract: We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic internodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures.
authors.affiliation: Institut für Röntgenphysik, Georg-August-Universität Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany. Electronic address: mbartel@gwdg.de.
authors.forename: M
authors.initials: M
authors.lastname: Bartels
authors.affiliation: Institut für Röntgenphysik, Georg-August-Universität Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Krenkel
authors.affiliation: ESRF - The European Synchrotron, 38043 Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Cloetens
authors.affiliation: Max-Planck-Institut für Exp. Medizin, Hermann-Rein-Straße 3, 37075 Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Möbius
authors.affiliation: Institut für Röntgenphysik, Georg-August-Universität Göttingen, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany; Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), Göttingen, Germany. Electronic address: tsalditt@gwdg.de.
authors.forename: T
authors.initials: T
authors.lastname: Salditt
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D021621:Imaging, Three-Dimensional; D051379:Mice; D008810:Mice, Inbred C57BL; D008858:Microscopy, Phase-Contrast; D009186:Myelin Sheath / Q000502:physiology; D009900:Optic Nerve / Q000033:anatomy & histology / Q000648:ultrastructure*; D009993:Osmium Tetroxide / Q000494:pharmacology; D012501:Saphenous Vein / Q000033:anatomy & histology / Q000294:innervation* / Q000648:ultrastructure*; D012583:Schwann Cells / Q000166:cytology; D012584:Sciatic Nerve / Q000033:anatomy & histology / Q000648:ultrastructure*; D013194:Staining and Labeling; D014057:Tomography, X-Ray Computed
chemical_list: D009993:Osmium Tetroxide
keywords: Myelin; Nerves; Node of Ranvier; Phase contrast; X-ray tomography