title: Tagaea marinagen. nov., sp. nov., a marine bacterium isolated from shallow coastal water.
abstract: A Gram-negative, heterotrophic, marine bacterium, designated strain TT1T, was isolated from seawater collected from the shallow coastal region of Anping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolate required NaCl for growth and exhibited optimal growth at 30-35 °C, 2-4 % NaCl and pH 7-8. Strain TT1Tgrew aerobically and was not capable of anaerobic growth by fermentation of glucose or other carbohydrates. Q-10 was the sole major isoprenoid quinone. Cellular fatty acids were dominated by C18 : 1ω7c(47.5 %), C18 : 12-OH (16.3 %) and C19 : 0ω8ccyclo (10.6 %). The DNA G+C content was 56.4 mol%. Phylogeny based on 16S rRNA gene sequences showed that strain TT1Tformed a distinct genus-level lineage in the familyRhodospirillaceaeof the classAlphaproteobacteriaand exhibited the highest sequence similarity with species of the generaThalassobaculum(89.9-90.0 % 16S rRNA gene sequence similarity),Oceanibaculum(89.4-89.9 %) andNisaea(89.1-89.7 %). Strain TT1Tcould be distinguished from species of these phylogenetically closest genera based on differences in DNA G+C contents (56.4 mol% vs 60.0-68.0 mol%), fatty acid profiles and some physiological characteristics. On the basis of the polyphasic taxonomic data from this study, strain TT1Tis considered to represent a novel species of a new genus in the familyRhodospirillaceae, for which the nameTagaea marinagen. nov., sp. nov. is proposed. The type strain of the type species is TT1T( = JCM 18659T= BCRC 80493T).
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Wen
authors.initials: W
authors.lastname: Dar Jean
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Ssu-Po
authors.initials: SP
authors.lastname: Huang
authors.affiliation: College of Health Care, China Medical University, No. 91, Shyue-Shyh Rd, Taichung, Taiwan, ROC.
authors.forename: Jwo-Sheng
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Institute of Oceanography, National Taiwan University, PO Box 23-13, Taipei, Taiwan, ROC.
authors.forename: Wung Yang
authors.initials: WY
authors.lastname: Shieh