title: Interaction of green tea catechins with renal organic cation transporter 2.
abstract: 1. Green tea extract (GTE) and EGCG have previously shown to increase the uptake of MPP+into Caco-2 cells. However, whether GTE and its derivatives interact with renal basolateral organic cation transporter 2 (Oct2) which plays a crucial role for cationic clearance remains unknown. Thus, this study assessed the potential of drug-green tea (GT) catechins and its derivatives interactions with rat Oct2 using renal cortical slices and S2 stably expressing rat Oct2 (S2rOct2). 2. Both GTE and ECG inhibited MPP+uptake in renal slices in a concentration-dependent manner (IC50= 2.71 ± 0.360 mg/ml and 0.87 ± 0.151 mM), and this inhibitory effect was reversible. Inhibition of [3H]MPP+transport in S2rOct2 by either GTE or ECG (IC50= 1.90 ± 0.087 mg/ml and 1.67 ± 0.088 mM) was also observed. 3. The weak and reversible interactions of GTE and ECG with rOct2 indicate that consumption of GT beverages could not interfere with cationic drugs secreted via renal OCT2 in humans. However, the rise of therapeutic use of GTE and ECG might have to take into account the significant possibility of adverse drug-green tea catechins interactions which could alter renal organic cation drug clearance.
authors.affiliation: a Department of Physiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.|b Department of Pharmacology and Toxicology , Dokkyo Medical University, School of Medicine , Tochigi , Japan , and.
authors.forename: Chaliya
authors.initials: C
authors.lastname: Jaiyen
authors.affiliation: b Department of Pharmacology and Toxicology , Dokkyo Medical University, School of Medicine , Tochigi , Japan , and.
authors.forename: Promsuk
authors.initials: P
authors.lastname: Jutabha
authors.affiliation: b Department of Pharmacology and Toxicology , Dokkyo Medical University, School of Medicine , Tochigi , Japan , and.
authors.forename: Naohiko
authors.initials: N
authors.lastname: Anzai
authors.affiliation: a Department of Physiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Anusorn
authors.initials: A
authors.lastname: Lungkaphin
authors.affiliation: c Department of Physiology , Faculty of Science, Mahidol University , Bangkok , Thailand.
authors.forename: Sunhapas
authors.initials: S
authors.lastname: Soodvilai
authors.affiliation: a Department of Physiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Chutima
authors.initials: C
authors.lastname: Srimaroeng
keywords: Drug interaction; green tea; green tea catechins; organic cation transporter 2; renal basolateral transporter