title: A novel fluorescence aptasensor for 8-hydroxy-2'-deoxyguanosine based on the conformational switching of K(+)-stabilized G-quadruplex.
abstract: A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2'-deoxyguanosine (8-OHdG) by using 8-OHdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a K(+)-stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K(+)-stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-OHdG in the solution of Apt - K(+) ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-OHdG concentration ranging from 3.96 nM to 211 nM with a detection limit of 1.19 nM. The relative standard deviation and the recovery were 1.23-3.26% (n=11) and 94.8-106.7%, respectively. The proposed aptasensor consists of only an aptamer probe and a specific dye NMM, avoiding the complex and expensive labeling procedure. Thus it is much cheaper and more applicable.
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China; College of Public Health, Guilin Medical University, Guilin 541004, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China. Electronic address: yongsheng.w@tom.com.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Tang
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Hui-Xian
authors.initials: HX
authors.lastname: Yang
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Si-Han
authors.initials: SH
authors.lastname: Chen
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Shan-Du
authors.initials: SD
authors.lastname: Liu
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Zhu
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Wang
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Huang
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D015374:Biosensing Techniques / Q000379:methods*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D054856:G-Quadruplexes; D011188:Potassium / Q000737:chemistry*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D011188:Potassium
keywords: 8-Hydroxy-2′-deoxyguanosine; Conformational switching; Fluorescence aptasensor; K(+)-stabilized G-quadruplex; N-methyl mesoporphyrin IX