title: Azole Resistance in Aspergillus fumigatus Clinical Isolates from an Italian Culture Collection.
abstract: The aims of the study were to investigate the prevalence of azole resistance among Aspergillus fumigatus clinical isolates. A total of 533 clinical isolates that had been collected between 1995 and 2006, from 441 patients, were screened. No resistance was detected in isolates collected between 1995 and 1997. Starting in 1998, the resistance rate was 6.9%; a total of 24 patients (6.25%) harbored a resistant isolate. The TR34/L98H substitution was found in 21 of 30 tested isolates.
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lazzarini
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Maria Carmela
authors.initials: MC
authors.lastname: Esposto
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Prigitano
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Cogliati
authors.affiliation: Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy, Università degli Studi di Milano, Milan, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: De Lorenzis
authors.affiliation: Department of Biomedical Sciences for Health, Università degli Studi di Milano, Milan, Italy annamaria.tortorano@unimi.it.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Tortorano
mesh_terms: D019943:Amino Acid Substitution; D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D025141:Drug Resistance, Fungal / Q000235:genetics*; D062665:Epidemiological Monitoring; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018895:Microsatellite Repeats; D009154:Mutation; D011401:Promoter Regions, Genetic; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D014230:Triazoles; D017964:Itraconazole; C101425:posaconazole; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus; D065819:Voriconazole