title: Optimization of Liquid Fermentation Medium for Production of Inonotus sanghuang (Higher Basidiomycetes) Mycelia and Evaluation of their Mycochemical Contents and Antioxidant Activities.
abstract: Inonotus sanghuang, an authentic "Sanghuang" mushroom used in traditional Chinese medicine, is known to possess important pharmacological activities. In this study, we aimed to optimize the liquid fermentation medium for I. sanghuang mycelial production and to determine the effects of two-stage cultivation (shake and static) on the yield of total flavonoids, total phenolics, and polysaccharides, as well as the antioxidant activities of I. sanghuang mycelial extracts (ISME). Under an optimized medium composition (38.96 g/L of corn flour, 4.15 g/L of yeast extract, 20.55 g/L of bran and pH 6.39), the predicted and experimental optimal mycelial biomasses were 17.60 g/L and 18.33±0.86 g/L, respectively. The results of two-stage cultivation showed that contents of total flavonoids and total phenolics in mycelia increased by 37.92% and 77.27%, respectively. However, irregular polysaccharide contents were noted throughout the experimental period. Antioxidant assays showed that ISME possessed good free-radical scavenging activity, which is mainly contributed by polyphenolic-type metabolites.
authors.affiliation: Institute of Microbiology, P.O. Box 61, Beijing Forestry University, Beijing 100083, China; Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Tian
authors.affiliation: Institute of Microbiology, P.O. Box 61, Beijing Forestry University, Beijing 100083; and Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Dai
authors.affiliation: Shandong Province Key Laboratory of Applied Mycology, School of Life Sciences, Qingdao Agricultural University, Qingdao, People's Republic of China.
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Song
authors.affiliation: Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Lean Teik
authors.initials: LT
authors.lastname: Ng
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development*; D002681:China; D003470:Culture Media; D005285:Fermentation; D025282:Mycelium / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003470:Culture Media