title: Screening anaphylactic components of MaiLuoNing injection by using rat basophilic leukemia-2H3 cell membrane chromatography coupled with HPLC-ESI-TOF-MS.
abstract: MaiLuoNing injection is a traditional Chinese medicine that used clinically since the 1950s in China. However, anaphylactic reactions, through the potentiation of mast cell degranulation, have been reported. In the present study, a rat basophilic leukemia-2H3 cell membrane chromatography coupled with high-performance liquid chromatography and electrospray ionization-ion trap-time of flight-mass spectrometry method was established for screening, analyzing, and identifying the potential anaphylactic components of MaiLuoNing injection. Harpagoside, a potential degranulator of rat basophilic leukemia-2H3 cells, was retained in rat basophilic leukemia-2H3 cell membrane chromatography. We aimed to evaluate the retained components to determine which of those were capable of inducing degranulation of basophilic leukemia cells. A β-hexosaminidase assay revealed that harpagoside can induce rat basophilic leukemia-2H3 cell degranulation in a dose-dependent manner. BLBA/c mice also exhibit passive cutaneous anaphylaxis in response to harpagoside. These results indicate that rat basophilic leukemia-2H3 cell membrane chromatography coupled with high-performance liquid chromatography and electrospray ionization ion trap time-of-flight mass spectrometry is effective in screening for the anaphylactic components of MaiLuoNing injection.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Han
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Lv
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Xue
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.|National-Provincial Joint Engineering Research Center for Natural Vascular Medicine Screening & Analysis, Xi'an, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D015471:Leukemia, Basophilic, Acute / Q000473:pathology*; D051381:Rats; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C534919:mailuoning
keywords: Anaphylactic components; Cell membrane chromatography, Harpagoside, Ion trap mass spectrometry, MaiLuoNing injection