title: Dose-dependent effects of isoflavone exposure during early lifetime on development and androgen sensitivity in male Wistar rats.
abstract: METHODS
  The aim of our study was to investigate dose-dependent effects of isoflavone (ISO) exposure during adolescence on the androgen sensitivity of various physiological end points in male Wistar rats.
  
  
  RESULTS
  During embryogenesis and adolescence, rats were exposed to an ISO-depleted diet (IDD) or one of two diets enriched with different concentrations of a soy-based ISO extract causing plasma concentrations observed averagely (ISO-rich diet [IRD]low) and maximally (IRDhigh) in Asian men. Most of the rats were orchiectomized at postnatal day (PND) 81 and were treated with testosterone propionate (TP) or vehicle from PND 89 to 99. In intact rats (PND 99) body weight, food intake, and fat mass were not influenced by ISO, but serum triglycerides and hepatic fatty acid synthase expression were decreased. Trabecular bone mineral density (BMD) was reduced in IRDlow, but not in IRDhigh rats. Orchiectomy (ORX) induced loss of BMD, which was antagonized by IRDhigh. ISO increased androgen sensitivity of seminal vesicle and levator ani. Besides, ISO plasma levels were reduced by ORX compared to intact and TP-treated rats.
  
  
  CONCLUSIONS
  In summary, the results of this study indicate that exposure to ISO during adolescence affects bone homeostasis, lipid metabolism, and modulates androgen sensitivity in young adult male rats.
authors.affiliation: Department of Molecular and Cellular Sports Medicine, Institute of Cardiovascular Research and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Müller
authors.affiliation: Department of Molecular and Cellular Sports Medicine, Institute of Cardiovascular Research and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Basso
authors.affiliation: Department of Molecular and Cellular Sports Medicine, Institute of Cardiovascular Research and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Kurrat
authors.affiliation: Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut (MRI), Karlsruhe, Germany.
authors.forename: Sebastian T
authors.initials: ST
authors.lastname: Soukup
authors.affiliation: Institute of Biomechanics and Orthopaedics, German Sport University, Cologne, Germany.|Cologne Center for Musculoskeletal Biomechanics, Medical Faculty, University of Cologne, Cologne, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Niehoff
authors.affiliation: Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut (MRI), Karlsruhe, Germany.
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Kulling
authors.affiliation: Department of Molecular and Cellular Sports Medicine, Institute of Cardiovascular Research and Sports Medicine, German Sport University, Cologne, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Diel
mesh_terms: D000728:Androgens / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D001823:Body Composition / Q000187:drug effects*; D015519:Bone Density / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007529:Isoflavones / Q000097:blood / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood; D043343:Testosterone Propionate / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood
chemical_list: D000728:Androgens; D007529:Isoflavones; D014280:Triglycerides; D013739:Testosterone; D043343:Testosterone Propionate
keywords: Androgen sensitivity; Bone; Lipid metabolism; Male; Soy isoflavones