title: Shifts in rDNA levels act as a genome buffer promoting chromosome homeostasis.
abstract: The nucleolus is considered to be a stress sensor and rDNA-based regulation of cellular senescence and longevity has been proposed. However, the role of rDNA in the maintenance of genome integrity has not been investigated in detail. Using genomically diverse industrial yeasts as a model and array-based comparative genomic hybridization (aCGH), we show that chromosome level may be balanced during passages and as a response to alcohol stress that may be associated with changes in rDNA pools. Generation- and ethanol-mediated changes in genes responsible for protein and DNA/RNA metabolism were revealed using next-generation sequencing. Links between redox homeostasis, DNA stability, and telomere and nucleolus states were also established. These results suggest that yeast genome is dynamic and chromosome homeostasis may be controlled by rDNA.
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Deregowska
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Adamczyk
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kwiatkowska
authors.affiliation: b Department of Genomics and Molecular Biology of Animals ; Laboratory of Genomics; National Research Institute of Animal Production ; Cracow , Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Gurgul
authors.affiliation: c Department of Genetics ; Institute of Biochemistry and Biophysics; Polish Academy of Sciences ; Warsaw , Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Skoneczny
authors.affiliation: d Laboratory of Mutagenesis and DNA Repair; Institute of Biochemistry and Biophysics; Polish Academy of Sciences ; Warsaw , Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Skoneczna
authors.affiliation: b Department of Genomics and Molecular Biology of Animals ; Laboratory of Genomics; National Research Institute of Animal Production ; Cracow , Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Szmatola
authors.affiliation: b Department of Genomics and Molecular Biology of Animals ; Laboratory of Genomics; National Research Institute of Animal Production ; Cracow , Poland.
authors.forename: Igor
authors.initials: I
authors.lastname: Jasielczuk
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Magda
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Rawska
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Pabian
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Anita
authors.initials: A
authors.lastname: Panek
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kaplan
authors.affiliation: e Department of Biochemistry and Cell Biology ; University of Rzeszow; Rzeszow , Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: a Department of Genetics ; University of Rzeszow ; Rzeszow , Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D002466:Cell Nucleolus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015825:Chromosomes, Fungal / Q000235:genetics* / Q000378:metabolism; D055028:Comparative Genomic Hybridization; D004249:DNA Damage; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D042822:Genomic Instability; D006706:Homeostasis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012641:Selection, Genetic; D016615:Telomere / Q000235:genetics / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D000431:Ethanol
keywords: chromosomes; ethanol; genome profiling; industrial yeasts; rDNA; selective pressure