title: Brevibacillus sediminissp. nov., isolated from a hot spring.
abstract: Strain YIM 78300T, a novel Gram-stain-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacterium, was recovered from the sediment of a hot spring in the Tagejia Geothermal Field, Angren, Tibet province, western China. Optimum growth was observed at 50-55 °C, at pH 7.0 and with 0-1.5 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence of strain YIM 78300Tindicated that it belongs to the genusBrevibacillus. Similarity levels between the 16S rRNA gene sequences of the new isolate and those of the type strains ofBrevibacillusmembers were 96.9-96.3 %; highest sequence similarity was withBrevibacillus thermoruberDSM 7064T. The predominant menaquinone was MK-7 and the major cellular fatty acids were iso-C15 : 0and iso-C17 : 0. The major polar lipids were phosphatidyl-N-methylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified phospholipids, an unidentified aminophospholipid and two unidentified polar lipids. The G+C content of the genomic DNA of strain YIM 78300Twas 57.9 mol%. Based on phylogenetic analyses, and physiological and biochemical characteristics, strain YIM 78300Tis considered to represent a novel species of the genusBrevibacillus, for which the nameBrevibacillus sediminissp. nov. is proposed. The type strain is YIM 78300T( = DSM 29928T= CPCC 100738T).
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PRChina.
authors.forename: Lan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Chang-Guo
authors.initials: CG
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Firasat
authors.initials: F
authors.lastname: Hussain
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Inamullah
authors.initials: I
authors.lastname: Khan
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Neeli
authors.initials: N
authors.lastname: Habib
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PRChina.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou 510275, PRChina.|Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PRChina.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li