title: MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.
abstract: Phagocytosis is a critical cellular process for innate immune defense against microbial infection. The regulation of phagocytosis process is complex and has not been well defined. An intracellular molecule might regulate cell surface-initiated phagocytosis, but the underlying molecular mechanism is poorly understood (1). In this study, we found that microtubule-associated protein 1S (MAP1S), a protein identified recently that is involved in autophagy (2), is expressed primarily in macrophages. MAP1S-deficient macrophages are impaired in the phagocytosis of bacteria. Furthermore, we demonstrate that MAP1S interacts directly with MyD88, a key adaptor of Toll-like receptors (TLRs), upon TLR activation and affects the TLR signaling pathway. Intriguingly, we also observe that, upon TLR activation, MyD88 participates in autophagy processing in a MAP1S-dependent manner by co-localizing with MAP1 light chain 3 (MAP1-LC3 or LC3). Therefore, we reveal that an intracellular autophagy-related molecule of MAP1S controls bacterial phagocytosis through TLR signaling.
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, Texas 77030 and.
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: the School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Leyuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, Texas 77030 and.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and.
authors.forename: Joseph
authors.initials: J
authors.lastname: Cleveland
authors.affiliation: the School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.affiliation: the School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Wallace L
authors.initials: WL
authors.lastname: McKeehan
authors.affiliation: Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology, Texas A&M University Health Science Center, Houston, Texas 77030 and liyugene@hit.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.affiliation: From the Center for Infectious and Inflammatory Diseases and dzhang@ibt.tamhsc.edu.
authors.forename: Dekai
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D002478:Cells, Cultured; D057809:HEK293 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D053594:Myeloid Differentiation Factor 88 / Q000819:agonists* / Q000378:metabolism; D010587:Phagocytosis; D021381:Protein Transport; D000067996:RAW 264.7 Cells; D012486:Salmonella typhimurium / Q000276:immunology*; D015398:Signal Transduction; D013047:Specific Pathogen-Free Organisms; D013211:Staphylococcus aureus / Q000276:immunology*; D051193:Toll-Like Receptors / Q000819:agonists / Q000378:metabolism
chemical_list: C570460:MAP1S protein, mouse; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D051193:Toll-Like Receptors
keywords: Toll-like receptor (TLR); autophagy; bacterial adhesion; innate immunity; phagocytosis