title: Design and testing of an assessment instrument to measure understanding of protein structure and enzyme inhibition in a new context.
abstract: Assessment instruments designed to measure student conceptual understanding and skills proficiency related to biochemistry are important to transform undergraduate biochemistry education. The purpose of this study was to develop an assessment instrument to measure student understanding of protein structure and enzyme inhibition in a new context, that of saturable binding. A community of biochemistry educators was involved in an iterative process of designing and testing of this assessment, which consists of true/false and open-ended questions that map to low and high levels in Bloom's taxonomy. A total of 188 students' responses were collected from seven different institutions and were graded by two independent raters using a rubric. Results from this administration indicate that most students were able to answer the questions related to lower-levels in Bloom's taxonomy; however for higher-level questions, students had more difficulty. The results from this assessment can give instructors insight into the ways in which persistent incorrect or incomplete ideas related to protein structure and binding events can hinder knowledge application. Also, the use of a community of practice to develop assessment instruments of this type and the format of the instrument itself could be a useful model for development of assessment instruments in the future.
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, Florida, 33620.
authors.forename: Sachel M
authors.initials: SM
authors.lastname: Villafañe
authors.affiliation: Department of Chemistry, Tabor College, Hillsboro, Kansas, 67063.
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Heyen
authors.affiliation: Department of Chemistry, University of South Florida, Tampa, Florida, 33620.|Center for the Improvement of Teaching and Research in Undergraduate STEM Education, University of South Florida, Tampa, Florida, 33620.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Lewis
authors.affiliation: Department of Chemistry, Seattle University, Seattle, Washington, 98122.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Loertscher
authors.affiliation: Department of Chemistry, Seattle University, Seattle, Washington, 98122.
authors.forename: Vicky
authors.initials: V
authors.lastname: Minderhout
authors.affiliation: Department of Chemistry and Biochemistry, Capital University, Columbus, Ohio, 43209.
authors.forename: Tracey Arnold
authors.initials: TA
authors.lastname: Murray
mesh_terms: D032882:Comprehension; D004521:Educational Measurement / Q000379:methods* / Q000592:standards*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018794:Problem-Based Learning; D011487:Protein Conformation; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D013334:Students / Q000523:psychology*
chemical_list: D004791:Enzyme Inhibitors; D011506:Proteins
keywords: assessment and the design of probes for student understanding and learning; assessment of educational activities; protein structure function and action mechanism