title: Metabolomic Analysis of Plasma From Patients With Acute Mountain Sickness Using Chromatography-Mass Spectrometry.
abstract: Although acute mountain sickness (AMS) has long been recognized, little is known about this condition to date. The current study was conducted to explore changes in the metabolomic profiles of AMS patients and to further assess the potential of using these changes for the diagnosis of AMS. Plasma samples from 12 patients with AMS and 12 individuals without AMS were collected and used for further bioinformatics analysis by gas chromatography-mass spectrometry (GC-MS). The following analytical methods were used: gas chromatography-mass spectrometry data preprocessing, principal components analysis, partial least squares-discriminant analysis, model validation, orthogonal partial least squares-discriminant analysis, and the screening and identification of differences in metabolites. The results revealed a significantly difference between the subjects with AMS and those in the control group. Compared with plasma from the controls, plasma from the AMS patients contained signi&filig;cantly increased hypoxanthine, cysteinylglycine, D-arabitol, L-allothreonine, 2-ketobutyric acid, and succinate semialdehyde. The identification of metabolomic changes may be useful for the diagnosis of AMS in the future and may lay the foundation for further study of AMS pathogenesis.
authors.affiliation: From the Department of Health Management, Xinqiao Hospital (GZ); and Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, China (CY, ZT, TW, SW, QX, GG).
authors.forename: Guoyan
authors.initials: G
authors.lastname: Zhu
authors.forename: Changlin
authors.initials: C
authors.lastname: Yin
authors.forename: Zhu
authors.initials: Z
authors.lastname: Tian
authors.forename: Tinggang
authors.initials: T
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xiang
authors.forename: Guoning
authors.initials: G
authors.lastname: Guo
mesh_terms: D000328:Adult; D000532:Altitude Sickness / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D055442:Metabolome; D055815:Young Adult