title: The antioxidant system of seminal fluid during in vitro storage of sterlet Acipenser ruthenus sperm.
abstract: The role of the seminal fluid antioxidant system in protection against damage to spermatozoa during in vitro sperm storage is unclear. This study investigated the effect of in vitro storage of sterlet Acipenser ruthenus spermatozoa together with seminal fluid for 36 h at 4 °C on spermatozoon motility rate and curvilinear velocity, thiobarbituric acid reactive substance level, and components of enzyme and non-enzyme antioxidant system (superoxide dismutase and catalase activity and uric acid concentration) in seminal fluid. Spermatozoon motility parameters after sperm storage were significantly decreased, while the level of thiobarbituric acid reactive substances, activity of superoxide dismutase and catalase, and uric acid concentration did not change. Our findings suggest that the antioxidant system of sterlet seminal fluid is effective in preventing oxidative stress during short-term sperm storage and prompt future investigations of changes in spermatozoon homeostasis and in spermatozoon plasma membrane structure which are other possible reasons of spermatozoon motility deterioration upon sperm storage.
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic. vdzyuba@frov.jcu.cz.|Institute of Biology, V. N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkiv, 61022, Ukraine. vdzyuba@frov.jcu.cz.
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Dzyuba
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
authors.forename: Jacky
authors.initials: J
authors.lastname: Cosson
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
authors.forename: Borys
authors.initials: B
authors.lastname: Dzyuba
authors.affiliation: Aquaculture Department, Faculty of Fisheries, Istanbul University, Beyazit/Eminonu, 34452, Istanbul, Turkey.
authors.forename: Gunes
authors.initials: G
authors.lastname: Yamaner
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Rodina
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic.
authors.forename: Otomar
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D005399:Fishes / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress; D012661:Semen / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014527:Uric Acid
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D014527:Uric Acid; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Antioxidant system; In vitro storage; Motility; Seminal fluid; Sterlet sperm