title: All-Trans Retinoic Acid Induces Expression of a Novel Intergenic Long Noncoding RNA in Adult rat Primary Hippocampal Neurons.
abstract: Around 90% of the mammalian genome undergoes pervasive transcription into various types of small and long regulatory noncoding RNAs, whereas only ∼ 1.5% codes for proteins. Long noncoding RNAs (lncRNAs) constitute diverse classes of sense- and antisense transcripts that are abundantly expressed in the mammalian central nervous system (CNS) in cell type- and developmental stage-specific manners. They are implicated in brain development, differentiation, neuronal plasticity, and other cognitive functions. Mammalian brain requires the vitamin A metabolite all-trans retinoic acid (atRA) for its normal development, differentiation, and cell-fate determination. However, its role in adult brain function is less understood. Here, we report atRA-mediated transcriptional upregulation of endogenous expression of a novel long intergenic noncoding RNA-rat brain expressed (LINC-RBE) in cultured primary hippocampal neurons from adult rat. We have previously reported LINC-RBE as an intergenic, simple repeat sequence containing lncRNA highly expressed in the rat brain. This is a first-time report of involvement of atRA in transcriptional upregulation of lncRNA expression in rat hippocampal neurons. Therefore, it may be involved in regulation of brain function and disease.
authors.affiliation: Molecular Biology Laboratory, School of life Sciences, Jawaharlal Nehru University, New Delhi, -110067, India.
authors.forename: Sukhleen
authors.initials: S
authors.lastname: Kour
authors.affiliation: Molecular Biology Laboratory, School of life Sciences, Jawaharlal Nehru University, New Delhi, -110067, India. pcrath@mail.jnu.ac.in.
authors.forename: Pramod C
authors.initials: PC
authors.lastname: Rath
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D014212:Tretinoin / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding; D014212:Tretinoin
keywords: Actinomycin D; Fluorescence in-situ RNA hybridization; Long noncoding RNA; Primary hippocampal neurons; Retinoic acid