title: Growth inhibition and possible mechanism of oleamide against the toxin-producing cyanobacterium Microcystis aeruginosa NIES-843.
abstract: Oleamide, a fatty acid derivative, shows inhibitory effect against the bloom-forming cyanobacterium Microcystis aeruginosa. The EC50 of oleamide on the growth of M. aeruginosa NIES-843 was 8.60 ± 1.20 mg/L. In order to elucidate the possible mechanism of toxicity of oleamide against M. aeruginosa, chlorophyll fluorescence transient, cellular ultrastructure, fatty acids composition and the transcription of the mcyB gene involved in microcystins synthesis were studied. The results of chlorophyll fluorescence transient showed that oleamide could destruct the electron accepting side of the photosystem II of M. aeruginosa NIES-843. Cellular ultrastructure examination indicated that the destruction of fatty acid constituents, the distortion of thylakoid membrane and the loss of integrity of cell membrane were associated with oleamide treatment and concentration. The damage of cellular membrane increased the release of microcystins from intact cells into the medium. Results presented in this study provide new information on the possible mechanisms involved and potential utilization of oleamide as an algicide in cyanobacterial bloom control.
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Jihai
authors.initials: J
authors.lastname: Shao
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: He
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Chen
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
authors.forename: Si
authors.initials: S
authors.lastname: Luo
authors.affiliation: College of Resources and Environment, Hunan Agricultural University, Changsha, 410128, People's Republic of China. jdgu@hku.hk.|Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Agricultural University, Changsha, 410128, People's Republic of China. jdgu@hku.hk.|Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, People's Republic of China. jdgu@hku.hk.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Gu
mesh_terms: D002462:Cell Membrane / Q000187:drug effects; D052998:Microcystins / Q000378:metabolism*; D046931:Microcystis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D009829:Oleic Acids / Q000494:pharmacology*; D010571:Pest Control; D045332:Photosystem II Protein Complex / Q000378:metabolism; D020524:Thylakoids / Q000187:drug effects
chemical_list: D052998:Microcystins; D009829:Oleic Acids; D045332:Photosystem II Protein Complex; C029407:oleylamide
keywords: Growth inhibition; Microcystins; Microcystis aeruginosa; Oleamide; Photosynthesis