title: The effect of kinin B1 receptor on chronic itching sensitization.
abstract: BACKGROUND
  Altered kallikrein-related peptidase activity and bradykinin are associated with skin disorders in humans and mice under chronic inflammation conditions. The bradykinin B1 receptor (B1R), also known as one of the G-protein-coupled receptor family and usually absent in intact tissues and upregulated during tissue injury, is responsible for vasodilation, capillary permeability, nociceptor sensitization, and pain; it is indispensable for physiopathological progress in chronic inflammation conditions, but its roles and effectors in the itching sensation of the allergic contact dermatitis model are poorly defined.
  
  
  RESULTS
  We focused on incurable itching in a diphenylcyclopropenone (DCP) chronic inflammation experimental model. Preventive treatment with the B1R antagonist R892 significantly suppressed spontaneous scratching, while the B2R selective antagonist did not. B1R expression in the skin tissues of this model was detected using a quantitative, real-time polymerase chain reaction, Western blotting, and immunohistochemistry; B1R mRNA and protein levels were increased compared with a sham-treated control group. A higher B1R IHC staining signal was observed in the keratinocytes in DCP-treated mice compared with a vehicle-treated group, so we studied the B1R function when superimposed on a protease-activated receptor 2 (PAR2) background, establishing B1R as a pivotal mediator of PAR2 function in HaCaT cell lines.
  
  
  CONCLUSIONS
  Our data provide evidence that B1R facilitates the chronic itching sensation related to keratinocytes in a DCP-treated chronic inflammation experimental model.
authors.affiliation: Postgraduate Institute, Southern Medical University, Guangzhou, 510015, People's Republic of China. dr.liuyuy@outlook.com.|Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChuan Road, Guangzhou, 510080, People's Republic of China. dr.liuyuy@outlook.com.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Postgraduate Institute, Southern Medical University, Guangzhou, 510015, People's Republic of China. 490250958@qq.com.|Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChuan Road, Guangzhou, 510080, People's Republic of China. 490250958@qq.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Postgraduate Institute, Southern Medical University, Guangzhou, 510015, People's Republic of China. 347283640@qq.com.|Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChun Road, Guangzhou, 510080, People's Republic of China. 347283640@qq.com.
authors.forename: Mengran
authors.initials: M
authors.lastname: Li
authors.affiliation: Postgraduate Institute, Southern Medical University, Guangzhou, 510015, People's Republic of China. 1349687184@qq.com.|Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChun Road, Guangzhou, 510080, People's Republic of China. 1349687184@qq.com.
authors.forename: Sailin
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChun Road, Guangzhou, 510080, People's Republic of China. lijessy@msn.com.
authors.forename: Jiexian
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Anesthesiology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, 96 DongChuan Road, Guangzhou, 510080, People's Republic of China. jiwenjin@msn.com.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Ji
mesh_terms: D000818:Animals; D000982:Antipruritics / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D001920:Bradykinin / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D003521:Cyclopropanes / Q000494:pharmacology; D017449:Dermatitis, Allergic Contact / Q000503:physiopathology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011537:Pruritus / Q000139:chemically induced / Q000503:physiopathology*; D043783:Receptor, Bradykinin B1 / Q000235:genetics / Q000378:metabolism*; D012677:Sensation / Q000187:drug effects*
chemical_list: D000982:Antipruritics; D003521:Cyclopropanes; D043783:Receptor, Bradykinin B1; C065679:icatibant; C029402:diphenylcyclopropenone; D001920:Bradykinin