title: Allopregnanolone attenuates Aβ25-35-induced neurotoxicity in PC12 cells by reducing oxidative stress.
abstract: Massive accumulation of amyloid beta (Aβ) has been implicated as a pivotal event in the pathogenesis of Alzheimer's disease. The underlying mechanisms of Aβ-induced neurotoxicity include generation of reactive oxidative species (ROS), inflammation, and neurons loss. Allopregnano-lone (APα), a neurosteroid derive from neuroactive progesterone, has been demonstrated to have neuroprotective properties in vivo and vitro. In the present study, the effects of APα on oxidative damage in Aβ25-35-treated pheochromocytoma (PC12) cells were investigated. Pretreatment of APα significantly attenuated Aβ25-35-induced neuronal death. APα decreased the intracellular ROS generation and reduced lipid peroxidation induced by Aβ25-35. In addition, APα treatment enhanced antioxidant enzyme superoxide dismutase (SOD) activity. This study demonstrates that APα exerts a protective effect against Aβ25-35-induced neurotoxicity in PC12 cells. The protective role of APα likely results from inhibition of oxidative stress.
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qian
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Qinsai
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Peishun
authors.initials: P
authors.lastname: Qin
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ji
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Kaiming
authors.initials: K
authors.lastname: Yuan
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Fanghua
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Xuhua
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Lian
authors.affiliation: Department of Anesthesiology, Critical Care and Pain Medicine, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
keywords: Allopregnanolone; PC12 cells; amyloid beta; reactive oxidative species