title: Willed-movement training reduces brain damage and enhances synaptic plasticity related proteins synthesis after focal ischemia.
abstract: It has been wildly accepted that willed movement(WM) training promotes neurological rehabilitation in patients with stroke. However, it was not clear whether the effect of WM is better than other forms of exercise. The purpose of this study is to assess different effects of WM and other forms of exercise on rats with focal ischemia. The subjects are all had right middle cerebral artery occlusion (MCAO) surgery and randomly allocated to three groups of training and one control group with no training. Infarct volume by 2,3,5-triphenyltetrazolium chloride (TTC) dye, expression of PICK1 and synaptophysin in cerebral cortex and striatum of injured side by western blotting and immunofluorescence performed are analyzed. Exercise has done respectively on rats in each group for 15 days and 30 days. Compared with the control group, the brain damage is reduced in other groups after 15 days exercise. The protein expressions levels of synaptophysin and PICK1 are upregulated after exercise. Concentration of PICK1 protein in WM is greater than other exercise groups, and the expression of synaptophysin in WM and SM groups are higher than EM groups. The number of PICK1 positive cells, synaptophysin and PICK1 co-positive cells are increased by exercise. Synaptophysin is widely distributed in cortex surrounding the injury area in WM and EM. It is indicated in our result that willed-movement training is the most effective intervention in enhancing the PICK1-mediated synaptic plasticity in the area adjacent to the damage region of ischemic rats.
authors.affiliation: Department of Neurology, Xiang Ya hospital, Central South University, Xiang Ya Road 87, 410008 Changsha, Hunan, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Nie
authors.affiliation: Department of Neurology, Xiang Ya hospital, Central South University, Xiang Ya Road 87, 410008 Changsha, Hunan, China. Electronic address: sjnk_xy@aliyun.com.
authors.forename: Xiaosu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurology, Brain Hospital of Hunan Province, Hunan University of Chinese Medicine, Fu Rong Road 427, Changsha 410007 Hunan, China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Radiology, The Second Xiangya hospital of Central South University, People Road 139, 410011 Changsha, Hunan, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Department of research and education office, XiangYa Stomatological Hospital, Central South University, Xiang Ya Road 72, 410008 Changsha, Hunan, China.
authors.forename: Simin
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000473:pathology* / Q000503:physiopathology*; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology / Q000534:rehabilitation*; D002352:Carrier Proteins / Q000378:metabolism; D003598:Cytoskeletal Proteins; D004195:Disease Models, Animal; D005081:Exercise Therapy / Q000379:methods*; D005455:Fluorescent Antibody Technique; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009068:Movement / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D020521:Stroke / Q000473:pathology / Q000503:physiopathology; D000071939:Stroke Rehabilitation; D013569:Synapses / Q000473:pathology / Q000502:physiology; D016896:Treatment Outcome
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D009687:Nuclear Proteins; C498298:PICK1 protein, rat
keywords: PICK1; Rehabilitation; Stroke; Synaptic plasticity; Synaptophysin; Willed movement training