title: Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section.
abstract: Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10-20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 481-488, 2017.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Hasegawa
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Haraguchi
authors.affiliation: Panasonic Corporation, Osaka, Japan.
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Oikaze
authors.affiliation: Panasonic Corporation, Osaka, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kabetani
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.|School of Creative Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan.
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Sakaguchi
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D002417:Cattle; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D041681:NIH 3T3 Cells; D023822:Tissue Engineering; D041623:Tomography, Optical Coherence
keywords: cell migration; cell sheet; cross-sectional observation; high-resolution; optical coherence microscopy