title: Molecular detection of Ehrlichia canis, Hepatozoon canis and Babesia canis vogeli in stray dogs in Mahasarakham province, Thailand.
abstract: Canine tick borne diseases showing distribution worldwide have caused morbidity and mortality in dogs. This study observed the mainly tick borne pathogens described for dogs in Thailand, Ehrlichia canis, Hepatozoon canis and Babesia canis vogeli. From May to July 2014, blood samples were collected from 79 stray dogs from 7 districts of Mahasarakham province to molecular surveyed for 16s rRNA gene of E. canis and 18s rRNA gene of H. canis and B. canis vogeli. Twenty eight (35.44%) of stray dogs showed the infection with tick borne pathogens. The prevalence of E. canis infection was the highest with 21.5% (17/79). DNA of H. canis and B. canis vogeli were detected at the prevalence of 10.1% (8/79) and 6.3% (5/79), respectively. Co-infection between E. canis and B. canis vogeli were identified in 2 (2.5%) dogs. The results indicated that a wide range of tick borne pathogens are circulation in the canine population in Mahasarakham province. This study is the first report on prevalence of E. canis, H. canis and B. canis vogeli in stray dogs in Mahasarakham, a province in northern part of Thailand. This data providing is important to understand the prevalence of E. canis, H. canis and B. canis vogeli infection in stray dogs in this region, which will assist in the management of these blood parasite.
authors.affiliation: Department of Veterinary Public Health; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham, 44000, Thailand.
authors.forename: Supawadee
authors.initials: S
authors.lastname: Piratae
authors.affiliation: Department of Veterinary Pre-Clinic; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham, 44000, Thailandc.
authors.forename: Kiattisak
authors.initials: K
authors.lastname: Pimpjong
authors.affiliation: Veterinary Teaching Hospital; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham, 44000, Thailand.
authors.forename: Kotchaphon
authors.initials: K
authors.lastname: Vaisusuk
authors.affiliation: Department of Veterinary Clinic; Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham, 44000, Thailand.
authors.forename: Wasupon
authors.initials: W
authors.lastname: Chatan
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000453:epidemiology*; D003048:Coccidiosis / Q000453:epidemiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D041061:Ehrlichia canis / Q000235:genetics / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000453:epidemiology / Q000662:veterinary*; D016783:Eucoccidiida / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005843:Geography; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013785:Thailand / Q000453:epidemiology; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal