title: Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes.
abstract: To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model.
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Manabe
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Ogino
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ito
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Furuichi
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Takagi
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan.
authors.forename: Mio
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan; Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Goto-Inoue
authors.affiliation: Department of Stem Cell Biology, Atomic Bomb Disease Institute, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ono
authors.affiliation: Department of Health Promotion Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Hachioji 192-0397, Japan. Electronic address: fujiin@tmu.ac.jp.
authors.forename: Nobuharu L
authors.initials: NL
authors.lastname: Fujii
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D003576:Electron Transport Complex IV / Q000032:analysis; D051379:Mice; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D009211:Myoglobin / Q000032:analysis; D018995:Myosin Heavy Chains / Q000032:analysis; D012518:Sarcomeres / Q000648:ultrastructure
chemical_list: D009211:Myoglobin; D003576:Electron Transport Complex IV; D018995:Myosin Heavy Chains
keywords: Contraction; Myotubes; Primary cultured cells; Skeletal muscle