title: Dynamic Change of CD34 Level during the Survival Process of Narrow Pedicle Flap.
abstract: OBJECTIVE
  To evaluate the dynamic change of CD34 level during the survival process of narrow pedicle flaps.
  
  
  METHODS
  Twenty-five white pigs were randomly and equally divided into 5 experimental groups. Five different type of narrow pedicle with different length-to-width ratio were employed, and each type of narrow pedicle was covered with 5 different size random flaps and which was classified into A, B, C, D and E for 5 groups. Group A was control group. Each type narrow pedicle with 5 different skin flaps were implanted onto the back of the pigs along the midline of back with a reverse direction. A 0.3 cm×0.3 cm full thickness skin flap in the middle of distal segment was collected and on 3rd, 5th, 7th and 14th days of post-operation. The expression of CD34 was measured by immunohistochemistry and enzyme-linked immunosorbent (ELISA).
  
  
  RESULTS
  Histological examination showed that with the increasing of length-to-width ratio of the narrow pedicle skin flaps, the expression of CD34 increased in the skin flaps. Increased level of CD34 was found on 3rd day post-operation, and the peak expression was found on 7th day. Persistent high level of CD34 was found until 14th day.
  
  
  CONCLUSIONS
  Increased CD34 level in the distal skin flap, there is the association between CD34 level and ischemia injury. Moreover, CD34 expression plays an important role during the repair processes of pedicle flaps.
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Tianlan
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Daojiang
authors.initials: D
authors.lastname: Yu
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Han
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Yu
authors.affiliation: Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D006085:Graft Survival / Q000502:physiology*; D011184:Postoperative Period; D012867:Skin / Q000378:metabolism*; D013524:Surgical Flaps; D013552:Swine
chemical_list: D018952:Antigens, CD34