title: Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover.
abstract: The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.
authors.affiliation: Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Yueh-Fen
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Nelson
authors.affiliation: Department of Statistics, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Po-Hsu
authors.initials: PH
authors.lastname: Chen
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Joerg
authors.initials: J
authors.lastname: Graf
authors.affiliation: Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA; Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave, Wooster, OH 44691, USA.
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA; Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA. Electronic address: yu.226@osu.edu.
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D056804:Biofuels / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology*; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000096:biosynthesis; D064307:Microbiota; D010802:Phylogeny; D025341:Principal Component Analysis; D012037:Refuse Disposal / Q000295:instrumentation* / Q000379:methods*; D017422:Sequence Analysis, DNA; D014865:Waste Disposal, Fluid; D014866:Waste Products; D003313:Zea mays / Q000737:chemistry*
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D014866:Waste Products; D008697:Methane
keywords: 16S rRNA gene; Illumina sequencing; Metagenomics; Microbiome; Succession