title: Importance of duration and number of ischemic postconditioning cycles in preventing reperfusion mesenteric injuries. Experimental study in rats.
abstract: OBJECTIVE
  To evaluate the effect of ischemic postconditioning(IPC) on intestinal mucosa of rats subjected to ischemia and reperfusion process comparing two cycles of reperfusion and ischemia lasting two minutes each and four cycles of reperfusion and ischemia lasting 30 seconds each.
  
  
  METHODS
  Thirty Wistar rats were distributed into three groups: group A (10 rats), ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion plus IPC by two lasting two minutes each; and Group C (10 rats), ischemia and reperfusion plus IPC by four cycles lasting 30 seconds each. Finally, a segment of small intestine was resected for histological analysis. We analysed the results according to Chiu et al. classification and proceeded to the statistical treatment by Kruskal-Wallis test (p<0.05).
  
  
  RESULTS
  The mean degree of tissue injury according to Chiu et al. classification were: Group A, 2.77; in group B, 1.4; and group C, 1.4. B X C (p<0.05).
  
  
  CONCLUSIONS
  Ischemic postconditioning was able to minimize reperfusion injury of rats undergone mesenteric ischemia and reperfusion process. There was no difference in the effectiveness of the method comparing two cycles of two minutes with four cycles of 30 seconds by H&E histological evaluation of the ileum after 60-minute reperfusion.
authors.affiliation: Department of Surgery, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Carlos Henrique Marques dos
authors.initials: CH
authors.lastname: Santos
authors.affiliation: Department of Surgery, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors.forename: Ricardo Dutra
authors.initials: RD
authors.lastname: Aydos
authors.affiliation: Department of Surgery, UFMS, Campo Grande, MS, Brazil.
authors.forename: Ed
authors.initials: E
authors.lastname: Nogueira Neto
authors.affiliation: Department of Pathology, UFMS, Campo Grande, MS, Brazil.
authors.forename: Luciana Nakao Odashiro
authors.initials: LN
authors.lastname: Miiji
authors.affiliation: UFMS, Campo Grande, MS, Brazil.
authors.forename: Pedro Carvalho
authors.initials: PC
authors.lastname: Cassino
authors.affiliation: School of Medicine, UFMS, Campo Grande, MS, Brazil.
authors.forename: Isadora Ishaq
authors.initials: II
authors.lastname: Ahmed
authors.affiliation: School of Medicine, UFMS, Campo Grande, MS, Brazil.
authors.forename: Nadia Meneguesso
authors.initials: NM
authors.lastname: Calheiros
authors.affiliation: School of Medicine, UFMS, Campo Grande, MS, Brazil.
authors.forename: Milena
authors.initials: M
authors.lastname: Garcia
authors.affiliation: UFMS, Campo Grande, MS, Brazil.
authors.forename: Anderson Fernandes da
authors.initials: AF
authors.lastname: Silva
mesh_terms: D000818:Animals; D007082:Ileum / Q000098:blood supply / Q000473:pathology; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D065666:Mesenteric Ischemia / Q000473:pathology / Q000517:prevention & control*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013997:Time Factors