title: Conditioned medium from human bone marrow-derived mesenchymal stem cells promotes skin moisturization and effacement of wrinkles in UVB-irradiated SKH-1 hairless mice.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) are promising therapeutic agents for various diseases.
  
  
  OBJECTIVE
  To investigate the effects of conditioned medium from human bone marrow-derived mesenchymal stem cells (MSC-CdM) on pro-collagen production and wrinkle formation, we performed in vitro and in vivo experiments.
  
  
  METHODS
  We assessed the effects of MSC-CdM on proliferation and photo-aging in human dermal fibroblasts after UVB exposure using enzyme activity assays for collagen type I secretion and MMP-1. To determine the effect of topically applied MSC-CdM on wrinkle formation, MSC-CdM (1% and 10%) and vehicle (propylene glycol: ethanol, 7 : 3) were applied to the dorsal skin of UVB-irradiated hairless mice for 8 weeks. We examined the effects on wrinkle formation by assessing visual skin grading, replica, tape stripping, transepidermal water loss (TEWL), and skin hydration measurement. We also examined histology of the lesions using hematoxylin-eosin, Masson's trichrome, and immunohistochemical staining.
  
  
  RESULTS
  MSC-CdM markedly reduced UV-induced matrix metalloproteinase-1 expression and increased pro-collagen synthesis in a dose-dependent manner. Our findings suggest that MSC-CdM induces repair of dermal damage and effacement of wrinkles on UVB-irradiated hairless mice through protective effect of hydration.
  
  
  CONCLUSIONS
  These results support an anti-wrinkle effect of MSC-CdM that involves increased collagen synthesis and suggest that MSC-CdM might be a potential candidate for preventing UV-induced skin damage.
authors.affiliation: Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea.|Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Tae-Rin
authors.identifier: http://orcid.org/0000-0002-9892-7714
authors.initials: TR
authors.lastname: Kwon
authors.affiliation: Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea.|Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Chang Taek
authors.initials: CT
authors.lastname: Oh
authors.affiliation: Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Eun Ja
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Soon Re
authors.initials: SR
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Jang
authors.affiliation: Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Ko
authors.affiliation: Department of Dermatology, College of Medicine, Catholic Kwandong University, International St. Mary's Hospital, Incheon, Korea.
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Yoo
authors.affiliation: Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea.|Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
authors.forename: Beom Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004547:Elastic Tissue / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D059630:Mesenchymal Stem Cells; D051379:Mice; D008812:Mice, Hairless; D011347:Procollagen / Q000096:biosynthesis; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D024042:Collagen Type I; D017077:Culture Media, Conditioned; D011347:Procollagen; D020781:Matrix Metalloproteinase 1
keywords: MSC-CdM; UVB radiation; antiwrinkle; photoaging