title: Functional and structural characterization of a heparanase.
abstract: We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Lisa
authors.initials: L
authors.lastname: Bohlmann
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Tredwell
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Xing
authors.identifier: http://orcid.org/0000-0002-6775-7062
authors.initials: X
authors.lastname: Yu
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chang
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Moritz
authors.initials: M
authors.lastname: Winger
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Dyason
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Thomson
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Joe
authors.initials: J
authors.lastname: Tiralongo
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Ifor R
authors.initials: IR
authors.lastname: Beacham
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Helen
authors.initials: H
authors.lastname: Blanchard
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland, Australia.
authors.forename: Mark
authors.identifier: http://orcid.org/0000-0001-6302-7524
authors.initials: M
authors.lastname: von Itzstein
mesh_terms: D016957:Burkholderia pseudomallei / Q000201:enzymology*; D018360:Crystallography, X-Ray; D005966:Glucuronidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D000099175:Heparanase