title: The diagnosis value of promoter methylation of UCHL1 in the serum for progression of gastric cancer.
abstract: BACKGROUND
  Aberrant promoter methylation has been considered as a potential molecular marker for gastric cancer (GC). However, the role of methylation of FLNC, THBS1, and UCHL1 in the development and progression of GC has not been explored.
  
  
  METHODS
  The promoter methylation status of UCHL1, FLNC, THBS1, and DLEC1 was assessed by quantitative methylation-specific PCR (QMSP) in the serum of 82 GC patients, 46 chronic atrophic gastritis (CAG) subjects, and 40 healthy controls.
  
  
  RESULTS
  All four genes had significantly higher methylation levels in GC patients than in CAG and control subjects. However, only UCHL1 methylation was significantly correlated with the tumor stage and lymph node metastasis. While THBS1 methylation was altered in an age-dependent manner, FLNC methylation was correlated with differentiation and Helicobacter pylori infection. DLEC1 methylation was only associated with tumor size. Moreover, methylated UCHL1 with or without THBS1 in the serum was found to be significantly associated with a poor prognosis.
  
  
  CONCLUSIONS
  The promoter methylation degree of FLNC, THBS1, UCHL1, and DLEC1 in serum could tell the existence of GC and only UCHL1 in the serum was also associated with poor prognosis of GC.
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Gongping
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Canhui
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Zengfang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Yantong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Oncology Surgery, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Feng
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002681:China / Q000453:epidemiology; D019175:DNA Methylation / Q000235:genetics; D018450:Disease Progression; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D015995:Prevalence; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology*; D043222:Ubiquitin Thiolesterase / Q000097:blood / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; C482860:UCHL1 protein, human; D043222:Ubiquitin Thiolesterase