title: Salmonella Levels in Turkey Neck Skins, Drumstick Bones, and Spleens in Relation to Ground Turkey.
abstract: The objective of this study was to determine Salmonella levels (presence and numbers) in turkey drumstick bone, spleen, and neck skin samples in relation to Salmonella contamination levels in ground turkey at the flock level. Over a 10-month period, a total of 300 samples of each turkey part (i.e., neck skin, spleen, and drumstick) from 20 flocks were collected at a commercial turkey processing plant after the evisceration step. Turkey flocks included in this study were classified as "targeted" and "nontargeted" based on the company's historical ground turkey contamination data. A flock that originated from a turkey farm that had previously produced one or more flocks with ≥20% Salmonella prevalence in ground turkey was labeled as a targeted flock (n = 13). The remaining seven flocks with <20% prevalence were labeled as nontargeted. All samples collected were tested for Salmonella presence and numbers by using most-probable-number and selective enrichment methods. Further genotypic analysis (pulsed-field gel electrophoresis) of the isolates was performed. Ground turkey samples were collected and analyzed for Salmonella levels by the cooperating turkey company. The outside surface of bone and spleen were sterilized prior to Salmonella analysis. The overall Salmonella prevalence in neck skin, drumstick bone, spleen, and ground turkey samples was 42.0, 9.3, 6.7, and 14.5%, respectively. Salmonella prevalence in neck skin, spleen, drumstick bone, and ground turkey from the targeted flocks was significantly (P < 0.05) higher than those from nontargeted flocks. There was a significant relationship between Salmonella presence in neck skin (when most probable numbers were ≥2 log) and Salmonella-positive ground turkey lot. Based on our findings, Salmonella was detected internally in drumstick bones and spleens at low levels, whereas Salmonella presence at higher levels in neck skin may indicate a flock with greater potential for Salmonella contamination of ground turkey.
authors.affiliation: Center for Food Safety, University of Georgia, Griffin, Georgia 30223, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Center for Food Safety, University of Georgia, Griffin, Georgia 30223, USA; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Dicle University Diyarbakir, 21280, Turkey.
authors.forename: Husnu S
authors.initials: HS
authors.lastname: Guran
authors.affiliation: Department of Food Science and Technology, University of Georgia, Athens, Georgia 30605, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Harrison
authors.affiliation: Poultry Diagnostic Research Center, Department of Population Health, University of Georgia, Athens, Georgia 30605, USA.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Hofacre
authors.affiliation: Center for Food Safety, University of Georgia, Griffin, Georgia 30223, USA. walali@uga.edu.
authors.forename: Walid Q
authors.initials: WQ
authors.lastname: Alali
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods*; D006801:Humans; D008460:Meat / Q000382:microbiology*; D009333:Neck / Q000382:microbiology; D015995:Prevalence; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012867:Skin / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology*; D014422:Turkeys / Q000382:microbiology