title: PCR-Based Simple Subgrouping Is Validated for Classification of Gliomas and Defines Negative Prognostic Copy Number Aberrations in IDH Mutant Gliomas.
abstract: Genetic subgrouping of gliomas has been emphasized recently, particularly after the finding of isocitrate dehydrogenase 1 (IDH1) mutations. In a previous study, we investigated whole-chromosome copy number aberrations (CNAs) of gliomas and have described genetic subgrouping based on CNAs and IDH1 mutations. Subsequently, we classified gliomas using simple polymerase chain reaction (PCR)-based methods to improve the availability of genetic subgrouping. We selected IDH1/2 and TP53 as markers and analyzed 237 adult supratentorial gliomas using Sanger sequencing. Using these markers, we classified gliomas into three subgroups that were strongly associated with patient prognoses. These included IDH mutant gliomas without TP53 mutations, IDH mutant gliomas with TP53 mutations, and IDH wild-type gliomas. IDH mutant gliomas without TP53 mutations, which mostly corresponded to gliomas carrying 1p19q co-deletions, showed lower recurrence rates than the other 2 groups. In the other high-recurrence groups, the median progression-free survival (PFS) and overall survival (OS) of patients with IDH mutant gliomas with TP53 mutations were significantly longer than those of patients with IDH wild-type gliomas. Notably, most IDH mutant gliomas with TP53 mutations had at least one of the CNAs +7q, +8q, -9p, and -11p. Moreover, IDH mutant gliomas with at least one of these CNAs had a significantly worse prognosis than did other IDH mutant gliomas. PCR-based mutation analyses of IDH and TP53 were sufficient for simple genetic diagnosis of glioma that were strongly associated with prognosis of patients and enabled us to detect negative CNAs in IDH mutant gliomas.
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Nakae
authors.affiliation: Department of Neurosurgery, Keio University, Tokyo, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Sasaki
authors.affiliation: Department of Neurosurgery, Keio University, Tokyo, Japan.
authors.forename: Saeko
authors.initials: S
authors.lastname: Hayashi
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Hattori
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kumon
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Nishiyama
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Adachi
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Nagahisa
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Joji
authors.initials: J
authors.lastname: Inamasu
authors.affiliation: Department of Pathology, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Abe
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: Department of Neurosurgery, Fujita Health University, Toyoake, Aichi, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hirose
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D002869:Chromosome Aberrations; D004252:DNA Mutational Analysis; D018572:Disease-Free Survival; D005091:Exons; D005260:Female; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009154:Mutation; D060787:Neoplasm Grading; D009364:Neoplasm Recurrence, Local; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C538784:IDH2 protein, human; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human