title: Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor.
abstract: The interface between the endoplasmic reticulum (ER) and mitochondria referred to as the MAM (mitochondria-associated ER membrane) plays important roles in many physiological functions. A specific marker for this important entity of cellular structure is urgently needed. Thus, we propose in this method chapter that the membrane-bound ER chaperone sigma-1 receptor serves as an ideal marker for the MAM. We describe in detail the preparation and purification of the MAM by using the sigma-1 receptor as the marker and demonstrate the uniqueness of this marker by using a variety of cells, peripheral and neuronal.
authors.affiliation: Cellular Pathobiology Section, IRP, DHHS, NIDA, NIH, 333 Cassell Drive, Baltimore, MD, 21224, USA.
authors.forename: Abasha
authors.initials: A
authors.lastname: Lewis
authors.affiliation: Cellular Pathobiology Section, IRP, DHHS, NIDA, NIH, 333 Cassell Drive, Baltimore, MD, 21224, USA.
authors.forename: Shang-Yi
authors.initials: SY
authors.lastname: Tsai
authors.affiliation: Cellular Pathobiology Section, IRP, DHHS, NIDA, NIH, 333 Cassell Drive, Baltimore, MD, 21224, USA. tsu@intra.nida.nih.gov.
authors.forename: Tsung-Ping
authors.initials: TP
authors.lastname: Su
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D017480:Receptors, sigma / Q000378:metabolism*; D000097605:Sigma-1 Receptor
chemical_list: D017480:Receptors, sigma; D000097605:Sigma-1 Receptor
keywords: Chaperone; Endoplasmic reticulum; Fractionation; MAM; Mitochondria; SDS/PAGE electrophoresis; Sigma-1 receptor