title: Gene-expression profile of developing pollen tube of Pyrus bretschneideri.
abstract: Pollen is an ideal model system for investigation of cell growth. In order to better understand the molecular biology mechanisms of the process of pear pollen tube development, RNA sequencing (RNA-Seq) technology was used to characterize the expression of genes during four development stages of pear pollen, including mature pollen grains (MP), hydrated pollen grains (HP), growing pollen tubes (PT) and stopped-growth pollen tubes (SPT). The four libraries generated a total of 47,072,151 clean reads that were mapped and assembled into 21,394 genes. Transcripts from the four stages were classified into 38 functional subcategories. Between MP and HP, 305 genes were differentially expressed, and 502 genes were differentially expressed between HP and PT. More importantly, we have observed that 2208 genes were differentially expressed between PT and SPT, and this is the first report of the gene expression comparison between the two development stages. Eight of the differentially expressed genes were randomly selected to confirm the RNA-Seq results by quantitative real-time PCR (qRT-PCR). Taken together, this research provides a platform for future research on pear pollen tube growth and growth cessation.
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: juyouwu@njau.edu.cn.
authors.forename: Juyou
authors.initials: J
authors.lastname: Wu
authors.affiliation: Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: slzhang@njau.edu.cn.
authors.forename: Shaoling
authors.initials: S
authors.lastname: Zhang
mesh_terms: D020869:Gene Expression Profiling; D017343:Genes, Plant; D063245:Plant Development; D053205:Pollen Tube / Q000235:genetics*; D031989:Pyrus / Q000235:genetics*; D018749:RNA, Plant; D017423:Sequence Analysis, RNA
chemical_list: D018749:RNA, Plant
keywords: Development; Gene expression profile; Pear; Pollen tube