title: A procoagulant chemically sulfated mannan.
abstract: Disorders of hemostasis can produce innumerous problems. Polysaccharides have been studied both as anticoagulant and as procoagulant agents. A mannan with a main chain of α-(1 → 6)-linked-Manp units, branched at O-2 mainly by side-chains of 2-O-linked-α-Manp units was chemically sulfated, structurally characterized by NMR and GC-MS (methylation, desulfation and methylation with trideuterated iodomethane), and tested in vitro and in vivo on blood coagulation models. Chemical analyses indicate a high degree of substitution on the sulfated polysaccharide. This polymer acted as a procoagulant agent, increasing blood coagulation in normal and hemophilic plasma, activated platelet aggregation and also decreased ex vivo aPTT. Polymers such as the sulfated mannan could be a helpful source of hemostatic agents to prevent hemorrhagic states.
authors.affiliation: Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: anagracher@gmail.com.
authors.forename: Ana Helena P
authors.initials: AH
authors.lastname: Gracher
authors.affiliation: Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: alinebiomed.ufpr@gmail.com.
authors.forename: Aline G
authors.initials: AG
authors.lastname: Santana
authors.affiliation: Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: trcipriani@ufpr.br.
authors.forename: Thales R
authors.initials: TR
authors.lastname: Cipriani
authors.affiliation: Laboratório de Química de Carboidratos, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná CEP 81531-980, Brazil. Electronic address: iacomini@ufpr.br.
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D005174:Factor XII / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D005260:Female; D006467:Hemophilia A / Q000503:physiopathology; D006801:Humans; D006847:Hydrocarbons, Iodinated / Q000737:chemistry; D008351:Mannans / Q000737:chemistry* / Q000494:pharmacology*; D008745:Methylation; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000737:chemistry*; D013917:Thrombin / Q000378:metabolism; D020246:Venous Thrombosis / Q000503:physiopathology
chemical_list: D006847:Hydrocarbons, Iodinated; D008351:Mannans; D013431:Sulfates; D005174:Factor XII; C014055:methyl iodide; D013917:Thrombin; D015951:Factor Xa
keywords: Chemical analysis; Chemically sulfated polysaccharide; Methylation; Procoagulant effect