title: Sustainable production of valuable compound 3-succinoyl-pyridine by genetically engineering Pseudomonas putida using the tobacco waste.
abstract: Treatment of solid and liquid tobacco wastes with high nicotine content remains a longstanding challenge. Here, we explored an environmentally friendly approach to replace tobacco waste disposal with resource recovery by genetically engineering Pseudomonas putida. The biosynthesis of 3-succinoyl-pyridine (SP), a precursor in the production of hypotensive agents, from the tobacco waste was developed using whole cells of the engineered Pseudomonas strain, S16dspm. Under optimal conditions in fed-batch biotransformation, the final concentrations of product SP reached 9.8 g/L and 8.9 g/L from aqueous nicotine solution and crude suspension of the tobacco waste, respectively. In addition, the crystal compound SP produced from aqueous nicotine of the tobacco waste in batch biotransformation was of high purity and its isolation yield on nicotine was 54.2%. This study shows a promising route for processing environmental wastes as raw materials in order to produce valuable compounds.
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Tang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D061249:Batch Cell Culture Techniques; D055162:Biocatalysis; D001673:Biodegradation, Environmental; D005818:Genetic Engineering; D007220:Industrial Waste; D009682:Magnetic Resonance Spectroscopy; D009538:Nicotine / Q000737:chemistry / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D016958:Pseudomonas putida / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D012037:Refuse Disposal; D021241:Spectrometry, Mass, Electrospray Ionization; D013386:Succinates / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014026:Nicotiana / Q000737:chemistry* / Q000378:metabolism
chemical_list: C507268:6-hydroxy-3-succinoyl-pyridine; D001426:Bacterial Proteins; D007220:Industrial Waste; D011725:Pyridines; D013386:Succinates; D009538:Nicotine; C023666:pyridine