title: Continuous desulfurization and bacterial community structure of an integrated bioreactor developed to treat SO2 from a gas stream.
abstract: Sulfide dioxide (SO2) is often released during the combustion processes of fossil fuels. An integrated bioreactor with two sections, namely, a suspended zone (SZ) and immobilized zone (IZ), was applied to treat SO2 for 6months. Sampling ports were set in both sections to investigate the performance and microbial characteristics of the integrated bioreactor. SO2 was effectively removed by the synergistic effect of the SZ and IZ, and more than 85% removal efficiency was achieved at steady state. The average elimination capacity of SO2 in the bioreactor was 2.80g/(m(3)·hr) for the SZ and 1.50g/(m(3)·hr) for the IZ. Most SO2 was eliminated in the SZ. The liquid level of the SZ and the water content ratio of the packing material in the IZ affected SO2 removal efficiency. The SZ served a key function not only in SO2 elimination, but also in moisture maintenance for the IZ. The desired water content in IZ could be feasibly maintained without any additional pre-humidification facilities. Clone libraries of 16S rDNA directly amplified from the DNA of each sample were constructed and sequenced to analyze the community composition and diversity in the individual zones. The desulfurization bacteria dominated both zones. Paenibacillus sp. was present in both zones, whereas Ralstonia sp. existed only in the SZ. The transfer of SO2 to the SZ involved dissolution in the nutrient solution and biodegradation by the sulfur-oxidizing bacteria. This work presents a potential biological treatment method for waste gases containing hydrophilic compounds.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: leel@rcees.ac.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Ding
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Junxin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D057927:Hydrophobic and Hydrophilic Interactions; D013458:Sulfur Dioxide / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D000393:Air Pollutants; D014867:Water; D013458:Sulfur Dioxide
keywords: Biological deodorization; Clone library; DNA sequence analysis; Integrated bioreactor; Sulfur bacteria; Sulfur dioxide