title: Evaluation of antimicrobial resistance of Helicobacter pylori in the last 15 years in West Poland.
abstract: Increasing resistance to drugs represents a serious problem in treatment of infections with Helicobacter pylori, providing cause of frequent therapeutic failures. Present study aimed at analysis of changes in resistance of H. pylori to antibiotics in West Poland within the recent 15 years. 108 strains of H. pylori were analysed, isolated from gastric mucosa of adult patients. Group 1 involved 66 strains isolated in years of 1998/1999. Group 2 comprised 42 isolates obtained in years of 2013/2014. Susceptibility to amoxicillin (AMX), clarithromycin (CL), tetracycline (TC) and metronidazole (MTZ) was determined by E-test (AB Biodisc). All strains on both studied groups were susceptible to AMX. In group 1 all strains proved to be susceptible to TC, while 9% and 36% of tested strains were resistant to CL and MTZ, respectively. By contrast, in group 2, 31% and 83% of strains were resistant to CL and MTZ, respectively. In parallel, 14% strains were found to be resistant to TC (according to EUCAST interpretations). In West Poland, within recent 15 years a dramatic increase was noted in H. pylori strains resistant to metronidazole. In parallel, a significant increase was noted in proportion of strains resistant to clarithromycin.
authors.affiliation: Department of Medical Microbiology, Poznań University of Medical Sciences , Wieniawskiego Str. 3, 61-712 Poznań , Poland.
authors.forename: Tomasz M
authors.initials: TM
authors.lastname: Karpiński
authors.affiliation: Department of Medical Microbiology, Poznań University of Medical Sciences , Wieniawskiego Str. 3, 61-712 Poznań , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Andrzejewska
authors.affiliation: Department of Gastroenterology, Human Nutrition and Internal Diseases, Poznań University of Medical Sciences , Przybyszewskiego Str. 49, 60-355 Poznań , Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Eder
authors.affiliation: Department of Gastroenterology, Human Nutrition and Internal Diseases, Poznań University of Medical Sciences , Przybyszewskiego Str. 49, 60-355 Poznań , Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Linke
authors.affiliation: Department of Medical Microbiology, Poznań University of Medical Sciences , Wieniawskiego Str. 3, 61-712 Poznań , Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szkaradkiewicz
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011044:Poland
chemical_list: D000900:Anti-Bacterial Agents; D008795:Metronidazole; D000658:Amoxicillin; D017291:Clarithromycin
keywords: Helicobacter pylori; antibiotic resistance; chronic gastritis; peptic ulcer disease; treatment