title: Paenibacillus cavernaesp. nov., isolated from soil of a natural cave.
abstract: A Gram-reaction-variable, strictly aerobic, motile, rod-shaped bacterium, designated strain C4-5T, was isolated from soil of a natural cave. Cells were oxidase- and catalase-positive and formed endospores in sporangia. The 16S rRNA gene sequence comparison showed that the organism formed a distinct clade within the genusPaenibacillusand was most closely related toPaenibacillus contaminansCKOBP-6T( = KCTC 13623T) (96.85 % 16S rRNA gene sequence similarity) followed byPaenibacillus doosanensisCAU 1005T( = KCTC 33036T) (94.82 %). The following chemotaxonomic features of strain C4-5Tare typical for the genusPaenibacillus:meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, the major fatty acids of anteiso-C15 : 0, iso-C16 : 0and C16 : 0and the DNA G+C content of 54.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminophospholipids. The phenotypic and phylogenetic data presented support that strain C4-5Trepresents a novel species of the genusPaenibacillus, for which the namePaenibacillus cavernaesp. nov. is proposed. The type strain is C4-5T( = KCTC 33652T= DSM 100100T).
authors.affiliation: Faculty of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Soon
authors.initials: S
authors.lastname: Dong Lee