title: Complete genome sequencing of Dehalococcoides sp. strain UCH007 using a differential reads picking method.
abstract: A novel Dehalococcoides sp. strain UCH007 was isolated from the groundwater polluted with chlorinated ethenes in Japan. This strain is capable of dechlorinating trichloroethene, cis-1,2-dichloroethene and vinyl chloride to ethene. Dehalococcoides bacteria are hardly cultivable, so genome sequencing has presented a challenge. In this study, we developed a differential reads picking method for mixed genomic DNA obtained from a co-culture, and applied it to the sequencing of strain UCH007. The genome of strain UCH007 consists of a 1,473,548-bp chromosome that encodes 1509 coding sequences including 29 putative reductive dehalogenase genes. Strain UCH007 is the first strain in the Victoria subgroup found to possess the pceA, tceA and vcrA genes.
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Uchino
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Miura
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosoyama
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Ohji
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamazoe
authors.affiliation: Taisei Corporation, 344-1 Nase, Kanagawa, 245-0051 Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Ito
authors.affiliation: Taisei Corporation, 344-1 Nase, Kanagawa, 245-0051 Japan.
authors.forename: Yoh
authors.initials: Y
authors.lastname: Takahata
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation, 2-10-49 Nishihara, Tokyo, 151-0066 Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Fujita
keywords: Bioremediation; Chloroethene; Dehalococcoides; Dehalorespiring; Differential reads picking method; Reductive dechlorination