title: 3D rotating wall vessel and 2D cell culture of four veterinary virus pathogens: A comparison of virus yields, portions of infectious particles and virus growth curves.
abstract: Only very few comparative studies have been performed that evaluate general trends of virus growth under 3D in comparison with 2D cell culture conditions. The aim of this study was to investigate differences when four animal viruses are cultured in 2D and 3D. Suid herpesvirus 1 (SuHV-1), Vesicular stomatitis virus (VSIV), Bovine adenovirus (BAdV) and Bovine parainfluenza 3 virus (BPIV-3) were cultivated in 3D rotating wall vessels (RWVs) and conventional 2D cultures. The production of virus particles, the portion of infectious particles, and the infectious growth curves were compared. For all viruses, the production of virus particles (related to cell density), including the non-infectious ones, was lower in 3D than in 2D culture. The production of only infectious particles was significantly lower in BAdV and BPIV-3 in 3D cultures in relation to cell density. The two cultivation approaches resulted in significantly different virus particle-to-TCID50 ratios in three of the four viruses: lower in SuHV-1 and BPIV-3 and higher in BAdV in 3D culture. The infectious virus growth rates were not significantly different in all viruses. Although 3D RWV culture resulted in lower production of virus particles compared to 2D systems, the portion of infectious particles was higher for some viruses.
authors.affiliation: Collection of Animal Pathogenic Microorganisms, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic. Electronic address: Malenovska@vri.cz.
authors.forename: Hana
authors.initials: H
authors.lastname: Malenovská
mesh_terms: D000818:Animals; D046808:Atadenovirus / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002522:Chlorocebus aethiops; D004285:Dogs; D011558:Herpesvirus 1, Suid / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D061985:Madin Darby Canine Kidney Cells; D029101:Parainfluenza Virus 3, Bovine / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D013552:Swine; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
keywords: 3D virus culture; Bovine adenovirus; Bovine parainfluenza 3 virus; Suid herpesvirus 1; Vesicular stomatitis virus