title: Alginate stabilized gold nanoparticle as multidrug carrier: Evaluation of cellular interactions and hemolytic potential.
abstract: This work delineates the synthesis of curcumin (Ccm) and methotrexate (MTX) conjugated biopolymer stabilized AuNPs (MP@Alg-Ccm AuNPs). The dual drug conjugated nano-vector was characterized by FTIR, (1)H NMR and UV-vis spectroscopic techniques. Hydrodynamic diameter and surface charge of the AuNPs were determined by DLS analysis and the spherical particles were visualized by TEM. MP@Alg-Ccm AuNPs exhibited improved cytotoxic potential against C6 glioma and MCF-7 cancer cell lines and was found to be highly hemocompatible. MP@Alg-Ccm AuNPs also exhibited active targeting efficiency against MCF-7 cancer cells due to the presence of "antifolate" drug MTX. Thus MP@Alg-Ccm AuNPs may find potential application in targeted combination chemotherapy for the treatment of cancer. The study is also interesting from the synthetic point of view because, here generation of AuNPs was done using "green chemical" alginate and dual drug conjugated AuNPs were created in two simple reaction steps using "green solvent" water.
authors.affiliation: Laboratory for Polymer Analysis, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum 695012, India.
authors.forename: Soma
authors.initials: S
authors.lastname: Dey
authors.affiliation: Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum 695012, Kerala, India.
authors.forename: M Caroline Diana
authors.initials: MC
authors.lastname: Sherly
authors.affiliation: Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Trivandrum 695012, Kerala, India.
authors.forename: M R
authors.initials: MR
authors.lastname: Rekha
authors.affiliation: Laboratory for Polymer Analysis, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Trivandrum 695012, India. Electronic address: sreeni@sctimst.ac.in.
authors.forename: K
authors.initials: K
authors.lastname: Sreenivasan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001692:Biological Transport; D003474:Curcumin / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity; D004355:Drug Stability; D020723:Glucuronic Acid / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity; D006461:Hemolysis / Q000187:drug effects*; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D008727:Methotrexate / Q000737:chemistry; D051381:Rats
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D006046:Gold; D020723:Glucuronic Acid; D003474:Curcumin; D008727:Methotrexate
keywords: Alginate; Confocal laser scanning microscopy; Curcumin; Gold nanoparticles; Methotrexate; Polymer–drug conjugates