title: Study of plasma-derived miRNAs mimic differences in Huntington's disease brain.
abstract: BACKGROUND
  Biomarkers for Huntington's disease progression could accelerate therapeutic developments and improve patient care. Brain microRNAs relating to clinical features of Huntington's disease may represent a potential Huntington's disease biomarker in blood.
  
  
  OBJECTIVE
  This study was undertaken to examine candidate microRNAs in plasma to determine whether changes observed in HD brains are detectable in peripheral samples.
  
  
  METHODS
  Four microRNAs from 26 manifest Huntington's disease, four asymptomatic Huntington's disease gene carriers, and eight controls were quantified in plasma using reverse transcription quantitative polymerase chain reaction. Linear regression was used to assess microRNA levels across control, asymptomatic gene carriers, and manifest patients.
  
  
  RESULTS
  miR-10b-5p (P = 0.0068) and miR-486-5p (P = 0.044) were elevated in Huntington's disease plasma. miR-10b-5p was decreased in asymptomatic gene carriers as compared with patients with Huntington's disease (P = 0.049), but no difference between asymptomatic gene carriers and healthy controls was observed (P = 0.24).
  
  
  CONCLUSIONS
  These findings suggest that microRNA changes observed in Huntington's disease brain may be detectable in plasma and have potential clinical utility.
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Hoss
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Valentina N
authors.initials: VN
authors.lastname: Lagomarsino
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Frank
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Tiffany C
authors.initials: TC
authors.lastname: Hadzi
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Richard H
authors.initials: RH
authors.lastname: Myers
authors.affiliation: Department of Neurology, Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Jeanne C
authors.initials: JC
authors.lastname: Latourelle
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D018450:Disease Progression; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Huntington's disease; biomarker; blood; miRNA; microRNA