title: Stimulation of the Antioxidative and Antimicrobial Potential of the Blood Red Bracket Mushroom Pycnoporus sanguineus (Higher Basidiomycetes).
abstract: The antioxidative and antibacterial properties of low-molecular-weight secondary metabolite subfractions (ex-LMS) from cultures of Pycnoporus sanguineus cultivated under different temperature conditions (25°C [ex-LMSa] and 30°C [ex-LMSb]) were assessed. The antioxidative properties were studied using chemiluminometric measurement, an ABTS assay, and a DPPH reduction rate assay with Trolox and ascorbic acid as the control. The values noted for the ex-LMSb were significantly higher than those for ex-LMSa: 97%, 52%, and 31% for chemiluminometric measurement, the ABTS assay, and the DPPH assay, respectively, at a concentration of 50 µg/mL. Half-maximal effective concentrations reached 4.17 µg/mL for chemiluminometric measurement, 47.25 µg/mL for the ABTS assay, and 51.46 µg/mL for DPPH assay. Toxicity tests against Vibrio fischeri yielded 99.8% for ex-LMSa and 99.85% for ex-LMSb. Antibacterial activity toward Staphylococcus aureus was observed in the ex-LMSb fractions (inhibition zone, 23.5 mm; minimum inhibitory concentration, 0.12 mg/mL). Scanning electron microscopy images exhibited severe disruption of the bacterial cells treated with ex-LMSb compared with the control. The results obtained suggest that the extracellular fluid isolated from P. sanguineus-submerged cultures might be a good source of antioxidative and antibacterial compounds. In addition, the increase in the culture temperature evidently enhanced the bioactive properties of the preparation.
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jaszek
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Osinska-Jaroszuk
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sulej
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Matuszewska
authors.affiliation: Department of Biochemistry, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Stefaniuk
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Maciag
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Polak
authors.affiliation: Pediatric Orthopedic and Rehabilitation Clinic, Medical University of Lublin, Lublin, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Matuszewski
authors.affiliation: Department of Biochemistry, Institute of Biology and Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, Lublin, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grzywnowicz
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D003470:Culture Media; D008826:Microbial Sensitivity Tests; D055494:Pycnoporus / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D003470:Culture Media