title: Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2 O2 ) accumulation in Brassica napus.
abstract: To achieve host colonization, successful pathogens need to overcome plant basal defences. For this, (hemi)biotrophic pathogens secrete effectors that interfere with a range of physiological processes of the host plant. AvrLm4-7 is one of the cloned effectors from the hemibiotrophic fungus Leptosphaeria maculans 'brassicaceae' infecting mainly oilseed rape (Brassica napus). Although its mode of action is still unknown, AvrLm4-7 is strongly involved in L. maculans virulence. Here, we investigated the effect of AvrLm4-7 on plant defence responses in a susceptible cultivar of B. napus. Using two isogenic L. maculans isolates differing in the presence of a functional AvrLm4-7 allele [absence ('a4a7') and presence ('A4A7') of the allele], the plant hormone concentrations, defence-related gene transcription and reactive oxygen species (ROS) accumulation were analysed in infected B. napus cotyledons. Various components of the plant immune system were affected. Infection with the 'A4A7' isolate caused suppression of salicylic acid- and ethylene-dependent signalling, the pathways regulating an effective defence against L. maculans infection. Furthermore, ROS accumulation was decreased in cotyledons infected with the 'A4A7' isolate. Treatment with an antioxidant agent, ascorbic acid, increased the aggressiveness of the 'a4a7' L. maculans isolate, but not that of the 'A4A7' isolate. Together, our results suggest that the increased aggressiveness of the 'A4A7' L. maculans isolate could be caused by defects in ROS-dependent defence and/or linked to suppressed SA and ET signalling. This is the first study to provide insights into the manipulation of B. napus defence responses by an effector of L. maculans.
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.|Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Prague, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Nováková
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Šašek
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Trdá
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.|Faculty of Science, Charles University in Prague, Prague, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Krutinová
authors.affiliation: INRA, UMR INRA-AgroParisTech 1290 Bioger, Avenue Lucien Brétignières, Thiverval-Grignon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Mongin
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Prague, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Valentová
authors.affiliation: INRA, UMR INRA-AgroParisTech 1290 Bioger, Avenue Lucien Brétignières, Thiverval-Grignon, France.
authors.forename: Marie-HelEne
authors.initials: MH
authors.lastname: Balesdent
authors.affiliation: INRA, UMR INRA-AgroParisTech 1290 Bioger, Avenue Lucien Brétignières, Thiverval-Grignon, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Rouxel
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Burketová
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000483:Alleles; D000975:Antioxidants / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001205:Ascorbic Acid / Q000494:pharmacology; D029688:Brassica napus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D002853:Chromatography, Liquid; D018548:Cotyledon / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D003517:Cyclopentanes / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D013058:Mass Spectrometry; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003517:Cyclopentanes; D005030:Ethylenes; D005656:Fungal Proteins; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D020156:Salicylic Acid; D001205:Ascorbic Acid
keywords: AvrLm4-7; Brassica napus; Leptosphaeria; ROS; effector; ethylene; salicylic acid