title: Using wastewater after lipid fermentation as substrate for bacterial cellulose production by Gluconacetobacter xylinus.
abstract: In this study, lipid fermentation wastewater (fermentation broth after separation with yeast biomass) with high Chemical Oxygen Demand (COD) value of 25,591 mg/L was used as substrate for bacterial cellulose (BC) production by Gluconacetobacter xylinus for the first time. After 5 days of fermentation, the highest BC yield (0.659 g/L) was obtained. Both monosaccharide and polysaccharides present in lipid fermentation wastewater could be utilized by G. xylinus simultaneously during fermentation. By this bioconversion, 30.0% of COD could be removed after 10 days of fermentation and the remaining wastewater could be used for further BC fermentation. The crystallinity of BC samples in lipid fermentation wastewater increased gradually during fermentation but overall the environment of lipid fermentation wastewater showed small influence on BC structure by comparison with that in traditional HS medium by using FE-SEM, FTIR, and XRD. By this work, the possibility of using lipid fermentation wastewater containing low value carbohydrate polymer (extracellular polysaccharides) for high value carbohydrate polymer (BC) production was proven.
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Guo
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Lian
authors.initials: L
authors.lastname: Xiong
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Si-Lan
authors.initials: SL
authors.lastname: Shi
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Xue-Fang
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Lin
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China; R&D Center of Xuyi Attapulgite Applied Technology, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Xuyi 211700, PR China. Electronic address: cxd_cxd@hotmail.com.
authors.forename: Xin-De
authors.initials: XD
authors.lastname: Chen
mesh_terms: D057919:Biological Oxygen Demand Analysis; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000378:metabolism*; D050356:Lipid Metabolism; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002482:Cellulose; D062065:Wastewater
keywords: Bacterial cellulose; Bio-polymer; Gluconacetobacter xylinus; Lipid fermentation wastewater