title: Peptide Functionalized Gold Nanorods for the Sensitive Detection of a Cardiac Biomarker Using Plasmonic Paper Devices.
abstract: The sensitivity of localized surface plasmon resonance (LSPR) of metal nanostructures to adsorbates lends itself to a powerful class of label-free biosensors. Optical properties of plasmonic nanostructures are dependent on the geometrical features and the local dielectric environment. The exponential decay of the sensitivity from the surface of the plasmonic nanotransducer calls for the careful consideration in its design with particular attention to the size of the recognition and analyte layers. In this study, we demonstrate that short peptides as biorecognition elements (BRE) compared to larger antibodies as target capture agents offer several advantages. Using a bioplasmonic paper device (BPD), we demonstrate the selective and sensitive detection of the cardiac biomarker troponin I (cTnI). The smaller sized peptide provides higher sensitivity and a lower detection limit using a BPD. Furthermore, the excellent shelf-life and thermal stability of peptide-based LSPR sensors, which precludes the need for special storage conditions, makes it ideal for use in resource-limited settings.
authors.affiliation: Institute of Material Science and Engineering and Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
authors.forename: Sirimuvva
authors.initials: S
authors.lastname: Tadepalli
authors.affiliation: Soft Matter Materials Branch, Materials and Manufacturing Directorate, Wright Patterson Air Force Base, Dayton, OH 45433, USA.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Kuang
authors.affiliation: Institute of Material Science and Engineering and Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Institute of Material Science and Engineering and Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
authors.forename: Keng-Ku
authors.initials: KK
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Division of Clinical and Translational Research, Washington University in St. Louis, St. Louis, MO 63110, USA.
authors.forename: Marilee A
authors.initials: MA
authors.lastname: Fisher
authors.affiliation: Department of Anesthesiology, Division of Clinical and Translational Research, Washington University in St. Louis, St. Louis, MO 63110, USA.|Siteman Cancer Center, Washington University in St. Louis, St. Louis, MO 63110, USA.
authors.forename: Jeremiah J
authors.initials: JJ
authors.lastname: Morrissey
authors.affiliation: Department of Anesthesiology, Division of Clinical and Translational Research, Washington University in St. Louis, St. Louis, MO 63110, USA.|Siteman Cancer Center, Washington University in St. Louis, St. Louis, MO 63110, USA.|Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO 63110, USA.
authors.forename: Evan D
authors.initials: ED
authors.lastname: Kharasch
authors.affiliation: Soft Matter Materials Branch, Materials and Manufacturing Directorate, Wright Patterson Air Force Base, Dayton, OH 45433, USA.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Slocik
authors.affiliation: Soft Matter Materials Branch, Materials and Manufacturing Directorate, Wright Patterson Air Force Base, Dayton, OH 45433, USA.
authors.forename: Rajesh R
authors.initials: RR
authors.lastname: Naik
authors.affiliation: Institute of Material Science and Engineering and Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.|Siteman Cancer Center, Washington University in St. Louis, St. Louis, MO 63110, USA.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Singamaneni
mesh_terms: D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D010209:Paper; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D020349:Surface Plasmon Resonance; D013542:Sweat / Q000378:metabolism; D019210:Troponin I / Q000097:blood
chemical_list: D015415:Biomarkers; D010455:Peptides; D019210:Troponin I; D006046:Gold