title: Analysis of T cell responses to chimpanzee adenovirus vectors encoding HIV gag-pol-nef antigen.
abstract: Adenoviruses have been shown to be both immunogenic and efficient at presenting HIV proteins but recent trials have suggested that they may play a role in increasing the risk of HIV acquisition. This risk may be associated with the presence of pre-existing immunity to the viral vectors. Chimpanzee adenoviruses (chAd) have low seroprevalence in human populations and so reduce this risk. ChAd3 and chAd63 were used to deliver an HIV gag, pol and nef transgene. ELISpot analysis of T cell responses in mice showed that both chAd vectors were able to induce an immune response to Gag and Pol peptides but that only the chAd3 vector induced responses to Nef peptides. Although the route of injection did not influence the magnitude of immune responses to either chAd vector, the dose of vector did. Taken together these results demonstrate that chimpanzee adenoviruses are suitable vector candidates for the delivery of HIV proteins and could be used for an HIV vaccine and furthermore the chAd3 vector produces a broader response to the HIV transgene.
authors.affiliation: School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, Surrey, UK.
authors.forename: S
authors.initials: S
authors.lastname: Herath
authors.affiliation: School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, Surrey, UK.
authors.forename: A
authors.initials: A
authors.lastname: Le Heron
authors.affiliation: ReiThera Srl, Viale Citta d'Europa 679, 00144 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Colloca
authors.affiliation: Department of Immunology, Imperial College London, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: Bergin
authors.affiliation: Department of Immunology, Imperial College London, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Patterson
authors.affiliation: Department of Immunology, Imperial College London, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Weber
authors.affiliation: Department of Immunology, Imperial College London, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Tatoud
authors.affiliation: School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, Surrey, UK. Electronic address: G.Dickson@rhul.ac.uk.
authors.forename: G
authors.initials: G
authors.lastname: Dickson
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000261:Adenoviruses, Simian / Q000235:genetics / Q000276:immunology*; D000818:Animals; D004337:Drug Carriers; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D005822:Genetic Vectors; D007371:Interferon-gamma / Q000378:metabolism; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D054301:gag Gene Products, Human Immunodeficiency Virus; D054311:nef Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus; D007371:Interferon-gamma
keywords: Adenovirus; HIV; Immunogenicity; Mouse; Vaccine; Vectors