title: New Class 2 Integron In2-4 Among IncI1-Positive Escherichia coli Isolates Carrying ESBL and PMAβ Genes from Food Animals in Portugal.
abstract: The impact of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (PMAβs) of animal origin constitutes a public health concern. In this study, 179 Escherichia coli from food animals and products were analyzed, among which, 15 cephalosporin-resistant isolates harboring ESBL (CTX-M-1 [n = 8], CTX-M-14 [n = 1], SHV-12 [n = 2]) or PMAβ [CMY-2, n = 5]) were identified in poultry and swine, from different farms of distinct regions of Portugal. The multiple sequence-type IncI1-driven spread of ESBLs and PMAβs, flanked by widely disseminated mobile elements, was guaranteed by ST26/IncI1-harboring blaSHV-12, ST12/IncI1-harboring blaCMY-2, ST3 and ST38/IncI1-harboring blaCTX-M-1, and ST1/IncI1-harboring blaCTX-M-14. An IS10-disrupted In2-4, presenting a new attI2 recombination site, was also detected in a SHV-12/CTX-M-1-harboring isolate. This study highlights the fact that animals may act as persistent sources of ESBL- and PMAβ-harboring plasmids genes that might be transferred to humans through direct contact or via the food chain.
authors.affiliation: 1 National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR/HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge , Lisbon, Portugal .|2 Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, Oporto University , Oporto, Portugal .
authors.forename: Daniela
authors.initials: D
authors.lastname: Jones-Dias
authors.affiliation: 1 National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR/HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge , Lisbon, Portugal .|2 Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, Oporto University , Oporto, Portugal .
authors.forename: Vera
authors.initials: V
authors.lastname: Manageiro
authors.affiliation: 3 Microbiology Laboratory , Controlvet, Tondela, Portugal .
authors.forename: Ana P
authors.initials: AP
authors.lastname: Martins
authors.affiliation: 1 National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR/HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge , Lisbon, Portugal .
authors.forename: Eugénia
authors.initials: E
authors.lastname: Ferreira
authors.affiliation: 1 National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR/HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge , Lisbon, Portugal .
authors.forename: Manuela
authors.initials: M
authors.lastname: Caniça
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011174:Portugal / Q000453:epidemiology; D011200:Poultry / Q000382:microbiology*; D013552:Swine; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; C055184:beta-lactamase TEM-3; C048795:AmpC beta-lactamases; D001618:beta-Lactamases