title: Proteome Differences in Placenta and Endometrium between Normal and Intrauterine Growth Restricted Pig Fetuses.
abstract: Uteroplacental tissue plays a key role in substance exchanges between maternal and fetal circulation, and, therefore, in the growth and development of fetuses. In this study, proteomics and western blotting were applied to investigate the changes of proteome in the placenta and endometrium of normal and intrauterine growth restriction (IUGR) porcine fetuses during mid to late pregnancy (D60, 90, and 110 of gestation). Our results showed that proteins participating in cell structure, energy metabolism, stress response, cell turnover, as well as transport and metabolism of nutrients were differentially expressed in placenta and endometrium between normal and IUGR fetuses. Analysis of functions of these proteins suggests reductions in ATP production and nutrients transport, increases in oxidative stress and apoptosis, and impairment of cell metabolism in IUGR fetuses. Collectively, our findings aid in understanding of the mechanisms responsible for uteroplacental dysfunction in IUGR fetus, and are expected to provide new strategies to reduce fetal growth restriction in pigs and other mammals.
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.|Department of Animal Science, South China Agricultural University, Guangzhou, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Taiji
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, China-Japan Friendship Hospital, Beijing, China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.|Department of Animal Science, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas, United States of America.
authors.forename: Gregory
authors.initials: G
authors.lastname: Johnson
authors.affiliation: State Key Laboratory of Animal Nutrition, Alltech-MAFIC Research Alliance, College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001724:Birth Weight; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine / Q000196:embryology*
chemical_list: D020543:Proteome