title: In vitro evaluation of anticancer and antibacterial activities of cobalt oxide nanoparticles.
abstract: Cobalt oxide nanoparticles (Co3O4-NPs) were synthesized using simple urea-based thermal decomposition method. Phase purity and particle size of as-synthesized nanoparticles were characterized through X-ray diffraction pattern (XRD) and transmission electron microscopy. Through XRD morphology of the Co3O4-NPs was found to be variable in size with range of 36 nm. In our present study, we explored the potential cytotoxic and antibacterial effects of Co3O4-NPs in human colorectal types of cancerous cells (HT29 and SW620) and also nine Gram-positive and Gram-negative bacteria. Co3O4-NPs showed promising anticancer activity against HT29 and SW620 cells with IC50 value of 2.26 and 394.5 μg/mL, respectively. However, no significant effect of Co3O4-NPs was observed against bacterial strains. Furthermore, a detailed study has been carried out to investigate the possible mechanism of cell death in HT29 cancer cell line through the analysis of expression level of anti-apoptotic Bcl2 and BclxL markers. Western blot analysis results suggested significant role of Co3O4-NPs exposure in cell death due to apoptosis.
authors.affiliation: Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia. khan.shahanavaj@gmail.com.|Department of Bioscience, Shri Ram Group of College (SRGC), Muzaffarnagar, UP, India. khan.shahanavaj@gmail.com.
authors.forename: Shahanavaj
authors.initials: S
authors.lastname: Khan
authors.affiliation: King Abdullah Institute for Nanotechnology, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
authors.forename: Anees A
authors.initials: AA
authors.lastname: Ansari
authors.affiliation: Nanomedicine and Biotechnology Research Unit, Department of Pharmaceutics, College of Pharmacy, King Saud University, PO Box 2457, Riyadh, 11451, Saudi Arabia.
authors.forename: Abdul Arif
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Colorectal Research Center, College of Medicine, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Rehan
authors.initials: R
authors.lastname: Ahmad
authors.affiliation: Colorectal Research Center, College of Medicine, King Saud University, Riyadh, 11451, Saudi Arabia.
authors.forename: Omar
authors.initials: O
authors.lastname: Al-Obaid
authors.affiliation: Department of Surgery, Al-Faisal University Riyadh, Riyadh, 11451, Saudi Arabia.
authors.forename: Wael
authors.initials: W
authors.lastname: Al-Kattan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001681:Biological Assay; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D010087:Oxides; D003035:Cobalt; C060728:cobalt oxide
keywords: Cobalt oxide nanoparticles; Cytotoxicity; MTT assay; Western blot analysis