title: [Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice].
abstract: OBJECTIVE
  To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.
  
  
  METHODS
  A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each): the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.
  
  
  RESULTS
  The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05).
  
  
  CONCLUSIONS
  The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.
authors.affiliation: Department of Parenteral and Enteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Qian-kun
authors.initials: QK
authors.lastname: Zhu
authors.affiliation: Department of Parenteral and Enteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Gui-zhen
authors.initials: GZ
authors.lastname: He
authors.affiliation: Department of Parenteral and Enteral Nutrition,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Li
mesh_terms: D000818:Animals; D018925:Chemokines; D004797:Enzyme-Linked Immunosorbent Assay; D036341:Intercellular Signaling Peptides and Proteins; D007422:Intestines / Q000293:injuries*; D007511:Ischemia; D051379:Mice; D008810:Mice, Inbred C57BL; D015427:Reperfusion Injury
chemical_list: D018925:Chemokines; D036341:Intercellular Signaling Peptides and Proteins; C523686:chemerin protein, mouse