title: Campylobacter species isolated from poultry and humans, and their analysis using PFGE in southern Brazil.
abstract: Campylobacteriosis is a bacterial disease transmitted to humans through ingestion of contaminated food. Six hundred samples were collected, 200 from human stool samples, 200 from poultry products and 200 from poultry feces in Southern Brazil, and then inoculated on blood agar plates. A total of 58% of the poultry feces, 17% of the poultry meat, and 2% the of human stools tested positive for Campylobacter. Positive Campylobacter colonies were identified as Campylobacter jejuni or Campylobactercoli by multiplex PCR. Campylobacter isolates were analyzed using PFGE to compare different profiles according to the source. This study demonstrated that there are different Campylobacter clones distributed in different aviaries in Southern Brazil. In addition, PFGE molecular profiles suggested that broilers can be a source of contamination of poultry products. However, the human isolate studied did not show any relationship with other strains examined.
authors.affiliation: Inspection of Animal Products, Universidade Federal de Pelotas, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS, Brazil. Electronic address: Daiani.teixeiradasilva@lshtm.ac.uk.
authors.forename: Daiani Teixeira da
authors.initials: DT
authors.lastname: Silva
authors.affiliation: Inspection of Animal Products, Universidade Federal de Pelotas, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: Talita Schneid
authors.initials: TS
authors.lastname: Tejada
authors.affiliation: Department of Microbiology and Parasitology, Universidade Federal de Pelotas, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: Dulcinea
authors.initials: D
authors.lastname: Blum-Menezes
authors.affiliation: Inspection of Animal Products, Universidade Federal de Pelotas, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: Priscila Alves
authors.initials: PA
authors.lastname: Dias
authors.affiliation: Inspection of Animal Products, Universidade Federal de Pelotas, Campus Universitário, S/N - CEP 96160-000, Capão do Leão, RS, Brazil.
authors.forename: Cláudio Dias
authors.initials: CD
authors.lastname: Timm
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001938:Brazil; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005260:Female; D005506:Food Contamination; D006801:Humans; D007223:Infant; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology; D011202:Poultry Products / Q000382:microbiology*; D055815:Young Adult
keywords: Campylobacter; Fecal; Humans; Meat; PFGE molecular profiles; Poultry