title: Burkholderia pseudomallei induces IL-23 production in primary human monocytes.
abstract: Burkholderia pseudomallei, a gram-negative intracellular bacterium, is a causative agent of melioidosis. The bacterium has been shown to induce the innate immune response, particularly pro-inflammatory cytokine production in several of both mouse and human cell types. In the present study, we investigate host immune response in B. pseudomallei-infected primary human monocytes. We discover that wild-type B. pseudomallei is able to survive and multiply inside the primary human monocytes. In contrast, B. pseudomallei LPS mutant, a less virulent strain, is susceptible to host killing during bacterial infection. Moreover, microarray result showed that wild-type B. pseudomallei but not B. pseudomallei LPS mutant is able to activate gene expression of IL-23 as demonstrated by the up-regulation of p19 and p40 subunit expression. Consistent with gene expression analysis, the secretion of IL-23 analyzed by ELISA also showed that wild-type B. pseudomallei induces a significantly higher level of IL-23 secretion than that of B. pseudomallei LPS mutant. These results implied that IL-23 may be an important cytokine for the innate immune response during B. pseudomallei infection. The regulation of IL-23 production may drive the different host innate immune responses between patients and may relate to the severity of melioidosis.
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Panthong
authors.initials: P
authors.lastname: Kulsantiwong
authors.affiliation: Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
authors.forename: Matsayapan
authors.initials: M
authors.lastname: Pudla
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Jitrada
authors.initials: J
authors.lastname: Boondit
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Chanthiwa
authors.initials: C
authors.lastname: Wikraiphat
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Susanna J
authors.initials: SJ
authors.lastname: Dunachie
authors.affiliation: Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.|Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.affiliation: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand. pongsak.uta@mahidol.ac.th.
authors.forename: Pongsak
authors.initials: P
authors.lastname: Utaisincharoen
mesh_terms: D016957:Burkholderia pseudomallei / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D006801:Humans; D007113:Immunity, Innate; D053760:Interleukin-23 Subunit p19 / Q000096:biosynthesis* / Q000378:metabolism; D046228:Microarray Analysis; D050296:Microbial Viability; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology*
chemical_list: C508840:IL23A protein, human; D053760:Interleukin-23 Subunit p19
keywords: B. pseudomallei; Human monocytes; IL-23; Melioidosis