title: Overexpression of Aiolos in Peripheral Blood Mononuclear Cell Subsets from Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis.
abstract: Genetic studies demonstrate that the Aiolos polymorphisms contribute to the susceptibility to autoimmune diseases. The purpose of the study was to investigate the Aiolos expression in lymphocytes and monocytes in the peripheral blood from patients with SLE and RA, and to explore the correlation between Aiolos expression in cell subsets and laboratory measurements. Peripheral blood mononuclear cells (PBMC) from 32 patients with SLE, 35 patients with RA, and 37 healthy controls were purified. Aiolos expression in PBMC subsets was examined by flow cytometry. In SLE patients, a much higher percentage of Aiolos + CD8+ T cells and Aiolos + CD14+ monocytes was found, when compared with healthy controls (p = 8.29 × 10(-5) and p = 1.01 × 10(-5), respectively). Furthermore, the percentage of CD4+ and CD8+ T cells, CD19+ B cells, and CD14+ monocytes expressing Aiolos in RA patients was also determined and each found higher than that in healthy controls (p = 0.009, p = 4.11 × 10(-5), p = 0.001, and p = 1.11 × 10(-5), respectively). The percentage of Aiolos + CD8+ T cells was weakly correlated with ESR in SLE patients and RF in RA patients (r s = 0.37, p = 0.038; r s = 0.34, p = 0.044, respectively). On the other hand, the percentage of Aiolos + CD14+ monocytes was significantly correlated with multiple laboratory measurements, including ESR, creatinine, CRP, LDH, proteinuria, albumin, and ACCPA in patients (r s = 0.62, p < 0.001; r s = 0.65, p < 0.001; r s = 0.44, p = 0.010; r s = 0.42, p = 0.022; r s = 0.52, p = 0.013; r s = 0.34, p = 0.048, respectively). To our knowledge, it is the first study to demonstrate overexpression of Aiolos in PBMC subsets in SLE and RA patients. The results indicate that overexpression of Aiolos may contribute to pathogenesis of SLE and RA.
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Xinze
authors.initials: X
authors.lastname: Cai
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Du
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Xu
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Ang
authors.initials: A
authors.lastname: Liu
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ge
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Central Laboratory, First Affiliated Hospital of China Medical University, Shenyang, 110001, China. xzcai@mail.cmu.edu.cn.|Department of Dermatology, First Affiliated Hospital of China Medical University, Shenyang, 110001, China. xzcai@mail.cmu.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D051740:Ikaros Transcription Factor / Q000097:blood* / Q000235:genetics; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D016131:Lymphocyte Subsets; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide
chemical_list: C106285:IKZF3 protein, human; D051740:Ikaros Transcription Factor
keywords: Aiolos; Mononuclear cell; Rheumatoid arthritis; Systemic lupus erythematosus