title: Identification of Ligustrum lucidum pollen allergens using a proteomics approach.
abstract: BACKGROUND
  Ligustrum spp. are members of the Oleaceae family, one of the most prominent allergic families worldwide. The genus Ligustrum contains approximately fifty species, including Ligustrum lucidum, which have been widely cultivated as ornamental plants, and its pollen is a source of inhalant allergens associated with respiratory allergic diseases. Little is known about the presence of allergenic proteins in L. lucidum.
  
  
  METHODS
  The L. lucidum pollen proteins were extracted by a modified phenolic extraction method. A pool of four sera from mono sensitive patients was analyzed by 2DE immunoblotting and mass spectrometric analysis was performed on 6 immunoreactive protein spots.
  
  
  RESULTS
  SDS-PAGE of L. lucidum pollen extract revealed proteins in ranges of 15-150 kDa. The 2DE gel profile of the L. lucidum pollen protein extract showed approximately 180 spots, and the 2DE immunoblots obtained using sera from Ligustrum monosensitive patients as the source of IgE antibodies revealed six allergen protein spots, corresponding to Profilin, Enolase, Fra e 9.01 (β-1,3-glucanase), Pollen-specific Polygalacturonases, Alanine aminotransferase, and two ATP synthase beta subunits.
  
  
  CONCLUSIONS
  We report for the first time the identification of IgE-reactive proteins from L. lucidum.
authors.affiliation: Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, C.P.11340 México City, Mexico.
authors.forename: Blessy Maruthukunnel
authors.initials: BM
authors.lastname: Mani
authors.affiliation: Centro de investigación en Alimentación y Desarrollo, A.C. Apartado Postal 1735, Hermosillo, Sonora, Mexico.
authors.forename: Jose Angel
authors.initials: JA
authors.lastname: Huerta-Ocampo
authors.affiliation: Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, C.P.11340 México City, Mexico.
authors.forename: Jose Ruben
authors.initials: JR
authors.lastname: Garcia-Sanchez
authors.affiliation: División Biología Molecular, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Col. Lomas 4a. Sección, 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Alberto
authors.initials: A
authors.lastname: Barrera-Pacheco
authors.affiliation: División Biología Molecular, IPICyT, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, Col. Lomas 4a. Sección, 78216 San Luis Potosí, SLP, Mexico.
authors.forename: Ana Paulina Barba
authors.initials: AP
authors.lastname: de la Rosa
authors.affiliation: Instituto Nacional de Enfermedades Respiratorias "Ismael Cosío Villegas", Calzada Tlalpan No. 4502, Sección XVI, C.P.14080 México City, Mexico. Electronic address: teranjlm@gmail.com.
authors.forename: Luis M
authors.initials: LM
authors.lastname: Teran
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D031659:Ligustrum / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D010940:Plant Proteins / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D000485:Allergens; D010940:Plant Proteins; D020543:Proteome
keywords: Immunoblotting; Ligustrum lucidum; Mass spectrometry; Pollen allergy; Two-dimensional gel electrophoresis