title: Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves.
abstract: Arsenic (As) contamination of water is a global concern and rice consumption is the biggest dietary exposure to human posing carcinogenic risks, predominantly in Asia. Sulfur (S) is involved in di-sulfide linkage in many proteins and plays crucial role in As detoxification. Present study explores role of variable S supply on rice leaf proteome, its inclination towards amino acids (AA) profile and non protein thiols under arsenite exposure. Analysis of 282 detected proteins on 2-DE gel revealed 113 differentially expressed proteins, out of which 80 were identified by MALDI-TOF-TOF. The identified proteins were mostly involved in glycolysis, TCA cycle, AA biosynthesis, photosynthesis, protein metabolism, stress and energy metabolism. Among these, glycolytic enzymes play a major role in AA biosynthesis that leads to change in AAs profiling. Proteins of glycolytic pathway, photosynthesis and energy metabolism were also validated by western blot analysis. Conclusively S supplementation reduced the As accumulation in shoot positively skewed thiol metabolism and glycolysis towards AA accumulation under AsIII stress.
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Garima
authors.initials: G
authors.lastname: Dixit
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Amit Pal
authors.initials: AP
authors.lastname: Singh
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Dwivedi
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Farah
authors.initials: F
authors.lastname: Deeba
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Kumar
authors.affiliation: CSIR-Indian Institute of Toxicology Research, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Shankar
authors.initials: S
authors.lastname: Suman
authors.affiliation: Rice Research Station, Government of West Bengal, Chinsurah, Hooghly - 712102, West Bengal, India.
authors.forename: Bijan
authors.initials: B
authors.lastname: Adhikari
authors.affiliation: CSIR-Indian Institute of Toxicology Research, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Yogeshwar
authors.initials: Y
authors.lastname: Shukla
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Pandey
authors.affiliation: CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, Uttar Pradesh, India.
authors.forename: Rudra Deo
authors.initials: RD
authors.lastname: Tripathi
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D018053:Arsenites / Q000633:toxicity*; D002952:Citric Acid Cycle / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D012275:Oryza / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013455:Sulfur / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000596:Amino Acids; D018053:Arsenites; D010940:Plant Proteins; D020543:Proteome; D013438:Sulfhydryl Compounds; D013455:Sulfur; D005978:Glutathione; C015001:arsenite