title: Nanofilms of hyaluronan/chitosan assembled layer-by-layer: An antibacterial surface for Xylella fastidiosa.
abstract: In this work, nanofilms of hyaluronan/chitosan (HA/CHI) assembled layer by layer were synthesized; their application as a potential antimicrobial material was demonstrated for the phytopathogen Xylella fastidiosa, a gram-negative bacterium, here used as a model. For the synthesis, the influence of pH and ionic strength of these natural polymer stem-solutions on final characteristics of the HA/CHI nanofilms was studied in detail. The antibacterial effect was evaluated using widefield fluorescence microscopy. These results were correlated with the chemical properties of the nanofilms, studied by FTIR and Raman spectroscopy, as well as with their morphology and surface properties characterized using SEM and AFM. The present findings can be extended to design and optimize HA/CHI nanofilms with enhanced antimicrobial behavior for other type of phytopathogenic gram-negative bacteria species, such as Xanthomonas citri, Xanthomas campestri and Ralstonia solanacearum.
authors.affiliation: Departamento de Física Aplicada, Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas, São Paulo, Brazil. Electronic address: jacobo@ifi.unicamp.br.
authors.forename: Jacobo
authors.initials: J
authors.lastname: Hernández-Montelongo
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, 13083-852 Campinas, São Paulo, Brazil.
authors.forename: Vicente F
authors.initials: VF
authors.lastname: Nascimento
authors.affiliation: Departamento de Física Aplicada, Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas, São Paulo, Brazil.
authors.forename: Duber
authors.initials: D
authors.lastname: Murillo
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, 13083-852 Campinas, São Paulo, Brazil.
authors.forename: Thiago B
authors.initials: TB
authors.lastname: Taketa
authors.affiliation: Departamento de Física Aplicada, Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas, São Paulo, Brazil.
authors.forename: Prasana
authors.initials: P
authors.lastname: Sahoo
authors.affiliation: Instituto Agronômico de Campinas, Centro de Apta Citros Sylvio Moreira, 13490-970 Cordeirópolis, São Paulo, Brazil.
authors.forename: Alessandra A
authors.initials: AA
authors.lastname: de Souza
authors.affiliation: Faculdade de Engenharia Química, Universidade Estadual de Campinas, 13083-852 Campinas, São Paulo, Brazil.
authors.forename: Marisa M
authors.initials: MM
authors.lastname: Beppu
authors.affiliation: Departamento de Física Aplicada, Instituto de Física Gleb Wataghin, Universidade Estadual de Campinas, 13083-859 Campinas, São Paulo, Brazil.
authors.forename: Monica A
authors.initials: MA
authors.lastname: Cotta
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D013499:Surface Properties; D044167:Xylella / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D006820:Hyaluronic Acid; D048271:Chitosan
keywords: Biopolymers; Hyaluronan/chitosan nanofilms; Layer-by-layer; Xylella fastidiosa