title: Synthesis and characterization of an in situ forming hydrogel using tyramine conjugated high methoxyl gum tragacanth.
abstract: In this study, an enzyme catalyzed in situ forming hydrogel based on tyramine conjugated high methoxyl content gum tragacanth (TA-HMGT) was prepared and characterized. TA-HMGT was synthesized via heterogeneous ammonolysis of methyl ester groups of HMGT. Then, the hydrogel was prepared via horseradish peroxidase catalyzed coupling reaction in the presence of hydrogen peroxide. Hydrogel properties, such as gelation time, swelling/degradation behavior and rheological properties could be adjusted by tuning the gelation parameters and extent of tyramine conjugation. This system was a soft elastic hydrogel with appropriate biocompatibility. The fast gelation of the hydrogel is desirable for clinical applications. Also, in vitro bovine serum albumin release from the synthesized hydrogel showed good release profile with limited burst release.
authors.affiliation: Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Moslem
authors.initials: M
authors.lastname: Tavakol
authors.affiliation: Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran evf@modares.ac.ir.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Vasheghani-Farahani
authors.affiliation: Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Mohammadifar
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Department of Nanotechnology, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
authors.forename: Sameereh
authors.initials: S
authors.lastname: Hashemi-Najafabadi
mesh_terms: D000818:Animals; D027883:Astragalus Plant / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D018938:Caco-2 Cells; D002417:Cattle; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D016503:Drug Delivery Systems; D004548:Elasticity; D004952:Esters / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology; D012212:Rheology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014144:Tragacanth / Q000737:chemistry*; D014439:Tyramine / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D004952:Esters; D020100:Hydrogels; D012710:Serum Albumin, Bovine; D014144:Tragacanth; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D014439:Tyramine
keywords: In situ forming hydrogel; ammonolysis; enzymatic gelation; high methoxyl tragacanth; tyramine