title: Mn(II) tags for DEER distance measurements in proteins via C-S attachment.
abstract: Mn(2+) chelating tags for Mn(2+)-Mn(2+) distance measurements by pulse EPR spectroscopy were developed. They feature a stable C-S conjugation to the protein, high reactivity towards cysteine thiols and short and rigid linkers that can be used in distance measurements with high resolution under reductive conditions. Double electron-electron resonance measurements at 95 GHz on ubiquitin labeled with these tags showed the expected narrow distance distribution.
authors.affiliation: Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel. daniella.goldfarb@weizmann.ac.il.
authors.forename: Andrea
authors.initials: A
authors.lastname: Martorana
authors.forename: Yin
authors.initials: Y
authors.lastname: Yang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Li
authors.forename: Xun-Cheng
authors.initials: XC
authors.lastname: Su
authors.forename: Daniella
authors.initials: D
authors.lastname: Goldfarb
mesh_terms: D002244:Carbon / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D008345:Manganese / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D025801:Ubiquitin; D008345:Manganese; D013455:Sulfur; D002244:Carbon; D003545:Cysteine