title: Designing idiosyncratic hmPCL-siRNA nanoformulated capsules for silencing and cancer therapy.
abstract: In this work, we have designed a siRNA-nanoformulation with mesoporous polycaprolactone (hmPCL) for silencing and cancer therapy. Average hollow core size of hmPCL nanocapsules used for nanoformulation is ~180 nm with shell thickness of 10-20 nm and mesopore size of ~5-10nm in diameter. Idiosyncratic capsules are biocompatible which has been confirmed with normal lymphocyte, K562 leukaemia cancer cells and on HepG2/EGFP cancer cells. In 1mg of hmPCL capsules up to 400 ng of siRNA can be loaded. This nanoformulation enables to tune the dose dependent delivery up to ~93.25% (373 ng) siRNA during therapy. hmPCL-siRNA nanoformulation mediated siRNA transfection on HepG2 cancer cells has been investigated and exhibited 32% silencing activity within 24h of post transfection. Obtained results directed us that the hmPCL-siRNA nanoformulation could be an efficient tool in siRNA mediated therapy for knocking down the infected cells.
  
  
  BACKGROUND
  siRNA could be used in cancer therapy if naked nucleic acid could be transported using a suitable carrier. In this article, the authors developed a nano-carrier system using mesoporous polycaprolactone (hmPCL) and showed its efficacy in knocking down cancer cells. This approach may open another way of gene therapy.
authors.affiliation: School of Engineering Sciences and Technology, University of Hyderabad, Hyderabad, India. Electronic address: vijaybhushan86@gmail.com.
authors.forename: Vijay Bhooshon
authors.initials: VB
authors.lastname: Kumar
authors.affiliation: School of Engineering Sciences and Technology, University of Hyderabad, Hyderabad, India. Electronic address: himadrim391@gmail.com.
authors.forename: Himadri
authors.initials: H
authors.lastname: Medhi
authors.affiliation: Cellular and Molecular Bioengineering, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore. Electronic address: biezy@nus.edu.sg.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yong
authors.affiliation: School of Engineering Sciences and Technology, University of Hyderabad, Hyderabad, India. Electronic address: ppse@uohyd.ernet.in.
authors.forename: Pradip
authors.initials: P
authors.lastname: Paik
mesh_terms: D045744:Cell Line, Tumor; D056945:Hep G2 Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry*; D009369:Neoplasms / Q000235:genetics / Q000628:therapy*; D011091:Polyesters / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics / Q000627:therapeutic use; D000067492:RNAi Therapeutics / Q000379:methods*; D014162:Transfection / Q000379:methods
chemical_list: D053769:Nanocapsules; D011091:Polyesters; D034741:RNA, Small Interfering; C016240:polycaprolactone
keywords: Cancer therapy; Hollow-mesoporous-PCL; Nanocapsules; Nanoformulation; siRNA-silencing