title: PFDN1, an indicator for colorectal cancer prognosis, enhances tumor cell proliferation and motility through cytoskeletal reorganization.
abstract: Prefoldin (PFDN) subunits have been reported upregulated in various tumor types, while the expression and functions of PFDN1 (PFDN subunit 1) in colorectal cancer (CRC) are not well elucidated. The aim of this study was to investigate the use of PFDN1 as a poor prognosis indicator for CRC and explore the functions of PFDN1 in CRC. The relationship between PFDN1 expression and CRC clinical-pathological statistics was detected on the tissue microarray containing 145 cases of CRC. ShRNA was used to silence PFDN1 expression in SW480 and RKO CRC cells, and these transfected cells were analyzed for changes in proliferation, colony formation, cell cycle, migration, and invasion. Immunofluorescence and immunoblot were used to determine the remodeling of the F-actin and α-tubulin. Finally, tumor growth on nude mice was observed and measured. In this study, we found PFDN1 was upregulated in CRC tissues compared with adjacent normal tissues. Also, PFDN1 expression positively correlated with tumor size and tumor invasion. Moreover, after silencing PFDN1 in SW480 and RKO cells, the proliferation and motility of CRC cells were significantly suppressed. The inhibitory effect of PFDN1 on tumor cell growth and motility was partially due to G2/M cell cycle blockage and cytoskeletal deficiency. Finally, in vivo assay showed that downregulation of PFDN1 inhibited tumor growth on nude mice and PFDN1 expression correlated with higher levels of Ki-67 staining. These findings indicate that PFDN1 was involved in the progression of CRC, and provide new insights into PFDN1 as a potential therapeutic target for CRC treatment.
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. wangpuxz@163.com.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China. wangpuxz@163.com.
authors.forename: Puxiongzhi
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Jingkun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Shaopei
authors.initials: S
authors.lastname: Guan
authors.affiliation: School of Medicine, University of Munich, Munich, Germany.
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai, China.
authors.forename: Dingpei
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Baochi
authors.initials: B
authors.lastname: Ou
authors.affiliation: Department of Thoracic Surgery, Ruijin Hospital, Shanghai, China.
authors.forename: Runsen
authors.initials: R
authors.lastname: Jin
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zong
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. marsnew1997@163.com.
authors.forename: Junjun
authors.identifier: http://orcid.org/0000-0002-7136-9345
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. adams_lu66@yahoo.com.cn.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China. adams_lu66@yahoo.com.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Lu
authors.affiliation: Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.|Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai, China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Zheng
mesh_terms: D000368:Aged; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality; D003599:Cytoskeleton / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D020868:Gene Silencing; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis
chemical_list: D018832:Molecular Chaperones; C112806:prefoldin
keywords: Colorectal cancer; Cytoskeletal reorganization; Migration and invasion; PFDN1; Proliferation