title: Capsular typing of Streptococcus pneumoniae isolated in an Algerian hospital using a new multiplex PCR-based scheme.
abstract: We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.
authors.affiliation: Service de Microbiologie Clinique, Centre Hospitalo-Universitaire Mustapha Bacha, Faculté de Médecine, Algiers, Algeria.
authors.forename: Hanifa
authors.initials: H
authors.lastname: Ziane
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal; CECA-ICETA, Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, Oporto University, Oporto, Portugal. Electronic address: vera.manageiro@insa.min-saude.pt.
authors.forename: Vera
authors.initials: V
authors.lastname: Manageiro
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Eugénia
authors.initials: E
authors.lastname: Ferreira
authors.affiliation: Service de Microbiologie Clinique, Centre Hospitalo-Universitaire Mustapha Bacha, Faculté de Médecine, Algiers, Algeria.
authors.forename: Soumia
authors.initials: S
authors.lastname: Bektache
authors.affiliation: Service de Microbiologie Clinique, Centre Hospitalo-Universitaire Mustapha Bacha, Faculté de Médecine, Algiers, Algeria.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Tazir
authors.affiliation: National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Caniça
mesh_terms: D000462:Algeria; D016667:Bacterial Capsules / Q000235:genetics* / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D006761:Hospitals; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D011008:Pneumococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
keywords: Multiplex PCR; Pneumococci; Quellung reaction; Sensitivity; Serogroup/type; Specificity