title: [Gene Mutations Associated with Macrolide-resistance and p1 Gene Typing of Mycoplasma pneumoniae Isolated in Yamagata, Japan, between 2004 and 2013].
abstract: To clarify the epidemiologic features of Mycoplasma pneumoniae, we examined 358 M. pneumoniae strains isolated between 2004 and 2013 in Yamagata, Japan. Analysis of macrolide-resistance-associated 23S ribosomal RNA (rRNA) domain V mutations revealed 6 kinds of mutants (81 A2063G, 43 A2063T, 1 A2063C, 1 A2064C, 4 C2617G and 1 C2617 mutation). There were only two mutants before 2009, but mutants A2063T and A2063G increased in 2009 and from 2010, respectively. The annual ratio of mutants varied from 20.4% to 76.4% between 2009 and 2013. Typing of the p1 gene revealed 4 types; 278 type 1, and 3 kinds of type 2 variant strains (10 type 2a, 5 type 2b and 65 type 2c). Type 1 strains accounted for between 85.2% and 100% of isolates from 2004 to 2011, whereas type 2 variant strains increased by 26.5% and 66.1% in 2012 and 2013, respectively. These results indicate that type 1 strains may have been replaced by type 2 variant strains in 2013. Furthermore, the ratio of type 1 strains with a 23S rRNA mutation was 65.1% in 2012 and 95.2% in 2013, but none of the type 2 variant strains had this mutation. In conclusion, type 1 strains with macrolide-resistant mutations appeared in 2006 and increased from 2009. In contrast, type 2 variant strains, which increased in 2012 and became predominant in 2013, showed no mutations.
authors.forename: Yu
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Junji
authors.initials: J
authors.lastname: Seto
authors.forename: Tutomu
authors.initials: T
authors.lastname: Itagaki
authors.forename: Toshiya
authors.initials: T
authors.lastname: Aoki
authors.forename: Chieko
authors.initials: C
authors.lastname: Abiko
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuzaki
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007564:Japan; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009154:Mutation / Q000235:genetics*; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D018942:Macrolides; D012338:RNA, Ribosomal, 23S