title: The Directional Observation of Highly Dynamic Membrane Tubule Formation Induced by Engulfed Liposomes.
abstract: Highly dynamic tubular structures in cells are responsible for exchanges between organelles. Compared with bacterial invasion, the most affordable and least toxic lipids were found in this study to be gentle and safe exogenous stimuli for the triggering of membrane tubules. A specific lipid system was internalized by NIH3T3 cells. Following cellular uptake, the constructed liposomes traveled towards the nucleus in aggregations and were gradually distributed into moving vesicles and tubules in the cytosol. The triggered tubules proceeded, retreated or fluctuated along the cytoskeleton and were highly dynamic, moving quickly (up to several microns per second), and breaking and fusing frequently. These elongated tubules could also fuse with one another, giving rise to polygonal membrane networks. These lipid systems, with the novel property of accelerating intracellular transport, provide a new paradigm for investigating cellular dynamics.
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.|National Lab for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.
authors.forename: Luru
authors.initials: L
authors.lastname: Dai
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.
authors.forename: Anhe
authors.initials: A
authors.lastname: Wang
authors.affiliation: Institute of Pharmacy, Martin Luther University, 06120 Halle (Saale), Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Wölk
authors.affiliation: Institute of Pharmacy, Martin Luther University, 06120 Halle (Saale), Germany.
authors.forename: Bodo
authors.initials: B
authors.lastname: Dobner
authors.affiliation: Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany.
authors.forename: Gerald
authors.initials: G
authors.lastname: Brezesinski
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.|Institute of Theoretical Physics, Chinese Academy of Sciences, 100190 Beijing, China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Tang
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.|Institute of Theoretical Physics, Chinese Academy of Sciences, 100190 Beijing, China.
authors.forename: Xianyou
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Center for Nanoscience and Technology, 100190 Beijing, China.|National Lab for Molecular Sciences, CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, 100190 Beijing, China.
authors.forename: Junbai
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001692:Biological Transport; D050356:Lipid Metabolism; D008081:Liposomes / Q000737:chemistry; D008566:Membranes / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D041681:NIH 3T3 Cells; D010455:Peptides / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D008081:Liposomes; D010455:Peptides