title: ZnO nanoparticles assist the refolding of denatured green fluorescent protein.
abstract: Proteins are essential for cellular and biological processes. Proteins are synthesized and fold into the native structure to become active. The inability of a protein molecule to remain in its native conformation is called as protein misfolding, and this is due to several environmental factors. Protein misfolding and aggregation handle several human diseases. Protein misfolding is believed to be one of the causes of several disorders such as cancer, degenerative diseases, and metabolic pathologies. The zinc oxide (ZnO) nanoparticle was significantly promoted refolding of thermally denatured green fluorescent protein (GFP). In the present study, ZnO nanoparticles interaction with GFP was investigated by ultraviolet-visible spectrophotometer, fluorescence spectrophotometer, and dynamic light scattering. Results suggest that the ZnO nanoparticles significantly assist the refolding of denatured GFP.
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, Korea.
authors.forename: Muthuraman
authors.initials: M
authors.lastname: Pandurangan
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, Korea.
authors.forename: Ahmad Jawid
authors.initials: AJ
authors.lastname: Zamany
authors.affiliation: Department of Bioresources and Food Science, Konkuk University, Seoul, Korea.
authors.forename: Doo Hwan
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000067493:Dynamic Light Scattering; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D011487:Protein Conformation; D058849:Protein Refolding / Q000187:drug effects*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D049452:Green Fluorescent Proteins; D015034:Zinc Oxide
keywords: DLS; GFP; ZnO nanoparticles; aggregation; misfolding