title: Glucose and fatty acid metabolism in infarcted heart from streptozotocin-induced diabetic rats after 2 weeks of tissue remodeling.
abstract: BACKGROUND
  The effects of streptozotocin (STZ)-induced diabetes on heart metabolism and function after myocardial infarction (MI) remodelling were investigated in rats.
  
  
  METHODS
  Fifteen days after STZ (50 mg/kg b.w. i.v.) injection, MI was induced by surgical occlusion of the left coronary artery. Two weeks after MI induction, contents of glycogen, ATP, free fatty acids and triacylglycerols (TG) and enzyme activities of glycolysis and Krebs cycle (hexokinase, glucose-6-phosphate dehydrogenase, phosphofructokinase, citrate synthase) and expression of carnitine palmitoyl-CoA transferase I (a key enzyme of mitochondrial fatty acid oxidation) were measured in the left ventricle (LV). Plasma glucose, free fatty acids and triacylglycerol levels were determined. Ejection fraction (EF) and shortening fraction (SF) were also measured by echocardiography.
  
  
  RESULTS
  Glycogen and TG contents were increased (p < 0.05) whereas ATP content was decreased in the LV of the non-infarcted diabetic group when compared to the control group (p < 0.05). When compared to infarcted control rats (MI), the diabetic infarcted rats (DI) showed (p < 0.05): increased plasma glucose and TG levels, elevated free fatty acid levels and increased activity of, citrate synthase and decreased ATP levels in the LV. Infarct size was smaller in the DI group when compared to MI rats (p < 0.05), and this was associated with higher EF and SF (p < 0.05).
  
  
  CONCLUSIONS
  Systolic function was preserved or recovered more efficiently in the heart from diabetic rats two weeks after MI, possibly due to the high provision of glucose and free fatty acids from both plasma and heart glycogen and triacylglycerol stores.
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. chrismalfi@hotmail.com.|Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. chrismalfi@hotmail.com.
authors.forename: Christiane
authors.initials: C
authors.lastname: Malfitano
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. lescanoalcione9@gmail.com.|Nursing Department, State University of Mato Grosso, Alta Floresta, Brazil. lescanoalcione9@gmail.com.
authors.forename: Alcione Lescano
authors.initials: AL
authors.lastname: de Souza Junior
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. mari.carbonaro@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Carbonaro
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. andressalopes1@yahoo.com.br.
authors.forename: Andressa
authors.initials: A
authors.lastname: Bolsoni-Lopes
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. diego.figueroa07@gmail.com.
authors.forename: Diego
authors.initials: D
authors.lastname: Figueroa
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. expleandro@hotmail.com.
authors.forename: Leandro Ezequiel
authors.initials: LE
authors.lastname: de Souza
authors.affiliation: Department of Nephrology, School of Medicine of the Federal, University of Sao Paulo, Sao Paulo, Brazil. kleitonedf@gmail.com.
authors.forename: Kleiton Augusto Santos
authors.initials: KA
authors.lastname: Silva
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. fernanda.consolim@incor.usp.br.|Laboratory of Translational Physiology, Universidade Nove de Julho, (UNINOVE), Sao Paulo, Brazil. fernanda.consolim@incor.usp.br.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Consolim-Colombo
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. ruicuri@icb.usp.br.
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
authors.affiliation: Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, 05403-000, Sao Paulo, SP, Brazil. hipirigoyen@gmail.com.|Laboratory of Translational Physiology, Universidade Nove de Julho, (UNINOVE), Sao Paulo, Brazil. hipirigoyen@gmail.com.
authors.forename: Maria Claudia
authors.initials: MC
authors.lastname: Irigoyen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002952:Citric Acid Cycle; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000150:complications; D004734:Energy Metabolism; D005227:Fatty Acids / Q000097:blood*; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis; D008297:Male; D009203:Myocardial Infarction / Q000097:blood* / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013318:Stroke Volume; D013599:Systole; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; D017382:Reactive Oxygen Species; D014280:Triglycerides; D000255:Adenosine Triphosphate; D006003:Glycogen; D002334:Carnitine O-Palmitoyltransferase