title: A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor.
abstract: OBJECTIVE
  To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source.
  
  
  RESULTS
  Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml(-1) within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium. A novel isoenzyme, named Tvlac, was identified, purified and characterized. Its optimum pH and temperature were from 4.5 to 5 and 55 to 60 °C, respectively. Its activity was stimulated by ethanol at 10 % (v/v) which increased the V 0.
  
  
  CONCLUSIONS
  The biochemical properties of Tvlac substantiate the potential of this enzyme for applications under an aqueous ethanol mixture environment.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yi
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Fajun
authors.initials: F
authors.lastname: Deng
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry & Biochemistry, Florida International University, Miami, FL, 33199, USA.
authors.forename: Xiaotang
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Fang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.|Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D010367:Arachis / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D020536:Enzyme Activators / Q000378:metabolism*; D004795:Enzyme Stability; D000431:Ethanol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D055454:Trametes / Q000201:enzymology* / Q000235:genetics; D014908:Triticum / Q000378:metabolism
chemical_list: D003470:Culture Media; D020536:Enzyme Activators; D000431:Ethanol; D042845:Laccase
keywords: Ethanol tolerance; Isoenzymes of laccase; Laccase; Trametes versicolor