title: Analysis of key genes of jasmonic acid mediated signal pathway for defense against insect damages by comparative transcriptome sequencing.
abstract: Corn defense systems against insect herbivory involve activation of genes that lead to metabolic reconfigurations to produce toxic compounds, proteinase inhibitors, oxidative enzymes, and behavior-modifying volatiles. Similar responses occur when the plant is exposed to methyl jasmonate (MeJA). To compare the defense responses between stalk borer feeding and exogenous MeJA on a transcriptional level, we employed deep transcriptome sequencing methods following Ostrinia furnacalis leaf feeding and MeJA leaf treatment. 39,636 genes were found to be differentially expressed with O. furnacalis feeding, MeJA application, and O. furnacalis feeding and MeJA application. Following Gene Ontology enrichment analysis of the up- or down- regulated genes, many were implicated in metabolic processes, stimuli-responsive catalytic activity, and transfer activity. Fifteen genes that indicated significant changes in the O. furnacalis feeding group: LOX1, ASN1, eIF3, DXS, AOS, TIM, LOX5, BBTI2, BBTI11, BBTI12, BBTI13, Cl-1B, TPS10, DOX, and A20/AN1 were found to almost all be involved in jasmonate defense signaling pathways. All of the data demonstrate that the jasmonate defense signal pathway is a major defense signaling pathways of Asian corn borer's defense against insect herbivory. The transcriptome data are publically available at NCBI SRA: SRS965087.
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, Heilongjiang 150080, China.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Qixing
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.|Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yin
authors.affiliation: Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA.
authors.forename: Kayla K
authors.initials: KK
authors.lastname: Pennerman
authors.affiliation: Department of Agriculture, ARS, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, USA.
authors.forename: Jiujiang
authors.initials: J
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin, Heilongjiang 150080, China.
authors.forename: Dafei
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
authors.forename: Anping
authors.initials: A
authors.lastname: Guo
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D003517:Cyclopentanes / Q000494:pharmacology*; D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D059014:High-Throughput Nucleotide Sequencing; D006790:Host-Parasite Interactions; D007313:Insecta; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000187:drug effects*; D059467:Transcriptome; D003313:Zea mays / Q000235:genetics* / Q000469:parasitology
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid