title: Metabolic Remodeling of Cell-Surface Sialic Acids: Principles, Applications, and Recent Advances.
abstract: Cell-surface sialic acids are essential in mediating a variety of physiological and pathological processes. Sialic acid chemistry and biology remain challenging to investigate, demanding new tools for probing sialylation in living systems. The metabolic glycan labeling (MGL) strategy has emerged as an invaluable chemical biology tool that enables metabolic installation of useful functionalities into cell-surface sialoglycans by "hijacking" the sialic acid biosynthetic pathway. Here we review the principles of MGL and its applications in study and manipulation of sialic acid function, with an emphasis on recent advances.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Xie
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Dong
authors.affiliation: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. xingchen@pku.edu.cn.
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
mesh_terms: D002236:Carbohydrate Conformation; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D011134:Polysaccharides; D012794:Sialic Acids
keywords: bioorthogonal chemistry; chemical reporters; click chemistry; metabolic glycan labeling; sialic acids