title: In vivo regeneration of renal vessels post whole decellularized kidneys transplantation.
abstract: Nearly 50 million patients in China live with end-stage renal disease (ESRD), and only about 4000 patients may receive kidney transplantation. The purpose of this study was to investigate regeneration of renal vessels post whole decellularized kidneys transplantation in vivo. We decellularized kidneys of donor rats by perfusing a detergent through the abdominal aorta, yielding feasible extracellular matrix, confirmed for acellularity before transplantation. Based on the concept of using the body as a bioreactor, we orthotopically transplanted the kidney and ureter scaffolds in recipient rats, and found the regeneration of vessels including artery and vein in the renal sinus following a spontaneous recanalization. Although the findings only represent an initial step toward the ultimate goal of the generation of fully functional kidneys in vivo, these findings suggest that the body itself, as the bioreactor, is a viable strategy for kidney regeneration.
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: JianSe
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.
authors.forename: ZhiBin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.
authors.forename: KeZhi
authors.initials: K
authors.lastname: Lin
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: YaLing
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: LiNa
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
authors.forename: TingGang
authors.initials: T
authors.lastname: Chu
authors.affiliation: Department of Hand and Foot Surgery, Luqiao Hospital of Enze Medical Center, Taizhou, China.
authors.forename: LiZhi
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Department of Anatomy, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
authors.forename: Ali
authors.initials: A
authors.lastname: Alkhawaji
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.
authors.forename: MiaoZhong
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.
authors.forename: YingKuan
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: XinFa
authors.initials: X
authors.lastname: Lou
authors.affiliation: Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: ShiXin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: MaoLin
authors.initials: M
authors.lastname: Tang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.|Institute of Neuroscience, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Mei
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology* / Q000502:physiology; D016030:Kidney Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D054457:Tissue Scaffolds
keywords: Pathology Section; microsurgery; orthotopic transplantation; renal regeneration; renal vessels; scaffold