title: 2-(Nitroaryl)benzothiazole and benzoxazole derivatives as fluorogenic substrates for the detection of nitroreductase activity in clinically important microorganisms.
abstract: A series of carboxy-substituted 2-(nitroaryl)benzothiazole derivatives and carboxy-substituted 2-(nitroaryl)benzoxazole derivatives were prepared and evaluated as potential nitroreductase substrates for the purpose of detecting clinically important microorganisms. Several of the substrates produced highly fluorescent colonies with the majority of a panel of 10 Gram-negative bacteria and also with two of a panel of 8 Gram-positive bacteria.
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Cellier
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK; École Nationale Supérieure de Chimie de Paris, 11 rue Pierre et Marie Curie, F75231 Paris Cedex 05, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Gignoux
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Arthur L
authors.initials: AL
authors.lastname: James
authors.affiliation: Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Orenga
authors.affiliation: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Perry
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Shaun N
authors.initials: SN
authors.lastname: Robinson
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK. Electronic address: steven.stanforth@northumbria.ac.uk.
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Stanforth
authors.affiliation: Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK.
authors.forename: Graeme
authors.initials: G
authors.lastname: Turnbull
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D052160:Benzothiazoles / Q000737:chemistry* / Q000378:metabolism; D001583:Benzoxazoles / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000201:enzymology; D006094:Gram-Positive Bacteria / Q000201:enzymology; D009601:Nitroreductases / Q000032:analysis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D052160:Benzothiazoles; D001583:Benzoxazoles; D005456:Fluorescent Dyes; D009601:Nitroreductases; C005465:benzothiazole
keywords: Bacterial detection; Enzyme substrates; Fluorescence; Microorganism detection; Nitroreductase