title: VEGF silencing inhibits human osteosarcoma angiogenesis and promotes cell apoptosis via PI3K/AKT signaling pathway.
abstract: Vascular endothelial growth factor (VEGF) is one of the most potently angiogenic factors which promotes generation of tumor vasculature. VEGF is usually up-regulated in multiple cancers include osteosarcoma and gliomas. To further explore the potential molecular mechanism that inhibits tumor growth induced by interference of VEGF expression, we constructed an Lv-shVEGF vector and assessed the efficiency of VEGF silencing and its influence on U2OS cells. Our data demonstrated that Lv-shVEGF has high inhibition efficiency on VEGF expression, which inhibits proliferation and promotes apoptosis of U2OS cells in vitro. Our results also indicated that inhibition of VEGF expression suppresses osteosarcoma tumor growth in vivo, VEGF inhibition reduces osteosarcoma angiogenesis. We also found that the phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) activation was considerably reduced after osteosarcoma cells were treated with Lv-shVEGF. Taken together, our data demonstrated that VEGF silencing suppresses cells proliferation, promotes cells apoptosis and reduces osteosarcoma angiogenesis through inactivation of PI3K/AKT signaling pathway.
authors.affiliation: Department of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University Xi'an 710038, Shaanxi Province, P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University Xi'an 710038, Shaanxi Province, P. R. China.
authors.forename: Zi-Ru
authors.initials: ZR
authors.lastname: Zhang
authors.affiliation: Department of Outpatient, Tangdu Hospital, Fourth Military Medical University Xi'an 710038, Shaanxi Province, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University Xi'an 710038, Shaanxi Province, P. R. China.
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Ma
authors.affiliation: Department of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, Tangdu Hospital, Fourth Military Medical University Xi'an 710038, Shaanxi Province, P. R. China.
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Fan
keywords: Cell apoptosis; U2OS cells; angiogenesis; lentivirus-mediated short hairpin RNA (Lv-shRNA); vascular endothelial growth factor (VEGF)