title: Osteogenesis of peripheral blood mesenchymal stem cells in self assembling peptide nanofiber for healing critical size calvarial bony defect.
abstract: Peripheral blood mesenchymal stem cells (PBMSCs) may be easily harvested from patients, permitting autologous grafts for bone tissue engineering in the future. However, the PBMSC's capabilities of survival, osteogenesis and production of new bone matrix in the defect area are still unclear. Herein, PBMSCs were seeded into a nanofiber scaffold of self-assembling peptide (SAP) and cultured in osteogenic medium. The results indicated SAP can serve as a promising scaffold for PBMSCs survival and osteogenic differentiation in 3D conditions. Furthermore, the SAP seeded with the induced PBMSCs was splinted by two membranes of poly(lactic)-glycolic acid (PLGA) to fabricate a composited scaffold which was then used to repair a critical-size calvarial bone defect model in rat. Twelve weeks later the defect healing and mineralization were assessed by H&E staining and microcomputerized tomography (micro-CT). The osteogenesis and new bone formation of grafted cells in the scaffold were evaluated by immunohistochemistry. To our knowledge this is the first report with solid evidence demonstrating PBMSCs can survive in the bone defect area and directly contribute to new bone formation. Moreover, the present data also indicated the tissue engineering with PBMSCs/SAP/PLGA scaffold can serve as a novel prospective strategy for healing large size cranial defects.
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.|Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Pan
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xianghai
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Jinkun
authors.initials: J
authors.lastname: Wen
authors.affiliation: Guangdong Institutes of Biomedicine and health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Shangtao
authors.initials: S
authors.lastname: Cao
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Changhui
authors.initials: C
authors.lastname: Qian
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.|State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.|GHM Institute of CNS Regeneration, Jinan University, Guangzhou 510632, China.
authors.forename: Wutian
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.|Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou 510515, China.|Institute of Bone Biology, Academy of Orthopaedics, Guangdong Province, Guangzhou 510665, China.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001773:Blood Cells / Q000166:cytology; D001861:Bone Regeneration / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002478:Cells, Cultured; D019344:Lactic Acid / Q000737:chemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011446:Prospective Studies; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology / Q000502:physiology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D015703:Antigens, CD; D010455:Peptides; C546681:RADA16-I; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid