title: Claudin 8 Contributes to Malignant Proliferation in Human Osteosarcoma U2OS Cells.
abstract: Human osteosarcoma (OS) represents one of the most common primary sarcomas often originating in the metaphyses of long bones. However, its underlying molecular pathogenesis is still only vaguely understood. Several tight junction proteins were shown to be associated with and involved in tumorigenesis. This study is aimed to evaluate the role of Claudin 8 (CLDN8) in human OS. Lentivirus-based short hairpin RNA targeting CLDN8 specifically depleted its endogenous expression in U2OS and SW1353 OS cells, with a reduction by 97.7% and 89.3%, respectively, in contrast to control. Depletion of CLDN8 led to a significant diminution in cell viability and proliferation. To test the mechanism by which CLDN8 modulates cell proliferation, the flow cytometry assay and apoptosis assay were performed and confirmed that G1-S transition was blocked and a strong proapoptotic effect was induced in U2OS cells by CLDN8 knockdown. These data demonstrate that CLDN8 plays an essential role in OS proliferation in vitro, which will provide a new opportunity for discovering and identifying novel effective treatment strategies.
authors.affiliation: 1 Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, China .
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Xu
authors.affiliation: 1 Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, China .
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: 1 Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, China .
authors.forename: Ping
authors.initials: P
authors.lastname: Hao
authors.affiliation: 2 Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, China .
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Ding
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D049109:Cell Proliferation; D057167:Claudins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D057167:Claudins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C502011:claudin 8
keywords: CLDN8; cell cycle; osteosarcoma; proliferation