title: Effects of milk components and food additives on survival of three bifidobacteria strains in fermented milk under simulated gastrointestinal tract conditions.
abstract: BACKGROUND
  In the dairy industry, probiotic strains of Bifidobacterium are introduced into the composition of traditional starter cultures intended for the production of fermented foods, or sometimes are the sole microflora responsible for the fermentation process. In order to be able to reach the intestines alive and fulfil their beneficial role, probiotic strains must be able to withstand the acidity of the gastric juices and bile present in the duodenum.
  
  
  OBJECTIVE
  The paper reports effects of selected fermented milk components on the viability of three strains of bifidobacteria in fermented milk during subsequent incubation under conditions representing model digestive juices.
  
  
  METHODS
  The viability of the bifidobacterial cells was examined after a 3-h incubation of fermented milk under simulated gastric juice conditions and then after 5-h incubation under simulated duodenum juice conditions. The Bifidobacterium strains tested differed in their sensitivity to the simulated conditions of the gastrointestinal juices.
  
  
  RESULTS
  Bifidobacterial cell viability in simulated intestinal juices was dependent on the strain used in our experiments, and product components acted protectively towards bifidobacterial cells and its dose.
  
  
  CONCLUSIONS
  Bifidobacterial cells introduced into the human gastrointestinal tract as food ingredients have a good chance of survival during intestinal transit and to reach the large intestine thanks to the protective properties of the food components and depending on the strain and composition of the food.
authors.affiliation: Division of Milk Biotechnology, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie, Warsaw, Poland; malgorzata_ziarno@sggw.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Ziarno
authors.affiliation: Division of Milk Biotechnology, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie, Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Zaręba
keywords: Bifidobacterium; fruit jam; gastrointestinal conditions; milk protein; prebiotic; whey protein