title: Impacts of phosphatase and tensin homology deleted on chromosome ten (PTEN)-inhibiting chitosan scaffold on growth and differentiation of neural stem cells.
abstract: OBJECTIVE
  The aim of this study was to investigate growth and differentiation of neural stem cells (NSCs) on the phosphatase and tensin homology deleted on chromosome ten (PTEN)-inhibitor-adsorbed chitosan scaffold.
  
  
  METHODS
  NSCs were divide into the chitosan group and the control groups, and performed CCK-8 test on 1(st), 3(rd) and 7(th) d to compare the proliferation between the 2 groups. The chitosan scaffold adsorbed PTEN inhibitor bpv (pic), and the empty scaffold was used as the control for co-culture of NSCs, immunofluorescence staining was performed on 7(th) d to detect the differentiation of NSCs on the scaffold.
  
  
  RESULTS
  The results of CCK-8 test showed no significant difference in the absorbance between the 2 groups. Immunofluorescence staining showed that the NSCs numbers of the bpv scaffold group were more than the empty scaffold group, among which the anti-glial fibrillary acidic protein (GFAP) positive cells were less than the empty scaffold group, while the anti-β-Tubulin III positive cells were more than the empty scaffold group, the two groups both showed rare anti-receptor-interacting protein (RIP) positive cells.
  
  
  CONCLUSIONS
  Chitosan scaffold exhibited good compatibility to NSCs, the PTEN-inhibitor-adsorbed chitosan scaffold could promote the migration of NSCs towards the scaffold and their differentiation towards neurons.
authors.affiliation: Department of Neurosurgery, Affiliated Haian Hospital of Nantong University Nantong 226001, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Fu
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yao
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Yili Xinhua Hospital Yining 835000, China.
authors.forename: Gequan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University Nantong 226001, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shen
keywords: PTEN; chitosan; neural stem cells; neural tissue engineering; tumor suppressor gene