title: [Adaptation of Anoxybacillus flavithermus ssp. yunnanesis E13(T) to toluene at the level of fatty acid composition of membrane].
abstract: OBJECTIVE
  Anoxybacillus flavithermus subsp. yunnanensis is now the only species of thermophilic bacteria able to tolerate toxic solvents at high temperature. The adaptive responses of A. flavithermus subsp. yunnanensis E13(T) to toluene on the level of fatty acid composition of membrane were studied in detail.
  
  
  METHODS
  The extraction of fatty acids was performed according to the method described in the Sherlock Microbial Identification System manual. The fatty acid compositions were analyzed by gas chromatography mass spectrometry (GC-MS).
  
  
  RESULTS
  In presence of 0.3% (V/V) toluene, key moment to adapt the saturated straight-chain fatty acids was that when cells grew from the lag phase to the initial growth phase in liquid. The saturated straight-chain fatty acids were continuously decreased as the strain E13(T) to grow. In survival of the cells in 100% toluene, the saturated straight-chain fatty acids increased significantly.
  
  
  CONCLUSIONS
  A. flavithermus ssp. yunnanesis E13(T) alters its membrane fluidity via fatty acid composition to become more rigid when it is exposed to solvent, which is consistent that commonly found in mesophilic organic solvent-tolerant bacteria. However, it adapted its membrane by increasing straight-chain saturated fatty acids, rather than unsaturated fatty acids, which was demonstrated in mesophilic organic solvent-tolerant bacteria.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Zheng
authors.forename: Maojiao
authors.initials: M
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
mesh_terms: D000222:Adaptation, Physiological; D058677:Anoxybacillus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008560:Membrane Fluidity; D014050:Toluene / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D014050:Toluene