title: Transamniotic stem cell therapy (TRASCET) mitigates bowel damage in a model of gastroschisis.
abstract: OBJECTIVE
  We sought to determine whether intraamniotic delivery of concentrated amniotic-derived mesenchymal stem cells (afMSCs) could reduce damage to exposed bowel in experimental gastroschisis.
  
  
  METHODS
  Rat fetuses (n=117) with surgically created gastroschisis were divided into three groups: untreated animals (n=62) and two groups receiving volume-matched intraamniotic injections of either saline (n=25) or 2 × 10(6) cells/mL of syngeneic, labeled afMSCs (n=30). Animals were killed before term, along with normal controls (NL). Blinded observers performed computerized measurements of total and segmental (serosa, muscularis, and mucosa) intestinal wall thicknesses. Statistical comparisons were by ANOVA (P<0.05).
  
  
  RESULTS
  Among survivors with gastroschisis, there were statistically significant decreases in total bowel wall, serosal, muscular, and mucosal thicknesses in the afMSC group vs. the untreated group (P=0.001/0.035/0.001/0.005, respectively) and vs. the saline group (P=0.003/0.05/<0.001/0.026, respectively). There were no such significant differences between the untreated and saline groups. There were no differences between the afMSC group and NL, except for a significantly thicker muscular layer in the afMSC group (P=0.014). Labeled afMSCs were scarcely identified, suggesting a paracrine effect.
  
  
  CONCLUSIONS
  Amniotic mesenchymal stem cells mitigate bowel damage in experimental gastroschisis after concentrated intraamniotic injection. Transamniotic stem cell therapy (TRASCET) may become a practical component of the treatment of gastroschisis.
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: Christina
authors.initials: C
authors.lastname: Feng
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Graham
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: John Patrick
authors.initials: JP
authors.lastname: Connors
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Brazzo
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: Amy H S
authors.initials: AH
authors.lastname: Pan
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: James R
authors.initials: JR
authors.lastname: Hamilton
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA.
authors.forename: David
authors.initials: D
authors.lastname: Zurakowski
authors.affiliation: Department of Surgery, Boston Children's Hospital and Harvard Medical School, Boston, MA. Electronic address: dario.fauza@childrens.harvard.edu.
authors.forename: Dario O
authors.initials: DO
authors.lastname: Fauza
mesh_terms: D000650:Amnion; D000653:Amniotic Fluid / Q000166:cytology; D000818:Animals; D005260:Female; D046128:Fetal Therapies / Q000379:methods*; D020139:Gastroschisis / Q000473:pathology / Q000628:therapy*; D007267:Injections; D007422:Intestines / Q000473:pathology*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016896:Treatment Outcome
keywords: Amniotic mesenchymal stem cells; Fetal stem cells; Fetal therapy; Gastroschisis; Regenerative medicine