title: Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties.
abstract: Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Tharita
authors.initials: T
authors.lastname: Kitisripanya
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Kamonthip
authors.initials: K
authors.lastname: Jutathis
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Chadathorn
authors.initials: C
authors.lastname: Inyai
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Jukrapun
authors.initials: J
authors.lastname: Komaikul
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Orapin
authors.initials: O
authors.lastname: Udomsin
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
authors.forename: Gorawit
authors.initials: G
authors.lastname: Yusakul
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. waraporn@kku.ac.th.|Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand. waraporn@kku.ac.th.
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D000818:Animals; D000906:Antibodies; D007118:Immunoassay / Q000379:methods*; D008352:Mannich Bases; D010936:Plant Extracts / Q000276:immunology*; D029908:Pueraria / Q000737:chemistry*; D011817:Rabbits; D013256:Steroids / Q000032:analysis / Q000276:immunology*
chemical_list: D000906:Antibodies; D008352:Mannich Bases; D010936:Plant Extracts; D013256:Steroids; C571572:miroestrol
keywords: Immunoassay; Immunogen; Mannich reaction; Miroestrol; Pueraria candollei