title: Suppressor Screens in Arabidopsis.
abstract: Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Botany, University of British Columbia, Room #3529, 6270 University Boulevard, Vancouver, BC, Canada, V6T 1Z4. yuelin.zhang@ubc.ca.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015536:Down-Regulation; D005020:Ethyl Methanesulfonate; D005786:Gene Expression Regulation / Q000187:drug effects; D056726:Genetic Association Studies; D005820:Genetic Testing / Q000379:methods; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D005020:Ethyl Methanesulfonate
keywords: Arabidopsis; EMS mutagenesis; Suppressor screen