title: Identification and Characterization of Phytohemagglutinins from White Kidney Beans (Phaseolus vulgaris L., var. Beldia) in the Rat Small Intestine.
abstract: Although kidney bean (Phaseolus vulgaris L.) lectin toxicity is widely known, its effects in the gastrointestinal tract require further study. This investigation aimed to identify and characterize phytohemagglutinins (PHAs) in the small intestine and sera of rats following oral challenge with ground white beans. Twenty young, adult male rats were divided randomly into two groups of 10 animals each. The control group underwent gavage with a suspension of 300 mg of rodent pellet flour. The experimental group was administered a 300 mg Beldia bean flour suspension (BBFS). After 10 days of daily treatment, jejunal rinse liquid (JRL) and ileum rinse liquid and secretions, as well as sera, were collected. All biological fluids were screened for lectin reactivity using competitive inhibition ELISA, Ouchterlony double immunodiffusion, and immunoelectrophoresis techniques. The results revealed the presence of immunogenic intraluminal PHAs 3-4 h after the oral intake of the BBFS in the JRLs as well as in the jejunal and ileal secretions; however, no PHA was detectable in the rat sera. Ingestion of raw Beldia beans may lead to interaction between PHAs and the mucosa of the small intestine, potentially resulting in an inflammatory response.
authors.affiliation: 1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis, Tunisia .|2 Department of Animal Resources, Fisheries, and Food Technology, National Institute of Agronomy of Tunisia , Tunis, Tunisia .|3 School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education , Cheonan, Chungnam, Korea.
authors.forename: Nader
authors.initials: N
authors.lastname: Nciri
authors.affiliation: 3 School of Energy, Materials, and Chemical Engineering, Korea University of Technology and Education , Cheonan, Chungnam, Korea.
authors.forename: Namjun
authors.initials: N
authors.lastname: Cho
authors.affiliation: 1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis, Tunisia .
authors.forename: Faiçal
authors.initials: F
authors.lastname: El Mhamdi
authors.affiliation: 1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis, Tunisia .
authors.forename: Abderraouf
authors.initials: A
authors.lastname: Ben Mansour
authors.affiliation: 1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis, Tunisia .
authors.forename: Fayçal
authors.initials: F
authors.lastname: Haj Sassi
authors.affiliation: 1 Intestinal Immunophysiology-Research Unit (02/RU/09-02), Faculty of Medicine of Tunis, University of Tunis El Manar , Tunis, Tunisia .
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Aissa-Fennira
mesh_terms: D000818:Animals; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D027805:Phaseolus / Q000737:chemistry / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010835:Phytohemagglutinins
keywords: Phaseolus vulgaris L.; ileum; jejunum; phytohemagglutinins; white kidney beans