title: Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios.
abstract: The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n= 1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
authors.affiliation: Department of Chemistry, Xavier University of Louisiana, New Orleans LA 70125, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Manono
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Casey A
authors.initials: CA
authors.lastname: Dougherty
authors.affiliation: Department of Chemistry, Xavier University of Louisiana, New Orleans LA 70125, USA.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Jones
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: DeMuth
authors.affiliation: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Mark M Banaszak
authors.initials: MM
authors.lastname: Holl
authors.affiliation: Department of Chemistry, Xavier University of Louisiana, New Orleans LA 70125, USA.
authors.forename: Stassi
authors.initials: S
authors.lastname: DiMaggio
keywords: Fluorescent dye; PAMAM; Poly(amidoamine) dendrimer; rp-HPLC