title: Molecular characterization of Mycobacterium orygis isolates from wild animals of Nepal.
abstract: Mycobacterium orygis, a new member of the Mycobacterium tuberculosis complex, was isolated from a captive spotted deer (Axis axis) and a blue bull (Boselaphus tragocamelus) in Nepal. Analyses by spoligotyping, mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing, region of difference and single nucleotide polymorphism of genes gyrB, mmpL6, TbD1, PPE55 and Rv2042c confirmed the isolates as M. orygis. Moreover, analyses by spoligotyping (SIT587) as well as MIRU-VNTR showed that the isolates shared a similar pattern with many reported isolates. From previous and the present studies, it can be inferred that South Asia is one of the endemic regions for M. orygis. Further investigation including a larger sample size and different host interaction will help to understand the ecology and epidemiology of M. orygis in Nepal.
authors.forename: Jeewan
authors.initials: J
authors.lastname: Thapa
authors.forename: Chie
authors.initials: C
authors.lastname: Nakajima
authors.forename: Bhagwan
authors.initials: B
authors.lastname: Maharjan
authors.forename: Ajay
authors.initials: A
authors.lastname: Poudell
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D000866:Antelopes; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003670:Deer; D018598:Minisatellite Repeats; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D009164:Mycobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D009390:Nepal; D020641:Polymorphism, Single Nucleotide
chemical_list: D001426:Bacterial Proteins