title: In vitro synergistic effects of fisetin and norfloxacin against aquatic isolates of Serratia marcescens.
abstract: Serratia marcescens is a common pathogenic bacterium that can cause infections in both humans and animals. It can cause a range of diseases, from slight wound infections to life-threatening bacteraemia and pneumonia. The emergence of antimicrobial resistance has limited the treatment of the diseases caused by the bacterium to a great extent. Consequently, there is an urgent need to develop novel antimicrobial strategies against this pathogen. Synergistic strategy is a new approach to treat the infections caused by drug-resistant bacteria. In this paper, we isolated and identified the first multi-resistant pathogenic Serratia marcescens strain from diseased soft-shelled turtles (Pelodiscus sinensis) in China. We then performed a checkerboard assay; the results showed that out of 10 tested natural products fisetin had synergistic effects against S. marcescens when combined with norfloxacin. The time-kill curve assay further confirmed the results of the checkerboard assay. We found that this novel synergistic effect could significantly reduce the dosage of norfloxacin against S. marcescens.
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wudayuan Road 8#, Wuhan 430223, China Hubei Freshwater Aquaculture Collaborative Innovation Center, Shizishan Street 1#, Wuhan 430070, China dongjing@yfi.ac.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Donghu south Road 7#, Wuhan 430072, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ruan
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wudayuan Road 8#, Wuhan 430223, China Hubei Freshwater Aquaculture Collaborative Innovation Center, Shizishan Street 1#, Wuhan 430070, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wudayuan Road 8#, Wuhan 430223, China Hubei Freshwater Aquaculture Collaborative Innovation Center, Shizishan Street 1#, Wuhan 430070, China.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wudayuan Road 8#, Wuhan 430223, China Hubei Freshwater Aquaculture Collaborative Innovation Center, Shizishan Street 1#, Wuhan 430070, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Ai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D059001:Aquatic Organisms / Q000187:drug effects* / Q000302:isolation & purification; D002681:China; D004357:Drug Synergism; D005419:Flavonoids / Q000494:pharmacology*; D044948:Flavonols; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000494:pharmacology*; D016868:Serratia Infections / Q000382:microbiology / Q000662:veterinary; D012706:Serratia marcescens / Q000187:drug effects* / Q000302:isolation & purification; D014426:Turtles
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D044948:Flavonols; D009643:Norfloxacin; C017875:fisetin
keywords: Serratia marcescens; fisetin; resistance; synergism