title: Orthogonal optimization of prokaryotic expression of a natural snake venom phospholipase A2 inhibitor from Sinonatrix annularis.
abstract: Phospholipase A2 (PLA2) is a calcium-dependent enzyme that is involved in inflammatory processes such as the liberation of free arachidonic acid from the membrane pool for the biosynthesis of eicosanoids. Snake venom are known containing PLA2s (svPLA2s) which exhibit a wide variety of pharmacological effects including neurotoxicity, cardiotoxicity, myotoxicity and hemorrhage. Therefore, inhibition of svPLA2 would be advantageous to successful envenomation treatment. A gamma type PLI (PLA2 inhibitor) has been extracted from the serum of Sinonatrix annularis, a non-venomous snake indigenous to China. This showed strong inhibition of Deinagkistrrodon acutus PLA2, however, the PLIγ level in the serum and snake resource are not sufficiently sustainable for further research. To overcome these limitations, we constructed a His6-PLIγ pET28 fusion expression vector and transformed Escherichia coli BL21. To improve the expression of PLIγ, an orthogonal experiment [L16(4)(5)] was performed to optimize induction parameters. The optimized condition was determined to be: induction by 0.4 mM isopropyl-β-D-thiogalactoside (IPTG) for 6 h to the recombinant BL21 after its OD600 was 0.8, with continuous shaking cultivation at 190 rpm and 35 °C. Under these conditions, the amount of expressed protein could reach 57 mg/L. The His6-PLIγ was purified by nickel affinity chromatography and renatured by On-column refolding. The resulting PLIγ showed a good inhibitory effect of enzymatic activities to venom PLA2 isolated from D. acutus. Moreover, the PLIγ had a wide anti-hemorrhage activities to D. acutus, Naja atra and Agkistrodon halys venom.
authors.affiliation: Department of Biochemistry, College of Basic Medical Science, Nanchang University, Nanchang, 330006, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Le
authors.affiliation: Department of Biochemistry, College of Basic Medical Science, Nanchang University, Nanchang, 330006, China.
authors.forename: XingZhang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biochemistry, College of Basic Medical Science, Nanchang University, Nanchang, 330006, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yuan
authors.affiliation: First-Affiliated Hospital of Nanchang University, Nanchang, 330006, China.
authors.forename: Pi
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Biochemistry, College of Basic Medical Science, Nanchang University, Nanchang, 330006, China. Electronic address: merlynhuang@sohu.com.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Huang
mesh_terms: D000818:Animals; D002681:China; D017812:Colubridae / Q000097:blood*; D000066630:Ectopic Gene Expression; D004926:Escherichia coli / Q000235:genetics; D006490:Hemostatics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D008807:Mice, Inbred BALB C; D064801:Phospholipase A2 Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030162:Reptilian Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012910:Snake Venoms / Q000201:enzymology*; D017819:Viperidae / Q000378:metabolism
chemical_list: D006490:Hemostatics; D064801:Phospholipase A2 Inhibitors; D011993:Recombinant Fusion Proteins; D030162:Reptilian Proteins; D012910:Snake Venoms
keywords: Anti-venom PLA(2); Orthogonal test; PLIγ; Protein refolding