title: Charge-switching amino acids-based cationic lipids for efficient gene delivery.
abstract: A series of charge-switching amino acids-based cationic lipids 4a-4e bearing a benzyl ester at the terminus of the acyl chain, but differing in the polar-head group were prepared. The physicochemical properties of these lipids, including size, zeta potential and cellular uptake of the lipoplexes formed from with DNA, as well as the transfection efficiency (TE), were investigated. The results showed that the chemical structure of the cationic head-group clearly affects the physicochemical parameters of the amino acid-based lipids and especially the TE. The selected lipid, 4c gave 2.1 times higher TE than bPEI 25k in the presence of 10% serum in HeLa cells, with little toxicity.
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors.forename: Li-Ting
authors.initials: LT
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Yi
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China. Electronic address: lqchem@163.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China.
authors.forename: Rong-Chuan
authors.initials: RC
authors.lastname: Su
authors.affiliation: College of Chemistry and Environmental Protection Engineering, Southwest University for Nationalities, Chengdu 610041, PR China. Electronic address: zzg63129@163.com.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zhao
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002412:Cations / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D014162:Transfection / Q000379:methods*
chemical_list: D000596:Amino Acids; D002412:Cations; D008055:Lipids; D008081:Liposomes
keywords: Amino acids; Cationic lipid; Charge-switching; Non-viral vector; Serum