title: [A comparision of the effects of subcutaneous and sublingual immunotherapy on immunological responses in children with asthma].
abstract: OBJECTIVE
  To compare the difference in the effects of subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) on immunological responses in children with asthma.
  
  
  METHODS
  A total of 86 children with asthma caused by dust mites were enrolled and divided into a SLIT group (n=29), a SCIT group (n=13), a group receiving complete SCIT course (complete SCIT group; n=14), and a group receiving conventional medication (control group, n=30). Peripheral blood mononuclear cells were isolated and stimulated with house dust mite extract for 48 hours in vitro, and the percentage of regulatory T cells (Treg%) in CD4+ T cells was measured by flow cytometry. Analysis of variance with repeated measures was applied to compare the changes in humoral immunological indices and therapeutic effects in the SCIT and SLIT groups before treatment and after 6 and 12 months of treatment.
  
  
  RESULTS
  Before antigenic stimulation, Treg% in CD4(+) T cells in the SCIT group was significantly higher than that in the SLIT and control groups; after antigenic stimulation was given, Treg% in the four groups decreased significantly. After 6 and 12 months of immunotherapy, the SCIT group had significant changes in serum sIgE and sIgG4 levels, while the SLIT group only showed a significant change in serum sIgE level.
  
  
  CONCLUSIONS
  Temporal difference exists in different immunotherapies to cause immunological responses in children with asthma, and immunological responses induced by SCIT may occur earlier.
authors.affiliation: Allergy Department, Beijing Children's Hospital, Capital Medical University, 100045 Beijing, China. drxiangli@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Guan
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Liu
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Huang
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Ren
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong-Ge
authors.initials: YG
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000276:immunology / Q000628:therapy*; D002648:Child; D002675:Child, Preschool; D003888:Desensitization, Immunologic; D005260:Female; D006801:Humans; D008297:Male; D063729:Sublingual Immunotherapy