title: Immunocapture strategies in translational proteomics.
abstract: Aiming at clinical studies of human diseases, antibody-assisted assays have been applied to biomarker discovery and toward a streamlined translation from patient profiling to assays supporting personalized treatments. In recent years, integrated strategies to couple and combine antibodies with mass spectrometry-based proteomic efforts have emerged, allowing for novel possibilities in basic and clinical research. Described in this review are some of the field's current and emerging immunocapture approaches from an affinity proteomics perspective. Discussed are some of their advantages, pitfalls and opportunities for the next phase in clinical and translational proteomics.
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fredolini
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Sanna
authors.initials: S
authors.lastname: Byström
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Elisa
authors.initials: E
authors.lastname: Pin
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Edfors
authors.affiliation: b Department of Oncology-Pathology, Clinical Proteomics Mass Spectrometry, SciLifeLab , Karolinska Institutet , Solna , Sweden.
authors.forename: Davide
authors.initials: D
authors.lastname: Tamburro
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Maria Jesus
authors.initials: MJ
authors.lastname: Iglesias
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Häggmark
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Mun-Gwan
authors.initials: MG
authors.lastname: Hong
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlen
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.affiliation: a Affinity Proteomics, SciLifeLab, School of Biotechnology , KTH - Royal Institute of Technology , Solna , Sweden.
authors.forename: Jochen M
authors.initials: JM
authors.lastname: Schwenk
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D006801:Humans; D047468:Immunoprecipitation; D057230:Limit of Detection; D013058:Mass Spectrometry; D011485:Protein Binding; D020543:Proteome / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods; D057170:Translational Research, Biomedical
chemical_list: D000906:Antibodies; D020543:Proteome
keywords: Antibodies; SRM; immunocapture; mass spectrometry; protein enrichment; sandwich assays