title: Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.
abstract: During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Ji-Hyeon
authors.initials: JH
authors.lastname: Hwang
authors.affiliation: Enzynomics, Inc., Daejeon, Republic of Korea.
authors.forename: Yong-Keol
authors.initials: YK
authors.lastname: Shin
authors.affiliation: Enzynomics, Inc., Daejeon, Republic of Korea.
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Park
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Jeesoo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Su-Mi
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Byounghan
authors.initials: B
authors.lastname: Kim
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
authors.affiliation: Veterinary College, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Foot and Mouth Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea leekwn@korea.kr.
authors.forename: Kwang-Nyeong
authors.initials: KN
authors.lastname: Lee
mesh_terms: D000818:Animals; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D003666:Decontamination / Q000379:methods*; D004866:Equipment Contamination; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000453:epidemiology; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004262:DNA Restriction Enzymes