title: Phage display screening identifies a novel peptide to suppress ovarian cancer cells in vitro and in vivo in mouse models.
abstract: BACKGROUND
  Ovarian cancer is a possibly lethal gynecological malignancy and this study utilized phage display technology to screen and identify peptides that specifically bind to ovarian cancer cells and explored the effects of these peptides on ovarian cancer cells in vitro and in vivo.
  
  
  METHODS
  The phage displayed peptide library was used to isolate the peptides binding to and internalizing into the ovarian carcinoma cells. Positive phage clones were characterized with DNA sequencing and bioinformatics analysis and then validated with immunofluorescence. Subsequently, the selected peptides were investigated for their cancer-related functions, including cell adhesion, spreading, motility, and invasion in vitro and in vivo.
  
  
  RESULTS
  Peptide1 read as SWQIGGNwas the positive peptide and showed preferential binding to the target cells. Peptide 1 also inhibited cell proliferation, migration, invasion and adhesion of ovarian cancer HO8910 cells in vitro. In vivo, Peptide 1 led to a lower tumorigenicity of HO8910 cells, which was characterized by the inhibitory effect on tumor growth and metastasis of ovarian cells.
  
  
  CONCLUSIONS
  These studies demonstrate that the phage display-identified tumor cell-binding peptide was able to control ovarian cancer cell viability, migration, invasion, and adhesion capacity in vitro as well as tumor growth and metastasis in vivo. Future studies will be aimed at evaluating the clinical efficacy of the peptide SWQIGGN in ovarian cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, Maternity and Children's Healthcare Hospital of Foshan, Foshan, 528000, Guangdong, China. congcongcn2@126.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China. kangjiali1961@21cn.com.
authors.forename: Jiali
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China. wxx605@sohu.com.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Foshan Hospital of TCM, Foushan, 52800, China. Weiweicn2@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Obstetrics and Gynecology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China. jiangwenyan_@126.com.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D061505:Cell Surface Display Techniques; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism / Q000627:therapeutic use*; D011485:Protein Binding
chemical_list: D019151:Peptide Library; D010455:Peptides