title: EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.
abstract: BACKGROUND
  The addition of the detergent Orvus ES Paste (OEP) to semen freezing extenders has been observed to improve the post-thaw survival and longevity of spermatozoa from various species but has never been evaluated for yak spermatozoa.
  
  
  OBJECTIVE
  This study evaluated the effects of OEP on the post-thaw motility and viability of epididymal and ejaculated yak spermatozoa.
  
  
  METHODS
  Semen samples were frozen and thawed in semen freezing extender supplemented with 0 %, 0.375 %, 0.75 % or 1.5 % OEP. The motility and viability of frozen-thawed spermatozoa were evaluated before and after 3 h of incubation.
  
  
  RESULTS
  The addition of 0.75 % OEP to the freezing extender significantly improved the mean motility and viability values of both the epididymal and ejaculated spermatozoa immediately after thawing, but the beneficial effects on motility disappeared after 3h of incubation.
  
  
  CONCLUSIONS
  Our findings indicate that the addition of 0.75 % OEP is effective for the preservation of yak spermatozoa.
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, JapanYamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shimazaki
authors.affiliation: Institute for Extension of Agricultural Advanced Technology, Ulaanbaatar, Mongolia.
authors.forename: R
authors.initials: R
authors.lastname: Sambuu
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: L T
authors.initials: LT
authors.lastname: Kim Do
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Tanihara
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Department of Animal Reproduction, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan. otoi@yamaguchi-u.ac.jp.
authors.forename: T
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology*; D004822:Epididymis / Q000166:cytology / Q000187:drug effects; D005615:Freezing; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D003902:Detergents