title: Association of Tumor Necrosis Factor Alpha 308 G/A Polymorphism with Asthma in Pakistani Population.
abstract: Asthma is a chronic inflammatory and remodeling disorder of the airways, in which many cells, cellular elements, and cytokines play important roles. The role of tumor necrosis factor-α (TNF-α) in asthma is unclear in Pakistani population. The aim of this study was to assess the relationship between TNF-α-308 polymorphism and asthma. Polymorphism of TNF-α (G-308-A locus; rs 1800629) in 329 asthmatic patients and 151 healthy controls was evaluated. DNA was prepared from blood samples of cases and controls. Samples were genotyped for TNF-α 308 G/A polymorphism. There was no significant difference in the frequency of GG (OR 1.049 with 95% CI 0.68-1.63) and GA (OR 0.987 with 95% CI 0.64-1.53) genotypes of TNF-α-308. The AA genotype was absent in cases and only one AA genotype was observed in the controls. The genetic polymorphism of TNF-α does not seem to be associated with asthma in Pakistani population.
authors.affiliation: Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan AND Department of Biochemistry, PirMehar Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Saba
authors.affiliation: The Allergy and Asthma Institute of Pakistan, Islamabad, Pakistan.
authors.forename: Osman
authors.initials: O
authors.lastname: Yusuf
authors.affiliation: Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
authors.forename: Sadia
authors.initials: S
authors.lastname: Rehman
authors.affiliation: Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
authors.forename: Saeeda
authors.initials: S
authors.lastname: Munir
authors.affiliation: Chest and Medical Specialist, MEDICSI, Islamabad, Pakistan.
authors.forename: Naghman
authors.initials: N
authors.lastname: Bashir
authors.affiliation: Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan.
authors.forename: Atika
authors.initials: A
authors.lastname: Mansoor
authors.affiliation: Department of Biochemistry, PirMehar Ali Shah Arid Agriculture University Rawalpindi, Pakistan.
authors.forename: Ghazala
authors.initials: G
authors.lastname: Raja Kaukab
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001249:Asthma / Q000235:genetics*; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha
keywords: Asthma; Pakistani population; TNF alpha 308