title: Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin.
abstract: While screening for new antimicrobial agents for multidrug-resistant Salmonella enterica, the novel lytic bacteriophage STP4-a was isolated and characterized. Phage morphology revealed that STP4-a belongs to the family Myoviridae. Bacterial challenge assays showed that different serovars of Salmonella enterica were susceptible to STP4-a infection. The genomic characteristics of STP4-a, containing 159,914 bp of dsDNA with an average GC content of 36.86 %, were determined. Furthermore, the endolysin of STP4-a was expressed and characterized. The novel endolysin, LysSTP4, has hydrolytic activity towards outer-membrane-permeabilized S. enterica and Escherichia coli. These results provide essential information for the development of novel phage-based biocontrol agents against S. enterica.
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Mengzhe
authors.initials: M
authors.lastname: Li
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China. snow@ouc.edu.cn.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Food Safety Laboratory, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Han
mesh_terms: D001433:Bacteriolysis; D001482:Base Composition; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D010450:Endopeptidases; D004926:Escherichia coli / Q000187:drug effects; D023061:Gene Order; D016679:Genome, Viral; D058507:Host Specificity; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D012483:Salmonella Phages / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D019779:Salmonella enterica / Q000187:drug effects / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral; D010450:Endopeptidases; C001143:endolysin