title: Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins.
abstract: Glycosylation is one of major modifications for plant secondary metabolites. In the case of volatile compounds, glycosylation makes them nonvolatile and odorless. Identification of UDP-dependent glycosyltransferases responsible for volatile glycosylation is essential to understand the regulatory mechanism of volatile release from plant tissues. Here, we describe an efficient protocol to find possible combinations of volatiles/glycosyltransferases using tomato (Solanum lycopersicum) enzymes expressed in Escherichia coli. The presented method requires a basic gas chromatography system and conventional laboratory tools.
authors.affiliation: Horticultural Sciences Department, University of Florida, Building 885, Gainesville, FL, 32611-0690, USA.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kamiyoshihara
authors.affiliation: Horticultural Sciences Department, University of Florida, Building 885, Gainesville, FL, 32611-0690, USA.
authors.forename: Denise M
authors.initials: DM
authors.lastname: Tieman
authors.affiliation: Horticultural Sciences Department, University of Florida, Building 885, Gainesville, FL, 32611-0690, USA. hjklee@ufl.edu.
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Klee
mesh_terms: D002849:Chromatography, Gas; D006027:Glycosides; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014530:Uridine Diphosphate / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism*
chemical_list: D006027:Glycosides; D011994:Recombinant Proteins; D055549:Volatile Organic Compounds; D014530:Uridine Diphosphate; D016695:Glycosyltransferases
keywords: Escherichia coli; Gas chromatography; Glycosides; Screening; UGT; Volatiles