title: Apta-PCR.
abstract: Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers. Ultra-low detection of target analyte can potentially be achieved, or, improved detection limits can be achieved with aptamers of low-medium affinity. Herein, we describe a generic methodology coined real-time Apta-PCR, using a model target (β-conglutin) and a competitive format, which can be adapted for the detection of any target which an aptamer has been selected for.
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain.|Department of Bioengineering, Rice University, 6500 Main Street, 77030, Houston, TX, USA.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Pinto
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain.|Center for Omic Sciences (COS), Universitat Rovira I Virgili, Avinguda Universitat n° 1, 43204, Reus, Spain.
authors.forename: Pedro Nadal
authors.initials: PN
authors.lastname: Polo
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain.
authors.forename: Miriam Jauest
authors.initials: MJ
authors.lastname: Rubio
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain.
authors.forename: Marketa
authors.initials: M
authors.lastname: Svobodova
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain.
authors.forename: Teresa Mairal
authors.initials: TM
authors.lastname: Lerga
authors.affiliation: Department of Chemical Engineering, Universitat Rovira I Virgili, Avinguda Päısos Catalans 26, Tarragona, 43007, Spain. ciara.osullivan@urv.cat.|Institució Catalana de Recerca I Estudis Avançats, Passeig Llúıs Companys 23, 08010, Barcelona, Spain. ciara.osullivan@urv.cat.
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D052156:SELEX Aptamer Technique; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide
keywords: Apta-PCR; Aptamer; Protein quantification