title: A Systems Biology-Based Investigation into the Pharmacological Mechanisms of Sheng-ma-bie-jia-tang Acting on Systemic Lupus Erythematosus by Multi-Level Data Integration.
abstract: Sheng-ma-bie-jia-tang (SMBJT) is a Traditional Chinese Medicine (TCM) formula that is widely used for the treatment of Systemic Lupus Erythematosus (SLE) in China. However, molecular mechanism behind this formula remains unknown. Here, we systematically analyzed targets of the ingredients in SMBJT to evaluate its potential molecular mechanism. First, we collected 1,267 targets from our previously published database, the Traditional Chinese Medicine Integrated Database (TCMID). Next, we conducted gene ontology and pathway enrichment analyses for these targets and determined that they were enriched in metabolism (amino acids, fatty acids, etc.) and signaling pathways (chemokines, Toll-like receptors, adipocytokines, etc.). 96 targets, which are known SLE disease proteins, were identified as essential targets and the rest 1,171 targets were defined as common targets of this formula. The essential targets directly interacted with SLE disease proteins. Besides, some common targets also had essential connections to both key targets and SLE disease proteins in enriched signaling pathway, e.g. toll-like receptor signaling pathway. We also found distinct function of essential and common targets in immune system processes. This multi-level approach to deciphering the underlying mechanism of SMBJT treatment of SLE details a new perspective that will further our understanding of TCM formulas.
authors.affiliation: TCM Clinical Basis Institute, Zhejiang Chinese Medicine University, 548 Binwen Road, Hangzhou, Zhejiang, 310000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: Center for Bioinformatics and Computational Biology, and the Institute of Biomedical Sciences, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.|School of Finance and Statistics, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lv
authors.affiliation: TCM Clinical Basis Institute, Zhejiang Chinese Medicine University, 548 Binwen Road, Hangzhou, Zhejiang, 310000, China.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Liu
authors.affiliation: Center for Bioinformatics and Computational Biology, and the Institute of Biomedical Sciences, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, China.|Biological Targeting Diagnosis and Therapy Research Center, Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Tieliu
authors.initials: T
authors.lastname: Shi
authors.affiliation: TCM Clinical Basis Institute, Zhejiang Chinese Medicine University, 548 Binwen Road, Hangzhou, Zhejiang, 310000, China.
authors.forename: Chengping
authors.initials: C
authors.lastname: Wen
mesh_terms: D002626:Chemistry, Pharmaceutical; D019295:Computational Biology / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D063990:Gene Ontology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D015398:Signal Transduction / Q000187:drug effects; D049490:Systems Biology / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal