title: A and D genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant.
abstract: Chromosomal dispositions were analyzed on the metaphase plate of tetraploid cotton (AADD). At metaphase, the two subgenomes, A and D, were separated in a radial pattern in which the small D subgenome chromosomes tended to concentrate at the center and the large A subgenome chromosomes were scattered about the periphery on the metaphase plate. Although the ordered chromosome arrangement was disturbed in an artificial hexaploid (AADDGG), the separation pattern could be recovered after the majority of the additional genome (GG) chromosomes were removed by backcrossing the artificial hexaploid with the tetraploid cotton (AADD). A similar genome separation phenomenon was also found in synthesized tetraploid cotton (AAGG). These results indicate that the genome separation pattern could be established immediately after tetraploid cotton formation and could be stably inherited in tetraploid cotton. Given the evidence of parental genome separation in other plants and animals, we speculated that genome separation might be a normal phenomenon in diploid and polyploid species. These finding will shed light on the chromosome conformation in plant cells.
authors.affiliation: Center for Genomics and Biotechnology, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Jinlei
authors.initials: J
authors.lastname: Han
authors.affiliation: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Baoliang
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Center for Genomics and Biotechnology, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Shan
authors.affiliation: Center for Genomics and Biotechnology, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Weiren
authors.initials: W
authors.lastname: Wu
authors.affiliation: Center for Genomics and Biotechnology, Haixia Institute of Science and Technology (HIST), Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
mesh_terms: D032461:Chromosomes, Plant; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D008677:Metaphase / Q000502:physiology*; D057891:Tetraploidy
keywords: Gossypium hirsutum; genome separation; metaphase chromosome; radial pattern; synthesized cotton