title: Th17 master transcription factors RORα and RORγ regulate the expression of IL-17C, IL-17D and IL-17F in Cynoglossus semilaevis.
abstract: The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors. In this study, we examined the regulatory properties of RORα (CsRORα) and RORγ (CsRORγ) in tongue sole (Cynoglossus semilaevis). CsRORα and CsRORγ expression was detected in major lymphoid organs and altered to significant extents after bacterial and viral infection. CsRORα enhanced the activities of CsIL-17C, CsIL-17D, and CsIL-17F promoters, which contain CsRORα and CsRORγ binding sites. CsRORγ also upregulated the promoter activities of CsIL-17D and CsIL-17F but not CsIL-17C. CsRORα and CsRORγ proteins were detected in the nucleus, and overexpression of CsRORα in tongue sole significantly increased the expression of CsIL-17C, CsIL-17D, and CsIL-17F, whereas overexpression of CsRORγ significantly increased the expression of CsIL-17C and CsIL-17F but no CsIL-17D. These results indicate that RORα and RORγ in teleost regulate the expression of IL-17 members in different manners.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Chi
authors.affiliation: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, Tromsø N-9037, Norway.
authors.forename: Jarl
authors.initials: J
authors.lastname: Bøgwald
authors.affiliation: Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, Tromsø N-9037, Norway.
authors.forename: Roy Ambli
authors.initials: RA
authors.lastname: Dalmo
authors.affiliation: College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: huyonghua@qdio.ac.cn.
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004266:DNA Virus Infections / Q000276:immunology*; D005412:Flatfishes / Q000276:immunology*; D005786:Gene Expression Regulation; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D064094:Interleukin-27 / Q000235:genetics / Q000378:metabolism; D007497:Iridoviridae / Q000276:immunology*; D008969:Molecular Sequence Data; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D058504:Th17 Cells / Q000276:immunology; D019076:Transgenes / Q000235:genetics; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D020381:Interleukin-17; D064094:Interleukin-27; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D018160:Receptors, Cytoplasmic and Nuclear; D029961:Zebrafish Proteins; C521431:roraa protein, zebrafish
keywords: Cynoglossus semilaevis; IL-17; Promoter activity; RORα; RORγ