title: The application of HPLC-MS/MS to studies of pharmacokinetics and interconversion of isoliquiritigenin and neoisoliquiritigenin in rats.
abstract: A specific and sensitive HPLC-MS/MS method was developed and validated for the simultaneously quantification of isoliquiritigenin (ISL) and neoisoliquiritin (NIS) in rat plasma by oral administration. Analytes were analyzed on an Agilent 6460 LC-MS/MS system (Agilent, USA) using an Agilent Zorbax SB-C18 column (4.6 × 150 mm, 5 μm). Gradient elution was applied for the analyte separation using a mobile phase composed of 0.1% formic acid aqueous solution and methanol at a flow rate of 1.0 mL/min with a total running time of 12 min. The calibration curves for ISL and NIS showed good linearity in the concentrations ranging from 0.001 to 4.000 μg/mL with correlation coefficients >0.998. The precision, accuracy, recovery and stability were deemed acceptable. The method was applied to the pharmacokinetics study of ISL and NIS in rats by single and combination administration. The result showed that Cmax and AUC0→t of ISL were markedly increased from 0.53 to 1.20 μg/mL, and from 69.63 to 200.74 min μg/mL by combination administration. The mean t1/2 value was also prolonged from 64.55 to 203.74 min in the combination group. These results indicated that NIS may have been metabolized to ISL which increased the absorption and extended the elimination of ISL. However, little difference was found for NIS pharmacokinetics parameters between single NIS and the combination group, which suggested that there was no significant biotransformation of ISL to NIS. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
authors.forename: Fu
authors.initials: F
authors.lastname: Peng
authors.affiliation: Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine; State Key Laboratory Breeding Base of Systematic Research, People's Republic of China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Gong
authors.affiliation: Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine; State Key Laboratory Breeding Base of Systematic Research, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiong
authors.affiliation: School of Chinese Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Chen
authors.affiliation: Development and Utilization of Chinese Medicine Resources, Pharmacy College, Chengdu University of Traditional Chinese Medicine; State Key Laboratory Breeding Base of Systematic Research, People's Republic of China.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Li
mesh_terms: D000818:Animals; D002599:Chalcone / Q000031:analogs & derivatives* / Q000097:blood; D047188:Chalcones / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047188:Chalcones; D005960:Glucosides; C098467:neoisoliquiritin; D002599:Chalcone; C040920:isoliquiritigenin
keywords: HPLC-MS/MS; interconversion; isoliquiritigenin; neoisoliquiritin; pharmacokinetics