title: Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.
abstract: A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.
authors.affiliation: International Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: Fangping
authors.initials: F
authors.lastname: Cai
authors.affiliation: Microbiology Department, National Autonomous University of Honduras, Tegucigalpa, Honduras.
authors.forename: Ivette Lorenzana
authors.initials: IL
authors.lastname: de Rivera
authors.affiliation: International Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: International Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: International Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA.
authors.forename: John
authors.initials: J
authors.lastname: Nkengasong
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA cyang1@cdc.gov fgao@duke.edu.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: International Laboratory Branch, Division of Global HIV/AIDS, Center for Global Health, CDC, Atlanta, Georgia, USA cyang1@cdc.gov fgao@duke.edu.
authors.forename: Chunfu
authors.initials: C
authors.lastname: Yang
mesh_terms: D001769:Blood / Q000821:virology*; D003890:Desiccation; D024882:Drug Resistance, Viral; D060005:Genotyping Techniques / Q000379:methods*; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D020125:Mutation, Missense; D013048:Specimen Handling / Q000379:methods*