title: Effect of the Surface Charge of Artificial Chaperones on the Refolding of Thermally Denatured Lysozymes.
abstract: Artificial chaperones are of great interest in fighting protein misfolding and aggregation for the protection of protein bioactivity. A comprehensive understanding of the interaction between artificial chaperones and proteins is critical for the effective utilization of these materials in biomedicine. In this work, we fabricated three kinds of artificial chaperones with different surface charges based on mixed-shell polymeric micelles (MSPMs), and investigated their protective effect for lysozymes under thermal stress. It was found that MSPMs with different surface charges showed distinct chaperone-like behavior, and the neutral MSPM with PEG shell and PMEO2MA hydrophobic domain at high temperature is superior to the negatively and positively charged one, because of the excessive electrostatic interactions between the protein and charged MSPMs. The results may benefit to optimize this kind of artificial chaperone with enhanced properties and expand their application in the future.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Jianzu
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Aoting
authors.initials: A
authors.lastname: Qu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Huiru
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: An
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University , Tianjin 300071, China.
authors.forename: Linqi
authors.initials: L
authors.lastname: Shi
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000737:chemistry*; D002645:Chickens; D006358:Hot Temperature; D006801:Humans; D008823:Micelles; D018832:Molecular Chaperones / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011489:Protein Denaturation; D058849:Protein Refolding
chemical_list: D030161:Avian Proteins; D008823:Micelles; D018832:Molecular Chaperones; D011092:Polyethylene Glycols; D009113:Muramidase; C000595209:polyethylene glycol 1000
keywords: artificial chaperones; mixed-shell polymeric micelle; protein folding; self-assembly; surface charge