title: The protective effect of intraperitoneal medical ozone preconditioning and treatment on hepatotoxicity induced by methotrexate.
abstract: The aim of this study is to determine the effects of medical ozone preconditioning and treatment on the methotrexate acute induced hepatotoxicity in rats that has not reports elsewhere. Eighteen rats were randomly assigned into three equal groups; control, Mtx and Mtx with ozone. Hepatotoxicity was performed with a single dose of 20 mg/kg Mtx to group 2 and group 3 at the fifteenth day. The medical ozone preconditioning was administered intraperitonealy in group 3 for fifteen days and more five days after inducing Mtx. The other rats of the group 1 and 2 received saline injection. At the twentyfirst day the blood and the liver tissue samples were obtained to measure the levels of liver enzymes ALT and AST, proinflamatory cytokines TNF-α, IL-1β, malondialdehyde, glutathione and myeloperoxidase. And the histolopatological examination was evaluated for injury score. In our study Mtx administration caused a significant increase on the liver enzymes ALT and AST, the tissue MDA and MPO activity and significant decrease in the tissue GSH. Moreover the both pro-inflammatory cytokines were significantly increased in the Mtx group. Medical ozone preconditioning and treatment reversed all these biochemical parameters and histopathological changes of the hepatotoxicity induced by Mtx. We conclude that medical ozone ameliorates Mtx induced hepatotoxicity in rats.
authors.affiliation: Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Arif
authors.initials: A
authors.lastname: Aslaner
authors.affiliation: Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Tuğrul
authors.initials: T
authors.lastname: Çakır
authors.affiliation: Department of Pathology, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Betül
authors.initials: B
authors.lastname: Çelik
authors.affiliation: Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Doğan
authors.affiliation: Department of Gastroenterologic Surgery, Haydarpaşa Training and Research Hospital Istanbul, Turkey.
authors.forename: Cebrail
authors.initials: C
authors.lastname: Akyüz
authors.affiliation: Department of Pediatric Gastroenterology, Akdeniz University Antalya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Baştürk
authors.affiliation: Department of Biochemistry, Public Health Laboratuary Kütahya, Turkey.
authors.forename: Cemal
authors.initials: C
authors.lastname: Polat
authors.affiliation: Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Gündüz
authors.affiliation: Department of General Surgery, Antalya Education and Research Hospital Antalya, Turkey.
authors.forename: Burhan
authors.initials: B
authors.lastname: Mayir
authors.affiliation: Department of Pharmacology, Marmara University Istanbul, Turkey ; Near East University Faculty of Denstry Nicosia, North Cyprus.
authors.forename: Ahmet Özer
authors.initials: AÖ
authors.lastname: Şehirli
keywords: Methotrexate; hepatotoxicity; liver; medical ozone; preconditioning