title: Preparation, characterization, and biocompatibility evaluation of poly(Nɛ-acryloyl-L-lysine)/hyaluronic acid interpenetrating network hydrogels.
abstract: In the present study, poly(Nɛ-acryloyl-L-lysine)/hyaluronic acid (pLysAAm/HA) interpenetrating network (IPN) hydrogels were successfully fabricated through the combination of hydrazone bond crosslinking and photo-crosslinking reactions. The HA hydrogel network was first synthesized from 3,3'-dithiodipropionate hydrazide-modified HA and polyethylene glycol dilevulinate by hydrazone bond crosslinking. The pLysAAm hydrogel network was prepared from Nɛ-acryloyl-L-lysine and N,N'-bis(acryloyl)-(L)-cystine by photo-crosslinking. The resultant pLysAAm/HA hydrogels had a good shape recovery property after loading and unloading for 1.5 cycles (up to 90%) and displayed a highly porous microstructure. Their compressive moduli were at least 5 times higher than that of HA hydrogels. The pLysAAm/HA hydrogels had an equilibrium swelling ratio of up to 37.9 and displayed a glutathione-responsive degradation behavior. The results from in vitro biocompatibility evaluation with pre-osteoblasts MC3T3-E1 cells revealed that the pLysAAm/HA hydrogels could support cell viability and proliferation. Hematoxylin and eosin staining indicated that the pLysAAm/HA hydrogels allowed cell and tissue infiltration, confirming their good in vivo biocompatibility. Therefore, the novel pLysAAm/HA IPN hydrogels have great potential for bone tissue engineering applications.
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: jmqian@mail.xjtu.edu.cn.
authors.forename: Junmin
authors.initials: J
authors.lastname: Qian
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D019245:Compressive Strength; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D020100:Hydrogels / Q000009:adverse effects / Q000737:chemistry*; D008239:Lysine / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D020100:Hydrogels; D006820:Hyaluronic Acid; D008239:Lysine
keywords: Biomaterials; Bone tissue engineering; Hyaluronic acid; Hydrogel; Poly(N(ɛ)-acryloyl-l-lysine)