title: Formulation and in vivo evaluation of probiotics-encapsulated pellets with hydroxypropyl methylcellulose acetate succinate (HPMCAS).
abstract: The aim of this study was to formulate probiotics-encapsulated pellets with hydroxypropyl methylcellulose acetate succinate (HPMCAS) using a dry powder coating technique to improve the storage stability, acid resistance, and intestinal adherence of viable bacteria (Lactobacillus acidophilus and Bifidobacteria animalis ssp. Lactis). Dry coated pellet (DCP) loaded with probiotics was optimized with respect to the quantity of the HPMCAS, an enteric coating polymer (108 mg), and the kinds and amounts of plasticizer (triethyl citrate, 15.7 mg; acetylated monoglyceride, 6.8 mg), by evaluating the survival rate of the bacteria during preparation process and in an acidic medium. Dry coating process allows the whole survivals of living bacteria during preparation process. The DCP formulation exhibited markedly higher acid tolerability and storage stability compared to uncoated viable bacteria. In an in vivo mucosal adherence study in rats, a profound colonization of viable bacteria in the small and large intestine was observed in rats receiving DCP system (p<0.05) compared to rats receiving uncoated probiotics. Moreover, we found that the repeated DCP administration noticeably inhibited intestinal penetration of endotoxin, a potent inflammatory stimulant, from intestinal mucus. The novel DCP system may be an alternative approach for improving bacterial viability in the preparation process and in an acidic medium, and to promote mucosal colonization of probiotic bacteria in the human gut.
authors.affiliation: Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories, Giheung-gu, Yongin, Gyeonggi 446-905, Korea.
authors.forename: Hee Jun
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories, Giheung-gu, Yongin, Gyeonggi 446-905, Korea.
authors.forename: Ga Hyeon
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories, Giheung-gu, Yongin, Gyeonggi 446-905, Korea.
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Jun
authors.affiliation: Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories, Giheung-gu, Yongin, Gyeonggi 446-905, Korea.
authors.forename: Miwon
authors.initials: M
authors.lastname: Son
authors.affiliation: College of Pharmacy, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam 330-714, Korea.
authors.forename: Yong Seok
authors.initials: YS
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam 330-714, Korea.
authors.forename: Min-Koo
authors.initials: MK
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Dankook University, 119 Dandae-ro, Dongnam-gu, Cheonan, Chungnam 330-714, Korea. Electronic address: kangmj@dankook.ac.kr.
authors.forename: Myung Joo
authors.initials: MJ
authors.lastname: Kang
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000187:drug effects / Q000472:pathogenicity; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000472:pathogenicity; D008297:Male; D008747:Methylcellulose / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000494:pharmacology; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C048331:hydroxypropylmethylcellulose acetate succinate; D008747:Methylcellulose
keywords: Acid resistance; Dry powder coating technique; HPMCAS; Intestinal adherence; Probiotics