title: Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry.
abstract: Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.
authors.affiliation: Trisaia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), 75026, Rotondella, MT, Italy. elio.fantini@enea.it.
authors.forename: Elio
authors.initials: E
authors.lastname: Fantini
authors.affiliation: Casaccia Research Center, ENEA (Italian National Agency for New Technologies, Energy, and Sustainable Development), via Anguillarese 301, 00123, Rome, Italy. giovanni.giuliano@enea.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Giuliano
mesh_terms: D016960:Agrobacterium tumefaciens; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism*; D010942:Plant Viruses / Q000235:genetics*; D034622:RNA Interference
keywords: Reverse genetics; Silencing; TRV; Tomato; VIGS