title: The RING Finger Ubiquitin E3 Ligase OsHTAS Enhances Heat Tolerance by Promoting H2O2-Induced Stomatal Closure in Rice.
abstract: Heat stress often results in the generation of reactive oxygen species, such as hydrogen peroxide, which plays a vital role as a secondary messenger in the process of abscisic acid (ABA)-mediated stomatal closure. Here, we characterized the rice (Oryza sativa) HEAT TOLERANCE AT SEEDLING STAGE (OsHTAS) gene, which plays a positive role in heat tolerance at the seedling stage. OsHTAS encodes a ubiquitin ligase localized to the nucleus and cytoplasm. OsHTAS expression was detected in all tissues surveyed and peaked in leaf blade, in which the expression was concentrated in mesophyll cells. OsHTAS was responsive to multiple stresses and was strongly induced by exogenous ABA. In yeast two-hybrid assays, OsHTAS interacted with components of the ubiquitin/26S proteasome system and an isoform of rice ascorbate peroxidase. OsHTAS modulated hydrogen peroxide accumulation in shoots, altered the stomatal aperture status of rice leaves, and promoted ABA biosynthesis. The results suggested that the RING finger ubiquitin E3 ligase OsHTAS functions in leaf blade to enhance heat tolerance through modulation of hydrogen peroxide-induced stomatal closure and is involved in both ABA-dependent and DROUGHT AND SALT TOLERANCE-mediated pathways.
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Jianping
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.) zhangcuicui@zju.edu.cn jtu@zju.edu.cn.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Chuchu
authors.initials: C
authors.lastname: Wei
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Mugui
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Feifei
authors.initials: F
authors.lastname: Yu
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.).
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
authors.affiliation: Institute of Crop Science, Zhejiang University, Hangzhou 310058, China (J.L., C.Z., C.W., X.L., M.W., J.T.); andState Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China (F.Y., Q.X.) zhangcuicui@zju.edu.cn jtu@zju.edu.cn.
authors.forename: Jumin
authors.initials: J
authors.lastname: Tu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D012275:Oryza / Q000502:physiology*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D054046:Plant Stomata / Q000502:physiology*; D030821:Plants, Genetically Modified; D054829:RING Finger Domains; D036226:Seedlings / Q000502:physiology; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D025801:Ubiquitin; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D044767:Ubiquitin-Protein Ligases