title: Biocompatible Mesoporous Nanotubular Structured Surface to Control Cell Behaviors and Deliver Bioactive Molecules.
abstract: Biocompatible nanostructured surfaces control the cell behaviors and tissue integration process of medical devices and implants. Here we develop a novel biocompatible nanostructured surface based on mesoporous silica nanotube (MSNT) by means of an electrodeposition. MSNTs, replicated from carbon nanotubes of 25 nm × 1200 nm size, were interfaced in combination with fugitive biopolymers (chitosan or collagen) onto a Ti metallic substrate. The MSNT-biopolymer deposits uniformly covered the substrate with weight gains controllable by the electrodeposition conditions. Random nanotubular networks were generated successfully, which alongside the high mesoporosity provided unique nanotopological properties for the cell responses and the loading/delivery of biomolecules. Of note, the adhesion and spreading behaviors of mesenchymal stem cells (MSCs) were significantly altered, revealing more rapid cell anchorage and extensive nanofilopodia development along the nanotubular networks. Furthermore, the nanotubular surface improved the loading capacity of biomolecules (dexamethasone and bovine serum albumin) up to 5-7 times. The release of the biomolecules was highly sustained, exhibiting a diffusion-controlled pattern over 15 days. The therapeutic efficacy of the delivered biomolecules was also confirmed in the osteogenic differentiation of MSCs. While in vivo performance and applicability studies are needed further, the current biocompatible nanostructured surface may be considered as a novel biointerfacing platform to control cellular behaviors and biomolecular delivery.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), ‡Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, and §Department of Biomaterials Science, School of Dentistry, Dankook University , Cheonan 330-714, South Korea.
authors.forename: Kapil D
authors.initials: KD
authors.lastname: Patel
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), ‡Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, and §Department of Biomaterials Science, School of Dentistry, Dankook University , Cheonan 330-714, South Korea.
authors.forename: Chinmaya
authors.initials: C
authors.lastname: Mahapatra
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), ‡Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, and §Department of Biomaterials Science, School of Dentistry, Dankook University , Cheonan 330-714, South Korea.
authors.forename: Guang-Zhen
authors.initials: GZ
authors.lastname: Jin
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), ‡Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, and §Department of Biomaterials Science, School of Dentistry, Dankook University , Cheonan 330-714, South Korea.
authors.forename: Rajendra K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), ‡Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, and §Department of Biomaterials Science, School of Dentistry, Dankook University , Cheonan 330-714, South Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine; D003907:Dexamethasone
keywords: cell adhesion; drug loading; electrodeposition; implants; mesoporous nanotubes; nanostructured coating