title: New and Improved Techniques for the Study of Pathogenic Fungi.
abstract: Fungal pathogens pose serious threats to human, plant, and ecosystem health. Improved diagnostics and antifungal strategies are therefore urgently required. Here, we review recent developments in online bioinformatic tools and associated interactive data archives, which enable sophisticated comparative genomics and functional analysis of fungal pathogens in silico. Additionally, we highlight cutting-edge experimental techniques, including conditional expression systems, recyclable markers, RNA interference, genome editing, compound screens, infection models, and robotic automation, which are promising to revolutionize the study of both human and plant pathogenic fungi. These novel techniques will allow vital knowledge gaps to be addressed with regard to the evolution of virulence, host-pathogen interactions and antifungal drug therapies in both the clinic and agriculture. This, in turn, will enable delivery of improved diagnosis and durable disease-control strategies.
authors.affiliation: Institut für Biotechnologie, Technische Universität Berlin, Gustav-Meyer Allee 22, Berlin, Germany. Electronic address: t.cairns@campus.tu-berlin.de.
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Cairns
authors.affiliation: Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Studholme
authors.affiliation: Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Talbot
authors.affiliation: Biosciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UK.
authors.forename: Ken
authors.initials: K
authors.lastname: Haynes
mesh_terms: D019295:Computational Biology / Q000379:methods; D005658:Fungi / Q000235:genetics* / Q000472:pathogenicity*; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D023281:Genomics / Q000379:methods; D054884:Host-Pathogen Interactions; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D014774:Virulence
keywords: antifungal; comparative genomics; functional genomics; fungal pathogen; genome editing