title: Structural characterization and effect on anti-angiogenic activity of a fucoidan from Sargassum fusiforme.
abstract: A fucoidan FP08S2 was isolated from the boiling-water extract of Sargassum fusiforme, purified by CaCl2 precipitation and chromatography on DEAE-cellulose and Sephacryl S-300. FP08S2 contained fucose, xylose, galactose, mannose, glucuronic acid, and 20.8% sulfate. The sulfate groups were attached to diverse positions of fucose, xylose, mannose, and galactose residues. The backbone of FP08S2 consisted of alternate 1,2-linked α-D-Manp and 1,4-linked β-D-GlcpA. Sugar composition analysis and ESI-MS revealed that the oligosaccharides from branches contained fucose, xylose, galactose, glucuronic acid and sulfate. FP08S2 could significantly inhibit tube formation and migration of human microvascular endothelial cells (HMEC-1) dose-dependently. These results suggested that the fucoidan FP08S2 from brown seaweeds S. fusiforme could be a potent anti-angiogenic agent.
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Qifei
authors.initials: Q
authors.lastname: Cong
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Huanjun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Liao
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China. Electronic address: dongqun@simm.ac.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Glycochemistry and Glycobiology Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang, Pudong, Shanghai 201203, China. Electronic address: dingkan@simm.ac.cn.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D044647:Sargassum / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D020533:Angiogenesis Inhibitors; D009005:Monosaccharides; D011134:Polysaccharides; C007789:fucoidan
keywords: Anti-angiogenic activity; Fucoidan; Sargassum fusiforme; Structure