title: Lentivirus-Mediated knockdown of tectonic family member 1 inhibits medulloblastoma cell proliferation.
abstract: Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in Hedgehog (Hh) signaling during mouse embryonic development and was imperative for Hh-mediated patterning of the ventral neural tube. However, the expression and regulation of tectonics in human tumor is still not clear. In this study, shRNA-expressing lentivirus was constructed to knockdown TCTN1 in medulloblastoma cell line Daoy. The results showed that knockdown of TCTN1 inhibited cell proliferation and colony formation in Daoy cell line, also caused cell cycle arrest at the G2/M boundary. Taken all together, our data suggest that TCTN1 might play an important role in the progression of medulloblastoma.
authors.affiliation: Neurosurgery, Fuzhou General Hospital of Second Military Medical University Fuzhou 350025.
authors.forename: Junjie
authors.initials: J
authors.lastname: Jing
authors.affiliation: Department of Pediatrics, Fuzhou General Hospital of Second Military Medical University Fuzhou 350025.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Pediatric Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University, School of Medicine Shanghai 200092.
authors.forename: Qinchuan
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Pediatric Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University, School of Medicine Shanghai 200092.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Neurosurgery, Fuzhou General Hospital of Second Military Medical University Fuzhou 350025.
authors.forename: Qingshuang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Neurosurgery, Fuzhou General Hospital of Second Military Medical University Fuzhou 350025.
authors.forename: Shousen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Pediatric Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University, School of Medicine Shanghai 200092.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
keywords: Daoy; RNA interference; medulloblastoma; proliferation; tectonic family member 1