title: Personalizing therapy for multidrug resistant TB: the potential of Rapid Whole Genome Sequencing.
abstract: Multidrug resistant tuberculosis is an increasing problem globally. The current gold standard in drug sensitivity testing is slow and cumbersome. To tackle drug resistance effectively, a more rapid method of testing is required. Current molecular tests are fast, but only offer information on a limited number of genetic loci. Whole genome sequencing presents an attractive alternative that can provide comprehensive, clinically relevant information on all described loci. Although the standard approach to whole genome sequencing of Mycobacterium tuberculosis is slow due to the requirement of culture, this article will describe recent advances that mean it has the potential to provide results within days.
authors.affiliation: a Division of Infection and Immunity , UCL , London , UK.
authors.forename: Josephine M
authors.initials: JM
authors.lastname: Bryant
authors.affiliation: b Centre for Respiratory Medicine, Royal Free London NHS Foundation Trust , University College London , London , UK.
authors.forename: Marc
authors.initials: M
authors.lastname: Lipman
authors.affiliation: a Division of Infection and Immunity , UCL , London , UK.
authors.forename: Judith
authors.initials: J
authors.lastname: Breuer
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques / Q000379:methods*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D056426:Genetic Loci; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008826:Microbial Sensitivity Tests; D058990:Molecular Targeted Therapy; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D057285:Precision Medicine; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents
keywords: DST; WGS; genomics; sequencing; tuberculosis