title: IL-6/STAT3/SOCS3 signaling pathway playing a regulatory role in ulcerative colitis carcinogenesis.
abstract: OBJECTIVE
  Large-scale clinical studies have shown that ulcerative colities were related with colorectal cancer. In this study, animal model was established by AOM/DSS method to explore the activation of IL-6-STAT3-SOCS3 signaling pathway and the expression of pathway-related proteins in ulcerative colitis carcinogenesis, in order to lay a foundation for exploring the regulation mechanism of IL-6/STAT3/SOCS3 signaling pathway in ulcerative colitis carcinogenesis.
  
  
  METHODS
  AOM/DSS modeling method was used to establish animal models of ulcerative colitis carcinogenesis; colonic mucosa specimens were collected at different time points for pathological examination. Immunohistochemical method and western blot were used to detect the expression of IL6, STAT3 and SOCS3 protein in the control group, UC model + empty vector group and UC model + STAT3 knockout group.
  
  
  RESULTS
  In UC model + empty vector group, IL6 and STAT3 expression was increased as lesion degree increased (P < 0.05). The expression of SOCS3 was weakened and the degree of activation decreased (P < 0.05). IL6 expression increased in UC model + STAT3 knockout group (P < 0.05) while the expression of SOCS3 decreased; compared with the UC model + empty vector group, there was a significant difference (P < 0.05).
  
  
  CONCLUSIONS
  The expression and activation of IL6 and STAT3 expression were enhanced in ulcerative colitis carcinogenesis, and their expression increased with the lesion degree increased, reflecting the disease progression to a certain extent. The expression and activation of SOCS3 expression decreased. STAT3 had a certain effect on the expression of SOCS3, playing a certain regulatory role in ulcerative colitis carcinogenesis.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Ma
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Dong-Yue
authors.initials: DY
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Sun
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Xun-Hai
authors.initials: XH
authors.lastname: Yin
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Guo-Yin
authors.initials: GY
authors.lastname: Sang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Yin-Hua
authors.initials: YH
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang Province, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
keywords: IL-6; SOCS3; STAT3; Ulcerative colitis; signaling pathways