title: HIV-1 Virion Production from Single Inducible Proviruses following T-Cell Activation Ex Vivo.
abstract: Quantifying induced virion production from single proviruses is important for assessing the effects of HIV-1 latency reversal agents. Limiting dilution ex vivo cultures of resting CD4(+) T cells from 14 HIV-positive volunteers revealed that virion production after T-cell activation from individual proviruses varies by 10,000-fold to 100,000-fold. High-producing proviruses were associated with increases in cell-associated HIV-1 DNA levels, suggesting that reactivated proviruses proliferate. Single-cell analyses are needed to investigate differences in proviral expansion and virus production following latency reversal.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA JOB74@pitt.edu arc85@pitt.edu.
authors.forename: John K
authors.initials: JK
authors.lastname: Bui
authors.affiliation: Division of Infectious Diseases, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Mellors
authors.affiliation: Division of Infectious Diseases, Department of Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA JOB74@pitt.edu arc85@pitt.edu.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Cillo
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D015658:HIV Infections / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation; D011533:Proviruses / Q000502:physiology*; D014771:Virion / Q000302:isolation & purification*; D014775:Virus Activation; D014779:Virus Replication
chemical_list: D004279:DNA, Viral