title: A triphenylamine-based colorimetric and fluorescent probe with  donor–bridge–acceptor structure for detection of G-quadruplex DNA.
abstract: In this Letter, three triphenylamine-based dyes (TPA-1, TPA-2a and TPA-2b) with donor–bridge–acceptor (D–p–A) structure were designed and synthesized for the purpose of G-quadruplexes recognition. In aqueous conditions, the interactions of the dyes with G-quadruplexes were studied with the aim to establish the influence of the geometry of the dyes on their binding and probing properties. Results indicate that TPA-2b displays significant selective colorimetric and fluorescent changes upon binding of G-quadruplex DNA. More importantly, its distinct color change enables visual detection and differentiation of G-quadruplexes from single and duplex DNA structures. CD titration date reveals that TPA-2b could induce and stabilize the formation of G-quadruplex structure. All these remarkable properties of TPA-2b suggest that it should have promising application in the field of G-quadruplexes research.
authors.affiliation: School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China
authors.forename: Ming-Qi
authors.initials: MQ
authors.lastname: Wang
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Zhu
authors.forename: Zhuan-Zhuan
authors.initials: ZZ
authors.lastname: Song
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.forename: Yong-Zhao
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D002942:Circular Dichroism; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes
chemical_list: D005456:Fluorescent Dyes; D004247:DNA