title: Contracting C2C12 myotubes release CCL2 in an NF-κB-dependent manner to induce monocyte chemoattraction.
abstract: Muscle inflammation following exercise is characterized by expression of inflammatory cytokines and chemokines. Exercise also increases muscle macrophages derived from circulating monocytes. However, it is unknown whether muscle cells themselves attract circulating monocytes, or what is the underlying mechanism. We used an in vitro system of electrical stimulation (ES) causing C2C12 myotube contraction to explore whether monocyte chemoattraction ensues and investigated the mediating chemoattractants. Conditioned medium from ES-contracted myotubes caused robust chemoattraction of THP-1 monocytes across Boyden chambers. Following ES, expression of several known monocyte chemokines [C-C motif ligand 2 (CCL2) and C-X-C motif ligand (CXCL)1, -2, and -5] was elevated, but of these, only recombinant CCL2 effectively reproduced monocyte migration. Electrically stimulated myotubes secreted CCL2, and neutralization of CCL2 in conditioned medium or antagonizing the CCL2 receptor (CCR2) in THP-1 monocytes inhibited ES-induced monocyte migration. N-benzyl-p-toluene sulfonamide (BTS), a myosin II-ATPase inhibitor, prevented ES-induced myotube contraction but not CCL2 gene expression and secretion. The membrane-permeant calcium chelator BAPTA-AM reduced ES-induced CCL2 secretion. Hence, electrical depolarization, rather than mechanical contraction, drives the rise in CCL2, with partial calcium input. ES activated the NF-κB pathway; NF-κB inhibitors reduced ES-induced CCL2 gene expression and secretion and repressed ES-induced THP-1 chemoattraction. Thus, electrically stimulated myotubes chemoattract monocytes through NF-κB-regulated CCL2 secretion.
authors.affiliation: Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Shouta
authors.initials: S
authors.lastname: Miyatake
authors.affiliation: Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Bilan
authors.affiliation: Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: Nicolas J
authors.initials: NJ
authors.lastname: Pillon
authors.affiliation: Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario, Canada amira@sickkids.ca.
authors.forename: Amira
authors.initials: A
authors.lastname: Klip
mesh_terms: D000818:Animals; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism*; D002633:Chemotaxis / Q000502:physiology*; D004558:Electric Stimulation; D006801:Humans; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D009119:Muscle Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2; D016328:NF-kappa B
keywords: CCL2/MCP-1; contraction; monocyte migration; myokine; skeletal muscle