title: Enhanced production and application of acidothermophilic Streptomyces cellulase.
abstract: An efficient cellulolytic and acidothermophilic actinobacterium was isolated from soil, adhered to decomposing tree bark and was identified as Streptomyces DSK59. Screening of synthetic media and the media components identified that, a medium based on starch casein minerals containing carboxy methyl cellulose (CMC) and beef extract (BE) could support enhanced cellulase production by the organism. CMC, BE, NaCl, temperature and pH were accounted as significant for cellulase production and these were optimized using a response surface central composite design (CCD). Optimization of cellulase production resulted in an enhancement of endoglucanase activity to 27IUml(-1). Acidothermophillic Streptomyces cellulase was found to be efficient for hydrolysis of pretreated sorghum stover and liberated 0.413gg(-1) of total reducing sugars which was higher than previously reported sugar yields obtained using fungal enzymes.
authors.affiliation: A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Kalaburagi 585106, India.
authors.forename: Saikumar R
authors.initials: SR
authors.lastname: Budihal
authors.affiliation: A-DBT Research Laboratory, Department of Microbiology, Gulbarga University, Kalaburagi 585106, India. Electronic address: dayanandagsar@gmail.com.
authors.forename: Dayanand
authors.initials: D
authors.lastname: Agsar
authors.affiliation: Department of Biotechnology, Vishweshwariah College of Applied Sciences, Kalaburagi 585103, India.
authors.forename: Sarvamangala R
authors.initials: SR
authors.lastname: Patil
mesh_terms: D000704:Analysis of Variance; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D045868:Sorghum / Q000187:drug effects / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D013696:Temperature
chemical_list: D002241:Carbohydrates; D002244:Carbon; D002480:Cellulase
keywords: Acidothermophilic Streptomyces; Biomass hydrolysis; Cellulase; Enzyme cocktail