title: Modulation of hepatocarcinogenesis in N-methyl-N-nitrosourea treated Balb/c mice by mushroom extracts.
abstract: The hepatoprotective potential of edible mushrooms from Mauritius, namely Pleurotus sajor-caju and Agaricus bisporus was evaluated using an N-methyl-N-nitrosourea (MNU)-induced hepatocarcinogenesis Balb/c mice model. Mushroom extracts restored normal weight in MNU treated mice over a 3 month supplementation period. Blood parameter analyses indicated a clear modulation of hemoglobin concentration, leukocyte, platelet, lymphocyte, neutrophil, monocyte and eosinophil counts in MNU-induced mice (p < 0.05). Mushroom extract supplementation effectively reduced oxidative damage in MNU-primed mice, which was marked by a significant decrease in the extent of lipid peroxidation (p < 0.05) and a concomitant increase in the enzymatic antioxidant levels, primarily catalase, superoxide dismutase, glutathione reductase and peroxidase, and FRAP values (p < 0.05). DNA protective effects of the extracts were confirmed by Raman spectroscopy, where, the MNU-DNA interaction, as evidenced by an intense peak at 1254 cm(-1), was normalized. The findings demonstrate hepatoprotective, immunomodulatory and anti-carcinogenic effects and suggest the use of mushrooms as potential dietary prophylactics in cancer chemoprevention.
authors.affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, Republic of Mauritius. v.neergheen@uom.ac.mu and ANDI Centre of Excellence for Biomedical and Biomaterials Research, CBBR, Building, MSIRI, University of Mauritius, Réduit, Republic of Mauritius. tbahorun@uom.ac.mu.
authors.forename: Srishti
authors.initials: S
authors.lastname: Ramsaha
authors.affiliation: Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, Republic of Mauritius. v.neergheen@uom.ac.mu and ANDI Centre of Excellence for Biomedical and Biomaterials Research, CBBR, Building, MSIRI, University of Mauritius, Réduit, Republic of Mauritius. tbahorun@uom.ac.mu.
authors.forename: Vidushi S
authors.initials: VS
authors.lastname: Neergheen-Bhujun
authors.affiliation: Institute of Biosciences and Biotechnology, Chhatrapati Shahuji Maharaj University, Kanpur, Uttar Pradesh, India.
authors.forename: Shalini
authors.initials: S
authors.lastname: Verma
authors.affiliation: Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Institute of Biosciences and Biotechnology, Chhatrapati Shahuji Maharaj University, Kanpur, Uttar Pradesh, India.
authors.forename: Rahul Kumar
authors.initials: RK
authors.lastname: Bharty
authors.affiliation: Institute of Biosciences and Biotechnology, Chhatrapati Shahuji Maharaj University, Kanpur, Uttar Pradesh, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Chaudhary
authors.affiliation: Department of Physics, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Poornima
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Department of Physics, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Mushroom Unit, Food and Agricultural Research and Extension Institute, La Brasserie, Republic of Mauritius.
authors.forename: Priya
authors.initials: P
authors.lastname: Huzar Futty Beejan
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University, Seoul, South Korea.
authors.forename: Kang
authors.initials: K
authors.lastname: Kyung-Sun
authors.affiliation: ANDI Centre of Excellence for Biomedical and Biomaterials Research, CBBR, Building, MSIRI, University of Mauritius, Réduit, Republic of Mauritius. tbahorun@uom.ac.mu.
authors.forename: Theeshan
authors.initials: T
authors.lastname: Bahorun
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D063646:Carcinogenesis / Q000139:chemically induced*; D005260:Female; D008113:Liver Neoplasms / Q000139:chemically induced*; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation
chemical_list: D000970:Antineoplastic Agents; D008770:Methylnitrosourea