title: Effects of Atorvastatin on Oxidative Stress Biomarkers and Mitochondrial Morphofunctionality in Hyperfibrinogenemia-Induced Atherogenesis.
abstract: Relationship between hyperfibrinogenemia (HF), oxidative stress, and atherogenesis was established. Effect of atorvastatin (Ator) was assessed. Wistar male (6 months) rats were studied: Ctr, control, without HF induction; Ctr-Ator, without HF treated with atorvastatin; AI, atherogenesis induced, and AI-Ator, atherogenesis induced and treated with atorvastatin. Atherogenesis was induced by daily adrenaline injection (0.1 mL/day/rat) for 90 days; treatment started 15 days after induction. Fibrinogen (mg/dL) and nitric oxide (NO) were measured in plasma (mM) and superoxide dismutase (SOD) (U/mL) in red cell lysate by spectrophotometry. Slices of aorta were analyzed by electron microscopy (EM). ANOVA and chi-square test were used; P < 0.05 was established. There were no significant differences between Ctr and Ctr-Atorv in fibrinogen, NO, and SOD values. Comparing Ctr with AI an increase of fibrinogen is observed (P < 0.001), but it decreased after administration of atorvastatin in AI-Ator (P < 0.001). NO diminished in AI relative to Ctr and increased in AI-Ator (P < 0.001). SOD showed an increase in AI and AI-Ator compared to Ctr (P < 0.001). EM revealed expansion of intermembrane space and disorganization of crests in AI. In AI-Ator mitochondrial areas and diameters were similar to control. Atorvastatin normalizes HF, stabilizes NO, increases SOD, and produces a partial regression of mitochondrial lesions.
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina.
authors.forename: María de la Paz
authors.initials: Mde L
authors.lastname: Scribano
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina ; Instituto de Investigación en Ciencias de la Salud Humana (IICSHUM), Universidad Nacional de La Rioja, La Rioja, Argentina.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Baez
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina.
authors.forename: Becerra
authors.initials: B
authors.lastname: Florencia
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina ; Becaria Secyt, Universidad Nacional de Córdoba, Córdoba, Argentina.
authors.forename: Mariana Denise
authors.initials: MD
authors.lastname: Tarán
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina.
authors.forename: Signorini
authors.initials: S
authors.lastname: Franco
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina ; Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de La Rioja, F5300 La Rioja, Argentina.
authors.forename: Ariel G
authors.initials: AG
authors.lastname: Balceda
authors.affiliation: Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, X5000 Córdoba, Argentina ; Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de La Rioja, F5300 La Rioja, Argentina.
authors.forename: Mónica
authors.initials: M
authors.lastname: Moya