title: Positive/negative surface charge of chitosan based nanogels and its potential influence on oral insulin delivery.
abstract: To develop insulin delivery system for the treatment of diabetes, two insulin-loaded nanogels with opposite zeta potential (-15.94 ± 0.449 mV for insulin:CMCS/CS-NGs(-) and +17.15 ± 0.492 mV for insulin:CMCS/CS-NGs(+)) were obtained. Ex vivo results showed that the nanogels with opposite surface charge exhibited different adhesion and permeation in specific intestinal segments. There was no significant differences in adhesion and permeation in rat duodenum, but in rat jejunum, insulin:CMCS/CS-NGs(-) exhibited enhanced adhesion and permeation, which were about 3 folds (adhesion) and 1.7 folds (permeation) higher than insulin:CMCS/CS-NGs(+). These results demonstrated that the surface charge property of nanogels determined the absorption sites of CMCS/CS-NGs in small intestine. In vivo study, the blood glucose level in insulin:CMCS/CS-NGs(-) group had 3 mmol/L lower than insulin:CMCS/CS-NGs(+) group during 1h to 11h after the oral administration, which demonstrated that negative insulin:CMCS/CS-NGs had a better management of blood glucose than positive ones.
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China.
authors.forename: Mengxue
authors.initials: M
authors.lastname: Xu
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Kong
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China. Electronic address: fengchao@ouc.edu.cn.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.affiliation: College of Marine Life Science, Ocean University of China, 5# Yushan Road, Qingdao, 266003, Shandong, PR China. Electronic address: xgchen@ouc.edu.cn.
authors.forename: Xiguang
authors.initials: X
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018938:Caco-2 Cells; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D006801:Humans; D007328:Insulin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D051381:Rats; D055672:Static Electricity
chemical_list: D004337:Drug Carriers; D005782:Gels; D007328:Insulin; D048271:Chitosan
keywords: CMCS/CS-nanogels; Insulin; Oral delivery; Surface charge