title: Impacts of produced water origin on bacterial community structures of activated sludge.
abstract: The purpose of this study was to reveal how activated sludge communities respond to influent quality and indigenous communities by treating two produced waters from different origins in a batch reactor in succession. The community shift and compositions were investigated using Polymerase Chain Reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and further 16S ribosomal DNA (rDNA) clone library analysis. The abundance of targeted genes for polycyclic aromatic hydrocarbon (PAH) degradation, nahAc/phnAc and C12O/C23O, was tracked to define the metabolic ability of the in situ microbial community by Most Probable Number (MPN) PCR. The biosystem performed almost the same for treatment of both produced waters in terms of removals of chemical oxygen demand (COD) and PAHs. Sludge communities were closely associated with the respective influent bacterial communities (similarity>60%), while one sludge clone library was dominated by the Betaproteobacteria (38%) and Bacteriodetes (30%) and the other was dominated by Gammaproteobacteria (52%). This suggested that different influent and water quality have an effect on sludge community compositions. In addition, the existence of catabolic genes in sludge was consistent with the potential for degradation of PAHs in the treatment of both produced waters.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Key Laboratory for Microorganisms and Functional Molecules, College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China. Electronic address: zywang@htu.edu.cn.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Genetics Department, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt.
authors.forename: Abd El-Latif
authors.initials: Ael-L
authors.lastname: Hesham
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: gyx@rcees.ac.cn.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: zhangyu@rcees.ac.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012722:Sewage / Q000382:microbiology*; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018508:Water Purification; D060753:Water Quality
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012722:Sewage; D014874:Water Pollutants, Chemical; D014867:Water
keywords: Functional gene; Indigenous community; Polycyclic aromatic hydrocarbons; Wastewater treatment