title: Nuclear staining of fgfr-2/stat-5 and runx-2 in mucinous breast cancer.
abstract: Mucinous carcinoma (MBC) is a rare subtype of breast cancer characterized by the production of variable amounts of mucin, with a prognosis better than that of non-mucinous carcinomas (NMBC). The aim of this project was to evaluate the expression of STAT-5, RUNX-2, and FGFR-2 in a cohort of MBC and compare it with that of NMBC using standard immunohistochemistry. STAT-5 and RUNX-2 are two transcription factors with cytoplasmic and/or nuclear localization that have been related to FGFR-2, a tyrosine kinase growth factor receptor that can interact with STAT-5 and with PR in the nuclei of breast cancer cells. Membranous, cytoplasmic, and nuclear staining were evaluated and expressed as the percentage of stained cells (0-100%) multiplied by the staining intensity (0-3), thus obtaining an index ranging from 0 to 300. Nuclear and/or cytoplasmic immunoreactivity of the three proteins were detected in a high number of NMBC. Nuclear FGFR-2 staining correlated with nuclear STAT-5 (p<0.05) and nuclear RUNX-2 (p<0.01) in both tumor types; however MBC had a significant higher expression of nuclear FGFR-2 (p<0.01) and RUNX-2 (p<0.05) than that of NMBC, and displayed positive immunoreactivity of the 3 proteins in 70.8% of the cases. These results suggest that these proteins may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect.
authors.affiliation: Laboratory of Hormonal Carcinogenesis, Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: May
authors.affiliation: Breast Pathology Consultant in Buenos Aires, Argentina.
authors.forename: Julián
authors.initials: J
authors.lastname: Mosto
authors.affiliation: Magdalena V de Martinez Hospital, General Pacheco, Tigre, Argentina.
authors.forename: Paula Martinez
authors.initials: PM
authors.lastname: Vazquez
authors.affiliation: Magdalena V de Martinez Hospital, General Pacheco, Tigre, Argentina.
authors.forename: Pedro
authors.initials: P
authors.lastname: Gonzalez
authors.affiliation: Laboratory of Hormonal Carcinogenesis, Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina.
authors.forename: Paola
authors.initials: P
authors.lastname: Rojas
authors.affiliation: Magdalena V de Martinez Hospital, General Pacheco, Tigre, Argentina.
authors.forename: Hugo
authors.initials: H
authors.lastname: Gass
authors.affiliation: Laboratory of Hormonal Carcinogenesis, Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lanari
authors.affiliation: Department of Pathology and Moores Cancer Center, University of California San Diego, USA. Electronic address: amolinolo@ucsd.edu.
authors.forename: Alfredo A
authors.initials: AA
authors.lastname: Molinolo
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000175:diagnosis* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D011379:Prognosis; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000378:metabolism*; D050799:STAT5 Transcription Factor / Q000378:metabolism*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D050799:STAT5 Transcription Factor; C496353:FGFR2 protein, human; D051497:Receptor, Fibroblast Growth Factor, Type 2
keywords: Breast cancer; FGFR-2; Mucinous breast carcinoma; RUNX-2; STAT-5