title: Temperature Tolerance and Inactivation of Chikungunya Virus.
abstract: In late 2013, chikungunya virus (CHIKV) was introduced to the New World and large outbreaks occurred in the Caribbean islands causing over a million suspected and over 20,000 laboratory-confirmed cases. Serological analysis is an essential component for the diagnosis of CHIKV infection together with virus isolation and detection of viral nucleic acid. Demonstrating virus neutralizing by serum antibodies in a plaque reduction neutralization test (PRNT) is the gold standard of all serological diagnostic assays. Prior to the testing, heat inactivation of serum at 56°C for 30 min is required for the inactivation of complement activity and adventitious viruses. The presence of adventitious contaminating viruses may interfere with the results by leading to a higher number of plaques on the monolayers and subsequent false-negative results. This procedure is widely accepted for the inactivation of flaviviruses and alphaviruses. In this study, the thermostability of CHIKV was evaluated. Heat inactivation at 56°C for 30 min was demonstrated to be insufficient for the complete removal of infectious CHIKV virions present in the samples. This thermotolerance of CHIKV could compromise the accuracy of serum tests, and therefore longer treatment for greater than 120 min is recommended.
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.|2 Biosecurity Research Institute, Kansas State University , Manhattan, Kansas.
authors.forename: Yan-Jang S
authors.initials: YJ
authors.lastname: Huang
authors.affiliation: 3 Department of Statistics, College of Arts and Sciences, Kansas State University , Manhattan, Kansas.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Hsu
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.|2 Biosecurity Research Institute, Kansas State University , Manhattan, Kansas.
authors.forename: Stephen
authors.initials: S
authors.lastname: Higgs
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.|2 Biosecurity Research Institute, Kansas State University , Manhattan, Kansas.
authors.forename: Dana L
authors.initials: DL
authors.lastname: Vanlandingham
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D002646:Chikungunya virus / Q000276:immunology / Q000502:physiology*; D006358:Hot Temperature; D009500:Neutralization Tests; D014771:Virion / Q000276:immunology / Q000502:physiology; D038301:Virus Inactivation
chemical_list: D000914:Antibodies, Viral
keywords: Chikungunya virus; Heat inactivation; Thermostability