title: Biomineralization of calcium phosphate crystals on chitin nanofiber hydrogel for bone regeneration material.
abstract: We previously reported a chitin nanofiber hydrogel from squid pen β-chitin by a simple NaOH treatment. In the present study, a calcium phosphate/chitin nanofiber hydrogel was prepared for bone tissue engineering. Calcium phosphate was mineralized on the hydrogel by incubation in a solution of diammonium hydrogen phosphate solution followed by calcium nitrate tetrahydrate. X-ray diffractometry and Fourier transform infrared spectroscopy showed the formation of calcium phosphate crystals. The morphology of the calcium phosphate crystals changed depending on the calcification time. After mineralization, the mechanical properties of the hydrogel improved due to the reinforcement effect of calcium phosphate crystal. In an animal experiment, calcium phosphate/chitin nanofiber hydrogel accelerated mineralization in subcutaneous tissues. Morphological osteoblasts were observed.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Kawata
authors.affiliation: Department of Veterinary Clinical Medicine, Tottori University, Tottori 680-8553, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Izawa
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Morimoto
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Saimoto
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan. Electronic address: sifuku@chem.tottori-u.ac.jp.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ifuku
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D002130:Calcium Phosphates / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D002130:Calcium Phosphates; D020100:Hydrogels; D002686:Chitin; C020243:calcium phosphate
keywords: Bone regeneration; Calcium phosphate; Chitin; Hydrogel; Nanofibers