title: An audit of clinical laboratory data of 25 [OH]D at Aga Khan University as reflecting vitamin D deficiency in Pakistan.
abstract: OBJECTIVE
  To estimate the burden of Vitamin D deficiency in people from different geographical areas of Pakistan.
  
  
  METHODS
  The retrospective study was conducted at the Aga Khan University Hospital, Karachi, and comprised specimens of subjects tested for 25-hydroxy D at the clinical laboratory between September 2010 and September 2011. Serum samples received from the phlebotomy centres all over Pakistan and the main laboratory in Karachi were included. SPSS 19 was used for statistical analysis.
  
  
  RESULTS
  Of the 60937 specimens in the study, 18721(30.7%) related to men. The overall mean age was 40.5±19.7 years, and median 25-hydroxy D level was 13.5ng/ml (interquartile range: 25.1-7.4 ng/ml).Overall, mean log 25-hydroxy D was 1.14±0.39ng/ml (median: 13.5ng/ml; interquartile range: 25.1-7.4 ng/ml). Out of the total, 40279(66.1%) subjects were vitamin D-deficient.
  
  
  CONCLUSIONS
  Vitamin D deficiency was common among the subjects.
authors.affiliation: Medical Student, Aga Khan University, Karachi.
authors.forename: Sidra
authors.initials: S
authors.lastname: Hassan
authors.affiliation: Medical Student, Aga Khan University, Karachi.
authors.forename: Syeda Maria
authors.initials: SM
authors.lastname: Muzammil
authors.affiliation: Department of Pathology and Microbiology, Aga Khan University, Karachi.
authors.forename: Lena
authors.initials: L
authors.lastname: Jafri
authors.affiliation: Department of Pathology and Microbiology, Aga Khan University, Karachi.
authors.forename: Aysha Habib
authors.initials: AH
authors.lastname: Khan
mesh_terms: D000328:Adult; D002112:Calcifediol / Q000097:blood; D054869:Clinical Audit; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan / Q000453:epidemiology; D012189:Retrospective Studies; D014808:Vitamin D Deficiency / Q000453:epidemiology*; D055815:Young Adult
chemical_list: D002112:Calcifediol
keywords: Vitamin D, Deficiency, Prevalence.