title: Does Leishmaniasis disease alter the parenchyma and protein expression in salivary glands?
abstract: Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB/c male mice, around six- to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15 ml of NaCl, while those in the experimental group were inoculated with 5 × 10(6) amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-β-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the β-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil airtoncondejr@uol.com.br.
authors.forename: Aírton M C
authors.initials: AM
authors.lastname: Júnior
authors.affiliation: Department of Biochemistry and Pharmacology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: de Amorim Carvalho
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Weslany
authors.initials: W
authors.lastname: de Oliveira Dantas
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Luana C L
authors.initials: LC
authors.lastname: Gomes
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Andrezza B S
authors.initials: AB
authors.lastname: da Silva
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Maria M A
authors.initials: MM
authors.lastname: de Sousa Cavalcante
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Ingrid M
authors.initials: IM
authors.lastname: de Oliveira
authors.affiliation: Department of Patology and Odontological Clinic, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Marina
authors.initials: M
authors.lastname: de Deus Moura de Lima
authors.affiliation: Department of Clinical and Veterinary Surgery, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Márcia Dos Santos
authors.initials: Mdos S
authors.lastname: Rizzo
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Carla Maria
authors.initials: CM
authors.lastname: de Carvalho Leite
authors.affiliation: Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Selma Maria Dos Santos
authors.initials: SM
authors.lastname: Moura
authors.affiliation: Department of Patology and Odontological Clinic, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Lúcia de Fátima Almeida
authors.initials: Lde F
authors.lastname: de Deus Moura
authors.affiliation: Maternal Child Departament, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
authors.forename: Benedito B
authors.initials: BB
authors.lastname: da Silva
mesh_terms: D000818:Animals; D053143:Caspase 2 / Q000032:analysis; D004195:Disease Models, Animal; D006651:Histocytochemistry; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D018314:Leishmania infantum / Q000254:growth & development; D007896:Leishmaniasis / Q000469:parasitology / Q000473:pathology*; D008297:Male; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D010306:Parotid Gland / Q000473:pathology*; D013361:Sublingual Gland / Q000473:pathology*; D013363:Submandibular Gland / Q000473:pathology*; D051176:beta Catenin / Q000032:analysis
chemical_list: C495267:CTNNB1 protein, mouse; D019394:Ki-67 Antigen; C498355:Mki67 protein, mouse; D051176:beta Catenin; D053143:Caspase 2
keywords: Leishmaniasis; antibodies; beta catenin; caspase; immunohistochemistry; salivary glands