title: Crystalline Bacterial Surface Layer (S-Layer) Opens Golden Opportunities for Nanobiotechnology in Textiles.
abstract: This study focuses on the successful recrystallization of bacterial S-layer arrays of the Lactobacillus acidophilus ATCC 4356 at textile surfaces to create a novel method and material. Optimum bacterial growth was obtained at approximately 45 °C, pH 5.0, and 14 h pi. The cells were resuspended in guanidine hydrochloride and the 43 kDa S-protein was dialyzed and purified. The optimum reassembly on the polypropylene fabric surface in terms of scanning electron microscopy (SEM), reflectance, and uniformity (spectrophotometry) was obtained at 30 °C, pH 5.0 for 30 minutes in the presence of 2 gr/l (liquor ratio; 1:40) of the S-protein. Overall, our data showed that the functional aspects and specialty applications of the fabric would be very attractive for the textile and related sciences, and result in advanced technical textiles.
authors.forename: Narges
authors.initials: N
authors.lastname: Asadi
authors.forename: Nima
authors.initials: N
authors.lastname: Chand
authors.forename: Mehdi
authors.initials: M
authors.lastname: Rassa
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods*; D003460:Crystallization; D057927:Hydrophobic and Hydrophilic Interactions; D007779:Lactobacillus acidophilus / Q000737:chemistry; D008422:Materials Testing; D008562:Membrane Glycoproteins / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D013784:Textiles
chemical_list: D001672:Biocompatible Materials; D008562:Membrane Glycoproteins; C055764:S-layer proteins