title: Cancer Cell Radiobiological Studies Using In-House-Developed α-Particle Irradiator.
abstract: An α-particle irradiator, enabling high-precision irradiation of cells for in vitro studies, has been constructed. The irradiation source was a (241)Am source, on which well inserts containing cancer cells growing in monolayer were placed. The total radioactivity, uniformity, and α-particle spectrum were determined by use of HPGe detector, Gafchromic dosimetry film, and PIPS detector measurements, respectively. Monte Carlo simulations were used for dosimetry. Three prostate cancer (LNCaP, DU145, PC3) and three pancreatic cancer (Capan-1, Panc-1, BxPC-3) cell lines were irradiated by α-particles to the absorbed doses 0, 0.5, 1, and 2 Gy. For reference, cells were irradiated using (137)Cs to the absorbed doses 0, 1, 2, 4, 6, 8, and 10 Gy. Radiation sensitivity was estimated using a tetrazolium salt-based colorimetric assay with absorbance measurements at 450 nm. The relative biological effectiveness for α-particles relative to γ-irradiation at 37% cell survival for the LNCaP, DU145, PC3, Capan-1, Panc-1, and BxPC-3 cells was 7.9 ± 1.7, 8.0 ± 0.8, 7.0 ± 1.1, 12.5 ± 1.6, 9.4 ± 0.9, and 6.2 ± 0.7, respectively. The results show the feasibility of constructing a desktop α-particle irradiator as well as indicate that both prostate and pancreatic cancers are good candidates for further studies of α-particle radioimmunotherapy.
authors.affiliation: 1 Department of Radiation Physics, Gothenburg University , Gothenburg, Sweden .
authors.forename: Jenny
authors.initials: J
authors.lastname: Nilsson
authors.affiliation: 2 Department of Surgery, Institution of Clinical Sciences, Lund University , Lund, Sweden .
authors.forename: Monika Posaric
authors.initials: MP
authors.lastname: Bauden
authors.affiliation: 3 Department of Medical Radiation Physics, Lund University , Malmö, Sweden .
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Nilsson
authors.affiliation: 4 Department of Medical Radiation Physics, Institution of Clinical Sciences, Lund University , Lund, Sweden .
authors.forename: Sven-Erik
authors.initials: SE
authors.lastname: Strand
authors.affiliation: 4 Department of Medical Radiation Physics, Institution of Clinical Sciences, Lund University , Lund, Sweden .
authors.forename: Jörgen
authors.initials: J
authors.lastname: Elgqvist
mesh_terms: D000512:Alpha Particles / Q000627:therapeutic use*; D000576:Americium / Q000737:chemistry; D002470:Cell Survival / Q000528:radiation effects*; D002588:Cesium Radioisotopes / Q000493:pharmacokinetics; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D008297:Male; D010190:Pancreatic Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D011836:Radiation Tolerance / Q000528:radiation effects*; D011853:Radiobiology; D012062:Relative Biological Effectiveness; D014407:Tumor Cells, Cultured
chemical_list: D002588:Cesium Radioisotopes; D000576:Americium
keywords: dosimetry; high-LET radiation; pancreatic cancer; prostate cancer; radioimmunotherapy