title: Complexation Hydrogels as Oral Delivery Vehicles of Therapeutic Antibodies: An in Vitro and ex Vivo Evaluation of Antibody Stability and Bioactivity.
abstract: Oral administration of monoclonal antibodies (mAbs) may enable the localized treatment of infections or other conditions in the gastrointestinal tract (GI) as well as systemic diseases. As with the development of oral protein biotherapeutics, one of the most challenging tasks in antibody therapies is the loss of biological activity due to physical and chemical instabilities. New families of complexation hydrogels with pH-responsive properties have demonstrated to be excellent transmucosal delivery vehicles. This contribution focuses on the design and evaluation of hydrogel carriers that will minimize the degradation and maximize the in vivo activity of anti-TNF-α, a mAb used for the treatment of inflammatory bowel disease (IBD) in the GI tract and systemically for the treatment of rheumatoid arthritis. P(MAA-g-EG) and P(MAA-co-NVP) hydrogels systems were optimized to achieve adequate swelling behavior, which translated into improved protein loading and release at neutral pH simulating the small intestine conditions. Additionally, these hydrogel systems preserve antibody bioactivity upon release resulting in the systemic circulation of an antibody capable of effectively performing its biological function. The compatibility if these hydrogels for mAb bioactivity preservation and release makes them candidates for use as oral delivery systems for therapeutic antibodies.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712-1062, United States.
authors.forename: Brenda R
authors.initials: BR
authors.lastname: Carrillo-Conde
authors.affiliation: Department of Biomedical Engineering, Drexel University, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Erik
authors.initials: E
authors.lastname: Brewer
authors.affiliation: College of Engineering, Rowan University, Glassboro, New Jersey 08028, United States.
authors.forename: Anthony
authors.initials: A
authors.lastname: Lowman
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712-1062, United States ; Department of Chemical Engineering, The University of Texas at Austin, Austin, Texas 78712-1062, United States ; Division of Pharmaceutics, The University of Texas at Austin, Austin, Texas 78712-1062, United States.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Peppas