title: Heterogeneity of the Mac-1 expression on peripheral blood neutrophils in patients with different types of epithelial ovarian cancer.
abstract: The expression level of Mac-1 on the surface of neutrophils is an important indicator of neutrophil activation. Under pathological conditions, Mac-1 is believed a key adhesion molecule that facilitates cancer progression and mediates the adhesion of tumour cells to the endothelium of blood vessels. Our previous findings indicated that circulating peripheral blood neutrophils in patients with advanced epithelial ovarian cancer (EOC) expressed enhanced levels of Mac-1, which was functionally associated with an increased adhesive function of neutrophils. The objective of the current study was to analyse whether the value of individual components of the differential white cell count, including the neutrophil and lymphocyte ratios, which are markers of blood neutrophil activation, might be associated with certain types of ovarian cancer. We showed the increase in Mac-1 expression along with a parallel decrease of L-selectin and PSGL-1 on peripheral blood neutrophils of patients with EOC of early and advanced FIGO stages, which indicates an activated state of neutrophils in comparison to neutrophils of individuals without cancer. Despite a significant difference between Mac-1 expression in patients with and without cancer, a dramatic increase in Mac-1 expression was observed in the blood of patients with undifferentiated carcinomas compared with patients with other histological types of EOC. Moreover, the expression level of Mac-1 correlated with the number of neutrophils in patients with serous, endometrioid and undifferentiated EOC. The results of an ROC analysis demonstrated that the patients with the undifferentiated type of EOC form a distinct group with regard to Mac-1 expression on blood neutrophils. The results suggested a diverse biological cadre of immune cells in patients with undifferentiated ovarian carcinomas compared with patients with other histological types of EOC.
authors.affiliation: Department of Experimental Immunology, Institute for Medical Biology, Polish Academy of Sciences, Lodowa Street 102, 93-232 Lodz, Poland. Electronic address: kbednarska@cbm.pan.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bednarska
authors.affiliation: Department of Experimental Immunology, Institute for Medical Biology, Polish Academy of Sciences, Lodowa Street 102, 93-232 Lodz, Poland. Electronic address: mklink@cbm.pan.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Klink
authors.affiliation: Department of Gynaecology and Gynaecologic Oncology, Polish Mothers'Memorial Hospital-Research Institute, Rzgowska Street 281/289, 93-338 Lodz, Poland. Electronic address: sek22@iczmp.edu.pl.
authors.forename: Jacek R
authors.initials: JR
authors.lastname: Wilczyński
authors.affiliation: Department of Operative Gynaecology and Gynaecologic Oncology, Polish Mothers' Memorial Hospital-Research Institute, Rzgowska Street 281/289, 93-338 Lodz, Poland. Electronic address: sek24@iczmp.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szyłło
authors.affiliation: Department of Operative, Endoscopic Gynaecology and Gynaecologic Oncology, Polish Mothers' Memorial Hospital - Research Institute, Rzgowska Street 281/289, 93-338 Lodz, Poland. Electronic address: czmpgin@polbox.com.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Malinowski
authors.affiliation: Department of Experimental Immunology, Institute for Medical Biology, Polish Academy of Sciences, Lodowa Street 102, 93-232 Lodz, Poland. Electronic address: zsulowska@cbm.pan.pl.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Sułowska
authors.affiliation: Department of Gynaecology and Gynaecologic Oncology, Polish Mothers'Memorial Hospital-Research Institute, Rzgowska Street 281/289, 93-338 Lodz, Poland. Electronic address: mrn@poczta.onet.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Nowak
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D039481:CD11b Antigen / Q000235:genetics / Q000276:immunology; D002277:Carcinoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D019041:L-Selectin / Q000235:genetics / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D009367:Neoplasm Staging; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012372:ROC Curve; D015398:Signal Transduction
chemical_list: D039481:CD11b Antigen; C509295:ITGAM protein, human; D016177:Macrophage-1 Antigen; D019041:L-Selectin
keywords: CD11b; Histological classification; Mac-1; Neutrophil granulocytes; Ovarian cancer