title: Dissection of the genetic architecture of rice resistance to the blast fungus Magnaporthe oryzae.
abstract: Resistance in rice cultivars to the rice blast fungus Magnaporthe oryzae is complex and is controlled by both major genes and quantitative trait loci (QTLs). We undertook a genome-wide association study (GWAS) using the rice diversity panel 1 (RDP1) that was genotyped using a high-density (700 000 single nucleotide polymorphisms) array and inoculated with five diverse M. oryzae isolates. We identified 97 loci associated with blast resistance (LABRs). Among them, 82 were new regions and 15 co-localized with known blast resistance loci. The top 72 LABRs explained up to 98% of the phenotypic variation. The candidate genes in the LABRs encode nucleotide-binding site leucine-rich repeat (NBS-LRR) resistance proteins, receptor-like protein kinases, transcription factors and defence-related proteins. Among them, LABR_64 was strongly associated with resistance to all five isolates. We analysed the function of candidate genes underlying LABR_64 using RNA interference (RNAi) technology and identified two new resistance alleles at the Pi5 locus. We demonstrate an efficient strategy for rapid allele discovery using the power of GWAS, coupled with RNAi technology, for the dissection of complex blast resistance in rice.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Houxiang
authors.initials: H
authors.lastname: Kang
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Peng
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China.
authors.forename: Shaohong
authors.initials: S
authors.lastname: Qu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Tianjin Crop Research Institute, Tianjin Academy of Agriculture Sciences, Tianjin, 300112, China.
authors.forename: Shuangyong
authors.initials: S
authors.lastname: Yan
authors.affiliation: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Agronomy, Hunan Agricultural University, Changsha, Hunan, 410128, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Wende
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yuese
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Plant Breeding & Genetics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Pavel
authors.initials: P
authors.lastname: Korniliev
authors.affiliation: International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila 1301, Philippines.
authors.forename: Hei
authors.initials: H
authors.lastname: Leung
authors.affiliation: Department of Plant Breeding & Genetics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Mezey
authors.affiliation: Department of Plant Breeding & Genetics, Cornell University, Ithaca, NY, 14853, USA.
authors.forename: Susan R
authors.initials: SR
authors.lastname: McCouch
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.|Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D060467:Disease Resistance / Q000235:genetics*; D056426:Genetic Loci; D018745:Genome, Plant; D055106:Genome-Wide Association Study; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza / Q000235:genetics* / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D034622:RNA Interference; D017422:Sequence Analysis, DNA
chemical_list: D010940:Plant Proteins
keywords: QTL; SNP; genome-wide association study (GWAS); host resistance; rice blast