title: Molecular characterization of Myf5 and comparative expression patterns of myogenic regulatory factors in Siniperca chuatsi.
abstract: Myogenic regulatory factors (MRFs) are muscle-specific basic helix-loop-helix (bHLH) transcription factor that plays an essential role in regulating skeletal muscle development and growth. To investigate molecular characterization of Myf5 and compare the expressional patterns of the four MRFs, we cloned the Myf5 cDNA sequence and analyzed the MRFs expressional patterns using quantitative real-time polymerase chain reaction in Chinese perch (Siniperca chuatsi). Sequence analysis indicated that Chinese perch Myf5 and other MRFs shared a highly conserved bHLH domain with those of other vertebrates. Sequence alignment and phylogenetic tree showed that Chinese perch MRFs had the highest identity with the MRFs of Epinephelus coioides. Spatio-temporal expressional patterns revealed that the MRFs were primarily expressed in muscle, especially in white muscle. During embryonic development period, Myf5, MyoD and MyoG mRNAs had a steep increase at neurula stage, and their highest expressional level was predominantly observed at hatching period. Whereas the highest expressional level of the MRF4 was observed at the muscular effect stage. The expressional patterns of post-embryonic development showed that the Myf5, MyoD and MyoG mRNAs were highest at 90 days post-hatching (dph). Furthermore, starvation and refeeding results showed that the transcription of the MRFs in the fast skeletal muscle of Chinese perch responded quickly to a single meal after 7 days of fasting. It indicated that the MRFs might contribute to muscle recovery after refeeding in Chinese perch.
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Li
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China. Electronic address: 2621372124@qq.com.
authors.forename: Wu-Ying
authors.initials: WY
authors.lastname: Chu
authors.affiliation: Department of Bioengineering and Environmental Science, Changsha University, Changsha, Hunan 410003, China. Electronic address: jzhang@ccsu.edu.cn.
authors.forename: Jian-She
authors.initials: JS
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005215:Fasting / Q000378:metabolism; D005399:Fishes / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D051787:Myogenic Regulatory Factor 5
keywords: Expression pattern; Myogenic regulatory factors; Siniperca chuatsi; Starvation/refeeding