title: Goldfish (Carassius auratus L.) as a model system to study the growth factors, receptors and transcription factors that govern myelopoiesis in fish.
abstract: The process of myeloid cell development (myelopoiesis) in fish has mainly been studied in three cyprinid species: zebrafish (Danio rerio), ginbuna carp (Carassius auratus langsdorfii) and goldfish (C. auratus, L.). Our studies on goldfish myelopoiesis have utilized in vitro generated primary kidney macrophage (PKM) cultures and isolated primary kidney neutrophils (PKNs) cultured overnight to study the process of macrophage (monopoiesis) and neutrophil (granulopoiesis) development and the key growth factors, receptors, and transcription factors that govern this process in vitro. The PKM culture system is unique in that all three subpopulations of macrophage development, namely progenitor cells, monocytes, and mature macrophages, are simultaneously present in culture unlike mammalian systems, allowing for the elucidation of the complex mixture of cytokines that regulate progressive and selective macrophage development from progenitor cells to fully functional mature macrophages in vitro. Furthermore, we have been able to extend our investigations to include the development of erythrocytes (erythropoiesis) and thrombocytes (thrombopoiesis) through studies focusing on the progenitor cell population isolated from the goldfish kidney. Herein, we review the in vitro goldfish model systems focusing on the characteristics of cell sub-populations, growth factors and their receptors, and transcription factors that regulate goldfish myelopoiesis.
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada. Electronic address: barb.katzenback@uwaterloo.ca.
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Katzenback
authors.affiliation: Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Katakura
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada.
authors.forename: Miodrag
authors.initials: M
authors.lastname: Belosevic
mesh_terms: D000818:Animals; D002450:Cell Communication; D029941:Fish Proteins / Q000502:physiology*; D006054:Goldfish; D006412:Hematopoietic Stem Cells / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology; D008954:Models, Biological; D038042:Myelopoiesis; D017978:Receptors, Growth Factor / Q000502:physiology; D015398:Signal Transduction; D014157:Transcription Factors / Q000502:physiology
chemical_list: D029941:Fish Proteins; D036341:Intercellular Signaling Peptides and Proteins; D017978:Receptors, Growth Factor; D014157:Transcription Factors
keywords: Erythrocytes; Goldfish; Growth factors; Macrophages; Myelopoiesis; Neutrophils; Progenitor cells; Thrombocytes; Transcription factors