title: Synergistic ameliorative effects of sesame oil and alpha-lipoic acid against subacute diazinon toxicity in rats: hematological, biochemical, and antioxidant studies.
abstract: Diazinon (DZN) is a common organophosphorus insecticide extensively used for agriculture and veterinary purposes. DZN toxicity is not limited to insects; it also induces harmful effects in mammals and birds. Our experiment evaluated the protective and antioxidant potential of sesame oil (SO) and (or) alpha-lipoic acid (ALA) against DZN toxicity in male Wistar albino rats. DZN-treated animals exhibited macrocytic hypochromic anemia and significant increases in serum biochemical parameters related to liver injury, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transferase (γGT), cholesterol, and triglycerides. They also had elevated levels of markers related to cardiac injury, such as lactate dehydrogenase (LDH) and creatine phosphokinase (CPK), and increased biomarkers of renal injury, urea and creatinine. DZN also increased hepatic, renal, and cardiac lipid peroxidation and decreased antioxidant biomarker levels. SO and (or) ALA supplementation ameliorated the deleterious effects of DZN intoxication. Treatment improved hematology and serum parameters, enhanced endogenous antioxidant status, and reduced lipid peroxidation. Importantly, they exerted synergistic hepatoprotective, nephroprotective, and cardioprotective effects. Our findings demonstrate that SO and (or) ALA supplementation can alleviate the toxic effects of DZN via their potent antioxidant and free radical-scavenging activities.
authors.affiliation: a Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Abdel-Daim
authors.affiliation: b Department of Clinical Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Ramadan
authors.initials: R
authors.lastname: Taha
authors.affiliation: c Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
authors.forename: Emad W
authors.initials: EW
authors.lastname: Ghazy
authors.affiliation: d Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
authors.forename: Yasser S
authors.initials: YS
authors.lastname: El-Sayed
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D015415:Biomarkers / Q000378:metabolism; D002316:Cardiotonic Agents / Q000008:administration & dosage; D003976:Diazinon / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004357:Drug Synergism; D016166:Free Radical Scavengers / Q000008:administration & dosage; D006321:Heart / Q000187:drug effects; D007306:Insecticides / Q000037:antagonists & inhibitors / Q000633:toxicity; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000008:administration & dosage*; D008063:Thioctic Acid / Q000008:administration & dosage*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002316:Cardiotonic Agents; D016166:Free Radical Scavengers; D007306:Insecticides; D008063:Thioctic Acid; D012715:Sesame Oil; D003976:Diazinon
keywords: acide lipoïque; antioxidant; antioxydant; diazinon; huile de sésame; lipoic acid; oxidative stress; sesame oil; stress oxydatif