title: LONG-TERM CONSERVATION OF PROTOCORMS OF Brassavola nodosa (L) LIND. (ORCHIDACEAE): EFFECT OF ABA AND A RANGE OF CRYOCONSERVATION TECHNIQUES.
abstract: BACKGROUND
  Populations of Brassavola nodosa have been severely affected by habitat destruction and illegal collecting, and as with the majority of orchid species, it is critical to take action to guarantee their continued survival.
  
  
  OBJECTIVE
  The present study aimed to establish protocols for the long-term conservation of protocorms of species.
  
  
  METHODS
  Four different cryogenic techniques were compared: encapsulation-dehydration (ED), encapsulation-vitrification (EV), encapsulation-dehydration-vitrification (EDV) and vitrification.
  
  
  RESULTS
  Preculture of protocorms with ABA was a critical factor in obtaining high percentages of regrowth. With vitrification, 100% regrowth was achieved in five treatments, mainly when protocorms were dehydrated with PVS2 for 120 min. 100% regrowth was also obtained with EDV, where the protocorms were precultured with ABA 5 mg/l for 3 days and incubated with PVS2 for 60 min. With the ED, regrowth of 72% was achieved with the preculture of protocorms with ABA 5 mg/l for the three times of incubation used (3, 6 and 9 days). In the case of EV, 92% regrowth, was recorded when protocorms were precultured for 9 days with ABA 3 mg/l and incubated with PVS2 for 90 min.
  
  
  CONCLUSIONS
  Although regrowth of protocorms was obtained with all the techniques used, the vitrification technique is preferred since it requires less labour and is less costly.
authors.affiliation: Red Manejo Biotecnologico de Recursos, Instituto de Ecologia, Mexico. martin.mata@inecol.mx.
authors.forename: M
authors.initials: M
authors.lastname: Mata-Rosas
authors.affiliation: Red Manejo Biotecnologico de Recursos, Instituto de Ecologia, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Lastre-Puertos
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003890:Desiccation / Q000379:methods*; D029595:Orchidaceae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D000040:Abscisic Acid