title: Next generation breeding.
abstract: The genomic revolution of the past decade has greatly improved our understanding of the genetic make-up of living organisms. The sequencing of crop genomes has completely changed our vision and interpretation of genome organization and evolution. Re-sequencing allows the identification of an unlimited number of markers as well as the analysis of germplasm allelic diversity based on allele mining approaches. High throughput marker technologies coupled with advanced phenotyping platforms provide new opportunities for discovering marker-trait associations which can sustain genomic-assisted breeding. The availability of genome sequencing information is enabling genome editing (site-specific mutagenesis), to obtain gene sequences desired by breeders. This review illustrates how next generation sequencing-derived information can be used to tailor genomic tools for different breeders' needs to revolutionize crop improvement.
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Genomics Research Centre, Via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.
authors.forename: Delfina
authors.initials: D
authors.lastname: Barabaschi
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Genomics Research Centre, Via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Tondelli
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Genomics Research Centre, Via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Desiderio
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Rice Research Unit, SS 11 to Torino Km 2.5, 13100 Vercelli, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Volante
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Research Unit for Cereal Selection in Continental areas, via R. Forlani, e, 26866 S. Angelo Lodigiano, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Vaccino
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Rice Research Unit, SS 11 to Torino Km 2.5, 13100 Vercelli, Italy.
authors.forename: Giampiero
authors.initials: G
authors.lastname: Valè
authors.affiliation: Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Genomics Research Centre, Via San Protaso 302, 29017 Fiorenzuola d'Arda, Italy. Electronic address: luigi.cattivelli@entecra.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Cattivelli
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D018556:Crops, Agricultural / Q000145:classification / Q000235:genetics*; D056726:Genetic Association Studies / Q000379:methods; D014644:Genetic Variation; D018745:Genome, Plant / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D000069600:Plant Breeding / Q000379:methods*; D040641:Quantitative Trait Loci / Q000235:genetics
keywords: Allele diversity; Genome editing; Genomic selection; Molecular markers; Next generation sequencing; Plant breeding