title: Spectral Reflectance Properties of Gossypium hirsutum Leaves after Heavy Metal Toxicity.
abstract: The study demonstrated a link between heavy metal induced stress and optical properties of the leaves of Gossypium hirsutum. This work was conducted using the pot culture experiment. Three replications and four concentrations of Chromium (5%, 10%, 20% and 35%) with a control to assess the growth of plants were used. This experiment was conducted till 80 days. On 80th day the experiment was terminated and the leaves were plucked and air-dried. Portion of the air-dried, powdered cotton leaves was used for the Fourier Transformed Infra-Red (FT-IR) spectral analysis and the remaining portion was used for analyzing the biochemical constituents of the leaves (chlorophyll a, b, and total C and N contents). The Fourier Transformed Infra-Red spectrum indicated the aliphatic alcohol, inorganic bridge, C-OH stretch, C-H deformation, aromatic C=C stretch, OH stretch of H bond, aliphatic C-H stretch and OH stretch of aromatic C-H. The shifting and overlapping of the spectrum was observed after heavy metal induced toxicity when compared with that of the control spectra. This was supported by the biochemical analysis of chlorophyll a, b, and total carbon and nitrogen contents. This method was a preliminary study, which may be instrumental in developing optimal procedures for interpreting a particular stress of plants through analysis of their leaf reflectance spectrum.
authors.forename: K K
authors.initials: KK
authors.lastname: Manjuladevi
authors.forename: S
authors.initials: S
authors.lastname: Suriyanarayanan
authors.forename: S
authors.initials: S
authors.lastname: Balasubramanian
mesh_terms: D002244:Carbon / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis; D002857:Chromium / Q000633:toxicity*; D003368:Gossypium / Q000737:chemistry / Q000187:drug effects*; D009584:Nitrogen / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects*; D058998:Remote Sensing Technology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002857:Chromium; D002734:Chlorophyll; D002244:Carbon; D009584:Nitrogen