title: Pharmacokinetics of sarafloxacin in allogynogenetic silver crucian carp, Carassius auratus gibelio.
abstract: The pharmacokinetic properties of sarafloxacin were investigated after single intravenous (i.v.) and oral (p.o.) administration of 10 mg/kg body weight (b.w.) in allogynogenetic silver crucian carp at 24-26 °C. The plasma concentrations of sarafloxacin were determined by high-performance liquid chromatography. After i.v. administration, the plasma concentration-time data were described by an open two-compartment model. The elimination half-life (T(1/2β)) was estimated to be 22.58 h. The volume of distribution, V(d(area)), was estimated to be 5.95 L/kg, indicating good tissue penetration of sarafloxacin in the fish. Area under the concentration-time curve (AUC) and total body clearance of sarafloxacin were 56.86 µg·h/mL and 0.18 L/h/kg, respectively. Following p.o. administration, the maximum plasma concentration (C(max)), T(1/2β), and AUC of sarafloxacin were 0.79 µg/mL, 46.68 h, and 16.58 µg·h/mL, respectively. Absorption of the drug was not good with a bioavailability (F) of 29.15%. Based on a minimum inhibitory concentration (MIC) of 0.00625 to 0.045 μg/mL for susceptible strains, sarafloxacin p.o. administration at a dose 10 mg/kg could be efficacious against common pathogenic bacteria of fish.
authors.affiliation: Department of Economical Animal Sciences and Aquaculture, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Economical Animal Sciences and Aquaculture, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Economical Animal Sciences and Aquaculture, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. fangxx@njau.edu.cn.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Liu
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001682:Biological Availability; D002347:Carps / Q000097:blood / Q000378:metabolism*; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D008954:Models, Biological
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; C047224:sarafloxacin
keywords: Allogynogenetic silver crucian carp; Intravenous; Oral; Pharmacokinetics; Plasma; Sarafloxacin