title: Haloimpatiens lingqiaonensisgen. nov., sp. nov., an anaerobic bacterium isolated from paper-mill wastewater.
abstract: An anaerobic bacterium, strain ZC-CMC3T, was isolated from a wastewater sample in Zhejiang, China. Cells were Gram-stain-positive, peritrichous, non-spore-forming, rod-shaped (0.6-1.2 × 2.9-5.1 μm) and catalase- and oxidase-negative. Strain ZC-CMC3Twas able to grow at 25-48 °C (optimum 43 °C) and pH 5.5-8.0 (optimum pH 7.0). The NaCl concentration range for growth was 0-3 % (w/v) (optimum 0 %). The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylglycerol, several phospholipids and glycolipids. Main fermentation products from PYG medium were formate, acetate, lactate and ethanol. Substrates which could be utilized were peptone, tryptone, yeast extract and beef extract. No respiratory quinone was detected. The main fatty acids were C14 : 0, C16 : 0, C16 : 1cis7 and C16 : 1cis9. The DNA G+C content was 30.0 mol%. 16S rRNA gene sequence analysis revealed that the isolate belonged to the familyClostridiaceae. Phylogenetically, the most closely related species wereOceanirhabdus sediminicolaNH-JN4T(92.8 % 16S rRNA gene sequence similarity) andClostridium tepidiprofundiSG 508T(92.6 %). On the basis of phylogenetic, chemotaxonomic and phenotypic characteristics, strain ZC-CMC3Trepresents a novel species of a new genus in the familyClostridiaceae,for which the nameHaloimpatiens lingqiaonensisgen. nov., sp. nov. is proposed. The type strain of the type species is ZC-CMC3T( = KCTC 15321T= JCM 19210T= CCTCC AB 2013104T).
authors.affiliation: Department of Biology, Xinjiang Normal University, Urumqi 830054, PR China.|College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Dildar
authors.initials: D
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Nai-Fang
authors.initials: NF
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Wen-Wu
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Shuai-Bo
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Biology, Xinjiang Normal University, Urumqi 830054, PR China.
authors.forename: Dilbar
authors.initials: D
authors.lastname: Th
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu