title: Peptide-conjugated hyaluronic acid surface for the culture of human induced pluripotent stem cells under defined conditions.
abstract: Hyaluronic acid (HA) has been cross-linked to form hydrogel for potential applications in the self-renewal and differentiation of human pluripotent stem cells (hPSCs) for years. However, HA hydrogel with improved residence time and mechanical integrity that allows the survival of hPSCs under defined conditions is still much needed for clinical applications. In this study, HA was modified with methacrylate functional groups (MeHA) and cross-linked by photo-crosslinking method. After subsequent conjugation with adhesive peptide, these MeHA surfaces demonstrated performance in facilitating human induced pluripotent stem cells (hiPSCs) proliferation, and good pluripotency maintenance of hiPSCs under defined conditions. Moreover, MeHA films on glass-slides exhibited long residence time and mechanical stability throughout hiPSC culture. Our photo-crosslinkable MeHA possesses great value in accelerating the application of HA hydrogel in hiPSCs proliferation and differentiation with the conjugation of adhesive peptides.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhangxiaohong@pku.edu.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhp07@pku.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhaoyinghui1987@gmail.com.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China. Electronic address: 874912103@qq.com.
authors.forename: Mengke
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: sc-wei@pku.edu.cn.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Wei
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects*; D008689:Methacrylates / Q000737:chemistry; D019096:Vitronectin / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; D019096:Vitronectin; D006820:Hyaluronic Acid
keywords: 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (PubChem CID: 15908); Defined; Human induced pluripotent stem cell; Hyaluronic acid; Methacryloyl anhydride (PubChem CID: 12974); N-hydroxysuccinimide (PubChem CID: 80170); Peptide; Sodium hyaluronate (PubChem CID: 3084049)