title: Effect of genistein added to bull semen after thawing on pronuclear and sperm quality.
abstract: The aim of this research was to study the effect of different genistein treatments on bull sperm after thawing on pronuclear formation after in vitro fertilization (IVF) and on different sperm quality variables. Three experiments were performed. In Experiment 1, three treatments (Control, sperm incubation for 1h at 37 °C with or without genistein) and two sperm concentrations during IVF (1 or 3 × 10(6)sperm/mL) were evaluated to study the influence of genistein on pronuclear formation (PNF). Sperm incubation for 1h before IVF reduced PNF regardless of sperm concentration. However, after sperm incubation and with 3 × 10(6)sperm/mL in IVF, the genistein treatment group had greater fertilization rates than the untreated group. In Experiment 2, six treatments plus the control group were performed to study the effect of genistein (presence or not) and incubation conditions (30 min at 37 °C, 1h at 27 °C or at 37 °C) on PNF using 3 × 10(6)sperm/mL for IVF. When incubation time was reduced to 30 min, PNF rate from the genistein treatment group was no different from either the control group or in the group in which incubation occurred for 1h at 27 °C. In Experiment 3, the effect of several genistein treatments (control; genistein treatment for 30 min of incubation at 37 °C; genistein treatment for 1h of incubation at 27 °C) on sperm motility, viability and DNA fragmentation were evaluated. Genistein did not improve sperm motility and, depending on the experimental group or time, it either reduced or had no effect on sperm motility. Genistein treatment did not improve sperm viability after 5h of incubation. However, genistein treatment for 1h at 27 °C decreased sperm DNA fragmentation compared with the control group after 5h of sperm incubation. In conclusion, the treatment of bull sperm with genistein for 1h at 27 °C could decrease sperm DNA fragmentation, although PNF rate after IVF and sperm motility were reduced.
authors.affiliation: Departamento de Biología Funcional y Antropología Física, Universitat de València, Burjassot, 46100 Valencia, Spain; Centro de Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo 187, Pol. La Esperanza n°100, 12400 Segorbe, Castellón, Spain. Electronic address: miguel.silvestre@uv.es.
authors.forename: M A
authors.initials: MA
authors.lastname: Silvestre
authors.affiliation: TECNOGAM Research Group, Instituto Universitario de Ciencias Ambientales (IUCA), Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Huesca, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Vicente-Fiel
authors.affiliation: Centro de Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo 187, Pol. La Esperanza n°100, 12400 Segorbe, Castellón, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Raga
authors.affiliation: Centro de Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo 187, Pol. La Esperanza n°100, 12400 Segorbe, Castellón, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Salvador
authors.affiliation: Departamento de Biología Funcional y Antropología Física, Universitat de València, Burjassot, 46100 Valencia, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Soler
authors.affiliation: TECNOGAM Research Group, Instituto Universitario de Ciencias Ambientales (IUCA), Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Huesca, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Yániz
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D053938:DNA Fragmentation / Q000187:drug effects; D005306:Fertilization; D005307:Fertilization in Vitro / Q000662:veterinary; D019833:Genistein / Q000494:pharmacology*; D008297:Male; D009865:Oocytes; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D012661:Semen / Q000737:chemistry / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000379:methods; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013696:Temperature; D013997:Time Factors
chemical_list: D047428:Protein Kinase Inhibitors; D019833:Genistein
keywords: DNA fragmentation; Genistein; Motility; Pronuclear formation; Viability