title: Murine Kidney Transplant Technique.
abstract: The first mouse kidney transplant technique was published in 1973(1) by the Russell laboratory. Although it took some years for other labs to become proficient in and utilize this technique, it is now widely used by many laboratories around the world. A significant refinement to the original technique using the donor aorta to form the arterial anastomosis instead of the renal artery was developed and reported in 1993 by Kalina and Mottram (2) with a further advancement coming from the same laboratory in 1999 (3). While one can become proficient in this model, a search of the literature reveals that many labs still experience a high proportion of graft loss due to arterial thrombosis. We describe here a technique that was devised in our laboratory that vastly reduces the arterial thrombus reported by others (4,5). This is achieved by forming a heel-and-toe cuff of the donor infra-renal aorta that facilitates a larger anastomosis and straighter blood flow into the kidney.
authors.affiliation: Colorado Center for Transplantation Care, Research and Education, University of Colorado, Denver; Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado, Denver; robert.plenter@ucdenver.edu.
authors.forename: Robert
authors.initials: R
authors.lastname: Plenter
authors.affiliation: Department of Medicine, Division of Renal Diseases and Hypertension, Medical Center and University of Colorado, Denver.
authors.forename: Swati
authors.initials: S
authors.lastname: Jain
authors.affiliation: Department of Surgery, Division of Transplant Surgery, University of Colorado School of Medicine, University of Colorado-Denver.
authors.forename: Chelsea M
authors.initials: CM
authors.lastname: Ruller
authors.affiliation: Department of Surgery, Division of Transplant Surgery, University of Colorado School of Medicine, University of Colorado-Denver.
authors.forename: Trevor L
authors.initials: TL
authors.lastname: Nydam
authors.affiliation: Department of Medicine, Division of Renal Diseases and Hypertension, Medical Center and University of Colorado, Denver; Renal Section, Denver Veterans Affairs Medical Center.
authors.forename: Alkesh H
authors.initials: AH
authors.lastname: Jani
mesh_terms: D000818:Animals; D001163:Arteriovenous Anastomosis / Q000601:surgery*; D006439:Hemodynamics; D007668:Kidney / Q000098:blood supply / Q000601:surgery*; D016030:Kidney Transplantation / Q000379:methods / Q000662:veterinary*; D051379:Mice; D014656:Vascular Surgical Procedures