title: Temporal and organ-specific detection of cNMPs including cUMP in the zebrafish.
abstract: The cyclic pyrimidine nucleotides cCMP and cUMP occur in mammalian cell lines. Recently, cCMP was also identified in mouse organs. Due to technical difficulties, it has not been possible to detect cUMP in organs or tissues yet. Here, we have generated a temporal profile of the occurrence of nucleoside 3',5'-cyclic monophosphates during different developmental stages of embryogenesis and in different organs of the adult zebrafish Danio rerio. Cyclic nucleotides were quantified by high performance liquid chromatography quadrupole tandem mass spectrometry. The identity of cCMP and cUMP in the zebrafish was confirmed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry. We show for the first time that cUMP can be detected during embryogenesis and in adult organs of this vertebrate model system.
authors.affiliation: Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: dittmar.fanni@mh-hannover.de.
authors.forename: Fanni
authors.initials: F
authors.lastname: Dittmar
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, D-14476 Potsdam, Germany; Institute of Molecular Biology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: salim.seyfried@uni-potsdam.de.
authors.forename: Salim
authors.initials: S
authors.lastname: Abdelilah-Seyfried
authors.affiliation: Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany; Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: tschirner.sarah@mh-hannover.de.
authors.forename: Sarah K
authors.initials: SK
authors.lastname: Tschirner
authors.affiliation: Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany; Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: kaever.volkhard@mh-hannover.de.
authors.forename: Volkhard
authors.initials: V
authors.lastname: Kaever
authors.affiliation: Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany. Electronic address: seifert.roland@mh-hannover.de.
authors.forename: Roland
authors.initials: R
authors.lastname: Seifert
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D003563:Cyclic CMP / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D009928:Organ Specificity / Q000502:physiology; D014018:Tissue Distribution; D014542:Uridine Monophosphate / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*
chemical_list: D009712:Nucleotides, Cyclic; D003563:Cyclic CMP; C045338:cyclic 3',5'-uridine monophosphate; D014542:Uridine Monophosphate
keywords: Cyclic CMP; Cyclic UMP; Zebrafish