title: miRNA profiling in serum and tissue samples to assess noninvasive biomarkers for NSCLC clinical outcome.
abstract: In NSCLC, the altered expression of some miRNAs in primary tumor tissues has been correlated with diagnosis and prognosis, while the role of circulating miRNAs as cancer biomarkers is currently emerging. MiRNA expression profile through miRNA Affymetrix array was evaluated on a training set formed by the tumor component (n = 30 NSCLC serum, n = 11/30 tumor tissues) and the control component (n = 10 healthy serum and n = 11/30 noncancerous counterparts). Statistical analyses highlighted the following: a = 55 miRNAs deregulated in tumor serum, b = 27 miRNAs deregulated in tumor tissues, and c = 2 miRNAs deregulated both in tumor serum and in tumor tissues. MiRwalk tool and enrichment pathway analyses selected some miRNAs whose target genes are correlated with the main pathways involved in NSCLC tumorigenesis. The altered expression of the selected miR-486-5p (a), miR-29c* (b), and miR-133a (c) was confirmed in the validation set (n = 40). MiR-486-5p had a higher expression in tumor serum than in tumor tissues (P = 0.004), and miR-29c* showed a lower expression in tumor tissues than in tumor serum (P < 0.001). MiR-133a had a not different expression in both tumor serum and tumor tissues (P = 0.07). The low level of miR-486-5p expression in the serum of affected patients was associated with a worse time to progression of disease (P = 0.010), and serum level of miR-486-5p was a significant prognostic indicator of NSCLC (adjusted hazard ratio = 0.179, P = 0.019). These data suggest the possibility to monitor affected patients through serum and/or tissue samples, analyzing the altered expression of specific miRNAs, in order to detect prognostic biomarkers in the NSCLC.
authors.affiliation: Molecular Genetics Laboratory, IRCCS, Istituto Tumori "Giovanni Paolo II", v.le Orazio Flacco 65, 70124, Bari, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Petriella
authors.affiliation: Molecular Genetics Laboratory, IRCCS, Istituto Tumori "Giovanni Paolo II", v.le Orazio Flacco 65, 70124, Bari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: De Summa
authors.affiliation: Molecular Genetics Laboratory, IRCCS, Istituto Tumori "Giovanni Paolo II", v.le Orazio Flacco 65, 70124, Bari, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Lacalamita
authors.affiliation: Medical Oncology Unit, IRCCS, Istituto Tumori "Giovanni Paolo II", Bari, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Galetta
authors.affiliation: Medical Oncology Unit, IRCCS, Istituto Tumori "Giovanni Paolo II", Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Catino
authors.affiliation: Medical Oncology Unit, IRCCS, Istituto Tumori "Giovanni Paolo II", Bari, Italy.
authors.forename: A F
authors.initials: AF
authors.lastname: Logroscino
authors.affiliation: Medical Genetics Unit, IRCCS "Casa Sollievo della Sofferenza", San Giovanni Rotondo, FG, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Palumbo
authors.affiliation: Medical Genetics Unit, IRCCS "Casa Sollievo della Sofferenza", San Giovanni Rotondo, FG, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Carella
authors.affiliation: Pathology Unit "S.-Paolo" Hospital, Bari, Italy.
authors.forename: F A
authors.initials: FA
authors.lastname: Zito
authors.affiliation: Pathology Unit, IRCCS, Istituto Tumori "Giovanni Paolo II", Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.affiliation: Molecular Genetics Laboratory, IRCCS, Istituto Tumori "Giovanni Paolo II", v.le Orazio Flacco 65, 70124, Bari, Italy. s.tommasi@oncologico.bari.it.
authors.forename: S
authors.initials: S
authors.lastname: Tommasi
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D063646:Carcinogenesis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D046228:Microarray Analysis; D008875:Middle Aged; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: Biomarkers; Circulating miRNAs; MicroRNAs; Non small cell lung cancer; Serum