title: Matrix Metalloproteinases in Primary Culture of Cardiomyocytes.
abstract: The highly organized contractile apparatus of cardiomyocytes in heart tissue allows for their continuous contractility, whereas extracellular matrix components are synthesized and spatially organized by fibroblasts and endothelial cells. However, reorganization of the cardiomyocyte contractile apparatus occurs upon their 2D cultivation, which is accompanied by transient loss of their contractility and acquired capability of extracellular matrix synthesis (Bildyug, N. B., and Pinaev, G. P. (2013) Tsitologiya, 55, 713-724). In this study, matrix metalloproteinases were investigated at different times of cardiomyocyte 2D cultivation and 3D cultivation in collagen gels. It was found that cardiomyocytes in 2D culture synthesize matrix metalloproteinases MMP-2 and MMP-9, wherein their amount varies with the cultivation time. The peak MMP-9 amount is at early cultivation time, when the reorganization of cardiomyocyte contractile apparatus occurs, and the MMP-2 peak precedes the recovery of the initial organization of their contractile apparatus. Upon cardiomyocyte cultivation in 3D collagen gels, in which case their contractile apparatus does not rearrange, a steady small amount of MMP-2 and MMP-9 is observed. These data indicate that the cardiomyocyte contractile apparatus reorganization in culture is associated with synthesis and spatial organization of their own extracellular matrix.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, Russia. relapse@yandex.ru.
authors.forename: N B
authors.initials: NB
authors.lastname: Bildyug
authors.forename: I V
authors.initials: IV
authors.lastname: Voronkina
authors.forename: L V
authors.initials: LV
authors.lastname: Smagina
authors.forename: N M
authors.initials: NM
authors.lastname: Yudintseva
authors.forename: G P
authors.initials: GP
authors.lastname: Pinaev
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis / Q000378:metabolism*; D009200:Myocardial Contraction / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D051381:Rats; D013997:Time Factors
chemical_list: D003094:Collagen; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9