title: Sevoflurane aggregates cognitive dysfunction and hippocampal oxidative stress induced by β-amyloid in rats.
abstract: OBJECTIVE
  To investigate the effects of sevoflurane inhalation on β-amyloid (Aβ)-induced cognitive disorders and hippocampal oxidative stress in rat models.
  
  
  METHODS
  Cognitive dysfunction is induced by hippocampal injection of Aβ1-40 (10μg in 2μl) for 22days. To explore the effect of sevoflurane inhalation on Aβ1-40 induced cognitive disorder, two doses of sevoflurane inhalation are used: 1.3% (Aβ+S1) and 2.6% (Aβ+S2). Sham operation (Sham, for operation control), saline injection (Control, for injection control) and 30% oxygen inhalation after Aβ1-40 injection (Aβ+O2, for inhalation control) were used as controls. All rats were further tested in electrical Y-maze and Morris water maze. Serum S100β levels, hippocampal superoxide dismutase (SOD) activity, S100β expression and malonyldialdehyde (MDA) concentrations were further quantified.
  
  
  RESULTS
  Rats in Aβ+O2, Aβ+S1 and Aβ+S2 groups had lower number of correct actions in the electrical Y maze task, longer escape latencies, less time exploring the original platform, elevated serum S100β levels, depressed hippocampal SOD activity, S100β expression and higher MDA concentrations compared to control group (p<0.05). Such difference was not significant between Aβ+S1 and Aβ+O2 rats. Rats in Aβ+S2 group, however, showed significantly impaired performances compared to those in Aβ+S1 group (p<0.05).
  
  
  CONCLUSIONS
  Sevoflurane (2.6%) can aggravate the Aβ-induced cognitive dysfunction, possibly via the intracerebral oxidative stress response.
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yue
authors.affiliation: Department of Pharmacy, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Shanbin
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Ling
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, China. Electronic address: zhaop@sj-hospital.org.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ping
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D003072:Cognition Disorders / Q000139:chemically induced* / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008738:Methyl Ethers / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane
chemical_list: D016229:Amyloid beta-Peptides; D008738:Methyl Ethers; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D000077149:Sevoflurane
keywords: Amyloid protein; Cognitive dysfunctions; Oxidative stress; Sevoflurane inhalation