title: Spectroscopic studies of interaction between CuO nanoparticles and bovine serum albumin.
abstract: Recently, the great interests in manufacturing and application of metal oxide nanoparticles in commercial and industrial products have led to focus on the potential impact of these particles on biomacromolecules. In the present study, the interaction of copper oxide (CuO) nanoparticles with bovine serum albumin (BSA) was studied by spectroscopic techniques. The zeta potential value for BSA and CuO nanoparticles with average diameter of around 50 nm at concentration of 10 μM in the deionized (DI) water were -5.8 and -22.5 mV, respectively. Circular dichroism studies did not show any changes in the content of secondary structure of the protein after CuO nanoparticles interaction. Fluorescence data revealed that the fluorescence quenching of BSA by CuO nanoparticles was the result of the formed complex of CuO nanoparticles - BSA. Binding constants and other thermodynamic parameters were determined at three different temperatures. The hydrogen bond interactions are the predominant intermolecular forces to stabilize the CuO nanoparticle - BSA complex. This study provides important insight into the interaction of CuO nanoparticles with proteins, which may be of importance for further application of these nanoparticles in biomedical applications.
authors.affiliation: a Department of Biomedical Engineering, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Paniz
authors.initials: P
authors.lastname: Esfandfar
authors.affiliation: b Department of Nanotechnology , Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch , Islamic Azad University (IAUPS) , Tehran , Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Falahati
authors.affiliation: c Institute of Biochemistry and Biophysics, University of Tehran , Tehran , Iran.
authors.forename: AliAkbar
authors.initials: A
authors.lastname: Saboury
mesh_terms: D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D012710:Serum Albumin, Bovine; D003300:Copper; C000520:cuprous oxide
keywords: BSA; CuO nanoparticles; circular dichroism; fluorescence; interaction