title: The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals.
abstract: We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
authors.forename: Hao-Ying
authors.initials: HY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
authors.forename: Bao-Ling
authors.initials: BL
authors.lastname: Qi
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China. bfyuan@whu.edu.cn yqfeng@whu.edu.cn.
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry* / Q000378:metabolism / Q000031:analogs & derivatives; D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D019175:DNA Methylation; D008322:Mammals; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D012313:RNA / Q000737:chemistry* / Q000378:metabolism
chemical_list: C560973:5-formylcytosine; C011865:5-hydroxymethylcytosine; D012313:RNA; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA