title: Functional analysis of alcohol dehydrogenase (ADH) genes in Pichia pastoris.
abstract: OBJECTIVE
  To characterize the genes responsible for ethanol utilization in Pichia pastoris.
  
  
  RESULTS
  ADH3 (XM_002491337) and ADH (FN392323) genes were disrupted in P. pastoris. The ADH3 mutant strain, MK115 (Δadh3), lost its ability to grow on minimal ethanol media but produced ethanol in minimal glucose medium. ADH3p was responsible for 92 % of total Adh enzyme activity in glucose media. The double knockout strain MK117 (Δadh3Δadh) also produced ethanol. The Adh activities of X33 and MK116 (Δadh) strains were not different. Thus, the ADH gene does not play a role in ethanol metabolism.
  
  
  CONCLUSIONS
  The PpADH3 is the only gene responsible for consumption of ethanol in P. pastoris.
authors.affiliation: Department of Food Engineering, Akdeniz University, Dumlupinar Bulvari Campus, 07058, Antalya, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Karaoglan
authors.affiliation: Department of Food Engineering, Akdeniz University, Dumlupinar Bulvari Campus, 07058, Antalya, Turkey.
authors.forename: Fidan Erden
authors.initials: FE
authors.lastname: Karaoglan
authors.affiliation: Department of Food Engineering, Akdeniz University, Dumlupinar Bulvari Campus, 07058, Antalya, Turkey. minan@akdeniz.edu.tr.|Food Safety and Agricultural Research Center, Akdeniz University, 07058, Antalya, Turkey. minan@akdeniz.edu.tr.
authors.forename: Mehmet
authors.identifier: http://orcid.org/0000-0003-1806-7927
authors.initials: M
authors.lastname: Inan
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D055786:Gene Knockout Techniques; D010843:Pichia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D003470:Culture Media; D000431:Ethanol; D000426:Alcohol Dehydrogenase
keywords: Alcohol dehydrogenase; Crabtree yeast; Ethanol metabolism; Methylotrophic yeast; Pichia pastoris; PpADH; PpADH3