title: Structural characterization of human heparanase reveals insights into substrate recognition.
abstract: Heparan sulfate (HS) is a glycosaminoglycan that forms a key component of the extracellular matrix (ECM). Breakdown of HS is carried out by heparanase (HPSE), an endo-β-glucuronidase of the glycoside hydrolase 79 (GH79) family. Overexpression of HPSE results in breakdown of extracellular HS and release of stored growth factors and hence is strongly linked to cancer metastasis. Here we present crystal structures of human HPSE at 1.6-Å to 1.9-Å resolution that reveal how an endo-acting binding cleft is exposed by proteolytic activation of latent proHPSE. We used oligosaccharide complexes to map the substrate-binding and sulfate-recognition motifs. These data shed light on the structure and interactions of a key enzyme involved in ECM maintenance and provide a starting point for the design of HPSE inhibitors for use as biochemical tools and anticancer therapeutics.
authors.affiliation: Department of Chemistry, University of York, York, UK.
authors.forename: Liang
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Chemistry, University of York, York, UK.
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Viola
authors.affiliation: Department of Chemistry, University of York, York, UK.
authors.forename: Andrzej M
authors.initials: AM
authors.lastname: Brzozowski
authors.affiliation: Department of Chemistry, University of York, York, UK.
authors.forename: Gideon J
authors.identifier: http://orcid.org/0000-0002-7343-776X
authors.initials: GJ
authors.lastname: Davies
mesh_terms: D018360:Crystallography, X-Ray; D005966:Glucuronidase / Q000737:chemistry* / Q000378:metabolism*; D006497:Heparan Sulfate / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011487:Protein Conformation; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D006497:Heparan Sulfate; D000099175:Heparanase