title: Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism.
abstract: Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast. We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization. In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1. The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth. These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts.
authors.affiliation: Department of Pharmacy, Guangdong Medical College, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China.
authors.forename: Chong-Chong
authors.initials: CC
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, Guangdong Medical College, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Guangdong Medical College, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Fu
authors.affiliation: Department of Pharmacy, Sun Yat-Sen Memorial Hospital, Guangzhou 510120, China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Zhu
authors.affiliation: Department of Pharmacy, Guangdong Medical College, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China.
authors.forename: Yan-Xing
authors.initials: YX
authors.lastname: Zhou
authors.affiliation: Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Department of Pharmacy, Guangdong Medical College, No. 1, Xincheng Dadao, Songshan Lake Science and Technology Industry Park, Dongguan 523808, China.
authors.forename: Zhi-Kun
authors.initials: ZK
authors.lastname: Zhou