title: DNA Triplexes That Bind Several Cofactor Molecules.
abstract: Cofactors are critical for energy-consuming processes in the cell. Harnessing such processes for practical applications requires control over the concentration of cofactors. We have recently shown that DNA triplex motifs with a designed binding site can be used to capture and release nucleotides with low micromolar dissociation constants. In order to increase the storage capacity of such triplex motifs, we have explored the limits of ligand binding through designed cavities in the oligopurine tract. Oligonucleotides with up to six non-nucleotide bridges between purines were synthesized and their ability to bind ATP, cAMP or FAD was measured. Triplex motifs with several single-nucleotide binding sites were found to bind purines more tightly than triplexes with one large binding site. The optimized triplex consists of 59 residues and four C3-bridges. It can bind up to four equivalents of ligand with apparent Kd values of 52 µM for ATP, 9 µM for FAD, and 2 µM for cAMP. An immobilized version fuels bioluminescence via release of ATP at body temperature. These results show that motifs for high-density capture, storage and release of energy-rich biomolecules can be constructed from synthetic DNA.
authors.affiliation: Institut für Organische Chemie, Universität Stuttgart, 70569 Stuttgart (Germany), Fax: (+49) 711-685-64321.
authors.forename: Sven
authors.initials: S
authors.lastname: Vollmer
authors.affiliation: Institut für Organische Chemie, Universität Stuttgart, 70569 Stuttgart (Germany), Fax: (+49) 711-685-64321. lehrstuhl-2@oc.uni-stuttgart.de.
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D001665:Binding Sites; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011687:Purines / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D009841:Oligonucleotides; D011687:Purines; C051802:triplex DNA; D005182:Flavin-Adenine Dinucleotide; D000255:Adenosine Triphosphate; D004247:DNA; C030985:purine
keywords: DNA; cofactors; nucleotide storage; oligonucleotides; triplexes