title: Chemically induced acute model of sarcosinemia in wistar rats.
abstract: In the present study, we developed an acute chemically induced model of sarcosinemia in Wistar rats. Wistar rats of 7, 14 and 21 postpartum days received sarcosine intraperitoneally in doses of 0.5 mmol/Kg of body weight three time a day at intervals of 3 h. Control animals received saline solution (NaCl 0.85 g%) in the same volume (10 mL/Kg of body weight). The animals were killed after 30 min, 1, 2, 3 or 6 h after the last injection and the brain and the blood were collected for sarcosine measurement. The results showed that plasma and brain sarcosine concentrations achieved levels three to four times higher than the normal levels and decreased in a time-dependent way, achieving normal levels after 6 hours. Considering that experimental animal models are useful to investigate the pathophysiology of human disorders, our model of sarcosinemia may be useful for the research of the mechanisms of neurological dysfunction caused by high tissue sarcosine levels.
authors.affiliation: PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil.
authors.forename: Rodrigo Binkowski
authors.initials: RB
authors.lastname: de Andrade
authors.affiliation: PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil.
authors.forename: Tanise
authors.initials: T
authors.lastname: Gemelli
authors.affiliation: PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil.
authors.forename: Denise Bertin
authors.initials: DB
authors.lastname: Rojas
authors.affiliation: PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil.
authors.forename: Carlos Severo
authors.initials: CS
authors.lastname: Dutra-Filho
authors.affiliation: PPG Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Rua Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90.035-003, Brazil. clovisdw@ufrgs.br.|Universidade Federal do Rio Grande do Sul, Instituto de Ciências Básicas da Saúde, Departamento de Bioquímica, Rua Ramiro Barcelos 2600-Anexo, Porto Alegre, RS, 90035-003, Brazil. clovisdw@ufrgs.br.
authors.forename: Clovis Milton Duval
authors.initials: CM
authors.lastname: Wannmacher
mesh_terms: D000208:Acute Disease; D000592:Amino Acid Metabolism, Inborn Errors / Q000139:chemically induced*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects*; D004195:Disease Models, Animal; D028361:Mitochondrial Diseases / Q000139:chemically induced*; D017208:Rats, Wistar; D012521:Sarcosine / Q000378:metabolism / Q000494:pharmacology; D050892:Sarcosine Dehydrogenase / Q000172:deficiency*
chemical_list: D050892:Sarcosine Dehydrogenase; D012521:Sarcosine
keywords: Animal model; Metabolic disease; Sarcosine; Sarcosinemia