title: Microarray analysis of circulating micro RNAs in the serum of patients with polymyositis and dermatomyositis reveals a distinct disease expression profile and is associated with disease activity.
abstract: OBJECTIVE
  The aim of this study was a large scale investigation of myositis-associated circulating miRNA molecules and also determination of expression of these candidate molecules in relation to clinical activity of myositis.
  
  
  METHODS
  RNA, containing also miRNAs, was isolated from sera of 28 patients suffering from idiopathic inflammatory myopathies (IIM) and 16 healthy controls. Expression of miRNAs was determined using a miRNA microarray method. Statistical analysis of miRNA expression was carried out using Arraystar software.
  
  
  RESULTS
  Our results showed 23 significantly differentially expressed miRNAs. Six miRNAs were differentially expressed in IIM compared to healthy controls. In dermatomyositis (DM) we found 3 and in polymyositis (PM) 6 differentially expressed miRNAs compared to controls. Three miRNAs were up-regulated in patients with highly active disease compared to patients with low disease activity. Furthermore, we found 26 significantly differentially expressed miRNAs in SLE patients compared to IIM, DM and PM patients.
  
  
  CONCLUSIONS
  This is the first study that comprehensively describes expression levels of circulating miRNAs in serum of patients suffering from IIM. It can be expected that some of these deregulated miRNA molecules are involved in aetiology of IIM and may potentially serve as molecular markers for IIM development or for monitoring of disease activity.
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic. remakova@revma.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Misunova
authors.affiliation: Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Salinas-Riester
authors.affiliation: Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Luthin
authors.affiliation: Department of Developmental Biochemistry, University Medical Center Göttingen, Göttingen, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Pommerenke
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Husakova
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Zavada
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Klein
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Plestilova
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Tana
authors.initials: T
authors.lastname: Svitalkova
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Cepek
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Novota
authors.affiliation: Department of Experimental and Clinical Rheumatology, Institute of Rheumatology, Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Vencovsky
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003882:Dermatomyositis / Q000097:blood / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D012984:Software
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs