title: Trypanosoma cruzi P21: a potential novel target for chagasic cardiomyopathy therapy.
abstract: Chagas disease, which is caused by the parasite Trypanosoma cruzi, is an important cause of cardiomyopathy in Latin America. It is estimated that 10%-30% of all infected individuals will acquire chronic chagasic cardiomyopathy (CCC). The etiology of CCC is multifactorial and involves parasite genotype, host genetic polymorphisms, immune response, signaling pathways and autoimmune progression. Herein we verified the impact of the recombinant form of P21 (rP21), a secreted T. cruzi protein involved in host cell invasion, on progression of inflammatory process in a polyester sponge-induced inflammation model. Results indicated that rP21 can recruit immune cells induce myeloperoxidase and IL-4 production and decrease blood vessels formation compared to controls in vitro and in vivo. In conclusion, T. cruzi P21 may be a potential target for the development of P21 antagonist compounds to treat chagasic cardiomyopathy.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Thaise Lara
authors.initials: TL
authors.lastname: Teixeira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.|Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, SP, Brasil.
authors.forename: Fabrício Castro
authors.initials: FC
authors.lastname: Machado
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Aline
authors.initials: A
authors.lastname: Alves da Silva
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Samuel Cota
authors.initials: SC
authors.lastname: Teixeira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Bruna Cristina
authors.initials: BC
authors.lastname: Borges
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Marlus Alves
authors.initials: MA
authors.lastname: Dos Santos
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Flávia Alves
authors.initials: FA
authors.lastname: Martins
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Paula Cristina
authors.initials: PC
authors.lastname: Brígido
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Adele Aud
authors.initials: AA
authors.lastname: Rodrigues
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Ana Flávia Oliveira
authors.initials: AF
authors.lastname: Notário
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Bruno Antônio
authors.initials: BA
authors.lastname: Ferreira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: João Paulo Silva
authors.initials: JP
authors.lastname: Servato
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Simone Ramos
authors.initials: SR
authors.lastname: Deconte
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Daiana Silva
authors.initials: DS
authors.lastname: Lopes
authors.affiliation: Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Veridiana Melo Rodrigues
authors.initials: VM
authors.lastname: Ávila
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Fernanda de Assis
authors.initials: Fde A
authors.lastname: Araújo
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Tatiana Carla
authors.initials: TC
authors.lastname: Tomiosso
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Marcelo José Barbosa
authors.initials: MJ
authors.lastname: Silva
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brasil.
authors.forename: Claudio Vieira
authors.initials: CV
authors.lastname: da Silva
mesh_terms: D000818:Animals; D009202:Cardiomyopathies / Q000188:drug therapy / Q000209:etiology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D014355:Chagas Disease / Q000188:drug therapy / Q000469:parasitology / Q000473:pathology*; D002633:Chemotaxis / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D015847:Interleukin-4 / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D015800:Protozoan Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology / Q000627:therapeutic use; D014349:Trypanosoma cruzi / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D016207:Cytokines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D015847:Interleukin-4; D009195:Peroxidase