title: [Effects of interleukin 27 and its receptor on TGFβinduced murine pulmonary fibroblast proliferation and transformation].
abstract: OBJECTIVE
  To investigate the effects of interleukin-27 (IL-27) and its receptor (WSX-1) on the proliferation, transformation and collagen synthesis of the mouse lung fibroblasts.
  
  
  METHODS
  Cultured mouse lung fibroblasts were treated with TGF-β1, recombinant murine IL-27, a IL-27 receptor (IL-27R) overexpression vector IL-27R/pCDNA3.1, IL-27 and IL-27R, or all the 3 combined. MTT assay was used to assess the proliferation of the cells, and RT-PCR and Western blotting were employed to examine the mRNA and protein expressions of a-smooth muscle actin (α-SMA) and types I and III collagen; immunofluorescence assay was used to test the expression and location of α-SMA.
  
  
  RESULTS
  TGF-β1 promoted the cell proliferation and obviously enhanced α-SMA expression and types I and III collagen synthesis in the fibroblasts. Both IL-27 and IL-27R significantly inhibited the proliferation of the pulmonary fibroblasts and obviously decreased their α-SMA expression and types I and III collagen synthesis, but when combined,they produced no obvious inhibitory effect on TGF-1-induced proliferation and transformation of pulmonary fibroblasts.
  
  
  CONCLUSIONS
  Both IL-27 and IL-27R alone can suppress the proliferation, transformation, and collagen synthesis of mouse pulmonary fibroblasts, but their combined treatment produces no such inhibitory effect because of the neutralization of exogenous IL-27 by IL-27R to result in the failure of activating the cell signaling pathways.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China. E-mail: 2538287311@qq.com.
authors.forename: Zhao-Xing
authors.initials: ZX
authors.lastname: Dong
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Tai
authors.forename: Wen
authors.initials: W
authors.lastname: Lei
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhen-Kun
authors.initials: ZK
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D007378:Interleukins / Q000494:pharmacology*; D008168:Lung / Q000166:cytology; D051379:Mice; D012333:RNA, Messenger; D018121:Receptors, Cytokine / Q000378:metabolism*; D018123:Receptors, Interleukin; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D000199:Actins; D024042:Collagen Type I; D024061:Collagen Type III; C486767:Il27 protein, mouse; C484747:Il27ra protein, mouse; D007378:Interleukins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D053773:Transforming Growth Factor beta1; C544013:alpha-smooth muscle actin, mouse