title: Comparison of various semen extenders and addition of prostaglandin F2α on pregnancy rate in cows.
abstract: This investigation comprises three trials. Trial 1 consists of an in vitro comparison of three semen extenders: two egg yolk based (customized Tris-egg yolk-glycerol and Triladyl®), the third (AndroMed®) soybean lecithin based. With regard to post-thaw motility, the phytoextender AndroMed® proved to be superior (59±3% v. 53±2% and 53±2%, P<0.05). It had earlier been shown that addition of the commercial prostaglandin F2α preparation Dinolytic® before freezing compromises post-thaw motility; therefore, in Trial 2, Dinolytic® was added after thawing. Frozen-thawed spermatozoa tolerated addition of Dinolytic® at a concentration of 30% (v/v). In Trial 3, cows were inseminated using straws in which diluted semen and Dinolytic® were frozen in the same straw, separated by an air bubble, so intermingling could only take place in the course of insemination. Pregnancy rates at Dinolytic® dosages of 0%, 30% or 60% amounted to 44%, 41% and 56%, respectively (P>0.05), a result that encourages a large-scale field study, which is envisioned.
authors.affiliation: 1Department of Animal Science,Georg-August-University Goettingen,Albrecht-Thaer-Weg 3,37075 Goettingen,Germany.
authors.forename: A M
authors.initials: AM
authors.lastname: Abdussamad
authors.affiliation: 2Verein Ostfriesischer Stammviehzuechter,AI and ET Center,26770 Georgsheil,Germany.
authors.forename: J
authors.initials: J
authors.lastname: Detterer
authors.affiliation: 1Department of Animal Science,Georg-August-University Goettingen,Albrecht-Thaer-Weg 3,37075 Goettingen,Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gauly
authors.affiliation: 1Department of Animal Science,Georg-August-University Goettingen,Albrecht-Thaer-Weg 3,37075 Goettingen,Germany.
authors.forename: W
authors.initials: W
authors.lastname: Holtz
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D015237:Dinoprost / Q000008:administration & dosage / Q000494:pharmacology*; D004530:Egg Yolk; D005260:Female; D005615:Freezing; D005990:Glycerol; D007315:Insemination, Artificial / Q000662:veterinary*; D007552:Isotonic Solutions; D054709:Lecithins; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen / Q000737:chemistry* / Q000502:physiology; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D007552:Isotonic Solutions; D054709:Lecithins; C503302:triladyl; D015237:Dinoprost; D005990:Glycerol
keywords: artificial insemination; cattle; prostaglandin F2α; semen extender; spermatozoan motility