title: Impact of nicotine on the interplay between human periodontal ligament cells and CD4+ T cells.
abstract: Periodontitis is a common infectious disease associated with destruction of periodontal ligaments and alveolar bones. CD4(+) T cell-mediated immune response is involved in the progression of periodontitis. Tobacco consumption increases the risk of periodontal disease. However, the impact of nicotine on the interaction between human periodontal ligament (PDL) cells and CD4(+) T cells remains unrevealed. Our study aims to investigate the effect of nicotine on PDL cells and the cocultured CD4(+) T cells. The PDL cell cultures were established by explants from healthy individuals, exposed to nicotine or α-bungarotoxin (α-BTX), and incubated solely or in combination with CD4(+) T cells. Afterwards, cell viability, secreted cytokines, and matrix metalloproteinases (MMPs) were evaluated. In monoculture of PDL cells, nicotine dramatically repressed cell viability and increased apoptosis. Meanwhile, α-BTX largely reversed the nicotine-induced apoptosis and increased viability of PDL cells. Compared with the monoculture, MMP-1, MMP-3, interleukin (IL)-1β, IL-6, IL-17, and IL-21 in supernatant of cocultures were markedly elevated after treatment with nicotine. Moreover, α-BTX significantly attenuated nicotine-triggered production of these components either in mono- or co-cultures. In addition, PDL cell-derived CXCL12 following nicotine treatment recruited CD4(+) T cells. Above all, nicotine deteriorated periodontitis partially by promoting PDL cell-CD4(+) T cell-mediated inflammatory response and matrix degradation.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Stomatology, The 464 Hospital of Beijing PLA, Tianjin, China.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wong
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Li-Zheng
authors.initials: LZ
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China wxjingpd@hotmail.com.
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002038:Bungarotoxins / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D002450:Cell Communication / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009538:Nicotine / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D010518:Periodontitis / Q000139:chemically induced / Q000276:immunology
chemical_list: D002038:Bungarotoxins; D016207:Cytokines; D009538:Nicotine; D020782:Matrix Metalloproteinases
keywords: CD4+ T cells; Nicotine; human periodontal ligament cells; inflammation; periodontitis; α-bungarotoxin