title: Multiplexed MALDI-MS arrays for screening of MIP solid phase extraction materials.
abstract: Technology that facilitates rapid investigation of solid phase extraction protocols using very small amounts of sorbent can save both time and money. The microfabricated ISET (Integrated Selective Enrichment Target) interfaced with MALDI mass spectrometry is able to provide an efficient, economic and generic optimization process for SPE sample preparation. The SPE is performed in a rapid and parallel fashion, with a processing time off only 2h per ISET with 96 samples. Each of the 96 wells on the ISET can hold 600nL of SPE sorbent. The ability to work with small amounts of sorbent and samples in the ISET platform provides a big advantage when developing affinity sorbents, such as molecularly imprinted polymers (MIPs). Here it is demonstrated that an amount of 25mg phosphoserine imprinted MIP (pS-MIP) sorbent can allow for analysis of more than 500 ISET nanovials using a multitude of different conditions. In the presented case, the multiplexed experiments allowed for early discovery of unspecific interactions and subsequent minimization of these, resulting in a protocol that provided improved enrichment of phosphopeptides.
authors.affiliation: Department of Biomedical Engineering, Lund University, Lund, Sweden. Electronic address: kishore.jagadeesan@bme.lth.se.
authors.forename: Kishore Kumar
authors.initials: KK
authors.lastname: Jagadeesan
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Sweden.
authors.forename: Celina
authors.initials: C
authors.lastname: Wierzbicka
authors.affiliation: Department of Biomedical Engineering, Lund University, Lund, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Sweden.
authors.forename: Börje
authors.initials: B
authors.lastname: Sellergren
authors.affiliation: Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Sweden.
authors.forename: Sudhirkumar
authors.initials: S
authors.lastname: Shinde
authors.affiliation: Department of Biomedical Engineering, Lund University, Lund, Sweden.
authors.forename: Simon
authors.initials: S
authors.lastname: Ekström
mesh_terms: D000818:Animals; D002364:Caseins; D002417:Cattle; D010768:Phosphoserine; D040901:Proteomics / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002364:Caseins; D010768:Phosphoserine
keywords: ISET; MALDI MS; Mass spectrometry; Proteomics; Solid-phase extraction