title: Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator.
abstract: Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified prominent Histone H3 acetylation activity in vitro and purified its activity, showing that it is composed of the MYST acetyltransferase Chameau and Enhancer of the Acetyltransferase Chameau (EAChm) family. EAChm is a negatively charged acidic protein retaining aspartate and glutamate. Furthermore, we identified that Chameau and EAChm stimulate transcription in vitro together with purified general transcription factors. In addition, RNA-seq analysis of Chameu KD and EAChm KD S2 cells suggest that Chameau and EAChm regulate transcription of common genes in vivo. Our results suggest that EAChm regulates gene transcription in Drosophila embryos by enhancing Acetyltransferase Chameau activity.
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Takeya
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ikehara
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Doiguchi
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Imamura
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Higashi
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yoneda
authors.affiliation: Department of Biochemistry, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D015534:Trans-Activators / Q000737:chemistry / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D015534:Trans-Activators; D000123:Acetyltransferases; C467710:chm protein, Drosophila