title: Structural characteristics of pectic polysaccharides and arabinogalactan proteins from Heracleum sosnowskyi Manden.
abstract: Polymers with different structures were isolated from the aboveground part of Heracleum sosnowskyi Manden. The sequential treatment of Heracleum with water, HCl solution, and (NH4)2C2O4 solution was observed to decrease the arabinogalactan proteins (AGP) content and increase the pectins content in the extracted polysaccharides. The linear region of the HSO-I polysaccharide having the highest yield was found to be composed mainly of partially methylesterified homogalacturonan fragments, whereas the branched region was made up of fragments of rhamnogalacturonan I whose core represents 1,2-α-L-rhamno-1,4-α-D-galacturonan. The carbohydrate side chains of the branched region are linked to the α-L-Rhap core residues via the 1,4-glycosidic bond and consist chiefly of T-β-D-Galp, 1,4-β-D-Galp and 1,6-β-D-Galp residues indicating the presence of the 1,4-β-D-galactan. NMR spectroscopy revealed the carbohydrate moiety of the AGP molecule to consist mainly of 1,3- and 1,3,6-β-D-Galp residues. The side chains comprised 1,6-β-D-Galp, terminal 4-O-Me-β-D-GlcpA, and β-D-Galp.
authors.affiliation: Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
authors.forename: Evgeny G
authors.initials: EG
authors.lastname: Shakhmatov
authors.affiliation: Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), Biysk 659322, Russia.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Atukmaev
authors.affiliation: Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia. Electronic address: makarowa.elena-ma@yandex.ru.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Makarova
mesh_terms: D002240:Carbohydrate Sequence; D030002:Heracleum / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000737:chemistry* / Q000302:isolation & purification; D019815:Oxalic Acid / Q000737:chemistry; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification; D035261:Plant Components, Aerial / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
chemical_list: D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; D014867:Water; D010368:Pectins; D019815:Oxalic Acid; D006851:Hydrochloric Acid
keywords: Arabinogalactan protein; Heracleum sosnowskyi; NMR; Pectin; Structure