title: [Effects of interleukin-18 and hypoxia-inducible factor-1α in serum and gingival tissues of rat model with periodontitis exposed to chronic intermittent hypoxia].
abstract: OBJECTIVE
  This study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-lα in rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods: Thirty-two Sprague-Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-lα in hypoxia periodontitis group was evaluated.
  
  
  RESULTS
  The levels of IL-18 and HIF-lα in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-lα in serum (r-0.792, r=0.753, P<0.05) and gingival tissues (r-0.817, r=0.779, P<0.05) were positively correlated with AL.
  
  
  CONCLUSIONS
  CIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-lα levels.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005881:Gingiva; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020382:Interleukin-18; D010512:Periodontal Index; D010518:Periodontitis; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D020181:Sleep Apnea, Obstructive
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020382:Interleukin-18