title: The Isolation, Identification and Analyses of Lactobacillus Genus Bacteria with Probiotic Potential.
abstract: 103 strains of lactic acid bacteria of Lactobacillus genus were isolated from natural sources and identified for genus and species level with API tests and 16S rRNA sequencing. However, only 27 strains from isolated cultures demonstrated a high stability to gastric stress and from that - only 15 strains were highly resistant to intestinal stress. Results indicated that only some isolated cultures of lactobacilli possessed potential probiotical properties and could serve as new probiotics for dairy industry with high resistance to gastro-intestinal stresses.
authors.affiliation: Microbiology Department, Moscow Lomonosov State University, Moscow, 119992, Russia.
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Cherdyntseva
authors.affiliation: Microbiology Department, Moscow Lomonosov State University, Moscow, 119992, Russia.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Kotova
authors.affiliation: Microbiology Department, Moscow Lomonosov State University, Moscow, 119992, Russia. anetrusov@mail.ru.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Netrusov
mesh_terms: D000818:Animals; D003612:Dairying; D005516:Food Microbiology; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D019936:Probiotics / Q000145:classification / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013312:Stress, Physiological
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Gastro-intestinal stress; Isolation of lactic acid bacteria; Molecular identification; Probiotic properties