title: Carbonic anhydrases and their functional differences in human and mouse sperm physiology.
abstract: Fertilization is a key reproductive event in which sperm and egg fuse to generate a new individual. Proper regulation of certain parameters (such as intracellular pH) is crucial for this process. Carbonic anhydrases (CAs) are among the molecular entities that control intracellular pH dynamics in most cells. Unfortunately, little is known about the function of CAs in mammalian sperm physiology. For this reason, we re-explored the expression of CAI, II, IV and XIII in human and mouse sperm. We also measured the level of CA activity, determined by mass spectrometry, and found that it is similar in non-capacitated and capacitated mouse sperm. Importantly, we found that CAII activity accounts for half of the total CA activity in capacitated mouse sperm. Using the general CA inhibitor ethoxyzolamide, we studied how CAs participate in fundamental sperm physiological processes such as motility and acrosome reaction in both species. We found that capacitated human sperm depend strongly on CA activity to support normal motility, while capacitated mouse sperm do not. Finally, we found that CA inhibition increases the acrosome reaction in capacitated human sperm, but not in capacitated mouse sperm.
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico.
authors.forename: O
authors.initials: O
authors.lastname: José
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico.
authors.forename: P
authors.initials: P
authors.lastname: Torres-Rodríguez
authors.affiliation: Abteilung Allgemeine Zoologie, Fachbereich Biologie, University of Kaiserslautern, D-67653, Kaiserslautern, Germany.
authors.forename: L S
authors.initials: LS
authors.lastname: Forero-Quintero
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico.
authors.forename: J C
authors.initials: JC
authors.lastname: Chávez
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico.
authors.forename: J L
authors.initials: JL
authors.lastname: De la Vega-Beltrán
authors.affiliation: Dipartimento di Chimica "U. Schiff", Laboratorio di Chimica Bioinorganica, Universitá degli Studi di Firenze, Via della Lastruccia 3, Polo Scientifico, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carta
authors.affiliation: Dipartimento di Chimica "U. Schiff", Laboratorio di Chimica Bioinorganica, Universitá degli Studi di Firenze, Via della Lastruccia 3, Polo Scientifico, 50019, Sesto Fiorentino, Florence, Italy; Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche, Universitá degli Studi di Firenze, Via Ugo Schiff 6, Polo Scientifico, 50019, Sesto Fiorentino, Florence, Italy.
authors.forename: C T
authors.initials: CT
authors.lastname: Supuran
authors.affiliation: Abteilung Allgemeine Zoologie, Fachbereich Biologie, University of Kaiserslautern, D-67653, Kaiserslautern, Germany.
authors.forename: J W
authors.initials: JW
authors.lastname: Deitmer
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Morelos, Mexico. Electronic address: ctrevino@ibt.unam.mx.
authors.forename: C L
authors.initials: CL
authors.lastname: Treviño
mesh_terms: D000177:Acrosome / Q000201:enzymology*; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D002256:Carbonic Anhydrases
keywords: Acrosome reaction; Carbonic anhydrase; Motility; Sperm