title: Blueberry anthocyanins ameliorate radiation-induced lung injury through the protein kinase RNA-activated pathway.
abstract: The purpose of this study was to explore the effect of blueberry anthocyanins (BA) on radiation-induced lung injury and investigate the mechanism of action. Seven days after BA(20 and 80 mg/kg/d)administration, 6 weeks old male Sprague-Dawley rats rats were irradiated by LEKTA precise linear accelerator at a single dose of 20 Gy only once. and the rats were continuously treated with BA for 4 weeks. Moreover, human pulmonary alveolar epithelial cells (HPAEpiC) were transfected with either control-siRNA or siRNA targeting protein kinase R (PKR). Cells were then irradiated and treated with 75 μg/mL BA for 72 h. The results showed that BA significantly ameliorated radiation-induced lung inflammation, lung collagen deposition, apoptosis and PKR expression and activation. In vitro, BA significantly protected cells from radiation-induced cell death through modulating expression of Bcl-2, Bax and Caspase-3. Suppression of PKR by siRNA resulted in ablation of BA protection on radiation-induced cell death and modulation of anti-apoptotic and pro-apoptotic proteins, as well as Caspase-3 expression. These findings suggest that BA is effective in ameliorating radiation-induced lung injury, likely through the PKR signaling pathway.
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China.
authors.forename: Yunen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Dehong
authors.initials: D
authors.lastname: Tan
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China.
authors.forename: Changci
authors.initials: C
authors.lastname: Tong
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China.
authors.forename: Yubiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, The General Hospital of Shenyang Military Command, Shenyang 110016, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang 110016, China. Electronic address: houmingxiao188@163.com.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Hou
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D029798:Blueberry Plants / Q000737:chemistry*; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D053148:Caspase 3 / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003094:Collagen / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D055370:Lung Injury / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects / Q000528:radiation effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011833:Radiation Injuries, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects* / Q000528:radiation effects; D051028:bcl-2-Associated X Protein / Q000235:genetics; D019892:eIF-2 Kinase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D003094:Collagen; D019892:eIF-2 Kinase; D053148:Caspase 3
keywords: Blueberry anthocyanins; Lung injury; Natural pigment; PKR; Radiation