title: Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.
abstract: The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random ampliﬁed polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. meizhiqiang@scmu.edu.cn.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. 20140199120007@stu.scmu.edu.cn.
authors.forename: Boxu
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. weichunli2015@scmu.edu.cn.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China. weichunli2015@scmu.edu.cn.
authors.forename: Chunli
authors.initials: C
authors.lastname: Wei
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. jingliangc@scmu.edu.cn.
authors.forename: Jingliang
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. saberimani@scmu.edu.cn.|Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran 14359-44711, Iran. saberimani@scmu.edu.cn.
authors.forename: Saber
authors.initials: S
authors.lastname: Imani
authors.affiliation: Department of Biochemistry, School of Life Sciences & the State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, China. chenhanchun@csu.edu.cn.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China. fujunjiang@lzmc.edu.cn.|State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China. fujunjiang@lzmc.edu.cn.|Judicial Authentication Center, Sichuan Medical University, Luzhou 646000, China. fujunjiang@lzmc.edu.cn.
authors.forename: Junjiang
authors.initials: J
authors.lastname: Fu
mesh_terms: D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018744:DNA, Plant; D032062:Gardenia / Q000145:classification* / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: G. jasminoides Ellis var. grandiflora Nakai; Gardenia jasminoides; cloning; genetic authentication; random ampliﬁed polymorphic DNA; sequence-characterized amplified region