title: Gene expression profile of vascular ischemia-reperfusion injury in rhesus monkeys.
abstract: The vascular system particularly endothelium is sensitive to ischemia-reperfusion (I/R) injury, which is a big challenge in surgical practices and many vascular disorders. In the present study, we reported the global gene expression changes in a 2-h ischemia and 4-h reperfusion injury induced in the hind limb vessels of rhesus monkeys (Macaca mulatta) using microarray technique.
  
  
  RESULTS
  The histological results showed abnormal morphology of endothelial cells after 2-h ischemia and the hematological detection found slightly extension of coagulation time after I/R treatment. Furthermore, we found distinct alterations in gene expression patterns during I/R process. These identified genes are mostly involved in inflammation, immune response, apoptosis, and cell stress signaling pathways. The significantly up-regulated genes included IL-6, regulator of G-protein signaling 8, selectin E, and metallothionein 2A, et al. Whist, the robustly down-regulated genes included NECAP endocytosis associated 2, transglutaminase 2, and fibronectin 1, et al.
  
  
  CONCLUSIONS
  Our results indicate that inflammation, primarily characterized by gene expression changes of cytokines and chemokines is the most important event in the early stage of I/R injury in blood vessels.
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Ruixi
authors.initials: R
authors.lastname: Luo
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Vascular Surgery, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Xiaojiong
authors.initials: X
authors.lastname: Du
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Meimei
authors.initials: M
authors.lastname: Shi
authors.affiliation: Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Chunguang
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Chengshi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Animal Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Guangneng
authors.initials: G
authors.lastname: Liao
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: National Center for Safety Evaluation of Traditional Chinese Medicine, Chengdu, Sichuan, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China. Electronic address: jqcheng@scu.edu.cn.
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Transplant Engineering and Immunology, NHFPC; Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China. Electronic address: younanchen@126.com.
authors.forename: Younan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology*; D016000:Cluster Analysis; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D007511:Ischemia / Q000097:blood / Q000235:genetics*; D008253:Macaca mulatta; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000097:blood / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
keywords: Blood vessel; Gene expression; Ischemia and reperfusion; Rhesus monkey