title: A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors.
abstract: A bio-acoustic levitational assembly method for engineering of multilayered, 3D brainlike constructs is presented. Acoustic radiation forces are used to levitate neuroprogenitors derived from human embryonic stem cells in 3D multilayered fibrin tissue constructs. The neuro-progenitor cells are subsequently differentiated in neural cells, resulting in a 3D neuronal construct with inter and intralayer neurite elongations.
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.|Inserm, U1032, LabTau, University of Lyon, Lyon, F-69003, France.|LabEx DEVweCAN, University of Lyon, Lyon, F-69003, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Bouyer
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Pu
authors.initials: P
authors.lastname: Chen
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Sinan
authors.initials: S
authors.lastname: Güven
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Tuğrul Tolga
authors.initials: TT
authors.lastname: Demirtaş
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
authors.forename: Thomas J F
authors.initials: TJ
authors.lastname: Nieland
authors.affiliation: Inserm, U1032, LabTau, University of Lyon, Lyon, F-69003, France.|LabEx DEVweCAN, University of Lyon, Lyon, F-69003, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Padilla
authors.affiliation: Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center for Early Cancer Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.|Department of Electrical Engineering (By Courtesy), Stanford University, Stanford, CA, 94305, USA.
authors.forename: Utkan
authors.initials: U
authors.lastname: Demirci
mesh_terms: D000162:Acoustics; D001921:Brain / Q000166:cytology*; D002454:Cell Differentiation; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
keywords: bulk acoustic standing waves; multilayered 3D tissue structures; neural constructs; neural progenitor cells; tissue engineering