title: In Vitro Seeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae)).
abstract: Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficient in vitro regeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three types of seeds were inoculated on different basal media devoid of growth regulators. Various strengths of the medium of choice and the type and concentration of carbon source were also investigated. Responses to germination varied with the type of seed. Embryonic axis (EA) followed by seeds without coat (SWtC) germinated rapidly and expressed a high rate of germination. The growth performances of plantlets varied with the basal medium composition and the seeds type. The optimal growth performances of plants were displayed on half strength MS basal medium with SWtC and EA as source of seeds. Addition of 3% sucrose in the culture medium was more suitable for a maximum growth of plantlets derived from EA.
authors.affiliation: Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire.
authors.forename: Mongomaké
authors.initials: M
authors.lastname: Koné
authors.affiliation: Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire.
authors.forename: Tchoa
authors.initials: T
authors.lastname: Koné
authors.affiliation: Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire ; Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët-Boigny, 22 BP 582, Abidjan 22, Côte d'Ivoire.
authors.forename: Nakpalo
authors.initials: N
authors.lastname: Silué
authors.affiliation: Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire.
authors.forename: André Brahima
authors.initials: AB
authors.lastname: Soumahoro
authors.affiliation: Laboratoire de Biologie et Amélioration des Productions Végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801, Abidjan 02, Côte d'Ivoire.
authors.forename: Tanoh Hilaire
authors.initials: TH
authors.lastname: Kouakou
mesh_terms: D050260:Carbohydrate Metabolism; D003470:Culture Media; D007887:Fabaceae / Q000254:growth & development* / Q000378:metabolism; D018525:Germination; D066298:In Vitro Techniques; D036226:Seedlings / Q000254:growth & development* / Q000378:metabolism; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
chemical_list: D003470:Culture Media; D013395:Sucrose