title: Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells.
abstract: The adoptive transfer of the natural regulatory B cells and macrophages should be a useful treatment for inflammation and autoimmune disease. However, it is usually difficult to isolate these cells from the tissues and expand them. Here, we investigated the feasibility of adoptively transferring peritoneal cells (PCs) as a treatment for DSS-induced colitis. We found that peritoneal cavity can provide an easily accessible site for harvesting enough number of PCs, namely, two-dose PCs for the treatment from a mouse in one operation. Adoptive therapy of these cells from healthy mice or those with disease is effectively in reducing the disease activity score. The natural B cells and macrophages of the infused PCs can selectively migrate to lesion sites and regulate the expression of Stat3, NF-κB, Smad3 and Smad7. Additionally, PCs exert dual activity of IL-10 and TGF-β secreted spontaneously by both peritoneal B cells and macrophages, which in turn enhance the induction of regulatory B cells and Macrophages in microenvironment of inflammation. Moreover, PCs can re-establish immunological tolerance in the OVA-immunized mice. Thus, our findings provide a new strategy for colitis therapy and could be of importance in additional exploration of other inflammation and autoimmune diseases therapy.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Xia-wei
authors.initials: XW
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Guo-bo
authors.initials: GB
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Yan-tong
authors.initials: YT
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Guang-chao
authors.initials: GC
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shao
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Sen-yi
authors.initials: SY
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Zhi-yao
authors.initials: ZY
authors.lastname: He
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Yan-Zhu
authors.initials: YZ
authors.lastname: Wen
authors.affiliation: Department of Immunology, Medical School of Nankai University, 94 Weijin Road, Tianjin 300071, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Hong-xin
authors.initials: HX
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center/National Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
authors.forename: Yu-quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003092:Colitis / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D016264:Dextran Sulfate / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000628:therapy; D016753:Interleukin-10 / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology; D051896:Smad Proteins, Receptor-Regulated / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: C508609:IL10 protein, human; D016328:NF-kappa B; D051896:Smad Proteins, Receptor-Regulated; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D016264:Dextran Sulfate