title: [Topography and mechanical property of goat temporomandibular joint disc cells].
abstract: OBJECTIVE
  This study is performed to investigate the cell topographies and biomechanical properties of two different types of temporomandibular joint (TMJ) discs from goats by using JPK Nano Wizard 3 biological atomic force microscopy (AFM). This process provides a guideline for selecting seed cells for TMJ disc tissue engineering.
  
  
  METHODS
  TMJ disc cells from primary goats were cultured by monolayer culture method. AFM was used to contact scan the topographies of the two types of TMJ disc cells under physiological environment. Approximately 20 chondrocyte-like and fibroblast-like cells were selected randomly to plot the force-versus-distance curves of the cytoplasm and nucleus. Young's modulus and adhesion were analyzed by JPK Data Processing.
  
  
  RESULTS
  The triangle-shapednucleus of the chondrocyte-like cell occupied a large portion of the cell. Cytoskeleton was arranged dendritically on the surface. Pseudopodia were extended from cell edges. The spindle-shaped nucleus of the fibroblast-like cell occupied a significantly larger region compared with the cytoplasmic region. Cytoskeleton was arranged regularly. Cell edges were smooth with less pseudopodia extended. No difference was found in the surface roughness between the two types of cells. According to the force-versus-distance curves, the Young's moduli of the two types of cells were not statistically different (P>0.05), but differences were found in the cytoplasmic regions (P=0.047). No statistical difference was found in the adhesions between the two types of cells (P>0.05).
  
  
  CONCLUSIONS
  The AFM topography and curves were compared and analyzed. The two types of TMJ disc cells exhibited significantly different topographies, but only slight difference in their mechanical abilities.
authors.forename: Guangjie
authors.initials: G
authors.lastname: Bao
authors.forename: Nannan
authors.initials: N
authors.lastname: Kong
authors.forename: Manli
authors.initials: M
authors.lastname: Guo
authors.forename: Xuelian
authors.initials: X
authors.lastname: Su
authors.forename: Hong
authors.initials: H
authors.lastname: Kang
mesh_terms: D000818:Animals; D019902:Chondrocytes; D055119:Elastic Modulus; D005347:Fibroblasts; D006041:Goats; D019224:Temporomandibular Joint Disc; D023822:Tissue Engineering