title: Hybrid chitosan-ß-glycerol phosphate-gelatin nano-/micro fibrous scaffolds with suitable mechanical and biological properties for tissue engineering.
abstract: Scaffold-based tissue engineering is considered as a promising approach in the regenerative medicine. Graft instability of collagen, by causing poor mechanical properties and rapid degradation, and their hard handling remains major challenges to be addressed. In this research, a composite structured nano-/microfibrous scaffold, made from a mixture of chitosan-ß-glycerol phosphate-gelatin (chitosan-GP-gelatin) using a standard electrospinning set-up was developed. Gelatin-acid acetic and chitosan ß-glycerol phosphate-HCL solutions were prepared at ratios of 30/70, 50/50, 70/30 (w/w) and their mechanical and biological properties were engineered. Furthermore, the pore structure of the fabricated nanofibrous scaffolds was investigated and predicted using a theoretical model. Higher gelatin concentrations in the polymer blend resulted in significant increase in mean pore size and its distribution. Interaction between the scaffold and the contained cells was also monitored and compared in the test and control groups. Scaffolds with higher chitosan concentrations showed higher rate of cell attachment with better proliferation property, compared with gelatin-only scaffolds. The fabricated scaffolds, unlike many other natural polymers, also exhibit non-toxic and biodegradable properties in the grafted tissues. In conclusion, the data clearly showed that the fabricated biomaterial is a biologically compatible scaffold with potential to serve as a proper platform for retaining the cultured cells for further application in cell-based tissue engineering, especially in wound healing practices. These results suggested the potential of using mesoporous composite chitosan-GP-gelatin fibrous scaffolds for engineering three-dimensional tissues with different inherent cell characteristics.
authors.affiliation: Department of Modern Sciences & Technologies School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Lotfi
authors.affiliation: Advanced Textile Materials and Technology Research Institute (ATMT), Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Roohollah
authors.initials: R
authors.lastname: Bagherzadeh
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
authors.forename: Hojjat
authors.initials: H
authors.lastname: Naderi-Meshkin
authors.affiliation: Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Mahdipour
authors.affiliation: Pathology Department of Shahid Kamyab (Emdadi) Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Mafinezhad
authors.affiliation: Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Sadeghnia
authors.affiliation: Department of Biostatistics and Epidemiology, School of Health, Mashhad University of Medical Sciences Mashhad, Iran.
authors.forename: Habibollah
authors.initials: H
authors.lastname: Esmaily
authors.affiliation: Cutaneous Leishmaniasis Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Maleki
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
authors.forename: Halimeh
authors.initials: H
authors.lastname: Hasssanzadeh
authors.affiliation: Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medicine, Mashhad, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayaour-Mobarhan
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Bidkhori
authors.affiliation: Stem Cell and Regenerative Medicine Research Group, Iranian Academic Center for Education, Culture and Research (ACECR), Khorasan Razavi Branch, Mashhad, Iran.|Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Bahrami
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D005994:Glycerophosphates / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D005994:Glycerophosphates; D005780:Gelatin; D048271:Chitosan; C031463:beta-glycerophosphoric acid
keywords: biocompatible scaffold; chitosan; gelatin; regenerative medicine; tissue engineering