title: Lipofuscin Granules in the Epileptic Human Temporal Neocortex with Age.
abstract: Lipofuscin granules (LGs), the "age pigments", are autofluorescent cell products from lysosomes that diverge in number and size among brain regions. Human temporal cortex from 20- to 55-year-old epileptic subjects were studied with the fat soluble dye Sudan Black, under confocal and electron microscopy. Ultrastructural analysis showed that with age LGs increase in area, but not in number. Proportionally to the LGs area, the electron lucid portion increases and the electron dense reduces over time. The robust increase in lipid components is possibly due to modifications in the neuronal metabolism with age in physiological and pathological conditions.
authors.affiliation: a Department of Neuroscience and Behavior .
authors.forename: Suélen
authors.initials: S
authors.lastname: Merlo
authors.affiliation: a Department of Neuroscience and Behavior .
authors.forename: Ana Beatriz S
authors.initials: AB
authors.lastname: Nakayama
authors.affiliation: a Department of Neuroscience and Behavior .
authors.forename: Janaina
authors.initials: J
authors.lastname: Brusco
authors.affiliation: b Department of Pathology and Forensic Medicine , and.
authors.forename: Marcos A
authors.initials: MA
authors.lastname: Rossi
authors.affiliation: c Department of Surgery and Anatomy , Ribeirão Preto School of Medicine, University of São Paulo , Ribeirão Preto, SP , Brazil.
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Carlotti
authors.affiliation: a Department of Neuroscience and Behavior .|b Department of Pathology and Forensic Medicine , and.
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Moreira
mesh_terms: D000328:Adult; D000375:Aging / Q000473:pathology*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004833:Epilepsy, Temporal Lobe / Q000473:pathology; D005260:Female; D006801:Humans; D008062:Lipofuscin / Q000032:analysis*; D008297:Male; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D019579:Neocortex / Q000648:ultrastructure; D009474:Neurons / Q000648:ultrastructure*; D013702:Temporal Lobe / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D008062:Lipofuscin
keywords: Aging; Sudan Black staining; confocal microscopy; epileptic human temporal neocortex; lipofuscin granules; transmission electron microscopy