title: Enzymatic properties of chitinase-producing antagonistic bacterium Paenibacillus chitinolyticus with various substrates.
abstract: Various chitin substrates were used to investigate the properties of enzymes produced from the chitinase-producing bacterium Paenibacillus chitinolyticus MP-306 against phytopathogens. The MP-306 bacterium was incubated in nine culture media [crab shell powder chitin (CRS), chitin-protein complex powder (CPC), carboxymethyl-chitin powder (CMC), yeast extract only (YE), LB (Trypton, NaCl, and yeast extract), GT (Trypton, NaCl, and glucose), crab shell colloidal chitin (CSC), squid pen powder chitin (SPC), and cicada slough powder chitin (CSP)] at 30 °C for 3 days. Chitinase isozymes in CPC medium were expressed strongly as CN1, CN2, CN3, CN4, CN5, and CN6 bands on native-PAGE gels. Chitinase isozymes in CPC and CMC medium were expressed as 13 bands (CS1-CS13) on SDS-PAGE gels. Chitinase isozymes were expressed strongly on SDS-PAGE gels as two bands (CS6 and CS8) on YE and LB medium and 13 bands (CS1-CS13) on SPC medium. In crude enzyme, chitinase isozymes at pH 7 and pH 9 in chitin media appeared strongly on SDS-PAGE gels. Partial purified enzyme indicated high stability of enzyme activity at various temperatures and pHs in chitin medium, while these enzymes indicated low activity staining of enzyme on electrophoresis gels at various temperatures and pHs condition of chitin medium.
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Song
authors.affiliation: Fermented Food Science Division, Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Jeollabuk-do 565-851, South Korea.
authors.forename: Dong-Jun
authors.initials: DJ
authors.lastname: Seo
authors.affiliation: Sericulture and Apiculture Division, Department of Agriculutral Biology, National Academy of Agricultural Science, Rural Development Administration, Jeollabuk-do 565-851, South Korea.
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Ju
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Yong-Seong
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, South Korea. Electronic address: woojung@chonnam.ac.kr.
authors.forename: Woo-Jin
authors.initials: WJ
authors.lastname: Jung
mesh_terms: D002686:Chitin / Q000378:metabolism*; D002688:Chitinases / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D056507:Paenibacillus / Q000201:enzymology* / Q000254:growth & development*; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D003470:Culture Media; D002686:Chitin; D012965:Sodium Chloride; D002688:Chitinases
keywords: Chitin; Chitinase isozymes; Cicada slough; Crab shell; Paenibacillus chitinolyticus; Squid pen