title: [Improvement of natamycin production in an industrial strain by heterologous expression of the afsRS(cla) global regulatory genes].
abstract: The afsRS(cla) global regulatory genes from Streptomyces clavuligerus activate the production of two antibiotics in Streptomyces lividans. In this study, we gained an increase of 38% in the production of natamycin (3.56 g/L) in an industrial strain Streptomyces gilvosporeus TZ1401 through the integration of pHL851 that bears the afsRS(cla) global regulatory genes into its genome. We discovered by quantitive real-time reverse transcription PCR (qRT-PCR) that the expression of 6 genes of the natamycin biosynthetic gene cluster were improved from 1.9 to 2.7 times. This suggests that afsRS(cla) improve the production of natamycin through increased transcription. This study provides a good example for applying afsRS(cla) in high yield breeding of industrial antibiotic producers.
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Tao
authors.forename: Yemin
authors.initials: Y
authors.lastname: Wang
authors.forename: Hualiang
authors.initials: H
authors.lastname: Zheng
authors.forename: Meifeng
authors.initials: M
authors.lastname: Tao
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D005809:Genes, Regulator; D007218:Industrial Microbiology; D005810:Multigene Family; D010866:Natamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D010866:Natamycin