title: Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells.
abstract: Variable angle total internal reflection fluorescence microscopy (VA-TIRFM) is an optical method to observe the molecular events occurring in an extremely thin region near the plasma membrane. Recently, the VA-TIRFM technique has been widely used to study fluorescently labeled target molecules in living animal and plant cells. Here, we describe the optical principle of the VA-TIRFM technique and provide a detailed experimental procedure for the study of living plant cells.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing, 100083, China.
authors.forename: Yinglang
authors.initials: Y
authors.lastname: Wan
authors.affiliation: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing, 100083, China.
authors.forename: Ruili
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing, 100083, China. linjinxing@bjfu.edu.cn.|Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. linjinxing@bjfu.edu.cn.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
mesh_terms: D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: D010940:Plant Proteins
keywords: In vivo; Intact; Plant cells; Total internal reflection; VA-TIRFM