title: Molecular Characterization of Endoplasmic Reticulum Oxidoreductin 1 from Bombyx mori.
abstract: We isolated a complementary DNA (cDNA) clone encoding endoplasmic reticulum oxidoreductin 1 (bERO1, a specific oxidant of protein disulfide isomerase (PDI)) from Bombyx mori. This protein has a putative open reading frame (ORF) of 489 amino acids and a predicted size of 57.4 kDa. Although bERO1 protein shares less than 57% amino acid sequence homology with other reported ERO1s, it contains two conserved redox active motifs, a Cys-X-X-X-X-Cys motif of N-terminal and Cys-X-X-Cys-X-X-Cys motif of C-terminal. Both motifs are typically present in ERO1 protein family members. The bEro1 mRNA expression was highest in posterior silk gland on the sixth day of the 5th instar larvae. Expression of bEro1 mRNA also markedly increased during endoplasmic reticulum (ER) stress induced by stimulation with antimycin, calcium ionophore A23187, dithiothreitol, H₂O₂, monencin, and tunicamycin. In addition, expression levels of bEro1 exactly coincided with that of bPdi. This is the first result suggesting that bERO1 plays an essential role in ER quality control through the combined activities of bERO1 and bPDI as a catalyst of protein folding in the ER and sustaining cellular redox homeostasis.
authors.affiliation: Institute of Medical Research, Dongguk University College of Medicine, Gyeongju 38067, Korea. nansmc@hanmail.net.
authors.forename: Minchul
authors.initials: M
authors.lastname: Seo
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 55365, Korea. autoomne@gmail.com.
authors.forename: Hee-Joo
authors.initials: HJ
authors.lastname: Ryou
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-gun 55365, Korea. yuney@korea.kr.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Yun
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 38067, Korea. gootw@dongguk.ac.kr.
authors.forename: Tae-Won
authors.initials: TW
authors.lastname: Goo
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs
chemical_list: D018076:DNA, Complementary; D019704:Protein Disulfide-Isomerases
keywords: Bombyx mori; disulfide bond; endoplasmic reticulum oxidoreductin 1 (ERO1); protein disulfide isomerase (PDI)