title: Adaptation of microcystin thiol derivatization for matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis.
abstract: Thiol derivatization of microcystins is an emerging technique for simplifying mass spectrometric analysis of microcystins in complex matrices and for distinguishing between Mdha-/Dhb-containing microcystins. The present protocol is not compatible with MALDI-TOF mass spectrometry, but use of sodium carbonate buffer and desalting with ZipTips yielded no loss in sensitivity. Use of ammonium carbonate buffer followed by dilution in MALDI matrix resulted in a small loss of sensitivity but allowed accurate determination of reaction rates.
authors.affiliation: Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand. Electronic address: jonathan.puddick@cawthron.org.nz.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Puddick
authors.affiliation: Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106 Oslo, Norway; Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway.
authors.forename: Christopher O
authors.initials: CO
authors.lastname: Miles
authors.affiliation: Department of Chemistry, University of Waikato, Private Bag 3105, Hamilton 3240, New Zealand.
authors.forename: Michèle R
authors.initials: MR
authors.lastname: Prinsep
mesh_terms: D052998:Microcystins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D052998:Microcystins; D013438:Sulfhydryl Compounds
keywords: MALDI-TOF mass spectrometry; Microcystin; Thiol derivatization