title: Immunosensing of Atrazine with Antibody-Functionalized Cu-MOF Conducting Thin Films.
abstract: This work reports the assembly of thin films of a silica (SiO2)-modified copper-metal organic framework, Cu3(BTC)2 [Cu3(BTC)2@SiO2, BTC = benzene-1,3,5-tricarboxylic acid] on a conducting substrate of NH2-BDC [NH2-BDC = 2-aminobenzene-1,4-dicarboxylic acid] doped polyaniline (PANI). Assembled Cu3(BTC)2@SiO2/BDC-PANI thin films displayed electrical conductivity in the range of 35 μA. These thin films were conjugated with antiatrazine antibodies to create a novel immunosensing platform. Various structural and spectral characteristics of the synthesized material and its bioconjugate were investigated. The developed immunosensor was used for the conductometric sensing of atrazine. The detection of atrazine was achieved with a high sensor sensitivity (limit of detection = 0.01 nM) and specificity in the presence of diverse pesticides (e.g., endosulfan, parathion, paraoxon, malathion, and monochrotophos).
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.|Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Bhardwaj
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.|Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Bhardwaj
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.|Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
authors.forename: Girish C
authors.initials: GC
authors.lastname: Mohanta
authors.affiliation: Department of Civil & Environmental Engineering, Hanyang University , 222 Wangsimni-Ro, Seoul 133-791, Republic of Korea.
authors.forename: Pawan
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.|Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
authors.forename: Amit L
authors.initials: AL
authors.lastname: Sharma
authors.affiliation: Department of Civil & Environmental Engineering, Hanyang University , 222 Wangsimni-Ro, Seoul 133-791, Republic of Korea.
authors.forename: Ki-Hyun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Central Scientific Instruments Organisation (CSIR-CSIO) , Sector 30 C Chandigarh 160030, India.|Academy of Scientific and Innovative Research, CSIR-CSIO , Sector 30 C, Chandigarh 160030, India.
authors.forename: Akash
authors.initials: A
authors.lastname: Deep
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000906:Antibodies / Q000378:metabolism*; D001280:Atrazine / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D004553:Electric Conductivity; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000814:Aniline Compounds; D000906:Antibodies; C416807:polyaniline; D003300:Copper; D001280:Atrazine
keywords: MOF; atrazine; conducting; polyaniline; sensor; thin films