title: Rare transmission of hepatitis B virus by Dutch donors with occult infection.
abstract: BACKGROUND
  To reduce the rate of transfusion-transmitted hepatitis B virus (HBV), HBV DNA testing was introduced for all Dutch blood donations in 2008, in addition to the existing screening for HBV surface antigen (HBsAg). This study describes the lookback results for repeat donors with an "HBV DNA-only" test result (HBV DNA-positive and HBsAg-negative).
  
  
  METHODS
  From November 2008 until June 2011, a total of 2.3 million blood donations were tested for HBV DNA and 22 donors showed the HBV DNA-only profile. Four donors had early preseroconversion HBV infection, two showed suppressed infection after vaccination, and 16 donors had occult chronic HBV infection (OBI). Potentially infectious donations were traced back as far as 1992. If possible the recipients were tested for current and past HBV infection.
  
  
  RESULTS
  A total of 416 implicated donations could be traced in blood bank records, involving 448 issued blood products. For 170 (38%) of the recipients no information was obtained from the hospitals; 196 (44%) recipients had died, and 82 (18%) were tested for HBV. Six recipients tested positive for current (n = 4) or past (n = 2) HBV infection. For two recipients transfusion was ruled out as the source of infection. Three patients showed HBV DNA sequences matching with the HBV in one common OBI donor. Overall, in four of 82 tested recipients (5%) HBV transmission was likely.
  
  
  CONCLUSIONS
  In our lookback study HBV testing was possible in only a minority (18%) of potentially exposed recipients. A low transmission rate (5%) was observed in recipients of blood components from donors with OBI.
authors.affiliation: Department of Blood-Borne Infections.
authors.forename: Ryanne W
authors.initials: RW
authors.lastname: Lieshout-Krikke
authors.affiliation: Department of Transfusion Medicine, Sanquin Blood Supply Foundation, Amsterdam, the Netherlands.
authors.forename: Marian G J
authors.initials: MG
authors.lastname: van Kraaij
authors.affiliation: Department of Transfusion Medicine, Sanquin Blood Supply Foundation, Amsterdam, the Netherlands.
authors.forename: Fikreta
authors.initials: F
authors.lastname: Danovic
authors.affiliation: Department of Blood-Borne Infections.
authors.forename: Hans L
authors.initials: HL
authors.lastname: Zaaijer
mesh_terms: D000328:Adult; D000368:Aged; D001771:Blood Banks / Q000706:statistics & numerical data; D001782:Blood Donors / Q000706:statistics & numerical data; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006509:Hepatitis B / Q000635:transmission*; D006515:Hepatitis B virus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D004279:DNA, Viral