title: miR-411 contributes the cell proliferation of lung cancer by targeting FOXO1.
abstract: Lung cancer is the leading cause of cancer deaths worldwide; the study of microRNAs gives new hope for lung cancer treatment. miR-411 has been demonstrated to be an independent prognostic factor for lung adenocarcinoma, but the role and regulatory mechanism are largely unknown. In the present study, we found miR-411 was overexpressed in the lung cancer cells; overexpression of miR-411 promoted anchorage-dependent and anchorage-independent growths of lung cancer, while miR-411 knockdown reduced this effect. Further study showed forkhead box O1 (FOXO1) was a target of miR-411. Overexpression of miR-411 suppressed the expression of FOXO1; the effect of suppression was abrogated when the mutation occurred in the 3'UTR of FOXO1. Knockdown of FOXO1 in cells which miR-411 was inhibited recapitulated the phenotype of miR-411 overexpression. Taken together, our study revealed miR-411 promoted cell proliferation of lung cancer by targeting tumor suppressor gene FOXO1 and miR-411 might be a potential target for lung cancer therapy.
authors.affiliation: Innovation Center for Cell Biology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230027, China. zzjzb@mail.ustc.edu.cn.|School of Life Sciences, University of Science and Technology of China, 443 Huangshan Rd., Hefei, Anhui, 230027, People's Republic of China. zzjzb@mail.ustc.edu.cn.
authors.forename: Zhiju
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Laboratory of Pathogen Biology, Center for Infection and Immunity, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Pathophysiology, Wannan Medical College, Wuhu, Anhui, 241002, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D000071161:Forkhead Box Protein O1 / Q000096:biosynthesis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; C582326:MIRN411 microRNA, human; D035683:MicroRNAs
keywords: Cell proliferation; FOXO1; Lung cancer; miR-411