title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass.
abstract: A high-density genetic map is essential for comparative genomic studies and fine mapping of QTL, and can also facilitate genome sequence assembly. Here, a high density genetic map of Asian seabass was constructed with 3321 SNPs generated by sequencing 144 individuals in a F2 family. The length of the map was 1577.67 cM with an average marker interval of 0.52 cM. A high level of genomic synteny among Asian seabass, European seabass, Nile tilapia and stickleback was detected. Using this map, one genome-wide significant and five suggestive QTL for growth traits were detected in six linkage groups (i.e. LG4, LG5, LG11, LG13, LG14 and LG15). These QTL explained 10.5-16.0% of phenotypic variance. A candidate gene, ACOX1 within the significant QTL on LG5 was identified. The gene was differentially expressed between fast- and slow-growing Asian seabass. The high-density SNP-based map provides an important tool for fine mapping QTL in molecular breeding and comparative genome analysis.
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Zi Yi
authors.initials: ZY
authors.lastname: Wan
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Bin
authors.initials: B
authors.lastname: Bai
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Shu Qing
authors.initials: SQ
authors.lastname: Huang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Elaine
authors.initials: E
authors.lastname: Chua
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: May
authors.initials: M
authors.lastname: Lee
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Hong Yan
authors.initials: HY
authors.lastname: Pang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Yan Fei
authors.initials: YF
authors.lastname: Wen
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Grace
authors.initials: G
authors.lastname: Lin
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
authors.forename: Bao Qing
authors.initials: BQ
authors.lastname: Ye
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.|Department of Biological Sciences, National University of Singapore, 14 Science Drive, Singapore 117543.|School of Biological Sciences, Nanyang Technological University, 6 Nanyang Drive, Singapore 637551.
authors.forename: Gen Hua
authors.initials: GH
authors.lastname: Yue
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics* / Q000254:growth & development*; D002874:Chromosome Mapping; D019295:Computational Biology / Q000379:methods; D005260:Female; D008040:Genetic Linkage; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D008126:Lod Score; D008297:Male; D058977:Molecular Sequence Annotation; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic