title: Revisiting the reference genomes of human pathogenic Cryptosporidium species: reannotation of C. parvum Iowa and a new C. hominis reference.
abstract: Cryptosporidium parvum and C. hominis are the most relevant species of this genus for human health. Both cause a self-limiting diarrhea in immunocompetent individuals, but cause potentially life-threatening disease in the immunocompromised. Despite the importance of these pathogens, only one reference genome of each has been analyzed and published. These two reference genomes were sequenced using automated capillary sequencing; as of yet, no next generation sequencing technology has been applied to improve their assemblies and annotations. For C. hominis, the main challenge that prevents a larger number of genomes to be sequenced is its resistance to axenic culture. In the present study, we employed next generation technology to analyse the genomic DNA and RNA to generate a new reference genome sequence of a C. hominis strain isolated directly from human stool and a new genome annotation of the C. parvum Iowa reference genome.
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia Carrera 53 No. 61-30, Medellin, Antioquia 05001, Colombia.|Centro Nacional de Secuenciación Genómica-CNSG, Universidad de Antioquia Carrera 53 No. 61-30, Medellin, Antioquia 05001, Colombia.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Isaza
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia Carrera 53 No. 61-30, Medellin, Antioquia 05001, Colombia.
authors.forename: Ana Luz
authors.initials: AL
authors.lastname: Galván
authors.affiliation: Universidad Mayor de Chile-Centro de Genómica y Bioinformatica Camino La piramide 5750 Huechuraba, Santiago de Chile, 8580000, Chile.
authors.forename: Victor
authors.initials: V
authors.lastname: Polanco
authors.affiliation: Virginia Commonwealth University - Center for the Study of Biological Complexity 1101 E. Marshall St., Virginia 23298-0678, US.
authors.forename: Bernice
authors.initials: B
authors.lastname: Huang
authors.affiliation: Virginia Commonwealth University - Center for the Study of Biological Complexity 1101 E. Marshall St., Virginia 23298-0678, US.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Matveyev
authors.affiliation: Virginia Commonwealth University - Center for the Study of Biological Complexity 1101 E. Marshall St., Virginia 23298-0678, US.
authors.forename: Myrna G
authors.initials: MG
authors.lastname: Serrano
authors.affiliation: Universidad Mayor de Chile-Centro de Genómica y Bioinformatica Camino La piramide 5750 Huechuraba, Santiago de Chile, 8580000, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Manque
authors.affiliation: Virginia Commonwealth University - Center for the Study of Biological Complexity 1101 E. Marshall St., Virginia 23298-0678, US.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Buck
authors.affiliation: Grupo de Parasitología, Facultad de Medicina, Universidad de Antioquia Carrera 53 No. 61-30, Medellin, Antioquia 05001, Colombia.|Centro Nacional de Secuenciación Genómica-CNSG, Universidad de Antioquia Carrera 53 No. 61-30, Medellin, Antioquia 05001, Colombia.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Alzate
mesh_terms: D019295:Computational Biology / Q000379:methods; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics*; D016785:Cryptosporidium parvum / Q000235:genetics; D030541:Databases, Genetic; D063990:Gene Ontology; D018503:Genome, Protozoan; D023281:Genomics / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D058977:Molecular Sequence Annotation; D058889:Molecular Typing; D010802:Phylogeny