title: Transcriptome Analysis of Kiwifruit (Actinidia chinensis) Bark in Response to Armoured Scale Insect (Hemiberlesia lataniae) Feeding.
abstract: The kiwifruit cultivar Actinidia chinensis 'Hort16A' is resistant to the polyphagous armoured scale insect pest Hemiberlesia lataniae (Hemiptera: Diaspididae). A cDNA microarray consisting of 17,512 unigenes selected from over 132,000 expressed sequence tags (ESTs) was used to measure the transcriptomic profile of the A. chinensis 'Hort16A' canes in response to a controlled infestation of H. lataniae. After 2 days, 272 transcripts were differentially expressed. After 7 days, 5,284 (30%) transcripts were differentially expressed. The transcripts were grouped into 22 major functional categories using MapMan software. After 7 days, transcripts associated with photosynthesis (photosystem II) were significantly down-regulated, while those associated with secondary metabolism were significantly up-regulated. A total of 643 transcripts associated with response to stress were differentially expressed. This included biotic stress-related transcripts orthologous with pathogenesis related proteins, the phenylpropanoid pathway, NBS-LRR (R) genes, and receptor-like kinase-leucine rich repeat signalling proteins. While transcriptional studies are not conclusive in their own right, results were suggestive of a defence response involving both ETI and PTI, with predominance of the SA signalling pathway. Exogenous application of an SA-mimic decreased H. lataniae growth on A. chinensis 'Hort16A' plants in two laboratory experiments.
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: M Garry
authors.initials: MG
authors.lastname: Hill
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 3230, Waikato Mail Centre, Hamilton, New Zealand.
authors.forename: Kirstin V
authors.initials: KV
authors.lastname: Wurms
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Marcus W
authors.initials: MW
authors.lastname: Davy
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 3230, Waikato Mail Centre, Hamilton, New Zealand.
authors.forename: Elaine
authors.initials: E
authors.lastname: Gould
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 92169, Auckland, New Zealand.
authors.forename: Andrew
authors.initials: A
authors.lastname: Allan
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Nicola A
authors.initials: NA
authors.lastname: Mauchline
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 92169, Auckland, New Zealand.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 3230, Waikato Mail Centre, Hamilton, New Zealand.
authors.forename: Annette
authors.initials: A
authors.lastname: Ah Chee
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Kate
authors.initials: K
authors.lastname: Stannard
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited (PFR), 412 No1 Rd RD2, Te Puke, New Zealand.
authors.forename: Roy D
authors.initials: RD
authors.lastname: Storey
authors.affiliation: Plant & Food Research Limited (PFR), Private Bag 92169, Auckland, New Zealand.
authors.forename: Erik H
authors.initials: EH
authors.lastname: Rikkerink
mesh_terms: D029042:Actinidia / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D057225:Data Mining; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006430:Hemiptera / Q000472:pathogenicity*; D060434:Herbivory; D020411:Oligonucleotide Array Sequence Analysis; D010788:Photosynthesis; D024301:Plant Bark; D057865:Plant Immunity; D016133:Polymerase Chain Reaction; D015398:Signal Transduction; D012984:Software; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary