title: Characterization and biocompatibility of injectable microspheres-loaded hydrogel for methotrexate delivery.
abstract: Injectable thermosensitive hydrogels have widely been studied as drug delivery systems for their minimally invasive administration and localized drug release. However, burst drug release limits clinical applications of such hydrogels. A double-component injectable formulation (microspheres-loaded hydrogel, CMs-CS-HG) was thus fabricated to eliminate the limitation. Gelation temperature, gelation time, complex viscosity and syringeability tests for CMs-CS-HG demonstrated excellent injectability. After injection, the drug-loaded chitosan-based microspheres (CMs) were localized within the hydrogel, leading to localized drug release. Moreover, CMs-CS-HG had good hemocompatibility and histocompatibility, and had non-genotoxicity and non-cytotoxicity to Kunming mice. In addition, both in vitro and in vivo methotrexate (MTX) releasing efficiencies were evaluated, demonstrating long-term sustained MTX release from MTX-loaded CMs-CS-HG. These results showed the double-component CMs-CS-HG not only maintained good injectability and biocompatibility but also prolonged drug-releasing time in comparison with the single-component CS-HG or CMs, suggesting that CMs-CS-HG may be a promising drug delivery system.
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Dang
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China. Electronic address: liucsouc@126.com.
authors.forename: Chengsheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
authors.forename: Jingquan
authors.initials: J
authors.lastname: Yan
authors.affiliation: Shandong Qingdao Health School, 66 Fuzhou Road, Qingdao 266071, PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Wan
authors.affiliation: Qingdao Aorun Biotechnology Co. Ltd., Room 602, Century Mansion, 39 Donghaixi Road, Qingdao 266071, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Fan
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D016503:Drug Delivery Systems / Q000009:adverse effects; D065546:Drug Liberation; D020100:Hydrogels / Q000009:adverse effects / Q000737:chemistry*; D008727:Methotrexate / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051379:Mice; D008863:Microspheres; D011817:Rabbits
chemical_list: D000964:Antimetabolites, Antineoplastic; D001672:Biocompatible Materials; D020100:Hydrogels; D048271:Chitosan; D008727:Methotrexate
keywords: Biocompatibility; Characterization; Chitosan; Chitosan (PubChem CID: 71853); Drug release; Eeosin (PubChem CID: 11048); Giemsa stain (PubChem CID: 13735); Hematoxylin (PubChem CID: 442541); Lactic acid (PubChem CID: 612); Methanol (PubChem CID: 887); Microspheres-loaded hydrogel; Sodium chloride (PubChem CID: 5234); α-Glycerophosphate (PubChem CID: 14754); β-Glycerophosphate (PubChem CID: 6101544)