title: Fluorescent Biotin Analogues for Microstructure Patterning and Selective Protein Immobilization.
abstract: Benzyl substitution on ureido nitrogens of biotin led to manifestation of aggregation-induced emission, which was studied by steady-state fluorescence, microscopy, and TD-DFT, providing a rationale into the observed photophysical behavior. Besides exhibiting solvatochromism, the biotin derivatives revealed emission peaks centered at ∼430 and 545 nm, which has been attributed to the π-π stacking interactions. Our TD-DFT results also correlate the spectroscopic data and quantify the nature of transitions involved. The isothermal titration calorimetry data substantiates that the binding of the biotin derivatives with avidin are pretty strong. These derivatives on lithographic patterning present a platform for site specific strept(avidin) immobilization, thus opening avenues for potential applications exploiting these interactions. The fluorescent biotin derivatives can thus find applications in cellular biology and imaging.
authors.forename: K Vijaya
authors.initials: KV
authors.lastname: Krishna
authors.affiliation: Department of Chemistry, IISER-Bhopal , Bhopal-462066 Madhya Pradesh, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Ghosh
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Sharma
authors.forename: Leeju
authors.initials: L
authors.lastname: Singh
authors.affiliation: Department of Chemistry, IISER-Bhopal , Bhopal-462066 Madhya Pradesh, India.
authors.forename: Saptarshi
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Sandeep
authors.initials: S
authors.lastname: Verma
mesh_terms: D001710:Biotin / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006860:Hydrogen Bonding; D055767:Immobilized Proteins / Q000737:chemistry*; D055616:Microtechnology; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D001710:Biotin