title: Transcriptome Analysis and Development of SSR Molecular Markers in Glycyrrhiza uralensis Fisch.
abstract: Licorice is an important traditional Chinese medicine with clinical and industrial applications. Genetic resources of licorice are insufficient for analysis of molecular biology and genetic functions; as such, transcriptome sequencing must be conducted for functional characterization and development of molecular markers. In this study, transcriptome sequencing on the Illumina HiSeq 2500 sequencing platform generated a total of 5.41 Gb clean data. De novo assembly yielded a total of 46,641 unigenes. Comparison analysis using BLAST showed that the annotations of 29,614 unigenes were conserved. Further study revealed 773 genes related to biosynthesis of secondary metabolites of licorice, 40 genes involved in biosynthesis of the terpenoid backbone, and 16 genes associated with biosynthesis of glycyrrhizic acid. Analysis of unigenes larger than 1 Kb with a length of 11,702 nt presented 7,032 simple sequence repeats (SSR). Sixty-four of 69 randomly designed and synthesized SSR pairs were successfully amplified, 33 pairs of primers were polymorphism in in Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., Glycyrrhiza glabra L. and Glycyrrhiza pallidiflora Maxim. This study not only presents the molecular biology data of licorice but also provides a basis for genetic diversity research and molecular marker-assisted breeding of licorice.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102, PR China.|College of Life Science, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, PR China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Life Science, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shanxi Agricultural University, Taigu, 030801, Shanxi Province, PR China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Song
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102, PR China.
authors.forename: Junling
authors.initials: J
authors.lastname: Hou
authors.affiliation: School of Inner Mongolia Medical University, Hohhot, 010059, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qing
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102, PR China.|Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, PR China.
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102, PR China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
mesh_terms: D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D063990:Gene Ontology; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D029882:Glycyrrhiza uralensis / Q000235:genetics* / Q000378:metabolism; D059014:High-Throughput Nucleotide Sequencing; D053858:Metabolic Networks and Pathways; D018895:Microsatellite Repeats; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D018749:RNA, Plant