title: Body Composition QTLs Identified in Intercross Populations Are Reproducible in Consomic Mouse Strains.
abstract: Genetic variation contributes to individual differences in obesity, but defining the exact relationships between naturally occurring genotypes and their effects on fatness remains elusive. As a step toward positional cloning of previously identified body composition quantitative trait loci (QTLs) from F2 crosses of mice from the C57BL/6ByJ and 129P3/J inbred strains, we sought to recapture them on a homogenous genetic background of consomic (chromosome substitution) strains. Male and female mice from reciprocal consomic strains originating from the C57BL/6ByJ and 129P3/J strains were bred and measured for body weight, length, and adiposity. Chromosomes 2, 7, and 9 were selected for substitution because previous F2 intercross studies revealed body composition QTLs on these chromosomes. We considered a QTL confirmed if one or both sexes of one or both reciprocal consomic strains differed significantly from the host strain in the expected direction after correction for multiple testing. Using these criteria, we confirmed two of two QTLs for body weight (Bwq5-6), three of three QTLs for body length (Bdln3-5), and three of three QTLs for adiposity (Adip20, Adip26 and Adip27). Overall, this study shows that despite the biological complexity of body size and composition, most QTLs for these traits are preserved when transferred to consomic strains; in addition, studying reciprocal consomic strains of both sexes is useful in assessing the robustness of a particular QTL.
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Cailu
authors.initials: C
authors.lastname: Lin
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Brad D
authors.initials: BD
authors.lastname: Fesi
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Marquis
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Bosak
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Theodorides
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Avigdor
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Amanda H
authors.initials: AH
authors.lastname: McDaniel
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Fujiko F
authors.initials: FF
authors.lastname: Duke
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Anna
authors.initials: A
authors.lastname: Lysenko
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Amin
authors.initials: A
authors.lastname: Khoshnevisan
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Brian R
authors.initials: BR
authors.lastname: Gantick
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Arayata
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Theodore M
authors.initials: TM
authors.lastname: Nelson
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Bachmanov
authors.affiliation: Monell Chemical Senses Center, Philadelphia, PA, 19104, United States of America.
authors.forename: Danielle R
authors.initials: DR
authors.lastname: Reed
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D001827:Body Height / Q000235:genetics*; D049628:Body Size / Q000235:genetics*; D001835:Body Weight / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000235:genetics; D010641:Phenotype; D040641:Quantitative Trait Loci / Q000235:genetics*