title: MicroRNA124 Regulated Neurite Elongation by Targeting OSBP.
abstract: MicroRNA-124 (miR-124), a brain-specific miRNA molecule, has been implicated in stimulating neurite outgrowth and elongation during neuronal differentiation. However, the direct target genes and the mechanisms of miR-124-induced neurite outgrowth are poorly understood. In this study, we demonstrated that miR-124 directly targeted and downregulated the endogenous expression of oxysterol-binding protein (OSBP). A previous study found that the expression of miR-124 increased during brain development. In the present study, we demonstrated that the expression of OSBP decreased during the development of the C57BL/6 mouse cortex, which was negatively correlated with miR-124 expression. OSBP knockdown using specific shRNAs promoted neurite outgrowth and elongation in both Neuro-2a cells and primary cultured mouse cortical neurons. Conversely, OSBP overexpression strongly repressed the neurite elongation-enhancing effect of miR-124 in Neuro-2a cells. Our results suggested that OSBP may be a target and downstream effector of miR-124 for regulating neurite outgrowth and elongation.
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Gu
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Aili
authors.initials: A
authors.lastname: Li
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Shuhu
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Lin
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Peidong
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Shuji
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Tian
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Psychiatric Disorders of Guangdong Province, Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, People's Republic of China. xmwang@smu.edu.cn.|Department of Neurobiology, Southern Medical University, Guangzhou, Guangdong Province, China. xmwang@smu.edu.cn.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D015536:Down-Regulation / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism*; D000071446:Neuronal Outgrowth / Q000235:genetics; D034741:RNA, Small Interfering / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D000098825:Oxysterol Binding Proteins
chemical_list: D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse; D034741:RNA, Small Interfering; D011987:Receptors, Steroid; D000098825:Oxysterol Binding Proteins
keywords: Brain development; Neurite elongation; OSBP; miR-124