title: Cell-free plasma hemoglobin removal by dialyzers with various permeability profiles.
abstract: The release of hemoglobin from mechanically stressed erythrocytes into plasma is a general side effect of extracorporeal therapies, such as extracorporeal membrane oxygenation or hemodialysis. In many reported cases dialysis patients showed elevated cell-free plasma hemoglobin (CPH) levels which are associated with pathophysiological effects. In this in vitro study, the CPH clearance capacity of various filters with different permeability profiles was measured. Simulated dialysis treatments were conducted and clearance was calculated from variations in CPH concentrations over time by measuring plasma absorbance at 405 nm. Conventional high-flux filters exhibited no detectable clearance of CPH. High-flux filters with extended permeability exhibited clearances between 5.8 ± 1.2 and 12.7 ± 1.7 ml/min when tested with plasma and between 5.8 ± 1.2 and 11.3 ± 1.6 ml/min when tested with whole blood. septeX high-cutoff filters had clearances between 13.8 ± 1.8 and 15.5 ± 1.7 ml/min when tested with plasma and of 22.6 ± 2.9 ml/min when tested with whole blood. This study demonstrated that filters with extended permeability and the septeX filter enable CPH removal when used as in chronic and acute settings.
authors.affiliation: Gambro Dialysatoren GmbH (part of Baxter International Inc.), Research &Development, 72379 Hechingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hulko
authors.affiliation: Gambro Dialysatoren GmbH (part of Baxter International Inc.), Research &Development, 72379 Hechingen, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Kunz
authors.affiliation: Gambro Dialysatoren GmbH (part of Baxter International Inc.), Research &Development, 72379 Hechingen, Germany.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yildirim
authors.affiliation: Gambro Dialysatoren GmbH (part of Baxter International Inc.), Research &Development, 72379 Hechingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Homeyer
authors.affiliation: University Hospital Tuebingen, Pediatric Nephrology, 72076 Tuebingen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Amon
authors.affiliation: Gambro Dialysatoren GmbH (part of Baxter International Inc.), Research &Development, 72379 Hechingen, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Krause
mesh_terms: D003956:Dialysis / Q000295:instrumentation*; D006454:Hemoglobins / Q000737:chemistry; D006801:Humans; D008567:Membranes, Artificial; D010539:Permeability; D010949:Plasma / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D008567:Membranes, Artificial