title: [Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region].
abstract: OBJECTIVE
  To investigate the relationship between hepatitis B virus (HBV) genotype, the mutation in basic core promoter (BCP) region/pre-core (Pre-C) region and the incidence of hepatocellular carcinoma (HCC) in Fusui county of Guangxi Zhuang Autonomous Region (Guangxi), a area with high incidence of HCC.
  
  
  METHODS
  In this case-control study, 53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype, gene mutation of HBV and the incidence of HCC was analyzed.
  
  
  RESULTS
  The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group (94.3% vs. 75.7%, P = 0.006; 50.9% vs. 31.4%, P = 0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%) (P = 0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC (OR = 5.459, 95% CI: 1.397-21.332, P = 0.015; OR = 3.881, 95% CI: 1.462-10.305, P = 0.006). A1775G is the protective factor in the development of HCC (OR = 0.192, 95% CI: 0.059-0.622, P = 0.006).
  
  
  CONCLUSIONS
  The present investigation showed that BCP A1762T/G1764A, A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
authors.affiliation: Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China; Email: tianrenhuang@sina.com.
authors.forename: Tianren
authors.initials: T
authors.lastname: Huang
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangxi Medical University, Nanning 530021, China.
authors.forename: Zhaofa
authors.initials: Z
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000821:virology*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D004279:DNA, Viral; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008113:Liver Neoplasms / Q000453:epidemiology / Q000821:virology*; D009154:Mutation; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral