title: Quantitative image analysis as a tool for Yarrowia lipolytica dimorphic growth evaluation in different culture media.
abstract: Yarrowia lipolytica, a yeast strain with a huge biotechnological potential, capable to produce metabolites such as γ-decalactone, citric acid, intracellular lipids and enzymes, possesses the ability to change its morphology in response to environmental conditions. In the present study, a quantitative image analysis (QIA) procedure was developed for the identification and quantification of Y. lipolytica W29 and MTLY40-2P strains dimorphic growth, cultivated in batch cultures on hydrophilic (glucose and N-acetylglucosamine (GlcNAc) and hydrophobic (olive oil and castor oil) media. The morphological characterization of yeast cells by QIA techniques revealed that hydrophobic carbon sources, namely castor oil, should be preferred for both strains growth in the yeast single cell morphotype. On the other hand, hydrophilic sugars, namely glucose and GlcNAc caused a dimorphic transition growth towards the hyphae morphotype. Experiments for γ-decalactone production with MTLY40-2P strain in two distinct morphotypes (yeast single cells and hyphae cells) were also performed. The obtained results showed the adequacy of the proposed morphology monitoring tool in relation to each morphotype on the aroma production ability. The present work allowed establishing that QIA techniques can be a valuable tool for the identification of the best culture conditions for industrial processes implementation.
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: A
authors.initials: A
authors.lastname: Braga
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: D P
authors.initials: DP
authors.lastname: Mesquita
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal; Instituto Politécnico de Coimbra, ISEC, DEQB, Rua Pedro Nunes, Quinta da Nora, 3030-199 Coimbra, Portugal.
authors.forename: A L
authors.initials: AL
authors.lastname: Amaral
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
authors.forename: E C
authors.initials: EC
authors.lastname: Ferreira
authors.affiliation: CEB-Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address: ibelo@deb.uminho.pt.
authors.forename: I
authors.initials: I
authors.lastname: Belo
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D025301:Hyphae / Q000166:cytology / Q000254:growth & development; D009172:Mycology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods; D025062:Yarrowia / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D002244:Carbon; D009584:Nitrogen
keywords: Dimorphic growth; Quantitative image analysis; Y. lipolytica; γ-Decalactone