title: Protein labeling in Escherichia coli with (2)H, (13)C, and (15)N.
abstract: A number of structural biology techniques such as nuclear magnetic resonance spectroscopy and small-angle neutron scattering can be performed with proteins with nuclei at natural isotope abundance. However, the use of proteins labeled with stable isotopes ((2)H, (13)C, and (15)N) enables greater experimental flexibility. In this chapter, several methods for uniform and fractional protein labeling with stable isotopes using Escherichia coli in a defined media are described. The methods described can be used for labeling with single or multiple isotopes.
authors.affiliation: Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA. Electronic address: hoopesj@umd.edu.
authors.forename: J Todd
authors.initials: JT
authors.lastname: Hoopes
authors.affiliation: Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Elberson
authors.affiliation: Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
authors.forename: Renae J
authors.initials: RJ
authors.lastname: Preston
authors.affiliation: Biomolecular Labeling Laboratory, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA; Biomolecular Labeling Laboratory, and Biomolecular Structure and Function Group, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
authors.forename: Prasad T
authors.initials: PT
authors.lastname: Reddy
authors.affiliation: Biomolecular Labeling Laboratory, and Biomolecular Structure and Function Group, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.
authors.forename: Zvi
authors.initials: Z
authors.lastname: Kelman
mesh_terms: D000222:Adaptation, Physiological; D002247:Carbon Isotopes; D003903:Deuterium; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins / Q000737:chemistry*; D007553:Isotope Labeling; D009587:Nitrogen Isotopes; D010957:Plasmids
chemical_list: D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D009587:Nitrogen Isotopes; D003903:Deuterium
keywords: (13)C; (15)N; (2)H; Deuterium; E. coli; Protein expression; Stable isotope labeling