title: The involvement of myocyte enhancer factor 2D in regulating tumor biology of cardiac myxoma.
abstract: The pro-survival transcription factor myocyte enhancer factor 2D (MEF2D) is identified to exhibit pro-tumor effects based on clinical and experimental studies. However, the detailed mechanisms underlying IGF-1-MEF2D pathway-induced tumor biology in cardiac myxoma (CM) was not clear. Here, we investigated the role of MEF2D in CM tissues and cells using RT-PCR, western blot, gene silencing, et al. Our findings revealed MEF2D was significantly increased in CM tissues compared with adjacent normal tissues and closely related to tumor size. In vitro assay demonstrated that IGF-1 enhanced CM cell proliferation in a time-dependent fashion. However, knockdown of MEF2D reversed the IGF-1-induced proliferative effects on CM cells in a time-dependent fashion and further resulted in cell cycle arrest. Based on the molecular level, IGF-1 enhanced the expression of epidermal growth factor receptor (EGFR) and matrix metalloprotein 9 (MMP9) in CM cells, whereas knockdown of MEF2D was able to reduce the expression of EGFR and MMP9 compared with vector control. Furthermore, we found knockdown of MEF2D directly affected G1/S transition in cultured CM cells. In conclusion, MEF2D regulates IGF-1-induced proliferation and apoptosis in CM development, indicating IGF-1-MEF2D pathway may be a useful target for treatment.
authors.affiliation: Department of Cardiac Surgery, Qingdao Branch, Qilu Hospital of Shandong University, No. 758 Hefei Road, Shibei District, Qingdao, Shandong, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Huo
authors.affiliation: Department of Anesthesia, ZhangQiu People's Hospital, No. 1920, Huiquan Road, Zhangqiu, Shandong, China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Cardiothoracic Surgery, JiMo People's Hospital, Qingdao, Shandong, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cardiac Surgery, Qingdao Branch, Qilu Hospital of Shandong University, No. 758 Hefei Road, Shibei District, Qingdao, Shandong, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Cardiac Surgery, Qingdao Branch, Qilu Hospital of Shandong University, No. 758 Hefei Road, Shibei District, Qingdao, Shandong, China.
authors.forename: Yinghai
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Cardiac Surgery, Qilu Hospital of Shandong University, No. 107 West Wenhua Road, 250012, Jinan, China. sunwenyumd@163.com.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006338:Heart Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics*; D064326:MEF2 Transcription Factors / Q000235:genetics; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D008875:Middle Aged; D009232:Myxoma / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction
chemical_list: D064326:MEF2 Transcription Factors; C578043:MEF2D protein, human; D007334:Insulin-Like Growth Factor I; C512478:EGFR protein, human; D066246:ErbB Receptors; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Biology; CM; IGF-1; MEF2D