title: Fluorescence study on the aggregation of collagen molecules in acid solution influenced by hydroxypropyl methylcellulose.
abstract: The effect of hydroxypropyl methylcellulose (HPMC) on the aggregation of collagen molecules with collagen concentrations of 0.25, 0.5 and 1.0mg/mL was studied by fluorescence techniques. On one hand, both the synchronous fluorescence spectra and fluorescence emission spectra showed that there was no change in the fluorescence intensity of collagen intrinsic fluorescence when 30% HPMC was added, while it decreased obviously when HPMC content ≥ 50%. From the two-dimensional fluorescence correlation analysis, it was indicated that collagen molecules in 0.25 and 0.5mg/mL collagen solutions were more sensitive to HPMC than those in 1.0mg/mL collagen solution. On the other hand, the pyrene fluorescence and the fluorescence anisotropy measurements indicated that HPMC inhibited the collagen aggregation for 0.25 and 0.5mg/mL collagen, but promoted it for 1.0mg/mL collagen. The atomic force microscopy images further confirmed the effect of HPMC on collagen with different initial states.
authors.affiliation: The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, PR China; College of Ecological Environment and Urban Construction, Fujian University of Technology, Fuzhou 350108, PR China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Ding
authors.affiliation: College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, PR China; National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, PR China. Electronic address: liguoyings@163.com.
authors.forename: Guoying
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D005454:Fluorescence Polarization; D006863:Hydrogen-Ion Concentration; D065347:Hypromellose Derivatives / Q000494:pharmacology*; D066329:Protein Aggregates; D011721:Pyrenes / Q000737:chemistry; D012996:Solutions
chemical_list: D066329:Protein Aggregates; D011721:Pyrenes; D012996:Solutions; D065347:Hypromellose Derivatives; D003094:Collagen; C030984:pyrene
keywords: Aggregation; Collagen; Fluorescence; Hydroxypropyl methylcellulose