title: [Effects of NK-1R inhibitor WIN62577 on the migration of airway smooth muscle cells in asthmatic rats with airway remodeling].
abstract: OBJECTIVE
  To study the changes in the migration of airway smooth muscle cells (ASMC) in asthmatic rats with airway remodeling and the effect of NK-1R inhibitor WIN62577 on the migration of ASMC.
  
  
  METHODS
  Sprague-Dawley rats were randomly assigned into two groups: airway remodeling induced by asthma and normal control. ASMC from rats with asthma and airway remodeling induced by ovalbumin (OVA) inhalation for 8 weeks were primary cultured and purified. Immunofluorescence and real-time PCR were used to measure the expression of NK-1R. With NK-1R inhibitor WIN62577 treatment, the changes in the migration of ASMC were measured by transwell chambers.
  
  
  RESULTS
  NK-1R in ASMC was expressed mainly in the cytoplasm and cell membrane in the airway remodeling group, and the mRNA expression of NK-1R was higher than the normal control group (P<0.01). The number of the migrated ASMC in the airway remodeling group was significantly higher than that in the normal control group (P<0.01). Various concentrations (10-11 mol/L, 10-10 mol/L, 10-9 mol/L and 10-8 mol/L) of WIN62577 treatment decreased the number of the migrated ASMC (P<0.05).
  
  
  CONCLUSIONS
  NK-1R may affect airway remodeling possibly through promoting the migration ability of ASMC in rats with asthma.
authors.affiliation: Department of Pediatrics, General Hospital of Shenyang Military Area Command, Shenyang 110016, China. weib71@sina.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Wei
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Yun-Xiao
authors.initials: YX
authors.lastname: Shang
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Li
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects*; D000736:Androstenes / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000473:pathology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D064729:Neurokinin-1 Receptor Antagonists / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000736:Androstenes; D001562:Benzimidazoles; D064729:Neurokinin-1 Receptor Antagonists; C086110:WIN 62577