title: Rho GDIalpha Modulates Rabbit Trophoblast Stem Cell Survival and Migration.
abstract: Trophoblast stem cells differentiate into different trophoblast cell populations that are indispensable for successful pregnancy through interactions with the maternal uterine decidua. Rho GTPases play an important role in the regulation of trophoblast stem cell (TSC) self-renewal and differentiation; however, the role of Rho GDP-dissociation inhibitors (Rho GDIs) remains unclear. Here we report that overexpression of Rho GDIalpha resulted in rapid apoptosis of TSCs, while its knockdown promoted proliferation. Moreover, Rho GDIalpha knockdown also enhanced TSC invasion. Collectively, these results establish a potential mechanism whereby TSCs can balance growth and apoptosis, and thus ensure normal fetal development.
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.
authors.forename: Tianjie
authors.initials: T
authors.lastname: Li
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Ji
authors.affiliation: Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China yuyang5012@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Yunnan Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan, China tant@kbimed.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Tan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D005260:Female; D011247:Pregnancy; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D062948:rho Guanine Nucleotide Dissociation Inhibitor alpha / Q000378:metabolism*
chemical_list: D062948:rho Guanine Nucleotide Dissociation Inhibitor alpha
keywords: Rho GDIα; apoptosis; cytoskeleton; gene expression; gene regulation; invasion; stem cells; trophoblast