title: Chemical Composition and Medicinal Value of the New Ganoderma tsugae var. jannieae CBS-120304 Medicinal Higher Basidiomycete Mushroom.
abstract: In this research, the chemical composition and anticancer and antioxidant activity of the new medicinal mushroom Ganoderma tsugae var. jannieae CBS-120304 were evaluated. The chemical composition assay includes amounts of total carbohydrates and proteins, amino acids, fatty acids, micro- and macroelements, and vitamins. The investigated medicinal mushroom seemed to be a rich source of nutritional components. Mycelium accumulated more than 2-fold more total protein compared with the fruiting body and reached 37% and 16% of dry weight, respectively. Carbohydrate content in the fruiting body seemed to be conspicuously higher than in the mycelium (50% of dry weight) and reached 80% of dry weight. Quantification of the identified fatty acids indicated that, in general, palmitic acid, oleic acid, and linoleic acid were the major fatty acids. Toxic elements, such as silver, arsenic, cadmium, and mercury, were found only in trace amounts in mycelium and were not detected in the fruiting body. Furthermore, the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay was used to evaluate antioxidant activity. The highest radical scavenging activity was 9.0 mg/mL (65.9%) by ethanol extract. In addition, mycelial extracts were tested to inhibit MCF7 breast cancer cells. Ganoderma tsugae var. jannieae ethyl acetate extract (GTEAE) extract showed high potential by inhibiting reporter activity by more than 70%. Results demonstrated that GTEAE had a strong effect on inhibitory protein κΒα level in the higher concentration used (200 gg/mL), which could be compared with the effect of parthenolide. Furthermore, GTEAE demonstrated strong inhibition of IκΒα phosphorylation.
authors.affiliation: Phyto Pharma Ltd., Singapore.
authors.forename: Jannie Siew Lee
authors.initials: JS
authors.lastname: Chan
authors.affiliation: Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences & Institute of Evolution, University of Haifa; Department of Anatomy and Cell Biology, Faculty of Medicine, Technion, Haifa; Agricultural University of Georgia, Tbilisi.
authors.forename: Mikheil D
authors.initials: MD
authors.lastname: Asatiani
authors.affiliation: Department of Evolutionary and Environmental Biology and Institute of Evolution, Faculty of Natural Sciences, University of Haifa, Mount Carmel, Haifa 31905, Israel.
authors.forename: Lital E
authors.initials: LE
authors.lastname: Sharvit
authors.affiliation: Department of Evolutionary and Environmental Biology and Department of Human Biology, Faculty of Natural Sciences, University of Haifa, Mt. Carmel, Haifa, Israel.
authors.forename: Beny
authors.initials: B
authors.lastname: Trabelcy
authors.affiliation: Institute of Evolution and Department of Evolutionary & Environmental Biology, Faculty of Natural Sciences, University of Haifa, Mt. Carmel, Haifa 31905, Israel.
authors.forename: Gayane S
authors.initials: GS
authors.lastname: Barseghyan
authors.affiliation: International Centre for Cryptogamic Plants and Fungi, Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, Israel; and N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev, Ukraine.
authors.forename: Solomon P
authors.initials: SP
authors.lastname: Wasser
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002681:China; D004354:Drug Screening Assays, Antitumor; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D032902:Ganoderma / Q000737:chemistry*; D006801:Humans; D025282:Mycelium
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants