title: Genome-wide Screening of Regulators of Catalase Expression: ROLE OF A TRANSCRIPTION COMPLEX AND HISTONE AND tRNA MODIFICATION COMPLEXES ON ADAPTATION TO STRESS.
abstract: In response to environmental cues, the mitogen-activated protein kinase Sty1-driven signaling cascade activates hundreds of genes to induce a robust anti-stress cellular response in fission yeast. Thus, upon stress imposition Sty1 transiently accumulates in the nucleus where it up-regulates transcription through the Atf1 transcription factor. Several regulators of transcription and translation have been identified as important to mount an integral response to oxidative stress, such as the Spt-Ada-Gcn5-acetyl transferase or Elongator complexes, respectively. With the aim of identifying new regulators of this massive gene expression program, we have used a GFP-based protein reporter and screened a fission yeast deletion collection using flow cytometry. We find that the levels of catalase fused to GFP, both before and after a threat of peroxides, are altered in hundreds of strains lacking components of chromatin modifiers, transcription complexes, and modulators of translation. Thus, the transcription elongation complex Paf1, the histone methylase Set1-COMPASS, and the translation-related Trm112 dimers are all involved in full expression of Ctt1-GFP and in wild-type tolerance to peroxides.
authors.affiliation: From the Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: García
authors.affiliation: From the Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Encinar Del Dedo
authors.affiliation: From the Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain jose.ayte@upf.edu.
authors.forename: José
authors.initials: J
authors.lastname: Ayté
authors.affiliation: From the Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain elena.hidalgo@upf.edu.
authors.forename: Elena
authors.initials: E
authors.lastname: Hidalgo
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D002374:Catalase / Q000378:metabolism*; D005434:Flow Cytometry; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal; D017930:Genes, Reporter; D005820:Genetic Testing; D016681:Genome, Fungal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D046912:Multiprotein Complexes / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D014176:Protein Biosynthesis / Q000187:drug effects; D012343:RNA, Transfer / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D006657:Histones; D046912:Multiprotein Complexes; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D012343:RNA, Transfer; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: Schizosaccharomyces pombe; catalase; gene expression; stress response; transfer RNA (tRNA)