title: Development and Validation of an UPLC-Q-TOF-MS Method for Quantification of Fuziline in Beagle Dog After Intragastric and Intravenous Administration.
abstract: A specific and sensitive UPLC-Q-TOF-MS method operated in the positive ion mode was developed and validated for the quantification of Fuziline in Beagle dog plasma. Fuziline and Neoline internal standard were separated on an Acquity UPLC BEH C18 column with the total running time of 4 min using gradient elution at the flow rate of 0.25 mL/min. The calibration curves for Fuziline showed good linearity in the concentrations ranging from 2 to 400 ng/mL with correlation coefficients (r) greater than 0.9971. The lower limit of quantification was 0.8 ng/mL. Intra- and interbatch relative standard deviations ranged from 2.11 to 3.11% and 3.12 to 3.81%, respectively. Fuziline was stable under different sample storage and processing conditions. The developed method was successfully applied to the comparative pharmacokinetic study of Fuziline in Beagle dog after intravenous and oral administration. Low absolute bioavailability of Fuziline (1.45 ± 0.76%) suggested a significant metabolism transformation extent in Beagle dog.
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Gong
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: Yun-xia
authors.initials: YX
authors.lastname: Li
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: An
authors.initials: A
authors.lastname: Yuan
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: Meng-jie
authors.initials: MJ
authors.lastname: Zhao
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China.
authors.forename: Ruo-qi
authors.initials: RQ
authors.lastname: Zhang
authors.affiliation: Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, People's Republic of China.
authors.forename: Dai-wen
authors.initials: DW
authors.lastname: Zeng
authors.affiliation: Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, People's Republic of China State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 610075, People's Republic of China pengchengsub@126.com.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000097:blood; D061605:Administration, Intravenous; D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001682:Biological Availability; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004285:Dogs; D057230:Limit of Detection; D015588:Observer Variation; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards; D013729:Terpenes / Q000097:blood / Q000493:pharmacokinetics*
chemical_list: D000470:Alkaloids; D013729:Terpenes; C487396:neoline; D000157:Aconitine