title: Complete genome sequence of novel T7-like virus vB_KpnP_KpV289 with lytic activity against Klebsiella pneumoniae.
abstract: A novel bacteriophage, vB_KpnP_KpV289, lytic for hypermucoviscous strains of Klebsiella pneumoniae, was attributed to the family Podoviridae, subfamily Autographivirinae, genus T7likevirus based on transmission electron microscopy and genome analysis. The complete genome of the bacteriophage vB_KpnP_KpV289 consists of a linear double-stranded DNA of 41,054 bp including 179-bp direct-repeat sequences at the ends and 51 open reading frames (ORFs). The G+C content is 52.56 %. The phage was shown to lyse 15 out of 140 (10.7 %) K. pneumoniae strains belonged to the capsular types K-1, K-2, and K-57 and strains without a determined capsular type, including a hypermucoviscous strain of the novel sequence type ST-1554.
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation. nikvol@obolensk.org.
authors.forename: Nikolay V
authors.identifier: http://orcid.org/0000-0003-4667-1451
authors.initials: NV
authors.lastname: Volozhantsev
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Vera P
authors.initials: VP
authors.lastname: Myakinina
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Popova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Angelina A
authors.initials: AA
authors.lastname: Kislichkina
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Komisarova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Anastasia I
authors.initials: AI
authors.lastname: Knyazeva
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Valentina M
authors.initials: VM
authors.lastname: Krasilnikova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Nadezhda K
authors.initials: NK
authors.lastname: Fursova
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russian Federation.
authors.forename: Eduard A
authors.initials: EA
authors.lastname: Svetoch
mesh_terms: D001433:Bacteriolysis; D001482:Base Composition; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D007711:Klebsiella pneumoniae / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017902:Podoviridae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral