title: High-resolution NMR-based metabolic detection of microgram biopsies using a 1 mm HRμMAS probe.
abstract: A prototype 1 mm High-Resolution micro-Magic Angle Spinning (HRμMAS) probe is described. High quality (1)H NMR spectra were obtained from 490 μg of heterogeneous biospecimens, offering a rich-metabolite profiling. The results demonstrate the potential of HRμMAS as a new NMR analytical tool in metabolomics.
authors.affiliation: JEOL RESONANCE Inc., 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan and RIKEN CLST-JEOL Collaboration Center, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nishiyama
authors.affiliation: JEOL RESONANCE Inc., 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Endo
authors.affiliation: JEOL RESONANCE Inc., 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nemoto
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, CNRS-Université de Bordeaux, UMR5536, Bordeaux, France.
authors.forename: Anne-Karine
authors.initials: AK
authors.lastname: Bouzier-Sore
authors.affiliation: CEA Saclay, DSM, IRAMIS, CEA/CNRS UMR3685-NIMBE, Laboratoire Structure et Dynamiquepar Reśonance Magnetique, F-91191, Gif-sur-Yvette Cedex, France. alan.wong@cea.fr.
authors.forename: Alan
authors.initials: A
authors.lastname: Wong
mesh_terms: D001706:Biopsy / Q000295:instrumentation*; D001921:Brain / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics / Q000295:instrumentation*; D015335:Molecular Probes / Q000737:chemistry
chemical_list: D015335:Molecular Probes