title: Plasma biomarker for detection of early stage pancreatic cancer and risk factors for pancreatic malignancy using antibodies for apolipoprotein-AII isoforms.
abstract: We recently reported that circulating apolipoprotein AII (apoAII) isoforms apoAII-ATQ/AT (C-terminal truncations of the apoAII homo-dimer) decline significantly in pancreatic cancer and thus might serve as plasma biomarkers for the early detection of this disease. We report here the development of novel enzyme-linked immunosorbent assays (ELISAs) for measurement of apoAII-ATQ/AT and their clinical applicability for early detection of pancreatic cancer. Plasma and serum concentrations of apoAII-ATQ/AT were measured in three independent cohorts, which comprised healthy control subjects and patients with pancreatic cancer and gastroenterologic diseases (n = 1156). These cohorts included 151 cases of stage I/II pancreatic cancer. ApoAII-ATQ/AT not only distinguished the early stages of pancreatic cancer from healthy controls but also identified patients at high risk for pancreatic malignancy. AUC values of apoAII-ATQ/AT to detect early stage pancreatic cancer were higher than those of CA19-9 in all independent cohorts. ApoAII-ATQ/AT is a potential biomarker for screening patients for the early stage of pancreatic cancer and identifying patients at risk for pancreatic malignancy (161 words).
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.|Japan Agency for Medical Research and Development (AMED) CREST, Tokyo 100-0004, Japan.
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Honda
authors.affiliation: Toray Industries, Inc., New Frontiers Research Labs, Kanagawa 248-8555, Japan.
authors.forename: Michimoto
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Hepatobiliary and Pancreatic Oncology Division, National Cancer Center Hospital, Tokyo 104-0045, Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Okusaka
authors.affiliation: National Cancer Institute, Division of Cancer Prevention, Rockville, MD 20852, USA.
authors.forename: Jo Ann
authors.initials: JA
authors.lastname: Rinaudo
authors.affiliation: Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle 98109-1024, WA, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle 98109-1024, WA, USA.
authors.forename: Tracey
authors.initials: T
authors.lastname: Marsh
authors.affiliation: Toray Industries, Inc., New Frontiers Research Labs, Kanagawa 248-8555, Japan.
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Sanada
authors.affiliation: Toray Industries, Inc., New Frontiers Research Labs, Kanagawa 248-8555, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Sasajima
authors.affiliation: Department of Surgery, Osaka National Hospital, National Hospital Organization, Osaka 540-0006, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Nakamori
authors.affiliation: Department of Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Shimahara
authors.affiliation: Department of Oral Surgery, Osaka Medical College, Osaka 569-8686, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ueno
authors.affiliation: Department of Gastrointestinal and Pediatric Surgery, Tokyo Medical University, Tokyo 160-0023, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tsuchida
authors.affiliation: Department of Surgery, Jichi Medical University, Tochigi 329-0498, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Sata
authors.affiliation: Department of Hepatobiliary and Pancreatic Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537-0025, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ioka
authors.affiliation: Department of Regenerative Medicine and Transplantation, Fukuoka University Faculty of Medicine, Fukuoka 814-0018, Japan.
authors.forename: Yohichi
authors.initials: Y
authors.lastname: Yasunami
authors.affiliation: Hepatobiliary and Pancreatic Surgery Division, National Cancer Center Hospital, Tokyo 104-0045, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Kosuge
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Nami
authors.initials: N
authors.lastname: Miura
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kamita
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Shoji
authors.affiliation: Toray Industries, Inc., New Frontiers Research Labs, Kanagawa 248-8555, Japan.
authors.forename: Giman
authors.initials: G
authors.lastname: Jung
authors.affiliation: National Cancer Institute, Division of Cancer Prevention, Rockville, MD 20852, USA.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Division of Chemotherapy and Clinical Research, National Cancer Center Research Institute, Tokyo 104-0045, Japan.
authors.forename: Tesshi
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies / Q000276:immunology; D016633:Apolipoprotein A-II / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D055088:Early Detection of Cancer; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000473:pathology; D012307:Risk Factors
chemical_list: D000906:Antibodies; D016633:Apolipoprotein A-II; D014408:Biomarkers, Tumor