title: NF-κB protects human neuroblastoma cells from nitric oxide-induced apoptosis through upregulating biglycan.
abstract: Excessive nitric oxide (NO) produced in inflammation may result in oxidative stress, which is closely related to the neurodegenerative diseases and brain damage. Massive NO production can enhance NF-κB activity in various neural cells, but the function of this activation by NO and the target genes transactivated by NF-κB are still largely unknown. In the present study, our results showed sodium nitropruside (SNP), a NO donor, triggered apoptotic cell death and NF-κB activation in human neuroblastoma SH-EP1 cells, and inhibition of NF-κB activation by its super endogenous inhibitor, I-κBαM, sensitized SH-EP1 cells to NO-induced apoptosis. Conversely, NF-κB activation induced by insulin-like growth factor (IGF)-1 antagonizes NO-induced apoptotic cell death in SH-EP1 cells. In addition, cDNA microarray analysis showed biglycan, an extracellular glycoprotein, was up-regulated by NF-κB, and recombinant biglycan protein conferred a protective effect on NF-κB mediated NO-induced apoptotic cell death in SH-EP1 cells. These findings suggest biglycan may serve as a potential target in preventing NO-induced neurodegenerative diseases.
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University No 601 Jinsui Road, Xinxiang 453003, Henan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University No 601 Jinsui Road, Xinxiang 453003, Henan, China.
authors.forename: Bin-Feng
authors.initials: BF
authors.lastname: Cheng
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University No 601 Jinsui Road, Xinxiang 453003, Henan, China.
authors.forename: Hai-Jie
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University No 601 Jinsui Road, Xinxiang 453003, Henan, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Xinxiang Medical University No 601 Jinsui Road, Xinxiang 453003, Henan, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Feng
keywords: Nuclear factor-kappaB; apoptosis; biglycan; neuroblastoma; nitric oxide