title: Expression of Vascular Endothelial Growth Factor Has a Regulatory Role in Gingival Venules in Experimental Diabetes.
abstract: BACKGROUND
  In the present study, the possible localization and role of vascular endothelial growth factor receptor type 2 (VEGFR2) in the regulation of gingival venules in a rat model of experimental diabetes are examined.
  
  
  METHODS
  Six weeks after streptozotocin premedication, Wistar male rats presenting blood sugar levels >20 mmol/L were selected for investigation. The VEGFR2 antagonist ZM323881 [5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro-2-methylphenol-hydrochloride] (20 μg/mL) was dripped onto the gingiva between the mandibular incisors. Changes in diameter of the selected gingival venule were measured by vital microscopy combined with digital photography at specified times. Immunohistochemical staining was used to localize VEGFR2. For controls, the same protocol was used on animals with normal blood sugar levels and healthy gingiva.
  
  
  RESULTS
  There was a significant difference between the baseline venule diameter of the diabetic and the control groups (47 ± 1 and 28 ± 2 μm, respectively). After 15, 30, and 60 minutes of local application of ZM323881, significant vasoconstriction was observed in the venules of diabetic rats compared with the baseline (81.4% ± 4.6%, 81.8% ± 4.4%, and 80.6% ± 5.1%, respectively). The control group showed no change in the venule diameter. The immunohistochemical analysis showed significantly increased VEGFR2 expression in the mast cells along the venules in the diabetic group, whereas mast cells were rarely found in the control group.
  
  
  CONCLUSIONS
  The findings suggest that VEGF expression is increased in gingiva in experimentally induced diabetes. After VEGFR2 activation, the mast cell-derived vasodilatory and inflammatory mediators may contribute markedly to the concomitant changes in the microcirculation.
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Budapest, Hungary.|Department of Preventive, Restorative, and Pediatric Dentistry; School of Dental Medicine; University of Bern; Bern, Switzerland.
authors.forename: Milán
authors.initials: M
authors.lastname: Gyurkovics
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Budapest, Hungary.
authors.forename: Izabella
authors.initials: I
authors.lastname: Nagy
authors.affiliation: Paediatrics and Nephrology Research Group of the Hungarian Academy of Sciences and Semmelweis University, Institute of Pathophysiology, Faculty of Medicine, Semmelweis University.
authors.forename: Csaba
authors.initials: C
authors.lastname: Bodor
authors.affiliation: Department of Anatomy, Histology, and Embryology; Faculty of Medicine; Semmelweis University.
authors.forename: Andrea D
authors.initials: AD
authors.lastname: Székely
authors.affiliation: Institute of Health Informatics Development and Further Training, Faculty of Medicine, Semmelweis University.
authors.forename: Elek
authors.initials: E
authors.lastname: Dinya
authors.affiliation: Paediatrics and Nephrology Research Group of the Hungarian Academy of Sciences and Semmelweis University, Institute of Pathophysiology, Faculty of Medicine, Semmelweis University.
authors.forename: László
authors.initials: L
authors.lastname: Rosivall
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, Budapest, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Lohinai
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D005881:Gingiva; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A; D014699:Venules
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Diabetes mellitus; gingiva; inflammation; mast cells; vascular endothelial growth factor receptor-2 antagonist; vasodilation