title: Diagnostic and prognostic value of tissue and circulating levels of Ephrin-A2 in prostate cancer.
abstract: Ephrin-A2, a member of the Eph/ephrin family, is associated with tumorigenesis and tumor progression. This study aimed to assess the diagnostic and prognostic value of both serum and tissue levels of Ephrin-A2 in prostate cancer (PCa) management. One hundred and forty-five frozen prostate tissues, 55 paraffin-embedded prostate tissues, 88 serum samples, and seven prostate cell lines (RWPE-1, LNCaP, LNCaP-LN3, PC-3, PC-3M, PC-3M-LN4, and DU145) were examined via quantitative reverse transcription-PCR (qRT-PCR), immunohistochemistry, enzyme-linked immunosorbent assay, and western blotting. Induced Ephrin-A2 messenger RNA (mRNA) or protein expression was detected in 8.6 % (5/58) benign prostatic hyperplasia (BPH), 59.8 % (52/87) PCa, and five prostate cancer cell lines. Ephrin-A2 immunostaining was present in 6.7 % (1/15) patients with BPHs and 62.5 % (25/40) clinically localized PCa. Accordingly, serum Ephrin-A2 was significantly higher in PCa patients compared to those in the BPH patients and controls (P < 0.001). The expression of Ephrin-A2 was higher in tumor patients with an elevated Gleason score or T3-T4 staging. Ephrin-A2 expression was correlated with Ki-67 expression in PCa patients, both at the gene scale and protein level. Our data indicate that Ephrin-A2 is a potential diagnostic and prognostic biomarker and a promising molecular therapeutic target to attenuate prostate cancer progression.
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.|Department of Laboratory Medicine, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Shibao
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital North, Fudan University, Shanghai, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Kang
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: He
authors.affiliation: Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Xinju
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.|Department of Laboratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
authors.forename: Qunfeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China.|Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, 12 Central Urumqi Road, Shanghai, China. guanming88@yahoo.com.|Department of Laboratory Medicine, Huashan Hospital North, Fudan University, Shanghai, China. guanming88@yahoo.com.|Central Laboratory, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China. guanming88@yahoo.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Guan
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D036383:Ephrin-A2 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008297:Male; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D011379:Prognosis; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D036383:Ephrin-A2; D019394:Ki-67 Antigen
keywords: Ephrin-A2; Gleason score; Ki-67; Prostate cancer; TNM staging