title: Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats.
abstract: OBJECTIVE
  We investigated the effffects and the possible mechanism of microinjection of histamine into cerebellar fastigial nucleus (FN) on stress-induced gastric mucosal damage (SGMD) in rats. The effect of microinjection of histamine into FN on SGMD was observed.
  
  
  METHODS
  The model of SGMD was established by restraint and water (21 ± 1°C)-immersion (RWI) for 3 h in rats. The gastric mucosal damage index indicated the severity of SGMD. Western blotting was performed to assess gastric mucosal cell apoptosis and proliferation.
  
  
  RESULTS
  We observed that histamine microinjection into the FN markedly attenuated SGMD in a dose-dependent manner, and was prevented by pre-treatment with the ranitidine (a selective histamine H2 receptor antagonist) into the FN. The effect of histamine was abolished by pre-treatment with 3-MPA (a glutamic acid decarboxylase antagonist) into the FN. There was a decrease in the discharge frequency of greater splanchnic nerve, and an increase in gastric mucosal blood flow after histamine injection into the FN. Additionally, anti-apoptotic and anti-oxidative factors of gastric mucosa might be involved in this process.
  
  
  CONCLUSIONS
  The exogenous histamine in FN participates in the regulation of SGMD, and our results may help to provide new ideas on the treatment of gastroenterological diseases.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China ; Department of Gastroenterology, The Affiliated Huai'an Hospital of Xuzhou Medical College 62 South Huaihai Road, Huai'an, 223002, Jiangsu, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Stem Cell Engineering Research Center, School of Medicine, Anhui University of Science & Technology Huainan 232001, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China.
authors.forename: Jianfu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College Huai'an 223002, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.affiliation: Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College Huai'an 223002, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, The Affiliated Huai'an Hospital of Xuzhou Medical College 62 South Huaihai Road, Huai'an, 223002, Jiangsu, China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China.
authors.forename: Sujuan
authors.initials: S
authors.lastname: Fei
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China.
authors.forename: Jinzhou
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China.
authors.forename: Shengping
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China.
authors.forename: Zhangbo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Xuzhou Medical College 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China ; Department of Physiology, Xuzhou Medical College 84 West Huaihai Road, Xuzhou, 221002 Jiangsu, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The Affiliated Huai'an Hospital of Xuzhou Medical College 62 South Huaihai Road, Huai'an, 223002, Jiangsu, China.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Lv
authors.affiliation: Clinical Laboratory, The Affiliated Huai'an Hospital of Xuzhou Medical College Huai'an 223002, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
keywords: Histamine; decussation of superior cerebellar peduncle; fastigial nucleus; lateral hypothalamic area; stress-induced gastric mucosal damage