title: Chitosan-based hydrogel implants enriched with calcium ions intended for peripheral nervous tissue regeneration.
abstract: A new method for fabrication of chitosan-based hydrogel implants intended for peripheral nervous tissue regeneration was developed. The method is based on an electrodeposition phenomenon from a solution of chitosan and organic acid. In order to increase the mechanical strength of the implant, the solution was enriched with hydroxyapatite. Hydroxyapatite served as a source of calcium ions too. The influence of the concentration of the polymer and the additive on chemical, mechanical as well as biological properties of the obtained implant was evaluated. The study showed great dependence of the initial solution composition mainly on the physicochemical properties of the resulting structure. Basic in vitro cytotoxic and pro-inflammatory assays showed biocompatibility of manufactured implants, therefore, animal experimentations may be considered.
authors.affiliation: Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland. Electronic address: katarzyna.nawrotek@p.lodz.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Nawrotek
authors.affiliation: Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland. Electronic address: michal.tylman@p.lodz.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Tylman
authors.affiliation: Department of Immunology and Infectious Biology, The Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16 Street, 90-237 Lodz, Poland. Electronic address: rudnicka@biol.uni.lodz.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Rudnicka
authors.affiliation: Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland. Electronic address: jacek.balcerzak@p.lodz.pl.
authors.forename: Jacek
authors.initials: J
authors.lastname: Balcerzak
authors.affiliation: Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland. Electronic address: kamil.kaminski@p.lodz.pl.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kamiński
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000009:adverse effects / Q000737:chemistry*; D051379:Mice; D009416:Nerve Regeneration; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D048271:Chitosan; D002118:Calcium
keywords: Biomaterials; Chitosan; Electrodeposition; Hydrogel; Nerve regeneration