title: Sesamin attenuates mast cell-mediated allergic responses by suppressing the activation of p38 and nuclear factor-κB.
abstract: Establishing therapeutic agents for the treatment of allergic diseases is an important focus of human health research. Sesamin, a lignan in sesame oil, exhibits a diverse range of pharmacological properties. However, to the best of our knowledge, the effect of sesamin on mast cell‑mediated allergic responses has not yet been investigated. Thus, the aim of the present study was to investigate the effect of sesamin on mast cell‑mediated allergic responses and the underlying mechanisms by which it produces this effect. In rats, oral administration of sesamin inhibited passive cutaneous anaphylaxis. Sesamin exposure attenuated immunoglobulin E‑induced histamine release from rat peritoneal mast cells, which was indicated to be mediated by the modulation of intracellular calcium. In human mast cells, sesamin reduced the stimulatory effects of phorbol 12‑myristate 13‑acetate and calcium ionophore A23187 on the production and secretion of pro‑inflammatory cytokines, including tumor necrosis factor‑α and interleukin‑6. The inhibitory effect of sesamin on pro‑inflammatory cytokine production was dependent on nuclear factor κ‑light‑chain‑enhancer of activated B cells (NF‑κB) and p38 mitogen‑activated protein kinase (MAPK). The present study demonstrates that sesamin inhibits mast cell‑derived inflammatory allergic reactions by blocking histamine release, and pro‑inflammatory cytokine production and secretion. In addition, the findings indicate that the effect of sesamin is mediated by its effect on p38 MAPK/NF‑κB signaling. Furthermore, the in vivo and in vitro anti‑allergic effects of sesamin reported in the present study suggest that it is a promising therapeutic agent for the treatment of inflammatory allergic diseases.
authors.affiliation: Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, Jilin 133002, P.R. China.
authors.forename: Liang Chang
authors.initials: LC
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, Yanbian University, Yanji, Jilin 133002, P.R. China.
authors.forename: Hong Mei
authors.initials: HM
authors.lastname: Piao
authors.affiliation: College of Pharmacy, Yanbian University, Yanji, Jilin 133002, P.R. China.
authors.forename: Ming Yu
authors.initials: MY
authors.lastname: Zheng
authors.affiliation: Department of Pathology, Yanbian University School of Basic Medical Sciences, Yanji, Jilin 133000, P.R. China.
authors.forename: Zhen Hua
authors.initials: ZH
authors.lastname: Lin
authors.affiliation: Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, Jilin 133002, P.R. China.
authors.forename: Guangzhao
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Anatomy, Histology and Embryology, Yanbian University Medical College, Yanji, Jilin 133002, P.R. China.
authors.forename: Guang Hai
authors.initials: GH
authors.lastname: Yan
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004149:Dioxoles / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism*; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010537:Peritoneum / Q000166:cytology; D017207:Rats, Sprague-Dawley; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004149:Dioxoles; D018836:Inflammation Mediators; D017705:Lignans; D016328:NF-kappa B; D007073:Immunoglobulin E; D048051:p38 Mitogen-Activated Protein Kinases; D019297:2,4-Dinitrophenol; C054125:sesamin; D002118:Calcium