title: Digestibility and prebiotic properties of potato rhamnogalacturonan I polysaccharide and its galactose-rich oligosaccharides/oligomers.
abstract: Galactose-rich oligosaccharides/oligomers (oligo-RG I) were produced by the enzymatic treatment of potato galactan-rich rhamnogalacturonan I (RG I) with endo-β-1,4-galactanase and Depol 670L multi-enzymatic preparation. The digestibility study revealed that 81.6 and 79.3% of RG I and its corresponding oligomers remained unhydrolyzed, respectively. The prebiotic properties of RG I and its hydrolysates were investigated using a continuous culture system inoculated with immobilized fecal microbiota. Both RG I and oligo-RG I have stimulated the growth of Bifidobacterium spp. and Lactobacillus spp., with oligo-RG I hydrolysates being more selectively fermented by these beneficial bacteria. Furthermore, none of RG I nor its hydrolysates increased the populations of Bacteroidetes and Clostridium leptum. Total amounts of short chain fatty acids, generated upon the fermentation of oligo-RG I, were higher than those obtained with its parent RG I and the positive control (fructooligosaccharides). The overall study contributes to the understandings of the prebiotic properties of potato RG I and its corresponding oligosaccharides/oligomers.
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, H9X 3V9 QC, Canada.
authors.forename: Nastaran
authors.initials: N
authors.lastname: Khodaei
authors.affiliation: Nutrition and Functional Foods Institute, Université Laval, G1V 0A6 QC, Canada.
authors.forename: Benoit
authors.initials: B
authors.lastname: Fernandez
authors.affiliation: Nutrition and Functional Foods Institute, Université Laval, G1V 0A6 QC, Canada.
authors.forename: Ismail
authors.initials: I
authors.lastname: Fliss
authors.affiliation: Department of Food Science and Agricultural Chemistry, McGill University, H9X 3V9 QC, Canada; Nutrition and Functional Foods Institute, Université Laval, G1V 0A6 QC, Canada. Electronic address: salwa.karboune@mcgill.ca.
authors.forename: Salwa
authors.initials: S
authors.lastname: Karboune
mesh_terms: D041963:Bacteroidetes / Q000302:isolation & purification / Q000378:metabolism; D019149:Bioreactors; D003013:Clostridium / Q000302:isolation & purification / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005690:Galactose / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D007778:Lactobacillus / Q000302:isolation & purification / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D010368:Pectins / Q000737:chemistry / Q000378:metabolism*; D056692:Prebiotics; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D009844:Oligosaccharides; D056692:Prebiotics; C042491:rhamnogalacturonan I; D010368:Pectins; D005690:Galactose
keywords: Digestibility; Potato; Prebiotic; Rhamnogalacturonan I; Short chain fatty acid