title: A targeted proteomic strategy for the measurement of oral cancer candidate biomarkers in human saliva.
abstract: Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancy in the world and are characterized by poor prognosis and a low survival rate. Saliva is oral fluid with intimate contact with OSCC. Besides non-invasive, simple, and rapid to collect, saliva is a potential source of biomarkers. In this study, we build an SRM assay that targets fourteen OSCC candidate biomarker proteins, which were evaluated in a set of clinically-derived saliva samples. Using Skyline software package, we demonstrated a statistically significant higher abundance of the C1R, LCN2, SLPI, FAM49B, TAGLN2, CFB, C3, C4B, LRG1, SERPINA1 candidate biomarkers in the saliva of OSCC patients. Furthermore, our study also demonstrated that CFB, C3, C4B, SERPINA1 and LRG1 are associated with the risk of developing OSCC. Overall, this study successfully used targeted proteomics to measure in saliva a panel of biomarker candidates for OSCC.
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, Brazil.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Kawahara
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Bollinger
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, Brazil.
authors.forename: César
authors.initials: C
authors.lastname: Rivera
authors.affiliation: Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, São Paulo, Brazil.
authors.forename: Ana Carolina P
authors.initials: AC
authors.lastname: Ribeiro
authors.affiliation: Instituto do Câncer do Estado de São Paulo, Octavio Frias de Oliveira, São Paulo, Brazil.
authors.forename: Thaís Bianca
authors.initials: TB
authors.lastname: Brandão
authors.affiliation: Laboratório de Espectrometria de Massas, Laboratório Nacional de Biociências, LNBio, CNPEM, Campinas, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.affiliation: Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009055:Mouth / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012463:Saliva / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins
keywords: Biomedicine; Oral cancer; Saliva; Selected reaction monitoring; Skyline