title: Fatty acid profiles of four filamentous green algae under varying culture conditions.
abstract: Although benthic filamentous algae are interesting targets for wastewater treatment and biotechnology, relatively little is known about their biochemical composition and variation in response to growth conditions. Fatty acid composition of four benthic filamentous green algae was determined in different culture conditions. Although the response was partly species-dependent, increasing culture age, nitrogen deprivation and dark exposure of stationary phase greatly increased both total fatty acid content (TFA) from 12-35 to 40-173mgg(-1) dry weight (DW) and the relative proportion of polyunsaturated fatty acids (PUFAs) from 21-58% to 55-87% of TFA, with dark exposure having the greatest effect. However, the main variation in fatty acid composition was between species, with Uronema being rich in C16:0 (2.3% of DW), Klebsormidium in C18:2ω6 (5.4% of DW) and Stigeoclonium in C18:3ω3 (11.1% of DW). This indicates the potential of the latter two species as potential sources of these PUFAs.
authors.affiliation: Laboratory of Protistology and Aquatic Ecology, Department of Biology, Ghent University, Krijgslaan 281-S8, 9000 Ghent, Belgium; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, 71 East Beijing Road, Nanjing 210008, China. Electronic address: liujzh04@aliyun.com.
authors.forename: Junzhuo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Laboratory of Protistology and Aquatic Ecology, Department of Biology, Ghent University, Krijgslaan 281-S8, 9000 Ghent, Belgium.
authors.forename: Pieter
authors.initials: P
authors.lastname: Vanormelingen
authors.affiliation: Laboratory of Protistology and Aquatic Ecology, Department of Biology, Ghent University, Krijgslaan 281-S8, 9000 Ghent, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Vyverman
mesh_terms: D017756:Aquaculture / Q000379:methods*; D001709:Biotechnology / Q000379:methods; D000460:Chlorophyta / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D013045:Species Specificity
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009584:Nitrogen
keywords: Dark exposure; Filamentous green algae; Growth phase; Nitrogen deprivation; Polyunsaturated fatty acid