title: Assessing Kinase Activity in Plants with In-Gel Kinase Assays.
abstract: The in-gel protein kinase assay is a powerful method to measure the protein phosphorylation activity of specific protein kinases. Any protein substrate can be embedded in polyacrylamide gels where they can be phosphorylated by protein kinases that are separated in the gel under denaturing conditions and then renatured. The kinase activity can be visualized in situ in the gels by autoradiography. This method has been used to compare the activities of protein kinases in parallel samples or to identify their potential substrates. Here, we describe in detail an in-gel kinase assay to measure the activity of some protein kinases in plants.
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, IN, 47907, USA.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, IN, 47907, USA. jkzhu@purdue.edu.|Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Shanghai, 200032, China. jkzhu@purdue.edu.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
mesh_terms: D057075:Enzyme Assays / Q000379:methods*; D005782:Gels; D010940:Plant Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*
chemical_list: D005782:Gels; D010940:Plant Proteins; D011494:Protein Kinases
keywords: Autoradiography; CDPK; Casein kinase; MAPK; Protein kinase; SnRK2; Substrate