title: Exogenous hTERT gene transfected endothelial progenitor cells from bone marrow promoted angiogenesis in ischemic myocardium of rats.
abstract: OBJECTIVE
  To explore the biological behavior and the revascularizative ability of endothelial progenitor cells (EPCs) transfected with human telomerase reverse transcriptase (hTERT) gene.
  
  
  METHODS
  EPCs were isolated from mononuclear cells in bone marrow by using the method of density gradient centrifugation, then cultured with differential velocity adherent method, EPCs were transfected by recombinant plasmid carrying GFP report gene EGFP-hTERT. The EPCs secretion and proliferation ability were detected before and after transfection. The expression of EPCs mRNA were detected by RT-PCR before and after transfection. The new capillaries of infarct area were observed.
  
  
  RESULTS
  After transgenesis, the proliferation of EPCs were increased, and the secretion of NO, LDH, iNOS by EPCs were significantly increased compared to the non-transgenesis group. After transplanted the transfected EPCs into the ischemic myocardial of rats, revascularization were increased obviously.
  
  
  CONCLUSIONS
  EPCs maintained the original biological characteristics after transfecting exogenous hTER gene, the proliferation and survival rate were up-regulated significantly, and the revascularization ability of EPCs were significantly strengthen.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong, China.
authors.forename: Shang-Hai
authors.initials: SH
authors.lastname: Li
authors.affiliation: Department of Cardiology, Central Hospital of Xiaogan Xiaogan, Wuhan, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong, China.
authors.forename: Yun-Jun
authors.initials: YJ
authors.lastname: Xu
authors.affiliation: Department of Cardiology, Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong, China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Ma
authors.affiliation: Department of Cardiology, Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong, China.
authors.forename: Xing-Yue
authors.initials: XY
authors.lastname: Li
authors.affiliation: Department of Cardiology, Affiliated Hospital of Guangdong Medical College Zhanjiang, Guangdong, China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Liang
keywords: Endothelial progenitor cells; human telomerase reverse transcriptase (hTERT); myocardial infarction; transfection