title: Effects of human Dachshund homolog 1 on the proliferation, migration, and adhesion of squamous cell carcinoma of the tongue.
abstract: OBJECTIVE
  To investigate the expression and role of human Dachshund homolog 1 (DACH1) in the tongue squamous cell carcinoma (TSCC).
  
  
  METHODS
  To explore the expression, regulation, and mechanism of DACH1 in TSCC, nine samples of fresh tumor and adjacent tissues, 51 samples of paraffin-embedded TSCC and paired adjacent tissues, and TSCC cell line SCC-25 were examined. Immunohistochemistry, real-time polymerase chain reaction, Western blot, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, Transwell, adhesion assays, and flow cytometry were used.
  
  
  RESULTS
  The DACH1 expression level was significantly lower in tumors than in the adjacent tissues, and such low expression was associated with poor differentiation of tumors, late clinical stage, and lymph node metastasis. Moreover, overexpression of DACH1 might promote apoptosis and inhibit the proliferation, migration, and adhesion of SCC-25 cells.
  
  
  CONCLUSIONS
  DACH1 may be a potential molecular target for the therapy of recurrent and metastatic TSCC.
authors.affiliation: Professor/Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Professor/Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China; Professor/Attending Physician, Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China. Electronic address: Wangcq1121@126.com.
authors.forename: Cheng-Qin
authors.initials: CQ
authors.lastname: Wang
authors.affiliation: Professor/Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Liu
authors.affiliation: Professor, Department of Stomatology, Qilu Hospital, and Institute of Stomatology, Shandong University, Jinan, Shandong, China.
authors.forename: Zuo-Qing
authors.initials: ZQ
authors.lastname: Dong
authors.affiliation: Professor/Attending Physician, Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Professor/Attending Physician, Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China.
authors.forename: Xian-Ning
authors.initials: XN
authors.lastname: Dong
authors.affiliation: Professor, Department of Stomatology, Qilu Hospital, and Institute of Stomatology, Shandong University, Jinan, Shandong, China.
authors.forename: Fengcai
authors.initials: F
authors.lastname: Wei
authors.affiliation: Professor, Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Qu
authors.affiliation: Professor/Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China; Professor/Attending Physician, Department of Pathology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China. Electronic address: Xiangfenggang@163.com.
authors.forename: Feng-Gang
authors.initials: FG
authors.lastname: Xiang
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: C486747:DACH1 protein, human; D005136:Eye Proteins; D014157:Transcription Factors