title: Reveromycin A Administration Prevents Alveolar Bone Loss in Osteoprotegerin Knockout Mice with Periodontal Disease.
abstract: Chronic periodontal disease is characterized by alveolar bone loss and inflammatory changes. Reveromycin A (RMA) was recently developed and is a unique agent for inhibiting osteoclast activity. This study analysed the effects of RMA in an experimental mouse model of periodontitis involving osteoprotegerin (OPG)-knockout mice, specifically, whether it could control osteoclasts and reduce inflammation in periodontal tissue. We examined wild-type (WT) and OPG knockout mice (OPG KO) ligated with wire around contact points on the left first and second molars. RMA was administered twice a day to half of the mice. Using micro-computed tomography, we measured the volume of alveolar bone loss between the first and second molars, and also performed histological analysis. The OPG KO RMA+ group had significantly decreased osteoclast counts, alveolar bone loss, attachment loss, and inflammatory cytokine expression 8 weeks after ligation. Thus, RMA may reduce alveolar bone loss and inflamed periodontal tissues in patients with periodontitis.
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Mizuno
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Miyazawa
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Tabuchi
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yoshizako
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Chisato
authors.initials: C
authors.lastname: Minamoto
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Torii
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tamaoka
authors.affiliation: Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama 351-0198, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawatani
authors.affiliation: Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama 351-0198, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.affiliation: Department of Oral Pathology, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8650, Japan.
authors.forename: Hatsuhiko
authors.initials: H
authors.lastname: Maeda
authors.affiliation: Department of Orthodontics, School of Dentistry, Aichi Gakuin University, Nagoya, Aichi 464-8651, Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Goto
mesh_terms: D016301:Alveolar Bone Loss / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D053244:Osteoprotegerin / Q000172:deficiency*; D010510:Periodontal Diseases / Q000150:complications* / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011714:Pyrans / Q000494:pharmacology*; D013141:Spiro Compounds / Q000494:pharmacology*; D055114:X-Ray Microtomography
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053244:Osteoprotegerin; D011714:Pyrans; D013141:Spiro Compounds; C068098:reveromycin A