title: Activation of Mitochondrial Uncoupling Protein 4 and ATP-Sensitive Potassium Channel Cumulatively Decreases Superoxide Production in Insect Mitochondria.
abstract: It has been evidenced that mitochondrial uncoupling protein 4 (UCP4) and ATP-regulated potassium channel (mKATP channel) of insect Gromphadorhina coqereliana mitochondria decrease superoxide anion production. We elucidated whether the two energy-dissipating systems work together on a modulation of superoxide level in cockroach mitochondria. Our data show that the simultaneous activation of UCP4 by palmitic acid and mKATP channel by pinacidil revealed a cumulative effect on weakening mitochondrial superoxide formation. The inhibition of UCP4 by GTP (and/or ATP) and mKATP channel by ATP elevated superoxide production. These results suggest a functional cooperation of both energy-dissipating systems in protection against oxidative stress in insects.
authors.affiliation: Department of Animal Physiology and Development, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland. slocina@amu.edu.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Slocińska
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Rosinski
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Jarmuszkiewicz
mesh_terms: D000818:Animals; D003058:Cockroaches / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D019308:Palmitic Acid / Q000494:pharmacology; D020110:Pinacidil / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D054086:KATP Channels; D026901:Membrane Transport Proteins; D013481:Superoxides; D019308:Palmitic Acid; D020110:Pinacidil