title: Reproducibility of Neonate Ocular Circulation Measurements Using Laser Speckle Flowgraphy.
abstract: Measuring the ocular blood flow in neonates may clarify the relationships between eye diseases and ocular circulation abnormalities. However, no method for noninvasively measuring ocular circulation in neonates is established. We used laser speckle flowgraphy (LSFG) modified for neonates to measure their ocular circulation and investigated whether this method is reproducible. During their normal sleep, we studied 16 subjects (adjusted age of 34-48 weeks) whose blood flow could be measured three consecutive times. While the subjects slept in the supine position, three mean blur rate (MBR) values of the optic nerve head (ONH) were obtained: the MBR-A (mean of all values), MBR-V (vessel mean), and MBR-T (tissue mean), and nine blood flow pulse waveform parameters in the ONH were examined. We analyzed the coefficient of variation (COV) and the intraclass correlation coefficient (ICC) for each parameter. The COVs of the MBR values were all ≤ 10%. The ICCs of the MBR values were all >0.8. Good COVs were observed for the blowout score, blowout time, rising rate, falling rate, and acceleration time index. Although the measurement of ocular circulation in the neonates was difficult, our results exhibited reproducibility, suggesting that this method could be used in clinical research.
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Itokawa
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Shiba
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Katayama
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Arimura
authors.affiliation: Department of Neonatology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Mizukaki
authors.affiliation: Department of Neonatology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yoda
authors.affiliation: Department of Ophthalmology, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hori
mesh_terms: D000328:Adult; D001783:Blood Flow Velocity / Q000502:physiology; D005260:Female; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods; D007834:Lasers; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D008875:Middle Aged; D009898:Optic Disk / Q000098:blood supply*; D012039:Regional Blood Flow / Q000502:physiology*; D015203:Reproducibility of Results