title: Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas.
abstract: Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and used to test adults of both laboratory and field populations. Larval susceptibility was also tested using both laboratory and field populations. Significant differences were recorded in adult and larval susceptibility among WCR field and laboratory populations. The highest LC50 for WCR adults was observed in populations from Keith 2 and Chase Counties, NE, with LC50s of 2.2 and 1.38 μg/vial, respectively, and Finney County 1, KS, with 1.43 μg/vial, as compared to a laboratory non-diapause population (0.24 μg/vial). For larvae, significant differences between WCR field and laboratory populations were also recorded. Significant differences in mortalities at the diagnostic bifenthrin concentration (LC99) were observed among WCR adult populations with western Corn Belt populations exhibiting lower susceptibility to bifenthrin, especially in southwestern Nebraska and southwestern Kansas. This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides. Implications to sustainable rootworm management are discussed.
authors.affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Adriano E
authors.initials: AE
authors.lastname: Pereira
authors.affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Haichuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Southwest Research and Extension Center, Kansas State University, Garden City, Kansas, United States of America.
authors.forename: Sarah N
authors.initials: SN
authors.lastname: Zukoff
authors.affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Lance J
authors.initials: LJ
authors.lastname: Meinke
authors.affiliation: North Central Agricultural Research Laboratory, United States Department of Agriculture-Agriculture Research Service, Brookings, South Dakota, United States of America.
authors.forename: B Wade
authors.initials: BW
authors.lastname: French
authors.affiliation: Department of Entomology, University of Nebraska-Lincoln, Lincoln, Nebraska, United States of America.
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000187:drug effects* / Q000502:physiology; D007305:Insecticide Resistance; D007306:Insecticides / Q000633:toxicity*; D007615:Kansas; D007814:Larva / Q000187:drug effects / Q000502:physiology; D009329:Nebraska; D011722:Pyrethrins / Q000633:toxicity*; D003313:Zea mays / Q000469:parasitology*
chemical_list: D007306:Insecticides; D011722:Pyrethrins; C099952:bifenthrin