title: A study of the expression and localization of toll-like receptors 2 and 9 in different grades of cervical intraepithelial neoplasia and squamous cell carcinoma.
abstract: TLRs are important molecules of innate immune response, those play central role in host pathogen interaction and recognition through pathogen associated molecular patterns (PAMPs). Previous studies have indicated the role of TLRs in many human malignancy and cervical cancer in terms of viral recognition and inflammatory changes in-vivo. The objective of this study was to evaluate the expression and localization of toll-like receptor (TLR) 2 and TLR9 in preinvasive and invasive cervical cancer patients and to investigate its use as a probable diagnostic tool for better management cervical cancer. This single institution study includes individuals with normal, precancerous lesions, cervical intraepithelial neoplastic (CIN) and invasive squamous cell carcinoma (SCC) of the cervix. Upon confirmation by histopathology, fluorescence based immunohistochemistry was performed in all patients for TLR2 and TLR9, followed by semi-quantitative estimation of the staining intensity and grade of expression. The expression pattern of TLR2 and TLR9 does not vary greatly from normal to precancerous lesions, but a significant variation was observed in advance stages, i.e. squamous cell carcinoma of the uterine cervix. Additionally the expression increased marginally in higher grades. In spite of their low difference in expression along different stages of cervical cancer, both TLR2 and TLR9 could detect the disease at an advance stages as depicted by the receiver operator characteristics curve analysis.
authors.affiliation: Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, West Bengal, India.
authors.forename: Arnab
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Department of Biochemistry, Calcutta National Medical College, Kolkata, India.
authors.forename: Anindya
authors.initials: A
authors.lastname: Dasgupta
authors.affiliation: Department of Pathology, Burdwan Medical College and Hospital, Burdwan, India.
authors.forename: Arghya
authors.initials: A
authors.lastname: Bandyopadhyay
authors.affiliation: Department of Pathology, Bankura Medical College and Hospital, Bankura, India.
authors.forename: TapanKumar
authors.initials: T
authors.lastname: Ghosh
authors.affiliation: Department of Gynecology and Obstetrics, Burdwan Medical College and Hospital, Burdwan, India.
authors.forename: Rabindranath
authors.initials: R
authors.lastname: Dalui
authors.affiliation: Department of Gynecology and Obstetrics, Burdwan Medical College and Hospital, Burdwan, India.
authors.forename: Subhas
authors.initials: S
authors.lastname: Biswas
authors.affiliation: Department of Pathology, Burdwan Medical College and Hospital, Burdwan, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Banerjee
authors.affiliation: Molecular Biology and Human Genetics Laboratory, Department of Zoology, The University of Burdwan, West Bengal, India. Electronic address: abasu@zoo.buruniv.ac.in.
authors.forename: Anupam
authors.initials: A
authors.lastname: Basu
mesh_terms: D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D060787:Neoplasm Grading; D012372:ROC Curve; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis*; D051217:Toll-Like Receptor 9 / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*; D002578:Uterine Cervical Dysplasia / Q000378:metabolism / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C495342:TLR2 protein, human; C495371:TLR9 protein, human; D051195:Toll-Like Receptor 2; D051217:Toll-Like Receptor 9
keywords: Cervical intraepithelial neoplasia; Invasive squamous cell carcinoma; Toll like receptor