title: Comparative study of collagen deposition in the colon wall of patients operated for sigmoid diverticular disease.
abstract: OBJECTIVE
  To investigate the deposition of collagen in the colon wall of patients with sigmoid diverticulitis.
  
  
  METHODS
  Samples of sigmoid tissue from 15 patients (disease group), seven men and eight women aged 37-77 years who underwent surgery for the treatment of diverticulitis, were selected. For the control group, specimens from five patients, three men and two women aged 19-58 years undergoing emergency surgery for sigmoid trauma were selected. These subjects had no associated diseases. The histological study of the surgical specimens was performed by staining with hematoxylin-eosin and picrosirius and using a histochemical method for collagen quantification.
  
  
  RESULTS
  Collagen deposition in the colon wall in terms of area (F), glandular epithelium (E) and total area was significantly higher in the disease group compared to control (p=0.003, p=0.026 and p=0.010, respectively). The collagen volume fraction (F fraction) and muscle tissue (M fraction) were also significantly higher compared to control (p=0.044 and p=0.026, respectively). The muscle (M area) and volume fraction of glandular epithelium (E fraction) did not differ significantly between the two groups, (p=0.074 and p=1.000, respectively).
  
  
  CONCLUSIONS
  In this study, collagen deposition in the colon wall of the patients operated for sigmoid diverticulitis was higher compared to patients without the disease.
authors.affiliation: Department of Surgery, Medical School of Jundiai, Jundiai, SP, Brazil.
authors.forename: Mário
authors.initials: M
authors.lastname: Pantaroto
authors.affiliation: Department of Gastroenterology, Universidade Federal de São Paulo, Brazil.
authors.forename: Gaspar de Jesus
authors.initials: Gde J
authors.lastname: Lopes Filho
authors.affiliation: Department of Pathology, Medical School of Jundiai, Jundiai, SP, Brazil.
authors.forename: Clovis Antonio Lopes
authors.initials: CA
authors.lastname: Pinto
authors.affiliation: Department of Pathology, Medical School of Jundiai, Jundiai, SP, Brazil.
authors.forename: Armando
authors.initials: A
authors.lastname: Antico Filho
mesh_terms: D000328:Adult; D000368:Aged; D003094:Collagen / Q000032:analysis / Q000378:metabolism*; D012809:Colon, Sigmoid / Q000378:metabolism* / Q000601:surgery*; D004239:Diverticulitis, Colonic / Q000601:surgery*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000378:metabolism; D012016:Reference Values; D012810:Sigmoid Diseases / Q000601:surgery*; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D003094:Collagen