title: Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae.
abstract: Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.
authors.affiliation: Arslanbey Vocational School, Kocaeli University, Kocaeli, Turkey.|Department of Biotechnology, Institute of Natural and Applied Sciences, Middle East Technical University, Ankara, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Ozer Uyar
authors.affiliation: Department of Biotechnology, Institute of Natural and Applied Sciences, Middle East Technical University, Ankara, Turkey.|Department of Biological Sciences, Faculty of Arts and Sciences, Middle East Technical University, Ankara, Turkey.
authors.forename: Meral
authors.initials: M
authors.lastname: Yücel
authors.affiliation: Department of Biotechnology, Institute of Natural and Applied Sciences, Middle East Technical University, Ankara, Turkey.|Department of Food Engineering, Faculty of Engineering, Middle East Technical University, Ankara, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Hamamcı
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D012233:Rhizopus / Q000201:enzymology* / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014199:Trehalose / Q000096:biosynthesis*
chemical_list: D018076:DNA, Complementary; D004271:DNA, Fungal; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase
keywords: Cloning; Rhizopus oryzae; Trehalose; Trehalose-6-phosphate synthase