title: A fluorogenic substrate of beta-lactamases and its potential as a probe to detect the bacteria resistant to the third-generation oxyimino-cephalosporins.
abstract: We devised and synthesized a fluorogenic substrate of β-lactamases as a probe to detect the activity of the enzymes. Fluorescence of the probe emitted upon treatment of a β-lactamase and increased proportionally to the concentration of the enzyme, demonstrating its sensing property for the activity of the enzyme. We also showed that the probe could be utilized to assay the enzyme and to determine kinetic parameters of the enzyme. Moreover, the probe was able to detect resistance to the third-generation oxyimino-cephalosporin-derived antibiotics such as cefotaxime and ceftazidime. In particular, the probe could identify the ceftazidime-resistance in bacteria that was not detectable using conventional pH-sensing materials, indicating the practical utility of the probe.
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biological Chemistry, KIST campus, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Hien Bao Dieu
authors.initials: HB
authors.lastname: Thai
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Banpo-daero 222 Seocho-gu, Seoul 137-701, Republic of Korea.
authors.forename: Jin Kyung
authors.initials: JK
authors.lastname: Yu
authors.affiliation: Center for Neuro-Medicine, Korea Institute of Science and Technology, Republic of Korea.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Banpo-daero 222 Seocho-gu, Seoul 137-701, Republic of Korea. Electronic address: yjpk@catholic.ac.kr.
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
authors.affiliation: Department of Applied Chemistry, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea. Electronic address: sjmin@hanyang.ac.kr.
authors.forename: Sun-Joon
authors.initials: SJ
authors.lastname: Min
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; Department of Biological Chemistry, KIST campus, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. Electronic address: drahn@kist.re.kr.
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
mesh_terms: D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D015336:Molecular Probe Techniques / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D001618:beta-Lactamases
keywords: Antibiotic resistance; Fluorescence intensity; Fluorogenic probe; Oxyimino-cephalosporins; β-lactamases