title: A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor.
abstract: A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis.
authors.affiliation: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China. maoyu_gt@126.com.|The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. maoyu_gt@126.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China. chenyongli0617@163.com.|The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. chenyongli0617@163.com.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China. lisong@pkusz.edu.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.affiliation: Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, China. lisong@pkusz.edu.cn.|Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA. lisong@pkusz.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.affiliation: The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. jiangyy@sz.tsinghua.edu.cn.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D006108:Graphite; D004247:DNA
keywords: DNA biosensor; Isothermal amplification; aptamer; graphene oxide