title: β-Glucan as an encapsulating agent: Effect on probiotic survival in simulated gastrointestinal tract.
abstract: Three strains of probiotics Lactobacillus casei, Lactobacillus brevis, and Lactobacillus plantarum were encapsulated in β-glucan matrix using emulsion technique. Further the encapsulated cells were studied for their tolerance in simulated gastrointestinal conditions and its storage stability. The average encapsulation efficiency of β-glucan-probiotic beads was found to be 74.01%. The surface morphology of β-glucan containing bacteria was studied using SEM. The noteworthy absorptions in the FT-IR spectra between 1300-900 cm(-1) and 2918-2925 cm(-1) corresponds to the presence of bacteria into the glucan matrix. Also, the thermal stability of β-glucan was evaluated using Differential Scanning Calorimeter. The efficiency of β-glucan in protecting the surviability of probiotic cells under simulated gastrointestinal conditions was studied. Results revealed significant (p<0.05) improvement to tolerance when the encapsulated cells were subjected to stresses like low pH, heat treatment, simulated intestinal conditions and storage.
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 190006, India.
authors.forename: Asima
authors.initials: A
authors.lastname: Shah
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 190006, India. Electronic address: adil.gani@gmail.com.
authors.forename: Adil
authors.initials: A
authors.lastname: Gani
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 190006, India.
authors.forename: Mudasir
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 190006, India.
authors.forename: Bilal Ahmad
authors.initials: BA
authors.lastname: Ashwar
authors.affiliation: Department of Food Science and Technology, University of Kashmir, Srinagar 190006, India.
authors.forename: F A
authors.initials: FA
authors.lastname: Masoodi
mesh_terms: D002152:Calorimetry, Differential Scanning; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000302:isolation & purification; D005750:Gastric Juice; D041981:Gastrointestinal Tract / Q000382:microbiology; D001467:Hordeum / Q000737:chemistry; D006358:Hot Temperature; D050296:Microbial Viability; D019936:Probiotics; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D020099:Coated Materials, Biocompatible; D047071:beta-Glucans
keywords: Encapsulation; FT-IR; β-Glucan