title: Comparative profiling of extractable proteins in extracellular matrices of porcine cholecyst and jejunum intended for preparation of tissue engineering scaffolds.
abstract: Scaffolds prepared from cholecyst and jejunum have differential immunological potential, despite similar biocompatibility, when used as subcutaneous grafts. The reason for differential immunogenicity is probably due to differences in the nature of protein composition and biomolecules in the extracellular matrices (ECMs) of source organs that are used for preparation of the scaffolds. Against this background, the present study aims to identify the extractable proteins of ECMs derived from porcine cholecyst and jejunum. The proteins were extracted and identified through a conventional database search following sodium dodecyl sulfate-polyacrylamide gel-electrophoresis separation and mass spectroscopy. The resultant protein profile was analyzed and at least 154 proteins in cholecyst-derived extracellular matrix (CDE) and 186 proteins in jejunum-derived extracellular matrix (JDE) were identified. Both the matrices contained several extracelluar proteins including fibronectin, nidogen, decorin, and lumican that are known to participate in wound healing responses. However, the CDE had fewer cellular proteins than JDE, especially the latter contained class-I and class-II histocompatibility antigens which are incriminated as potent immunogens responsible for graft rejection. The results of the study suggested that the ECMs used for the scaffold preparation need not be "acellular" and differences in the protein composition of the ECMs might have caused the differential wound healing responses. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 489-496, 2017.
authors.affiliation: Division of Experimental Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: Jaseer
authors.initials: J
authors.lastname: Muhamed
authors.affiliation: Division of Experimental Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: Akhila
authors.initials: A
authors.lastname: Rajan
authors.affiliation: Department of Cardiovascular & Diabetes Disease Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Surendran
authors.affiliation: Department of Cardiovascular & Diabetes Disease Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India.
authors.forename: Abdul
authors.initials: A
authors.lastname: Jaleel
authors.affiliation: Division of Experimental Pathology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
authors.forename: Thapasimuthu V
authors.initials: TV
authors.lastname: Anilkumar
mesh_terms: D000818:Animals; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D005704:Gallbladder / Q000737:chemistry* / Q000378:metabolism; D007421:Intestine, Small / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D016326:Extracellular Matrix Proteins
keywords: cholecyst; extracellular matrix; protein profile; scaffold; xenograft