title: Cassava root membrane proteome reveals activities during storage root maturation.
abstract: Cassava (Manihot esculenta Crantz) is one of the most important crops of Thailand. Its storage roots are used as food, feed, starch production, and be the important source for biofuel and biodegradable plastic production. Despite the importance of cassava storage roots, little is known about the mechanisms involved in their formation. This present study has focused on comparison of the expression profiles of cassava root proteome at various developmental stages using two-dimensional gel electrophoresis and LC-MS/MS. Based on an anatomical study using Toluidine Blue, the secondary growth was confirmed to be essential during the development of cassava storage root. To investigate biochemical processes occurring during storage root maturation, soluble and membrane proteins were isolated from storage roots harvested from 3-, 6-, 9-, and 12-month-old cassava plants. The proteins with differential expression pattern were analysed and identified to be associated with 8 functional groups: protein folding and degradation, energy, metabolism, secondary metabolism, stress response, transport facilitation, cytoskeleton, and unclassified function. The expression profiling of membrane proteins revealed the proteins involved in protein folding and degradation, energy, and cell structure were highly expressed during early stages of development. Integration of these data along with the information available in genome and transcriptome databases is critical to expand knowledge obtained solely from the field of proteomics. Possible role of identified proteins were discussed in relation with the activities during storage root maturation in cassava.
authors.affiliation: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand.
authors.forename: Maliwan
authors.initials: M
authors.lastname: Naconsie
authors.affiliation: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand.
authors.forename: Manassawe
authors.initials: M
authors.lastname: Lertpanyasampatha
authors.affiliation: Deparment of Plant Science, Faculty of Science, Mahidol University, Phayathai, Bangkok, 10400, Thailand.
authors.forename: Unchera
authors.initials: U
authors.lastname: Viboonjun
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), Rangsit, Pathumthani, 10210, Thailand.
authors.forename: Supatcharee
authors.initials: S
authors.lastname: Netrphan
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kuwano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
authors.forename: Naotake
authors.initials: N
authors.lastname: Ogasawara
authors.affiliation: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand. scjnr@mahidol.ac.th.
authors.forename: Jarunya
authors.initials: J
authors.lastname: Narangajavana
mesh_terms: D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D002365:Manihot / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism; D020543:Proteome; D017422:Sequence Analysis, DNA; D053719:Tandem Mass Spectrometry; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Cassava; Membrane protein; Proteomic; Storage root maturation