title: Characterization of a novel purified polysaccharide from the flesh of Cipangopaludina chinensis.
abstract: The purpose of this paper was to investigate the characterization of a novel polysaccharide from the flesh of Cipangopaludina chinensis, named CCPSn. The results found CCPSn was a white powder, readily soluble in hot water and slightly soluble in water. CCPSn was a homopolysaccharide composed of D-glucose (D-Glc) with molecular weight of 91.1 kDa. Based on analysis of UV-visible, FT-IR, periodic acid oxidation, Smith degradation, methylation, GM-MS and NMR, the structure of CCPSn was elucidated as follows: the backbone was composed of (1 → 3) linked α-D-Glc. The branches, consisting of a single (1 → 3) linked α-D-Glc units and terminal α-D-Glc-4-O-SO3(-), were attached to the main chain at C-4 positions. The degree of branching was calculated to be about 16.73%. The C-1 of terminal α-D-Glc-4-O-SO3(-) was linked to O-3 of (1 → 3) linked α-D-Glc in the branches. In addition, the results indicated CCPSn was a sulfated polysaccharide with the sulfate radical content of 9.12%.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, Jiangsu, PR China; School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China. Electronic address: shiyingying82012@163.com.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Shi
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xiong
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Chunhao
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yi
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Chemical Engineering, Huaiyin Institute of Technology, Huaian 223003, Jiangsu, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, Jiangsu, PR China. Electronic address: cuihao@nju.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Cui
mesh_terms: D000818:Animals; D011134:Polysaccharides / Q000737:chemistry*; D012908:Snails / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: Characterization; Cipangopaludina chinensis; Polysaccharide; Purification