title: Cancer Cell Analyses at the Single Cell-Level Using Electroactive Microwell Array Device.
abstract: Circulating tumor cells (CTCs), shed from primary tumors and disseminated into peripheral blood, are playing a major role in metastasis. Even after isolation of CTCs from blood, the target cells are mixed with a population of other cell types. Here, we propose a new method for analyses of cell mixture at the single-cell level using a microfluidic device that contains arrayed electroactive microwells. Dielectrophoretic (DEP) force, induced by the electrodes patterned on the bottom surface of the microwells, allows efficient trapping and stable positioning of single cells for high-throughput biochemical analyses. We demonstrated that various on-chip analyses including immunostaining, viability/apoptosis assay and fluorescent in situ hybridization (FISH) at the single-cell level could be conducted just by applying specific reagents for each assay. Our simple method should greatly help discrimination and analysis of rare cancer cells among a population of blood cells.
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.|JST CREST, Tokyo, Japan.
authors.forename: Marina
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.|JST CREST, Tokyo, Japan.
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.|JST CREST, Tokyo, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.|LIMMS/CNRS-IIS UMI 2820, The University of Tokyo, Tokyo, Japan.|JST CREST, Tokyo, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Kaneda
authors.affiliation: Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.|LIMMS/CNRS-IIS UMI 2820, The University of Tokyo, Tokyo, Japan.|JST CREST, Tokyo, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Fujii
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009369:Neoplasms / Q000473:pathology*; D059010:Single-Cell Analysis