title: The journey of integrins and partners in a complex interactions landscape studied by super-resolution microscopy and single protein tracking.
abstract: Cells adjust their adhesive and cytoskeletal organizations according to changes in the biochemical and physical nature of their surroundings. In return, by adhering and generating forces on the extracellular matrix (ECM) cells organize their microenvironment. Integrin-dependent focal adhesions (FAs) are the converging zones integrating biochemical and biomechanical signals arising from the ECM and the actin cytoskeleton. Thus, integrin-mediated adhesion and mechanotransduction, the conversion of mechanical forces into biochemical signals, are involved in critical cellular functions such as migration, proliferation and differentiation, and their deregulation contributes to pathologies including cancer. A challenging problem is to decipher how stochastic protein movements and interactions lead to formation of dynamic architecture such as integrin-dependent adhesive structures. In this review, we will describe recent advances made possible by super-resolution microscopies and single molecule tracking approaches that provided new understanding on the organization and the dynamics of integrins and intracellular regulators at the nanoscale in living cells.
authors.affiliation: Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Rossier
authors.affiliation: Univ. Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France; CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Giannone
mesh_terms: D002448:Cell Adhesion; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D008853:Microscopy; D036103:Nanotechnology; D021381:Protein Transport; D013314:Stress, Mechanical
chemical_list: D016023:Integrins
keywords: Integrin; Interactions landscape; Mechanotransduction; Single protein tracking; Super-resolution microscopies